Z-Screen Data Shape Explorer

Interactive structural visualization of nanowells, devices, cell lines, and libraries

Total Observations (Nanowells)
768,162
Mapped across 49 devices
Total Features (Genes)
46,944
In counts.h5ad matrix
Screening Devices
49
Numbered 0 to 48
Unique Compounds
142,227
Including 142.1k building blocks

๐Ÿ”ฌ Nanowell Architecture Specification

Each observation (row) in the dataset corresponds to a single **nanowell** rather than a single cell. Each nanowell measures **90 microns in diameter by 120 microns tall** and holds approximately **2 to 10 cells**. The transcriptomic profiles represent the pooled expression of the cells inside each nanowell.

Nanowells per Cell Line Total profiled nanowells
Dataset Role Split By screening category (wells)
Key Structural Insights

๐Ÿ”ฌ Multiplexed Chip Architecture

Each physical device runs exactly one cell line. This eliminates cell mixing within a single microfluidic chip, enabling clean downstream sorting. However, multiple chemical libraries are pooled on each chip (ranging from 2 to 8 libraries per device).

๐Ÿงช Named Control Compounds vs. Combinatorial Libraries

The screen features a split between **One-Bead One-Compound (OBOC) combinatorial libraries** (testing 142.1k chemical precursor fragments across 353.3k wells) and **Named Control Compounds** (testing 78 fully synthesized target compounds across 311.3k wells).

๐Ÿ›ก๏ธ No-Compound Control Beads

Out of 49 devices, 24 devices contain no-compound controls (including capture-only control bead library `ZEL026-2` and water/vehicle controls like `ZIC005`). Only Device 11 lacks control beads entirely, profiling named control compounds exclusively.

Screening Matrix Map
Nanowell counts mapped per library (rows) and cell line (columns)
Not Screened
low (< 2,000 wells)
medium (2k - 25k wells)
high (> 25k wells)
Device-Level Screen Map
Search, filter, and inspect cell line labels and pooled libraries per device
Device ID Cell Line Libraries Screened Nanowell Count Treatment Split
Screened Chemical Libraries & Control Sets
Details of compound counts, SMILES configurations, and nomenclature types