| Rank Lane Name Nationality Time Notes | |
| - 4 Inge de Bruijn Netherlands 56.61 WR | |
| - 2 Martina Moravcová Slovakia 57.97 NR | |
| - 6 Dara Torres United States 58.20 - | |
| 4 5 Petria Thomas Australia 58.49 - | |
| 5 3 Jenny Thompson United States 58.73 - | |
| 6 7 Junko Onishi Japan 59.13 - | |
| 7 1 Susie O'Neill Australia 59.27 - | |
| 8 8 Diana Mocanu Romania 59.43 - | |