**FORM ONE BIOLOGY** ***By the end of form one work, the learner should be able*** ***to:***

***Define***Biology ***List***the branches of Biology ***Explain***the importance of Biology ***State***and***explain***some of the characteristics of organisms ***State***and***explain***some of the general characteristics of organisms ***Explain***the external features of plants and animals ***Write down***the***difference***between plants and animals ***Define***classification  Use the magnifying lens to***observe***the external features of plants/ animals ***Record***observations of the main external features of plant leaf form ***Draw***different types of leaf forms ***Observe***,***record***and***draw***the main external features of plants ***Observe***,***record***and***draw***the main external features of animals ***State t***he necessity and significance of classification ***Name t***he major units of classification  Name the five kingdoms of living things  List the taxonomic units in plant and animal kingdoms  Classify maize and human beings  Define Binomial nomenclature  State the principles of Binomial nomenclature In naming organisms  Use collecting nets, cutting instructions instruments and hand lens  Preserve collected specimen  Observe and group collected and preserved specimen according to their similarities  Define a cell  Draw and label the light microscope  Identify parts of the light microscope and state their functions  Describe how to care for a light microscope  Describe how a light microscope is used  Draw and label plant and animal cells as seen under a light microscope  Calculate the magnification of objects as seen under a light microscope  Observe a prepared slide under a light microscope  Prepare temporary slide of onion epidermis and observe it under a light microscope  Draw and label plant and animal cells as seen under electron microscope  Describe the structure and function of the cell  Cell membrane  Cytoplasm

 Describe the structure and function of the cell organelles  Estimate the size of a cell as seen in the field of view of a microscope  Write down the differences between plants and animal cells  Write down similarities between plant and animal cells  List down specialized plant and animal cells  State the modifications and functions of specialized cells  Define tissues, organs and organ systems  Give examples of tissues organs and organ systems  Define the term cell physiology  Describe the structure and properties of cell membrane  Define diffusion  Carry out experiments to demonstrate

 diffusion in liquids  diffusion in gasses

 Explain the factors affecting diffusion  Explain the role of diffusion in living things  Define osmosis  Describe movement of water molecules across semi-permeable membrane  define and describe the terms used in the study of osmosis such as:

 Osmotic pressure  Osmotic potential  Isotonic solution  Hypertonic solution  Hypotonic solution  Turgor pressure  Hemolysis  Wall pressure  Plasmolysis  Deplasmolysis  carry out an experiment on selective permeability of membrane  State factors affecting osmosis  Explain the role of osmosis in organisms  Explain the factors affecting osmosis  Describe what happens when a plant cell is placed in a hypertonic, hypotonic or

isotonic solution

 Carry out an experiment to show plasmolysis in epidermal cells of an onion bulb  Describe osmosis of animal cells in a hypertonic solution  List down factors affecting active transport  Define active transport  Define the role of active transport in living things  Define nutrition  Write down the importance of nutrition  List down the modes of feeding in organisms  Draw and label the external structure of a leaf  Draw and label the internal structure of the leaf  Name the parts of a leaf  State the functions of the parts of a leaf  Define photosynthesis  Draw and label the chloroplast  Describe the process of photosynthesis  List down the importance of photosynthesis  Explain some of the factors influencing photosynthesis  Explain the factors affecting photosynthesis  Explain how the leaf is adapted to the process of photosynthesis  Test the presence of starch in a green leaf  Investigate whether chlorophyll is necessary for photosynthesis  Investigate whether light is necessary for photosynthesis  carry out an experiment to investigate whether

 Carbon (IV) oxide is necessary for photosynthesis

 Oxygen is produced during photosynthesis

 Define Chemicals of life  List down types of carbohydrates  Write down properties and functions of monosaccharaides  Define disaccharides  List properties and functions of disaccharides  Define hydrolysis and condensation  Define polysaccharides and lipids  Write down the properties of polysaccharides and lipids  carry out tests on  Starch  Reducing sugars  Non-reducing sugar  Lipids  Proteins  Vitamin c

 Write down the properties and functions of proteins  Distinguish between carbohydrates, proteins and lipids  Define enzymes  Write down the properties and functions of enzymes  Know the naming of the enzymes and their substrates  Explain the importance of enzymes  carry out an experiment on  Effect of temperature on enzymes  Effects of enzyme concentration on the rate of a reaction  Effect of PH on enzyme activities

 Define hetetrophism  List down the different modes of heterotrophism and describe them  Define dentition  Draw and label different types of teeth  Describe the structure of a tooth  Identify different types of teeth  Describe the adaptations of the teeth to their functions  Define dental formulae  Describe and write down the dental formulae of herbivore carnivore and omnivore

 Write down the definition of herbivores, carnivores and omnivores  Explain the adaptations of dental formulae in various groups of animals, to their

mode of feeding  Draw and label the internal structure of different types of teeth  Write down the functions of the different parts of the internal structure of teeth  Name and discuss common dental diseases  Write down the adaptations of herbivores to their mode of feeding  Write down the adaptations of carnivores to their modes of feeding  Identify various organs associated with the digestive system of a rabbit  Draw and label parts of the human digestive system  Describe the regions of the alimentary canal of human digestive system  Explain the functions of the human digestive system  Describe the various regions of the human alimentary canal and their functions  Describe how the ileum is adapted to its function  Analyze the food content in the alimentary canal of a herbivore  Carry out an experiment on the breakdown of starch by diastase enzymes  Describe how the ileum is farther adapted to its functions  Explain the end products of the digestion of various food  Explain the function of the colon  Explain the process of assimilation of food substances  Write down the summary of chemical digestion in alimentary canal  Write down the importance of vitamins in human nutrition  Write down the sources of vitamins  State deficiency diseases of various vitamins  Write down the importance of mineral salts in human nutrition  State the source of mineral salts  State the deficiency diseases of mineral salts **Introduction To Biology**  Biology derived from Greek words-BIOS meaning LIFE and LOGOS meaning STUDY or

KNOWLEDGE.

 Biology means "life knowledge".  It is the study of living things/organisms.

***Branches of Biology***

 Botany - study of plants.  Zoology - study of animals.  Microbiology - study' of microscopic organisms.  Morphology - study of external structure of organisms.  Anatomy - study of internal structure of organisms.  Physiology - study of the functioning or working of the cells or body.  Biochemistry - study of the chemistry of materials in living organisms.  Cytology - study of cells.  Genetics - study of inheritance.  Ecology- study of the relationship between organisms and their environment.  Taxonomy - sorting out of organisms into groups.  Histology - study of fine structure of tissues.  Virology - study of viruses.  Bacteriology - study of bacteria.  Entomology - study of insects.  Ichthyology - study of fish.

***Importance of Biology***

 One learns about the functioning of the human body.  One understands the developmental changes that take place in the body.  It contributes immensely to improved life.  It enables one to enter careers such as:

 Medicine,  Nutrition,  Public Health,  Dentistry,  Agriculture  Environmental Studies.  Teaching ***Characteristics of Living Things*** ***Life defined through observations of activities carried out by living things;***

**Nutrition **

 Nutrition is the processes by which food/nutrients are acquired/made and

utilized by living organisms.

 Green plants and certain bacteria make their own food.  All other organisms feed on complex organic materials.

**Respiration **

 This is the breakdown of food to provide energy.  The energy released is used for various activities in the organism.

**Gaseous Exchange **Process throw which respiratory gases(CO2&O2) are taken in and

out through a respiratory surface.

**Excretion **

 Excretion is the removal of metabolic wastes from the body.  Substances like urea, carbon dioxide (Carbon (IV) oxide).  These substances are poisonous if allowed to accumulate in the body.

**Growth and Development **

 Growth means irreversible change in size.  All organisms increase in size that is, they grow.  Development is irreversible change in complexity.  As they do so, they also become differentiated in form.

**Reproduction-**Reproduction is the formation of new individuals of a species to

ensure continued existence of a species and growth of its population. **Irritability **

 The ability of organisms to detect and respond to changes in the environment.

This is of great survival value to the organism.

**Movement **

 Is the progressive change in position from one place to another.  Some organisms are sessile (i.e. fixed to the substratum).  The majority of plants move only certain parts.

***Collection and Observation of Organisms*** Biology as a practical subject is learnt through humane handling of organisms. Materials needed for collection of organisms:-

 Knives to cut portions of plant stem/root or uproot.  Polythene bags to put the collected plant or specimens.  Insect collecting jars.  Insect killing jars.  Hand gloves.  Sweep nets  Pooters  Traps

***Observation of Organisms***

 Observe the plant/animal in its natural habitat before collecting.   What does it feed on?

Identify the exact place -on surface, under rock, on tree trunk, on branches.  How does it interact with other animals and the environment?  How many of that kind of plant or animal are in a particular place?  Plant specimens placed on the bench and sorted out into;-

***seeds/stems/roots/leaves/fruits.***

 Animal specimens may be left inside polythene bags if transparent.  Others (killed ones) are put in petri dishes.  Use hand lens to observe the external features of small animals.

***Presenting the Results of Observations***

 Organisms are observed and important features noted down: colour, texture hard or

soft; if hairy or not. Size is measured or estimated. **Biological Drawings**- It is necessary to draw some of the organisms. In making a biological drawing, magnification (enlargement) is noted. Indicate the magnification of your drawing. i.e how many times the drawing     is larger/smaller than the actual specimen

MG=length of drawing/length specimen

***How to Draw***

 Several drawings of one organism may be necessary to represent all features

observed, e.g.

