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UCL-CSSB/PlasmidRL-ICML / plannotate /RL /feature_breakdown.json
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{
"n_sequences_annotated": 3892,
"total_hits": 74381,
"by_type": {
"CDS": 34613,
"promoter": 14421,
"rep_origin": 7807,
"protein_bind": 6007,
"terminator": 4020,
"misc_feature": 3117,
"RBS": 2900,
"polyA_signal": 798,
"enhancer": 518,
"gene": 89,
"LTR": 39,
"intron": 27,
"mobile_element": 13,
"ncRNA": 4,
"oriT": 3,
"sig_peptide": 3,
"repeat_region": 2
},
"by_type_and_description": [
{
"type": "CDS",
"description": "tetracycline efflux protein; tet; confers resistance to tetracycline",
"count": 6771
},
{
"type": "rep_origin",
"description": "high-copy-number ColE1/pMB1/pBR322/pUC origin of replication",
"count": 4353
},
{
"type": "promoter",
"description": "Drosophila melanogaster actin 5C promoter",
"count": 3825
},
{
"type": "promoter",
"description": "bla",
"count": 3815
},
{
"type": "CDS",
"description": "aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin\u00ae) in eukaryotes",
"count": 3327
},
{
"type": "CDS",
"description": "lac repressor; lacI; The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-\u03b2-D-thiogalactopyranoside (IPTG).",
"count": 3029
},
{
"type": "promoter",
"description": "promoter for bacteriophage T7 RNA polymerase",
"count": 2985
},
{
"type": "misc_feature",
"description": "basis of mobility region from pBR322",
"count": 2934
},
{
"type": "CDS",
"description": "Rop protein, which maintains plasmids at low copy number; rop",
"count": 2930
},
{
"type": "protein_bind",
"description": "CAP binding activates transcription in the presence of cAMP. E. coli catabolite activator protein",
"count": 2929
},
{
"type": "protein_bind",
"description": "The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-\u03b2-D-thiogalactopyranoside (IPTG). lac repressor encoded by lacI",
"count": 2913
},
{
"type": "CDS",
"description": "composite tag for in vivo biotinylation, TEV protease cleavage, and 6xHis tagging",
"count": 2904
},
{
"type": "CDS",
"description": "Protein predicted: Swiss-Prot protein existence level 4. From Escherichia coli.",
"count": 2902
},
{
"type": "RBS",
"description": "efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)",
"count": 2900
},
{
"type": "promoter",
"description": "lacI",
"count": 2898
},
{
"type": "CDS",
"description": "thrombin recognition and cleavage site",
"count": 2882
},
{
"type": "rep_origin",
"description": "f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis",
"count": 2859
},
{
"type": "terminator",
"description": "transcription terminator for bacteriophage T7 RNA polymerase",
"count": 2853
},
{
"type": "CDS",
"description": "solubility-enhancement tag derived from T7 phage gene 10B",
"count": 2848
},
{
"type": "CDS",
"description": "9xHis affinity tag",
"count": 2747
},
{
"type": "CDS",
"description": "\u03b2-lactamase; bla; confers resistance to ampicillin, carbenicillin, and related antibiotics",
"count": 991
},
{
"type": "polyA_signal",
"description": "SV40 polyadenylation signal",
"count": 763
},
{
"type": "CDS",
"description": "large T antigen from SV40 (simian virus 40)",
"count": 759
},
{
"type": "CDS",
"description": "epitope tag from simian virus 5",
"count": 750
},
{
"type": "CDS",
"description": "puromycin N-acetyltransferase; pac from Streptomyces; confers resistance to puromycin",
"count": 635
},
{
"type": "terminator",
"description": "transcription terminator T1 from the E. coli rrnB gene",
"count": 539
},
{
"type": "terminator",
"description": "phage T7 early transcription terminator",
"count": 537
},
{
"type": "promoter",
"description": "bacterial promoter (Registry of Standard Biological Parts BBa J23119)",
"count": 525
},
{
"type": "rep_origin",
"description": "Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin.",
"count": 515
},
{
"type": "enhancer",
"description": "human cytomegalovirus immediate early enhancer",
"count": 507
},
{
"type": "CDS",
