| Rank Lane Name Nationality Time Notes | |
| - 4 Inge de Bruijn Netherlands 24.32 - | |
| - 5 Therese Alshammar Sweden 24.51 - | |
| - 3 Dara Torres United States 24.63 AM | |
| 4 6 Amy Van Dyken United States 25.04 - | |
| 5 8 Martina Moravcová Slovakia 25.24 NR | |
| 6 2 Sandra Völker Germany 25.27 - | |
| 7 7 Alison Sheppard Great Britain 25.45 - | |
| 8 1 Sumika Minamoto Japan 25.65 - | |