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README.md
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- **harbison_2004**: ChIP-chip transcription factor binding data with environmental
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conditions.
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### `tfbpapi`
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After [installing
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- **harbison_2004**: ChIP-chip transcription factor binding data with environmental
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conditions.
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The condition definitions are:
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- **YPD**: Rich media - Cells were grown in YPD (1% yeast extract/2% peptone/2% glucose) to an OD600 of ~0.8
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- **SM**: Amino acid starvation - Cells were grown to an OD600 of ~0.6 in synthetic complete medium followed by treatment with the inhibitor of amino acid biosynthesis sulfometuron methyl (0.2 mg/ml final) for two hours
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- **RAPA**: Nutrient deprived - Cells were grown in YPD to an OD600 of ~0.8 followed by treatment with rapamycin (100 nM final) for 20 minutes
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- **H2O2Hi**: Highly hyperoxic - Cells were grown in YPD to an OD600 of ~0.5 followed by treatment with hydrogen peroxide (4 mM final) for 30 minutes
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- **H2O2Lo**: Moderately hyperoxic - Cells were grown in YPD to an OD600 of ~0.5 followed by treatment with hydrogen peroxide (0.4 mM final) for 20 minutes
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- **Acid**: Acidic medium - Cells were grown in YPD to an OD600 of ~0.5 followed by treatment for 30 minutes with succinic acid (0.05 M final) to reach a pH of 4.0
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- **Alpha**: Mating inducing - Cells were grown in YPD to an OD600 of ~0.8 followed by treatment with the alpha factor pheromone (5 mg/ml) for 30 minutes
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- **BUT14**: Filamentation inducing (14 hours) - Cells were grown in YPD containing 1% butanol for 14 hours (corresponding to an OD600 of ~0.8)
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- **BUT90**: Filamentation inducing (90 minutes) - Cells were grown in YPD containing 1% butanol for 90 minutes (corresponding to an OD600 of ~0.8)
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- **Thi-**: Vitamin deprived medium - Cells were grown in synthetic complete medium lacking thiamin to a final OD of ~0.8
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- **GAL**: Galactose medium - Cells were grown in YEP medium supplemented with galactose (2%) to an OD600 of ~0.8
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- **HEAT**: Elevated temperature - Cells were grown in YPD at 30°C to an OD600 of ~0.5 followed by a temperature shift to 37°C for 45 minutes
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- **Pi-**: Phosphate deprived medium - Cells were grown in synthetic complete medium lacking phosphate to a final OD of ~0.8
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- **RAFF**: Raffinose medium - Cells were grown in YEP medium supplemented with raffinose (2%) to an OD600 of ~0.8
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### `tfbpapi`
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After [installing
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