| | |
| | |
| |
|
| | """ Read SPE files. |
| | |
| | This plugin supports reading files saved in the Princeton Instruments |
| | SPE file format. |
| | |
| | Parameters |
| | ---------- |
| | check_filesize : bool |
| | The number of frames in the file is stored in the file header. However, |
| | this number may be wrong for certain software. If this is `True` |
| | (default), derive the number of frames also from the file size and |
| | raise a warning if the two values do not match. |
| | char_encoding : str |
| | Deprecated. Exists for backwards compatibility; use ``char_encoding`` of |
| | ``metadata`` instead. |
| | sdt_meta : bool |
| | Deprecated. Exists for backwards compatibility; use ``sdt_control`` of |
| | ``metadata`` instead. |
| | |
| | Methods |
| | ------- |
| | .. note:: |
| | Check the respective function for a list of supported kwargs and detailed |
| | documentation. |
| | |
| | .. autosummary:: |
| | :toctree: |
| | |
| | SpePlugin.read |
| | SpePlugin.iter |
| | SpePlugin.properties |
| | SpePlugin.metadata |
| | |
| | """ |
| |
|
| | from datetime import datetime |
| | import logging |
| | import os |
| | from typing import ( |
| | Any, |
| | Callable, |
| | Dict, |
| | Iterator, |
| | List, |
| | Mapping, |
| | Optional, |
| | Sequence, |
| | Tuple, |
| | Union, |
| | ) |
| | import warnings |
| |
|
| | import numpy as np |
| |
|
| | from ..core.request import Request, IOMode, InitializationError |
| | from ..core.v3_plugin_api import PluginV3, ImageProperties |
| |
|
| |
|
| | logger = logging.getLogger(__name__) |
| |
|
| |
|
| | class Spec: |
| | """SPE file specification data |
| | |
| | Tuples of (offset, datatype, count), where offset is the offset in the SPE |
| | file and datatype is the datatype as used in `numpy.fromfile`() |
| | |
| | `data_start` is the offset of actual image data. |
| | |
| | `dtypes` translates SPE datatypes (0...4) to numpy ones, e. g. dtypes[0] |
| | is dtype("<f") (which is np.float32). |
| | |
| | `controllers` maps the `type` metadata to a human readable name |
| | |
| | `readout_modes` maps the `readoutMode` metadata to something human readable |
| | although this may not be accurate since there is next to no documentation |
| | to be found. |
| | """ |
| |
|
| | basic = { |
| | "datatype": (108, "<h"), |
| | "xdim": (42, "<H"), |
| | "ydim": (656, "<H"), |
| | "xml_footer_offset": (678, "<Q"), |
| | "NumFrames": (1446, "<i"), |
| | "file_header_ver": (1992, "<f"), |
| | } |
| |
|
| | metadata = { |
| | |
| | "NumROI": (1510, "<h"), |
| | "ROIs": ( |
| | 1512, |
| | np.dtype( |
| | [ |
| | ("startx", "<H"), |
| | ("endx", "<H"), |
| | ("groupx", "<H"), |
| | ("starty", "<H"), |
| | ("endy", "<H"), |
| | ("groupy", "<H"), |
| | ] |
| | ), |
| | 10, |
| | ), |
| | |
| | "xDimDet": (6, "<H"), |
| | "yDimDet": (18, "<H"), |
| | "VChipXdim": (14, "<h"), |
| | "VChipYdim": (16, "<h"), |
| | |
| | "controller_version": (0, "<h"), |
| | "logic_output": (2, "<h"), |
| | "amp_high_cap_low_noise": (4, "<H"), |
| | "mode": (8, "<h"), |
| | "exposure_sec": (10, "<f"), |
| | "date": (20, "<10S"), |
| | "detector_temp": (36, "<f"), |
| | "detector_type": (40, "<h"), |
| | "st_diode": (44, "<h"), |
| | "delay_time": (46, "<f"), |
| | |
| | |
| | "shutter_control": (50, "<H"), |
| | "absorb_live": (52, "<h"), |
| | "absorb_mode": (54, "<H"), |
| | "can_do_virtual_chip": (56, "<h"), |
| | "threshold_min_live": (58, "<h"), |
| | "threshold_min_val": (60, "<f"), |
| | "threshold_max_live": (64, "<h"), |
| | "threshold_max_val": (66, "<f"), |
