| Rank Lane Name Nationality Time Notes | |
| - 4 Katinka Hosszú Hungary 4:26.36 WR | |
| - 3 Maya DiRado United States 4:31.15 - | |
| - 5 Mireia Belmonte Spain 4:32.39 - | |
| 4 6 Hannah Miley Great Britain 4:32.54 - | |
| 5 8 Emily Overholt Canada 4:34.70 - | |
| 6 7 Elizabeth Beisel United States 4:34.98 - | |
| 7 2 Aimee Willmott Great Britain 4:35.04 - | |
| 8 1 Sakiko Shimizu Japan 4:38.06 - | |