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<article doi="10.1016/j.molcel.2012.05.040" abstract="Bim is a proapoptotic BH3-only Bcl-2 family member. In response to death stimuli, Bim dissociates from the dynein light chain 1 (DYNLL1/LC8), where it is inactive, and can then initiate Bax/Bak-mediated mitochondria-dependent apoptosis. We found that Bim depletion increases autophagosome synthesis in cells and in vivo, and this effect is inhibited by overexpression of cell death-deficient Bim. Bim inhibits autophagy by interacting with Beclin 1, an autophagy regulator, and this interaction is facilitated by LC8. Bim bridges the Beclin 1-LC8 interaction and thereby inhibits autophagy by mislocalizing Beclin 1 to the dynein motor complex. Starvation, an autophagic stimulus, induces Bim phosphorylation, which abrogates LC8 binding to Bim, leading to dissociation of Bim and Beclin 1. Our data suggest that Bim switches locations between apoptosis-inactive/autophagy-inhibitory and apoptosis-active/autophagy-permissive sites."><fig id="4360"><title></title><label>Figure 1</label><graphic href="https://api.sourcedata.io/file.php?figure_id=4360"/><sd-panel panel_id="12816">(A) <sd-tag id="sdTag1" source="sdapi" category="entity" entity_type="gene" role="component" text="Bcl-xL" ext_ids="598" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bcl-xL</sd-tag> was cotransfected into <sd-tag id="sdTag12" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells with empty vector (IP negative control), <sd-tag id="sdTag2" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag3" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>, <sd-tag id="sdTag13" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag14" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/<sd-tag id="sdTag15" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag6" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL), <sd-tag id="sdTag16" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag17" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/<sd-tag id="sdTag18" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag7" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Puma" ext_ids="Q9BXH1" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BBC3" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Puma</sd-tag>, <sd-tag id="sdTag19" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag20" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/<sd-tag id="sdTag4" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag8" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Noxa" ext_ids="Q13794" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="PMAIP1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Noxa</sd-tag>, or <sd-tag id="sdTag11" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag>-<sd-tag id="sdTag21" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/<sd-tag id="sdTag9" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bad" ext_ids="Q92934" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BAD" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bad</sd-tag>. Anti-<sd-tag id="sdTag22" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (M2) was used for <sd-tag id="sdTag5" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>, and proteins were detected with anti-<sd-tag id="sdTag10" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bcl-xL" ext_ids="Q07817" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bcl-xL</sd-tag>, anti-<sd-tag id="sdTag23" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>, or anti-<sd-tag id="sdTag24" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bad" ext_ids="Q92934" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BAD" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bad</sd-tag>, anti-<sd-tag id="sdTag25" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (Rabbit).<graphic href="https://api.sourcedata.io/file.php?panel_id=12816"/></sd-panel><sd-panel panel_id="12817">(B) <sd-tag id="sdTag33" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag32" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O54918" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> (wild-type) was cotransfected into <sd-tag id="sdTag27" source="sdapi" category="entity" entity_type="gene" role="component" text="Bax" ext_ids="12028" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bax" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bax</sd-tag>/<sd-tag id="sdTag28" source="sdapi" category="entity" entity_type="gene" role="component" text="Bak" ext_ids="12018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bak1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bak</sd-tag> double-knockout (DKO) <sd-tag id="sdTag29" source="sdapi" category="entity" entity_type="cell_line" role="component" text="mouse embryonic fibroblasts" ext_ids="CL:2000042" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="embryonic fibroblast" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">mouse embryonic fibroblasts</sd-tag> (<sd-tag id="sdTag30" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MEFs" ext_ids="CL:2000042" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="embryonic fibroblast" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">MEFs</sd-tag>) with empty vector (IP negative control) or <sd-tag id="sdTag34" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="56208" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Becn1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag35" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>. <sd-tag id="sdTag31" source="sdapi" category="assay" entity_type="" role="" text="Immunoprecipitations" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Immunoprecipitations</sd-tag> were performed as in (A).<graphic href="https://api.sourcedata.io/file.php?panel_id=12817"/></sd-panel><sd-panel panel_id="12818">(C) <sd-tag id="sdTag38" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="O88597" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Becn1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag>-<sd-tag id="sdTag39" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> was cotransfected into <sd-tag id="sdTag40" source="sdapi" category="entity" entity_type="gene" role="component" text="Bax" ext_ids="12028" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bax" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bax</sd-tag>/<sd-tag id="sdTag41" source="sdapi" category="entity" entity_type="gene" role="component" text="Bak" ext_ids="12018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bak1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bak</sd-tag> DKO <sd-tag id="sdTag42" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MEFs" ext_ids="CL:2000042" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="embryonic fibroblast" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">MEFs</sd-tag> with empty vector (IP negative control) or <sd-tag id="sdTag43" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag44" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O54918" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> (wild-type). Anti-<sd-tag id="sdTag45" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag> was used for <sd-tag id="sdTag46" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>. ∗, antibody heavy chain.<graphic href="https://api.sourcedata.io/file.php?panel_id=12818"/></sd-panel><sd-panel panel_id="12820">(D) <sd-tag id="sdTag51" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cell lysates were subjected to <sd-tag id="sdTag49" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> antibody (negative control) and anti-<sd-tag id="sdTag52" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> antibody <sd-tag id="sdTag53" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>. Proteins were detected with anti-<sd-tag id="sdTag50" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag> and anti-<sd-tag id="sdTag54" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>. ∗∗, antibody heavy chain; ∗, antibody light chain.<graphic href="https://api.sourcedata.io/file.php?panel_id=12820"/></sd-panel><sd-panel panel_id="12822">(E) <sd-tag id="sdTag57" source="sdapi" category="entity" entity_type="gene" role="component" text="Bcl-xL" ext_ids="598" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bcl-xL</sd-tag> was cotransfected into <sd-tag id="sdTag58" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells with empty vector (IP negative control), wild-type <sd-tag id="sdTag59" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag60" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL), or <sd-tag id="sdTag61" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag56" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> L152E F159E [<sd-tag id="sdTag62" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE]. Anti-<sd-tag id="sdTag63" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> was used for <sd-tag id="sdTag64" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>. ∗, antibody light chain.<graphic href="https://api.sourcedata.io/file.php?panel_id=12822"/></sd-panel><sd-panel panel_id="12823">(F) <sd-tag id="sdTag72" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were transfected with vector, wild-type <sd-tag id="sdTag73" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag67" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>, or <sd-tag id="sdTag74" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag68" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>EE. Lysates were probed with the indicated antibodies. <sd-tag id="sdTag75" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells treated with <sd-tag id="sdTag69" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="staurosporine" ext_ids="CHEBI:15738" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="staurosporine" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">staurosporine</sd-tag> (<sd-tag id="sdTag70" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="STS" ext_ids="CHEBI:15738" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="staurosporine" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">STS</sd-tag>) for <sd-tag id="sdTag71" source="sdapi" category="time" entity_type="" role="" text="5 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">5 hr</sd-tag> were a positive control. Note that wild-type <sd-tag id="sdTag76" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> causes apoptosis, which reduces its levels compared to BimEE.