Buckets:
| { | |
| "n_sequences_annotated": 3892, | |
| "total_hits": 74381, | |
| "by_type": { | |
| "CDS": 34613, | |
| "promoter": 14421, | |
| "rep_origin": 7807, | |
| "protein_bind": 6007, | |
| "terminator": 4020, | |
| "misc_feature": 3117, | |
| "RBS": 2900, | |
| "polyA_signal": 798, | |
| "enhancer": 518, | |
| "gene": 89, | |
| "LTR": 39, | |
| "intron": 27, | |
| "mobile_element": 13, | |
| "ncRNA": 4, | |
| "oriT": 3, | |
| "sig_peptide": 3, | |
| "repeat_region": 2 | |
| }, | |
| "by_type_and_description": [ | |
| { | |
| "type": "CDS", | |
| "description": "tetracycline efflux protein; tet; confers resistance to tetracycline", | |
| "count": 6771 | |
| }, | |
| { | |
| "type": "rep_origin", | |
| "description": "high-copy-number ColE1/pMB1/pBR322/pUC origin of replication", | |
| "count": 4353 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "Drosophila melanogaster actin 5C promoter", | |
| "count": 3825 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "bla", | |
| "count": 3815 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin\u00ae) in eukaryotes", | |
| "count": 3327 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "lac repressor; lacI; The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-\u03b2-D-thiogalactopyranoside (IPTG).", | |
| "count": 3029 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "promoter for bacteriophage T7 RNA polymerase", | |
| "count": 2985 | |
| }, | |
| { | |
| "type": "misc_feature", | |
| "description": "basis of mobility region from pBR322", | |
| "count": 2934 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "Rop protein, which maintains plasmids at low copy number; rop", | |
| "count": 2930 | |
| }, | |
| { | |
| "type": "protein_bind", | |
| "description": "CAP binding activates transcription in the presence of cAMP. E. coli catabolite activator protein", | |
| "count": 2929 | |
| }, | |
| { | |
| "type": "protein_bind", | |
| "description": "The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-\u03b2-D-thiogalactopyranoside (IPTG). lac repressor encoded by lacI", | |
| "count": 2913 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "composite tag for in vivo biotinylation, TEV protease cleavage, and 6xHis tagging", | |
| "count": 2904 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "Protein predicted: Swiss-Prot protein existence level 4. From Escherichia coli.", | |
| "count": 2902 | |
| }, | |
| { | |
| "type": "RBS", | |
| "description": "efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)", | |
| "count": 2900 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "lacI", | |
| "count": 2898 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "thrombin recognition and cleavage site", | |
| "count": 2882 | |
| }, | |
| { | |
| "type": "rep_origin", | |
| "description": "f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis", | |
| "count": 2859 | |
| }, | |
| { | |
| "type": "terminator", | |
| "description": "transcription terminator for bacteriophage T7 RNA polymerase", | |
| "count": 2853 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "solubility-enhancement tag derived from T7 phage gene 10B", | |
| "count": 2848 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "9xHis affinity tag", | |
| "count": 2747 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "\u03b2-lactamase; bla; confers resistance to ampicillin, carbenicillin, and related antibiotics", | |
| "count": 991 | |
| }, | |
| { | |
| "type": "polyA_signal", | |
| "description": "SV40 polyadenylation signal", | |
| "count": 763 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "large T antigen from SV40 (simian virus 40)", | |
| "count": 759 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "epitope tag from simian virus 5", | |
| "count": 750 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "puromycin N-acetyltransferase; pac from Streptomyces; confers resistance to puromycin", | |
| "count": 635 | |
| }, | |
| { | |
| "type": "terminator", | |
| "description": "transcription terminator T1 from the E. coli rrnB gene", | |
| "count": 539 | |
| }, | |
| { | |
| "type": "terminator", | |
| "description": "phage T7 early transcription terminator", | |
| "count": 537 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "bacterial promoter (Registry of Standard Biological Parts BBa J23119)", | |
| "count": 525 | |
| }, | |
| { | |
| "type": "rep_origin", | |
| "description": "Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin.", | |
| "count": 515 | |
| }, | |
| { | |
| "type": "enhancer", | |
| "description": "human cytomegalovirus immediate early enhancer", | |
| "count": 507 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "hfriFP is a fluorescent protein published in 2013, derived from Herbaspirillum frisingense.", | |
