Add Colab demo notebook + KaiB demo data

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examples/SF_Cluster_Demo.ipynb +290 -0
examples/data/KaiB_fi_matrix.npy +3 -0
examples/data/KaiB_filtered.a3m +728 -0
examples/data/KaiB_seq_ids.txt +364 -0
examples/data/provenance.md +57 -0

examples/SF_Cluster_Demo.ipynb ADDED Viewed

	@@ -0,0 +1,290 @@

+{
+ "cells": [
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "# SF-Cluster \u2014 frustration-guided MSA subset builder\n",
+    "\n",
+    "**What this notebook does.** Installs the open-source `sf_cluster` package, downloads a small KaiB demo bundle (a 364-sequence MSA + a per-residue Frustration Index matrix from FrustrAI-Seq), and builds two flavours of stratified MSA subsets (`mosaic` and `gradient`) using the contrast-HV/LV score. Everything runs on CPU in roughly two minutes.\n",
+    "\n",
+    "**Who it is for.** Biologists who want reproducible, frustration-stratified MSA slices to feed into an AF-Cluster-style multi-conformer prediction loop.\n",
+    "\n",
+    "**What you do next.** Take the 12 mosaic or 12 gradient A3M subsets emitted at the end of this notebook, run each through ColabFold AF2, and aggregate per the SF-Cluster \u00a79.1 hit criterion.\n",
+    "\n",
+    "---\n",
+    "\n",
+    "> ## LIMITATIONS \u2014 please read\n",
+    "> A controlled comparison on the Main-21 cases shows that **uniform random subsampling performs equivalently on most cases**. The frustration signal is **not** the active ingredient here \u2014 depth reduction is. See the OSS README for the full ablation.\n",
+    ">\n",
+    "> Use this tool when you want **stratified, reproducible MSA subsets** with a clear provenance story \u2014 not as a guaranteed conformational diversity engine. It is a research baseline, not a turnkey accuracy improvement."
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "## 1. Install the package\n",
+    "\n",
+    "Pulls the OSS release from Hugging Face. Pure-Python; only depends on `numpy` and `scipy`."
+   ]
+  },
+  {
+   "cell_type": "code",
+   "metadata": {},
+   "execution_count": null,
+   "outputs": [],
+   "source": [
+    "!pip install -q git+https://huggingface.co/ChatterjeeLab/SF-Cluster"
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "## 2. Download the KaiB demo bundle\n",
+    "\n",
+    "Three files, ~200 KB total: a filtered MSA, a per-residue FI matrix from FrustrAI-Seq, and the parallel sequence-ID list."
+   ]
+  },
+  {
+   "cell_type": "code",
+   "metadata": {},
+   "execution_count": null,
+   "outputs": [],
+   "source": [
+    "from huggingface_hub import hf_hub_download\n",
+    "from pathlib import Path\n",
+    "import os\n",
+    "\n",
+    "REPO = 'ChatterjeeLab/SF-Cluster'\n",
+    "FILES = ['examples/data/KaiB_filtered.a3m',\n",
+    "         'examples/data/KaiB_fi_matrix.npy',\n",
+    "         'examples/data/KaiB_seq_ids.txt']\n",
+    "\n",
+    "local = {}\n",
+    "for fname in FILES:\n",
+    "    p = hf_hub_download(repo_id=REPO, filename=fname, repo_type='model')\n",
+    "    local[fname] = p\n",
+    "    print(f'{fname:50s}  {os.path.getsize(p)/1024:7.1f} KB  ->  {p}')\n",
+    "\n",
+    "A3M = local['examples/data/KaiB_filtered.a3m']\n",
+    "FI  = local['examples/data/KaiB_fi_matrix.npy']\n",
+    "IDS = local['examples/data/KaiB_seq_ids.txt']"
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "## 3. Build the pool and stratified subsets\n",
+    "\n",
+    "The `pool_msa` call ties the MSA records to their per-residue FI vectors. `contrast_hvlv` computes the per-sequence high-variance / low-variance FI contrast (see README for the formula). `method_mosaic` and `method_gradient` then deterministically draw 12 subsets of 32 sequences each."
+   ]
+  },
+  {
+   "cell_type": "code",
+   "metadata": {},
+   "execution_count": null,
+   "outputs": [],
+   "source": [
+    "import numpy as np\n",
+    "from sf_cluster import pool_msa, contrast_hvlv, method_mosaic, method_gradient\n",
+    "\n",
+    "pool = pool_msa(A3M, FI)\n",
+    "print(f'pool: N_seq={pool.n_seq}, L={pool.n_cols}, query={pool.headers[0]!r}')\n",
+    "\n",
+    "score = contrast_hvlv(pool.fi_matrix)\n",
+    "print(f'contrast_hvlv: shape={score.shape}, '\n",
+    "      f'min={score.min():+.3f}, median={np.median(score):+.3f}, max={score.max():+.3f}')\n",
+    "\n",
+    "mosaic_subsets   = method_mosaic(score)\n",
+    "gradient_subsets = method_gradient(score)\n",
+    "\n",
+    "def summarize(name, subsets):\n",
+    "    print(f'\\n[{name}]  {len(subsets)} subsets')\n",
+    "    print(f'{\"subset_id\":>10}  {\"n_seqs\":>7}  {\"mean_contrast\":>14}')\n",
+    "    for i, sub in enumerate(subsets):\n",
+    "        m = float(np.mean(score[sub]))\n",
+    "        print(f'{i:>10d}  {len(sub):>7d}  {m:>+14.4f}')\n",
+    "\n",
+    "summarize('mosaic',   mosaic_subsets)\n",
+    "summarize('gradient', gradient_subsets)"
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "## 4. Visualise\n",
+    "\n",
+    "Three plots: the contrast score distribution with tercile / quartile boundaries marked, the per-subset mean contrast score for both methods, and the pairwise sequence-overlap heatmap between mosaic and gradient subsets."
+   ]
+  },
+  {
+   "cell_type": "code",
+   "metadata": {},
+   "execution_count": null,
+   "outputs": [],
+   "source": [
+    "import matplotlib.pyplot as plt\n",
+    "import numpy as np\n",
+    "\n",
+    "fig, axes = plt.subplots(1, 3, figsize=(15, 4))\n",
+    "\n",
+    "# (a) score histogram with tercile + quartile lines\n",
+    "ax = axes[0]\n",
+    "ax.hist(score, bins=40, color='#4477AA', edgecolor='white', alpha=0.85)\n",
+    "sorted_s = np.sort(score)\n",
+    "N = len(sorted_s)\n",
+    "terciles = [sorted_s[N//3], sorted_s[2*N//3]]\n",
+    "quartiles = [sorted_s[N//4], sorted_s[N//2], sorted_s[3*N//4]]\n",
+    "for t in terciles:\n",
+    "    ax.axvline(t, color='#CC6677', linestyle='--', label='tercile (mosaic)' if t==terciles[0] else None)\n",
+    "for q in quartiles:\n",
+    "    ax.axvline(q, color='#117733', linestyle=':',  label='quartile (gradient)' if q==quartiles[0] else None)\n",
+    "ax.set_xlabel('contrast_hvlv')\n",
+    "ax.set_ylabel('count')\n",
+    "ax.set_title('(a) per-sequence contrast score')\n",
+    "ax.legend(fontsize=8)\n",
+    "\n",
+    "# (b) per-subset mean contrast\n",
+    "ax = axes[1]\n",
+    "x = np.arange(12)\n",
+    "m_means = np.array([score[s].mean() for s in mosaic_subsets])\n",
+    "g_means = np.array([score[s].mean() for s in gradient_subsets])\n",
+    "w = 0.4\n",
+    "ax.bar(x - w/2, m_means, width=w, label='mosaic',   color='#4477AA')\n",
+    "ax.bar(x + w/2, g_means, width=w, label='gradient', color='#CC6677')\n",
+    "ax.axhline(0, color='black', lw=0.5)\n",
+    "ax.set_xlabel('subset id')\n",
+    "ax.set_ylabel('mean contrast_hvlv')\n",
+    "ax.set_title('(b) per-subset mean score')\n",
+    "ax.legend(fontsize=8)\n",
+    "\n",
+    "# (c) pairwise overlap heatmap (mosaic x gradient)\n",
+    "ax = axes[2]\n",
+    "M = np.zeros((12, 12), dtype=int)\n",
+    "for i, si in enumerate(mosaic_subsets):\n",
+    "    set_i = set(si)\n",
+    "    for j, sj in enumerate(gradient_subsets):\n",
+    "        M[i, j] = len(set_i & set(sj))\n",
+    "im = ax.imshow(M, cmap='magma', aspect='auto')\n",
+    "ax.set_xlabel('gradient subset')\n",
+    "ax.set_ylabel('mosaic subset')\n",
+    "ax.set_title('(c) sequence overlap (count)')\n",
+    "plt.colorbar(im, ax=ax, fraction=0.046, pad=0.04)\n",
+    "\n",
+    "plt.tight_layout()\n",
+    "plt.show()"
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "## 5. Write subsets to A3M files\n",
+    "\n",
+    "Each subset is written as a ColabFold-compatible A3M with the query as the first record. Downstream you would feed one A3M per AF2 run."
+   ]
+  },
+  {
+   "cell_type": "code",
+   "metadata": {},
+   "execution_count": null,
+   "outputs": [],
+   "source": [
+    "from pathlib import Path\n",
+    "from sf_cluster import build_subsets\n",
+    "\n",
+    "out_mosaic   = Path('./subsets_mosaic')\n",
+    "out_gradient = Path('./subsets_gradient')\n",
+    "\n",
+    "_, _, _, mosaic_paths   = build_subsets(A3M, FI, method='mosaic',   out_dir=out_mosaic)\n",
+    "_, _, _, gradient_paths = build_subsets(A3M, FI, method='gradient', out_dir=out_gradient)\n",
+    "\n",
+    "print(f'mosaic   -> {len(mosaic_paths):2d} files in {out_mosaic}/')\n",
+    "print(f'gradient -> {len(gradient_paths):2d} files in {out_gradient}/')\n",
+    "\n",
+    "sample = mosaic_paths[0]\n",
+    "print(f'\\nFirst 3 records of {sample.name}:')\n",
+    "with open(sample) as f:\n",
+    "    lines = f.read().splitlines()\n",
+    "shown = 0\n",
+    "i = 0\n",
+    "while i < len(lines) and shown < 3:\n",
+    "    if lines[i].startswith('>'):\n",
+    "        print(' ', lines[i])\n",
+    "        if i+1 < len(lines):\n",
+    "            seq = lines[i+1]\n",
+    "            print(' ', seq[:80] + ('...' if len(seq) > 80 else ''))\n",
+    "        shown += 1\n",
+    "        i += 2\n",
+    "    else:\n",
+    "        i += 1"
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "## 6. Bring your own protein\n",
+    "\n",
+    "The demo bundle is tiny and CPU-friendly. For your own target:\n",
+    "\n",
+    "1. **Build an MSA.** Use the official [ColabFold notebook](https://colab.research.google.com/github/sokrypton/ColabFold/blob/main/AlphaFold2.ipynb) (`mmseqs2_uniref_env` mode) to generate a deep `.a3m`, then filter it (e.g. 25%-gap filter) to obtain `your_msa.a3m`.\n",
+    "2. **Compute the FI matrix.** Run [FrustrAI-Seq](https://huggingface.co/leuschj/FrustrAI-Seq) on `your_msa.a3m` to obtain a per-residue Frustration Index matrix `your_fi.npy` of shape `(N_seq, L)`. **A GPU is required for this step.** See the FrustrAI-Seq model card for inference details.\n",
+    "3. **Re-run the cells above.** Just point `A3M` and `FI` at your files and re-execute from \u00a73 onward. The package will raise a `ValueError` if `N_seq` disagrees between the two."
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "## 7. Next: run AF2 on each subset\n",
+    "\n",
+    "Feed each subset A3M into the official [ColabFold AlphaFold2 notebook](https://colab.research.google.com/github/sokrypton/ColabFold/blob/main/AlphaFold2.ipynb) \u2014 one subset per AF2 run. Aggregate per the SF-Cluster \u00a79.1 hit criterion:\n",
+    "\n",
+    "- C\u03b1 RMSD \u2264 3.0 \u00c5 on the `common_core` residues vs. each reference state,\n",
+    "- mean pLDDT \u2265 70 overall,\n",
+    "- mean pLDDT \u2265 70 inside the `switch_region`.\n",
+    "\n",
+    "**Compute budget disclosure (per `docs/protocol_lock.md`).** The SF-Cluster paper locks AF2 at 3 recycles \u00d7 4 seeds \u00d7 5 models for KaiB / Mpt53, and 0 recycles \u00d7 8 seeds \u00d7 5 models for the GA/GB cases. The GA/GB row was further trimmed to **4 subsets per case** during refinement to stay within the compute envelope. Global seed: `20260422`. Per-case seed = `hash(case_name) mod 2^31`; per-subset seed = `base_seed + subset_index`. All inference uses `templates=OFF`, `relax=OFF`, `dropout=OFF`."
+   ]
+  },
+  {
+   "cell_type": "markdown",
+   "metadata": {},
+   "source": [
+    "## 8. Citation, license, companion repo\n",
+    "\n",
+    "```bibtex\n",
+    "@misc{sf_cluster_2026,\n",
+    "  title  = {SF-Cluster: frustration-guided MSA subset builders for AF2 multi-conformer prediction},\n",
+    "  author = {Cao, Hanqun and {Chatterjee Lab}},\n",
+    "  year   = {2026},\n",
+    "  note   = {Workshop release. Companion code: https://huggingface.co/ChatterjeeLab/SF-Cluster},\n",
+    "  url    = {https://huggingface.co/ChatterjeeLab/SF-Cluster}\n",
+    "}\n",
+    "```\n",
+    "\n",
+    "**License:** MIT. See `LICENSE` in the OSS repo.\n",
+    "\n",
+    "**Companion private dev repo.** Full Phase II benchmark code (DBSCAN baselines, all four arms, evaluation harness, region partition ablation) lives in the SF-Cluster private dev repository. The OSS release here is a slim, dependency-light subset \u2014 only the `mosaic` and `gradient` arms and their scoring function \u2014 intended for reuse, not full reproduction of the benchmark."