"description": "hfriFP is a fluorescent protein published in 2013, derived from Herbaspirillum frisingense.",
"count": 500
},
{
"type": "promoter",
"description": "E. coli promoter for tetracycline efflux protein gene",
"count": 91
},
{
"type": "protein_bind",
"description": "mutant version of attB; recombination site for the Gateway\u00ae BP reaction BP Clonase\u2122",
"count": 90
},
{
"type": "gene",
"description": "yeast selectable marker conferring kanamycin resistance (Wach et al., 1994)",
"count": 69
},
{
"type": "misc_feature",
"description": "universal suffix for all parts",
"count": 62
},
{
"type": "protein_bind",
"description": "mutant version of attB; core recombination site for the Gateway\u00ae BP reaction BP Clonase\u2122",
"count": 52
},
{
"type": "CDS",
"description": "LacZ\u03b1 fragment of \u03b2-galactosidase; lacZ fragment",
"count": 41
},
{
"type": "promoter",
"description": "Ashbya gossypii TEF promoter",
"count": 34
},
{
"type": "CDS",
"description": "stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)",
"count": 33
},
{
"type": "CDS",
"description": "\u03b2-lactamase lacking the signal sequence; bla(M); allows cytosolic expression of \u03b2-lactamase",
"count": 29
},
{
"type": "terminator",
"description": "transcription terminator from phage lambda",
"count": 25
},
{
"type": "misc_feature",
"description": "pUC18/19 multiple cloning site",
"count": 25
},
{
"type": "CDS",
"description": "replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)",
"count": 24
},
{
"type": "misc_feature",
"description": "S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence",
"count": 23
},
{
"type": "promoter",
"description": "promoter for the E. coli lac operon",
"count": 23
},
{
"type": "CDS",
"description": "gentamycin acetyltransferase; aacC1; confers resistance to gentamycin",
"count": 22
},
{
"type": "CDS",
"description": "aminoglycoside adenylyltransferase (Murphy, 1985); aadA; confers resistance to spectinomycin and streptomycin",
"count": 22
},
{
"type": "CDS",
"description": "orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis; S. cerevisiae URA3; yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)",
"count": 20
},
{
"type": "gene",
"description": "yeast selectable marker conferring hygromycin resistance",
"count": 20
},
{
"type": "promoter",
"description": "promoter for bacteriophage SP6 RNA polymerase",
"count": 18
},
{
"type": "CDS",
"description": "aminoglycoside phosphotransferase from E. coli confers resistance to hygromycin",
"count": 17
},
{
"type": "CDS",
"description": "three tandem HA epitope tags",
"count": 16
},
{
"type": "CDS",
"description": "REGN_LAMBD - Experimental evidence at protein level: Swiss-Prot protein existence level 1. Plays a role as a transcriptional antitermination factor that allows the virus to initiate its lytic phase. Activates expression of the delayed viral early genes by modifying host RNA polymerase (RNAP) into a form that is resistant to termination signals. This antitermination function requires the host NusA protein which serves to stabilize the interaction between antitermination protein N and host RNA polymerase. From Escherichia phage lambda (Bacteriophage lambda).",
"count": 16
},
{
"type": "rep_origin",
"description": "incP origin of replication",
"count": 16
},
{
"type": "promoter",
"description": "promoter for bacteriophage T3 RNA polymerase",
"count": 16
},
{
"type": "terminator",
"description": "putative bacterial transcription terminator",
"count": 16
},
{
"type": "rep_origin",
"description": "yeast 2\u03bc plasmid origin of replication",
"count": 15
},
{
"type": "promoter",
"description": "Drosophila melanogaster actin 5c promoter",
"count": 15
},
{
"type": "LTR",
"description": "truncated 5' long terminal repeat (LTR) from HIV-1",
"count": 15
},
{
"type": "promoter",
"description": "strong E. coli promoter; hybrid between the trp and lac UV5 promoters",
"count": 15
}
]
}

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