[20], and strongly stained Gleason pattern 5 cells can be seen in B for banked tumor specimens 01-009E and 02-034A.']",10.1371/journal.pone.0045656.g006,yes
PMC8665564,Figure_3,oa_package/c8/a5/PMC8665564.tar.gz,"[' 3A C).', ' 3D, E).', ' 3F H).', 'The impacts of sTREM2 fragments on amyloid deposition in 5xFAD mice.', 'H Quantitation of the amyloid plaque deposition in F (n = 8 mice, 24 fields of each group for analysis, paired Student s t test)One important feature of the full-length sTREM2 protein is that it enhances the clustering of microglia to the vicinity of the plaque [16].']","Fig. 3 The impacts of sTREM2 fragments on amyloid deposition in 5xFAD mice. and The 7-month-old 5xFAD mice were injected with the Fc-tagged sTREM2 fragment 4181 or Fc alone into the right and left hippocampi, respectively. Coronal sections were stained with DAPI (blue) for nuclei, MOAB-2 (green) for A, and Iba1 (red) for microglia. Coronal sections were stained with Thio-S (blue) for dense-core plaque, and Iba1 (red) for microglia. Representative z-stack images of the hippocampus regions are shown. Original magnification20; scale bar, 100m. Quantitation of the Iba1-positive area in A ( =8 mice, 24 fields of each group for analysis, paired Students test). Quantitation of the amyloid plaque deposition in A ( =8 mice, 24 fields of each group for analysis, paired Students test). Quantitation of the Thio-S-positive area in D ( =5 mice, 15 fields of each group for analysis, paired Students test). The 7-month-old 5xFAD mice were injected with the Fc-tagged sTREM2 fragment 5181 or Fc alone into the right and left hippocampi, respectively. Coronal sections were stained with DAPI (blue) for nuclei, MOAB-2 (green) for A, and Iba1 (red) for microglia. Representative z-stack images of the hippocampus regions are shown. Original magnification20; scale bar, 100m. Quantitation of the Iba1-positive area in F ( =8 mice, 24 fields of each group for analysis, paired Students test). Quantitation of the amyloid plaque deposition in F ( =8 mice, 24 fields of each group for analysis, paired Students test)",yes
PMC7118982,Figure_7,oa_package/ea/de/PMC7118982.tar.gz,"['Infection of 22-day-old C57Bl/6 mice with reference and E protein mutants revealed that the infectious titer in the brain (\nA) and the spinal cord (', 'However, the PBM mutant RNA was detected in the brain at 5 and 9 dpi, although at a lower level than the reference virus or the TMD mutant (C), indicating that this virus was capable of replicating at a low level in the brain.', 'Sequencing of the E and M genes again revealed the absence of reversion, again suggesting that the absence of detectable infectious virion production previously observed () was probably due to the low infectious dose.', 'The E protein TM and PBM domain are essential for optimal replication in the murine brain and spinal cord.', '2.']","Fig. 7 The E protein TM and PBM domain are essential for optimal replication in the murine brain and spinal cord. Infectious viral particles were quantified in (A) the brains and (B) the spinal cord of 22-day-old C57Bl/6 mice infected by the IC route with 10 TCID /10L rOC/ATCC, rOC/E-TM-Q17A or rOC/E-PBM-8284 over a period of 15 days. (C) Viral RNA was detected and quantified by RT-qPCR in the brain of infected mice at 5 and 9 days post-infection. LOD, limit of detection. Representative of three different experiments.",yes
PMC4011745,Figure_2,oa_package/93/a4/PMC4011745.tar.gz,"['g001""/>Specimen preparationAfter the micro-CT scan, the fresh femoral head specimens were processed ().', 'g002Cutting method of the femoral heads.', 'g002""/>Histological analyse of non-decalcified bone tissuePart c of the necrotic femoral head sample (. 2C) was fixed with 4% paraformaldehyde, dehydrated conventionally, cleared with xylene, and embedded in poly(methyl methacrylate) (PMMA) (Sigma-Aldrich, St.', 'Histological analyse of decalcified bone tissuePart a was subdivided into subchondral bone, necrotic, sclerotic, and healthy regions (. 2C).']",10.1371/journal.pone.0096361.g002,yes
PMC7750631,Figure_5,oa_package/14/cc/PMC7750631.tar.gz,['Observation of dendritic dystrophy in hippocampal formation in primary age-related tauopathy (PART) case in sortilin immunohistochemistry.'],"Figure 5 Observation of dendritic dystrophy in hippocampal formation in primary age-related tauopathy (PART) case in sortilin immunohistochemistry. Sortilin labeling in the section adjacent to the one shown as (case #22 in ). Labeling in the comparable region in a case without cerebral pTau or A pathologies (case #32). The framed areas are enlarged as indicated. In the PART case , fusiform swellings (as pointed by arrowheads) are seen on the immunolabeled dendritic processes in the s.r. In contrast, no swelling is found on the dendritic processes in the control case . Other abbreviations are as defined in . Scale bars are as indicated.",yes
PMC8582972,Figure_2,oa_package/3f/92/PMC8582972.tar.gz,"['suis in the corpus and antrum of the stomach was confirmed after sampling, in both the short- and long-term infected mice (A,B).', 'suis DNA was detected in the stomach of control mice intragastrically inoculated with sterile culture medium (A,B).', 'suis-infected mice (Supplementary S1), indicating gastric inflammation as scored by the Updated Sydney System (C,D) was more pronounced in long-term compared to short-term infection.', 'suis-infected animals also displayed increased expression of pro-inflammatory cytokines Il1 and Kc in the corpus of the stomach, which was significant in case of long-term infection (E H), while no changes in expression levels of other inflammatory markers (i.', 'Both short- and long-term gastric Helicobacter suis (H.']","Figure 2 Both short- and long-term gastric ( ) infection is associated with gastric colonization and inflammation. ( , ) colonization in the stomach (corpus and antrum) of mice infected with or the control broth for a short- (n = 911) ( ) and long-term period (n = 10) ( ), 7 days after instrastriatal injection with either 6-OHDA (black) or vehicle (white). ( , ) Gastric inflammation score according to the Updated Sydney System based on haematoxylin and eosin (H&E) staining of the stomach of control and -infected mice for short- (n = 911) ( ) and long-term infection (n = 10) ( ). ( ) Relative mRNA gene expression of the cytokine interleukin 1 ( ) and the chemokine keratinocyte chemoattractant ( ) in the corpus of the stomach after short- (n = 911) ( ) and long-term infection (n= 710) ( ). Data were analyzed by the MannWhitney test. ** 0.001 < 0.01; *** 0.0001 < 0.001; **** < 0.0001.",yes
PMC8874917,Figure_1,oa_package/20/d2/PMC8874917.tar.gz,"['The thoracic wall defect from the fifth to the eighth rib measured 30 cm 15 cm (A).', 'This area was covered with 2-mm Gore-Tex Dual Mesh, and size 1 nylon was used to suture the remaining thoracic wall edges (B).', 'The deviated and unstable fourth and ninth ribs were fixed with 2-mm titanium mandibular locking plates (Synthes MLP; Synthes Maxillofacial, Paoli, PA) (C).', 'A) The crushed fifth to eighth ribs were removed, and the intercostal artery vascular sheath was ligated at the same level to achieve hemostasis.']","Figure 1 A) The crushed fifth to eighth ribs were removed, and the intercostal artery vascular sheath was ligated at the same level to achieve hemostasis. The large anterolateral chest wall defect measured 30cm15cm. B) Gore-Tex Dual Mesh was sutured with an approximately 1-cm margin from the edge, pulling the mesh with adequate tension, and trimming step-by-step with fixation. C) The thoracic wall was reconstructed using Gore-Tex Dual Mesh and titanium plates. The inlay of the Dual Mesh was sutured to the thorax, and titanium plates were used for fixation of the deviated fourth and ninth ribs.",yes
PMC11250286,Figure_2,oa_package/6f/62/PMC11250286.tar.gz,"['All measurements of lesions in this study were performed in the axial plane, and any disagreement in describing semantic features was resolved by a consensus read ().', 'Cases of image evaluation.']","Figure 2 Cases of image evaluation. (A) Image from a 58-year-old male patient with lung adenocarcinoma, with the pathological results indicating no lymph node metastasis. The axial CT scans show an irregular part-solid nodule (long diameter of 19 mm, short diameter of 15 mm) located in the left upper lung. The nodule has superficial lobulation and spiculation at the margin and is pulling the pleura without indentation. A total of 20 negative lymph nodes were detected during the operation, including 2 at stations 24, 4 at stations 56, 1 at stations 79, and 13 at stations 1014. (B) Image from a 67-year-old male patient with lung adenocarcinoma, with pathological results indicating lymph node metastasis. The axial CT scans show an irregular part-solid nodule (long diameter of 42 mm, short diameter of 34 mm) located in the left upper lung directly in contact with the interlobar fissure pleura; the patient also had emphysema. A total of 30 lymph nodes were detected during the operation (7 at stations 24, 5 at stations 79, and 18 at stations 1014), among which 1 cancerous lymph node was located at stations 24. CT, computed tomography.",yes
PMC5990258,Figure_3,oa_package/63/b4/PMC5990258.tar.gz,['Segment of the left popliteal artery with multiple cysts containing mucin in the adventitial layer.'],Figura 3 Segmento da artria popltea esquerda com mltiplos cistos de contedo mucinoso na camada adventcia.,yes
PMC10474302,Figure_10,oa_package/80/71/PMC10474302.tar.gz,[],"Figure 4. Alternative oxidase (AOX) expression on a mutator background leads to activation of the mitochondrial integrated stress response (ISRmt) and inflammatory pathways. Transcripts with the highest experimental fold change differing significantly in AOXMut versus Mut in RNA sequencing (RNA-seq) data of mouse skeletal muscle shown as a volcano plot. Top 10 activated upstream regulators in AOXMut compared with Mut in RNA-seq data of mouse skeletal muscle analyzed using Ingenuity Pathway Analysis (IPA). Comparison of the most significant canonical pathways changed in AOXMut versus Mut based on RNA-seq data of mouse skeletal muscle analyzed using IPA. Heatmaps of RNA-seq data of mouse skeletal muscle analyzed using IPA showing (D) mitochondrial integrated stress response genes and (E) acute phase response genes. WT (n = 5), AOX (n = 5), mutator (n = 5), and AOXmutator (n = 3), biological replicates, RNA-seq for each sample was performed once. Abbreviations: WT, wildtype mice; AOX, AOX mice; AOXMut, AOXmutator mice; Mut, mutator mice.",yes
PMC7702191,Figure_6,oa_package/4b/d5/PMC7702191.tar.gz,[],"Figure5 Cytoskeleton Dynamics during RCD Analyzed by Actin and Alpha-Tubulin Immunofluorescence (A) Structure of control L929sAhFAS cells, the DNA content is stained with Hoechst 33342 (blue), actin is stained with Rhodamine Phalloidin (red), and Alpha-Tubulin is stained with Alexa Fluor 488 antibody (green) (scale bar, 10m). (BE) Series of four panels for each RCD modality showing the changes in the cytoskeleton during the RCD process. Left to right: a schematic of the measured mechanical changes, elasticity changes indicated by a dotted line and triangles, microrheological changes indicated by full line and circles. Black arrows pointing to dashed lines: time points at which the state of the cytoskeleton was analyzed, followed by confocal images at these respective time points (scale bar, 10m). (B) Intrinsic apoptosis: cellular shrinkage with typical long remaining focal adhesion points containing irregularities (white arrows), fast cytoskeleton degradation. (C) Intrinsic apoptosis: cellular shrinkage followed by blebbing (white arrows), a short period of concentration of cytoskeleton near the nucleus is followed by rapid degradation. (D) Necroptosis is characterized by overall rounding of the cell; cytoskeletal structure remains intact during earlier time points. (E) In ferroptosis, the cytoskeleton remains intact during earlier time points, membrane blebs can be distinguished (white arrows).",yes
PMC9093211,Figure_3,oa_package/f8/1b/PMC9093211.tar.gz,['33-year-old female patient with retroperitoneal abscess due to injury at the level of the second continent of the duodenum after nephrectomy.'],Figure 3 33-year-old female patient with retroperitoneal abscess due to injury at the level of the second continent of the duodenum after nephrectomy. ) Leakage of oral contrast agent from the damaged duodenum to retroperitoneal space. ) Pararenally located abscess. ) Computed tomography-guided insertion of entry needle into the abscess cavity and insertion of 8 F drainage catheter. ) The abscess was fully regressed 12 days later and the drainage catheter was removed,yes
PMC11365134,Figure_5,oa_package/f4/4f/PMC11365134.tar.gz,"[' 5A).', ' 5B).', ' 5B).', ' 5C).', ' 5D).', '\nHK2 regulates lactylation of H3K18.', '01\nH3K18 lactylation in promoter increases HK2 expressionA previous study has indicated that H3K18 lactylation is a label for active promoters and enhancers [28].']","Fig. 5 HK2 regulates lactylation of H3K18. ( ) Western blotting was conducted to measure pan histone lactylation (pan-kla) and H3K18 lactylation (H3K18la) levels in WT and HK2-KO mice in the sham and I/R groups. H3 and GAPDH were the internal control of H3K18la and pan-kla, respectively. ( ) Pan-kla and H3K18la levels were detected using western blotting in HK-2 cells treated with H/R and transfected with sh-HK2. ( ) After HK2 knockdown and LA treatment, the levels of HK2 and H3K18la were measured using western blotting. ( ) After HK2 overexpression and 2-DG treatment, the levels of HK2 and H3K18la were examined using western blotting. ** <0.01",yes
PMC3793586,Figure_2,oa_package/4b/a0/PMC3793586.tar.gz,"['Microscopically, the tumor was composed of round or polygonal cells arranged in characteristic compact cell nests (Zellballen) or trabecular patterns bound by a delicate fibrovascularstroma ().', '001""/> (HEx200) Tumor cells arranged in nests and trabecular patterns surrounded by an endocrine stroma.']",Figure 2 (HEx200) Tumor cells arranged in nests and trabecular patterns surrounded by an endocrine stroma.,yes
PMC4519563,Figure_3,oa_package/0a/a9/PMC4519563.tar.gz,"['MRI on the left hip () also showed complete bone reabsorption of the proximal femur, along with the presence of a vascular mass in the joint space and invasion of the fossa and the upper wall of the left acetabulum and wing of the ipsilateral ilium.', 'MRI of the pelvis (coronal slice).']",Fig. 3 MRI of the pelvis (coronal slice).,yes
PMC4518598,Figure_2,oa_package/14/0c/PMC4518598.tar.gz,"[' 2).', 'Summary of 34 nodules from 33 patients using concomitant percutaneous needle aspiration and localization protocolDiscussionImproved imaging techniques such as high resolution, single breath, or spiral CT scanning, result in more frequent identification of small and often sub-centimetric SPNs [4].']",Fig. 2 Summary of 34 nodules from 33 patients using concomitant percutaneous needle aspiration and localization protocol,yes
PMC11031648,Figure_2,oa_package/c2/9c/PMC11031648.tar.gz,"['CT, computed tomographyCT scan of the abdomen and pelvis with intravenous contrast showing an appendicular mass.']","Figure 2 CT scan of the abdomen and pelvis with intravenous contrast showing an appendicular mass. CT, computed tomography",yes
PMC3691094,Figure_2,oa_package/02/59/PMC3691094.tar.gz,"['The size of the lesion was 12 cm (cranio-caudal) 3,5 cm (sagittal) 4 cm (transversal) (A).', 'Whole body bone scan showed the increased uptake of the tracer in the diaphysis of the left femur, but no evidence of further lesions (B).']","FIGURE 2A T1 weighed MR imaging detects a hyperintense, contrast medium enhanced, lesion in the mid of the left femur. Cortical destruction can be seen. The biopsy tract can be seen on the lateral side.",yes
PMC6921582,Figure_8,oa_package/2b/b9/PMC6921582.tar.gz,"[' 8) showed neuroendocrine tumors (G2, mitotic figures: 1/10 HPF).', 'a HE staining; b CgA (+); c Syn (+)Discussion and conclusionsInsulinoma is the most common functional pancreatic neuroendocrine tumor derived from islet -cells.']",Fig. 8 Case 2: Postoperative pathology confirmed a diagnosis of neuroendocrine tumor. HE staining; CgA (+); Syn (+),yes
PMC6020095,Figure_3,oa_package/bd/16/PMC6020095.tar.gz,"['An abdominal X-ray demonstrated a paucity of bowel gas centrally with a few loops of small bowel appearing mildly dilated with a single air-fluid level, possibly indicating an early intestinal obstruction ().', 'Given his history of Peutz-Jeghers polyps causing intussusception requiring operative intervention, the patient was urgently taken to the operating room for a diagnostic laparoscopy, reduction of the presumed intussusception, and possible further bowel resection.']","Fig. 3 Abdominal X-rays before the second surgery. There is a paucity of bowel gas centrally with a few loops of small bowel appearing mildly dilated with a single air-fluid level, possibly indicating an early intestinal obstruction. Note the very small titanium staples in the upper left quadrant of the abdomen. (A) Supine. (B) Left decubitus.",yes
PMC4540492,Figure_4,oa_package/2f/42/PMC4540492.tar.gz,"['ref044"" ref-type=""bibr"">44], we observed no difference in the lesion size or pathology between wild-type and Il22\n-/- mice when infected with 2 x 103 parasites (A).', 'On the other hand, Il22\n-/- mice infected with 2 x 106 and 2 x 107 parasites had more pathology than their wild-type counterparts (B and 4C).', 'We then measured levels of IL-22 expression in the lesions, and found significantly higher expression of Il22 mRNA when mice were infected with more parasites (D).', 'Unlike IL-22, IL-22BP was expressed at significantly lower levels when mice were infected with more parasites (E).', 'g004The requirement for IL-22 is parasite dose dependent.']",10.1371/journal.pone.0134698.g004,yes
PMC10711094,Figure_1,oa_package/06/cc/PMC10711094.tar.gz,[],"Figure1 shows the preoperative image of the patient: CT of the brain revealed an irregular jumble density mass with a well-defined boundary of the right thalamus. The right lateral and third ventricles were compressed and deformed, and the center line structure shifted to the left. MRI results of the brain revealed irregular mixed signals and a remarkably enhancing mass of the right thalamus with multiple cystic lesions and a liquidliquid plane. shows the image of the patient 12 years after the operation: MRI results of the brain revealed some abnormal signals of the medial area of the right temporal lobe, right cerebral foot, cisterna annulus, CPA area, and pontine arm. MRI results of the brain revealed a new abnormal enhancing mass of the spinal canal (C1-C2 level). MRI results of the cervical vertebra revealed multiple spinal cord spread.",yes
PMC10349115,Figure_2,oa_package/e3/92/PMC10349115.tar.gz,"[' 2a), both in focal area of parenchyma (arrows) and in vessels (asterisks).', ' 2a).', ' 2a).', ' 2a).', 'S1 injection induces endothelial C3 deposits and increases C3aR expression in lung tissue of hACE2-KI mice.', 'Since the activation of complement system and exuberant C3 deposits lead to increased C3a generation25,26, we further investigated the possible detrimental activity of C3a/C3a receptor (C3aR) axis in S1-induced lung tissues injury.', ' 2b), while it mainly localized in bronchiolar epithelial cells (Supplementary 2a, asterisks).', ' 2b).']","Figure 2 S1 injection induces endothelial C3 deposits and increases C3aR expression in lung tissue of hACE2-KI mice. ( ) Representative images of C3 deposits (green) in lung vessels (asterisk) positive for vWF (red) and in lung parenchyma (arrows) in CTR (n=4) or S1-injected mice at 3 (n=3) and 7d (n=6). Insets display vascular C3 deposition. Endothelial C3 deposits are quantified and expressed as % of C3 positive vessels. Lung structures and nuclei are counterstained with wheat germ agglutinin (WGA) lectin (white) and DAPI (blue), respectively. Scale bars: 20m. Results are presented as meanSEM. * <0.05 and ** <0.01 versus CTR. ( ) Representative images of C3aR staining (red) in lung vessels and in lung parenchyma in CTR (n=4), S1-injected mice at 3d (n=3), and 7d (n=6). Lung structures and nuclei are counterstained with WGA lectin (green) and DAPI (blu), respectively. Scale bars: 20m.",yes
PMC6034844,Figure_1,oa_package/41/06/PMC6034844.tar.gz,"['4 covered similar portions of the gray matter in non-demented controls compared to patients with early ADD ().', 'g001Representative amyloid-beta (A ) immunohistochemistry demonstrates similar levels of pathology between elderly high-pathology controls and elderly subjects with mild ADD.']",10.1371/journal.pone.0200251.g001,yes
PMC3795520,Figure_3,oa_package/ea/99/PMC3795520.tar.gz,['Parietal bone with hemangioma.'],Figure 3 Parietal bone with hemangioma.,yes
PMC6054664,Figure_1,oa_package/53/8e/PMC6054664.tar.gz,"[' 1 shows images of matching levels from the two scanners.', ' 1C illustrates that the image resolution is visibly higher with the Tomopath scanner and allowed differentiation of the layers within the vessel wall.', 'Comparing a prototype tomographic scanner dedicated to histopathology (Tomopath) with a commercial tabletop micro-CT scanner.', 'Correlative detection of two forms of calcificationCareful evaluation of the tomographic and histology images at the same sectioning levels revealed two forms of micro calcifications.']","Figure 1 Comparing a prototype tomographic scanner dedicated to histopathology (Tomopath) with a commercial tabletop micro-CT scanner. ( ) Cross-sectional image at 1.3mm level below the surface of a paraffin block from the Tomopath scanner. Left-anterior-descending (LAD) coronary artery segments of a deceased HIV patient are embedded in the block. The vessel walls appear bright against a dark background from the x-ray absorption contrast between tissue and paraffin medium. The grid-like shadows are cast by the plastic grid of the embedding cassette. ( ) Cross-sectional image at the same level from the commercial tabletop micro-CT scanner. ( ) Zoomed-in view of the top left LAD segment outlined in A from the Tomopath scanner. The layers of the vessel wall are visible. The scan times of the micro-CT and the Tomopath scanners were 2.75hours and 15minutes, respectively. ( ) Picture of the intact paraffin block prior to histological sectioning.",yes
PMC7842189,Figure_6,oa_package/e5/57/PMC7842189.tar.gz,"['We performed single nuclear sequencing of 107,620 nuclei from pre-central gyrus from patients having behavioral variant FTD (bvFTD) with tau immunoreactive inclusions (Pick s disease) and matched controls, identifying 2,891 microglial nuclei for this analysis after quality control (QC), clustering and cluster annotation (A).', 'Gene Ontology (GO) and PPI analyses demonstrate additional pathway-level alignment between M_UP3 and bvFTD microglia, including antigen presentation, IFN- signaling, and regulation of cell death (C), further confirming that the latent microglia disease states that we identified in mice are relevant to human disease.', 'In addition, to account for the random effect of subject in our analysis, we calculated differential expressed genes using a mixed effects model with subject as a random effect, and confirmed a very high Pearson s correlation between the diagnosis effects (beta) calculated from the linear model and mixed effects models (B; Pearson s correlation = 0.', '.']","Figure 6. Microglia from Patients with FTD Upregulate the Later-Stage Immune Pathways Identified in Mice with Tau Pathology (A) Seurat object of nuclei sequenced from bvFTD patients with tau pathology (Picks disease, precentral gyrus, n = 7) and control (no pathology, precentral gyrus, n = 8), showing the cell cluster enriched for microglial-specific marker genes (green, top) and cells from bvFTD (pink) and control (blue) patients. (B) Scatterplot and Pearsons correlation of effect size () of differential gene expression between bvFTD versus control microglia, comparing results from a linear model (x axis) and mixed-effects model with subject as a random effect (y axis) (n = 7,989 genes). (C) PPI plot of genes significantly upregulated in bvFTD microglia compared to controls (log2fc 0.1, FDR < 5E4), highlighting genes with PPI in significant GO pathways ( > 2). The asterisk indicates functional overlap with M_UP3. (D) Bar plot of genes that are differentially expressed in bvFTD versus control microglia (log2fc scale) and either activate IFN- (blue) or mediate IFN- signaling (red), (linear model; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.001). See also and .",yes
PMC2972610,Figure_4,oa_package/4e/f0/PMC2972610.tar.gz,"['While the R II data were determined to be in the more important category for the thoracic cage (at the 3 points level), more data were provided compared to R I (unimportant category weighted) with a 2 point average for the pelvic cage ().', 'A case of thoracic cage injury (out of vehicle accident).']","Figure 4 A case of thoracic cage injury (outofvehicle accident). ) 3D Endothoracic view image shows multiple rib fractures (arrows), ) although these are barely visible with external view 3D image (arrow). , ) outlet view superiorly and frontview images are excellent to evaluate bony and osseous structure together. A left cervical rib variant is visible in two images (black arrow in c, white arrow in d). ) Coronal oblique 3D view shows the defect at the left medial diaphragmatic contours (dashed black line and arrow). ) The defect area is also visible with the axial conventional image (dashed ellipse and arrow).",yes
PMC9366061,Figure_2,oa_package/78/f4/PMC9366061.tar.gz,[],"Figure2 Real-time image of intraoperative ectopic adrenal tumor. The tumor was located behind the anterior lip of the left kidney, and the lower edge of the tumor was adjacent to the renal vein.",yes
PMC10679543,Figure_2,oa_package/74/6b/PMC10679543.tar.gz,"['3%) were judged as definite AKAs ().', ', the dominant anterior radiculomedullary artery), this additional vessel was considered as another anterior radiculomedullary artery by the consensus of the two interventional radiologists ().', '(a-d) Intraprocedural images of a 66-year-old female patient.']","Figure 2 (a-d) Intraprocedural images of a 66-year-old female patient. Selective angiography (a) of the left eighth intercostal artery (ICA) (thick arrow) demonstrated the ICA branching off an obscured hairpin-curved vessel (thin arrow) that ran towards the midline in the arterially enhanced phase. This obscured hairpin-curved vessel was barely recognizable with very careful observation and was determined by two interventional radiologists to be a possible artery of Adamkiewicz (AKA). The sign of systemictopulmonary artery shunting (dotted circle) indicated the ICA was involved in bleeding. The corresponding contrast-enhanced oblique coronal cone-beam computed tomography (CT) images (b, c) of the left ICA clearly demonstrated the hairpin-curved AKA (arrow in b, c) connecting to the anterior spinal artery (ASA) (dotted arrow in b, c) and the presence of an additional anterior radiculomedullary artery (thick arrow in c) that originated from the dorsal branch of the left ninth ICA (curved arrow in c) because of the inflow of contrast medium through the anastomosis, which was not detected on angiography. The corresponding contrast-enhanced axial cone-beam CT image (d) of the left ICA demonstrated the ASA, which was considered as a dot enhancement (arrow) continuing cranio-caudally on the anterior spinal cord surface.",yes
PMC7509637,Figure_4,oa_package/70/b7/PMC7509637.tar.gz,['FapCS accelerated A induced cognitive and neuronal pathology in adult zebrafish.'],"Figure 3 FapCS accelerated A fibrillization and associated pathology in zebrafish larvae. A (100 10 ), FapCS (10 10 ) or A + FapCS at a 10:1 molar ratio (100:10 10 ) were injected to the cerebroventricular space of one week old zebrafish larvae. Congo red (100 10 ) was injected at 2 d or one week post A injection to stain and monitor the A fibrillization. A) The fluorescence images recorded via the brightfield and red fluorescence channel of a microscope for the wholemount dorsal and lateral side of larvae. B) Quantitative measurement of the relative red fluorescence intensity, indicative of Congoredstained A plaques, from the cerebral region of larvae ( = 10). A injected together with FapCS presented significantly stronger (**, < 0.005) fluorescence and thus elevated A fibrillization compared to A alone or buffer, at 2 d postinjection. FapCS did not show any retention of Congo red fluorescence in the brain. C,D) A induced behavioral pathology in terms of movement frequency (C) and total distance traveled by zebrafish larvae (D) ( = 10 per group and three groups per sample). The measurements were made for the observation period of 1 h at 2 d and one week postinjection. FapCS significantly aggravated (**, < 0.005) A toxicity and reduced the movement frequency and total travel distance of the larvae at 2 d postinjection. E) ROS generation in the brain homogenates of zebrafish larvae presented as relative DFC fluorescence. H O was used as positive control. Similar to behavioral toxicity, A + FapCS significantly enhanced ROS production in the larval brain ( = 10 per group and 3 groups per sample).",yes
PMC4941200,Figure_3,oa_package/16/ba/PMC4941200.tar.gz,"['It was clear the patient did, in fact, have malrotation with the cecum and appendix position being in the middle of the abdomen (a).', 'While Ladd s bands were clearly seen this was not the source of obstruction precipitating the patient s hospitalization as they did not course over the duodenum (b).', '']",Fig. 3 Intraoperative pictures demonstrate a midabdominal appendix. The appendix was perforated with a large fecolith (white arrow). The proximal jejunum (black arrow) is seen coursing the patients right side and not crossing midline along the normal anatomic course (3a). Ladds bands (white dotted lines) are seen originated from the cecum and right colon (3b).,yes
PMC5652376,Figure_1,oa_package/3e/bf/PMC5652376.tar.gz,"['In the majority of patients, ANCA antibody titers fell rapidly during the first 6 months post-biopsy ( 1), such that the median titers of both anti-MPO (18 IU/ml) and anti-PR3 (20 IU/ml) antibodies were within the normal range by 6 months post-biopsy.', ' 1(a) Anti-MPO and (b) anti-PR3 antibody titers at biopsy and at 6, 12, and 18 months post-biopsy in the ANCA+ve group.', 'One patient developed increasing anti-PR3 antibody titers during the first 6 months post-biopsy ( 1b).']","Figure1 Lines indicate paired data. Normal range indicated by the shaded area. Median anti-MPO and anti-PR3 antibody titers fell to within the normalrange at 6 months post-biopsy. ANCA, antineutrophil cytoplasmic antibody; ANCA+ve, antineutrophil cytoplasmic antibody positive; MPO, myeloperoxidase; PR3, proteinase3.",yes
PMC8826911,Figure_1,oa_package/f6/34/PMC8826911.tar.gz,"['The correct expression of NS2, NS2-Nt, or NS2-Ct by these rMVAs was evaluated and confirmed by immunofluorescence assay (IFA) (A).', '5) (B).', '5) immunization groups, which failed to induce a specific cellular immune response as expected (B).', '75) (C), which support the data of the in silico analysis that indicated the prevailing location of CD8+ T cell epitopes in the N-terminal half of the protein (Table 1).']","FIG 1 Cellular immune responses against BTV in mice immunized with MVA-NS2, MVA-NS2-Nt, and MVA-NS2-Ct. (A) Indirect immunofluorescence of DF-1 cells infected with MVA-NS2, MVA-NS2-Nt, MVA-NS2-Ct, or noninfected (control). NS2, NS2-Nt, and NS2-Ct (red) were detected using MAb 23H6 -NS2. Nuclei were stained with DAPI. Scale bars 20m. (B, C) Interferon gamma ELISPOT assay. Splenocytes of IFNAR(-/-) mice immunized with MVA-NS2, MVA-NS2-Nt, or MVA-NS2-Ct were stimulated with the recombinant proteins NS2-Nt (B) or NS2-Ct (C). Points represent individual values for each mouse, bars represent the mean values of each group and errors bars represent . Asterisks denote significant differences between stimulated splenocytes ( < 0.05) (The MannWhitney U test).",yes
PMC6406211,Figure_1,oa_package/23/1c/PMC6406211.tar.gz,['A 23 y old patient with congenital aortic hypoplasia: surgical aortic bypass demonstrated by computed tomography (panel A).'],Figure 1 A 23yold patient with congenital aortic hypoplasia: surgical aortic bypass demonstrated by computed tomography (panel A). A highgrade stenosis is seen of the right coronary artery (RCA) by computed tomographic coronary angiography (panel B) as well as invasive coronary angiography (panel C). Invasive coronary angiography of the RCA post stent placement (panel D),yes
PMC8700442,Figure_2,oa_package/c5/5e/PMC8700442.tar.gz,"['In recent studies, it was shown that DL image reconstruction is able to achieve an acquisition time reduction of up to 65% in prostate TSE imaging, without any loss of image quality () [11,38].', ' shows an example of a T2-weighted turbo spin-echo (TSE) image of the prostate in the axial plane, with standard reconstruction (SR) and deep learning reconstruction (DL) of a second, under-sampled acquisition of the same patient.', 'Similar to , two different acquisitions were performed (standard acquisition for SR and conventionally under-sampled acquisition for DL).']","Figure 2 shows an example of a T2-weighted turbo spin-echo (TSE) image of the prostate in the axial plane, with standard reconstruction (SR) and deep learning reconstruction (DL) of a second, under-sampled acquisition of the same patient. SR is shown on the left, with an acquisition time of 4:19 min. The acquisition time of DL was 1:20 min in the same patient (right-hand-side image). Motion artifacts were reduced due to the shortened acquisition time.",yes
PMC5161749,Figure_2,oa_package/28/de/PMC5161749.tar.gz,"['7 cells after fungal exposure ( 2A) and was paralleled by increased protein production, as revealed by immunofluorescence ( 2B) and immunoblotting ( 2C).', 'Similar results were obtained with purified lung macrophages (s 2D and 2E).', 'The production of IFN- was functional, as evidenced by the upregulated expression of IP10 gene ( 2F), and occurred in response to Dectin-1 and MyD88 signaling (s 2G and 2H) via NF-kB ( 2I).', 'Because DAPK1 expression paralleled that of IFN- (s 2J and 2K), these results suggest that IFN- production upon Dectin-1/MyD88 recognition of the fungus may autocrinally activate DAPK1 in phagocytic cells.', ' 2A.']","Figure1 Conidia Induce IFN--Dependent DAPK1 Expression InVitro and InVivo (AC) DAPK1 gene expression (A) and production by immunoblotting (B) and immunofluorescence (C) in RAW264.7 cells pulsed with live conidia in the presence of rIFN-. (D) DAPK1 expression in C57BL/6 lung macrophages after exposure to heat-inactivated resting conidia (RC), swollen conidia (SC), or hyphae. (E and F) DAPK1 gene (E) and protein (F) expression in lung of mice either uninfected (0 dpi, days post-infection) or infected with conidia and treated with rIFN-. (G) Immunoblotting of DAPK1 in purified lung macrophages exposed to live conidia invitro in the presence of rIFN-. gene expression was assessed by real-time qPCR and normalized on Gapdh. For immunoblotting, normalization was performed on mouse -actin (the corresponding pixel densities are indicated). For immunofluorescence, nuclei were counterstained with DAPI. Photographs were taken with a high-resolution microscope (Olympus BX51). Scale bars, 25m. DAPK1 fluorescence intensity was measured with the ImageJ software; BF, brightfield. Data (mean values SD) represent pooled results or representative images (immunofluorescence and immunoblotting) from three experiments. p< 0.05, p< 0.01, p< 0.001, p< 0.0001, rIFN- treated versus untreated RAW264.7 cells and rIFN- treated versus untreated mice. None, unpulsed and/or untreated cells. See also .",yes
PMC2928766,Figure_3,oa_package/5c/2d/PMC2928766.tar.gz,"['Score of leukocytes in kidney sections with inflammatory stage 1, 2, 3 and 4.']",Figure 3 . Mean +/- SEM of semi-quantitative score of leukocytes in kidney sections from slaughtered finishing pigs (a) and slaughtered sows (b) in a given group of inflammatory stage. Different letters (a-d) indicate significant differences in the mean score of a given kind of leukocyte between the four groups.,yes
PMC7427099,Figure_4,oa_package/5c/a8/PMC7427099.tar.gz,"[' 4A; Supplemental ', ' 4B).', 'Constitutive homozygous snx14 mutations do not impact zebrafish morphology at 4dpf but do increase FAs from neutral lipid and phospholipids.', 'Altered lipid profiles in snx14 mutant zebrafishIt has previously been reported that human SNX14 mutations disrupt neutral lipid metabolism.', ' 4C E).', ' 4F H).']","Figure 4 Constitutive homozygous mutations do not impact zebrafish morphology at 4dpf but do increase FAs from neutral lipid and phospholipids. ( ) Illustration and demonstration of zebrafish eye (E) width and head (H) width measurements of maternal zygotic (MZ) fish derived from female and male pairs. ( ) Maximum projected confocal images of heads (dorsal view) from 4dpf zebrafish embryos either or MZ- . Staining employed immunohistochemistry against acetylated tubulin (green), marking axon tracts and SV2 (magenta) marking neuropil areas. ( ) Relative FA levels from whole body lysates of 4dpf zebrafish. Neutral lipid fraction-derived FA 16:0 ( ), FA 18:1(n9) ( ) and FA 20:4(n6) ( ) were elevated in zebrafish compared to both and zebrafish. ( ) Phospholipid fraction-derived FA 16:0 ( ), FA 18:1(n9) ( ) and FA 20:4(n6) ( ) were elevated in both and zebrafish compared to zebrafish. =3 (Pool of 6 zebrafish in each lysate), circles=individual lysate values, bars=mean, error bars=SD, **( 0.01), n.s. ( 0.05), one-way ANOVA.",yes
PMC4976110,Figure_3,oa_package/91/77/PMC4976110.tar.gz,"['(A) Western blot images created by Compass Software (ProteinSimple, Santa Clara, CA, USA) representing modified and total histone expression in the prefrontal cortex (PFC) for each treatment group.']","Figure 3 Western blot images created by Compass Software (ProteinSimple, Santa Clara, CA, USA) representing modified and total histone expression in the prefrontal cortex (PFC) for each treatment group. No differences in total histone expression was observed for any histone protein. Levels of acetyl-H2b (AH2b), acetyl-H3 (AH3), and acetyl-H4 (AH4) were significantly decreased compared to sham. No differences were observed between injury groups. *Indicates < 0.05. Data expressed as mean SEM. ( = 8/group).",yes
