Update README.md

README.md

@@ -1,7 +1,11 @@
 ---
 license: apache-2.0
 tags:
-- generated_from_trainer
+- science
+- sourcedata
+- biology
+- biomedical
+- medical
 datasets:
 - source_data_nlp
 metrics:
@@ -28,6 +32,40 @@ model-index:
     - name: F1
       type: f1
       value: 0.9282082570673175
+widgets:
+- text: >-
+    Figure 2A. HEK293T cells were transfected with MYC-FOXP3 and FLAG-USP44
+    encoding expression constructs using Polyethylenimine. 48hrs
+    post-transfection, cells were harvested, lysed, and anti-FLAG or anti-MYC
+    antibody coated beads were used to immunoprecipitate the given labeled
+    protein along with its binding partner. Co-IP' ed proteins were subjected to
+    SDS PAGE followed by immunoblot analysis. Antibodies recognizing FLAG or MYC
+    tags were used to probe for USP44 and FOXP3, respectively. B. Endogenous
+    co-IP of USP44 and FOXP3 in murine iTregs. iTregs were generated as in Fig.
+    1 from naïve CD4+T cells FACS isolated from pooled suspensions of the lymph
+    node and spleen cells of wild type C57BL/6 mice (n = 2-3 / experiment).
+    iTregs were lysed and key proteins were immunoprecipitated using either
+    anti-USP44 (right panel) or anti-FOXP3 (left panel) antibody. Proteins
+    pulled-down in this experiment were then resolved and analyzed by immunoblot
+    using anti-FOXP3 or anti-USP44 antibodies. C. Endogenous co-IP of USP44 and
+    FOXP3 in murine nTregs. nTregs (CD4+CD25high) isolated by FACS were
+    activated by anti-CD3 and anti-CD28 (1 and 4 ug/ml, respectively) overnight
+    in the presence of IL-2 (100 U/ml). The cells were lysed and proteins were
+    immunoprecipitated using either anti-Foxp3 (left panel) or anti-Usp44 (right
+    panel). Proteins pulled down in this experiment were then resolved and
+    identified with the indicated antibodies. D . Naïve murine CD4+T cells were
+    isolated by FACS from lymph node and spleen cell suspension of USP44fl/fl
+    CD4Cre+ mice and that of their wild type littermates (USP44fl/fl
+    CD4Cre-mice; n = 2-3 / group / experiment) . iTreg cells were generated from
+    these mice as described for Fig. 1 before incubation on a microscope slide
+    pre-coated with poly-L lysine for 1h. Adhered cells were then fixed by PFA
+    for 0.5 followed by blocking with 1% BSA for 1h, then incubation with the
+    specified antibodies. Representative confocal microscopy images (40X) were
+    visualized for endogenous USP44 (red) and FOXP3 Baxter et al (). DAPI was
+    used to visualize cell nuclei (blue); scale bar 50μm.
+language:
+- en
+pipeline_tag: token-classification
 ---
 
 <!-- This model card has been generated automatically according to the information the Trainer had access to. You
@@ -80,4 +118,4 @@ The following hyperparameters were used during training:
 - Transformers 4.20.0
 - Pytorch 1.11.0a0+bfe5ad2
 - Datasets 1.17.0
-- Tokenizers 0.12.1
+- Tokenizers 0.12.1