 Anterior view of grasshopper shows all mouth parts properly, but not all limbs.  Lateral (side) view shows all the legs. ***Collection, Observation and Recording of Organisms*** ***Collection***

 Plants and animals collected from the environment, near school or within school

compound using nets, bottles and gloves.

 Animals collected include:-arthropods, earthworms and small vertebrates like

lizards/chameleons/ rodents.

 Place in polythene bags and take to the laboratory.  Stinging/poisonous insects killed using ether.  Other animals are observed live and returned to their natural habitat.  Plant specimen collected include:- leaves, flowers and whole plants.  Observations are made to show the following:-

 Plants have roots, stems, leaves and flowers.  Animals have legs, hair, hard outer covering, feathers, eyes, mouth, limbs and

other appendages, ***The differences between animals and plants collected.***

***Comparison Between Plants And Animals***

*Plants* *Animal*

Plants are fixed in position and do not" move. Most animals move in search of food, shelter and mates. l. l.

2. Respond slowly to stimuli.

2. Respond quickly to stimuli. 3. Cells have no cell walls only a cell 4. Animals feed on already made food.

3. Cells have cellulose cell walls. membrane 4. Plants make their own food from simple

 materials such as CO2 and water using

light energy. **Classification I**

***Introduction***

 Classification is putting organisms into groups.  Classification is based on the study of external characteristics of organisms.  It involves detailed observation of structure and functions of organisms.  Organisms with similar characteristics are put in one group.  Differences in structure are used to distinguish one group from another.  The magnifying lens is an instrument that assists in the observation of fine structure

e.g. hairs by enlarging them.

***Using a Magnifying Lens***

 A specimen is placed on the bench or held by hand,  Then the magnifying lens is moved towards the eye until the object is dearly focused

and an enlarged image is seen.

 The magnification can be worked out as follows: length of the drawing Magnification = length of the specimen

***Note: magnification has no units.***

***Nececity/need for Classification***

 To be able to identify organisms into their taxonomic groups.  To enable easier and systematic study of organisms.  To show evolutionary relationships in organisms.

***Major Units of Classification (Taxonomic Groups)***

 Taxonomy is the study of the characteristics of organisms for the purpose of

classifying them.

 The groups are Taxa (singular Taxon).

***The taxonomic groups include:*** ***Species:***This is the smallest unit of classification. Organisms of the same species resemble each other. The number of chromosomes in their cells is the same. Members of a species interbreed to produce fertile offspring.

***Genus (plural genera**):*A genus is made up of a number of species that share several characteristics. Members of a genus cannot interbreed and if they do, the offspring are infertile.

***Family:***A family is made up of a number of genera that share several characteristics. ***Order***: A number of families with common characteristics make an order. **Class**: Orders that share a number of characteristics make up a class. ***Phylum/Division:***A number of classes with similar characteristics make up a

phylum (plural phyla) in animals. In plants this is called a division.

***Kingdom:***This is made up of several phyla (in animals) or divisions (in plants). It is

the largest taxonomic unit in classification.

***Kingdoms*** Living organisms are classified into five kingdoms;

 Monera,  Protoctista,  Fungi,  Plantae  Animalia. ***Kingdom Fungi***

 Some are unicellular while others are multicellular.  They have no chlorophyll.  Most are saprophytic e.g. yeasts, moulds and mushrooms.  A few are parasitic e.g.***Puccinia graminae.***

***Kingdom Monera (Prokaryota)***

 These are very small unicellular organisms.  They lack a nuclear membrane  do not have any bound membrane organelles.  Hence the name Prokaryota.  They are mainly bacteria, e.g.***Vibrio cholerae**.*

***Kingdom Protoctista***

 They are unicellular organisms.  Their nucleus and organelles are surrounded by membranes (eukaryotic).  They include algae, slime moulds - fungi-like and protozoa

***Kingdom Plantae***

 They are all multicellular.  They contain chlorophyll and are all autotrophic.  They include; Bryophyta (mossplant), Pteridophyta (ferns) and Spermatophyta (seed

bearing plants).

***Kingdom Animalia***

 These are all multicellular and heterotrophic.  Examples are annelida (earthworms), mollusca (snails),athropoda, chordata .  Example of Arthropods are ticks, butterflies.  Members of Chordata are fish, frogs and humans.

***External Features of Organisms***

***In plants we should look for:-***

 Spore capsule and rhizoids in moss plants.  Sori and fronds in ferns.  Stem, leaves, roots, flowers, fruits and seeds in plants.

***In animals, some important features to look for are:***

 Segmentation, presence of limbs and, number of body parts, presence and number

of antennae. These are found in phylum arthropoda:

 Visceral clefts, notochord, nerve tube, fur or hair, scales, fins, mammary glands,

feathers and wings.

 These are found in chordata.

***Binomial Nomenclature***

 Organisms are known by their local names.  Scientists use scientific names to be able to communicate easily among themselves.  This method of naming uses two names, and is called Binomial nomenclature.  The first name is the name of the genus: (generic name) which starts with a capital

letter.

 The second name is the name of the species (specific name) which starts with a small

letter.

 The two names are underlined or written in italics.  Man belongs to the genus*Homo,*and the species,*sapiens.*  The scientific name of man is therefore*Homo sapiens.*  Maize belongs to the genus*Zea,*and the species*mays.*  The scientific name of maize is*Zea mays.* ***Practical Activities***

 Use of Collecting Nets, Cutting Instruments and Hand Lens.  Forceps are used to collect crawling and slow moving animals.  Sweep nets are used to catch flying insects.  Cutting instrument like scapel is used to cut specimen e.g. making sections.  Hand lens is used to magnify small plants and animals.  Drawing of the magnified organism are made and the linear magnification of each

calculated.

***Collection and Detailed Observation of Small Plants and Animals*** ***e.g. moss, ferns, bean.***

Look for the following:  Moss plants: Rhizoids and spore capsules.  Fern plants: Rhizomes with adventitious roots; large leaves (fronds) with Sori

(clusters of sporangia).

 Seed plants: Tree/shrub (woody) or non-woody (herbs) e.g. bean.  Root system - fibrous, adventitious and tap root.  Stem - position and length of interrnodes.  Type of leaves - simple or compound; arranged as alternate, opposite or whorled.  Flower - colour, number of parts, size and relative position of each:  Fruits - freshy or dry; edible or not edible.  Seeds - monocotyledonous or dicotyledonous.

**Small animals e.g. earthworms, tick, grasshopper, butterfly, beetles.** Observe these animals to see:

 Number of legs.  Presence or absence of wings.  Number of antennae.  Body covering.  Body parts. ***THE CELL***

***Introduction***

 The cell is the basic unit of an organism.  All living organisms are made up of cells.  Some organisms are made up of one cell and others are said to be multicellular.  Other organisms are made of many cells and are said to be multicellular.  Cells are too little to see with the naked eye.  They can only be seen with the aid of a microscope.

***The microscope*** The microscope is used to magnify objects. ***Magnification***

 The magnifying power is usually inscribed on the lens.  To find out how many times a specimen is magnified, the magnifying power of the

objective lens is multiplied by that of the eye piece lens. If the eye piece magnification lens is x10 and the objective lens is x4, the total magnification is x40.

 Magnification has no units.  It should always have the multiplication sign.e.g.x40

**Microscope parts and their functions**

*Parts* *Function(s)*

Has a lens which contributes to the magnification of the object under view. Moves the body tube up and down for long distances and it brings the image into focus.

Eye piece Coarse adjustment knob Fine adjustment knob Moves the body tube and brings the image into fine focus. Body tube

Holds the eye piece and the revolving nose piece. It directs light from objective lenses to the eye piece lens. Ji

Revolving nose piece Holds and brings objective lenses into position. Contributes to the magnification of the object. Objective lens It is for handling the microscope and also tilting it. Arm/limb Is the flat platform onto which the slide with the object is placed. Stage They hold the slide firmly onto the stage. Clips Concentrates light onto the object. Condenser ~egulates the amount of light passing through the object. Diaphragm Reflects light into the condenser. Mirror Fixes the arm to the base and allows for tilting of the arm. Hinge screw Provides support to the microscope. Base/stand

***To View the Object***

 Turn the low power objective lens until it clicks into position.  Looking through the eye piece, ensure that enough light is passing through by

adjusting the mirror.

 This is indicated by a bright circular area known as the field of view.  Place the slide containing the specimen on stage and clip it into position.  Make sure that the specimen is in the centre of the field of view.  Using the coarse adjustment knob, bring the low power objective lens to the lowest

point.

 Turn the knob gently until the specimen comes into focus.  If finer details are required, use the fine adjustment knob.  When using high power objective always move the fine adjustment knob upwards.

***Care of a Microscope***

 Great care should be taken when handling it.  Keep it away from the edge of the bench when using it.  Always hold it with both hands when moving it in the laboratory.  Clean the lenses with special lens cleaning paper.  Make sure that the low power objective clicks in position in line with eye piece lens

before and after use.

 Store the microscope in a dust-proof place free of moisture.

***Cell Structure as Seen Through the Light Microscope***

***The cell as seen above has the following:*** ***Cell membrane (Plasma membrane):***

 This is a thin membrane enclosing cell contents.  It controls the movement of substances into and out of the cell.

***Cytoplasm***:

 This is a jelly-like substance in which chemical processes are carried out.  Scattered all over the cytoplasm are small structures called organelles.  Like an animal cell, the plant cell has a cell membrane, cytoplasm and a nucleus.

***vacuole.***

 Plant cells have permanent, central**vacuole.**It contains cell sap where sugars and

salts are stored.

***Cell wall:***

 This is the outermost boundary of a plant cell.   Between the cells is a middle lamella made of calcium pectate.

It is made of cellulose.

