# Paired Refinement Experiments

This directory evaluates masked diffusion as an enhancer refiner rather than as a pure de novo generator.

For each reference sequence `x`, the script creates edited variants `x'` under a mutation budget `rho`, scores both sequences with the trained DeepSTARR predictor, and reports:

```text
delta = predictor(x') - predictor(x)
```

## Experiments covered

1. Paired delta distribution over `rho = 0.01, 0.03, 0.05, 0.10, 0.20`.
2. Mask-selection strategy comparison:
   - `random`: randomly chosen editable positions.
   - `entropy`: positions where the diffusion model is most uncertain.
   - `logit_gap`: positions where the diffusion model most prefers a different base than the current base.
3. Best-of-N refinement:
   - sample multiple edits per sequence and keep the candidate with the largest predicted activity gain.

## Outputs

The default output directory is:

```text
${RESULT_ROOT}/refinement/paired_refinement_test
```

It contains:

```text
refinement_details.jsonl
refinement_summary.json
paired_delta_by_mask_ratio.png
strategy_delta_violin.png
best_of_n_refinement_curve.png
```

## Recommended command on the GPU server

Run from the repository root. Use explicit environment variables to avoid stale defaults from `env.sh`.

```bash
cd /inspire/hdd/project/intelligentcreativedesign/dangshengqi-253114050252/z-anna/genrl-enhancer-diffusion

PROJECT_ROOT=$(pwd) \
RUN_ROOT=$(pwd)/paper_runs \
DEEPSTARR_DIR=$(pwd)/datas/DeepSTARR-enhancer-activity \
GENERANNO_BASE_MODEL=$(pwd)/models/GENERanno-eukaryote-0.5b-base \
DIFFUSION_MODEL=$(pwd)/saved_model/deepstarr_discrete_diffusion \
PREDICTOR_MODEL=$(pwd)/paper_runs/results/deepstarr_regression/best_model \
REFINEMENT_SPLIT=test \
REFINEMENT_NUM_SEQUENCES=1024 \
REFINEMENT_NUM_SAMPLES=8 \
REFINEMENT_MASK_RATIOS="0.01 0.03 0.05 0.10 0.20" \
REFINEMENT_STRATEGIES="random entropy logit_gap" \
REFINEMENT_DIFFUSION_STEPS=32 \
REFINEMENT_BATCH_SIZE=16 \
PREDICTOR_SCORE_BATCH_SIZE=256 \
TRANSFORMERS_NO_TF=1 \
USE_TF=0 \
TOKENIZERS_PARALLELISM=false \
nohup bash scripts/11_refinement/run_paired_refinement.sh > paired_refinement.log 2>&1 &
```

For a fast smoke test:

```bash
PROJECT_ROOT=$(pwd) \
RUN_ROOT=$(pwd)/paper_runs \
DEEPSTARR_DIR=$(pwd)/datas/DeepSTARR-enhancer-activity \
GENERANNO_BASE_MODEL=$(pwd)/models/GENERanno-eukaryote-0.5b-base \
DIFFUSION_MODEL=$(pwd)/saved_model/deepstarr_discrete_diffusion \
PREDICTOR_MODEL=$(pwd)/paper_runs/results/deepstarr_regression/best_model \
REFINEMENT_SPLIT=valid \
REFINEMENT_NUM_SEQUENCES=64 \
REFINEMENT_NUM_SAMPLES=2 \
REFINEMENT_MASK_RATIOS="0.03 0.10" \
REFINEMENT_STRATEGIES="random logit_gap" \
REFINEMENT_DIFFUSION_STEPS=16 \
REFINEMENT_BATCH_SIZE=16 \
PREDICTOR_SCORE_BATCH_SIZE=128 \
TRANSFORMERS_NO_TF=1 \
USE_TF=0 \
TOKENIZERS_PARALLELISM=false \
nohup bash scripts/11_refinement/run_paired_refinement.sh > paired_refinement_smoke.log 2>&1 &
```