| | "time_local": (172, "<7S"), |
| | "time_utc": (179, "<7S"), |
| | "adc_offset": (188, "<H"), |
| | "adc_rate": (190, "<H"), |
| | "adc_type": (192, "<H"), |
| | "adc_resolution": (194, "<H"), |
| | "adc_bit_adjust": (196, "<H"), |
| | "gain": (198, "<H"), |
| | "comments": (200, "<80S", 5), |
| | "geometric": (600, "<H"), |
| | "sw_version": (688, "<16S"), |
| | "spare_4": (742, "<436S"), |
| | "XPrePixels": (98, "<h"), |
| | "XPostPixels": (100, "<h"), |
| | "YPrePixels": (102, "<h"), |
| | "YPostPixels": (104, "<h"), |
| | "readout_time": (672, "<f"), |
| | "xml_footer_offset": (678, "<Q"), |
| | "type": (704, "<h"), |
| | "clockspeed_us": (1428, "<f"), |
| | "readout_mode": (1480, "<H"), |
| | "window_size": (1482, "<H"), |
| | "file_header_ver": (1992, "<f"), |
| | } |
| |
|
| | data_start = 4100 |
| |
|
| | dtypes = { |
| | 0: np.dtype(np.float32), |
| | 1: np.dtype(np.int32), |
| | 2: np.dtype(np.int16), |
| | 3: np.dtype(np.uint16), |
| | 8: np.dtype(np.uint32), |
| | } |
| |
|
| | controllers = [ |
| | "new120 (Type II)", |
| | "old120 (Type I)", |
| | "ST130", |
| | "ST121", |
| | "ST138", |
| | "DC131 (PentaMax)", |
| | "ST133 (MicroMax/Roper)", |
| | "ST135 (GPIB)", |
| | "VTCCD", |
| | "ST116 (GPIB)", |
| | "OMA3 (GPIB)", |
| | "OMA4", |
| | ] |
| |
|
| | |
| | |
| | readout_modes = ["full frame", "frame transfer", "kinetics"] |
| |
|
| | |
| | |
| | no_decode = ["spare_4"] |
| |
|
| |
|
| | class SDTControlSpec: |
| | """Extract metadata written by the SDT-control software |
| | |
| | Some of it is encoded in the comment strings |
| | (see :py:meth:`parse_comments`). Also, date and time are encoded in a |
| | peculiar way (see :py:meth:`get_datetime`). Use :py:meth:`extract_metadata` |
| | to update the metadata dict. |
| | """ |
| |
|
| | months = { |
| | |
| | "Jän": 1, |
| | "Jan": 1, |
| | "Feb": 2, |
| | "Mär": 3, |
| | "Mar": 3, |
| | "Apr": 4, |
| | "Mai": 5, |
| | "May": 5, |
| | "Jun": 6, |
| | "Jul": 7, |
| | "Aug": 8, |
| | "Sep": 9, |
| | "Okt": 10, |
| | "Oct": 10, |
| | "Nov": 11, |
| | "Dez": 12, |
| | "Dec": 12, |
| | } |
| |
|
| | sequence_types = { |
| | |
| | "SEQU": "standard", |
| | "SETO": "TOCCSL", |
| | "KINE": "kinetics", |
| | "SEAR": "arbitrary", |
| | } |
| |
|
| | class CommentDesc: |
| | """Describe how to extract a metadata entry from a comment string""" |
| |
|
| | n: int |
| | """Which of the 5 SPE comment fields to use.""" |
| | slice: slice |
| | """Which characters from the `n`-th comment to use.""" |
| | cvt: Callable[[str], Any] |
| | """How to convert characters to something useful.""" |
| | scale: Union[None, float] |
| | """Optional scaling factor for numbers""" |
| |
|
| | def __init__( |
| | self, |
| | n: int, |
| | slice: slice, |
| | cvt: Callable[[str], Any] = str, |
| | scale: Optional[float] = None, |
| | ): |
| | self.n = n |
| | self.slice = slice |
| | self.cvt = cvt |
| | self.scale = scale |
| |
|
| | comment_fields = { |
| | (5, 0): { |
| | "sdt_major_version": CommentDesc(4, slice(66, 68), int), |
| | "sdt_minor_version": CommentDesc(4, slice(68, 70), int), |
| | "sdt_controller_name": CommentDesc(4, slice(0, 6), str), |
| | "exposure_time": CommentDesc(1, slice(64, 73), float, 10**-6), |
| | "color_code": CommentDesc(4, slice(10, 14), str), |
| | "detection_channels": CommentDesc(4, slice(15, 16), int), |
| | "background_subtraction": CommentDesc(4, 14, lambda x: x == "B"), |
| | "em_active": CommentDesc(4, 32, lambda x: x == "E"), |
| | "em_gain": CommentDesc(4, slice(28, 32), int), |
