| Rank | Heat | Lane | Name | Nationality | Time | Notes |
| 1 | 3 | 4 | Christian Coleman | United States (USA) | 9.97 | Q |
| 2 | 3 | 6 | Usain Bolt | Jamaica (JAM) | 9.98 | Q |
| 3 | 2 | 4 | Yohan Blake | Jamaica (JAM) | 10.04 | Q |
| 4 | 2 | 9 | Reece Prescod | Great Britain & N.I. (GBR) | 10.05 | Q |
| 5 | 1 | 2 | Akani Simbine | South Africa (RSA) | 10.05 | Q |
| 6 | 1 | 6 | Justin Gatlin | United States (USA) | 10.09 | Q |
| 7 | 3 | 8 | Jimmy Vicaut | France (FRA) | 10.09 | q |
| 8 | 2 | 6 | Su Bingtian | China (CHN) | 10.10 | q |
| 9 | 3 | 7 | Chijindu Ujah | Great Britain & N.I. (GBR) | 10.12 | - |
| 10 | 1 | 7 | Ben Youssef Meïté | Ivory Coast (CIV) | 10.12 | - |
| 11 | 1 | 5 | Julian Forte | Jamaica (JAM) | 10.13 | - |
| 12 | 2 | 7 | Jak Ali Harvey | Turkey (TUR) | 10.16 | - |
| 13 | 2 | 8 | Christopher Belcher | United States (USA) | 10.20 | - |
| 14 | 2 | 2 | Emmanuel Matadi | Liberia (LBR) | 10.20 | - |
| 15 | 1 | 4 | James Dasaolu | Great Britain & N.I. (GBR) | 10.22 | - |
| 16 | 1 | 3 | Asuka Cambridge | Japan (JPN) | 10.25 | - |
| 17 | 3 | 2 | Shuhei Tada | Japan (JPN) | 10.26 | - |
| 18 | 3 | 3 | Emre Zafer Barnes | Turkey (TUR) | 10.27 | - |
| 19 | 2 | 5 | Abdul Hakim Sani Brown | Japan (JPN) | 10.28 | - |
| 20 | 1 | 9 | Xie Zhenye | China (CHN) | 10.28 | - |
| 21 | 2 | 3 | Alex Wilson | Switzerland (SUI) | 10.30 | - |
| 22 | 3 | 5 | Andrew Fisher | Bahrain (BHR) | 10.36 | - |
| 23 | 1 | 8 | Kim Kuk-young | South Korea (KOR) | 10.40 | - |
| 24 | 3 | 9 | Cejhae Greene | Antigua and Barbuda (ANT) | 10.64 | - |