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""" Read SPE files. |
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|
|
|
|
This plugin supports reading files saved in the Princeton Instruments |
|
|
SPE file format. |
|
|
|
|
|
Parameters |
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|
---------- |
|
|
check_filesize : bool |
|
|
The number of frames in the file is stored in the file header. However, |
|
|
this number may be wrong for certain software. If this is `True` |
|
|
(default), derive the number of frames also from the file size and |
|
|
raise a warning if the two values do not match. |
|
|
char_encoding : str |
|
|
Deprecated. Exists for backwards compatibility; use ``char_encoding`` of |
|
|
``metadata`` instead. |
|
|
sdt_meta : bool |
|
|
Deprecated. Exists for backwards compatibility; use ``sdt_control`` of |
|
|
``metadata`` instead. |
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|
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|
Methods |
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|
------- |
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|
.. note:: |
|
|
Check the respective function for a list of supported kwargs and detailed |
|
|
documentation. |
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|
|
|
.. autosummary:: |
|
|
:toctree: |
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|
|
|
SpePlugin.read |
|
|
SpePlugin.iter |
|
|
SpePlugin.properties |
|
|
SpePlugin.metadata |
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|
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|
""" |
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|
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from datetime import datetime |
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|
import logging |
|
|
import os |
|
|
from typing import ( |
|
|
Any, |
|
|
Callable, |
|
|
Dict, |
|
|
Iterator, |
|
|
List, |
|
|
Mapping, |
|
|
Optional, |
|
|
Sequence, |
|
|
Tuple, |
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|
Union, |
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|
) |
|
|
import warnings |
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|
|
|
|
import numpy as np |
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|
|
|
|
from ..core.request import Request, IOMode, InitializationError |
|
|
from ..core.v3_plugin_api import PluginV3, ImageProperties |
|
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|
|
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|
|
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logger = logging.getLogger(__name__) |
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|
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|
|
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class Spec: |
|
|
"""SPE file specification data |
|
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|
|
|
Tuples of (offset, datatype, count), where offset is the offset in the SPE |
|
|
file and datatype is the datatype as used in `numpy.fromfile`() |
|
|
|
|
|
`data_start` is the offset of actual image data. |
|
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|
|
|
`dtypes` translates SPE datatypes (0...4) to numpy ones, e. g. dtypes[0] |
|
|
is dtype("<f") (which is np.float32). |
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|
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|
`controllers` maps the `type` metadata to a human readable name |
|
|
|
|
|
`readout_modes` maps the `readoutMode` metadata to something human readable |
|
|
although this may not be accurate since there is next to no documentation |
|
|
to be found. |
|
|
""" |
|
|
|
|
|
basic = { |
|
|
"datatype": (108, "<h"), |
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|
"xdim": (42, "<H"), |
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|
"ydim": (656, "<H"), |
