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point,technique,Hormone testing,relationship/description/definition/use,Catecholamine testing,Drug monitoring...,Hormone testing...,factor/label/component/method/mechanism,Drug monitoring
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Migration rate dependency,Electrophoresis,,Directly proportional to rate of movement,,,,Charge of the molecule,
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Migration rate dependency,Electrophoresis,,Inversely proportional to rate of movement,,,,Size of the molecule,
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Migration rate dependency,Electrophoresis,,Increased current increases migration rate,,,,Electrical field,
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Migration rate dependency,Electrophoresis,,Increased ionic strength decreases migration rate,,,,Ionic strength of buffer,
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Migration rate dependency,Electrophoresis,,Decreased pH slows migration,,,,pH of buffer,
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Migration rate dependency,Electrophoresis,,Inversely proportional to migration rate,,,,Viscosity of supporting medium,
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Migration rate dependency,Electrophoresis,,High temperature can denature protein and slow migration,,,,System temperature,
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Analytic electrophoretic procedures,Electrophoresis,,Proteins are amphoteric...,,,,Protein electrophoresis,
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Analytic electrophoretic procedures,Electrophoresis,,Similar to protein electrophoresis...,,,,Isoenzyme electrophoresis,
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Components,Immunoassay,,A substance that can elicit an immune response...,,,,Antigen,
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Components,Immunoassay,,An immunoglobulin formed in response to a foreign substance...,,,,Antibody,
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Immunochemical labels,Immunoassay,,"Attached to the antibody, allowing colorimetric quantitation...",,,,Enzyme labels,
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Immunochemical labels,Immunoassay,,"Emit photons when excited, used in fluorescence-based detection...",,,,Fluorescent labels,
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Immunochemical labels,Immunoassay,,Release energy in the form of visible light upon returning to ground state...,,,,Chemiluminescent labels,
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Immunochemical labels,Immunoassay,,"Emit radiation (gamma rays) upon decay, detected for quantitation...",,,,Radioisotope labels,
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Methodologies,Immunoassay,,Enzyme-based,,,,ELISA,
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Methodologies,Immunoassay,,Enzyme-based,,,,EIA/EMIT,
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Methodologies,Immunoassay,,Fluorescence-based,,,,FPIA,
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Methodologies,Immunoassay,,Fluorescence-based,,,,FIA,
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Methodologies,Immunoassay,,Radiation-based,,,,RIA,
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Methodologies,Immunoassay,,Radiation-based,,,,IRMA,
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Mechanisms of separation,Chromatography,,Based on the competition between the sample and the mobile phase for binding sites...,,,,Adsorption chromatography,
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Mechanisms of separation,Chromatography,,Depends on the solubility of the solute in nonpolar or polar solvents...,,,,Partition chromatography,
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Mechanisms of separation,Chromatography,,Involves separation of solutes by their size and charge of the ionic species present...,,,,Ion-exchange chromatography,
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Chromatographic procedures,Chromatography,,Semi-quantitative screening test...,,,,Thin-layer chromatography (TLC),
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Chromatographic procedures,Chromatography,,Provides quantitative results...,,,,High-performance liquid chromatography (HPLC),
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