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"""zero-shot inference over every held-out target x (pca, marker) checkpoint."""
from pathlib import Path
import warnings, json, sys, pickle, argparse, numpy as np, pandas as pd, anndata as ad, scanpy as sc, scipy.sparse as sp, torch, torch.nn.functional as F
warnings.filterwarnings("ignore"); sc.settings.verbosity = 0
import os as _os
from pathlib import Path as _Path
PANDA_ROOT = _Path(_os.environ.get("PANDA_ROOT", str(_Path(__file__).resolve().parents[2])))
sys.path.insert(0, str(PANDA_ROOT))
from panda import PANDAEncoder
from sklearn.metrics import accuracy_score, f1_score, classification_report

ROOT = Path(str(PANDA_ROOT))
DEVICE = torch.device("cuda" if torch.cuda.is_available() else "cpu")


def infer(a, system, variant):
    ck = torch.load(ROOT / f"checkpoints/{system}/{variant}/panda_final.pt",
                    map_location=DEVICE, weights_only=False)
    classes = ck["classes"]; marker_genes = ck.get("marker_genes", [])
    stats = np.load(ROOT / f"data/corpus/{system}/harmonized/corpus_stats.npz", allow_pickle=True)
    pca = pickle.load(open(ROOT / f"data/corpus/{system}/harmonized/pca_basis.pkl", "rb"))
    hvgs = [str(g) for g in stats["shared_hvgs"]]
    hvg2i = {g: i for i, g in enumerate(hvgs)}
    # human->mouse symbol case-fold (same heuristic as zero_shot.py): corpora + markers.yaml
    # use mouse Title-case symbols; human targets (e.g. veres) ship ALL-CAPS HGNC symbols.
    # Without this the HVG intersection collapses to ~0 and predictions are meaningless.
    vn = a.var_names.astype(str)
    n_upper = sum(1 for g in vn[:1000] if g.isupper() and len(g) > 1)
    if n_upper > 500:
        a = a.copy()
        a.var_names = [g.capitalize() for g in vn]
        a.var_names_make_unique()
        print(f"[infer] case-folded {n_upper}/1000 uppercase symbols human->mouse", flush=True)
    common = [g for g in a.var_names.astype(str) if g in hvg2i]
    if len(common) < 0.2 * len(hvgs):
        print(f"[infer] WARNING: only {len(common)}/{len(hvgs)} corpus HVGs present in target; "
              f"predictions will be unreliable", flush=True)
    a_c = a[:, common].copy()
    sc.pp.normalize_total(a_c, target_sum=1e4); sc.pp.log1p(a_c)
    X = a_c.X.toarray().astype(np.float32) if sp.issparse(a_c.X) else a_c.X.astype(np.float32)
    Xf = np.zeros((a.n_obs, len(hvgs)), dtype=np.float32)
    Xf[:, np.array([hvg2i[g] for g in common])] = X
    Xz = np.clip((Xf - stats["mean"].astype(np.float32)) / stats["std"].astype(np.float32), -10, 10)
    Xpca = pca.transform(Xz).astype(np.float32)
    Xmark = None
    if variant == "marker":
        mv = np.zeros((a.n_obs, len(marker_genes)), dtype=np.float32)
        for j, g in enumerate(marker_genes):
            if g in a.var_names:
                col = a[:, g].X
                if sp.issparse(col): col = col.toarray()
                mv[:, j] = col.flatten().astype(np.float32)
        # prefer the training-corpus marker stats stored in the checkpoint; refitting on the
        # target puts the marker channel on a target-dependent scale the model never saw
        if ck.get("marker_mu") is not None and ck.get("marker_sig") is not None:
            mmu = np.asarray(ck["marker_mu"], dtype=np.float32)
            msig = np.asarray(ck["marker_sig"], dtype=np.float32)
        else:
            print("[infer] WARNING: checkpoint lacks marker_mu/sig; z-scoring markers on the "
                  "target itself (legacy behaviour, target-dependent scale)", flush=True)
            mmu = mv.mean(axis=0, keepdims=True); msig = mv.std(axis=0, keepdims=True) + 1e-6
        Xmark = np.clip((mv - mmu) / msig, -5, 5).astype(np.float32)
    model = PANDAEncoder(variant=variant, n_pca=50,
                         n_markers=len(marker_genes) if variant == "marker" else 0,
                         n_classes=len(classes), n_sub=3,
                         n_datasets=len(ck["datasets"])).to(DEVICE).eval()
    model.load_state_dict(ck["model"])
    preds, probs, coss = [], [], []
    with torch.no_grad():
        for i in range(0, a.n_obs, 4096):
            xb = torch.from_numpy(Xpca[i:i+4096]).to(DEVICE)
            xmb = torch.from_numpy(Xmark[i:i+4096]).to(DEVICE) if Xmark is not None else None
            aux = torch.zeros(len(xb), 2, device=DEVICE)
            out = model(xb, aux, x_markers=xmb, lam_dann=0.0)
            mc = model.max_sub_cos(out["z"])
            preds.append(mc.argmax(dim=1).cpu().numpy())
            coss.append(mc.max(dim=1).values.cpu().numpy())
            probs.append(F.softmax(mc / 0.07, dim=1).cpu().numpy())
    return (np.array([classes[i] for i in np.concatenate(preds)]),
            np.concatenate(probs), np.concatenate(coss), classes)