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|
"xml_footer_offset": (678, "<Q"), |
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|
"NumFrames": (1446, "<i"), |
|
|
"file_header_ver": (1992, "<f"), |
|
|
} |
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metadata = { |
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|
|
|
"NumROI": (1510, "<h"), |
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|
"ROIs": ( |
|
|
1512, |
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|
np.dtype( |
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[ |
|
|
("startx", "<H"), |
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|
("endx", "<H"), |
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|
("groupx", "<H"), |
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|
("starty", "<H"), |
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|
("endy", "<H"), |
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|
("groupy", "<H"), |
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|
] |
|
|
), |
|
|
10, |
|
|
), |
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|
|
|
"xDimDet": (6, "<H"), |
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|
"yDimDet": (18, "<H"), |
|
|
"VChipXdim": (14, "<h"), |
|
|
"VChipYdim": (16, "<h"), |
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|
|
|
|
"controller_version": (0, "<h"), |
|
|
"logic_output": (2, "<h"), |
|
|
"amp_high_cap_low_noise": (4, "<H"), |
|
|
"mode": (8, "<h"), |
|
|
"exposure_sec": (10, "<f"), |
|
|
"date": (20, "<10S"), |
|
|
"detector_temp": (36, "<f"), |
|
|
"detector_type": (40, "<h"), |
|
|
"st_diode": (44, "<h"), |
|
|
"delay_time": (46, "<f"), |
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|
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|
|
"shutter_control": (50, "<H"), |
|
|
"absorb_live": (52, "<h"), |
|
|
"absorb_mode": (54, "<H"), |
|
|
"can_do_virtual_chip": (56, "<h"), |
|
|
"threshold_min_live": (58, "<h"), |
|
|
"threshold_min_val": (60, "<f"), |
|
|
"threshold_max_live": (64, "<h"), |
|
|
"threshold_max_val": (66, "<f"), |
|
|
"time_local": (172, "<7S"), |
|
|
"time_utc": (179, "<7S"), |
|
|
"adc_offset": (188, "<H"), |
|
|
"adc_rate": (190, "<H"), |
|
|
"adc_type": (192, "<H"), |
|
|
"adc_resolution": (194, "<H"), |
|
|
"adc_bit_adjust": (196, "<H"), |
|
|
"gain": (198, "<H"), |
|
|
"comments": (200, "<80S", 5), |
|
|
"geometric": (600, "<H"), |
|
|
"sw_version": (688, "<16S"), |
|
|
"spare_4": (742, "<436S"), |
|
|
"XPrePixels": (98, "<h"), |
|
|
"XPostPixels": (100, "<h"), |
|
|
"YPrePixels": (102, "<h"), |
|
|
"YPostPixels": (104, "<h"), |
|
|
"readout_time": (672, "<f"), |
|
|
"xml_footer_offset": (678, "<Q"), |
|
|
"type": (704, "<h"), |
|
|
"clockspeed_us": (1428, "<f"), |
|
|
"readout_mode": (1480, "<H"), |
|
|
"window_size": (1482, "<H"), |
|
|
"file_header_ver": (1992, "<f"), |
|
|
} |
|
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|
|
|
data_start = 4100 |
|
|
|
|
|
dtypes = { |
|
|
0: np.dtype(np.float32), |
|
|
1: np.dtype(np.int32), |
|
|
2: np.dtype(np.int16), |
|
|
3: np.dtype(np.uint16), |
|
|
8: np.dtype(np.uint32), |
|
|
} |
|
|
|
|
|
controllers = [ |
|
|
"new120 (Type II)", |
|
|
"old120 (Type I)", |
|
|
"ST130", |
|
|
"ST121", |
|
|
"ST138", |
|
|
"DC131 (PentaMax)", |
|
|
"ST133 (MicroMax/Roper)", |
|
|
"ST135 (GPIB)", |
|
|
"VTCCD", |
|
|
"ST116 (GPIB)", |
|
|
"OMA3 (GPIB)", |
|
|
"OMA4", |
|
|
] |
|
|
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|
|
readout_modes = ["full frame", "frame transfer", "kinetics"] |
|
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|
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|
|
no_decode = ["spare_4"] |
|
|
|
|
|
|
|
|
class SDTControlSpec: |
|
|
"""Extract metadata written by the SDT-control software |
|
|
|
|
|
Some of it is encoded in the comment strings |
|
|
