Answer: C


Question: I want to clone the rpmC gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: A


Answer: D


Answer: B


Answer: C


Answer: B


Answer: D


Question: I want to clone the psiE gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: C


Answer: D


Question: I want to clone the eutG gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes EcoRI and HindIII?

Answer: B


Answer: D


Answer: C


Question: I want to clone a gene with the sequence ATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTGCGACGGCGATGGCTTCGCTGCTATTGCTGATTAAAATTTTTGCATGGTGGTATACCGGGTCGGTGAGTATTCTCGCCGCGCTGGTGGATTCGCTGGTGGATATCGGCGCGTCGTTGACGAATTTATTGGTGGTGCGATATTCCCTGCAACCTGCCGACGATAATCACTCGTTTGGTCACGGTAAAGCTGAGTCCCTCGCGGCGCTGGCGCAAAGTATGTTTATCTCCGGTTCGGCACTATTCCTGTTTTTGACGGGTATTCAACATCTGATATCTCCAACACCGATGACAGATCCAGGCGTCGGGGTTATCGTGACAATTGTGGCGCTAATTTGTACGATTATCCTTGTCTCGTTTCAGCGTTGGGTGGTGCGGCGGACGCAAAGCCAGGCGGTGCGGGCTGATATGCTACATTACCAGTCTGATGTTATGATGAACGGCGCAATTCTGCTGGCGCTGGGGTTGTCCTGGTACGGCTGGCATCGCGCCGATGCTCTGTTTGCATTGGGAATCGGCATCTATATTTTATATAGCGCGTTACGCATGGGATATGAGGCGGTACAGTCATTACTGGATCGCGCATTGCCTGATGAGGAACGGCAAGAAATTATTGATATCGTGACTTCCTGGCCGGGTGTTAGCGGCGCTCACGATCTTCGCACGCGGCAGTCAGGGCCGACCCGCTTTATTCAGATTCATTTGGAAATGGAAGACTCTCTGCCTTTGGTTCAGGCACATATGGTGGCGGATCAGGTAGAGCAGGCTATTTTACGGCGTTTTCCGGGATCGGATGTAATTATCCATCAGGACCCCTGTTCCGTCGTACCCAGGGAGGGTAAACGGTCTATGCTTTCATAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SacI and PstI?

Answer: A


Answer: C


Answer: E


Question: What is the percent GC of the DNA sequence GTTCCCGTATCCTCGGTTGTTGTACTGTCGTTCCCGGTCCACCAGACGTTCCGTCGACCCAGAACCACCACGGCCGCGCTAGATTGTCTAACGTGCAGACAACATGCCACGAAAAAGGCGGTGTACTGCCCATCGATAAGTGGTGCTAGTCAAGAAAGTCAGTCCTACAACTGAGTGTACTGTCCCTTTCCGAATACAAGCTAGATGAGCTCCCCCCAGTAAACCTGAAGCCTATATCGATTTAAGTTGTCCTAGGGCTCTCCCTAGATTCAAGCCAAACTTCAATTGATCGATCTGCCGTTAGTTTGCGGCTGCCTAGACCGGAAACGAGGCCGGCCGACAGAGGTGGCTCGATTCGAACCATAGCGGGACGACGACTAGGTTATCGCTACGTAATTGCGATCAGCACTTGCCATGCGATGTGTATATAATACTAGGCTC? Round to the nearest integer.

Answer: D


Question: I want to clone a gene with the sequence ATGGACAGATTCCCACGTTCTGATTCAATCGTACAACCCCGGGCTGGCTTGCAAACTTATATGGCTCAAGTCTATGGCTGGATGACCGTTGGCTTGTTGCTGACCGCATTTGTTGCCTGGTATGCGGCTAATTCCGCGGCCGTGATGGAGCTGTTGTTCACTAACCGTGTCTTTTTAATCGGTCTGATCATCGCGCAATTAGCATTGGTTATTGTGTTATCAGCGATGATTCAAAAGCTGAGCGCAGGTGTAACGACGATGCTCTTTATGCTTTATTCGGCGCTGACGGGTCTTACGCTTTCCAGTATATTCATTGTCTATACCGCTGCTTCTATCGCCAGTACTTTCGTCGTTACTGCCGGGATGTTCGGCGCAATGAGCCTGTACGGTTACACCACGAAGCGCGATTTAAGTGGCTTTGGCAATATGCTGTTTATGGCGTTAATCGGCATTGTGCTGGCATCGCTGGTCAACTTCTGGTTGAAAAGCGAAGCATTGATGTGGGCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SstI and HindIII?

Answer: B


Answer: B


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: A


Question: I want to clone the atpB gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: D


Question: I want to clone the rpmH gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: B


Answer: B


Question: I want to clone the ibpB gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SphI and SstI?

Answer: E


Question: I want to clone the gstB gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: C


Question: I want to clone the mntH gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: B


Question: What is the percent GC of the DNA sequence TAGGGGATTCCCAAACATGCGTATTTTGCGTACCTTATTATGTCTGAATCAATGAAGGGGAGGGTTTTGTGGCCAGACCTTATTCTAGGGAATTGGGATATGAGTTTTGTTACACATTGTCTCATCCACTACAACGGCGTCGACAAAGATCTAGCGCTC? Round to the nearest integer.

Answer: B


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: C


Answer: E


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: E


Question: I want to clone a gene with the sequence ATGGCTGATGAAATTGATGCAATGGCTTTATATCGAGCCTGGCAACAACTGGATAATGGATCATGTGCGCAAATTAGACGTGTTTCAGAACCTGATGAATTACGCGATATCCCTGCGTTTTATAGGCTGGTGCAACCTTTTGGTTGGGAAAACCCACGTCACCAGCAGGCTCTTTTGCGCATGGTGTTTTGCCTGAGCGCAGGAAAGAATGTCATCCGACATCAGGACAAAAAATCGGAGCAAACAACAGGTATCTCGTTGGGAAGAGCTTTAGCCAATAGTGGAAGAATTAACGAGCGCCGTATCTTTCAATTAATTCGGGCTGACAGAACAGCCGATATGGTCCAGTTACGTCGATTACTTACTCACGCCGAACCCGTACTTGACTGGCCATTAATGGCCAGGATGTTGACCTGGTGGGGAAAGCGCGAACGCCAGCAACTTCTGGAAGATTTTGTATTGACCACAAACAAAAATGCGTAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes EcoRI and HindIII?

Answer: D


Answer: D


Answer: C


Question: I want to clone the bglF gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: B


Answer: B


Question: I want to clone the yodC gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: A


Answer: B


Answer: E


Question: I want to clone a gene with the sequence ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGTTGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCTGGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCCAAAGCAGAGTTGCTAACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAATGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAACCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTGGCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCAGGTGGGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAACCACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAATACCGCTGACATTAATATTTGTAGCGTCGAAGATCCAGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCCGCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCCGCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCCACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGCCCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGCCATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAAGTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACAGGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCGTACTGGGGATGCCGCATGGCGAGTAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SstI and SalI?

Answer: E


Answer: C


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: B


Question: I want to clone the arcA gene from E. coli into the plasmid pUC19. I have the following primers: GCATGCATGCAGACCCCGCACATTCTTAT, CCCGGGGCTTCTGCGGTGATCTGGAAGATTAA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: E


Question: I want to clone the ftsW gene from E. coli into the plasmid pUC19. I have the following primers: GAATTCATGCGTTTATCTCTCCCTCGCCTGA, TCTAGAAGGCGTTTGTACGAGGTTCACGATGA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: B


Answer: E


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: E


Question: I want to clone the napF gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: D


Answer: A


Question: How many open reading frames that encode proteins greater than 12 AAs in length are in the DNA sequence TCATCCTGCACATGTATGCTGTATGCGCGAATGGCAATAGTCTGTAGGTCCTTGTGTATGTTTAGCCATGATGCGCAGATGCTGGAGGGGCGTTGGACTAAGGTTCAAAGGACACTGTGTCATCTCCGTTTCGTGCTGACAGTTTTCTTGAGAAAAGTCATTATCCATACGAATCATCGTAGACATTCCCCCCGAGGCCGTATTCCCCACCACCTACTACATCTTAGGAGGTCGGTGGCTCCGGGAGCACGTTATCTCCAGGCACGTTCACAGCATCTCGAGCGGTTAAAATATCGGAGAATCACACGTTTACCTCGCTCTTCCTCTCTCGCACAGACACTCTCGCTCAACGGGGAGTCACGCCAC?

Answer: B


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: D


Question: I want to clone the rpmJ gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: D


Answer: E


Question: I want to clone the yihI gene from E. coli into the plasmid pUC19. I have the following primers: GGTACCATGAAACCATCATCTTCAAACTCACGC, CTGCAGCGTCTGCTGCGGGGCAACTAA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: D


Question: I want to clone the ndh gene from E. coli into the plasmid pUC19. I have the following primers: GAATTCTTGACTACGCCATTGAAAAAGATTGTGA, GGTACCTATCCGTCCGCGTTTGAAGTTGCATTAA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: C


Question: I want to clone the speFL gene from E. coli into the plasmid pUC19. I have the following primers: GTCGACATGGAAAATAACAGCCGCACTATGCCCC, GAATTCGACTTTCACTTCTTCAATGCCCGTTAG. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: D


Question: I want to clone the torD gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: B


Answer: C


Question: I want to clone the mlaE gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: B


Question: What is the expected amplicon length if I used the primer pair ATGTTGCGTTACGGTGCTTCCG,TTGGTGCAGGCGGGTGCCTACTC in a PCR from the following template: GGCCCCGCATACCGCTCTTTGGCCCACCATACCGTAGCCGCACGCTACCCAGCCGAATGTTGCGTTACGGTGCTTCCGGTTATCGAAGAACATAGGGGCAGCTCTGAGTGCGCGTGATCTGTGAGAATCCGCAGACACGGCCAGTCACCAGGGTAGACCCTACTGTGGACCTGCCCCATGGTGTGTTACACTAATGATACAGTAATTGGTCGACTGATTGACTCTGTGGATACAAATCACGTGCCCCTCGCCACGCATAGGTCTTATAGTTGATCAGTGGTTGTGTCCACCCGCGATAGCACCCAGGGCCTCGGTTTCTTTCTCTGTACTATCTAAATCGGAGCGATACACGATATATGATAAAGAGAAAATCAGGTGCACCGGCTTTTGTAAAAGTGTCACTGTAGGCCCCACACCCACACACTTTTGACGTACGAGGAACGAACGACGATAGCACCATGGACTCGGCGTACTATGTCCGAGATCAGACTAATGTTGCGCCTGTTGTCTGACAGGCTTTTCGTCTCTTGTCACGAGATACACGCATTCGTTAGTGCCTAAAGACACGCCTGGGAATGTGCGATTTAGGCTAGACACTTCATGTGGGCGTACCGTGTAGACTGCTTTCAAGCTCGGTGGGATCATAGTATGTCCAAATCGCGTTCAATGGTCAGATGTTAGTGTGACCAGTTATTTATATGGCCAATGTCTAGGAAGAGTAGGCACCCGCCTGCACCAAAACTGACGTTTCGCGACCGTCGGAGAGCGTGGACCTTGCTGAGCAACCCCAACCCAAATCCAGGTGTGCGCCCGTCTCAGCAACGGGTCTACCGAAGTATCTTGTTATTTGAACGAAAACCGGTACATGAGAAGCGGCTTCACAGAAGCTAAGTTATCGGTAAACCAGTTAGCCCTCCGACACGAATGCGTCCCTGAGCCCAACTAATATCGTCGATTATAACTAAGGACTAATATTGAGCTTGGGTCACAAGTTTCGTGG?

Answer: A


Question: I want to clone a gene with the sequence ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGATGAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAGTTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAAGAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAGTCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCGCTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTCAACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAAGGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTCGGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTTGTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACCACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCATAGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTTGCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATGTTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCGATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATGGACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATTGGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAGTATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTATGTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCAGGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCGTGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAACTGCTGTCTAACTTCTTCGCCCAGACCGAAGCGCTGGCGTTTGGTAAATCCCGCGAAGTGGTTGAGCAGGAATATCGTGATCAGGGTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTCAAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTCAGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGATCCTGAACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATTCTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes KpnI and SstI?