***Chloroplasts;***

 With special staining techniques it is possible to observe**chloroplasts.**  These are structures which contain chlorophyll, the green pigment responsible for

trapping light for photosynthesis.

***The Electron Microscope (EM)***

 Capable of magnifying up to 500,000 times.  The specimen is mounted in vacuum chamber through which an electron beam is

directed.

 The image is projected on to a photographic plate.  The major**disadvantage**of the electron microscope is that it**cannot be used to**

**observe living objects**.

 However, it provides a**higher magnification**and**resolution**(ability to see close points as separate) than the light microscope so that specimen can be observed in more detail.

***Cell Structure as Seen Through Electron Microscope***

***The Plasma Membrane***

 Under the electron microscope, the plasma membrane is seen as a double layer.  This consists of a lipid layer sandwiched between two protein layers.  This arrangement is known as the unit membrane and the shows two lipid layers with

proteins within.

 Substances are transported across the membrane by active transport and diffusion.

***The Endoplasmic Reticulum (ER)***

 This is a network of tubular structures extending throughout the cytoplasm of the

cell. It serves as a network of pathways through which materials are transported from one part of the cell to the other.

 An ER encrusted with ribosomes it is referred to as**rough endoplasmic reticulum**.  An ER that lacks ribosomes is referred to as**smooth endoplasmic reticulum.**  The rough endoplasmic reticulum transports**proteins**while the smooth endoplasmic

reticulum transports**lipids.**

***The Ribosomes***

 These are small spherical structures attached to the ER.  They consist of protein and ribonucleic acid (RNA).  They act as sites for the synthesis of proteins. ***Goigi Bodies***

 Golgi bodies are thin, plate-like sacs arranged in stacks and distributed randomly in the

cytoplasm.

 Their function is packaging and transportation of glycol-proteins.  They also produce lysosomes.

***Mitochondria***

 Each mitochondrion is a rod-shaped organelle.  Made up of a smooth outer membrane and a folded inner membrane.  The foldings of the inner membrane are called**cristae.**  They increase the surface area for respiration.  The inner compartments called the matrix.  Mitochondria are the sites of cellular respiration, where energy is produced.

***Lysosomes***

 These are vesicles containing hydrolytic enzymes.  They are involved in the breakdown of micro-organisms, foreign macromolecules and

damaged or worn-out cells and organelles ..

***The Nucleus***

 The nucle s is surrounded by a nuclear membrane which is a unit membrane.  The nuclear membrane has pores through which materials can move to the

surrounding cytoplasm.

 The nucleus contains proteins and nucleic acid deoxyribonucleic acid (DNA) and RNA.  The chromosomes are found in the nucleus.  They are the carriers of the genetic information of the cell.  The nucleolus is also located in the nucleus but it is only visible during the non-dividing phase of

the cell. ***The Chloroplasts***

 These are found only in photosynthetic cells.  Each chloroplast consists of an outer unit. membrane enclosing a series of

interconnected membranes called lamellae.

 At various points along their length the lamellae form stacks of disc like structures

called grana.

 The lamellae are embedded in a granular material called the stroma.  The chloroplasts are sites of photosynthesis.  The light reaction takes place in the lamellae while the dark reactions take place in the

stroma. ***Comparison between animal cell and plant cell*** ***Animal Cell***        ***Plant Cell***  Has a cell wall and a cell membrane.  Nucleus at periphery.  Have  Has a large central vacuole. chloroplasts.  Are usually large.  Are regular in shape.  Has no centriole. Has cell membrane only. Nucleus at the center. Have no chloroplasts. Has no vacuoles, they are small and scattered. Are usually small. Irregular in shape. Has centrioles. ***Cell Specialisation*** Cells are specialised to perform different functions in both plants and animals. **Example;**

 Palisade cells have many chloroplasts for photosynthesis.  Root hair cells are long and thin to absorb water from the soil.  Red blood cells have haemoglobin which transports oxygen.  Sperm cells have a tail to swim to the egg.  Multicellular organisms cells that perform the same function are grouped together to form a tissue.  Each tissue is therefore made up of cells that are specialised to carry out a particular function.

***Animal Tissues- Examples of animal tissues***

 Type of Tissue l. Epithelial Tissue

Functions Covering. allowing movement of materials Covering of internal organs. lining for Secretion. absorption e.g. in the body cavity. Covering surfaces, protection e.g. the alimentary canal. skin. Absoption e.g. in the kidney tubules. Contraction, bringing about Contract and allow movement. movement of body parts.

Characteristics

 (a) Squamous (b) Columnar epithelium (c) stratified epithelium epithelium

Thin flat cells. Cells that are longer than they Several layers of epithelial cells are wide. squamous. cuboidal or (either cube like cells. columnar). Contists of units called Are multicleated; have myofibrils. controlled by voluntary transverse striations; Are spindle-shaped. nervous system. controlled by involuntary mononucleated; contract rhythmically; are nervous system. (ability to contract is within) myogenic Cells that produce hard materials.

 (d) Cuboidal 2. Muscular Tissue (a) Striated epithelium

 voluntary (skeletal or (b) Smooth muscle) involuntary (visceral or muscle)

3. Supporting Tissue Support the body. provide a rigid

framework, protect soft tissue. Transport of materials. protection against disease. Receive stimuli and transmit impulses; Consists of cells called co-ordinate body activities

A complex tissue consisting of three types of cells suspended in a fluid medium (Plasma) neurones which are interconnected axons to enable transmission through of impulses ***Plant Tissues*** ***Example of plant tissues***

***Type of Tissue*** L*Meristematic*Undergo division and cause growth, e.g. increase in length and girth

***Functions*** ***Characteristics***

Small thin-walled celis, contain a lot of cytoplasm; found mostly at the tip of shoots and roots. Thin walled cells; vary in shape and size; many intercellular spaces. Photosynthesis gaseous exchange; support; storage.

*2. Parenchyma* Thickened walls; no intercellular

spaces; found in cortex of stems.

Vary in shape; thick cell walls; are usually dead. Tubular vessels and trancheids joined end to end. Sieve elements joined to each other through sieve pores. *4. Sclerenchyma*Strengthening.

5.*Vascular* (a) Xylem (b) Phloem Transport materials. Transport of water and mineral Transport of organic materials salts. (manufactured food).

***Organs***

 An organ is made up of different tissues  e.g. the heart, lungs, kidneys and the brain in animals and roots, stems and leaves in

plants. ***organism***  Different organ systems form an organism.

***Practical Activities*** Observation and Identification of parts of a light microscope and their functions

 A light microscope is provided.  Various parts are identified and observed.  Drawing and labelling of the microscope is done.  Functions of the parts of the mircroscope are stated.  Calculations of total magnification done using the formula.

**Eye piece lens maginification**x**objective lens rnaginification.**

***Preparation and Observation of Temporary Slides of Plant Cells***

 A piece of epidermis is made from the fleshy leaf of an onion bulb. It is placed on a

microscope slide and a drop of water added.

 A drop of iodine is added and a cover slip placed on top.  Observations are made, under low and medium power objective.  The cell wall and nucleus stain darker than other parts.  A labelled drawing is made.  The following are noted:**Nucleus, cell wall, cytoplasm and cell membrane.**

***Observation of permanent slides of animal cells***

 Permanent slides of animal cells are obtained e.g, of cheek cells, nerve cells and

muscle cells.

 The slide is mounted on the microscope and observations made under low power

and medium power objectives.  Labelled drawings of the cells are made.  A comparison between plant and animal cell is made.

***Observation and Estimation of Cell Size and Calculation of Magnification of*** ***Plant Cells.***

 Using the low power objective, a transparent ruler is placed on the stage of the

microscope.

 An estimation of the diameter of the field of view is made in millimeters.  This is converted into micrometres (1mm=1000u)  A prepared slide of onion epidermal cells is mounted.  The cells across the centre of the field of view are counted from left and right and

top to bottom.

 The diameter of field of view is divided by the number of cells lying lengthwise to give

an estimate of the length and width of each cell. **Cell Physiology**

***Meaning of cell physiology***

 The term physiology refers to the functions that occur in living organisms.  Cell physiology refers to the process through which substances move across the cell

membrane.

 Several physiological processes take place inside the cell.e.g. respiration.  Oxygen and glucose required enter the cell while carbon (IV) oxide and water

produced leave the cell through the cell membrane.

***Structure and properties of cell membrane***

 The cell membrane is the protective barrier that shelter cellular contents.  Movement of all substances into and out of the cells takes place across the cell

membrane. It is made up of protein and lipid molecules.   Lipid molecules have phosphate group attached to it on one end.  They are then referred to phospholipids.  The phospholipids are arranged to form a double layer.  The ends with phosphate group face outwards.   Some of these proteins act as carrier molecules that channel some material in and

the proteins are scattered throughout the lipid double layer.

outside the cells.

 The cell membrane allows certain molecules to pass through freely while others

move through with difficulty and still others do not pass through at all.

 This is selective permeability and the cell membrane is described as semi-permeable.

***Properties of cell membrane*** ***Permeability***

 The cell membrane is semi-permeable.

it allows small molecules that are soluble in lipid to pass through with more ease than water soluble molecules. this is due to the presence of the phospholipids double layer.

***Polarlity***

 The cell membrane has electrical charges across its surface.it has positive charged ions on the outside and negatively charged ions on the inside.this property contributes to electrical impulses sent along nerve cells.

 Sensitivity to changes in temperature and pH  Very high temperatures destroy the semi-permeability nature of the cell membrane because the proteins are denatured by extreme pH values have the same effect on the membrane permeability.

 Physiological processes  Some of the physiological processes include diffusion, osmosis and active transport.

