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import torch
import torch.nn as nn
import torch.nn.functional as F
from torchvision import models, transforms
from PIL import Image
import numpy as np
import cv2
from flask import Flask, request, jsonify
from flask_cors import CORS
from flasgger import Swagger, swag_from
from ultralytics import YOLO
import io
import base64
import logging
# Load environment variables from .env file if it exists
try:
from dotenv import load_dotenv
load_dotenv()
except ImportError:
pass
# Production-ready logging configuration
DEBUG_MODE = os.getenv('DEBUG', 'False').lower() == 'true'
log_level = logging.DEBUG if DEBUG_MODE else logging.INFO
logging.basicConfig(level=log_level, format='%(asctime)s - %(levelname)s - %(message)s')
logger = logging.getLogger(__name__)
app = Flask(__name__)
CORS(app)
# Swagger configuration
swagger_config = {
"headers": [],
"specs": [
{
"endpoint": 'apispec',
"route": '/apispec.json',
"rule_filter": lambda rule: True,
"model_filter": lambda tag: True,
}
],
"static_url_path": "/flasgger_static",
"swagger_ui": True,
"specs_route": "/docs"
}
swagger_template = {
"swagger": "2.0",
"info": {
"title": "Lung Cancer Classification API with Grad-CAM",
"description": "API for classifying lung cancer types using DenseNet121 with Grad-CAM visualization",
"version": "1.0.0",
"contact": {
"name": "API Support"
}
},
"host": "localhost:5001",
"basePath": "/",
"schemes": ["http"],
"consumes": ["multipart/form-data", "application/json"],
"produces": ["application/json", "image/jpeg"]
}
swagger = Swagger(app, config=swagger_config, template=swagger_template)
# --- 1. CONFIGURATION ---
MODEL_PATH = 'models/densenet_final_classification.pth'
YOLO_MODEL_PATH = 'models/best.pt'
NUM_CLASSES = 4
PADDING_FACTOR = 0.20 # 20% context margin around detected tumors
LABELS = [
'Adenocarcinoma (Class A)',
'Small Cell (Class B)',
'Large Cell (Class E)',
'Squamous Cell (Class G)'
]
# --- 2. MODEL SETUP ---
class GradCAM:
def __init__(self, model, target_layer):
self.model = model
self.target_layer = target_layer
self.activations = None
self.target_layer.register_forward_hook(self._save_activations)
def _save_activations(self, module, input, output):
self.activations = output
def generate_heatmap(self, input_image, class_idx=None):
self.model.eval()
self.activations = None
output = self.model(input_image)
if class_idx is None:
class_idx = torch.argmax(output).item()
# Compute gradients directly w.r.t. activations β avoids backward hook + view issue
grads = torch.autograd.grad(
outputs=output[0, class_idx],
inputs=self.activations,
retain_graph=False,
create_graph=False
)[0]
# Pool gradients over spatial dimensions
pooled_gradients = torch.mean(grads, dim=[0, 2, 3])
# Weight activations by pooled gradients
activations = self.activations.detach()
weighted = torch.sum(
activations * pooled_gradients.view(1, -1, 1, 1),
dim=1
).squeeze()
# Apply ReLU and normalize
heatmap = F.relu(weighted)
max_val = torch.max(heatmap)
if max_val > 0:
heatmap = heatmap / max_val
return heatmap.cpu().numpy(), LABELS[class_idx], torch.softmax(output, dim=1)[0][class_idx].item()
def load_model():
# DenseNet121 is the common backbone for these tasks
logger.info("π§ Loading DenseNet121 Model...")
