--- license: mit tags: - transcription-factor - binding - chipexo - genomics - biology language: - en pretty_name: Rossi ChIP-exo 2021 configs: - config_name: metadata description: Metadata describing the tagged regulator in each experiment dataset_type: metadata data_files: - split: train path: rossi_2021_metadata.parquet dataset_info: features: - name: regulator_locus_tag dtype: string description: Systematic gene name (ORF identifier) of the transcription factor - name: regulator_symbol dtype: string description: Standard gene symbol of the transcription factor - name: run_accession dtype: string description: GEO run accession identifier for the sample - name: yeastepigenome_id dtype: string description: Sample identifier used by yeastepigenome.org - config_name: genome_map description: ChIP-exo 5' tag coverage data partitioned by sample accession dataset_type: genome_map data_files: - split: train path: genome_map/*/*.parquet dataset_info: features: - name: chr dtype: string description: Chromosome name (e.g., chrI, chrII, etc.) - name: pos dtype: int32 description: Genomic position of the 5' tag - name: pileup dtype: int32 description: Depth of coverage (number of 5' tags) at this genomic position - config_name: rossi_annotated_features description: ChIP-exo regulator-target binding features with peak statistics dataset_type: annotated_features default: true metadata_fields: - regulator_locus_tag - regulator_symbol - target_locus_tag - target_symbol data_files: - split: train path: yeastepigenome_annotatedfeatures.parquet dataset_info: features: - name: sample_id dtype: int32 description: Unique identifier for each ChIP-exo experimental sample. - name: pss_id dtype: float64 description: >- Current brentlab promotersetsig table id. This will eventually be removed. - name: binding_id dtype: float64 description: Current brentlab binding table id. This will eventually be removed. - name: yeastepigenome_id dtype: float64 description: Unique identifier in the yeastepigenome database. - name: regulator_locus_tag dtype: string description: Systematic ORF name of the regulator. role: regulator_identifier - name: regulator_symbol dtype: string description: Common gene name of the regulator. role: regulator_identifier - name: target_locus_tag dtype: string description: >- The systematic ID of the feature to which the effect/pvalue is assigned. See hf/BrentLab/yeast_genome_resources role: target_identifier - name: target_symbol dtype: string description: >- The common name of the feature to which the effect/pvalue is assigned. If there is no common name, the `target_locus_tag` is used. role: target_identifier - name: n_sig_peaks dtype: float64 description: Number of peaks in the promoter region of the the target gene role: quantitative_measure - name: max_fc dtype: float64 description: >- If there are multiple peaks in the promoter region, then the maximum is reported. Otherwise, it is the fold change of the single peak in the promoter. role: quantitative_measure - name: min_pval dtype: float64 description: 'The most significant p-value among peaks for this interaction. ' role: quantitative_measure - config_name: reprocess_annotatedfeatures description: >- Annotated features reprocessed with updated peak calling methodology dataset_type: annotated_features data_files: - split: train path: creprocess_annotatedfeatures.parquet dataset_info: features: - name: regulator_locus_tag dtype: string description: Systematic gene name (ORF identifier) of the transcription factor - name: regulator_symbol dtype: string description: Standard gene symbol of the transcription factor - name: target_locus_tag dtype: string description: Systematic gene name (ORF identifier) of the target gene - name: target_symbol dtype: string description: Standard gene symbol of the target gene - name: baseMean dtype: float64 description: Average of normalized count values, dividing by size factors, taken over all samples - name: log2FoldChange dtype: float64 description: Log2 fold change between comparison and control groups - name: lfcSE dtype: float64 description: Standard error estimate for the log2 fold change estimate - name: stat dtype: float64 description: Value of the test statistic for the gene - name: pvalue dtype: float64 description: P-value of the test for the gene - name: padj dtype: float64 description: Adjusted p-value for multiple testing for the gene --- # Rossi 2021 This data is gathered from [yeastepigenome.org](https://yeastepigenome.org/). This work was published in [Rossi MJ, Kuntala PK, Lai WKM, Yamada N, Badjatia N, Mittal C, Kuzu G, Bocklund K, Farrell NP, Blanda TR, Mairose JD, Basting AV, Mistretta KS, Rocco DJ, Perkinson ES, Kellogg GD, Mahony S, Pugh BF. A high-resolution protein architecture of the budding yeast genome. Nature. 2021 Apr;592(7853):309-314. doi: 10.1038/s41586-021-03314-8. Epub 2021 Mar 10. PMID: 33692541; PMCID: PMC8035251.](https://doi.org/10.1038/s41586-021-03314-8) ## Dataset details `genome_map` is fully reprocessed data from the sequence files. I used the nf-core/chipseq pipeline, details for which can be found in `scripts/`. With those bams, I filtered the reads using `samtools` and the same settings specified in Rossi et al 2021, and then counted 5' ends using bedtools. See `scripts/count_tags.sh`. ## Data Structure ### Metadata | Field | Description | |-----------------------|-------------------------------------------------------------------| | `regulator_locus_tag` | Systematic gene name (ORF identifier) of the transcription factor | | `regulator_symbol` | Standard gene symbol of the transcription factor | | `run_accession` | GEO run accession identifier for the sample | | `yeastepigenome_id` | Sample identifier used by yeastepigenome.org | ### Genome Map | Field | Description | |----------|----------------------------------------------------------------| | `chr` | Chromosome name, ucsc (e.g., chrI, chrII, etc.) | | `pos` | Genomic position of the 5' tag | | `pileup` | Depth of coverage (number of 5' tags) at this genomic position | ## Usage The entire repository is large. It may be preferable to only retrieve specific files or partitions. You can use the metadata files to choose which files to pull. ```python from huggingface_hub import snapshot_download import duckdb import os # Download only the metadata first repo_path = snapshot_download( repo_id="BrentLab/rossi_2021", repo_type="dataset", allow_patterns="rossi_2021_metadata.parquet" ) dataset_path = os.path.join(repo_path, "rossi_2021_metadata.parquet") conn = duckdb.connect() meta_res = conn.execute("SELECT * FROM read_parquet(?) LIMIT 10", [dataset_path]).df() print(meta_res) ``` We might choose to take a look at the file with accession SRR11466106: ```python # Download only a specific sample's genome coverage data repo_path = snapshot_download( repo_id="BrentLab/rossi_2021", repo_type="dataset", allow_patterns="genome_map/accession=SRR11466106/*.parquet" ) # Query the specific partition dataset_path = os.path.join(repo_path, "genome_map") result = conn.execute("SELECT * FROM read_parquet(?) LIMIT 10", [f"{dataset_path}/**/*.parquet"]).df() print(result) ```