+   ]
+  }
+ ],
+ "metadata": {
+  "kernelspec": {
+   "display_name": "Python 3",
+   "language": "python",
+   "name": "python3"
+  },
+  "language_info": {
+   "name": "python",
+   "version": "3.10"
+  }
+ },
+ "nbformat": 4,
+ "nbformat_minor": 5
+}

examples/data/KaiB_fi_matrix.npy ADDED Viewed

	@@ -0,0 +1,3 @@

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examples/data/KaiB_filtered.a3m ADDED Viewed

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+-----LRLYVAGQTSRSLAAIDNLRRICEKNLKGRYTIEVIDLMQAPQLARTDQIVAIPTLVKKLPPPLRRIIGDLSNSERVLIGLDI---
+>5937|scaffold842798_1|-17|01	115	0.453	7.266E-27	2	87	91	22	107	122
+--KWDLRLYVAGPTTKSLAAFRNLEQLCKDHLPGKYHIEVVDLVKTPQLAKGEQILALPALVRQLPIPIRKVVGDLSDTERVLVGLDL---
+>SRR4030095_15264891	128	0.611	2.104E-31	3	87	91	44	128	134
+---YQLRLYVAGQTPKSVLALKNLEQICEEHLQGRYEIEVIDLLQNPQLARGDQILALPTLVRRLPEPIKKIIGDLSNKERVLVGLDL---
+>UniRef100_K9UBR2	49	0.287	3.904E-04	1	73	91	13	85	398
+-KSLQLLLFLDERMTSYVQNQEIRDKLAILNAQDAFELQVVDVGKQPDLAEHYRVIATPALLKLYPTPRQILAG-----------------
+>UniRef100_UPI001E525CD0	52	0.315	4.240E-05	2	73	91	19	90	437
+--PLQLVLFI-DRRPSSFQKLREIRNyLLKLHEQYPFDLQIVDVAEQPYLAEYFKLVATPALLKIHPEPRQILAG-----------------
+>UniRef100_A0A845X7U1	48	0.275	1.011E-03	5	73	91	22	90	388
+-----LLLFVDERSGSQQHTQQILDYLNYLEQEHLFELEVLEVGEHPDLAEHFRLIATPSLVRIYPQPKHVLAG-----------------
+>3300017444.a:Ga0185300_10001144_2	52	0.315	4.110E-05	2	73	91	13	84	457
+--PLQLVLFI-DRRPSSFQKLREIRNyLLMLHEQYPFDLQIVDVAEQPYLAEYFKLVATPALLKIHPSPRQVLAG-----------------
+>UniRef100_A0A349JMI1	49	0.324	3.904E-04	4	76	91	36	109	410
+----QLLLFVDKRLSSREQTRHIRKSLQDLKAEWDFELQIIDVGEQPDLAEHFKLLATPSLLKIHPDPRQTLAGsDLS--------------
+>UniRef100_K9SDH8	56	0.356	1.778E-06	2	73	91	16	87	393
+--PLQLLLFV-DRRPSSREQIRTIRnRLKNLEAETPFALEVIDVGEQPYLAEHFKLVATPALLKICPEPRQTLAG-----------------
+>UniRef100_A0A3M1PH87	54	0.283	8.682E-06	1	73	91	12	84	391
+-PSLQLLLFVDKR-PSSIEKIRQIRnRLKELEADYPFDLQVVDVGEQPHMAEHFRLIATPAIIKIHPEPRQTLAG-----------------
+>UniRef100_A0A1C0V439	54	0.272	1.192E-05	0	73	91	11	84	398
+RAPLQLLLFVDHRSSSWEQAsliQGRLQALKARY---PFAFDIVDVAEQPYVAEHFRLIATPALVKVHPHPRQTLTG-----------------
+>UniRef100_UPI001E4DBDD3	44	0.295	3.304E-02	4	73	91	3	72	369
+----QLLLFVDER-PSSSEYVRLIRDYIEiIKKSCPCQLEVIEIRKQPHLVEHFRLVATPALVKVSPGQKQILAG-----------------
+>UniRef100_A0A969FMT9	63	0.364	8.088E-09	0	73	91	20	92	421
+QATIQLLLFV-DRRPSSWEQMRYIRRYLENTDEQNFSLEVVDVSKQPYLAEHFKLIATPTLLKLYPEPRQMLAG-----------------
+>UniRef100_A0A0M2Q0K7	49	0.281	5.361E-04	4	73	91	18	87	393
+----QLLLFVDNRS-SSQEQMWQVRRTLETLsDPQTFQLEVMNVTEQPHLTEHFKLIATPALIRLYPTPQQILAG-----------------
+>UniRef100_A0A978SUS5	52	0.356	4.240E-05	2	73	91	13	84	383
+--PLQLLLFIDKR-PSSLEQSRQIQRYLqQTKAKHAFALQVIEVGEQPYLAEHFKIVATPALIKIYPEPRQIIAG-----------------
+>UniRef100_A0A930TQ40	52	0.293	5.823E-05	2	73	91	13	86	408
+--PLQLLLFVDER-PLSRERTRQIRKRLQElalNSPHAYTLQVVKVAEQPHLVEHFKLVATPSLIKISPEPRQILAG-----------------
+>UniRef100_A0A2W4XV74	47	0.282	3.593E-03	2	73	91	13	89	395
+--PFKLLLFIDKR-PHSSEQIRQiryhLKELMSRELmnKNQISLEIVDVTHQPDLAEHFKLVATPALVKVSADQHQTLTG-----------------
+>UniRef100_A0A939KSU8	61	0.328	5.425E-08	2	73	91	13	84	391
+--PLHLLLFVDKR-PISGEQIGQIsRQLKELHGDCDYELMVVDVGEQPYLAEHFKLVVTPTLIKIYPEPRQTLTG-----------------
+>UniRef100_A0A8K1ZWU9	47	0.338	3.593E-03	4	73	91	19	88	385
+----QLILFVDKR-AASKRQNQQVQDYLKSiEPSHSWNLQVADVEEQPYLAEYYKLVATPALIKLYPEPRQILTG-----------------
+>UniRef100_UPI001C72C3C0	62	0.342	2.095E-08	2	73	91	19	90	401
+--PLQLLLFVDGR-PQSRQQVQRIRSYLRElETEYSFELQIIDVGQQPYLAEHFKLVATPALIKIHPEPQQTLAG-----------------
+>UniRef100_A0A6P0TIA3	54	0.305	8.682E-06	4	74	91	11	81	119
+----QFLLFV-DRRPASSEKIRQIRQYLERKqGSSSIGLQVIDVGEQPSLVEHFRLVATPALVKIYPEPQQVFTGE----------------
+>UniRef100_A0A5B8NIC7	51	0.252	7.996E-05	2	73	91	1	72	365
+--PLQLLLFIDERSSsqEHLQGIQNyLEALKEDY---PFELQMVNVAEQPHLAENFRLVATPALVKIAPQPRQTIAG-----------------
+>UniRef100_UPI002012D1AC	61	0.405	3.951E-08	2	73	91	13	84	388
+--PLQLLLFVDER-PSSQEQLLRLHN-CIQELKTDYpfELEVVDVGEQPYLAEHFKLVATPALIKIHPPPQQTIAG-----------------
+>UniRef100_UPI001C0312E0	49	0.310	3.904E-04	2	73	91	6	77	384
+--PLKLLLFIDKR-PSSVEQVRQIRQHVEG-LEGEIDpvLEVVDVSEQPYLAEHFKLVVTPTLIKVSAAGRQTLTG-----------------
+>UniRef100_A0A928Z921	53	0.319	3.088E-05	2	73	91	13	84	183
+--PLQLLLFVDGRPSSWEQLRQVLAYLKEKNNEVDWDLKTIKVSEKPYLVEHFKLVATPALIKIHPEPQQTLAG-----------------
+>UniRef100_A0A351L2B1	59	0.356	1.930E-07	2	73	91	13	84	168
+--PLQLLLFVDER-PSSTEQLQQLRQYLERLKADyPLEFEVVDVGAQPYLAEHFKLVATPALIKINPPPRHVLAG-----------------
+>UniRef100_A0A8J7E209	50	0.292	2.071E-04	1	77	91	18	95	383
+-KSLQLLLFVDER-PSSQehikRIQSHLQSLQTEYL---FELEAINVGEHPDLVEHFRLIATPALVKIHPQPRQILAGsDLID-------------
+>UniRef100_UPI00232B6744	50	0.283	2.843E-04	1	73	91	19	91	385
+-KQLQLLLFVDERISTQNHA-QQIQSYLEELkQRDAFDLEILEISENPDLAEHFRLVATPSLVKIYPSPRQVLAG-----------------
+>UniRef100_UPI0018EFA1D6	63	0.342	1.111E-08	2	73	91	13	84	395
+--PLQLLLFVDGR-PKSRQQVQRIRAYLTELKTGyQFELQIIDVGQQPYMAEHFKLVATPALVKIHPEPRQIIAG-----------------
+>UniRef100_U5DJK1	51	0.315	7.996E-05	2	73	91	19	90	404
+--PLQLLLFVDERS-RTQEPMRRIQNYLLRLQQDNaFGLQVIGVEKQPHLAEHYRIVATPSLVRIWPPPRQTLAG-----------------
+>UniRef100_A0A832M402	56	0.301	1.778E-06	2	73	91	13	84	431
+--PLQLLLFVDKR-PSSREQVRQVRSALkERKEECDFEVQFIDVTEQPYLAEYFRLIATPALIKIHPEPRQTLAG-----------------
+>UniRef100_A0A1C0VJA3	49	0.338	3.904E-04	4	73	91	37	106	411
+----QLLMFVDKR-PGSHEQIRLLRKSLETLKTDfAFDLQIIDVGEQPYLAEHFKLMATPCLLKIHPSPRQMLAG-----------------
+>UniRef100_A0A969K2I2	52	0.323	5.823E-05	4	73	91	15	84	397
+----QLVLFVDKR-PSSNQKVRQIRNHLKDLRADYvFDLQIVDVGEQPHLAEYFKLVATPALIKIYPEPRQTLAG-----------------
+>UniRef100_K9X4T7	62	0.328	2.095E-08	2	73	91	13	84	395
+--PLQLLLFIDGR-PKSRQQVQRLRAHLKELEAEySFELQIIDVGQQPHLAEHFKLVATPALIKIHPEPRQVLAG-----------------
+>UniRef100_W7Q8H4	65	0.365	1.206E-09	5	85	91	15	96	100
+-----LILFVTGEAPRSRRAHLNLTAALEASGIGDVQPREIDLLVEPQEAIDFGIFATPALMHIDASGTRRVlYGDLSDEHSLRDFL-----
+>W7Q8H4	68	0.365	1.743E-10	5	85	91	15	96	100
+-----LILFVTGEAPRSRRAHLNLTAALEASGIGDVQPREIDLLVEPQEAIDFGIFATPALMHIDASGTRRVlYGDLSDEHSLRDFL-----
+>A0A1H7MTT5	63	0.341	7.839E-09	5	85	91	16	97	101
+-----LILFVTGEAPRSHRAQRNLAAALDASGISDAPAREIDLLHEPQQAIHFGIFATPALMHIDASGNRRVlYGDLSDEHRLKDFL-----
+>UniRef100_A0A1H7MTT5	62	0.341	2.095E-08	5	88	91	16	100	101
+-----LILFVTGEAPRSHRAQRNLAAALDASGISDAPAREIDLLHEPQQAIHFGIFATPALMHIDASGNRRVlYGDLSDEHRLKDFLSVL--
+>A0A1P8R863	62	0.317	1.478E-08	5	85	91	9	90	94
+-----LILFVTGEAPRSRRARYNLSSALKAAGLDKSPAHQIDLLRDPEQAISFGIFATPALMHIDAAGNRSVlYGDLSDQARLARFL-----
+>UniRef100_A0A1P8R863	62	0.317	2.095E-08	5	85	91	9	90	94
+-----LILFVTGEAPRSRRARYNLSSALKAAGLDKSPAHQIDLLRDPEQAISFGIFATPALMHIDAAGNRSVlYGDLSDQARLARFL-----
+>UniRef100_UPI001CD124AC	61	0.357	3.951E-08	8	90	91	1	84	88
+--------FVTGEAPRSRRAHQNLTTALAASGIGDAAPREIDLLGDPQEAINFGIFATPALMLVDASGRRTVlYGDLSDEHSLKTFLSPLRE
+>UniRef100_A0A2N5Y751	59	0.341	1.405E-07	5	85	91	15	96	100
+-----LILFVTGEAPRSRRAHLNLTAALDETGIDVAPPREIDLLREPQEAISFDIFATPALLhIEGSGHRRVLYGDLSDKQSLKDFL-----
+>UniRef100_A0A2N7UCY5	58	0.337	4.998E-07	5	77	91	15	88	105
+-----LILFITGEAPRSRRAQQHLKTALAASGADLAPAREIDLLSAPQEAIDFGIFATPALMIIDASGKRQVlYGDLSD-------------
+>MGYP001039280987	63	0.333	7.839E-09	5	90	91	14	100	104
+-----LMLFVTGNAPRSVRARRNLAGALDSLDLDDVKPMEIDLLSQPEQTVAYSVFATPALLKTEARGKMSVlYGDLSDEGKLQDFLQNLPE
+>UniRef100_U5T3B0	61	0.341	5.425E-08	5	85	91	3	84	91
+-----LILFVTGNAPRTQRARANLAKMLEQIGRGDLGPHEIDLLKQPQEGLTYSVFATPSLLKTDDgGGGSLLYGDLSDSDRLHRFL-----
+>UniRef100_A0A540VSD6	64	0.304	2.274E-09	5	85	91	3	84	96
+-----LILFVTGNAPRTVRARANLSRVLREMGLDSIKPQEVDLLETPQEGLTYSIFATPSLLRTdSGGDEGLLYGDLSDTDRLRRFL-----
+>UniRef100_A0A2S6G6T8	61	0.337	2.877E-08	4	85	91	13	95	102
+----VLLLFVTGTAPRSQRARVNLARMLEQVGRDDIQPHEIDLLEKPKEGVKHSVFATPALMKVGQGGdVSVLYGDLSETDRLQQFL-----
+>UniRef100_UPI00124EBEF7	65	0.337	1.656E-09	4	85	91	13	95	103
+----VLMLFVTGTAPRSQRARTNIAKMLEQLNCTDIQPYEIDLLEQPEQGIKHSVFATPSLLKVSPtGGVSVLYGDLSEEDRLRRFL-----
+>UniRef100_UPI001439CAB5	64	0.350	4.289E-09	5	80	91	15	91	100
+-----LVLFLAGDAPRSRRAHRNLSAALAATCPDLAPVHEIDLLREPQQAIDFGIFATPALLHIDAEGNRRlLYGDLSDEGR----------
+>MGYP000294044467	63	0.317	1.077E-08	5	85	91	3	84	96
+-----LILFVTGDAPRSRRARANLSNMLERLGRSDLTPREVDLLDQPQAGLSYSVFATPSLLKaDQQSDGALLYGDLSDEQRLERFL-----
+>UniRef100_A0A4Q8CZA3	61	0.317	3.951E-08	5	85	91	14	95	104
+-----LILFVTGNAPRTQRARANLARMLEEIGRDDLTPYEIDLLQQPQEGLTYSVFATPSLLKTDDEhGGSLLYGDLSDDDRLYRFL-----
+>3104|Ga0306908_1123748_1|-11|01	68	0.329	9.241E-11	5	85	91	17	98	106