PMC4956627,Figure_1,oa_package/87/8e/PMC4956627.tar.gz,"[' 1).', 'A 27-year-old woman with an enlarging palpable right breast mass admitted to the hospital for intractable joint pain.', 'c Photomicrograph (original magnification 200; haematoxylin-eosin [H-E] stain) showing non-necrotizing granulomatous inflammation (arrow) composed of histiocytes and giant cellsImaging demonstrates a varied appearance based on the timing of radiographic evaluation, extent of inflammation and possibility of prior intervention [4, 8, 11 15].', ' 1).', ' 1).', ' 1).', ' 1c).', ' 1c).', ' 1).', ' 1).']","Fig. 1 A 27-year-old woman with an enlarging palpable right breast mass admitted to the hospital for intractable joint pain. An erythematous rash was noted on her right breast and extremities. She was 8months post-partum, breastfeeding with difficulty on the right. Bilateral mediolateral oblique mammogram ( ) demonstrates regional asymmetry in the middle depth upper breast ( ). Clinical photograph of the patients forearm shows erythematous areas ( ), representing erythema nodosum. Photomicrograph (original magnification 200; haematoxylin-eosin [H-E] stain) showing non-necrotizing granulomatous inflammation ( ) composed of histiocytes and giant cells",yes
PMC7471426,Figure_4,oa_package/d1/e9/PMC7471426.tar.gz,[],"FIG. 4 CHS131 does not affect the fibrosis stage but reduces markers of fibrosis, stellate cell activation, and inflammation. Liver content in (A) hydroxyproline, (B) Col1a1, (C) SMA, and (D) Gal3. Images below (BD) represent IHC from NASH+vehicle and NASH+CHS131 high dose. For < 0.05 (onetailed ANOVA), * < 0.05, ** < 0.01, *** < 0.001 for posthoc LSD test for chow+vehicle (black stars) or NASHCHS131 low dose (red stars) or NASHCHS131 high dose (blue stars) compared to NASH+vehicle. Data represent meansSEMs.",yes
PMC10716465,Figure_4,oa_package/fd/6f/PMC10716465.tar.gz,[],"Figure4 Peritonitis affects specific peripheral DAMP levels in mice and PD patients. . C57BL/6J mice (n=5 per group) were injected intraperitoneally with live or PBS (Control) and blood was obtained at the indicated time points. C57BL/6 mice (n=6/group) were fitted with a peritoneal catheter, given a 7-day recovery period and instilled once daily with 2ml PBS or PDF for 14 days. Mice were then i.p. injected with or PBS (Day 0) and culled at Day 1 or further exposed daily to PBS or PDF, prior to culling at Day 28. Peritoneal lavages and blood were obtained at Day 1 and Day 28. Plasma and peritoneal levels of DAMPs were determined by ELISA. 0.05 0.01 005, ordinary one-way ANOVA (normal distribution) or Kruskal-Wallis test (non-normal distribution). Levels of the indicated DAMPs were determined by ELISA in plasma from the same patients (n=8) non-infected (n.i.) or at Day 1 of hospital admission due to peritonitis. Horizontal lines in indicate the median value for the group, symbols indicate individual data points. * 0.05; 0.01 (Peritonitis vs. non-infected, n.i.), Wilcoxon signed-rank test for paired samples.",yes
PMC11745293,Figure_9,oa_package/57/37/PMC11745293.tar.gz,"['28, 29 When seeded in a test tube, immobilized in a vertical position, the culture tends to run to the bottom of the tube (A).', '28PCC: (A) Cryptococcus spp.', 'III Species definition can be obtained from a sample of the original, primary culture, in Niger seed or Sabouraud dextrose medium, and then transplanted onto a medium containing canavanine, glycine, and bromothymol blue (CGB agar).', 'Species of the Cryptococcus neoformans complex do not produce such changes and the CGB agar remains with its original yellow-green color (B).']","Figure 9 PCC: (A) spp. Yeast culture; appearance of condensed milk. (B) and . CGB Agar. Yellowish-green tube, original CGB agar . Cobalt blue tube, modified CGB agar .",yes
PMC8304165,Figure_2,oa_package/54/b8/PMC8304165.tar.gz,"['Phase contrast microscopy revealed the presence of multinucleated elongated cells, containing from three to more than eight nuclei, related to myotubes (A).', 'After 9 days of myogenic induction, the multinucleated cells exhibited high MHC expression, as confirmed by immunofluorescence microscopy (B,C).', 'MHC protein expression was also evaluated by Western blot analysis at different time points (T0 3 6 9 days) of myogenic differentiation and revealed a gradual increase in MHC over time during myogenic differentiation progression (D), validating the suitability of the in vitro myogenesis model.', 'Myogenic differentiation: multinucleated cells (myotubes) observed after 9 days of myogenic induction, visualized by phase contrast microscopy, original magnification: 20 , scale bar 50 m (A).']","Figure 2 Myogenic differentiation: multinucleated cells (myotubes) observed after 9 days of myogenic induction, visualized by phase contrast microscopy, original magnification: 20, scale bar 50 m ( ). Immunofluorescence staining of MHC in the obtained multinucleated cells. The obtained myotubes showed a high positivity of MHC protein, ( ) negative control, myotubes stained with secondary antibody only, ( ) myotubes stained with anti-MHC antibody, LSCM: red for MHC and green for nuclei, original magnification: 10, scale bar 200 m. ( , ). Western blot analysis of MHC during myogenic differentiation. The analysis showed an increase in the gene during myogenic differentiation. Experiment was carried out in triplicate and is representative of the three established hSC lines ( ).",yes
PMC3717027,Figure_7,oa_package/30/76/PMC3717027.tar.gz,['PCOLCE sub-cellular localisation in OPMD and control muscle biopsies.'],Figure 7 Muscle cryosections were co-stained for PABPN1 (red; 3F5-VHH) and PCOLCE (green; 3F5-VHH). Nuclei were counterstained with DAPI (blue). Arrows point to nuclear PCOLCE that colocalizes with aggregated PABPN1. ( ) The sarcolemma is highlighted by staining for dysferilin (purple). Scale bar equals 10m. Immunofluorescence after KCl treatment. Treatment with 1M KCl disturbs localisation of soluble proteins whilst aggregates remain intact (arrow). Co-localisation between PABPN1 and PCOLCE is shown in the merged image. Scale bar equals 5m. Changes in PCOLCE sub-cellular localisation shown at low magnification. PABPN1 and DAPI mark nuclei. Scale bar equals 30m.,yes
PMC10963302,Figure_5,oa_package/b0/40/PMC10963302.tar.gz,['Enrichment analyses of aberrantly spliced transcripts.'],Figure 5 Enrichment analyses of aberrantly spliced transcripts. The 302 differentially spliced transcripts detected via Leafcutter were annotated using Metascape (A) and EnricherKG (B). Enriched terms based on their respective sources are shown.,yes
PMC6559989,Figure_7,oa_package/d1/93/PMC6559989.tar.gz,"['a c shows an attenuation of the fibrosis signature in lung, kidney, and muscle tissues from Clcn7G213R-specific siRNA-treated compared to scrambled siRNA-treated ADO2 mice, as analyzed by RNA-dSeq.', '7d, e), kidney (', '7f, g), and muscle (', '7h, j) perivascular fibrosis in Clcn7G213R-specific siRNA-treated compared to scrambled siRNA-treated ADO2 mice.', 'Effect of Clcn7G213R-siRNA treatment on fibrosis.', 'Images are representative, and data are the mean SD of five mice per group (Student s t test)Effect of Clcn7G213R-siRNA treatment on the cellular defects.']","Fig. 7 Effect of -siRNA treatment on fibrosis. A 10-day-old WT and ADO2 C57BL6/J male mice were treated with 4mg/kg of scrambled- (SCR) or -siRNA 3 times a week for 4 weeks. At the end of the experiments, mice were sacrificed and organs were harvested and used to extract RNA. Enrichment plots for fibrosis gene signature generated from ADO2 lung kidney and muscle RNA-dSeq data sets NES normalized enrichment score. Three-month-old WT and ADO2 C57BL6/J male mice were treated as described in ( ) for 12 weeks. At the end of the experiment mice were sacrificed and organs were harvested, fixed, and embedded in paraffin. Masson trichrome staining of lungs. Quantification of the perivascular collagen (blue) accumulation. Masson trichrome staining of kidneys. Quantification of the perivascular collagen (blue) accumulation. Masson trichrome staining of muscles. Quantification of the perivascular collagen (blue) accumulation. Images are representative, and data are the meanSD of five mice per group (Students test)",yes
PMC3378776,Figure_1,oa_package/cf/cd/PMC3378776.tar.gz,"['The induction of type 2 cytokine expression following the pulmonary instillation of allergen () was accompanied by increased mRNA expression of Il25 and Il17rb (', '1c) and tracked with the severity of the developing disease as depicted by histology (a).', 'Neither Ifn , other IL-17 family members, Il33, nor its receptor Il1rl1 were upregulated with our model of cockroach allergen challenge (b,c), indicating that type 2 inflammation represents the dominant response induced by this model.', 'While CD4+ T cells are present following antigen sensitization, the most numerous IL-17RB+ IL-4/IL-13 producing population in the lung were CD11b+ myeloid cells (d,e).', 'Lin ckit+ Sca1+ IL-17RB+ cells comprised a relatively rare population in the lungs of both na ve and allergen-challenged mice (range 250 1000), and were not increased following allergen sensitization (d,e).', 'Myeloid IL-17RB+ cells represented the major NBNT IL-4/13 cytokine producing population in the lung, outnumbering cytokine producing CD4+ T cells 68:1 (see Supplemental ).', 'Analysis of IL-4 and IL-13 production in IL-17RB+ myeloid subsets, based on levels of Gr-1 expression, allowed for the identification of two distinct IL-17RB+ myeloid populations (f).', 'A comparison of total cell numbers between na ve and allergic animals identified significant increases in both Gr-1mid (g) and Gr-1hi subsets in the lungs of allergic mice, however the IL-17RB+ CD11b+ Gr-1mid population produced IL-4 and IL-13 while the Gr-1hi population did not (data not shown).', 'Isolation of the Gr-1mid subset by FACS identified a granulocytic population with a circular partially segmented nucleus and relatively high nucleus to cytoplasm ratio (h, see Supplemental ', 'Respir Res200671351708372644DrewsACNeutrophilic airway inflammation is a main feature of induced sputum in nonatopic asthmatic childrenAllergy200964159716011939298645KikuchiSNagataMKikuchiIHagiwaraKKanazawaMAssociation between neutrophilic and eosinophilic inflammation in patients with severe persistent asthmaInt Arch Allergy Immunol2005137Suppl 17111594747846FukakusaMOral corticosteroids decrease eosinophil and CC chemokine expression but increase neutrophil, IL-8, and IFN-gamma-inducible protein 10 expression in asthmatic airway mucosaJ Allergy Clin Immunol20051152802861569608247LindenAHoshinoHLaanMAirway neutrophils and interleukin-17Eur Respir J2000159739771085386948JatakanonANeutrophilic inflammation in severe persistent asthmaAm J Respir Crit Care Med1999160153215391055611649Vazquez-TelloAInduction of glucocorticoid receptor-beta expression in epithelial cells of asthmatic airways by T-helper type 17 cytokinesClin Exp Allergy201040131213222054570850McKinleyLTH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in miceJ Immunol2008181408940971876886551KaikoGEPhippsSAngkasekwinaiPDongCFosterPSNK Cell Deficiency Predisposes to Viral-Induced Th2-Type Allergic Inflammation via Epithelial-Derived IL-25J Immunol185468146902085588152BerlinAAHogaboamCMLukacsNWInhibition of SCF attenuates peribronchial remodeling in chronic cockroach allergen-induced asthmaLab Invest2006865575651660738053CampbellEMKunkelSLStrieterRMLukacsNWTemporal role of chemokines in a murine model of cockroach allergen-induced airway hyperreactivity and eosinophiliaJ Immunol199816170477053986274254CampbellEHogaboamCLincolnPLukacsNWStem cell factor-induced airway hyperreactivity in allergic and normal miceAm J Pathol1999154125912651023386355LukacsNWRespiratory virus-induced TLR7 activation controls IL-17-associated increased mucus via IL-23 regulationJ Immunol2010185223122392062495056KallalLEHartiganAJHogaboamCMSchallerMALukacsNWInefficient lymph node sensitization during respiratory viral infection promotes IL-17-mediated lung pathologyJ Immunol2010185413741472080542257SmitJJThe balance between plasmacytoid DC versus conventional DC determines pulmonary immunity to virus infectionsPLoS One20083e172018320041Allergen exposure increases pulmonary IL-25 and IL17RB, and recruits bone marrow-derived IL17RB+ IL-4 and IL-13 producing myeloid cells to the lungAllergen-induced inflammation was localized to the lungs of C57BL/6J mice (n = 3 per group) via a series of 6 allergen challenges.']","Figure 1 Allergen exposure increases pulmonary IL-25 and IL17RB, and recruits bone marrow-derived IL17RB IL-4 and IL-13 producing myeloid cells to the lung Allergen-induced inflammation was localized to the lungs of C57BL/6J mice ( = 3 per group) via a series of 6 allergen challenges. ( ) Time course of representative PAS staining, taken 6 hrs post indicated allergen challenge. Upper row scale bar, 400 m; lower row, 100 m. ( ) Time course of pulmonary IL-4, IL-5, IL-13, IFN- and IL-17a mRNA expression 6 hours post indicated allergen challenge. (* < 8.44E , # = 0.0008, + = 0.001). ( ) Time course of IL-25, IL17RB, IL-33, IL-17b, and IL17d mRNA expression 6 hours post indicated allergen challenge. ST2 and IL-22 transcripts were not detectable. (* < 0.007). ( and ) IL-17RB lung subsets from nave and allergen sensitized C57BL/6J mice ( = 5 per group) were assessed by flow cytometry for IL-4 and IL-13 production. ( ) Representative flow plots of intracellular cytokine staining in IL-17RB CD11b Gr-1 cells from nave and allergen sensitized C57BL/6J mice. Gray: n-1 staining, black: nave IL-17RB CD11b Gr-1 , red: allergen challenged IL-17RB CD11b Gr-1 ( ) Pulmonary IL-17RB CD11b Gr-1 populations are significantly increased following allergen sensitization. (* = 0.0026). Results are representative of two independent experiments. ( ) Morphology of myeloid cells isolated from the lungs of allergen-sensitized mice. Cells were sorted as CD11b Gr-1 FcR IL-17RB CD4 CD8 B220 IL-7R Sca1 c-kit and stained with H+E. Scale bar 50 m. All data are presented as mean s.e.m.",yes
PMC8310171,Figure_2,oa_package/e4/b6/PMC8310171.tar.gz,"['At first glance, such conclusion appears also striking when looking at pictorial representations of the neural spreading pathways of PrPTSE and SynPD pathologies suggested to operate in peroral prion infections [32,48] and PD [15,74,75], respectively ().', 'Pictorial representation of the neural gut brain axis thought to be involved in the centripetal spread of pathological protein aggregation from the intestine to the CNS in both peroral prion infections as well as PD.']","Figure 2 Pictorial representation of the neural gutbrain axis thought to be involved in the centripetal spread of pathological protein aggregation from the intestine to the CNS in both peroral prion infections as well as PD. As established in great detail for perorally acquired TSE early spread of prions and PrP pathology to the CNS occurs from ENS ganglia in a retrograde direction along parasympathetic and sympathetic efferents of the vagus and splanchnic nerves, to the DMV in the medulla oblongata and the IML in the spinal cord, respectively (reviewed in: [ , ]). A similar concept was subsequently proposed for the propagation of LP in PD from enteric ganglia in the gastrointestinal tract to the CNS, initially with respect to parasympathetic and later also with respect to sympathetic spreading pathways [ , , ]. Dashed blue and red arrows indicate retrograde spread via efferent parasympathetic or sympathetic projections of the vagus or splanchnic nerves, respectively, for both peroral prion infections and PD. Dashed grey arrows mark spinal ascension of PrP pathology or LP to the brain following invasion of the IML at thoracic spinal cord levels of splanchnic innervation [ , ]. The figure was produced, with modifications, following templates from McBride et al. [ ] and Mabbott and MacPherson [ ]. Credits for mounted image components: Blausen 0838 Sympathetic Innervation and Blausen 0703 Parasympathetic Innervation by Bruce Blaus are licensed under the Creative Commons Attribution 3.0 Unported License (CC BY 3.0); Anatomic structure of enteric plexus (image ID FK8KPF) is licensed by Alamy Limited (Abingdon, UK, invoice number IY01945617). AP, area postrema; CMG, celiac or mesenteric ganglion; DMV, dorsal motor nucleus of the vagus nerve; DRG, dorsal root ganglion; ENS, enteric nervous system; IML, intermediolateral cell column; L, border between thoracic and lumbar spinal cord; MO, medulla oblongata; NG, nodose ganglion; SN, splanchnic nerves; STN, solitary tract nucleus; T, border between cervical and thoracic spinal cord; VN, vagus nerve.",yes
PMC10870049,Figure_1,oa_package/0e/8b/PMC10870049.tar.gz,['Violaceous papulonodular lesions on the right medial plantar surface.'],Figure 1 Violaceous papulonodular lesions on the right medial plantar surface.,yes
PMC6192235,Figure_1,oa_package/90/a3/PMC6192235.tar.gz,"['[78911121314151617181920](a) Tinea capitis in a child showed the presence of patch of hair loss with scaly and erythematous scalp.', '[24252728]1(a) Trichoscopic picture for a child with trichotillomania showed hair broken at different levels, trichoptilosis (red arrows), and flame hairs (green arrows).', 'In addition, some cases showed follicular pustules and inflammation along the margin of alopecia [2].', '2Traction alopeciaThis study showed that the trichoscopic features of traction alopecia were similar to those of trichotillomania.', 'However, flame hairs and coiled hairs are less common [3].', '3Trichoscopy of a child with traction alopecia showed reduction in the hair density, perifollicular erythema, and cast formation (red arrows)DISCUSSIONHair loss is generally a common and distressing problem in dermatology clinics.']",Figure 1 (a) Tinea capitis in a child showed the presence of patch of hair loss with scaly and erythematous scalp. (b) Tinea capitis showed patch of hair loss with scales but no erythema and with the prominent black dots within the patch of hair loss,yes
PMC3804955,Figure_2,oa_package/10/e9/PMC3804955.tar.gz,"['1 C and A).', 'Notably, BRD2 and BRD4 have nonredundant functions in TH17 cells, as knocking down either transcript individually was sufficient to recapitulate the phenotype observed with JQ1 or with double RNA interference ( B).', 'BET bromodomains are necessary for the transcription of multiple TH17 lineage-associated cytokines, including the TH17 autocrine amplification factor IL-21We consistently observed a complete suppression of IL-21 transcript and protein in JQ1-treated T cells, and in fact as early as 4 h after naive T cell culture under TH17 differentiating conditions, Il21 was one of the top 20 transcripts most down-regulated upon BET inhibition (unpublished data).', ', 2007), exogenous IL-21 was sufficient to induce its own transcription ( C).', 'This response was dependent on functional BET bromodomains, as JQ1 completely suppressed IL21 expression ( C).', 'Moreover, BET bromodomains were also essential for the background IL21 expression observed in the absence of added IL-21 protein ( C, compare lanes 1 and 3).', 'STAT3 activation downstream of the IL-6 receptor was not affected by BET inhibition, as JQ1 had no impact on STAT3 phosphorylation in naive cells incubated with IL-6 and TGF- for 90 min ( D, top).', 'However, BET inhibition resulted in a severe impairment of STAT3 activation and ROR t induction in cells examined after 5 d of culture under TH17-differentiating conditions (, D [bottom] and F [top left]), without affecting STAT3 expression (', 'Addition of exogenous IL-21 almost completely restored STAT3 activation and ROR t expression (, D [bottom] and F [top left]), demonstrating that by directly regulating IL-21 mRNA transcription, BET controls a fundamental amplification loop that contributes to the expression of ROR t and therefore drives optimal TH17 differentiation.', ', 2012), was also rescued with exogenous IL-21 ( F).', 'Although the addition of IL-21 circumvented the need of BET to restore RORC and BATF expression, it did not rescue IL-17 production ( F), indicating that BET also directly controls the expression of IL-17.', 'BET bromodomains played no role in the control of the expression of the generic proinflammatory cytokine TNF, as JQ1 did not affect its transcript levels ( F).', 'JQ1 blocked IL-17 production in a dose-dependent manner (not depicted), approaching full suppression at a concentration of 150 nM ( G).', 'In keeping with the notion of a direct role of BET in controlling multiple effector TH17-enriched cytokines, JQ1 also abrogated IL-21 and GM-CSF production ( G).', 'In contrast, the ability of these cells to produce TNF protein was only modestly ameliorated ( H, left) and not reduced at the transcript level (', 'Moreover, another TNF superfamily member, lymphotoxin- , was similarly unaffected ( H, right).', 'Furthermore, JQ1 had no effect on the expression of Il6r (unpublished data), complementing the data reported above ( D, top) for a role of BET in TH17 differentiation independent of early events in IL-6 signaling.', 'Of note, BET inhibition did not result in c-Myc transcript reduction ( A and not depicted).', 'BET members Brd2 and Brd4 directly bind to the Il17 genomic locus in a bromodomain-dependent mannerOur observation of a requirement of functional BET bromodomains for IL-17 mRNA generation (, A and F) prompted us to mechanistically elucidate whether the two BET family members expressed in TH17 cells, Brd2 and Brd4, associated with chromatin at the Il17 locus.']","Figure 2. (A) Human naive T cells were cultured under T 17 conditions in the presence of 150 nM JQ1 or DMSO for 48 h and the expression of the indicated genes was investigated by qPCR. Error bars represent standard deviation. Data are representative of two to three independent experiments. Statistical significance was determined by Students test (**, P < 0.01). (B) Human T cells were lentivirally transduced with hairpins targeting (red bars) or (blue bars) and cultured under T 17-polarizing conditions for 6 d. Two individual hairpins per gene were used (sh1 and sh2). Expression of , and was measured by qPCR; NTC, nontargeting control. IL-17A, IL-17F, and IL-21 protein was quantitated by Luminex. Data are representative of two independent experiments. Error bars represent standard deviation. Statistical significance was determined by Students test (*, P < 0.05; **, P < 0.01). (C) Human naive T cells were stimulated with anti-CD3/CD28 for 24 or 48 h in the presence or absence of exogenous human recombinant IL-21 and JQ1 as indicated, and expression was measured by qPCR. Data are representative of two independent experiments and two independent donors. Error bars represent standard deviation. (D) Human naive T cells were cultured under T 17-differentiating conditions for the indicated times in the presence or absence of exogenous human recombinant IL-21 and JQ1 as indicated, and phospho-Stat3 was measured by flow cytometry. Data are representative of two independent experiments and two independent donors. (E and F) Samples from D (5 d, bottom) were analyzed by qPCR for the indicated transcripts. Data are representative of two independent experiments and two independent donors. Error bars represent standard deviation. Statistical significance was determined by Students test (*, P < 0.05; **, P < 0.01); NS: not significant. (G and H) Human naive T cells were cultured under T 17 conditions for 6 d. After that time, cells were washed and rested for 16 h prior restimulation with anti-CD3/anti-CD28 for 48 h in the presence of DMSO control or 150 nM JQ1. Cytokine levels (luminex) or transcript levels (qPCR) are shown. Data are representative of two independent experiments. Error bars represent standard deviation. Statistical significance was determined by Students test (*, P < 0.05).",yes
PMC3271449,Figure_1,oa_package/19/6a/PMC3271449.tar.gz,"['Although partially filled, the bladder revealed a mass [].', 'Pelvic ultrasound showing a well-defined hypoechoic lesion noted at the anterior wall of the urinary bladder measuring 1.']",Figure 1 Pelvic ultrasound showing a well-defined hypoechoic lesion noted at the anterior wall of the urinary bladder measuring 1.4 1.2 1.2 cm,yes
PMC6244601,Figure_4,oa_package/36/70/PMC6244601.tar.gz,"[' 4a d) are common features of nonfacial AK, with homogenous brown pigmentation for the pigmented variant.', ' 4e), whereas the pigmented variant is ambiguous and frequently requires histology to exclude a diagnosis of hypomelanocytic melanoma or pigmented BCC.', '[31]): linear pigmented structuresNonsurgical methods could be considered, but PDT should be avoided for pigmented lesionsInvasive SCC/keratoacanthomaNodularWhite circles, white color polymorphic/thrombosed vesselsSurgeryAK actinic keratosis, BCC basal cell carcinoma, PDT photodynamic therapy, SCC squamous cell carcinomaaSurgical ablation such as curettage or cryotherapy\n', 'Bowen s disease photograph (c) and dermoscopic image showing yellowish scales (arrows) (d) and glomerular vessels at high magnification (e) (Renato Bakos; personal images)\nConfocal Microscopy: DescriptionConfocal microscopy or RCM is a noninvasive technique that enables real-time examination of skin tumors at cellular-level resolution (0.']",Fig.4 Nonfacial actinic keratosis and Bowens disease (adapted from Reinehr et al. [ ]). Photographs of nonfacial pigmented actinic keratosis lesions on the dorsa of the hand ( ) and the corresponding dermoscopic image (original magnification20) with opaque white scales (arrows) and homogenous brown pigmentation ( ) (from Reinehr et al. [ ]). Bowens disease photograph ( ) and dermoscopic image showing yellowish scales (arrows) ( ) and glomerular vessels at high magnification ( ) (Renato Bakos; personal images),yes
PMC10607091,Figure_1,oa_package/b9/1a/PMC10607091.tar.gz,"['Defining Glomerular Injury Patterns and Datasets for ClassificationAll glomerular images that were extracted were reviewed and categorized into nine injury patterns: mesangioproliferative, endocapillary, membranoproliferative, membranous, crescentic, segmental glomerulosclerosis, hypertrophy, global glomerulosclerosis or normal glomeruli ().', '003Examples of glomerular patches containing different patterns of injury used to train the classifiers.']","Figure 5 ROC curves and means of AUC scores by class for each classification experiment. The blue curve indicates the performance of the single-multiclass classifier, the orange curve indicates the performance of the multiple-binary classifier and the green curve indicates the spatially guided CNN classifier.",yes
PMC8364553,Figure_6,oa_package/ae/fb/PMC8364553.tar.gz,"['6A).', '6B, C).', '6D).', '6E, F).', 'CPT1 regulates respiration and ATP production in cultured tubular epithelial cells.', 'Tubular cells with reduced CPT1 are more sensitive to folic acid-induced alternationWe next provided specific evidence by measuring the effect of CPT1 siRNA on lipid accumulation and tubular cell phenotype in folic acid treatment conditions.']","Fig. 6 CPT1 regulates respiration and ATP production in cultured tubular epithelial cells. Western blot analysis of protein expression of CPT1 in cultured tubular cells after transfection with N.C. or CPT1 siRNA. Representative traces show OCR in tubular cells. Summary OCR data from 5 to 6 independent experiments are shown. * <0.05 versus N.C. Western blot analysis of protein expression of CPT1 in cultured tubular cells after transfection with pcDNA3 or CPT1 plasmid. Representative traces show OCR in tubular cells. Summary OCR data analyzed from 5 to 6 independent experiments are shown. * <0.05 versus pcDNA3. One mol/L Oligomycin, 0.75 mol/L of FCCP, 1 mol/L of antimycin A and rotenone were added where indicated.",yes
PMC4960210,Figure_1,oa_package/ab/f5/PMC4960210.tar.gz,"['An autopsy revealed evidence of lymphomatous infiltration of the brachial and lumbosacral plexus (A and ', 'Gadolinium-enhanced MRI revealed localized longitudinal thickening of the right sciatic nerve with contrast enhancement in the mid-thigh region (C) while FDG-PET showed intense FDG uptake in the right sciatic nerve in the same region with involvement of bone marrow (', 'The potential role for prophylactic therapyAm J Med19796643544343395013ChesonBDRole of functional imaging in the management of lymphomaJ Clin Oncol201129184418542148298214SalmLPVanderStokkelMPIncreasing importance of 18FFDG PET in the diagnosis of neurolymphomatosisNucl Med Commun2012339079162271400615JungYHWooISHanDJHanCW(18)F-fluoro-2-deoxy-D-glucose positron emission tomography findings of neurolymphomatosisBlood Res201449832502500716CheungCLopesDHungKNChanTChanKWKwongYLNeurolymphomatosis: role of positron emission tomography in diagnosisAnn Hematol201291131313142213056217HongCMLeeSWLeeHJSongBIKimHWKangSNeurolymphomatosis on F-18 FDG PET/CT and MRI findings: a case reportNucl Med Mol Imaging201145767824899982Nerve bundle infiltration (patient 1), FDG-PET and MRI of right sciatic nerve (patient 2).']","Fig. 1 Nerve bundle infiltration (patient 1), FDG-PET and MRI of right sciatic nerve (patient 2). A: A nerve bundle with infiltration by lymphoma cells (patient 1). B: CD 20 stain highlights diffuse infiltration by diffuse large B cells (patient 1). C: PET CT showing intense FDG uptake in right sciatic nerve (yellow arrow) (patient 2). D: intense enhancement of right sciatic nerve (arrow) on T1 weighted contrast MRI (patient 2).",yes
PMC11603663,Figure_5,oa_package/5c/ad/PMC11603663.tar.gz,"[' 5A; Additional file 4: ', ' 5B; Additional file 4: S12A) or ionized calcium-binding adapter molecule 1 (Iba1) (Additional file 4: ', ' 5C, D), suggesting gliosis.', ' 5C; Additional file 4: ', ' 5E).', ' 5E).', '', 'Scale bars: 50 m (A, top), 25 m (A, bottom), and 100 m (C)DiscussionIn this study, we aimed to investigate the impact of excessive ALS-linked mutant TDP-43 expression on oligodendrocyte pathology in mice.']","Fig.5 Apoptosis is induced in the oligodendrocytes of the white matter from ; hTDP-43 -fl*/fl mice. Representative immunofluorescence images of lumbar spinal cords from 12-month-old mice stained for APC, cleaved caspase-3 (Cc-3), and Mac2. Bottom panels show magnified images of the areas indicated by dashed squares. Some mature oligodendrocytes expressed Cc-3. Arrowheads indicate colocalization of apoptotic oligodendrocytes and activated microglia; arrows indicate apoptotic oligodendrocytes without microglial colocalization. Mean number of APC Cc-3 Mac2 and APC Cc-3 Mac2 cells per 0.1 mm was quantified and plotted (n=4 sections per mouse). Representative images of the corpus callosum and white matter of the lumbar spinal cord of 12-month-old mice immunostained for Myc, Iba1, and GFAP. Quantification of relative intensity to nTg per 0.1 mm in the corpus callosum and the white matter of the lumbar spinal cord in 12-month-old mice (n=4 sections per mouse). mRNA levels of representative microglia and astrocyte markers in 12-month-old mice were determined via quantitative PCR. Data are presented as meansSEMs; -values were determined using one-way ANOVA followed by TukeyKramer multiple comparison tests. Scale bars: 50m (A, top), 25m ( , bottom), and 100m ( )",yes
PMC3046155,Figure_12,oa_package/0f/e9/PMC3046155.tar.gz,[],10.1371/journal.pone.0017299.g012,yes
PMC11605946,Figure_2,oa_package/f3/db/PMC11605946.tar.gz,"[' 2a-b and Additional file 1: ', ' 2c and Additional file 1: ', ' 2d-j and Additional file 1: ', ' 2k), and reduced M1 and pro-inflammatory markers (', ' 2l and Additional file 1: ', '\nThe influence of cholesterol on macrophages polarization and inflammation in MASH.', '0001 between groups\nDownregulation of cholesterol metabolic enzyme DHCR7 in MASH-associated macrophagesGiven the association of cholesterol accumulation in MASH, we examined cholesterol-metabolizing enzymes in macrophages.']","Fig. 2 The influence of cholesterol on macrophages polarization and inflammation in MASH. ( ) Schematic and treatment setup of RAW264.7 macrophages with ox-LDL for 24h (cholesterol overload). ( ) Cellular TC and relative mRNA levels of , , and after ox-LDL treatment at 20, 50, or 100 ug/ml for 24h. ( ) Schematic and treatment setup of RAW264.7 macrophages with 100 ng/mL LPS for 24h (M1 polarization). ( ) Relative mRNA expression profiling for M1 polarization markers ( , and ) and pro-inflammatory cytokines ( , and ). ( ) Quantification of secreted IL-1, IL-6 and TNF-. ( ) Western blot analysis for M1-associated CD86 and M2-associated ARG-1 proteins, with densitometry. ( ) Immunofluorescence for CD86 and CD206 at 200 magnification. ( ) TC levels within RAW264.7 cells in M1 polarization. ( ) Effect of simvastatin (2.5 M or 5 M) co-treated with LPS (100 ng/mL) for 24h on intracellular TC and relative mRNA levels of inflammatory and polarization markers in cells. Data are presented as meanSD, with =3 per group. <0.05, <0.01, <0.001, and <0.0001 between groups",yes
PMC10571363,Figure_2,oa_package/d3/d3/PMC10571363.tar.gz,"[' 2A; for wake, treatment by day interaction F1,18 = 0.', ' 2B; h effects F23,184/230 12.', 'Wake/sleep architecture variables in A scr- and A o-injected rats during BL and INJ days.', 'A scr, n = 11; A o, n = 9Sleep fragmentation/consolidation is unaltered by A oFragmented sleep is characterized by shorter and more frequent individual bouts of states, whereas consolidated wake/sleep is associated with longer and less frequent bouts.', ' 2C; for wake, treatment by day interaction F1,18 = 0.']","Fig. 2 Wake/sleep architecture variables in Ascr- and Ao-injected rats during BL and INJ days. Percent time spent in wake, SWS, and PS during 24h at BL and INJ in the two groups. Percent time spent in wake, SWS, and PS computed per h for BL and INJ in each group. Twenty-four-h mean duration of individual bouts of wake, SWS, and PS for BL and INJ in each group. Ascr, =11; Ao, =9",yes
PMC3662312,Figure_5,oa_package/61/db/PMC3662312.tar.gz,[],"Figure 5 Pathological vacuolization in the brain of treated MPS-IIIA mice. A. Ultra-thin brain sections from the indicated experimental group of mice (7 months after the injection) were stained with toluidine blue to evaluate the extent of vacuolization (yellow arrowheads indicate extensively vacuolated cells). The graph below shows a semi-quantitative analysis of vacuolated cells generated by analysing 100 cells for each experimental group of mice at 1, 3 and 7 months after injection. Each bar represents the average of 3 injected mice (values are meansSEM). * <0.05 Student's -test (normal GFP MPS-IIIA SGSH, MPS-IIIA SGSH MPS-IIIA IDSspSGSH and MPS-IIIA SGSH MPS-IIIA IDSspSGSH-ApoB for each time-point). Scale bar, 30m. B. Cortex brain regions were fixed and prepared for EM as described in Materials and Methods. Asterisks in each image indicate the lysosomes. Panels on the left show lysosomes in the brain of control normal mice. MPS-IIIA cortex exhibits numerous large lysosome-like structures. Cortex from MPS-IIIA mice treated with AAV2/8-TBG containing the IDSspSGSH-ApoB-BD shows a reduced number of enlarged lysosomes. Scale bar 1m.",yes
PMC8702228,Figure_4,oa_package/1c/c1/PMC8702228.tar.gz,['The second recurrent lymph node showed that architecture was totally replaced by tumor cells arranged in mixed macro- and micro-follicular patterns without obvious papillary structure.'],Figure 4 The second recurrent lymph node showed that architecture was totally replaced by tumor cells arranged in mixed macro- and micro-follicular patterns without obvious papillary structure.,yes
PMC5563342,Figure_1,oa_package/ea/92/PMC5563342.tar.gz,"[' 1a, b) [252].', '', 'l Toluidine blue stain of a semithin section of the sural nerve shows demyelination with accruing of foamy macrophages\nThe degree of neuropathological changes in MLD depends on disease onset [214].', ' 1c).', ' 1d, e).', ' 1f, g).', ' 1h).', ' 1i).', ' 1j, k).', ' 1l).']","Fig.1 Metachromatic leukodystrophy. T2-weighted axial image of a 7-year-old child shows radiating stripes of tissue with preserved signal ( ). The U-fibers are spared. Follow-up T2-weighted axial image of the same child at 13years shows a diffuse, bilateral and symmetric signal hyperintensity in the cerebral white matter. The U-fibers are no longer spared and the stripes are less well visible. There is a mild atrophy. Sagittal cut of the brain of a 12-year-old child shows thinning of the corpus callosum and optic nerves. On coronal sections through the brain of a 6-year-old child, the demyelinated white matter appears grayish and gelatinous. Whole mount of a coronal section of a 10-year-old child stained with Luxol fast blue and Haematoxylin & Eosin shows diffuse loss of myelin in the frontal and temporal lobe with relative sparing of the U-fibers and internal capsule. Haematoxylin & Eosin stain of the peripheral cerebral white matter shows tissue pallor, loss of oligodendrocytes and presence of foamy macrophages and reactive astrocytes. Klver-Periodic acid Schiff (PAS) stain of the same area shows loss of myelin and diffusely distributed macrophages filled with PAS-positive granular material. Stain against the glial fibrillary acidic protein (GFAP) shows a moderate diffuse isomorphic astrogliosis. The inset shows a metachromatic macrophage stained with Toluidine blue. Haematoxylin & Eosin stain of the thalamus shows accumulation of storage material in the cytoplasm of neurons. , Stain against neurofilaments (NF) shows axonal swellings ( ) and dendritic varicosities ( ) in the cerebellar cortical Purkinje cells. Note also the marked dropout of granular neurons in ( ). Toluidine blue stain of a semithin section of the sural nerve shows demyelination with accruing of foamy macrophages",yes
PMC8163134,Figure_2,oa_package/d8/d9/PMC8163134.tar.gz,[],"FIGURE 2 Shortaxis view of the perimembranous ventricular septal defect, adjacent to the commissure of the right and noncoronary cusp, of the same horse as in Figure . The sinus of Valsalva (arrow) and opened aortic valve cusp (arrowhead) are shown. The double arrow shows the pmVSD shortaxis diameter. Ao, aorta; LA, left atrium; pmVSD, perimembranous ventricular septal defect",yes
