PMCID
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PMC8903741
Given the fact that the antibody from #6-5 displayed a greater increased ELISA titer than did those of #6-9, we additionally purified #6-5 using PA affinity chromatography (Fig. 4C) and eventually confirmed the antigen-binding activity of the antibody with various concentrations of hMMP9.
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PMC11423992
Error bars represent the standard deviation from five independent experiments means.
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PMC11695623
Differential analysis of DCUN1D5 was performed using the DESeq2 package and edgeR package of the R software.
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PMC9817179
The images displayed in the article are maximum z-projections of z-stack images.
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PMC6222635
Polysaccharides with a smaller molecular weight will be present after absorption.
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PMC8658661
Among the cell culture mentioned above, the HepG2 cell line has gained popularity due to its wide range of applications in scientific research.
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PMC10243817
Taken together, these data indicate that MEX3B can bind to the AAAAAAA motifs located in the 4385 to 4405 sites and the 5416 to 5422 sites in the 3′ UTR of TGFBR3 mRNA and destabilize the mRNA, thus downregulating TGF-βR3 expression.
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PMC11502962
These results suggest that the MIF/CXCR4 axis could play a key role in GIST progression.
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PMC4816271
Each line represents one mouse.
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PMC9370419
After being treated with drugs for three days, the total cellular RNA from 12-well plates was lysed using TRIzol reagent (Invitrogen) following the instruction manual.
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PMC11587606
G-J) The mRNA expression of RHBDF1 (G), Occludin (H), ZO-1 (I) and E-Cadherin (J) in Dox stimulated TetON-RH in 3D acini cultures (n = 3).
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PMC9351137
In addition, we found an extrachromosomal localization of histone H2A.Z to the isolated midbodies.
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PMC11343332
Pycnogenol was applied to the MCF-7 cells in a range of concentrations (20-120 µg/ml) for 24 hours.
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PMC9429973
Reasons for anticoagulation: surgery, catheters, cancer, heart disease and vasculitis.
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PMC11681005
Taken together, our results suggest that during BLCA oncogenesis, AR binding reprogrammed DNA hypomethylation occurs concomitantly with epigenomic patterning to control specific transcription programs, which may drive the evolutionary heterogeneity of MIBC.
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PMC11387401
Thus, to understand the complete virus-host interplay, it is crucial to uncover the RNA interaction partners of all HIV-1 proteins.
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PMC11271278
Coverslips with fixed cells in metaphase were washed twice for 5 min with 2xSSC.
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PMC9429973
Schulman S, et al. J Thromb Haemost.
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PMC11521786
Both preparations were adjuvanted in 200 μg of VSSP per mouse and emulsified with Montanide ISA 51 VG.
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PMC11015054
The results of METTL5 expression in cancer and paracancerous tissues based on immunohistomicroarrays of 80 cases showed that the same expression of METTL5 was upregulated in HCC (p < 0.01) (Figure 1B), and to assess the effect of METTL5 on survival prognosis, METTL5 expression levels were compared between patients in high‐ and low‐expression groups, results showed that high‐expression patients had a shorter OS and DFS than low‐expression patients (Figure 1E,F).
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PMC11566417
The anti-MRSA effect observed with MDEC CM was not recapitulated using CM of any of these three additional bovine MSCs (Supplemental Fig. 3).
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PMC11473750
To determine the mutations responsible for the phenotype, sequence-specific assays were designed using the Primer Express™ software, Version 3.0.1 from Applied Biosystems (Thermo Fisher Scientific, Australia), based on the location of the single nucleotide variation identified by exome sequencing.
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PMC11055323
f Venn plot shows the intersection of cohesin component RAD21 binding sites in BJ cells with the overexpression of SS18-SSX1 and SS18(YA)-SSX as control.
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PMC11704135
Viable microorganisms interacted with the TTC solution, resulting in a color change from no color to pink.