+-----LMLFVTGTAPRSQRARTNLVHMLEQLNRTDVQPYEIDLLEQPEQGIKHSVFATPSLLKVSPTGEVSVlYGDLSEEDRLRRFL-----
+>UniRef100_UPI00133045FA	64	0.329	4.289E-09	5	85	91	14	95	107
+-----LILFVTGDAPRSRRARANLASMLERLGRTDLSPQEIDLLDQPQAGLSYAVFATPSLLKREPErDGALLYGDLSDSDRLERFL-----
+>UniRef100_UPI00201FFA50	61	0.317	2.877E-08	5	85	91	13	94	99
+-----LILFITGTAPRSQRARSNLGKMLDRLNLNDVKPFEVDLLEQPDQGIEHGIFATPSLLKFDSSGEVSIlYGDLSVEERLQKFL-----
+>UniRef100_A0A845V233	60	0.312	1.023E-07	7	85	91	1	80	86
+-------LFVTGSAPRSRRARKNLAAALKSLGLDSVKAMEIDLIDRPEKTVTYSVFATPALLRMDEAGEMRVlYGDLSDESKLLEFL-----
+>UniRef100_UPI00082AD250	59	0.317	1.930E-07	5	85	91	14	95	105
+-----LVLFVTGNSPRSLRARANLAKAVEETASDHITVRHVDLLEDTGGITEYGIFATPALVHVRDGGePAVLYGDLSNEAELQRFL-----
+>MGYP001134272031	63	0.321	5.708E-09	5	90	91	14	100	104
+-----LMLFLTGDAPRSVRARKNLSGALDKLELDEVTPMEIDLLDQPEQTVAYSVFATPALLRTDALGEMSVlYGDLSDEDKLHDFLQNLPE
+>UniRef100_UPI00037CF37C	66	0.337	8.781E-10	4	85	91	13	95	103
+----VLTLFVTGSAPRSQRARANLARMLEQIGRSDMQPQEIDLLEQPEQGITQSVFATPSLLKTDTHGEVSVlYGDLSEEEKLRRFL-----
+>UniRef100_UPI0003674D18	66	0.325	6.394E-10	4	85	91	13	95	103
+----VLTLFVTGSAPRSQRARANLARMLEQIGRSDLQPQEVDLLEHPQQGITQSVFATPSLLKTDANGEVSVlYGDLSEEEQLRRFL-----
+>UniRef100_UPI00047687D0	61	0.313	2.877E-08	4	85	91	13	95	102
+----VLTLFLTGTAPRSQRARANLAHMLEQIGRSDLRPYEIDLLEQPEESITHSVFATPSLLKTSDtGGVLMLYGDLSDEDTLHRFL-----
+>UniRef100_A0A3S0W7L6	59	0.324	1.405E-07	5	80	91	3	79	92
+-----LTLFVTGTAPRSQRARVNLAQMLNRIGRSDIQPYEIDLLEQPGQGITHSVFATPSLVKANeTGEVSVLYGDLSDEER----------
+>UniRef100_UPI001903F48C	63	0.329	1.111E-08	5	85	91	14	95	99
+-----LVLLVTGDAPRSRRARQNLARALEQLGLGDIATREVDLAADPAQTLSYGIFATPALLRPGPnGQPDVLYGDLSERDMLERFL-----
+>SRR4051794_37995438	82	0.354	1.920E-15	7	85	91	61	139	143
+-------LFIAGDGPNSTAAVANLRAFLAQRSASHVEVEIIDVFMEPQRGVSASVFVTPMLVRVEPTPERRILGNLSDRTVLASVL-----
+>UniRef100_A0A318VX16	61	0.301	2.877E-08	4	85	91	14	96	104
+----VLTLFVTGSAPRSQRARANLARMLEQLGRADLRPREVDLLEQPLQGITQSVFATPSLLKTDTNGEVSVlYGDLAEEEQLRRFL-----
+>UniRef100_A0A372BWN2	67	0.337	2.469E-10	5	86	91	14	96	101
+-----LTLFVTGDAPRSRRARRHLNAALKKLGQDSIKPLEIDLLEHPEQSINHSVFATPALLRARNDGEISVIyGDLSDESKLLDFLG----
+>3300017992.a:Ga0180435_10008823_6	74	0.379	7.937E-13	0	86	91	10	96	103
+RSRYVVRLFVAGDAHNSRIARENLNQLRDLLNDTELSIQVIDVEENPQLAIEHSIYVTPALQIVEPKPPTLVYGNLRDKETLLALFE----
+>UniRef100_UPI000401CEFF	70	0.325	2.681E-11	7	86	91	18	97	110
+-------LYIAGDAPNSRIALQNIKMIQENISQWNLKVAIVDVVATPEVALEKGIYLTPALEIEAHGMESLVYGNLSDKEKILALFG----
+>16161|scaffold59688_2|+220|00	84	0.353	3.934E-16	0	81	91	11	92	102
+KRRYVLRLFVAGDAPNSRIARENLRRLQESVAECDFEVEIVDVMENPQSALDHGVFVTPALQIIEPGPEKLIFGNLTNKEAL---------
+>SRR5690606_35087643	76	0.305	3.066E-13	3	87	91	39	123	125
+---YVMRLFISDNAVNSRIARENLSNFLAEFPQHSFQIEIVDLYLQPEMALQNGIFITPTLQILAPQPGGIIYGNLSDRNALERILQI---
+>UniRef100_A0A0R3M5F3	93	0.404	2.017E-19	4	87	91	10	93	100
+----VLRLYIAGNSAGSRRAEQNLHDLRALLDNQAWRIEIIDVMRRPELAEQAGIIATPTLSCEHSGRPRRIVGDLSDKKRVLEFLGI---
+>UniRef100_A0A969HBU9	81	0.352	2.720E-15	1	85	91	12	96	101
+-KHYVIRLFVAGNAPNSRLARENLDRFQAGFPEHEFKVEIIDLDIQPELALENGVFITPTLVVLEPAPGGMIYGNLSDQKVLAQVL-----
+>MGYP000847580960	74	0.284	1.090E-12	0	87	91	10	97	98
+KKRYALRLFVAGNATNSRIARENLEQLLARHPEHEFEVEIVDLNVQPEFALDQGVFISPALQILEPSSGGIVYGNLSQKEVLEKVLNL---
+>A0A0R3M5F3	91	0.395	1.797E-18	4	89	91	10	95	100
+----VLRLYIAGNSAGSRRAEQNLHDLRALLDNQAWRIEIIDVMRRPELAEQAGIIATPTLSCEHSGRPRRIVGDLSDKKRVLEFLGIET-
+>SRR5262249_39096779	91	0.469	9.533E-19	5	87	91	44	126	134
+-----LRLYIAGNSAISRRAEQNLLHLQSLVKPGAWEVHVIDVLPKPELAEQAGILATPTLSYEHPVRPRRIIGDLSDTTRVLDFLGI---
+>SRR5690348_3124064	88	0.481	1.652E-17	5	87	91	48	130	138
+-----LHLYVAGNTASSRRAQQNLLRLREIMKEPQCEVRIIDVLVEPQLAEEAGILATPTLSYEHPQRPRRIVGDLGESKRILEFLGL---
+>MGYP000666260026	97	0.404	8.218E-21	4	87	91	8	91	97
+----VLRLYVAGEGPNSVRARANIVDLCDRHLQGAYSLEIVDVFDEPGRALEEGVLMTPMLVVASASPPRRVVGTLDETSVVLTALGL---
+>UniRef100_A0A838IZY5	88	0.392	1.705E-17	5	86	91	11	94	101
+-----LRLYVAGEGPNSRQARENLRVICEAHLAGRHVIEVLDVFEEPERALDDGVYLTPQLLVLVlpPATPRTVVGNLSEREVVLRALG----
+>MGYP001366537082	74	0.301	7.937E-13	4	86	91	16	98	121
+----VIRFFVAGEAPNSIIARDNLRRLRESLPEIHFEIEIVDVNVNPEIALQKGVFVTPALEVLEPPPGGIFYGNLSNSDPIRRLIE----
+>A0A1Q2HNV8	77	0.394	8.623E-14	2	77	91	6	81	93
+--KMVLRLYVAGKGLNSAMAIENLKQICRTCNSYDYDLKIVDVLKEPQTALDKGIFVTPALEIIEPAPGGMVYGTLAD-------------
+>UniRef100_UPI002011AF87	91	0.471	1.854E-18	5	90	91	36	122	127
+-----LRLYIAGPSATSRRAEQNLRRLRDvAKARDGLAVEIIDVLKNPELAEQAAIIATPTLALEHPVRPRRIIGDLSDVERVLDFLGIESE
+>23258|scaffold4609030_1|+1|11	75	0.337	5.780E-13	6	85	91	20	99	104
+------RLFVCGDALNSRRARENLQRLREMFPHVEFKVEVIDVGETPQAALDQGIFVNPALQVLEPGPGMLIYGDLSDLQALAAML-----
+>SRR5579871_6120579	90	0.435	3.387E-18	5	89	91	48	132	138
+-----LRLYIAGNSASSRRAEHNLEHLRKFMNAEGWKIEVIDVLARPELAEEASILATPTLSYEYSGRPRRIVGDLSDTKRVLKFLGIEP-
+>MGYP000105995723	87	0.428	4.277E-17	4	87	91	2	85	93
+----VLRLYIAGNSPSSRLAQQNLKHLRMLMKGGNEQVEVVDVLANPELAEKASILATPTLCYEHSGRQRRIVGDLGDPKRILAFLGI---
+>SRR3954468_6301146	102	0.431	2.518E-22	0	87	91	46	133	148
+KAPMVLRLYVAGDAPNSTRARANLRRLLSAVDPSRYNLEVIDFLTEPLRALDDGVLVTPTLMRVDPPPPQVVVGTLSALDRVADALDI---
+>MGYP001433622665	94	0.494	1.037E-19	1	87	91	3	89	94
+-KPYQLMLFVAQGQPNSVRAQKNLRQICEEVIPGKYHLKVIDVVKEPELAVENGIYLTPMLVVSDPPPPASITGDMAERKTVLAALKI---
+>UniRef100_A0A3D6C093	96	0.494	2.194E-20	1	87	91	3	89	94
+-KPYQLMLFVAQGQPNSVRAQKNLRQICEEVIPGKYHLKVIDVVKEPELAVENGIYLTPMLVVSDPPPPASITGDMAERKTVLAALKI---
+>A0A1W6LJH8	78	0.418	6.280E-14	4	77	91	8	81	93
+----VLRLYVAGKNVNSTLAIENLEKLCRRCNSFEYDLKIVDVLKNPETALEKGIFVTPALEILEPAPGGMVYGTLSD-------------
+>SRR5687767_11767969	91	0.404	1.797E-18	4	87	91	38	121	122
+----VLRLYIAGNSASSRRAEQNLHALRASLAQNAWEVEIIDVLSKPELAEQAGVIATPTLSYEHSGRSRRIIGDLSDKKRILEFLGI---
+>SRR4051794_40104329	92	0.459	5.058E-19	5	90	91	41	127	130
+-----LRLYIAGPSATSRRAEQNLLRLRDvAKAPNGLEVEVIDVLENPELAEQAAIIATPTLAFEHPVRPRRIIGDLSDVERVLDFLGIESE
+>SRR3954454_22706284	106	0.441	1.060E-23	2	87	91	11	96	109
+--PLVLRLYVAGDAPNSARARANLTRLLSDLDSSRYTLEIIDCLDEPARALGDGVFVTPTLVRLGPPPQRTIVGTLSATDRVADALDL---
+>MGYP000010225417	75	0.407	4.209E-13	5	85	91	104	183	188
+-----LVLYVAGDGPYSRRARANLQALMRE-AGIAAEVTVVDVLKSPDRALEHGIFATPALIVVHGKHETLIMGDLSERDTALEAL-----
+>2271|Ga0209795_10171170_2|-245|01	90	0.406	2.467E-18	4	89	91	30	115	121
+----VLRLYIAGNSASSRRAEQNLHRMQAFIKSEAWDVEIIDVLSKPELAEKAGIIATPTLSFEHSARPRRIVGDLSDTKRVLEFLGIET-
+>MGYP000738482073	88	0.329	1.652E-17	1	90	91	15	105	111
+-PIYRLRLFIAGDEPNSVRAREALARLRNERLGPQCEVEVVDVFQDYQAAITHGVSVVPTLKIEGPRGGRTIVGSLRDEAVVLAALGLsPTE
+>SRR6202012_1017248	94	0.447	1.037E-19	3	87	91	12	96	104
+---FSLRLYIAGDSITSRRARQQLARIREILKQHKFDVETIDVLAQPQLAEQERILATPTLASEHGGPPKRIVGDLSDTKRVLEFLGI---
+>26133|Ga0268298_10010625_3|-7238|00	74	0.317	1.090E-12	3	87	91	15	99	100
+---YALRLFVAGNAANSQIARENLERLRARYPDYEFEVEVIDLNIDPEVALTHGIFISPALQVIDPPTGGVIYGNLSDERVLERVLKL---
+>SRR5262249_6174883	86	0.452	5.872E-17	4	87	91	14	97	102
+----VLRLYIARNSPSSRRAEQNLDYLRRLMKADGGRVEVIDVLANPELAERESILATPTLCYEHSGHRRRIIGDLGDPERILAFLGI---
+>UniRef100_UPI001904043C	77	0.383	8.896E-14	2	87	91	2	86	90
+--PYQLRLFVSGPNPLCRKAERAIRELLIERGV-AYELDVIDVLADPDAAEEYALVATPTLECTAPPPVRRVVGYYEHYAEVFDALGI---
+>MGYP001146183833	101	0.583	3.457E-22	4	87	91	2	85	90
+----ELRLYVIGKTPSAIKATEHLRALLEDQYKDEYALEVVDVLENPILASDDKILATPTVVRRLPHPIRKVIGDLSEREKVLLGLDL---
+>UniRef100_A0A2U2N9L6	76	0.329	2.303E-13	4	85	91	7	88	93
+----QLTLFVAGDSPRSRHAREVLRRALAERGLDPGALELVDVLAEPERTLEHGVFATPALVLRADGATRSLYGDLSDEQGLQQFL-----
+>UniRef100_A0A127EN01	85	0.433	1.568E-16	5	87	91	11	92	99
+-----LRLYIAGRSAISQRAESHLRQ-LHRSIKLECNIEIIDVLKSPELAEQAGVLATPTLSYEHPSRSRRIIGDLSDTKRIVEFLGI---
+>A0A127EN01	87	0.421	4.277E-17	5	87	91	11	92	99
+-----LRLYIAGRSAISQRAESHLRQL-HRSIKLECNIEIIDVLKSPELAEQAGVLATPTLSYEHPSRSRRIIGDLSDTKRIVEFLGI---
+>U2E7T8	67	0.387	3.286E-10	7	85	91	13	92	100
+-------LFVAGDSPSSRRARRALESLIGSQSNEQkAQFEVVDVLREPERALESNLLATPTLLIERGGHVSRYVGDLHEREDVREEL-----
+>UniRef100_UPI00190730C9	67	0.308	3.391E-10	5	85	91	15	95	97
+-----LTVFIAGDAPSSRQAMAHLTGVLDSIGIPPERLQVVDVLTDPGAALDAGALVTPSLQIKRGERARWFLGDLTDQRDLLAFL-----
+>UniRef100_UPI000D3E5BC6	66	0.347	6.394E-10	2	73	91	3	74	103
+--RFRVNVYVVGGSNHASRAVALLRDVADTHFGGDAEITVIDVTSEPALADAAGVITTPTYDLLAPLPRRRIIG-----------------
+>UniRef100_A0A1Y6FIV8	61	0.308	5.425E-08	5	85	91	11	91	95
+-----LTLLVAGESQATRSARATLDSLIGDGLAEASHVRVIDILQQPDYALRYKAFFTPSLIVETPTTTTTIVGDLHELDEVRSLL-----
+>SRR5919109_3969706	72	0.309	5.322E-12	2	85	91	7	90	100
+--PVSLVLYVSAESPASQRARRHLESLLAQFDASQLAVEVCDVSADPVRGETDHVVFTPTLVARSGGLATWVLGDLADRSMLVDLL-----
