| 1. In a sterile 0.2 mL PCR tube, add Master Mix (in the tube labeled M). | |
| 2. Add Forward Primer (in the tube labeled F). | |
| 3. Add Reverse Primer (in the tube labeled R). | |
| 4. Add template DNA (in the tube labeled T). | |
| 5. Mix contents by gently flicking the tube, then perform a quick spin in a microcentrifuge to collect the liquid at the bottom. | |
| 6. Place the tube into the thermocycler. |