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PMC11423992
The binding activity of TFAB002s to CD20-expressing WIL2-S cells, CD3-expressing Jurkat cells, FcRn-expressing H_FCGR2B CHO-K1 cells, parent cell line CHO-K1 cells, and the CD20 expression level on WIL2-S and JeKo-1 cells were evaluated.
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PMC11583690
This indicates that although BPMO's average particle size (approximately 50 nm) is smaller than MSN's (100 nm) DTX's loading capacity onto BPMO is comparable to that of MSN.
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PMC11442172
Given the metabolic differences identified between 2D and 3D culture conditions, it is not surprising that these models show altered sensitivities to commonly used therapeutic agents.
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PMC10747160
The three sugar-binding moieties on one of the Griffithsin monomer domains were removed via mutagenesis, yielding a constitutively dimeric form of Q-Griffithsin with only one monomer domain being able to bind to sugars (Figure 3A).
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PMC9545714
These genes include ELOV4, CYP4F22, PNPLA1, and ABHD5.
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PMC3675084
Anti-class III β-tubulin antibody was obtained from Promega, anti-P-glycoprotein mAb (C219) was purchased from Calbiochem.
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PMC11732628
Disrupted cell-cycle regulation is a typical characteristic of tumor advancement (33).
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PMC11323699
By way of example, Gilead’s anti-CD47 mAb magrolimab was granted breakthrough drug designation in 2020 and later put on hold by the FDA due to severe side effects in clinical trials .
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PMC11514418
Among them, 41 patients received APS injection combined with GS chemotherapy, and 56 patients received GS chemotherapy only.
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PMC11531744
Differentially expressed immune-related genes between normal and tumor tissues of the train dataset were identified using the Wilcoxon test (FDR < 0.05, |Log2(fold-change)| > 1).
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PMC11805741
Astaxanthin demonstrated a ΔG of -4.75 kcal/mol, indicating a moderate binding affinity with BCL-2.
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PMC11126803
The relative sequences of the primers are listed in Table S3A.
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PMC9429973
In group I, regardless of the distribution of the 6 α-globin genes, homozygous triplication (ααα/ααα) or heterozygous quadruplication (αααα/αα), association with heterozygous β-thalassemia results in anemia of moderate to severe that may or may not require transfusion therapy, it is the severity of the HBB gene mutation that would determine the clinical variation.
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PMC11429241
The scaffolds are the key components that provide a 3D environment for cells to grow and interact with each other and their surroundings .
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PMC9429973
Effects of eltrombopag on cellular immune function: there were no differences in the percentages of Treg cells, B lymphocytes, Th1, and Th2 cells between before and after treatment。But the group with higher pretreatment levels of Treg cells had better hematologic responses than the group with low levels of Treg cells (P < 0.05).
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PMC11496728
To verify the increase of the expression level of the recombinant protein by the addition of regulatory elements, IL-3 was replaced with SEAP, and three expression vectors pCMV-IL3, pCMV-IL3-1, and pCMV-IL3-2 were successfully constructed (Fig. S1G–I).
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PMC10907726
Data are shown as means ± SD; n = 3 per group.
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PMC10734950
Thirteen hundred compounds were screened from the ChemBridge DIVERset library using the CCRF-CEM cell line.
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PMC7802142
miRNA profiling analysis revealed that miR-100 was much more abundant in ReN-NV than 293-NV.
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PMC7612475
B, Fold change in tumor volume in mice from the different treatment groups described above.
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PMC11514418
Baseline information, n (%) APS: Astragalus polysaccharide; GS: Gemcitabine plus tegafur gimeracil oteracil potassium capsule (S-1); TNM: Tumor node metastasis classification.
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PMC9370419
Then, the docking with estradiol benzoate was conducted using AutoDock 4 and AutoDockTools with the predicted model 1 of HBx .
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PMC11699178
PCA was applied for linear dimensionality reduction based on these genes, showing the distribution of cells in PC_1 and PC_2 (Figure S1C and Figure S1D).
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PMC10976516
Initial MTD and safety/toxicity evaluation.
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PMC5963610
Therefore, we are unable to study the effect of combination therapy on these models too.