Answer: A


Question: What is the percent GC of the DNA sequence TCATATTCTGGATGACCCCGGTGTAAGGAAGAGACCAGCTACCGACACGCGATAAAGGCGTACTAAGGTCGTCCTAGGCTTACCCTAGCACACCACCGCTAATGCCCATGTGTTACAAACCCCCTAACATGCGTAAGCATACACTAAAAGGATGTTGTGGCTTGCCACATGTTGCAAAGCACAATCATGTTAGGGGGGCCAATCCAGGTATTGCTTCACCCACGAAAGCAAGACCAAGGGCTAGTGAGAGAACGGCAAACACATGCGTCTGGGAAGCAAGCGACAGCTATGAGACCCGCGAAGGTTATAGATCGTCTGGCGACGGATGGTCTCGCCCCCTAGAAAGGCGGTATATATGAAGGTCTGGTTTTTTTACCATAAGACGCGAAACTGGTGACGGAACACCGAGGTAAATCGTTGGGGCACTTCTTAGTGCCGTGAATGAGTAACCCGGTCTTACCCTGGGTGTCGCAAACGACTGTTCCCTTTGTACGAAGGATTTACTATGACGAGGATTGCACACCAGCTCATTTATTTCATACAGAG? Round to the nearest integer.

Answer: E


Question: I want to clone the ycbJ gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: B


Answer: B


Answer: B


Answer: E


Answer: B


Question: I want to clone a gene with the sequence ATGACGCAATTTGCATTTGTGTTCCCTGGACAGGGTTCTCAAACCGTTGGAATGCTGGCTGATATGGCGGCGAGCTATCCAATTGTCGAAGAAACGTTTGCTGAAGCTTCTGCGGCGCTGGGCTACGACCTGTGGGCGCTGACCCAGCAGGGGCCAGCTGAAGAACTGAATAAAACCTGGCAAACTCAGCCTGCGCTGTTGACTGCATCTGTTGCGCTGTATCGCGTATGGCAGCAGCAGGGCGGTAAAGCACCGGCAATGATGGCCGGTCACAGCCTGGGGGAATACTCCGCGCTGGTTTGCGCTGGTGTGATTGATTTCGCTGATGCGGTGCGTCTGGTTGAGATGCGCGGCAAGTTCATGCAAGAAGCCGTACCGGAAGGCACGGGCGCTATGGCGGCAATCATCGGTCTGGATGATGCGTCTATTGCGAAAGCGTGTGAAGAAGCTGCAGAAGGTCAGGTCGTTTCTCCGGTAAACTTTAACTCTCCGGGACAGGTGGTTATTGCCGGTCATAAAGAAGCGGTTGAGCGTGCTGGCGCTGCCTGTAAAGCGGCGGGCGCAAAACGCGCGCTGCCGTTACCAGTGAGCGTACCGTCTCACTGTGCGCTGATGAAACCAGCAGCCGACAAACTGGCAGTAGAATTAGCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SacI and SmaI?

Answer: A


Question: I want to clone the flgH gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SphI and SmaI?

Answer: A


Answer: D


Question: I want to clone the yeeA gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: C


Question: I want to clone the yhdP gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: C


Question: What is the expected amplicon length if I used the primer pair TGTCTATTCCACTTGTGCAACAGATA,CCGCTTGTACTTCAAGTCCG in a PCR from the following template: CGGCCGGTTCTCGAAGGAAAGGATCCATGTGGCGATGTCACTAAGCAGCAACGGGCCGCCCTTACCCGAGCCTTGCTCACTCTCAATCGGTGTACCTTCGCCCGCCGGTACAGGAGTATTTCCAATCCATAGCAATTCCATGTCAAGGAACGTTTTGCAGTAAACGTACGATTGGAGCACCCGGCGTAGTAGCGATTAGGCCCGAATGCACTGGGCGAGCATAATTTCGCTTTAATGTCGACGGGGAGAGTCAGGGTCAGGGGGCGCGACCTTACAAGTTTATGACCCAGTAGCGAACGGACGAACTATGACTAAGTGGATTTCAAACATACCAGACCTAAATCGACGCACGGTCCTACTCCTACAAGTGCGGGGAATCTGTAAGTGTTTTTGGGCCCCCAACGATTACTAACTGTCTATTCCACTTGTGCAACAGATAGAGTTAATTCATATGCTCCAATCTCCGGACACGAGAAACCCGGATTAGGCTCTTTTACTATCCGCAAGCATTGAGCTGTAAATTAATGGAGGAAGAGTGCCTCACGAACTCCCCTCGTTGCACCTTCCCGATCATTGCTTAAATGCTGCCAAGGTATCCAGTCCGAGTGACAATTGGCTTTGTTCCAGATAGCGGTCCTGCTCATAGTTCGTTGGTGCAGCTTTTGCGGGCTGAGGAGACACCGAATTGGCGAATTAAGAAGTGCCTGCGGTATCCGATCGCTATCAAGCATAAAGTTCGGACTTGAAGTACAAGCGGAATGTAGGGATGGAGACAGCCAGTGGAATCACGATTCAAAGTGTCGCAGAGCAACGCCTCGCGAAAGGTCTTTCTGGCATTGGTCGTATGGACGTCCAGGTAGCCACAGCTCTAAAAAAGCCTATAACTGGAGCTGCAACGCTATTAGCGCGGAGGCTGACCGGGTGACACCGGCGCATAAGAAGTGTGATGAGCAAAAAGGACTAAGCTAAAACGTGTCGAGCAGGGTGGTTAAATTAGCGT?

Answer: E


Question: What is the expected amplicon length if I used the primer pair GAAAATAGAAAGATTGGGTACCGGGTAG,CCTATCGGAGCTATGTGGACACGCGA in a PCR from the following template: CCGTCGGTTAGTACGGTTTCCATTACTCAAGAAGCCATCGGTCTCATAATGAACACTAGGTATCTACACTCCTCTGCATGCCGAGACTACTGGATATACTCTTTATGATCCCCACGTAAAATCATCGACAATATCGGCAGCACTGAGGGCCGTAATGGTAGCTCTGTAAAAATCGCCGGGATCTATAGGAAGAACCTTGTTTGGACGTTGTTTCGAGTAACGGGAAATCACTCAGCTCTCGGATCAGGCCGGCATATAAATTGCAATTGAGGTAAAAATTTCTAGTGTCTCATTGACCTGTATTAAGCACCATTGGACTGTCCACAGATACGCGGGTTCTGCTTCAATCCCCGAGTGAAATGTCTGGGGCCAACTGCGCTCAGCTTATATGGCAAGATGAAAATAGAAAGATTGGGTACCGGGTAGCGTTCCTAAAAAAGCAAACTAGCACCGCACCTAGGTAAGGCACCACGATGCGGGTCCAGATTAATCATGTACTAAGCATCGCCTAATTGCAACAGATACGCCTGGTTAACGTGTCTCACGATCTAGACAGGCCGGTCCCCGACCAACATAACGCATTAGCGATTCCCGTCAACCAACGTATATCTCTTTTTTCGGACCTCGGTACTTATTCTAGCGTAGGGAGGAGGAAGGTTGGCGTCGTACTGTGTTGTGATGCCCGGAAGCTCCCAGGAGTGCCTAACGTTCAACCGCGAGTCGCCAGACCGCCCTTAAAAACTGCGATCTTCTAGGCGCCGCGACCGCATATACTAGTCAACGCACACCCGTCTGGCGTTCCCGGGGCGCAGAGTGTCTAGTTTTTCGCGTGTCCACATAGCTCCGATAGGGAAGATCCACGAGGTCTAGTTGACGCAACACGCAAGGGAAAGGCGAGCGGTGCTAAATCAACAGAATGTAGCGCGACCCCACACACTAACTGACAACTAACCCGTGATCATAAAGAACCTGTGCGCGTGCAGCCGCTCCCGCGGCTGAA?

Answer: E


Answer: A


Question: I want to clone the malT gene from E. coli into the plasmid pUC19. I have the following primers: GGATCCATGCTGATTCCGTCAAAACTAAGTCG, CCCGGGGATGATGGGGTACGGCGTGTAA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: B


Answer: E


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: B


Answer: C


Question: How many open reading frames that encode proteins greater than 13 AAs in length are in the DNA sequence AATGGGACCCTAGGAGAATTCTAGGATTTTGAGCGTTTCCTCCTAGAAGATTTTGGTGACAAATATCAGTTGCTATAACCGAATACCAATATTTACCAACCAGCAAAGGGCATTTTACGGCATGGGTTCAGATAAGCACAGTGGGATAATTGGCAGGGATTCATGATTGTCTGGGGCATACCATTACCATTGCACTAACTCGCGCGTCTTCTCCGACCGAGGCCGTCGCAGGTGATTTGCTTACTGGGCTAATACAACGGTGAGCGTGTACTTCCTCTAAGCACAGGCGTCCCTTATCAGCGCTAATAAACTGTGTGCTGTGCCTGGGCGACTGAGTGCATTCAGGGACTCGTGTGACCTTCCACGAGAAGGGGTGCTCAACTTGAAATATGGTAGTTTACATAGCCATGGCCCCCCACAGTGATAGAATAGAATCTACGAAAGAACATGGAGACTTTTGCGCTGCGACCACTCCGAATACGAACAAAAGCTCCAGAGACAAAGCTACGTCCTGCCTTTCTGGCTTGATTCAGGTTAATAATCTCCGCCTTCTGAAAGATAACTGGTATCAACCTGTATTTACTATCCACGCGCTCTAGAGAATCCAATCTAGTGACGGCCAATGAATCACTTCCATTTTGTTCTATATTTGTTGCAAACGCGCGACCTGGGTTCCAGTTCGTTAGCTGCGGTATCAACTGCTCTCCTCGGGAAAAGCACGAGGAAGATGCGATAGTACAGGCACTCGGAGCGTTTCCGACTGTTTTCTTTCAGCCTCGAGCTGTCATCTAGTTGTGGTCATACAGATGCATTATCTCTACTCACTTAATGGGACCTGCCGAACAACCCTAGGCGGTGCGCCGGGTTGATGATCCAATTTCCGGGGTCATACAGTTCTGTCGTGCTTATACCAGACTAATGTCCGGGACGGCTATCAGCCTTGATGTATCTCGGAAGAAGAGTCTCTCTGCGAGCTCGTCGCATCTAATCGTCCACGTTCACGGCGCCCCTGAGTGGGAAAAGCCGGAAACATAG?

Answer: E


Question: I want to clone the ycbJ gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: B


Question: What is the expected amplicon length if I used the primer pair GCGATCCCGCGATGTTAGGCTAC,TGGATTTATGCTATGGGCCTTATTACA in a PCR from the following template: CGTAGGGGAGGCTCTAACAGCATTTGACTCGTCAACCCCGTTATGATCGGTACGGTTTAACTCCTATGTCTATATTGCGGCGCCAAAGACCAGTCTCCCTGGCCATACGGATCTCTCCCCAGCAGCTGTTATCGTATACTTGAGCACGCTTCGCTAGTGAGCGTTGGCTGCTTATCATCGATTTGGGCATTAGACAGGTAAAGCAATCGATGCAATTTTCGAGTTCTTATTGTCTTGCTCTAGATTCTACCAGCCGTTCATCCGCAAAAGGTTGGTTCATAGTTGGCGCCACCTATCAATCAGCCTGCACTTTACAAGCGACCTTGTATCAGTCAAGGAGCTGGAATATTCTTAAACGGCAGATTTCTAAAACGGAATACGTCCCCCATTATTAGTGCTAGATAATCGTCTGTGTGCGATCCCGCGATGTTAGGCTACAACTTGCCTGACGCGATTTCACAAACGTCTTTCCGGAAGTGTTGCTCAAACTTTTACTTCCGAGTTTGCGATATATAGGGGGAGTATACGACGATCGCCAATTCAGACCGACTGTCGAATCAGGGTGGACGTACGCCCTACCTGCTTCTCAAGCCCTCTTTACACTAGCAGTTGGGACGAGATGGACAAGACAAAATTTTATTAAGCACCCGTCGCCATCACGTGTAATAAGTGATACTGCCTCTTCCGGGTTGGGACCTACTCTTAGATCGCAGCGATACCTCTGGCTGTAGATCCATGAGGCGGAAGGCCTGAATCGAAAACGGGTCGTGCACATCGGCTTACGAGGCTTCCGAGCTCCACCGTGCGATGCCGTGATAGTAACGCTACCCGCTTGTGTAGTGTTCACCAATGCGTATTTTTCTCCGGTGAAATGCGAAAGCACATCTTAGCTCTGTAATAAGGCCCATAGCATAAATCCAACTAGTCCGATCATGCTGTGGCCACCAACACTGGCCTCGTACTTCCCGTGGCCCGCGCGCTTGGAACAGTTGTTGCGGTC?