(see :py:meth:`parse_comments`). Also, date and time are encoded in a |
|
|
peculiar way (see :py:meth:`get_datetime`). Use :py:meth:`extract_metadata` |
|
|
to update the metadata dict. |
|
|
""" |
|
|
|
|
|
months = { |
|
|
|
|
|
"Jän": 1, |
|
|
"Jan": 1, |
|
|
"Feb": 2, |
|
|
"Mär": 3, |
|
|
"Mar": 3, |
|
|
"Apr": 4, |
|
|
"Mai": 5, |
|
|
"May": 5, |
|
|
"Jun": 6, |
|
|
"Jul": 7, |
|
|
"Aug": 8, |
|
|
"Sep": 9, |
|
|
"Okt": 10, |
|
|
"Oct": 10, |
|
|
"Nov": 11, |
|
|
"Dez": 12, |
|
|
"Dec": 12, |
|
|
} |
|
|
|
|
|
sequence_types = { |
|
|
|
|
|
"SEQU": "standard", |
|
|
"SETO": "TOCCSL", |
|
|
"KINE": "kinetics", |
|
|
"SEAR": "arbitrary", |
|
|
} |
|
|
|
|
|
class CommentDesc: |
|
|
"""Describe how to extract a metadata entry from a comment string""" |
|
|
|
|
|
n: int |
|
|
"""Which of the 5 SPE comment fields to use.""" |
|
|
slice: slice |
|
|
"""Which characters from the `n`-th comment to use.""" |
|
|
cvt: Callable[[str], Any] |
|
|
"""How to convert characters to something useful.""" |
|
|
scale: Union[None, float] |
|
|
"""Optional scaling factor for numbers""" |
|
|
|
|
|
def __init__( |
|
|
self, |
|
|
n: int, |
|
|
slice: slice, |
|
|
cvt: Callable[[str], Any] = str, |
|
|
scale: Optional[float] = None, |
|
|
): |
|
|
self.n = n |
|
|
self.slice = slice |
|
|
self.cvt = cvt |
|
|
self.scale = scale |
|
|
|
|
|
comment_fields = { |
|
|
(5, 0): { |
|
|
"sdt_major_version": CommentDesc(4, slice(66, 68), int), |
|
|
"sdt_minor_version": CommentDesc(4, slice(68, 70), int), |
|
|
"sdt_controller_name": CommentDesc(4, slice(0, 6), str), |
|
|
"exposure_time": CommentDesc(1, slice(64, 73), float, 10**-6), |
|
|
"color_code": CommentDesc(4, slice(10, 14), str), |
|
|
"detection_channels": CommentDesc(4, slice(15, 16), int), |
|
|
"background_subtraction": CommentDesc(4, 14, lambda x: x == "B"), |
|
|
"em_active": CommentDesc(4, 32, lambda x: x == "E"), |
|
|
"em_gain": CommentDesc(4, slice(28, 32), int), |
|
|
"modulation_active": CommentDesc(4, 33, lambda x: x == "A"), |
|
|
"pixel_size": CommentDesc(4, slice(25, 28), float, 0.1), |
|
|
"sequence_type": CommentDesc( |
|
|
4, slice(6, 10), lambda x: __class__.sequence_types[x] |
|
|
), |
|
|
"grid": CommentDesc(4, slice(16, 25), float, 10**-6), |
|
|
"n_macro": CommentDesc(1, slice(0, 4), int), |
|
|
"delay_macro": CommentDesc(1, slice(10, 19), float, 10**-3), |
|
|
"n_mini": CommentDesc(1, slice(4, 7), int), |
|
|
"delay_mini": CommentDesc(1, slice(19, 28), float, 10**-6), |
|
|
"n_micro": CommentDesc(1, slice(7, 10), int), |
|
|
"delay_micro": CommentDesc(1, slice(28, 37), float, 10**-6), |
|
|
"n_subpics": CommentDesc(1, slice(7, 10), int), |
|
|
"delay_shutter": CommentDesc(1, slice(73, 79), float, 10**-6), |
|
|
"delay_prebleach": CommentDesc(1, slice(37, 46), float, 10**-6), |
|
|
"bleach_time": CommentDesc(1, slice(46, 55), float, 10**-6), |
|
|
"recovery_time": CommentDesc(1, slice(55, 64), float, 10**-6), |
|
|
}, |
|
|
(5, 1): { |
|
|
"bleach_piezo_active": CommentDesc(4, slice(34, 35), lambda x: x == "z") |
|
|
}, |
|
|
} |
|
|
|
|
|
@staticmethod |
|
|
def get_comment_version(comments: Sequence[str]) -> Tuple[int, int]: |
|
|
"""Get the version of SDT-control metadata encoded in the comments |
|
|
|
|
|
Parameters |
|
|
---------- |
|
|
comments |
|
|
List of SPE file comments, typically ``metadata["comments"]``. |
|
|
|
|
|
Returns |
|
|
------- |
|
|
Major and minor version. ``-1, -1`` if detection failed. |
|
|
""" |
|
|
if comments[4][70:76] != "COMVER": |
|
|
return -1, -1 |
|
|
try: |
|
|
return int(comments[4][76:78]), int(comments[4][78:80]) |
|
|
except ValueError: |
|
|
return -1, -1 |
|
|
|
|
|
@staticmethod |
|
|
def parse_comments( |
|
|
comments: Sequence[str], version: Tuple[int, int] |
|
|
) -> Dict[str, Any]: |
|
|
"""Extract SDT-control metadata from comments |
|
|
|
|
|
Parameters |
|
|
---------- |
|
|
comments |
|
|
