When the time came to begin You Only Live Twice , the producers were faced with the problem of a disenchanted star . Sean Connery had stated that he was tired of playing James Bond and all of the associated commitment ( time spent filming and publicising each movie ) , together with finding it difficult to do other work , which would potentially lead to typecasting . Saltzman and Broccoli were able to persuade Connery by increasing his fee for the film , but geared up to look for a replacement .


Sean Connery 's then wife Diane Cilento did the swimming scenes for at least five Japanese actresses , including Mie Hama . Martial arts expert Donn F. Draeger provided martial arts training , and also doubled for Connery . Lewis Gilbert 's regular editor , Thelma Connell , was originally hired to edit the film . However , after her initial , almost three @-@ hour cut received a terrible response from test audiences , Peter R. Hunt was asked to re @-@ edit the film . Hunt 's cut proved a much greater success , and he was awarded the director 's chair on the next film as a result .


You Only Live Twice ( 1967 ) is the fifth spy film in the James Bond series , and the fifth to star Sean Connery as the fictional MI6 agent James Bond . The film 's screenplay was written by Roald Dahl , and loosely based on Ian Fleming 's 1964 novel of the same name . It is the first James Bond film to discard most of Fleming 's plot , using only a few characters and locations from the book as the background for an entirely new story .


About 35 officer ’ s cabins were vacated and additional bunks were installed , with the Empress taking over the Captain 's cabin . On the morning of 12 April the ship anchored off Halki Island , about 12 miles ( 19 km ) from Constantinople , due to some uncertainty over the final destination for the Russian Royal family . On 16 April Grand Duke Nicholas Nikolaevich and his wife the Grand Duchess Anastasia , the Grand Duke Peter Nicholaievitch and his wife Grand Duchess Milica , Princess Marina , Prince Roman , Count and Countess Tyszkiewich , Baron and Baroness Staal , Mr Boldyreff and Dr Malama with their respective servants left the ship and boarded HMS Lord Nelson destined for Genoa . They were replaced by Count Dimitri and Countess Sophia Mengden , Count George and Countess Irina Mengden , Countess Vera Mengden , Count Nicholas Mengden , Madame Helena Erchoff and two maids . On the morning of 18 April , Good Friday , the ship sailed for Malta . The ship departed on 18 April , bound for Malta to deposit the Russians , before returning to Constantinople .


The miners found that the row of cottages that served as their homes had been devastated and some of their families killed , seemingly at random . One found his family alive and safe in a makeshift hospital , but another emerged to discover his wife and four children had died . Fifteen @-@ year @-@ old Lillian Clark , working a late shift that night in the town 's boarding house , had been given permission to stay overnight for the first time . She was the only member of her family to survive . Her father was working outside the mine when the slide hit , while her mother and six siblings were buried in their home . All 12 men living at the CPR work camp were killed , but 128 more who were scheduled to move into the camp the day before the slide had not arrived — the train that was supposed to take them there from Morrissey , British Columbia , failed to pick them up . The Spokane Flyer , a passenger train heading west from Lethbridge , was saved by CPR brakeman Sid Choquette , one of two men who rushed across the rock @-@ strewn ground to warn the train that the track had been buried under the slide . Through falling rocks and a dust cloud that impaired his visibility , Choquette ran for 2 kilometres ( 1 @.@ 2 mi ) to warn the oncoming locomotive of the danger . The CPR gave him a letter of commendation and a $ 25 cheque in recognition of his heroism .


Turtle Mountain stands immediately south of Frank . It consists of an older limestone layer folded over on top of softer materials such as shale and sandstone . Erosion had left the mountain with a steep overhang of its limestone layer . It has long been unstable ; the Blackfoot and Kutenai peoples called it " the mountain that moves " and refused to camp in its vicinity . In the weeks leading up to the disaster , miners occasionally felt rumblings from within the mountain , while the pressure created by the shifting rock sometimes caused the timbers supporting the mine shafts to crack and splinter .


Gilbert , producers Broccoli and Harry Saltzman , production designer Ken Adam and director of photography Freddie Young then went to Japan , spending three weeks searching for locations . SPECTRE ’ s shore fortress headquarters was changed to an extinct volcano after the team learned that the Japanese do not build castles by the sea . The group was due to return to the UK on a BOAC Boeing 707 flight ( BOAC Flight 911 ) on 5 March 1966 , but cancelled after being told they had a chance to watch a ninja demonstration . That flight crashed 25 minutes after takeoff , killing all on board . In Tokyo , the crew also found Hunt , who decided to go on holiday after having his request to direct declined . Hunt was invited to direct the second unit for You Only Live Twice and accepted the job .


The railway was repaired within three weeks and the mine was quickly reopened . The section of town closest to the mountain was relocated in 1911 amid fears that another slide was possible . The town 's population nearly doubled its pre @-@ slide population by 1906 , but dwindled after the mine closed permanently in 1917 . The community is now part of the Municipality of Crowsnest Pass in the Province of Alberta and has a population around 200 . The site of the disaster , which remains nearly unchanged since the slide , is now a popular tourist destination . It has been designated a Provincial Historic Site of Alberta and is home to an interpretive centre that receives over 100 @,@ 000 visitors annually .


Mullen and The Edge both agreed that playing the Sarajevo concert had been the highlight of their careers ; Mullen said , " [ t ] here 's no doubt that that is an experience I will never forget for the rest of my life . And if I had to spend 20 years in the band just to play that show , [ ... ] I think it would have been worthwhile . " Bono described it as " one of the toughest and one of the sweetest nights of my life " , saying of the audience , " I think they wanted , more than anything , a return to normalcy . That 's what these people want , it 's what they deserve . " He also speculated that the loss of his voice had " allowed room for Sarajevo to take the gig away from us . They could see that things could go horribly wrong , they 'd gone to a lot of trouble to come here , and they were just going to make it happen . And they did . " After the completion of the PopMart Tour , he said , " it was amazing and confounding to discover that on our most ' pop ' of tours some of the best shows were in political hotspots like Santiago , Sarajevo , Tel Aviv [ ... ] anywhere music meant more than entertainment " . Following the concert , President Izetbegović presented Bono with an honorary Bosnian passport , in recognition of his humanitarian efforts during the war .


Initial reports on the disaster indicated that Frank had been " nearly wiped out " by the mountain 's collapse . It was thought the rockslide was triggered by an earthquake , volcanic eruption or explosion within the mine . The majority of the town survived , but the slide buried buildings on the eastern outskirts of Frank . Seven cottages were destroyed , as were several businesses , the cemetery , a 2 @-@ kilometre ( 1 @.@ 2 mi ) stretch of road and railroad tracks , and all of the mine 's buildings .


Three opening acts played before U2 , beginning with the Gazi Huzrev @-@ Beg choir , an Islamic choir from a local high school . Their performance was followed by two local bands , Protest and Sikter , one of which was chosen personally by Sacirbey , and the other which was selected through a radio contest . Following the opening acts , musician Howie B performed a DJ set before U2 took the stage .


Tertiary structure : the overall shape of a single protein molecule ; the spatial relationship of the secondary structures to one another . Tertiary structure is generally stabilized by nonlocal interactions , most commonly the formation of a hydrophobic core , but also through salt bridges , hydrogen bonds , disulfide bonds , and even posttranslational modifications . The term " tertiary structure " is often used as synonymous with the term fold . The tertiary structure is what controls the basic function of the protein .


On the evening of 22 November 1914 , the Grand Fleet conducted a fruitless sweep in the southern half of the North Sea to support Vice Admiral David Beatty 's 1st Battlecruiser Squadron . The fleet was back in port in Scapa Flow by 27 November . Marlborough and most of the fleet initially remained in port during the German raid on Scarborough , Hartlepool and Whitby on 16 December 1914 , though the 3rd Battle Squadron was sent to reinforce the British forces in the area . After receiving further information about the possibility of the rest of the German fleet being at sea , Jellicoe gave the order for the fleet to sortie to try to intercept the Germans , though by that time they had already retreated . Vice Admiral Cecil Burney replaced Bayley aboard Marlborough in December ; at that time , Marlborough became the second @-@ in @-@ command flagship for the Grand Fleet . On 25 December , the fleet sortied for a sweep in the North Sea , which concluded on 27 December without event . Marlborough and the rest of the fleet conducted gunnery drills during 10 – 13 January 1915 west of the Orkneys and Shetlands . On the evening of 23 January , the bulk of the Grand Fleet sailed in support of Beatty 's Battlecruiser Fleet but the rest of the fleet did not become engaged in the ensuing Battle of Dogger Bank the following day .


Over the next week the 766th Regiment and the KPA 's 5th Division continued in a slow advance south as it met increasing South Korean resistance . United Nations air support began to increase , slowing the advance further . The force continued to occupy the eastern flank , and by July 24 it was advancing from the Chongsong @-@ Andong region and approaching Pohang . On its flank was the KPA 's 12th Division . Progress halted as UN aerial and naval bombardment made movement more difficult . At the same time the North Korean units ' supply lines were stretched thin and began to break down , forcing them to conscript South Korean civilians to carry supplies .


As the Bosnian War ended in 1995 and the Siege of Sarajevo in 1996 , the stability of the region began to improve . Realizing this , U2 began to plan a concert for Sarajevo that would take place on their 1997 PopMart Tour . Although they were the first major musical artist to perform in the city following the war , China Drum had played a concert in July 1996 . Music journalist Andrew Mueller described China Drum 's experience in a single van as a " logistical and administrative nightmare " . Muhamed Sacirbey , the Bosnian Ambassador to the United Nations , helped U2 make arrangements , playing an informal role as promoter and organizer . McGuinness said , " We thought it was going to be quite difficult . But it 's been quite straightforward . People have just wanted to help . We 've blagged a lot of equipment , forklifts and so on , from the military , and the local crew have been incredibly supportive . "


Filming of You Only Live Twice lasted from July 1966 to March 1967 . The film was shot primarily in Japan . Himeji Castle in Hyōgo was depicted as Tanaka 's ninja training camp . His private transportation hub was filmed at the Tokyo Metro 's Nakano @-@ shimbashi Station . As of 2011 , many of the fixtures in the station are unchanged from the time of filming .


