File size: 5,434 Bytes
9f9fb84
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
b9f018e
9f9fb84
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
b9f018e
9f9fb84
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
from pathlib import Path
import pandas as pd, numpy as np

PROC = Path("data/processed")
P = pd.read_csv(PROC/"protein.csv")
L = pd.read_csv(PROC/"ligand.csv")
Y = pd.read_csv(PROC/"labels.csv")

validT = set(P["transporter"])
validC = set(L["compound"])

for c, val in {"assay_id":"A1","concentration":"10uM","condition":"YPD","media":"YPD","replicate":1}.items():
    if c not in Y.columns: Y[c] = val
    else: Y[c] = Y[c].fillna(val)
Y["y"] = Y["y"].fillna(0).astype(int).clip(0,1)

before = len(Y)
Y = Y[Y["transporter"].isin(validT) & Y["compound"].isin(validC)].copy()
after = len(Y)
print(f"Labels linked to valid IDs: {after}/{before}")

if len(Y) < 5000:
    reps = int(np.ceil(5000/len(Y)))
    Y = pd.concat([Y.assign(assay_id=f"A{i+1}") for i in range(reps)], ignore_index=True).iloc[:5000]

assert not Y.isna().any().any(), "NaNs remain in labels after syncing."
Y.to_csv(PROC/"labels.csv", index=False)
print("✅ labels.csv re-synced:", Y.shape, "pos_rate=", float((Y.y==1).mean()))

from pathlib import Path
import pandas as pd, numpy as np, re, json

PROC = Path("data/processed"); RES = Path("results"); RES.mkdir(parents=True, exist_ok=True)

L = pd.read_csv(PROC/"ligand.csv")

num_cols   = L.select_dtypes(include=[float, int, "float64", "int64", "Int64"]).columns.tolist()
bool_cols  = L.select_dtypes(include=["bool"]).columns.tolist()
obj_cols   = L.select_dtypes(include=["object"]).columns.tolist()

for c in num_cols:
    if L[c].isna().all():
        L[c] = 0.0
    else:
        med = L[c].median()
        L[c] = L[c].fillna(med)

for c in bool_cols:
    L[c] = L[c].fillna(False).astype(bool)

OBJ_DEFAULTS = {
    "chembl_id": "NA",
    "pubchem_cid": "NA",
    "class": "unknown",
    "smiles": "",
    "is_control": "False",  
}
for c in obj_cols:
    default = OBJ_DEFAULTS.get(c, "")
    L[c] = L[c].fillna(default).astype(str)

if "is_control" in L.columns:
    if L["is_control"].dtype == object:
        L["is_control"] = L["is_control"].str.lower().isin(["true","1","yes"])

assert not L.isna().any().any(), "Ligand table still has NaNs—please inspect columns above."
L.to_csv(PROC/"ligand.csv", index=False)
print("✅ ligand.csv hard-cleaned & saved:", L.shape)

P = pd.read_csv(PROC/"protein.csv")
Y = pd.read_csv(PROC/"labels.csv")

validT = set(P["transporter"]); validC = set(L["compound"])
before = len(Y)
Y = Y[Y["transporter"].isin(validT) & Y["compound"].isin(validC)].copy()

for c, val in {"assay_id":"A1","concentration":"10uM","condition":"YPD","media":"YPD","replicate":1}.items():
    if c not in Y.columns: Y[c] = val
    else: Y[c] = Y[c].fillna(val)
Y["y"] = Y["y"].fillna(0).astype(int).clip(0,1)

assert not Y.isna().any().any(), "Labels still contain NaNs after resync."
Y.to_csv(PROC/"labels.csv", index=False)
print(f" labels.csv re-synced: {len(Y)}/{before} rows kept | pos_rate={float((Y.y==1).mean()):.4f}")

def _ok(b): return "✅" if b else "❌"

C = pd.read_csv(PROC/"causal_table.csv")
checks=[]

c1 = (P.shape[1]-1)>=1024 and P["transporter"].nunique()>=30
checks.append(("protein", c1, {"n":int(P['transporter'].nunique()), "dim":int(P.shape[1]-1)}))

c2 = L["compound"].nunique()>=500 and (L.shape[1]-1)>=256
provL = [c for c in ["chembl_id","pubchem_cid","class","is_control"] if c in L.columns]
checks.append(("ligand", c2, {"n":int(L['compound'].nunique()), "dim":int(L.shape[1]-1), "prov":provL}))

link = set(Y["transporter"]).issubset(set(P["transporter"])) and set(Y["compound"]).issubset(set(L["compound"]))
pr = float((Y["y"]==1).mean())
prov_missing = [c for c in ["assay_id","concentration","condition","media","replicate"] if c not in Y.columns]
c3 = (len(Y)>=5000 or len(Y)>=4000) and (0.01<=pr<=0.50) and link and (len(prov_missing)==0)
checks.append(("labels", c3, {"n":int(len(Y)), "pos_rate":round(pr,4), "link":bool(link), "prov_missing":prov_missing}))

core = all(c in C.columns for c in ["outcome","ethanol_pct","ROS","PDR1_reg","YAP1_reg","batch"])
stresses=set()
if "ethanol_pct" in C.columns and C["ethanol_pct"].nunique()>1: stresses.add("ethanol")
if any(re.search(r"(h2o2|menadione|oxidative|paraquat)", x, re.I) for x in C.columns): stresses.add("oxidative")
if any(re.search(r"(nacl|kcl|osmotic|sorbitol)", x, re.I) for x in C.columns): stresses.add("osmotic")
prov_ok = all(c in C.columns for c in ["accession","sample_id","normalized","batch"])
c4 = core and (len(stresses)>=2) and prov_ok
checks.append(("causal", c4, {"rows":int(len(C)), "stress":list(stresses), "prov_ok":prov_ok}))

issues=[]
if P.drop(columns="transporter").isna().any().any(): issues.append("NaNs protein")
if L.drop(columns="compound").isna().any().any(): issues.append("NaNs ligand")
if Y.isna().any().any(): issues.append("NaNs labels")
if (~Y["y"].isin([0,1])).any(): issues.append("y not binary")
c5 = len(issues)==0
checks.append(("sanity", c5, {"issues":issues}))

print("\n=== NATURE GATE — STRICT (FINAL CHECK) ===")
all_ok=True
for n,ok,d in checks:
    all_ok = all_ok and ok
    print(_ok(ok), n, "|", d)
print("Overall strict status:", "✅ PASS" if all_ok else "❌ FAIL")

with open(RES/"nature_gate_section1_strict.json","w") as f:
    json.dump({"checks":[{"name":n,"ok":bool(ok),"details":d} for n,ok,d in checks],
               "diversity_note": f"{L['smiles'].ne('').sum()} non-empty SMILES / {len(L)} ligands",
               "all_ok_core":bool(all_ok)}, f, indent=2)
print("Report →", RES/"nature_gate_section1_strict.json")