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|
| | #include <config.h> |
| |
|
| | #include <stdio.h> |
| | #include <unistd.h> |
| | #include <fcntl.h> |
| | #include <string.h> |
| |
|
| | #include "htslib/hts.h" |
| | #include "htslib/hfile.h" |
| | #include "samtools.h" |
| | #include "version.h" |
| |
|
| | int bam_taf2baf(int argc, char *argv[]); |
| | int bam_mpileup(int argc, char *argv[]); |
| | int bam_merge(int argc, char *argv[]); |
| | int bam_index(int argc, char *argv[]); |
| | int bam_sort(int argc, char *argv[]); |
| | int bam_tview_main(int argc, char *argv[]); |
| | int bam_mating(int argc, char *argv[]); |
| | int bam_rmdup(int argc, char *argv[]); |
| | int bam_flagstat(int argc, char *argv[]); |
| | int bam_fillmd(int argc, char *argv[]); |
| | int bam_idxstats(int argc, char *argv[]); |
| | int bam_markdup(int argc, char *argv[]); |
| | int main_samview(int argc, char *argv[]); |
| | int main_head(int argc, char *argv[]); |
| | int main_reheader(int argc, char *argv[]); |
| | int main_cut_target(int argc, char *argv[]); |
| | int main_phase(int argc, char *argv[]); |
| | int main_cat(int argc, char *argv[]); |
| | int main_depth(int argc, char *argv[]); |
| | int main_coverage(int argc, char *argv[]); |
| | int main_bam2fq(int argc, char *argv[]); |
| | int main_pad2unpad(int argc, char *argv[]); |
| | int main_bedcov(int argc, char *argv[]); |
| | int main_bamshuf(int argc, char *argv[]); |
| | int main_stats(int argc, char *argv[]); |
| | int main_flags(int argc, char *argv[]); |
| | int main_split(int argc, char *argv[]); |
| | int main_quickcheck(int argc, char *argv[]); |
| | int main_addreplacerg(int argc, char *argv[]); |
| | int faidx_main(int argc, char *argv[]); |
| | int dict_main(int argc, char *argv[]); |
| | int fqidx_main(int argc, char *argv[]); |
| | int amplicon_clip_main(int argc, char *argv[]); |
| | int main_ampliconstats(int argc, char *argv[]); |
| | int main_import(int argc, char *argv[]); |
| | int main_samples(int argc, char *argv[]); |
| | int main_consensus(int argc, char *argv[]); |
| | int main_reference(int argc, char *argv[]); |
| | int main_reset(int argc, char *argv[]); |
| | int main_cram_size(int argc, char *argv[]); |
| |
|
| | const char *samtools_version() |
| | { |
| | return SAMTOOLS_VERSION; |
| | } |
| |
|
| | |
| | const char *samtools_feature_string(void) { |
| | const char *fmt = |
| |
|
| | #ifdef PACKAGE_URL |
| | "build=configure " |
| | #else |
| | "build=Makefile " |
| | #endif |
| |
|
| | #ifdef HAVE_CURSES |
| | "curses=yes " |
| | #else |
| | "curses=no " |
| | #endif |
| | ; |
| |
|
| | return fmt; |
| | } |
| |
|
| | static void long_version(void) { |
| | printf("samtools %s\n" |
| | "Using htslib %s\n" |
| | "Copyright (C) 2023 Genome Research Ltd.\n", |
| | samtools_version(), hts_version()); |
| |
|
| | printf("\nSamtools compilation details:\n"); |
| | printf(" Features: %s\n", samtools_feature_string()); |
| | printf(" CC: %s\n", SAMTOOLS_CC); |
| | printf(" CPPFLAGS: %s\n", SAMTOOLS_CPPFLAGS); |
| | printf(" CFLAGS: %s\n", SAMTOOLS_CFLAGS); |
| | printf(" LDFLAGS: %s\n", SAMTOOLS_LDFLAGS); |
| | printf(" HTSDIR: %s\n", SAMTOOLS_HTSDIR); |
| | printf(" LIBS: %s\n", SAMTOOLS_LIBS); |
| | printf(" CURSES_LIB: %s\n", SAMTOOLS_CURSES_LIB); |
| |
|
| | printf("\nHTSlib compilation details:\n"); |
| | printf(" Features: %s\n", hts_feature_string()); |
| | printf(" CC: %s\n", hts_test_feature(HTS_FEATURE_CC)); |
| | printf(" CPPFLAGS: %s\n", hts_test_feature(HTS_FEATURE_CPPFLAGS)); |
| | printf(" CFLAGS: %s\n", hts_test_feature(HTS_FEATURE_CFLAGS)); |
| | printf(" LDFLAGS: %s\n", hts_test_feature(HTS_FEATURE_LDFLAGS)); |
| |
|
| | |
| | printf("\nHTSlib URL scheme handlers present:\n"); |
| | const char *plugins[100]; |
| | int np = 100, i, j; |
| |
|
| | if (hfile_list_plugins(plugins, &np) < 0) |