<graphic href="https://api.sourcedata.io/file.php?panel_id=12823"/></sd-panel><sd-panel panel_id="12824">(G) Vector, wild-type <sd-tag id="sdTag81" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL), or <sd-tag id="sdTag86" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL) L152E F159E (<sd-tag id="sdTag82" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>EE) was transfected into <sd-tag id="sdTag87" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. Transfection efficiency was >90%. After <sd-tag id="sdTag83" source="sdapi" category="time" entity_type="" role="" text="20 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">20 hr</sd-tag>, cell viability was determined by measurement pf <sd-tag id="sdTag84" source="sdapi" category="entity" entity_type="molecule" role="assayed" text="ATP" ext_ids="CHEBI:15422" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="ATP" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">ATP</sd-tag> levels. Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12824"/></sd-panel><sd-panel panel_id="12825">(H) <sd-tag id="sdTag93" source="sdapi" category="entity" entity_type="cell_line" role="assayed" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were transfected with vector/<sd-tag id="sdTag94" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> (3:1), wild-type <sd-tag id="sdTag95" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag88" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>/<sd-tag id="sdTag96" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> (3:1), or <sd-tag id="sdTag97" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-BimEE/<sd-tag id="sdTag98" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> (3:1) (to ensure that <sd-tag id="sdTag99" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>-transfected cells also contain <sd-tag id="sdTag100" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>). After <sd-tag id="sdTag89" source="sdapi" category="time" entity_type="" role="" text="16 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">16 hr</sd-tag>, cells were stained with <sd-tag id="sdTag90" source="sdapi" category="assay" entity_type="" role="" text="Annexin V" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Annexin V</sd-tag>-<sd-tag id="sdTag91" source="sdapi" category="assay" entity_type="" role="" text="APC" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">APC</sd-tag> and analyzed by <sd-tag id="sdTag92" source="sdapi" category="assay" entity_type="" role="" text="FACS" ext_ids="BAO_0000005" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="flow cytometry" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">FACS</sd-tag>. The percentage of <sd-tag id="sdTag101" source="sdapi" category="assay" entity_type="" role="" text="APC" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">APC</sd-tag> and <sd-tag id="sdTag102" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> double-positive cells/<sd-tag id="sdTag103" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-positive cells are shown. Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12825"/></sd-panel><sd-panel panel_id="12826">(I) <sd-tag id="sdTag104" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL)/<sd-tag id="sdTag106" source="sdapi" category="entity" entity_type="gene" role="component" text="Bcl-xL" ext_ids="598" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bcl-xL</sd-tag> were cotransfected into <sd-tag id="sdTag107" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells with empty vector (IP negative control) or <sd-tag id="sdTag108" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag109" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>; <sd-tag id="sdTag110" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)-EE/<sd-tag id="sdTag116" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bcl-xL" ext_ids="Q07817" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bcl-xL</sd-tag> were also cotransfected into <sd-tag id="sdTag112" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells with <sd-tag id="sdTag105" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag>-<sd-tag id="sdTag113" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>. Anti-<sd-tag id="sdTag114" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (M2) was used for <sd-tag id="sdTag115" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>. Data are shown as mean ± SD. NS, not significant.<graphic href="https://api.sourcedata.io/file.php?panel_id=12826"/></sd-panel><sd-panel panel_id="12827">(J) <sd-tag id="sdTag118" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>EL-EE (EL)/vector (IP negative control), BimEL-EE (EL)/<sd-tag id="sdTag121" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag122" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>, BimL-EE (L)/<sd-tag id="sdTag123" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag124" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>, and <sd-tag id="sdTag120" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>S-EE (S)/<sd-tag id="sdTag119" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag>-<sd-tag id="sdTag125" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> were transfected into <sd-tag id="sdTag126" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. Anti-<sd-tag id="sdTag127" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> was used for <sd-tag id="sdTag128" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>. Data are shown as mean ± SD. ∗∗∗p < 0.001; NS, not significant.<graphic href="https://api.sourcedata.io/file.php?panel_id=12827"/></sd-panel></fig><fig id="4361"><title></title><label>Figure 2</label><graphic href="https://api.sourcedata.io/file.php?figure_id=4361"/><sd-panel panel_id="12828">(A) <sd-tag id="sdTag130" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="12125" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>−/− and <sd-tag id="sdTag136" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O54918" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>+/+ <sd-tag id="sdTag137" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MEFs" ext_ids="CL:2000042" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="embryonic fibroblast" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">MEFs</sd-tag> were cultured in 6-well dishes. Cells were treated with vehicle or <sd-tag id="sdTag131" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="Bafilomycin A1" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">Bafilomycin A1</sd-tag> (<sd-tag id="sdTag132" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="Baf" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">Baf</sd-tag>) for <sd-tag id="sdTag133" source="sdapi" category="time" entity_type="" role="" text="4 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">4 hr</sd-tag>. Blots were probed with the indicated antibodies. <sd-tag id="sdTag134" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>-II/<sd-tag id="sdTag135" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="tubulin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">tubulin</sd-tag> in wild-type control <sd-tag id="sdTag138" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MEFs" ext_ids="CL:2000042" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="embryonic fibroblast" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">MEFs</sd-tag> is set as 1. The relative value of <sd-tag id="sdTag139" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>-II/<sd-tag id="sdTag140" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="tubulin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">tubulin</sd-tag> in <sd-tag id="sdTag141" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O54918" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>-knockout <sd-tag id="sdTag142" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MEFs" ext_ids="CL:2000042" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="embryonic fibroblast" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">MEFs</sd-tag> is shown (n = 5). Data are mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12828"/></sd-panel><sd-panel panel_id="12829">(B) <sd-tag id="sdTag145" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were transfected with control siRNA and <sd-tag id="sdTag146" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> siRNA. After 48 hr, cells were treated with vehicle or <sd-tag id="sdTag147" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="Baf" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">Baf</sd-tag> for 4 hr. Blots were probed with the indicated antibodies and analyzed as described in (A). ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12829"/></sd-panel><sd-panel panel_id="12830">(C) <sd-tag id="sdTag153" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="12125" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>−/− and <sd-tag id="sdTag154" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="12125" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>+/+ <sd-tag id="sdTag155" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MEFs" ext_ids="CL:2000042" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="embryonic fibroblast" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">MEFs</sd-tag> were transfected with <sd-tag id="sdTag156" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag157" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q91VR7///Q9CQV6" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3a///Map1lc3b" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>. The percentages of cells with <sd-tag id="sdTag158" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag159" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> vesicles were assessed. Data are shown as mean ± SD. ∗∗p < 0.01.<graphic href="https://api.sourcedata.io/file.php?panel_id=12830"/></sd-panel><sd-panel panel_id="12831">(D) <sd-tag id="sdTag164" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were transfected with control plasmid (pMKO)/<sd-tag id="sdTag165" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag166" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> or pMKO-<sd-tag id="sdTag161" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>shRNA/<sd-tag id="sdTag167" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag168" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (to ensure that <sd-tag id="sdTag169" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag170" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>-positive cells also contain <sd-tag id="sdTag162" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>shRNA or control plasmid, the ratio of pMKO or pMKO-<sd-tag id="sdTag163" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>shRNA/<sd-tag id="sdTag171" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag172" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> is 3:1). The percentages of cells with <sd-tag id="sdTag173" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag174" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9GZQ8///Q9BXW4" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3B///MAP1LC3C" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> vesicles were assessed. Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12831"/></sd-panel><sd-panel panel_id="12832">(E) <sd-tag id="sdTag177" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were transfected with control plasmid (pMKO) or pMKO-<sd-tag id="sdTag176" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>shRNA. Blots were probed with the indicated antibodies. Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12832"/></sd-panel><sd-panel panel_id="12906">(F) <sd-tag id="sdTag183" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were transfected with control plasmid (pMKO)/<sd-tag id="sdTag184" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-PX or pMKO-<sd-tag id="sdTag182" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>shRNA/<sd-tag id="sdTag185" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-PX (3:1). The percentages of cells with <sd-tag id="sdTag186" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-vesicles were assessed. Data are shown as mean ± SD. ∗∗p < 0.01.<graphic href="https://api.sourcedata.io/file.php?panel_id=12906"/></sd-panel><sd-panel panel_id="12836">(G) <sd-tag id="sdTag190" source="sdapi" category="entity" entity_type="protein" role="assayed" text="DFCP1" ext_ids="Q9HBF4" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="ZFYVE1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">DFCP1</sd-tag>-<sd-tag id="sdTag192" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> cells were transfected with control siRNA or <sd-tag id="sdTag200" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> siRNA. The numbers of <sd-tag id="sdTag194" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> puncta were assessed. Data are shown as mean ± SD.∗p < 0.05.Images represent <sd-tag id="sdTag195" source="sdapi" category="entity" entity_type="protein" role="assayed" text="DFCP1" ext_ids="Q9HBF4" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="ZFYVE1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">DFCP1</sd-tag>-<sd-tag id="sdTag196" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> vesicles in cells transfected with control siRNA and <sd-tag id="sdTag191" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> siRNA. Arrows mark <sd-tag id="sdTag197" source="sdapi" category="entity" entity_type="protein" role="assayed" text="DFCP1" ext_ids="Q9HBF4" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="ZFYVE1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">DFCP1</sd-tag>-<sd-tag id="sdTag198" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag> puncta.<graphic href="https://api.sourcedata.io/file.php?panel_id=12836"/></sd-panel><sd-panel panel_id="12837">(H) <sd-tag id="sdTag202" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were treated with control siRNA or <sd-tag id="sdTag203" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> siRNA. Blots were probed with the indicated antibodies. Data are shown as mean ± SD. ∗∗p < 0.01.<graphic href="https://api.sourcedata.io/file.php?panel_id=12837"/></sd-panel><sd-panel panel_id="12838">(I) <sd-tag id="sdTag209" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL)/control siRNA, <sd-tag id="sdTag210" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)/<sd-tag id="sdTag211" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> siRNA, <sd-tag id="sdTag212" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE/control siRNA, <sd-tag id="sdTag213" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE/<sd-tag id="sdTag214" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> siRNA, vector/control siRNA, and vector/<sd-tag id="sdTag215" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> siRNA were transfected into <sd-tag id="sdTag216" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. Blots were probed with the indicated antibodies. Data are shown as mean ± SD. ∗∗p < 0.01.<graphic href="https://api.sourcedata.io/file.php?panel_id=12838"/></sd-panel><sd-panel panel_id="12839">(J) <sd-tag id="sdTag221" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were transfected with control plasmid or <sd-tag id="sdTag220" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL)EE. Blots were probed with the indicated antibodies. Data are shown as mean ± SD. ∗∗p < 0.01.<graphic href="https://api.sourcedata.io/file.php?panel_id=12839"/></sd-panel><sd-panel panel_id="12840">(K) <sd-tag id="sdTag227" source="sdapi" category="entity" entity_type="gene" role="component" text="Bax" ext_ids="12028" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bax" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bax</sd-tag>/<sd-tag id="sdTag228" source="sdapi" category="entity" entity_type="gene" role="component" text="Bak" ext_ids="12018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bak1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bak</sd-tag> DKO <sd-tag id="sdTag229" source="sdapi" category="entity" entity_type="cell_line" role="component" text="MEFs" ext_ids="CL:2000042" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="embryonic fibroblast" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://identifiers.org/cellosaurus:">MEFs</sd-tag> were transfected with control plasmid /<sd-tag id="sdTag230" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag231" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>, wild-type <sd-tag id="sdTag232" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="12125" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL)/<sd-tag id="sdTag233" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag234" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (3:1), or <sd-tag id="sdTag226" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="12125" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> (EL) EE/<sd-tag id="sdTag235" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag236" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>. The percentages of cells with <sd-tag id="sdTag237" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag238" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q91VR7///Q9CQV6" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3a///Map1lc3b" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> vesicles were assessed. Data are shown as mean ± SD. ∗∗p < 0.01.<graphic href="https://api.sourcedata.io/file.php?panel_id=12840"/></sd-panel><sd-panel panel_id="12841">(L) Two independent control vector (pcDNA3) or <sd-tag id="sdTag240" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL)EE stably expressing <sd-tag id="sdTag241" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cell clones were transfected with <sd-tag id="sdTag242" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag243" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> respectively. The percentages of cells with <sd-tag id="sdTag244" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag245" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> vesicles were assessed. Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12841"/></sd-panel><sd-panel panel_id="12842">(M) <sd-tag id="sdTag249" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were transfected with control plasmid/<sd-tag id="sdTag250" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-PX or <sd-tag id="sdTag247" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL)EE/<sd-tag id="sdTag251" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-PX (3:1). The percentages of cells with <sd-tag id="sdTag252" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-vesicles were assessed. Data are shown as mean ± SD. ∗∗p < 0.01. <sd-tag id="sdTag248" source="sdapi" category="assay" entity_type="" role="" text="Fluorescent microscope" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Fluorescent microscope</sd-tag> images show <sd-tag id="sdTag253" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-PX vesicles.<graphic href="https://api.sourcedata.io/file.php?panel_id=12842"/></sd-panel></fig><fig id="4362"><title></title><label>Figure 3</label><graphic href="https://api.sourcedata.io/file.php?figure_id=4362"/><sd-panel panel_id="12843">(A) Lysates from <sd-tag id="sdTag257" source="sdapi" category="entity" entity_type="tissue" role="component" text="spleen" ext_ids="UBERON:0002106" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="spleen" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">spleen</sd-tag>-derived <sd-tag id="sdTag256" source="sdapi" category="entity" entity_type="cell_type" role="component" text="T cells" ext_ids="CL:0000084" norm_text="" ext_dbs="CL" in_caption="True" ext_names="T cell" ext_tax_ids="" ext_tax_names="" ext_urls=" https://identifiers.org/">T cells</sd-tag> of wild-type (<sd-tag id="sdTag258" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="12125" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>+/+) and <sd-tag id="sdTag261" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O54918" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>-knockout (<sd-tag id="sdTag262" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O54918" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>−/−) <sd-tag id="sdTag259" source="sdapi" category="entity" entity_type="organism" role="component" text="mice" ext_ids="10090" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Mus musculus" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">mice</sd-tag> were <sd-tag id="sdTag260" source="sdapi" category="assay" entity_type="" role="" text="immunoblotted" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotted</sd-tag> and probed with indicated antibodies. Statistics were from three knockout and control <sd-tag id="sdTag263" source="sdapi" category="entity" entity_type="organism" role="component" text="mice" ext_ids="10090" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Mus musculus" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">mice</sd-tag>. Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12843"/></sd-panel><sd-panel panel_id="12844">(B) <sd-tag id="sdTag267" source="sdapi" category="entity" entity_type="cell_type" role="component" text="T cells" ext_ids="CL:0000084" norm_text="" ext_dbs="CL" in_caption="True" ext_names="T cell" ext_tax_ids="" ext_tax_names="" ext_urls=" https://identifiers.org/">T cells</sd-tag> from <sd-tag id="sdTag269" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O54918" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>+/+ and <sd-tag id="sdTag266" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="12125" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>−/− <sd-tag id="sdTag270" source="sdapi" category="entity" entity_type="organism" role="component" text="mice" ext_ids="10090" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Mus musculus" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">mice</sd-tag> were cultured with/without <sd-tag id="sdTag271" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="Baf" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">Baf</sd-tag> for 4 hr preceding harvest, <sd-tag id="sdTag272" source="sdapi" category="assay" entity_type="" role="" text="immunoblotted" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotted</sd-tag>, and probed with the indicated antibodies.