***Diffusion***

 Diffusion is the movement of molecules or ions from a region of high concentration to a

region of low concentration aided by a concentration gradient..

 diffusion continues to occur as long as there is a difference in concentration between two

regions (concentration gradient).

 Stops when an equilibrium is reached i.e., when the concentration of molecules is the same

in both regions.

 Diffusion is a process that occurs inside living organisms as well as the external

environment..

 Does not require energy. ***Factors Affecting Diffusion***

.~ -  An increase in the concentration of molecules at one region results in a steeper concentration gradient which in turn increases the rate of diffusion.  High temperature increases kinetic energy of molecules. They move faster hence resulting in an increase in rate of diffusion, and vice versa.  The smaller the size of molecules or ions, the faster their movement hence higher rate of diffusion.  The denser the molecules or ions diffusing, the slower the rate of diffusion, and vice versa.  The medium through which diffusion occurs also affects diffusion of molecules or ions. For example, diffusion of molecules through gas and liquid media is faster than through a solid medium.  This refers to the thickness or thinness of surface across which diffusion occurs. Rate of diffusion is faster when the distance is small i.e., thin surface.  The larger the surface area to volume ratio, the faster the rate of diffusion. For example, in small organisms such as Amoeba the surface area to volume ratio, is greater hence faster diffusion than in larger organisms.

**Concentration Gradient**

**Temperature**

**Surface Area to Volume Ratio** ***Role of Diffusion in Living Organisms*** **Some processes that depend on diffusion include the following:**

 Gaseous exchange: Movement of gases through respiratory surfaces is by diffusion.  Absorption of materials into cells Cells obtain raw materials and nutrients from the surrounding tissue fluid and blood through diffusion, e.g., glucose needed for respiration diffuses from blood and tissue fluid into cells.

 Excretion: Removal of metabolic waste products like carbon (IV) oxide, and ammonia

out of cells is by diffusion.

 Absorption of the end-products of digestion from the intestines is by diffusion.

***Osmosis***

 Osmosis is the movement of water molecules from a region of high water concentration to a

region of low water concentration through a semi-permeable membrane.

 Osmosis is a special type of diffusion that involves the**movement of water molecules only**

and**not solute molecules.**

 Osmosis takes place in cells across the cell membrane as well as across non-living membranes  e.g. cellophane or visking tubing which are also semi-permeable,  It is purely a physical process.

 ***Factors Affecting Osmosis***

 Osmosis' occurs ylno permeable membrane.  Osmosis occurs when two solutions of unequal solute concentration are separated by a semi-permeable membrane.  **Temperature ,.** High temperatures increase movement of water molecules hence influence osmosis. However, too high temperatures denature proteins in cell membrane and osmosis stops.  Increase in pressure affects movement of water molecules. As pressure increases inside a plant cell, osmosis decreases.

**Size of solute molecules-**

when solute molecules are too large to pass through a semi-

**Concentration Gradient .**

**Pressure**

***Roles of Osmosis in Living Organisms*** The following processes depend on osmosis in living organisms:

 Movement of water into cells from the surrounding tissue fluid and also from cell to

cell.

 Absorption of water from the soil and into the roots of plants.  Support in plants especially herbaceous ones, is provided by turgor pressure, which

results from intake of water by osmosis.

 Absorption of water from the alimentary canal in mammals.  Re-absorption of water in the kidney tubules.  Opening and closing stomata. ***Water Relations in Plant and Animal Cells***  The medium (solution) surrounding cells or organisms is described by the terms

**hypotonic, hypertonic**and**isotonic.**

 A solution whose solute concentration is more than that of the cell sap is said to be

**hypertonic**. A cell placed in such a solution loses water to the surroundings by osmosis.

 A solution whose solute concentration is less than that of the cell sap is said to be

**hypotonic**. A cell placed in such a solution gains water from the surroundings by osmosis.

 A solution which has the same solute concentration as the cell sap is said to be**isotonic**.

When a cell is placed in such a solution there will be no net movement of water either into or out of the cell.

***Osmotic Pressure***

 The term osmotic pressure describes the tendency of the solution with a high solute concentration to draw water into itself when it is separated from distilled water or dilute solution by a semi-permeable membrane.  Osmotic pressure is measured by an osmometer.  When plant cells are placed in distilled water or in a hypotonic solution, the osmotic pressure in the cells is higher than the osmotic pressure of the medium.

 This causes the water to enter the cells by osmosis.  The water collects in the vacuole which increases in size.  As a result the cytoplasm is pushed outwards and it in turn presses the cell membrane next to the cell wall.

 This builds up water pressure (hydrostatic pressure) inside the cell.  When the cell is stretched to the maximum, the cell wall prevents further entry of water into the cell.

 Then the cell is said to be**fully**turgid.  The hydrostatic pressure developed is known as**turgor**pressure.

***Plasmolysis***  When a plant cell is placed in a hypertonic medium, it loses water by osmosis.  The osmotic pressure of the cell is lower than that of the medium.  The vacuole decreases in size and the cytoplasm shrinks as a result of which the cell

membrane loses contact with the cell wall.

 The cell becomes flaccid. The whole process is described as plasmolysis.   Plasmolysis can be reversed by placing the cell in distilled water or hypotonic solution.  However, full plasmolysis may not be reversed if cell stays in that state for long.

**Incipient**plasmolysis is when a cell membrane just begins to lose contact with the cell wall. ***Wilting***  The term wilting describes the drooping of leaves and stems of herbaceous plants after

considerable amounts of water have been lost through transpiration.

 It is observed in hot dry afternoons or in dry weather.  This is when the amount of water lost through transpiration exceeds the amount absorbed

through the roots.

 Individual cells lose turgor and become plasmolysed and the leaves and stems droop.  The condition is corrected at night when absorption of water by the roots continue while

transpiration is absent.

 Eventually, wilting plants may die if the soil water is not increased through rainfall or watering.

***Water Relations in Plants and Animals***

***Haemolysis***

 Haemolysis is the bursting of cell membrane of red blood cells releasing their haemoglobin.   This is because the cell membrane does not resist further entry of water by osmosis after It occurs when red blood cells are placed in distilled water or hypotonic solution.

maximum water intake.

***Crenation***

 Takes place when red blood cells are placed in hypertonic solution.  They lose water by osmosis, shrink and their shape gets distorted.

**Animal cells**have mechanisms that regulate their salt water balance

(osmoregulation) to prevent above processes that lead to death of cells.

 An Amoeba placed in distilled water, i.e. hypotonic solution, removes excess water

using a contractile vacuole.

 The rate of formation of contractile vacuoles increases.

**Active Transport**  Active transport is the movement of solutes such as .glucose, amino acids and

mineral ions;

 From an area of their low concentration to an area of high concentration.   As such it only takes place in living organisms.  The energy needed comes from respiration.  Certain proteins in the cell surface membrane responsible for this movement are

It is movement against a concentration gradient and therefore energy is required.

referred to as**carrier proteins or channel proteins.**

 The shape of each type of carrier protein is specific to the type of substances

conveyed through it. It has been shown that the substance fits into a particular slot on the protein molecule,

 As the protein changes from one form of shape to another the substance is moved

across and energy is expended.

***Factors Affecting Active Transport***

***Availability of oxygen***

 Energy needed for active transport is provided through respiration.  An increase in the amount of oxygen results in a higher rate of respiration.  If a cell is deprived of oxygen active transport stops .

***Temperature***

 Optimum temperature is required for respiration, hence for active transport.  Very high temperatures denature respiratory enzymes.  Very low temperatures inactivate enzymes too and active transport stops.

***Availability of carbohydrates***

 Carbohydrates are the main substrates for respiration.

Increase in amount of carbohydrate results in more energy production during respiration and hence more active transport.

 Lack of carbohydrates causes active transport to stop.

***Metabolic poisons***

 Metabolic poisons e.g. cyanide inhibit respiration and stops active transport due to

lack of energy. ***Role of Active Transport in Living Organisms*** Processes requiring active transport:  Absorption of mineral salts from the soil into plant roots.  Absorption of end products of digestion e.g. glucose and amino acids from the

digestive tract into blood stream.

 Excretion of metabolic products e.g.urea from the cells.  Re-absorption of useful substances and mineral salts back into blood capillaries from

the kidney tubules.

 Sodium-pump mechanism in nerve cells.  Re-absorption of useful materials from tissue fluid into the blood stream.

*Practical Activities* 1.**Experiment to Demonstrate Diffusion**  Various coloured substances such as: dyes, plant extracts and chemicals like

potassium pennanganate are used.

 Potassium manganate (VII) crystals are introduced to the bottom of a beaker filled   Observations are made and the disappearance of the crystals and subsequent

with water using a glass tubing or drinking straw which is then removed.

uniform colouring of water noted.

2.**Experiment to Demonstrate Osmosis Using a Visking Thbing**

 A strip of visking tubing 8-10 cm is cut and tied at one end using strong thread.  About 2 ml of 25% sucrose solution is put inside and the other end tied with thread.  The tubing is washed under running water and then blotted to dry.

It is immersed in a beaker containing distilled water and left for at least one hour or overnight. It will then be observed that the visking tubing has greatly increased in size and has become firm.

 A control experiment can be set up using distilled water inside the visking tubing in

place of sucrose solution.

**3.Experiment to Show Osmosis using Living Tissue**

 Irish potato tubers are peeled and scooped out to make hollow space at the centre.  Sucrose solution is placed inside the hollow, and the potato tuber placed in a beaker or petri-dish with distilled water. A conttrol is set using a boiled potato.  Another one using distilled water inside hollow in place of sugar solution.  The experiment is left for 3 hours to 24 hours.