Answer: D


Question: I want to clone the afuC gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SalI and EcoRI?

Answer: B


Question: I want to clone the cyoC gene from E. coli into the plasmid pUC19. I have the following primers: CCCGGGATGGCAACTGATACTTTGACGCACG, GTCGACTGTTTATCTGATGGGGGCGATGTAA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: C


Question: I want to clone a gene with the sequence GTGGCAGTCGCCATGGATAATGCAATTTTAGAAAACATCTTGCGGCAAGTGCGGCCGCTCATTGGTCAGGGTAAAGTCGCGGATTATATTCCGGCGCTGGCTACAGTAGACGGTTCCCGATTGGGGATTGCTATCTGTACCGTTGACGGACAGCTTTTTCAGGCCGGAGACGCGCAAGAACGTTTTTCCATTCAGTCTATTTCCAAAGTGCTGAGTCTCGTTGTCGCCATGCGTCATTACTCCGAAGAGGAAATCTGGCAACGCGTCGGCAAAGATCCGTCTGGATCACCGTTCAATTCCTTAGTGCAACTGGAAATGGAGCAGGGTATACCGCGTAATCCGTTCATTAATGCCGGTGCGCTGGTGGTCTGCGATATGTTGCAAGGGCGATTAAGCGCACCACGGCAACGTATGCTGGAAGTCGTGCGCGGCTTAAGCGGTGTGTCTGATATTTCCTACGATACGGTGGTAGCGCGTTCCGAATTTGAACATTCCGCGCGAAATGCGGCTATCGCCTGGCTGATGAAGTCGTTTGGCAATTTCCATCATGACGTGACAACCGTTCTGCAAAACTACTTTCATTACTGCGCTCTGAAAATGAGCTGTGTAGAGCTGGCCCGGACGTTTGTCTTTCTGGCTAATCAGGGGAAAGCTATTCATATTGATGAACCAGTGGTGACGCCAATGCAGGCGCGGCAAATTAACGCGCTGATGGCGACCAGTGGTATGTACCAGAACGCGGGGGAGTTTGCCTGGCGGGTGGGGCTACCGGCGAAATCTGGCGTTGGTGGCGGTATTGTGGCGATTGTTCCGCATGAAATGGCCATCGCTGTCTGGAGTCCGGAACTGGATGATGCAGGTAACTCGCTTGCGGGTATTGCCGTTCTTGAACAATTGACGAAACAGTTAGGGCGTTCGGTTTATTAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SacI and SalI?

Answer: B


Question: What is the expected amplicon length if I used the primer pair GCTAGCTGCCGGTACTTCGAGTATAG,ATCGGGCTAACCTACGGAAAGT in a PCR from the following template: AGTCACATACACATGTATGCACATCTACACATCTTAAAACGGTTTTCTCATCGTGTGCACGCGTGCACAAACCCTCTGCATCAGCGGACGTGAAGAGACTCTGAGATACTTACGTGTTGACTTAGAGTCCCCAGGGAGCCGAAGGACTTACACAATCCGGCCCCCCTGTAGATTTGCTTAAGCGTTTATACCACCAGACCAGCCCTGGGCTTTGGGAGCCTCGTCCTGCGGGCGCTTTTTACCGCATCGTACTAGGCGATCACCGGCTAAGCTAGCTGCCGGTACTTCGAGTATAGGCTAGCTGCAAGTATATTATCGTAATCGGCGTATGACATATATATGGCGACTTTTGAAAAGAGTCAGGGACGGTGGTCGAAAGTAGTACGGGAACCCAATCTGGCAGTTCGCCTTACTTTATAGTATAAGGATTCCCGAGGACTGGCCGCCATTAATAGGACTGGCATTCAACGGCAGCCCACGCATCCCACTTGGTATAAGCTAGTGCCATGAAGAGAGCTGAGTATCGGAAGCCTTAGAGCATACCGTTATAACCTTTAACACACGCAACAGATCGGGGTATAACAGCACCGATAGGAAATACATTTGTGACGGATCAGTGGTGGGCAATTAGTGCTGCTTAAAGAGTCATAGGGGTGTTAGGCTTCGAGGGTCGGCGCTACATGTCACGTAATTGGGAGGGTTGTCGCGTGAAGCGTTTTGGTGGCCTCCGCGAAGCCCTTCTGGGAATGTCGGTTAACGTGAGGGCCTGTATTGCATTACTTGGGGTCGGCGATCACACCGCAACGCCTGCCGGCCCCAGACCAGGCATGTCTTAAATCTTCTTTTGGCACATGTTTTAATGTCACGTAATGAGCATTACACTTTCCGTAGGTTAGCCCGATGGCTACGACATTCGGTCTCTAAATACAATATCATGTCAGTCGCTATAGGGAGCCCAAACTTATAATATCCGCTACGTCAAAGGTCTAAAGGGAGCTAA?

Answer: C


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: B


Question: What is the expected amplicon length if I used the primer pair TTTCCTTCCTTGAATGAGATTTTAT,TTAAGACATCGTTTGGGCATCTACGG in a PCR from the following template: TTGATTCAAGACCTATCGCTTAGCGTCGGAACTCACTTGTGCAGAGTAGGCCGCGGGACGTGATTGAATTCCGGACTGCCTTGATAGGAGCATCCCGGTTTCTGCCGAATAGCGCAGGCATAATGTGTTCGTAAACGCCATGAGCGCTTAGCGTACGCATGGGGGAAGAGAATGGAAATTTAGTCTCGCTCCACTCGTGATAAATGTTTCTTGGCTAGGAGTTTCTGTCAGAACCTCTGGCGGACTTTATTTCCTTCCTTGAATGAGATTTTATCTTGACCTCCCCTCTATCCGGGGCAGACCCTCCGAAGACCGTCACGCACCACAGAGAACACCAGGGCACAGGACACGCGCGGTTGTTCGTCAGAATGGAACAACGCTAGCGTCTTGTCCCTGGTATCTAGCTACTTGCCCAAGAGGCGCTTCGTGCTTCAGATGTAGTCTTCGGGTACTCTAGCTATTTACCCGACGTTAAATACTGCCCGTCCGGCTCGGCTACCGGGAGGTACTTCGATTACTCGTATCCTGCCTCAGCTTTGATAAGTCGAATTAAGAGTTACCTACCGGAACCCGGTACTACGGCACGGGGCAGAAGAGCACGTAGAAATGGTCGAATGCACTAAGGTTGAATGAGCCGCCCCCGTAGATGCCCAAACGATGTCTTAACATTCTACTCCGGCTATAGTGCACTAGTACATCTTTTGCGTGTGGCTGACGTATGCTTCGTAGCGGATCTGGCGGAGGAGGGCAAAGGTCAGATGCGGATTGACGATCTTAGACTTGAAGCGGCTTGGATTCGTGACAGTTTGCGGCTAGTCGACTGTGCCAGGCTGGCCAAATCCTAGCCGAGCAAATGACCGCAAGACGTTGCCGGAGTCAGTACGAAACTGGCGTAGTATCGACCTAACATACGTCTATTTGGCCTCTCTCTTCTAGGGGGACCCATTGTCTTGGGCCTTTCTACATCCCTGTATGAGTTGTGAAACATCGGCACATGCCG?

Answer: E


Answer: E


Answer: E


Answer: D


Answer: B


Answer: A


Question: I want to clone a gene with the sequence ATGAAACCTCGTCAGCGTCAGGCCGCCATTCTGGAGTATCTGCAAAAGCAGGGTAAATGCTCGGTTGAAGAATTGGCGCAATACTTTGACACCACAGGCACAACCATTCGCAAAGATCTGGTCATTCTGGAACATGCCGGAACCGTCATTCGTACTTATGGCGGAGTGGTGTTGAATAAAGAGGAATCCGATCCGCCTATCGATCATAAAACACTCATCAACACCCACAAGAAAGAGCTGATTGCAGAAGCTGCCGTTAGTTTTATCCATGATGGCGATTCGATCATTCTTGATGCTGGCAGTACCGTTTTGCAGATGGTTCCCCTGCTCTCGCGCTTTAATAACATCACGGTGATGACCAACAGCCTGCATATCGTCAATGCGCTATCCGAACTGGATAACGAACAAACTATCCTGATGCCAGGCGGAACGTTTCGCAAAAAATCGGCCTCATTTCACGGGCAACTGGCAGAGAATGCCTTCGAGCATTTCACCTTCGATAAATTGTTTATGGGCACCGACGGCATCGATCTCAATGCGGGCGTAACCACCTTTAACGAGGTTTATACCGTCAGTAAGGCAATGTGCAATGCCGCGCGCGAAGTGATTTTGATGGCGGACTCATCAAAGTTTGGCCGTAAAAGCCCCAACGTAGTTTGCAGTCTTGAAAGCGTCGATAAGCTGATTACCGACGCAGGTATCGATCCGGCGTTTCGTCAGGCGCTGGAAGAGAAAGGGATCGATGTGATCATAACCGGAGAGAGCAATGAGTGA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SacI and EcoRI?

Answer: E


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: E


Question: I want to clone the yfeC gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: B


Question: I want to clone the pgpB gene from E. coli into the plasmid pUC19. I have the following primers: TCTAGAATGCGTTCGATTGCCAGACGTAC, CCCGGGGCGCAACGAGAACAAGAAAGTTAA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: D


Answer: C


Answer: D


Answer: B


Question: I want to clone the eutK gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: A


Question: What is the percent GC of the DNA sequence CATAACAGAAACATGCCACGTGCAAGAGTCAAGAGCAGAATTGTCAGCGGAAGCTCGACAGAATACATACGAGGTGGTTAAAACACCTGTAGCATGAGTGGTGACCCAGTCGAGGATAAGACATCCATTCCTGTGACATCAGCAGTTCGCATGCATATGATC? Round to the nearest integer.

Answer: E


Answer: D


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: A


Answer: A


Question: I want to clone the rodZ gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SalI and HindIII?

Answer: D


Question: What is the expected amplicon length if I used the primer pair TCATCGCAGGCAGTGACGGCTA,ACCAGGCGCTTAATCAGCAGA in a PCR from the following template: TTTTAGTAACACATACCCCGTGAGTACTCTAAGGGCCGGCCTAGTTGAGACGCTGTTCATCTCAGTGCAGAACGTTTTTGTGACAGACTTTATTAAAAGATAAAGCGATTGAACTCCTGACGTTATTAGACTTCTGCCGCGGTTCTACGGACTACTCCAAGATACTTCAGCGCGCATGAAGGTACGCCCTCTATGCCCTAGCGAGACTTCTTGTCGAAAAGGCGATAATTTGTGTGCCTGGGACGACTGCAGAACATGCGATGCTTTCGGACGCAGCGTTATATATCTGAGTGCGCTGGGCGGTTGAGTCTCCGATGCCTAATATCGGAAAAACTCAGCCCAGGTTAATAAAATATATTGTGAACTAAGCTTCTAGGGCGCTATAGGTTCGACTCGAAGACCCCAATTGGTTTCTGCTACGCACAGCCGGGAGCTCAAACCGCAATATCGTGTGATTCATCGCAGGCAGTGACGGCTAATTGGTACTAAATTCTATAGAGAAGACTTTAAGAGGCGGCGGTAACGGAAGGGGATCGGATCAGTTTACCCTTTGCTAGTCTGTAACCAACTTACAAGTGGTAATATCATTGTCTACCGCTAGTGAAATTTTGCATCTGCTGATTAAGCGCCTGGTGCTGTTCACCGGAAGACACGTTTCGCCTCCGTTGGCGCCTGCTTTGTACGCACCTATTGACCGGTTGATGGCTAATACTAATCTGCCTGAATAAGCGGCAAAGGGATAACCATCTTCAATCGTCCCGGTGTGAGGTGCACACAGCCGCAAGTCGGGCGCACTACCCTTTTACAGGCATAACGCAAGGAGTGTGTGTGGCAGCGACCGTTATTCCGTTACTAGAGTGTGCTTCGGGTTCTTCGATTCTTTCAACTTCGGGCTTGAGCGGGATGATGTTGCAGAATGCCGTTCGCATTTTTGTCGTAATAAGACTACGCCATCCGATGATTTAACCTCCCTCCCCCCCGAGTTATCGCTCTAAAGGCG?

