Buckets:
| { | |
| "model": "SFT_t0.95", | |
| "n_sequences_annotated": 3999, | |
| "total_hits": 68080, | |
| "by_type": { | |
| "CDS": 27172, | |
| "promoter": 12317, | |
| "rep_origin": 8319, | |
| "terminator": 6286, | |
| "protein_bind": 5563, | |
| "misc_feature": 4570, | |
| "RBS": 1483, | |
| "enhancer": 619, | |
| "ncRNA": 465, | |
| "polyA_signal": 339, | |
| "misc_RNA": 330, | |
| "mobile_element": 195, | |
| "intron": 132, | |
| "LTR": 93, | |
| "oriT": 74, | |
| "gene": 33, | |
| "sig_peptide": 27, | |
| "repeat_region": 22, | |
| "5'UTR": 13, | |
| "3'UTR": 13, | |
| "misc_recomb": 9, | |
| "tRNA": 4, | |
| "misc_signal": 2 | |
| }, | |
| "by_type_and_description": [ | |
| { | |
| "type": "rep_origin", | |
| "description": "high-copy-number ColE1/pMB1/pBR322/pUC origin of replication", | |
| "count": 4111 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "bla", | |
| "count": 3044 | |
| }, | |
| { | |
| "type": "misc_feature", | |
| "description": "basis of mobility region from pBR322", | |
| "count": 2740 | |
| }, | |
| { | |
| "type": "protein_bind", | |
| "description": "The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-\u03b2-D-thiogalactopyranoside (IPTG). lac repressor encoded by lacI", | |
| "count": 2701 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "tetracycline efflux protein; tet; confers resistance to tetracycline", | |
| "count": 2423 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "lac repressor; lacI; The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-\u03b2-D-thiogalactopyranoside (IPTG).", | |
| "count": 2419 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "\u03b2-lactamase; bla; confers resistance to ampicillin, carbenicillin, and related antibiotics", | |
| "count": 2283 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "Rop protein, which maintains plasmids at low copy number; rop", | |
| "count": 2146 | |
| }, | |
| { | |
| "type": "protein_bind", | |
| "description": "CAP binding activates transcription in the presence of cAMP. E. coli catabolite activator protein", | |
| "count": 1917 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "promoter for bacteriophage T7 RNA polymerase", | |
| "count": 1890 | |
| }, | |
| { | |
| "type": "RBS", | |
| "description": "efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)", | |
| "count": 1483 | |
| }, | |
| { | |
| "type": "terminator", | |
| "description": "transcription terminator T1 from the E. coli rrnB gene", | |
| "count": 1285 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "aminoglycoside phosphotransferase; aph(3')-Ia; confers resistance to kanamycin in bacteria or G418 (Geneticin\u00ae) in eukaryotes", | |
| "count": 1269 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "LacZ\u03b1 fragment of \u03b2-galactosidase; lacZ fragment", | |
| "count": 1266 | |
| }, | |
| { | |
| "type": "rep_origin", | |
| "description": "Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells that contain a second plasmid with the ColE1 origin.", | |
| "count": 1244 | |
| }, | |
| { | |
| "type": "rep_origin", | |
| "description": "f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis", | |
| "count": 1214 | |
| }, | |
| { | |
| "type": "terminator", | |
| "description": "transcription terminator for bacteriophage T7 RNA polymerase", | |
| "count": 1146 | |
| }, | |
| { | |
| "type": "terminator", | |
| "description": "phage T7 early transcription terminator", | |
| "count": 1106 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "Protein predicted: Swiss-Prot protein existence level 4. From Escherichia coli.", | |
| "count": 1036 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "solubility-enhancement tag derived from T7 phage gene 10B", | |
| "count": 1025 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "lacI", | |
| "count": 956 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "promoter for the E. coli lac operon", | |
| "count": 891 | |
| }, | |
| { | |
| "type": "terminator", | |
| "description": "Escherichia coli rrnB; transcription terminator T1 from the E. coli rrnB gene", | |
| "count": 816 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "composite tag for in vivo biotinylation, TEV protease cleavage, and 6xHis tagging", | |
| "count": 719 | |
| }, | |
| { | |
| "type": "rep_origin", | |
| "description": "Plasmids containing the RSF 1030 origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins.", | |
| "count": 699 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "strong E. coli promoter; hybrid between the trp and lac UV5 promoters", | |
| "count": 683 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "E. coli promoter for tetracycline efflux protein gene", | |
| "count": 656 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "hfriFP is a fluorescent protein published in 2013, derived from Herbaspirillum frisingense.", | |
| "count": 622 | |
| }, | |
| { | |
| "type": "enhancer", | |
| "description": "human cytomegalovirus immediate early enhancer", | |
| "count": 610 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase", | |
| "count": 602 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "bacterial promoter (Registry of Standard Biological Parts BBa J23119)", | |
| "count": 591 | |
| }, | |
| { | |
| "type": "terminator", | |
| "description": "transcription terminator from phage lambda", | |
