improve formatting and add call to regster

README.md

@@ -30,8 +30,14 @@ cDNA display proteolysis of natural and designed proteins from which 776,298 hig
 stabilities covering all single amino acid variants and selected double mutants of 331 natural
 and 148 de novo designed protein domains 40–72 amino acids in length.
 
+*** **IMPORTANT! Please [register your use](https://forms.gle/wuHv8MKmEu4EEMA99) of these data so that we (the Rocklin Lab) can continue to release new useful datasets!! This will take 10 seconds!!** ***
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 ## Quickstart Usage
 
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 ### Install HuggingFace Datasets package
 
 Each subset can be loaded into python using the Huggingface [datasets](https://huggingface.co/docs/datasets/index) library.
@@ -109,27 +115,27 @@ XXX **designed targets** were selected from
 ### Library construction
 The cDNA proteolysis screening was conducted as four giant synthetic DNA oligonucleotide libraries
 and obtained K50 values for 2,520,337 sequences, 1,841,285 of these measurements are included here:
-    * Library 1:
-      * ~250 designed proteins and ~50 natural proteins that are shorter than 45 amino acids
-      * padding by Gly, Ala and Ser amino acids so that all sequences have 44 amino acids
-      * ~244,000 sequences Purchased from Agilent Technologies, length 230 nt.
-    * Library 2:
-      * ~350 natural proteins that have PDB structures that are in a monomer state and have 72 or less amino acids after removing N and C-terminal linkers
-      * padding by Gly, Ala and Ser amino acids so that all sequences have 72 amino acids
-      * ~650,000 sequences
-      * also includes scramble sequences to construct unfolded state model.
-      * Purchased from Twist Bioscience, length 250 nt.
-    * Library 3:
-      * ~150 designed proteins
-      * comprehensive deletion and Gly or Ala insertion of all wild-type proteins included in Library 1 and Libary 2
-      * amino acid sequences for comprehensive double mutant analysis on polar amino acid pairs
-      * ~840,000 sequences
-      * Purchased from Twist Bioscience, length 250 nt.
-    * Library 4:
-      * Amino acid sequences for exhaustive double mutant analysis on amino acid pairs located in close proximity
-      * overlapped sequences to calibrate effective protease concentration and to check consistency between libraries
-      * ~900,000 sequences
-      * Purchased from Twist Bioscience, length 300 nt.
+  * Library 1:
+    * ~250 designed proteins and ~50 natural proteins that are shorter than 45 amino acids
+    * padding by Gly, Ala and Ser amino acids so that all sequences have 44 amino acids
+    * ~244,000 sequences Purchased from Agilent Technologies, length 230 nt.
+  * Library 2:
+    * ~350 natural proteins that have PDB structures that are in a monomer state and have 72 or less amino acids after removing N and C-terminal linkers
+    * padding by Gly, Ala and Ser amino acids so that all sequences have 72 amino acids
+    * ~650,000 sequences
+    * also includes scramble sequences to construct unfolded state model.
+    * Purchased from Twist Bioscience, length 250 nt.
+  * Library 3:
+    * ~150 designed proteins
+    * comprehensive deletion and Gly or Ala insertion of all wild-type proteins included in Library 1 and Libary 2
+    * amino acid sequences for comprehensive double mutant analysis on polar amino acid pairs
+    * ~840,000 sequences
+    * Purchased from Twist Bioscience, length 250 nt.
+  * Library 4:
+    * Amino acid sequences for exhaustive double mutant analysis on amino acid pairs located in close proximity
+    * overlapped sequences to calibrate effective protease concentration and to check consistency between libraries
+    * ~900,000 sequences
+    * Purchased from Twist Bioscience, length 300 nt.
 
 
 ### Bayesian Stability Analysis