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README.md
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# afMLevel-background-unet
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This U‑Net model predicts tilt, scanner drift, and other large‑scale imaging artifacts present in Atomic Force Microscopy (AFM) height maps.
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It outputs a **background** image, the same size and scale as the raw AFM image, which can be subtracted (via the accompanying [afMLevel](https://github.com/mayatek1/afMLevel)
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code) to produce a levelled height map.
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## Model Details
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### Model Description
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This model is
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- running inference,
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- subtracting the predicted background,
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- integrating the model into
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The model is a 7‑layer **U‑Net**, adapted from the original U‑Net architecture, and implemented fully in **PyTorch**.
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It performs image‑to‑image regression to estimate background height 'image' caused by physical and instrumental AFM artifacts.
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- **Developed by:** Maya Tekchandani
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- **Maintained by:** Dr Daniel E. Rollins
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- **Principal Investigator:** Dr George R. Heath
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- **Affiliation:** University of Leeds
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- **Funded by [optional]:** [More Information Needed]
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- **Shared by:** [Heath-
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- **Model type:** U‑Net regression model for AFM background
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- **License:** BSD‑3‑Clause
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- **Finetuned from model
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### Model Sources [optional]
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- **Repository:** https://github.com/mayatek1/afMLevel
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- **Paper
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- **Demo
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# Uses
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loaded using an external reader such as [playnano](https://github.com/derollins/playNano), [AFMReader](https://github.com/AFM-SPM/AFMReader), or a custom
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loader. Once loaded, the afMLevel repo and notebooks handle inference and output of either
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the predicted background or the levelled image directly.
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The model
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context, though it may generalise to other sample types with similar imaging characteristics.
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natural end-to-end workflow.
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- Batch levelling of **high-speed AFM videos** via playnano.
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- As a preprocessing step feeding into segmentation, particle detection, or other analysis
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tools.
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This model is **not** intended for:
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- denoising
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- non
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# Bias, Risks, and Limitations
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- The model was trained on a specific dataset of real AFM height maps; performance may degrade for very different imaging modes, scan sizes, or materials.
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- Extremely noisy scans or those containing jump‑to‑contact instabilities may produce inaccurate background predictions.
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- Users should visually inspect levelled outputs before scientific interpretation.
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### Recommendations
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- Avoid applying the model to
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## How to Get Started with the Model
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[afMLevel](https://github.com/mayatek1/afMLevel) repository, which handles inference,
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background subtraction, and output. Demonstration notebooks are available
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[here](https://github.com/mayatek1/afMLevel/tree/main/notebooks).
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## Training Details
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The model was trained from scratch on real AFM topography data using
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### Training Data
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This model was trained on a **non‑public dataset of 2,001 real AFM height‑map images**.
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To increase dataset size and improve generalization, images were augmented using:
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- reflection along the y
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- rotation by 180°
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- (mask model only) synthetic line-noise artefacts.
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This produced **6,003 training images**
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A **60:40 train‑validation split** was used.
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### Training Procedure
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- **Architecture:** 7‑layer U‑Net with large convolutional filters (9×9)
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- **Framework:** PyTorch
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- **Optimizer:** Adam
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- **Learning rate:** 0.0005
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- **Objective:** pixel‑wise continuous regression
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- **Hardware:** trained
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- Loss
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#### Preprocessing
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[More Information Needed]
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#### Training Hyperparameters
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- **Training regime:** [More Information Needed]
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#### Speeds, Sizes, Times [optional]
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[More Information Needed]
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## Evaluation
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### Testing Data
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#### Factors
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<!-- These are the evaluation metrics being used, ideally with a description of why. -->
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[More Information Needed]
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### Results
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#### Summary
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## Citation [optional]
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Paper in preparation
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**BibTeX:**
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[More Information Needed]
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**APA:**
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[More Information Needed]
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## Model Card Authors
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- **Maya Tekchandani** (primary developer)
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- **Dr Daniel E. Rollins** (maintainer)
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- **Dr George R. Heath** (project supervisor & PI)
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# Contact
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For questions or issues, please contact:
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**George R. Heath
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Email: G.R.Heath@leeds.ac.uk
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# afMLevel-background-unet
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This U‑Net model predicts tilt, scanner drift, and other large‑scale imaging artifacts present in Atomic Force Microscopy (AFM) height maps.
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+
It outputs a **background** image, the same size and scale as the raw AFM image, which can be subtracted (via the accompanying [afMLevel](https://github.com/mayatek1/afMLevel)
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code) to produce a levelled height map.
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## Model Details
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This model is part of the [afMLevel](https://github.com/mayatek1/afMLevel) project.
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### Model Description
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This model is a 7‑layer **U‑Net** architecture implemented in **PyTorch**, trained to perform image‑to‑image regression for background prediction in AFM height maps. The network was trained on **256 × 256‑pixel images** and therefore expects inputs of this size at inference time.
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The afMLevel repository includes tools for:
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- running inference,
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- subtracting the predicted background,
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- integrating the model into AFM anasis workflows.