+>MGYP001077603090	119	0.505	1.625E-28	1	87	91	17	103	106
+-EKWNFTLYVAGDNLSARRAKKNLQGICDEYLEGRYAIEIVDLVEHPEIAEEDQILAAPTLVRKLPLPLRRIIGDLSSREKVLIGLEI---
+>SRR3954463_15126113	100	0.392	8.943E-22	3	86	91	17	100	101
+---FKFRLYVASSTPNAAKATANLQQLCREHLPGRHAIEVVDVFKQPKRALADQIYLTPTLLRLAPMPVRKIVGNLSEASALLAALG----
+>ERR1039457_666370	113	0.443	3.540E-26	2	89	91	12	99	108
+--RYRFKLYVTDHTLRSRQALAQLRKLCDEQFPQQYELEVVDVLEHPDEAAAQHIFATPTVVRERPLPIRRVIGDLSDMGKVLAGLALPP-
+>UniRef100_UPI0005BD3569	63	0.370	8.088E-09	7	84	91	7	86	98
+-------LFVAGGAPRSAAALRNLTAAIAatGRPEGTFRIELIDVLRDPARALEAGLLATPSLALTaANGRRRWFIGDF-DRPELLAG------
+>10876|scaffold_592705_c1_2|-157|01	111	0.425	9.155E-26	1	87	91	56	142	164
+-PVWKLHLYVADTTPRSVLATENLHSFCDQYLPGQYRVTIIDIVKQPALAREHEILATPTLIRVFPGPERTVVGSLSDTARVARALEL---
+>UniRef100_A0A7V9DA05	104	0.360	5.330E-23	3	88	91	3	88	99
+---YSFRLYVTGETTLSREAEANLRALCKNRLVDDYEIEIVDILERSALAEEEQIVATPTIMRLAPLPRLRVIGDLSDHERAARAFGLP--
+>MGYP000147404972	93	0.414	2.683E-19	7	88	91	17	98	99
+-------LFVAGNAPNSVSAQANLRQVCEQRLKNGWELKIIDVLEDYGTALDHGILVTPALVILEPLPAVTVFGDLSDTDRLLKALRLI--
+>SRR5207249_9234194	77	0.373	8.623E-14	3	85	91	34	115	125
+---FELVLYVSPGSPACARAQRNIHELLGRLDRAQVDLDVRDVSEDAERAEADRILFTPTLVVRRPL-LTWIVGDLTNGEEVLRVL-----
+>UniRef100_A0A8T3N6J2	109	0.500	4.603E-25	2	87	91	1	86	97
+--KYRLRLFVTGHTPASLSAQKNLRKLCEGELRGWCEFEVVDVLKQPELAEEARIIATPTLVKLTPEPQRKVIGDLSNHDQLLHVLDM---
+>SRR3954451_13963006	68	0.325	1.269E-10	5	87	91	69	149	159
+-----FTLYVDGPE-QGRHVSRRLLELCQPWGIAP-DLSVVDVGDGPDQAEQANIIGTPTVVREAPAPRRRIIGALDDDRRVVEALGL---
+>UniRef100_A0A1T4Y342	59	0.308	1.930E-07	5	85	91	11	91	95
+-----LTLLVAGESSAARSARATLDTLIGDGLAEASHVRVIDVLQQPDYALRYKVYFTPSLIVETSTTTTTIVGDLHEIDEVRSLL-----
+>UniRef100_A0A2V7TZK6	77	0.373	1.222E-13	3	85	91	21	102	112
+---FELVLYVSPGSPACARAQRNIHELLGRLDRSQVDLDVRDVSEDAERAEADRILFTPTLVVRRPL-LTWIVGDLTNGEEVLRVL-----
+>UniRef100_A0A831PRG7	79	0.301	1.823E-14	4	86	91	30	112	122
+----HLRLYILGTSARASLARQRVEEFCGQFPPGRLRLEVIDLLVDGEVAERDRIIATPSLRRVMPLPVVSLVGDMGDEQQLVALVN----
+>SRR6185295_5137210	81	0.372	2.636E-15	0	85	91	100	184	194
+RRPVELVLYVSPASPASARAQHNIHELIARLGGSKVDLDVCDVSEDAERAEADRILFTPTLVVRRPL-LTWIVGDLTNGEEVLRIL-----
+>SRR5687767_10342612	86	0.380	8.062E-17	2	85	91	60	142	156
+--PVELALYVTLPWPASLKAKRNLDRVLSGFSRSQVSLTVCDLAQEPERAEQDGIVFSPTLVKRMPEPRAWVMGDLSDR-KVLSNL-----
+>SRR5881409_182087	64	0.290	4.157E-09	2	87	91	5	88	111
+--PMVFTAYVDG-TEMGGQVRTRLLELCASRDVTA-EIRVVDVLSEPAAAETGNVVGVPTVVREQPHPRRRVIGVLDDTRRVAEALGL---
+>UniRef100_A0A4Q3W5A8	102	0.459	1.893E-22	1	87	91	12	98	102
+-KEFVLRLFVTGASPNSLKALNNIREICENHAKGNYSLEVIDVYQNAELVQQEQIIALPLLVRKNPLPERKLIGDLSEKEKVIKYLGL---
+>UniRef100_A0A934QHJ2	66	0.320	8.781E-10	5	85	91	16	96	98
+-----LTVFIAGDAPSSRQAMTNLTGVLDSLDIPPERLEVVDVLTNPAAALKAGALVTPSLQVKRGQQVYWFLGDLTEQRDLLAFL-----
+>4460|scaffold_415991_c1_2|-159|00	113	0.448	2.579E-26	0	86	91	24	110	118
+KARYTLRLYVAGFRQSSRSAIANIRRICDKHFEGSANLEVIDIYQQPELAAAQQIIASPTLIKEAPAPFRRVIGDLSDTTKVLAALG----
+>MGYP000603749840	111	0.443	1.725E-25	2	89	91	69	156	158
+--RFLLQLYVAGNSHRCVNARKNLREICEEHLPDSYTLEIIDIVENPEAAEEADIVAVPTLVKRSPSPVRKVVGDMSRTQNVLSGLNIEP-
+>UPI0003E01BF4	75	0.317	4.209E-13	2	85	91	167	251	261
+--RIELALYISASSPSSLKALRNLTRLLADHDAAQVRFTTYDLSkEHIAAAQEDRIAFTPTLVKRWPEPKVWILGDLDDIRVVSDLL-----
+>10796|Ga0318514_12978242_1|+2|11	63	0.291	5.708E-09	0	77	91	12	89	92
+QPRVELSLYTSSGSPSSLKAVRNLMSLLSNYDPLQVRLSVRDLsREAHEQAAEDRIAFTPTLVKRN-EPKVWVLGSLDD-------------
+>UniRef100_UPI0021E125E4	71	0.313	1.035E-11	1	85	91	139	224	234
+-KRIAFTLYISEASTASLRALRNLQKLLDGYDASQIDLRVVDLSkERPASFDEDRITFTPTLVKRSPEPRVYLLGTLEHIQSVADLL-----
+>SRR3712207_3115678	67	0.311	2.393E-10	2	77	91	61	137	142
+--RLELFFYVSSASACSLKALRNLDRFLADYQGAQVRLRVFDLSQDyPAEAEEDRIAFTPSLVRRYPTPKTWLLGSLDD-------------
+>SRR6185295_1711097	83	0.388	1.018E-15	2	85	91	94	178	188
+--RVQLTLYISTSSPSSLKALRNLQKLLNDYDPGTVSLTVCDLSRDtTGSAEEDRIVFTPTLVKRVPEPKVWILGDLENAEIVSDLL-----
+>UniRef100_UPI00214A28C1	63	0.337	8.088E-09	5	86	91	139	221	230
+-----LMLYISEASPASLRALRKLEKLLAGYERSQVRLTVVDLAkERPPSFDEDRIAFTPTLVKRYPTPRAYYLGALDQLQAVTDLLN----
+>UniRef100_UPI00193BA132	68	0.337	1.798E-10	5	86	91	139	221	230
+-----LTLYISEASPASLRALRNLEKLLANYERSQVRLAVVDLSkERPPSFDEDRIAFTPTLVKRFPAPRAYYLGTLDQFQAVTDLLN----
+>SRR5688500_8380976	82	0.345	1.398E-15	2	85	91	168	251	262
+--PIELVLYYTPPWPSSMKARRNLEKILGKYEADAVRLTVRDLGEHPDLAEADGVVFSPTLLKKSPGAPVWMLGDLSDATAVTDLL-----
+>ETNmetMinimDraft_32_1059908.scaffolds.fasta_scaffold895325_1	83	0.365	5.401E-16	4	85	91	130	211	221
+----ELVLYYTPPWTSSLKALRNLEKILEGFDKDAVHLNVRDLAEHPEQAEADGVVFSPTLVKKAPGPPVWMLGDLSDSRAVTDLL-----
+>SRR5512132_2975018	81	0.341	4.970E-15	4	85	91	33	114	125
+----ELVLYYTPPWPSSMKARRNLEKILEGYEAEAVHLTLRDLGDHPDLAESDGVVFSPTLIKRSPGAPVWMLGDLSDGSAVTDLL-----
+>13960|scaffold210726_2|+957|01	84	0.341	2.865E-16	1	85	91	155	239	249
+-PKAEFVLYISSASPSSLKALRNMQRLLGEYQASQVRFTVCDLLKEPGCFEEDHVAFTPTLVKRLPGPKTWIVGDLQDSSMVTDLL-----
+>12613|JGI10216J12902_106548506_1|+3|10	64	0.365	4.157E-09	5	85	91	84	164	178
+-----LTLYVS-DSMLSLRAAKNLRMVLARYRDEQVALTVINLSHDVDhHAEEDRIVVTPTLLRTFPAPRVWLVGNLDKRDLVERLL-----
+>SRR5688572_30282090	83	0.341	7.416E-16	4	85	91	58	139	150
+----ELILYYTPPWPSSIKARRNLEKILEGYDADAVHLTVRDIAEQPDLAEADGVVFSPTLIKKSPGAPVWMLGDLSDASGITELL-----
+>SRR5919106_6413091	63	0.320	7.839E-09	5	81	91	24	100	104
+-----FVMYVNG-SNRSRRALRKVRALFDEYDAAQLTWSTIDVSSDtASRVEQDRIVVTPTLLKTYPSPAVWITGELENTDVV---------
+>SRR5688572_2308330	79	0.317	2.426E-14	4	85	91	137	218	229
+----ELVFYYTPPWPSSMKARRNLEKILSGYAADAVHLTVRDLGEHPDLAEADGVVFSPTLIKKSPGAPVWMLGDLSDASGITELL-----
+>3300018984.a:Ga0193605_1004274_5	73	0.318	2.055E-12	0	86	91	168	255	265
+QKRIGFVLYISEASSSSLRALRNLQRLLDEYETSQIDLKVVDLSkERPASFDEDRITFTPTLVKRNPEPRVHLLGTLEHIQSVAELLG----
+>GraSoiStandDraft_41_1057321.scaffolds.fasta_scaffold894400_3	78	0.346	6.280E-14	5	79	91	90	164	168
+-----LVLYVSAASPASIQARRNLERMLSRFEPGQVRWTVRDLEREPLAGEEDRIAFTPTLVKRFPEPRMWVLGNLREAD-----------
+>UniRef100_A0A7X0U868	87	0.345	2.341E-17	4	87	91	21	104	113
+----HFRLYVSTTSPISLRAIANARRILQEAYPGAHRLTVLNIAEHVALARTDQIIVSPTLLRLAPLPQRRFIGDLSDLNRLRRALGM---
+>SRR5690242_21852208	83	0.395	1.018E-15	2	86	91	14	99	108
+--RIELVLYTAGRSPASMRALRQMKNLLAQYESAQVDFKVFDLAEgRPASAEEDHILLTPTLVQRSPPPRTWVVGDLEDTTLIADLLD----
+>SRR4051812_14954157	76	0.301	1.626E-13	2	83	91	38	120	132
+--RLELVLYISAQSPASLRAIRNFQAILNEFDADEVDYSVCDLATDiSGAADEDRIAFTPSLVKRHPLPNEWFLGDLTNTDPIRA-------
+>SRR5687768_5535903	80	0.329	6.824E-15	4	85	91	139	220	230
+----ELLLYYTPPWPSSMKARRNLEKILKAYEADAVHLTLRDLGEHPDLAEQDGVVFSPTLIKRSPGAPVWMLGDLSDSTAVTDLL-----
+>GraSoiStandDraft_2_1057267.scaffolds.fasta_scaffold1451175_1	77	0.317	8.623E-14	4	85	91	155	236	246
+----EFVLYVSASSAASSQARRNFEQLLEGYDATQVRYTVCDLGRDPLAGDEDRVAFTPTLVKRYPPPRMWLIGNLRELEIVADIL-----
+>SRR6185437_3447168	72	0.307	5.322E-12	2	79	91	62	139	141
+--PIELMLYVSRHSAHSAQAIRNITSVLSRFKATQVKLTVCDLSADPGAGAKDNITYTPTLVRSGPGPRTYILGHISNPE-----------
+>SRR4051794_7772101	82	0.376	1.920E-15	2	85	91	91	175	188
+--RIELVLYVSGSSPSSLKAMRNLDGVLRQFDLACIQLEICDLSRgYPATAEEDRIAFTPTLVKRGPAPRAWVIGNLENRDLVADLL-----
+>SRR5829696_6517560	79	0.294	1.767E-14	2	86	91	36	120	128
+--KVELVLYISSASPSSIVARRNLEKVLDRFDSAQIHVTVCDLVADPLAGERDRIAFTPTLVKTYPAPKMWVLGNLRDPAIVEDLLG----
+>MGYP000536513446	72	0.362	3.875E-12	5	81	91	139	218	236
+-----LKLYFSGVSTESRRAVRNLRRVLKEFDRHRIRLDVHDISDRAAPVaplEQDRIVVTPTLVRKHPLPKLWILGDLSKLEVV---------
+>SRR5687768_1210620	76	0.358	2.232E-13	5	81	91	136	216	217
+-----LALYVSGTSPSSRKALRNLTQVLRNVEPHRVAVTVHDISEsdHPwvEAAEDDRVVVIPTLVRHAPLPRVWIAGDLSEIDTV---------
+>SRR6059058_1924189	81	0.378	2.636E-15	2	75	91	28	101	104
+--RIELVLYVSASSPPSVRAVANLRRILQRYKSDRIRCSICDLTANPEEGDVDQIAFTPTLCKRQPEPPMWILGDL---------------
+>SRR5688500_14284300	87	0.383	4.277E-17	2	86	91	26	111	119
+--RIELVLYVAGPSPASTRALRQMKNLLAQYDAAQVDFRVIDLAQgRPASAEEDRVLLTPALVRRSPLPRTWVVGDLEDTTLVADLLD----
+>SRR5688572_7102903	68	0.373	1.269E-10	5	75	91	47	121	122
+-----LVLYVSGDSQASRRALRNVRRALEGVDPAAIELDVRDVSSSdgaaIAAADADRIVVTPTLVRAAPSPKVWIAGDL---------------
+>SRR4029453_1913048	75	0.261	5.780E-13	2	85	91	54	137	147
+--RVEFVLYVSPNSAASLQARRNFDKLLARFDATQVKYSICDLIRDPLAGDSDRVAFTPTLVKRYPAPRMWLIGNLRETEVLADIL-----
+>SRR6185503_893473	70	0.360	2.599E-11	2	87	91	34	118	126
+--PIELVLYVAPNSPACVRARANLEAALDAYDRSRIRLTVCDVSRDFEDAERDRIVFTPTLL-LRGAEAGCVVGDLSLGDAVDALLSL---
+>SRR5262245_8274589	79	0.285	1.767E-14	2	85	91	152	235	245
+--RVELVLYVSPSSPACAQARHNLERVLDHFDPSQIKYSVFDLVRDPLAGEDDRVAFTPTLVKRYPAPRTWVLGNLRDTQIVGDLL-----