| | return; |
| |
|
| | for (i = 0; i < np; i++) { |
| | const char *sc_list[100]; |
| | int nschemes = 100; |
| | if (hfile_list_schemes(plugins[i], sc_list, &nschemes) < 0) |
| | return; |
| |
|
| | printf(" %s:\t", plugins[i]); |
| | for (j = 0; j < nschemes; j++) |
| | printf(" %s%c", sc_list[j], ",\n"[j+1==nschemes]); |
| | } |
| | } |
| |
|
| | static void usage(FILE *fp) |
| | { |
| | |
| |
|
| | fprintf(fp, |
| | "\n" |
| | "Program: samtools (Tools for alignments in the SAM format)\n" |
| | "Version: %s (using htslib %s)\n\n", samtools_version(), hts_version()); |
| | fprintf(fp, |
| | "Usage: samtools <command> [options]\n" |
| | "\n" |
| | "Commands:\n" |
| | " -- Indexing\n" |
| | " dict create a sequence dictionary file\n" |
| | " faidx index/extract FASTA\n" |
| | " fqidx index/extract FASTQ\n" |
| | " index index alignment\n" |
| | "\n" |
| | " -- Editing\n" |
| | " calmd recalculate MD/NM tags and '=' bases\n" |
| | " fixmate fix mate information\n" |
| | " reheader replace BAM header\n" |
| | " targetcut cut fosmid regions (for fosmid pool only)\n" |
| | " addreplacerg adds or replaces RG tags\n" |
| | " markdup mark duplicates\n" |
| | " ampliconclip clip oligos from the end of reads\n" |
| | "\n" |
| | " -- File operations\n" |
| | " collate shuffle and group alignments by name\n" |
| | " cat concatenate BAMs\n" |
| | " consensus produce a consensus Pileup/FASTA/FASTQ\n" |
| | " merge merge sorted alignments\n" |
| | " mpileup multi-way pileup\n" |
| | " sort sort alignment file\n" |
| | " split splits a file by read group\n" |
| | " quickcheck quickly check if SAM/BAM/CRAM file appears intact\n" |
| | " fastq converts a BAM to a FASTQ\n" |
| | " fasta converts a BAM to a FASTA\n" |
| | " import Converts FASTA or FASTQ files to SAM/BAM/CRAM\n" |
| | " reference Generates a reference from aligned data\n" |
| | " reset Reverts aligner changes in reads\n" |
| | "\n" |
| | " -- Statistics\n" |
| | " bedcov read depth per BED region\n" |
| | " coverage alignment depth and percent coverage\n" |
| | " depth compute the depth\n" |
| | " flagstat simple stats\n" |
| | " idxstats BAM index stats\n" |
| | " cram-size list CRAM Content-ID and Data-Series sizes\n" |
| | " phase phase heterozygotes\n" |
| | " stats generate stats (former bamcheck)\n" |
| | " ampliconstats generate amplicon specific stats\n" |
| | "\n" |
| | " -- Viewing\n" |
| | " flags explain BAM flags\n" |
| | " head header viewer\n" |
| | " tview text alignment viewer\n" |
| | " view SAM<->BAM<->CRAM conversion\n" |
| | " depad convert padded BAM to unpadded BAM\n" |
| | " samples list the samples in a set of SAM/BAM/CRAM files\n" |
| | "\n" |
| | " -- Misc\n" |
| | " help [cmd] display this help message or help for [cmd]\n" |
| | " version detailed version information\n" |
| | "\n"); |
| | } |
| |
|
| | |
| | |
| | |
| | |
| | |
| | |
| | |
| | |
| | #ifdef _WIN32 |
| | int _CRT_glob = 0; |
| | #endif |
| |
|
| | int main(int argc, char *argv[]) |
| | { |
| | #ifdef _WIN32 |
| | setmode(fileno(stdout), O_BINARY); |
| | setmode(fileno(stdin), O_BINARY); |
| | #endif |
| | if (argc < 2) { usage(stderr); return 1; } |
| |
|
| | if (strcmp(argv[1], "help") == 0 || strcmp(argv[1], "--help") == 0) { |
| | if (argc == 2) { usage(stdout); return 0; } |
| |
|
| | |
| | |
| | |
| | argv++; |
| | argc = 2; |
| | } |
| |
|
| | int ret = 0; |
| | if (strcmp(argv[1], "view") == 0) ret = main_samview(argc-1, argv+1); |
| | else if (strcmp(argv[1], "import") == 0) ret = main_import(argc-1, argv+1); |
| | else if (strcmp(argv[1], "mpileup") == 0) ret = bam_mpileup(argc-1, argv+1); |
| | else if (strcmp(argv[1], "merge") == 0) ret = bam_merge(argc-1, argv+1); |
| | else if (strcmp(argv[1], "sort") == 0) ret = bam_sort(argc-1, argv+1); |
| | else if (strcmp(argv[1], "index") == 0) ret = bam_index(argc-1, argv+1); |
