| <table border="1"> | |
| <tr><td>Rank</td><td>Athlete</td><td>Nationality</td><td>Time</td><td>Notes</td></tr> | |
| <tr><td>1</td><td>Félix Sánchez</td><td>Dominican Republic</td><td>47.76</td><td>Q, SB</td></tr> | |
| <tr><td>2</td><td>Jehue Gordon</td><td>Trinidad and Tobago</td><td>47.96</td><td>Q, NR</td></tr> | |
| <tr><td>3</td><td>Kerron Clement</td><td>United States</td><td>48.12</td><td>q, SB</td></tr> | |
| <tr><td>4</td><td>Dai Greene</td><td>Great Britain</td><td>48.19</td><td>q</td></tr> | |
| <tr><td>5</td><td>Mamadou Kasse Hanne</td><td>Senegal</td><td>48.80</td><td>PB</td></tr> | |
| <tr><td>6</td><td>Michael Bultheel</td><td>Belgium</td><td>49.10</td><td>PB</td></tr> | |
| <tr><td>7</td><td>Eric Alejandro</td><td>Puerto Rico</td><td>49.15</td><td>-</td></tr> | |
| <tr><td>8</td><td>Kurt Couto</td><td>Mozambique</td><td>51.55</td><td>-</td></tr> | |
| </table> | |