+>SoimicMinimDraft_3_1059731.scaffolds.fasta_scaffold2181823_1	76	0.292	3.066E-13	4	85	91	147	228	238
+----ELVLYVSSASPACIQARRNLEQLLEKFDVSQVRFSICDLGREPTAGDADRIAFTPTLVKRYPEPKMWVLGNLREPQIIADLL-----
+>23892|Ga0310888_10269646_2|-120|01	75	0.292	4.209E-13	4	85	91	189	270	280
+----ELVLYVSSASPASTQARRNLELVLDGFDRSQIKYTICDLGRDPMAGEIDRVAFTPTLVKRYPEPRMWLLGTLRETDLVADLL-----
+>SRR3954471_22207506	105	0.388	1.455E-23	3	87	91	77	161	171
+---FRFRLYVAGTTPNSVQARANLSALCRRHLPGRYKIEIVDVSKQPDRALIEGIFMTPSLMKISPSPTRMIVGTLSPSDALMRALGL---
+>SRR5581483_9829629	105	0.383	1.455E-23	3	88	91	11	96	111
+---YSFRLYLAGGTARAMQAEAQLRHLCETRLPEGFELEVLDVTDHPDRAEEDRVLVTPTVIRLSPPPARRVLGSLSDEHRVGLALGLP--
+>Cyp2metagenome_2_1107375.scaffolds.fasta_scaffold1445432_1	88	0.363	1.652E-17	0	87	91	148	235	244
+RPRVQLVLYVDAVWVTSVRAQENLEKVLAGFDRSQVHLRICDVAREPLDAEKDQIVFTPTLVKRSPAPRAWVVGDLSDHDVVTALLEM---
+>UPI00034656C1	83	0.383	7.416E-16	2	87	91	153	238	252
+--KLELALYVTMPWPSSLRAKANLSRVLARVPEGHVRLAVCDLAREPERAEIDNVLFSPTLVKVWPAPKMWILGDLSEANVLTDLLSL---
+>UniRef100_A0A2V7Y706	73	0.413	2.912E-12	2	76	91	165	239	260
+--KIELALYVTLPWPSSLRAQSNLSRVLSGVPDGEVRLSVCDLAREPERAERDNVLFSPTLVKVWPEPKMWILGDLS--------------
+>SRR3954454_15902270	80	0.309	6.824E-15	2	85	91	154	237	247
+--RVELVLYVSSASAASVQARRNLEQVLERFERSQIKCSVCDLVRDPLAGTDDRVAFTPTLVKRFPEPRMWVIGNFRDPEVVADLL-----
+>SRR5438045_7802661	70	0.270	2.599E-11	0	84	91	42	125	126
+RRKVELVLYTSAASEKCQRAIRSIQQVLERYERDQVSFTICDLCCDPEAGDADAVIFTPTLVKRGAEPKRWIVGSL-DRPRLGAG------
+>AntDryMetagUQ889_1029465.scaffolds.fasta_scaffold07537_1	79	0.297	1.287E-14	2	85	91	66	149	159
+--RVELVLYVSSASPASVQARRNLERLLAGFDGSQVKFTVCDLVRDPTAGDSDRVAFTPTLVKRYPEPRMWVLGNLREPQIVADML-----
+>SRR5678816_3727367	82	0.333	1.920E-15	0	86	91	82	168	182
+RPPIELVLYVSSLSPHSIAALRNLRQTLAQYGGGAVKLTVCDLSKDPSLADRDGVHFTPSLVTTGHGPRTWIVGHLGNPQVLQAFLE----
+>18084|scaffold527937_2|-1158|01	84	0.380	3.934E-16	2	85	91	186	269	284
+--KVELALYVTLPWPSSLRAQTNLSRVLARVPEGEVRLSVCDLARDPGRAERDNVLFSPTLVKVWPEPKMWILGDLTETEALADLL-----
+>MGYP000592549691	85	0.345	1.520E-16	2	85	91	141	224	234
+--KIELVLYVSAASPASMQAQGNMERVLASFNRDEVAYSVCDLQQNPETADHDRVVFTPTLVKRHPSPRLWIIGDLRDGDIVADLL-----
+>BarGraIncu01122A_1022018.scaffolds.fasta_scaffold128416_1	83	0.290	7.416E-16	2	87	91	153	238	246
+--KVELVLYVSSASPASLQARRNLEQVLSRFAAGQVRWTIRDLGREPLAGEDDRIAFTPTLVKRFPEPRMWVLGNLRDTDILADMLRI---
+>3300006028.a:Ga0070717_10004647_5	86	0.395	8.062E-17	5	85	91	13	93	103
+-----LRLYVAGDAPNSVAALVHLRAALAELPADRVDLEIIDVLQEPERGLRDDVLMTPMLVRHRPAPERRVLGNLGAARALRNVL-----
+>SRR6185436_10889035	74	0.400	1.090E-12	5	76	91	30	104	105
+-----LRLYVTGHTSSADRARAALRDVerrLAQQGEAQVIAEVIDVLDDPESAGRDQVFATPTLIRLTPAPQIRLFGDLS--------------
+>SRR5438132_14018022	99	0.386	1.685E-21	0	87	91	7	94	105
+RESFVVRLYVADREATAVRAIANLEALCREFLPRGCELEIIDILREPQRGLDDSIMVTPTLINLAPQPVRRICGDLGHPGRLRDGLGL---
+>SRR5438552_3472945	79	0.369	1.287E-14	2	85	91	378	461	478
+--KVELALYVTLPWPSSLRARSNPPRVLNGVPEGEVRLDVCDLAREPDRAERDNVLFSPTLVKVWPEPKLWILGDLSEPAVLTDLL-----
+>GraSoiStandDraft_28_1057319.scaffolds.fasta_scaffold3853791_1	78	0.306	3.331E-14	0	87	91	124	211	219
+RHKVELVLYVSSASPASIQARRNLEMLLSRFATNQVQWSVRDLGRDPLAGVEDRITFTPTLVKRFPEPRMWVLGNLRETDLLADMLRL---
+>SRR5688572_14330442	104	0.411	3.763E-23	3	87	91	54	138	144
+---FKLRLYVAGNTPNSAQARTNLRALCRTHLSGRHEIEIVDVTREPNRALTDGIYMTPSLLKLAPSPVRMIVGTLSHPESLMDALGL---
+>UniRef100_A0A512HC53	79	0.382	1.327E-14	5	85	91	3	83	91
+-----LDLYLAGRSRNSMRALHNLKEWLAQYGGEGVELRVIDVLEHPDRALDEGVLVTPTVIRREPDPIRIVVGTLDLPDDVTVLL-----
+>SRR5687768_18073040	77	0.329	8.623E-14	4	85	91	30	110	117
+----QLVLYATAGSTSSSRARRNLEAVLERFDPATYELAICDPSVEPLRAEDDRVVFAPTLVRRGPQPG-WFLGDLSNTAALHDML-----
+>SRR5215510_1611124	79	0.297	2.426E-14	2	85	91	156	239	241
+--RVEFVLYVSASSPASGQARRNLEQLLDRFDAAQVKYAICDLGRDPMAGEHDRVAFTPTLVKRYPPPRMWLIGSLRETETIADIL-----
+>SRR4029453_630509	82	0.329	1.920E-15	0	90	91	63	153	157
+KPSIELVLYVSSDSPHSVAALRNLRRTLAQYAGDAVRLTVCDLSKDPSLAERDGVHFTPSLVTAGRGPRTWIVGHLGNPQVLQAFLESALE
+>SRR5678816_1688620	78	0.325	4.573E-14	2	87	91	85	170	177
+--RVELVLYISPASFPSRAAERELRTILSQYDAGRVSLRIADVSRETSDAARDHVIFTPTLVKRRPEPLVWVVGDLTHVEVVHDLLQL---
+>SRR5262252_1015889	79	0.311	1.287E-14	1	90	91	52	141	145
+-PSIELVLYVSSVSPHSMAALRNLRRTLAQYGGDAVRLTVCDLSKDPSLADRDGVHFTPSLVTTGHGPRTWIVGHLGNPQVLQAFLESALE
+>SRR5687768_1317515	79	0.309	1.287E-14	2	85	91	203	286	295
+--KIELILYVSSESPASLQALRNLDRALARFDASQVKLTVHDLARTPEAGAADRVAFSPTLVKTFPEPRMWVIGNLRDPEVLEDLL-----
+>UniRef100_A0A958FYA2	80	0.382	9.667E-15	2	82	91	1	81	90
+--KISLRLYYTGGSPVSELARVALKALQSRHSSVQFEIEEIDVVLYPDAAEADGILATPTVIKFSPLPIAKIVGDISSLEQVL--------
+>MGYP001204866127	86	0.357	5.872E-17	4	87	91	50	133	142
+----HLRLFVTGGTSLSAAAVARLKELEEKLPADFLSMEIVDVLEDPDSAENNRVLATPTLIRMSPLPMIRVVGDVESVDRLMQLLDL---
+>SRR6185503_18435269	106	0.418	5.624E-24	4	89	91	38	123	130
+----QLRLYVAGDSPRSEQAIRSIRRLDGTRLAGRYDLEVIDVLTQPERAESDHVLATPTLLRLSPGPCRRILGDLGDLDRLITALVPPP-
+>UniRef100_A0A964QAK1	95	0.414	4.135E-20	4	85	91	7	88	99
+----QLRLYVAGDSPRSQLAIRSLRRLDGTPLAGRYDLEVVDVHDQPYRAEVDHVLATPTLLRLSPGPCRRILGDMGDLDLLMRNL-----
+>SRR3954462_9021407	91	0.455	1.797E-18	3	90	91	18	107	115
+---YALRLYVAGGTKHDGAAVRAVERLRERLGakGATIELEVIDVLAAPDRAEQDRILATPTLVRVTPQPARKIVGDLGDVERVSRMLDLGPE
+>MGYP001286529226	86	0.373	5.872E-17	5	87	91	9	91	108
+-----LRLFVTGGSLYSRRALVTIAELTRRMPDLHCEGEVIDLLEQPERAGLERIMATPTLIRLEPEPARRIVGDLRDADSLMSVLEL---
+>SRR5688572_18846285	104	0.436	2.741E-23	4	90	91	42	127	133
+----QLRLYVAGASPRSEQAIKHLQRLDGTELAGRYDLEVVDVFREPDRAEADRVMATPTLLRIAPGPCRRILGDLGDLDALLRALG-PAE
+>SRR5580700_4777658	89	0.452	6.385E-18	3	75	91	46	118	119
+---YQLTLFVSGASELSARAIVDARRLCEMGGPGRYQLVVVDVHDEPDAALANDILATPTLIKHRPLPVRRLVGDL---------------
+>UniRef100_K9VYB0	94	0.421	1.070E-19	3	85	91	53	135	141
+---YIFRLFVSGHNLDTERTLQILHRLLEQSLGHPYTLKVIDIFKHPEQAEANSISATPTLIRISPQPIKRIVGELDDVERVLKLL-----
+>14399|Ga0335069_12962689_1|-154|01	106	0.528	1.060E-23	1	89	91	8	96	109
+-EHLSLKLYVAGHTARSECAVAQARRLSELQFGGRCTLEIVDVVENPEIAEEERILATPTLVKVSPPPVRRIIGDLTRLDDVLAGLGLLP-
+>A0A1Z4NNA9	93	0.518	1.955E-19	3	85	91	166	248	256
+---YVLRLFIAGHTLHTERILQTLHELLEKHLSHPYTLKVVDVLTHPDQAEINQVSATPTLVKVFPPPMRRIIGNLESAERILQML-----
+>UniRef100_A0A1Z4NNA9	95	0.518	5.677E-20	3	85	91	166	248	256
+---YVLRLFIAGHTLHTERILQTLHELLEKHLSHPYTLKVVDVLTHPDQAEINQVSATPTLVKVFPPPMRRIIGNLESAERILQML-----
+>UniRef100_A0A0V7ZRD6	98	0.505	6.176E-21	4	86	91	166	248	256
+----VFRLFIAGHNPATEHILQTLHEILEKYLGHPYTLKVIDVLSHPEQAEANQVTATPTLVKVWPHPIRRIVGDLNNIGKILQNLG----
+>UniRef100_K9TAR4	93	0.493	2.017E-19	3	83	91	162	242	247
+---YVLRLFVAGNDLTTKRTLETLHQVLEQQLQHPYTLKVIDILKHPELAETNQVSATPTLVRVWPRPVRRIVGELEDLQRAIQ-------
+>SRR5262249_2417559	100	0.447	6.515E-22	3	87	91	15	99	103
+---YRFHLYVAGASMQSRRAIMRINEIGRRYLDGSYELQVVDILQNPEKVAEAGVVATPTLIKTSPPPVRYFVGDLSDTKKIVTGLAI---
+>UniRef100_UPI002021B723	99	0.518	2.387E-21	3	85	91	209	291	297
+---YVLRLFVAGHSATTERILQTLHQLLEQYLHHAYTLKVIDVFKHPEQAEADQVSATPTLVKVWPQPIRRLVGELDNLEKLLQIL-----
+>HubBroStandDraft_5_1064220.scaffolds.fasta_scaffold442189_2	95	0.505	5.502E-20	3	85	91	192	274	280
+---YVLRLFVSGSNPNTEHTLVTVHQLLEQSLNHPYTLKVIDVFKHPEQAESDQISATPTLIKIWPKPVRRIVGELNDAEKIRRLL-----
+>S7VCH7	116	0.523	2.810E-27	3	86	91	1	84	90
+---YSLTLFITGNGPASARAEQNLRRICDHAMDGQVRLEIVDVLQSPELAEEEGILATPTLIKRAPPPIRRLIGDLSDEAQVLAGLD----
+>UniRef100_S7VCH7	113	0.523	1.939E-26	3	86	91	1	84	90
+---YSLTLFITGNGPASARAEQNLRRICDHAMDGQVRLEIVDVLQSPELAEEEGILATPTLIKRAPPPIRRLIGDLSDEAQVLAGLD----
+>CoawatStandDraft_6_1074263.scaffolds.fasta_scaffold645439_1	82	0.439	1.398E-15	4	85	91	165	246	250
+----VLRLFVSGHSAMTEQILTTLQGVLESSRYQPYTLQMVDVSKHPEQAEADQVAATPTLVRVSPRPVRRLVGDLDNPRAILSLL-----
+>UniRef100_UPI00045E5B31	63	0.308	8.088E-09	5	84	91	137	217	233
+-----LTLYVVSLNSETRRLVEQITvALAKLYDPGHWVLDVVEVLGMPEKALEKDVFATPMLVRDVPEPVLKLLGDLSRVPSVIAA------
+>UniRef100_A0A969VCT1	102	0.530	2.598E-22	3	85	91	181	263	269
+---YVLRLYVSGSNPSTERTLVTIHQLLEQSLHHPYTLKVIDVFKHPEQAEEDQISATPTLIKIWPKPVRRIVGELNDAEKIMRLL-----
+>UniRef100_A0A517P709	83	0.341	5.573E-16	3	81	91	19	97	153
+---YRFQLFVTGNSLLSRRAREHVERHLVGPLGNRAEVEIVDLIADPIAARRERIVATPTLIRLEPSPVVRLIGDLTDFDRV---------
+>SRR3954454_16723622	97	0.457	8.218E-21	5	87	91	31	112	116
+-----LTLYVVRGTPASERAIATIEQLRAA-LPGTVKIEVIDVADQPEVAETERIVATPMLVRVAPAPVRRIVGDLSDLDRVRWGLGL---
+>SRR5581483_7974966	86	0.344	5.872E-17	1	87	91	35	121	126
+-PPLHLRLFVSGSSTTSLHARAAVDRLQRDGFVVAESVEIVDVLAEPERAAADRVLVTPTLLRVAPAPSRRVLGDLSDLAAVARALGL---
+>SRR4030095_4975553	78	0.320	4.573E-14	5	85	91	40	120	126