| | "modulation_active": CommentDesc(4, 33, lambda x: x == "A"), |
| | "pixel_size": CommentDesc(4, slice(25, 28), float, 0.1), |
| | "sequence_type": CommentDesc( |
| | 4, slice(6, 10), lambda x: __class__.sequence_types[x] |
| | ), |
| | "grid": CommentDesc(4, slice(16, 25), float, 10**-6), |
| | "n_macro": CommentDesc(1, slice(0, 4), int), |
| | "delay_macro": CommentDesc(1, slice(10, 19), float, 10**-3), |
| | "n_mini": CommentDesc(1, slice(4, 7), int), |
| | "delay_mini": CommentDesc(1, slice(19, 28), float, 10**-6), |
| | "n_micro": CommentDesc(1, slice(7, 10), int), |
| | "delay_micro": CommentDesc(1, slice(28, 37), float, 10**-6), |
| | "n_subpics": CommentDesc(1, slice(7, 10), int), |
| | "delay_shutter": CommentDesc(1, slice(73, 79), float, 10**-6), |
| | "delay_prebleach": CommentDesc(1, slice(37, 46), float, 10**-6), |
| | "bleach_time": CommentDesc(1, slice(46, 55), float, 10**-6), |
| | "recovery_time": CommentDesc(1, slice(55, 64), float, 10**-6), |
| | }, |
| | (5, 1): { |
| | "bleach_piezo_active": CommentDesc(4, slice(34, 35), lambda x: x == "z") |
| | }, |
| | } |
| |
|
| | @staticmethod |
| | def get_comment_version(comments: Sequence[str]) -> Tuple[int, int]: |
| | """Get the version of SDT-control metadata encoded in the comments |
| | |
| | Parameters |
| | ---------- |
| | comments |
| | List of SPE file comments, typically ``metadata["comments"]``. |
| | |
| | Returns |
| | ------- |
| | Major and minor version. ``-1, -1`` if detection failed. |
| | """ |
| | if comments[4][70:76] != "COMVER": |
| | return -1, -1 |
| | try: |
| | return int(comments[4][76:78]), int(comments[4][78:80]) |
| | except ValueError: |
| | return -1, -1 |
| |
|
| | @staticmethod |
| | def parse_comments( |
| | comments: Sequence[str], version: Tuple[int, int] |
| | ) -> Dict[str, Any]: |
| | """Extract SDT-control metadata from comments |
| | |
| | Parameters |
| | ---------- |
| | comments |
| | List of SPE file comments, typically ``metadata["comments"]``. |
| | version |
| | Major and minor version of SDT-control metadata format |
| | |
| | Returns |
| | ------- |
| | Dict of metadata |
| | """ |
| | sdt_md = {} |
| | for minor in range(version[1] + 1): |
| | |
| | |
| | |
| | |
| | try: |
| | cmt = __class__.comment_fields[version[0], minor] |
| | except KeyError: |
| | continue |
| | for name, spec in cmt.items(): |
| | try: |
| | v = spec.cvt(comments[spec.n][spec.slice]) |
| | if spec.scale is not None: |
| | v *= spec.scale |
| | sdt_md[name] = v |
| | except Exception as e: |
| | warnings.warn( |
| | f"Failed to decode SDT-control metadata field `{name}`: {e}" |
| | ) |
| | sdt_md[name] = None |
| | if version not in __class__.comment_fields: |
| | supported_ver = ", ".join( |
| | map(lambda x: f"{x[0]}.{x[1]:02}", __class__.comment_fields) |
| | ) |
| | warnings.warn( |
| | f"Unsupported SDT-control metadata version {version[0]}.{version[1]:02}. " |
| | f"Only versions {supported_ver} are supported. " |
| | "Some or all SDT-control metadata may be missing." |
| | ) |
| | comment = comments[0] + comments[2] |
| | sdt_md["comment"] = comment.strip() |
| | return sdt_md |
| |
|
| | @staticmethod |
| | def get_datetime(date: str, time: str) -> Union[datetime, None]: |
| | """Turn date and time saved by SDT-control into proper datetime object |
| | |
| | Parameters |
| | ---------- |
| | date |
| | SPE file date, typically ``metadata["date"]``. |
| | time |
| | SPE file date, typically ``metadata["time_local"]``. |
| | |
| | Returns |
| | ------- |