On August 3 , an extratropical cyclone developed into a tropical depression off the west coast of Florida . Initially a weak disturbance , it moved generally westward , slowly gaining in intensity . Early on August 4 , the depression attained tropical storm intensity . Ships in the vicinity of the storm reported a much stronger tropical cyclone than initially suggested . After reaching hurricane strength on August 5 south of the Mississippi River Delta , the storm strengthened further into a modern @-@ day Category 2 hurricane , with maximum sustained winds of 100 mph and a minimum barometric pressure of 972 mbar ( hPa ; 28 @.@ 71 inHg ) at 0600 UTC on August 7 . The hurricane moved ashore near Sabine Pass , Texas later that day at peak strength . Once inland , the storm executed a sharp curve to the north and quickly weakened , degenerating into a tropical storm on August 8 before dissipating over Arkansas on August 10 .


The 766th Independent Infantry Regiment ( Korean : 제766독립보병연대 ) was a light infantry unit of North Korea 's Korean People 's Army ( KPA ) that existed briefly during the Korean War . It was headquartered in Hoeryong , North Korea , and was also known as the 766th Unit ( Korean : 766부대 ) . Trained extensively in amphibious warfare and unconventional warfare , the 766th Regiment was considered a special forces commando unit . The regiment was trained to conduct assaults by sea and then to lead other North Korean units on offensive operations , to infiltrate behind enemy lines and to disrupt enemy supplies and communications .


The ROK 23rd Regiment of the ROK 3rd Division was moved to block the advance of the three units at Uljin . The ROK forces mounted a series of delaying actions against the main North Korean force , which was significantly dispersed throughout the mountainous region and unable to muster its overwhelming strength . The ROK regiment was subsequently able to hold up the North Korean advance until July 5 . On July 10 , the 766th separated from the 5th Division and met an advance party of North Korean civilians in Uljin who had been sent to set up government in the area . From here , the 766th dispersed in small groups into the mountains . On July 13 it reached Pyonghae @-@ ri , 25 miles ( 40 km ) north of Yongdok .


" Nellie 's " battle with helicopters proved to be difficult to film . The scenes were initially shot in Miyazaki , first with takes of the gyrocopter , with more than 85 take @-@ offs , 5 hours of flight and Wallis nearly crashing into the camera several times . A scene filming the helicopters from above created a major downdraft and cameraman John Jordan 's foot was severed by the craft 's rotor . The concluding shots involved explosions , which the Japanese government did not allow in a national park . So , the crew moved to Torremolinos , Spain , which was found to resemble the Japanese landscape .


Structural proteins confer stiffness and rigidity to otherwise @-@ fluid biological components . Most structural proteins are fibrous proteins ; for example , collagen and elastin are critical components of connective tissue such as cartilage , and keratin is found in hard or filamentous structures such as hair , nails , feathers , hooves , and some animal shells . Some globular proteins can also play structural functions , for example , actin and tubulin are globular and soluble as monomers , but polymerize to form long , stiff fibers that make up the cytoskeleton , which allows the cell to maintain its shape and size .


Several factors led to the Frank Slide . A study conducted by the GSC immediately following the slide concluded that the primary cause was the mountain 's unstable anticline formation ; a layer of limestone rested on top of softer materials that , after years of erosion , resulted in a top @-@ heavy , steep cliff . Cracks laced the eastern face of the mountain while underground fissures allowed water to flow into the mountain 's core . Local Indigenous peoples of the area , the Blackfoot and Ktunaxa , had oral traditions referring to the peak as " the mountain that moves . " Miners noticed the mountain had become increasingly unstable in the months preceding the slide ; they felt small tremors and the superintendent reported a " general squeeze " in the mountain at depths between 1 @,@ 100 metres ( 3 @,@ 600 ft ) and 1 @,@ 500 metres ( 4 @,@ 900 ft ) . They found that coal broke from its seam ; it was said to have practically mined itself .


The band first became involved with Sarajevo in 1993 on their Zoo TV Tour ; approached by aid worker Bill Carter about bringing attention to the Siege of Sarajevo , the band conducted nightly satellite transmissions with Bosnians during their shows . These link @-@ ups were the subject of criticism from journalists for mixing entertainment with human tragedy . Although the war made it impractical for U2 to visit Sarajevo at the time , they vowed to eventually play a concert in the city . After the conflict ended in November 1995 , they made arrangements to visit Sarajevo , and with help from United Nations ambassadors and peacekeeping troops , they scheduled and played the concert in 1997 .


Marlborough served with the Grand Fleet for the duration of the war , primarily patrolling the northern end of the North Sea to enforce the blockade of Germany . She saw action at the Battle of Jutland ( 31 May – 1 June 1916 ) , where she administered the coup de grâce to the badly damaged German cruiser SMS Wiesbaden . During the engagement , Wiesbaden hit Marlborough with a torpedo that eventually forced her to withdraw . The damage to Marlborough was repaired by early August , though the last two years of the war were uneventful , as the British and German fleets adopted more cautious strategies due to the threat of underwater weapons .


During the planning for the invasion of South Korea in the years before the war , the North Korean leadership began to create large numbers of commando and special forces units to send south . These units subverted South Korean authority before and during the war with terror campaigns , sabotage and inducing rebellions in ROK military units . Hundreds of commandos were sent to South Korea in this fashion , and by the end of the war up to 3 @,@ 000 of them had been trained and armed . During this time , North Korean leadership also ordered the creation of large conventional units to act as advance forces for the actual invasion . The 766th Unit was formed in April 1949 at the Third Military Academy in Hoeryong , North Korea . The academy was specially designed to train commandos , and the 766th was originally designed to supervise North Korean light infantry ranger units . Over the next year , the 766th Unit received extensive training in unconventional warfare and amphibious warfare . During this time , the unit was expanded in size to 3 @,@ 000 men in six battalions .


Sister Wives drew national media attention after its first season and garnered generally mixed reviews from critics . Washington Post staff writer Hank Stuever called it " refreshingly frank " and found most interesting the small details of the family 's everyday life , such as the food supply , division of labor , and minor arguments . Los Angeles Times television critic Mary McNamara said she was intrigued by the matriarchal nature of the polygamist family , a unit that is traditionally considered patriarchal . McNamara said the wives form the center of the family and that " their bonds appear far stronger and more vital than the casual fondness with which they all treat Kody " . Salon.com writer Schuyler Velasco praised Sister Wives for introducing viewers to unfamiliar subject matter and called it " refreshingly modest " considering its controversial subject matter . Velasco said it has " a natural , honest presence in a genre fabled for the camera @-@ hogging antics of Jersey Shore " . Shelley Fralic of The Vancouver Sun called it fascinating and surprising and was impressed with the sensible and articulate way in which the family defended their lifestyle . When the Brown family made an October 2010 appearance on The Oprah Winfrey Show , talk show host Oprah Winfrey said she found particularly fascinating the relationship between the sister wives .


The show follows the lives of advertising salesman Kody Brown , his wives Meri , Janelle , Christine , and Robyn , and their 18 children . In the first season the show televised Brown 's courting and marriage of his fourth wife , Robyn Sullivan , in 2010 . Sullivan was the first new wife to enter the family in 16 years .


LiSA released her first full solo album Lover " s " mile on February 22 , 2012 ; the album peaked at No. 7 on the Oricon weekly charts . She attended the Anime Expo 2012 in Los Angeles as a guest of honor and performed her first concert in North America there on July 1 . After her success with " Oath Sign " , she was chosen to perform the first opening theme to the 2012 anime series Sword Art Online ; the single " Crossing Field " , her second single , was released on August 8 , 2012 ; the single peaked at No. 5 on Oricon and was later certified gold by the RIAJ . Her third single " Best Day , Best Way " , which peaked at No. 6 on Oricon , was released on April 3 , 2013 , and her fourth single " Träumerei " , which peaked at No. 15 on Oricon and was used as the opening theme to the 2013 anime series Day Break Illusion , was released on August 7 , 2013 . LiSA released her second solo album Landspace on October 30 , 2013 ; the album peaked at No. 2 on Oricon charts . A photobook titled Kyō mo Ii Hi da ( 今日もいい日だっ , " Another Great Day " ) that contains photos of her taken by Lis Ani ! magazine over three years was published on November 22 , 2013 . When asked if she would revisit the meaning of her acronym in the future in an interview with Nihongogo at Anime Festival Asia 2013 in Singapore , she suggested Lovely international Super Apple .


They have a fierce local rivalry with Birmingham City . The Second City derby between Aston Villa and Birmingham City has been played since 1879 . The club 's traditional kit colours are claret shirts with sky blue sleeves , white shorts and sky blue socks . Their traditional badge is of a rampant lion , which was introduced by the club 's Scottish chairman William McGregor in honour of the Royal Standard of Scotland .


A different title song was originally recorded by Julie Rogers , but eventually discarded . Only two lines from that version were kept in the final lyrics , and the orchestral part was changed to fit Nancy Sinatra 's vocal range . Rogers ' version only appeared in a James Bond 30th Anniversary CD , with no singer credit . In the 1990s , an alternative example of a possible theme song ( also called " You Only Live Twice " and sung by Lorraine Chandler ) was discovered in the vaults of RCA Records . It became a very popular track with followers of the Northern Soul scene ( Chandler was well known for her high @-@ quality soul output on RCA ) and can be found on several RCA soul compilations .


Pattycake 's birth caught the attention of the city and brought crowds of thousands of New Yorkers to the Central Park Zoo . Reporter N. R. Kleinfield called her a child star whose " furry face served as a bit of a respite at a time when the city found itself grappling with high crime rates and an intensifying financial crisis . " Six months after Pattycake was born , the director of the zoo estimated that based on the crowds , she might draw an additional 500 @,@ 000 visitors by the time of her first birthday .


On 14 August 2006 , it was confirmed that Randy Lerner , then owner of the National Football League 's Cleveland Browns , had reached an agreement of £ 62 @.@ 6 million with Aston Villa for a takeover of the club . A statement released on 25 August to the LSE announced that Lerner had secured 59 @.@ 69 % of Villa shares , making him the majority shareholder . He also appointed himself Chairman of the club . In Ellis 's last year in charge Villa lost £ 8.2m before tax , compared with a £ 3m profit the previous year , and income had fallen from £ 51.6m to £ 49m . Lerner took full control on 18 September , as he had 89 @.@ 69 % of the shares . On 19 September 2006 , Ellis and his board resigned to be replaced with a new board headed by Lerner . Lerner installed Charles Krulak as a non @-@ executive director and Ellis was awarded the honorary position of Chairman Emeritus .


