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PMC9429973
Results: From July 2018 until January 2022 we have treated with Ibrutinib third line 46 patients diagnosed with R/R CLL, 32 (69.6%) were males and 14 (30.4%) were females with a ratio M/F of about 2:1 with a median age of 63 ± 7.4 (range 43 – 80) years old.
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PMC11155445
Compound (101f) showed antibacterial activity against all the tested strains with MIC values in the range of 230–295 μg mL, whereas derivatives (101a, 101b and 101c) exhibited activity against two bacterial strains E. coli and B. cereus with MIC values in the range of 240–280 μg mL. Derivatives (101d) and (101e) showed activity against S. aureus and E. coli, respectively (Fig. 37).
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PMC11093197
A total of 0.3 × 10 cells were seeded onto the coverslips coated with poly‐L‐lysine solution (#RNBK1397, Sigma) in six‐well plates.
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PMC11760643
Absorbances of each well were read at 490 nm on a SpectraMax Microplate Reader (Molecular Devices) with the mean of medium‐only wells used to define background absorbances, which were subtracted from all other absorbance values.
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PMC11731614
Further experiments are required to validate all these cargo proteins, both in mice and human KDEVs, and to investigate their mechanism of action on nerve terminals.
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PMC11529598
Cells were incubated for indicated length of time (24 h, 48 h, 72 h 96 h).
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PMC11757230
Further studies are needed to analyze cell cycle-related genes and proteins in Ema cells exposed to the cADSC secretome, as well as to identify the specific secreted factors that may affect them.
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PMC11297139
These experiments were carried out with the approval of the Institutional Animal Care and Use Committee (IACUC) of Seoul National University.
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PMC10812665
For instance, mitochondrial or proteasome inhibitors have been characterized .
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PMC11548041
Aptamer-functionalized MNVs were used for the delivery of siRNA.
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PMC6163708
Important factors GLI1 and GLI2 were always expressed, only exceptionally one of them was lowered, suggesting the functionality of HH in all tumors tested.
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PMC9100622
The two approaches showed the MVA pathway as a major dependency in Ewing sarcoma and statin, an inhibitor of this pathway, as a potential new therapeutic agent for the treatment of Ewing sarcoma.
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PMC11754436
After co-transfection with DHRS4-AS1 and miR-362-5p mimic, DHRS4-AS1 reversed the high expression of miR-362-5p in MG63 cells, *Represent comparison with the mNC group, # represents comparison with the mm group. (
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PMC11461025
We determined that isogingketin exhibits anti-cancer effects on A375 cancer cells as well as further establish that this compound holds potential for use as an anti-cancer drug.
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PMC9429973
Moreover, SLe positive primary malignant BM plasma cells bind platelets, particularly from patients at relapse, suggesting that in vivo these cells may accumulate as the disease progresses and becomes resistant to treatment.
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PMC10482220
To distinguish between these possibilities, recombinant G3BP S149A was purified from baculovirus-infected insect cells and subjected to glutaraldehyde crosslinking.
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PMC3274243
Therefore, cell-free sensors were not only more susceptible to variation but this was also dependent upon the culture media employed.
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PMC8956657
The innate immune inhibition functions attributed to ORF4b include inhibition of interferon beta (IFNβ) and IFN lambda (IFNλ) production, as well as inhibition of cytokine production that is mediated by the NF-ĸB p50/p65 heterodimer.
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PMC11347429
Volcano plots were generated with the package EnhancedVolcano, version 1.8.0 (45).
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PMC11740508
Coverslips were PBS rinsed, blocked (PBS/2% BSA/1 h), fixed (3% formalin), and stained for CD44 (BD Pharmingen 561,860) to outline cell membranes, and DAPI to stain nuclear material.
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PMC11218145
Interestingly, TB was shown to block proton transport but not the ATP hydrolysis and thereby it was termed as “uncoupler” of the V-ATPase proton pumps.
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PMC11726848
No such changes were recorded after LDR irradiation.
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PMC6034753
MSB-1 lymphoblastoid cells are T-lymphocyte cell line, and are grown in suspension .
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PMC11488203
Culture of the breast carcinoma cells was performed using Leibowitz L-15 medium supplemented with 10% (v/v) fetal calf serum, 2 mM L-glutamine, 100U/ml penicillin and 100 µg/ml streptomycin (all from Sigma).