+-----LVLYISANSRYASVARCNCQRLLDRFDPRQVRFEVCDIGAHPERAEEDSVCYTPMLVKRHPLPRAYVLGDLSNGEPLVHLL-----
+>UniRef100_D0LT55	65	0.315	1.206E-09	0	87	91	141	232	237
+KERVELCLYLHSGTSASAAAETNLQNALADFDTTRLQLESIDLARSPRAAhPEDKVVFTPTLVRRGPGSRLALVGDLGDRallDSVLLAAGL---
+>22902|Ga0257122_1006421_4|-3359|00	108	0.510	1.154E-24	2	85	91	1	84	99
+--KYYLTLYVTGETPNSQRAIANLEKLSEECDADEFDIQIIDLLKHPDLAAEDEIIAVPTLVKKLPKPMQKIVGDLSNCEEVLLGL-----
+>UniRef100_A0A6L9ZJT6	103	0.530	1.004E-22	3	85	91	168	250	262
+---YVLRLFVSGNSIGTERAMKSLHQILEQSLSHPYTLKVIDVLQHPEQAEADQITATPTLIRVWPLPVRRIVGEFNDVEKILTLL-----
+>A0A0M1JMY3	85	0.421	1.520E-16	3	85	91	182	264	269
+---YVFHLFVSGRSAITQRTMEILHQILEDSLGMTYTLKVIDISRHPEQTEIYQITATPTLVKIWPLPMRKIVGDLENLDKLRQVL-----
+>UniRef100_UPI001E41D742	88	0.421	1.705E-17	3	85	91	178	260	265
+---YVFHLFVSGRSAITQRTMEILHQILEDSLGMTYTLKVIDISRHPEQTEIYQITATPTLVKIWPLPMRKIVGDLENLDKLRQVL-----
+>SRR6185503_14955318	93	0.400	1.955E-19	3	87	91	17	101	116
+---YEFDLFVVGGSEKAKRAEENLRRLGDEVLGGAYELRIIDVLENGEAAEAANIVATPALVRRAPLPVRMIVGDLSEPTWLAHGLGL---
+>SRR6185312_15773949	92	0.407	5.058E-19	2	82	91	38	118	119
+--KYELELFVVGGSVKAQRAEQNLRRLCDALLAGRYELRITDVLDNADAAEEANIVATPALLRRAPLPVRMVVGDLSERDALL--------
+>SRR6476619_1219783	96	0.411	1.549E-20	3	87	91	58	142	149
+---YKLELFVVGGSVKAQRAEQNLRRLCDSALAGRYELRITDVLDNADAAEEANIVATPALVRRAPLPVRMVVGDLSERDALAYGLGL---
+>SRR5690606_38631219	98	0.395	5.986E-21	2	87	91	31	116	122
+--KYQLELFVVGHSSKAQRAEHNLRRLCDARIAGRYELQITDVLENADAAEAANIVATPALVRRAPLPVRMVVGDLSERSALVYGLGL---
+>SRR5688572_23129716	89	0.360	4.650E-18	2	87	91	11	96	109
+--KYDLELFVVGGSARGRHAEDNLRRLCDASIAGHYTLRVTDVLENAEAADAANVIATPAVLRHSPLPRRMIVGDLSRRDALVHGLGL---
+>SRR5688572_20232672	92	0.412	5.058E-19	2	81	91	74	153	154
+--KYELELFVVGHSSMARRAEHNLRRLCDQTIAGRYELRVTDVLENAEAAEAANIIATPTLVRRAPLPVRMVVGDLSRRDAL---------
+>SRR6187401_742178	94	0.400	1.037E-19	3	87	91	21	105	118
+---FQLELFVVGRSAKAQKAEQNLRRLCEAKLAGRYELRVTDVLENADAAEAANIVATPALVRRAPLPVRMVVGDLSERSALAYGLGL---
+>SRR5687768_3218532	105	0.395	1.997E-23	0	90	91	9	99	111
+RPTYQLELFVVGHSAKAQRAEHNLRRLCDEKLAGQYELRITDVLENADRAEAANVVATPALIRRAPLPVRMVVGDLSERDALAYGLGLEAE
+>SRR6187401_2120497	94	0.383	7.553E-20	2	87	91	11	96	111
+--KYELELFVVGHSSKAQKSKHNLRRLCEAMLAGRYELRVTDVLENADAAEAANIVATPALVRRAPLPIRMVVGDLSERKALVFGLGL---
+>SRR5262245_48067131	116	0.445	2.047E-27	5	87	91	16	98	109
+-----LRLYMTGTRSRSVRALENIRKICEEFLPGEYELEVVDLYQQPEKAAQEQIVAAPTLVKYYPLPARRVIGDMSDSDRVLHGLEL---
+>UniRef100_A0A934ZFS7	93	0.383	2.017E-19	2	87	91	3	88	103
+--RYQLELFVVAHSTKAQRAEHNLRRLCDAKLAGQYDLRITDVLEDAAAAEAANIVATPSLVRRAPLPVRVVVGDLSRRESLLYALGL---
+>UniRef100_A0A852ZR84	106	0.390	1.093E-23	2	88	91	35	121	136
+--PYVLTLFVFGPDESSRRAATHLRRLCDELVGGQYRLEVVDVGEDPELAEEFGIFVTPTVVRTQPLPQFRVIGDLSDDARTAAALGFP--
+>ERR1700733_14812660	121	0.482	4.579E-29	3	87	91	67	151	163
+---YRLRLIVAGRTTRSQRAIENLRRICDEHLGGQVDLEVIDIYQQPELAEKYQVIAAPTLIKLLPLPIRRVIGDLSEKERVLRGLEI---
+>MGYP001377341570	91	0.400	1.309E-18	3	87	91	3	87	96
+---YQFKLFVTGETVHAAAARATVDRLCDALQIEAHAVRIIDVLEEPDLAAADRIIATPTLIRTSPQPERRVIGDLSDLDTLLKTMRL---
+>SRR5690242_13802588	108	0.441	1.154E-24	5	90	91	20	105	118
+-----LELYVMGDSPKSRAALDNLRRICERRLAGRYDLQVIDVIEQPDAAEAANVVATPALIRRGPAPVVRVVGDLSDRTALVHALGLDAE
+>UniRef100_UPI001FB8EED0	112	0.404	5.013E-26	3	86	91	13	96	110
+---YQLRLFIAGTSPRSQRTIENLRRICREHLADRHSLVIVDIYQQPELAEAAQVVAAPTLLKLTPEPLRRIVGDLSDEARVLRGLG----
+>UniRef100_A0A521U920	100	0.441	6.722E-22	2	87	91	3	88	109
+--RYELELFVVGHSAKAERAESNLRRLCEARLAGRYDLRVTDVLEDAEAAEEANIIATPTLLRRAPLPVRMVVGDLSHRGALLRGLGL---
+>UniRef100_A0A933USY1	86	0.416	6.058E-17	4	87	91	8	90	97
+----VLRLYIAGHSPNSVVALANLDWLRRAHFQDA-TVDIVDILREPERAMADRVITAPALFKVAPSPPRMLLGNLSDATKVLQGLGL---
+>SRR5471030_2470326	96	0.480	1.549E-20	3	79	91	115	191	193
+---WQLRLYVVDQTVKAVTAYTNLKKIYESRLKGRYRITVIDLLKHPQLAKGDQILAIPTVVRKLPVPIRTIIGNLSDTD-----------
+>UniRef100_UPI001F066344	109	0.411	6.318E-25	3	87	91	14	98	109
+---FHLRLYVAGQTPRSALAQANLYALCEARLPGRHHIEIVDLMDEPARARTDGVIAVPTLIRVSPTPVRRVVGDLSDTARLLAGLEL---
+>14341|Ga0209698_10565634_1|+23|00	112	0.348	6.670E-26	1	89	91	33	121	134
+-PRYEFRLYIAGTNLNSVRAIENVRRLRKSLRPSRCKLEIIDLYQQPALAKRDQVVAAPALVKLYPLPRRTFVGDLSDSARVVAGLGIIT-
+>UniRef100_UPI001BEB70D6	108	0.465	1.191E-24	2	87	91	10	95	99
+--RYRLRLVIAGNSERSRRAIENLQHLCAEHLSGQVDLEVVDIYQRPELAEEYQVIAAPTLVKLLPLPVRRIIGDLSQEDRVLHGLEI---
+>SRR5215218_6711373	87	0.404	2.269E-17	4	87	91	12	95	102
+----VLRLYIAGNSSSSRRAEQNVMRLRDHMTADAWKIEIIDVLATPELAEQASILATPTLSYDNAGRPRRIVGDLSDTKRILDYLGI---
+>SRR6188768_3491203	81	0.366	2.636E-15	2	72	91	41	111	112
+--KYELELFVVGRSSKAQKAEHNLRRLCEARLAGRYELRITDVLENADAAEAANIVATPALVRRAPLPVRMVV------------------
+>SRR6187402_177262	96	0.470	2.126E-20	4	88	91	0	84	99
+----ELTLFVAGDTAKSALAATKLRHICESLARGNYTLAIVDVLKDSAAAEREKILVTPTLIKRSPPPTRRLLGDLTATAKVVETLGLP--
+>SRR6059058_1539947	101	0.404	4.746E-22	3	86	91	13	96	103
+---FHFRLYVAGDTPNSERARVNLGALCRKHLVGRYKIQIVDVFKDPNRAMIEGIFMTPTLIKVAPSPIRMVVGTLSQSAALMEALG----
+>SRR3984957_851882	121	0.476	3.336E-29	2	87	91	14	99	105
+--RWLLRLYVAGQSPKSLQAFANLMRIRDEHLGSEYEIEIVDLLENPQLAEGDEIVAIPTLVRRLPHPMRKIIGDLSDTDRVLVGLQL---
+>SRR3954470_18350552	106	0.447	5.624E-24	3	87	91	19	103	112
+---YQFRLYVAGDTPNSERARVNLGALCRKYLVGRYKIQIVDVFKDPDRAMVEGILMTPTLIKLAPSPVRMVVGTLSPSESLMDALGL---
+>MGYP001434196702	99	0.388	2.314E-21	3	87	91	34	118	123
+---YKFRLFVADDTLNSAQASVNLAALCRAHLPGRHEIEIVDVLLEPKRALAEGVFLTPTLIKFSPLPVRRIVGTLSEPLTVLRALGL---
+>UniRef100_A0A4V1DI91	40	0.289	5.717E-01	0	74	91	10	85	373
+QRRYLKLLLVAAPHHRATPDLRGLVAFLEnQDFGFDVSLEIADPAERPELLELHRLVATPALIKLDPTPKQVFAGN----------------
+>MGYP000867160857	47	0.297	3.483E-03	2	74	91	29	102	379
+--RYLKLLLVAAPHHRANPDLRGLVAFLENQDFGfDVTLEIADPAERPELLELHRLVATPALIKLEPTPKQVFAGN----------------
+>UniRef100_UPI002001187B	44	0.311	4.536E-02	0	75	91	9	85	372
+RRPHLKLLLVAGTRHRASADVRSLVAFLEKEDFGfEVSLELADPAQRPELLELHRLVATPALIKLEPAPKQVFAGNM---------------
+>MGYP001295239604	51	0.328	1.461E-04	7	75	91	50	119	406
+-------LLVAGTRHRASADVRSLVAFLEKEDFGfEVSLELADPAQRPELLELHRLVATPALIKLEPAPKQVFAGNM---------------
+>MGYP000025237920	48	0.358	9.797E-04	7	81	91	40	117	121
+-------LLVASPHHRATPDLRGLMAFLEHEDFGfDVQLDVVDPALRPELLELHRLVATPALIKLEPSPRQVFaeIGRASCRERV---------
+>UniRef100_A0A560LTW2	39	0.277	2.028E+00	5	74	91	17	88	381
+-----LTLLIVATSQHlSSPGLRGVLQFLESHDYGfELNLQIADPAKRPELLELYRLVATPAVVKLHPAPRQVFAGN----------------
+>UniRef100_A0A076H9F6	41	0.329	4.166E-01	7	84	91	14	90	383
+-------LLVAARHHLSGQDLRSLVQYLEREDVGfEVTLQLADPSQQPELLELHRLVVTPALIKLSPSPKQVFAG--SNIHQQLKG------
+>MGYP000311810024	47	0.306	2.536E-03	0	73	91	19	92	206
+RQPLKL-LLVAARHHLSGQDLRGLVQFLErEDLGFEVTLQVADPSQQPELLELHRLVVTPALIKLAPNPKQVFAG-----------------
+>UniRef100_A0A968YHU9	46	0.295	4.933E-03	4	73	91	22	91	390
+----QLLLFVDKRAT-AKEQIQKISQYLETlEPQCDFELHVVEVAEQPYLVEHYKLVATPALVKIRPEPRHILAG-----------------
+>UniRef100_UPI000B35C714	42	0.269	1.611E-01	1	74	91	13	88	385
+-ERQVLHLLLV--ATRQQLAGQDLRTLLqllrREDLGFEVSLEVADPRRQPELLELHRLLATPALVKLAPAPKQVFAGN----------------
+>MGYP001088475876	43	0.310	8.285E-02	1	73	91	12	84	189
+-KKLELIL-VAGRKHLSRKDISEMLKFLEsKECNFEVSIQLSDPTKQPELLELHRLVAIPALIKIFPEPKQIFAG-----------------
+>UniRef100_A0A7Y3TRI1	56	0.281	2.441E-06	4	73	91	15	84	443
+----QLLLFIDKR-PSSREQVQQVRMALKELReECDFELQIVDVSEQPYLAEYFRLIATPALVKLHPEPRQILAG-----------------
+>UniRef100_A0A352XFA5	54	0.309	8.682E-06	4	73	91	15	84	396
+----QLLLFIDER-PTSRKHIHRIRSYLETLRADyPFELMLISVGEHPYLAEHFRLVATPALIKIHPPPRQTLAG-----------------
+>UniRef100_A0A5J6Q9E6	42	0.306	2.211E-01	0	73	91	3	76	394
+RPELRLLL-VASKAHAASQDVRSMMALLEQDDCGfQVTLKLADPRQQPELLELHRLVATPALVKLLPLPRQTFVG-----------------
+>UniRef100_A0A139WUD4	60	0.328	7.451E-08	2	73	91	13	84	395
+--PLQLLLFVDGR-PKSRQQVQRILSYLEELQADcKFELQIVDVGQKPYLAEHFKLVATPALIKIHPEPRQILAG-----------------
+>UniRef100_A0A926Y835	54	0.352	8.682E-06	4	73	91	15	84	391
+----QLLLFV-DDRPSSRKQLQQIYSYLEQIKADNsFELQVVEVGEEPYLAEHFKIVATPALIKIHPAPRQALAG-----------------
+>UniRef100_UPI0020A7ECEA	56	0.295	2.441E-06	4	73	91	15	84	397
+----QLLLFIDER-PSSRKHIHRIRSYLETLRADyPFELMVVSVGEHPYLAEHFRLVATPALIKIHPLPRQTLAG-----------------
+>UniRef100_A0A068MZV1	50	0.260	2.071E-04	2	73	91	13	84	383
+--PLQFLLFI-DDRPSSQDSVQEISQCLGTLVDGHsYDLQILQISKHPHLVEHFRLVATPSLIKLQPEPRQVLAG-----------------
+>UniRef100_UPI0016873C22	61	0.352	3.951E-08	4	73	91	16	85	382
+----QLLLFV-DQRPSSQEHIRQVRQFLEELNaQDEFELQIIDVGEQPYLAEHFKLIATPTLIKIHPEPRQVLAG-----------------
+>UniRef100_A0A0M1JTJ0	57	0.347	9.426E-07	4	74	91	15	85	394