**4.Experiment to Demonstrate Turgor and Plasmolysis in Onion Epidermal Cells**

 Two strips of onion epidermis are obtained.  One is placed on a slide with distilled water while the other is placed on a slide with

25% sucrose solution and a coverslip placed on top of each.

 The mounted epidermis is observed under low power microscope and then left for 30

minutes.

 After 30 minutes, observations are made again.

The cells in distilled water have greatly enlarged. Cells in 25% sucrose have shrunk. **Nutrition in Plants and Animals**

***Structure of the Leaf***

***External Structure***

The external structure of the leaf consists of a leaf stalk or petiole and a broad leaf blade or lamina. The lamina has a main vein midrib from which smaller veins originate. The outline of the leaf is the margin and the tip forms the apex.

***Internal Structure of the Leaf***

***Epidermis***

 This is the outer layer of cells, normally one cell thick.  It is found in both the upper and lower leaf surfaces.  The cells are arranged end to end.  The epidermis offers protection and maintains the shape of the leaf.  It is covered by a layer of cuticle which reduces evaporation.

***Leaf Mesophyll***

Consists of the palisade layer, next to upper epidermis, and the spongy layer next to the

lower epidermis. ***Palisade Mesophyll Layer***

The cells are elongated and arranged close to each other leaving narrow air spaces. These contain numerous chloroplasts and are the main photosynthetic cells. In most plants, the chloroplast are distributed fairly uniformly throughout the cytoplasm. In certain plants growing in shaded habitats in dim light, most chloroplasts migrate to the upper region of the palisade cells in order to maximise absorption of the limited light available. ***Spongy Mesophyll Layer***

 The cells are spherical in shape.  They are loosely arranged, with large intercellular spaces between them.  The spaces are airfilled and are linked to the stomatal pores.  The spongy mesophyll cells have fewer chloroplasts than the palisade mesophyll

cells. ***Vascular Bundles***  These are made up of the xylem and the phloem tissues.  The xylem transports water and mineral salts to the leaves.  The phloem transports food manufactured in the leaf to the other parts of the plant

and from storage organs to other parts.

***Adaptations of Leaf for Photosynthesis***

 Presence of veins with vascular bundles. Xylem vessels transport water for photosynthesis.  Phloem transports manufactured food from leaves to other parts of the plant.  Leaf lamina is thin to allow for penetration of light over short distance to reach

photosynthetic cells.

 Broad lamina provides a large surface area for absorption of light and carbon (IV)

oxide.

 Transparent cuticle and epidermal layer allow light to penetrate to mesophyll cells.  Palisade cells are close to the upper epidermis for maximum light absorption.  Presence of numerous chloroplasts in palisade mesophyll traps maximum light.  Chloroplast contain chlorophyll that traps light energy.  Spongy mesophyll layer has large intercellular air spaces allowing for gaseous exchange.

 Presence of stomata for efficient gaseous exchange (entry of carbon (IV) oxide into

leaf and exit of oxygen).

 Mosaic arrangement of leaves to ensure no overlapping of leaves hence every leaf is

exposed to light.

***Structure and Function of Chloroplasts***

 Chloroplasts are large organelles (5 um in diameter) found in the cytoplasm of green

plant cells.

 They are visible under the light microscope.  They contain chlorophyll, a green pigment and other carotenoids which are yellow,

orange and red in colour.

 Certain plants have red or purple leaves due to abundance of these other pigments.  Chlorophyll absorbs light energy and transforms it into chemical energy.  The other pigments absorb light but only to pass it onto chlorophyll.

 The wall of chloroplast consists of an outer and an inner membrane.  The two make up the chloroplast envelop.   At intervals, the membranes form stacks of fluid filed sacs known as grana (singular

Inner membrane encloses a system of membranes called lamellae.

granum).

 Chloroplast and other pigments are attached to the grana.

In between the lamellae is a gel-like stroma, that contains starch grains and lipid droplets.

 Enzymes for the dark stage reaction (light independent stage) are embedded in the

stroma.

 Enzymes for the light dependent stage occur in the grana.

***Functions***

 .Absorption of light by chlorophyll and other pigments.  Light stage of photosynthesis occurs on the grana. (transformation of light energy to

chemical energy.)

 Carbon fixation to form carbohydrate takes place in the stroma which has enzymes

for dark stage of photosynthesis.

***Process of Photosynthesis***

 Photosynthesis involves a series of chemical reactions, all of which take place inside

chloroplasts.

 A general equation for photosynthesis is:

Carbon (IV)Oxide+Water light energy---Glucose+Oxygen chlorophyll 6CO2+6H2O light C6H12O+6O2 chlorophyll

 The reaction occurs in two main phases or stages.  The initial state requires light and it is called the light dependent stage or simply light

stage. It takes place on the lamellae surfaces. Its products are used in the dark stage.

   The dark stage does not require light although it occurs in the light and is called light

independent stage.

***Light-Stage***

 Two reactions take place that produce raw materials for the dark stage:  Light energy splits the water molecules into hydrogen and oxygen.  This process is called photolysis.  The hydrogen is taken up by a hydrogen acceptor called Nicotinamide adenine

dinucleotide phosphate (NADP) while oxygen is released as a by-product.

 2H2O(l) light energy4H+O2

 photolysis  Light energy strikes the chlorophyll molecules and sets in motion a series of reactions resulting in the production of a high energy molecule called adenosine triphophate (ATP). ***Dark Stage***

 This stage involves the fixation of carbon i.e. the reduction of carbon (IV) oxide by

addition of hydrogen to form carbohydrate. It uses the products formed during the light stage. Carbon

    The synthesis of carbohydrates does not take place in a simple straight line reaction

*ATP* + Hydrogen --- Carbohydrates (IV) oxide

as shown in the equation above. It involves a series of steps that constitute what is known as the Calvin cycle.

  Carbon (IV) oxide is taken up by a compound described as a carbon (IV) oxide

acceptor.

 This is a 5-carbon compound known as ribulose biphosphate and a six carbon compound is formed which is unstable and splits into two three-carbon compounds.  Hydrogen from the light reaction is added to the three carbon compound using

energy (ATP) from the light reaction.

 The result is a three carbon (triose) sugar, (phosphoglycerate or PGA).  This is the first product of photosynthesis.  Glucose, other sugars as well as starch are made from condensation of the triose sugar molecules.

 The first product is a 3-carbon sugar which condenses to form glucose (6-C sugar).  From glucose, sucrose and eventually starch is made.  Sucrose is the form in which carbohydrate is transported from the leaves to other

parts of the plant.

 Starch is the storage product.  Other substances like oils and proteins are made from sugars.  This involves incorporation of other elements e.g. nitrogen, phosphorus and sulphur.

***Factors Influencing Photosynthesis***

 Certain factors must be provided for before photosynthesis can take place.  The rate or amount of photosynthesis is also influenced by the quantity or quality of

these same factors.

***Carbon(IV) Oxide Concentration***

 Carbon (IV) oxide is one of the raw materials for photosynthesis.  No starch is formed when leaves are enclosed in an atmosphere without carbon (IV)

oxide.

 The concentration of carbon (IV) oxide in the atmosphere remains fairly constant at

about 0.03% by volume. it experimental conditions. Increasing the carbon (IV) oxide concentration up to 0.1 % increases the rate of photosynthesis.

is possible to vary the carbon (IV) oxide concentration under

 Further increase reduces the rate.

***Light Intensity***

 Light supplies the energy for photosynthesis.  Plants kept in the dark do not form starch.  Generally, increase in light intensity up to a certain optimum, increases the rate of

photosynthesis.

 The optimum depends on the habitat of the plant.  Plants that grow in shady places have a lower optimum than those that grow in sunny places.

 ***Water***

 Water is necessary as a raw material for photosynthesis.  The amount of water available greatly affects the rate of photosynthesis.  The more water available, the more the photosynthetic rate, hence amount of food

made.

 Effect of water on photosynthesis can only be inferred from the yield of crops.  It is the main determinant of yield (limiting factor in the tropics).

***Temperature***

 The reactions involved in photosynthesis are catalysed by a series of enzymes.  A suitable temperature is therefore necessary.  The optimum temperature for photosynthesis in most plants is around 30"C.  This depends on the natural habitat of the plant.  Some plants in temperate regions have 20C as their optimum while others in the

tropics have 45C as their optimum temperature.

 The rate of photosynthesis decreases with a decrease in temperature below the

optimum. In most plants, photosynthesis stops when temperatures approach OC although some arctic plant species can photosynthesise at -2C or even -3C.

  Likewise, increase in temperature above the optimum decreases the rate and finally

the reactions stop at temperatures above 40c due to enzyme denaturation.

 However, certain algae that live in hot springs e.g.*Oscilatoria*can photosynthesise at

75C ***Chlorophyll***

 Chlorophyll traps or harnesses the energy from light.  Leaves without chlorophyll do not form starch.

***Chemical Compounds Which Constitute Living Organisms***

 All matter is made up of chemical elements, each of which exists in the form of

smaller units called atoms.

 Some of the elements occur in large amounts in living things.  These include carbon, oxygen, hydrogen, nitrogen, sulphur and phosphorus.  Elements combine together to form compounds.  Some of these compounds are organic.  Organic compounds contain atoms of carbon combined with hydrogen and they are

usually complex.

 Other compounds are inorganic.  Most inorganic compounds do not contain carbon and hydrogen and they are usually

less complex.

