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README.md
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@@ -14,28 +14,30 @@ pretty_name: "Kemmeren, 2014 Overexpression"
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size_categories:
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- 1M<n<10M
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-
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-
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temperature_celsius: 30
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growth_phase_at_harvest:
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phase: "early_mid_log"
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od600: 0.6
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od600_tolerance: 0.1
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media:
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name: "synthetic_complete"
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carbon_source:
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compound: "D-glucose"
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concentration_percent: 2.0
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components:
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- name: "amino_acid_dropout_mix"
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concentration_g_per_l: 2.0
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- name: "yeast_nitrogen_base"
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concentration_g_per_l: 6.71
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specifications:
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- "without_amino_acids"
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- "without_carbohydrate"
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- "with_ammonium_sulfate"
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cultivation_method: plate
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configs:
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- config_name: kemmeren_2014
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description: >-
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size_categories:
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- 1M<n<10M
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experimental_conditions:
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temperature_celsius: 30
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cultivation_method: plate
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growth_phase_at_harvest:
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phase: "early_mid_log"
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od600: 0.6
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od600_tolerance: 0.1
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media:
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name: synthetic_complete
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carbon_source:
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- compound: D-glucose
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# Kemmeren et al 2014: 2% D-glucose
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concentration_percent: 2
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nitrogen_source:
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- compound: yeast_nitrogen_base
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# Kemmeren et al 2014: 6.71 g/l
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concentration_percent: 0.671
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specifications:
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- without_amino_acids
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- without_carbohydrate
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- with_ammonium_sulfate
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- compound: amino_acid_dropout_mix
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# Kemmeren et al 2014: 2.0 g/l
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concentration_percent: 0.2
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configs:
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- config_name: kemmeren_2014
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description: >-
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