| | else if (strcmp(argv[1], "idxstat") == 0 || |
| | strcmp(argv[1], "idxstats") == 0) ret = bam_idxstats(argc-1, argv+1); |
| | else if (strcmp(argv[1], "faidx") == 0) ret = faidx_main(argc-1, argv+1); |
| | else if (strcmp(argv[1], "fqidx") == 0) ret = fqidx_main(argc-1, argv+1); |
| | else if (strcmp(argv[1], "dict") == 0) ret = dict_main(argc-1, argv+1); |
| | else if (strcmp(argv[1], "head") == 0) ret = main_head(argc-1, argv+1); |
| | else if (strcmp(argv[1], "fixmate") == 0) ret = bam_mating(argc-1, argv+1); |
| | else if (strcmp(argv[1], "rmdup") == 0) ret = bam_rmdup(argc-1, argv+1); |
| | else if (strcmp(argv[1], "markdup") == 0) ret = bam_markdup(argc-1, argv+1); |
| | else if (strcmp(argv[1], "ampliconclip") == 0) ret = amplicon_clip_main(argc-1, argv+1); |
| | else if (strcmp(argv[1], "flagstat") == 0 || |
| | strcmp(argv[1], "flagstats") == 0) ret = bam_flagstat(argc-1, argv+1); |
| | else if (strcmp(argv[1], "calmd") == 0) ret = bam_fillmd(argc-1, argv+1); |
| | else if (strcmp(argv[1], "fillmd") == 0) ret = bam_fillmd(argc-1, argv+1); |
| | else if (strcmp(argv[1], "reheader") == 0) ret = main_reheader(argc-1, argv+1); |
| | else if (strcmp(argv[1], "cat") == 0) ret = main_cat(argc-1, argv+1); |
| | else if (strcmp(argv[1], "targetcut") == 0) ret = main_cut_target(argc-1, argv+1); |
| | else if (strcmp(argv[1], "phase") == 0) ret = main_phase(argc-1, argv+1); |
| | else if (strcmp(argv[1], "depth") == 0) ret = main_depth(argc-1, argv+1); |
| | else if (strcmp(argv[1], "coverage") == 0) ret = main_coverage(argc-1, argv+1); |
| | else if (strcmp(argv[1], "bam2fq") == 0 || |
| | strcmp(argv[1], "fastq") == 0 || |
| | strcmp(argv[1], "fasta") == 0) ret = main_bam2fq(argc-1, argv+1); |
| | else if (strcmp(argv[1], "pad2unpad") == 0) ret = main_pad2unpad(argc-1, argv+1); |
| | else if (strcmp(argv[1], "depad") == 0) ret = main_pad2unpad(argc-1, argv+1); |
| | else if (strcmp(argv[1], "bedcov") == 0) ret = main_bedcov(argc-1, argv+1); |
| | else if (strcmp(argv[1], "bamshuf") == 0) ret = main_bamshuf(argc-1, argv+1); |
| | else if (strcmp(argv[1], "collate") == 0) ret = main_bamshuf(argc-1, argv+1); |
| | else if (strcmp(argv[1], "stat") == 0 || |
| | strcmp(argv[1], "stats") == 0) ret = main_stats(argc-1, argv+1); |
| | else if (strcmp(argv[1], "flag") == 0 || |
| | strcmp(argv[1], "flags") == 0) ret = main_flags(argc-1, argv+1); |
| | else if (strcmp(argv[1], "split") == 0) ret = main_split(argc-1, argv+1); |
| | else if (strcmp(argv[1], "quickcheck") == 0) ret = main_quickcheck(argc-1, argv+1); |
| | else if (strcmp(argv[1], "addreplacerg") == 0) ret = main_addreplacerg(argc-1, argv+1); |
| | else if (strcmp(argv[1], "pileup") == 0) { |
| | fprintf(stderr, "[main] The `pileup' command has been removed. Please use `mpileup' instead.\n"); |
| | return 1; |
| | } |
| | else if (strcmp(argv[1], "tview") == 0) ret = bam_tview_main(argc-1, argv+1); |
| | else if (strcmp(argv[1], "ampliconstats") == 0) ret = main_ampliconstats(argc-1, argv+1); |
| | else if (strcmp(argv[1], "samples") == 0) ret = main_samples(argc-1, argv+1); |
| | else if (strcmp(argv[1], "consensus") == 0) ret = main_consensus(argc-1, argv+1); |
| | else if (strcmp(argv[1], "reference") == 0) ret = main_reference(argc-1, argv+1); |
| | else if (strcmp(argv[1], "cram-size") == 0) ret = main_cram_size(argc-1, argv+1); |
| | else if (strcmp(argv[1], "version") == 0 || \ |
| | strcmp(argv[1], "--version") == 0) |
| | long_version(); |
| | else if (strcmp(argv[1], "--version-only") == 0) { |
| | printf("%s+htslib-%s\n", samtools_version(), hts_version()); |
| | } |
| | else if (strcmp(argv[1], "reset") == 0) ret = main_reset(argc-1, argv+1); |
| | else { |
| | fprintf(stderr, "[main] unrecognized command '%s'\n", argv[1]); |
| | return 1; |
| | } |
| | return ret; |
| | } |
| |
|