Since 2015 Villa 's shirt sponsors have been Quickbooks . Previous commercial sponsors have been Davenports ( 1982 – 83 ) , Mita ( 1983 – 93 ) , Müller ( 1993 – 95 ) , AST Computer ( 1995 – 98 ) , LDV ( 1998 – 2000 ) , NTL ( 2000 – 02 ) , Rover ( 2002 – 04 ) , DWS Investments ( 2004 – 06 ) , 32Red.com ( 2006 – 08 ) , FxPro ( 2010 – 11 ) , Genting Casinos ( 2011 – 13 ) , Dafabet ( 2013 – 2015 ) , and Intuit QuickBooks ( 2015 – ) . Since 2016 , kit has been manufactured by Under Armour . Previous manufacturers have been Umbro ( 1972 – 81 , 1990 – 93 ) , le Coq Sportif ( 1981 – 83 ) , Henson ( 1983 – 87 ) , Hummel ( 1987 – 90 , 2004 – 07 ) , Asics ( 1993 – 95 ) , Reebok ( 1995 – 2000 ) , Diadora ( 2000 – 04 ) , Nike ( 2007 – 12 ) and Macron ( 2012 @-@ 16 ) .


Secondary structure : regularly repeating local structures stabilized by hydrogen bonds . The most common examples are the α @-@ helix , β @-@ sheet and turns . Because secondary structures are local , many regions of different secondary structure can be present in the same protein molecule .


Oribe 's musical experience began at the age of three when , upon her mother 's suggestions , she took private piano lessons . Although she was a shy student in kindergarten , she enjoyed her music lessons . In primary school , she was inspired to sing after seeing the band Speed on television . She later took dance and vocal lessons , which continued through her junior high school years . While in elementary , she participated in an audition held at the Nippon Budokan , and it was during this time she decided to become an artist . In junior high school , she formed a band which covered songs by Avril Lavigne , Love Psychedelico , and Ego @-@ Wrappin ' .


The day after the concert in Sarajevo , a local newspaper carried an editorial which was headlined , " Today was the day the siege of Sarajevo ended " . In reaction to the event , a Bosnian student told members of the international press , " I felt excluded from the world for so long . It 's not only about U2 . It 's the feeling of being part of the world . " A local resident said that the concert was " proof that we have peace here , that everything is OK " . Fans from outside the former Yugoslavia described Sarajevo as " an oasis of light " in the midst of destroyed and fire @-@ damaged buildings , and deserted villages . Despite the subpar performance , the Associated Press said , " For two magical hours , the rock band U2 achieved what warriors , politicians and diplomats could not : They united Bosnia . " Andrew Mueller of The Independent wrote , " For the first time since the start of the war in 1992 , people more accustomed to seeing each other through the sights of a rifle were converging on the capital to listen to music together . It was a reminder of prewar Sarajevo , home to some of old Yugoslavia 's best rock bands . " Sacirbey stated that he was satisfied that the concert " was held to promote a sense of normalcy , peace and reconciliation in Sarajevo and not to raise money . " He also expressed thanks on behalf of President Alija Izetbegović , who described the concert as a " landmark event " .


Aston Villa have a large fanbase and draw support from all over the Midlands and beyond , with supporters ' clubs all across the world . Former Villa chief executive Richard Fitzgerald has stated that the ethnicity of the supporters is currently 98 % white . When Randy Lerner 's regime took over at Villa Park , they aimed to improve their support from ethnic minorities . A number of organisations have been set up to support the local community including Aston Pride . A Villa in the Community programme has also been set up to encourage support among young people in the region . The new owners have also initiated several surveys aimed at gaining the opinions of Villa fans and to involve them in the decision making process . Meetings also occur every three months where supporters are invited by ballot and are invited to ask questions to the Board . In 2011 , the club supported a supporter @-@ based initiative for an official anthem to boost the atmosphere at Villa Park . The song " The Bells Are Ringing " is to be played before games .


Bird One closes in on the American space capsule , and US forces prepare to launch a nuclear attack on the USSR . Meanwhile , the Japanese ninjas approach the base 's entrance , but are detected and fired upon . Bond manages to open the hatch , letting in the ninjas . During the ensuing battle , Bond fights his way to the control room and activates Bird One 's self @-@ destruct before it reaches the American craft . The Americans stand down their forces .


On Her Majesty 's Secret Service was the intended next film , but the producers decided to adapt You Only Live Twice instead because OHMSS would require searching for high and snowy locations . Lewis Gilbert originally declined the offer to direct , but accepted after producer Albert R. Broccoli called him saying : " You can 't give up this job . It 's the largest audience in the world . " Peter R. Hunt , who edited the first five Bond films , believed that Gilbert had been contracted by the producers for other work but they found they had to use him .


Between November 27 , 2006 , and April 1 , 2007 , 3329 images of the star WASP @-@ 13 by the SuperWASP @-@ North program based at Roque de los Muchachos Observatory in the Canary Islands led to the identification of WASP @-@ 13 as host to a potentially transiting object . Photometric follow @-@ up observations were taken on February 16 , 2008 using the James Gregory Telescope ( JGT ) in Scotland , which took 1047 exposures of the star , although the last twenty images taken were obscured by cloud cover and were discarded . Using HD 80408 as a reference star along with JGT measurements , the astronomers investigating the system were able to create a light curve for the transiting planet .


Other possibilities exist , as well . For example , immunohistochemistry usually utilizes an antibody to one or more proteins of interest that are conjugated to enzymes yielding either luminescent or chromogenic signals that can be compared between samples , allowing for localization information . Another applicable technique is cofractionation in sucrose ( or other material ) gradients using isopycnic centrifugation . While this technique does not prove colocalization of a compartment of known density and the protein of interest , it does increase the likelihood , and is more amenable to large @-@ scale studies .


The chocolate colour later became claret . Nobody is quite sure why claret and blue became the club 's adopted colours . Several other English football teams adopted their colours ; clubs that wear claret and blue include West Ham United and Burnley .


Pattycake was the " child star " of New York City in the early 1970s , and her fame was compared to Shirley Temple . At a time when New York City was facing many problems , she distracted the public from their growing anxieties and became a welcome relief for New Yorkers and their children who loved to visit her . After spending the first decade of her life at the Central Park Zoo , Pattycake moved permanently to the Bronx Zoo in 1982 . She was the mother of ten baby gorillas , including twins born in 1995 . Pattycake spent her later life as an independent but caring troop matriarch in the Bronx Zoo 's Congo Gorilla Forest exhibit . After suffering from arthritis and cardiac problems for some time , Pattycake succumbed to heart disease in 2013 .


Many ligand transport proteins bind particular small biomolecules and transport them to other locations in the body of a multicellular organism . These proteins must have a high binding affinity when their ligand is present in high concentrations , but must also release the ligand when it is present at low concentrations in the target tissues . The canonical example of a ligand @-@ binding protein is haemoglobin , which transports oxygen from the lungs to other organs and tissues in all vertebrates and has close homologs in every biological kingdom . Lectins are sugar @-@ binding proteins which are highly specific for their sugar moieties . Lectins typically play a role in biological recognition phenomena involving cells and proteins . Receptors and hormones are highly specific binding proteins .


The only legal marriage was between Kody and his first wife Meri , until their legal divorce in September 2014 . ( He legally married fourth wife Robyn in December 2014 in order to legally adopt her three children ) . The other marriages are considered spiritual unions . As of 2015 Kody has been married to Meri for 25 years , Janelle for 22 years , Christine for 21 years , and Robyn for 5 years . Kody and Meri have a daughter named Mariah , their only child . Kody and Janelle have six children : daughters Madison and Savanah and sons Logan , Hunter , Garrison , and Gabriel . Kody and Christine have six children : daughters Aspyn , Mykelti , Gwendlyn , Ysabel , and Truely and son Paedon . Robyn had three children from her first marriage , which was monogamous : Dayton , Aurora , and Breanna . Kody legally adopted them in June 2015 . Kody and Robyn have two children : son Solomon and daughter Ariella .


Other methods for elucidating the cellular location of proteins requires the use of known compartmental markers for regions such as the ER , the Golgi , lysosomes or vacuoles , mitochondria , chloroplasts , plasma membrane , etc . With the use of fluorescently tagged versions of these markers or of antibodies to known markers , it becomes much simpler to identify the localization of a protein of interest . For example , indirect immunofluorescence will allow for fluorescence colocalization and demonstration of location . Fluorescent dyes are used to label cellular compartments for a similar purpose .


At the age of five months , Pattycake was sharing the Lion House with Lulu and Kongo at the Central Park Zoo . On March 20 , 1973 , an accident occurred that was attributed more to clumsiness than to parenting skills . Although no staff were on hand to see it directly , according to visitors , while little Pattycake was reaching out to her father through the bars of her cage , her right arm became stuck somehow when her mother Lulu pulled her away , breaking her right arm . Zoo handlers had to subdue Lulu with a tranquilizer dart to remove Pattycake and treat her injury . While experts considered the incident an accident , the media sensationalized the event . Zookeeper Veronica Nelson , who worked with Patty Cake , recalled that


Aston Villa 's arch @-@ rivals are Birmingham City , with games between the two clubs known as the Second City Derby . Historically though , West Bromwich Albion have arguably been Villa 's greatest rivals , a view highlighted in a fan survey , conducted in 2003 . The two teams contested three FA Cup finals in the late 19th century . Villa also enjoy less heated local rivalries with Wolverhampton Wanderers and Coventry City . Through the relegation of West Brom and Birmingham City , to the Football League Championship , in the 2005 – 06 season , at the start of 2006 – 07 Premiership season , Villa were the only Midlands club in that League . The nearest opposing team Villa faced during that season was Sheffield United , who played 62 miles ( 100 km ) away in South Yorkshire . For the 2010 – 11 season , West Bromwich Albion were promoted and joined Aston Villa , Wolverhampton Wanderers , and Birmingham City in the Premier League . This marked the first time that the " West Midlands ' Big Four " clubs have been in the Premier League at the same time , and the first time together in the top flight since the 1983 – 84 season . Birmingham were relegated at the end of the 2010 – 11 season , ending this period .