| | File's datetime if parsing was succsessful, else None. |
| | """ |
| | try: |
| | month = __class__.months[date[2:5]] |
| | return datetime( |
| | int(date[5:9]), |
| | month, |
| | int(date[0:2]), |
| | int(time[0:2]), |
| | int(time[2:4]), |
| | int(time[4:6]), |
| | ) |
| | except Exception as e: |
| | logger.info(f"Failed to decode date from SDT-control metadata: {e}.") |
| |
|
| | @staticmethod |
| | def extract_metadata(meta: Mapping, char_encoding: str = "latin1"): |
| | """Extract SDT-control metadata from SPE metadata |
| | |
| | SDT-control stores some metadata in comments and other fields. |
| | Extract them and remove unused entries. |
| | |
| | Parameters |
| | ---------- |
| | meta |
| | SPE file metadata. Modified in place. |
| | char_encoding |
| | Character encoding used to decode strings in the metadata. |
| | """ |
| | comver = __class__.get_comment_version(meta["comments"]) |
| | if any(c < 0 for c in comver): |
| | |
| | logger.debug("SDT-control comments not found.") |
| | return |
| |
|
| | sdt_meta = __class__.parse_comments(meta["comments"], comver) |
| | meta.pop("comments") |
| | meta.update(sdt_meta) |
| |
|
| | |
| | dt = __class__.get_datetime(meta["date"], meta["time_local"]) |
| | if dt: |
| | meta["datetime"] = dt |
| | meta.pop("date") |
| | meta.pop("time_local") |
| |
|
| | sp4 = meta["spare_4"] |
| | try: |
| | meta["modulation_script"] = sp4.decode(char_encoding) |
| | meta.pop("spare_4") |
| | except UnicodeDecodeError: |
| | warnings.warn( |
| | "Failed to decode SDT-control laser " |
| | "modulation script. Bad char_encoding?" |
| | ) |
| |
|
| | |
| | meta.pop("time_utc") |
| | meta.pop("exposure_sec") |
| |
|
| |
|
| | class SpePlugin(PluginV3): |
| | def __init__( |
| | self, |
| | request: Request, |
| | check_filesize: bool = True, |
| | char_encoding: Optional[str] = None, |
| | sdt_meta: Optional[bool] = None, |
| | ) -> None: |
| | """Instantiate a new SPE file plugin object |
| | |
| | Parameters |
| | ---------- |
| | request : Request |
| | A request object representing the resource to be operated on. |
| | check_filesize : bool |
| | If True, compute the number of frames from the filesize, compare it |
| | to the frame count in the file header, and raise a warning if the |
| | counts don't match. (Certain software may create files with |
| | char_encoding : str |
| | Deprecated. Exists for backwards compatibility; use ``char_encoding`` of |
| | ``metadata`` instead. |
| | sdt_meta : bool |
| | Deprecated. Exists for backwards compatibility; use ``sdt_control`` of |
| | ``metadata`` instead. |
| | |
| | """ |
| |
|
| | super().__init__(request) |
| | if request.mode.io_mode == IOMode.write: |
| | raise InitializationError("cannot write SPE files") |
| |
|
| | if char_encoding is not None: |
| | warnings.warn( |
| | "Passing `char_encoding` to the constructor is deprecated. " |
| | "Use `char_encoding` parameter of the `metadata()` method " |
| | "instead.", |
| | DeprecationWarning, |
| | ) |
| | self._char_encoding = char_encoding |
| | if sdt_meta is not None: |
| | warnings.warn( |
| | "Passing `sdt_meta` to the constructor is deprecated. " |
| | "Use `sdt_control` parameter of the `metadata()` method " |
| | "instead.", |
| | DeprecationWarning, |
| | ) |
| | self._sdt_meta = sdt_meta |
| |
|
| | self._file = self.request.get_file() |
| |
|
| | try: |
| | |
| | |
| | info = self._parse_header(Spec.basic, "latin1") |
| | self._file_header_ver = info["file_header_ver"] |
| | self._dtype = Spec.dtypes[info["datatype"]] |