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- **Developed by:** Maya Tekchandani
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- **Maintained by:** Dr Daniel E. Rollins
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- **Principal Investigator:** Dr George R. Heath
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- **Affiliation:** University of Leeds
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- **Funded by [optional]:** [More Information Needed]
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- **Shared by:** [Heath-AFMab](https://heath-afm-lab.github.io/)
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- **Model type:** U‑Net regression model for AFM background estimation
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- **License:** BSD‑3‑Clause
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- **Finetuned from model:** None (trained from scratch)
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### Model Sources
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- **Repository:** https://github.com/mayatek1/afMLevel
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- **Paper:** In preparation
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- **Demo notebooks:** https://github.com/mayatek1/afMLevel/tree/main/notebooks
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## Uses
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This model is designed for used within the [afMLevel](https://github.com/mayatek1/afMLevel/) `background_model` module.
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### Direct Use
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The [afMLevel](https://github.com/mayatek1/afMLevel/) model aplication package operates on **NumPy arrays**, so raw AFM files must first be loaded using an external reader such as [playnano](https://github.com/derollins/playNano), [AFMReader](https://github.com/AFM-SPM/AFMReader), or a custom loader. Once loaded, afMLevel handles inference and outputs either the predicted background or the final levelled image.
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The model has been primarily tested on **biological AFM data**. It may generalise to other sample types with similar imaging characteristics.
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### Downstream Use
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- Integration into **playNano**, enabling end‑to‑end reading and levelling.
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- Batch levelling of **high‑speed AFM videos** via playNano.
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- Preprocessing for segmentation, particle detection, or other AFM analysis tools.
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### Out‑of‑Scope Use
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This model is **not** intended for:
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- prediction of physical or mechanical properties,
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- denoising heavily corrupted AFM scans outside the training distrution,
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- interpretation of AFM contact mechanics,
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- specialised AFM modes (KPFM, MFM, FMM, etc.) without validation,
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- non‑biological samples without performance verification.
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## Bias, Risks, and Limitations
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- The model was trained on a specific dataset of real AFM height maps; performance may degrade for very different imaging modes, scan sizes, or materials.
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- Extremely noisy scans or those containing jump‑to‑contact instabilities may produce inaccurate background predictions.
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- Users should visually inspect levelled outputs before scientific interpretation.
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### Recommendations
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- Manually verify a subset of levelled images.
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- Avoid applying the model to imaging modes it was not trained on.
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## How to Get Started with the Model
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Use the model through the [afMLevel](https://github.com/mayatek1/afMLevel) repository, which handles background prediction, subtraction, and output generation. Demonstration notebooks are provided in the repository.
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## Training Details
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The model was trained from scratch on real AFM topography data using PyTorch.
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### Training Data
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This model was trained on a **non‑public dataset of 2,001 real AFM height‑map images**.
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To increase dataset size and improve generalization, images were augmented using:
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- reflection along the y‑axis,
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- rotation by 180°.
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This produced **6,003 training images**.
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A **60:40 train‑validation split** was used.
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### Training Procedure
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- **Architecture:** 7‑layer U‑Net with large convolutional filters (9×9)
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- **Framework:** PyTorch
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- **Optimizer:** Adam
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- **Learning rate:** 0.0005
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- **Objective:** pixel‑wise continuous regression
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- **Hardware:** trained with GPU acceleration
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- Loss curves were monitored to assess convergence.
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#### Preprocessing
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[More Information Needed]
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#### Training Hyperparameters
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- **Training regime:** [More Information Needed]
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#### Speeds, Sizes, Times
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[More Information Needed]
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## Evaluation
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The performance of the background model was evaluated indirectly through its impact on automated levelling. The main metric used was **Mean Squared Error (MSE)** between the auto‑levelled output and manually levelled ground‑truth images. Visual inspection was also carried out by the developers. Full evaluation results will be provided in the accompanying paper (in preparation).
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### Testing Data
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Evaluation was performed on a held‑out set of real AFM height maps spanning a wide range of:
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- biological sale types,
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- imaging conditions,
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- noise levels,
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- numbers of surface planes,
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- scan artefacts (e.g., streaks, line noise).
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*A dataset link will be added when appropriate.*
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### Metrics
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- **Primary metric:** MSE between auto‑levelled and manually levelled images
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- **Distribution analysis:** comparing mean vs median MSE
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- **Success‑rate metric:** proportion of images with MSE < 0.1 (empirical “well‑levelled” threshold)
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### Results
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Initial internal testing indicates that the background model supports reliable automated levelling across a broad range of AFM images. Full quantitative and statistical analyses will be included in the companion paper (in preparation).
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## Citation
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Paper in praration
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**BibTeX:**
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[More Inrmation Needed]
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**APA:**
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[More Information Needed]
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## Model Card Authors
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- **Maya Tekchandani**
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- **Dr Daniel E. Rollins**
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- **Dr George R. Heath**
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## Contact
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For questions or issues, please contact:
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**George R. Heath, University of Leeds**
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Email: G.R.Heath@leeds.ac.uk
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