The town of Frank was founded in the southwestern corner of the District of Alberta , a subdivision of the Northwest Territories in 1901 . A location was chosen near the base of Turtle Mountain in the Crowsnest Pass , where coal had been discovered one year earlier . It was named after Henry Frank who , along with Samuel Gebo , owned the Canadian @-@ American Coal and Coke Company , which operated the mine that the town was created to support . The pair celebrated the founding of the town on September 10 , 1901 , with a gala opening that featured speeches from territorial leaders , sporting events , a dinner and tours of the mine and planned layout for the community . The Canadian Pacific Railway ( CPR ) ran special trains that brought over 1 @,@ 400 people from neighbouring communities to celebrate the event . By April 1903 , the permanent population had reached 600 , and the town featured a two @-@ storey school and four hotels .


Initial news reports stated that between 50 and 60 men were within the mountain and had been buried with no hope of survival . In reality , there were 20 miners working the night shift at the time of the disaster . Three had been outside the mine and were killed by the slide . The remaining 17 were underground . They discovered that the entrance was blocked and water from the river , which had been dammed by the slide , was coming in via a secondary tunnel . They unsuccessfully tried to dig their way through the blocked entrance before one miner suggested he knew of a seam of coal that reached the surface . Working a narrow tunnel in pairs and threes , they dug through the coal for hours as the air around them became increasingly toxic . Only three men still had enough energy to continue digging when they broke through to the surface late in the afternoon . The opening was too dangerous to escape from due to falling rocks from above . Encouraged by their success , the miners cut a new shaft that broke through under an outcropping of rock that protected them from falling debris . Thirteen hours after they were buried , all 17 men emerged from the mountain .


Proteins are not entirely rigid molecules . In addition to these levels of structure , proteins may shift between several related structures while they perform their functions . In the context of these functional rearrangements , these tertiary or quaternary structures are usually referred to as " conformations " , and transitions between them are called conformational changes . Such changes are often induced by the binding of a substrate molecule to an enzyme 's active site , or the physical region of the protein that participates in chemical catalysis . In solution proteins also undergo variation in structure through thermal vibration and the collision with other molecules .


When it came to vowel forms , Keats incorporated a pattern of alternating historically " short " and " long " vowel sounds in his ode . In particular , line 18 ( " And purple @-@ stained mouth " ) has the historical pattern of " short " followed by " long " followed by " short " and followed by " long " . This alteration is continued in longer lines , including line 31 ( " Away ! away ! for I will fly to thee " ) which contains five pairs of alternations . However , other lines , such as line 3 ( " Or emptied some dull opiate to the drains " ) rely on a pattern of five " short " vowels followed by " long " and " short " vowel pairings until they end with a " long " vowel . These are not the only combination patterns present , and there are patterns of two " short " vowels followed by a " long " vowel in other lines , including 12 , 22 , and 59 , which are repeated twice and then followed up with two sets of " short " and then " long " vowel pairs . This reliance on vowel sounds is not unique to this ode , but is common to Keats 's other 1819 odes and his Eve of St. Agnes .


There are two versions of the song " You Only Live Twice " , sung by Nancy Sinatra , one directly from the movie soundtrack , and a second one for record release arranged by Billy Strange . The movie soundtrack song is widely recognised for its striking opening bars and oriental flavour , and was far more popular on radio . The record release made No. 44 on the Billboard charts in the USA , No. 11 in UK . Both versions of the title song are available on CD .


In 2006 , Aston Villa announced the creation of an " Aston Villa Hall of Fame . " This was voted for by fans and the inaugural induction saw 12 former players , managers and directors named . In May 2013 it was announced that former Villa and Bulgaria captain , Stiliyan Petrov , would be the 13th addition to the Hall of Fame .


Pattycake was brought to New York Medical College for surgery and she was given a cast for her arm . Due to concerns that Lulu would try to remove Pattycake 's cast , she was separated from her mother and moved to the Bronx Zoo for convalescence . Pattycake was treated by veterinarian Emil Dolensek who later replaced her cast with a sling . After an examination , the staff discovered that Pattycake had intestinal parasites and determined she was underfed . They also believed that as a result of the incident , Lulu wasn 't capable as a mother .


Early on April 30 a special train from Fort Macleod arrived with police officers and doctors . Premier Frederick Haultain arrived at the disaster site on May 1 , where he met with engineers who had investigated the top of Turtle Mountain . Though new fissures had formed at the peak , they felt there was limited further risk to the town ; the CPR 's chief engineer was convinced that Frank was in imminent danger from another slide . Siding with the latter , Haultain ordered the town evacuated , and the Geological Survey of Canada ( GSC ) sent two of its top geologists to investigate further . They reported that the slide had created two new peaks on the mountain and that the north peak , overlooking the town , was not in imminent danger of collapse . As a result , the evacuation order was lifted on May 10 and Frank 's citizens returned . The North @-@ West Mounted Police , reinforced by officers who arrived from Cranbrook , Macleod and Calgary , kept tight control of the town and ensured that no cases of looting occurred during the evacuation .


The chief characteristic of proteins that also allows their diverse set of functions is their ability to bind other molecules specifically and tightly . The region of the protein responsible for binding another molecule is known as the binding site and is often a depression or " pocket " on the molecular surface . This binding ability is mediated by the tertiary structure of the protein , which defines the binding site pocket , and by the chemical properties of the surrounding amino acids ' side chains . Protein binding can be extraordinarily tight and specific ; for example , the ribonuclease inhibitor protein binds to human angiogenin with a sub @-@ femtomolar dissociation constant ( 1 M ) . Extremely minor chemical changes such as the addition of a single methyl group to a binding partner can sometimes suffice to nearly eliminate binding ; for example , the aminoacyl tRNA synthetase specific to the amino acid valine discriminates against the very similar side chain of the amino acid isoleucine .


By this time , however , North Korean logistics had been stretched to their limit , and resupply became increasingly difficult . By the beginning of August , the North Korean units operating in the area were getting little to no food and ammunition supply , instead relying on captured UN weapons and foraging for what they could find . They were also exhausted from over a month of advancing , though morale remained high among the 766th troops . The 766th Regiment specialized in raiding UN supply lines , and effectively mounted small disruptive attacks against UN targets to equip themselves .


The activities and structures of proteins may be examined in vitro , in vivo , and in silico . In vitro studies of purified proteins in controlled environments are useful for learning how a protein carries out its function : for example , enzyme kinetics studies explore the chemical mechanism of an enzyme 's catalytic activity and its relative affinity for various possible substrate molecules . By contrast , in vivo experiments can provide information about the physiological role of a protein in the context of a cell or even a whole organism . In silico studies use computational methods to study proteins .


Most microorganisms and plants can biosynthesize all 20 standard amino acids , while animals ( including humans ) must obtain some of the amino acids from the diet . The amino acids that an organism cannot synthesize on its own are referred to as essential amino acids . Key enzymes that synthesize certain amino acids are not present in animals — such as aspartokinase , which catalyses the first step in the synthesis of lysine , methionine , and threonine from aspartate . If amino acids are present in the environment , microorganisms can conserve energy by taking up the amino acids from their surroundings and downregulating their biosynthetic pathways .


At various points during " Until the End of the World " and " New Year 's Day " , Bono gestured for the audience to help him with the vocals , and by the eighth song of the night , " Pride ( In the Name of Love ) " , he was reduced to speaking the lyrics instead of singing them . The band continued with their standard set list by playing " I Still Haven 't Found What I 'm Looking For " , " Stand By Me " , " All I Want Is You " , and " Staring at the Sun " . U2 considered playing " Desire " after " All I Want Is You " , but they chose not to perform the song . The Edge then performed a solo version of " Sunday Bloody Sunday " . The rendition was slower and quieter than the studio version . During the song , Bono went backstage for cortisone injections , which helped to improve his voice for a short time . Brian Eno was prepared to go on stage to replace Bono if he could not continue , or to sing alongside him . Bono ultimately returned to the stage alone for the next song , " Bullet the Blue Sky " , and the band continued with renditions of " Please " and " Where the Streets Have No Name " , which concluded the main set .


Her injury was treated at the Bronx Zoo while a custody dispute between the two zoos broke out in public and elicited a range of opinions from experts who believed Pattycake should be returned to her mother . Intense media coverage and public interest brought Pattycake to the attention of a wide audience , with stories focusing on her recovery , her eventual reunion with her parents , and the conditions of zoo animals in Central Park . An ambitious proposal for renovating the Central Park Zoo arose in the wake of the controversy while the zoo received record attendance rates .


In making the single " Bright Flight / L. Miranic " , she mentions in an interview with HMV Japan that the concept of the single is that it can be divided into two parts : pink and black . Pink is the color of " Bright Flight " , a song which is in a pop style and was written in a " cute " image in mind , while black is the color of " L. Miranic " , which is in a dark rock style and has the theme of a " bad woman " . LiSA mentioned that the name " L. Miranic " was chosen because the song is linked to criminals and that since " Miranic " is a name in some countries , adding her name to " Miranic " to make " LiSA @-@ Miranic " would mean that the criminal in the song is her .


Transmembrane proteins can also serve as ligand transport proteins that alter the permeability of the cell membrane to small molecules and ions . The membrane alone has a hydrophobic core through which polar or charged molecules cannot diffuse . Membrane proteins contain internal channels that allow such molecules to enter and exit the cell . Many ion channel proteins are specialized to select for only a particular ion ; for example , potassium and sodium channels often discriminate for only one of the two ions .


Importantly , the concentration of individual proteins ranges from a few molecules per cell to hundreds of thousands . In fact , about a third of all proteins is not produced in most cells or only induced under certain circumstances . For instance , of the 20 @,@ 000 or so proteins encoded by the human genome only 6 @,@ 000 are detected in lymphoblastoid cells .


Proteins ( / ˈproʊˌtiːnz / or / ˈproʊti.ᵻnz / ) are large biomolecules , or macromolecules , consisting of one or more long chains of amino acid residues . Proteins perform a vast array of functions within organisms , including catalysing metabolic reactions , DNA replication , responding to stimuli , and transporting molecules from one location to another . Proteins differ from one another primarily in their sequence of amino acids , which is dictated by the nucleotide sequence of their genes , and which usually results in protein folding into a specific three @-@ dimensional structure that determines its activity .


Dennis Amith of J ! -ENT reviewed LiSA 's album Landspace , where he describes the song " Crossing Field " as a song about " wanting to forget the weakness and securities of one past and being with someone you love who gives you the strength to become even stronger . " Meanwhile , he describes the song " Best Day , Best Way " as " a fun and happy track about believing in yourself and overcoming anything bad that have happened in the past and moving forward . " Finally , he describes " Träumerei " as " another inspirational song about one losing the rhythm in their heart and is now on the road of loneliness , but still the person wants to pierce the sky with their own light , shatter the darkness and have a fresh new start in life . " He concludes the review by saying that the album is full of upbeat and inspirational songs and showcases LiSA 's talents as a vocalist , and in contrast to many anison artists who are known for their cute and sweet vocals , LiSA is able to take on many musical styles .