List of SPE file comments, typically ``metadata["comments"]``. |
|
|
version |
|
|
Major and minor version of SDT-control metadata format |
|
|
|
|
|
Returns |
|
|
------- |
|
|
Dict of metadata |
|
|
""" |
|
|
sdt_md = {} |
|
|
for minor in range(version[1] + 1): |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
try: |
|
|
cmt = __class__.comment_fields[version[0], minor] |
|
|
except KeyError: |
|
|
continue |
|
|
for name, spec in cmt.items(): |
|
|
try: |
|
|
v = spec.cvt(comments[spec.n][spec.slice]) |
|
|
if spec.scale is not None: |
|
|
v *= spec.scale |
|
|
sdt_md[name] = v |
|
|
except Exception as e: |
|
|
warnings.warn( |
|
|
f"Failed to decode SDT-control metadata field `{name}`: {e}" |
|
|
) |
|
|
sdt_md[name] = None |
|
|
if version not in __class__.comment_fields: |
|
|
supported_ver = ", ".join( |
|
|
map(lambda x: f"{x[0]}.{x[1]:02}", __class__.comment_fields) |
|
|
) |
|
|
warnings.warn( |
|
|
f"Unsupported SDT-control metadata version {version[0]}.{version[1]:02}. " |
|
|
f"Only versions {supported_ver} are supported. " |
|
|
"Some or all SDT-control metadata may be missing." |
|
|
) |
|
|
comment = comments[0] + comments[2] |
|
|
sdt_md["comment"] = comment.strip() |
|
|
return sdt_md |
|
|
|
|
|
@staticmethod |
|
|
def get_datetime(date: str, time: str) -> Union[datetime, None]: |
|
|
"""Turn date and time saved by SDT-control into proper datetime object |
|
|
|
|
|
Parameters |
|
|
---------- |
|
|
date |
|
|
SPE file date, typically ``metadata["date"]``. |
|
|
time |
|
|
SPE file date, typically ``metadata["time_local"]``. |
|
|
|
|
|
Returns |
|
|
------- |
|
|
File's datetime if parsing was succsessful, else None. |
|
|
""" |
|
|
try: |
|
|
month = __class__.months[date[2:5]] |
|
|
return datetime( |
|
|
int(date[5:9]), |
|
|
month, |
|
|
int(date[0:2]), |
|
|
int(time[0:2]), |
|
|
int(time[2:4]), |
|
|
int(time[4:6]), |
|
|
) |
|
|
except Exception as e: |
|
|
logger.info(f"Failed to decode date from SDT-control metadata: {e}.") |
|
|
|
|
|
@staticmethod |
|
|
def extract_metadata(meta: Mapping, char_encoding: str = "latin1"): |
|
|
"""Extract SDT-control metadata from SPE metadata |
|
|
|
|
|
SDT-control stores some metadata in comments and other fields. |
|
|
Extract them and remove unused entries. |
|
|
|
|
|
Parameters |
|
|
---------- |
|
|
meta |
|
|
SPE file metadata. Modified in place. |
|
|
char_encoding |
|
|
Character encoding used to decode strings in the metadata. |
|
|
""" |
|
|
comver = __class__.get_comment_version(meta["comments"]) |
|
|
if any(c < 0 for c in comver): |
|
|
|
|
|
logger.debug("SDT-control comments not found.") |
|
|
return |
|
|
|
|
|
sdt_meta = __class__.parse_comments(meta["comments"], comver) |
|
|
meta.pop("comments") |
|
|
meta.update(sdt_meta) |
|
|
|
|
|
|
|
|
dt = __class__.get_datetime(meta["date"], meta["time_local"]) |
|
|
if dt: |
|
|
meta["datetime"] = dt |
|
|
meta.pop("date") |
|
|
meta.pop("time_local") |
|
|
|
|
|
sp4 = meta["spare_4"] |
|
|
try: |
|
|
meta["modulation_script"] = sp4.decode(char_encoding) |
|
|
meta.pop("spare_4") |
|
|
except UnicodeDecodeError: |
|
|
warnings.warn( |
|
|
"Failed to decode SDT-control laser " |
|
|
"modulation script. Bad char_encoding?" |
|
|
) |
|
|
|
|
|
|
|
|
meta.pop("time_utc") |
|
|
meta.pop("exposure_sec") |
|
|
|
|
|
|
|
|
class SpePlugin(PluginV3): |
|
|
def __init__( |
|
|
self, |
|
|
request: Request, |
|
|
check_filesize: bool = True, |
|
|
char_encoding: Optional[str] = None, |
|
|
sdt_meta: Optional[bool] = None, |
|
|
) -> None: |
|
|
"""Instantiate a new SPE file plugin object |
|
|
|
|
|
Parameters |
|
|
---------- |
|
|
request : Request |
|
|
A request object representing the resource to be operated on. |
|
|
check_filesize : bool |
|
|
If True, compute the number of frames from the filesize, compare it |
|
|
to the frame count in the file header, and raise a warning if the |
