| Rank | Lane | Name | Nationality | Time | Notes |
| - | 4 | Inge de Bruijn | Netherlands | 56.61 | WR |
| - | 2 | Martina Moravcová | Slovakia | 57.97 | NR |
| - | 6 | Dara Torres | United States | 58.20 | - |
| 4 | 5 | Petria Thomas | Australia | 58.49 | - |
| 5 | 3 | Jenny Thompson | United States | 58.73 | - |
| 6 | 7 | Junko Onishi | Japan | 59.13 | - |
| 7 | 1 | Susie O'Neill | Australia | 59.27 | - |
| 8 | 8 | Diana Mocanu | Romania | 59.43 | - |