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PMC9429973
Aims: The aim of study was to compare the distribution of Duffy blood group antigens in individuals with and without COVID-19.
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PMC11730320
Thus, successful γδ gene rearrangements in the absence of pTCRα expression are more likely to result in the production of a mature γδ T cell than normal.
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PMC9429973
ATP1B3 is the smaller glycoprotein subunit (beta) of the sodium-potassium pump (Na/K-ATP pump).
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PMC9509578
Table 3 shows various nanomicelles and nanoemulsions that have been used to overcome cancer MDR.
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PMC11352877
This event increased microvessel density in pathological ovarian tissue .
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PMC9964654
All cell lines were grown in RPMI medium (Gibco) supplemented with 10% FBS (Gibco) and maintained in a humidified incubator (Heraeus, Hanau, Germany) with 5% CO2.
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PMC11657744
The asterisks (*) highlight primary cilia CEP89 deficiency delays ciliary disassembly in MEFs. (
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PMC9621239
Among these five patients, one (patient 11) was co-infected with genotypes B, C and D (Figure 1H), while the others (patients 4, 12, 13 and 23) were co-infected with genotypes B and C (Figure 1G, I-K).
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PMC9429973
It should be noted that 171 patients in the NPM1 intermediate group would be considered as favorable according to the ELN2017 criteria, as well as 162 patients in the NPM1 adverse group were previously classified as intermediate risk.
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PMC10706275
When developing a rCHO cell line through SSI, initial site selection is a crucial step in the process.
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PMC9268183
According to our results, five species were claimed to be used against cancer by locals (Table 1).
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PMC7504302
Horio et al. showed that siRNA-mediated knockdown of ZEB1 (zinc finger e-box binding homeobox 1) led to the suppression of proliferation, and anchorage-dependent and anchorage-independent clonal growth of MPM cells.
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PMC11683240
After that, cells were washed by centrifugation and 488-labeled PT bound to cell surfaces was measured using flow cytometry.
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PMC11568601
Then the cells were treated with increasing concentrations of TGT (20, 40, 60, 80, 100, 120, or 140 mg · mL).
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PMC9739791
For the A2780cisR cell line, cell viability after irradiation was 4.4% compared to 70% of survived cells in the absence of irradiation (Figure 7).
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PMC9429973
Methods: We used flow cytometry to characterize MRC2 expression in primary AML samples and healthy cells from the hematopoietic system.
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PMC11519077
Briefly, MSCs in the third passage were labeled with antihuman antibodies, including anti-CD73, anti-CD90, anti-CD34, and anti-CD45 antibodies, for flow cytometric analysis.
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PMC11361748
In a context-dependent fashion, for example, the cytoplasmic pool of K17 regulates protein synthesis and keratinocyte growth , TNFα-induced apoptosis , and inflammatory/immune gene expression , while a small nuclear pool of K17 regulates nuclear architecture , gene expression , the cell cycle , and the DNA damage and repair response .
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PMC11725127
Our work highlights the potential of USP2 as a promising therapeutic target in MM and sheds light on the treatment of cancers with KRAS mutations.
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PMC11711127
Initiation of the intrinsic apoptotic pathway is regulated by the balance between pro- and anti-apoptotic Bcl-2 proteins expressed in the T cell.
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PMC8358006
In addition, the broad H3K4me3 domains have been found to associate not only with increased transcriptional precision of cell-type-specific genes related to cell identity but also with the increased transcriptional elongation and enhancer activity of tumor-suppressor genes.
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PMC8759873
These factors are called angiogenic factors.
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PMC11033180
of cellsRi steady state (Mo)Rheobase (pA)AP Frequency (Hz) +30pAAP amplitude (mV)AP duration (ms)AP rise time (ms)AP decay time (ms)Fc10867,3 ± 57,0927,5 ± 2,8145 ± 1,01154,41 ± 1,8710,45 ± 1,2715,08 ± 0,76575,371 ± 0.614Sema5A-Fc12973,3 ± 41,4425,42 ± 4,4585,833 ± 1,38143,87 ± 2,00211,18 ± 0,84185,857 ± 0,63785,233 ± 0,4715p-value0,14030,71030,87590,00110,62660,44090,8588 Sema5A impacts connectivity and synaptic properties in enteric neurons Electrophysiological properties of enteric neurons cocultured for 3 days with COS cells expressing Fc or Sema5A-Fc, were measured in the cell-attached and whole-cell configuration. (
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PMC8193046
The standard ABCC1 inhibitor 26 was synthesized as described earlier .