|
|
counts don't match. (Certain software may create files with |
|
|
char_encoding : str |
|
|
Deprecated. Exists for backwards compatibility; use ``char_encoding`` of |
|
|
``metadata`` instead. |
|
|
sdt_meta : bool |
|
|
Deprecated. Exists for backwards compatibility; use ``sdt_control`` of |
|
|
``metadata`` instead. |
|
|
|
|
|
""" |
|
|
|
|
|
super().__init__(request) |
|
|
if request.mode.io_mode == IOMode.write: |
|
|
raise InitializationError("cannot write SPE files") |
|
|
|
|
|
if char_encoding is not None: |
|
|
warnings.warn( |
|
|
"Passing `char_encoding` to the constructor is deprecated. " |
|
|
"Use `char_encoding` parameter of the `metadata()` method " |
|
|
"instead.", |
|
|
DeprecationWarning, |
|
|
) |
|
|
self._char_encoding = char_encoding |
|
|
if sdt_meta is not None: |
|
|
warnings.warn( |
|
|
"Passing `sdt_meta` to the constructor is deprecated. " |
|
|
"Use `sdt_control` parameter of the `metadata()` method " |
|
|
"instead.", |
|
|
DeprecationWarning, |
|
|
) |
|
|
self._sdt_meta = sdt_meta |
|
|
|
|
|
self._file = self.request.get_file() |
|
|
|
|
|
try: |
|
|
|
|
|
|
|
|
info = self._parse_header(Spec.basic, "latin1") |
|
|
self._file_header_ver = info["file_header_ver"] |
|
|
self._dtype = Spec.dtypes[info["datatype"]] |
|
|
self._shape = (info["ydim"], info["xdim"]) |
|
|
self._len = info["NumFrames"] |
|
|
|
|
|
if check_filesize: |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
if info["file_header_ver"] >= 3: |
|
|
data_end = info["xml_footer_offset"] |
|
|
else: |
|
|
self._file.seek(0, os.SEEK_END) |
|
|
data_end = self._file.tell() |
|
|
line = data_end - Spec.data_start |
|
|
line //= self._shape[0] * self._shape[1] * self._dtype.itemsize |
|
|
if line != self._len: |
|
|
warnings.warn( |
|
|
f"The file header of {self.request.filename} claims there are " |
|
|
f"{self._len} frames, but there are actually {line} frames." |
|
|
) |
|
|
self._len = min(line, self._len) |
|
|
self._file.seek(Spec.data_start) |
|
|
except Exception: |
|
|
raise InitializationError("SPE plugin cannot read the provided file.") |
|
|
|
|
|
def read(self, *, index: int = ...) -> np.ndarray: |
|
|
"""Read a frame or all frames from the file |
|
|
|
|
|
Parameters |
|
|
---------- |
|
|
index : int |
|
|
Select the index-th frame from the file. If index is `...`, |
|
|
select all frames and stack them along a new axis. |
|
|
|
|
|
Returns |
|
|
------- |
|
|
A Numpy array of pixel values. |
|
|
|
|
|
""" |
|
|
|
|
|
if index is Ellipsis: |
|
|
read_offset = Spec.data_start |
|
|
count = self._shape[0] * self._shape[1] * self._len |
|
|
out_shape = (self._len, *self._shape) |
|
|
elif index < 0: |
|
|
raise IndexError(f"Index `{index}` is smaller than 0.") |
|
|
elif index >= self._len: |
|
|
raise IndexError( |
|
|
f"Index `{index}` exceeds the number of frames stored in this file (`{self._len}`)." |
|
|
) |
|
|
else: |
|
|
read_offset = ( |
|
|
Spec.data_start |
|
|
+ index * self._shape[0] * self._shape[1] * self._dtype.itemsize |
|
|
) |
|
|
count = self._shape[0] * self._shape[1] |
|
|
out_shape = self._shape |
|
|
|
|
|
self._file.seek(read_offset) |
|
|
data = np.fromfile(self._file, dtype=self._dtype, count=count) |
|
|
return data.reshape(out_shape) |
|
|
|
|
|
def iter(self) -> Iterator[np.ndarray]: |
|
|
"""Iterate over the frames in the file |
|
|
|
|
|
Yields |
|
|
------ |
|
|
A Numpy array of pixel values. |
|
|
""" |
|
|
|
|
|
return (self.read(index=i) for i in range(self._len)) |
|
|
|
|
|
def metadata( |
|
|
self, |
|
|
index: int = ..., |
|
|
exclude_applied: bool = True, |
|
|
char_encoding: str = "latin1", |
|
|
sdt_control: bool = True, |
|
|
) -> Dict[str, Any]: |
|
|
"""SPE specific metadata. |
|
|
|
|
|
Parameters |
|
|
---------- |
|
|
index : int |
|
|
Ignored as SPE files only store global metadata. |
|
|
exclude_applied : bool |