PMC3564530,Figure_8,oa_package/26/7b/PMC3564530.tar.gz,"['We observed a dramatic enhancement in CIN exon 2/poly-Ub and full-length BGIN/poly-Ub interactions in the APP-expressing cell line compared with nonexpressing cells (A and Supplemental S8B).', 'We also found a slight increase in membrane-associated BGIN and a dramatic reduction in Rac1 activation and cofilin phosphorylation with APP expression (B).', 'We next determined whether BGIN depletion could modulate Rac1 activity in normal or transgenic APP contexts.', 'However, we found that long-term BGIN siRNA treatment (continuous siRNA transfection over 4 6 d; see the Supplemental Methods) produced a 50% increase in Rac1 activity in an APP-transgenic background, with little change in SH-SY5Y cells (C and Supplemental S8G).', 'In agreement with the Rac1-GTP profile observed, APP cells also demonstrated a concomitant increase in ROS output with long-term BGIN siRNA treatment (D).', 'These results contribute additional evidence that poly-Ub/BGIN interactions may contribute to downstream Rac1 signaling with neurodegenerative proteotoxicity (E).', 'We describe here a novel poly-Ub binding domain, the CIN exon 2 module, which exhibits specificity for poly-Ub chains and mediates the distribution of BGIN to membranes to limit membrane-associated Rac1 activity (E).']","FIGURE 8: Determining a role for BGIN in Rac1 signaling with APP proteotoxicity. (A) Probing poly-Ub/CIN exon 2 interactions in APP-expressing SH-SY5Y cells. GST or GST-CIN exon 2 was expressed and precipitated from untransfected or APP-expressing SH-SY5Y cells as indicated and immunoblotted for GST or Ub. Graph indicates poly-Ub smear band density normalized to GST-CIN exon 2 precipitates from three experiments. (B) Stable APP expression attenuates Rac1 activity. Top, normal or APP-expressing SYSH5Y cells were processed to generate total, cytosolic, membrane, or insoluble fractions and immunoblotted. Bottom, total lysates were assayed for Rac1 activity by PBD pull-down assay or cofilin phosphorylation with or without APP expression. (C) Long-term BGIN siRNA treatment elevates Rac1-GTP levels in APP-expressing cells. Normal or APP-expressing SH-SY5Y cells were transfected for three rounds of siRNA as described in the Supplemental Methods and subjected to Rac1-GTP analysis by PBD pull-down assay. Fold change in Rac1-GTP levels with long-term BGIN siRNA treatment in comparison to controls (set to 1.0) were calculated in the lower bar graphs. (D) Cells were transfected as described in C and subjected to chemiluminescence assay for ROS output. Kinetic ROS output values are shown in the upper line graph, and relative ROS values from three experiments measured in triplicate are depicted in the lower bar graph. All bar graph values (BD) represent mean SE from a minimum of three experiments (** < 0.01, *** < 0.002). Paired test values were tabulated for C (* < 0.02). (E) Left, schematic summary of poly-Ub/BGIN interactions and consequent effects on Rac1/ROS generation. Right, experimental summary of results supporting the key features of the model.",yes
PMC11410999,Figure_6,oa_package/40/4c/PMC11410999.tar.gz,"[' 6A).', '', '01To assess the therapeutic effectiveness of combining hepsin with a reduced dose of 200 mg/kg NAC (', ' 6B), we measured serum AST and ALT levels in the early stage post-APAP administration to evaluate protection against 600 mg/kg APAP-induced hepatotoxicity.', ' 6C).', ' 6D).', ' 6E).']","Fig.6 Combination of AAV-mediated liver-specific administration of hepsin and post-injection with N-acetyl-cysteine has a superior therapeutic effect compared with N-acetyl-cysteine treatment alone ( ) The survival rate after 600mg/kg APAP administration, followed by different doses of NAC therapy provided 1h post-APAP treatment. ( ) Experimental timeline. Adult wild-type mice were administered AAV-hHPN , AAV-hHPN , or AAV-EGFP for 3weeks, followed by 600mg/kg APAP treatment for 1h, after which the mice were treated with NAC. ( ) Measurement of serum AST and ALT at 20h after APAP treatment (n=35 per group). ( ) Images of liver pathology in sections assessed by hematoxylin and eosin staining at 20h after APAP treatment. ( ) Survival rate. In the survival analysis, sample sizes for each group are indicated in brackets, and statistical significance was determined using the log-rank test, with significance levels represented by asterisks: < ** <0.01, < . Data are presented as the meanSD in bar charts, with significance levels denoted by asterisks: ** <0.01",yes
PMC6837823,Figure_3,oa_package/da/8d/PMC6837823.tar.gz,[],FIGURE 3 (a) Mammogram of a 56-year-old woman with a large cancer in the posterior outer aspect of the right breast. There is asymmetry and subtle distortion anterior to the mass (white arrow). This is suspicious for intra-ductal extension (associated ductal carcinoma in situ). The extent on mammogram measures 6 cm. (b) Post-contrast magnetic resonance imaging axial maximal intensity projection (MIP) shows extensive segmental non-mass enhancement extending all the way from the posteriorly situated mass to the retroareolar space. Extent is 11 cm.,yes
PMC3857475,Figure_1,oa_package/46/20/PMC3857475.tar.gz,"['Indeed, endogenous Fus, but not hnRNPA2, associates with immunoprecipitated APC ( a).', 'Additionally, immunoprecipitated GFP-Fus associates specifically with APC, but not with -catenin ( b), indicating that Fus is not part of the destruction complex in the Wnt pathway.', 'Furthermore, Fus associates with RNAs that are present in APC-RNPs (Pkp4, Rab13, Kank2; c; Mili et al.', '.', 'To test whether Fus is present in protrusions, we isolated protrusions and cell bodies from cells induced to migrate on microporous filters ( d).', 'Indeed, Fus was present within protrusions, whereas Ddx5, a nuclear shuttling RNA-binding protein analogous to Fus, was not ( d).', 'We additionally immunostained actively spreading cells using different Fus antibodies ( e and ', 'To enhance our ability to detect cytoplasmic Fus, the cells were permeabilized with digitonin to prevent antibody access into the nucleus, where most of Fus resides at steady state ( f; Dormann et al.', 'Fus was detected in the cytoplasm concentrated in peripheral granules that colocalized with APC ( e and ', 'Similar results were obtained with exogenous GFP-Fus ( f).', 'Given that cytoplasmic Fus is present at cell protrusions (, d and e; and ', 'However, taken together the above data strongly indicate that Fus is preferentially required for efficient translation within cell protrusions, consistent with its physical enrichment there ( e).', 'S1 shows (a) Fus peptides identified through mass spectrum analysis (related to a), (b) quantifications of Fus-RNA coimmunoprecipitations (related to ', '1 c), (c and d) additional examples of Fus-APC colocalization at protrusions (related to e), and (e and f) effects of Fus knockdown on RNA localization at protrusions (related to ']","Figure 1. NIH/3T3 cells untransfected (a and c) or transfected with GFP or GFP-Fus (b) were immunoprecipitated (IP) with the indicated antibodies and analyzed by Western blot (ac, top panel) or by RT-PCR (c, bottom panels). (d) NIH/3T3 cells were plated on microporous filters, induced to migrate by addition of LPA, and protrusions and cell bodies were isolated and analyzed by Western blot. (e) NIH/3T3 cells were immunostained to detect endogenous Fus and APC. Insets: magnification of the boxed protrusive area. Yellow line in overlay panel: cell outline (f) GFP-Fusexpressing cells immunostained to detect APC. Bars, 10 m (insets, 3 m).",yes
PMC10108032,Figure_2,oa_package/44/46/PMC10108032.tar.gz,['Co expression of fibroblast and adipocyte markers suggest lipomatosis is driven by adipogenic transdifferentiation of medullary fibroblasts.'],"Figure 2 Coexpression of fibroblast and adipocyte markers suggest lipomatosis is driven by adipogenic transdifferentiation of medullary fibroblasts. Immunofluorescence staining of lymph nodes (LNs) with lipomatosis. (A) Presence of adipocytes (perilipin, magenta) and fibroblasts (alpha smooth muscle actin; SMA, green) in medullary cords. Arrowheads indicate adipocytes present in the layer of medullary lymphatic sinus lining fibroblasts. Scale bar: 100m. Pictures representative of 10 analyzed LNs. (B) Colocalization of SMA (green) and perilipin (magenta) in medullary fibroblasts. Scale bar: 50m. Picture representative of 10 analyzed LNs. Inset shows vector (size: 4.6m) used for image analysis of SMA and perilipin signal colocalization with vectorbased profile plot of SMA and perilipin. Black line denotes position of peak of perilipin in both profiles. (C) Colocalization of PDGFR (green) and perilipin (magenta) in medullary fibroblasts. Scale bar: 20m. Picture representative of five analyzed LNs. Inset shows vector (size: 7.0m) used for image analysis of PDGFR and perilipin signal colocalization with vectorbased profile plot of PDGFR and perilipin. Black line shows position of peak of perilipin in both profiles.",yes
PMC8472374,Figure_1,oa_package/30/36/PMC8472374.tar.gz,"['With respect to HGG, PCNSL shows lower signal on T2-weighted images, less necrosis, more intense contrast enhancement, lower rCBV values, higher PSR, T1-dominant leakage pattern, and low ADC within the solid part of the tumor ( and ) [7,8,9,10].', '2% of cases ( and ).', '1093/neuonc/noab10634185076Neuroradiological and histological features of PCNSL.']","Figure 1 Neuroradiological and histological features of PCNSL. ( ) Axial T2-weighted and ( ) axial Gd-enhanced T1-weighted MR imaging showing a T2 hypointense mass involving splenium of the corpus callosum, without necrosis, and with vivid contrast enhancement. DWI ( ) showing restricted diffusion and SWI ( ) showing absence of intratumoral hemorrhage. Arrows point at the tumor ( ). CBV map ( ) shows moderately increased intratumoral rCBV. The neuropathological examination reveals a high-grade diffuse B cell non-Hodgkin lymphoma ( ) showing strong CD20 positivity ( , inset).",yes
PMC9791521,Figure_3,oa_package/63/40/PMC9791521.tar.gz,['\nHistopathological examination.'],Figure 3 Left breast cancer (white arrow) and left axillary sarcoma (orange arrow). A: Hematoxylin-eosin staining ( 100); B: Hematoxylin-eosin staining ( 40).,yes
PMC3142496,Figure_4,oa_package/aa/2e/PMC3142496.tar.gz,"['Comparison of normal lungs and pneumonia with necrotic areas and bacterial colonies in M.', 'A: The lung parenchyma of a partridge from the control group.', 'B: A focus of necrotic pneumonia with secondary bacterial colonisation (*) surrounded by a thin layer of heterophils and lymphocytes in a partridge infected with M.']",Figure 4 . Figure 4A: The lung parenchyma of a partridge from the control group. Parabronchial lumen (1) and wall with bundles of smooth muscle tissue (2) is surrounded by air and blood capillaries. H&E stain. Figure 4B: A focus of necrotic pneumonia with secondary bacterial colonisation (*) surrounded by a thin layer of heterophils and lymphocytes in a partridge infected with H&E stain.,yes
PMC4941200,Figure_4,oa_package/16/ba/PMC4941200.tar.gz,['The cecum was again identified in the middle of the abdomen and was noted to have a gangrenous appendix with several perforations as well as a large fecalith ().'],Fig. 4 Appendiceal perforation. The appendix (black arrow) was found to be gangrenous and perforated at its base.,yes
PMC7811721,Figure_2,oa_package/9d/04/PMC7811721.tar.gz,"['EUS images showed hypoechoic, heterogeneous lesions, with irregular margins, involving deep mucosa and submucosa, and even invading through the muscularis propria into the perirectal fat in one case [].', 'Recurrent rectal adenocarcinoma.']","Figure 2 Recurrent rectal adenocarcinoma. (a) Endoscopic findings: Moderate sized, firm, subepithelial mass with smooth mucosa in the rectosigmoid region; (b) EUS findings: Hypoechoic mass within the sigmoid/rectal wall, with intact mucosa. The mass was involving the muscularis propria, submucosa. The mass had an infiltrating appearance and appeared to be invading into perirectal fat",yes
PMC8924809,Figure_3,oa_package/53/9d/PMC8924809.tar.gz,"['001) ().', 'When BARD was used to define significant liver fibrosis, similar findings were obtained (Supplementary ).', 'ASCVD score and proportion of patients with high ASCVD risk according to NFS-defined significant liver fibrosis stratified by the CNS-defined NAFLD/obesity status.']","Fig. 3 ASCVD score and proportion of patients with high ASCVD risk according to NFS-defined significant liver fibrosis stratified by the CNS-defined NAFLD/obesity status. Lean subjects with NFS-defined significant liver fibrosis had a significantly higher ASCVD score (A) and prevalence of a high ASCVD risk (B) than obese subjects with significant liver fibrosis and those without significant liver fibrosis (all p<0.001). ASCVD, atherosclerotic cardiovascular disease; NAFLD, nonalcoholic fatty liver disease; NFS, NAFLD fibrosis score; CNS, comprehensive NAFLD score.",yes
PMC3626254,Figure_8,oa_package/77/2c/PMC3626254.tar.gz,"['89There is increasing recognition that femoral version can exacerbate\nor mitigate the severity of\nFAI ().', '88,92Diagrams showing the proposed mechanism\nof the effect of femoral version.', 'The effect of femoral neck-shaft angle is even less clear.']","Fig. 8 Diagrams showing the proposed mechanismof the effect of femoral version. In the retroverted femur (left),the femoral head is already relatively rotated into the acetabulum,which decreases the clearance of any head-neck abnormality in flexionand exacerbates cam impingement. In an opposite manner, femoralanteversion (right) may mitigate the effect of an anterior cam deformitybut could result in more impact on the posterior rim in externalrotation. (Reprinted with permission: Femoralantetorsion: comparing asymptomatic volunteers and patients withfemoroacetabular impingement. 2012;263:475483).",yes
PMC5746521,Figure_4,oa_package/4e/5b/PMC5746521.tar.gz,['Haemin induces Hmox1 reporters in liver and kidney cellsTriplicate reporter mice were treated with 30 mg kg 1 haemin and killed 18 h later.'],"Figure 4 Haemin induces Hmox1 reporters in liver and kidney cells Triplicate reporter mice were treated with 30mgkg haemin and killed 18h later. All mice were male and aged between 19 and 20weeks except for one haemintreated mouse that was 14weeks old. , mice were imaged at baseline and again just before necropsy. The accompanying graph quantifies the luminescent signal. The effect of haemin was significant at <0.0005 (twoway ANOVA with repeated measures). , spleen, liver, kidney and large intestine (LI) sections from control and haemintreated mice were galactosidase stained. Representative images are shown. Scale bar=250m except LI (100m) and kidney (50m). , pooled organ lysates ( =3) were probed for the indicated proteins. GAPDH was used as a loading control. The Hmox1 present in the treated kidney sample migrates more rapidly than expected and probably represents a partially proteolysed product.",yes
PMC5596937,Figure_5,oa_package/17/0c/PMC5596937.tar.gz,"[' 5).', 'Immunohistochemical staining of INTS6 in HCC.', 'Different INTS6 staining intensities [negative: 0, weak: 1, moderate: 2, strong: 3] are indicated in the micrographs\nTable 1Correlation between expression level of INTS6 and clinicopathlogical features of HCC in Immunohistochemistryclinicopathological featuresINTS6 expressionTotal\nP-valuelowhighAll cases442670Age63 Open or arthroscopic repair provides good clinical results ().', 'Right hip (peritrochanteric endoscopic view).']",Fig. 6 Right hip (peritrochanteric endoscopic view). Gluteus medius (GM) tear (lower left corner image). Repair of GM with anchors to the greater trochanter (GT).,yes
PMC10599350,Figure_7,oa_package/77/5f/PMC10599350.tar.gz,"['In addition, O-glycosylation was most abundant in GCs at the bottom of intestinal crypts and on the mucosal surface of the intestine (A-F).', 'Variation in MUC2 and WGA in intestinal mucosa.']","Figure 7 Variation in MUC2 and WGA in intestinal mucosa. (Aa-p) IF showed MUC2 and WGA in ileum of WT and dTg mice at 6 and 12 months. (B) MUC2- and (C) WGA-positive area was determined by IF in ileum (n=6/group). IF showed MUC2 and WGA in (Da-p) proximal colon of WT and dTg mice at 6 and 12 months. (E) MUC2- and (F) WGA-positive area was determined by IF in proximal colon (n=6/group). P<0.05, P<0.01. WGA, wheat germ agglutinin; IF, immunofluorescence; MUC2, mucin 2; WT, wild-type; dTg, double transgene.",yes
PMC4516522,Figure_2,oa_package/a9/df/PMC4516522.tar.gz,[],"Figure 2 Measurement of contraction of mESC-CM in the different stages of differentiation. Representative traces of the influence of addition of 0.1 mM and 1.0 mM TC on the rate and amplitude of contraction of mESC-CM in the early (A), intermediate (B) and late (C) stages of differentiation.",yes
PMC7731859,Figure_2,oa_package/8e/ca/PMC7731859.tar.gz,"['A subcutaneous generator pocket was then created by an incision through the puncture point ().', '\n\nA subcutaneous generator pocket was created by an incision through the puncture point.']",Fig. 2 A subcutaneous generator pocket was created by an incision through the puncture point.,yes
PMC5510975,Figure_3,oa_package/e2/9d/PMC5510975.tar.gz,['Axial non-contrast computed tomography scan of the brainDemonstrating resolution of the tension pneumocephalus and decompression of the brain.'],Figure 3 Axial non-contrast computed tomography scan of the brain Demonstrating resolution of the tension pneumocephalus and decompression of the brain.,yes
PMC7752433,Figure_2,oa_package/30/4b/PMC7752433.tar.gz,"['B, Multiple violaceous papules on the backPunch biopsy showing mild epidermal hyperplasia above an inflammatory infiltrate in the upper dermis.']","Figure 2 Punch biopsy showing mild epidermal hyperplasia above an inflammatory infiltrate in the upper dermis. H&E staining, 1.25 magnification. Scale bar 100m. Insert: Higher power view of many neutrophils without vasculitis. H&E Staining, 40 magnification",yes
PMC11162840,Figure_4,oa_package/08/ba/PMC11162840.tar.gz,"['Also, multiple intraperitoneal metastases were confirmed ().', '.']","Fig. 4. Mixed adenoneuroendocrine carcinoma cell as histopathological examinations of peritoneal metastatic seeding lesion (hematoxylin-eosin, 100). In the neuroendocrine carcinoma area, the neoplastic cells show a trabecular growth pattern and rosette-like structures.",yes
PMC3752970,Figure_5,oa_package/8a/2b/PMC3752970.tar.gz,[],"Fig.3 Divergence of G2019S PD brains in levels of highly aggregated urea-soluble -synuclein species. The urea-soluble fractions of basal ganglia (A) and limbic cortex (B) of G2019S, iPD and control brains were analysed for the presence of -synuclein. Aggregated -synuclein is observed in the basal ganglia and the limbic cortex of the iPD samples following pathology severity. Very little aggregated synuclein is detected in the urea-soluble samples of the G2019S cases compared to the iPD cases. A dot-blot of urea-fractions of the basal ganglia (C) reveals much lower levels of -synuclein in the G2019S cases compared to that of the iPD samples, whilst no -synuclein is detected in controls. [ANOVA with Bonferroni's correction: * <0.01].",yes
PMC10836805,Figure_7,oa_package/6d/5d/PMC10836805.tar.gz,"['Quantitative PCR corroborated increased expression of specific marker genes of these populations in primary FBs derived from lesional HS versus NS skin (Supplemental A).', 'To uncover the functional role of Hippo signaling (A) in HS fibrosis, we performed ex vivo experiments using primary dermal FBs obtained from chronic HS lesions.', 'Verteporfin significantly reduced both protein and RNA expression of collagen I and, to a lesser extent, smooth muscle actin (SMA/ACTA2) in HS FBs (, B and C).', 'Verteporfin stimulation also significantly inhibited HS FB contractility in the gel contraction assays (D) and resulted in a significant dose-dependent reduction of both proliferation and migration of HS FBs (, E and F).', 'In contrast, stimulation of YAP transcriptional activity with TRULI resulted in a nonsignificant increase in RNA expression of smooth muscle actin (ACTA2) and collagen I (COL1A1) (B).', 'TRULI treatment did significantly induce CTGF expression (B).', 'Treatment with TRULI also significantly increased proliferation but failed to further increase either migration or gel contraction (, D F).', 'In particular, upregulation of this pathway by TRULI seemed to result in a more limited upregulation of collagen I or smooth muscle actin RNA and protein in comparison with HS FBs (B and Supplemental 1, A and B).', 'Overall, neither TRULI nor verteporfin significantly affected the expression of CCL2, CCL5, CXCL1, CXCL8, or IL6 in HS FBs in response to stimulation with the previously identified upstream regulators IL-1 , TNF, or IFN- (G).', 'Increased activation of this pathway has been shown to play a pivotal role in fibrotic diseases such as idiopathic pulmonary fibrosis (22), and our data further implicate this pathway in HS fibrosis ().', 'Central to Hippo signaling is a kinase cascade, wherein MST1/2 and SAV1 form a complex to phosphorylate and activate LATS1/2 (A) (48).', 'In HS FBs, treatment with verteporfin, which inhibits transcriptional activity of the Hippo pathway through disruption of the interaction between YAP/TAZ and TEAD1 4, reduced both the myofibroblast phenotype and collagen production, whereas the opposite response was seen with TRULI, which promotes translocation of YAP into the nucleus, promoting binding with TEAD transcription factors (, B F).', 'Notably, however, Hippo pathway modulation had minimal effect on proinflammatory responses of HS FBs, suggesting that fibrosis can be uncoupled from the inflammatory response in HS (G).', 'Modulation of the Hippo pathway in primary HS FBs.']","Figure 7 Modulation of the Hippo pathway in primary HS FBs. ( ) Illustration of Hippo pathway, created with BioRender (biorender.com). ( ) Quantitative PCR results showing the effect of TRULI or verteporfin (both 10 M) on ACTA2, COL1A1, and CTGF expression in HS FBs ( = 5; * < 0.05, **** < 0.0001; mean SD; ANOVA/Kruskal-Wallis test). ( ) Effect of TRULI or verteporfin (both 10 M) on smooth muscle actin (SMA) and collagen I levels in HS FBs by Western blotting ( = 5; * < 0.05; mean SD; Kruskal-Wallis test [collagen I], ANOVA [SMA]). ( ) Verteporfin blocked gel contraction in HS FBs. Data normalized to the corresponding NT (untreated) group ( = 5; * < 0.05; mean SD). ( ) TRULI significantly increased cell proliferation while verteporfin dose-dependently blocked cell growth among HS FBs ( = 3; * < 0.05, *** < 0.0001; mean SEM; 2-way repeated-measures ANOVA). The same NT group is shown in both panels. Cell proliferation was monitored by analysis of the area occupied by cells over time, using IncuCyte S3 Analysis software. ( ) Verteporfin showed a dose-dependent reduction in cell migration of HS FBs ( = 3; ** < 0.01, *** < 0.001; mean SEM; 2-way repeated-measures ANOVA). The same NT group is shown in both panels. ( ) Expression of cytokines and chemokines among untreated (NT), IL-1stimulated (10 ng/mL), and TNF-stimulated (10 ng/mL) primary HS FBs treated or not treated with TRULI or verteporfin ( = 5; * < 0.05, ** < 0.01, *** < 0.001; mean SD; 1-way repeated-measures ANOVA).",yes
PMC11392431,Figure_6,oa_package/09/54/PMC11392431.tar.gz,"['at the genus level (A).', 'In the BBR group, there was a considerable increase in the abundance of Verrucomicrobia, Akkermansia, and Akkermansia muciniphila (Akk bacteria) (s 6B,C).', 'The two groups significantly different microorganisms were compared, in turn (D).', 'Composition and difference analysis of microbiota in each group.']","Figure 6 Composition and difference analysis of microbiota in each group. Relative abundance composition at the phylum and genus levels in different groups. Results of LEfSe analysis (LDA >4, <0.05). Box comparison chart of significantly different bacteria (Top10).",yes
PMC6664842,Figure_5,oa_package/22/3d/PMC6664842.tar.gz,"['org/1999/xlink"" xlink:href=""10-1055-s-0039-1688105_00161_04""/>\nIncision over the tuberosity of navicular to expose the tibialis posterior tendon insertion.']",Fig. 5 Incision over the tuberosity of navicular to expose the tibialis posterior tendon insertion.,yes
PMC11572545,Figure_4,oa_package/94/7a/PMC11572545.tar.gz,"['Data clustering via rPhenograph from lungs of room air (N = 7) and combined DEP and malathion (N = 7) treated animals identified 23 unique myeloid and lymphoid populations (A).', 'When data is demarcated by treatment group, a clear separation between cellular subsets of the lung is present between controls (pink) and toxin exposed (green) animals (C).', 'Further characterization reveals a distinct shift between the most prominent cell subsets of the healthy lung (clusters 1 and 16) and those that become predominant following combined DEP and malathion exposure (clusters 18 and 20) (D).', 'Notably, cell subsets that are upregulated after toxin exposure (cluster 18 and 20) express significantly higher levels of Ly-6C and CD206 compared to subsets present during homeostasis (B).', 'Analyzing data generated from combined BAL samples from room air treated (N = 15) or combined DEP and malathion exposed (N = 12) animals, rPhenograph clustering resulted in 24 unique subsets (E).', 'When BAL clusters were grouped based upon treatment condition, unique cell subsets can be readily identified, although cell populations between treatment condition were far less homogenous then seen in the lung (G).', 'Here, we identified cell subsets that were unique to healthy animals (clusters 2 and 18), those that were conserved between experimental conditions (cluster 12 and 13), and a single, dominant population which arose following toxin exposure (cluster 1) (H).', 'Similar to cell changes found in the lung, the dominant cell subset present post-exposure was characterized by high expression of Ly-6C and CD206, as well as Siglec-F (F).', '.']","Fig. 4. High-dimensional immunophenotyping of lungs and BAL following aerosolized GWTs. Single cell suspensions of lung and BAL obtained from mice treated with either combination DEP and malathion (DEP + Mal) or room air (control) were evaluated by spectral cytometry and computational analysis. rPhenograph was used to generate tSNE plots (A, E) and associated heatmaps of marker expression per cell subset (B, F) from lung and BAL depicting 23 and 24 unique cellular subsets (clusters), respectively. Analysis of resultant tSNE from lung and BAL phenotyping by treatment illustrating subsets present in control (pink) or DEP + Mal (green) treated groups (C, G) with associated heatmaps depicting cluster expression per treatment group (D, H).",yes
PMC7815289,Figure_7,oa_package/47/7f/PMC7815289.tar.gz,"['Non-contrast chest CT, lung window, and axial planeNon-contrast chest CT, lung window, and axial plane show slight reduction in the extent of pneumomediastinum (green arrows) and subcutaneous emphysema (blue arrows).']","Figure 7 Non-contrast chest CT, lung window, and axial plane Non-contrast chest CT, lung window, and axial plane show slight reduction in the extent of pneumomediastinum (green arrows) and subcutaneous emphysema (blue arrows). Note bilateral subcutaneous tubes (purple asterisks).",yes
PMC11407578,Figure_3,oa_package/57/da/PMC11407578.tar.gz,"['ijd_346_23-t003"" ref-type=""table"">Table 3 and .', 'ijd_346_23-f003"">(a) Solitary scalp lesion in adult male; (b) Trichoscopic feature of AA on the scalp blue rectangle is empty follicular opening, the blue arrow is a yellow dot, a green circle is a peripilar sign and broken hairBeard lesions revealed the features: vascular network in 12 (92.']","Figure 3 (a) Solitary scalp lesion in adult male; (b) Trichoscopic feature of AA on the scalp blue rectangle is empty follicular opening, the blue arrow is a yellow dot, a green circle is a peripilar sign and broken hair",yes
PMC3529350,Figure_5,oa_package/fc/af/PMC3529350.tar.gz,"['Here, an immunohistochemical analysis was also performed for BSF that showed a strong signal in the Z- and H-bands of the IFM sarcomeres, but did not reveal a mitochondrial pattern (A,D G).', 'As similarly described for TBPH, the BSF distribution in sarcomeric bands was abolished upon CTG expression, whereas the BSF signal in the nucleus was strongly enhanced (B,D K), again suggesting a CTG-induced relocalization of BSF from cytoplasm to nucleus.', 'Finally, coexpression of i(CTG)480 with human MBNL1 [Mhc-Gal4 UAS-i(CTG)480 UAS-MBNL1] also rescued the normal distribution of BSF in sarcomeric bands, although the nuclear signal was again more intense than in the controls (C).', '.']","Fig. 5. (AC) Fluorescent confocal images of rostrocaudal cryosections from adult thoraces stained with an anti-BSF antibody (green), and counterstained with phalloidin (red). In control flies (Mhc> ; A) BSF was detected preferentially in the cytoplasm as a constituent part of the sarcomeric bands (see also ). (B) Expression of i(CTG)480 in the muscle [ ] disrupted cytoplasmic BSF signal. (C) Coexpression of i(CTG)480 with human MBNL1 [ ] partially rescued the sarcomeric localization of BSF. (DK) Fluorescent confocal images comparing the subcellular distribution of i(CUG)480 RNA (fluorescent in situ hybridization using a CAG red-labeled probe; D,H) with BSF protein (green; F,J; see merge in G,K) in control (DG) and CTG-expressing flies (HK). Expression of CTG repeats in the muscle not only abolished cytoplasmic BSF signal, but also enhanced its detection in the nuclei. Nuclear BSF signal did not seem to colocalize with CUG-RNA foci. Nuclei were counterstained with DAPI (blue; E,I).",yes
PMC7592685,Figure_1,oa_package/4a/87/PMC7592685.tar.gz,['Comparison between measured values and diagnosis.'],"Fig.1 Comparison between measured values and diagnosis. For each biomarker, the measured value was plotted against the diagnosis. NC (negative control, blue); MCI (mild cognitive impairment, green); AD (Alzheimer disease, red). The -values between each diagnosis are calculated by test. When the -values are larger than 0.05, they are shown as n.s.",yes
PMC11244770,Figure_1,oa_package/86/d7/PMC11244770.tar.gz,"['t004"" ref-type=""table"">4 are shown as .', 'g001Icariin improves weight and renal function in the nephrotic syndrome rat induced by doxorubicin.']",10.1371/journal.pone.0298353.g001,yes
PMC10525886,Figure_5,oa_package/e5/57/PMC10525886.tar.gz,"['A neointimal membrane formed on both the inner and outer surfaces of the e-PTFE, covering the 3D balloon spacer, with a more significant thickening observed on the outer surface ().', 'Examination of harvested samples for tricuspid valve and Pivot Mandu device after implantation into pigs: (a) after 8 weeks (confirmed tissue covering); (b) after 24 weeks; (c) pathology of septal leaflet after 24 weeks; (d) pathology of e-PTFE material on 3D balloon spacer, which comes into contact with the tricuspid valve, after 24 weeks (confirmed formation of endothelium).']","Figure 5 Examination of harvested samples for tricuspid valve and Pivot Mandu device after implantation into pigs: ( ) after 8 weeks (confirmed tissue covering); ( ) after 24 weeks; ( ) pathology of septal leaflet after 24 weeks; ( ) pathology of e-PTFE material on 3D balloon spacer, which comes into contact with the tricuspid valve, after 24 weeks (confirmed formation of endothelium).",yes
PMC4532934,Figure_1,oa_package/fc/c4/PMC4532934.tar.gz,"['Contrast enhanced CT of abdomen revealed the typical target sign in the right lower abdomen suggestive of intussusception with an intraluminal homogenous hypodense lesion at the apex of intussusception most likely suggestive of lipoma ().', '0-1344430929415674501CECT showing typical target sign.']",Figure 1 CECT showing typical target sign.,yes
PMC9626037,Figure_4,oa_package/bc/e3/PMC9626037.tar.gz,"['Chest radiograph (CXR) demonstrated bilateral multifocal airspace opacities with prominent interstitial markings (\nA\n).', 'Given her acute respiratory decompensation with concern for PE, the patient underwent CTA chest, which demonstrated PAU at the distal aortic arch (\nB\n).', '\n(\nA\n) Admission anteroposterior chest radiograph of patient 5 demonstrating bilateral multifocal patchy airspace opacities and prominent interstitial markings.', 'As part of his preoperative evaluation for transcatheter aortic valve replacement (TAVR), he underwent CTA chest, abdomen, and pelvis, which revealed a linear flap in the proximal aortic arch suspicious for a previous penetrating ulcer (\nC\n).']",Fig. 4 ( ) Admission anteroposterior chest radiograph of patient 5 demonstrating bilateral multifocal patchy airspace opacities and prominent interstitial markings. ( ) Computed tomography angiogram (CTA; coronal view) of patient 5 demonstrating 1.5cm0.8cm outpouching at descending aortic arch compatible with penetrating ulcer (arrow). ( ) CTA (sagittal view) of patient 6 demonstrating noncalcified ulcerated plaque in proximal aortic arch without intramural hematoma (arrow).,yes
PMC10297602,Figure_24,oa_package/54/6e/PMC10297602.tar.gz,[],"Figure 24 Sagittal STIR ( ), T1 ( ), axial STIR ( ) and T1 ( ) images of a sacral giant cell tumour (white arrows). There is intermediate to low signal intensity on both T1 and T2.",yes
PMC4231236,Figure_4,oa_package/17/c0/PMC4231236.tar.gz,"['PRL-3 overexpression counteracted the drug sensitivity of AML cells to Ara-C, but the PRL-3 mutant (C104S) did not (A,B).', 'In addition, knockdown of the endogenous PRL-3 obviously sensitized cells to the cytotoxicity of the drug (C).', '05, D,E).', '05, F).', 'Upon Ara-C treatment, the apoptotic proteins PARP, caspase-9, and caspase-3 were clearly activated in ML-1 cells where the expression of endogenous PRL-3 was silenced by siRNA (G).', 'Our immunoblotting results showed that PRL-3 can effectively enhance STAT5 phosphorylation (H).', 'In line with our observation in other solid tumor cells,22 PRL-3 overexpression could activate AKT to some extent, rather than the further downstream effectors, including 4E-BP1 and p70S6K in both ML-1 and U937 cells (H).', 'Results presented in this study show that PRL-3 overexpression can activate STAT5 (H) in FLT3-ITD negative AML cells, which may in turn upregulate PRL-3 expression, as reported.']","Figure 4 The effect of PRL-3 on drug sensitivity and drug-induced apoptosis. (A-C) Cytotoxicity analysis of U937 (A) and ML-1 (B) cells with ectopic expression of PRL-3 or its mutant, or with silenced endogenous PRL-3 by the specific shRNAs as shown (C). The cells were treated with Ara-C or doxorubicin (DNR) in the indicated concentrations for 72 hours, and assayed by CCK8 methods. (D-F) Annexin V/7-AAD staining and flow cytometry analysis of U937 (D) and ML-1 (E,F) in the indicated conditions. Cells were treated with 0.4 M Ara-C or 0.04 M DNR for 24 hours and analyzed with flow cytomery. The representive histograms and statistical analysis are indicated. Data were shown as meanSD of triplicate experiments. * <.05; ** <.01, n=3. (G) Western blots of the apoptosis-related proteins as indicated in ML-1 whole-cell lysates. The indicated cells were treated with 0.4 M Ara-C for 24 hours and lyzed for immunoblotting. (H) Western blots of the survival-related proteins as indicated in ML-1 and U937 whole-cell lysates.",yes
PMC11353895,Figure_8,oa_package/a2/15/PMC11353895.tar.gz,"['The clinical CXRs were used as input images, and their corresponding bone extraction images are depicted in .', 'Samples of bone extraction results (b) of trained U-Net from clinical CXRs (a).']",Figure 8 Samples of bone extraction results ( ) of trained U-Net from clinical CXRs ( ).,yes
PMC4254201,Figure_2,oa_package/48/de/PMC4254201.tar.gz,"['Of randomly selected 4 benign bladder diverticula and 8 radical cystectomy Specimens without diverticulum ( 2), hypertrophic muscularis mucosae was identified in one out of 4 benign bladder diverticula and 2 out of 8 radical cystectomy specimens treated for conventional (not associated with diverticulum) urinary bladder carcinoma.', '\nRepresentative sections of hypertrophic muscularis mucosae.', 'Clinical follow-upClinical follow-up was available for all 22 patients, with a mean follow-up of 38 months (range 1 135 months, median 32 months).']","Figure 2 The H&E sections from bladder wall of a benign bladder diverticulum with completely denuded urothelium (upper tissue edge), showing hypertrophic muscularis mucosae . and are from the conventional (lesion not arising from diverticula) neoplastic bladder wall of a well-differentiated squamous cell carcinoma, showing hypertrophic muscularis mucosae. Low ( , 40X) or higher magnifications ( , 100X), respectively.",yes
PMC6826277,Figure_1,oa_package/72/a5/PMC6826277.tar.gz,"['jpg""/>INTRODUCTIONThe cell and carcinogenic transformationThe cells in a multicellular organism possess a massive number of systems to ensure not only the survival of individual cells but also the organism as a whole [].', ':Simplified illustration of a cell, pointing out some of the important mechanisms of viral oncomodulation.', ') from the mitochondria [].', '[82] STAT3 plays an important role in tumor proliferation, invasion, angiogenesis, and immune suppression, primarily through stimulation of inflammatory pathways such as NF B and IL-6 [].', '[57,229]High levels of IL-6 have been associated with a poor prognosis in several types of cancer [].']","Figure 1: Simplified illustration of a cell, pointing out some of the important mechanisms of viral oncomodulation.",yes
PMC7372300,Figure_3,oa_package/a1/6a/PMC7372300.tar.gz,['Example of a thymic carcinoma fixed in formalin at 4 C and embedded in paraffin.'],"Figure 3 Example of a thymic carcinoma fixed in formalin at 4C and embedded in paraffin. The slides were scanned with the Aperio system 40. Good morphological details are observed. Hematoxylineosin (HE) stain, 200, showing the atypical epithelial cells forming ribbons infiltrating sclerotic tissue. CD117 stain, 200. Most cells are stained with this thymic carcinoma marker. Glut-1 stains in thymic carcinoma ribbons and networks of epithelial cells.",yes
PMC10608316,Figure_9,oa_package/15/bc/PMC10608316.tar.gz,"['CT is the most sensitive technique for detecting intraperitoneal fatty nodules, which are commonly observed around the liver surface () [64,65].', 'Ruptured ovarian teratoma in a thirty-eight-year-old woman presenting with pelvic pain and fever.']","Figure 9 Ruptured ovarian teratoma in a thirty-eight-year-old woman presenting with pelvic pain and fever. CT axial ( , ), coronal ( ), and sagittal ( ) planes reveal a sizeable pelvic mass (yellow arrows) composed of fat and fluid with discontinued walls (red arrow) and an interior tooth-like calcification (green arrowheads). Subphrenic fatty implants (blue arrows) are also observed ( , ). After adnexectomy, histology confirmed the diagnosis of right mature teratoma.",yes
PMC9627685,Figure_2,oa_package/cb/35/PMC9627685.tar.gz,['.'],Figure 2. Axial image of abdominal CT scan in lung window demonstrating air in the bowel wall loculated around an intussuscept-like swirl of bowel loops (black arrow).,yes
