# Copyright 2009 by Michiel de Hoon. All rights reserved. # This code is part of the Biopython distribution and governed by its # license. Please see the LICENSE file that should have been included # as part of this package. """Code for calling and parsing ScanProsite from ExPASy.""" from urllib.parse import urlencode from urllib.request import urlopen from xml.sax import handler from xml.sax.expatreader import ExpatParser # October 28th 2020 it was recognised that between October 10th 2020 and October # 28th the main url of prosite changed from https://www.expasy.org to # https://prosite.expasy.org. Thus a change in the mirror was issued from # https://www.expasy.org to https://prosite.expasy.org. PROSITE_URL = "https://prosite.expasy.org" class Record(list): """Represents search results returned by ScanProsite. This record is a list containing the search results returned by ScanProsite. The record also contains the data members n_match, n_seq, capped, and warning. """ def __init__(self): """Initialize the class.""" self.n_match = None self.n_seq = None self.capped = None self.warning = None def scan(seq="", mirror=PROSITE_URL, output="xml", **keywords): """Execute a ScanProsite search. Arguments: - mirror: The ScanProsite mirror to be used (default: PROSITE_URL). - seq: The query sequence, or UniProtKB (Swiss-Prot, TrEMBL) accession - output: Format of the search results (default: xml) Further search parameters can be passed as keywords; see the documentation for programmatic access to ScanProsite at https://prosite.expasy.org/scanprosite/scanprosite_doc.html for a description of such parameters. This function returns a handle to the search results returned by ScanProsite. Search results in the XML format can be parsed into a Python object, by using the Bio.ExPASy.ScanProsite.read function. """ if output != "xml": raise NotImplementedError("Only output='xml' is supported currently.") parameters = {"seq": seq, "output": output} for key, value in keywords.items(): if value is not None: parameters[key] = value command = urlencode(parameters) url = f"{mirror}/cgi-bin/prosite/scanprosite/PSScan.cgi?{command}" handle = urlopen(url) return handle def read(handle): """Parse search results returned by ScanProsite into a Python object.""" content_handler = ContentHandler() saxparser = Parser() saxparser.setContentHandler(content_handler) saxparser.parse(handle) record = content_handler.record return record # The classes below are considered private class Parser(ExpatParser): """Process the result from a ScanProsite search (PRIVATE).""" def __init__(self): """Initialize the class.""" ExpatParser.__init__(self) self.firsttime = True def feed(self, data, isFinal=0): """Raise an Error if plain text is received in the data. This is to show the Error messages returned by ScanProsite. """ # Error messages returned by the ScanProsite server are formatted as # as plain text instead of an XML document. To catch such error # messages, we override the feed method of the Expat parser. # The error message is (hopefully) contained in the data that was just # fed to the parser. if self.firsttime: if data[:22].decode("utf-8") != "": raise ValueError(data) self.firsttime = False return ExpatParser.feed(self, data, isFinal) class ContentHandler(handler.ContentHandler): """Process and fill in the records, results of the search (PRIVATE).""" integers = ("start", "stop") strings = ( "sequence_ac", "sequence_id", "sequence_db", "signature_ac", "level", "level_tag", ) def __init__(self): """Initialize the class.""" self.element = [] def startElement(self, name, attrs): """Define the beginning of a record and stores the search record.""" self.element.append(name) self.content = "" if self.element == ["scanprosite_response", "matchset"]: self.record = Record() self.record.n_match = int(attrs["n_match"]) self.record.n_seq = int(attrs["n_seq"]) elif self.element == ["scanprosite_response", "matchset", "match"]: match = {} self.record.append(match) def endElement(self, name): """Define the end of the search record.""" assert name == self.element.pop() if self.element == ["scanprosite_response", "matchset", "match"]: match = self.record[-1] if name in ContentHandler.integers: match[name] = int(self.content) elif name in ContentHandler.strings: match[name] = self.content else: # Unknown type, treat it as a string match[name] = self.content def characters(self, content): """Store the record content.""" self.content += content