+----QLLLFVDSR-PHSAEQIQEIRNYLKQWRTEfPYNLEIINVVEEPYLAEHYKLIATPTLLKLYPEPRQVLTGN----------------
+>UniRef100_A0A261KTE7	53	0.309	3.088E-05	4	73	91	30	99	405
+----QLLLFINKR-PGSQEQIQAIRKSLSKLKTDyPFEFNVIDVGEQPYMAEHFKLIATPALLKIHPEPRQTLTG-----------------
+>UniRef100_A0A3C0N9T6	52	0.323	4.240E-05	4	73	91	15	84	398
+----QLLLFVDER-LSSRKHIQRIRNYLKTLRIDyPFELMVVDVGEQPYLAEHFKLVATPALIKIHPKPRQILAG-----------------
+>UniRef100_A0A6J4I559	51	0.323	1.098E-04	4	73	91	15	84	398
+----QLLLFVDER-LSSQKDLQQISSYLETLRAEyPFELMIVDVGEHPYLAEHFKLVATPSLIKIHPKPRQILAG-----------------
+>UniRef100_A0A6P0QL46	59	0.352	1.930E-07	4	73	91	15	84	397
+----QLLLFVDER-PSSRKHIQRIRSYLETLKADyPFELTVVDVGEQPYLAEHFKLVATPALIKIHPNPRQTIAG-----------------
+>UniRef100_A0A846D500	52	0.309	4.240E-05	4	73	91	21	90	392
+----QLLLFVDER-PSSQENIQQIHSYLESLKADyPFELQVIEIAEQPHLVEHFRLLATPALVKIFPAPRQTLAG-----------------
+>UniRef100_A0A350Y740	56	0.309	2.441E-06	4	73	91	15	84	386
+----QLLLF-TDERPSSRKHIHRVRSYLETLRANyPFELRIVDVGEQPYLAEHFKVVATPALIKIHPLPRQTLAG-----------------
+>UniRef100_A0YTH4	56	0.380	1.778E-06	4	73	91	15	84	394
+----QLLLF-ADQRPSSKEQIGEIRQFLEKLNcEEAYELQVIDVGQQPYLAEYFKLVATPALVKIFPEPRHILAG-----------------
+>UniRef100_L8M9H5	47	0.295	2.617E-03	4	73	91	33	102	405
+----QLLLFVDKR-PGYRKKIQRVQAYLDDLkLEQDFQLEVIEIDKQPHLVEYFKLVATPALVKISPQPRQVLAG-----------------
+>UniRef100_UPI001D02CB6E	63	0.315	5.889E-09	2	73	91	13	84	395
+--PLHLLLFVDGR-PKSRQQVQRIRAYLKELQAEySFELEIIDVGQQPYLAEHFRLVATPALIKIHPEPRQILAG-----------------
+>UniRef100_A0A6P0XB78	55	0.338	4.604E-06	4	73	91	15	84	390
+----QLLLF-TDERLSSRKTIQQIRHYLESlRMEYPFELKVVDVGKQPDLAEHFKLVATPALIKIHPQPRQTLAG-----------------
+>UniRef100_A0A6M0G3C9	53	0.338	3.088E-05	4	73	91	15	84	390
+----QLLLF-TDERLSSRKNIQKIRHYLESLkMEYPFELKVVDVGKQPDLAEHFKLVATPALIKIHPQPRQVLTG-----------------
+>UniRef100_A0A937N7H3	72	0.290	3.998E-12	2	87	91	2	87	396
+--KFTLTLYIVGKGTDWASVVERLEAICKTDLAGHYGIESVDVANGQDLSGDMRILAPDAVMLWLPAPLQAPMNDLVNAKPGLVGLDL---
+>23040|scaffold_1553752_c1_1|+3|10	100	0.448	1.228E-21	1	87	91	18	104	123
+-PRFKFSVYIAGQTRQSELALARLRKICDEEIPANYEIEIIDLAKNPHLAKEHQILATPSIFRTLPAPVRKSIGNLSKADKTLLGLDL---
+>ERR1700732_1595525	94	0.402	1.037E-19	0	86	91	15	101	108
+KPRFKFRVYIARPTRKSDLALARLRALCAEAFPDDYDIEMIDLAKSPHLAKEHQILATPAVFRTLPAPVRKSIGDLSKTDKSRLGLD----
+>SRR5882762_5740304	94	0.397	1.424E-19	0	87	91	36	123	136
+KAQFKFQVYIARPTRQSDLALARLRAICDEAIPDDYDIEIIDLAKRPGLAGKFQIVATPTILRTLPAPIRKSIGDLSKTDKALLGLDL---
+>SRR4030081_2822547	95	0.420	4.008E-20	1	88	91	31	118	121
+-PRYKFSVYIASPTRASESALARLRKICEEQIPNEYEIEVFHLSKNPQLARDHNIIATPAIFRTLPAPVLKSIGDLSRTDQALLGLDLL--
+>SRR6185295_12007002	113	0.471	1.879E-26	1	89	91	177	264	270
+-PGWVLRLYVAGMNRTSARAVERVHAICDEYLAGRYELEVIDIYQLPALARGHQIVATPTLIRLLPAPLRRYIGDLSN-ENLVFGLDLKP-
+>SRR5215831_4900645	114	0.558	9.973E-27	1	86	91	71	156	163
+-PGLVLRLFICGTSPRAASAVKNLRFICESELHGAYSLEIIDVLEQPDLAEEAKVLATPTLIKLLPLPLRRIIGDLSDKEKLLIGLE----
+>SRR5712672_2296523	88	0.409	1.652E-17	0	87	91	11	98	106
+KTRFKFSLCIARVTEKSKAALARLRAICDETIPKKYDIKVIDLSKNPELARDHNIIATPAVFRTLPTPVRRSIADLSRNDRALLGLNL---
+>12123|Ga0209625_1018534_1|-121|00	95	0.415	5.502E-20	1	89	91	15	103	115
+-PNFRFQLYIGGPTRASDEVLGRLQAICDEAIPNDYAIEIIDLSKNPQLAKDHQIIATPSVFRTLPEPMRKSIGDLSLKQRTIIGLDLLT-
+>SRR4051812_48803690	106	0.404	5.624E-24	1	84	91	45	128	135
+-EHYSLALYITGSSPRSALAISAIRKICDTHLLGCYSLEIIDLTQQPLRARSEQIVATPTLIRRLPFPIRRFIGDMSLVERQLLG------
+>UniRef100_A0A932SCN4	121	0.579	6.484E-29	0	87	91	15	102	111
+QKVWTLRLYVAGQTPKSVTALSNLERICEAHLEGKYRIEVVDLLKSPQLARGDQIIATPTLVRRLPPPVKKIIGDLSNADRVLVGLDL---
+>SRR5579871_1513814	128	0.540	2.104E-31	1	87	91	59	145	153
+-ETWNLRLYVAGQSPKSLTAFSNLKRICETYLPGKYHIEVLDLLKNPQLAEGDQVVAIPTLVRRLPEPLRKIIGDLSNTERTLVGLDL---
+>UniRef100_A0A517YME2	122	0.505	2.508E-29	1	87	91	17	103	120
+-ETWELRLYIAGQTPKSVAAFRNLKKLCEEHLPGRYQIEVIDLMQHPQLAAGDQIVAIPTLVRRLPEPLRRIVGDLSNTERTLVGLQL---
+>ERR1035437_1623514	104	0.453	2.741E-23	2	87	91	28	113	122
+--KFVLRLFVAGATPRSRHAVRRVRELCETELKGNCELEVIDIYQQPGLARENQIVATPTLIIAFPPPLRRFIGNRTNITGLFVELDL---
+>SRR4030081_12347	93	0.397	3.684E-19	0	87	91	29	116	123
+KTRFKFAVYIARPSAESDAALARLRKICDETIPKNYDIRVIDLSKNPELARDHQIVATPAVFRTLPTTVRRTIGDLSNNDRALLGLNL---
+>UniRef100_A0A062V6S3	115	0.505	7.496E-27	1	87	91	18	104	111
+-EVWELRLYIAGQTARSDAALANLKRICEEHLAGKYRIEVIDLLKNPQIARDHQILATPTVIRKLPEPLKKTIGDLSQTERVLVGLDL---
+>ERR1700676_5527870	97	0.393	1.128E-20	1	89	91	58	146	156
+-PHFKFQLYIGRSTRASDAAITRLQAICDETIPDDYAIEIIDLSKNPQLAKDHQIIATPSVFRTLPEPIRKSIGDLSLKHKAIVGLDLPT-
+>15488|Ga0208981_1210027_1|+2|10	90	0.363	3.387E-18	0	87	91	24	111	119
+KTRFKFAVYIARPSAESDAALARLRKICDETIPKNYDIRVIDLSKNPELARDHQIVATPAVFRTLPTPVRKSVGELSSKDRTLLGLNL---
+>SRR5258708_39684100	96	0.400	1.549E-20	0	89	91	38	127	150
+KAHFEFCVYIANHTLRSDLALKRLKKICEENVPGDYEIEVVDIAKPPDIAKDRQIVATPAVFRTLPAPFRRLIGDLPHEERSLLGLDLFT-
+>MGYP000303408308	113	0.574	3.540E-26	1	87	91	1	87	102
+-KKFELKLYVTGQTARTETAMGNLKDLFDKELAEQYDLEVIDVLERPQLAEDERILATPTLIRKLPVPIRRIVGDLSNREQVLLGLDL---
+>SRR5258708_26871038	87	0.363	3.115E-17	0	87	91	32	119	129
+KHRFKFQVFIGKPSQKSDLAVARLREVCEAEIPGEYDIEIIDLSRTPELAGENNIVATPAVFRTLPAPVRKSIGDLVEKHKVLLALDL---
+>ERR1044072_7554525	109	0.529	8.406E-25	2	86	91	123	207	214
+--RYILKLYVTGRTSRAERAIANLRRLCEDELEGCYQLEGIDIVEHPQLAEDERVRATPTLVKQLPPPLRRGVGDLSSRAKGLFGLD----
+>18065|scaffold45210_3|+2239|01	117	0.465	1.491E-27	0	87	91	45	132	140
+RPYWNLRLYVAGSSPRSLAAVTNLTKVWEEHLPGPYSIEVVDLLEHPNLARADQILATPARVGALPSPIRRVIGDLSSRDRVLVGLEI---
+>SRR5262249_21381219	117	0.534	1.491E-27	1	86	91	16	101	117
+-EPFVLKLFICGASPRANSAVANLRHICEHDLQGHFTLEIIDVLEQPDLAEESKVLATPTLIKLLPPPLRRIIGDLSDKQKLLVGLD----
+>SRR4051812_10739495	120	0.593	1.184E-28	2	87	91	18	102	110
+--KFVLKLYIAGSSPRSQRAIANLHRICAEELPGS-EVDVIDVLQQPHLAEGARIMATPTLIKELPPPVRRIIGDLSDAEQVLLGLDL---
+>SRR6266404_266030	86	0.417	8.062E-17	9	87	91	0	78	113
+---------IASPTRESQLALTRLRKICDEQIPNEYEIEVFDLRKHPELATRYDIVATPAICRTLPAPLRKSVGDLSKTEKALLGLDL---
+>SRR5260370_32467315	102	0.422	1.834E-22	0	89	91	34	123	146
+KPHYKFSVYIANHTLRSNSALERLKKICEENVPGDYEIEVIDIAKSPGLATDHQIVATPAVFRTLPAPLRKSIGDLSQKDKALLGLDLFT-
+>UniRef100_V4JFK2	110	0.529	3.353E-25	3	87	91	3	87	94
+---YLLRLYIVGSTLQSERAIRNLRSICNKALHNRYRLEIIDVIEHPEAAQDAHIIATPTLIKELPPPLMRIIGDMSNQEKVLVGLDL---
+>SRR5258708_27969647	100	0.448	1.228E-21	3	89	91	14	100	104
+---FKLRVYIGGEALESDRAVARLRKICDEAAPNDYEIEVVDLSKNPQLASRYQIVATPTVIRTLPSPVRKTIGHMSKREKVLLGLDLVP-
+>UniRef100_UPI001565DCEC	109	0.482	8.673E-25	1	87	91	15	101	115
+-EVWELRLYVAGQTARSMTAFANLKRIAEQHLRGRYRIEVIDLKADPQRADEDGILAMPTVVCKLPPPLRKVVGDLSDTEKALVGLKL---
+>SRR5262245_2800084	119	0.541	1.625E-28	3	87	91	29	113	122
+---YVLRLYVAGMTARSMDAISRLKAICEEHLGEHYKLETIDIHQQPGLARDQQIVAAPTLIKELPPPVRRLVGDLTNRERVLVGLDL---
+>ERR1039458_2802747	93	0.413	3.684E-19	1	87	91	15	101	123
+-ESVELCLFVAGDAGPSARARRELEGLLVELGGGAWSIEVVDVLVRPDLAERARIVATPVLIRLAPLPRRSIIGDLSDWQVVAEVLEL---
+>ERR1700677_35954	83	0.416	5.401E-16	4	87	91	12	95	102
+----ELCLFVAGNTGPSARARRELEWLRVELEEGGWSIEGIDVTERPDLAERARILATPVLTRLAPLPRLSMIGDLSDWKVVAEVLEL---
+>SRR5450755_1004340	90	0.448	2.467E-18	4	90	91	126	212	214
+----ELCLFVAGEAGPSVRARRELDRLRMGLEGGGGRVDVIDVMERPDLAEQAGILATPVLIRLAPLPRRSIIGDLSDWEVVADVLELALE
+>MGYP000397992808	97	0.447	1.128E-20	3	87	91	3	87	95
+---FSFQLFVAGDTPRSHLAASNLRDLLDRVAPDDYDLEVIDVLERPDLAEKERILATPFVLKISPPPTRRVVGDLTDLALAARALDL---
+>SRR5437763_3353870	89	0.418	8.766E-18	2	87	91	37	122	129
+--PVELCLFVVGESGPSVRARRELEAFRVARGGDGWRVVVIDVLERPDVAERERILATPVLIRMAPLPRRGIIGDLSDWEAVAEVLEL---
+>SRR5450755_2832987	95	0.372	5.502E-20	2	87	91	20	105	112
+--RVSLRMYVASDTAPSADARRQLAALCERLGGERWEVEVVDVFERPALAEADRIVATPVLIRLFPAPRLSVIGDFSDLDAVAAALDL---
+>MGYP000274140882	87	0.419	2.269E-17	7	87	91	19	98	105
+-------LYVAGRSERSALAEQNLRAV-TQRLHGPVQIEVIDLTRRPDLAEELDIVATPMVLRVLPEPPRRVVGDLSDQALLAQALDL---
+>ERR1039458_272673	74	0.400	1.090E-12	14	88	91	24	97	113
+--------------PRSLTA-PQLERLRPELEGGGLGVEVVDVMQRPDLAERARILATPVLMRLAPLPRRSIIGDLSDWRLVTEVLELP--
+>ERR1051325_10531525	81	0.305	4.970E-15	1	85	91	28	112	122
+-PRIELVLYVTAASSHSAAATRNCEALLSRFDRRSVVFEICDISLHPERAEVDGICFTPVLMKRMPLPRAYVIGDLSNTAALVDLL-----
+>SRR5688500_6194403	81	0.329	3.619E-15	2	86	91	52	134	135
+--RLSLRLFVAGDSPDSETAIANLEALFPN--GSEAEIEIVDIQREPARAARESIMLTPTLLKLAPSPACRILGNLKNRDALLELLG----
+>SRR5438477_4004941	90	0.360	2.467E-18	0	85	91	467	552	558
+RERVALRLYVSPASPPSVKARRNMEKLLERIGPVNVDFEVLDLALEPLRAETDNVVFTPTLVKHWPEPRVWILGDLSDPVVVGDLL-----
+>SRR3954468_23038545	80	0.409	9.369E-15	5	87	91	32	114	129
