| Rank Heat Lane Name Nationality Time Notes | |
| 1 3 4 Christian Coleman United States (USA) 9.97 Q | |
| 2 3 6 Usain Bolt Jamaica (JAM) 9.98 Q | |
| 3 2 4 Yohan Blake Jamaica (JAM) 10.04 Q | |
| 4 2 9 Reece Prescod Great Britain & N.I. (GBR) 10.05 Q | |
| 5 1 2 Akani Simbine South Africa (RSA) 10.05 Q | |
| 6 1 6 Justin Gatlin United States (USA) 10.09 Q | |
| 7 3 8 Jimmy Vicaut France (FRA) 10.09 q | |
| 8 2 6 Su Bingtian China (CHN) 10.10 q | |
| 9 3 7 Chijindu Ujah Great Britain & N.I. (GBR) 10.12 - | |
| 10 1 7 Ben Youssef Meïté Ivory Coast (CIV) 10.12 - | |
| 11 1 5 Julian Forte Jamaica (JAM) 10.13 - | |
| 12 2 7 Jak Ali Harvey Turkey (TUR) 10.16 - | |
| 13 2 8 Christopher Belcher United States (USA) 10.20 - | |
| 14 2 2 Emmanuel Matadi Liberia (LBR) 10.20 - | |
| 15 1 4 James Dasaolu Great Britain & N.I. (GBR) 10.22 - | |
| 16 1 3 Asuka Cambridge Japan (JPN) 10.25 - | |
| 17 3 2 Shuhei Tada Japan (JPN) 10.26 - | |
| 18 3 3 Emre Zafer Barnes Turkey (TUR) 10.27 - | |
| 19 2 5 Abdul Hakim Sani Brown Japan (JPN) 10.28 - | |
| 20 1 9 Xie Zhenye China (CHN) 10.28 - | |
| 21 2 3 Alex Wilson Switzerland (SUI) 10.30 - | |
| 22 3 5 Andrew Fisher Bahrain (BHR) 10.36 - | |
| 23 1 8 Kim Kuk-young South Korea (KOR) 10.40 - | |
| 24 3 9 Cejhae Greene Antigua and Barbuda (ANT) 10.64 - | |