<graphic href="https://api.sourcedata.io/file.php?panel_id=12844"/></sd-panel><sd-panel panel_id="12845">(C) <sd-tag id="sdTag275" source="sdapi" category="entity" entity_type="tissue" role="component" text="Spleen" ext_ids="UBERON:0002106" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="spleen" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">Spleen</sd-tag>-derived <sd-tag id="sdTag276" source="sdapi" category="entity" entity_type="cell_type" role="component" text="T cells" ext_ids="CL:0000084" norm_text="" ext_dbs="CL" in_caption="True" ext_names="T cell" ext_tax_ids="" ext_tax_names="" ext_urls=" https://identifiers.org/">T cells</sd-tag> from <sd-tag id="sdTag277" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="12125" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>+/+ or <sd-tag id="sdTag279" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="12125" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>−/− <sd-tag id="sdTag280" source="sdapi" category="entity" entity_type="organism" role="component" text="mice" ext_ids="10090" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Mus musculus" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">mice</sd-tag> were stained with rabbit anti-<sd-tag id="sdTag281" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9CQV6///Q91VR7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="Map1lc3b///Map1lc3a" ext_tax_ids="10090///10090" ext_tax_names="Mus musculus///Mus musculus" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> antibody. Representative <sd-tag id="sdTag278" source="sdapi" category="assay" entity_type="" role="" text="confocal images" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">confocal images</sd-tag> and quantification from triplicates (n = 100-110) are shown. Data are shown as mean ± SD. ∗∗∗p < 0.0001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12845"/></sd-panel><sd-panel panel_id="12846">(D) <sd-tag id="sdTag286" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O54918" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>+/+ and <sd-tag id="sdTag283" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="12125" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>−/− <sd-tag id="sdTag284" source="sdapi" category="entity" entity_type="organism" role="component" text="mouse" ext_ids="10090" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="mouse" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">mouse</sd-tag> <sd-tag id="sdTag285" source="sdapi" category="entity" entity_type="tissue" role="component" text="liver" ext_ids="UBERON:0002107" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="liver" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">liver</sd-tag> lysates were <sd-tag id="sdTag287" source="sdapi" category="assay" entity_type="" role="" text="immunoblotted" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotted</sd-tag> and probed with the indicated antibodies (n = 3). Data are shown as mean ± SD. ∗∗p < 0.01.<graphic href="https://api.sourcedata.io/file.php?panel_id=12846"/></sd-panel><sd-panel panel_id="12847">(E) <sd-tag id="sdTag290" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="12125" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>+/+ and <sd-tag id="sdTag291" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O54918" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>−/− <sd-tag id="sdTag292" source="sdapi" category="entity" entity_type="organism" role="component" text="mouse" ext_ids="10090" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="mouse" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">mouse</sd-tag> <sd-tag id="sdTag293" source="sdapi" category="entity" entity_type="tissue" role="component" text="liver" ext_ids="UBERON:0002107" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="liver" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">liver</sd-tag> lysates were <sd-tag id="sdTag294" source="sdapi" category="assay" entity_type="" role="" text="immunoblotted" ext_ids="BAO_0002424" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="western blot" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoblotted</sd-tag> and probed with the indicated antibodies (n = 3). Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12847"/></sd-panel><sd-panel panel_id="12848">(F) RNA from <sd-tag id="sdTag297" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="12125" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> wild-type or <sd-tag id="sdTag301" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="12125" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Bcl2l11" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>-knockout <sd-tag id="sdTag302" source="sdapi" category="entity" entity_type="organism" role="component" text="mouse" ext_ids="10090" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="mouse" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">mouse</sd-tag> <sd-tag id="sdTag304" source="sdapi" category="entity" entity_type="tissue" role="component" text="livers" ext_ids="UBERON:0002107" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="liver" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">livers</sd-tag> was analyzed by <sd-tag id="sdTag298" source="sdapi" category="assay" entity_type="" role="" text="qRT-PCR" ext_ids="BAO_0002084" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="real-time PCR " ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">qRT-PCR</sd-tag> for <sd-tag id="sdTag299" source="sdapi" category="entity" entity_type="gene" role="assayed" text="p62" ext_ids="18412" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="Sqstm1" ext_tax_ids="10090" ext_tax_names="Mus musculus" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">p62</sd-tag>/<sd-tag id="sdTag300" source="sdapi" category="entity" entity_type="gene" role="normalizing" text="beta-actin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">beta-actin</sd-tag> mRNA. The mean ± SD from three <sd-tag id="sdTag303" source="sdapi" category="entity" entity_type="organism" role="component" text="mice" ext_ids="10090" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Mus musculus" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">mice</sd-tag> per group is shown. NS, not significant.<graphic href="https://api.sourcedata.io/file.php?panel_id=12848"/></sd-panel></fig><fig id="4363"><title></title><label>Figure 4</label><graphic href="https://api.sourcedata.io/file.php?figure_id=4363"/><sd-panel panel_id="12849">(A) <sd-tag id="sdTag307" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were treated with control siRNA, <sd-tag id="sdTag305" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> siRNA + control siRNA, control siRNA + <sd-tag id="sdTag308" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag> siRNA, or <sd-tag id="sdTag309" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> siRNA + <sd-tag id="sdTag306" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag> siRNA. Blots were probed as indicated. Data are shown as mean ± SD. ∗∗p < 0.01.<graphic href="https://api.sourcedata.io/file.php?panel_id=12849"/></sd-panel><sd-panel panel_id="12850">(B) <sd-tag id="sdTag319" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were treated with control siRNA, <sd-tag id="sdTag313" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> siRNA + control siRNA, control siRNA + <sd-tag id="sdTag314" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag> siRNA, or <sd-tag id="sdTag320" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> siRNA + <sd-tag id="sdTag321" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag> siRNA. After <sd-tag id="sdTag315" source="sdapi" category="time" entity_type="" role="" text="24 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">24 hr</sd-tag>, cells were transfected with <sd-tag id="sdTag322" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-PX for <sd-tag id="sdTag325" source="sdapi" category="time" entity_type="" role="" text="24 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">24 hr</sd-tag>. <sd-tag id="sdTag323" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-PX vesicles were assessed with a <sd-tag id="sdTag316" source="sdapi" category="assay" entity_type="" role="" text="confocal microscope" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">confocal microscope</sd-tag>. Data are shown as mean ± SD. ∗∗p < 0.01. Arrows label cells with increased number/size of <sd-tag id="sdTag324" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-PX vesicles.<graphic href="https://api.sourcedata.io/file.php?panel_id=12850"/></sd-panel><sd-panel panel_id="12851">(C) <sd-tag id="sdTag330" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag326" source="sdapi" category="entity" entity_type="protein" role="reporter" text="mRFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">mRFP</sd-tag>-<sd-tag id="sdTag331" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> <sd-tag id="sdTag332" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were treated with control, <sd-tag id="sdTag327" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> siRNA, or <sd-tag id="sdTag328" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bcl-2" ext_ids="596" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bcl-2</sd-tag> siRNA and then analyzed with <sd-tag id="sdTag329" source="sdapi" category="assay" entity_type="" role="" text="Cellomics microscope" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Cellomics microscope</sd-tag>. Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12851"/></sd-panel><sd-panel panel_id="12852">(D) <sd-tag id="sdTag337" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag338" source="sdapi" category="entity" entity_type="protein" role="reporter" text="mRFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">mRFP</sd-tag>-<sd-tag id="sdTag339" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9GZQ8///Q9BXW4" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3B///MAP1LC3C" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> stable <sd-tag id="sdTag340" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were treated with control siRNA, <sd-tag id="sdTag335" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> siRNA + control siRNA, control siRNA + <sd-tag id="sdTag341" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag> siRNA, or <sd-tag id="sdTag336" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> siRNA + <sd-tag id="sdTag342" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag> siRNA and then analyzed with <sd-tag id="sdTag343" source="sdapi" category="assay" entity_type="" role="" text="Cellomics microscope" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Cellomics microscope</sd-tag> and matching confocal images are shown. Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12852"/></sd-panel></fig><fig id="4364"><title></title><label>Figure 5</label><graphic href="https://api.sourcedata.io/file.php?figure_id=4364"/><sd-panel panel_id="12853">(A) <sd-tag id="sdTag347" source="sdapi" category="entity" entity_type="gene" role="component" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL)EE/empty vector (IP negative control) or <sd-tag id="sdTag351" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE/<sd-tag id="sdTag352" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag353" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (in duplicate) were transfected into <sd-tag id="sdTag354" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. After <sd-tag id="sdTag348" source="sdapi" category="time" entity_type="" role="" text="20 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">20 hr</sd-tag>, one set of cells with <sd-tag id="sdTag355" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE/<sd-tag id="sdTag362" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag>-<sd-tag id="sdTag357" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> were starved in <sd-tag id="sdTag349" source="sdapi" category="entity" entity_type="undefined" role="component" text="HBSS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HBSS</sd-tag> for <sd-tag id="sdTag350" source="sdapi" category="time" entity_type="" role="" text="2 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">2 hr</sd-tag>. Anti-<sd-tag id="sdTag358" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> antibody (M2) was used for <sd-tag id="sdTag359" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>, and blots were probed as indicated. Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12853"/></sd-panel><sd-panel panel_id="12854">(B) Panel i: <sd-tag id="sdTag369" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were treated with control siRNA or <sd-tag id="sdTag370" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> siRNA. After <sd-tag id="sdTag363" source="sdapi" category="time" entity_type="" role="" text="48 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">48 hr</sd-tag>, one set of transfections were starved in <sd-tag id="sdTag371" source="sdapi" category="entity" entity_type="undefined" role="component" text="HBSS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HBSS</sd-tag> for <sd-tag id="sdTag364" source="sdapi" category="time" entity_type="" role="" text="2 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">2 hr</sd-tag>. Blots were probed as indicated. <sd-tag id="sdTag372" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9BXW4///Q9H492///Q9GZQ8" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3C///MAP1LC3A///MAP1LC3B" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>-II/<sd-tag id="sdTag373" source="sdapi" category="entity" entity_type="protein" role="normalizing" text="tubulin" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">tubulin</sd-tag> ratio of siRNA-transfected cells is set as 1 (n = 3). Data are shown as mean ± SD. ∗∗∗p < 0.001; NS, not significant. Panel ii: <sd-tag id="sdTag374" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were treated with control siRNA or <sd-tag id="sdTag365" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> siRNA. After <sd-tag id="sdTag366" source="sdapi" category="time" entity_type="" role="" text="48 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">48 hr</sd-tag>, one set of transfections were treated with <sd-tag id="sdTag375" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="Baf" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">Baf</sd-tag> for <sd-tag id="sdTag367" source="sdapi" category="time" entity_type="" role="" text="2 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">2 hr</sd-tag>; one set of transfections were starved and treated with <sd-tag id="sdTag376" source="sdapi" category="entity" entity_type="molecule" role="intervention" text="Baf" ext_ids="CHEBI:22689" norm_text="" ext_dbs="ChEBI" in_caption="True" ext_names="bafilomycin A1" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ebi.ac.uk/chebi/searchId.do?chebiId=">Baf</sd-tag> for <sd-tag id="sdTag368" source="sdapi" category="time" entity_type="" role="" text="2 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">2 hr</sd-tag>. Blots were probed as indicated and analyzed as in (Bi). ∗∗p < 0.01; NS, not significant.<graphic href="https://api.sourcedata.io/file.php?panel_id=12854"/></sd-panel><sd-panel panel_id="12855">(C) <sd-tag id="sdTag383" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were transfected with control plasmid /<sd-tag id="sdTag384" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag385" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (3:1), or <sd-tag id="sdTag380" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL)EE/<sd-tag id="sdTag386" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag387" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (3:1). After <sd-tag id="sdTag381" source="sdapi" category="time" entity_type="" role="" text="20 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">20 hr</sd-tag>, one set of transfected cells were starved in <sd-tag id="sdTag388" source="sdapi" category="entity" entity_type="undefined" role="component" text="HBSS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HBSS</sd-tag> for <sd-tag id="sdTag382" source="sdapi" category="time" entity_type="" role="" text="2 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">2 hr</sd-tag> and then fixed. The percentages of cells with <sd-tag id="sdTag389" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag390" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> vesicles were assessed. Data are shown as mean ± SD. ∗∗p < 0.01. NS, not significant.<graphic href="https://api.sourcedata.io/file.php?panel_id=12855"/></sd-panel><sd-panel panel_id="12856">(D) <sd-tag id="sdTag393" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>S-<sd-tag id="sdTag400" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag> binding is not sensitive to <sd-tag id="sdTag401" source="sdapi" category="entity" entity_type="undefined" role="component" text="HBSS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HBSS</sd-tag> starvation. <sd-tag id="sdTag394" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>EL-EE (EL)/vector (IP negative control), <sd-tag id="sdTag395" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>EL-EE (EL)/<sd-tag id="sdTag402" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag>-<sd-tag id="sdTag403" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (two replicates), <sd-tag id="sdTag396" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>L-EE (L)/<sd-tag id="sdTag404" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag405" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (two replicates) or <sd-tag id="sdTag397" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>S-EE (S)/<sd-tag id="sdTag406" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag407" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (two replicates) were transfected into <sd-tag id="sdTag408" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. After <sd-tag id="sdTag398" source="sdapi" category="time" entity_type="" role="" text="20 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">20 hr</sd-tag>, one of EL-<sd-tag id="sdTag409" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag410" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> transfections, one of L-Beclin-<sd-tag id="sdTag411" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> transfections and one of S-<sd-tag id="sdTag412" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag413" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> transfections were subjected to <sd-tag id="sdTag399" source="sdapi" category="time" entity_type="" role="" text="2 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">2 hr</sd-tag> <sd-tag id="sdTag414" source="sdapi" category="entity" entity_type="undefined" role="component" text="HBSS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HBSS</sd-tag> starvation. Anti-<sd-tag id="sdTag415" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (M2) was used for <sd-tag id="sdTag416" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=12856"/></sd-panel><sd-panel panel_id="12857">(E) <sd-tag id="sdTag421" source="sdapi" category="entity" entity_type="undefined" role="component" text="HBSS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HBSS</sd-tag> starvation increases <sd-tag id="sdTag422" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> T116 phosphorylation. <sd-tag id="sdTag423" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE or <sd-tag id="sdTag419" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>EE(EL)-T116A were transfected into <sd-tag id="sdTag424" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. After 20 hr, one set of <sd-tag id="sdTag425" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE transfections and one of <sd-tag id="sdTag426" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE-T116A were starved for <sd-tag id="sdTag420" source="sdapi" category="time" entity_type="" role="" text="2 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">2 hr</sd-tag> in <sd-tag id="sdTag427" source="sdapi" category="entity" entity_type="undefined" role="component" text="HBSS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HBSS</sd-tag>. Blots were probed with the indicated antibodies.