| | self._shape = (info["ydim"], info["xdim"]) |
| | self._len = info["NumFrames"] |
| |
|
| | if check_filesize: |
| | |
| | |
| | |
| | |
| | if info["file_header_ver"] >= 3: |
| | data_end = info["xml_footer_offset"] |
| | else: |
| | self._file.seek(0, os.SEEK_END) |
| | data_end = self._file.tell() |
| | line = data_end - Spec.data_start |
| | line //= self._shape[0] * self._shape[1] * self._dtype.itemsize |
| | if line != self._len: |
| | warnings.warn( |
| | f"The file header of {self.request.filename} claims there are " |
| | f"{self._len} frames, but there are actually {line} frames." |
| | ) |
| | self._len = min(line, self._len) |
| | self._file.seek(Spec.data_start) |
| | except Exception: |
| | raise InitializationError("SPE plugin cannot read the provided file.") |
| |
|
| | def read(self, *, index: int = ...) -> np.ndarray: |
| | """Read a frame or all frames from the file |
| | |
| | Parameters |
| | ---------- |
| | index : int |
| | Select the index-th frame from the file. If index is `...`, |
| | select all frames and stack them along a new axis. |
| | |
| | Returns |
| | ------- |
| | A Numpy array of pixel values. |
| | |
| | """ |
| |
|
| | if index is Ellipsis: |
| | read_offset = Spec.data_start |
| | count = self._shape[0] * self._shape[1] * self._len |
| | out_shape = (self._len, *self._shape) |
| | elif index < 0: |
| | raise IndexError(f"Index `{index}` is smaller than 0.") |
| | elif index >= self._len: |
| | raise IndexError( |
| | f"Index `{index}` exceeds the number of frames stored in this file (`{self._len}`)." |
| | ) |
| | else: |
| | read_offset = ( |
| | Spec.data_start |
| | + index * self._shape[0] * self._shape[1] * self._dtype.itemsize |
| | ) |
| | count = self._shape[0] * self._shape[1] |
| | out_shape = self._shape |
| |
|
| | self._file.seek(read_offset) |
| | data = np.fromfile(self._file, dtype=self._dtype, count=count) |
| | return data.reshape(out_shape) |
| |
|
| | def iter(self) -> Iterator[np.ndarray]: |
| | """Iterate over the frames in the file |
| | |
| | Yields |
| | ------ |
| | A Numpy array of pixel values. |
| | """ |
| |
|
| | return (self.read(index=i) for i in range(self._len)) |
| |
|
| | def metadata( |
| | self, |
| | index: int = ..., |
| | exclude_applied: bool = True, |
| | char_encoding: str = "latin1", |
| | sdt_control: bool = True, |
| | ) -> Dict[str, Any]: |
| | """SPE specific metadata. |
| | |
| | Parameters |
| | ---------- |
| | index : int |
| | Ignored as SPE files only store global metadata. |
| | exclude_applied : bool |
| | Ignored. Exists for API compatibility. |
| | char_encoding : str |
| | The encoding to use when parsing strings. |
| | sdt_control : bool |
| | If `True`, decode special metadata written by the |
| | SDT-control software if present. |
| | |
| | Returns |
| | ------- |
| | metadata : dict |
| | Key-value pairs of metadata. |
| | |
| | Notes |
| | ----- |
| | SPE v3 stores metadata as XML, whereas SPE v2 uses a binary format. |
| | |
| | .. rubric:: Supported SPE v2 Metadata fields |
| | |
| | ROIs : list of dict |
| | Regions of interest used for recording images. Each dict has the |
| | "top_left" key containing x and y coordinates of the top left corner, |
| | the "bottom_right" key with x and y coordinates of the bottom right |
| | corner, and the "bin" key with number of binned pixels in x and y |
| | directions. |
| | comments : list of str |
| | The SPE format allows for 5 comment strings of 80 characters each. |
| | controller_version : int |
| | Hardware version |