PMC5106215,Figure_5,oa_package/3d/08/PMC5106215.tar.gz,"['From the PLO assays using total lipid extracts, we found that the amount of TAPP1-PH-interacting PtdIns(3,4)P2 was increased in SH-SY5Y cells following exposure to A 1-42 ( figure supplement 1A).', 'In contrast, there was no such increase by A 1-42 in SH-SY5Y/FCGR2B or SH-SY5Y/INPPL1 knockdown cells ( figure supplement 1B).', ', 2007), was reduced by A 1-42 in SH-SY5Y cells, but not in SH-SY5Y/FCGR2B or SH-SY5Y/INPPL1 knockdown cells ( figure supplement 1B).', 'Similar patterns of changes in the levels of TAPP1-PH-interacting or GRP1-PH-interacting lipids were also observed in WT primary cortical neurons after treatment with A 1-42 (A,B).', 'When we directly measured the levels of phosphoinositides with an ELISA assay, we observed that the levels of PtdIns(3,4)P2 were increased by 30% in primary cortical neurons after exposure to A 1-42, whereas PtdIns(3,4,5)P3 was decreased by 17% (C).', ', 2008), and also in FcgR2b KO neurons (C).', 'While TAPP1-PH-GFP was found in a diffuse pattern in the cytosol of untreated SH-SY5Y cells, treatment with A 1-42 enhanced the fluorescence of TAPP1-PH-GFP and concentrated it at the plasma membrane (D).', 'In contrast, A -induced accumulation of TAPP1-PH-GFP at the plasma membrane was impaired in SH-SY5Y/FCGR2B knockdown cells (D).', 'However, this localization was not affected by Fcgr2b deficiency ( figure supplement 1C).', '10.', '014.', '01410.', '015 figure supplement 1.', '01510.', '016 figure supplement 2.', '016To address the important question of whether the increase in PtdIns(3,4)P2 by A 1-42 can influence tau phosphorylation, we directly delivered phosphoinositide into living neurons using a carrier (Ozaki et al.', 'Compared to untreated control cells, treatment with PtdIns(3,4)P2 increased tau phosphorylation (AT180, CP13, PHF1) in primary cortical neurons in a dose-dependent manner (E).', 'Consistent with the activation of GSK3 by A 1-42, PtdIns(3,4)P2 treatment also reduced the inhibitory phosphorylation of GSK3 at Ser9 in neurons ( figure supplement 2A).', 'Moreover, PtdIns(3,4)P2 induced the expression of GRP78, a typical marker of unfolded protein response (UPR), as well ( figure supplement 2A).', 'We further found that PtdIns(3,4)P2-induced GSK3 activation and tau phosphorylation were attenuated by the treatment with ER stress inhibitors, such as 4-PBA and Salubrinal, a chemical chaperone and an eIF2 dephosphorylation inhibitor, respectively ( figure supplement 2B).', 'We confirmed that ER stress response and GSK3 activation triggered by A were all declined by a SHIP2 inhibitor AS1949490 or lentiviral expression of Inppl1 siRNA ( figure supplement 2C,D).', 'We also found that PtdIns(3,4)P2 abolished the inhibitory phosphorylation of GSK3 at Ser9 (GSK3 activation) and increased tau hyperphosphorylation ( figure supplement 2A).', 'In addition, we found that treatment with ER stress inhibitors, such as 4-PBA and Salubrinal, a chemical chaperone and an eIF2 dephosphorylation inhibitor, respectively, apparently attenuated PtdIns(3,4)P2-induced GSK3 activation and tau phosphorylation with some different efficacy ( figure supplement 2B).', 'Further, treatment with AS1949490, a SHIP2 inhibitor, or infection with lentivirus carrying Ship2 shRNA blocked A -induced GSK3 activation and tau hyperphosphorylation ( figure supplement 2C, D), again confirming our proposal that activation of SHIP2 elicits ER stress to activate GSK3 and subsequent tau hyperphosphorylation.', 'We included these results into supplementary information ( figure supplement 2) (subsection Dysregulation of phosphoinositide metabolism by the Fc RIIb-SHIP2 axis for tau 269 phosphorylation , last paragraph).', '026Finally, consistent to our proposal, there are references showing the expression of Fc RIIb mRNA and proteins in neurons:(1) Cahoy et al.']",10.7554/eLife.18691.007,yes
PMC9674403,Figure_2,oa_package/a7/7e/PMC9674403.tar.gz,['Axial T2-weighted image reveals retention cyst in the right maxillary sinus (arrow).'],Figure 2 Axial T2-weighted image reveals retention cyst in the right maxillary sinus (arrow).,yes
PMC10577113,Figure_6,oa_package/8f/83/PMC10577113.tar.gz,"[' 6a).', ' 6b, this treatment not only did not improve the cognitive decline observed in APP/PS1 at this age, but it also worsened the performance of both strains, WT and APP/PS1 in the acquisition phase of the MWM.', ' 6c).', ' 6d) nor neuroinflammatory cytokines levels (', ' 6e) and insulin (', ' 6f) tolerance tests were performed, at the periphery AGK-2 treatment increased the expression of IL-1B (', ' 6g, h), Tnf- (', ' 6i), Tgf- (', ' 6j), IL-6 (', ' 6k), MCP-1 (', ' 6l), and TNF (', ' 6m).', 'Peripheral SIRT2 inhibition impairs memory and increases systemic inflammation.', 'Results are shown as mean SEMSIRT2 Is Upregulated in Postmortem Cerebral Cortex Samples from AD PatientsGiven the different roles that SIRT2 seems to be playing at central and peripheral levels, we finally analyzed its expression in brain and plasma samples from AD patients.']","Fig. 6 Peripheral SIRT2 inhibition impairs memory and increases systemic inflammation. ( ) Habituation phase of the MWM. ( ) Escape latency in the acquisition phase of the MWM and corresponding area under the curve (AUC) of the acquisition curve (F=6.716, *p<0.05 main effect of treatment; F=6.580, #p<0.05 main effect of genotype, two-way ANOVA, n=68 animals per group). Note that AGK-2 treatment worsened learning capacities in both WT and APP/PS1 mice. ( ) Representation of the percentage of time spent in the correct quadrant in the retention phase of the MWM (5 Day: F=4.474 *p<0.05, main effect of treatment; 8 Day: F=4.854, #p<0.05, main effect of genotype, two-way ANOVA). ( ) Representative hippocampal sections of -amyloid plaques stained with 6E10 antibody in brain slices (left) and amyloid burden quantification (right) in 8 months-old APP/PS1 mice treated for two months with vehicle or AGK-2 (n=3 animals per group, 2 sections including hippocampus and frontal cortex per animal) Scale bar=500 m. Glucose ( ) and Insulin ( ) tolerance tests. No significant differences were observed between vehicle or AGK-2 treated animals (n=59 mice per group). Peripheral protein expression of ( ) IL-1 (F=5.951, *p<0.05, main effect of treatment, two-way ANOVA) and gene expression of ( ) (F=16.33, ***p<0.001, main effect of treatment, two-way ANOVA), ( ) (F=19.60, ***p<0.001, main effect of treatment, two-way ANOVA) and ( ) (F=11.49, **p<0.01, main effect of treatment, two-way ANOVA) (n=6 animals per group). was used as an internal control. Serum levels of the cytokines ( ) IL-6 (F=10.80, ***p<0.001, main effect of treatment, two-way ANOVA), ( ) MCP-1 and ( ) TNF (F=5.926, *p<0.05, main effect of treatment, two-way ANOVA) (n=58 mice per group). Results are shown as meanSEM",yes
PMC10485621,Figure_6,oa_package/46/94/PMC10485621.tar.gz,"['At 4 weeks we find no significant difference in the mean striation continuity of the entire fiber between mdx and WT (G), the 4-week mdx do achieve lower minimum continuity scores than the 4-week wild-type.', 'While we found an increase in the average continuity score in wild-type, the continuity of the mdx decreased as altered morphologies emerge at a greater frequency (G).']","FIGURE 6 Median Striation Continuity scores decrease with altered morphology. Striation detection for the full 16weeks wild-type fiber depicted in , the 16weeks mdx fiber with braided myofibrils, and the 16weeks mdx fiber with wrapped myofibrils. Their respective continuity scores per z-slice are illustrated with min, max, median scores displayed in boxplots . Average striation continuity scores for each fiber within each experimental group ( = 50125 fibers per condition, across 5 mice). One-way ANOVA revealed significant difference between groups with dks multiple comparisons test elucidating a significant difference between 16weeks WT and MDX (5 mice per age/genotype) ( (3, 333) = 38.36, < 0.0001).",yes
PMC8942730,Figure_16,oa_package/6f/d0/PMC8942730.tar.gz,[],"Fig. 16. Hepatic abscesses. A, B. A 59-year-old man presented with fever, right upper quadrant pain, abnormal liver function tests, and leukocytosis. Grayscale (A) and color Doppler (B) ultrasonography show a hypoechoic lesion (arrows) with heterogeneous internal echoes in the liver without internal vascularity, suggestive of a liquified abscess. C, D. A 60-year-old man presented with right upper quadrant pain, abnormal liver function tests, and leukocytosis. Grayscale (C) and color Doppler (D) ultrasonography show a complex solid and cystic mass (dashed arrows) in the liver parenchyma with mild internal vascularity suggestive of a partially liquified abscess.",yes
PMC8415271,Figure_3,oa_package/e9/4f/PMC8415271.tar.gz,"['9% NaCl saline, respectively; (25)] or vehicle alone was injected subcutaneously (10 L/g body mass) for 5 days preceding, then throughout reversal trials ().', '(A) Behavioral timeline schematic for the two-choice Odor Discrimination Flexibility (ODF) task.', 'Odor Discrimination Flexibility TaskBehavioral AssayMice were habituated to reinforcers (sweetened yogurt chips; Bio-Serv, Flemington, NJ, USA) and the test environment (3 min/day) for 7 and 5 days, respectively, preceding shaping trials (A).', 'Mice were trained 5 days per week in the two-choice operant paradigm wherein one olfactory stimulus was paired with the reinforcer (A).', 'Shaping trials were continued during drug habituation to maintain learned responses, and reversal training began on injection day 6 (A).', 'Shaping acquisition was significantly faster in Tat(+) relative to Tat( ) subjects [B; X(1,N=23)2 = 6.', ""Specifically, within Tat(+) subjects, MJN110 treatment significantly increased the number of trials required to acquire the reversal learning task (B'; 22.""]",Figure 3 Behavioral timeline schematic for the two-choice Odor Discrimination Flexibility (ODF) task. Tat(+) subjects acquired the shaping task significantly faster than Tat() controls. MJN110-treated Tat(+) subjects acquired the reversal task significantly slower than vehicle-treated Tat(+) subjects. Data are mean SEM. Statistical significance was determined using ANOVA and Bonferroni correction where applicable. An alpha level of < 0.05 was considered significant for all statistical tests. * < 0.05 vs. Tat(); < 0.05 vs. Tat(+)/vehicle.,yes
PMC7498635,Figure_3,oa_package/bb/72/PMC7498635.tar.gz,"['Manual digital subtraction angiography series were performed, confirming a total occlusion of the SVC beginning at the right atrium 5 cm in length and retrograde flow via the enlarged azygos vein ( 3A; Video 1).', 'The occlusion was recanalized by incremental balloon angioplasty with 10 60 mm, 16 40 mm, and 18 40 mm balloons ( 3B; Video 2).', 'Owing to significant elastic recoil ( 3C, Video 3), a self-expanding 20 60 mm uncovered stent (Optimed Sinus XL; Optimed, Esslingen, Germany) was placed into the SVC, followed by dilatation with an 18 40 mm balloon.', 'Complete cessation of collateral blood flow via the azygos vein was confirmed ( 3D, Video 4).', ' 3Digital subtraction angiography (DSA), balloon angioplasty, and stenting of the superior vena cava (SVC).', 'Supplemental 2BChest-x-ray in lateral projection at 6 month-follow up.']","Figure3 Digital subtraction angiography (DSA), balloon angioplasty, and stenting of the superior vena cava (SVC). DSA showing complete occlusion of the SVC and retrograde flow via the azygos vein. Balloon angioplasty of the chronic occlusion. DSA after incremental balloon angioplasty of the SVC showing significant elastic recoil. DSA after stent deployment confirming optimal angiographic result and complete cessation of collateral flow via the vena azygos.",yes
PMC10475251,Figure_1,oa_package/fb/83/PMC10475251.tar.gz,"['On physical examination, there was a 5-cm nodular and ulcerated mass with a wide implantation base, located in the left frontotemporal region ().', '.', '1177_2050313X231197325-fig1"" position=""float""/>A biopsy specimen was sent for pathological examination.']",Figure 1. Clinical appearance of the tumour.,yes
PMC9301314,Figure_7,oa_package/16/93/PMC9301314.tar.gz,"['We found an inverse relationship between the miR-183C miRNAs expression and Foxo1 protein expression in the splenocytes of MRL and MRL/lpr mice (A).', 'Knocking down miR-182 in vitro alone with antagomir-182 or miR-183C cluster with the mixes of antagomir-182, -96, -183 in MRL/lpr splenocytes increased Foxo1 protein expression (B).', 'Further, Foxo1 protein expression was significantly increased in CD4+ T cells from miR-182 / B6/lpr and miR-183 / B6/lpr compared to the CD4+ T cells from control mice (C).', 'Deletion of miR-182 or miR-183C increased Foxo1 expression (C) and reduced IFN in B6/lpr splenocytes in response to anti-CD3 plus anti-CD28 or PMA plus ionomycin stimulation (s 6A,D).', 'We confirmed the significant reduction of Foxo1 mRNA expression level in the Foxo1 siRNA transfected cells (D).']","FIGURE 7 miR-183C and miR-182 miRNAs regulate IFN production via targeting Foxo1. An inverse relationship between miR-183 miRNAs expression of Foxo1 protein expression in control MRL and autoimmune-prone MRL/lpr mice splenocytes. The left graph showed relative miR-183, -96, -182 miRNAs expression in the splenocytes of MRL and MRL/lpr mice (1415weeks of age). The graph showed means SD ( = 3 each). Unpaired student t -test (MRL vs. MRL/lpr); *, < 0.05; **, < 0.01. The right panel showed western blotting of Foxo1 and -actin (loading control) protein expression in MRL and MRL/lpr splenocytes. The representative western blotting image from three independent experiment was shown. Increased Foxo1 protein in antagomir-182 or antagomir-183C treated splenocytes from MRL/lpr mice. The representative western blotting image from two independent experiments was shown. Increased Foxo1 protein expression in splenic CD4 T cells from miR-183C B6/lpr (miR-183C ), miR-182 B6/lpr (miR-182 ) mice when compared to the cells from their respective control miR-183C B6/lpr (miR-183C ) and miR-182 B6/lpr (miR-182 ) mice. The representative western blotting image from at least three independent experiments was shown. Reduced Foxo1 mRNA expression in Foxo1 siRNA treated cells when compared to negative control siRNA (NC siRNA) treated cells. The graph showed means SD ( = 2 each). Inhibition of Foxo1 in splenocytes of miR-183 and miR-182 mice increased IFN, but not IL-6 production in response to anti-CD3/anti-CD28 or PMA/ionomycin stimulation. The cytokine level in Foxo1 siRNA treated samples were shown as the percentage of paired NC siRNA treated cells. The graphs were shown as means SD ( 4). Paired student t tests were performed (NC siRNA vs. Foxo1 siRNA); *, < 0.05 and **, < 0.01.",yes
PMC4277831,Figure_3,oa_package/e0/4c/PMC4277831.tar.gz,"['Western blots analysis of Triton and SDS extract using anti-tau antibodies showed that A 1-42 treatment resulted in increased phosphorylation at Ser262, as recognized by the pS262 antibody, in both Triton and SDS soluble fractions as compared with N2a cells that did not receive A treatment ( 3A and C).', 'In A 1-42 treated cells we also observed a 3-fold increase in total tau and phosphorylation at the PHF-1 epitope (Ser396/Ser404) in the SDS fraction, but we observed no change in A 1-40 treated cells ( 3B and D).', 'We also observed an increase in Tau421 (cleavage of tau at Asp421) in the Triton fraction after A 1-42 treatment as visualized with the tau-C3 antibody ( 3A and B).', '\nA\n 1-42 promotes phosphorylation, cleavage and aggregation of tau by increasing GSK3 \nactivity and by activating pro-caspase3.', 'Activation of GSK3 and caspase-3 by A 1-42 but not by A 1-40GSK3 phosphorylates tau at many sites including Ser262, Ser396 and Ser404.', 'As shown in E, A 1-42 treatment increased p-GSK3 (Y216) without changing the total GSK3 level.', 'Western blots with an anti-active caspase-3 antibody revealed higher levels of active caspase-3 following A 1-42 treatment but not following A 1-40 treatment ( 3E and F).', 'We found that A 1-42 activated GSK3 , but A 1-40 did not, which may explain the different effects of A 1-40 and A 1-42 on tau phosphorylation shown in 3A and C.', 'This may explain the marked difference we observed between the level of Tau421 in A 1-42 and A 1-40 treated cells ( 3).']","Figure 3 Sequential extraction was performed on Tau441-YFP-transfected cells treated with transfection reagent alone (control), 200 nM A1-40 or A1-42 for 24h. Triton fraction was obtained by extracting intracellular tau with 1% Triton. Triton-insoluble fraction was then solubilized with 1% SDS to get SDS fraction. Triton fraction was electrophoresed and immunoblotted for total tau (HT7), p-tau(Ser ) (PHF-1), p-tau(Thr ) (pS262), Tau421(tau-C3) and -actin. -Actin was measured from the same blots to ensure that equal protein was present in every lane. A1-42 significantly induced p-tau at Thr and increased levels of Tau421 were observed in A1-42 treated cells, while no change occurred in A1-40 treated cells when compared to cells without treatment. Quantification of western blots in . Representative images of western blots for SDS fraction with HT7, PHF-1 and pS262. All 3 forms of tau increased in A1-42 treated cells. No change in tau level was found in A1-40 treated cells. There was no detectable signal for tau-C3 in SDS fraction. Quantification of western blots in . Triton fraction was also blotted with anti-GSK3 antibody, anti-p-GSK3 (Y216) and anti-caspase-3 antibody. A1-42 treatment resulted in an increase in phosphorylation of GSK3, which represents higher GSK3 activity. More active caspase-3 was detected in A1-42 treated cells. Quantification of western blots in . Histographs show mean +/ standard error, n =3. *p <0.05; ** p <0.01; ***p <0.005.",yes
PMC10400043,Figure_3,oa_package/8e/82/PMC10400043.tar.gz,"['Well-to-moderately differentiated cases appear as nests of pyramidal-shaped cells that cluster around a lumen, the so-called acinar pattern[115] [].', 'Histologic appearance of PACC.']","Figure 3 Histologic appearance of PACC. H&E specimens of PACC. A) Low power image showing how PACC recapitulates the architecture of the exocrine pancreatic parenchyma with back-to-back acinar structures containing lumina. The tumor clusters are irregular in size and shape and are approaching the peri-pancreatic adipose tissue (40x). B) Higher power image showing the classic cytologic features of PACC with basally located nuclei containing single prominent nucleoli, and abundant apical amphophilic and granular cytoplasm (400x).",yes
PMC8656278,Figure_7,oa_package/1c/93/PMC8656278.tar.gz,[],"Figure7 Gross morphology and histological images of viable tumor. The areas represented from periphery to center are as follows: viable tumors (VT); reaction zone (RZ); ablation region (AR) Gross morphology image of viable tumor ( , yellow arrow) was appeared as nodular located on the periphery of the RFA region. 40 H&E histology showed VT can infiltrate and compress the fibrous rim, causing local RZ thinner than the adjacent tumor-free area. showed 400 H&E histology of VT , Inj , AR and FR from . showed typical clumps of heteromorphic tumor cells with large, hyperchromatic nuclei.",yes
PMC7576851,Figure_4,oa_package/0c/ba/PMC7576851.tar.gz,"[' 4).', '', '05Of the seven MOIs with an increase in the Iba1low population in AD, the mean population intensity of CD45, HLA-DR, CD14, CD74, and CD32 was significantly higher in the Iba1low MOIhigh population relative to the Iba1high MOIhigh population in both normal and AD, suggesting that cells with the highest MOI expression were those with low Iba1 (', ' 4A D, G).', ' 4F).', ' 4I).', ' 4J).']","Fig.4 Iba1 MOI population shows higher expression of activation markers than the respective Iba1 MOI populations. Iba1-MOI populations were identified by immunofluorescent co-labelling of Iba1 with markers CD45 ( ), HLA-DR ( ), CD14 ( ), CD74 ( ), CD33 ( ), CD206 ( ), CD32 ( ), CD163 ( ), P2RY12 ( ), and TMEM119 ( ). The mean population intensity of the markers was measured for the Iba1 MOI and Iba1 MOI populations in each normal and AD case. The mean population intensities of each MOI were compared between the Iba1-MOI populations, and normal and AD using a two-way ANOVA with Tukeys multiple comparisons test. Data are presented as meanSD (n=7-8). Significance of differences between Iba1-MOI populations, and normal and AD: **** 0.0001, *** 0.001, ** 0.01, * 0.05",yes
PMC7440889,Figure_7,oa_package/ef/74/PMC7440889.tar.gz,"['Our results show that there were significantly lower levels of IL-1 (A and E; P = .', '006, n = 6, IL-18 (B and F; P = .', '01, n = 6), IL-6 (C and G; P = .', '015, n = 6), and increased BDNF (D and H; P = .', '007, and I and J; P = .', '.', '1177_2633105520942480-fig7""/>.', 'The results found that the deletion of this gene was associated with increased BDNF levels and protected the mice from neuroinflammation (A to J).']","Figure 7. Deletion of NLRP3 is associated with decreased neuroinflammation and lower BDNF levels. (A to D) IL-1, IL-18, IL-6, and BDNF immunoreactivity in the frontal cortex. Representative immunohistochemistry micrographs showing IL-1, IL-18, IL-6, and BDNF, respectively, in frontal cortex tissues of GW chemical treated (GWP), GW chemical treated NLRP3KO (GWP-NLR3KO), and vehicle control (CONT) treated mice (magnification 40 and scale bar 50m). (E to H) Morphometric analysis (represented as % ROI) obtained from 10 to 15 images from different microscopy fields from each mouse sample. Data are represented as meanSEM. (* <.05, n=6). (I) Western blots of BDNF protein levels in the frontal cortex of GWP and CONT treated mice. (J) Morphometry analysis of all immunoblots normalized against -actin. Data are represented as meanSEM (* =.05, n=5). BDNF indicates brain-derived neurotrophic factor; GW, Gulf War; SEM, standard error of the mean.",yes
PMC6798051,Figure_3,oa_package/03/ed/PMC6798051.tar.gz,"['001; k = 21, A).', '05) during encoding and retrieval by older dropouts (B).', 'Prefrontal hyper-activation.']","Figure 3 Prefrontal hyper-activation. Significant interaction effect suggesting group-by-condition differences in the recruitment of ventrolateral prefrontal cortex (VLPFC) at baseline. analyses revealed that the VLPFC was differentially recruited during retrieval for older remainers but recruited to a similar degree during encoding and retrieval for older dropouts (bars derived from the interaction peak voxel, enc, encoding; ctr, control task).",yes
PMC6930130,Figure_4,oa_package/7e/f7/PMC6930130.tar.gz,"['\nMedial approach through less than 1 cm skin incision was performed, performed at the level of the neck of the first metatarsal (\n\n).', '\nMedial approach through less than 1-cm skin incision performed at the level of the neck of the I\nst\nmetatarsal bone.']",Fig. 4 Medial approach through less than 1-cm skin incision performed at the level of the neck of the I metatarsal bone.,yes
PMC4506845,Figure_1,oa_package/dc/08/PMC4506845.tar.gz,"['Before we understand the development of the fovea in infants, we must be familiar with some definitions used to describe the layers and zones ():Central foveal thickness (CFT, yellow line) which is the thickness of the entire retina from the inner aspect of the inner limiting membrane (ILM) to the inner aspect of the retinal pigment epithelium (RPE) at the foveal center.', 'In pressThe zones and the layers marked on the SD OCT image of a neonate.']",Figure 1 The zones and the layers marked on the SD OCT image of a neonate.,yes
PMC3197137,Figure_5,oa_package/85/fe/PMC3197137.tar.gz,"['The overall differences in shape between the control hippocampi, those affected by AD, and those affected by sclerosis are visible in .', ""g005The average shapes of hippocampi affected by neither Alzheimer's disease (AD) or hippocampal sclerosis (HS) (N = 47), AD only (N = 40), and HS (N = 13, 9 with AD and 4 with HS only), viewed from the superior direction.""]",10.1371/journal.pone.0026286.g005,yes
PMC8361203,Figure_1,oa_package/14/7b/PMC8361203.tar.gz,"[' 1.', ' 1) would alleviate the need to wait for the special stains to be available.', 'Overview of deep learning-based H E stain transformation into special stains.', 'ResultsIn order to prove the utility of our stain transformation technique, we investigated whether it can be used to improve preliminary diagnoses made by pathologists when only H E is available.']",Fig. 1 Overview of deep learning-based H&E stain transformation into special stains. Histochemical staining of H&E is digitally transformed using a deep neural network into the special stains: (i) generation of JMS (purple arrow); (ii) generation of MT (red arrow); (iii) generation of PAS (blue arrow).,yes
PMC4182477,Figure_6,oa_package/a0/77/PMC4182477.tar.gz,"['The morphology of cell was not significantly altered in the hippocampus of GIGYF2-knockdown diabetic mice (A).', '05, B and C), but had no significant difference compared to the control group (p = 0.', 'g006Haematoxylin and eosin staining (HE) of hippocampal tissue.', 'Moreover, our results showed that down-regulation of GIGYF2 could reduce changes of cell morphology such as neuronal apoptosis and disordered arrangement (A).', 'Our current results showed that the over-expression GIGYF2 is correlated with an increase neuron apoptosis ( and 7).']",10.1371/journal.pone.0108559.g006,yes
PMC8710864,Figure_1,oa_package/f5/98/PMC8710864.tar.gz,['Middle-aged patient with a history of liver cirrhosis presented with haematemesis.'],"Figure 1 Middle-aged patient with a history of liver cirrhosis presented with haematemesis. Oesophagogastroduodenoscopy demonstrated gastric varices in the setting of portal hypertension. For TIPS creation, access was obtained into the portal venous system using ICE catheter guidance. (A) The TIPS needle is seen within the middle hepatic vein, with direct visualisation of the portal venous system as the target. (B) The TIPS needle is visualised advancing through the hepatic parenchyma towars the proximal left portal vein. (C) The TIPS needle is shown within the hepatic parenchymal tract reaching the proximal left portal vein. (D) The ICE catheter demonstrates the wire and catheter within the main portal vein. (E) On digital subtraction angiography, the access for TIPS creation is depicted between the middle hepatic vein and the most proximal portion of the left portal vein, with a 5 French flush pigtail catheter formed within the main portal vein. Of note, the ICE catheter tip is faintly seen within the inferior vena cava, at the level of the main portal vein. (F) A widely patent TIPS stent with brisk flow of contrast has been created. As expected, residual flow of contrast into the right portal vein is present, thereby ensuring portal venous supply to the right liver lobe. The ICE catheter tip is again faintly seen within the inferior vena cava, at the level of the main portal vein. For this patient a Viatorr 6+2cm stent (Gore medical, Flagstaff, Arizona, USA) was placed and dilated using an 8mm high-pressure balloon. The portal venous-atrial gradient was decreased from 9 mm Hg to 4mm Hg. ICE, intracardiac echocardiography.",yes
PMC9793602,Figure_8,oa_package/d5/c0/PMC9793602.tar.gz,"[' 8).', 'NLRP3, caspase-1, GSDMD, and IL-1 protein expression in granulosa cells of each group.', '01 compared with the Con groupDiscussionThe findings of the present study indicate that quercetin supplementation protects the ovarian reserve from CTX-induced ovarian damage, resulting in the elevation of serum AMH, E2, and P levels, reduction of FSH and LH expression, and alleviation of ovarian pathology.']","Fig. 8 NLRP3, caspase-1, GSDMD, and IL-1 protein expression in granulosa cells of each group. Original blots/gels are provided separately. protein band, ( ) protein expression of NLRP3, ( ) protein expression of Caspase-1, ( ) protein expression of GSDMD, and ( ) protein expression of IL-1. NLRP3: NOD-like receptor pyrin domain containing 3; GSDMD: gasdermin D. All data are expression as meanstandard deviation mean (xSD) ( =3). <0.05 and <0.01 compared with the CTX group; <0.05 and <0.01 compared with the Con group",yes
PMC6507344,Figure_3,oa_package/0b/05/PMC6507344.tar.gz,"['01; A) and nuclear NF- B was significantly increased by 1.', '001; B) in the A group.', '05, respectively; A), and decreased the expression level of nuclear NF- B compared with the A group (P 0.', '05, respectively; B).', 'Immunofluorescence staining demonstrated that NF- B p65 was primarily expressed in the cytoplasm of the cells, and the NF- B p65 expression in the nucleus was increased in the A group compared with the control group (C).', '.']","Figure 3. Scue inhibits the NF-B signaling pathway. Cytoplasmic expression of (A) NF-B p65 and (B) nuclear NF-B p65. Gray scale analyses were conducted using either (A) -actin or (B) lamin A as an internal reference. Data are presented as the mean standard deviation. n=6. (C) Distribution of NF-B p65 in the cytoplasm and the nucleus were detected by immunofluorescence. NF-B p65 expression appeared green under a fluorescent microscope and the nucleus was stained blue. Scale bar, 20 m. Images are representative of repeated experiments. **P<0.01, ***P<0.001 vs. the control group; P<0.05, P<0.01 vs. the A group. NF-B p65, nuclear factor -light-chain-enhancer of activated B cells p65; Scu, scutellarin; Scue, scutellarein; A, amyloid .",yes
PMC3720563,Figure_2,oa_package/a3/f3/PMC3720563.tar.gz,"['RNO concentration-dependently rescued this reduction by 50% ( panels A and B).', 'This cilia loss was consistent with the results obtained for the gene expression of the ciliagenesis regulator FOXJ1 and the dynein DNAI2 ( panels C and D respectively).', 'g002RSV compromised the number of -tubulin IV labeled ciliated cells and the expression of Foxj1 and Dnai2: Reversal by roflumilast N-oxide.']",10.1371/journal.pone.0069670.g002,yes
PMC9733815,Figure_5,oa_package/4a/4d/PMC9733815.tar.gz,['Malignant peripheral nerve sheath tumor biopsyPane A (left) shows the tumor biopsy slide at 20x magnification using hematoxylin and eosin staining.'],Figure 5 Malignant peripheral nerve sheath tumor biopsy Pane A (left) shows the tumor biopsy slide at 20x magnification using hematoxylin and eosin staining.The black asterisks (*) indicate areas of disorganized cell growth and palisading necrosis. Pane B (right) shows the tumor biopsy slide at 40x magnification using hematoxylin and eosin staining.The black arrows indicate cells in multiple stages of mitosis.,yes
PMC5009183,Figure_2,oa_package/75/83/PMC5009183.tar.gz,"['For the experiments shown in , the outline is depicted in ', 'A schematic representation of seven alternative protocols is shown in A.', 'We collected the cells at day 12 and analyzed the expression of early (Myogenin) and late (MYH1) muscle markers by real-time quantitative PCR (B).', 'We confirmed this hypothesis by analyzing MYH1 protein levels by immunostaining (C and D).', 'We further characterized cells differentiated with protocol 5 for the expression of other myogenic markers, such as DYSTROPHIN (DMD), MEF2C and endogenous MYOD detected with human-specific primers (E).', 'As shown in F, a peak of the expression of the mesoderm marker Brachyury (T) at day 5 is followed by a maximum of endogenous MYOD expression at day 8.', 'Human myoblasts displayed higher levels of MYOD and MYH1, while Myogenin and DMD were more abundant in iPSC-derived muscle cells (G).', 'Finally, as differentiating cells expressed endogenous MYOD (E G), and endogenous MYOD is expected to decrease at the end of differentiation, we removed doxycycline in the last 3 days to allow a more physiological maturation of muscle cells.', '', '(A) Left panel: dose-response relation of ACh-evoked currents in skeletal muscle cells differentiated from WT I-MyoD iPSCs with the protocol 5 described in (*, p 0.', '(B) Average fluorescence response to ACh (300 M), in Fura-2-loaded skeletal muscle cell differentiated from WT iPSC with the protocol 5 described in (n = 22).']","Fig. 2 Optimization of the differentiation protocol. ( ) Diagrams of the variants of the differentiation protocol of WT I-MyoD cells. HUESM: iPSC differentiation medium; GM: myoblast growth medium; DM: myoblast differentiation medium. Red line: time in doxycycline (Dox). Time points of medium change are indicated above. See text for details. (B) Real-time qRT-PCR analysis of Myogenin (white bars) or Myosin Heavy Chain (MYH1; black bars) in cells differentiated as depicted in panel (A). Relative levels of mRNA were calculated with the delta delta Ct method and condition A is used as the calibrator sample. Error bars: standard error from a triplicate. (CD) Immunostaining for the muscle marker MYH1 in WT I-MyoD cells differentiated with protocol A (panel C) or 5 (panel D). Nuclei are counterstained with DAPI. Scale bar for both panels: 50m. (E) RT-PCR analysis of muscle markers in WT I-MyoD cells differentiated with protocol 5 in absence (Dox) or presence (+Dox) of doxycycline. GAPDH is used as a housekeeping control. (F-G) Real-time qRT-PCR analysis of the indicated markers in a time-course experiment using protocol 5 (panel F) and in differentiated iPSCs (protocol 5, day 11) compared with differentiated human myoblasts (panel G). Relative levels of mRNA were calculated with the delta delta Ct method. In panel F, for each marker the condition with higher expression has been used as the calibrator sample. In panel G, differentiated myoblasts represent the calibrator sample. Error bars: standard error from a triplicate.",yes
PMC3823389,Figure_6,oa_package/ea/00/PMC3823389.tar.gz,"['This number was first been proposed by Healey and Schroy[28] [], then by Couinaud,[29] Healey,[30] and Bismuth.', ""\nHealey and Schroy's concept:[28] 2 hemilivers, 4 sectors, 8 segments, but exclusive of the caudate lobe.""]","Figure 6 Healey and Schroy's concept:[ ] 2 hemilivers, 4 sectors, 8 segments, but exclusive of the caudate lobe. a) Original illustration showing 4 sectors (a posterior, anterior, medial, and lateral one) in an anterior view, with a superior and inferior area for each, b) reconstruction on our corrosion cast.",yes
PMC2923787,Figure_1,oa_package/fd/2c/PMC2923787.tar.gz,"['Chest CT scan disclosed a slight enlargement of the mediastinal lymph nodes, centrilobular nodules, thin-walled cysts, the thickening of the bronchovascular bundles, and ground-grass opacities, all of these findings were compatible with those of lymphocytic interstitial pneumonia (LIP; A) (5, 9).', 'The enlargement of mediastinal lymph nodes and abnormal shadows were also partially alleviated (B, C).', 'Effects of cycloheximide and cyclosporine in normal and lipopolysaccharide-treated miceJ Immunol1993150342352841946716PhamLVTamayoATYoshimuraLCLin-LeeYCFordRJConstitutive NF-kappaB and NFAT activation in aggressive B-cell lymphomas synergistically activates the CD154 gene and maintains lymphoma cell survivalBlood2005106394039471609987317MiltenyiZTothJGondaATarIRemenyikEIllesASuccessful immunomodulatory therapy in castleman disease with paraneoplastic pemphigus vulgarisPathol Oncol Res2008153753811906724118TsujimuraSSaitoKNakayamadaSNakanoKTanakaYClinical relevance of the expression of P-glycoprotein on peripheral blood lymphocytes to steroid resistance in patients with systemic lupus erythematosusArthritis Rheum200552167616831593407719TanakaYTsujimuraSMulti-drug resistance in the treatments of autoimmune diseasesJpn J Clin Immunol200629319324Chest CT findings (A) just before the second regimen, of tocilizumab in combination with corticosteroid and tacrolimus: centrilobular nodules, thin walled cysts, the thickening of the bronchovascular bundles and ground-grass opacities were noted; (B) Thirteen months after the continuation of the second regimen; (C) after twenty three months: most of the lesions had alleviated.']","Fig. 1 Chest CT findings ( ) just before the second regimen, of tocilizumab in combination with corticosteroid and tacrolimus: centrilobular nodules, thin walled cysts, the thickening of the bronchovascular bundles and ground-grass opacities were noted; ( ) Thirteen months after the continuation of the second regimen; ( ) after twenty three months: most of the lesions had alleviated.",yes
PMC9072154,Figure_9,oa_package/8c/f9/PMC9072154.tar.gz,"[' 9).', 'Hemolytic uremic syndrome in a 3-year-old boy presenting with bloody diarrhea, thrombocytopenia and renal failure after eating an undercooked hamburger.', 'Note the bladder (B), which is collapsed in the setting of oliguriaOther immune-mediated conditionsA variety of immune-mediated conditions can affect the bowel throughout childhood and they range in presentation from malabsorption to acute systemic inflammation.']","Fig. 9 Hemolytic uremic syndrome in a 3-year-old boy presenting with bloody diarrhea, thrombocytopenia and renal failure after eating an undercooked hamburger. Transverse color Doppler US image of the rectosigmoid colon demonstrates marked circumferential mural thickening and partial loss of stratification without associated hypervascularity ( ) during the initial avascular phase of the disease. Note the bladder ( ), which is collapsed in the setting of oliguria",yes
PMC8304165,Figure_6,oa_package/54/b8/PMC8304165.tar.gz,"['The hSMt sections (A C) show how human skeletal muscle appears in phase contrast microscopy, with the longitudinal view of the skeletal muscle fibers.', 'The results of immunofluorescence analysis revealed no staining of CaSR in hSMt sections (D,F).', 'In comparison to that, positive detection of CaSR expression in human parathyroid tissue sections validated our method of analysis (G,I).', 'The results demonstrate the absence of CaSR protein expression in hSMts (E,H).', 'Human skeletal muscle tissue (hSMt) sections; observations in phase contrast microscopy, original.']","Figure 6 Human skeletal muscle tissue (hSMt) sections; observations in phase contrast microscopy, original. Magnification: 10, scale bar 200 m ( ). Immunostaining of CaSR protein. Analysis of CaSR protein in human skeletal muscle tissue sections: ( ) negative control only with secondary antibody, ( ) control with anti-CaSR antibody absorbed with blocking peptide, and ( ) with primary anti-CaSR antibody. Analysis of CaSR protein in human parathyroid tissue sections used as positive control: ( ) negative control with only secondary antibody, ( ) control with anti-CaSR antibody absorbed with blocking peptide, and ( ) with primary anti-CaSR antibody. Fluorescent microscopy in conventional colors: red for CaSR and blue for nuclei, original magnification: 10, scale bar: 200 m. Experiment was carried out in triplicate (in hSMt sections of three different humans).",yes
