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| "text": [ | |
| "GENETICALLY ENGINEERED V79 CELLS", | |
| "FOR TOXICITY AND DRUG METABOLISM STUDIES", | |
| "Johannes Doehmer, Franz Oesch, Catherine Janssens, Michael", | |
| "Edigkaufer, Martina Barrenscheen, Karl Ludwig Platt,", | |
| "Albrecht Seidel and Hansruedi Glatt, Institut für", | |
| "Toxikologie, Universität Mainz, Obere Zahlbacher Str 67,", | |
| "D-6500 Mainz, Germany", | |
| "Mammalian cells are being genetically engineered for", | |
| "stable expression of xenobiotics metabolizing enzymes.", | |
| "This approach marks a fundamental and new development in", | |
| "the field of in vitro biotransformation models.", | |
| "This laboratory concentrates on the development of V79", | |
| "Chinese hamster derived cells genetically engineered for", | |
| "stable expression of cytochromes P450. V79 cells have been", | |
| "chosen to serve as recipient cell in cDNA mediated gene", | |
| "transfer for various reasons. V79 cells have been the", | |
| "preferred indicator cells in mutagenicity and cytotoxicity", | |
| "studies for almost twenty years. They are extremely well", | |
| "adapted to culture conditions, as they are among the", | |
| "fastest growing cell lines with an avarage doubling time", | |
| "of 12 hours, and as their karyotype is very stable.", | |
| "However, V79 Chinese hamster cells lack cytochrome P450", | |
| "activity. As there is no endogenous P450 background", | |
| "activity, genetically engineered V79 cells are highly", | |
| "defined for one particular P450 isoform. This has already", | |
| "been demonstrated for rat cytochromes P450IIB1 (1),", | |
| "P450IA1 (2), and P450IA2 (3) in metabolism-, mutagenicity,", | |
| "and cytotoxicity-studies. Recently, V79 cells were", | |
| "succesfully transfected with human cytochromes P450IA2 and", | |
| "IIE1 CDNA.", | |
| "References", | |
| "(1) : Doehmer et al., PNAS, vol.85,5769-5773 1988;", | |
| "(2) : Dogra et al.,Mol Pharmacol, vol.37, 608-613, 1990;", | |
| "(3) : Janssens et al., submitted;", | |
| "Supported by Deutsche Forschungsgemeinschaft (SFB302) and", | |
| "Bundesgesundheitsamt (ZEBET)", | |
| "Source: https://www.industrydocuments.ucsf.edu/docs/hrpp0144" | |
| ] | |
| } | |
| ] | |
| } |