| | logic_output : int |
| | Definition of output BNC |
| | amp_hi_cap_low_noise : int |
| | Amp switching mode |
| | mode : int |
| | Timing mode |
| | exp_sec : float |
| | Alternative exposure in seconds |
| | date : str |
| | Date string |
| | detector_temp : float |
| | Detector temperature |
| | detector_type : int |
| | CCD / diode array type |
| | st_diode : int |
| | Trigger diode |
| | delay_time : float |
| | Used with async mode |
| | shutter_control : int |
| | Normal, disabled open, or disabled closed |
| | absorb_live : bool |
| | on / off |
| | absorb_mode : int |
| | Reference strip or file |
| | can_do_virtual_chip : bool |
| | True or False whether chip can do virtual chip |
| | threshold_min_live : bool |
| | on / off |
| | threshold_min_val : float |
| | Threshold minimum value |
| | threshold_max_live : bool |
| | on / off |
| | threshold_max_val : float |
| | Threshold maximum value |
| | time_local : str |
| | Experiment local time |
| | time_utc : str |
| | Experiment UTC time |
| | adc_offset : int |
| | ADC offset |
| | adc_rate : int |
| | ADC rate |
| | adc_type : int |
| | ADC type |
| | adc_resolution : int |
| | ADC resolution |
| | adc_bit_adjust : int |
| | ADC bit adjust |
| | gain : int |
| | gain |
| | sw_version : str |
| | Version of software which created this file |
| | spare_4 : bytes |
| | Reserved space |
| | readout_time : float |
| | Experiment readout time |
| | type : str |
| | Controller type |
| | clockspeed_us : float |
| | Vertical clock speed in microseconds |
| | readout_mode : ["full frame", "frame transfer", "kinetics", ""] |
| | Readout mode. Empty string means that this was not set by the |
| | Software. |
| | window_size : int |
| | Window size for Kinetics mode |
| | file_header_ver : float |
| | File header version |
| | chip_size : [int, int] |
| | x and y dimensions of the camera chip |
| | virt_chip_size : [int, int] |
| | Virtual chip x and y dimensions |
| | pre_pixels : [int, int] |
| | Pre pixels in x and y dimensions |
| | post_pixels : [int, int], |
| | Post pixels in x and y dimensions |
| | geometric : list of {"rotate", "reverse", "flip"} |
| | Geometric operations |
| | sdt_major_version : int |
| | (only for files created by SDT-control) |
| | Major version of SDT-control software |
| | sdt_minor_version : int |
| | (only for files created by SDT-control) |
| | Minor version of SDT-control software |
| | sdt_controller_name : str |
| | (only for files created by SDT-control) |
| | Controller name |
| | exposure_time : float |
| | (only for files created by SDT-control) |
| | Exposure time in seconds |
| | color_code : str |
| | (only for files created by SDT-control) |
| | Color channels used |
| | detection_channels : int |
| | (only for files created by SDT-control) |
| | Number of channels |
| | background_subtraction : bool |
| | (only for files created by SDT-control) |
| | Whether background subtraction war turned on |
| | em_active : bool |
| | (only for files created by SDT-control) |
| | Whether EM was turned on |
| | em_gain : int |
| | (only for files created by SDT-control) |
| | EM gain |
| | modulation_active : bool |
| | (only for files created by SDT-control) |
| | Whether laser modulation (“attenuate”) was turned on |
| | pixel_size : float |
| | (only for files created by SDT-control) |
| | Camera pixel size |
| | sequence_type : str |
| | (only for files created by SDT-control) |
| | Type of sequnce (standard, TOCCSL, arbitrary, …) |
| | grid : float |