| "count": 500 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "E. coli promoter for tetracycline efflux protein gene", | |
| "count": 91 | |
| }, | |
| { | |
| "type": "protein_bind", | |
| "description": "mutant version of attB; recombination site for the Gateway\u00ae BP reaction BP Clonase\u2122", | |
| "count": 90 | |
| }, | |
| { | |
| "type": "gene", | |
| "description": "yeast selectable marker conferring kanamycin resistance (Wach et al., 1994)", | |
| "count": 69 | |
| }, | |
| { | |
| "type": "misc_feature", | |
| "description": "universal suffix for all parts", | |
| "count": 62 | |
| }, | |
| { | |
| "type": "protein_bind", | |
| "description": "mutant version of attB; core recombination site for the Gateway\u00ae BP reaction BP Clonase\u2122", | |
| "count": 52 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "LacZ\u03b1 fragment of \u03b2-galactosidase; lacZ fragment", | |
| "count": 41 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "Ashbya gossypii TEF promoter", | |
| "count": 34 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)", | |
| "count": 33 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "\u03b2-lactamase lacking the signal sequence; bla(M); allows cytosolic expression of \u03b2-lactamase", | |
| "count": 29 | |
| }, | |
| { | |
| "type": "terminator", | |
| "description": "transcription terminator from phage lambda", | |
| "count": 25 | |
| }, | |
| { | |
| "type": "misc_feature", | |
| "description": "pUC18/19 multiple cloning site", | |
| "count": 25 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)", | |
| "count": 24 | |
| }, | |
| { | |
| "type": "misc_feature", | |
| "description": "S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence", | |
| "count": 23 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "promoter for the E. coli lac operon", | |
| "count": 23 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "gentamycin acetyltransferase; aacC1; confers resistance to gentamycin", | |
| "count": 22 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "aminoglycoside adenylyltransferase (Murphy, 1985); aadA; confers resistance to spectinomycin and streptomycin", | |
| "count": 22 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis; S. cerevisiae URA3; yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)", | |
| "count": 20 | |
| }, | |
| { | |
| "type": "gene", | |
| "description": "yeast selectable marker conferring hygromycin resistance", | |
| "count": 20 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "promoter for bacteriophage SP6 RNA polymerase", | |
| "count": 18 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "aminoglycoside phosphotransferase from E. coli confers resistance to hygromycin", | |
| "count": 17 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "three tandem HA epitope tags", | |
| "count": 16 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "REGN_LAMBD - Experimental evidence at protein level: Swiss-Prot protein existence level 1. Plays a role as a transcriptional antitermination factor that allows the virus to initiate its lytic phase. Activates expression of the delayed viral early genes by modifying host RNA polymerase (RNAP) into a form that is resistant to termination signals. This antitermination function requires the host NusA protein which serves to stabilize the interaction between antitermination protein N and host RNA polymerase. From Escherichia phage lambda (Bacteriophage lambda).", | |
| "count": 16 | |
| }, | |
| { | |
| "type": "rep_origin", | |
| "description": "incP origin of replication", | |
| "count": 16 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "promoter for bacteriophage T3 RNA polymerase", | |
| "count": 16 | |
| }, | |
| { | |
| "type": "terminator", | |
| "description": "putative bacterial transcription terminator", | |
| "count": 16 | |
| }, | |
| { | |
| "type": "rep_origin", | |
| "description": "yeast 2\u03bc plasmid origin of replication", | |
| "count": 15 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "Drosophila melanogaster actin 5c promoter", | |
| "count": 15 | |
| }, | |
| { | |
| "type": "LTR", | |
| "description": "truncated 5' long terminal repeat (LTR) from HIV-1", | |
| "count": 15 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "strong E. coli promoter; hybrid between the trp and lac UV5 promoters", | |
| "count": 15 | |
| } | |
| ] | |
| } |
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- 9.86 kB
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- 5c20b7544d8120eb738023f47efafa9b0fd19982d7807d2123c9932c73a7ffd2
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