IGN ranked You Only Live Twice as the fourth best Bond film , and Entertainment Weekly as the second best , considering that it " pushes the series to the outer edge of coolness " . But Norman Wilner of MSN chose it as the fifth worst , criticising the plot , action scenes and little screentime for Blofeld . Literary critic Paul Simpson called the film one of the most colourful of the series and credited the prefecture of Kagoshima for adding " a good flavour " of Japanese influence on the film , but he panned the depiction of Blofeld as a " let @-@ down " , " small , bald and a whooping scar . " Simon Winder said that the film is " perfect " for parodies of the series .


The slide created two new peaks on the mountain ; the south peak stands 2 @,@ 200 metres ( 7 @,@ 200 ft ) high and the north peak 2 @,@ 100 metres ( 6 @,@ 900 ft ) . Geologists believe that another slide is inevitable , though not imminent . The south peak is considered the most likely to fall ; it would likely create a slide about one @-@ sixth the size of the 1903 slide . The mountain , continuously monitored for changes in stability , has been studied on numerous occasions . The Alberta Geological Survey operates a state @-@ of @-@ the @-@ art monitoring system used by researchers around the world . Over 80 monitoring stations have been placed on the face of the mountain to provide an early warning system for area residents in case of another slide .


The development of such tools has been driven by the large amount of genomic and proteomic data available for a variety of organisms , including the human genome . It is simply impossible to study all proteins experimentally , hence only a few are subjected to laboratory experiments while computational tools are used to extrapolate to similar proteins . Such homologous proteins can be efficiently identified in distantly related organisms by sequence alignment . Genome and gene sequences can be searched by a variety of tools for certain properties . Sequence profiling tools can find restriction enzyme sites , open reading frames in nucleotide sequences , and predict secondary structures . Phylogenetic trees can be constructed and evolutionary hypotheses developed using special software like ClustalW regarding the ancestry of modern organisms and the genes they express . The field of bioinformatics is now indispensable for the analysis of genes and proteins .


The nine @-@ episode first season ran from September 26 to November 21 , 2010 . The season premiere introduced viewers to Kody Brown and his three wives , Meri , Janelle , and Christine , and their twelve children , all of whom lived in a ranch @-@ style home with three interconnected apartments . It also chronicled Kody 's dating and engagement to Robyn Sullivan , who herself has three children , marking the first time in 16 years Kody had courted another wife . The new relationship creates insecurity and jealousy among the other three wives , but they ultimately accept her and welcome her into the family . During the fourth episode of the season , Christine gives birth to her sixth child , Truely , which brings the family to 16 children including Robyn 's three kids .


The peptide bond has two resonance forms that contribute some double @-@ bond character and inhibit rotation around its axis , so that the alpha carbons are roughly coplanar . The other two dihedral angles in the peptide bond determine the local shape assumed by the protein backbone . The end of the protein with a free carboxyl group is known as the C @-@ terminus or carboxy terminus , whereas the end with a free amino group is known as the N @-@ terminus or amino terminus . The words protein , polypeptide , and peptide are a little ambiguous and can overlap in meaning . Protein is generally used to refer to the complete biological molecule in a stable conformation , whereas peptide is generally reserved for a short amino acid oligomers often lacking a stable three @-@ dimensional structure . However , the boundary between the two is not well defined and usually lies near 20 – 30 residues . Polypeptide can refer to any single linear chain of amino acids , usually regardless of length , but often implies an absence of a defined conformation .


Finally , the gold @-@ standard method of cellular localization is immunoelectron microscopy . This technique also uses an antibody to the protein of interest , along with classical electron microscopy techniques . The sample is prepared for normal electron microscopic examination , and then treated with an antibody to the protein of interest that is conjugated to an extremely electro @-@ dense material , usually gold . This allows for the localization of both ultrastructural details as well as the protein of interest .


Three Aston Villa players have won the PFA Players ' Player of the Year award . At the end of every English football season , the members of the Professional Footballers ' Association ( PFA ) vote on which of its members has played the best football in the previous year . In 1977 Andy Gray won the award . In 1990 it was awarded to David Platt , whilst Paul McGrath won it in 1993 . The PFA Young Player of the Year , which is awarded to players under the age of 23 , has been awarded to four players from Aston Villa : Andy Gray in 1977 ; Gary Shaw in 1981 ; Ashley Young in 2009 and James Milner in 2010 . The National Football Museum in Preston , Lancashire administers the English Football Hall of Fame which currently contains two Villa teams , two Villa players and one manager . The 1890s team and 1982 team were inducted into the Hall of Fame in July 2009 . Joe Mercer was inducted into the Hall of Fame at the same time for his career as a manager including his time at Aston Villa . The only two Villa players in the Hall of Fame are Danny Blanchflower and Peter Schmeichel .


Marlborough was 622 feet 9 inches ( 190 m ) long overall and had a beam of 90 ft ( 27 m ) and an average draught of 29 ft 6 in ( 9 m ) . She displaced 25 @,@ 000 long tons ( 25 @,@ 401 t ) as designed and up to 29 @,@ 560 long tons ( 30 @,@ 034 t ) at combat loading . Her propulsion system consisted of four Parsons steam turbines , with steam provided by eighteen Babcock & Wilcox boilers . The engines were rated at 29 @,@ 000 shaft horsepower ( 21 @,@ 625 kW ) and produced a top speed of 21 @.@ 25 kn ( 39 km / h ; 24 mph ) . Marlborough 's cruising radius was 7 @,@ 800 nautical miles ( 14 @,@ 446 km ; 8 @,@ 976 mi ) at a more economical 10 kn ( 19 km / h ; 12 mph ) . She had a crew of 995 officers and enlisted men ; during wartime this increased to up to 1 @,@ 022 .


The molecules bound and acted upon by enzymes are called substrates . Although enzymes can consist of hundreds of amino acids , it is usually only a small fraction of the residues that come in contact with the substrate , and an even smaller fraction — three to four residues on average — that are directly involved in catalysis . The region of the enzyme that binds the substrate and contains the catalytic residues is known as the active site .


Several hundred members of the international " Stabilisation Force " ( SFOR ) were tasked with upholding the Dayton Agreement for the concert . The band were overwhelmed by the sights they saw when arriving . During the war , Koševo Stadium was used as a morgue , and graveyards were present on either sides . Although the venue had escaped the worst of the shelling , the nearby Olympic Hall Zetra had been badly damaged during the war . Despite its condition , U2 used the building for their dressing rooms and offices . Following the concert , it was used to provide lodging for 3 @,@ 000 fans . The band 's hotel , a nearby Holiday Inn , had been shelled during the siege , and part of the building had been destroyed as a result . The walls in Mullen 's room were punctured with mortar shrapnel , and sections of the floor were also missing . Prior to the show , Sacirbey took Mullen on a tour of the city , showing him the Sarajevo Roses embedded in the streets .


Most proteins consist of linear polymers built from series of up to 20 different L @-@ α @-@ amino acids . All proteinogenic amino acids possess common structural features , including an α @-@ carbon to which an amino group , a carboxyl group , and a variable side chain are bonded . Only proline differs from this basic structure as it contains an unusual ring to the N @-@ end amine group , which forces the CO – NH amide moiety into a fixed conformation . The side chains of the standard amino acids , detailed in the list of standard amino acids , have a great variety of chemical structures and properties ; it is the combined effect of all of the amino acid side chains in a protein that ultimately determines its three @-@ dimensional structure and its chemical reactivity . The amino acids in a polypeptide chain are linked by peptide bonds . Once linked in the protein chain , an individual amino acid is called a residue , and the linked series of carbon , nitrogen , and oxygen atoms are known as the main chain or protein backbone .


Unlike most James Bond films featuring various locations around the world , almost the entire film is set in one country and several minutes are devoted to an elaborate Japanese wedding . This is in keeping with Fleming 's original novel , which also devoted a number of pages to the discussion of Japanese culture . Toho Studios provided soundstages , personnel , and the female Japanese stars to the producers .


Proteins are the chief actors within the cell , said to be carrying out the duties specified by the information encoded in genes . With the exception of certain types of RNA , most other biological molecules are relatively inert elements upon which proteins act . Proteins make up half the dry weight of an Escherichia coli cell , whereas other macromolecules such as DNA and RNA make up only 3 % and 20 % , respectively . The set of proteins expressed in a particular cell or cell type is known as its proteome .


A custody dispute began between the two zoos , with the Bronx Zoo arguing that she would be better cared for in their facilities . Time magazine noted that it was the " custody battle of the decade " in the " primate world " , comparing Patty Cake 's popularity and fame to that of child star Shirley Temple . Developmental biologist Ronald Nadler of the Yerkes National Primate Research Center was brought in to arbitrate the dispute and published a report that favored returning Pattycake to her mother and the Central Park Zoo . In his report Nadler noted that " the recommendation is based on the judgment that an infant gorilla is more likely to develop into a socially competent and reproductively adequate animal if it is raised in the company of its parents as opposed to being raised with a group of peers . "


Aston Villa 's current home venue is Villa Park , which is a UEFA 5 @-@ star rated stadium , having previously played at Aston Park ( 1874 – 1876 ) and Wellington Road ( 1876 – 1897 ) . Villa Park is the largest football stadium in the English Midlands , and the eighth largest stadium in England . It has hosted 16 England internationals at senior level , the first in 1899 , and the most recent in 2005 . Thus , it was the first English ground to stage international football in three different centuries . Villa Park is the most used stadium in FA Cup semi @-@ final history , having hosted 55 semi @-@ finals . The club have planning permission to extend the North Stand ; this will involve the ' filling in ' of the corners to either side of the North Stand . If completed , the capacity of Villa Park will be increased to approximately 51 @,@ 000 .


