| Rank,Heat,Lane,Name,Nationality,Time,Notes | |
| 1,3,4,Christian Coleman,United States (USA),9.97,Q | |
| 2,3,6,Usain Bolt,Jamaica (JAM),9.98,Q | |
| 3,2,4,Yohan Blake,Jamaica (JAM),10.04,Q | |
| 4,2,9,Reece Prescod,Great Britain & N.I. (GBR),10.05,Q | |
| 5,1,2,Akani Simbine,South Africa (RSA),10.05,Q | |
| 6,1,6,Justin Gatlin,United States (USA),10.09,Q | |
| 7,3,8,Jimmy Vicaut,France (FRA),10.09,q | |
| 8,2,6,Su Bingtian,China (CHN),10.10,q | |
| 9,3,7,Chijindu Ujah,Great Britain & N.I. (GBR),10.12,- | |
| 10,1,7,Ben Youssef Meïté,Ivory Coast (CIV),10.12,- | |
| 11,1,5,Julian Forte,Jamaica (JAM),10.13,- | |
| 12,2,7,Jak Ali Harvey,Turkey (TUR),10.16,- | |
| 13,2,8,Christopher Belcher,United States (USA),10.20,- | |
| 14,2,2,Emmanuel Matadi,Liberia (LBR),10.20,- | |
| 15,1,4,James Dasaolu,Great Britain & N.I. (GBR),10.22,- | |
| 16,1,3,Asuka Cambridge,Japan (JPN),10.25,- | |
| 17,3,2,Shuhei Tada,Japan (JPN),10.26,- | |
| 18,3,3,Emre Zafer Barnes,Turkey (TUR),10.27,- | |
| 19,2,5,Abdul Hakim Sani Brown,Japan (JPN),10.28,- | |
| 20,1,9,Xie Zhenye,China (CHN),10.28,- | |
| 21,2,3,Alex Wilson,Switzerland (SUI),10.30,- | |
| 22,3,5,Andrew Fisher,Bahrain (BHR),10.36,- | |
| 23,1,8,Kim Kuk-young,South Korea (KOR),10.40,- | |
| 24,3,9,Cejhae Greene,Antigua and Barbuda (ANT),10.64,- | |