Datasets:
Restore full card; credit GenomeQA authors; clarify eukaryote_cls is within-eukaryote
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README.md
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# GenomeQA
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[GenomeQA: Benchmarking General Large Language Models for Genome Sequence Understanding](https://huggingface.co/papers/2604.05774)
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([arXiv:2604.05774](https://arxiv.org/abs/2604.05774)) by Weicai Long, Yusen Hou, Junning Feng, Houcheng Su,
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## Configurations
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| Configuration | Rows |
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| --- | ---: | --- |
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| `binary` | 2,600 |
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| `mcq` | 2,600 |
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| `binary_eukaryote`
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The
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# GenomeQA
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GenomeQA is a diagnostic question-answering benchmark for evaluating whether general-purpose language models can
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reason directly over raw nucleotide sequences. It presents English questions containing one or more DNA sequences and
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asks the model to select an answer using biological signals such as sequence composition, motifs, and motif context.
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It does not assume a specialized DNA encoder or task-specific model training.
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The original benchmark contains 5,200 examples—2,600 binary-choice questions and 2,600 four-option multiple-choice
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questions—with sequence lengths from 6 to 1,000 base pairs. It covers six task families:
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1. enhancer and promoter identification;
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2. splice-site identification;
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3. taxonomic classification;
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4. histone-mark and chromatin-state prediction;
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5. transcription-factor binding-site prediction; and
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6. transcription-factor motif prediction.
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## Source and construction
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This repository repackages the benchmark introduced in
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[GenomeQA: Benchmarking General Large Language Models for Genome Sequence Understanding](https://huggingface.co/papers/2604.05774)
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([arXiv:2604.05774](https://arxiv.org/abs/2604.05774)) by Weicai Long, Yusen Hou, Junning Feng, Houcheng Su, Shuo
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Yang, Donglin Xie, and Yanlin Zhang. **All credit for the benchmark belongs to those authors.** We repackage it in
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Parquet, add the subsets listed below, and use it to evaluate Carbon-X.
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The paper constructs GenomeQA from established genomic resources:
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| Task family | Primary source | Sequence length |
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| --- | --- | ---: |
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| Enhancer and promoter identification | EPDnew and ENCODE SCREEN | 400 bp |
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| Splice-site identification | Nucleotide Transformer downstream tasks | 600 bp |
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| Taxonomic classification | NCBI RefSeq | 1,000 bp |
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| Histone-mark prediction | Nucleotide Transformer downstream tasks | 1,000 bp |
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| TFBS prediction | ENCODE and JASPAR | 100 bp |
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| TF motif prediction | ENCODE and JASPAR | 6–20 bp |
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The authors standardize sequence windows, remove overlapping examples to reduce ambiguity, exclude sequences with
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ambiguous nucleotide bases, and convert the resulting annotations into binary-choice and multiple-choice question
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templates. Refer to the paper for the full construction procedure and source-database citations.
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## Configurations
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All configurations contain a single `train` split. The full benchmark configurations are intentionally not equal in
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size across subtasks: each contains 500 examples for the first five task families and 100 motif examples. Benchmark
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results should therefore report per-subtask accuracy and use an unweighted mean across the six subtasks when a single
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headline score is needed.
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| Configuration | Rows | Meaning |
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| --- | ---: | --- |
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| `binary` | 2,600 | Full benchmark in binary-choice format, with two ordered options per question. |
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| `mcq` | 2,600 | Full benchmark in four-option multiple-choice format. |
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| `mcq_carbon` | 2,600 | MCQ benchmark with the 500 broad taxonomic-classification (`3_species_cls`) rows replaced by 500 stratified within-eukaryote `eukaryote_cls` rows. |
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| `mcq_carbon_600` | 600 | Stratified 100-row sample from each `mcq_carbon` subtask. |
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| `binary_600` | 600 | Lower-cost binary subset with 100 examples from each of the six subtasks. |
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| `mcq_600` | 600 | Lower-cost MCQ subset with 100 examples from each of the six subtasks. |
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| `binary_eukaryote` | 170 | Binary `3_species_cls` questions whose target organism is eukaryotic. |
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| `binary_prokaryote` | 150 | Binary `3_species_cls` questions whose target organism is prokaryotic. |
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| `mcq_eukaryote` | 177 | MCQ `3_species_cls` questions whose target organism is eukaryotic. |
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| `mcq_prokaryote` | 165 | MCQ `3_species_cls` questions whose target organism is prokaryotic. |
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| `eukaryote_cls` | 1,000 | Eukaryote classification *within* eukaryotes: all four options are eukaryotic taxa, so the model must tell eukaryotic lineages apart rather than separate eukaryote from prokaryote. Auxiliary set, stored separately from the original 5,200-example benchmark. |
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The `mcq_carbon` configuration preserves the other `mcq` rows and inserts 500 `eukaryote_cls` questions at the
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original species-classification position. From source revision `ae30ecec16a7669f342f7d87439552142cdebe40`, seed 42 selects 300
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`basic_class`, 100 `reverse_search`, and 100 `odd_one_out` rows, sampling approximately half of each taxon label
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within each question type. See [`scripts/create_mcq_carbon.py`](scripts/create_mcq_carbon.py).