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PMC11756907
Human recombinant protein tyrosine phosphatase PTP1B was purchased from Sigma-Aldrich.
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PMC11743316
Apoptosis is a closely regulated physiological process required for appropriate embryonic development, genomic integrity preservation, immune system function, and tissue homeostasis maintenance.
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PMC11478724
The data are presented as mean values and SDs from three testing.
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PMC11541241
As such, scientists have employed diverse strategies to manipulate the TOR pathway and combat plant pathogens.
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PMC11599565
Green arrows denote an upregulation, red arrows a downregulation at the transcriptional level.
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PMC5415764
These formulations with drugs loaded in LPNs generated higher apoptosis rates than the free P+T combination (Fig. 6), indicating the advantages of utilizing iRGD on LPNs and the co-delivery of drugs.
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PMC11680562
Stability chambers configured at these conditions were utilized to store gel samples.
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PMC10761571
For this purpose, the development of supermagnetic NPs for site-directed delivery of drugs has been considered.
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PMC2193049
The samples were subsequently lyophilized.
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PMC11574271
The findings suggest that miR-659-3p serves as a potent negative regulator in an in vivo setting, exerting a substantial impact on the advancement of bladder cancer xenografts that express RON.
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PMC11574271
Scale bar 1 µm).
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PMC9429973
The tumor was completely resected at diagnosis in 17 patients (22.7%), while a residual tumor existed after biopsy in 58 patients (77.0%).
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PMC11599565
The MAP4K4i revealed ambiguous expression of HES1 in different OC cells.
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PMC11774251
Quantification of apoptotic cells (%) under different treatment conditions. (
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PMC11590870
However, more methodologies can be used to assess their antioxidant potential.
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PMC11768927
Additionally, Taha et al. explored the combination of VSVΔ51-HER2T, an rVSV expressing a truncated HER2 to be targeted by trastuzumab, with a vaccinia recombinant expressing an HER2-targeted T-cell engager (this TCE contains an HER2-specific trastuzumab single-chain variable fragment [scFv] fused to CD3-specific scFv).
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PMC11711063
To quantify glucose uptake in vitro, the Cell Meter™ 2‐NBDG Glucose Uptake Assay Kit was used.
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PMC11726848
In the experiments with LDR irradiation, both the control and irradiated cells were located on one culture plate.
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PMC11739504
The graph on the bottom right indicated fluorescence intensity of ciliary IFT52.
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PMC11361748
Measurements of selected cytokines and chemokines in CM were performed by RayBiotech and Immunology Core at University of Michigan.
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PMC9429973
A 3 + 3 design was utilized with Ven.
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PMC10747160
Griffithsin can be synthesized economically either in plants or bacteria, has been shown to have low toxicity in mammals, can withstand temperatures up to 78 °C, maintains inhibitory capability after several weeks of exposure to 50 °C, can withstand at least five freeze–thaw cycles, and survives exposure to a variety of acid and base solutions for at least 24 h .
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PMC11509278
During the experiment, the propolis solution was diluted with the complete culture medium to the required concentration for further experiments.
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PMC11790599
β-actin was used as endogenous loading control for normalization.
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PMC10204952
S1P synthesized by SphK1 is transported out of the cells to interact with a family of cell-surface G protein-coupled receptors (S1PR1-5).
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PMC7642379
However, the adaptation of cells from adherent to suspension growth and the differential cultivation procedures between adherent and suspension cells induces phenotypic changes to the cell lines.
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PMC11684821
A similar tyrosine was present in 24 of 27 of the TCR-α chains we describe where it was encoded by TRAJ26, TRAJ29, TRAJ32, TRAJ38, TRAJ39, TRAJ47, and TRAJ12 or TRAJ20, which are sometimes used by MAIT cell TCRs to provide a similarly placed tyrosine residue, which makes important contacts with MR1 and its bacterially derived cargo (25).
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PMC9429973
Methods: We conducted a single center retrospective study to investigate the prognostic role of RDW in children with JMML.