PMC6883378,Figure_4,oa_package/5f/9a/PMC6883378.tar.gz,"['In addition, attenuation of the ellipsoid zone was seen ( ()).', 'Horizontal spectral domain optical coherence tomography image through the fovea of the right eye one month after Ranibizumab therapy shows reduction of the central foveal thickness and complete resolution of the cystoid changes.']",Figure 4 Horizontal spectral domain optical coherence tomography image through the fovea of the right eye one month after Ranibizumab therapy shows reduction of the central foveal thickness and complete resolution of the cystoid changes. Few hyperreflective dots corresponding to hard exudates are still seen and central loss of ellipsoid zone is seen centrally (between open arrows).,yes
PMC9510086,Figure_2,oa_package/bd/1a/PMC9510086.tar.gz,"[' 2: A.', 'LSG labial salivary gland, pSS primary Sj gren s syndrome, FS focus score, NSCS non-specific chronic sialadenitisWe investigated the clinical and histopathological features of 217 pSS patients with sufficient LSG tissues for pathological evaluation, especially focusing on patients with NSCS.', ' 2C).']","Fig. 2 Hematoxylin and eosin-stained LSG tissues from pSS patients and a sicca NSCS control. A pSS patient with 0A muciniphila relative abundance correlates with BDNF levels and persistent neuroinflammationA high abundance of A muciniphila has been linked to decreased inflammation in chronic diseases.', '68,69 This activation of the NLRP3 inflammasomes was followed by increased IL-1 and IL-18 levels in the brain (A to D), which might contribute to the persistent neuroinflammation in GWI.', '73 In this study, we report low levels of BDNF in the frontal cortex of mice, which were treated with GW chemicals compared with controls even 5 months post-exposure (F and G).']","Figure 5. GW chemical exposure is associated with chronic neuroinflammation and decreased brain-derived neurotrophic factor (BDNF) levels in frontal cortex. (A, C, E) Immunohistochemistry micrographs of frontal cortex tissues of GWP and CONT treated mice showing immunoreactivity of IL-1, IL-18, and IL-6 (magnification 20 and scale bar 50m). (B, D, F) Morphometric analysis (as % ROI) obtained from 10 to 15 images from different microscopic fields from each mouse sample. (* <.05; n=6). Data are represented as meanSEM. (G) Western blots of BDNF protein levels in the frontal cortex of GWP and CONT treated mice. (H) Morphometry analysis of immunoblots normalized against -actin (n=5) (* <.05; n=5). Data are represented as meanSEM. (I) BDNF immunoreactivity. Representative immunohistochemistry micrographs showing BDNF in frontal cortex tissues of GWP and CONT treated mice (magnification 40 and scale bar 50m). (J) Morphometric analysis (represented as % ROI) obtained from 10 to 15 images from different microscopy fields from each mouse sample (* <.05; n=6). Data are represented as meanSEM. BDNF indicates brain-derived neurotrophic factor; GW, Gulf War; SEM, standard error of the mean; ROI, region of interest.",yes
PMC11378698,Figure_1,oa_package/3a/2f/PMC11378698.tar.gz,['Sagittal scan with iodinated contrast injection.'],"Figure 1 Sagittal scan with iodinated contrast injection. An isthmocorporal uterine parietal breach (star), communicating the uterine cavity with the pelvis.",yes
PMC10438012,Figure_3,oa_package/36/f3/PMC10438012.tar.gz,"['Compared to a normal age-matched control, expression of synaptophysin (SYP), a protein localized to synaptic vesicles of presynaptic compartments of all types of synapses, was greatly depleted in the thalamus, midbrain, pons, and medulla of the AstV-ND-1-NIH patient ().', 'Importantly, synaptophysin expression was lost in the neuropil surrounding AstV-infected neurons (B 3H).', 't001g"" position=""float""/>Biological processEnriched gene ontology termsProtein marker expressionCellular morphology/topology/functionRepresentative images\nDisruption of synapse organization\nsynapse organization (GO:0050808)synapse (GO:0045202)presynapse (GO:0098793)synaptic vesicle (GO:0008021)neurotransmitter secretion (GO:0007269)Neurotransmitter release cycle (REAC:R-HAS-112310)Abnormality of CNS electrophysiology (HP:0030178)Upper motor neuron dysfunction (HP:0002493)SYP (Expressed in synaptic vesicles;pan presynaptic protein marker)- decrease in SYP in the neuropil surrounding AstV-infected neurons;- disruption of structural integrity of presynaptic compartments;- disruption of afferent innervation of infected neurons\nB 3H\n\nDisruption of excitatory synaptic transmission\nchemical synaptic transmission (GO:0007268)glutamatergic synapse (GO:0098978)presynapse (GO:0098793)VGLUT1 (Expressed in presynaptic compartments of glutamatergic synapses)- decrease of VGLUT1 presynaptic puncta in infected brain structures;- disruption of structural integrity of presynaptic compartments of glutamatergic synapses;- disruption of excitatory afferent innervation of infected neurons\nSupplementary .', '.']","Figure 2. Inflammatory mediators of liver cirrhosis (LC) and sarcopenia. (A, B) Enzyme-linked immunosorbent assay was performed to determine the serum levels of lipopolysaccharides (LPS), interleukin-6 (IL-6), and tumor necrosis factor- (TNF-). (A) Serum LPS levels were compared between the control (n=6) and LC (n=4) groups. Serum IL-6 levels were compared between the control (n=4) and LC (n=4) groups. (B) Serum TNF- levels were compared between the control (n=4) and LC (n=4) groups. (C-E) Correlation analyses were performed between serum TNF level and myostatin expression (C, n=8), muscle weight (D, n=8), and myofiber diameter (E, n=8). n.s, not significant. <0.05.",yes
PMC11628433,Figure_1,oa_package/52/48/PMC11628433.tar.gz,"[' 1).', '', ' 1Ultrasound scan of PASHIn one third of cases (30,8%) round or oval, heterogeneous, hypoechoic masses with clusters of microcalcifications were described.']",Fig.1 Ultrasound scan of PASH,yes
PMC6110760,Figure_2,oa_package/1d/43/PMC6110760.tar.gz,"[' 2b).', ' 2a, b).', ' 2b).', 'Detection and quantification of ribitol, ribitol-5P, and CDP-ribitol by LC/MS-MS.', '05To address the question whether the orally administrated ribitol is, in fact, converted to ribitol-5P and CDP-ribitol, we treated differentiated C2C12 myotubes with isotopically labeled 13C5-ribitol (13C-ribitol) in vitro.']","Fig. 2 Detection and quantification of ribitol, ribitol-5P, and CDP-ribitol by LC/MS-MS. LC/MS-MS detection of ribitol, ribitol-5P, and CDP-ribitol from heart and quadriceps of 4-week-old mice treated with drinking water only (untreated) or water supplemented with 5% ribitol for 1 month. Quantification of ribitol, ribitol-5P, and CDP-ribitol levels by LC/MS-MS from heart (H) and quadriceps (Q) of untreated and 5% ribitol-treated mice, and untreated C57 control mice ( =4 for all cohorts). Box represents 25th and 75th percentiles. Line represents median and + represents mean. Whiskers extend from min to max value. Unpaired test, * 0.05",yes
PMC7513510,Figure_1,oa_package/7d/48/PMC7513510.tar.gz,"['1) in six and one patients, respectively.', 'Table 3Pathological features and Immunohistochemical stains (Primary site)CaseFIGO stageLVSI(Ly V)GradeMytosis/10 HPFSarcomatouscomponentSOMyoinvasionSMARCA4BCORERPRCD10P53MIB-1(%)Cyclin D1CytokeratinDesminSMAotherRecurrent cases1IC+ +High5homologous+ 1+ Over express70 CD34( )S100( )2IB High10 15homologous+ 1/2++ +Wild type50+ HHF35( )CD34( )S100( )3IB High10homologous+ 1/2+ +Over express50 ND++HHF35(+)4IC+ High30heterogenous+ 1/2+ND NDOver expressND NDNDNDNon-recurrent cases5IB High5homologous+ 1/2+ +Wild type30+ +ND6IB Low3homologous 1/2+ ++ Wild type5 ND ND7IC Low2homologous 1/2+ND++NDNullND+NDNDNDLVSI lymphovascular invasion, ND not done, SO salcomaous overgrowthTable 4Pathological features and Immunohistochemical stains (Recurrent site)CaseERPRCD10P53MIB-1(%)SMAother1 Over express80 2 +Over express50 Desmin( ) HHF35( )Cytokeratin( )S100( ) CD34( )3 +Over express50NDDesmin(+)Case 4.', 'H E: hematoxylin and eosinThe patient of case 6 showed typical microscopic findings of low-grade adenosarcoma (']",Fig. 1 Case 4. H&E staining (original magnification 400). Rhabdoid tumor cells with eosinophilic cytoplasm. H&E: hematoxylin and eosin,yes
PMC5048175,Figure_3,oa_package/f6/fa/PMC5048175.tar.gz,"['This experiment (A) showed a 115 fold increase in PAF synthesis in response to calcium ionophore A23187, 22.', 'In a parallel experiment, purified lipid extracts derived from BLP and LPS treated cells exhibited increased intracellular Ca2+ concentrations in FURA-2 AM labeled PMNs while, equivalent lipid extract from control cells failed to show an increase in intracellular Ca2+ (B).', '2), and stimulation of the synthesis of inflammatory mediator PAF (A).', 'org/1999/xlink"" xlink:href=""srep34666-f2""/>(A) PMNs synthesize PAF in response to distinct endotoxins.']","Figure 3 ( ) PMNs synthesize PAF in response to distinct endotoxins. Isolated PMNs (1010 cells/ml) were treated with control, calcium ionophore A23187 (1M), LPS (5g/ml)+0.5% serum or BLP (5g/ml) in cell culture plates previously coated with 0.2% gelatin and incubated at 37C for 60minutes. A constant amount of [ H]PAF was introduced as internal standard and the lipids were extracted from the PMNs followed by aminopropyl column and HPLC purification as described under Methods. The fractions with PAF-like bioactivity were tested for the intracellular calcium accumulation in FURA-2AM loaded PMNs (See below). The fractions with PAF-like activity were quantified by mass spectrometry as described in Methods. The results are expressed in terms of picograms of PAF synthesized per 10 cells and represents the summation of 1-O-hexadecyl (C ), 1-O-octadecyl (C ), and 1-O-octadecenyl (C ) species. The result is a representative of two independent experiments. ( ) Lipid extracts from PMNs stimulated by either LPS or BLP induced transient increases in intracellular calcium in naive PMNs. Isolated human PMNs were loaded with FURA-2AM and washed twice with HBSS/A to remove unincorporated FURA-2 AM. 2.510 /ml cells were placed in a quartz cuvette and treated with (i) purified lipid fraction from control PMNs, (ii) Purified lipid fractions isolated from LPS treated PMNs (iii) Purified lipid fractions isolated from BLP treated PMNs. The tracings were recorded at dual excitation wavelengths of 340 and 380nm and emission at 510nm as a function of time. The increase in 340/380 ratio is associated with increased intracellular calcium. The result is a representative of two independent experiments.",yes
PMC4571323,Figure_7,oa_package/70/52/PMC4571323.tar.gz,"['Interactions between DHT and both forms of the AR occur at almost identical points of contact, except at the mutated residue (A).', 'Matias et al provided a structural basis for the glucocorticoid responsiveness of the AR LBD double-mutant L701H/T877A (B)168.', 'In three-dimensional space, V730 is located near the coactivator binding site84 and, along with M734 and I737 in the hydrophobic groove, allows the formation of a smoother and flatter surface that permits greater complementarity to FxxLF compared with the LxxLL motif (C).', 'In the structure, bicalutamide adopts a bent conformation within the ligand-binding pocket (D).', 'Unlike in the structures of R1881- and DHT-bound AR, T877 is not involved in hydrogen bonding (D), which may explain the lower binding affinity of bicalutamide compared with R1881 or DHT.', 'org/1999/xlink"" xlink:href=""aps201418f6""/>Structural understanding of disease/drug resistance-related androgen receptor mutations.']","Figure 7 Structural understanding of disease/drug resistance-related androgen receptor mutations. (A) Structural comparison of wild-type (PDB: 1I37) and mutant T877A (PDB: 1I38) AR LBDs in complex with dihydrotestosterone. Key residues involved in hydrogen bonding have been highlighted, with hydrogen bonds indicated by black dotted lines. (B) Structure of the AR LBD double mutant L701H/T877A complexed with 9-fluorocortisol (PDB: 1GS4). (C) Structural overlay of androgen receptor complexed with FxxLF (PDB: 1XOW) and LxxLL (PDB: 1T7F) motif-containing peptides. Residues V730, M734, and I737 are involved in forming hydrophobic contacts with coactivator peptides. Residue V730 was mutated to M730 to demonstrate an enhanced binding of LxxLL peptides. (D) Structure of AR LBD W741L complexed with bicalutamide (PDB: 1Z95). Residues L704, N705, Q711, and R752 form hydrogen bonds with bicalutamide (indicated by dotted lines). Also shown is the wild-type W741 residue (white) to illustrate a possible steric clash between tryptophan and the B-ring of bicalutamide.",yes
PMC4200883,Figure_1,oa_package/20/17/PMC4200883.tar.gz,"['The mucosa was thickened and pale, with short and swollen villi ().', 'Granulocytic sarcoma of the ileum observed by double balloon endoscopy before treatmentGastrointest Endosc79201416616724119505Small-bowel capsule endoscopy frame showing a luminal stricture with thickened mucosa and short, swollen villi.']","Fig. 1 Small-bowel capsule endoscopy frame showing a luminal stricture with thickened mucosa and short, swollen villi.",yes
PMC4282693,Figure_6,oa_package/f3/49/PMC4282693.tar.gz,"[' 6a, b).', '', ' Scale bar equals 10 m\nDiscussionThe genetic basis for DMD has been determined [37 39], but the mechanisms responsible for the decrease in muscle specific force and increased susceptibility to injury are still being clarified.']","Fig.6 Microtubule structure at the NMJ. To study microtubule architecture underlying the NMJ, intact myofibers from the flexor digitorum brevis muscle (FDB) of wild type (WT) and dystrophic ( ) mice were isolated and plated. The NMJ was identified with -Bungarotoxin (BTX, red inset) and the associated microtubule structures (green) were examined by labeling of -tubulin. Labeling shows disorganization of the normal microtubular latticed structure in the myofiber, as described by others. Interestingly, the microtubule network density was also significantly decreased at the NMJ in muscle. White inset panel shows binarization at the region of interest ( ). Quantification was performed on binary images of -tubulin immunohistochemistry ( =2 animals, 5 fibers per genotype; * <0.05). Scale bar equals 10m",yes
PMC7977697,Figure_40,oa_package/de/90/PMC7977697.tar.gz,[],Figure 16: Pleuroparenchymal fibroelastosis in a 48-year-old woman. Coronal CT image shows dense subpleural consolidative abnormality with traction bronchiectasis and marked upper lobe volume loss.,yes
PMC8591944,Figure_2,oa_package/33/ee/PMC8591944.tar.gz,"[' 2a).', 'a Port placement and operative incision of three-port LRC with an ileal conduit; b Port placement and operative incision of five-port LRC with an ileal conduitConventional five-port LRC with an ileal conduit was performed in accordance with a previously described methom [9], including PLND, RC, and the use of a conventional ileal conduit.', ' 2b).']",Fig. 2 Position of the trocars and incisions for three-port LRC and five-port LRC. Port placement and operative incision of three-port LRC with an ileal conduit; Port placement and operative incision of five-port LRC with an ileal conduit,yes
PMC9364449,Figure_2,oa_package/0c/36/PMC9364449.tar.gz,[],Figure 2 Screen shot of the mirrored image on the PC,yes
PMC4938449,Figure_13,oa_package/f9/e5/PMC4938449.tar.gz,[],"Figure 13 Anteroposterior and lateral radiography of the right kneeof a female patient with a primary breast tumor. Metaphyseal lyticlesion on the tibia, with an imprecise zone of transition andill-defined borders (arrows). Another case of a femalepatient with a primary breast tumor, who presented with knee pain. Theimage shows radiological features of aggressiveness, characterized bylytic lesion on the distal left femur, which breaks through the corticalbone (arrows), invades the adjacent soft parts, without a sclerotic rimand with an imprecise zone of transition. There are also lesions in themedial condyle of the fmur and proximal third of the tibia.",yes
PMC2908353,Figure_4,oa_package/e5/3d/PMC2908353.tar.gz,"['During injection, a series of scans were obtained [] to make sure the cement was heading in the right direction.']",Figure 4 a: xial view after cement injection b: Coronal view after cement injection,yes
PMC9566666,Figure_2,oa_package/50/36/PMC9566666.tar.gz,[],Figure 2 CT transverse view demonstrating obstructive ureteral calculi CT:computed tomography,yes
PMC8619211,Figure_3,oa_package/11/ac/PMC8619211.tar.gz,"['A comparison of growth rates for WT, vps13 , arf1-3 arf2 , and arf1-3 arf2 vps13 strains on L-canavanine (CAN)-containing plates showed that arf1-3 arf2 is even more sensitive to this compound than the vps13 strain and that this sensitivity is increased further in the arf1-3 arf2 vps13 strain (a).', 'However, the amount of CPY secreted by the arf1-3 arf2 vps13 strain was not higher than the amount observed for the vps13 strain (b).', 'Vps13 and Arf1 proteins do not cooperate in endocytosis, but do cooperate in sorting proteins at the Golgi.']","Figure 3 Vps13 and Arf1 proteins do not cooperate in endocytosis, but do cooperate in sorting proteins at the Golgi. ( ) Sensitivity to L-canavanine (CAN) of WT and , , and strains. Serial (4) dilutions of yeast cultures were spotted onto SC or SC-arg supplemented with CAN and incubated at 33 C for 5 days. ( ) Vps13 cooperates with Arf1 in CPY sorting at the Golgi. Overnight yeast cultures were diluted to OD 0.5, cultured in YPD medium at 35 C for 1.5 h, and then harvested by centrifugation. The supernatants, containing secreted CPY, were concentrated on filters. Protein extracts from the cell pellets were obtained to determine the Pgk1 level (used as a control for the number of cells). Both fractions were analyzed by Western blot using -CPY and -Pgk1 antibodies. Experiments were performed in triplicate. Densitometry analysis of the blots was performed and presented in graphical form; (AU)arbitrary units. Test- : nsnon-significant; * < 0.05; ** < 0.01.",yes
PMC4248060,Figure_4,oa_package/43/99/PMC4248060.tar.gz,"['As shown in , a lesion of lung cancer had different features on the CT image and the 18F-FDG-PET image.', 'A lesion of lung cancer shown on CT image (on the left) and on 18FDG-PET-CT image (on the right)Biological target volumes are tumour targets with different radio-sensitivity in the region determined by a series of biological factors.']",Fig. 4 A lesion of lung cancer shown on CT image (on the left) and on 18FDG-PET-CT image (on the right),yes
PMC10705034,Figure_3,oa_package/1b/ee/PMC10705034.tar.gz,"['Intra-Operational Arthroscopy Image Showing Foreign Bodies in Medial Gutter Intra-Operational Arthroscopy Image Showing Foreign Bodies in Intra-Articular ViewIntra-Operational Arthroscopy Image Showing Intact MenisciPost-operatively, weight-bearing was advised as tolerated along with baby aspirin for two weeks.']",Figure 3 Intra-Operational Arthroscopy Image Showing Foreign Bodies in Medial Gutter,yes
PMC7559652,Figure_1,oa_package/85/47/PMC7559652.tar.gz,['Right thyroid sonogram.'],"Figure 1 Right thyroid sonogram. A: Left thyroid sonogram; B: Both A and B show multifocal, hypoechoic, irregular, and calcified nodules in the thyroid gland (orange arrows).",yes
PMC3377938,Figure_8,oa_package/4a/54/PMC3377938.tar.gz,[],"Figure7 MHV ns2 Facilitates the Induction of Viral Hepatitis in (A) Livers sections from mock or virus-infected mice sacrificed at 5 and 7 d.p.i. were stained with hematoxylin and eosin (H&E). Arrows show areas of necrosis. The scale bar represents 500m. Sections are representative of two sections from three animals from each group. (B) Liver sections from mice sacrificed at 5 d.p.i. were stained with H&E for detection of inflammation or with anti-caspase-3 antibody for detection of apoptosis. The scale bars represent 100m. Sections are representative of two sections from three animals from each group. (C) Livers were harvested from mock- or virus-infected mice (n= 5) at 3, 5, and 7 d.p.i., and RNA was isolated. TNF- and IFN- mRNAs were quantified by qRT-PCR, normalized to -actin mRNA level, and expressed as the fold change relative to mock infected with the formula 2 .The error bars represents the SEM. Asterisks indicate that differences are statistically significant ( p< 0.05). See also .",yes
PMC9433881,Figure_2,oa_package/a4/ae/PMC9433881.tar.gz,"['The mean FC matrices show the correlation of neuronal calcium signals between brain regions (s 2A and 2B).', '01) ( 2C).', '0015) (s 2D and 2E).', ' 2Modulation of astrocyte calcium activity alters network FC in healthy C57BL/6 mice(A E) The effect of astrocyte modulation (mCherry, DREADDs, or P130PH under the GFAP promotor) on widefield calcium imaging of neurons, which were transfected with GCaMP6f after intravenous (i.', 'Similarly, DREADDs and P130PH increased or decreased BOLD FC, measured with rsfMRI, between the anterior and posterior cingulate cortex, respectively, as shown on the mean FC matrices and FC map of the anterior cingulate cortex (s 2F 2H).', '0001) ( 2I).', '0002, N = 7/group) after AAV expression ( 2J).', '002) ( 2K).', '5"">Recovery of astrocyte dysfunction mitigates clinically relevant phenotypesOur rsfMRI data confirm earlier results ( 2) showing that the activation of astrocytes in AppNL control mice increased BOLD FC (Z scores for AppNL controls mCherry 0.']","Figure1 Increased FC of the anterior cingulate cortex in the human brain and mice (A) Amyloid load (CL values SEMs) for controls (N= 24) and amyloid accumulators (N= 10). Threshold at CL= 23.5 is indicated by the dotted line. p<0.001, 2-way repeated ANOVA with Sidak correction. (B) Mean FC matrices of controls (N= 24) and amyloid accumulators (N= 10). p<0.001, 2-sample t test, FDR corrected. Color scale shows scores. (C) Mean BOLD FC map of the anterior cingulate cortex of controls and amyloid accumulators (FDR corrected, p<0.05). Color scale shows t values (i.e., FC between the anterior cingulate cortex and all other voxels in the brain). Statistical map shows anterior cingulate connections that are increased in amyloid accumulators versus controls, 2-sample t test, uncorrected, p<0.001. (D) BOLD FC ( scores SEMs) between the anterior and posterior cingulate cortex. p<0.001, 2-sample t test. (E) Correlation between change in amyloid load (CL between baseline and time point 1) and BOLD FC (between the anterior and posterior cingulate cortex) at baseline for controls (r= 0.14, p= 0.52) and amyloid accumulators (r= 0.86, p= 0.0016). (F) Mean FC matrices of 3-month-old control (N= 17) and mice (N= 14). p<0.05, p<0.01, p<0.001, 2-sample t test, FDR corrected. Color scale shows scores. (G) Mean BOLD FC map of the anterior cingulate cortex of control and mice (FDR corrected, p<0.05). Color scale shows t values. Statistical map shows anterior cingulate connections that are increased in mice versus controls, 2-sample t test, FDR corrected, p<0.05. (H) BOLD FC ( scores SEMs) between the anterior and posterior cingulate cortex. p<0.001, 2-sample t test.",yes
PMC11488446,Figure_2,oa_package/c9/f7/PMC11488446.tar.gz,"['Detailed illustrations are shown in .', 'Alterations in the WNT/PCP Signalling Pathway During the Progression of OA.', ')11 This process allows more tagged molecules per target and was used with the initial studies on MyoD IHC (); however, frozen sections were required.', '1-2015-001"" position=""float"">Indirect immunofluorescent stain of MyoD in alveolar soft part sarcoma.']","Figure 3 Indirect immunofluorescent stain of MyoD in alveolar soft part sarcoma. With this technique, the fluorescein dye molecule is attached to a secondary antibody directed against the primary one, ie, goat anti-rabbit immunoglobulin, which in turn binds to the primary rabbit anti-MyoD polyclonal antiserum. This globoid pattern of positivity highlights a major problem with immunofluorescence as compared with PAP stains: the lack of cellular definition. Because of the configuration, this staining pattern was originally interpreted as nuclear, but in fact, it resulted from MyoD-positive cytoplasmic globules.",yes
PMC6219843,Figure_13,oa_package/7d/f9/PMC6219843.tar.gz,[],10.7554/eLife.40202.015,yes
PMC3391882,Figure_9,oa_package/e5/b0/PMC3391882.tar.gz,[],Fig. 9 Local aponeurotic repair,yes
PMC7683281,Figure_2,oa_package/73/ce/PMC7683281.tar.gz,"['A.', 'Ileocecetomy was perfomed with intracorporeal ileo-cecal side-to-side anastomosis by Endo GIA 60 mm blue (']","Fig. 2 A. ileo-cecal intussusception seen during laparoscopic exploration (appendex: red arrow, terminal ileum: blue arrow, colon: black arrow). B: image showing edematous cecal wall and mesentery during laparoscopic reduction of the fore mentioned intussusception. (Cecum: black arrow, ileum: blue arrow). C: Colo-Colic Intussusception seen on laparoscopy (black arrow: ascending colon, Blue arrow: cecum). D: Laparoscopic view of the right colon post-reduction of ileo-ceco-colic intussusception. E: Mechanical ileo colic intracorporeal side-side anastomosis after laparoscopic ileo-cecectomy using endoGIA blue 60 mm.",yes
PMC9907162,Figure_1,oa_package/23/d9/PMC9907162.tar.gz,"['The ED may be used to compare doses from different imaging modalities ().', 'It is expressed by the following formula [20]:Scheme of determining the effective dose\nE = WTHT= WT WRDT,R\nwhere wT is the tissue weighting factor for tissue or organ T , HT is the equivalent doses to the tissues or organs, wR is the radiation weighting factor, and DT,R is the mean absorbed dose from radiation R in a tissue or organ T.', ' shows how to calculate the ED.']",Figure 1 Scheme of determining the effective dose,yes
PMC10506824,Figure_6,oa_package/6d/01/PMC10506824.tar.gz,"['The dermoscopic evaluation during treatment process helps to identify decreased vascularity and stability in disease progress, and choose an appropriate time to initiate QSNY lasers, and during and after laser sessions, it helps to assess satisfactory response to therapy [].', '(a) Clinical image of lichen planus pigmentosus (LPP) before treatment.']","Figure 6 (a) Clinical image of lichen planus pigmentosus (LPP) before treatment. (b) Clinical image of LPP after treatment with QS: Nd YAG laser. (c) Dermoscopic image of LPP right cheek stable disease shows perifollicular and perieccrine bluish pigment globules (orange arrow) with follicular sparing. (d) Dermoscopic clearance after single laser session of 1064 QS laser spot size 6 mm, 2.4 mj, evaluated at 1 year after one session, shows marked decrease in pigment globules (blue arrow). (e) Dermoscopic image of left cheek LPP shows perifollicular and perieccrine bluish pigment globules (orange arrow) with follicular sparing. (f) Dermoscopic image of improvement left cheek after single session of 1064 QS laser spot size 6 mm, 2.4 mj, evaluated at 1 year after one session. Dermoscopy shows marked decrease in pigment globules (blue arrow). (Fotofinder handyscope, non-polarised,10x)",yes
PMC9887544,Figure_3,oa_package/f2/b6/PMC9887544.tar.gz,[' Magnetic resonance imaging (MRI) brain diffusion-weighted imaging (DWI) sequence showing gyriform foci of restricted diffusion in keeping with acute watershed infarcts as a result of earlier cerebral air emboli.'],Figure 3 Magnetic resonance imaging (MRI) brain diffusion-weighted imaging (DWI) sequence showing gyriform foci of restricted diffusion in keeping with acute watershed infarcts as a result of earlier cerebral air emboli.,yes
PMC10644142,Figure_6,oa_package/c9/5c/PMC10644142.tar.gz,"['US shows fluid deep to the myotendinous junction of the medial gastrocnemius and superficial to the soleus muscle with disruption in contour and echogenicity of muscle fibers of the distal medial gastrocnemius () (27).', 'Tennis leg of the right calf in a 61-year-old tennis player.']","Figure 6 Tennis leg of the right calf in a 61-year-old tennis player. (A) Longitudinal US and (B) panoramic view shows an anechoic fluid collection deep to the medial gastrocnemius and superficial to the soleus muscle. There is disruption of the muscle fibers of the distal medial gastrocnemius. US, ultrasound.",yes
PMC4052880,Figure_1,oa_package/bc/3a/PMC4052880.tar.gz,['Freehand SPECT method.'],"Figure 1 Data acquisition using the freehand SPECT device; radioactivity is measured with the probe from multiple directions. is the probe, and in yellow, the detection beam of the probe. The orange cloud is an accumulation of the area where it is measured. The localisation of the I seed after reconstruction. The I seed reconstruction is projected over the optical image in purple. is the patient tracker. 3D visualisation of the distance and direction of the probe tip to the I seed. Injection of Tc-nanocolloid guided by freehand SPECT. is the tracer injection localisation.",yes
PMC4947121,Figure_2,oa_package/60/48/PMC4947121.tar.gz,"[' 2a, b).', ' 2a, arrows), whose levels were very low in non-treated cells, showing its particularly high sensitivity to lysosomal degradation.', ' 2c) and similarly to lamp1, they localized at their membranes (', ' 2c, arrow).', ' 2c).', ' 2d) and in cells stably expressing this enzyme (', ' 2e, f), as compared to mock-transfected cells.', ' 2g).', ' 2h, i).', '', '05 indicate significant differences relative to control cells n = 8, from three independent experiments\nIn vivo treatment of young 3xTgAD mice with the -secretase inhibitor ELND006 triggers massive increases in EAL-associated APP-CTFs and leads to autophagic pathologyWe then questioned whether C99 per se could contribute to lysosomal-autophagic dysfunction.', ' 2a) (see also [56]).', ' 2c).']","Fig.2 In vitro analysis shows that APP-CTFs are degraded by cathepsins through autophagy. , SH-APPswe cells were treated with NH Cl, leupeptin (Leu), pepstatin A (PepA) or PADK and analyzed by western blot using -APPct. correspond to a non-identified 25kDa APP-CTF fragment. in correspond to quantification of C99 and C83 immunoreactivities obtained in and expressed relative to expressions measured in DMSO-treated cells normalized to actin. Data are represented as meanSEM, as determined by ANOVA one-way Dunnett post hoc test, ** <0.01 and * <0.05 indicate significant differences relative to control cells ( =1016, from four independent experiments). Immunocytochemical analysis using -APPct and -lamp1 on H O or NH Cl treated SH-APPswe cells. Note the labeling of -APPct in enlarged -lamp1 vesicles in merged images ( ). SH-APPswe cells were infected with lenti-cathepsin B (CatB) or empty vector (mock) virus at different concentrations (1, 5 or 10l of a stock corresponding to 3.7510 TU/ml) and CatB and APP-CTF levels were analyzed after 48h by western blot. -cathepsin B revealed immature (proCatB) and mature (mCatB) cathepsin B. , CatB and APP-CTF levels were analyzed by western blot in CatB stable cell lines after six passages. in represent the quantification of mCatB and C83 and C99 immunoreactivities obtained in , each expressed as respective expressions measured in mock cells. Data are represented as meanSEM, MannWhitney, *** <0.001 and ** <0.01 ( =7 from three independent experiments). Recombinant C100-flag was incubated in the absence or presence of increasing concentrations of purified CatB at 37C during 0 (To, 50ng) or 60min (5,10 or 50ng) and C100 levels were detected by western blot using -Flag antibody. , SH-APPswe cells were treated with smer-28 (smer), PADK or bafilomycin A1 (BafA1), and APP-CTF levels were analyzed by western blot using -APPct. corresponds to a non-identified 25kDa APP-CTF fragment. in correspond to quantification of C83 and C99 immunoreactivities obtained in and expressed as the percentage of the expressions in DMSO-treated cells normalized to actin. Data are represented as meanSEM, as determined by ANOVA one-way Dunnett post hoc test, ** <0.01 and * <0.05 indicate significant differences relative to control cells =8, from three independent experiments",yes
PMC7754595,Figure_4,oa_package/8d/b9/PMC7754595.tar.gz,"[' 4 shows the distribution of mean-intersection-over-union (mIoU) for the validation set, and various examples of biopsy sites predicted by CAIADS.', 'The accuracy of the CAIADS in predicting biopsy sites.', 'Abbreviations: CAIADS, Colposcopic Artificial Intelligence Auxiliary Diagnostic System; IQR, interquartile rangeDiscussionThe performance of colposcopy and directed biopsies is the major challenge in the cervical cancer screening process.']","Fig. 4 The accuracy of the CAIADS in predicting biopsy sites. Note: We tuned off the displays of internal structure effects to allow more straightforward comparison between CAIADS and ground truth biopsy sites. Left, the distribution of mean-intersection-over-union (mIoU) for the validation set. Right, various examples of CAIADS for predicting biopsy sites. The white and blue circle-shaped sites represented the predicted and ground truth biopsy sites, respectively. Abbreviations: CAIADS, Colposcopic Artificial Intelligence Auxiliary Diagnostic System; IQR, interquartile range",yes
PMC5129911,Figure_6,oa_package/37/c6/PMC5129911.tar.gz,['HAPLN1 silencing leads to mesenchymal and stem cell marker lossReal-time-PCR-assessed mRNA expression of the indicated genes in HepaRG progenitor cells transfected with the indicated siRNAs.'],Figure 6 HAPLN1 silencing leads to mesenchymal and stem cell marker loss Real-time-PCR-assessed mRNA expression of the indicated genes in HepaRG progenitor cells transfected with the indicated siRNAs. HAPLN1 silencing downregulates SNAI1; ACTA2; COL4A1 and LGR5 expression.,yes
PMC2892382,Figure_5,oa_package/f2/5d/PMC2892382.tar.gz,"['Consistent with these results, the integrase inhibitor raltegravir blocked\ninitial infection but not PMA induced gene expression (Supplementary \n5).', '6\nyields some cells expressing Gag and others expressing only GFP (a).', 'To confirm that\nGFP+Gag cells were latently infected, we\ndemonstrated that CD4 downmodulation, which occurs only when HIV Nef, Vpu or\nEnv is expressed, 11 occurred in\nGag+ but not GFP+Gag cells\n(a).', '6\n(b).', 'Moreover, PMA and\nionomycin treatment of Jurkat cells infected with the reporter virus\nincreased Gag+ cell frequency and reduced\nGFP+Gag cell frequency (c).', 'We observed separate populations of Gag+ and\nGFP+ cells in UCB derived CD34+ HPCs\ninfected with the latency reporter virus, indicating that active and latent\ninfection occurred in this cell type (\n5d).', 'In culture, the Gag+ cells were rapidly lost\nwhereas the GFP+Gag cells persisted at least 20\nd (e, Supplementary Fig\n1d).', 'Analysis of these cells revealed that many had a cell surface\nphenotype consistent with primitive HPCs\n(CD34+Lin or\nCD34+CD38 )(f).', 'Active and latent infection in T cells and HPCs.']","Figure 5 Active and latent infection in T cells and HPCs. ( ) Gag, GFPand CD4 expression 7 d after infection in CEMSS cells infected with89.6ESFGFP or89.6EIRESGFP .Histogram shading corresponds to cell gate. ( ) Flow cytometricanalysis of PHAactivated PBMC infected with89.6ESFGFP for 48 h. The histogram is shaded to match the gated cells. In the leftpanel, the isotype control is shown in gray. ( ) Flowcytometric analysis of Jurkat cells infected with89.6ESFGFP for 7 d, then split into PMA and ionomycin or DMSO control for 48 h.( ) Flow cytometric analysis of UCBderivedCD34 HPCs infected with89.6ESFGFP for 3 d. ( ) Time course analysis of Gag andGag GFP UCBderivedCD34 HPCs infected as above and cultured in CC110.( Flow cytometric analysis of UCBderivedCD34 HPCs infected as above and cultured 3 d in CC110medium. Gag cells and Gag GFP cells were gated on the left plot and overlaid (black dots) on plots of CD34vs. Lin (middle panels) or CD38 plots (right panels). The grey backgroundshows the total population.",yes
PMC5544476,Figure_4,oa_package/a1/fc/PMC5544476.tar.gz,"['At six weeks postoperatively, the Kirschner wires were removed, under general anaesthesia, and the patient was encouraged to return to normal footwear ().', '(A) Radiographs following removal of kirschner wires and (B) 12 weeks postoperatively.', 'He had no further issues with his post-operative rehabilitation and had returned to full activity at 3 months and was pain free at 12 months post injury.']",Fig. 4 (A) Radiographs following removal of kirschner wires and (B) 12 weeks postoperatively.,yes
PMC11595750,Figure_2,oa_package/cf/8f/PMC11595750.tar.gz,"['The results of visual examinations of BCT and VNHAP images performed by Operator 1 and Operator 2 were illustrated in and , respectively.', 'Operator 1 s evaluation of standard bone CT images and virtual non-hydroxyapatite maps (VNHAP) compared to the 3-month clinical and imaging follow-up using Fisher s exact test.']",Figure 2 Operator 1s evaluation of standard bone CT images and virtual non-hydroxyapatite maps (VNHAP) compared to the 3-month clinical and imaging follow-up using Fishers exact test. Op. 1 = Operator 1; M+ = positive for fracture; M = negative for fracture; VNHAP = virtual non-hydroxyapatite; bone CT = bone computer tomography.,yes
PMC10791702,Figure_1,oa_package/44/2e/PMC10791702.tar.gz,"['1, Table 1).', '1).', 'Representative images of the assessed histopathologic items.', 'Both ductal proliferation and ductular proliferation (J H E, magnification 100) can be highlighted with IHC for CK7 where ductal proliferations have patent lumen red arrow compared to ductular proliferation forming strings of cuboidal cells in a ductular configuration located outside the limiting plate yellow arrows (K, L IHC, CK7, magnification 400 and 200 respectively)Table 1Demographic, clinical, and laboratory and histopathologic features of 459 infants with neonatal cholestasisFeaturesN (%)FeaturesN (%)Age groupsSex 28 60 Days200 (43.']","Fig. 1 Representative images of the assessed histopathologic items. A 46-day-old girl with a neonatal hepatitis pattern showed mild cytoplasmic cholestasis with multinucleated giant hepatocytes ( H&E, magnification 400). Another liver biopsy from a girl aged 39 days diagnosed with neonatal hepatitis pattern showed evident lobular extramedullary hematopoiesis ( H&E, magnification 200). A 69-day-old boy was diagnosed with galactosemia and his liver biopsy showed moderate steatosis with cholestasis ( H&E, magnification 200). A liver biopsy from a 45-day-old boy showed edematous portal tracts ( H&E, magnification 100), where edema fluid dissects portal collagen around bile duct containing bile plugs ( H&E, magnification 400) and bile ductules encircling a vessel resulting in ductal plate-like malformation ( H&E, magnification 100). Bile ductular proliferation was assessed as mild focal yellow line highlights ductular proliferation in < 50% of portal tract circumference outlined with red ( H&E, magnification 100), mild diffuse in most portal tracts ( IHC, CK7, magnification 40) or moderate to marked ( H&E, magnification 100). Both ductal proliferation and ductular proliferation ( H&E, magnification 100) can be highlighted with IHC for CK7 where ductal proliferations have patent lumen red arrow compared to ductular proliferation forming strings of cuboidal cells in a ductular configuration located outside the limiting plate yellow arrows ( IHC, CK7, magnification 400 and 200 respectively)",yes