| | (only for files created by SDT-control) |
| | Sequence time unit (“grid size”) in seconds |
| | n_macro : int |
| | (only for files created by SDT-control) |
| | Number of macro loops |
| | delay_macro : float |
| | (only for files created by SDT-control) |
| | Time between macro loops in seconds |
| | n_mini : int |
| | (only for files created by SDT-control) |
| | Number of mini loops |
| | delay_mini : float |
| | (only for files created by SDT-control) |
| | Time between mini loops in seconds |
| | n_micro : int (only for files created by SDT-control) |
| | Number of micro loops |
| | delay_micro : float (only for files created by SDT-control) |
| | Time between micro loops in seconds |
| | n_subpics : int |
| | (only for files created by SDT-control) |
| | Number of sub-pictures |
| | delay_shutter : float |
| | (only for files created by SDT-control) |
| | Camera shutter delay in seconds |
| | delay_prebleach : float |
| | (only for files created by SDT-control) |
| | Pre-bleach delay in seconds |
| | bleach_time : float |
| | (only for files created by SDT-control) |
| | Bleaching time in seconds |
| | recovery_time : float |
| | (only for files created by SDT-control) |
| | Recovery time in seconds |
| | comment : str |
| | (only for files created by SDT-control) |
| | User-entered comment. This replaces the "comments" field. |
| | datetime : datetime.datetime |
| | (only for files created by SDT-control) |
| | Combines the "date" and "time_local" keys. The latter two plus |
| | "time_utc" are removed. |
| | modulation_script : str |
| | (only for files created by SDT-control) |
| | Laser modulation script. Replaces the "spare_4" key. |
| | bleach_piezo_active : bool |
| | (only for files created by SDT-control) |
| | Whether piezo for bleaching was enabled |
| | """ |
| |
|
| | if self._file_header_ver < 3: |
| | if self._char_encoding is not None: |
| | char_encoding = self._char_encoding |
| | if self._sdt_meta is not None: |
| | sdt_control = self._sdt_meta |
| | return self._metadata_pre_v3(char_encoding, sdt_control) |
| | return self._metadata_post_v3() |
| |
|
| | def _metadata_pre_v3(self, char_encoding: str, sdt_control: bool) -> Dict[str, Any]: |
| | """Extract metadata from SPE v2 files |
| | |
| | Parameters |
| | ---------- |
| | char_encoding |
| | String character encoding |
| | sdt_control |
| | If `True`, try to decode special metadata written by the |
| | SDT-control software. |
| | |
| | Returns |
| | ------- |
| | dict mapping metadata names to values. |
| | |
| | """ |
| |
|
| | m = self._parse_header(Spec.metadata, char_encoding) |
| |
|
| | nr = m.pop("NumROI", None) |
| | nr = 1 if nr < 1 else nr |
| | m["ROIs"] = roi_array_to_dict(m["ROIs"][:nr]) |
| |
|
| | |
| | m["chip_size"] = [m.pop(k, None) for k in ("xDimDet", "yDimDet")] |
| | m["virt_chip_size"] = [m.pop(k, None) for k in ("VChipXdim", "VChipYdim")] |
| | m["pre_pixels"] = [m.pop(k, None) for k in ("XPrePixels", "YPrePixels")] |
| | m["post_pixels"] = [m.pop(k, None) for k in ("XPostPixels", "YPostPixels")] |
| |
|
| | |
| | m["comments"] = [str(c) for c in m["comments"]] |
| |
|
| | |
| | g = [] |
| | f = m.pop("geometric", 0) |
| | if f & 1: |
| | g.append("rotate") |
| | if f & 2: |
| | g.append("reverse") |
| | if f & 4: |
| | g.append("flip") |
| | m["geometric"] = g |
| |
|
| | |
| | t = m["type"] |
| | if 1 <= t <= len(Spec.controllers): |
| | m["type"] = Spec.controllers[t - 1] |
| | else: |
| | m["type"] = None |
| | r = m["readout_mode"] |
| | if 1 <= r <= len(Spec.readout_modes): |