<graphic href="https://api.sourcedata.io/file.php?panel_id=12857"/></sd-panel><sd-panel panel_id="12858">(F) <sd-tag id="sdTag434" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> T116 phosphorylation disables <sd-tag id="sdTag435" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>-<sd-tag id="sdTag436" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag> interaction. <sd-tag id="sdTag430" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL)EE /vector (IP negative control), <sd-tag id="sdTag437" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE/<sd-tag id="sdTag438" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag439" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (in duplicate), or <sd-tag id="sdTag440" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE-T116E (phospho-mimic, designated as T116E here)/Beclin-<sd-tag id="sdTag441" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (in duplicate) were transfected into <sd-tag id="sdTag442" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. After <sd-tag id="sdTag432" source="sdapi" category="time" entity_type="" role="" text="20 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">20 hr</sd-tag>, one set of <sd-tag id="sdTag443" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE/<sd-tag id="sdTag431" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag>-<sd-tag id="sdTag444" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> transfections and one of <sd-tag id="sdTag445" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE-T116E/<sd-tag id="sdTag446" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag447" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> transfections were starved for <sd-tag id="sdTag433" source="sdapi" category="time" entity_type="" role="" text="2 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">2 hr</sd-tag> in <sd-tag id="sdTag448" source="sdapi" category="entity" entity_type="undefined" role="component" text="HBSS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HBSS</sd-tag> (Starv). Anti-<sd-tag id="sdTag449" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (M2) was used for <sd-tag id="sdTag450" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=12858"/></sd-panel><sd-panel panel_id="12859">(G) <sd-tag id="sdTag457" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were transfected with control plasmid /<sd-tag id="sdTag458" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag459" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>, <sd-tag id="sdTag453" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL)-EE/<sd-tag id="sdTag460" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag461" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (3:1), or <sd-tag id="sdTag454" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL)-EE T116E/<sd-tag id="sdTag462" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag463" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (3:1). After <sd-tag id="sdTag455" source="sdapi" category="time" entity_type="" role="" text="20 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">20 hr</sd-tag>, one set of transfected cells were starved in <sd-tag id="sdTag464" source="sdapi" category="entity" entity_type="undefined" role="component" text="HBSS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HBSS</sd-tag> for <sd-tag id="sdTag456" source="sdapi" category="time" entity_type="" role="" text="2 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">2 hr</sd-tag> and the cells were then fixed. The percentages of cells with <sd-tag id="sdTag465" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag466" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> vesicles were assessed. Data are shown as mean ± SD. ∗∗p < 0.01; NS, not significant.<graphic href="https://api.sourcedata.io/file.php?panel_id=12859"/></sd-panel></fig><fig id="4365"><title></title><label>Figure 6</label><graphic href="https://api.sourcedata.io/file.php?figure_id=4365"/><sd-panel panel_id="12860">(A) <sd-tag id="sdTag469" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>-147 does not bind to <sd-tag id="sdTag473" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>. <sd-tag id="sdTag472" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag>-<sd-tag id="sdTag474" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/vector (IP negative control), <sd-tag id="sdTag475" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag476" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/<sd-tag id="sdTag470" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag471" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>EL-1-147aa (<sd-tag id="sdTag477" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-147), or <sd-tag id="sdTag478" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag479" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/<sd-tag id="sdTag480" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-BimEL-ΔBH3 (<sd-tag id="sdTag481" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-ΔBH3) were transfected into <sd-tag id="sdTag482" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. Anti-<sd-tag id="sdTag483" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag> (Rabbit) was used for <sd-tag id="sdTag484" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=12860"/></sd-panel><sd-panel panel_id="12861">(B) <sd-tag id="sdTag488" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>-147 is competent for <sd-tag id="sdTag486" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> binding. <sd-tag id="sdTag491" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>/vector (IP negative control), <sd-tag id="sdTag487" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC8" ext_ids="P63167" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LC8</sd-tag>/<sd-tag id="sdTag492" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag489" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>EL-1-147aa (<sd-tag id="sdTag493" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-147), or <sd-tag id="sdTag494" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>/<sd-tag id="sdTag495" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag490" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>EL-ΔBH3 (<sd-tag id="sdTag496" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-ΔBH3) were transfected into <sd-tag id="sdTag497" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. Anti-<sd-tag id="sdTag498" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag> (Rabbit) was used for <sd-tag id="sdTag499" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=12861"/></sd-panel><sd-panel panel_id="12862">(C) Mutating <sd-tag id="sdTag518" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC8" ext_ids="P63167" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LC8</sd-tag> binding consensus sites within <sd-tag id="sdTag501" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> disrupts the <sd-tag id="sdTag503" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>-<sd-tag id="sdTag504" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> interaction. <sd-tag id="sdTag505" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE/vector (IP negative control), <sd-tag id="sdTag506" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE/<sd-tag id="sdTag507" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag508" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>, <sd-tag id="sdTag509" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE-S109A, S113A, T114A (AAA)/ <sd-tag id="sdTag510" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag511" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>, or <sd-tag id="sdTag512" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE-T116E/ <sd-tag id="sdTag513" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag514" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> were transfected into <sd-tag id="sdTag515" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. Anti-<sd-tag id="sdTag516" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag> (Rabbit) was used for <sd-tag id="sdTag517" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>. Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12862"/></sd-panel><sd-panel panel_id="12863">(D) Mutating <sd-tag id="sdTag521" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> binding consensus sites within <sd-tag id="sdTag520" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> disrupts the <sd-tag id="sdTag522" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>-<sd-tag id="sdTag538" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag> interaction. <sd-tag id="sdTag524" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE/vector (IP negative control), <sd-tag id="sdTag525" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE/<sd-tag id="sdTag526" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag527" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>, <sd-tag id="sdTag528" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE-S109A, S113A, T114A (AAA)/<sd-tag id="sdTag529" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag530" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>, or <sd-tag id="sdTag531" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE-T116E/<sd-tag id="sdTag532" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag533" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> were transfected into <sd-tag id="sdTag534" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. Anti-<sd-tag id="sdTag535" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (M2) was used for <sd-tag id="sdTag536" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>. Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12863"/></sd-panel><sd-panel panel_id="12864">(E) <sd-tag id="sdTag541" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>-S does not bind to <sd-tag id="sdTag542" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>. <sd-tag id="sdTag539" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>EL-EE (EL)/vector (IP negative control), BimEL-EE (EL)/<sd-tag id="sdTag543" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag544" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>, BimL-EE (L)/<sd-tag id="sdTag545" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag546" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>, or BimS-EE (S)/<sd-tag id="sdTag547" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag540" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC8" ext_ids="P63167" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LC8</sd-tag> were transfected into <sd-tag id="sdTag548" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. Anti-<sd-tag id="sdTag549" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag> (Rabbit) was used for <sd-tag id="sdTag550" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=12864"/></sd-panel><sd-panel panel_id="12866">(G) <sd-tag id="sdTag561" source="sdapi" category="entity" entity_type="gene" role="intervention" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> enhances <sd-tag id="sdTag559" source="sdapi" category="entity" entity_type="gene" role="component" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>-<sd-tag id="sdTag552" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag> interaction in vitro. <sd-tag id="sdTag562" source="sdapi" category="entity" entity_type="protein" role="reporter" text="His" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">His</sd-tag>-<sd-tag