| Rank,Lane,Name,Nationality,Time,Notes | |
| -,4,Inge de Bruijn,Netherlands,24.32,- | |
| -,5,Therese Alshammar,Sweden,24.51,- | |
| -,3,Dara Torres,United States,24.63,AM | |
| 4,6,Amy Van Dyken,United States,25.04,- | |
| 5,8,Martina Moravcová,Slovakia,25.24,NR | |
| 6,2,Sandra Völker,Germany,25.27,- | |
| 7,7,Alison Sheppard,Great Britain,25.45,- | |
| 8,1,Sumika Minamoto,Japan,25.65,- | |