Answer: B


Question: I want to clone the yfdP gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: E


Answer: E


Answer: B


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: E


Question: What is the expected amplicon length if I used the primer pair ACAAGCTCCCGGGACCCAGGTACAG,AACAGCAGGCTTTCATACGGAT in a PCR from the following template: GAGAACTTATTAATTCGCCCGCCGCCCACCCAGTCGCAACCGCAAGCTGATAGTCTAATTAGACATCATGATTTGGGCTTCGCGTTCGATCAAGAACAAAAATCAAAGCACTAGCTGGTCAGGTCTTTCCGCTGCTAATTCTCCCCGGGACTGGTTTCGATGAAGCAGCATCCACGTAGACACTCCGGGATGGTCAGTAGCCGCACTGTGGGTTTGTTTGTTTTGAGCAATGTTGGCCTTGTGGAACATTTGCTCTATCTTGGGGAACAATCCTACAGGTAACCGATTTTGGGAATAATCGTAGCTCCACAGGCCGTTCCTTATCCATCCAAGCTGATTATATCGGCAAAGAGTTTCTCTTCCCTTCCAGCCTACTACCTTTAGCAGCGTTCGGGAGCACTGTGTCTCTTTTACAAGCTCCCGGGACCCAGGTACAGGACACGTCTCAAGGTCATTAAGGGCTTCTTCCCTACGTGTGACCGGCCTGGGCTGAAGGATTTTTCGTTTGCTAAGCAAGAACATGAGAGAAGTCCGGGTTCGCTGTAACCCTATTTGTGTTGCATCTTCGGTGCGTATCACGGCGTCAATTATCCATCTTGCGCGATCAATTCCGAAAAGGAGTTTTCTTTGTATCCGTATGAAAGCCTGCTGTTTTCGGTGGTCGCACCCCAAAGGCCATAGGCACACCCAATGCGCGAGACTTGTTAGTAAACTTGCGCCTTTTTCTGCTTTGTTCGTCTAAGTCGACCGAGCCGTCTTAAAATGCCAGCATCAGGGCTGGGTACCCTACGCATAGTGCCCGGCGGCTGTCCCTACCGGTCATCAACTATAGCTATCTCGATTCTACGTTAAGGCAACGACCCCCTGCTGCCATCTACTTACCTCAGACACTGCCACTCTTTTTAACTTCTGCCGCGGGACAAACCCGGTAACCTTGAGACCCGCCTTCCGTGGCAGAATCTTAAGAACTCCTCAGTGGGTCTTCCTAGTGTTTGTGCCA?

Answer: C


Question: How many open reading frames that encode proteins greater than 17 AAs in length are in the DNA sequence GATACATCGGAGTACGGGACAGCTTTAGAAGCAGCTATCCGCGAGCAACGAGCAGCGGCAGTGCTGGGAAGGGCAACAAGCGGGCCGAGGGTCACGGTTGTCAGTGATATATGCTAGCCACTCTGCACAACCCGTCATGGAAAGGCAAACATGCCGTAATGCTGAAGGCGATTAGAATATTGCTCTTTTATTACGTGGTCGCGAAATTTGTGCAAGACCTAGAGAACGCGAGTTAAACCAGTTATGCTGCTCAAGTTGTTCATTAAACCG?

Answer: B


Answer: A


Answer: B


Question: I want to clone the flhD gene from E. coli into the plasmid pUC19. I have the following primers: CCCGGGATGCATACCTCCGAGTTGCTGAAACACA, CTGCAGGCTGCGCAAGAAAAGGGCCTGA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: C


Answer: F


Question: I want to clone the iraD gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: E


Answer: E


Answer: B


Question: I want to clone the yjiA gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SalI and SmaI?

Answer: E


Answer: B


Question: I want to clone the recX gene from E. coli into the plasmid pUC19. I have the following primers: CCCGGGATGACAGAATCAACATCCCGTCGCC, GTCGACATTTGGCGAAATTTTGCCGACTGA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: B


Question: I want to clone the menA gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: B


Question: I want to clone a gene with the sequence ATGGTTCAGAAGCCCCTCATTAAGCAGGGATATTCACTGGCAGAGGAAATAGCCAACAGCGTCAGTCACGGCATTGGGTTGGTGTTTGGTATCGTTGGGCTGGTGTTGCTACTGGTTCAGGCGGTGGATCTTAATGCCAGCGCCACAGCGATAACCAGCTACAGCCTCTATGGCGGCAGTATGATCCTGCTGTTCCTCGCTTCGACGCTCTATCACGCCATTCCTCATCAACGGGCAAAAATGTGGCTGAAGAAATTTGACCATTGCGCTATTTACCTGTTGATTGCCGGAACCTACACGCCGTTTTTGCTGGTGGGGCTGGATTCTCCGTTAGCGCGCGGGTTGATGATTGTTATCTGGAGCCTGGCATTGCTGGGTATTCTGTTTAAACTGACCATCGCGCACCGATTCAAAATTTTATCTCTGGTGACCTATCTGGCGATGGGCTGGCTGTCGCTGGTGGTAATTTATGAAATGGCAGTTAAGCTCGCGGCGGGCAGCGTTACCTTACTGGCGGTAGGCGGCGTGGTTTATTCGCTCGGGGTGATTTTCTACGTCTGCAAACGCATTCCATACAACCATGCCATCTGGCACGGCTTCGTGCTCGGCGGTAGTGTGTGCCACTTTCTGGCGATCTATTTGTATATTGGGCAGGCGTAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SphI and HindIII?

Answer: D


Question: What is the expected amplicon length if I used the primer pair CAGTTCCGATTAGAAGTTCACT,TTCTCTCGAACGTACCCCGCTATGCAA in a PCR from the following template: CGCGTCTTCATTATTAAACGCTTCTGCGACGGAATAACCTGCTTACCCGCGGACTCCGCTGATATGATGCCTGTTGTAAGCAATCTCAAGACAAAAGGCGTACGGGCGTAGCGTGCGAGTACATTGGGTACGACTTTAAGGCAGTGGCTAAGCTACCCTTTCAATACGATCCGGCGTTTAGATTGCGTAAAGGCTAGTGGATTGCCCTACCGTAAAAACAGATATCCAGTTCCGATTAGAAGTTCACTTGTTTGGACGTAGGTAGTCCGAGAACTGTGATCGCGCAGATATACGAGTTACTGTTCATACCGCCTGACTAGCGGACTATCTGAGCCTAAGAGCCGTTCAAGACCGTAGTAGCCACCCGTCAGCGGACACCTGCCCCTTTGAGTTGGAGACGGAAGGCAGAAAGAATAATAGTCGCAGGAAATCCGAGAACATGGTTGCTTGGATCTCGTAAGGGCGACGGCCCCAGAGAACGCTAAAGGGTGTACACAGCTGTGATGATTAAGCCCGCAGAGCCGTACCCGTTTGTCATTCTATCCTGGTGCGTTTCTAGCTTGGGTTGACAACTCTGAGGGCAGCCACCGCTGTTGCATGGCAGCGCCTATCCACTCCACTGTCTACCCTCCGTACGATGTATGACTTTCTTCAGCTTATTTACACGCGAGTTCCCTACCTAAATAACACTGCAGTAGCCAATCAGAGGATCTCGCATACTCACCAATAATCTCTCAGCCCCTTAAGTTAGGAAGGTGGGGATAGCATTTGAGCTAACCATGTAAAAATGGCTCCCGTCTATGTGAGAACATCCTTGTCGGGGCAAGCTATACACAAAGGGAGGATCTCTGCAACAATGCGTGTATCACTGATTCCAAGCCACAATATAGCCTACCTCTTGGCGTTCCTGGCTGGAACTAAGTATATTGCATAGCGGGGTACGTTCGAGAGAACGCTCTTAAAGAAAGTGTGAACGAGCCTTGGAGTACTTACGGGGTGA?

Answer: C


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: C


Question: I want to clone the hslO gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: E


Question: I want to clone the nrdE gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes XmaI and XbaI?

Answer: C


Question: I want to clone the rsxD gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: D


Answer: E


Answer: D


Question: I want to clone the waaA gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: C


Question: How many open reading frames that encode proteins greater than 17 AAs in length are in the DNA sequence TGGTCTGTTAAGACCACAGGAGTTGGTGCCCGTTTTACGCCGCGGGCTCTGACTCTCAACGTTTGCCCCCCACTGTCCGCCCAGAGGCTGTGTGAGCTTCTTTGATGACGAGGGGAGACCCTTCGACCTTCGGTCTCGAGGGGAACGGGGCACTAGCCCGAGAGTTTCTGTACGCATGCCCGTGGTCAGGGTTCTAGTTTTTGAGACTGTGTCCTACTGGGCCCGCACGGGTAGCTATCCTTTGAGAGCCAAGGCTCTAAGGGTTGGGTTTCCAGCGTCGCAGCTCTAAGCGCTCGTTCCCATCCTTCAAGCTGATACTGGAGTCTGGCGCCGGGTTTC?

Answer: C


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: E


Question: I want to clone the ubiB gene from E. coli into the plasmid pUC19. I have the following primers: CTGCAGATGACGCCAGGTGAAGTACGGCGCCTAT, GGATCCCGGTTGGCGCAAAACACGCTGA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: E


Answer: D


Answer: B


Question: I want to clone the fixX gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: A


Answer: B


Answer: B


Question: I want to clone the fliO gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes EcoRI and XmaI?

Answer: B


Answer: A


Question: What is the expected amplicon length if I used the primer pair AGGTCGAGGGTCTGAGATAAAGTGCT,ATTCAGTTTGATAACGAACTCTCATG in a PCR from the following template: CCTGCATATCTTGACCTTGCTGCGTAGGTCGAGGGTCTGAGATAAAGTGCTGCACACTAGTGGTAACAAGATGATCGACGCAATGAGGGAGATACAGGGAGCATTCTCTAGTGCAACGCGCCTGTCATTCGGTATAGACAAGTAAAACATGAATCTAGACGAGTGCATCAGCTTGACGACCGAGCGTCCTGTGACGCGCCAGTACGTTCCTCTCCTATATCTCTCAACTGAAACTTGGAAGTCTGCAAAATCCCGCACCGCAAGGCACCTGAACCCCTTGCCGATTATTATGCTCGCCCTTGTGAAAATCTATAACCGATAACGTGGACACAGATCGGCGTGGGCTTGATAGGTCGTCGATAACCCCCGTATAATAAATAGCCATGAAAGGAGGAGCACGGCCTTCTTGACCGTATGCCGCGGGCAAGTTCTTACCTCTTGTTCCGGGCCCTGTGCTGTAACCGATCACCGTATTAAAGAGCTAGCTTATCCTCAAAAGATCCGTCTTTGCTCTGGAACTTGAATCTTGCGTGTGGGGCGAGTCCAGGTGCGATCCTAATTATAGGGCTCACTGGACAGTAGCCTACTATCCACTAAGCAACCGAGATGATTTTAGGATCCGACACTTTACTCAAGAGACTGTGCTAATCCGAGAAATACAGCATGAGAGTTCGTTATCAAACTGAATGCGGGATCTCTTACTGACCTATCGCATAGCAGCGATGGGAACATCATCTCGCCCCTACGCATTCTCTGTCCCATCTGTGTCGCACTCCTAGTAAGGGGGGCTAGCTCCCTTTCAATTAGAGTCTGTGATAGTATCGGTATCATCTTTTCACGAAATTCACTCCGGTGCGATTAATTCCTTTGATCACGCTTGCATTGCTGATACTCCATTGAGGGGATGTGATATGGATCCTTAGCCGAAGACCCGCATTCGTCTTAGGGAGCCAACACGTGTGTCTGACAATAGCGACTGAAGACAGTCTGGGACAACCTA?