PMC6222455,Figure_3,oa_package/0f/65/PMC6222455.tar.gz,"['5 M (A).', 'The IB analysis following IP revealed that increasing concentrations of NiCur reduces the Dox-induced p53K382ac, p53S15p, and p53 levels in a dose-dependent manner (B).', 'Then, to verify whether the diminishing levels of p53K382ac and p53S15p were due to NiCur-mediated decline of p53 protein levels, the immunoblot signal of p53, p53K382ac, and p53S15p for a given condition as mentioned in A were normalized with the respective signal of the control histone H3.', 'Therefore, to examine the effect of NiCur on p21 activation, U2OS cells were treated with Dox alone and together with NiCur, according to the conditions as described in A.', 'IB analysis revealed that increasing concentrations of NiCur inhibits the expression of p53, which negatively affects the induction of p21 protein even after treatment with Dox (C).', 'This enrichment of p53 on p21 promoter was downregulated significantly by NiCur (D).', 'Besides, the levels of H3Kac, which almost doubled after Dox treatment, declined due to the presence of NiCur (D).', 'XS139p) was investigated in U2OS cells after Dox treatment as described in A.', 'XS139p induced by Dox (E).', 'Dose-dependent inhibition of p53 in U2OS cells by NiCur.']",molecules-23-01930-sch002_Scheme 2,yes
PMC2637578,Figure_5,oa_package/8b/ce/PMC2637578.tar.gz,"['After a stable baseline respiration rate was obtained, the NO donor PAPA NONOate (5 M, t1/2=15 min) was added at 160 M O2 ( 80% O2 saturation), which resulted in NO release and a progressive inhibition of respiration (see A).', 'NO traces were corrected for baseline and aligned with the corresponding O2 traces in order to plot O2 consumption rates, calculated as derivatives of the O2 concentration against time traces, and normalized to the maximum respiration rate as a function of NO concentration (see B) [16].', 'Oxygen traces from a representative experiment are shown in (A).', '80% of the initial O2 concentration), the NO donor PAPA NONOate (5 M) was added to the respiration chamber (A).', 'In the absence of the NO donor, a constant rate of O2 consumption was observed until O2 was depleted (A, dashed lines), whereas addition of the NO donor resulted in inhibition of mitochondrial respiration in both control and HFD groups (A, solid lines).', 'Effect of HFD on NO-dependent inhibition of mitochondrial respiration(A) A representative respiration experiment using mitochondria (0.', 'To determine whether there was a difference in the sensitivity of mitochondrial respiration to inhibition by NO between control and HFD groups, respiration rate, calculated as the instantaneous derivative of the O2 concentration versus time traces shown in (A), was plotted as a function of NO concentration, which was recorded following the bolus addition of PAPA NONOate with the NO sensor (not shown) (B).', 'In contrast, at 16 weeks there was a statistically significant increase in the degree of NO-dependent inhibition of respiration in HFD mitochondria compared with controls (B).', '04) (C).', 'Mitochondria isolated from HFD mice required 25% less NO to obtain 50% inhibition of respiration compared with the control group (), which is consistent with our previous studies in the alcohol hepatotoxicity model [16,17].']","Figure 5 Effect of HFD on NO-dependent inhibition of mitochondrial respiration ( ) A representative respiration experiment using mitochondria (0.5mg/ml) from liver of control (black lines) and HFD (grey lines) mice in the presence of succinate/rotenone (15mM/5M) and ADP (0.5mM). Oxygen traces are shown in the presence of PAPA NONOate (5.0M, solid lines), added to the chamber at 80% O (at the arrow) and in its absence (dotted lines). Note that there was no difference in the rate and amount of NO produced between groups (results not shown). ( ) Representative traces of respiration rate, % of maximum, as a function of NO concentration using mitochondria from liver of control (black) and HFD (grey) mice. Both O and NO concentrations are measured simultaneously for the entire duration of each experimental run. ( ) The concentration of NO that causes a 50% decrease in mitochondrial respiration rate (i.e. IC ) for control and HFD groups is shown. Results obtained from traces shown in ( ) (control, black line, IC =solid line and HFD, grey line, IC =dashed line). Values are expressed as the meansS.E.M. for six pairs of mice. * <0.05, compared with control.",yes
PMC6288508,Figure_5,oa_package/e1/64/PMC6288508.tar.gz,['The drainage process during surgery.'],"Figure 5 The drainage process during surgery. A: As soon as the infusion line was opened, a copious, thick flux of blood flowed out of the 20G cannula; B: The 20G cannula was left open throughout the surgery.",yes
PMC4874602,Figure_11,oa_package/80/30/PMC4874602.tar.gz,[],Figure 11 MALDI-MS time course images of BDQ and M2 in C3HeB/FeJ mice acquired8168 h following a single oral 25 mg/kg dose of BDQ. Caseousnecrotic granulomas (type I) are outlined in white. Images depicta single lung lobe obtained from a representative mouse ( = 4 mice per time point). A serial section was stained with hematoxylinand eosin (H&E) for comparison.,yes
PMC10345138,Figure_5,oa_package/a6/9c/PMC10345138.tar.gz,"['5).', '5A, B are representative dark-adapted (DA) and light-adapted (LA) ERG recordings obtained from PN 6-mo mice.', '5C).', '5D) vs.', '5E, F).', '5E).', '5F).', 'Attenuated b-wave amplitudes lead to reduced b/a ratios and slower responses in mutant mice.', 'c-wave and d-wave analysis in WT and K42E retinaPreviously, we observed that targeted ablation of Dhdds in the RPE caused RPE atrophy, retinal degeneration, and reduction of DA and LA ERG [28].', '5E, F), indicating longer bipolar cell recovery time, predicting slower flicker response or lower flicker fusion frequency.']","Fig. 5 Attenuated b-wave amplitudes lead to reduced b/a ratios and slower responses in mutant mice. Representative traces of DA ( ) and LA ( ) ERG responses are shown for WT (black) . K42E mice (gray) at PN 6-mo. The DA b/a ( ) and LA b/a ( ) ERG responses were calculated and plotted over time for PN 1-, 2-, 3-, 6-, 8-, 9-, 12-, and 18-mo. All K42E b/a were significantly different from WT at all time points under both DA and LA conditions. Horizontal dashed line at b/a=1 indicates the negative ERG threshold. Implicit time of a-waves and b-waves were averaged and plotted for DA ( ) and LA ( ) responses. Statistical significance: * 0.05, ** 0.01. K42E PN 1-mo, =9, PN 2-mo, =11, PN 3-mo, =9, PN 6-mo, =15, PN 8-mo, =3, PN 9-mo, =3, PN 12-mo, =7, PN 18-mo, =10; WT PN 1-mo, =10, PN 2-mo, =7, PN 3-mo, =9, PN 6-mo, =14, PN 8-mo, =3, PN 9-mo, =5, PN 12-mo, =6, PN 18-mo, =8.",yes
PMC7448496,Figure_5,oa_package/48/f9/PMC7448496.tar.gz,"[' 5a).', ' 5b).', ' 5c).', ' 5d).', ' 5e).', '\nLBX2-AS1 regulated TRIM28 expression via sponging miR-491-5p.', '001LBX2-AS1 promoted cell proliferation and resistance to cell apoptosis by repression of miR-491-5p.']","Fig. 5 LBX2-AS1 regulated TRIM28 expression via sponging miR-491-5p. The expression of miR-491-5p was detected in U87MG and U251MG cells transfected with miR-NC or miR-491-5p inhibitor. The expression of TRIM28 mRNA was detected in U87MG and U251MG cells transfected with miR-NC or miR-491-5p inhibitor in combination with si-Control or si-LBX2-AS1-1 by RT-PCR. The expression of TRIM28 protein was detected in U87MG cells transfected with miR-NC or miR-491-5p inhibitor in combination with si-Control or si-LBX2-AS1-1 by western blotting. Sequence alignment of LBX2-AS1 TRIM28 3UTR WT, TRIM28 3UTR Mut and miR-491-5p. Luciferase activity was detected in U87MG cells transfected with TRIM28 3UTR WT or TRIM28 3UTR Mut in combination with si-Control or si-LBX2-AS1-1 and miR-NC or miR-491-5p inhibitor. **, p<0.01; ***, p<0.001",yes
PMC5486831,Figure_3,oa_package/ad/f3/PMC5486831.tar.gz,[' 3).'],"Fig.3 Case 6. 39-year-old woman. Symptoms suggestive of red degeneration were not mentioned in the clinical report. Although the time of symptom onset was not estimated precisely, she underwent MRI three times from 2008 through 2011 and typical radiological findings of red degeneration on MRI were observed as early as 2008 ( T1-weighted image, T2-weighted image). Axial T1-weighted image shows an entirely hyperintense lesion compared to myometrium. Axial T2-weighted image shows the lesion, accompanied by a distinct hypointense rim ( ), exhibiting slightly high signal intensity. The lesion is entirely hyperintense compared to myometrium on T1-weighted images, even in 2010 and 2011 ( and , respectively), although the signal intensity in those years is less than that in 2008. A thin, distinct hypointense peripheral rim ( ) is also identified on T2-weighted images in 2010 and 2011 ( and respectively). The signal intensity of the lesion on T2-weighted images in 2011 ( ) is lower than that in 2010 ( ). Gross examination of the lesion resected by laparoscopic myomectomy using a power morcellator shows a homogeneous pink-tan appearance. Photomicrograph of the lesion shows coagulative necrosis similar to that shown in Fig. F",yes
PMC10640493,Figure_1,oa_package/63/e7/PMC10640493.tar.gz,"[' 1a f.', ' 1g).', 'Representative ultrasound images showing four-grade semiquantitative scoring system of salivary gland.', '05 using asymptotic significance 2-sidedData shown in Table 3 illustrates the difference between SSc patients with and without glandular pathology regarding the clinical and laboratory features.']","Fig. 1 Representative ultrasound images showing four-grade semiquantitative scoring system of salivary gland. GSUS of the parotid (grade 2). PDUS of the parotid gland (grade 1). GSUS changes of the submandibular gland (grade 1). PDUS of the submandibular gland (grade 2). GSUS of the submandibular gland (grade 3). PDUS of the submandibular gland (grade 3). Data are expressed as median (interquartile range) for nonparametric variables and frequencies (percentages) for categorical variables. SSc, systemic sclerosis; GS, gray scale; DS, Doppler signal; GSUS, gray scale ultrasound; PDUS, power Doppler ultrasound. A single asterisk (*) denotes significance at <0.0001, compared to the control individuals. The mean difference is significant at <0.05 using asymptotic significance 2-sided",yes
PMC4255369,Figure_6,oa_package/67/0f/PMC4255369.tar.gz,"['The flexibility of the binding site allows DC8E8 to adapt to four homologous, albeit not identical, structural determinants in the tau microtubule-binding repeats.']","Figure 6 Two independently refined X-ray structures of DC8E8 antigen-binding fragment and (stereoview) show the flexibility of the antigen-binding site. The surface of the antibody is shown in grey. The backbones of complementarity determining regions (CDRs) are represented as tubes, with the diameter and colour reflecting their averaged atomic displacement parameters, that is, flexibility. The flexibility is expressed as a colour scale ranging from blue to red, corresponding to B-factors 30 to 150 . The CDRs L1 and H3 exhibit higher B-factors than the remaining parts of the model. The pronounced flexibility of these CDRs is essential to allowing DC8E8 to bind each of four slightly different epitopes within the microtubule-binding repeats (MTBRs). Superposition of both independently refined DC8E8 molecules shows that the core of the binding pockets is invariant (molecule A shown as grey solid, molecule B as blue mesh). The CDR loops (italic letters) create a 7- to 9--deep pocket with surface dimensions 1814 (red axes). The shape of this pocket necessitates that the minimal DC8E8 epitope HXPGGG adopts a fold protruding into this space to bind in the DC8E8 combining site.",yes
PMC9420252,Figure_4,oa_package/8f/22/PMC9420252.tar.gz,"[' 4a), indicating that RIPK3 mediates MLKL activation in diabetic hearts.', ' 4b), attenuated myocardial dysfunction as evidenced by decreased FS% and EF% (', ' 4c, d, Additional file 1: Table S1), reduced myocardial collagen deposition (', ' 4e), and decreased serum biomarkers of myocardial injury (CK-MB and troponin I) in STZ-injected mice (', ' 4f, g).', 'Effects of RIPK3 knockout in streptozocin (STZ)-induced diabetic hearts.', '05 versus STZ + WT (ANOVA)Effects of GSK 872 in streptozocin (STZ)-induced diabetic hearts.']","Fig. 4 Effects of RIPK3 knockout in streptozocin (STZ)-induced diabetic hearts. RIPK3 knockout (RIPK3-KO) and wild-type mice (WT) were rendered diabetic by STZ injection. The levels of phosphorylated MLKL (p-MLKL) were analyzed by western blot in the heart. Upper panel: Upper panel: a representative western blot from 2 out of 6 different hearts in each group for p-MLKL and total MLKL; Bottom panel: quantification of p-MLKL relative to total MLKL. Necrotic cell death in the heart was determined by Evans blue staining assay. Upper panel: representative micro-photograph for necrotic cells in the heart; Bottom panel: quantification of necrotic cell death. and Echocardiographic analysis for myocardial function. Myocardial fibrosis was assessed by collagen deposition in the heart. Upper panel: representative micro-photograph for collagen deposition in the heart (red); Bottom panel: quantification of collagen deposition. and Biomarkers of myocardial injury: serum CK-MB ( ) and cardiac troponin I ( ). Data are meanSD, n=56. * <0.05 versus Sham+WT and <0.05 versus STZ+WT (ANOVA)",yes
PMC7649329,Figure_3,oa_package/e5/63/PMC7649329.tar.gz,['The soleus muscle of desmin knock-out mice shows a higher degree of fragmentation of their NMJs.'],"Figure 3 The soleus muscle of desmin knock-out mice shows a higher degree of fragmentation of their NMJs. The extensor digitorum longus or soleus muscle NMJs were categorized in groups according to their number of fragments. The number of NMJs in each group is given as the percentage of the total NMJ number for each genotype. For extensor digitorum longus a total of 96 wild-types and 89 homozygous, for soleus muscle 90 wild-type and 103 homozygous NMJs were counted ( = 3 mouse pairs). Mice were 34 months old. Statistical significance was determined by unpaired two-tailed -test (* < 0.05, *** < 0.001). The extensor digitorum longus or soleus muscle NMJs were categorized in groups according to their number of fragments. The number of NMJs in each group is given as the percentage of the total NMJ number for each genotype. For extensor digitorum longus a total of 102 wild-types and 95 homozygous, for soleus muscle 99 wild-type and 102 homozygous NMJs were counted ( = 3 mouse pairs). Mice were 78 months old. Statistical significance was determined by unpaired two-tailed -test (* < 0.05).",yes
PMC7553712,Figure_1,oa_package/f7/38/PMC7553712.tar.gz,"['While the moribund weight loss of 25% or greater seen in IFNAR-/- models was not observed, infected BALB/c mice lost 10% of their starting weight by 6 DPI, and never recovered to match uninfected animals (A).', 'These animals lost approximately 20% of their initial body weight, and were euthanized due to seizures at 3, 4, 5, 10, 11, and 12 DPI (B).', '.', '9 104 FFU/ml in these same tissues (C).', '9 104 FFU/ml (C).', 'To correlate live virus isolations to the amount of vRNA present within immune-privileged tissues, qRT-PCR was performed at 10 and 100 DPI in brain, eyes, and plasma in both immunocompetent and immunocompromised mice and showed that anti-IFNAR administration resulted in elevated viral load in all tissues (D).']","Figure 1. . (A) Body weight changes were monitored for 120 DPI. (N=10). PRVABC59 infected mice were immunocompetent, while mice denoted as IFNAR (anti-IFNAR) received a single administration of monoclonal antibody that functionally blocks IFNAR signaling 24hours prior to infection. The N for IFNAR mice decreased from 10 to 5 between 3 and 6 DPI, and further decreased from 5 to 1 between 10 and 30 DPI. These loses are denoted by vertical lines. The horizontal dotted line represents lethal weight loss endpoint. (B) Survival of mice infected with ZIKV with and without prior administration of IFNAR mAB. (C-D) Active viral replication was determined by infectious co-culture (C) at 0, 10, and 100 DPI. Total ZIKV genomic RNA was quantified by qRT-PCR (D; N =5) at 0, 10, and 100 DPI. For panels C and D, N =3 at 0 and 3 DPI, and for PRVABC59 group at 100 DPI. N =1 for IFNAR at 100 DPI. All error bars in all panels reflect Standard Deviation. Data was analyzed by Two-way ANOVA using Sidak post-hoc correction. For all panels, * is <.05, ** is <.01, *** is <.001, and **** is <.0001.",yes
PMC10734025,Figure_2,oa_package/11/ff/PMC10734025.tar.gz,"['Microglial cells challenged with oA 1 42 exhibited increased expression of cytokines TNF- ,\nIL-1 , and IL-6 ().', 'Conversely, our results showed that treatments with R-BCM\nfrom the three selected strains significantly reduced the level of\nIL-1 (A).', '30 Moreover, R-BCM from\nCRL 581 and CRL 2013 significantly reduced the level of expression\nof IL6 (B).', 'brevis CRL 2013, underscoring the effectiveness\nof R-BCM from both strains (C).', 'Postbiotics from selected LAB strains\nreduced the expression of\nIL-1 (A), IL-6 (B), and TNF- (C) in BV-2 cells stimulated\nby oA 1 42 5 M for 8 h.']","Figure 2 Postbiotics from selected LAB strainsreduced the expression ofIL-1 (A), IL-6 (B), and TNF- (C) in BV-2 cells stimulatedby oA 5 M for 8 h. Means for eachbar without a common letter differ significantly. Significance withTukeys HSD posthoc test following a one-way ANOVA is indicatedas < 0.05.",yes
PMC8465222,Figure_7,oa_package/72/a1/PMC8465222.tar.gz,"['Noteworthily, after 6 h, the plaque reduction was always above 93%, as reported in Table 6 and depicted in .', 'org/1999/xlink"" xlink:href=""pharmaceutics-13-01323-g006"" position=""float""/>Virucidal effect of SOL-MG (a), PG-MG (b), and PLO-3-MG (c) against the HSV-1 KOS strain (1 105 pfu/mL).']","Figure 7 Virucidal effect of SOL-MG ( ), PG-MG ( ), and PLO-3-MG ( ) against the HSV-1 KOS strain (1 10 pfu/mL). The virus was incubated for 1 and 6 h at 35 C with 50 mg/mL of MG loaded in the different gels ( , ) or in SOL-MG ( ). The data represent the percentage of viral growth after 1 or 6 h of incubation with the formulations. The values were obtained by comparing the results with those of the control. The data represent the mean of three independent experiments; * -values < 0.05.",yes
PMC3377938,Figure_1,oa_package/4a/54/PMC3377938.tar.gz,"['Graphical AbstractHighlights Mouse hepatitis virus ns2 protein inhibits the IFN-induced OAS-RNase L pathway ns2 reduces the intracellular level of 2 ,5 -oligoadenylate (2-5A) ns2 has a 2 ,5 -phosphodiesterase activity to directly cleave 2-5A ns2 mutant virus recovers wild-type virulence in RNase L-deficient micePublished: June 13, 2012IntroductionMany viral infections induce acute and/or chronic liver pathology in humans, for which there are often no known effective therapies or vaccines.', 'One potent antiviral IFN induced activity is the 2 ,5 -oligoadenylate synthetase-ribonuclease L (OAS-RNase L) pathway (Sadler and Williams, 2008) ( 1\n).', ' 1The Interferon-Induced OAS-RNase L PathwayAfter infection, viral RNA is detected by pattern recognition receptors RIG-I and MDA5, resulting in the induction of IFN- / , which in turn induces ISGs, including OAS.']",,yes
PMC2832107,Figure_3,oa_package/88/9f/PMC2832107.tar.gz,"['002""/>Ultrastructural characteristics of GRK2: immunopositive gold particles from the rat brain in control (same-operated: a, b) and 2-vessel occlusion (2-VO; resp.']","Figure 3 Ultrastructural characteristics of GRK2: immunopositive gold particles from the rat brain in control (same-operated: a, b) and 2-vessel occlusion (2-VO; resp., c, d) experiments. (a) Clusters of GRK2: immunopositive gold particles in the cytoplasmic matrix of perivascular pericytes (arrows) but not in the vascular EC, X 20,000. (b) The presence of GRK2 immunopositive gold particles associated with the edematous portion of the perivascular pericytes cytoplasmic matrix (arrows). Intact, but not giant, mitochondria (M) are free from any GRK2-immunopositive positive gold particles, X 30,000. (c) The GRK2 containing positive gold particles was seen in the hippocampal tissues from rat exposed to 2-VO. The presence of GRK2 immunopositive gold particles was seen throughout the matrix of damaged perivascular pericytes (arrows), X 8,000. (d) Perivascular regions of this area from figure (c) under higher magnification display the presence of islands of GRK2-containing gold particles which are associates with the damaged regions of the cytoplasmic matrix (arrows). Nucleus (N) and intact mitochondria are from the GRK2 immunoreactivity, X 30,000. (reprinted with permission of Neurotoxicity Research [ ]).",yes
PMC3757625,Figure_2,oa_package/b4/fd/PMC3757625.tar.gz,"['In case 4, the mass was localized to the pretarsal region without infiltration into the surrounding periorbital tissue [].']",Figure 2 Case four: (a) Note ptosis of right upper lid (b) CT scan showing diffuse mass involving right upper lid with areas of calcification (c) Intraoperative photograph showing amyloid in the pretarsal region,yes
PMC8425131,Figure_2,oa_package/86/f8/PMC8425131.tar.gz,"[' 2A, B).', 'A, B Fibroosseous proliferation in COF.', 'The osseous component is composed of acellular smoothly outlined rounded structures resembling cementum and present in a bland cellular fibrous stroma (H E, 40 and 100 magnification)A Irregular deposits of woven bone like material with intervening loose fibrovascular stroma and rimming of cementoblasts.']","Fig. 2 , Fibroosseous proliferation in COF. The osseous component is composed of acellular smoothly outlined rounded structures resembling cementum and present in a bland cellular fibrous stroma (H&E, 40 and 100 magnification)",yes
PMC7399600,Figure_11,oa_package/cf/a6/PMC7399600.tar.gz,[],Fig. 11 A 16-year-old boy following 1day of fever. An axial non-enhanced CT shows small rounded multifocal ground glass opacities bilaterally in the lower lobes associated with small vessels ( ),yes
PMC7693152,Figure_6,oa_package/53/35/PMC7693152.tar.gz,"['Indeed, for both diclofenac- and valproate-induced liver injury, moderate hypothermia could effectively sustain cell viability and avert cell death, comparable with uninjured cells in vitro ().', 'Coupled with its propensity to abate other DILI of similar pathology (), encompassing distinct characteristics of reactive metabolite formation alongside mitochondrial dysfunction and/or oxidative stress in diclofenac- and valproate-induced liver injury [35], it further fortifies the mechanisms of hypothermic action unraveled in this study.', 'Effect of moderate hypothermia (32 C) on diclofenac- and valproate-induced liver injury.']","Figure 6 Effect of moderate hypothermia (32 C) on diclofenac- and valproate-induced liver injury. L-02 cells are subjected to concomitant hypothermic conditioning and ( ) 2.5 mM diclofenac exposure or ( ) 10 mM sodium valproate exposure for 24 h. Cell viability was examined with cell titer-glo luminescent assay while cell death analysis was determined with propidium iodide staining. Data are presented as mean SD ( = 3), where unpaired -test was used to compare the effect of hypothermia on L-02 against their respective controls treated with the same drug dose. ** < 0.01, *** < 0.001.",yes
PMC10073857,Figure_3,oa_package/e3/37/PMC10073857.tar.gz,"['Delayed migration can be prevented by using partially covered stents and plastic stents within the metal stents (A C) and the use of both covered and uncovered stents to secure the intra-hepatic portion.', '34CONCLUSIONSThe advantages of EUS-BD over PTBD in MHBO with inaccessible papilla (D F) include lower patient discomfort and internal drainage distant from the tumor, thus lowering the risk of tissue ingrowth and stent block.', '.']","Fig. 3. Anchoring plastic stent placement within self-expanding metal stent (SEMS) during endoscopic ultrasound (EUS) guided hepatico-gastrostomy (HGS) (AC), combined EUS-guided gastrojejunostomy (D), and HGS for malignant obstruction with inaccessible papilla due to duodenal obstruction (E, F). (A) EUS-guided antegrade placement of guidewire across papilla. (B) SEMS placement through HGS fistula. (C) Anchoring plastic stent placement through SEMS. (D) EUS-guided gastrojejunostomy with a hot AXIOS stent. (E) EUS-guided antegrade guidewire passage for inaccessible papilla due to duodenal stenosis. (F) Post SEMS placement.",yes
PMC7414584,Figure_2,oa_package/6c/11/PMC7414584.tar.gz,"[' 2 summarized the pathological features of the control group.', 'Pathology findings of neural tissues and NMDAR staining in teratomas without anti-NMDARE.', '(n p) no immunostaining for NR1, NR2A, and NR2B in serial sections of the control caseImmunofluorescence staining revealed co-localization of NR1/NR2A/NR2B with IgG in neural tissue of teratomas.']","Fig. 2 Pathology findings of neural tissues and NMDAR staining in teratomas without anti-NMDARE. Pathology findings of neural tissues in teratomas and NMDAR staining from patients without anti-NMDARE. ( ) HE staining showed neurons with neuropil. ( ) a pitch of dysmorphic neurons with enlarged nuclei. ( ) Aggregates of neurons positive for MAP2. ( ) Neuroglia with moderate positivity for GFAP. ( ) Scattered cells with moderate positivity for S100. ( ) IgG positivity is moderate and spreading. ( ) Plasma cells positive for CD138. ( ) and ( ) Less positive for CD20 and clusters of lymphocytes positive for CD3. ( ) Helper T lymphocytes positive for CD4. ( ) Cytotoxic T lymphocytes positive for CD8. ( ) isotype controlof case C2. ( ) scattered dysmorphic neurons positive for NeuN. ( ) no immunostaining for NR1, NR2A, and NR2B in serial sections of the control case",yes
PMC11460918,Figure_3,oa_package/e4/ff/PMC11460918.tar.gz,['.'],Figure 3. The histopathological view of UCS with rhabdomyomatous differentiation (Black arrow) (case 3); (H&E 400).,yes
PMC11272914,Figure_7,oa_package/76/f4/PMC11272914.tar.gz,['Case 2: Histopathology(A-B) 10X and 40X magnification of dentinoid material surrounded by stellate reticulum-like cells with plump connective tissue cells.'],Figure 7 Case 2: Histopathology (A-B) 10X and 40X magnification of dentinoid material surrounded by stellate reticulum-like cells with plump connective tissue cells.,yes
PMC5630296,Figure_7,oa_package/e1/42/PMC5630296.tar.gz,"['CB2 agonist enhanced the production of paracrine growth factors and inhibited profibrotic cytokines in AD-MSCs(A-F) Paracrine growth factors including VEGF, bFGF, HGF, and IGF-1 as well as profibrotic cytokines including PDGF andTGF were examined by respective ELISA kits.']","Figure 7 CB2 agonist enhanced the production of paracrine growth factors and inhibited profibrotic cytokines in AD-MSCs ( ) Paracrine growth factors including VEGF, bFGF, HGF, and IGF-1 as well as profibrotic cytokines including PDGF andTGF were examined by respective ELISA kits. *P<0.05 between indicated groups..",yes
PMC5583981,Figure_6,oa_package/50/fa/PMC5583981.tar.gz,['Mitochondrial and neuritic network patterns are altered in frataxin-deficient sensory neurons.'],"Figure 6 Mitochondrial and neuritic network patterns are altered in frataxin-deficient sensory neurons. Representative images of cultured sensory neurons labeled with -tubulin III as neuronal marker (green) and Mitotracker (red) in basal culture of frataxin-deficient and control neurons and overloaded intra-axonal calcium control. The arrows indicate axonal spheroids and arrowheads the neuronal cell bodies. Magnification, 40 oil immersion. Scale bars, 50 m. Quantitative analysis of mitochondrial parameters: number of mitochondria; % of cellular area occupied by mitochondria; mitochondrial elongation; network interconnectivity; mitochondrial swelling; mitochondrial swelling distribution. The first 50 m proximal segments of axons were analyzed in control, YG8R, C57 treated with CCCP-oligomycin and C57 treated with A23 (185, 197, 59 and 99 total neurons measured respectively from at least three independent experiments). One way ANOVA (genotype) was used to analyze significant changes between genotypes and conditions. Significant -values: ** 0.01; *** 0.001.",yes
PMC8716544,Figure_1,oa_package/0e/87/PMC8716544.tar.gz,"['Ultrasound examination of the abdomen showed cystic liver lesions, ~38 28 and 13 8 mm in sizes, and separated light bands could be seen in the larger ones (A).', 'CT of the abdomen revealed multiple cysts in the liver; the larger ones were in the shape of a rosette and located in the lower part of the right lobe of the liver; the largest cross-sectional area was ~37 31 mm, and there seemed to be a separate structure inside (B).', 'In the arterial phase, the subcapsular enhancement focus of the lower right anterior lobe of the liver was enhanced (C), which is unclear in the portal phase (D) and cavernous hemangioma was considered.', '(A) Liver ultrasound.']",Figure 1 Liver ultrasound. Computed tomography (CT) plain scan. The arterial phase of enhanced CT scans. The portal phase of enhanced CT scans.,yes
PMC10403338,Figure_4,oa_package/a3/c8/PMC10403338.tar.gz,['(A) Coronal T1-weighted image demonstrates bilateral suprascapular and bilateral subscapular masses.'],Figure 4 (A) Coronal T1-weighted image demonstrates bilateral suprascapular and bilateral subscapular masses. The masses appear to contain alternating soft tissue and fatty components. (B) T1-weighted post-contrast image with fat saturation showing low-level enhancement.,yes
PMC11586248,Figure_2,oa_package/9c/84/PMC11586248.tar.gz,['Abdominopelvic computed tomography.'],Figure 2 Abdominopelvic computed tomography. The uterus is indicated by the white arrow.,yes
PMC3596603,Figure_1,oa_package/c0/ad/PMC3596603.tar.gz,[],"Fig.1 Microdissection, agarose-bead mediated preparation for PCR template and PCR amplification. Sections of the fundic gland polyp and the heterotopic gastric mucosa were selected strictly and subjected to microdissection under a light microscope. Schema of procedures for agarose-bead mediated preparation for PCR template. Microdissected samples were embedded in low-melting agarose. Beads were then formed by chilling in ice-cold water. The beads were treated with proteinase K, heat inactivated and subjected to PCR using a portion of the bead fragment as a template. PCR products were electrophoresed on a 2% agarose gel, stained with ethidium bromide and visualized under ultraviolet light. Lane M: a molecular weight marker of 100-bp ladder. The PCR products obtained using sets of primers encompassing exon 3 of the -catenin gene were visualized as 100-bp DNA bands. While the five cells microdissected from duodenum ( ) of case 1 does not show clear PCR product (case 1: , small), the rest of the samples containing at least 20 cells showed positive PCR amplification.",yes
PMC7665972,Figure_1,oa_package/5d/7b/PMC7665972.tar.gz,"[' 1, Table 2).', 'Immunoreactivity patterns of second-generation neuroendocrine markers in neuroendocrine neoplasms (NENs).', 'The fourth row illustrates the recurrent staining pattern in pheochromocytomas and paragangliomas, exemplified here by an abdominal paraganglioma with focal positivity for ISL1 and INSM1, while SECG was negativeINSM1 was noted with diffuse or focal nuclear positive immunoreactivity in 76/85 NENs (89%) and in 2/7 (29%) non-NENs with an initial suspicion of neuroendocrine differentiation (P = 0.', '1, Table 2).', '1, Table 2).']","Fig. 1 Immunoreactivity patterns of second-generation neuroendocrine markers in neuroendocrine neoplasms (NENs). All photomicrographs are magnified 400. First column are routine-processed slides stained with hematoxylin-eosin (H&E), followed by columns displaying immunoreactivity patterns for ISL1, INSM1, and secretagogin (SECG). The first row illustrates a surgically resected primary grade II Pan-NET (pancreatic neuroendocrine tumor) exhibiting positive ISL1, focally positive INSM1 (mixture of negative and positive nuclei), and positive SECG expression. The second row depicts a liver metastasis core needle biopsy of a Pan-NEC (pancreatic neuroendocrine carcinoma). Note the consistent immunoreactivity for all three markers. The third row exemplifies the typical staining patterns of a grade I small intestinal NET (SI-NET) with negative immunoreactivity towards ISL1, and focal INSM1 expression adjoined by a positive SECG staining. Note the normal intestinal mucosal layer to the left of each image, with scattered, normal neuroendocrine cells as internal positive controls. The fourth row illustrates the recurrent staining pattern in pheochromocytomas and paragangliomas, exemplified here by an abdominal paraganglioma with focal positivity for ISL1 and INSM1, while SECG was negative",yes
PMC4534050,Figure_3,oa_package/3e/e0/PMC4534050.tar.gz,"[' 3).', 'Non-ischaemic, non-HCM pattern of late gadolinium enhancement.', 'T1 mapping and ECV quantificationNative myocardial T1, equilibrium-contrast T1 and ECV values are shown in Table 5.']","Fig. 3 Non-ischaemic, non-HCM pattern of late gadolinium enhancement. Standard late gadolinium enhancement assessment using a fast low angle single shot inversion recovery sequence revealed non-ischaemic, non-HCM pattern of focal fibrosis in 13% of the hypertensive cohort, with LGE in the papillary muscles (A+B) mid-wall (B+C) and right ventricular insertion points ( ).",yes
PMC11292078,Figure_1,oa_package/27/4c/PMC11292078.tar.gz,"['The MLF is a paired white matter tract located near the midline of the brainstem ().', 'Neuroanatomy of MLF syndrome: the MLF is a white matter tract that interconnects the ocular cranial nerve nuclei (CNIII, CNIV, CNVI, and the EWN).']","Figure 1 Neuroanatomy of MLF syndrome: the MLF is a white matter tract that interconnects the ocular cranial nerve nuclei (CNIII, CNIV, CNVI, and the EWN). The manifestation of symptoms can vary based on the precise localization of the infarct within the MLF. In this case, the patient experienced an infarct (indicated by a red-white dot) near the pontomesencephalic junction, with preserved convergence eye movements. Figure was provided by and with the permission of Christopher Brown (Indiana University School of Medicine, Department of Neurological Surgery, Indianapolis, IN, USA). EWN, Edinger-Westphal nucleus; CNIII, oculomotor nerve; CNIV, trochlear nerve; MLF, medial longitudinal fasciculus; CNVI, abducens nerve.",yes
PMC3041045,Figure_2,oa_package/31/66/PMC3041045.tar.gz,"['(Multiple foci of skeletal malignant type FDG uptake with associated subtle sclerosis were seen on L1, L3, left sacrum left iliac bone, left anterior superior iliac spine, and subtrochanteric left femur ().', '.']","Figure 2. PET of the pelvis showing multiple foci of skeletal malignant-type FDG uptake, including left sacrum at the level of the S2 foramen and left iliac bone adjacent to the SI joint.",yes
PMC6836598,Figure_2,oa_package/47/7e/PMC6836598.tar.gz,"['At the 6-week postoperative follow-up, a well-defined anterior stromal scar was detected in the donor cornea upon slit lamp examination [a], which was subsequently confirmed by anterior segment OCT (AS-OCT) [', '(a) Slit lamp photography demonstrating stromal corneal haze.']",Figure 2 (a) Slit lamp photography demonstrating stromal corneal haze. (b) The same lesion demonstrated on anterior segment optical coherence tomography,yes
PMC3634033,Figure_7,oa_package/cc/dc/PMC3634033.tar.gz,"['Indeed, at the end of the culture period, STAT5 phosphorylation was clearly increased in PRL treated skin from three different individuals (A C), thus indicating functionality of PRLR-mediated signalling in human epidermis.', 'g007STAT5 phosphorylation is increased by PRL treatment in serum-free skin organ culture.']",10.1371/journal.pone.0060819.g007,yes
PMC8327930,Figure_2,oa_package/4f/94/PMC8327930.tar.gz,"[' illustrates a case where MRI prompted by discrepancy in size between initial imaging and WLE histology, revealed only expected inflammatory changes at the cavity but unsuspected high-grade DCIS distant from the surgical site altering management to completion mastectomy.', '.']","Figure 2. 51-year-old female with a symptomatic right breast mass. Mammogram (BI-RADS b, scattered fibroglandular density) showed a 35mm spiculate mass in the right upper outer quadrant (Figure 2a and b), measuring up to 19mm on ultrasound (Figure 2c) 14G core biopsy confirmed Grade 2 invasive NST. BCS was performed and histology showed a 60mm Grade 3 NST with adjacent satellite nodules. The discrepancy in size between imaging and histology prompted post-operative breast MRI which demonstrated a 9mm irregular enhancing mass in the right medial breast, distant from the site of surgery (Figure 3d). Second look ultrasound showed a corresponding 7mm ill-defined mass in the medial right breast (Figure 3e). Core needle biopsy showed high-grade DCIS, and the patient proceeded to completion mastectomy. Final histology confirmed high-grade DCIS measuring 14mm without invasive malignancy. BCS, breast conserving surgery;",yes
PMC10668929,Figure_7,oa_package/51/da/PMC10668929.tar.gz,"['The ability of US to assess fluid collections is especially useful in screening for pseudotumors in the setting of total hip arthroplasty (THA) or other hardware which may produce artifacts on CT or MRI ().', '0031_fig_007"" fig-type=""figure"">.']","Fig. 7. THA pseudotumor in a 60-year-old female with left hip swelling. Screening US was requested and long-axis US image of the left hip ( ) shows large fluid collection (*) with hyperemia on color Doppler interrogation at the anterior aspect of the left hip. Transverse CT image of the pelvis ( ) was requested the next day, confirming the pseudotumor (*). Note the streak artifacts emanating from the hardware on the CT image, though the large size of the pseudotumor enabled easy visualization",yes
PMC11021087,Figure_1,oa_package/57/f8/PMC11021087.tar.gz,"[':Preoperative magnetic resonance imaging of the brain revealed (a) T1 hypointense, (b) T2 hyperintense lesion in the left cerebellopontine angle cistern with (c and d) diffusion restriction and extensions into the ambient, quadrigeminal, and suprasellar cisterns with no postcontrast enhancement suggestive of epidermoid cyst.']","Figure 1: Preoperative magnetic resonance imaging of the brain revealed (a) T1 hypointense, (b) T2 hyperintense lesion in the left cerebellopontine angle cistern with (c and d) diffusion restriction and extensions into the ambient, quadrigeminal, and suprasellar cisterns with no postcontrast enhancement suggestive of epidermoid cyst.",yes