| | m["readout_mode"] = Spec.readout_modes[r - 1] |
| | else: |
| | m["readout_mode"] = None |
| |
|
| | |
| | for k in ( |
| | "absorb_live", |
| | "can_do_virtual_chip", |
| | "threshold_min_live", |
| | "threshold_max_live", |
| | ): |
| | m[k] = bool(m[k]) |
| |
|
| | |
| | if sdt_control: |
| | SDTControlSpec.extract_metadata(m, char_encoding) |
| |
|
| | return m |
| |
|
| | def _metadata_post_v3(self) -> Dict[str, Any]: |
| | """Extract XML metadata from SPE v3 files |
| | |
| | Returns |
| | ------- |
| | dict with key `"__xml"`, whose value is the XML metadata |
| | """ |
| |
|
| | info = self._parse_header(Spec.basic, "latin1") |
| | self._file.seek(info["xml_footer_offset"]) |
| | xml = self._file.read() |
| | return {"__xml": xml} |
| |
|
| | def properties(self, index: int = ...) -> ImageProperties: |
| | """Standardized ndimage metadata. |
| | |
| | Parameters |
| | ---------- |
| | index : int |
| | If the index is an integer, select the index-th frame and return |
| | its properties. If index is an Ellipsis (...), return the |
| | properties of all frames in the file stacked along a new batch |
| | dimension. |
| | |
| | Returns |
| | ------- |
| | properties : ImageProperties |
| | A dataclass filled with standardized image metadata. |
| | """ |
| |
|
| | if index is Ellipsis: |
| | return ImageProperties( |
| | shape=(self._len, *self._shape), |
| | dtype=self._dtype, |
| | n_images=self._len, |
| | is_batch=True, |
| | ) |
| | return ImageProperties(shape=self._shape, dtype=self._dtype, is_batch=False) |
| |
|
| | def _parse_header( |
| | self, spec: Mapping[str, Tuple], char_encoding: str |
| | ) -> Dict[str, Any]: |
| | """Get information from SPE file header |
| | |
| | Parameters |
| | ---------- |
| | spec |
| | Maps header entry name to its location, data type description and |
| | optionally number of entries. See :py:attr:`Spec.basic` and |
| | :py:attr:`Spec.metadata`. |
| | char_encoding |
| | String character encoding |
| | |
| | Returns |
| | ------- |
| | Dict mapping header entry name to its value |
| | """ |
| |
|
| | ret = {} |
| | |
| | decode = np.vectorize(lambda x: x.decode(char_encoding)) |
| |
|
| | for name, sp in spec.items(): |
| | self._file.seek(sp[0]) |
| | cnt = 1 if len(sp) < 3 else sp[2] |
| | v = np.fromfile(self._file, dtype=sp[1], count=cnt) |
| | if v.dtype.kind == "S" and name not in Spec.no_decode: |
| | |
| | try: |
| | v = decode(v) |
| | except Exception: |
| | warnings.warn( |
| | f'Failed to decode "{name}" metadata ' |
| | "string. Check `char_encoding` parameter." |
| | ) |
| |
|
| | try: |
| | |
| | |
| | v = v.item() |
| | except ValueError: |
| | v = np.squeeze(v) |
| | ret[name] = v |
| | return ret |
| |
|
| |
|
| | def roi_array_to_dict(a: np.ndarray) -> List[Dict[str, List[int]]]: |
| | """Convert the `ROIs` structured arrays to :py:class:`dict` |
| | |
| | Parameters |
| | ---------- |
| | a |
| | Structured array containing ROI data |
| | |
| | Returns |
| | ------- |
| | One dict per ROI. Keys are "top_left", "bottom_right", and "bin", |
| | values are tuples whose first element is the x axis value and the |
| | second element is the y axis value. |
| | """ |
| |
|
| | dict_list = [] |
| | a = a[["startx", "starty", "endx", "endy", "groupx", "groupy"]] |
| | for sx, sy, ex, ey, gx, gy in a: |
| | roi_dict = { |
| | "top_left": [int(sx), int(sy)], |
| | "bottom_right": [int(ex), int(ey)], |
| | "bin": [int(gx), int(gy)], |
| | } |
| | dict_list.append(roi_dict) |
| | return dict_list |
| |
|