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- # Bacteria-2033Images-33Types-dataset
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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- Microscopy image dataset of 33 bacteria species for machine learning and deep learning research.
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- ## 🧫 33-Class Bacterial Microscopy Image Dataset (2033 Images)
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- This dataset contains 2,033 high-resolution RGB images of **33 bacterial species**, collected from clinical samples (blood, urine, skin), Gram-stained, and annotated by laboratory experts.
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- ![bacteria](https://github.com/user-attachments/assets/7a3044b8-8f1d-427b-8e32-169414c42652)
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- ---
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- ## Dataset Facts
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- | Property | Value |
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  |---|---|
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- | Total images | 2,033 |
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- | Number of classes | 33 bacterial species |
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- | Image type | High-resolution RGB |
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- | Sample source | Clinical (blood, urine, skin) |
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- | Staining method | Gram stain |
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- | Annotation | Expert laboratory annotation |
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- | Download size | ~3.4 GB (ZIP) |
 
 
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  | License | MIT |
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- ---
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- ## 📦 Download the Dataset
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- 🔗 [Click here to download the dataset (3.4 GB, Google Drive)](https://drive.google.com/file/d/1aR7Dz11wKV3t7awnnnO32UE_37MYF6wX/view?usp=sharing)
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- ---
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- ## 📚 Key Publications
 
 
 
 
 
 
 
 
 
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- 1. Jamshidi, M. B., Sargolzaei, S., Foorginezhad, S., & Moztarzadeh, O. (2023). Metaverse and microorganism digital twins: A deep transfer learning approach. *Applied Soft Computing*, 147, 110798.
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- https://www.sciencedirect.com/science/article/pii/S1568494623008165
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- 2. Jamshidi, M.B., Hoang, D.T., Nguyen, D.N., Niyato, D. and Warkiani, M.E. (2025). Revolutionizing biological digital twins: Integrating internet of bio-nano things, convolutional neural networks, and federated learning. *Computers in Biology and Medicine*, 189, 109970.
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- https://www.sciencedirect.com/science/article/pii/S001048252500321X
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- 3. Jamshidi, M., Hoang, D.T. and Nguyen, D.N. (2024). CNN-FL for Biotechnology Industry Empowered by Internet-of-BioNano Things and Digital Twins. *IEEE Internet of Things Magazine*, 7(5), 54–63.
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- https://ieeexplore.ieee.org/abstract/document/10643983
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- ---
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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- ## 📥 Citation
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- If you use this dataset in any research paper, project, book, thesis, or any other scholarly or commercial work, please cite it as follows:
 
 
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  ```bibtex
 
 
 
 
 
 
 
 
 
 
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  @article{jamshidi2023metaverse,
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  title={Metaverse and microorganism digital twins: A deep transfer learning approach},
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- author={Jamshidi, Mohammad Behdad and Sargolzaei, Saleh and Foorginezhad, Salimeh and Moztarzadeh, Omid},
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  journal={Applied Soft Computing},
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  volume={147},
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  pages={110798},
@@ -60,40 +147,13 @@ If you use this dataset in any research paper, project, book, thesis, or any oth
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  }
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  ```
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- ---
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-
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- ## 🤖 Working with the Dataset (ML/AI)
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- - **Download**: Download the ZIP from Google Drive (link above) and extract locally. There is no programmatic API.
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- - **Directory layout**: One folder per class label (33 folders), each containing the class images.
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- - **Recommended frameworks**: PyTorch (`torchvision.datasets.ImageFolder`) or TensorFlow/Keras (`image_dataset_from_directory`) both work naturally with this per-class folder layout.
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- - **Class imbalance**: 2,033 images across 33 classes averages ~62 images per class. Expect imbalance; use weighted loss or data augmentation accordingly.
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- - **Preprocessing**: Standard ImageNet normalization is a reasonable starting point for transfer learning on these Gram-stained RGB images.
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- - **Train/val/test splits**: No official split is defined. Stratified splitting to preserve class proportions is recommended, consistent with published papers.
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-
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- ---
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-
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- ## 🗂️ Repository Structure
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-
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- ```
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- Bacteria-2033Images-33Types-dataset/
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- ├── README.md # Dataset description, download link, citation info
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- └── LICENSE # MIT License
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- ```
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-
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- The image data (3.4 GB) is distributed via Google Drive, not stored in this repository.
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-
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- ---
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-
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- ## 🛠️ Development Workflow (for Contributors)
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-
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- - Work on a feature branch, never directly on `main`.
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- - All changes in this repository are documentation-only (no code or binary data).
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- - Do not commit image data or large binary files.
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- - Push the feature branch and open a pull request into `main` when ready.
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-
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- ---
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  ## Maintainer
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- Mohammad Behdad Jamshidi ([@MBJamshidi](https://github.com/MBJamshidi))
 
 
 