PMC11585470,Figure_3,oa_package/a3/db/PMC11585470.tar.gz,['CT noncontrast axial showing bilateral basal ganglia calcifications and right sided ACA/MCA infarcts.'],Fig. 3 CT noncontrast axial showing right ACA and MCA infarct.,yes
PMC9299925,Figure_1,oa_package/29/ba/PMC9299925.tar.gz,"['Clinical mapping was performed before, during, and after UVA 1 adjuvant phototherapy for cutaneous signs of eosinophilic fasciitis (a) and ultrasound mapping (b) for dermal, hypodermal, and fascial examination at established repere pointsUltrasound monitoringHigh frequency Ultrasound (HFUS) and ultra high Frequency Ultrasound (uHFUS) were realized with linear probes 22 MHz and 13 17 MHz (MyLab Twice Esaote biomedica ) and 55 70 MHz linear probe (VEVO MD \n, VisualSonics Fujifilm), respectively.']","Figure 1 Clinical mapping was performed before, during, and after UVA1 adjuvant phototherapy for cutaneous signs of eosinophilic fasciitis (a) and ultrasound mapping (b) for dermal, hypodermal, and fascial examination at established repere points",yes
PMC7683146,Figure_1,oa_package/a7/0f/PMC7683146.tar.gz,"['Magnetic resonance imaging (MRI) demonstrated a massive ventral spinal subdural hematoma from T12 to S1 ().', '0-00290933737561928T2-weighted MRI of the lumbar spine demonstrating ventral hypointensity relative to CSF, compressing the conus and cauda equina.']","Figure 1 T2-weighted MRI of the lumbar spine demonstrating ventral hypointensity relative to CSF, compressing the conus and cauda equina.",yes
PMC9289331,Figure_1,oa_package/b4/82/PMC9289331.tar.gz,"['In this observation we report a case of primary dural lymphoma in immune competent patient revealed by a intra cranial hypertension (see , , , , ).', 'CT-scan was performed objectifying a right frontal extra axial process with extension at the left frontal level intensively taken the contrast medium.', '']",Fig. 1 CT-scan was performed objectifying a right frontal extra axial process with extension at the left frontal level intensively taken the contrast medium.,yes
PMC4084682,Figure_5,oa_package/7c/0a/PMC4084682.tar.gz,"['We observed robust colocalization of DPP10789 with p-tau in most tangles and dystrophic neurites ().', 'In neurofibrillary tangles, DPP10789 and p-tau were fully colocalized (s 5(b), 5(e), and 5(g)).', 'Double staining using the DPP10789 antibody and Tau2 antibody (detects both phosphorylated and nonphosphorylated tau) also showed colocalization of DPP10789 and tau in neurofibrillary tangles and dystrophic neurites (s 5(a) and 5(c)).', '004""/>DPP10789 and tau colocalization in AD.']","Figure 5 DPP10 and tau colocalization in AD. Immunohistochemical colocalization of DPP10 with phosphorylated tau (p-tau) in tangles and plaque-associated dystrophic neurites. DPP10 (green) partially or fully colocalized with p-tau (red) in tangles (a, b, e, and g) and in plaque-associated dystrophic neurites (c, d). DPP10 was also present in intracellular vesicle-like structures (f) where very weak staining for p-tau was present. DPP10 immunoreactivity was also found in some tangles in the entorhinal region which were devoid of p-tau staining (h). DPP10 (green) did not colocalize with amyloid- plaques (red) in AD brain (i). Scale bar is 10 m. (a) and (c) used Tau2 ab and the remaining images are with AT8 ab.",yes
PMC9724674,Figure_5,oa_package/92/a5/PMC9724674.tar.gz,['DSS treated colons of PC deficient mice display elevated IL 6 signaling\nPreparation of conditioned medium.'],"Figure 4 PCdeficient mice are more susceptible to DSSinduced colitis Data information: (E, F, JM): * <0.05, ** <0.01, **** <10 by twotailed unpaired test; (N): * <0.05 by paired test. (C, E, F, JM). The box plot shows the median (inside line), 2575 percentiles (box bottom to top), and the Whiskers connect the minimum and the maximum values to the Box.",yes
PMC5552011,Figure_1,oa_package/24/30/PMC5552011.tar.gz,"['0 cm dense opacity in the right tonsillar fossa suggestive of a large tonsillolith (', 'This revealed a 3-cm fistulous tract extending from the right SMG to the right parapharyngeal space adjacent to the tonsillectomy site (', '']","Fig.1 Axial noncontrast neck computed tomography (CT) performed at an outside institution revealed a 17 10 12 mm calcification in the right tonsillar fossa (A, arrow) and a punctate calcification on the left (A, arrowhead). On the right, ductal distention in the right submandibular gland was not prospectively recognized to extend to the calcification (B and C, arrow). After right tonsillectomy and stone removal, the patient had continuing right submandibular swelling. Subsequent neck CT with contrast revealed increased size of the fluid tract from the right submandibular gland to the right parapharyngeal space (D, arrow), which was then excised.",yes
PMC7526536,Figure_4,oa_package/b0/0e/PMC7526536.tar.gz,"['Under all conditions, N-CAD was distributed continuously along cell junctions, whereas DSG2 localization was confined to punctuate stainings along the cell membrane ().', 'Treatment with a positive adhesiotropic mediator in combination with U0126 resulted in reduced DSG2 localization at the membrane, as compared with samples treated with the mediator alone ( and Supplemental ).', 'ERK1/2 inhibition caused alteration of DSG2 localization.']","Figure 4 ERK1/2 inhibition caused alteration of DSG2 localization. Immunostainings of HL-1 cells treated with F/R, PMA, or SB20, with and without U0126, stained for N-CAD and DSG2 under basal conditions. DMSO serves as control for SB20 treatment. Arrows indicate increased localization of DSG2 in the membrane. Scale bar: 10 m. = 6.",yes
PMC9670433,Figure_2,oa_package/e6/54/PMC9670433.tar.gz,"[' 2a), and a lobulated grayish-brown mass with a diameter of about 9 cm was observed in the posterior upper part of the prostate gland and seminal vesicle at the pelvic floor (', ' 2b).', ' 2c), and the postoperative pathological results were consistent with the diagnosis of splenosis (', 'a c Laparoscopic pelvic exploration: a multiple grayish-brown nodular tissues which was about 1.', '5 cm in diameter were observed in the pelvic cavity; b a lobulated grayish-brown mass with a diameter of about 9cm was observed in the posterior upper part of the prostate gland and seminal vesicle at the pelvic floor; 2c pelvic wound after mass excisiona, b Pathology: a rapid freezing of pathological results during the operation; b the postoperative pathological result (HE 40 )Discussion and conclusionsSplenosis occurs after rupture of the spleen or splenectomy [8], and its essence is heterotopic autologous splenic tissue implantation [2 4].']",Fig. 2 Laparoscopic pelvic exploration: multiple grayish-brown nodular tissues which was about 1.5 cm in diameter were observed in the pelvic cavity; a lobulated grayish-brown mass with a diameter of about 9cm was observed in the posterior upper part of the prostate gland and seminal vesicle at the pelvic floor; 2c pelvic wound after mass excision,yes
PMC9748654,Figure_5,oa_package/fd/63/PMC9748654.tar.gz,"['Immunohistochemical staining of inverted hyperplastic polyp showing the diffuse and strong cytoplasmic expression of glandular MUC-5AC in the polyp (EnVision; magnification, x200; scale bar=50 m).']","Figure 5 Immunohistochemical staining of inverted hyperplastic polyp showing the diffuse and strong cytoplasmic expression of glandular MUC-5AC in the polyp (EnVision; magnification, x200; scale bar=50 m).",yes
PMC4077262,Figure_1,oa_package/e8/5c/PMC4077262.tar.gz,['Histological tissue specimen separated by a thick capsule.'],Figure 1 (Hematoxylin and eosin stain; original magnification 40).,yes
PMC6999640,Figure_4,oa_package/61/cd/PMC6999640.tar.gz,"['Staining dendritic and axonal compartments using MAP2 and NEFH/NF200 respectively, we found a specific reduction in the axonal pool of ATG9A ((a,b); ATG9A vesicles per 10 m2; axonal: WT 5.', '1615302-F0004.', 'In KO axons however, we found a reduction in the number of motile LC3-positive structures ((c,d); RFP-LC3 structures; Total: WT 0.', '044; Mann-Whitney U), and that these nascent autophagosomes exhibited a reduced propensity to move retrogradely toward the soma, both in absolute retrograde displacement ((c,e); absolute retrograde displacement: WT 10.', 'A significantly reduced rate of LC3 lipidation was revealed in KO neurons, both basally, and with the induction of autophagy with rapamycin or EBSS ((j,k); Table S1).', 'Further, we found slowed SQSTM1/p62 accumulation in KO neurons during these treatments ((j,l); Table S1).', 'Interestingly, steady state levels of LC3 were unaffected in cultured KO neurons ((j,k)), but also in hippocampal lysates prepared from animals at 1 month ((m,n); relative LC3-II:I ratio: WT 1 0.', 'Further, we found a robust decrease in SQSTM1/p62 in KO animals at 1 month ((o,p); relative SQSTM1/p62 protein: WT 1 0.']",10.1080/15548627.2019.1615302-F0004,yes
PMC4882386,Figure_1,oa_package/ba/16/PMC4882386.tar.gz,"['One week later, control chest X-ray was performed again ().', 'Case 1.']",Figure 1 Case 1. Chest X-ray (A-P position). Circular shadow with a diameter of 26 mm is present in the place of previously described parenchymal changes (white arrows),yes
PMC7678286,Figure_3,oa_package/a6/e2/PMC7678286.tar.gz,"[' 3a, b, c).', ' 3d, e, f).', 'MR images after surgery: MR images 15 days after surgery (a, b, c).', 'The temporal lobe edema area has decreased remarkably on the T2WI imagesDiscussion and conclusionsWith a good long term tumor control rate and low morbidity, SRS offers a viable alternative for treating skull base meningioma [8, 9].']","Fig. 3 MR images after surgery: MR images15days after surgery ( , , ). MR images 6months after surgery ( , , ). The new lesion was completely removed, and the CSM achieved partial removal. The temporal lobe edema area has decreased remarkably on the T2WI images",yes
PMC7051910,Figure_5,oa_package/da/43/PMC7051910.tar.gz,"[' 5a) in the small intestinal tissue of infected mice.', ' 5b) and total cell numbers (', ' 5c).', ' 5d i), including the regulatory cytokine IL-10 by MLN CD4+ and CD8+ T cells (', ' 5d i).', ' 5a).', 'Batf2 deficiency drives heightened cellular inflammatory responses in the small intestine of S.', 'ns not significantDiscussionHere, we show that absence of Batf2 could dampen over-inflamed immunological state leading to resistance against tuberculosis and listeriosis in mice.']","Fig. 5 Batf2 deficiency drives heightened cellular inflammatory responses in the small intestine of mice. Control littermates (WT) and mice were percutaneously infected with 80 live cercariae and were killed at 8 weeks post infection. Concentrations of small intestinal cytokine levels normalized to mg of tissue were determined using ELISA. Flow cytometry was used to determine percentages ( ) and absolute numbers ( ) of CD4 intra-epithelial lymphocytes (IEL), CD8 IEL, CD4 CD8 IEL, CD8 dendritic cells, neutrophils (CD11b Ly6G ), macrophages (CD11b F4/80 ), and eosinophils (CD11b Siglec-F ), percentage of IFN- CD4 , IL-4 CD4 , IL-5 CD4 , IL-13 CD4 , IL-17 CD4 T cells in MLN. Percentage of IFN- CD4 , IL-4 CD8 , IL-5 CD8 , IL-13 CD8 , IL-17 CD8 T cells in MLN. Percentage and cell numbers of IL-4 CD4 , IL-5 CD4 , IL-13 CD4 , IL-17 CD4 IEL in small intestine. Percentage and cell numbers of IL-4 CD8 , IL-5 CD8 , IL-13 CD8 , IL-17 CD8 IEL in small intestine. Error bars denote meanSEM. Data shown are representative of one to three independent experiments with a sample size of =810 mice per group. * <0.05, ** <0.01, and *** <0.001 vs WT using one tailed Students -test. ns not significant",yes
PMC6145371,Figure_1,oa_package/7b/55/PMC6145371.tar.gz,"[' 1a for an illustration of the experimental design.', 'a Experimental design to examine the role of inflammagens on tau seeding activity.', 'c Representative fluorescent photomicrograph of Iba1 (red) and DAPI nuclear counterstain (blue) showing typical density and purity of mouse microglia cultures at 20Statistical analysisData was analyzed using GraphPad Prism 5.', ' 1b).', ' 1c).', ' 1), which is in line with previous research on cell-type specificity of tau gene expression [3, 55].', ' 1).', ' 1.']","Fig. 1 Experimental design to examine the role of inflammagens on tau seeding activity. Microglia or media alone was plated at DIV0. At DIV5, half of the media was replaced with fresh media containing rTg4510 lysate containing misfolded tau seeds () inflammagen of varying types (i), or media only (). Twenty-four hours later, media was collected for later application to the seeding assay. Gene expression of various cell type-specific transcripts was quantified by qPCR. Isolated microglia are enriched for the microglia marker CX3CR1 but not markers for astrocytes (Aldh1l1), neurons (Map2, MAPT), oligodendrocytes (MOG), or endothelial cells (PECAM1). Importantly, isolated microglia do not express the gene for tau, MAPT. Representative fluorescent photomicrograph of Iba1 (red) and DAPI nuclear counterstain (blue) showing typical density and purity of mouse microglia cultures at 20",yes
PMC7647565,Figure_3,oa_package/00/26/PMC7647565.tar.gz,['Histopathological results of the ovarian dysgerminoma and immunohistochemical staining.'],"Figure 3 Histopathological results of the ovarian dysgerminoma and immunohistochemical staining. After hematoxylin and eosin (H&E) staining, microscopic observation revealed that tumor cells were arranged in a nested shape, regular, round and quasi-circular with size uniformity. The cells had reduced cytoplasm, clear nuclear membrane, visible nucleolus and coarse chromatin, and karyokinesis was commonly observed. Necrosis could be observed in some areas, with fine fiber separation between the tumor nests, some focal lymphocytes infiltrated between the tumor cell mass and the interstitial space, and no adenoid and papillary regions(A). Immunohistochemistry revealed CD117, PLAP, OCT3/4 were all positive, and Ki-67 (about 90% +) (B, C, D and E respectively). Other indicators such as Vim, S100, CD30, AFP, inhibin-, CD99, CR (calretinin), and EMA were all negative.",yes
PMC8081915,Figure_2,oa_package/0f/e7/PMC8081915.tar.gz,['CT scan with IV contrast demonstrated marked diffuse dilatation of small bowel loop and the cecum with abrupt point of transition at the junction of cecum and the ascending colon.'],Fig. 2 X-ray chest erect.,yes
PMC8611923,Figure_6,oa_package/e9/cb/PMC8611923.tar.gz,"[' 6) and left anterior tibia skin (', '55)ANANegativeHepatitis B surface AntigenNegativeHepatitis A antibodyNegativeHepatitis C antibodyNegativeHepatitis E antibodyNegativeHuman immunodefciency virus antibodyNegativeAnti-smooth muscle antibodiesNegativeAnti-liver and kidney microsome antibodiesNegativeAnti-mitochondrial antibodiesNegativeAnti-myocardial antibodiesNegativeAnti-parietal cell antibodiesNegativeAnti-neutrophil cytoplasmic antibodiesNegativeRight parotid gland plasmacyte IgG4 positivity with immunohistochemistry']",Fig. 6 Right parotid gland plasmacyte IgG4 positivity with immunohistochemistry,yes
PMC2526176,Figure_2,oa_package/86/61/PMC2526176.tar.gz,"['A population of endothelial cells remained intact following HV68 infection and uniformly expressed lytic antigensUninfected endothelial cells and fibroblasts grew as adherent monolayers (A, top panel).', 'The cells harvested from the infected endothelial cultures appeared as individual, intact cells suspended in culture, whereas the material harvested from the infected fibroblast cultures appeared as clumps of cellular debris (A, bottom panel).', 'Very few infected fibroblasts remained as viable, non-adherent cells at six days post-infection (B).', 'When MB114 endothelial cells were treated with a non-toxic dose of phosphonoacetic acid (PAA), an inhibitor of viral DNA replication and late gene synthesis, most cells remained adherent, and there were significantly fewer detached, viable cells (B).', 'g002A population of endothelial cells remained intact following HV68 infection.', 'The majority of MB114 endothelial cells and 3T12 fibroblasts were positive for surface gp150 expression (C).', '3 SEM) of the cells harvested were intact and viable (D).', 'The observed increase in both intact cell number and percent viability during the first six days of culture, concurrent with positive gp150 staining (C and ', 'Of significance, during the course of this infection most of the cells expressed the lytic glycoprotein gp150 on their surface at both early and late time points (C and ', 'Moreover, the percentage of cells expressing gp150 did not change markedly with time (C and ', 'PAA treatment immediately following MB114 infection resulted in significantly fewer cells becoming non-adherent (B).', 'Our analysis of endothelial cell infection in the presence of PAA indicated that this outcome is influenced by viral DNA replication and/or late gene synthesis (B and ', 'Our in vitro studies revealed that viable, infected endothelial cells were not latently infected, but uniformly supported a lytic viral program (C, ', 'Because viral DNA replication and/or late gene synthesis were important for endothelial cell outcome of HV68 infection (B and ']",10.1371/journal.ppat.1000152.g002,yes
PMC10353146,Figure_1,oa_package/e9/28/PMC10353146.tar.gz,"[' 1).', 'Schematic of gastric cardiac cancer (GCC).', 'B White-light endoscopy image of GCCTo help predict survival outcomes and make treatment decisions, the American Joint Committee on Cancer (AJCC) staging system has been developed and widely used to classify patients based on tumor, node, and metastasis (TNM) stage [12].', 'tiff"">Additional file 1: Supplement .']",Fig. 1 Schematic of gastric cardiac cancer (GCC). Location of GCC. White-light endoscopy image of GCC,yes
PMC9719013,Figure_7,oa_package/42/e2/PMC9719013.tar.gz,"['Microscopically, the ovarian nodule was composed of numerous small vascular spaces, consistent with ovarian hemangioma, capillary-type (H E stain, 40 ).', 'Higher magnification shows the vascular spaces to be lined by a single of endothelial cells and containing red blood cells (H E stain, 100 ).']","Fig. 7 Microscopically, the ovarian nodule was composed of numerous small vascular spaces, consistent with ovarian hemangioma, capillary-type (H&E stain, 40).",yes
PMC8407264,Figure_2,oa_package/1f/99/PMC8407264.tar.gz,"['The red vertical line in corresponds to the ultrasound beam penetration in the skin.', 'The white amplitude curve on the blue background seen in is an example of A-scan.', 'Subcutaneous fatTwo-dimensional 75 MHz HFUS scan and one-dimensional A-scan.', 'Superficial fascia presents intact dorsal palmar skin 75 MHz scan of a 34-year-old healthy person.', 'As it can be seen in , there is a color scale above the A-scan diagram used to convert amplitude values to adequate pixel color.']","Fig. 2 Two-dimensional 75 MHz HFUS scan and one-dimensional A-scan. Red vertical line corresponds to the direction of penetration and the reflection of ultrasonic wave. 1. Epidermis, 2. Dermis, 3. Lower dermis, with border between dermis and subcutaneous fat, 4. Subcutaneous fat, 5. Superficial fascia",yes
PMC10628574,Figure_3,oa_package/35/75/PMC10628574.tar.gz,['Transverse axial scan of the arterial phase.'],"Figure 3 Transverse axial scan of the arterial phase. The lesions showed uneven enhancement, the highest CT value reached 192HU, and the low-density areas were not enhanced.",yes
PMC6062601,Figure_1,oa_package/ed/c3/PMC6062601.tar.gz,[],"FIGURE 1 PreM group showed increased lipid concentration and fat accumulation in liver after transplantation for 4 weeks; Representative photomicrographs of hematoxylin- and eosin-stained sections of the liver (100 magnification); bar graph of the volume density of liver steatosis; hepatic triglyceride; hepatic total cholesterol; serum leptin; serum LBP; and serum SAA levels. Data are shown as the mean SEM and were compared between groups and time points using the MannWhitney test; < 0.05. = 4 or 5 for each group. LBP, lipopolysaccharide binding protein; SAA, serum amyloid A protein.",yes
PMC4899798,Figure_4,oa_package/53/5d/PMC4899798.tar.gz,"['As shown in A, the percentage of pronephric kidney abnormalities (mild and severe) was significantly increased in lipl32 mRNA-injected larvae compared with control (30/35 vs 3/30, P 0.', ' The expression of l-plastin was markedly increased in lipl32 mRNA-injected larvae and the staining was merged to the wt1b:GFP signal (B), suggesting that these inflammatory cells infiltrated the pronephric kidneys.', 'As illustrated in C, the expression of LipL32 disoriented the basolateral location of NA-K-ATPase to the apical membrane of the renal epithelial cells.', 'In contrast, immunostaining for the primary cilia, which is responsible for the mechanosensation of renal tubular epithelium, did not show any structural abnormality in lipl32 mRNA-injected larvae (D).', 'We also found that the glomerular filtration rates, as estimated by the excretion of injected 10-kDa rhodamine dextran, were significantly reduced in the lipl32 mRNA-injected larvae compared to controls (E), indicating a functional impairment of the affected pronephric kidneys.', 'org/1999/xlink"" xlink:href=""srep27838-f3""/>LipL32 induced pronephric malformation, inflammatory cell infiltration and translocation of NA-K-ATPase in zebrafish tubular epithelial cells.']","Figure 4 LipL32 induced pronephric malformation, inflammatory cell infiltration and translocation of NA-K-ATPase in zebrafish tubular epithelial cells. ( ) Quantification of normal, mild or severe deformities of pronephros between groups of :GFP larvae at 48hpf (n=30 to 35 in each group). Photos were collected by observation under fluorescence microscopy (dorsal view, anterior to the left). Pronephric kidneys show abnormalities in glomerular fusion, cystic changes, and deformities of pronephric tubules and ducts. Scale bar, 50m. ( ) hybridization shows that positive cells (pseudo-colored by red) were increased in mRNA-injected larvae compared to control. Arrows indicate a colocalization (orange) of GFP staining (green) and in the pronephric tubule and duct. Scale bar, 50m. ( ) Immunostaining for NA-K-ATPase shows the normal basolateral location of NA-K-ATPase in the pronephric ducts was disorganized in mRNA injected larvae (scale bar, 10m). Arrows indicate the basolateral cell surface. Right panels are transverse sections on whole-mount stained larvae on the left (scale bar, 2m). A diagram illustrating the changes in the cellular location of NA-K-ATPase is shown. ( ) Immunostaining for acetyl-tubulin shows no significant differences of pronephric cilia (arrows) between groups (scale bar, left panel, 10m, right panel, 2m). ( ) The retention rates of 10-kDa rhodamine dextran as measured from the boxed region of the posterior pronephric ducts 6 hours after pericardial injection were significantly reduced in the mRNA-injected larvae compared to -mRNA controls (** <0.01, n=10 to 11 in each group, scale bar, 100m). Transverse sections demonstrate retention of rhodamine fluorescence in the lumen of pronephric ducts (circle) in mRNA-injected larvae (scale bar, 5m).",yes
PMC4422728,Figure_5,oa_package/eb/97/PMC4422728.tar.gz,"['The levels of A proteins were separately measured in the water-soluble (A), detergent-soluble (B), and detergent-insoluble (C) fractions.', ', 21 and 24 months of age), this relationship between A 40 and A 42 was reversed (A); in addition, in 21- and 24-month-old mice with high plaque loads, levels of A 42 decreased from water-soluble to detergent-soluble and insoluble fractions, whereas levels of A 40 increased (Fig 14] was used to extract brain proteins that were used: 1) to measure levels of total A in rTg9191 mice (), 2) to measure levels of water-soluble A dimers in rTg9191 mice (Fig LS clearly visualizes the relationship between lung cancer (indicated by the arrows) and the surrounding obstructive pneumonia.']",Figure 4 LS clearly visualizes the relationship between lung cancer (indicated by the arrows) and the surrounding obstructive pneumonia.,yes
PMC9781645,Figure_7,oa_package/a0/2b/PMC9781645.tar.gz,"['HE staining demonstrated the phrenic nerve and esophagus cell boundaries were clear and neatly arranged without any damage ().', 'Hematoxylin and Eosin (E A) staining.']",Figure 7 Hematoxylin and Eosin (E&A) staining. ( ) Represent the phrenic nerve. ( ) Represent the esophagus. Note that the cell boundaries are clear and neatly arranged without any damage.,yes
PMC3894444,Figure_1,oa_package/fa/30/PMC3894444.tar.gz,"['The mass showed heterogeneous low attenuation on unenhanced CT, and there was no evidence of internal calcification or fat (A).', 'After administration of intravenous contrast media, multifocal patchy irregular enhancement in the peripheral area was seen during the arterial phase (B).', 'During portal venous and delayed phases, patchy progressive centripetal enhancement was noted (C, D).', 'Sclerosing liver haemangioma with pericapillary smooth muscle proliferation: atypical CT and MR findings with pathological correlationBr J Radiol200881e162e165184873828YamashitaYShimadaMTaguchiKGionTHasegawaHUtsunomiyaTHepatic sclerosing hemangioma mimicking a metastatic liver tumor: report of a caseSurg Today200030849852110397189LeeVTMagnayeMTanHWThngCHOoiLLSclerosing haemangioma mimicking hepatocellular carcinomaSingapore Med J2005461401431573588010MathieuDRahmouniAVasileNJazaerliNDuvouxCTranVJSclerosed liver hemangioma mimicking malignant tumor at MR imaging: pathologic correlationJ Magn Reson Imaging19944506508806145511MiettinenFletcherCDMKindblomLGZimmermannATsuiWMSMesenchymal tumours of the liverBosmanFTCarneiroFatimaHrubanRHTheiseNDWHO classification of tumours of the digestive systemLyon, FranceIARC Press2010241250Contrast-enhanced abdominal CT images of a 64-year-old woman with a sclerosing hemangioma of the liver.']","Figure 1 Contrast-enhanced abdominal CT images of a 64-year-old woman with a sclerosing hemangioma of the liver. A precontrast CT scan revealed a large irregular shaped mass with heterogeneous low attenuation in the left lobe of the liver (arrows) (A). Arterial (B), portal (C) and delayed (D) phases of CT scans revealed heterogeneous enhancement in the peripheral area of the mass (arrows) with a gradual centripetal enhancement pattern except for a central area of persistent low attenuation corresponding to sclerosis by histopathology (arrow head).",yes
PMC7554859,Figure_9,oa_package/11/3e/PMC7554859.tar.gz,"[' shows a discontinuity in the vascular wall of a kidney vessel in the mid-medulla region after in vivo administration of Iopromide.', 'However, the SEM examination, as a high-resolution method, revealed a slight activation of the plasmatic coagulation in some kidney samples ( and 0).', 'Scarce fibrin fibers () and few mural thrombi (0) were only found in Iopromide-exposed kidney vessels in SEM analyses.', 'Vascular wall discontinuity (see arrow) of a kidney blood vessel in the middle medulla sealed with cascades of adhering platelets (see asterisk) in a mesh of thicker well-organized fibrin fibers (platelet thrombus), adjoining erythrocytes (intravascular), and loosely aggregated platelets with scarce fibrin constituents in the surrounding area (extravascular) after in vivo administration of Iopromide.']","Figure 9 Vascular wall discontinuity (see arrow) of a kidney blood vessel in the middle medulla sealed with cascades of adhering platelets (see asterisk) in a mesh of thicker well-organized fibrin fibers (platelet thrombus), adjoining erythrocytes (intravascular), and loosely aggregated platelets with scarce fibrin constituents in the surrounding area (extravascular) after in vivo administration of Iopromide.",yes
PMC2938495,Figure_15,oa_package/1b/22/PMC2938495.tar.gz,[],Figure 15 M-mode of asystole with absence of cardiac contractions,yes
PMC9713829,Figure_5,oa_package/d0/07/PMC9713829.tar.gz,"['Eosinophilic secretions were seen inside the tubules ().', '.']","Figure 5. Sample of tumor. Small, scattered tubular structures, lined with transitional cells with amphiphilic or clear cytoplasm, with eosinophilic secretions inside the tubules. Hematoxylin-eosin staining (magnification, 100).",yes
PMC11404607,Figure_1,oa_package/e7/bf/PMC11404607.tar.gz,"['In contrast to the systemic infection where the absence of YjbH enhanced pathogenesis, in the skin model, the yjbIH mutant had significantly smaller surface lesions (Supplementary ).', 'This was true for the necrotic scab-like portion of the lesion (a,c) and the total lesion (Supplementary ), which is the largest surface area that includes both blanching of the skin and the necrosis.', 'At times, no surface lesion was apparent in yjbIH mutant-infected mice, but the infection appeared as a subdermal abscess (Supplementary ).', 'The decreased lesion size observed by the yjbIH mutant could be restored with yjbIH provided on a plasmid (c, Supplementary s 1, and 2a).', 'The reduced lesion size was not the result of decreased colonization as there was no difference in bacterial titers at the site of infection (b,d).', 'YjbI had no discernable impact on lesion formation (a, Supplementary s 1, 2c, and 3); however, the yjbH mutant phenocopied the yjbIH mutant with lower total lesion (Supplementary b) and necrosis (a,c).', 'This mutant often presented as a subdermal abscess as well (Suplementary ).', 'We observed a modest reduction in bacterial titers at the site of infection for the yjbH mutant (f).', 'As with yjbIH, the yjbH mutant phenotype could be restored when yjbH was provided on a plasmid (e,f and Supplementary 2b).', '.', 'Homogenized infected skin from mice in e was analyzed by cytometric bead arrays.', 'Controlled expression of hla alleviates YjbH effects on tissue damageSince the yjbH mutant has reduced lesion formation during skin infection and reduced Hla activity ( and 3), we predicted that if we expressed Hla from a non-native promoter, tissue damage during infection would be unaffected by the absence of YjbH.', '( and 6).']","Figure 1. YjbH contributes to tissue damage during SSTI. C57BL/6J were infected subcutaneously with wild type (WT), mutants in , , , and respective complement plasmids as indicated. (a,c,e) Shown as necrosis size over time. Symbols represent the mean ( =5-8) with SEM. (b,d,f) bacterial titers enumerated on day four post-infection. Bars represent the mean with SEM. Each dot represents an individual mouse. Data is representative of at least three independent experiments. *, <0.05; **, <0.01 by two-tailed Mann-Whitney test compared to WT.",yes
PMC9510869,Figure_2,oa_package/6a/35/PMC9510869.tar.gz,"['Static images were recorded with the cords abducted ( 2, A) and adducted ( 2, B).', ' 2Static images demonstrating cords abducted (A) and cords adducted (B) on laryngeal ultrasound.']",,yes
PMC2803955,Figure_2,oa_package/97/93/PMC2803955.tar.gz,['Low magnification ( 10) revealed one cyst in the thickness of the dermis composed of 2 layers of cuboidal cells (Hematoxiline eosine).'],Figure 2 .,yes
PMC5596674,Figure_1,oa_package/d2/61/PMC5596674.tar.gz,['Clinical and radiographic features of glandular odontogenic cyst.'],Figure 1 Clinical and radiographic features of glandular odontogenic cyst. (a and b) Extraoral and intraoral photograph of Case #4 showing bony hard swelling in anterior mandible. (c) Occlusal radiograph of Case #4 showing well-defined unilocular radiolucency. (d) OPG of Case #2 showing well-defined multilocular radiolucency in the mandible. (e) CT scan of Case #3 showing expansile lytic multilocular lesion in the anterior maxilla,yes
PMC9411008,Figure_4,oa_package/83/15/PMC9411008.tar.gz,"['Histopathological results also showed an adenocarcinoma with moderate differentiation, which was identical to the primary colon cancer ().', '003"" position=""float""/>Histological findings of resected testis showed an adenocarcinoma with moderate differentiation, identical to the primary colon cancer (H E stain, 200).']","Figure 4 Histological findings of resected testis showed an adenocarcinoma with moderate differentiation, identical to the primary colon cancer (H&E stain, 200).",yes
PMC3764950,Figure_1,oa_package/9f/47/PMC3764950.tar.gz,[' Phrygian cap gallbladderCan J Surg2003465051125857955LamahMKaranjiaNDDicksonGHAnatomical variations of the extrahepatic biliary tree: review of the world literatureClin Anat200114167172113014626EdellSA comparison of the Phrygian cap deformity with bistable and gray scale ultrasoundJ Clin Ultrasound1978634354160457DalalSChauhanTSKumarRChoudhurySRPseudo-duplication of the gall bladder due to Phrygian cap a case reportInternet J Surg2013298BoydenEAThe Phrygian cap in cholecystography: a congenital anomaly of the gallbladderAm J Radiol1935335899MeilstrupJWHopperKDThimeGAImaging of gallbladder variantsAJR Am J Roentgenol199115712051208195086710SmergelEMMaurerAHPhrygian cap simulating mass lesion in hepatobiliary scintigraphyClin Nucl Med198491311336538468Perioperative photograph of the gallbladder.'],Fig. 1 Perioperative photograph of the gallbladder. The fundus of the gallbladder is folded like a cap (arrow).,yes
PMC9495364,Figure_8,oa_package/6a/79/PMC9495364.tar.gz,"['Instead, shows the final detection results on the validation datasets V1 and V4 with the NDG-CAM method, the Mask R-CNN architecture, and the combined adoption of both methods.', 'The two methods show similar performance on the V1 dataset, as can be observed from .', 'Examples of centroid detection on the validation sets V1 and V4.']","Figure 8 Examples of centroid detection on the validation sets V1 and V4. ( ) Green, NDG-CAM method detections; red, Mask R-CNN detections. ( ) Blue, combined method detections. First and second columns show data from V4, whereas the third and fourth columns depict data from V1.",yes
PMC3669386,Figure_2,oa_package/29/b7/PMC3669386.tar.gz,"['Representative images obtained by echocardiography in short axis view of a two-dimensionally directed M-mode at d35 are shown in .', 'g002Representative images obtained by echocardiography at day 35 in Ctrl (A) and Dox-CM (B) rats.']",10.1371/journal.pone.0064711.g002,yes
PMC4118420,Figure_3,oa_package/e5/1b/PMC4118420.tar.gz,"['These suggest that tiny calcium precipitates are caused by a different mechanism from that of vascular calcification and so we discussed possible mechanism in the figure legend of supplementary .', ' CT imaging clearly showed the characteristic bone phenotypes of -kl-/- mice, such as a generalized decrease in bone mineral density, significantly reduced trabecular and cortical thickness, lower trabecular and cortical bone volume and a severely reduced trabecular bone fraction (b, e, i, j, n).', 'All these phenotypes were improved closed to wild-type levels by BDA-410 administration (c, f, k, l, o).', 'Furthermore, malformation of spongy bone (n), structural deformities of bones and irregular/rough appearances of bones (', '3e, blue arrows) seen in -kl-/- mice were ameliorated by BDA-410 administration (o, f).', 'Ectopic calcification in the aorta of -kl-/- mice (e, p; yellow arrows) was no longer detectable in BDA-410-treated animals (', 'org/1999/xlink"" xlink:href=""srep05847-f2""/> CT imaging of bones.']","Figure 3 CT imaging of bones. Whole-body mouse CT images were obtained from wild type and mice (N = 2 each) which were treated with vehicle or BDA-410, respectively. Whole-body bones of wild-type (BDA-410) (a), (Vehicle) (b), and (BDA-410) (c). Spinal columns of wild-type (BDA-410) (d), (Vehicle) (e), and (BDA-410) (f). Tibiae of wild-type (BDA-410) (g, h), (Vehicle) (i, j), and (BDA-410) (k, l). Lumbar 1s of wild-type (BDA-410) (m), (Vehicle) (n), and (BDA-410) (o). Yellow arrows indicate ectopic calcifications in the aorta (e, p). Small calcium depositions enclosed by red circles are derived from fish bones that were provided in the diet. CT imaging pictures of bones of vehicle treated wild-type mice are shown in .",yes
PMC10026180,Figure_5,oa_package/9d/3a/PMC10026180.tar.gz,"['Widespread ulceration on the buttocks and scrotum with a central black eschar in their center and yellow-green exudate in a 5-month-old girl with DOCK8 deficiency, consistent with Candida and Pseudomonas infection.']","Figure 5 Widespread ulceration on the buttocks and scrotum with a central black eschar in their center and yellow-green exudate in a 5-month-old girl with DOCK8 deficiency, consistent with and infection.",yes
PMC6906425,Figure_10,oa_package/2f/64/PMC6906425.tar.gz,[],Figure 10 Immunohistochemical staining against TRPML1 in symptomatic degenerated IVD sections with high TRPML1 staining intensity in ( obtained from patients suffering from intense low back pain (n.37 and n.39 respectively) as compared to sections with moderate staining intensity ( obtained from patients suffering from disabling low back pain (n.36 and n.38 respectively); and sections negative for TRPML1: ( ) asymptomatic (non-painful) IVD section with mild multifocal degenerative changes (n.48) and non-degenerated IVD (n.49). Scale bar is 100m.,yes
PMC8303262,Figure_6,oa_package/d1/fc/PMC8303262.tar.gz,"['Representative immunohistochemical images utilizing the AT8 antibody (a f) and quantifications demonstrated that p-tau had overall decreased in the hippocampus and fornix in 3xTg-AD iPSC-NPCs mice compared to age-matched 3xTg-AD PBS mice (n = 7/group; FC: 1.', '05) (g).', '3xTg-AD mice transplanted with iPSC-NPCs show a reduction in tau pathology.']","Figure 6 3xTg-AD mice transplanted with iPSC-NPCs show a reduction in tau pathology. We analyzed tau hyperphosphorylation by using a phospho-specific anti-tau antibody, AT8. Tau immunostaining was decreased in the hippocampus and fornix of 3xTg-AD mice injected with iPSC-NPCs ( , , ) compared with PBS-injected 3xTg-AD mice ( , , ). AT8 was counterstained with hematoxylin ( ). Scale bar: ( , ) 300 m; ( ) 25 m. ( ) Image analysis of AT8 p-tau burden in the hippocampus revealed lowered levels in 3xTg iPSC-NPCs mice versus 3xTg-AD PBS mice ( = 78/group) (student -test, * < 0.05). Data corresponds to the means SEM.",yes
PMC11297113,Figure_5,oa_package/0e/88/PMC11297113.tar.gz,['The bone window of the larynx brought up a left-sided undislocated fracture of the cricoid cartilage with buckling (arrows in C and D)Axial and paracoronal NECT scan; bone window with a ST of 2 mm.'],Fig. 5 Axial and coronal NECT; soft tissue (3mm ST) and bone window (axial and paracoronal) with a ST of 2mm. The axial ( ) and coronal ( ) NECT images demonstrate slight edema and stranding of the subcutaneous fat in the right submental region with slight edematous thickening of the cutis (arrows). The bone window of the larynx brought up a left-sided undislocated fracture of the cricoid cartilage with buckling (arrows in and ),yes