Answer: A


Question: I want to clone the yggS gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes KpnI and HindIII?

Answer: A


Question: What is the expected amplicon length if I used the primer pair GCACCTCAGTCCGCATTCTCGC,CCGTGATGGGGAGCTATTACGTCCTCCA in a PCR from the following template: TGTCGAGGCCCTCCTCTCCTGCGACGCCGTATCTTTGATTAGTTCAGTATAACCTGGTGGCTATGGCAGCAGATCATATGGAGGCGGAATCTCGCACGCTTCTGGATCGGGACGCCTTATATCAAAACCTGATACACCATAGAACTCTGCCAAGGCGCCAATATGTTCGAGAAATCAGCAATGGCTATCACGAATTAAAGGGACGGCATCCACTAACAGCTGCCGCCCGTTAGGTCAGTACTTGGAATTGTACTTGACCCATTTGCTCTGTCTGTCGTCCCGCGCCCCCACGTCGCATTTAATGTACTCCCGAAGTCAAAGATCACCATGGGTGTATGCTAAACAGCGCCTCCTACACGCTAAAAGCCCCGTAATAACACAATCATTGGTTTTTCCGGCGCCGGACAGGCCTATGGTCGAGTGTCAGCGAATGGAGCCGTAACCCCAATTTGCATACGCACCTCAGTCCGCATTCTCGCCGGATACGTCCCTTCACGCCGACTTTTCCAACACGACCATGCCATTACGTAAACGTTGAAGGACGCATCGTGCGGTGTAGAAGCGACCGTTCGAGTCACTGTCGGAGAGCCGACTCAAGGTTTATGAATAATGGAGTGGGGTTTTGTTTCGATTTCTGGACGGTGCGGACACGTTTCTGAACGCCTCTGTACGGTATCGCACGAGGTTCGATTTGCAAGAATGTAAATGGAGGACGTAATAGCTCCCCATCACGGGGGGATTAGGTCTAAATTCAGTACGCGAGTTGGTTGGCCAACGGCAGGACTTGTCTTGGTGCCCCAAGTATGTGACCCGGGCGAATCTTAAACTAGGCCGGTAAGCTCTAAACCTTATGGGCGTATGTCTGCATGAAGGTTCCTTATGCGAATAAGCCTGTCCAATCGAGGAAAGTCGACGCCGCGACTGCACCGATCTTCCTGCCACCCAGAGGATCCACGGCCGGAATTATGAGCTATAAACTTCCAACCGTGCTCGTAGTTTG?

Answer: A


Answer: C


Answer: D


Question: What is the expected amplicon length if I used the primer pair CTTTAATCCAACCAATAATCGCAAGA,AGGCTACCATTAGTGCATAC in a PCR from the following template: TGCGTCATGCTGTGAGCAGCTGTCACGAAGGCTGATCAGTTCGGCAATATAAAGTGTCAATCATGAAGAAGGCAGAATGCTGGATCCGTCAGCGCATCTTATCCTTCGTTTCTATAATGTGCCACCACAGCTTTGAGGTTAATTGCTAACATGTCTCGACCTGCCTTATTCTCTACGTGTTTCTACATATCTCCTCTATACTTCTTAACTGGTATCTAACACGTCTCAATGAATCCCGGACAACGAGGGATACTCATTATATAGTGAGAATTAAGTAGGGGAGCATGCACCATGTCAGTAGTTACCCAGCAAACACTTGGATTGTGAGCCCCTTTAATCCAACCAATAATCGCAAGATAACTTATTAGGTCAGCATTCCTGTCCGCTCGGACGACATGCAAGATTACTGCTGGCACTTAATAAGGGAGGGTACACGTGTTGCGCTTCAGTCAGAACCCGTGTGCCGATTAAATATATAGCGGGCAAAATCACCCTGATCTGTTCCCTCGTAGTTTAGGTCCATGCCAGCATCTGACTTTTTCGGCTTTTCTTCAGTTCCCTGTGGCTGTGGGACTAGCAGGGACGCCGTCAAACTAGCAACGATCACCCGCCATGTGAATTCGTTAACAAGCACTATGAGGAAGGGGGGCCCGAGCAATCCTGCGATTTGAGCTACACGTATGCACTAATGGTAGCCTTAGTGCTCCGGATTCTGAGAGTGGGACTTGACCATATGGAAGGACTCGTCGCCGGTAGCCTTCACAGTGCGCGACGGAAATAGGGCATCTAACGGGTCTTCATGTTTGAGCCTGAATGTCCCAACTCAACGTCTAGCATTATTCCCCAGACGCCGAAATACCCTTTGAGTGGTTGATGTCTGCGTTAATAGTAAAACCATTCTAGCTAGAGTATCGAGCATACGTCGCCTTCGGTTGGATAGGTCGACGGTTCGAGGCGAGAGTAACTACGATCGCGCGGTTTCGAGGAAGTTCATTGGGCA?

Answer: E


Question: I want to clone the fsr gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: C


Answer: A


Answer: B


Question: I want to clone the ymfI gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: A


Answer: E


Answer: E


Answer: B


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: E


Answer: D


Answer: B


Answer: D


Question: I want to clone the flgF gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes KpnI and SalI?

Answer: E


Question: I want to clone the hisG gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: D


Answer: B


Question: I want to clone the fsr gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: D


Answer: A


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: C


Answer: C


Answer: C


Question: I want to clone the hcxB gene from E. coli into the plasmid pUC19. I have the following primers: GAGCTCATGGAAAGTGGTCATCGCTTTGATG, GGATCCCTGTCAGCAGTTAGCCAGCTAA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: E


Answer: C


Question: I want to clone the ubiI gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: E


Answer: B


Answer: E


Answer: D


Question: I want to clone the rtcA gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes KpnI and PstI?

Answer: A


Question: I want to clone the ampC gene from E. coli into the plasmid pUC19. I have the following primers: GAGCTCATGTTCAAAACGACGCTCTGCG, CCCGGGGGCAGATTCTTAACGCTCTACAGTAA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: D


Answer: C


Answer: B


Question: What is the expected amplicon length if I used the primer pair ACTGCGGCAATATCCGTGCACATAAAAA,TGGTCCGGACGGCGCGCCAAGAAA in a PCR from the following template: CCGAGGTGCATTGTCGCAATTTTGTCAGTGACGTTGGTCATTTACAAGCCCGACTGGAAATCTAACATCTGGTGAGGTCGGTGCAGCAATATCAGGCAGCTAATATGACGCGGGACAGTGCTTTCTGGGAGATCTTAACGCCTGTATACAACTGGGTTGTTGACTCCAGACGGACGAAGTGTGCAATATATGATAAAACGACTGGCCTCTACCCACGGTTACCAGACACTCCACCATCGGTTGATCTTTCACGCGCCACGTTTTTTTGCTTATTTGAAACGTAGCCAGACCGCATCCAGACAAGGATCCTATGACGCGGCTATGTCTCAGTAATCGTAAATTAGTTAGACGTTGGCGAACTCTGGATGGCTGAAATCCTGTAGGGAGTGCTAGCGTCCCTCATTTTAACTTTTAGAACCGGGAACGGACCCACCAAGGTTTGCGGATACGTTACTGCGGCAATATCCGTGCACATAAAAATGGGTTAGTCCTTACACAATTGGTTCCTAACCCTACGTGTACGTGTGCCTAAAACAGCAACGCCTGGTTAACTCAGTATCCATTACTCGTAGTCTAAGACCTACATGAGCTGAATCGATTGAGTTAGAGTCCTAGTGGCTGAGAGGAAGCTGGTCTGAACTAAGCAATAGCTAGTAAGTGCAACGAATGTCCAGTGGTGGAAAAAGTCGGCCAATAACTTCCCTAATTCGCATTGAGCTACCTAGTAGTGGCTTATGACACCTTGGTGAGGTAGTCATGCGTAAATATAATTAGTGGGAGCCTTACCCTAATGTAGGGAGTCGAGTTGGGTGGCCAAGAATATTCGCGACATGGATGATACCCGTGGAGTCTAGAGTTCCTCGCAGTCGTGGTGTGGATGCTCACTCCGGCCGGAATGTAATACCCAAAAGGTCCCAGCTCGCAGTAGAGCGTTTTCTTGGCGCGCCGTCCGGACCAGTATCGGGGCGGTTGTACGTGTTTACTAAAGATCGCTTTTTTA?

Answer: D


Question: I want to clone the tktA gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: B


Answer: D


Question: I want to clone the yqcC gene from E. coli into the plasmid pUC19. I have the following primers: AAGCTTATGACCACTCATGACCGCGTTC, GGATCCTTTTGCGGATGATGCGAGCTAA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: B


Question: I want to clone a gene with the sequence ATGAAAAATCCCTATTTCCCTACCGCACTTGGGTTGTATTTTAATTACCTGGTGCATGGTATGGGCGTCCTTTTGATGAGCCTGAATATGGCCTCGCTGGAGACACTTTGGCAGACTAATGCCGCGGGTGTCTCGATAGTTATCTCATCGCTGGGCATTGGTCGATTAAGTGTCTTGCTTTTTGCAGGATTATTATCCGATCGCTTTGGTCGCCGCCCTTTTATCATGCTCGGGATGTGCTGCTATATGGCCTTCTTTTTTGGCATCCTGCAGACCAATAACATCATTATCGCTTATGTTTTTGGCTTTCTGGCGGGAATGGCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATCATTAGCCTGTTAGTGTGGGCTGAACTGTGGTTCGGTTGGTCCTTTATGATTGCTGCAGGCATTATGTTTATTAACGCTCTGTTTTTATACCGTTGTACGTTCCCACCCCATCCGGGTCGTCGCTTACCTGTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGACAATTTGTTGCAGGCATGTCATACACTATGTCGATCAAACTACTCAGTATCTACACCGTGGGTTCGCTGCTTTGTGTATTTATTACCGCTCCACTCATTCGTAATACCGTTCGCCCAACAACATTACTGATGCTGTACACCTTTATCTCATTTATTGCTCTGTTTACCGTCTGCCTGCATCCCACATTTTATGTGGTGATAATATTTGCTTTTGTCATTGGTTTTACCTCTGCTGGAGGTGTTGTGCAAATTGGCCTGACGTTAATGGCTGAACGTTTCCCTTACGCTAAAGGTAAAGCTACAGGGATCTATTACAGTGCGGGCAGTATTGCGACCTTTACTATTCCGTTGATTACGGCTCATCTGTCCCAAAGAAGTATTGCCGATATTATGTGGTTCGATACCGCCATCGCTGCCATCGGTTTTTTACTGGCACTGTTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SphI and SmaI?

Answer: D


Question: I want to clone the ygjH gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes XbaI and XmaI?

Answer: D


Answer: A


Answer: C


Answer: A


Answer: D


Answer: D


Question: What is the expected amplicon length if I used the primer pair TCATAAGTGATCGTAAAGCCACC,TATCGCAACTGAGTCTCTAGAACC in a PCR from the following template: TTGGCAGGCAAAGCCATGTCCTTTAGGGGAAAACGATAGATGATTCGAGAAGCCGCATTGAATCCAAAATGTACACCTTAGCTCGAGGAAAAAATCCACGATTTTGAGACTATAGTTCTCGCCGGCAGTTGACGAATGGCTATACCACGCAAGTTCCATTATGTCTATGACGATCAAGACCAGGCCAAACGCGAATGACTCAATTCATATGAAAGTTAGTGCCTTCGCCGGGCTACTCCTAAACCGCCTGAAATGATTGCTGCAATTACCTTTGGAGAGGGACTCCTCTGTCAATGCGATACCAGAGAAGCCTCCACGTACACCAGGCACCTTCAAGTCAATTCTTAAGCCAGGAAGGTAGCTGCCGCACTCTTACAGCACATCAGAAGGACTTTAAGTACATCGTTTAAATCCCTATCGCCACGATAAGCCCTTTGCCGTTCCACTCCGTTCTGGATAGGTCGAGCGCGCGCTTGTTACGTGTGATACTATCATAAGTGATCGTAAAGCCACCCTTACAACAGTAGACCGTGTTAGGTTAAGCGGGAATACGAGTACAAGGTGAAAGTATTACTTTGACGGTTCTAGAGACTCAGTTGCGATACCGTGGTACGAGCATACTCCCAGTGATTACTCGCGGCCGGACCGTCCGAGTACGGCATTGTTTCACTACCGAATACTACAACGAGTATTTCCTTTTAGGGTGACACTCAGTATTCTGGTCTTAGACTGTGAACAGGTCTCCTTTAAAGATACCTTTAAGATCCAGAGCTAGCGGTTTAGGCTTGAGTGCAACAAGGTAATGGGTAAGGAGAGTCTGTGGGGAAAGAGCAAGGGAAAGATGTCCATACAACTGGATCACAACCGGCTTCTGTTAGAACTAGGCTCGCGAAAATTCTCTGAGGACCGGGGCGCTGCCACGTCACAAAAGGTAGCAATGCGAACGTGCCCGCGGGGGTAGCCAATAGCGGAAACAACGTCTGCTTGCGAAAAACTGACC?