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The `mcq_carbon_600` configuration samples without replacement from `mcq_carbon` at revision
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`c0a7d5b8ccfe4c0d64bc3ff5dd171ff4365c16f6` using seed 42. It retains 100 rows per subtask and preserves the mix of question
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dimensions within each subtask. Within dimensions, it samples proportionally by target label where available; eukaryote odd-one-out
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questions are also stratified by majority taxon, and answer position is the final stratum. The 100 motif rows are all
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retained. See
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[`scripts/create_mcq_carbon_600.py`](scripts/create_mcq_carbon_600.py).
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The `eukaryote_cls` configuration uses the `mcq` feature schema, including JSON-serialized `metadata`.
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The `binary_600` and `mcq_600` configurations were sampled without replacement using seed 42 from source revision
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`a71ab7e0e72b8eca7acd4475744dccd0159b20be`. The 100 selected examples from each subtask were then combined and
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shuffled so the rows are not stored in subtask blocks. These subsets are balanced by task family, but not necessarily
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by every internal label or question dimension. See
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[`scripts/create_600_subsets.py`](scripts/create_600_subsets.py) for the reproducible generation script.
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The organism-specific configurations contain only rows from the `3_species_cls` subtask and preserve their source
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order. The target organism is read from `metadata`: `true` for binary `1_identity` and MCQ `basic_class` questions,
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`target` for MCQ `reverse_search` questions, and `minority` for MCQ `odd_one_out` questions because the minority class
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is the sequence the model must identify. Eukaryote and prokaryote subsets are disjoint within each answer format;
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virus rows are not included. See
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[`scripts/create_organism_subsets.py`](scripts/create_organism_subsets.py) for the reproducible filtering script.
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## Data fields
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| Field | Description |
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| --- | --- |
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| `id` | Integer identifier within the source subtask. |
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| `subtask` | Task-family identifier used for grouped evaluation. |
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| `question` | Natural-language question containing the relevant DNA sequence or sequences. |
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| `options` | Ordered list of candidate answers. |
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| `answer_index` | Zero-based index of the correct entry in `options`. |
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| `answer_text` | Correct answer text. For sequence-selection questions, this may itself be a DNA sequence. |
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| `metadata` | Serialized task-specific metadata describing the question dimension and source label. |
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## Usage
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```python
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from datasets import load_dataset
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binary = load_dataset("HuggingFaceBio/genomeqa", "binary", split="train")
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representative_mcq = load_dataset("HuggingFaceBio/genomeqa", "mcq_600", split="train")
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eukaryote_mcq = load_dataset("HuggingFaceBio/genomeqa", "mcq_eukaryote", split="train")
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```
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## Citation
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```bibtex
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@article{long2026genomeqa,
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title={GenomeQA: Benchmarking General Large Language Models for Genome Sequence Understanding},
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author={Long, Weicai and Hou, Yusen and Feng, Junning and Su, Houcheng and Yang, Shuo and Xie, Donglin and Zhang, Yanlin},
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journal={arXiv preprint arXiv:2604.05774},
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year={2026}
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}
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```
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## Reproducing
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To rebuild and publish the normalized `eukaryote_cls` configuration from the pinned source revision, run:
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```bash
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python scripts/create_eukaryote_cls.py --source-revision 18f68fabbcd43e8c227869a833027c969b39d7fe --output-dataset HuggingFaceBio/genomeqa --publish
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```
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The script preserves the source rows, converts `metadata` from Python dictionary strings to JSON, and casts the
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columns to the `mcq` feature schema. It publishes the Parquet file, this card, and the builder script in one commit.
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### mcq_carbon
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To rebuild and publish `mcq_carbon` from the pinned source revision, run:
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```bash
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python scripts/create_mcq_carbon.py --source-revision ae30ecec16a7669f342f7d87439552142cdebe40 --output-dataset HuggingFaceBio/genomeqa --publish
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```
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The script publishes the Parquet file, this card, and the builder script together.
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### mcq_carbon_600
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To rebuild and publish `mcq_carbon_600` from the pinned source revision, run:
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```bash
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python scripts/create_mcq_carbon_600.py --source-revision c0a7d5b8ccfe4c0d64bc3ff5dd171ff4365c16f6 --output-dataset HuggingFaceBio/genomeqa --seed 42 --publish
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```
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The script publishes the Parquet file, this card, and the builder script together.
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