model = models.densenet121(weights=None)
num_ftrs = model.classifier.in_features # 1024
# Classifier matching densenet_final_classification.pth:
# State dict has parameters at index 1 (Linear layer)
# Index 0 is a non-parameter layer (likely ReLU as per README)
model.classifier = nn.Sequential(
nn.ReLU(), # classifier.0
nn.Linear(num_ftrs, NUM_CLASSES) # classifier.1 (has weight and bias)
)
if os.path.exists(MODEL_PATH):
model.load_state_dict(torch.load(MODEL_PATH, map_location=torch.device('cpu')))
logger.info(f"β
Model loaded from {MODEL_PATH}")
# === NEW: Verify weight distribution ===
classifier = model.classifier
# Check output layer (final Linear layer at index 1)
output_layer = classifier[1] # nn.Linear(num_ftrs, 4)
final_bias = output_layer.bias.data
final_weight = output_layer.weight.data
logger.warning("\n" + "="*60)
logger.warning("π MODEL WEIGHT ANALYSIS (At Startup)")
logger.warning("="*60)
logger.info(f"Output layer bias values: {[f'{x.item():.4f}' for x in final_bias]}")
logger.info("Output layer weight stats:")
for i, label in enumerate(LABELS):
weight_mean = final_weight[i].mean().item()
weight_std = final_weight[i].std().item()
bias_val = final_bias[i].item()
logger.info(f" {label}: weight_mean={weight_mean:.4f}, weight_std={weight_std:.4f}, bias={bias_val:.4f}")
# Check for extreme imbalance
class_b_bias = final_bias[1].item() # Class B is index 1
class_b_weight_mean = final_weight[1].mean().item()
if class_b_bias > 1.5 or class_b_weight_mean > 0.3:
logger.warning("β οΈ CLASS B (SMALL CELL) BIAS DETECTED!")
logger.warning(f" Bias value: {class_b_bias:.4f} (should be close to other classes)")
logger.warning(f" Weight mean: {class_b_weight_mean:.4f}")
logger.warning(" This explains why all images are classified as Class B.")
logger.warning(" Root cause: Model trained on imbalanced data or needs retraining.")
logger.warning("="*60 + "\n")
else:
logger.warning(f"β οΈ Model file not found at {MODEL_PATH}. Using uninitialized model.")
model.eval()
return model
model = load_model()
# Load YOLO model for tumor detection
logger.info("ποΈ Loading YOLO Detection Model...")
if os.path.exists(YOLO_MODEL_PATH):
yolo_model = YOLO(YOLO_MODEL_PATH)
logger.info(f"β
YOLO model loaded from {YOLO_MODEL_PATH}")
else:
logger.warning(f"β οΈ YOLO model file not found at {YOLO_MODEL_PATH}")
yolo_model = None
# DenseNet target layer is usually the last feature block
target_layer = model.features.norm5
cam = GradCAM(model, target_layer)
# Confidence threshold β below this we treat the result as uncertain
CONFIDENCE_THRESHOLD = 0.5
# --- 3. IMAGE PREPROCESSING ---
# Normalize with ImageNet stats (standard for DenseNet pretrained backbone)
preprocess = transforms.Compose([
transforms.Resize((224, 224)),
transforms.ToTensor(),
transforms.Normalize(mean=[0.485, 0.456, 0.406], std=[0.229, 0.224, 0.225]),
])
preprocess_no_norm = transforms.Compose([
transforms.Resize((224, 224)),
transforms.ToTensor(),
])
def is_ct_scan(image_pil):
"""
Validate that the image is likely a CT scan.
CT scans are grayscale β R, G, B channels are nearly identical.
Color photos (faces, etc.) have high inter-channel variance.
"""
img_np = np.array(image_pil.resize((64, 64))).astype(np.float32)
r, g, b = img_np[:,:,0], img_np[:,:,1], img_np[:,:,2]
# Mean absolute difference between channels
rg_diff = np.mean(np.abs(r - g))
rb_diff = np.mean(np.abs(r - b))
gb_diff = np.mean(np.abs(g - b))
color_score = (rg_diff + rb_diff + gb_diff) / 3.0
# CT scans are grayscale: channel diff < threshold
# Real CT scans: typically 2-5
# Color photos (faces): 7-100
# Threshold: 6.0
is_valid = color_score < 6.0
if is_valid:
logger.info(f"β
Valid CT scan detected (color_score={color_score:.2f})")
else:
logger.warning(f"β Not a CT scan (color_score={color_score:.2f} >= 6.0)")
return is_valid, round(float(color_score), 2)
def apply_heatmap(image_pil, heatmap):
# Resize heatmap to match image size
heatmap = cv2.resize(heatmap, (image_pil.size[0], image_pil.size[1]))
heatmap = np.uint8(255 * heatmap)
heatmap = cv2.applyColorMap(heatmap, cv2.COLORMAP_JET)
img_np = np.array(image_pil)
# Convert RGB (PyTorch/PIL) to BGR (OpenCV)
img_np = cv2.cvtColor(img_np, cv2.COLOR_RGB2BGR)
superimposed_img = heatmap * 0.4 + img_np
superimposed_img = np.clip(superimposed_img, 0, 255).astype(np.uint8)
# Convert BGR (OpenCV) back to RGB (PyTorch/PIL)
return cv2.cvtColor(superimposed_img, cv2.COLOR_BGR2RGB)
def apply_heatmap_to_full_image(image_pil, heatmap_crop, crop_coords):
"""
Apply heatmap from cropped tumor region to full image.
