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UCL-CSSB/PlasmidRL-ICML / plannotate /Base_t0.95 /feature_breakdown.json
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{
"model": "Base_t0.95",
"n_sequences_annotated": 3147,
"total_hits": 22764,
"by_type": {
"CDS": 7711,
"promoter": 4281,
"rep_origin": 2980,
"terminator": 2875,
"misc_feature": 1410,
"protein_bind": 1276,
"enhancer": 605,
"polyA_signal": 385,
"misc_RNA": 336,
"ncRNA": 245,
"LTR": 202,
"RBS": 169,
"intron": 137,
"mobile_element": 43,
"gene": 36,
"repeat_region": 34,
"oriT": 23,
"sig_peptide": 8,
"misc_recomb": 4,
"3'UTR": 2,
"5'UTR": 2
},
"by_type_and_description": [
{
"type": "rep_origin",
"description": "high-copy-number ColE1/pMB1/pBR322/pUC origin of replication",
"count": 1537
},
{
"type": "terminator",
"description": "transcription terminator T1 from the E. coli rrnB gene",
"count": 1020
},
{
"type": "promoter",
"description": "bla",
"count": 815
},
{
"type": "terminator",
"description": "phage T7 early transcription terminator",
"count": 803
},
{
"type": "CDS",
"description": "\u03b2-lactamase; bla; confers resistance to ampicillin, carbenicillin, and related antibiotics",
"count": 679
},
{
"type": "CDS",
"description": "aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin\u00ae) in eukaryotes",
"count": 645
},
{
"type": "enhancer",
"description": "human cytomegalovirus immediate early enhancer",
"count": 581
},
{
"type": "CDS",
"description": "lac repressor; lacI; The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-\u03b2-D-thiogalactopyranoside (IPTG).",
"count": 573
},
{
"type": "promoter",
"description": "bacterial promoter (Registry of Standard Biological Parts BBa J23119)",
"count": 573
},
{
"type": "rep_origin",
"description": "Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin.",
"count": 536
},
{
"type": "CDS",
"description": "hfriFP is a fluorescent protein published in 2013, derived from Herbaspirillum frisingense.",
"count": 515
},
{
"type": "protein_bind",
"description": "The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-\u03b2-D-thiogalactopyranoside (IPTG). lac repressor encoded by lacI",
"count": 472
},
{
"type": "protein_bind",
"description": "CAP binding activates transcription in the presence of cAMP. E. coli catabolite activator protein",
"count": 437
},
{
"type": "promoter",
"description": "promoter for bacteriophage T7 RNA polymerase",
"count": 432
},
{
"type": "misc_feature",
"description": "basis of mobility region from pBR322",
"count": 412
},
{
"type": "promoter",
"description": "bacterial promoter (Registry of Standard Biological Parts BBa J23119) modified to end with an SpeI site",
"count": 380
},
{
"type": "CDS",
"description": "monomeric derivative of DsRed (Campbell et al., 2002)",
"count": 376
},
{
"type": "CDS",
"description": "tetracycline efflux protein; tet; confers resistance to tetracycline",
"count": 347
},
{
"type": "rep_origin",
"description": "f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis",
"count": 327
},
{
"type": "CDS",
"description": "Rop protein, which maintains plasmids at low copy number; rop",
"count": 317
},
{
"type": "CDS",
"description": "LacZ\u03b1 fragment of \u03b2-galactosidase; lacZ fragment",
"count": 314
},
{
"type": "terminator",
"description": "transcription terminator from phage lambda",
"count": 314
},
{
"type": "promoter",
"description": "promoter for the E. coli lac operon",
"count": 299
},
{
"type": "CDS",
"description": "composite tag for in vivo biotinylation, TEV protease cleavage, and 6xHis tagging",
"count": 292
},
{
"type": "misc_RNA",
"description": "guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system",
"count": 286
},
{
"type": "ncRNA",
"description": "Accession: RF00001 - 5S ribosomal RNA",
"count": 232
},
{
"type": "CDS",
"description": "replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica",
"count": 220
},
{
"type": "rep_origin",
"description": "replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication",
"count": 219
},
{
"type": "terminator",
"description": "transcription terminator for bacteriophage T7 RNA polymerase",
"count": 197
},
{
"type": "polyA_signal",