TARGETS = {
    "pan_skin": [
        ("dingwall", ROOT / "data/raw/GSE220977_combined.h5ad", None),
        # WARNING: all 4,683 sulic cells (incl. this 4,183-cell "test" slice) are inside
        # data/corpus/pan_skin/harmonized/corpus.h5ad (verified by barcode overlap 2026-08-19).
        # Scoring the standard corpus checkpoint here is a TRAIN-SET evaluation, not held-out.
        # Use scripts/pan_skin/92_retrain_with_sulic_anchor.py (500-cell anchor, rest held out)
        # for an honest Sulic number.
        ("sulic", ROOT / "data/corpus/pan_skin/held_out_labeled/sulic_GSE212673_test.h5ad", "canonical_label"),
        ("belote", ROOT / "data/corpus/pan_skin/held_out_labeled/belote_GSE151091_test.h5ad", "canonical_label"),
    ],
    "hematopoiesis": [
        ("nestorowa", ROOT / "data/corpus/hematopoiesis/held_out_labeled/nestorowa_GSE81682_test.h5ad", "cell_type"),
        ("dahlin", None, None),   # loaded per-file via loader (61k cells across 8 samples)
    ],
    "pancreas": [
        ("baron", ROOT / "data/corpus/pancreas/held_out_labeled/baron_GSE84133_mouse_test.h5ad", "canonical_label"),
        ("veres", ROOT / "data/corpus/pancreas/held_out_labeled/veres_GSE114412_test.h5ad", "canonical_label"),
    ],
}


def load_dahlin():
    """dahlin 61k held-out unlabeled hsc target, 8 sample files."""
    D = ROOT / "data/corpus/hematopoiesis/held_out_unlabeled/dahlin_extract"
    GT = {"SIGAB1":"WT","SIGAC1":"WT","SIGAD1":"WT","SIGAF1":"WT","SIGAG1":"WT",
          "SIGAH1":"WT","SIGAG8":"Kit_W41","SIGAH8":"Kit_W41"}
    parts = []
    for f in sorted(D.glob("*.txt.gz")):
        sample = f.name.split("_")[1].split(".")[0]
        df = pd.read_csv(f, sep="\t", compression="gzip", index_col=0)
        X = sp.csr_matrix(df.values.T.astype(np.float32))
        obs = pd.DataFrame(index=[f"{sample}_{bc}" for bc in df.columns.astype(str)])
        obs["sample"] = sample; obs["genotype"] = GT.get(sample, "unknown")
        var = pd.DataFrame(index=df.index.astype(str))
        parts.append(ad.AnnData(X=X, obs=obs, var=var))
    a = ad.concat(parts, join="outer")
    import mygene
    mg = mygene.MyGeneInfo()
    res = mg.querymany(a.var_names.astype(str).tolist(), scopes="ensembl.gene",
                       fields="symbol", species="mouse", verbose=False)
    id2sym = {r["query"]: r["symbol"] for r in res if "symbol" in r}
    syms = pd.Series(a.var_names.astype(str)).map(id2sym).values
    keep = pd.notna(syms)
    a = a[:, keep].copy(); a.var_names = syms[keep]; a.var_names_make_unique()
    return a