Args:
image_pil: Full original image (PIL)
heatmap_crop: Heatmap generated from crop (numpy array)
crop_coords: (crop_x1, crop_y1, crop_x2, crop_y2) coordinates
Returns:
Full image with heatmap overlay
"""
crop_x1, crop_y1, crop_x2, crop_y2 = crop_coords
img_h, img_w = image_pil.size[1], image_pil.size[0]
# Create full-size heatmap initialized to zeros
full_heatmap = np.zeros((img_h, img_w), dtype=np.float32)
# Resize cropped heatmap to match crop size
crop_h = crop_y2 - crop_y1
crop_w = crop_x2 - crop_x1
resized_heatmap = cv2.resize(heatmap_crop, (crop_w, crop_h))
# Place resized heatmap at correct location in full image
full_heatmap[crop_y1:crop_y2, crop_x1:crop_x2] = resized_heatmap
# Normalize to 0-1 range
max_val = np.max(full_heatmap)
if max_val > 0:
full_heatmap = full_heatmap / max_val
# Apply color mapping
full_heatmap = np.uint8(255 * full_heatmap)
colored_heatmap = cv2.applyColorMap(full_heatmap, cv2.COLORMAP_JET)
img_np = np.array(image_pil)
# Convert RGB (PyTorch/PIL) to BGR (OpenCV)
img_np = cv2.cvtColor(img_np, cv2.COLOR_RGB2BGR)
# Blend with original image (40% heatmap, 60% original)
superimposed_img = colored_heatmap.astype(np.float32) * 0.4 + img_np.astype(np.float32) * 0.6
superimposed_img = np.clip(superimposed_img, 0, 255).astype(np.uint8)
# Convert BGR (OpenCV) back to RGB (PyTorch/PIL)
return cv2.cvtColor(superimposed_img, cv2.COLOR_BGR2RGB)
# --- 4. ROUTES ---
@app.route('/', methods=['GET'])
def home():
"""
Home endpoint
---
tags:
- General
responses:
200:
description: API information and available endpoints
schema:
type: object
properties:
status:
type: string
example: running
message:
type: string
example: Lung Cancer Classification API with Grad-CAM
endpoints:
type: object
model:
type: string
example: models/densenet_final_classification.pth
classes:
type: array
items:
type: string
"""
return jsonify({
'status': 'running',
'message': 'Lung Cancer Classification API with Grad-CAM',
'endpoints': {
'/health': 'GET - Check API health and model status',
'/validate-ct': 'POST - Check if image is a CT scan (no classification)',
'/analyze': 'POST - Upload image for Grad-CAM analysis (multipart/form-data with "file" field)',
'/docs': 'GET - Swagger UI documentation'
},
'model': MODEL_PATH,
'classes': LABELS
})
@app.route('/validate-ct', methods=['POST'])
def validate_ct():
"""
Validate if uploaded image is a CT scan WITHOUT running classification
---
tags:
- Validation
parameters:
- name: file
in: formData
type: file
required: true
description: Medical image file (JPEG, PNG, etc.)