Bono agreed to Carter 's request without asking the rest of the band , and when informed of the idea , the other members gave only tacit approval . They briefly considered playing an impromptu concert in the city , with Bono suggesting that they perform in the bunker where Carter and his friends hid during the siege . He said , " even if all we get is some extra attention for Bosnia on MTV , that 's something " . The idea fell through when it was pointed out that the logistics of transporting their equipment into the city were impossible , as the only way into Sarajevo was on a United Nations plane . Manager Paul McGuinness realized that even if the band managed to organize a concert , it would endanger their lives and those of the audience and the Zoo TV crew . As he explained , " U2 's effort to discuss any humanitarian issue have sometimes been accompanied by a false instinct that U2 is also obliged to resolve that issue . Going to Sarajevo seems to me to fall into that category . I think it would endanger the people we go with , endanger the tour , and endanger the band . " Drummer Larry Mullen , Jr. feared that the move would look like a publicity stunt .


An unusually warm winter , with warm days and cold nights , was also a factor . Water in the mountain 's fissures froze and thawed repeatedly , further weakening the mountain 's supports . Heavy snowfall in the region in March was followed by a warm April , causing the mountain snows to melt into the fissures . GSC geologists concluded that the weather conditions that night likely triggered the slide . The crew of the freight train that arrived at Frank shortly before the disaster said it was the coldest night of the winter , with overnight temperatures falling below − 18 ° C ( 0 ° F ) . Geologists speculated that the cold snap and rapid freezing resulted in expansion of the fissures , causing the limestone to break off and tumble down the mountain .


The first protein structures to be solved were hemoglobin and myoglobin , by Max Perutz and Sir John Cowdery Kendrew , respectively , in 1958 . As of 2016 , the Protein Data Bank has over 115 @,@ 000 atomic @-@ resolution structures of proteins . In more recent times , cryo @-@ electron microscopy of large macromolecular assemblies and computational protein structure prediction of small protein domains are two methods approaching atomic resolution .


" You 're Gonna Love Tomorrow " takes place five years after the aforementioned events . Important plot information that takes place during the time jump is revealed in flashbacks . Orson has completed his prison sentence and the success of Bree 's catering company has provided her the opportunity to write her own cookbook . Her business partner , Katherine Mayfair ( Dana Delany ) , resents how Bree 's success has changed her and intentionally sabotages Bree 's television interview , further straining their friendship . A flashback reveals that Danielle married a lawyer and coldly took her son away from Bree . Meanwhile , Lynette is frustrated with Tom 's ( Doug Savant ) relaxed approach to parenting their rebellious teenage sons , Porter and Preston ( Charlie Carver and Max Carver , respectively ) . She encourages him to employ more disciplinary actions .


Following a 10 @-@ year break from music , Jackson signed with her brother Michael 's record label , MJJ Music , in 1997 . From the label , Yours Faithfully was released on March 31 , 1998 . The album featured a remixed version of Jackson 's successful " Centipede " . Initially , the singer had not wanted to feature the track , believing that it was part of the past . After thinking about it for a while , Jackson felt that the inclusion of the remix — which features a rap by son Austin — would be a good way to return to the music scene . In addition , two of her other children , Stacee and Yashi , contributed backing vocals for the album . Other tracks from the album included " Fly Away " , which was written and produced by brother Michael , who also served as co @-@ executive producer for Yours Faithfully . Fellow producers included Keith Thomas and Eliot Kennedy . The album also featured a duet with Men of Vizion 's Spanky Williams on The Spinners ' " I Don 't Want to Lose You " , which Jet described as being a " sizzling " rendition . Yours Faithfully 's title track was released as a single and peaked at number 76 on the R & B chart . Vibe magazine 's Quohnos Mitchell expressed disappointment in the album , labelling its content a " mix of dated R & B grooves dressed up with a few cleverly placed samples " .


Oribe began her singing career in 2005 during her first year in high school when she formed the indie rock band Chucky , which mainly covered songs by other artists . During her second year , the band received advice from their peers that they should start making their own songs ; eventually the band 's reputation grew enough that they performed in Osaka and Nagoya in addition to Gifu . Although her grades in high school were good , she decided not to go to university , despite the recommendations of her teachers , in order to focus on her work with Chucky . Nevertheless , after graduation , due to varying schedules among the band 's members , it became difficult to continue performing . Following the band 's disbandment in July 2008 , she moved to Tokyo in order to continue her singing career .


In an interview with Oricon , LiSA explained her experiences with " Träumerei " , where she stated that because Day Break Illusion is an original anime , she wanted the song to be presented in the color of her own work , but even in the world of anime , her music would continue to have an atmosphere of rock , as opposed to the pop style she used in " Best Day , Best Way " . She mentioned that she sang that song in a way that she felt the conflicts that were present in the story and with the strength that was part of the show 's theme . With regards to the song 's music video , she wanted to have a video which would express in color emotions , such as the use of red to represent confrontations , black to represent the feeling of being lost , and green for frightening things .


In the early morning hours of April 29 , 1903 , a freight train pulled out of the mine and was slowly making its way towards the townsite when the crew heard a deafening rumble behind them . The engineer instinctively set the throttle to full speed ahead and sped his train to safety across the bridge over the Crowsnest River . At 4 : 10 am , 30 million cubic metres ( 82 million tonnes ) of limestone rock broke off the peak of Turtle Mountain . The section that broke was 1 @,@ 000 metres ( 3 @,@ 300 ft ) wide , 425 metres ( 1 @,@ 394 ft ) high and 150 metres ( 490 ft ) deep . Witnesses to the disaster claimed it took about 100 seconds for the slide to reach up the opposing hills , indicating the mass of rock traveled at a speed of about 112 kilometres per hour ( 70 mph ) . The sound was heard as far away as Cochrane , over 200 kilometres ( 120 mi ) north of Frank .


The current training ground is located at Bodymoor Heath near Kingsbury in north Warwickshire , the site for which was purchased by former chairman Doug Ellis in the early 1970s from a local farmer . Although Bodymoor Heath was state @-@ of @-@ the @-@ art in the 1970s , by the late 1990s the facilities had started to look dated . In November 2005 , Ellis and Aston Villa plc announced a state of the art GB £ 13 million redevelopment of Bodymoor in two phases . However , work on Bodymoor was suspended by Ellis due to financial problems , and was left in an unfinished state until new owner Randy Lerner made it one of his priorities to make the site one of the best in world football . The new training ground was officially unveiled on 6 May 2007 , by then manager Martin O 'Neill , then team captain Gareth Barry and 1982 European Cup winning team captain Dennis Mortimer , with the Aston Villa squad moving in for the 2007 – 08 season .


Many more gene sequences are known than protein structures . Further , the set of solved structures is biased toward proteins that can be easily subjected to the conditions required in X @-@ ray crystallography , one of the major structure determination methods . In particular , globular proteins are comparatively easy to crystallize in preparation for X @-@ ray crystallography . Membrane proteins , by contrast , are difficult to crystallize and are underrepresented in the PDB . Structural genomics initiatives have attempted to remedy these deficiencies by systematically solving representative structures of major fold classes . Protein structure prediction methods attempt to provide a means of generating a plausible structure for proteins whose structures have not been experimentally determined .


Aston Villa currently hold the record number of league goals scored by any team in the English top flight ; 128 goals were scored in the 1930 – 31 season , one more than Arsenal who won the league that season for the very first time , with Villa runners @-@ up . Villa legend Archie Hunter became the first player to score in every round of the FA Cup in Villa 's victorious 1887 campaign . Villa 's longest unbeaten home run in the FA Cup spanned 13 years and 19 games , from 1888 to 1901 .


L. indigo is distributed throughout southern and eastern North America but is most common along the Gulf Coast , Mexico , and Guatemala . Its frequency of appearance in the Appalachian Mountains of the United States has been described as " occasional to locally common " . Mycologist David Arora notes that in the United States , the species is found with ponderosa pine in Arizona , but is absent in California 's ponderosa pine forests . It has also been collected from China , India , Guatemala , and Costa Rica ( in forests dominated by oak ) . In Europe , it has so far only been found in southern France . A study on the seasonal appearance of fruiting bodies in the subtropical forests of Xalapa , Mexico , confirmed that maximal production coincided with the rainy season between June and September .


Hesler LR , Smith AH ( 1979 ) . North American Species of Lactarius . Ann Arbor , Michigan : The University of Michigan Press . ISBN 0 @-@ 472 @-@ 08440 @-@ 2 .


The Wisteria Lane set , which is located on the Colonial Street backlot set at Universal Studios , underwent changes for the time jump . Production designer P. Erik Carlson explained , " [ former production designer Thomas A. Walsh ] had already created a fairly Utopian world , and we wanted to exaggerate and enhance it a little bit more , mostly through the use of color . We didn ’ t want it to feel ridiculously futuristic or viewers would be jarred by the contrast . " Bree 's garage was remodeled into a testing kitchen as result of the character 's catering business . Additionally , all houses were repainted with bolder colors , with the exception of Gabrielle 's home , in order to reflect the Solis ' financial situation .


Proteins are assembled from amino acids using information encoded in genes . Each protein has its own unique amino acid sequence that is specified by the nucleotide sequence of the gene encoding this protein . The genetic code is a set of three @-@ nucleotide sets called codons and each three @-@ nucleotide combination designates an amino acid , for example AUG ( adenine @-@ uracil @-@ guanine ) is the code for methionine . Because DNA contains four nucleotides , the total number of possible codons is 64 ; hence , there is some redundancy in the genetic code , with some amino acids specified by more than one codon . Genes encoded in DNA are first transcribed into pre @-@ messenger RNA ( mRNA ) by proteins such as RNA polymerase . Most organisms then process the pre @-@ mRNA ( also known as a primary transcript ) using various forms of Post @-@ transcriptional modification to form the mature mRNA , which is then used as a template for protein synthesis by the ribosome . In prokaryotes the mRNA may either be used as soon as it is produced , or be bound by a ribosome after having moved away from the nucleoid . In contrast , eukaryotes make mRNA in the cell nucleus and then translocate it across the nuclear membrane into the cytoplasm , where protein synthesis then takes place . The rate of protein synthesis is higher in prokaryotes than eukaryotes and can reach up to 20 amino acids per second .


In the episode , Edie ( Nicollette Sheridan ) returns to Wisteria Lane after a five @-@ year absence with her new husband , Dave ( Neal McDonough ) . While Susan ( Teri Hatcher ) hesitates to make a romantic commitment to Jackson ( Gale Harold ) , Lynette ( Felicity Huffman ) struggles with her defiant teenage children and her husband 's midlife crisis . Gabrielle ( Eva Longoria ) deals with her daughters ' weight problems and Bree ( Marcia Cross ) faces the ramifications of her success .