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PMC9429973
Y. Fang, C.-K. Chang Department of Hematology, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital; State Key Laboratory of Medical Genomics, National Research Center for Translational Medicine at Shanghai, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China Background: Mutations of TP53 gene confers inferior prognosis in myelodysplastic syndromes.
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PMC11525293
After 24 h from transfection, cell extracts were prepared and analyzed by WB with anti-ACE2 and anti-p63 Abs.
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PMC9164404
Treatment groups were: (1) PBS, (2) cisplatin (3.5 mg Pt/kg), (3) Abplatin (3.5 mg Pt/kg).
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PMC9429973
Subjects deriving clinical benefit could rollover into an extension study after 28 days, and C5 INH could be withdrawn.
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PMC6312945
Wei et al reported that HBV protein can downregulate the expression of miR-101 (9).
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PMC9688728
Unstained or treated with non-specific isotypic antibodies conjugated with PE samples served as negative controls.
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PMC11740508
C M231 xenografts.
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PMC9429973
Median age was 42 years old (0-69) (43 for GCV and 39 for C, p=0.001) and 59% were man (56.7% for GCV and 59.3% for C, p=0.517).
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PMC10178190
In tumor cells, ectopic expression of PBX1 promotes cancer stem cell-like phenotypes, including platinum chemoresistance.
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PMC10362265
Comparison of two PDB structures of human IL18, the one (PDB ID: 3wo2) , which was recruited to docking and the reference structure (PDB ID: 7al7), showed some variations as well.
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PMC11703992
DDIT4 expression in the modified Jurkat cell lines was confirmed by Western blot analysis. (
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PMC9688728
In addition, most of the dicarboxylic acids and alcohols found in the experiment (four of five in both cases) belong to this group.
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PMC10976516
This would then consequently link carrier-immune cell effector functions with OV-induced oncolysis.
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PMC10178190
Our data are consistent with other reports, demonstrating that exposure of OC cells to IL-6 promotes cisplatin resistance, accompanied by elevated pSTAT3 levels.
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PMC11387401
The normalized relative quantities of the target gene were determined with quantitative PCR 48 h post ASO treatment and 48 h post infection.
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PMC10523483
Among five somatic H1 variants, H1C is the most dominantly expressed variant in NSCLC tumors (Fig. 1B and SI Appendix, Fig. S1 A–E).
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PMC10854447
Compounds 7j, 7k, and 7 l, the most potent derivatives in all in vitro studies, were tested for their capacity to initiate the apoptosis cascade and reveal their proapoptotic potential.
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PMC11347429
Analysis and visualization of expression data was performed using the R software package (version 4.2.1, (38) and RStudio (version 2022.07.2, (39)).
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PMC9621239
Sn, Shannon entropy.
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PMC10079526
γTubulin staining was used as a control.
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PMC9545474
HRMS‐ESI: calculated [M+H] 638.05532 m/z, found 638.05772 m/z.
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PMC9583612
Thus, while it is true that aggregate measurement-based estimates of emissions are higher than inventory estimates, they are not sufficient for site-specific inventory development.
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PMC11605551
GDC519 were transplanted into the right cerebral cortex of immunodeficient mice and when the mice got tumors, the right and left cerebrum were dissected separately.
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PMC11750696
Proteins in green may promote indicated process, while protein in red may suppress its activation or differentiation.
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PMC6024716
These observations can be explained by the known role of OAZ1 as a negative regulator of cell growth , meaning its absence might offset the possible negative effects on cell growth engendered by improved protein expression.
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PMC11754436
Fig. 1Regulation of DHRS4-AS1 on OS cell proliferation and apoptosis in vitro. (
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PMC11657695
The physiological saline solution (PSS) had the following composition (in mM): NaCl 140, MgCl2 1, KCl 4, CaCl2 2, D-glucose 11.1 and HEPES 10, adjusted to pH 7.4 with NaOH.
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PMC11502327
Limitations of natural product chemistry include low water solubility and limited availability of targeted organs, which may limit their therapeutic use in clinical settings (249–251).
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PMC11718809
In this study, the circRNAs detected in the mammary tissue of yaks mainly originated from exons, which is consistent with results from the mammary tissue of Holstein cows , sheep , and goats .
