| Years tallest,Name,Image,Height ft / m,Floors,Year completed,Notes | |
| 1902–1909,Dyal-Upchurch Building,-,82 / 25,6,1902,Built after the Great Fire of 1901. The tallest building in Florida until 1909. | |
| 1909–1912,121 Atlantic Place,-,135 / 41,10,1909,The tallest building in Florida until 1912. | |
| 1912–1913,Florida Life Building,Florida Life Bldg.JPG,148 / 45,11,1912,Part of the Laura Street Trio. The tallest building in Florida until 1913. | |
| 1913–1926,Heard National Bank Building,-,180 / 55,15,1913,The tallest building in Florida until 1925. It is the only one of Jacksonville's and Florida's tallest buildings since 1902 to have been demolished. | |
| 1926–1954,Barnett National Bank Building,-,224 / 68,18,1926,- | |
| 1954–1967,Aetna Building,OnePrudentialPlazaJacksonville-Feb2010.JPG,309 / 94,22,1955,"Originally the Prudential Insurance Building. Aetna is slated to move to the suburbs in August, 2017." | |
| 1967–1974,Riverplace Tower,Riverplace.PNG,417 / 127,28,1967,Tallest building in Florida until 1972. | |
| 1974–1990,Wells Fargo Center,-,535 / 163,37,1974,"Formerly known as the Independent Life Building and the Modis Building. Tallest building in Florida until 1981, and the last of the tallest to be in Jacksonville." | |
| 1990–present,Bank of America Tower,-,617 / 188,42,1990,Jacksonville's current tallest building | |