Answer: A


Answer: E


Question: I want to clone the nrfC gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: B


Question: I want to clone the glaH gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: D


Question: I want to clone the ybaL gene from E. coli into the plasmid pUC19. I have the following primers: GGTACCATGCATCACGCCACCCCGCTTATCACCA, GAGCTCACCGGCGGGTGAGGTGGTGACGGGGTAA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: B


Answer: C


Answer: B


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: E


Answer: B


Answer: C


Answer: E


Answer: D


Answer: E


Answer: B


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: E


Question: I want to clone the dhaL gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: E


Answer: A


Question: I want to clone the avtA gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: D


Answer: C


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: D


Question: I want to clone a gene with the sequence ATGAACGTTTTTAATCCCGCGCAGTTTCGCGCCCAGTTTCCCGCACTACAGGATGCGGGCGTCTATCTCGACAGCGCCGCGACCGCGCTTAAACCTGAAGCCGTGGTTGAAGCCACCCAACAGTTTTACAGTCTGAGCGCCGGAAACGTCCATCGCAGCCAGTTTGCCGAAGCCCAACGCCTGACCGCGCGTTATGAAGCTGCACGAGAGAAAGTGGCGCAATTACTGAATGCACCGGATGATAAAACTATCGTCTGGACGCGCGGCACCACTGAATCCATCAACATGGTGGCACAATGCTATGCGCGTCCGCGTCTGCAACCGGGCGATGAGATTATTGTCAGCGTGGCAGAACACCACGCCAACCTCGTCCCCTGGCTGATGGTCGCCCAACAAACTGGAGCCAAAGTGGTGAAATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGCTCAGGGGGCAGTGCATTTCCCCGCGGATGTTCAGCAACTGGATATTGATTTCTATGCTTTTTCAGGTCACAAACTGTATGGCCCGACAGGTATCGGCGTGCTGTATGGTAAATCAGAACTGCTGGAGGCGATGTCGCCCTGGCTGGGCGGCGGCAAAATGGTTCACGAAGTGAGTTTTGACGGCTTCACGACTCAATCTGCGCCGTGGAAACTGGAAGCTGGAACGCCAAATGTCGCTGGTGTCATAGGATTAAGCGCGGCGCTGGAATGGCTGGCAGATTACGATATCAACCAGGCCGAAAGCTGGAGCCGTAGCTTAGCAACGCTGGCGGAAGATGCGCTGGCGAAACGTCCCGGCTTTCGTTCATTCCGCTGCCAGGATTCCAGCCTGCTGGCCTTTGATTTTGCTGGCGTTCATCATAGCGATATGGTGACGCTGCTGGCGGAGTACGGTATTGCCCTGCGGGCCGGGCAGCATTGCGCTCAGCCGCTACTGGCAGAATTAGGCGTAACCGGCACACTGCGCGCCTCTTTTGCGCCATATAATACAAAGAGTGATGTGGATGCGCTGGTGAATGCCGTTGACCGCGCGCTGGAATTATTGGTGGATTAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SstI and XmaI?

Answer: B


Question: I want to clone the yccF gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: E


Question: What is the percent GC of the DNA sequence CTTCGAAGACTTAATCAAAGGGCAGCGCGCGACTCCCCCTCCCGTTAAAGACTCCAGCCGATGTCTCCATCGGATGTTCCGGTACGGTAATTACTGGTACTATGGGAATTAAGGGTTCCTGGCTTGAACGCTCGTCGATGGACAGTGACCCGTATCTCTGTACGAAAGTGTTTATACGGGGCTCGCAGACTGGGCAAGTGATTAGCTCTCCCCATGAGGAACATTAATGTAATCTATAGGGATGAGCAAACTGAGGCGTCATTCGATGTCTTAACTCAGTCGCGGCCGGGTAGCGTTTTAGGCTGCGCGAGGGGATTTTGACGAAACTTGTCCCTCTATTCGAGGCGCGCGACATCTTAATGGCAATCATAGCGGGTCAACCAACGAGCAGCCCGACACCGGGGGACCACACACTCGCTGAACCCTCGAATGTGTAGCCGAAGGAAAGGATGCACCAAAGGAGTACAGGCAGACCGCCTCCTTGGAACGTTTAGGGTTCTGAGTCTTTGCGCCATCTTTTGAACTGCGTTGCACGCCATTGGGAGGGGACGGTTTGTAGCCATTCCGATTCCCTGTGTACATGCTTTTCTTACCCACCTACTGCGCATGGAATGGACGCTGAGTTTCCTGAATAGTGTCAGCCATACTGAACTTCGTAGCCCGCGCCCATGACCTCCAACCCGC? Round to the nearest integer.

Answer: B


Question: I want to clone the cca gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes KpnI and SalI?

Answer: A


Answer: C


Question: What is the percent GC of the DNA sequence TCTAGTACCTGCGTCTACCGTAGTGGTATGGGCACTACAGTTAAAATTCCGCACCTACATCTTGCAGACTATGCCCGGATGATTCTGCGCGCCACCCCCCGCCATTGCGTTCTACGTAGTCCTGCCTTCGCAGAGTAACGCTGACCATAGCTCGTGCCAGATGTATTTTCGAGTCATACTCTGGTCATTCCCGTAGTTACATACTAGGGGGTAGCAATCCTTGGAGTTCATGCTAACAGCCCCTTGGCAGGGTTCCCCCCTAACTCTCTATTAGATAATCGTTGATTGGTGCGCTCCATTTATCACCTA? Round to the nearest integer.

Answer: A


Answer: D


Question: I want to clone the hyuA gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SacI and XmaI?

Answer: A


Answer: E


Question: What is the expected amplicon length if I used the primer pair ACAAAGGCGCAACCGGTCTTGTATT,GTGTCCCTAATGAGAAATCCTCCT in a PCR from the following template: CGTGATATGAAGGCGGGGAGGCCGGCGCCAGTCTCTGCTTCGTCGCGCTTGTAAGGTTAGTATGAGGTCGAAACTTGACTTGAGAAAATCAGCGCCGCTCCTTTGGAGGACGATACGTTCCTCGTGCGCGTTGGATAGACCAGACGCTCTATTAACTAGGGCCACTTAGCAATAGATGAGCTCTATGATTCGAATTTACAAAGGCGCAACCGGTCTTGTATTTATCGTAACGAGAGTGATTTTCTGGCGCCGACTGACATACGATTATAAATTCTTCGGCTAACACCTAAGCAGACTTATCCCTGGTCGCAAAGACGTCCGTTAGCAATACCATCCCGGAGAACGCGACGTCACAGACAGCGATGGGTCAGATAAGGTTCAACGCTGAGCTTATATTCGGATCTTTGAGGCGCTGAGCTACGTCATTGAGCCGATCGGGTGGAGGGTGAGGCAAAATGTATTTCATTTAAGTATTAAACACCCACCCAGCCACGGTTACTCCCACAGTTATCTTCAATAACTGCTGAAGTGCTTAAGGTTATTTCGTAAATCTAGATTTCTGGTCGCGGACCACCATACGTCGAATTTGAGGTTCGTGTATGACCATAGACCTGCGACACTTAAAAGCGTAAGACCGGTAGTTGCTCCTACTTGGAAGCGGCTATCCGAGACCAGGAGGATTTCTCATTAGGGACACCTGAACCCGGTCGATATTACAGTTAGATCCAATGCTATCCAGTTTGTACGGGCTTTGAGCAAGCCTAGTCCCACAGAATGACACAGAATCCACGCTCTACACGTTCACGCACGACGACCCTCGTGCGTCGAGAACCCTCGGTACAGGATTACTGTTGGAAATCTAACTCGTACATGGATCCGTACGGGCCGATTAACCACTGTTGTAGAGTGACACGGGTTGAGGCAGGGGCAGAAACTAGGAGATGACAAGGATAGGACAGGCGAGTGGTGCCACGAGAGCCGGCCTCCGTGTCCTGTACAA?

Answer: B


Question: I want to clone a gene with the sequence ATGAGTGAACCCCTGTTAATTGCCCGCACGCCGGACACAGAACTGTTTTTACTGCCGGGAATGGCTAACCGTCATGGGCTGATTACCGGCGCAACGGGGACGGGTAAAACCGTTACGCTGCAAAAACTGGCGGAGTCATTGTCGGAAATCGGCGTGCCGGTGTTTATGGCTGATGTGAAAGGCGATCTGACCGGCGTCGCGCAGGCAGGAACGGTGTCGGAAAAACTGCTCGCAAGGCTTAAAAATATCGGCGTCAATGACTGGCAACCGCATGCCAATCCGGTGGTGGTGTGGGATATCTTTGGCGAGAAAGGCCATCCGGTGCGGGCGACGGTTTCGGATCTGGGGCCGCTGTTGCTGGCACGACTGTTGAATCTCAACGATGTGCAATCTGGCGTGCTGAATATCATCTTCCGCATTGCTGACGATCAGGGGCTGTTACTGCTCGACTTTAAAGATCTGCGGGCAATTACCCAGTACATCGGCGATAACGCCAAATCCTTCCAGAATCAGTACGGTAATATCAGTAGCGCATCGGTTGGTGCCATCCAGCGCGGGCTGTTGTCGCTGGAACAGCAAGGCGCAGCACACTTCTTTGGTGAGCCGATGCTGGATATCAAAGACTGGATGCGCACCGATGCCAACGGTAAAGGCGTTATCAATATCCTCAGCGCCGAGAAACTTTATCAGATGCCGAAACTGTACGCCGCCAGCCTGCTGTGGATGCTTTCAGAGTTGTATGAACAATTGCCGGAAGCGGGCGATCTGGAGAAACCAAAACTGGTGTTTTTCTTCGACGAGGCACATCTGCTGTTTAACGATGCCCCGCAGGTACTGCTGGATAAGATTGAGCAGGTGATAAGGCTTATTCGCTCAAAAGGCGTGGGCGTCTGGTTCGTTTCGCAAAACCCGTCTGATATTCCGGATAATGTGCTCGGGCAGCTCGGTAATCGCGTTCAACATGCTTTGCGGGCTTTTACGCCAAAAGATCAGAAAGCAGTGAAGGCAGCGGCGCAAACCATGCGGGCCAATCCGGCGTTTGATACCGAAAAGGCGATTCAGGAACTGGGCACCGGCGAGGCGTTGATCTCTTTTCTGGATGCGAAAGGAAGCCCTTCTGTGGTGGAGCGTGCGATGGTGATCGCGCCTTGTTCGCGGATGGGGCCGGTGACGGAAGATGAGCGTAATGGCTTGATTAATCACTCTCCGGTGTATGGCAAATATGAGGATGAGGTGGACCGGGAATCCGCCTATGAGATGTTGCAAAAAGGCTTTCAGGCCAGTACCGAGCAGCAAAATAATCCTCCCGCGAAAGGGAAAGAGGTAGCGGTGGATGACGGCATTCTTGGTGGATTGAAGGATATTTTGTTCGGCACTACCGGACCACGCGGCGGGAAGAAAGATGGTGTGGTGCAAACAATGGCCAAAAGCGCCGCTCGCCAAGTGACGAATCAGATTGTACGCGGGATGTTGGGGAGTTTGCTGGGGGGGAGAAGAAGGTAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes HindIII and XbaI?