LiSA made her solo debut on April 20 , 2011 with the release of her mini @-@ album Letters to U by Aniplex under Sony Music Artists . The songs on the album were composed by dōjin and major artists , and she composed the first song " Believe in Myself " ; she wrote the album 's lyrics . On November 12 , 2011 , She made her first appearance in Singapore at Anime Festival Asia , Southeast Asia 's largest anime and pop culture convention . She released her first solo single " Oath Sign " on November 23 , 2011 , which was used as the opening theme to the 2011 anime series Fate / Zero . The single peaked at No. 5 on the Oricon weekly charts and was certified gold by the Recording Industry Association of Japan ( RIAJ ) .


Aston Villa have also featured on several occasions in prose . Stanley Woolley , a character in Derek Robinson 's Booker shortlisted novel Goshawk Squadron is an Aston Villa fan and names a pre @-@ war starting eleven Villa side . Together with The Oval , Villa Park is referenced by the poet Philip Larkin in his poem about the First World War , MCMXIV . Aston Villa are also mentioned in Harold Pinter 's play The Dumb Waiter .


The characteristic blue color of the fruiting body and the latex make this species easily recognizable . Other Lactarius species with some blue color include the " silver @-@ blue milky " ( L. paradoxus ) , found in eastern North America , which has a grayish @-@ blue cap when young , but it has reddish @-@ brown to purple @-@ brown latex and gills . L. chelidonium has a yellowish to dingy yellow @-@ brown to bluish @-@ gray cap and yellowish to brown latex . L. quieticolor has blue @-@ colored flesh in the cap and orange to red @-@ orange flesh in the base of the stem . Although the blue discoloration of L. indigo is thought to be rare in the genus Lactarius , in 2007 five new species were reported from Peninsular Malaysia with bluing latex or flesh , including L. cyanescens , L. lazulinus , L. mirabilis , and two species still unnamed .


The first protein to be sequenced was insulin , by Frederick Sanger , in 1949 . Sanger correctly determined the amino acid sequence of insulin , thus conclusively demonstrating that proteins consisted of linear polymers of amino acids rather than branched chains , colloids , or cyclols . He won the Nobel Prize for this achievement in 1958 .


Villa were one of the founding members of the Premier League in 1992 , and finished runners @-@ up to Manchester United in the inaugural season . For the rest of the Nineties however Villa went through three different managers and their league positions were inconsistent , although they did win two League Cups and regularly achieved UEFA Cup qualification . Villa reached the FA Cup final in 2000 but lost 1 – 0 to Chelsea in the last game to be played at the old Wembley Stadium . Again Villa 's league position continued to fluctuate under several different managers and things came to a head in the summer of 2006 when David O 'Leary left in acrimony . After 23 years as chairman and single biggest shareholder ( approximately 38 % ) , Doug Ellis finally decided to sell his stake in Aston Villa due to ill @-@ health . After much speculation it was announced the club was to be bought by American businessman Randy Lerner , owner of NFL franchise the Cleveland Browns .


The gradual development of blue to violet pigmentation as one progresses from species to species is an interesting phenomenon deserving further study . The climax is reached in L. indigo which is blue throughout . L. chelidonium and its variety chelidonioides , L. paradoxus , and L. hemicyaneus may be considered as mileposts along the road to L. indigo .


Several casting changes were made as a result of the five @-@ year jump . Neal McDonough 's involvement was announced in July 2008 . McDonough did not audition for the role of Dave Williams , but was offered it after meeting with Cherry . Gale Harold continued appearing as Jackson , Susan 's new love interest , after appearing briefly in the flashforward segment at the end of the fourth season . The roles of the Scavo children were recast in order to reflect their new ages following the time jump . Charlie and Max Carver replaced Brent and Shane Kinsman as Preston and Porter , respectively . The Kinsman twins appeared in a flashback in this episode . Joshua Logan Moore was cast as Parker , a role previously played by Zane Huett , and Kendall Applegate joined the cast as Penny , who had previously been portrayed by several toddler actresses . Despite their casting announcements alongside the Carver twins , Moore and Applegate did not appear in " You 're Gonna Love Tomorrow " . The flashforward segment at the end of the fourth season also introduced Kaila Say and Daniella Baltodano as Gabrielle 's daughters , Juanita and Celia , respectively . Only Baltodano continued portraying her role for the fifth season ; Say was replaced by Madison De La Garza during the summer hiatus .


Desperate Housewives focuses on the lives of several residents living on Wisteria Lane . In recent episodes , Susan ( Teri Hatcher ) and Mike Delfino ( James Denton ) celebrate the birth of their son . Bree Hodge ( Marcia Cross ) chooses to raise her grandson while her daughter , Danielle ( Joy Lauren ) , attends college . She also issues an ultimatum , telling her husband , Orson ( Kyle MacLachlan ) , that she will leave him if he does not turn himself into the police after committing a hit @-@ and @-@ run . Gabrielle Solis ( Eva Longoria ) struggles with Carlos ' ( Ricardo Antonio Chavira ) permanent blindness . Also , Edie Britt ( Nicollette Sheridan ) leaves Wisteria Lane after the other residents shun her for her malice .


" Pattycake " was born on September 3 , 1972 , to western lowland gorilla parents Lulu and Kongo at the Central Park Zoo . Lulu and Kongo first arrived at the zoo on May 11 , 1966 . At the time , it was thought that Lulu wasn 't mature enough to conceive , so when Lulu gave birth to Pattycake at the age of 8 , it came as a surprise . Up until the time Lulu gave birth , it had been very difficult to get captive gorilla mothers to raise their young in zoos because the babies would either be rejected by the mother or they would be taken away by the handlers . Pattycake was the first gorilla successfully born in captivity in New York .


Antibodies are protein components of an adaptive immune system whose main function is to bind antigens , or foreign substances in the body , and target them for destruction . Antibodies can be secreted into the extracellular environment or anchored in the membranes of specialized B cells known as plasma cells . Whereas enzymes are limited in their binding affinity for their substrates by the necessity of conducting their reaction , antibodies have no such constraints . An antibody 's binding affinity to its target is extraordinarily high .


On July 11 , 1993 , Pattycake and Timmy gave birth to Okpara , a male gorilla . The pair also gave birth to twin males , Ngoma and Tambo , on August 8 , 1994 . It was the sixth time western lowland gorillas had given birth to twins in captivity . The twins were raised in a separate habitat by surrogate mothers . After seven years , Ngoma and Tambo , along with another gorilla named Dan , left for a zoo in Nebraska in February 2001 . On February 4 , 2001 , Pattycake and Zuri gave birth to Dossi , a female . Her last and tenth baby gorilla , a male , was delivered on April 15 , 2002 . Unnamed , it died four days later .


Pattycake suffered from chronic heart disease and arthritis as she aged . She was one of 338 captive zoo gorillas within North America when she died in her sleep at the age of 40 . According to the Wildlife Conservation Society , Pattycake exceeded the median life span of 37 years for female zoo gorillas .


Homer 's Mount Lushmore caricature resembles Eustace Tilley , the mascot of The New Yorker . Homer 's line about getting out of Springfield is lifted from It 's a Wonderful Life , while Homer 's all @-@ time favorite song is revealed to be " It 's Raining Men " by The Weather Girls . When Abe Simpson is left behind on the plane , his slapping his hands on his face and yelling is a reference to Home Alone . This is the second time Home Alone has been parodied on The Simpsons . The first was " Homer Alone " , a reference to the title .


After moving to Tokyo , Oribe formed the band Love is Same All with members from the indie band Parking Out and began using the stage name LiSA , which is an acronym for Love is Same All . The band performs with LiSA during the latter 's solo live performances . In 2010 , she made her major debut singing songs for the anime series Angel Beats ! as one of two vocalists for the fictional in @-@ story band Girls Dead Monster . She was the vocalist for the character Yui , and the second vocalist , Marina , sang as the character Masami Iwasawa . LiSA put out three singles and one album in 2010 under the name Girls Dead Monster on Key 's record label Key Sounds Label . The first single " Thousand Enemies " was released on May 12 ; the second single " Little Braver " came out on June 9 ; and the third single " Ichiban no Takaramono ( Yui final ver . ) " ( 一番の宝物 〜 Yui final ver . 〜 , " My Most Precious Treasure ( Yui final ver . ) " ) was sold on December 8 . The album Keep The Beats ! was released on June 30 . LiSA made her first appearance at Animelo Summer Live during the concert 's 2010 iteration on August 28 .


A number of television programmes have included references to Aston Villa over the past few decades . In the sitcom Porridge , the character Lennie Godber is a Villa supporter . When filming began on Dad 's Army , Villa fan Ian Lavender was allowed to choose Frank Pike 's scarf from an array in the BBC wardrobe ; he chose a claret and blue one — Aston Villa 's colours . The character Nessa in the BBC sitcom Gavin & Stacey was revealed as an Aston Villa fan in an episode screened in December 2009 . In the BBC series " Yes Minister " / " Yes Prime Minister " , the Minister Jim Hacker 's local team was Aston Villa , as was the Member of Parliament for Birmingham East .


In Mexico , individuals harvest the wild mushrooms for sale at farmers ' markets , typically from June to November ; they are considered a " second class " species for consumption . L. indigo is also sold in Guatemalan markets from May to October . It is one of 13 Lactarius species sold at rural markets in Yunnan in southwestern China .


Aston Villa Football Club were formed in March 1874 , by members of the Villa Cross Wesleyan Chapel in Handsworth which is now part of Birmingham . The four founders of Aston Villa were Jack Hughes , Frederick Matthews , Walter Price and William Scattergood . Aston Villa 's first match was against the local Aston Brook St Mary 's Rugby team . As a condition of the match , the Villa side had to agree to play the first half under rugby rules and the second half under football rules . After moving to the Wellington Road ground in 1876 , Villa soon established themselves as one of the best teams in the Midlands , winning their first honour , the Birmingham Senior Cup in 1880 , under the captaincy of Scotsman George Ramsay .


The First Battle of Maryang San ( 3 – 8 October 1951 ) , also known as the Defensive Battle of Maliangshan ( Chinese : 马良山防御战 ; pinyin : Mǎliáng Shān Fángyù Zhàn ) , was fought during the Korean War between United Nations ( UN ) forces — primarily Australian and British — and the Chinese communist People 's Volunteer Army . The fighting occurred during a limited UN offensive by US I Corps , codenamed Operation Commando . This offensive ultimately pushed the Chinese back from the Imjin River to the Jamestown Line and destroyed elements of four Chinese armies following heavy fighting . The much smaller battle at Maryang San took place over a five @-@ day period , and saw the 3rd Battalion , Royal Australian Regiment ( 3 RAR ) dislodge a numerically superior Chinese force from the tactically important Kowang @-@ San ( Hill 355 ) and Maryang San ( Hill 317 ) features , in conjunction with other units of the 1st Commonwealth Division .


