mutant,mutated_sequence,fitness_score
M1M,MRSSKIRCPGAIRVGTLVTAFGCLPHVAFAAAREAPPVTPEVLVRLADIGTMSASETTPLLSLSPDGRYVAFQVRQADPVTNLNVFRMVVKATDGATDAIDVDVGGEYLFWTIPSWGYARNAPSGANLTIQPRWSPSGTHLAYLRQDQGRVRVWRASVKGEGASPVIEDAYDIEDVQWLDDNTLIYSGRPGFVEAEAEIEREGRRGWVYDERFHPLTGARPRVLEPISIVYQVLDLKTGTRRAATPTEVARLREKPDPLRAMVGRTTFSVSRTDPQNINAPTTLVARRGEGEPVRCDEEACQNITRMWGDETANVLYFLRREGWASNEMALYRMPADALKPVRIWHATGLLQGCERQAKRLICAQESALQPRRLVTLNLTSGQMSPLYDPNPDLSRYRLPKVERLTLRNRNGIEVFSDLVLPPDYQLGTRLPLVIVQYSSRGFLRGGTGDENPILPLATAGFAVLSFHSPRSEASYQRFTSPIAQSKAEYSNWRNRWNILHTLEDLIDDLDRRGVIDPARVGLTGLSDGATTVHFGLINSHRFAAAVTSSCCTDSFTASVMNGPRISGALKAYGIETDQADDGPFWAATSFVVNASRLDTPLLIQSADEEYLGALPGFTALQQARKPVELIIYPNEHHVKWQPAHRLAVYNRTIDWFRFWLMDQSDPAPDKAAQYDRWRALRALRQKSPSPTPAP,100
W111A,MRSSKIRCPGAIRVGTLVTAFGCLPHVAFAAAREAPPVTPEVLVRLADIGTMSASETTPLLSLSPDGRYVAFQVRQADPVTNLNVFRMVVKATDGATDAIDVDVGGEYLFATIPSWGYARNAPSGANLTIQPRWSPSGTHLAYLRQDQGRVRVWRASVKGEGASPVIEDAYDIEDVQWLDDNTLIYSGRPGFVEAEAEIEREGRRGWVYDERFHPLTGARPRVLEPISIVYQVLDLKTGTRRAATPTEVARLREKPDPLRAMVGRTTFSVSRTDPQNINAPTTLVARRGEGEPVRCDEEACQNITRMWGDETANVLYFLRREGWASNEMALYRMPADALKPVRIWHATGLLQGCERQAKRLICAQESALQPRRLVTLNLTSGQMSPLYDPNPDLSRYRLPKVERLTLRNRNGIEVFSDLVLPPDYQLGTRLPLVIVQYSSRGFLRGGTGDENPILPLATAGFAVLSFHSPRSEASYQRFTSPIAQSKAEYSNWRNRWNILHTLEDLIDDLDRRGVIDPARVGLTGLSDGATTVHFGLINSHRFAAAVTSSCCTDSFTASVMNGPRISGALKAYGIETDQADDGPFWAATSFVVNASRLDTPLLIQSADEEYLGALPGFTALQQARKPVELIIYPNEHHVKWQPAHRLAVYNRTIDWFRFWLMDQSDPAPDKAAQYDRWRALRALRQKSPSPTPAP,127
W116A,MRSSKIRCPGAIRVGTLVTAFGCLPHVAFAAAREAPPVTPEVLVRLADIGTMSASETTPLLSLSPDGRYVAFQVRQADPVTNLNVFRMVVKATDGATDAIDVDVGGEYLFWTIPSAGYARNAPSGANLTIQPRWSPSGTHLAYLRQDQGRVRVWRASVKGEGASPVIEDAYDIEDVQWLDDNTLIYSGRPGFVEAEAEIEREGRRGWVYDERFHPLTGARPRVLEPISIVYQVLDLKTGTRRAATPTEVARLREKPDPLRAMVGRTTFSVSRTDPQNINAPTTLVARRGEGEPVRCDEEACQNITRMWGDETANVLYFLRREGWASNEMALYRMPADALKPVRIWHATGLLQGCERQAKRLICAQESALQPRRLVTLNLTSGQMSPLYDPNPDLSRYRLPKVERLTLRNRNGIEVFSDLVLPPDYQLGTRLPLVIVQYSSRGFLRGGTGDENPILPLATAGFAVLSFHSPRSEASYQRFTSPIAQSKAEYSNWRNRWNILHTLEDLIDDLDRRGVIDPARVGLTGLSDGATTVHFGLINSHRFAAAVTSSCCTDSFTASVMNGPRISGALKAYGIETDQADDGPFWAATSFVVNASRLDTPLLIQSADEEYLGALPGFTALQQARKPVELIIYPNEHHVKWQPAHRLAVYNRTIDWFRFWLMDQSDPAPDKAAQYDRWRALRALRQKSPSPTPAP,25