id="sdTag553" source="sdapi" category="entity" entity_type="gene" role="component" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>, <sd-tag id="sdTag555" source="sdapi" category="entity" entity_type="protein" role="reporter" text="His" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">His</sd-tag>-<sd-tag id="sdTag563" source="sdapi" category="entity" entity_type="gene" role="intervention" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> and <sd-tag id="sdTag556" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GST" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GST</sd-tag>-<sd-tag id="sdTag554" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag> were expressed in BL21(DE3) <sd-tag id="sdTag557" source="sdapi" category="entity" entity_type="organism" role="component" text="E. coli" ext_ids="562" norm_text="" ext_dbs="NCBI taxon" in_caption="True" ext_names="Escherichia coli" ext_tax_ids="" ext_tax_names="" ext_urls="http://www.ncbi.nlm.nih.gov/taxonomy/">E. coli</sd-tag> and purified. Two micrograms of <sd-tag id="sdTag564" source="sdapi" category="entity" entity_type="protein" role="reporter" text="His" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">His</sd-tag>-<sd-tag id="sdTag565" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> was combined with 2 μg <sd-tag id="sdTag566" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GST" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GST</sd-tag> without or with 1 μg <sd-tag id="sdTag567" source="sdapi" category="entity" entity_type="protein" role="reporter" text="His" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">His</sd-tag>-<sd-tag id="sdTag580" source="sdapi" category="entity" entity_type="gene" role="intervention" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> (as controls); 2 μg <sd-tag id="sdTag568" source="sdapi" category="entity" entity_type="protein" role="reporter" text="His" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">His</sd-tag>-<sd-tag id="sdTag569" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> was combined with 2 μg <sd-tag id="sdTag570" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GST" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GST</sd-tag>-<sd-tag id="sdTag571" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag> without or with 1 μg <sd-tag id="sdTag572" source="sdapi" category="entity" entity_type="protein" role="reporter" text="His" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">His</sd-tag>-<sd-tag id="sdTag573" source="sdapi" category="entity" entity_type="gene" role="intervention" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>. The mixtures were incubated in buffer A for <sd-tag id="sdTag581" source="sdapi" category="time" entity_type="" role="" text="3 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">3 hr</sd-tag>. <sd-tag id="sdTag579" source="sdapi" category="entity" entity_type="undefined" role="component" text="Glutathione beads" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Glutathione beads</sd-tag> were then used to pull down <sd-tag id="sdTag574" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GST" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GST</sd-tag> or <sd-tag id="sdTag575" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GST" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GST</sd-tag>-<sd-tag id="sdTag576" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>. The <sd-tag id="sdTag560" source="sdapi" category="assay" entity_type="" role="" text="pulldown" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">pulldown</sd-tag> products were detected with anti-<sd-tag id="sdTag577" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> and anti-<sd-tag id="sdTag578" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=12866"/></sd-panel><sd-panel panel_id="12867">(H) <sd-tag id="sdTag585" source="sdapi" category="entity" entity_type="gene" role="intervention" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> siRNA knockdown reduces the <sd-tag id="sdTag582" source="sdapi" category="entity" entity_type="gene" role="component" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>-<sd-tag id="sdTag584" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag> interaction. Control siRNA or <sd-tag id="sdTag586" source="sdapi" category="entity" entity_type="gene" role="intervention" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> siRNA were transfected into <sd-tag id="sdTag587" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. After <sd-tag id="sdTag583" source="sdapi" category="time" entity_type="" role="" text="48 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">48 hr</sd-tag>, <sd-tag id="sdTag588" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag589" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE/vector (IP negative control) or <sd-tag id="sdTag590" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag591" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE/<sd-tag id="sdTag592" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag593" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> was transfected into control siRNA transfected <sd-tag id="sdTag594" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells; <sd-tag id="sdTag595" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag596" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>(EL)EE/<sd-tag id="sdTag597" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag598" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> was transfected into <sd-tag id="sdTag599" source="sdapi" category="entity" entity_type="gene" role="intervention" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> siRNA-transfected <sd-tag id="sdTag600" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. Anti-<sd-tag id="sdTag601" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (M2) was used for <sd-tag id="sdTag602" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>. Note that in the presence of BimEE, endogenous <sd-tag id="sdTag605" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC8" ext_ids="P63167" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LC8</sd-tag> was pulled down by <sd-tag id="sdTag604" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag> when cells were treated with control siRNA. Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12867"/></sd-panel></fig><fig id="4366"><title></title><label>Figure 7</label><graphic href="https://api.sourcedata.io/file.php?figure_id=4366"/><sd-panel panel_id="12868">(A) HeLa cells were transfected with vector (vec) /<sd-tag id="sdTag612" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag613" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9GZQ8///Q9BXW4" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3B///MAP1LC3C" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>, <sd-tag id="sdTag607" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>EL-EE (EL)/<sd-tag id="sdTag614" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag615" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9GZQ8///Q9BXW4" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3B///MAP1LC3C" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>, <sd-tag id="sdTag608" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>L-EE (L)/<sd-tag id="sdTag616" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag617" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9GZQ8///Q9BXW4" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3B///MAP1LC3C" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>, <sd-tag id="sdTag609" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>S-EE (S)/<sd-tag id="sdTag618" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag619" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9GZQ8///Q9BXW4" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3B///MAP1LC3C" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag>, or <sd-tag id="sdTag610" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>EL-EE-AAA (AAA)/<sd-tag id="sdTag620" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag621" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9GZQ8///Q9BXW4" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3B///MAP1LC3C" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (3:1). The percentages of cells with <sd-tag id="sdTag622" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag623" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9H492///Q9GZQ8///Q9BXW4" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3A///MAP1LC3B///MAP1LC3C" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> vesicles were assessed. Data are shown as mean ± SD. ∗∗∗p < 0.001; ∗∗p < 0.01; NS, not significant. Note that BimEL-EE-S109A, S113A, T114A is designated as BimEL-EE-AAA.<graphic href="https://api.sourcedata.io/file.php?panel_id=12868"/></sd-panel><sd-panel panel_id="12881">(B) <sd-tag id="sdTag629" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> bridges the <sd-tag id="sdTag630" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag631" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> interaction. <sd-tag id="sdTag626" source="sdapi" category="entity" entity_type="gene" role="component" text="Dynein light chain1" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Dynein light chain1</sd-tag> (<sd-tag id="sdTag632" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag627" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC8" ext_ids="P63167" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LC8</sd-tag>)/empty vectors (IP negative control), <sd-tag id="sdTag633" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag634" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>/<sd-tag id="sdTag635" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag636" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/empty vector, <sd-tag id="sdTag637" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag638" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>/<sd-tag id="sdTag628" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag>-<sd-tag id="sdTag639" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/BimEL-EE (EL), <sd-tag id="sdTag640" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag641" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>/<sd-tag id="sdTag642" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag643" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/<sd-tag id="sdTag656" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>L-EE (L), <sd-tag id="sdTag644" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag645" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>/<sd-tag id="sdTag646" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag647" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/<sd-tag