|
|
Ignored. Exists for API compatibility. |
|
|
char_encoding : str |
|
|
The encoding to use when parsing strings. |
|
|
sdt_control : bool |
|
|
If `True`, decode special metadata written by the |
|
|
SDT-control software if present. |
|
|
|
|
|
Returns |
|
|
------- |
|
|
metadata : dict |
|
|
Key-value pairs of metadata. |
|
|
|
|
|
Notes |
|
|
----- |
|
|
SPE v3 stores metadata as XML, whereas SPE v2 uses a binary format. |
|
|
|
|
|
.. rubric:: Supported SPE v2 Metadata fields |
|
|
|
|
|
ROIs : list of dict |
|
|
Regions of interest used for recording images. Each dict has the |
|
|
"top_left" key containing x and y coordinates of the top left corner, |
|
|
the "bottom_right" key with x and y coordinates of the bottom right |
|
|
corner, and the "bin" key with number of binned pixels in x and y |
|
|
directions. |
|
|
comments : list of str |
|
|
The SPE format allows for 5 comment strings of 80 characters each. |
|
|
controller_version : int |
|
|
Hardware version |
|
|
logic_output : int |
|
|
Definition of output BNC |
|
|
amp_hi_cap_low_noise : int |
|
|
Amp switching mode |
|
|
mode : int |
|
|
Timing mode |
|
|
exp_sec : float |
|
|
Alternative exposure in seconds |
|
|
date : str |
|
|
Date string |
|
|
detector_temp : float |
|
|
Detector temperature |
|
|
detector_type : int |
|
|
CCD / diode array type |
|
|
st_diode : int |
|
|
Trigger diode |
|
|
delay_time : float |
|
|
Used with async mode |
|
|
shutter_control : int |
|
|
Normal, disabled open, or disabled closed |
|
|
absorb_live : bool |
|
|
on / off |
|
|
absorb_mode : int |
|
|
Reference strip or file |
|
|
can_do_virtual_chip : bool |
|
|
True or False whether chip can do virtual chip |
|
|
threshold_min_live : bool |
|
|
on / off |
|
|
threshold_min_val : float |
|
|
Threshold minimum value |
|
|
threshold_max_live : bool |
|
|
on / off |
|
|
threshold_max_val : float |
|
|
Threshold maximum value |
|
|
time_local : str |
|
|
Experiment local time |
|
|
time_utc : str |
|
|
Experiment UTC time |
|
|
adc_offset : int |
|
|
ADC offset |
|
|
adc_rate : int |
|
|
ADC rate |
|
|
adc_type : int |
|
|
ADC type |
|
|
adc_resolution : int |
|
|
ADC resolution |
|
|
adc_bit_adjust : int |
|
|
ADC bit adjust |
|
|
gain : int |
|
|
gain |
|
|
sw_version : str |
|
|
Version of software which created this file |
|
|
spare_4 : bytes |
|
|
Reserved space |
|
|
readout_time : float |
|
|
Experiment readout time |
|
|
type : str |
|
|
Controller type |
|
|
clockspeed_us : float |
|
|
Vertical clock speed in microseconds |
|
|
readout_mode : ["full frame", "frame transfer", "kinetics", ""] |
|
|
Readout mode. Empty string means that this was not set by the |
|
|
Software. |
|
|
window_size : int |
|
|
Window size for Kinetics mode |
|
|
file_header_ver : float |
|
|
File header version |
|
|
chip_size : [int, int] |
|
|
x and y dimensions of the camera chip |
|
|
virt_chip_size : [int, int] |
|
|
Virtual chip x and y dimensions |
|
|
pre_pixels : [int, int] |
|
|
Pre pixels in x and y dimensions |
|
|
post_pixels : [int, int], |
|
|
Post pixels in x and y dimensions |
|
|
geometric : list of {"rotate", "reverse", "flip"} |
|
|
Geometric operations |
|
|
sdt_major_version : int |
|
|
(only for files created by SDT-control) |
|
|
Major version of SDT-control software |
|
|
sdt_minor_version : int |
|
|
(only for files created by SDT-control) |
|
|
Minor version of SDT-control software |
|
|
sdt_controller_name : str |
|
|
(only for files created by SDT-control) |
|
|
Controller name |
|
|
exposure_time : float |
|
|
(only for files created by SDT-control) |
|
|
Exposure time in seconds |
|
|
color_code : str |
|
|
(only for files created by SDT-control) |
|
|
Color channels used |
|
|
detection_channels : int |
|
|
(only for files created by SDT-control) |
|
|
Number of channels |
|
|
background_subtraction : bool |
|
|