consumes:
- multipart/form-data
produces:
- application/json
responses:
200:
description: CT scan validation result
schema:
type: object
properties:
is_ct_scan:
type: boolean
example: true
color_score:
type: number
format: float
example: 5.2
message:
type: string
example: Valid CT scan - grayscale image detected
"""
logger.info("=== /validate-ct endpoint called ===")
if 'file' not in request.files:
return jsonify({'error': 'No file uploaded'}), 400
file = request.files['file']
if file.filename == '':
return jsonify({'error': 'No file selected'}), 400
try:
logger.info(f"Validating file: {file.filename}")
img_bytes = file.read()
image = Image.open(io.BytesIO(img_bytes)).convert('RGB')
logger.info(f"Image loaded, size: {image.size}, mode: {image.mode}")
valid_ct, color_score = is_ct_scan(image)
# Convert numpy types to Python native types for JSON serialization
valid_ct = bool(valid_ct)
color_score = float(color_score)
logger.info(f"Validation result: {valid_ct}, color_score: {color_score}")
return jsonify({
'is_ct_scan': valid_ct,
'color_score': color_score,
'message': f"{'Valid CT scan - grayscale image detected' if valid_ct else f'NOT a CT scan - color image detected (color_score={color_score})'}"
})
except Exception as e:
logger.error(f"Error validating image: {str(e)}", exc_info=True)
return jsonify({'error': str(e)}), 500
@app.route('/analyze', methods=['POST'])
def analyze():
"""
Analyze lung cancer image with YOLO detection + DenseNet classification + Grad-CAM
---
tags:
- Analysis
parameters:
- name: file
in: formData
type: file
required: true
description: Medical image file (JPEG, PNG, etc.)
consumes:
- multipart/form-data
produces:
- application/json
responses:
200:
description: Successfully analyzed image
schema:
type: object
properties:
success:
type: boolean
example: true
tumors_detected:
type: integer
example: 1
detections:
type: array
items:
type: object
properties:
tumor_id:
type: integer
bbox:
type: array
items: [x1, y1, x2, y2]
prediction:
type: string
confidence:
type: number
all_confidences:
type: object
crop_image:
type: string
heatmap_image:
type: string
original_image:
type: string
description: Base64 encoded original image with detection boxes
400:
description: Bad request or no tumors detected
"""
logger.warning("\n" + "="*80)
logger.warning("=== /analyze endpoint called (YOLO + DenseNet + Grad-CAM) ===")
logger.warning("="*80)
if 'file' not in request.files:
return jsonify({'error': 'No file uploaded'}), 400
file = request.files['file']
if file.filename == '':
return jsonify({'error': 'No file selected'}), 400
try:
logger.info(f"π Processing file: {file.filename}")
img_bytes = file.read()
# Load image as BGR (OpenCV format)
original_img = cv2.imdecode(np.frombuffer(img_bytes, np.uint8), cv2.IMREAD_COLOR)
if original_img is None:
return jsonify({'error': 'Could not load image'}), 400
h, w = original_img.shape[:2]
logger.info(f"β
Image loaded - Size: {w}x{h}")
# STEP 1: YOLO DETECTION
logger.info("\n>>> STEP 1: YOLO Tumor Detection")
if yolo_model is None:
return jsonify({'error': 'YOLO model not loaded'}), 500
results = yolo_model.predict(source=original_img, device='cpu', conf=0.15, verbose=False)
boxes = results[0].boxes
logger.info(f"π Detected {len(boxes)} tumor(s)")
if len(boxes) == 0:
logger.warning("βΉοΈ No tumors detected")
# Still return the original image
pil_img = Image.fromarray(cv2.cvtColor(original_img, cv2.COLOR_BGR2RGB))
original_io = io.BytesIO()
pil_img.save(original_io, 'JPEG', quality=85)
original_io.seek(0)
original_base64 = base64.b64encode(original_io.getvalue()).decode('utf-8')
return jsonify({
'success': True,
'tumors_detected': 0,
'detections': [],
'original_image': original_base64,
'message': 'No tumors detected in this image'
})
# STEP 2: CLASSIFY EACH DETECTED TUMOR
logger.info("\n>>> STEP 2: Classifying Detected Tumors")
detections = []
segmentation_img = original_img.copy()
# Track highest confidence tumor for heatmap display
highest_conf_idx = -1
highest_conf_value = 0
highest_conf_heatmap = None
highest_conf_crop_pil = None
highest_conf_crop_coords = None
for tumor_idx, box in enumerate(boxes):
logger.info(f"\n--- Processing Tumor {tumor_idx + 1} ---")
# Convert numpy types to Python ints for JSON serialization
x1, y1, x2, y2 = [int(v) for v in box.xyxy[0].numpy()]
# Add padding
box_w, box_h = x2 - x1, y2 - y1
pad_w = int(box_w * PADDING_FACTOR)
pad_h = int(box_h * PADDING_FACTOR)
crop_x1 = int(max(0, x1 - pad_w))
crop_y1 = int(max(0, y1 - pad_h))
crop_x2 = int(min(w, x2 + pad_w))
crop_y2 = int(min(h, y2 + pad_h))
logger.info(f" Box: [{x1}, {y1}, {x2}, {y2}]")
logger.info(f" Crop (with padding): [{crop_x1}, {crop_y1}, {crop_x2}, {crop_y2}]")
# Crop tumor region from color image
tumor_crop_bgr = original_img[crop_y1:crop_y2, crop_x1:crop_x2]
# Convert BGR (OpenCV) to RGB (PyTorch)
tumor_crop_rgb = cv2.cvtColor(tumor_crop_bgr, cv2.COLOR_BGR2RGB)
pil_crop = Image.fromarray(tumor_crop_rgb)
# Try both preprocessing approaches
logger.info(" Running inference with NORMALIZED preprocessing...")