id="sdTag657" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>S-EE (S), and <sd-tag id="sdTag648" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag649" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>/<sd-tag id="sdTag650" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag651" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/<sd-tag id="sdTag658" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>EL-EE-S109A, S113A, T114A (AAA) were transfected into <sd-tag id="sdTag652" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. Anti-<sd-tag id="sdTag653" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (M2) was used for <sd-tag id="sdTag654" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=12881"/></sd-panel><sd-panel panel_id="12882">(C) Starvation reduces the ability of <sd-tag id="sdTag665" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> to bridge the <sd-tag id="sdTag661" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag>-<sd-tag id="sdTag662" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC8" ext_ids="P63167" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">LC8</sd-tag> interaction. <sd-tag id="sdTag666" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag667" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>/empty vectors (IP negative control), <sd-tag id="sdTag668" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag669" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>/<sd-tag id="sdTag670" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-Myc/empty vector, or <sd-tag id="sdTag672" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag673" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>/<sd-tag id="sdTag674" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag675" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/<sd-tag id="sdTag676" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag663" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>EL-EE (<sd-tag id="sdTag677" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag678" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag>) (two replicates) were transfected into <sd-tag id="sdTag679" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells. After <sd-tag id="sdTag659" source="sdapi" category="time" entity_type="" role="" text="20 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">20 hr</sd-tag>, one set of cells with <sd-tag id="sdTag680" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Myc" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Myc</sd-tag>-<sd-tag id="sdTag681" source="sdapi" category="entity" entity_type="gene" role="component" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag>/<sd-tag id="sdTag682" source="sdapi" category="entity" entity_type="gene" role="component" text="Beclin 1" ext_ids="8678" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Beclin 1</sd-tag>-<sd-tag id="sdTag683" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag>/<sd-tag id="sdTag684" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag664" source="sdapi" category="entity" entity_type="protein" role="reporter" text="HA" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HA</sd-tag>-<sd-tag id="sdTag685" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Bim" ext_ids="O43521" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Bim</sd-tag> was starved in <sd-tag id="sdTag686" source="sdapi" category="entity" entity_type="undefined" role="component" text="HBSS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HBSS</sd-tag> for <sd-tag id="sdTag660" source="sdapi" category="time" entity_type="" role="" text="2 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">2 hr</sd-tag>. Anti-<sd-tag id="sdTag687" source="sdapi" category="entity" entity_type="protein" role="reporter" text="Flag" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">Flag</sd-tag> (M2) antibody was used for <sd-tag id="sdTag688" source="sdapi" category="assay" entity_type="" role="" text="immunoprecipitation" ext_ids="BAO_0002508" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="immunoprecipitation" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">immunoprecipitation</sd-tag>.<graphic href="https://api.sourcedata.io/file.php?panel_id=12882"/></sd-panel><sd-panel panel_id="12883">(D) <sd-tag id="sdTag695" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were cultured with <sd-tag id="sdTag690" source="sdapi" category="entity" entity_type="undefined" role="component" text="DMEM" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">DMEM</sd-tag> with 10% <sd-tag id="sdTag691" source="sdapi" category="entity" entity_type="tissue" role="component" text="serum" ext_ids="UBERON:0001977" norm_text="" ext_dbs="Uberon" in_caption="True" ext_names="serum" ext_tax_ids="" ext_tax_names="" ext_urls="https://www.ebi.ac.uk/ols/ontologies/uberon/terms?iri=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FUBERON_">serum</sd-tag> (Control) or starved (Starve) in <sd-tag id="sdTag696" source="sdapi" category="entity" entity_type="undefined" role="component" text="HBSS" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">HBSS</sd-tag> for 2 hr. The fixed cells were stained with <sd-tag id="sdTag692" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag> and <sd-tag id="sdTag697" source="sdapi" category="entity" entity_type="protein" role="assayed" text="tubulin" ext_ids="Q9H4B7///P68366" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="TUBB1///TUBA4A" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">tubulin</sd-tag> antibodies and analyzed by <sd-tag id="sdTag693" source="sdapi" category="assay" entity_type="" role="" text="confocal microscopy" ext_ids="BAO_0000453" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="confocal microscopy" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">confocal microscopy</sd-tag>. White boxes show the areas where <sd-tag id="sdTag694" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag> is enriched. Yellow boxes show enlarged areas.<graphic href="https://api.sourcedata.io/file.php?panel_id=12883"/></sd-panel><sd-panel panel_id="12884">(E) <sd-tag id="sdTag701" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were treated with control siRNA or <sd-tag id="sdTag702" source="sdapi" category="entity" entity_type="gene" role="intervention" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> siRNA. After <sd-tag id="sdTag699" source="sdapi" category="time" entity_type="" role="" text="24 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">24 hr</sd-tag>, cells were split. Vector/<sd-tag id="sdTag703" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag704" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> or <sd-tag id="sdTag700" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag>(EL)EE/<sd-tag id="sdTag705" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag706" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> (3:1) were transfected into the control siRNA-transfected or <sd-tag id="sdTag707" source="sdapi" category="entity" entity_type="gene" role="intervention" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> siRNA-transfected cells. The percentages of cells with <sd-tag id="sdTag708" source="sdapi" category="entity" entity_type="protein" role="reporter" text="GFP" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">GFP</sd-tag>-<sd-tag id="sdTag709" source="sdapi" category="entity" entity_type="protein" role="assayed" text="LC3" ext_ids="Q9GZQ8///Q9BXW4///Q9H492" norm_text="" ext_dbs="Uniprot///Uniprot///Uniprot" in_caption="True" ext_names="MAP1LC3B///MAP1LC3C///MAP1LC3A" ext_tax_ids="9606///9606///9606" ext_tax_names="Homo sapiens///Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">LC3</sd-tag> vesicles were assessed. Data are shown as mean ± SD. ∗∗p < 0.01; NS, not significant.<graphic href="https://api.sourcedata.io/file.php?panel_id=12884"/></sd-panel><sd-panel panel_id="12885">(F) <sd-tag id="sdTag716" source="sdapi" category="entity" entity_type="cell_line" role="component" text="HeLa" ext_ids="CVCL_0030" norm_text="" ext_dbs="CVCL" in_caption="True" ext_names="HeLa" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://identifiers.org/cellosaurus:">HeLa</sd-tag> cells were treated with control siRNA, <sd-tag id="sdTag712" source="sdapi" category="entity" entity_type="gene" role="intervention" text="Bim" ext_ids="10018" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="BCL2L11" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">Bim</sd-tag> siRNA, or <sd-tag id="sdTag717" source="sdapi" category="entity" entity_type="gene" role="intervention" text="LC8" ext_ids="8655" norm_text="" ext_dbs="NCBI gene" in_caption="True" ext_names="DYNLL1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="http://www.ncbi.nlm.nih.gov/gene/">LC8</sd-tag> siRNA for <sd-tag id="sdTag711" source="sdapi" category="time" entity_type="" role="" text="48 hr" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">48 hr</sd-tag>. Cells were then fixed in 37°C, 4% PFA for <sd-tag id="sdTag713" source="sdapi" category="time" entity_type="" role="" text="10 min" ext_ids="" norm_text="" ext_dbs="" in_caption="True" ext_names="" ext_tax_ids="" ext_tax_names="" ext_urls="">10 min</sd-tag>. Cells were stained with <sd-tag id="sdTag714" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag> and <sd-tag id="sdTag718" source="sdapi" category="entity" entity_type="protein" role="assayed" text="tubulin" ext_ids="P68366///Q9H4B7" norm_text="" ext_dbs="Uniprot///Uniprot" in_caption="True" ext_names="TUBA4A///TUBB1" ext_tax_ids="9606///9606" ext_tax_names="Homo sapiens///Homo sapiens" ext_urls="https://www.uniprot.org/uniprot////https://www.uniprot.org/uniprot/">tubulin</sd-tag> antibodies and analyzed by <sd-tag id="sdTag719" source="sdapi" category="assay" entity_type="" role="" text="confocal microscopy" ext_ids="BAO_0000453" norm_text="" ext_dbs="BAO" in_caption="True" ext_names="confocal microscopy" ext_tax_ids="" ext_tax_names="" ext_urls="https://bioportal.bioontology.org/ontologies/BAO/?p=classes&conceptid=http%3A%2F%2Fwww.bioassayontology.org%2Fbao%23">confocal microscopy</sd-tag>. White boxes/triangle show areas where <sd-tag id="sdTag715" source="sdapi" category="entity" entity_type="protein" role="assayed" text="Beclin 1" ext_ids="Q14457" norm_text="" ext_dbs="Uniprot" in_caption="True" ext_names="BECN1" ext_tax_ids="9606" ext_tax_names="Homo sapiens" ext_urls="https://www.uniprot.org/uniprot/">Beclin 1</sd-tag> is enriched. Yellow boxes show enlarged areas. Colocalizations were quantified from images in 12-15 cells with Volocity program (Colocalization coefficient Mx). Data are shown as mean ± SD. ∗∗∗p < 0.001.<graphic href="https://api.sourcedata.io/file.php?panel_id=12885"/></sd-panel></fig></article>