# Copyright (c) Meta Platforms, Inc. and affiliates. # All rights reserved. # # This source code is licensed under the BSD-style license found in the # LICENSE file in the root directory of this source tree. """ Data models for Pathway Analysis Environment. Supports pipeline-style episodes (count matrix + sample metadata + gene sets) with PyDESeq2 differential expression, Fisher ORA, overlap-aware summaries, and HTML step traces. """ from __future__ import annotations from typing import Any, Dict, List, Optional from openenv.core.env_server import Action, Observation, State from pydantic import Field class PathwayAction(Action): """ Action for the Pathway Analysis environment. action_type: - ``inspect_dataset``: describe available samples and conditions. - ``understand_experiment_design``: **(1)** Summarize groups (conditions, sample counts); optionally **(2)** validate ``condition_a``/``condition_b`` as reference/alternate for DGE (does not run DESeq2). Valid pairs feed ``run_differential_expression`` when DE omits conditions. **(3)** Pathway steps follow DE. - ``run_differential_expression``: PyDESeq2 contrast (needs ``condition_a`` / ``condition_b`` when using count-matrix cases). - ``run_pathway_enrichment``: ORA on DE genes vs pathway gene sets. - ``compare_pathways``: contrast exclusive vs shared DE support between two pathways (``pathway_a``, ``pathway_b``). - ``submit_answer``: submit ``hypothesis`` pathway name and end episode. """ action_type: str condition_a: Optional[str] = None condition_b: Optional[str] = None gene_list: Optional[List[str]] = None hypothesis: Optional[str] = None pathway_a: Optional[str] = None pathway_b: Optional[str] = None class PathwayObservation(Observation): """Observation with optional rich DE / ORA structures (JSON-serializable).""" message: str = "" available_conditions: List[str] = Field(default_factory=list) top_genes: List[str] = Field(default_factory=list) top_pathways: List[str] = Field(default_factory=list) de_genes: List[Dict[str, Any]] = Field(default_factory=list) pathway_enrichment: List[Dict[str, Any]] = Field(default_factory=list) pathway_comparison: Optional[Dict[str, Any]] = None overlap_summary: Optional[Dict[str, Any]] = None statistical_ambiguity: Optional[Dict[str, Any]] = None trace_path: Optional[str] = None experiment_design: Optional[Dict[str, Any]] = None class PathwayState(State): """Agent-visible episode state (ground truth is never included).""" conditions: List[str] = Field(default_factory=list) de_run: bool = False enrichment_run: bool = False is_done: bool = False pipeline_mode: bool = False strict_mode: bool = False legacy_mode: bool = False eval_mode: bool = True max_steps: int = 30 design_understood: bool = False validated_reference: Optional[str] = None validated_alternate: Optional[str] = None