Answer: E


Answer: A


Question: What is the expected amplicon length if I used the primer pair TAGTACACGCTACCTTGCCTAGAGCTCC,CGGATGGTGTTTCAGGAGCTCTC in a PCR from the following template: GAGAGAGACAGTTGATGTTACTAAACTGGCACGTGACGCGCAGAGAGGGGAAGTTCATGCTACGCGTCGCGTAGCTCTTTTGAGCGATTTAGTACACGCTACCTTGCCTAGAGCTCCCATATAGGGTGGAGCTCTCACCTCAGCCGCACGGGCAGCCGAGTTTGAAGTCTGGTTCACACAGCATGCGACTACTCGGTCTAAATTCATAGCGCAAGTATTGGGGGAACACCAGAAGAACACAGGTTCGCGCACGGCTATCACACGATCCTGTTCTAACCGTCGTTCTTGGCTATTGAATCCATTACCTAGGCTTATGCGGCGCGATCGTATTTTTGAGTGTAGGCCCGGCCAAATCGTGAGAGCGTGTCCGGGCTCTACGTGCGGGACGCGCTCATGGAGCGTCTGCCCTCCTCGCAGCGCGCAGCGACTCGATACCCACAAATTACGAATGGATGGCTCTAACTTGTCTTGCATTAAATAGAAACCGTGCGTCGATCTGCCATTGACTCTGCACGGTTTAGACGCTAAGAAACTGTGTTGCTATGCAAGCCTAAAATAGCTATGAGCGCACGCTCAAGAAAAGCCTACTCTATGAGAGTCTACCCTATACTTCTGCATCCACGACCTTGGAAGCCCTGCCCTCAGCACAAGCTTCTGCAACAGAGCCATGGCCTATTCACTAACATTCTGTAGGTCGTGTTTCAAAGGATCGTGTCCTTTGTCATACACGGTAATACTCCTACTGAACGGACTTATCTATGACCGAACCGCAAGCATTGACTATCAGACTATATGGGATTATAAGCTAGGACCCCGAAGAAATTATACCCTTGATAGCAAAAATACATCACAGGTAGAAGTTCGCGGCCCGAGGCGGAAAGGGCTATTGCCAATACGCATAGAGGAGAGCCCACCCGGTATCAGAGAGCTCCTGAAACACCATCCGCAATTCAAGCCGGTAAAAGCAACTAATGTGCAAGTAGCCTACACAGTTTTCCAG?

Answer: B


Question: I want to clone the alsR gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: D


Answer: B


Question: I want to clone the yfaZ gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: A


Question: What is the percent GC of the DNA sequence GATAATATCGTATTAACGTTGAAGCGCAGCCACTAGCGCGTTCGGTAAACGTAGGTGCCATACAAAATGACCGGCGTGATACTCACCTGTCCCCCTAAGCATCCTGGGCTTTCCTGGTTAATTGAATGGTTAACCGCTATGATCGGTCGGCAAACTTGTGCGGAGCCCGTAAACCGGTGTCATTCTTGACTCGTGCGCTACTAAACTTGTTGTCCGAATTCCTTGACTCAGTCCGATCCGAATGAAACATTCTTCGCGGGAACTCGTACACTTATACGTCCTATTGCGCAGACAAATAATGTAGCAACCCGATGGGAGGAGAGAGAATTCATTCCAGGTAGTATCCCAAAGTTAGAGGCCGACTAGCATTTCACGTTGCTAGGGTCTATATCCCTACATCAAACTAGCCAGAGTTACGTTCGATTGCTCAACGTTGGACTGTCGGCAACCTAAATTATGTGCGTTTCGTGGTGATTTGCAAAAACCTGCGTAGAAAGTCGTTACAGATACTGTGCGCATCTAACTAACTGTTATGCTCCTGGGGATAGTCATGCCTTGCCTACTTCTATCTCGAATCTCCTCGGTTAAGATTTTCAGCGATCCGAAGCTCGGCCAATCTTAATGCCA? Round to the nearest integer.

Answer: E


Question: I want to clone the mmuP gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: A


Answer: B


Question: What is the expected amplicon length if I used the primer pair TCAAGCTCAACCACAGGACCC,GATGGCAGCATCTTGATCTACT in a PCR from the following template: ATGGCCAACCCTTTAAGGCACTCCGGATAGAGGTCGCCTGATCAGTCCTGTTCGAGGATAAAAGTTAATCCCGGGAGGCAAAGGCAAGAGTGACCCGTTTAATCGCCCCTTATTAGGTGACGTACAACCGTTGAGATCGCGCAAACAGATCACCTATACTACCCCCGTCGTGCTATCACTTCAAGCTCAACCACAGGACCCGAATACGGAGTCCGGGTCACGGACCCCAAATATTAGGTGCCCCTGGGGTGAACCCCTCATATTACCAACTAAATCCTCCACTATATCAGCAGACAGAAGGTGAACTACCGTTCATGTGCATCCGGGAATCTATGTAACTGAAGAGTTCGAAACGAAACCCCACTGGTCCGCAGATGGAGACGCAGGGGCTTTGGTTGACACTTTAGGCCACACTAGTTTCCCCAGACGCGTGGGTTCCGGCTGAATCGTAACTTGTATCACTGGCAACGCCGCTTTCTCCTTCGCCAAGCAACGCTCCAGGAATAGGGACGACAAGCTGCCACCGTCTGAAGTCCGAGTCAATTCACTAAGAGTATGCACTGGTCTGTGACGGAAGCTGACATTTATATGCCGACACGACCGCCAGTCCGCTCGCCTGAATTGCTAATTTGAGCTTCACGACGAGGCATGAGAGGTAATTTATTCCCCAACCTAAATCCATGCCCCCAATTGAAATGCCCATAGCCGGCATTTACCTGCAAATCGAGGAAGGCAGTTAGCGGGTATTTGTTCCAGTAGATCAAGATGCTGCCATCTCACTTGGTCTATACAGATGCGCGTTTGCCGCTGCACCCAGTCTCATCATCGTAGTGAAGTTCGGCGCCTTCGGGAATATAAGTTAGGCACGACGGCTAGCGGCTTGAAGAGATGGACTGTAGACCAGAAACATGTTATATCTTTTTTCAACTGCGCACTCTTCGGGCAATAAAGCGCAGTAGAGTGTATGTGCTTTTATTTTCTGTGGTGTGGACAGGGTGTT?

Answer: E


Question: I want to clone the ydhW gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SalI and XbaI?

Answer: A


Question: I want to clone the paaG gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes HindIII and BamHI?

Answer: C


Answer: E


Question: What is the percent GC of the DNA sequence CGGGTAATGGATCGGCTTACGTGTACCCCCATGGCGCACATCTCCTCTTGGATTAAGTAATCCTAACGTTCGATCCAAGTGTAATCTGTACTTTCATTTCCGGGACGACACTTTCGAATCATGTACGCACCCCACGGAATGCGACAGTGCTCACAGACCCTCCTAAAAACTCTGTACATCTGGTGGATATATATGTCTTAACGCCTAAAACGTTAGTCAGCTCTTATACTGCAGGTATTGGCCCAGAGAGACTAAAGCGCGCCTGAATGCGTCGACTGAGTACCCAGTCGCGTTCCTTTTATATTCAGCCCAATGTATACGTAAACTTCCGACTAGATAGCCGGACCGGGAACCACCCGCTTCTAATCGAGGTTACTTACACGACGTTCACCCCAAAGCGAGGGGAGTAAGGAATCCTATTTGGTAAGCGTACCTTTGGATCTGGCGGCCTGTAGAACTACTGAAATAAGGGCGTTGCCGGTGCGATGAGGGCTTGTCGTCATTTTCTTAGCCAGGCATTCCACGGCCACGCCGATCATTTTAGAGTTTTGTCACCCACCCATCGCTCAACGGAGCGTATCTACGTCAACCACCGCGACGTGGAGCACGGGGTAGTATCTGCCAATCAGCGCGGTAATAATGACTAACATAGGAATTATCGGAGAGCGAGGACCTACAATCGATCATCAGTGATCGTTTCATCTTTCAGCAGCCACTTGGTGGAAGCGATATAAAGAACTA? Round to the nearest integer.

Answer: B


Question: I want to clone the macA gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SalI and HindIII?

Answer: E


Answer: C


Answer: A


Question: I want to clone the agp gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: D


Answer: A


Question: I want to clone the feoA gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: C


Question: What is the expected amplicon length if I used the primer pair CAGGCCGCCAAGGGCCCATAG,AGTACTGCGTAATAGCACAGCCCGT in a PCR from the following template: AACAGGCCGCCAAGGGCCCATAGGACCGTTGCCTCCACAAACCGTAGGTAAGGGTTAATGGGCTATGTATCGTCTGCATACGCTACGTTTAGTGGTGAAATTTGCTGCTTCGTCCGAGCGCGGTCGAATCAGCCTCGGGTTGATCTATTATTGAACGGGTATGTTGCGGCCAGTACCAGTAGCTGGCAGGTTACGAGCATGGGACAGAGAGGTTCACCTAAAAGAGCGGCGATTTACTTTAGACGCTCTTTAAGGCGGCGGTCTGTACCCTCTACTCGCTAGCACTGGGTTCAGACAGTTGCAGGGCTCTTGAGTATCCTTCACACTTCCTATCGCTGAGCGATCCGCGTTAATTATAGCTTATCCGATGCCGCGGTTCGGGCCAGACAATGACAACTCTGCTTTGATGCCCACTGGTTGCGTTACTGCGTATGCTATATCAGAAATCATGGTGGAGACCCGATGCTGTTATATAGTATCATCGCCGATATCAGCGTCAACCTTCTGAAAAAGTGAGGGGTACGCTGGACAGCCACCCCTCGAGCACGACGACTGGTCGGCCTGCGTATTAAGACGTTGCCATTGGGTAATGACAAGACATCTCGCGTATATTGAGCCAGCACTATAATATTATCCGGATATACGTCCAGCCGTGAAACTAAGCAAAGGGGCATGATTGGAAAAAGGCACGTTGATCCACGGGCTGTGCTATTACGCAGTACTTTGAGGGACTTGAGTGTTTCATATGTCTTCATAAGACTTGAGACTAGGAACTACGGATTCAGCGATCCTTCTCGCGCTAGCCCAATTCACAAGACCAGAATCGGTCCAGGGCCAAGTTGGGTTAGGTCACAAACGGTAGGTTGCCCCTTACAATCGTTAACACTGGCTATTCGCCTTTGGGCTAGTTATCATTACATCAATATCAGACAGACTGGTTGTGGAAAAGCGTCCAAATTAGCTGAATCCCGATACGTATTAGACGTGTAGGACCACTAAA?

Answer: A


Answer: A


Answer: E


Question: I want to clone the yefM gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: A


Question: I want to clone the blc gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes BamHI and SmaI?

Answer: A


Answer: B


Answer: C


Answer: C


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: B


Answer: D


Answer: B


Answer: C


Answer: A


Answer: C


Answer: B


Question: I want to clone the cspF gene from E. coli into the plasmid pUC19. I have the following primers: GCATGCTTGTCCCGTAAAATGACAGGAATTGTC, GTCGACAGCTGCCAATGTTTACCTTTCATGA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: A


Answer: D


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: C


Answer: B


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: B


Answer: A


Question: I want to clone the yphF gene from E. coli into the plasmid pUC19. I have the following primers: GGTACCATGCCTACAAAAATGAGAACAACCCGT, CTGCAGGCAACGCACGTTGATGGTCTGCCCTAA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: A


Answer: B


Answer: E


Answer: C


Answer: E


Question: I want to clone the gspE gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SalI and BamHI?

Answer: A


Answer: B


Question: What is the percent GC of the DNA sequence CTTTGTTCTACAATTCGGTCCCAGAGCTTTTTAGTCAAAGTAAGCTTGTGTTTCCATTATACTAGGGGTTAAATTCCACGTGAGACTAGGATTTCATTACGCACGTGTCATCTTGTTGGGCGTGTAAGAAGGACTTAGTAGTTCGTGTAAAATAAGTCAGCCAATGTCTTGTGCCACCACGTTACCTGTCGTTGCGTGCCATGGTCGTCTCTTATCCAGAACTGAACTGTCTATGGAGCACTACCATAACTGACTGGTCTCGTTGTCCTATAGTTGACCAAGTTCGTTTTCAAAAACGCTATACTGGTCTCCTTCCTTCTTGTAGTAATATCTATGTTGAAAAGGGTCAACATCGAATGCGGGCAGACGAAGTGGAAAAGCAGTTTAGGCTAGGATTGGTTAACAACGGGCAGGAAGTTTGGGGTTGTTCCCGTCCAGACTACTACGATCTCGTCACAATGACGATGTCAGGTCGTCACTCTTCCTTTTGTTCGCTAGGCTTAATCTTGCTAA? Round to the nearest integer.