 Cells contain hundreds of different classes of organic compounds.  However, there are four classes of organic compounds found in all cells.  These are:*carbohydrates, lipids, proteins and nucleic acids.*

***Carbohydrates***

 Carbohydrates are compounds of carbon, hydrogen and oxygen.  Hydrogen and oxygen occur in the ratio of 2: 1 as in water.  Carbohydrates are classified into three main groups: monosaccharides, disaccharides

and polysaccharides.

***Monosaccharides***

 These are simple sugars.  The carbon atoms in these sugars form a chain to which hydrogen and oxygen atoms

are attached.

 Monosaccharides are classified according to the number of carbon atoms they

possess.

 The most common monosaccharides are:

 Glucose - found free in fruits and vegetables.   Galactose - found combined in milk sugar.

Fructose - found free in fruits and in bee honey.

 The general formula for these monosaccharides is (CH2O)n where n is 6.  They have the same number of carbon, hydrogen and oxygen molecules i.e. C6H12O6*.*

***Properties of Monosaccharides***  They are soluble in water.  They are crystallisable.  They are sweet.  The are all reducing sugars.  This is because they reduce blue copper (II) sulphate solution when heated to copper

oxide which is red in colour and insoluble.

***Functions of Monosaccharides***

 They are oxidised in the cells to produce energy during respiration.  Formation of important biological molecules e.g. deoxyribonucleic acid (DNA) and

ribonucleic acid (RNA).

 Some monosaccharides are important metabolic intermediates e.g. in

photosynthesis and in respiration.

 Monosaccharides are the units from which other more complex sugars are formed

through condensation.

***Disaccharides***

 These contain two monosaccharide units.  The chemical process through which a large molecule (e.g. a disaccharide) is formed

from smaller molecules is called condensation and it involves loss of water.

Common examples of disaccharides include sucrose, maltose and lactose.

*Monosaccharide units Disaccharides* Glucose+fructose Glucose+glucose Glucose+galactose Sucrose(cane sugar) Maltose(malt sugar) Lactose(milk sugar)  Disaccharides are broken into their monosaccharide units by heating with dilute

hydrochloric acid.

 This is known as hydrolysis and involves addition of water molecules.  The same process takes place inside cells through enzymes.

Sucrose+water_--hydrolysis-----------------glucose+fructose

***Properties of Disaccharides***

 Sweet tasting.  Soluble in water.  Crystallisable.  Maltose and lactose are reducing sugars while sucrose is non-reducing sugar.  Sucrose is the form in which carbohydrate is transported in plants:  This is because it is soluble andjchernically stable.  Sucrose is a storage carbohydrate in some plants e.g. sugar-cane and sugar-beet.  Disaccharides are hydrolysed to produce monosaccharide units which are readily

metabolised by cell to provide energy.

***Polysaccharides***

If many monosaccharides are polysaccharide is formed.

joined together through condensation, a

 Polysaccharides may consist of hundreds or even thousands of monosaccharide

units.

 Examples of polysaccharides:  Starch - storage material in plants.  Glycogen is a storage carbohydrate in animals like starch, but has longer

chains. Inulin - a storage carbohydrate in some plants e.g. Dahlia. Cellulose - structural carbohydrate in plants. Chitin - forms exoskeleton in arthropods.

***Importance and Functions of Polysaccharides***

 They are storage carbohydrates - starch in plants glycogen in animals.  They are hydrolysed to their contituent monosaccharide units and used for

respiration. .

 They form structural material e.g. cellulose makes cell walls.  Cellulose has wide commercial uses e.g. Fibre in cloth industry. Cellulose is used to make paper.

Carbohydrates combine with other molecules to form compounds in living organisms. Examples are:

important structural

Pectins: Combine with calcium ions to form calcium pectate. Chitin: Combine with (NH) group. Makes the exoskeleton of arthropods, and walls

of fungi. ***Lipids***

These are fats and oils. Fats are solid at room temperature while oils are liquid. They are made up of carbon, oxygen and hydrogen atoms. The structural units of lipids are fatty acids and glycerol. Fatty acids are made up of hydrocarbon chain molecules with a carboxyl group (-COOH) at one end. In the synthesis of a lipid, three fatty acid molecules combine with one glycerol molecule to form a triglyceride. Three molecules of water are lost in the process. This is a condensation reaction and water is given off. Lipids are hydrolysed e.g. during digestion to fatty acids and glycerol, water is added.

*condensation* -

Glycerol + 3 Fatty*hydrolysis*Lipid + Water acids ***Properties***

  Fats are insoluble in water but dissolve in organic solvents e.g. in alcohols. They are chemically inactive, hence used as food storage compounds.

***Functions of Lipids***

***Structural materials***- as structural material they make up the cell membrane. ***Source of energy***- they are energy rich molecules.

One molecule of lipid provides more energy than a carbohydrate molecule.

***Storage compound***- They are stored as food reserves in plants.

In animals e.g. mammals, all excess food taken is converted to fats which are stored in adipose tissue, and around internal organs such as the heart and kidneys. ***Insulation***- They provide insulation in animals living in cold climates. A lot of fat is stored under the skin e.g. blubber in seals.

***Protection***- Complex lipids e.g. wax on leaf surfaces protects the plant against water-

loss and overheating.

**Fats stored around some internal organs acts as shock absorbers**, thus protecting

the organs.

***Source of Metabolic Water*-:-**lipids when oxidised produce metabolic water which

supplements water requirements in the body. Desert animals e.g. the camel accumulate large quantities of fat in the hump which when oxidised releases metabolic water.

***Proteins***

 Proteins are the most abundant organic compounds in cells and constitute 50% of

total dry weight.

 Proteins are compounds which are made up of carbon, hydrogen, nitrogen, oxygen

and sometimes sulphur and phosphorus.

 The structural units of proteins are amino acids.  The nature of a protein is determined by the types of amino acids it is made of.  There are about 20 common amino acids that make up proteins. ***Essential and Non-Essential Amino Acids***

 Essential amino acids are those which cannot be synthesised in the body of an

organism and must therefore be provided in the diet.  There are ten amino acids which are essential for humans.  These are valine, leucine, phenylalanine, lysine, tryptophan, isoleucine, methionine,

threonine, histidine and arginine.

 Non-essential amino acids are those which the body can synthesise and therefore

need not be available in the diet.

 There are ten of them.  These are glycine, alanine, glutamic acid, aspartic acid, serine, tyrosine, proline,

glutamine, arginine and cysteine.

 Proteins are essential in the diet because they are not stored in the body.  Excess amino acids are deaminated.

***Formation of Proteins***

 Proteins are made up of many amino acid units joined together through peptide

bonds.

 When two amino acids are joined together a dipeptide is formed.  The chemical process involved is called condensation and a molecule of water is ***Functions of Proteins***

***As structural materials proteins*-**

 Are the basic building structures of protoplasms.  Proteins in conjunction with lipid form the cell membrane.

 Examples of structural proteins include:

 Keratin (in hair, nails, hoofs, feathers and wool)  Silk in spider's web.  Elastin forms ligaments that join bones to each other.

***Protective proteins*.**

 Antibodies that protect the body against foreign antigens.  Fribrogen and thrombin are involved in clot formation, preventing entry of

micro-organisms when blood vessel is cut. ***As functional chemical compounds*.**

 Examples are hormones and enzymes that act as regulators in the body.  Respiratory pigments.  Examples are haemoglobin that transports oxygen in the blood and

myoglobin that stores up oxygen in muscles.

 Contractile proteins - make up muscles, i.e. myosin and actin.  Proteins combine with other chemical groups to form important substances

e.g. mucin in saliva.

***Source of energy*.**

 Proteins are a source of energy in extreme conditions when carbohydrates

and fats are not available e.g. in starvation.

***Enzymes***

 Enzymes are biological catalysts that increase the rate of chemical reaction in the

body.

 They are all produced inside cells.  Some are intracellular and they catalyse reactions within the cells .  Others are extracellular and are secreted out of the cells where they work. e.g.

digestive enzymes. ***Properties of Enzymes***

 Enzymes are protein in nature.  Enzymes are specific to the type of reaction they catalyse.  This is referred to as substrate specificity.  Enzymes work in very small amounts.  They remain unchanged after the reaction.  They catalyse reversible reactions.  They work very fast (high turnover numbers) e.g. the enzyme catalase works on 600

thousand molecules of hydrogen peroxide in one second. ***Naming of enzymes***

Enzymes are named by adding the suffix -ase to: *Name of substrate that they work on e.g.*

 carbohydrates - carbohydrases e.g.sucrase.  Starch (amylose) - amylase  Protein - proteinase (protease)  Lipids -lipases

*Type of chemical reaction catalised e.g.*

 Oxidation - oxidase  Reduction - reductase  Hydrolysis - hydrolase

***Factors Affecting Enzyme Action*** ***Temperature***

 Enzymes are sensitive to temperature changes.  Generally, the rate of an enzymecontrolled reaction doubles with every 10OC increase

in temperature.

 However, temperatures above 40C do not favour enzyme reaction.  This is because enzymes are denatured by high temperatures.

***pH***

 Every enzyme has a particular pH range over which it works best.  Some enzymes work best in acidic media while others function better in alkaline

media.

 Many enzymes function well under neutral conditions.

***Enzyme Concentration***

 Under conditions where the substrate is in excess, the rate of an enzyme-controlled

reaction increases as the enzyme concentration is increased. ***Substrate Concentration***

 If the concentration of the substrate is increased while that of the enzyme remains constant, the rate of the reaction will increase for sometime and then become constant.

 Any further increase in substrate concentration will not result in corresponding

increase in the rate of the reaction.