(only for files created by SDT-control) |
|
|
Whether background subtraction war turned on |
|
|
em_active : bool |
|
|
(only for files created by SDT-control) |
|
|
Whether EM was turned on |
|
|
em_gain : int |
|
|
(only for files created by SDT-control) |
|
|
EM gain |
|
|
modulation_active : bool |
|
|
(only for files created by SDT-control) |
|
|
Whether laser modulation (“attenuate”) was turned on |
|
|
pixel_size : float |
|
|
(only for files created by SDT-control) |
|
|
Camera pixel size |
|
|
sequence_type : str |
|
|
(only for files created by SDT-control) |
|
|
Type of sequnce (standard, TOCCSL, arbitrary, …) |
|
|
grid : float |
|
|
(only for files created by SDT-control) |
|
|
Sequence time unit (“grid size”) in seconds |
|
|
n_macro : int |
|
|
(only for files created by SDT-control) |
|
|
Number of macro loops |
|
|
delay_macro : float |
|
|
(only for files created by SDT-control) |
|
|
Time between macro loops in seconds |
|
|
n_mini : int |
|
|
(only for files created by SDT-control) |
|
|
Number of mini loops |
|
|
delay_mini : float |
|
|
(only for files created by SDT-control) |
|
|
Time between mini loops in seconds |
|
|
n_micro : int (only for files created by SDT-control) |
|
|
Number of micro loops |
|
|
delay_micro : float (only for files created by SDT-control) |
|
|
Time between micro loops in seconds |
|
|
n_subpics : int |
|
|
(only for files created by SDT-control) |
|
|
Number of sub-pictures |
|
|
delay_shutter : float |
|
|
(only for files created by SDT-control) |
|
|
Camera shutter delay in seconds |
|
|
delay_prebleach : float |
|
|
(only for files created by SDT-control) |
|
|
Pre-bleach delay in seconds |
|
|
bleach_time : float |
|
|
(only for files created by SDT-control) |
|
|
Bleaching time in seconds |
|
|
recovery_time : float |
|
|
(only for files created by SDT-control) |
|
|
Recovery time in seconds |
|
|
comment : str |
|
|
(only for files created by SDT-control) |
|
|
User-entered comment. This replaces the "comments" field. |
|
|
datetime : datetime.datetime |
|
|
(only for files created by SDT-control) |
|
|
Combines the "date" and "time_local" keys. The latter two plus |
|
|
"time_utc" are removed. |
|
|
modulation_script : str |
|
|
(only for files created by SDT-control) |
|
|
Laser modulation script. Replaces the "spare_4" key. |
|
|
bleach_piezo_active : bool |
|
|
(only for files created by SDT-control) |
|
|
Whether piezo for bleaching was enabled |
|
|
""" |
|
|
|
|
|
if self._file_header_ver < 3: |
|
|
if self._char_encoding is not None: |
|
|
char_encoding = self._char_encoding |
|
|
if self._sdt_meta is not None: |
|
|
sdt_control = self._sdt_meta |
|
|
return self._metadata_pre_v3(char_encoding, sdt_control) |
|
|
return self._metadata_post_v3() |
|
|
|
|
|
def _metadata_pre_v3(self, char_encoding: str, sdt_control: bool) -> Dict[str, Any]: |
|
|
"""Extract metadata from SPE v2 files |
|
|
|
|
|
Parameters |
|
|
---------- |
|
|
char_encoding |
|
|
String character encoding |
|
|
sdt_control |
|
|
If `True`, try to decode special metadata written by the |
|
|
SDT-control software. |
|
|
|
|
|
Returns |
|
|
------- |
|
|
dict mapping metadata names to values. |
|
|
|
|
|
""" |
|
|
|
|
|
m = self._parse_header(Spec.metadata, char_encoding) |
|
|
|
|
|
nr = m.pop("NumROI", None) |
|
|
nr = 1 if nr < 1 else nr |
|
|
m["ROIs"] = roi_array_to_dict(m["ROIs"][:nr]) |
|
|
|
|
|
|
|
|
m["chip_size"] = [m.pop(k, None) for k in ("xDimDet", "yDimDet")] |
|
|
m["virt_chip_size"] = [m.pop(k, None) for k in ("VChipXdim", "VChipYdim")] |
|
|
m["pre_pixels"] = [m.pop(k, None) for k in ("XPrePixels", "YPrePixels")] |
|
|
m["post_pixels"] = [m.pop(k, None) for k in ("XPostPixels", "YPostPixels")] |
|
|
|
|
|
|
|
|
m["comments"] = [str(c) for c in m["comments"]] |
|
|
|
|
|
|
|
|
g = [] |
|
|
f = m.pop("geometric", 0) |
|
|
if f & 1: |
|
|
g.append("rotate") |