input_norm = preprocess(pil_crop).unsqueeze(0)
logger.info(" Running inference with NON-NORMALIZED preprocessing...")
input_nonorm = preprocess_no_norm(pil_crop).unsqueeze(0)
with torch.no_grad():
logits_norm = model(input_norm)[0]
logits_nonorm = model(input_nonorm)[0]
out_norm = torch.softmax(logits_norm, dim=0)
out_nonorm = torch.softmax(logits_nonorm, dim=0)
max_conf_norm = torch.max(out_norm).item()
max_conf_nonorm = torch.max(out_nonorm).item()
logger.info(f" Normalized max confidence: {max_conf_norm*100:.2f}%")
logger.info(f" Non-normalized max confidence: {max_conf_nonorm*100:.2f}%")
# DEBUG: Log raw logits
logger.debug(f" Raw logits (normalized): {[f'{x.item():.2f}' for x in logits_norm]}")
logger.debug(f" Raw logits (non-normalized): {[f'{x.item():.2f}' for x in logits_nonorm]}")
# Use whichever gives higher confidence
if max_conf_norm >= max_conf_nonorm:
input_tensor = input_norm
probs = out_norm
logits_used = logits_norm
logger.info(" β
Using NORMALIZED preprocessing")
else:
input_tensor = input_nonorm
probs = out_nonorm
logits_used = logits_nonorm
logger.info(" β
Using NON-NORMALIZED preprocessing")
confidence = torch.max(probs).item()
class_idx = torch.argmax(probs).item()
diagnosis = LABELS[class_idx]
logger.warning(f" π― Diagnosis: {diagnosis}")
logger.warning(f" π Confidence: {confidence*100:.2f}%")
# All class confidences
all_confidences = {
LABELS[i]: round(probs[i].item() * 100, 2)
for i in range(NUM_CLASSES)
}
logger.info(" π Full class breakdown:")
for i, (label, conf) in enumerate(all_confidences.items()):
logit_val = logits_used[i].item()
logger.info(f" - {label}: {conf}% (logit: {logit_val:.3f})")
# β οΈ BIAS DETECTION: Warn if Class B (index 1) is always winning
if class_idx == 1: # Class B is index 1
logger.warning(f" β οΈ WARNING: Class B detected (index 1). Check for model bias.")
# Generate Grad-CAM heatmap for this tumor
logger.info(" Generating Grad-CAM heatmap...")