"description": "SV40 polyadenylation signal",
"count": 182
},
{
"type": "promoter",
"description": "promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase",
"count": 169
},
{
"type": "RBS",
"description": "efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)",
"count": 169
},
{
"type": "CDS",
"description": "solubility-enhancement tag derived from T7 phage gene 10B",
"count": 164
},
{
"type": "misc_feature",
"description": "pBluescript multiple cloning site",
"count": 142
},
{
"type": "misc_feature",
"description": "pUC18/19 multiple cloning site",
"count": 138
},
{
"type": "CDS",
"description": "Protein predicted: Swiss-Prot protein existence level 4. From Escherichia coli.",
"count": 133
},
{
"type": "promoter",
"description": "promoter for bacteriophage T3 RNA polymerase",
"count": 127
},
{
"type": "misc_feature",
"description": "central polypurine tract and central termination sequence of HIV-1",
"count": 123
},
{
"type": "promoter",
"description": "SV40 enhancer and early promoter",
"count": 119
},
{
"type": "promoter",
"description": "lacI",
"count": 118
},
{
"type": "CDS",
"description": "CAT_ECOLX - Experimental evidence at protein level: Swiss-Prot protein existence level 1. This enzyme is an effector of chloramphenicol resistance in bacteria. From Escherichia coli. This protein is used as a marker in many commonly used cloning vectors, such as pACYC184.",
"count": 113
},
{
"type": "terminator",
"description": "Escherichia coli rrnB; transcription terminator T1 from the E. coli rrnB gene",
"count": 109
},
{
"type": "LTR",
"description": "truncated 5' long terminal repeat (LTR) from HIV-1",
"count": 106
},
{
"type": "CDS",
"description": "9xHis affinity tag",
"count": 94
},
{
"type": "misc_feature",
"description": "woodchuck hepatitis virus posttranscriptional regulatory element",
"count": 89
},
{
"type": "promoter",
"description": "promoter for bacteriophage SP6 RNA polymerase",
"count": 86
},
{
"type": "protein_bind",
"description": "mutant version of attB; recombination site for the Gateway\u00ae BP reaction BP Clonase\u2122",
"count": 85
},
{
"type": "promoter",
"description": "E. coli promoter for tetracycline efflux protein gene",
"count": 85
},
{
"type": "promoter",
"description": "human cytomegalovirus (CMV) immediate early promoter",
"count": 84
},
{
"type": "polyA_signal",
"description": "bovine growth hormone polyadenylation signal",
"count": 83
},
{
"type": "protein_bind",
"description": "mutant version of attL; recombination site for the Gateway\u00ae LR reaction LR Clonase\u2122",
"count": 81
},
{
"type": "CDS",
"description": "large T antigen from SV40 (simian virus 40)",
"count": 80
},
{
"type": "promoter",
"description": "RNA polymerase III promoter for human U6 snRNA",
"count": 79
},
{
"type": "rep_origin",
"description": "SV40 origin of replication",
"count": 78
},
{
"type": "rep_origin",
"description": "Plasmids containing the RSF 1030 origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins.",
"count": 78
},
{
"type": "terminator",
"description": "transcription terminator T2 from the E. coli rrnB gene",
"count": 75
},
{
"type": "CDS",
"description": "TN5P_ECOLX - Also known as tnp. Experimental evidence at protein level: Swiss-Prot protein existence level 1. Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA- bound transposase creates a synaptic DNA complex. After nicking of the first DNA strand, excision of the transposon proceeds through a series of intermediates. The transposase then mediates the insertion of the transposon at a new site by strand transfer. The activity of the wild- type transposase is very low, and is further inhibited by dimerization with the transposase inhibitor (inh). From Escherichia coli.",
"count": 73
},
{
"type": "CDS",
"description": "chloramphenicol acetyltransferase; cat; confers resistance to chloramphenicol",
"count": 67
},
{
"type": "misc_feature",
"description": "The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.",
"count": 66
},
{
"type": "misc_feature",
"description": "universal suffix for all parts",
"count": 65
}
]
}

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