|
|
if f & 2: |
|
|
g.append("reverse") |
|
|
if f & 4: |
|
|
g.append("flip") |
|
|
m["geometric"] = g |
|
|
|
|
|
|
|
|
t = m["type"] |
|
|
if 1 <= t <= len(Spec.controllers): |
|
|
m["type"] = Spec.controllers[t - 1] |
|
|
else: |
|
|
m["type"] = None |
|
|
r = m["readout_mode"] |
|
|
if 1 <= r <= len(Spec.readout_modes): |
|
|
m["readout_mode"] = Spec.readout_modes[r - 1] |
|
|
else: |
|
|
m["readout_mode"] = None |
|
|
|
|
|
|
|
|
for k in ( |
|
|
"absorb_live", |
|
|
"can_do_virtual_chip", |
|
|
"threshold_min_live", |
|
|
"threshold_max_live", |
|
|
): |
|
|
m[k] = bool(m[k]) |
|
|
|
|
|
|
|
|
if sdt_control: |
|
|
SDTControlSpec.extract_metadata(m, char_encoding) |
|
|
|
|
|
return m |
|
|
|
|
|
def _metadata_post_v3(self) -> Dict[str, Any]: |
|
|
"""Extract XML metadata from SPE v3 files |
|
|
|
|
|
Returns |
|
|
------- |
|
|
dict with key `"__xml"`, whose value is the XML metadata |
|
|
""" |
|
|
|
|
|
info = self._parse_header(Spec.basic, "latin1") |
|
|
self._file.seek(info["xml_footer_offset"]) |
|
|
xml = self._file.read() |
|
|
return {"__xml": xml} |
|
|
|
|
|
def properties(self, index: int = ...) -> ImageProperties: |
|
|
"""Standardized ndimage metadata. |
|
|
|
|
|
Parameters |
|
|
---------- |
|
|
index : int |
|
|
If the index is an integer, select the index-th frame and return |
|
|
its properties. If index is an Ellipsis (...), return the |
|
|
properties of all frames in the file stacked along a new batch |
|
|
dimension. |
|
|
|
|
|
Returns |
|
|
------- |
|
|
properties : ImageProperties |
|
|
A dataclass filled with standardized image metadata. |
|
|
""" |
|
|
|
|
|
if index is Ellipsis: |
|
|
return ImageProperties( |
|
|
shape=(self._len, *self._shape), |
|
|
dtype=self._dtype, |
|
|
n_images=self._len, |
|
|
is_batch=True, |
|
|
) |
|
|
return ImageProperties(shape=self._shape, dtype=self._dtype, is_batch=False) |
|
|
|
|
|
def _parse_header( |
|
|
self, spec: Mapping[str, Tuple], char_encoding: str |
|
|
) -> Dict[str, Any]: |
|
|
"""Get information from SPE file header |
|
|
|
|
|
Parameters |
|
|
---------- |
|
|
spec |
|
|
Maps header entry name to its location, data type description and |
|
|
optionally number of entries. See :py:attr:`Spec.basic` and |
|
|
:py:attr:`Spec.metadata`. |
|
|
char_encoding |
|
|
String character encoding |
|
|
|
|
|
Returns |
|
|
------- |
|
|
Dict mapping header entry name to its value |
|
|
""" |
|
|
|
|
|
ret = {} |
|
|
|
|
|
decode = np.vectorize(lambda x: x.decode(char_encoding)) |
|
|
|
|
|
for name, sp in spec.items(): |
|
|
self._file.seek(sp[0]) |
|
|
cnt = 1 if len(sp) < 3 else sp[2] |
|
|
v = np.fromfile(self._file, dtype=sp[1], count=cnt) |
|
|
if v.dtype.kind == "S" and name not in Spec.no_decode: |
|
|
|
|
|
try: |
|
|
v = decode(v) |
|
|
except Exception: |
|
|
warnings.warn( |
|
|
f'Failed to decode "{name}" metadata ' |
|
|
"string. Check `char_encoding` parameter." |
|
|
) |
|
|
|
|
|
try: |
|
|
|
|
|
|
|
|
v = v.item() |
|
|
except ValueError: |
|
|
v = np.squeeze(v) |
|
|
ret[name] = v |
|
|
return ret |
|
|
|
|
|
|
|
|
def roi_array_to_dict(a: np.ndarray) -> List[Dict[str, List[int]]]: |
|
|
"""Convert the `ROIs` structured arrays to :py:class:`dict` |
|
|
|
|
|
Parameters |
|
|
---------- |
|
|
a |
|
|
Structured array containing ROI data |
|
|
|
|
|
Returns |
|
|
------- |
|
|
One dict per ROI. Keys are "top_left", "bottom_right", and "bin", |
|
|
values are tuples whose first element is the x axis value and the |
|
|
second element is the y axis value. |
|
|
""" |
|
|
|
|
|
dict_list = [] |
|
|
a = a[["startx", "starty", "endx", "endy", "groupx", "groupy"]] |
|
|
for sx, sy, ex, ey, gx, gy in a: |
|
|
roi_dict = { |
|
|
"top_left": [int(sx), int(sy)], |
|
|
"bottom_right": [int(ex), int(ey)], |
|
|
"bin": [int(gx), int(gy)], |
|
|
} |
|
|
dict_list.append(roi_dict) |
|
|
return dict_list |
|
|
|