Innis was also a central participant in an international project that produced 25 scholarly volumes between 1936 and 1945 . It was a series called The Relations of Canada and the United States overseen by James T. Shotwell , director of the Carnegie Endowment for International Peace . Innis edited and wrote prefaces for the volumes contributed by Canadian scholars . His own study of the cod fisheries also appeared as part of the series . His work with Shotwell enabled Innis to gain access to Carnegie money to further Canadian academic research . As John Watson points out , " the project offered one of the few sources of research funds in rather lean times " .


" You 're Gonna Love Tomorrow " is the fifth season premiere episode of the American comedy @-@ drama series Desperate Housewives , and the 88th episode overall . It originally aired on September 28 , 2008 , in the United States on ABC ( American Broadcasting Company ) . The episode was written by series creator Marc Cherry and directed by Larry Shaw . " You 're Gonna Love Tomorrow " , as well as all subsequent episodes , takes place five years after the events of the fourth season finale following Cherry 's decision to revamp the series with a time jump .


Arora D. ( 1986 ) . Mushrooms Demystified : A Comprehensive Guide to the Fleshy Fungi . Berkeley , California : Ten Speed Press . ISBN 0 @-@ 89815 @-@ 169 @-@ 4 .


At the time of her birth , it was unknown if she was a male or female , as it was considered too dangerous to approach her and her parents . Her handlers assumed she was a male and originally named her " Sonny Jim " . When it was established that Pattycake was a female , a contest was held by the New York Daily News to find her a name . " Patty Cake " , the winning entry , was submitted by New York fireman John O 'Connor , who named the gorilla after his wife and a proposed daughter . " It just so happens that we have three boys and I told my wife that if we ever have a girl , we should name her Patty , which happens to be her name . I thought we 'd let the baby gorilla use the name in the meantime , " O 'Connor told reporters .


Innis got his first taste of university teaching at Chicago , where he delivered several introductory economics courses . One of his students was Mary Quayle , the woman he would marry in May 1921 when he was 26 and she 22 . Together they had four children , Donald ( 1924 ) , Mary ( 1927 ) , Hugh ( 1930 ) and Ann ( 1933 ) . Mary Quayle Innis was herself a notable economist and writer . Her book , An Economic History of Canada , was published in 1935 . Her novel , Stand on a Rainbow appeared in 1943 . Her other books include Mrs. Simcoe 's Diary ( 1965 ) , The Clear Spirit : Canadian Women and Their Times ( 1966 ) and Unfold the Years ( 1949 ) , a history of the Young Women 's Christian Association . She also edited Harold Innis 's posthumous Essays in Canadian Economic History ( 1956 ) and a 1972 reissue of his Empire and Communications .


Pattycake moved permanently to the Bronx Zoo on December 20 , 1982 . For a few years , she lived in a cage with Pansy , a chimpanzee . In June 1999 , Pattycake moved into the Wildlife Conservation Society 's $ 43 million Congo Gorilla Forest exhibit . The exhibit includes a Great Gorilla Forest viewing area that separates gorillas and visitors with a glass window . Two troops of gorillas inhabited the 6 @.@ 5 acre exhibit , with a dozen gorillas in Pattycake 's troop alone , including Fubo , Pattycake , Tunko , Triska , Halima , Fran , Layla , Kumi , Suki , Babatunde , Barbara , and M 'domo . The general curator of the Bronx Zoo , James Doherty , described Pattycake as " independent " with " few close friends " in the Congo Gorilla Forest . " It may have something to do with the fact that she didn 't live with her parents that long , and lived with that chimpanzee for a few years , " Doherty said .


The episode was directed by Mark Kirkland , and written by David Sacks . It features numerous guest stars , including Anne Bancroft as Dr. Zweig . Additionally , Ted Danson , Woody Harrelson , Rhea Perlman , John Ratzenberger , and George Wendt appear as their characters from Cheers . It received positive reception from television critics , and acquired a Nielsen rating of 9 @.@ 6 . The authors of I Can 't Believe It 's a Bigger and Better Updated Unofficial Simpsons Guide commented positively on the episode , as did reviews from DVD Verdict and DVD Movie Guide .


As Homer is looking for a place to drink , he tries a lesbian bar , the She She Lounge . However , he soon realizes that " this lesbian bar doesn 't have a fire exit " . He then leaves , saying " Enjoy your deathtrap , ladies ! " . This is a reference to a famous Greenwich Village gay bar , the Stonewall Inn . This mafia @-@ owned and -run bar " had no rear exit , so if there had been a fire on a weekend night , hundreds of customers would have had to escape through a single narrow passage leading to the front door . " The films Homer rents are Hero , Fearless , and Alive , the latter of which Marge watches . All of them involve plane crashes .


Given the strong resistance exhibited by the Chinese , the Canadians expected a tough fight as 25th Brigade prepared to assault its objectives as part of the second phase of the divisional plan . Yet with the loss of Hill 355 and 210 the Chinese unexpectedly withdrew from their well @-@ prepared defensive positions , with Hill 159 and 175 captured without opposition . Only the 2nd Battalion , Princess Patricia 's Canadian Light Infantry encountered any opposition before they captured the two Hill 187 features , losing one killed and six wounded during stiff fighting in which 28 Chinese were also killed . Indeed , the ease with which the Canadians had captured their initial objectives allowed them to press on , attaining their final objectives on the Jamestown Line by nightfall . No further resistance was encountered , although heavy Chinese artillery fire caused a number of casualties , including three killed . The Canadians subsequently occupied the positions they were destined to hold for the next twenty @-@ two months of fighting .


In its original American broadcast , " Fear of Flying " finished 48th ( tied with Dateline NBC ) in the ratings for the week of December 12 to December 18 , 1994 , with a Nielsen rating of 9 @.@ 6 . The episode was the third highest rated show on the Fox network that week , beaten only by Beverly Hills , 90210 , and Married ... with Children . Since airing , the episode has received many positive reviews from fans and television critics . In July 2007 , Simon Crerar of The Times listed the Cheers cast 's performance as one of the thirty @-@ three funniest cameos in the history of the show .


Cadmium is a chemical element with symbol Cd and atomic number 48 . This soft , bluish @-@ white metal is chemically similar to the two other stable metals in group 12 , zinc and mercury . Like zinc , it demonstrates oxidation state + 2 in most of its compounds , and like mercury , it has a lower melting point than other transition metals . Cadmium and its congeners are not always considered transition metals , in that they do not have partly filled d or f electron shells in the elemental or common oxidation states . The average concentration of cadmium in Earth 's crust is between 0 @.@ 1 and 0 @.@ 5 parts per million ( ppm ) . It was discovered in 1817 simultaneously by Stromeyer and Hermann , both in Germany , as an impurity in zinc carbonate .


Pattycake , also known as Patty Cake ( September 3 , 1972 – March 31 , 2013 ) was a female western lowland gorilla ( Gorilla gorilla gorilla ) born to Lulu and Kongo at the Central Park Zoo in New York City . She was the first baby gorilla successfully born in captivity in New York . Months after her much publicized birth , Pattycake 's arm was broken when it got stuck in her cage as her mother grabbed her away from her father . The incident was sensationally anthropomorphized in the media as a domestic dispute between Lulu and Kongo , but in reality experts thought it was a simple accident .


The club won their first FA Cup in 1887 with captain Archie Hunter becoming one of the game 's first household names . Aston Villa were one of the dozen teams that competed in the inaugural Football League in 1888 with one of the club 's directors , William McGregor being the league 's founder . Aston Villa emerged as the most successful English club of the Victorian era , winning no fewer than five League titles and three FA Cups by the end of Queen Victoria 's reign . In 1897 , the year Villa won The Double , they moved into their present home , the Aston Lower Grounds . Supporters coined the name " Villa Park " ; no official declaration listed the ground as Villa Park .


Complementary to the field of structural genomics , protein structure prediction seeks to develop efficient ways to provide plausible models for proteins whose structures have not yet been determined experimentally . The most successful type of structure prediction , known as homology modeling , relies on the existence of a " template " structure with sequence similarity to the protein being modeled ; structural genomics ' goal is to provide sufficient representation in solved structures to model most of those that remain . Although producing accurate models remains a challenge when only distantly related template structures are available , it has been suggested that sequence alignment is the bottleneck in this process , as quite accurate models can be produced if a " perfect " sequence alignment is known . Many structure prediction methods have served to inform the emerging field of protein engineering , in which novel protein folds have already been designed . A more complex computational problem is the prediction of intermolecular interactions , such as in molecular docking and protein – protein interaction prediction .


Short proteins can also be synthesized chemically by a family of methods known as peptide synthesis , which rely on organic synthesis techniques such as chemical ligation to produce peptides in high yield . Chemical synthesis allows for the introduction of non @-@ natural amino acids into polypeptide chains , such as attachment of fluorescent probes to amino acid side chains . These methods are useful in laboratory biochemistry and cell biology , though generally not for commercial applications . Chemical synthesis is inefficient for polypeptides longer than about 300 amino acids , and the synthesized proteins may not readily assume their native tertiary structure . Most chemical synthesis methods proceed from C @-@ terminus to N @-@ terminus , opposite the biological reaction .


A new badge was revealed in May 2007 , for the 2007 – 08 season and beyond . The new badge includes a star to represent the European Cup win in 1982 , and has a light blue background behind Villa 's ' lion rampant ' . The traditional motto " Prepared " remains in the badge , and the name Aston Villa has been shortened to AVFC , FC having been omitted from the previous badge . The lion is now unified as opposed to fragmented lions of the past . Randy Lerner petitioned fans to help with the design of the new badge .


Many proteins are involved in the process of cell signaling and signal transduction . Some proteins , such as insulin , are extracellular proteins that transmit a signal from the cell in which they were synthesized to other cells in distant tissues . Others are membrane proteins that act as receptors whose main function is to bind a signaling molecule and induce a biochemical response in the cell . Many receptors have a binding site exposed on the cell surface and an effector domain within the cell , which may have enzymatic activity or may undergo a conformational change detected by other proteins within the cell .