heatmap_np, _, _ = cam.generate_heatmap(input_tensor, class_idx=class_idx)
# Track highest confidence tumor for main heatmap display
if confidence > highest_conf_value:
highest_conf_value = confidence
highest_conf_idx = tumor_idx
highest_conf_heatmap = heatmap_np
highest_conf_crop_pil = pil_crop
highest_conf_crop_coords = (crop_x1, crop_y1, crop_x2, crop_y2)
# Apply heatmap to crop
result_img = apply_heatmap(pil_crop, heatmap_np)
result_pil = Image.fromarray(result_img)
# Encode crop and heatmap as base64
crop_io = io.BytesIO()
pil_crop.save(crop_io, 'JPEG', quality=85)
crop_io.seek(0)
crop_base64 = base64.b64encode(crop_io.getvalue()).decode('utf-8')
heatmap_io = io.BytesIO()
result_pil.save(heatmap_io, 'JPEG', quality=85)
heatmap_io.seek(0)
heatmap_base64 = base64.b64encode(heatmap_io.getvalue()).decode('utf-8')
# Draw box on segmentation image for visualization
cv2.rectangle(segmentation_img, (x1, y1), (x2, y2), (0, 255, 0), 2)
label = f"Tumor {tumor_idx + 1}: {diagnosis.split(' ')[0]} ({confidence*100:.1f}%)"
cv2.putText(segmentation_img, label, (x1, y1 - 10), cv2.FONT_HERSHEY_SIMPLEX, 0.6, (0, 255, 0), 2)
detections.append({
'tumor_id': tumor_idx + 1,
'bbox': [int(x1), int(y1), int(x2), int(y2)],
'bbox_with_padding': [crop_x1, crop_y1, crop_x2, crop_y2],
'prediction': diagnosis,
'confidence': round(confidence * 100, 2),
'all_confidences': all_confidences,
'crop_image': crop_base64,
'heatmap_image': heatmap_base64
})
# Encode original image with detection boxes
detection_pil = Image.fromarray(cv2.cvtColor(segmentation_img, cv2.COLOR_BGR2RGB))
detection_io = io.BytesIO()
detection_pil.save(detection_io, 'JPEG', quality=85)
detection_io.seek(0)
detection_base64 = base64.b64encode(detection_io.getvalue()).decode('utf-8')
# Encode heatmap of highest confidence tumor on FULL IMAGE
full_original_pil = Image.fromarray(cv2.cvtColor(original_img, cv2.COLOR_BGR2RGB))
heatmap_display_img = apply_heatmap_to_full_image(
full_original_pil,
highest_conf_heatmap,
highest_conf_crop_coords
)
heatmap_display_pil = Image.fromarray(heatmap_display_img)
heatmap_display_io = io.BytesIO()
heatmap_display_pil.save(heatmap_display_io, 'JPEG', quality=85)
heatmap_display_io.seek(0)
heatmap_display_base64 = base64.b64encode(heatmap_display_io.getvalue()).decode('utf-8')
logger.warning("\n" + "="*80)
logger.warning("β
ANALYSIS COMPLETE - RETURNING SUCCESS RESPONSE")
logger.warning("="*80)
# Classification summary
class_counts = {}
for detection in detections:
pred = detection['prediction'].split(' ')[0] # Get first word (class name)
class_counts[pred] = class_counts.get(pred, 0) + 1
logger.warning("π CLASSIFICATION SUMMARY:")
for class_name, count in sorted(class_counts.items()):
pct = (count / len(detections)) * 100 if detections else 0
logger.warning(f" {class_name}: {count} tumor(s) ({pct:.1f}%)")
if len(detections) > 0 and 'Small' in class_counts:
pct_class_b = (class_counts.get('Small', 0) / len(detections)) * 100
if pct_class_b >= 80:
logger.warning(f"β οΈ HIGH CLASS B BIAS DETECTED: {pct_class_b:.0f}% classified as Small Cell!")
logger.warning("="*80 + "\n")
return jsonify({
'success': True,
'tumors_detected': len(boxes),
'detections': detections,
'detection_image': detection_base64,
'heatmap_image': heatmap_display_base64
})
except Exception as e:
logger.error(f"\nβ ERROR processing image: {str(e)}", exc_info=True)
return jsonify({'error': str(e)}), 500
@app.route('/health', methods=['GET'])
def health():
"""
Health check endpoint
---
tags:
- General
responses:
200:
description: API health status
schema:
type: object
properties:
status:
type: string
example: healthy
model_loaded:
type: boolean
example: true
"""
return jsonify({'status': 'healthy', 'model_loaded': os.path.exists(MODEL_PATH)})
if __name__ == '__main__':
port = int(os.getenv('PORT', 5001))
app.run(host='0.0.0.0', port=port, debug=DEBUG_MODE)
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