***Enzyme Inhibitors***

 These are substances that either compete with substrates for enzyme active sites or

combine with enzymes and hence they inhibit the enzyme reaction.

 e.g. certain drugs, cyanide and nerve gas.

***Co-factors***

 Most enzymes require the presence of other compounds known as co-factors which

are non-proteins.

 There are three groups of co-factors.   Complex organic molecules known as prosthetic groups are attached to the enzyme

Inorganic ions - e.g. iron, magnesium, copper and zinc.  e.g. flavin adenine dinucleotide (FAD) derived from vitamin B2 (riboflavin).  Co-enzymes e.g. coenzyme A is involved in respiration.  All co-enzymes are derived from vitamins. ***Nutrition in Animals=**Heterotrophism* ***Meaning and Types of Heterotrophism***

 This is a mode of nutrition whereby organisms feed on complex organic matter from

other plants or animals.  All animals are heterotrophs.  Their mode of feeding is also said to be holozoic to distinguish it from other special

types of heterotrophic nutrition namely:

 saprophytism  parasitism.

 Saprophytism/saprotrophysim- occurs in most fungi and some forms of bacteria.  Saprophytes feed on dead organic matter and cause its decomposition or decay.  Parasitism is a mode of feeding whereby one organism called the parasite feeds on or

lives in another organism called the host and harms it. ***Modes of Feeding in Animals***

 Animals have developed various structures to capture and ingest food.  The type of structures present depend on the method of feeding and the type of

food.

**Carnivorous**animals feed on whole animals or portions of their flesh. **Herbiverous**animals feed on plant material. ***Omnivorous***animals feed on both plants and animal materials.

***Feeding in Mammals***

 The jaws and teeth of mammals are modified according to the type of food eaten.  Mammals have different kinds of teeth.  Each type of teeth has a particular role to play in the feeding process.

***Feeding in Mammals***

 The jaws and teeth of mammals are modified according to the type of food eaten.  Mammals have different kinds of teeth.  Each type of teeth has a particular role to play in the feeding process.  This condition is described as heterodont.  The teeth of reptiles and amphibians are all similar in shape and carry out the same

function.

 They are said to be homodont.

**Types of Mammalian Teeth**

 Mammals have four kinds of teeth.  The incisors are found at the front of the jaw.  They are sharp-edged and are used for biting.  The canines are located at the sides of the jaw.  They are pointed and are used for tearing and piercing.  The premolars are next to the canines and the molars are at the back of the jaw.  Both premolars and molars are used for crushing and grinding.  Teeth are replaced only once in a lifetime.  The first set is the milk or deciduous teeth.  These are replaced by the second set or the permanent teeth.

 Dentition refers to the type of teeth, the number and their arrangement in the jaw.  A dental formula shows the type and number of teeth in each half of the jaw.  The number of teeth in half of the upper jaw is represented above a line and those on

the lower jaw below the line.

 The first letter of each type of teeth is used in the formula i.e. i =incisors, c = canines,

pm = premolars and m = molars.

 The total number is obtained by multiplying by two (for the two halves of each jaw).

***Adaptation of Teeth to Feeding***

In general, incisors are for cutting, canines for tearing while premolars and molars are for grinding.

 However, specific modifications are observed in different mammals as an adaptation

to the type of food they eat.

 Teeth of Herbivores   The enamel coating is thicker in front than at the back so that as the tooth wears out,

Incisors are long and flat with a sharp chisellike edge for cutting.

a sharp edge is maintained.  Canines are reduced or absent.   The diastema allows the tongue to hold food and push it to the grinding teeth at the

back of the mouth. ***Premolars and molars:***

 These are transversely ridged.  The ridges on the upper teeth fit into grooves on the lower ones.  This gives a sideways grinding surface.  The teeth of herbivores have open roots i.e., wide opening into the pulp cavity.  This ensures a continued adequate supply of food and oxygen to the tooth.

In some herbivores, such as rabbits and elephants, the incisors continue to grow throughout life. ***Teeth of Carnivores***

  They are well suited for grasping food and holding prey.  Canines are long, pointed and curved.  They are used for piercing and tearing flesh as well as for attack and defence. ***Premolars and molars**:*In general, they are long and longitudinally ridged to

Incisors are reduced in size and pointed.

increase surface area for crushing .

***Carnassial Teeth**:*These are the last premolars on the upper jaw and the first

molars on the lower one.

 They are enlarged for cutting flesh.  They act as a pair of shears.  They also crush bones.  The teeth of carnivores have closed roots i.e., only a very small opening of the pulp  The bacteria then infect the pulp cavity and the whole tooth decays.

***Treatment***

 Treatment depends on the extent of the dental caries:  Extraction of Tooth.  Filling - this involves replacing the dentine with amalgam, a mixture of hard

elements e.g. silver and tin.

 Root Canal Treatment - This involves surgery and reconstruction.  It saves severely damaged teeth.  The nerves in the root canal are surgically severed.  The tooth is cleaned and filled up with amalgam. ***Periodontal Diseases***

 These are diseases of the gum.  The gum becomes inflamed, and starts bleeding.  Progression of the disease leads to infection of the fibres in the periodontal

membranes and the tooth becomes loose.

 This condition is known as pyorrhoea.  The diseases are caused by poor cleaning of the teeth.  The accumulation of food particles leading to formation of plaque, lack of adequate

vitamin A and C in the diet.

***Treatment***  Nutrition - by taking adequate balanced diet rich in vitamins A and C.  Antibiotics are used to kill bacteria.  Anti-inflamatory drugs are given.  Antiseptic is prescribed to use in cleaning the mouth daily to prevent further

proliferation of bacteria.

 The plaque is removed-drilled away - a procedure known as scaling.

***Care of Teeth*** In order to maintain healthy teeth the following points should be observed:

 A proper diet that includes calcium and vitamins, particularly vitamin D is essential.  The diet should also contain very small quantities of fluorine to strengthen the

enamel.  Large quantities of fluorine are harmful.  The enamel becomes brown, a condition known as dental flourosis.  Chewing of hard fibrous foods like carrots and sugar cane to strengthen and cleanse

the teeth.

 Proper use of teeth e.g. not using teeth to open bottles and cut thread.  Regular and thorough brushing of teeth after meals.  Dental floss can be used to clean between the teeth.  Not eating sweets and sugary foods between meals.  Regular visits to the dentist for checkup.  Washing the mouth with strong salt solution or with any other mouth wash with

antiseptic properties.

***Digestive System and Digestion in Humans***

 Organs that are involved with feeding in humans constitute the digestive system. ***Digestive System and Associated Glands***

 Human digestive system starts at the mouth and ends at the anus.  This is the alimentary canal.  Digestion takes place inside the lumen of the alimentary canal.  The epithelial wall that faces the lumen has mucus glands (goblet cells).

 These secrete mucus that lubricate food and prevent the wall from being digested by

digestive enzymes.

 Present at specific regions are glands that secrete digestive enzymes.  The liver and pancreas are organs that are closely associated with the alimentary

canal.

 Their secretions get into the lumen and assist in digestions.

***Digestive system consists of:***

 Mouth.  Oesophagus.  Stomach.  Small intestines - consist of duodenum, the first part next to the stomach, ileum - the last part that ends up in a vestigial caecum and appendix which are nonfunctional.

 Large intestines consist of: colon and rectum that ends in the anus. ***Ingestion, Digestion and Absorption***

 Feeding in humans involves the following processes:  *Digestion:*This is the mechanical and chemical breakdown of the food into simpler,

*Ingestion*: This is the introduction of the food into the mouth.

soluble and absorbable units.

*Absorption:*Taking into blood the digested products. *Assimilation:*Use of food in body cells.  Mechanical breakdown of the food takes place with the help of the teeth.  Chemical digestion involves enzymes.

***Digestion in the Mouth***

  Food is mixed with saliva and is broken into smaller particles by the action of teeth.  Saliva contains the enzyme amylase.

In the mouth, both mechanical and chemical digestion takes place.

It also contains water and mucus which lubricate and soften food in order to make swallowing easy.

 Saliva is slightly alkaline and thus provides a suitable pH for amylase to act on cooked

starch, changing it to maltose.

 The food is then swallowed in the form of semisolid balls known as boluses.  Each bolus moves down the oesophagus by a process known as peristalsis.  Circular and longitudinal muscles along the wall of the alimentary canal contract and

In the stomach, the food is mixed with gastric juice secreted by gastric glands in the stomach wall.

 Gastric juice contains pepsin, rennin and hydrochloric acid.  The acid provides a low pH of 1.5-2.0 suitable for the action of pepsin.  Pepsin breaks down protein into peptides.  Rennin coagulates the milk protein casein.  The stomach wall has strong circular and longitudinal muscles whose contraction

mixes the food with digestive juices in the stomach. ***Digestion in the Duodenum***

  Bile contains bile salts and bile pigments.  The salts emulsify fats, thus providing a large surface area for action of lipase.  Pancreatic juice contains three enzymes:

In the duodenum the food is mixed with bile and pancreatic juice.

 Trypsin which breaks down proteins into peptides and amino acids,  Amylase which breaks down starch into maltose, and  Lipase which breaks down lipids into fatty acids and glycerol.

 These enzymes act best in an alkaline medium which is provided for by the bile. ***Digestion in ileum***

 Epithelial cells in ileum secrete intestinal juice, also known as succus entericus.  This contains enzymes which complete the digestion of protein into amino acids,

carbohydrates into monosaccharides and lipids into fatty acids and glycerol.

***Absorption***

 This is the diffusion of the products of digestion into the blood of the animal.

It takes place mainly in the small intestines though alcohol and some glucose are absorbed in the stomach.

