| Rank,Lane,Name,Nationality,Time,Notes | |
| -,4,Christine Ohuruogu,Great Britain & N.I. (GBR),49.41,NR | |
| -,5,Amantle Montsho,Botswana (BOT),49.41,- | |
| -,2,Stephanie McPherson,Jamaica (JAM),49.99,- | |
| 4,3,Natasha Hastings,United States (USA),50.30,- | |
| 5,6,Francena McCorory,United States (USA),50.68,- | |
| 6,7,Kseniya Ryzhova,Russia (RUS),50.98,- | |
| 7,1,Novlene Williams-Mills,Jamaica (JAM),51.49,- | |
| DQ,8,Antonina Krivoshapka,Russia (RUS),49.78,- | |