PANDA / panda /markers.yaml
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# Curated marker-channel input genes per system for PANDA-Marker variant.
#
# These are the SIBLING-DISCRIMINATING genes that PCA(50) tends to discard.
# They are concatenated directly onto PCA(50) as log-normalised expression,
# bypassing the PCA bottleneck for the low-variance signal that separates
# closely-related subtypes.
#
# Set size targets: 20-40 markers per system. Curated for BOTH:
# (a) each class's positive identity marker (recovers within-class)
# (b) sibling-pair contrast markers (e.g. Dkk4 for eccrine vs hair placode)
#
# All symbols are mouse gene symbols (case-fold applied at inference for
# cross-species targets like Veres).
pan_skin:
# eccrine placode / nascent-gland / hair-placode axis
- Dkk4 # eccrine placode > hair placode (Dingwall Fig 2)
- Lgr6 # placode marker (Epi3)
- Trpv6 # nascent eccrine gland (Epi5/Epi8)
- Edar # both placodes; hair > eccrine
- Shh # hair placode discriminator
- Sox9 # hair placode / HF-primary-germ
- Lhx2 # hair placode
- Foxi3 # placode induction
- Wnt6 # placode Wnt program
- Wnt7b # placode Wnt program
- Lef1 # Wnt signalling
- Bmp7 # placode
# eccrine/appendage identity
- En1 # spatial repressor of eccrine outside placode
- Grhl3 # eccrine-duct master TF
- Cldn6 # eccrine tight junction
- Kremen2 # placode / eccrine
# basal / IFE / spinous / granular
- Krt5 # basal
- Krt14 # basal
- Krt10 # spinous differentiation
- Krt1 # spinous differentiation
- Trp63 # basal master
# melanocyte MITF regulon
- Dct # melanocyte
- Mlana # melanocyte
- Tyrp1 # melanocyte
- Pmel # melanocyte
- Sox10 # neural crest / melanocyte
# endothelial / fibroblast / immune
- Pecam1 # endothelial
- Cdh5 # endothelial
- Col1a1 # fibroblast reticular
- Dcn # fibroblast papillary
- Ptprc # immune (CD45)
# dermal niche (EDEN — Dingwall §5)
- S100a4 # EDEN dermal niche
- Pdgfra # dermal mesenchyme
hematopoiesis:
# LT-HSC / stem
- Hlf # LT-HSC canonical (Komorowska 2017)
- Meis1 # HSC/MPP
- Mecom # LT-HSC
- Procr # LT-HSC (EPCR)
- Fgd5 # LT-HSC reporter
- Mllt3 # LT-HSC self-renewal
# MPP substates
- Cd48 # MPP1 vs LT
- Flt3 # MPP4/LMPP
- Sell # ST-HSC / MPP1 (CD62L)
- Slamf1 # SLAM CD150
# erythroid
- Klf1 # erythroid master TF
- Gata1 # erythroid/mega
- Car1 # erythroblast
- Car2 # erythroblast
- Blvrb # committed erythroid
- Hba-a1 # hemoglobin
# megakaryocyte
- Itga2b # CD41 megakaryocyte
- Pf4 # megakaryocyte
- Gp1bb # megakaryocyte
# lymphoid
- Dntt # pre-B / lymphoid
- Vpreb1 # pre-B lymphoid
- Vpreb3 # pre-B lymphoid
- Il7r # lymphoid progenitor
# myeloid / mast / basophil
- Elane # GMP / granulocyte
- Mpo # myeloid
- Cpa3 # basophil-mast lineage
- Ms4a2 # mast Fc-epsilon-RI
- Csf1r # macrophage
# macrophage antimicrobial (myeloid combinatorial identity per §10.3)
- Wfdc17
- Mmp8
- Ctss
pancreas:
# endocrine progenitor (Ngn3 → Fev → hormone axis)
- Neurog3 # Ngn3 endocrine progenitor
- Fev # Fev+ intermediate EP
- Pax4 # alpha/beta bipotential
- Insm1 # endocrine progenitor
- Neurod1 # beta / late endocrine
- Cbfa2t3 # endocrine progenitor
- Btbd17 # endocrine progenitor
# alpha lineage
- Arx # alpha master
- Irx1 # alpha
- Irx2 # alpha (Veres §7 finding)
- Mafb # alpha
- Gcg # glucagon
# beta lineage
- Nkx6-1 # beta master
- Mnx1 # beta
- Ins1 # insulin
- Ins2 # insulin
- Mafa # mature beta
- Pdx1 # pancreatic progenitor + beta
# delta / gamma / epsilon
- Sst # delta
- Hhex # delta
- Ppy # gamma (PP)
- Aqp3 # gamma
- Ghrl # epsilon
# exocrine / stromal
- Prss1 # acinar
- Cel # acinar
- Ptf1a # acinar/pro-endocrine
- Krt19 # ductal
- Sox9 # ductal / progenitor
# embryonic hematopoiesis contamination flag (per §10.3 finding)
- Hbb-bs
- Hba-a1