N121A,MRSSKIRCPGAIRVGTLVTAFGCLPHVAFAAAREAPPVTPEVLVRLADIGTMSASETTPLLSLSPDGRYVAFQVRQADPVTNLNVFRMVVKATDGATDAIDVDVGGEYLFWTIPSWGYARAAPSGANLTIQPRWSPSGTHLAYLRQDQGRVRVWRASVKGEGASPVIEDAYDIEDVQWLDDNTLIYSGRPGFVEAEAEIEREGRRGWVYDERFHPLTGARPRVLEPISIVYQVLDLKTGTRRAATPTEVARLREKPDPLRAMVGRTTFSVSRTDPQNINAPTTLVARRGEGEPVRCDEEACQNITRMWGDETANVLYFLRREGWASNEMALYRMPADALKPVRIWHATGLLQGCERQAKRLICAQESALQPRRLVTLNLTSGQMSPLYDPNPDLSRYRLPKVERLTLRNRNGIEVFSDLVLPPDYQLGTRLPLVIVQYSSRGFLRGGTGDENPILPLATAGFAVLSFHSPRSEASYQRFTSPIAQSKAEYSNWRNRWNILHTLEDLIDDLDRRGVIDPARVGLTGLSDGATTVHFGLINSHRFAAAVTSSCCTDSFTASVMNGPRISGALKAYGIETDQADDGPFWAATSFVVNASRLDTPLLIQSADEEYLGALPGFTALQQARKPVELIIYPNEHHVKWQPAHRLAVYNRTIDWFRFWLMDQSDPAPDKAAQYDRWRALRALRQKSPSPTPAP,200
Y438F,MRSSKIRCPGAIRVGTLVTAFGCLPHVAFAAAREAPPVTPEVLVRLADIGTMSASETTPLLSLSPDGRYVAFQVRQADPVTNLNVFRMVVKATDGATDAIDVDVGGEYLFWTIPSWGYARNAPSGANLTIQPRWSPSGTHLAYLRQDQGRVRVWRASVKGEGASPVIEDAYDIEDVQWLDDNTLIYSGRPGFVEAEAEIEREGRRGWVYDERFHPLTGARPRVLEPISIVYQVLDLKTGTRRAATPTEVARLREKPDPLRAMVGRTTFSVSRTDPQNINAPTTLVARRGEGEPVRCDEEACQNITRMWGDETANVLYFLRREGWASNEMALYRMPADALKPVRIWHATGLLQGCERQAKRLICAQESALQPRRLVTLNLTSGQMSPLYDPNPDLSRYRLPKVERLTLRNRNGIEVFSDLVLPPDYQLGTRLPLVIVQFSSRGFLRGGTGDENPILPLATAGFAVLSFHSPRSEASYQRFTSPIAQSKAEYSNWRNRWNILHTLEDLIDDLDRRGVIDPARVGLTGLSDGATTVHFGLINSHRFAAAVTSSCCTDSFTASVMNGPRISGALKAYGIETDQADDGPFWAATSFVVNASRLDTPLLIQSADEEYLGALPGFTALQQARKPVELIIYPNEHHVKWQPAHRLAVYNRTIDWFRFWLMDQSDPAPDKAAQYDRWRALRALRQKSPSPTPAP,0
N562A,MRSSKIRCPGAIRVGTLVTAFGCLPHVAFAAAREAPPVTPEVLVRLADIGTMSASETTPLLSLSPDGRYVAFQVRQADPVTNLNVFRMVVKATDGATDAIDVDVGGEYLFWTIPSWGYARNAPSGANLTIQPRWSPSGTHLAYLRQDQGRVRVWRASVKGEGASPVIEDAYDIEDVQWLDDNTLIYSGRPGFVEAEAEIEREGRRGWVYDERFHPLTGARPRVLEPISIVYQVLDLKTGTRRAATPTEVARLREKPDPLRAMVGRTTFSVSRTDPQNINAPTTLVARRGEGEPVRCDEEACQNITRMWGDETANVLYFLRREGWASNEMALYRMPADALKPVRIWHATGLLQGCERQAKRLICAQESALQPRRLVTLNLTSGQMSPLYDPNPDLSRYRLPKVERLTLRNRNGIEVFSDLVLPPDYQLGTRLPLVIVQYSSRGFLRGGTGDENPILPLATAGFAVLSFHSPRSEASYQRFTSPIAQSKAEYSNWRNRWNILHTLEDLIDDLDRRGVIDPARVGLTGLSDGATTVHFGLINSHRFAAAVTSSCCTDSFTASVMAGPRISGALKAYGIETDQADDGPFWAATSFVVNASRLDTPLLIQSADEEYLGALPGFTALQQARKPVELIIYPNEHHVKWQPAHRLAVYNRTIDWFRFWLMDQSDPAPDKAAQYDRWRALRALRQKSPSPTPAP,40