+-----LRLYVAGEGPNSARARANLQRLLADVDSSRYVLEVVDCLDEPLRALSDGVPPPQTLMPVTPPPQRTIVGSLSAMDHVADALEI---
+>SRR5271165_1490500	102	0.383	1.336E-22	2	87	91	3	88	112
+--KFKFRIYVAGDALNSAQALANLDAICREYLPDRHEIEVVDVFREPKRALTDGVFMTPTLVKVAPFPTRRIVGTLSQTRLVLQAVGL---
+>UniRef100_A0A142HLX2	109	0.482	4.603E-25	3	87	91	14	98	109
+---YELVLYVAGATPNSTRAVRNIKAICEEYLPGRYALRILDIYQQPELAQQAQLVALPTLVRLRPLPQRRLVGDLSNRPVVLSVLGL---
+>A0A142HLX2	117	0.482	1.087E-27	3	87	91	14	98	109
+---YELVLYVAGATPNSTRAVRNIKAICEEYLPGRYALRILDIYQQPELAQQAQLVALPTLVRLRPLPQRRLVGDLSNRPVVLSVLGL---
+>SRR5512142_3015464	83	0.397	7.416E-16	0	87	91	23	110	118
+RSEIRLCLYVAGNAPNSVAARANLSAALAALDNVSAAVEIVDVFERPDLAVQNEVYVTPMLLRLAPPPKCRIVGSLSDRDAIVNILDI---
+>SRR5215208_4287972	81	0.285	3.619E-15	2	85	91	19	102	112
+--PIELVLYISAASAHTAAARRNCEALLARFDQRRVHFEICDVSQHPDRADTDGICFTPVLMKKLPLPRTYVVGDLSNTTALVDLL-----
+>SRR3954469_15268427	97	0.441	1.128E-20	2	87	91	18	103	115
+--RLVLRLYVAGDAPNSALARANLKRLLDSLDRDQYALEIIDCLDEPLRALNDGVLVTPTLLRLSPEPGRTIVGSLSAIDHVADALDL---
+>SRR5450432_1605686	81	0.348	2.636E-15	2	87	91	153	238	249
+--KIEITLYLTLPWPSSARAQANLQRVLARVPDGHVRLNTCDLAQEPGRAEKDNVLFSPTLVKVWPAPKMWILGDLSEAGVLTDLLEL---
+>SRR5688572_14714411	99	0.418	2.314E-21	2	87	91	50	135	150
+--PVLLRLYVAGDAPNSSRARANLRRLLADVDPAKYDLEIIDCLDEPLRALNDGVLVTPTLVRVQPEPQRTVVGTLSALDHVADALDI---
+>SRR5687768_10781478	82	0.305	1.398E-15	2	86	91	172	256	262
+--PIELVLYISAHSPRSAAAIENIKRVLARFSSSRVSLTICDLSLEPHKGEADSVAFTPTLVKRSPGPRTYILGHLANPEVLVELLD----
+>SRR5438105_8210914	83	0.302	7.416E-16	0	85	91	54	139	149
+KTRIELVLYVSAASSHTATARRNCEALLARFDQRRVRFEVCDISRHPDRAESDGICFTPVLMKKRPLPRAYVIGDLSNTAALMDLL-----
+>UniRef100_A0A2V8GSN4	79	0.302	2.503E-14	0	85	91	17	102	112
+KTRIELVLYVSAASSHTATARRNCEALLARFDQRRVRFEVCDISRHPDRAESDGICFTPVLMKKRPLPRAYVIGDLSNTAALMDLL-----
+>12689|scaffold1791547_1|-2|11	80	0.317	9.369E-15	1	85	91	85	169	187
+-PSVELVLYVSTASSYAASATRNCEALLARFDRRAVRLEICDVSEHPDRAETDGICFTPVLLKKQPLPRTYILGDLSNTAALVDLL-----
+>UniRef100_A0A948CES1	102	0.388	1.379E-22	3	87	91	56	140	151
+---YRFQLFVSGSSPRSTLARANLTKVCDETVPGNYTIEVVDVLLRPDLAEESSILATPLVVRVSPHPPRRAVGDFTDLERLAAAMGL---
+>SRR6185295_9098939	82	0.395	1.920E-15	2	87	91	339	424	440
+--RIELALYVTMPWPSSLRAKANLGRVLARVPEGLVHLNVCDLAREPLRAELDNVLFSPTLVKVWPAPKMWILGDLSEPEVLTDLLAL---
+>SRR4051812_7426335	87	0.392	3.115E-17	5	88	91	15	98	101
+-----LRLYVAGNAPNSTKARRNLDALLASFEPSSYQLEVIDCLSEAGRTLADGVIVTPTLVKFEPAPAATVIGTLSDADAVRAILRGP--

examples/data/KaiB_seq_ids.txt ADDED Viewed

	@@ -0,0 +1,364 @@

+101
+MGYP000886600007
+UniRef100_A0A971TK21
+ERR1044071_8151622
+UniRef100_A0A1F2SDQ0
+SRR5580692_7392111
+K9S6Z6
+SRR3954470_14000739
+UniRef100_UPI0018DCCDB9
+SRR5688500_3073545
+MGYP000149628109
+3740|scaffold08918_4|-3862|00
+SRR6187401_1816177
+SRR5512147_2964451
+26123|scaffold_438712_c1_1|+1|10
+12684|Ga0207652_11722284_1|+1|10
+SRR5580704_16544830
+UniRef100_A0A3N5XMK1
+UniRef100_A0A7W0IU42
+ERR1043166_4517172
+MGYP001057477004
+26195|Ga0315277_11393154_2|-158|01
+UniRef100_A0A8J7LTM0
+12918|scaffold901211_1|+1|10
+SRR5215510_15873647
+SRR5512142_679953
+UniRef100_UPI00190A34C5
+SRR4051812_34338196
+UniRef100_A0A3N5LPZ6
+UniRef100_A0A950DFQ4
+5937|scaffold842798_1|-17|01
+SRR4030095_15264891
+UniRef100_K9UBR2
+UniRef100_UPI001E525CD0
+UniRef100_A0A845X7U1
+3300017444.a:Ga0185300_10001144_2
+UniRef100_A0A349JMI1
+UniRef100_K9SDH8
+UniRef100_A0A3M1PH87
+UniRef100_A0A1C0V439
+UniRef100_UPI001E4DBDD3
+UniRef100_A0A969FMT9
+UniRef100_A0A0M2Q0K7
+UniRef100_A0A978SUS5
+UniRef100_A0A930TQ40
+UniRef100_A0A2W4XV74
+UniRef100_A0A939KSU8
+UniRef100_A0A8K1ZWU9
+UniRef100_UPI001C72C3C0
+UniRef100_A0A6P0TIA3
+UniRef100_A0A5B8NIC7
+UniRef100_UPI002012D1AC
+UniRef100_UPI001C0312E0
+UniRef100_A0A928Z921
+UniRef100_A0A351L2B1
+UniRef100_A0A8J7E209
+UniRef100_UPI00232B6744
+UniRef100_UPI0018EFA1D6
+UniRef100_U5DJK1
+UniRef100_A0A832M402
+UniRef100_A0A1C0VJA3
+UniRef100_A0A969K2I2
+UniRef100_K9X4T7
+UniRef100_W7Q8H4
+W7Q8H4
+A0A1H7MTT5
+UniRef100_A0A1H7MTT5
+A0A1P8R863
+UniRef100_A0A1P8R863
+UniRef100_UPI001CD124AC
+UniRef100_A0A2N5Y751
+UniRef100_A0A2N7UCY5
+MGYP001039280987
+UniRef100_U5T3B0
+UniRef100_A0A540VSD6
+UniRef100_A0A2S6G6T8
+UniRef100_UPI00124EBEF7
+UniRef100_UPI001439CAB5
+MGYP000294044467
+UniRef100_A0A4Q8CZA3
+3104|Ga0306908_1123748_1|-11|01
+UniRef100_UPI00133045FA
+UniRef100_UPI00201FFA50
+UniRef100_A0A845V233
+UniRef100_UPI00082AD250
+MGYP001134272031
+UniRef100_UPI00037CF37C
+UniRef100_UPI0003674D18
+UniRef100_UPI00047687D0
+UniRef100_A0A3S0W7L6
+UniRef100_UPI001903F48C
+SRR4051794_37995438
+UniRef100_A0A318VX16
+UniRef100_A0A372BWN2
+3300017992.a:Ga0180435_10008823_6
+UniRef100_UPI000401CEFF
+16161|scaffold59688_2|+220|00
+SRR5690606_35087643
+UniRef100_A0A0R3M5F3
+UniRef100_A0A969HBU9
+MGYP000847580960
+A0A0R3M5F3
+SRR5262249_39096779
+SRR5690348_3124064
+MGYP000666260026
+UniRef100_A0A838IZY5
+MGYP001366537082
+A0A1Q2HNV8
+UniRef100_UPI002011AF87
+23258|scaffold4609030_1|+1|11
+SRR5579871_6120579
+MGYP000105995723
+SRR3954468_6301146
+MGYP001433622665
+UniRef100_A0A3D6C093
+A0A1W6LJH8
+SRR5687767_11767969
+SRR4051794_40104329
+SRR3954454_22706284
+MGYP000010225417
+2271|Ga0209795_10171170_2|-245|01
+MGYP000738482073
+SRR6202012_1017248
+26133|Ga0268298_10010625_3|-7238|00
+SRR5262249_6174883
+UniRef100_UPI001904043C
+MGYP001146183833
+UniRef100_A0A2U2N9L6
+UniRef100_A0A127EN01
+A0A127EN01
+U2E7T8
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examples/data/provenance.md ADDED Viewed

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+# KaiB demo data — provenance
+This directory contains a concatenated demo asset for the SF-Cluster Colab
+notebook. It is derived from the SF-Cluster Phase II benchmark's KaiB
+`diverse_sf` arm and the FrustrAI-Seq per-residue Frustration Index (FI)
+outputs.
+## Files
+| File                  | Shape / size           | Description |
+|-----------------------|------------------------|-------------|
+| `KaiB_filtered.a3m`   | 364 records, L=91      | Subset of the KaiB filtered MSA. Query (`>101`, UniProt Q79V61 residues 5–95) is row 0. Lowercase insertion-state letters preserved. |
+| `KaiB_fi_matrix.npy`  | (364, 91) float32      | Per-residue FI matrix. Row `i` corresponds to record `i` in the A3M. |
+| `KaiB_seq_ids.txt`    | 364 lines              | One short sequence ID per line, in the same order as the A3M / FI matrix. |
+## Source paths (private dev repo, read-only)
+- Filtered MSA:
+  `/data1/hanqun/SF-Design/SF-Cluster/data/processed/msa/KaiB/KaiB/KaiB_KaiBTE_91aa_UniProt_Q79V61_5to95_2QKE_chainB.filtered.a3m`
+  (depth 6821, L=91)
+- FI artifacts (per-subset):
+  `/data1/hanqun/SF-Design/SF-Cluster/results/frustai_artifacts/KaiB/diverse_sf/KaiB/{000..011}/`
+  with files `fi_matrix.npy` ((32, 91) float32), `metadata.json`,
+  `fi_residual_matrix.npy`, `entropy_matrix.npy`.
+- Source subset A3Ms (used to map FI rows → sequence IDs):
+  `/data1/hanqun/SF-Design/SF-Cluster/results/baseline_p8/diverse_sf/KaiB/KaiB/screen/diversesf_KaiB_KaiB_seed{000..011}.a3m`.
+## Construction recipe
+1. For each of the 12 `diverse_sf` subsets, load `fi_matrix.npy` ((32, 91)
+   float32) and the corresponding `diversesf_KaiB_KaiB_seed{NNN}.a3m`.
+2. Concatenate rows in subset-index order; track the parallel sequence-ID
+   list from the A3M records.
+3. **Deduplication policy**: first occurrence wins. A sequence ID seen in an
+   earlier subset is skipped (both in the FI matrix and the ID list). This
+   reduces 12 × 32 = 384 raw rows to 364 unique rows.
+4. Extract the corresponding sequences (with their full headers and
+   lowercase insertion states) from the filtered MSA, preserving the order
+   established in step 3. The query (`>101`) is always row 0.
+All 364 unique IDs were found in the filtered MSA (0 missing).
+## Models
+- **FrustrAI-Seq weights**: HF repo `leuschj/FrustrAI-Seq`, commit
+  `ee5a01a29fde00630f4a1157f0e6cb8343ac434b`. Inference in fp16 with LoRA
+  adapters merged.
+## License
+This demo asset is released under MIT alongside the SF-Cluster OSS package.
+The KaiB sequence (UniProt Q79V61, *Thermosynechococcus elongatus*) and its
+MSA neighbors are public-domain sequence records via UniRef100 / Mgnify;
+no proprietary structures are included. FrustrAI-Seq outputs are derived
+features (floating-point FI values) and are released by the FrustrAI-Seq
+authors under their own license — see
+https://huggingface.co/leuschj/FrustrAI-Seq.