| Rank | Lane | Name | Nationality | Time | Notes |
| - | 4 | Katinka Hosszú | Hungary | 4:26.36 | WR |
| - | 3 | Maya DiRado | United States | 4:31.15 | - |
| - | 5 | Mireia Belmonte | Spain | 4:32.39 | - |
| 4 | 6 | Hannah Miley | Great Britain | 4:32.54 | - |
| 5 | 8 | Emily Overholt | Canada | 4:34.70 | - |
| 6 | 7 | Elizabeth Beisel | United States | 4:34.98 | - |
| 7 | 2 | Aimee Willmott | Great Britain | 4:35.04 | - |
| 8 | 1 | Sakiko Shimizu | Japan | 4:38.06 | - |