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PMC8956657
A summary cartoon is presented, highlighting the minor and major site interactions and the structure presented in the right panel.
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PMC11617590
By treatment with 100 μM SFN for 24 h, the expression of BCL‐2 was decreased (0 μM SFN; 1.00 ± 0.11 vs. 100 μM SFN; 0.59 ± 0.06) (**p < 0.01, Figure 6).
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PMC11786767
Ki-67 knockdown or reducing Ki-67 phosphorylation by means of a cyclin-dependent kinase 1 (CDK1) inhibitor disrupts the distribution of nuclear lipids at the perichromosomal layer.
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PMC8584666
Cysteamine is known to inhibit glutathione synthesis, resulting in intracellular oxidation.
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PMC9429973
Mosunetuzumab (M) is a T-cell engaging CD20xCD3 bispecific monoclonal antibody that redirects T cells to eliminate malignant B cells (Sun, et al. 2015; Budde, et al. 2022).
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PMC11802929
We used the SCT identity to connect Pep-Groups with gene expression.
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PMC11723210
The final stage performs multi-wavelength phase recovery to achieve accurate phase reconstruction.
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PMC11640419
The Luminex xMAP intracellular protein assay is a multiplex multiparameter assay that employs a multiplex of fluorescent-labeled antibodies and magnetic particles to enhance the sensitivity, dynamic range, and accuracy of the analytical method.
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PMC9429973
Results: Our results showed that GAS5 expression level in AML patients was lower compared to healthy controls (p < 0.01).
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PMC4816271
Functional experiments were performed next, and there were clear differences in the abilities of the spacer variants to mediate cytotoxicity, cytokine release and proliferation in response to Lan-1 cells.
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PMC9429973
Interestingly, patients with high cfDNA TF at baseline were more likely to present with extramedullary disease (EMD) and a higher number of focal lesions, and also featured a more active tumour metabolism, as compared to pts with low TF (EMD 4/44=9% vs. 1/44=2.3%, p=ns; M n. PET lesions: 1.7 vs. 2.5, p= 0.003; SUVmax: 5.2 vs. 9.6, p = 0.01).
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PMC10798140
Other quinoline compounds have been shown to disrupt genes involved in immune regulation and inflammation.
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PMC10123657
In this regard, it has been reported that the dissociation of cells from the BM, spleen, or lymph nodes caused fragmentation of resident macrophages, and that these fragments, attached to hematopoietic cells, contaminated single-cell suspensions .
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PMC11634027
Blots were washed four times (10 min each) with PBT (1×PBS+0.01% Tween-20), then incubated with secondary antibodies anti-mouse IgG-800 (LI-COR Biosciences; 1:20,000) and anti-rabbit IgG-680 (LI-COR Biosciences; 1:20,000) for 1 h at room temperature.
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PMC11763126
Fig. 5CBDP1 Inhibits RCC Progression by Regulating TRPM3.
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PMC8751435
As Figure 3a shows, amount of condensing point, which represented DNA damage, was increased notably in a dose‐dependent manner after treatment with CME when compared with control.
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PMC11723210
Considering wavelength-scanning-based LFOCM does not require complicated and time-consuming mechanical operations, we applied our method to it to verify the effectiveness of the BPR method.
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PMC11798926
One possibility is that surface SynDIG4 could be localized extrasynaptically to stabilize pools of GluA1.
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PMC11766174
In those experiments, approximately 100,000 cells were scanned per donor per condition, and less than 0.1% CA-positive cells were detected by CA-ICC in the vehicle control treatment (DMSO), while P/I stimulation increased the proportion of CA-positive cells to ~1% (Figure 5A).
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PMC9504875
Full-length RIG-I was ubiquitinated by SeV infection (Figure 5C, lane 3), consistent with the previous report by Gack et al. .
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PMC10938377
Chemical reagents were purchased from commercial sources (Sigma Aldrich, ABCR, Acros, and TCI Chemicals) and used without further purification.
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PMC11754291
Three types of conditioned medium were used: CM (keratinocyte medium only), CMK (medium from non-irradiated keratinocytes), and CMiK (medium from irradiated keratinocytes).
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PMC11509224
Antibacterial experiments were performed on Mycobacterium smegmatis NBRC 3207 using the paper disc method.
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