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VenusMutHub / single_mutant /activity /MT038422_1_kcat.csv
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2442bc0
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4.06 kB
mutant,mutated_sequence,fitness_score
K1K,KKHPFSYDDYKQVDVGKDGMVATAHPLASQIGADVLKKGGNAIDAAVAIQFALNVTEPMMSGIGGGGFMMVYDAKTKDTTIIDSRERAPAGATPDMFLDENGKAIPFSERVTKGTAVGVPGTLKGLEKALDKWGTRSMKQLITPSIKLASKGFPIDSVLADAISDYKDKLSHTAAKDVFLPDGEPLKEGDTLIQKDLAKTFTAIKYKGTKAFYDGAFSKKLAETVQEFGGSMTEKDIKNFNVTIDEPIWGDYQGYHIATAPPPSSGGVFLLQMLNLLDDFKLSQYDIRSWQKYQLLAETMHLAYADRAAFAGDPEFVNVPLKGLLNPDYINARRQLINIDKVNKKPKAGDPWAYQEGSANYKQVEQPTDKQEGQTTHFTVTDRFGNVVSYTTTIEQLFGSGIMVPGYGVVLNNELTDFDAVPGGANEVQPNKRPLSSMTPTILFKNNEPVLTVGSPGGATIISSVLQTILNKVEYGMDLKAAVEEPRIYTNSMTSYRYEKGVPEEARTKLNEMGHKFGSSPVDIGNVQSILIDRENGTFTGVADSSRNGAAIGVNLKKCEK,69.96
R136C,KKHPFSYDDYKQVDVGKDGMVATAHPLASQIGADVLKKGGNAIDAAVAIQFALNVTEPMMSGIGGGGFMMVYDAKTKDTTIIDSRERAPAGATPDMFLDENGKAIPFSERVTKGTAVGVPGTLKGLEKALDKWGTCSMKQLITPSIKLASKGFPIDSVLADAISDYKDKLSHTAAKDVFLPDGEPLKEGDTLIQKDLAKTFTAIKYKGTKAFYDGAFSKKLAETVQEFGGSMTEKDIKNFNVTIDEPIWGDYQGYHIATAPPPSSGGVFLLQMLNLLDDFKLSQYDIRSWQKYQLLAETMHLAYADRAAFAGDPEFVNVPLKGLLNPDYINARRQLINIDKVNKKPKAGDPWAYQEGSANYKQVEQPTDKQEGQTTHFTVTDRFGNVVSYTTTIEQLFGSGIMVPGYGVVLNNELTDFDAVPGGANEVQPNKRPLSSMTPTILFKNNEPVLTVGSPGGATIISSVLQTILNKVEYGMDLKAAVEEPRIYTNSMTSYRYEKGVPEEARTKLNEMGHKFGSSPVDIGNVQSILIDRENGTFTGVADSSRNGAAIGVNLKKCEK,177.48
K500E,KKHPFSYDDYKQVDVGKDGMVATAHPLASQIGADVLKKGGNAIDAAVAIQFALNVTEPMMSGIGGGGFMMVYDAKTKDTTIIDSRERAPAGATPDMFLDENGKAIPFSERVTKGTAVGVPGTLKGLEKALDKWGTRSMKQLITPSIKLASKGFPIDSVLADAISDYKDKLSHTAAKDVFLPDGEPLKEGDTLIQKDLAKTFTAIKYKGTKAFYDGAFSKKLAETVQEFGGSMTEKDIKNFNVTIDEPIWGDYQGYHIATAPPPSSGGVFLLQMLNLLDDFKLSQYDIRSWQKYQLLAETMHLAYADRAAFAGDPEFVNVPLKGLLNPDYINARRQLINIDKVNKKPKAGDPWAYQEGSANYKQVEQPTDKQEGQTTHFTVTDRFGNVVSYTTTIEQLFGSGIMVPGYGVVLNNELTDFDAVPGGANEVQPNKRPLSSMTPTILFKNNEPVLTVGSPGGATIISSVLQTILNKVEYGMDLKAAVEEPRIYTNSMTSYRYEEGVPEEARTKLNEMGHKFGSSPVDIGNVQSILIDRENGTFTGVADSSRNGAAIGVNLKKCEK,231.42