def process(system, variant):
    print(f"\n===== {system} / {variant} =====", flush=True)
    for tgt_name, tgt_path, tgt_label in TARGETS[system]:
        print(f"\n[{tgt_name}] loading", flush=True)
        if tgt_name == "dahlin":
            a = load_dahlin()
        else:
            a = ad.read_h5ad(tgt_path)
        print(f"[{tgt_name}] {a.shape}", flush=True)
        pred, probs, max_cos, classes = infer(a, system, variant)
        out_dir = ROOT / f"discovery/{system}/{variant}"
        out_dir.mkdir(parents=True, exist_ok=True)
        # max_cos is the genuine prototype cosine; max_prob is softmax(max_cos/0.07).
        # (earlier revisions wrote the softmax value under the name max_cos)
        pd.DataFrame({
            "cell_id": a.obs_names,
            "pred_label": pred,
            "max_cos": max_cos,
            "max_prob": probs.max(axis=1),
        }).to_csv(out_dir / f"{tgt_name}_predictions.csv", index=False)
        summary = {
            "system": system, "variant": variant, "target": tgt_name,
            "n_cells": int(a.n_obs), "n_classes_model": len(classes),
            "predicted_class_dist": pd.Series(pred).value_counts().head(30).to_dict(),
            "max_cos_p50": float(np.median(max_cos)),
            "max_cos_p05": float(np.quantile(max_cos, 0.05)),
            "max_prob_p50": float(np.median(probs.max(axis=1))),
            "max_prob_p05": float(np.quantile(probs.max(axis=1), 0.05)),
        }
        if tgt_label and tgt_label in a.obs.columns:
            y_true = a.obs[tgt_label].astype(str).values
            mask = np.isin(y_true, classes)
            if mask.sum() > 0:
                acc = accuracy_score(y_true[mask], pred[mask])
                f1 = f1_score(y_true[mask], pred[mask], average="macro", zero_division=0)
                rep = classification_report(y_true[mask], pred[mask],
                                            zero_division=0, output_dict=True)
                summary["labeled_eval"] = {
                    "n_eval": int(mask.sum()), "acc": float(acc),
                    "n_excluded_off_vocab": int((~mask).sum()),
                    "excluded_label_dist": pd.Series(y_true[~mask]).value_counts().head(20).to_dict(),
                    "macro_f1": float(f1), "per_class_report": rep,
                }
                print(f"[{tgt_name}] acc={acc:.4f} F1={f1:.4f} on {mask.sum()} labeled cells", flush=True)
        (out_dir / f"{tgt_name}_summary.json").write_text(json.dumps(summary, indent=2, default=str))
        print(f"[{tgt_name}] wrote {out_dir}/{tgt_name}_predictions.csv + summary.json", flush=True)


def main():
    ap = argparse.ArgumentParser()
    ap.add_argument("--systems", nargs="*", default=["pan_skin", "hematopoiesis", "pancreas"])
    ap.add_argument("--variants", nargs="*", default=["pca", "marker"])
    args = ap.parse_args()
    for sys_ in args.systems:
        for var in args.variants:
            process(sys_, var)


if __name__ == "__main__":
    main()