Answer: E


Answer: C


Answer: E


Question: I want to clone the ppnN gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: E


Answer: B


Answer: C


Answer: B


Question: I want to clone a gene with the sequence ATGACTTACATGAAATTAACGGCGGCTAAACGCATTGTTTTTTTTATATATTTATTTGTAATCCAGTTTTGGAAAAACGCCAGTTTTCAAACGAAAGTCAGTTAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SalI and BamHI?

Answer: B


Answer: D


Question: I want to clone the alkA gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes BamHI and SstI?

Answer: B


Question: I want to clone the ddpD gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: C


Answer: E


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: C


Answer: E


Answer: E


Answer: B


Answer: A


Question: I want to clone a gene with the sequence ATGACACAACAAGGGGATGCTGTAGCCGGGGAGCTGGCGACAGAAAAAGTGGGCATTAAAGGGTATCTGGCCTTTTTTCTCACCATAATCTTTTTTTCCGGTGTTTTTTCCGGAACAGACAGCTGGTGGCGCGTTTTTGATTTTTCCGTTCTGAACGGATCGTTTGGGCAACTGCCTGGTGCGAACGGAGCCACCACCTCATTTCGCGGCGCGGGTGGCGCGGGGGCTAAAGATGGCTTTCTCTTTGCGCTGGAGCTGGCACCGTCAGTCATTCTCTCTCTGGGGATTATTTCAATTACCGATGGTCTTGGTGGATTACGCGCCGCGCAGCAGTTAATGACACCTGTGCTAAAACCGTTGTTGGGCATTCCAGGGATCTGCTCGCTGGCGCTGATCGCTAACCTGCAAAACACCGATGCCGCCGCCGGTATGACCAAAGAGCTGGCGCAGGAAGGTGAAATTACCGAACGCGATAAAGTGATTTTCGCCGCATATCAAACCAGCGGTAGCGCCATCATCACCAACTACTTCTCTTCCGGCGTCGCGGTGTTTGCCTTTCTGGGCACGTCCGTAATCGTACCTTTAGCCGTCATCCTGGTGTTTAAATTTGTCGGAGCCAACATTTTGCGCGTCTGGCTTAACTTCGAAGAACGTCGCAACCCAACGCAAGGAGCGCAAGCATGA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SphI and SmaI?

Answer: C


Answer: D


Answer: E


Question: I want to clone a gene with the sequence ATGGCTGAGATGAAAAACCTGAAAATTGAGGTGGTGCGCTATAACCCGGAAGTCGATACCGCACCGCATAGCGCATTCTATGAAGTGCCTTATGACGCAACTACCTCATTACTGGATGCGCTGGGCTACATCAAAGACAACCTGGCACCGGACCTGAGCTACCGCTGGTCCTGCCGTATGGCGATTTGTGGTTCCTGCGGCATGATGGTTAACAACGTGCCAAAACTGGCATGTAAAACCTTCCTGCGTGATTACACCGACGGTATGAAGGTTGAAGCGTTAGCTAACTTCCCGATTGAACGCGATCTGGTGGTCGATATGACCCACTTCATCGAAAGTCTGGAAGCGATCAAACCGTACATCATCGGCAACTCCCGCACCGCGGATCAGGGTACTAACATCCAGACCCCGGCGCAGATGGCGAAGTATCACCAGTTCTCCGGTTGCATCAACTGTGGTTTGTGCTACGCCGCGTGCCCGCAGTTTGGCCTGAACCCAGAGTTCATCGGTCCGGCTGCCATTACGCTGGCGCATCGTTATAACGAAGATAGCCGCGACCACGGTAAGAAGGAGCGTATGGCGCAGTTGAACAGCCAGAACGGCGTATGGAGCTGTACTTTCGTGGGCTACTGCTCCGAAGTCTGCCCGAAACACGTCGATCCGGCTGCGGCCATTCAGCAGGGCAAAGTAGAAAGTTCGAAAGACTTTCTTATCGCGACCCTGAAACCACGCTAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes EcoRI and SacI?

Answer: D


Answer: B


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: A


Question: What is the expected amplicon length if I used the primer pair ATTTTGACGGCGCTATTCGAA,TATTAGGCCCCGGGCAGGGGTGGCGCA in a PCR from the following template: TATTAGGATTTCGCGGTTCAGATTTTGGGTTGCGAAGGTGGGGAGACAGCATCCTAGTCCTATAAGCATTACATGGATGGGGATACAAGAAAAGACGCGGCTACTCCGCTTAGGGAGAAACTTGGTGCAATTCCTGCAGCGAACTACCGCTAGCGCTGTGGAAGATTTTAAATAAGCGGGCTGAGTAGTCGCCTCGTTTGCGCATAACATGCAGGTACGTCAGAAGGGAGTCCCCCTTGGGGGGATTAGGCCTGGAACTACTCGTCATATACCCGTTCTTAATCAAGTTGCAGACGTGTTTACCTTCATATTTGCAAATTTTGACGGCGCTATTCGAATATAAGATTTGATCCCCATCGGCTCCGCACCCCTCCTCTTGACATCCCCATGGCACTCGTAATAGCCACTTCCCTTTTAGCTCGCGACGGGTACACTTGTAGATTCAAGTTAGACCGGACGTGAGATCTCGGCAGTGGTTACGTTACATGGGATTGGATAAGATATCTGCCCGGAGTTCAAACTTCTCCCAACGGCCGCGGGCCCCCGTCGGGCATCATACGGCCTGTCCCCCGGGTATCCGAGCCTATTGATTGGAATACCGGTTGACTACTAAAACATCCCCGAAGTCCGTAGCTCCCAATGGTACGCCATGGTGTTTGCGCCACCCCTGCCCGGGGCCTAATACAACTAGTTGCCACTCATCCGTATGTGAGCCAACATATATAGTACGACGAACTATCAGCTAGACACAAAATTCCGGATCTTTTCATTCACCGGATTAAATATGGAGGCCCGTGCTCTCATAGACGACGCGGGGGCGCGATCTTCAGTCCCTATATGTTACGATTCTCCATTCGGAGGCGGGGGGTATCGTATCATCCCACTGACTAGGTCCGTCCAAATCCATCCACGTTTAACTTGGCGGTTGAGTGGAGAGAGGTGTTTATATAATATTGCTGCCGTTATGAGCCATAGGCTAGAATAGAAACCTCCACAGGAC?

Answer: D


Answer: C


Answer: E


Answer: B


Question: I want to clone the tsf gene from E. coli into the plasmid pUC19. I have the following primers: GGATCCATGGCTGAAATTACCGCATCCCTG, GCATGCTGCTGCGATGTCCAAGCAGTCTTAA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: B


Answer: D


Answer: D


Answer: B


Question: I want to clone the sufE gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: E


Question: What is the percent GC of the DNA sequence TGTGGTGGTGACAGAGGTAATTTCGGATGTATTCTCGGAAAAAGTGGCGAGGAATGTTGCATGAGCATTACCACACCAATAGATTGTTGCTTCCCTCATATCTTATAGAACCGAGTATATTATGCCAAGGAATTGCGCATCCTACCGGAGGGAGTCTACGGTGGACAACTAGTCAATTGGTATGTAGTAGGTCCCAGACCTGTTGGTACCTGCCATGGACCTCATACCTGGTGTATTAGGGACGGGATAGGTAGTAAACCCATCCGGCAAGATATCTCTGACCCACTGAGGAGCCAGCCTTGCATCCAGCGGCATGCTCGTGGTACGGGTTGCAGT? Round to the nearest integer.

Answer: C


Answer: A


Answer: C


Question: I want to clone the atpB gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with HindII. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: A


Question: I want to clone a gene with the sequence ATGCGCAGTAAGTATATCGTCATTGAGGGGCTGGAAGGCGCAGGCAAAACTACCGCGCGTAATGTGGTGGTTGAGACGCTCGAGCAACTGGGTATCCGCGACATGGTTTTCACTCGGGAACCTGGCGGTACGCAACTTGCCGAAAAGTTAAGAAGCCTGGTGCTGGATATCAAATCGGTAGGCGATGAAGTCATTACCGATAAAGCCGAAGTTCTGATGTTTTATGCCGCGCGCGTTCAACTGGTAGAAACGGTCATCAAACCAGCTCTGGCTAACGGCACCTGGGTGATTGGCGATCGCCACGATCTCTCCACTCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTGGCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGATGTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAGCAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAAGATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATCCGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes HindIII and BamHI?

Answer: B


Question: I want to clone the ppiA gene from E. coli into the plasmid pUC19 using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes PstI and SacI?

Answer: B


Answer: D


Question: I want to clone a gene with the sequence ATGAGCAATCTGAATCCATGCATGACGTGTGGTGCCTGTTGTGCATTTTTCCGCGTCTCTTTTTACTGGGCCGAAGCCGACGATGCTGGCGGTACTATTCCCGCCAGGCTCACTGAACAAATATCCCCTTTTCACCGATGCATGAGCGGTACCAATCAGAAAAACCCCCGATGTATTGCCCTTGCAGGAACCCCGGGCAAAAATGCCTGCTGCACGATATATAAAAATCGATCGTCCACATGCAGAGAATTCGCCATGTCTGGTGAGAACGGAGTCGTCAATGAGGCTTGCAATCGTGCAAGGGCTAAATACGGGCTGACACCGTTATAA into the pUC19 plasmid using restriction-ligation cloning. Which of the following primer pairs should I use to do the cloning with the enzymes SphI and SacI?

Answer: A


Question: How many open reading frames that encode proteins greater than 18 AAs in length are in the DNA sequence GATCGGCCCTCATTGTTGCCCTTATCGATGGCCGACATGATAAAACCCCATGCTTGACGTAGACTCCTCGTTGTATTAAAACTTTACCAGCTCGGATTACGGTTGCGGTGAATGCTGGCACGAACGATGCTGACTAAAGTTATGCCGTGTCCCGTAGACGAAAAGGGCCCAACATCATATCGTCGAGGCGTTAGCGAGCGACCCTTTTGAGGAGTTCATTTAGTTCGTTACCCGCAGGTAGAGTTCTGGAGTCACCGCTCGATTGGAAGTAGTTAAGAACAGTACCAGGCGTGCGCGAAGCAGTAGGGTCACCGATAGAAATGTGCTTCAGCCGAACTCGGGAACAAGTCCCTGTATTCACGCCTTGTTCGAGATGAAACATTTACGCCGCGCCAAAAAACCATTGTAAGCCTGCTTCGACGCCTCTTTCGTACCTATTACGCAGGGTCGTCTATCAGCAGACGCATGATACGTGCACGGTACGGAACGACTTTTATGGGCACAATTGCGAGGATTACTTGGGAAACTATGCAAGGGCTGTACCGAGCAGCAGCTTAGTTCCCCTTG?

Answer: C


Answer: B


Question: I want to clone the yfcS gene from E. coli into the plasmid pUC19. I have the following primers: GGATCCATGTCTGATTTACTTTGCTCTGCCA, CCCGGGAGCAGAGCCGCAAAGGCTGA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: A


Answer: B


Question: I want to clone the acs gene from E. coli into the plasmid pUC19. I have the following primers: CTGCAGATGAGCCAAATTCACAAACACACCATTC, GGTACCCAGGCTATCGCGATGCCATCGTAA. Which enzymes should I use to digest the PCR product and plasmid for this cloning?

Answer: D


Answer: C


Answer: C


Question: I want to clone the yfgJ gene from E. coli into the plasmid pUC19. I'm going to linearize the plasmid with SmaI. Which primer pair can I use to amplify the gene for Gibson assembly into the linearized vector?

Answer: D


Answer: A


Question: Which of the following RNA sequences contains an ORF that is most likely to have high translation efficiency in a human cell?

Answer: A


Answer: A