| Rank | Lane | Name | Nationality | Time | Notes |
| - | 4 | Inge de Bruijn | Netherlands | 24.32 | - |
| - | 5 | Therese Alshammar | Sweden | 24.51 | - |
| - | 3 | Dara Torres | United States | 24.63 | AM |
| 4 | 6 | Amy Van Dyken | United States | 25.04 | - |
| 5 | 8 | Martina Moravcová | Slovakia | 25.24 | NR |
| 6 | 2 | Sandra Völker | Germany | 25.27 | - |
| 7 | 7 | Alison Sheppard | Great Britain | 25.45 | - |
| 8 | 1 | Sumika Minamoto | Japan | 25.65 | - |