| "count": 583 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "9xHis affinity tag", | |
| "count": 583 | |
| }, | |
| { | |
| "type": "misc_feature", | |
| "description": "pUC18/19 multiple cloning site", | |
| "count": 562 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "Experimental evidence at protein level: Swiss-Prot protein existence level 1. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut. From Schistosoma japonicum (Blood fluke).", | |
| "count": 455 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "lacI (mutant); In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold.", | |
| "count": 450 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "6xHis affinity tag", | |
| "count": 444 | |
| }, | |
| { | |
| "type": "misc_feature", | |
| "description": "pBluescript multiple cloning site", | |
| "count": 431 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "araBAD; promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)", | |
| "count": 379 | |
| }, | |
| { | |
| "type": "ncRNA", | |
| "description": "Accession: RF00001 - 5S ribosomal RNA", | |
| "count": 371 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "TN5P_ECOLX - Also known as tnp. Experimental evidence at protein level: Swiss-Prot protein existence level 1. Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA- bound transposase creates a synaptic DNA complex. After nicking of the first DNA strand, excision of the transposon proceeds through a series of intermediates. The transposase then mediates the insertion of the transposon at a new site by strand transfer. The activity of the wild- type transposase is very low, and is further inhibited by dimerization with the transposase inhibitor (inh). From Escherichia coli.", | |
| "count": 367 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "CAT_ECOLX - Experimental evidence at protein level: Swiss-Prot protein existence level 1. This enzyme is an effector of chloramphenicol resistance in bacteria. From Escherichia coli. This protein is used as a marker in many commonly used cloning vectors, such as pACYC184.", | |
| "count": 367 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "RNAAM_ECOLI - Also known as trpH From Escherichia coli (strain K12).", | |
| "count": 357 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "tobacco etch virus (TEV) protease recognition and cleavage site", | |
| "count": 330 | |
| }, | |
| { | |
| "type": "terminator", | |
| "description": "transcription terminator T2 from the E. coli rrnB gene", | |
| "count": 326 | |
| }, | |
| { | |
| "type": "terminator", | |
| "description": "putative bacterial transcription terminator", | |
| "count": 319 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "KKA2_KLEPN - Also known as kan, nptII. Experimental evidence at protein level: Swiss-Prot protein existence level 1. Resistance to kanamycin, neomycin, paromomycin, ribostamycin, butirosin and gentamicin B. From Klebsiella pneumoniae.", | |
| "count": 310 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "L-arabinose regulatory protein; araC", | |
| "count": 299 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "monomeric derivative of DsRed (Campbell et al., 2002)", | |
| "count": 292 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "bacterial promoter (Registry of Standard Biological Parts BBa J23119) modified to end with an SpeI site", | |
| "count": 276 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "maltose binding protein from E. coli; malE (mutated); This version of the gene does not encode a signal sequence, so MBP will remain in the cytosol.", | |
| "count": 271 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "pIII; III; minor coat protein", | |
| "count": 268 | |
| }, | |
| { | |
| "type": "rep_origin", | |
| "description": "M13 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis", | |
| "count": 263 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "chloramphenicol acetyltransferase; cat; confers resistance to chloramphenicol", | |
| "count": 253 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "leader peptide from bacteriophage T7 gene 10 promotes efficient translation in E. coli", | |
| "count": 252 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "replication protein for the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica", | |
| "count": 233 | |
| }, | |
| { | |
| "type": "misc_RNA", | |
| "description": "guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system", | |
| "count": 231 | |
| }, | |
| { | |
| "type": "promoter", | |
| "description": "promoter for bacteriophage T3 RNA polymerase", | |
| "count": 225 | |
| }, | |
| { | |
| "type": "rep_origin", | |
| "description": "replication origin of the broad-host-range plasmid pBBR1 from Bordetella bronchiseptica; requires the pBBR1 Rep protein for replication", | |
| "count": 218 | |
| }, | |
| { | |
| "type": "CDS", | |
| "description": "\u03b2-lactamase lacking the signal sequence; bla(M); allows cytosolic expression of \u03b2-lactamase", | |
| "count": 186 | |
| } | |
| ] | |
| } |
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- 11.2 kB
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- c6eb7f57960d4c9e648449590f06d2a51f2b0d1e55c6c03a35d4cce7e0160a37
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