***The ileum is adapted for absorption in the following ways:***

  The coiling ensures that food moves along slowly to allow time for its digestion and

It is highly coiled.

absorption. It is long to provide a large surface area for absorption.

  The epithelium has many finger-like projections called villi (singular villus).

 They greatly increase the surface area for absorption.  Villi have microvilli that further increase the surface area for absorption.  The wall of villi has thin epithelial lining to facilitate fast diffusion of products of

digestion.

 Has numerous blood vessels for transport of the end products of digestion.  Has lacteal vessels; for absorption of fatty acids and glycerol and transport of lipids.  First they are carried to the liver through the hepatic portal vein, then taken to all

organs via circulatory system.

***Absorption of Fatty Acids and Glycerol***

 Fatty acids and glycerol diffuse through the epithelial cells of villi and into the lacteal.  When inside the villi epithelial cells, the fatty acids combine with glycerol to make

tiny fat droplets which give the lacteal a milky appearance.

 The lacteals join the main lymph vessel that empties its contents into the

bloodstream in the thoracic region.

 Once inside the blood, the lipid droplets are hydrolysed to fatty acids and glycerol.

***Absorption of Vitamins and Mineral Salts***

 Vitamins and mineral salts are absorbed into the blood capillaries in' the villi. Water is

mainly absorbed in the colon.

 As a result the undigested food is in a semi-solid form (faeces) when it reaches the

rectum.

***Egestion*:**This is removal of undigested or indigestible material from the body.

Faeces are temporarily stored in the rectum then voided through the anus. Opening of the anus is controlled by sphincter muscles

***Assimilation:***This is the incorporation of the food into the cells where it is used for

various chemical processes. ***Carbohydrates***

 used to provide energy for the body.  Excess glucose is converted to glycogen and stored in the liver and muscles.  Some of the excess carbohydrates are also converted into fat in the liver and stored in the adipose tissue' (fat storage tissue), in the mesenteries and in the connective tissue under the skin, around the heart and other internal organs.

***Proteins***

 Amino acids are used to build new cells and repair worn out ones.  They are also used for the synthesis of protein compounds.  Excess amino acids are de-aminated in the liver.  Urea is formed from the nitrogen part.  The remaining carbohydrate portion is used for energy or it is converted to glycogen

or fat and stored.

***Lipids***

 Fats are primarily stored in the fat storage tissues.  When carbohydrates intake is low in the body, fats are oxidised to provide energy.  They are also used as structural materials e.g. phospholipids in cell membrane. They

act as cushion, protecting delicate organs like the heart.

 Stored fats under the skin act as heat insulators. Pepsin Protein

Trypsin Protein

Chymotrypsi n Peptones, casein

8.8 Starch glycogen Amylase

Lipase Lipids Glucose if food stays longer in mouth. Maltose

Not an enzyme but hydrolyses the nuclear proteins. 1. Kills micro-organisms. 2. Provides acidic medium. 3. Activates enzyme pepsinogen and protennin. precursors,

Hydrochloric Nucleo- acid Nucleic + protein acid proteins

Curd coagulated abundant in infants secreted milk (casein)

Peptones Secreted as pepsinogen

Secreted as trypsinogen activated by enterokinase to trypsin

Amino acids Secreted as chymotrypsin

activated to trypsin.

Maltose Maltose Fatty acids PH in duodenum lowered to and glycerol

Sodium bicarbonate Peptidases (erepsin) Invertase made of sucrase

Provides alkaline conditions

Erepsin contains a mixture of peptidases Amino acids Peptides

Fructose +

glucose

-.

Ileum (succus entericus) Galactose + glucose 8.3 Lactase Lactose

 Maltase Maltose Glucose

Fatty acids and glycerol

Activates trypsinogen to trypsin. Enterokinase ***Vitamins, sources, uses and the deficiency disease resulting from their absence in diet***

*Name of Vitamin Sources* *Uses in body* *Deficiency disease(s)/Disorder*I

Liver, egg-yolk, Synthesis of rhodopsin, Hardening of cornea of the eye (xerophthalmia), poor night resistance to diseases of skin and vision; gut is reduced. spinach epithelium

Formation f the enzyme Yeast, whole co- carboxylase important in slowing of heartbeat and grain, liver, kidney, beans, conversion of pyruvic meat, spinach respiration. acid Formation of Whole grain, f1avoproteins eggs, milk, that form groundnuts, liver, cheese, yeast enzymes and for dehydrogenase respiration.

Beriberi - swelling of the feet;

B, (Thiamine)

gastro intestinal disorder.

Liver, kidneys, Makes co-enzyme 1 and 2

B3 (Nicotinic acid) milk, yeast whole grain. eggs, (NAD & NAD.P) It is also Pellagra - inflammation of co-enzyme A needed in respiration. cell nervous disorders leading to tongue; paralysis. B, (Pantothenic

In most natural Forms parts of co- foods enzyme A. acid)

Eggs, kidneys, B6 (Pryidoxine) Makes a co-enzyme for

Irritability, depression, dermatitis. whole grain,

water soluble vegetables. amino acids metabolism. *4* *4*

*N* *U* *T* Nervour transmission *R* *I* *T* *I* *O* *N*

*K* *C* Potassium *S* *E*

 Milk, eggs, liver,

In intracellular body fluids as a transmission. impulse Present in tissue fluid. Maintains water balance essential for

green vegetables, bananas. buffer and for nerve

*R* Chloride *E* *V* *I* *S* Magnesium *I* *O* *N* Iodine

Table salt, sea foods. digestion. Constituent of

*I* *N*

hydrochloric acid. Also needed as a co-factor in respirator enzymes. Muscle contraction. Constituent of the hormone In young animals leads to thyroxine that controls cretinism. Simple goitre adults. metabolism. in body

Green vegetables.

*P* *L* *A* *N* *T* *S*

Iodised table salt and sea food.

*B* *I* Manganese *O* *L* *O* Iron *G* *Y*

Eggs, milk, fish. Activates certain enzymes. *A* *N* Anaemia. *D*

A constituent of cytochromes haemoglobin and myoblobin. A constituent of some proteins; needed in synthesis of certain enzymes and phospholipids In intracellular body fluids in cell membranes. as a transmission. impulse Catalyses use of iron, a Present in tissue fluid. constituent of cytochrome Maintains water balance essential for oxidase (an enzyme) digestion. Constituent of Influences the use of hydrochloric acid. copper and iron (found in Vitamin ~2)' amounts. Also needed as a co-factor Needed for proper growth in respirator enzymes. Muscle of hair, influences working of contraction. insulin. Constituent of the hormone In young animals leads Needed in small Pernicious anaemia. Strengthening of enamel to thyroxine that controls cretinism. Simple goitre amounts. adults. metabolism. in body

Sulphur Protein foods

 Milk, eggs, liver,

green vegetables, bananas. buffer and for nerve Potassium

Copper

Chloride

Cobalt

Fruits and Green vegetables. Synthesised in vegetables. Seeds of the alimentary Used in the manufacture cucubitaceae. Iodised table salt and of red blood cells, synthesis canal by bacteria, meat, Water, fruits and sea of milk, eggs and RNA food. fish. Liver, greet vegetable leaves, lean meat, grains, milk. whole

*A* *N* *I* Nervour transmission *M* *A* interfered with. *L* Needed in very small *S*

*p*

amounts. *4* *3* Needed in very small

Table salt, sea foods.

Needed in very small Magnesium Zinc amounts.

BJ2 (cyanocobalamin Fluorine Iodine e)

Synthesis of nucleoproteins and erythrocytes Anaemia. (most common in pregnant women)

Folic acid*I*vit M Liver, green Eggs, milk, fish. Manganese vegetables (water ~" A constituent of Liver, greet soluble) Synthesised by " T cytochromes vegetable haemoglobin and leaves, lean meat, Iron intestinal Proteins synthesis, used Vitamin H Biotin grains, milk. myoblobin. whole bacteria, liver, transamination and (Water in A constituent of some carboxylation kidney, eggs. Soluble)

Activates certain enzymes. vegetables.

Very small amounts needed, Plant seeds Activates enzyme system in

mucleic acid metabolism. excess is dangerous.

Involved in use of glucose. Needed in small amounts.

***Roughage***

 Roughage is dietary fibre and it consists mainly of cellulose.  It adds bulk to the food and provides grip for the gut muscles to enhance peristalsis.  Roughage does not provide any nutritional value because humans and all animals not

produce cellulase enzyme to digest cellulose. In herbivores symbiotic bacteria in the gut produce cellulase that digests cellulose. ***Factors Determining Energy Requirements in Humans***

***Age:***Infants, for instance, need a greater proportion of protein than adults. ***Sex**:*males generally require more carbohydrates than females.  The requirements of specific nutrients for females depends on the stage of

development in the life cycle.

**Adolescent girls**require more iron in their diet; expectant and nursing mothers

require a lot of proteins and mineral salts.

***State of Health*:**A sick individual requires more of certain nutrients e.g. proteins,

than a healthy one.

***Occupation:***An office worker needs less nutrients than a manual worker. ***THE END*** ***Practical Activities***

 Experiments to show that Carbon (IV) Oxide is necessary for Photosynthesis

 Experiment to Show Effect of Light on Photosynthesis

 Experiment to Show the Effect of Chlorophyll on Photosynthesis

 Experiment To Observe Stomata Distribution in Different Leaves

 Test for Reducing Sugar

 Test for non-reducing sugar

 Test for Lipids; (a) Grease Spot Test

 (b) Emulsion Test    Dissection of a Rabbit to show the Digestive System

Test for Proteins -Biuret Test Experiment To Investigate Presence of Enzyme in Living Tissue