V158A,KKHPFSYDDYKQVDVGKDGMVATAHPLASQIGADVLKKGGNAIDAAVAIQFALNVTEPMMSGIGGGGFMMVYDAKTKDTTIIDSRERAPAGATPDMFLDENGKAIPFSERVTKGTAVGVPGTLKGLEKALDKWGTRSMKQLITPSIKLASKGFPIDSALADAISDYKDKLSHTAAKDVFLPDGEPLKEGDTLIQKDLAKTFTAIKYKGTKAFYDGAFSKKLAETVQEFGGSMTEKDIKNFNVTIDEPIWGDYQGYHIATAPPPSSGGVFLLQMLNLLDDFKLSQYDIRSWQKYQLLAETMHLAYADRAAFAGDPEFVNVPLKGLLNPDYINARRQLINIDKVNKKPKAGDPWAYQEGSANYKQVEQPTDKQEGQTTHFTVTDRFGNVVSYTTTIEQLFGSGIMVPGYGVVLNNELTDFDAVPGGANEVQPNKRPLSSMTPTILFKNNEPVLTVGSPGGATIISSVLQTILNKVEYGMDLKAAVEEPRIYTNSMTSYRYEKGVPEEARTKLNEMGHKFGSSPVDIGNVQSILIDRENGTFTGVADSSRNGAAIGVNLKKCEK,119.6
V319A,KKHPFSYDDYKQVDVGKDGMVATAHPLASQIGADVLKKGGNAIDAAVAIQFALNVTEPMMSGIGGGGFMMVYDAKTKDTTIIDSRERAPAGATPDMFLDENGKAIPFSERVTKGTAVGVPGTLKGLEKALDKWGTRSMKQLITPSIKLASKGFPIDSVLADAISDYKDKLSHTAAKDVFLPDGEPLKEGDTLIQKDLAKTFTAIKYKGTKAFYDGAFSKKLAETVQEFGGSMTEKDIKNFNVTIDEPIWGDYQGYHIATAPPPSSGGVFLLQMLNLLDDFKLSQYDIRSWQKYQLLAETMHLAYADRAAFAGDPEFVNAPLKGLLNPDYINARRQLINIDKVNKKPKAGDPWAYQEGSANYKQVEQPTDKQEGQTTHFTVTDRFGNVVSYTTTIEQLFGSGIMVPGYGVVLNNELTDFDAVPGGANEVQPNKRPLSSMTPTILFKNNEPVLTVGSPGGATIISSVLQTILNKVEYGMDLKAAVEEPRIYTNSMTSYRYEKGVPEEARTKLNEMGHKFGSSPVDIGNVQSILIDRENGTFTGVADSSRNGAAIGVNLKKCEK,122.65
S437A,KKHPFSYDDYKQVDVGKDGMVATAHPLASQIGADVLKKGGNAIDAAVAIQFALNVTEPMMSGIGGGGFMMVYDAKTKDTTIIDSRERAPAGATPDMFLDENGKAIPFSERVTKGTAVGVPGTLKGLEKALDKWGTRSMKQLITPSIKLASKGFPIDSVLADAISDYKDKLSHTAAKDVFLPDGEPLKEGDTLIQKDLAKTFTAIKYKGTKAFYDGAFSKKLAETVQEFGGSMTEKDIKNFNVTIDEPIWGDYQGYHIATAPPPSSGGVFLLQMLNLLDDFKLSQYDIRSWQKYQLLAETMHLAYADRAAFAGDPEFVNVPLKGLLNPDYINARRQLINIDKVNKKPKAGDPWAYQEGSANYKQVEQPTDKQEGQTTHFTVTDRFGNVVSYTTTIEQLFGSGIMVPGYGVVLNNELTDFDAVPGGANEVQPNKRPLSAMTPTILFKNNEPVLTVGSPGGATIISSVLQTILNKVEYGMDLKAAVEEPRIYTNSMTSYRYEKGVPEEARTKLNEMGHKFGSSPVDIGNVQSILIDRENGTFTGVADSSRNGAAIGVNLKKCEK,729.46
S437G,KKHPFSYDDYKQVDVGKDGMVATAHPLASQIGADVLKKGGNAIDAAVAIQFALNVTEPMMSGIGGGGFMMVYDAKTKDTTIIDSRERAPAGATPDMFLDENGKAIPFSERVTKGTAVGVPGTLKGLEKALDKWGTRSMKQLITPSIKLASKGFPIDSVLADAISDYKDKLSHTAAKDVFLPDGEPLKEGDTLIQKDLAKTFTAIKYKGTKAFYDGAFSKKLAETVQEFGGSMTEKDIKNFNVTIDEPIWGDYQGYHIATAPPPSSGGVFLLQMLNLLDDFKLSQYDIRSWQKYQLLAETMHLAYADRAAFAGDPEFVNVPLKGLLNPDYINARRQLINIDKVNKKPKAGDPWAYQEGSANYKQVEQPTDKQEGQTTHFTVTDRFGNVVSYTTTIEQLFGSGIMVPGYGVVLNNELTDFDAVPGGANEVQPNKRPLSGMTPTILFKNNEPVLTVGSPGGATIISSVLQTILNKVEYGMDLKAAVEEPRIYTNSMTSYRYEKGVPEEARTKLNEMGHKFGSSPVDIGNVQSILIDRENGTFTGVADSSRNGAAIGVNLKKCEK,894.67