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PMC11696576
GK420 was the most potent compound, inhibiting 97% of AA release at 1 μM with an IC50 of 0.09 µM. Combined with favorable selectivity and stability it was thus chosen for further evaluation.
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PMC11474209
These pathways usually compete to heal a lesion, preventing it from becoming stably mutated cells.
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PMC9436459
Preliminary mechanism of action showed that these compounds could damage the bacterial cell membranes as well as inhibit the efflux of drugs such as methicillin and ethidium bromide .
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PMC11700165
It has a highly efficient phosphorylation capacity for the B- and T cell adapter proteins BLNK (B cell linker), also known as SLP-65, and for SLP-76 (SH2 domain containing leukocyte protein of 76 kDa), respectively.
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PMC9739791
Subsequent ICP-MS assay demonstrated that Pt content in mitochondria was 4.1-folds higher than in the nucleus.
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PMC11687167
JAK/STAT promotes the differentiation of Th cells and the expansion of Tregs.
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PMC10759659
First, Hep3B and SK-HEP-1 cells were infected with lentivirus containing polybrene (10 μg/ml) (TR-1003; Sigma‒Aldrich) for 24 h. Then, positive cells were selected with 100 μg/ml hygromycin B. The efficiency of ARID1A knockout was confirmed by Western blotting.
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PMC10198699
Accordingly, although a tissue sample might not have been susceptible of ownership in Roman times (because the Romans did not have much use for it), a tissue sample that is currently used for research is indeed susceptible of ownership because it is (a) useful and valuable in research, (b) removed and hence separated from a human body, and (c) capable of human control.
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PMC11650848
Cell line pairs with opposite methylation patterns were used.
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PMC8740518
However, there are few metabolomics studies of MM.
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PMC6160470
After washing the membranes with TBST, membranes were incubated with HRP conjugated secondary antibody (anti-mouse IgG-HRP, 1:10000, GE Healthcare or anti-rabbit IgG-HRP, 1:5000, Bio-Rad) for 2 hours at room temperature and subsequently washed again with TBST before detection of signal by Western Lightning Plus-ECL reagent (Perkin-Elmer, USA).
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PMC11013014
HRMS (+ESI) m/z: [M + H] calculated for C23H21Cl2N6O5: 531.0945, found, 531.0943.
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PMC11429241
These cultures cannot mimic the biological, chemical, and mechanical cues present in the three-dimensional architecture of a primary tumor .
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PMC11464982
The number in bold represents the maximum recommended use level, which was used for calculation.
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PMC11591038
HIF-1α and HIF-1β each contain the structure of basic helix-loop-helix-PAS (bHLH-PAS) domains that are crucial for heterodimerization and DNA binding .
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PMC10719213
Phosphorylation of cladribine to its active triphosphate form (2-CdATP) is regulated by enzymes of the deoxyribose nucleotide salvage pathway, required for DNA synthesis .
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PMC11001582
Graph, mean ± s.e.m.;
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PMC11486946
EGFR fractions shifted from small to large oligomers with higher EGF concentrations.
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PMC9429973
Marker positivity was defined as variant allele frequency above the limit of detection as not all patients were in complete remission (CR), in CR with incomplete hematological recovery (CRi) or in morphologic leukemia free state (MLFS).
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PMC11127909
Briefly, OS cells (1 × 104 cells/well) were seeded into 96-well Seahorse XF96 culture plates in DMEM containing 10% FBS and were incubated at 37 °C in a non-CO2 incubator overnight.
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PMC9504875
The N-terminal region of N protein and the C-terminal region of TRIM25 are important for their interaction.
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PMC8414691
B From left to right: normal metaphase (mock), multipolar spindle (RNAi), and chromosome misalignments (RNAi).
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PMC11588085
The histological grade indicates cell differentiation, with Grade 1 for well-differentiated tumor cells, Grade 3 for poorly differentiated cells, and Grade Borderline (GB) for noncancerous ovarian tumors.
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