 
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+ ---
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+ license: mit
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+ pretty_name: Bacteria 2033 Images 33 Types Data
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+ task_categories:
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+ - image-classification
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+ language:
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+ - en
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+ tags:
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+ - bacteria
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+ - microscopy
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+ - microbiology
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+ - biomedical
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+ - medical-imaging
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+ - gram-stain
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+ - computer-vision
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+ - image-classification
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+ - transfer-learning
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+ - digital-twin
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+ size_categories:
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+ - 1K<n<10K
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+ viewer: false
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+ ---
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+ # Bacteria 2033 Images 33 Types Data
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+ ## Dataset Summary
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+ **Bacteria 2033 Images 33 Types Data** is a microscopy image dataset for bacterial image classification, transfer learning, and biomedical computer vision research. It contains **2,033 RGB microscopy images** representing **33 bacterial types**, with images organized by class label.
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+ The dataset is intended for research on microorganism recognition, clinical microscopy image analysis, deep transfer learning, biological digital twins, and related machine learning workflows.
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+ ![Bacteria microscopy examples](https://github.com/user-attachments/assets/7a3044b8-8f1d-427b-8e32-169414c42652)
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+ ## Dataset Details
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+ | Field | Description |
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  |---|---|
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+ | Dataset type | Image classification |
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+ | Modality | RGB microscopy images |
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+ | Number of images | 2,033 |
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+ | Number of classes | 33 bacterial types |
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+ | Annotation | Class labels assigned by laboratory experts |
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+ | Staining | Gram-stained microscopy images |
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+ | Source material | Clinical sample imagery, including blood, urine, and skin samples |
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+ | Repository data format | One folder per bacterial class |
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+ | Download size | Approximately 3.4 GB |
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  | License | MIT |
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+ ## Access and Download
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+ The image archive is distributed through Google Drive:
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+ [Download the dataset archive](https://drive.google.com/file/d/1aR7Dz11wKV3t7awnnnO32UE_37MYF6wX/view?usp=sharing)
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+ After downloading, extract the archive locally. The expected layout is:
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+ ```text
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+ Bacteria-2033Images-33Types-dataset/
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+ |-- class_1/
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+ | |-- image_001.jpg
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+ | `-- ...
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+ |-- class_2/
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+ | |-- image_001.jpg
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+ | `-- ...
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+ `-- ...
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+ ```
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+ This folder-per-class structure is compatible with common image-classification loaders such as:
 
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+ - PyTorch: `torchvision.datasets.ImageFolder`
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+ - TensorFlow/Keras: `tf.keras.utils.image_dataset_from_directory`
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+ ## Intended Uses
 
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+ This dataset may be used for:
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+
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+ - Bacterial species or bacterial type image classification
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+ - Deep transfer learning and feature extraction experiments
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+ - Microorganism digital twin research
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+ - Microscopy image analysis
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+ - Biomedical computer vision benchmarking
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+ - Federated learning and distributed learning experiments where local image folders are used as client data
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+
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+ ## Out-of-Scope Uses
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+
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+ This dataset is not intended to be used as a standalone clinical diagnostic system. Models trained on this dataset should not be deployed for clinical decision-making without independent validation, regulatory review, and domain expert oversight.
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+
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+ ## Recommended Machine Learning Setup
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+
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+ No official train, validation, or test split is provided in the repository. For reproducible research, users should report:
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+
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+ - The exact train, validation, and test split strategy
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+ - Random seeds
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+ - Image resizing and normalization settings
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+ - Data augmentation methods
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+ - Class balancing or reweighting strategy
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+ - Model architecture and pretrained weights, if any
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+
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+ Because 2,033 images are distributed across 33 classes, users should check for class imbalance and consider stratified splitting, augmentation, weighted losses, or balanced sampling.
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+
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+ ## Data Preprocessing
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+
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+ A typical transfer-learning preprocessing workflow is:
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+
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+ 1. Extract the archive.
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+ 2. Load images with a folder-per-class image loader.
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+ 3. Resize images to the model input size.
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+ 4. Normalize RGB channels using the selected model's expected preprocessing.
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+ 5. Use stratified train, validation, and test splits.
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+
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+ ## Limitations
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+
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+ - The dataset size is moderate for 33-class classification, so models may overfit without augmentation or transfer learning.
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+ - Class imbalance may affect evaluation metrics.
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+ - Results may depend strongly on image preprocessing, magnification, staining variation, and split strategy.
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+ - The dataset should be validated on external microscopy data before use in any applied biomedical setting.
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+
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+ ## Citation Requirement
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+
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+ If you use this dataset, derivative labels, trained models, figures, benchmarks, or results produced from this dataset, you **must cite both** of the following papers:
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+ 1. M. B. Jamshidi, D. T. Hoang, D. N. Nguyen, D. Niyato, and M. E. Warkiani, "Revolutionizing biological digital twins: Integrating internet of bio-nano things, convolutional neural networks, and federated learning," *Computers in Biology and Medicine*, vol. 189, p. 109970, 2025.
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+ 2. M. B. Jamshidi, S. Sargolzaei, S. Foorginezhad, and O. Moztarzadeh, "Metaverse and microorganism digital twins: A deep transfer learning approach," *Applied Soft Computing*, vol. 147, p. 110798, 2023.
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+
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+ ### BibTeX
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  ```bibtex
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+ @article{jamshidi2025revolutionizing,
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+ title={Revolutionizing biological digital twins: Integrating internet of bio-nano things, convolutional neural networks, and federated learning},
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+ author={Jamshidi, M. B. and Hoang, D. T. and Nguyen, D. N. and Niyato, D. and Warkiani, M. E.},
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+ journal={Computers in Biology and Medicine},
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+ volume={189},
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+ pages={109970},
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+ year={2025},
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+ publisher={Elsevier}
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+ }
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+
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  @article{jamshidi2023metaverse,
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  title={Metaverse and microorganism digital twins: A deep transfer learning approach},
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+ author={Jamshidi, M. B. and Sargolzaei, S. and Foorginezhad, S. and Moztarzadeh, O.},
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  journal={Applied Soft Computing},
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  volume={147},
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  pages={110798},
 
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  }
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  ```
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+ ## License
 
 
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+ This dataset is released under the MIT License.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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  ## Maintainer
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+ Mohammad Behdad Jamshidi
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+
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+ - Hugging Face: [MBJamshidi](https://huggingface.co/MBJamshidi)
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+ - GitHub: [MBJamshidi](https://github.com/MBJamshidi)