PMC3107228,Figure_6,oa_package/57/22/PMC3107228.tar.gz,"['Upon treatment with PolyIC for 48 h, 1IFNR / DC lines were protected from PolyIC-induced apoptosis (\nA and B\n), in agreement with the requirement for the 1IFNR for splenic cDC loss in vivo (\n', 'Strikingly, TLR3 / DC lines were also resistant to PolyIC, whereas MAVS / DC lines remained normally susceptible (\nA and B\n).', 'Importantly, MAVS / DC lines produced IFN in response to PolyIC, whereas the resistance of TLR3 / DC lines correlated with a lack of IFN production (\nC\n), in agreement with the requirement of TLR3 for IFN production by freshly isolated splenic CD8 cDC (\n', 'g006DC lines show TLR3-dependent IFN secretion with PolyIC and sufficiency of IFN for apoptosis induction.', 'Notably, except for 1IFNR / DC lines, all other DC lines readily underwent apoptosis after 48 h of treatment with IFN , including the PolyIC-resistant TLR3 / DC lines (\nA and B\n).', 'In addition, effector caspase-3/-7 and initiator caspase-8 activities were clearly evident in WT DC lines after 30 h of stimulation with either PolyIC or IFN (\nD\n).']",10.1371/journal.pone.0020189.g006,yes
PMC10758170,Figure_1,oa_package/cb/96/PMC10758170.tar.gz,"[' CT and MRI scan showing hyperintense perilesional edemaA and D are selective images of an unenhanced CT scan of the brain at the level of the centrum semiovale and posterior horn of the right lateral ventricle, respectively.']","Figure 1 CT and MRI scan showing hyperintense perilesional edema A and D are selective images of an unenhanced CT scan of the brain at the level of the centrum semiovale and posterior horn of the right lateral ventricle, respectively. They exhibit an iso- to hyper-dense, well-defined lesion with surrounding vasogenic edema (arrow) within the right parietal lobe. B and E are T2-weighted images at the same mentioned levels, displaying a hypointense lesion superiorly and hyperintense content inferiorly with a hypointense rim (arrowhead on E) and surrounding hyperintense vasogenic edema (arrow). C and F show the same lesion on T1-weighted images without contrast, demonstrating a gradient of hyperintense content with a hypointense rim and hyperintense perilesional edema (arrow).",yes
PMC9550486,Figure_4,oa_package/ed/22/PMC9550486.tar.gz,"['The skin changes secondary to bile staining completely resolved ().', '003"" position=""float""/>Right-sided parastomal hernia 2-month after discharge.']",Figure 4 Right-sided parastomal hernia 2-month after discharge. Bile staining skin changes completely resolved. Superior peristomal skin ulceration.,yes
PMC4674771,Figure_2,oa_package/42/c4/PMC4674771.tar.gz,"['035, a).', '029, b), and with local measures of AD pathology in the temporal cortex, namely A (n = 90, r = 0.', '020, c) and tau area immunoreactivity (n = 89, r = 0.', '022, d).', 'GS expression associates with Alzheimer-type pathology.']","Fig. 2 GS expression associates with Alzheimer-type pathology. Increasing GS astrocytes associated with (a) FOXO3a glia, (b) Braak stage, (c) -amyloid plaques and (d) levels of tau (AT8 immunoreactivity).",yes
PMC11592716,Figure_5,oa_package/91/45/PMC11592716.tar.gz,"['Feature maps of significantly different features in the peripheral zone are shown in .', 'Exemplary feature maps of best-performing features in the peripheral zone.']","Figure 5 Exemplary feature maps of best-performing features in the peripheral zone. In the upper row, images of a patient with clinically insignificant prostate cancer (ciPCa, Gleason Score 3 + 3, prostate-specific antigen (PSA) density: 0.17 (ng/mL)/cm ) and in the lower row images of a patient with clinically significant prostate cancer (csPCa, Gleason Score 3 + 4, PSA density: 0.17 (ng/mL)/cm ) are shown. On the left, the original T2-weighted axial image and the segmented target lesion marked with a volume of interest (VOI, yellow) are displayed. On the right, the parametric feature maps of the first-order median, first-order total energy, and glcm inverse difference normalized are shown. The map-derived features first-order median and first-order total energy revealed excellent diagnostic performance in differentiating csPCa from non-csPCa (no PCa and clinically insignificant PCa). VOIs were copied based on software in the maps, as shown for the glcm inverse difference normalized map, to extract feature quantity directly.",yes
PMC10469006,Figure_5,oa_package/5d/45/PMC10469006.tar.gz,[],"Figure5 Effect of AN1284 on hepatic immune cells, anti-inflammatory cytokines, and CCL2 in mice on a WD. . Immunohistochemical staining of immune cells in mice livers on a WD. CD3 for T cells, F4/80 for macrophages, CD45R for B cells, and Ly6B for neutrophils and NK cells. Calibration bar, 20 M. . mRNA levels of CCL2 in the liver of mice on a WD. WD increases T cells, macrophages and CCL2 expression. AN1284 (1 mg/kg/day) significantly further increases T cells , macrophages , neutrophils , B cells and IL-10 hepatic levels but decreases CCL2 mRNA Significantly different from ND, *p < 0.05; **p < 0.01; ***p < 0.001; significantly different from WD, #p < 0.05; ##p < 0.01; ###p < 0.001.",yes
PMC4607445,Figure_2,oa_package/9b/43/PMC4607445.tar.gz,"['g002Cytokine production after intranasal infection with mucoid Sp isolate CHB756.', 'BAL fluids and lung homogenates were collected after 24 hours to measure cytokine production (), and CFUs were quantified from all mice at this lower infectious dose (A).', '0026) (B), which was also true for CFTR / mice infected with CHB1126 (data not shown).', 'However, in the case of the lung homogenates, although there were more median CFUs in the CFTR / than WT homogenates, the difference was not statistically significant for infections with CHB756 (A) or for CHB1126 (data not shown).', 'There were no significant differences in production of TNF- or CXCL1/KC in the lung homogenates (C and 2E) or the BAL (D and 2F) between CFTR / and WT-mice after 24 hours of infection.']",10.1371/journal.pone.0140335.g002,yes
PMC9798510,Figure_2,oa_package/f8/a3/PMC9798510.tar.gz,"['The resulting probability maps can then be converted to gray scale or color images and visualized as shown in s 2, A C and 3.', ' 2A D: Pathologist-marked regions versus classifier-generated spatial probability maps for each osteosarcoma subtype over whole slide images of tumor samples from dogs.', 'As a qualitative validation, s 2 and 3 depict pathologist-marked region boundaries within four dog and two human osteosarcoma surgical specimens covering each histologic subtype along with classifier-derived probability maps (one per histologic subtype) over the whole slide image.', 'For example, in 2D, there are several regions that were predicted to contain osteoblastic tumor cells.', 'pdf""/>\nSupplemental S3Example of a case where the trained histologic subtype classifier detects a region of osteoblastic (OB) tumor not annotated by the pathologist.']","Figure2 Pathologist-marked regions versus classifier-generated spatial probability maps for each osteosarcoma subtype over whole slide images of tumor samples from dogs. The probability maps (depicted below each whole slide image) are generated by applying the trained patch-level subtype classifier in a sliding window manner over the whole slide image using a window size of 256256 pixels. For more details, see . Original magnification, 10 ( ). CB, chondroblastic; FB, fibroblastic; GC, giant cell rich; N, necrosis; OB, osteoblastic; VR, vessel rich.",yes
PMC11117903,Figure_3,oa_package/c7/3c/PMC11117903.tar.gz,"['A new cancer diagnosis appropriately classified as malignant by artificial intelligence (AI): This patient in her 40s with a history of left breast carcinoma diagnosed 1 year prior, status post-left mastectomy with chemotherapy and hormonal therapy, presented with a palpable abnormality in the superficial lower outer left breast.']","Figure 3 A new cancer diagnosis appropriately classified as malignant by artificial intelligence (AI): This patient in her 40s with a history of left breast carcinoma diagnosed 1 year prior, status post-left mastectomy with chemotherapy and hormonal therapy, presented with a palpable abnormality in the superficial lower outer left breast. No new or suspicious findings were seen on the patients diagnostic mammogram. Correlating with the patients concern about a palpable lump, diagnostic ultrasound revealed an irregularly shaped, hypoechoic mass with angular margins that are non-parallel ( ), and Doppler shows no vascularity ( ). The AI program Koios recognized this mass as Probably Malignant ( ). This was returned as biopsy-proven invasive ductal carcinoma. Images obtained from the Icahn School of Medicine at Mount Sinai.",yes
PMC9756637,Figure_1,oa_package/52/16/PMC9756637.tar.gz,"['1; Table 1).', 'Cross-sectional Doppler ultrasound view of right temporal artery.', 'Arrow is pointing to hypoechogenic halo signTable 1Laboratory investigations in the patient during evaluationInvestigationResultReference rangeWhite blood cells ( 109/L)13.']",Fig. 1 Cross-sectional Doppler ultrasound view of right temporal artery. Arrow is pointing to hypoechogenic halo sign,yes
PMC10636816,Figure_3,oa_package/2e/78/PMC10636816.tar.gz,"[' 3A); however, high-glucose diets altered mitochondrial morphology within the CEP neurons (', ' 3B D).', ' 3A, B).', ' 3C).', ' 3D).', 'High-glucose diet induces mild neuronal mitochondrial elongation.', '0001High-sugar diets do not rescue ATP depletion caused by electron transport chain inhibitionIt has been theorized that ATP depletion may incite a negative feedback loop resulting in and enhancing neurodegeneration [50, 51].']","Fig. 3 High-glucose diet induces mild neuronal mitochondrial elongation. The number of mitochondria per dendrite. Average length of mitochondria per dendrite. Length of the longest mitochondria per dendrite. Sum of the lengths of all mitochondria within a dendrite in worms reared on control ( =249), 100mM glucose ( =127), or 100mM fructose ( =134) supplemented NGM plates. Three biological replicates were performed. Shapiro-Wilks normality tests determined all data sets were non-normally distributed. The KruskalWallis test followed by Dunns multiple comparisons test was used to establish -values. * <0.0332, ** <0.0021, *** <0.0002, **** <0.0001",yes
PMC8842755,Figure_3,oa_package/9a/36/PMC8842755.tar.gz,['Muscle histopathology in autopsy tissue from patients with liver disease.'],"Fig. 3 Muscle histopathology in autopsy tissue from patients with liver disease. Skeletal muscle tissue taken at autopsy from patients in the Congenital Muscle Disease Tissue Repository who showed variable degrees of liver pathology. H&E staining (A-C) reveals similar pathology that is characteristic of XLMTM, including myofiber smallness and increased numbers of fibers with internal nucleation, in the absence of inflammation or active myofiber degeneration. NADH staining (D-F) shows a pattern of organelle mislocalization that is characteristic of XLMTM in humans, with central aggregation of mitochondria and sarcotubular elements surrounded by an area of absent staining in the subsarcolemmal region. The muscle pathology findings in these three cases are extremely similar, despite differential levels of liver disease in these patients. One patient (panels A, D) had no histological evidence of liver disease, one patient (panels B, E) showed intrahepatic cholestasis at autopsy, and one patient (panels C, F) showed hepatic hemorrhage due to presumed hepatic peliosis.",yes
PMC10723712,Figure_1,oa_package/1a/5c/PMC10723712.tar.gz,"['To fill this gap in knowledge, we used an integrative multi-omics approach that combined CyTOF and 10X Chromium single-cell transcriptomics (A) to understand the complexity of the immune interactions taking place in the meningeal space during chronic T.', 'g001CyTOF confirms the expansion of innate and adaptive immune cells in the murine meninges during chronic T.', 'Overall, we detected a significant increase in the number of CD45+ cells (E and S1 Data) and an increased in number of the various immune subsets in the murine meninges at 30 dpi compared to na ve controls (F and S2 Data), without noticeable changes in cell frequency (G and S1 DataSupporting data for E Quantification of CD45+ cells in the meninges by CyTOF.', 's017"" position=""float"" content-type=""local-data"">S2 DataSupporting data for F Number of immune cell subsets as quantified by CyTOF.', 's018"" position=""float"" content-type=""local-data"">S3 DataSupporting data for G Frequency of various immune cell subsets in the meninges quantified by CyTOF.']",10.1371/journal.pbio.3002389.g001,yes
PMC9788411,Figure_1,oa_package/0e/62/PMC9788411.tar.gz,"['These structural and molecular changes in the OA cartilage are depicted in .', '166503531522568Healthy and osteoarthritic cartilage structure.', ' adapted from in reference [124].']","Figure 1 Healthy and osteoarthritic cartilage structure. In healthy cartilage, the fibrillar collagen network enriched in proteoglycans is a highly dense and negatively charged matrix. Osteoarthritis is a joint disorder involving the degeneration of articular cartilage, characterized by degradation of aggrecan proteoglycans by ADAMTS-4, loss and replacement of collagen type II by collagen type I, and increased apoptosis of chondrocytes; all these changes ultimately lead to the disturbance of cartilage homeostasis and loss of joint function. During OA, chondrocytes are exposed to pro-inflammatory cytokines mainly released by the synovial lining, such as TNF-, IL-6, IL-8, and IL-17, as well as nitric oxide (NO), which increases oxidative stress in the joint. In the synovial fluid, other changes also occur, such as loss of lubricin and increased amounts of fragmented aggrecan because of the catabolism in the articular cartilage.",yes
PMC10734789,Figure_1,oa_package/85/45/PMC10734789.tar.gz,['.'],"Figure 1. , Fluid-attenuated inversion recovery (FLAIR) MRI scans of an older patient with minimal white matter hyperintensities. , FLAIR MRI scans from equivalent areas of another older patient with extensive white matter hyperintensities and a cavitated lacune (arrow). , Susceptibility-weighted images from 2 different patients with SVD, showing multiple microbleeds (eg, marked with an arrow).",yes
PMC11290219,Figure_4,oa_package/e4/8e/PMC11290219.tar.gz,"[' 4).', '\nBoxplots depicting no significant differences in Ktrans or sPDGFR depending on A 42/40-, ATN- or APOE4-status.', 'A-F failed to reach statistical significanceAbbrevations: sPDGFR = soluble platelet-derived growth factor receptor ; A = amyloid-beta; APOE = apolipoprotein E gene; ATN: amyloid-beta positivity based on CSF-A 42/40, tau positivity based on CSF-p(181)tau, positivity for neurodegeneration based on CSF total tau\n\nCorrelation analyses between sPDGFR and ATN biomarkers.']","Fig. 4 Boxplots depicting no significant differences in Ktrans or sPDGFR depending on A42/40-, ATN- or APOE4-status. Significance testing using t-tests. Positivity of standard AD biomarkers was based on local accepted cut-off values. failed to reach statistical significance Abbrevations: sPDGFR=soluble platelet-derived growth factor receptor ; A=amyloid-beta; APOE=apolipoprotein E gene; ATN: amyloid-beta positivity based on CSF-A42/40, tau positivity based on CSF-p(181)tau, positivity for neurodegeneration based on CSF total tau",yes
PMC7456995,Figure_1,oa_package/2f/d9/PMC7456995.tar.gz,"['Fetal inflammatory responses were classified as stage 1 (chorionic vasculitis/umbilical phlebitis: neutrophils in the wall of any chorionic plate vessel or the umbilical vein), stage 2 (umbilical vasculitis: neutrophils in one or both umbilical arteries and vein), or stage 3 (necrotizing funisitis or concentric umbilical perivasculitis: neutrophils, cellular debris, eosinophilic precipitate, and/or mineralization arranged in a concentric band, ring, or halo around one or more umbilical vessels) ().', 'Placental sections showing the three stages of maternal and fetal inflammatory response.']",Figure 1 Placental sections showing the three stages of maternal and fetal inflammatory response. Maternal inflammatory responsestage 1. Maternal inflammatory responsestage 2. Maternal inflammatory responsestage 3. Fetal inflammatory responsestage 1. Fetal inflammatory responsestage 2. Fetal inflammatory responsestage 3.,yes
PMC5770272,Figure_2,oa_package/84/3a/PMC5770272.tar.gz,[],Fig.2 Esophagogram ( ) showing leakage of water soluble contrast material (arrows) into the right side of the chest and/or mediastinum ( ).,yes
PMC5706804,Figure_1,oa_package/a3/6c/PMC5706804.tar.gz,"['Examples of control probe expression in FFPE tissues from a prospectively collected cohort of samples(A and D) POLR2A, (B and E) PPIB, (C and F) UBC.']","Figure 1 Examples of control probe expression in FFPE tissues from a prospectively collected cohort of samples ( and ) POLR2A, ( and ) PPIB, ( and ) UBC. A, B and C are examples from colorectal tissue, D, E and F are examples from ovarian tissue. Note the increasing expression level from POLR2A to UBC.",yes
PMC6701449,Figure_2,oa_package/c3/ab/PMC6701449.tar.gz,"['The most experienced author (ALHH) first graded all EOS hand radiographs using the TOCI staging system ().', 'Table 1Conversion table from Tanner Whitehouse III (TW3) descriptors to Thumb Ossification Composite Index (TOCI)Adductor sesamoid (AS)Thumb PP epiphysisThumb DP physisAS-AS+PPEPPFPPG1PPG2PPHPPIDPNon-IDPIModified TW3 descriptorsTOCI stageAbsent (A)Ossified (O)Uncovered (E)Covered (F)Early capped (G1)Advanced capped (G2)Partial fusion (H)Fused (I)Open (non-I)Fused (I)1101000001021001000010301010000104010010001050100010010601000100017010000100180100000101Adductor sesamoid: A: absence of adductor sesamoid bone, designated as AS-; O: ossified of adductor sesamoid bone, designated as AS+Thumb PP epiphysis: E: TW3 stage E (uncovered epiphysis), designated as PPE; F: TW3 stage F (covered epiphysis), designated as PPF; G1: TW3 stage G1(early capped epiphysis), designated as PPG1; G2: TW3 stage G2(advanced capped epiphysis), designated as PPG2; H: TW3 stage H (partial fusion epiphysis), designated as PPH; I: TW3 stage I (fused epiphysis), designated as PPIThumb PP physis: Non-I: all TW3 stage before I (non-fused epiphysis), designated as PPNon-I; I: TW3 stage I (fused epiphysis), designated as DPIPP, Proximal Phalangeal; DP, Distal Phalangeal; 1, present; 0, absentEOS version for Thumb Ossification Composite Index (TOCI): a) width of thumb proximal phalangeal epiphysis same as that of the metaphysis; b) width of thumb proximal phalangeal epiphysis exceeds that of the metaphysis (covered epiphysis); c) roundish covered (without capping) ulnar corner of thumb proximal phalangeal epiphysis; d) appearance of the ossified adductor sesamoid bone; e) early capping of ulnar corner of thumb proximal phalangeal epiphysis; f) advanced capping of ulnar corner of thumb proximal phalangeal epiphysis; g) thumb distal phalangeal epiphysis completely fused; h) thumb proximal phalangeal epiphysis partially fused (both black and white band); i) thumb proximal phalangeal epiphysis completely fused.']",Fig. 2 EOS version for Thumb Ossification Composite Index (TOCI): width of thumb proximal phalangeal epiphysis same as that of the metaphysis; width of thumb proximal phalangeal epiphysis exceeds that of the metaphysis (covered epiphysis); roundish covered (without capping) ulnar corner of thumb proximal phalangeal epiphysis; appearance of the ossified adductor sesamoid bone; early capping of ulnar corner of thumb proximal phalangeal epiphysis; advanced capping of ulnar corner of thumb proximal phalangeal epiphysis; thumb distal phalangeal epiphysis completely fused; thumb proximal phalangeal epiphysis partially fused (both black and white band); ) thumb proximal phalangeal epiphysis completely fused.,yes
PMC8195544,Figure_2,oa_package/7a/6f/PMC8195544.tar.gz,['POCUS of the heart parasternal long-axis view note the RV is not collapsed.'],Figure 2 POCUS of the heart parasternal long-axis view note the RV is not collapsed. There is a small PCE. A PLEis noted as it is posterior to the DAo. POCUS:point-of-care ultrasound; PCE:pericardial effusion; PLE:large pleural effusion; DAo:descending aorta; RV:right ventricle,yes
PMC6154927,Figure_2,oa_package/c4/29/PMC6154927.tar.gz,"['2a), aged male hAPP-SL (Thy1-hA PPLond/Swe) mice were used.', 'Brain atrophy and cholinergic degeneration is more pronounced in aged versus young adult C57BL/6 mice following stroke.', '0001All mice were housed under a 12-hour light/dark schedule with ad libitum access to food and water.', '2a and c).', '2a and c).', '2b and c).', '2d and e).']","Fig. 2 Brain atrophy and cholinergic degeneration is more pronounced in aged versus young adult C57BL/6 mice following stroke. Representative 4 images of Nissl-stained whole brain sections from 3 and 18 month-old (mo), sham- and stroke-operated C57BL/6 mice at 8 weeks post-surgery. Representative 2 images of Nissl-stained cortical layers I-VI of the ipsilateral primary somatosensory cortex in 3 and 18 mo, sham- and stroke-operated mice at 8 weeks post-surgery. Scale bar, 250 m. Quantification of the lateral ventricle (left graph) in the ipsilateral hemisphere revealed significant ventricle enlargement in the 18 mo stroked mice relative to the age-matched sham-operated mice. Furthermore, the 18 mo stroked mice had a significantly larger ventricle area compared to the 3 mo stroked mice. Quantification of cortical thickness (right graph) in the ipsilateral hemisphere revealed significant tissue loss or shrinkage in both the 3 and 18 mo stroked mice relative to age-matched sham-operated mice. Furthermore, the 18 mo stroked mice had significantly more tissue loss compared to the 3 mo stroke mice. Representative 5 images of choline acetyltransferase (ChAT)-immunolabeled neuronal cell bodies, their neurites, and innervating projection fibers in the medial septum of the basal forebrain in 3 and 18 mo, sham- and stroke-operated C57BL/6 mice at 8 weeks post-surgery. Many neurites (arrows) in the stroked mice displayed qualitative degenerative changes, including decreased length relative to sham mice. Scale bar, 100 m. Quantification of cholinergic somas, neurites, and fibers revealed a significant reduction of ChAT+ staining in both the 3 and 18 mo stroked mice relative to the age-matched sham mice. Furthermore, the 18 mo stroked mice exhibited significantly more loss of ChAT+ staining compared to the 3 mo stroked mice. Data represent mean SEM. * <0.05, ** <0.01, and **** <0.0001",yes
PMC6233134,Figure_1,oa_package/05/f4/PMC6233134.tar.gz,['Examples of the model s localizations are shown in .'],"Fig. 1. , shows a typical radiograph, which is provided as an input to the model. , depicts a heat map overlaid on the radiograph. When the model determines that a fracture is present, the heat map represents the models confidence that a particular location is part of the fracture, with yellow and blue being more and less confident, respectively. ( ) Close-up views of four additional example inputs and heat map overlays.",yes
PMC2930857,Figure_1,oa_package/2a/7c/PMC2930857.tar.gz,"['Foci of myocytolytic necrosis and degeneration are observed microscopically with an intense mononuclear inflammatory infiltrate and intense parasitism of myofibers (A).', 'In CCC, micropathology reveals focal and diffuse chronic fibrosing myocarditis (C and 1D).', 'Diffuse foci of myocardial micronecrosis are present and associated with an inflammatory infiltrate composed predominantly of lymphomononuclear cells and interstitial fibrosis, one of the most prominent features (B) The NLR of 136 enrolled patients ranged from 1.', '005) (B).']","Figure 1. Neutrophillymphocyte ratio (NLR) in different groups. A, ROC curve analysis of NLR; B, differences in peripheral blood NLR of tumor patients with different degrees of differentiation with test.",yes
PMC11487719,Figure_2,oa_package/aa/3e/PMC11487719.tar.gz,"[' 2)\nRadiographic examination of dogs in various groups.', '(d-f) Normal abdominal radiograph of all ZnO NPs receiving groups\nThe abdominal ultrasonographic examination of dogs in the control group showed normal homogenous parenchymal echogenicity of the liver with smooth capsule and regular contour.']","Fig. 2 Radiographic examination of dogs in various groups. ( - ) Left lateral thoracic radiograph representing, ( ) control group has normal lung and cardiac silhouette. (b & ) groups receiving 50 and 100mg ZnO NPs, respectively, showed mild to moderate radiographic changes. Note: pleural effusion (white arrow), vascular pattern of pneumonia (yellow arrow), bronchial pattern of pneumonia (blue arrow). ( - ) Normal abdominal radiograph of all ZnO NPs receiving groups",yes
PMC2956888,Figure_3,oa_package/82/cf/PMC2956888.tar.gz,"[' 3).', '', 'Magnification 28,000Biochemical analysis of residual lipids in FFPE samples from the pons (case 3, day 119) which were rich in ballooned granulovacuolar neurons, revealed a threefold to sixfold elevation of gangliosides (GM1, GM2, GM3 types), lactosylceramide and globotriaosylceramide as compared to the controls (data not shown).', ' 3) and axons in our cases of pSap deficiency resembled ultrastructural changes in the neuroaxonal dystrophy classified as a macroautophagy process [46] that also featured a high degree of ubiquitination [11, 42] combined with lysosomal enzyme activities [63].']","Fig.3 Ultrastructure of a subcortical neuron in case 1 (day27) of prosaposin deficiency. A dense population of lysosomes containing pleiomorphic condensed deposits. Some of the deposits resemble degenerated mitochondria ( ), for comparison refer to intact mitochondrion ( , ). Magnification 28,000",yes
PMC2988799,Figure_5,oa_package/5e/14/PMC2988799.tar.gz,['Subcortical brain activations.'],"Figure 5 . Row (A) depicts thalamus activity (in white circle) and row (B) depicts cerebellum activity from all 5 subjects. Left panels are sagittal sections, while the middle panels are coronal and the right panels axial sections.",yes
PMC6824645,Figure_5,oa_package/42/68/PMC6824645.tar.gz,['Microscopic image p 63 stain positive for mioepithelial cells and negative for epithelial cells.'],"Figure 5 Microscopic imagep 63 stain positive for mioepithelial cells and negative for epithelial cells. HE coloration, 10.",yes
PMC4152333,Figure_8,oa_package/6c/1b/PMC4152333.tar.gz,"['A shows representative Western blot analysis of eNOS and nNOS expression in wt and mdx gastrocnemius muscles with and without pGz treatment.', '05, B) without any detectable effect on the expression of nNOS (P 0.', '05, C).', 'g008nNOS and eNOS protein levels in muscles from pGz-treated mice.']",10.1371/journal.pone.0106590.g008,yes
PMC2765174,Figure_1,oa_package/3f/36/PMC2765174.tar.gz,"['[2] However, when mammography and USG fail to fully evaluate a finding, we have found breast MRI to be a useful complementary study to conventional breast imaging modalities [].', ' (A,B)Mammogram, craniocaudal projection (A) and maximum-intensity projection (MIP) DCE-MRI (B).', '1 shows a case of mammographically occult breast cancer identified by DCE-MRI in a known breast cancer (BRCA1) gene mutation carrier.', ""Table 3High-risk patientsBRCA1 or BRCA2 mutation, or untested first-degree relative of known carrierChest radiation between age 10 30 years for Hodgkin's lymphomaLifetime risk of 20 25% as determine by statistical risk assessment models such as BRCAPRO or GailOther genetic mutations, including p53 and Cowden1 (A,B)Mediolateral oblique projection of the left breast (A) in a BRCA1 gene mutation carrier."", '2 (A,B)T1W non-fat-saturated axial image (A) demonstrates a scar in the right breast (arrow) in a patient with prior lumpectomy and questionable increasing architectural distortion on mammogram.']","Figure 1 (A,B) Mammogram, craniocaudal projection (A) and maximum-intensity projection (MIP) DCE-MRI (B). This was a 48-year-old woman noted to have a density (arrow) in the craniocaudal projection only and architectural distortion (arrowheads) in the subareolar location in both standard mammographic views. The anterior lesion was biopsied and proved to be invasive mammary carcinoma with ductal and lobular features. The density could not be identified on multiple additional diagnostic views. DCE-MRI was recommended to further evaluate the initial mammographic finding. The lesion (arrow) was identified by MRI and located at the 6 o'clock position, far posterior to the chest wall. This lesion was also biopsied and proven to be a second area of invasive mammary carcinoma",yes
PMC11707747,Figure_3,oa_package/3d/b3/PMC11707747.tar.gz,"['ResultsH EQualitative analysis\nA shows a WSI example of the H E real and virtual stains.', 'B shows a magnification series of concentric regions from the WSI.', '\nA, Example of real (left) and virtual (right) stains for a H E WSI.', 'C shows examples of the segmentations.']","Figure 3 Example of real (left) and virtual (right) stains for a H&E WSI. Magnification series of real (left) and virtual (right) stains for concentric regions from the WSI at 10 (top), 20 (middle), and 40 (bottom) magnifications. Example of the segmentations on real (left) and virtual (right) stains obtained from automatic histologic subtyping.",yes
PMC2699342,Figure_1,oa_package/a6/d0/PMC2699342.tar.gz,"['A, B: Histological staining (Hematoxylin-Eosin) of the retina of sham-operated (A) and 2VO ligated 8 days after surgery (B).']","Figure 1 . C, D: Histological staining (Masson Trichromic staining) of the optic nerve of sham (C) and 2VO animals 8 days after surgery (D). E-R: Optic nerve staining of sham- (E-L) and 2VO- operated animals 8 days after surgery (M-R) with the markers listed in the figure. Images are obtained by conventional fluorescence microscopy. Bars: 100 m (A, B); 200 m (C-R). : outer nuclear layer; : outer plexiform layer; : inner nuclear layer; : inner plexiform layer; : ganglion cell layer.",yes
PMC5063346,Figure_3,oa_package/4d/7b/PMC5063346.tar.gz,"['The dose-response curves showed half-maximal responses of 10 M for MFX and SUL and 100 M for CsA (A).', 'g003Activity of the candidate drugs in yeast.', '0001) (B), whereas CsA decreased ER stress by 32% (p = 0.', '002) (C).']",10.1371/journal.pone.0164465.g003,yes
PMC4345727,Figure_1,oa_package/9a/de/PMC4345727.tar.gz,"['Meanwhile, T2-weighted sagittal images revealed a heterogeneous intensity change, accompanying a central area of hyperintense signals with a hypointense peripheral border at the L4 vertebra ().']",Fig. 1. MRI of the lumbar spine. Sagittal T1-weighted image reveals the extradural space-occupying lesion with a hyperintense signal relative to that of the spinal cord under the L4 vertebra. Sagittal T2-weighted image reveals a heterogeneous intensity change accompanying the central area of hyperintense signals with a hypointense peripheral border. Sagittal T1-weighted image after gadolinium administration shows no enhancement.,yes
PMC8461564,Figure_5,oa_package/6f/66/PMC8461564.tar.gz,['Cellular biomarkers of ACR in IT grafts.'],"Fig. 5 Cellular biomarkers of ACR in IT grafts. H&E-stained NHP allograft specimens of the small intestine on days 0, 2, and 6 after transplantation. Insets: FOV with corresponding location marked by a dashed line. .",yes
PMC7805200,Figure_3,oa_package/94/9d/PMC7805200.tar.gz,"[' 3a).', ' 3b, c).', ' 3d h).', 'Such lumen was occluded by fibrous tissue (arrowhead)Discussion and conclusionsThe etiology of GCP was probably chronic inflammation and ischemia occurring at the anastomotic site postoperatively.']","Fig. 3 Histopathological view of the resected specimen near the site of endoclip attachment. HE-stained weakly magnified image revealed thickening of the mucosa and hyperplasia of the crypt epithelium. Magnified image of the blue square showed pseudopyloric gland proliferation with cystic dilation in the mucosa. The muscularis mucosa was entangled and elevated radially. Magnified image of the red square showed mucosal damage such as fibrin exudation along with necrosis. No atypical cells were found in the resected specimen. Histopathological views of the serial resected specimens including the section shown in ( ). Elastica van Gieson-stained weakly magnified images showed muscular vessels that developed between muscularis and submucosa. , Magnified images of the yellow and green squares showed muscular vessels with an irregularly thickened lumen (arrows). Such lumen was occluded by fibrous tissue (arrowhead)",yes
PMC10550728,Figure_11,oa_package/be/1c/PMC10550728.tar.gz,[],Supplemental Fig.5,yes
PMC10680640,Figure_6,oa_package/8e/d9/PMC10680640.tar.gz,"['4-month-old Tmem106b / PS19 mice compared to PS19 mice (Supplementary ), indicating that elevated glia activation seen in 8.', 'We found a dramatic increase of lipofuscin signals in the hippocampal CA3 region of Tmem106b / PS19 mice, compared to a subtle accumulation of lipofuscin signal in the PS19 mice and no changes in lipofuscin signal in Tmem106b / mice (A and 6B), indicating that TMEM106B deficiency combined with Tauopathy causes severe lysosomal defects.', '5-month-old Tmem106b / PS19 mice compared to PS19 mice (C and 6D).', '4-month-old Tmem106b / PS19 mice, while PS19, Tmem106b / and WT mice do not show any obvious abnormalities at this age(E and 6F), indicating that loss of TMEM106B in PS19 mice likely results in exacerbated lysosomal trafficking defects and/or other abnormalities in the AIS.', 'Moreover, we observed an accumulation of large CathD-positive clusters out of NeuN-positive neurons in the hippocampus of Tmem106b / PS19 mice, indicating upregulation of CathD in glial cells (C).', 'In this study, we found that the levels of lysosomal enzyme CathD were reduced in CA3 neurons (C and 6D), but significantly increased in microglia in 8.', '4-month-old Tmem106b / PS19 mice (E and 6F).', '566707v1-f0005"" position=""float""/>.']","Figure 6. TMEM106B deficiency results in increased lipofuscin accumulation and lysosomal alterations in PS19 mice Representative image of lipofuscin autofluorescence in the hippocampal CA3 region of 8.5-month-old WT, , PS19, and PS19 mice. The autofluorescence intensity was quantified in B. n=46. Data presented as mean SEM. One-way ANOVA tests with Bonferronis multiple comparisons: **, p<0.01; ****, p<0.0001. Scale bar = 100 m. Representative image of NeuN, CathD and hTau co-immunostaining in the hippocampus of 8.5-month-old WT, , PS19, and PS19 mice. Relative CathD intensity in NeuN-positive neurons was quantified in D. n=3. Number of neurons =121125. Data presented as mean SEM. Unpaired Students t-test: ***, p<0.001. Scale bar: 10 m. Representative image of CathD and AnkG co-immunostaining in cerebellum sections from 5 to 5.4-month-old WT, , PS19 and PS19 mice. The percentage of axon initial segments with CathD-positive vesicles was quantified in F. n=3. Data presented as mean SEM. Unpaired Students t-test: *, p<0.05. Scale bar: 1 m. For Zoom-in images, Scale bar: 0.5 m.",yes
PMC10075123,Figure_1,oa_package/c7/d6/PMC10075123.tar.gz,"['AD as an autoimmune disorder: the responsibility of oral dysbiosis: In addition to genetic and environmental factors, an important correlation between oral health and AD has been described, with a link between infections resulting from oral dysbiosis ().', 'Oral dysbiosis may lead to Alzheimer s disease pathology.', 'All these alterations destabilize the peripheral immune-inflammatory balance and exacerbate neuroinflammation and neurodegeneration leading to AD pathology ().']","Figure 1 Oral dysbiosis may lead to Alzheimers disease pathology. An imbalance between oral pathogens and AMP levels may lead to oral dysbiosis, resulting in exacerbated proinflammatory responses. These peripheral inflammatory mediators lead to detrimental effects on submandibular AQP5 levels and salivary gland function, and BBB integrity contributing to neurodegeneration in Alzheimers disease pathology. AMP: Antimicrobial peptide; AQP: aquaporin; BBB: brain-blood barrier; TNF: tumor necrosis factor.",yes
PMC7348366,Figure_2,oa_package/4b/34/PMC7348366.tar.gz,"['001 2 2 NCCN-IPI IPI 5 OS 74% 54% 2 5.', '021 2NCCN-IPI A B IPI C D T PTCL 4 Ann Arbor ECOG 2 2 LDH PFS OS ALK+ ALCL PFS OS P 0.']",2 NCCN-IPIABIPICDTPTCL,yes
PMC2879083,Figure_3,oa_package/ad/b3/PMC2879083.tar.gz,"['Matrix metalloprotease-2/9 activity is induced around the occlusion sitea, In situ zymography 5 hours post-embolization indicates increased gelatinolytic activity (green) around fibrin clot (red).']","Figure 3 Matrix metalloprotease-2/9 activity is induced around the occlusion site , In situ zymography 5 hours post-embolization indicates increased gelatinolytic activity (green) around fibrin clot (red). Scale bar, 10m. , Quantification of gelatinolytic activity around clot and microsphere occluded microvessels (mean s.e.m.; n=35 mice and 110 clot-occluded vessels and n=10 mice and 75 microsphere-occluded vessels. *p<0.05, one-way ANOVA). , Quantification of the percentage of extravasated clots and microspheres in mice treated with MMP 2/9 inhibitor SB-3CT. (mean s.e.m.; n=4 mice per treatment group). (***p<0.001 two-tail Students t-test).",yes
PMC4564603,Figure_2,oa_package/59/71/PMC4564603.tar.gz,"['These bubbles stained on formalin fixed, frozen lung with fat stains ().', '001""/>(a) The lower portion demonstrates the necrotic bone marrow compared to the upper viable marrow (2x original magnification, H E stain).']","Figure 2 (a) The lower portion demonstrates the necrotic bone marrow compared to the upper viable marrow (2x original magnification, H&E stain). (b) The border of the viable and necrotic marrow shows sickling of red cells (40x original magnification, H&E stain).",yes
PMC8571281,Figure_2,oa_package/39/2d/PMC8571281.tar.gz,"['During external-internal percutaneous transhepatic biliary drainage,[4] at times, the tube appears to be outside the confines of the second and third parts of the C loop of the duodenum [].', ':A 49-year-old patient who underwent percutaneous transhepatic biliary drainage internalization.']",Figure 2: A 49-year-old patient who underwent percutaneous transhepatic biliary drainage internalization. Amplatz guidewire (arrow) appears outside the second and third parts of the duodenum after crossing the ampulla.,yes
PMC7498657,Figure_2,oa_package/38/87/PMC7498657.tar.gz,"['7 cm of the gastrocnemius tendon appeared thinner compared to areas more proximal ().', 'T1-weighted pre- and post-contrast sagittal MR images of a German shorthaired pointer dog with rupture of the common tendons of the biceps femoris, gracilis, and semitendinosus (accessory ischial muscles) in the left pelvic limb.']","Figure 2 T1-weighted pre- and post-contrast sagittal MR images of a German shorthaired pointer dog with rupture of the common tendons of the biceps femoris, gracilis, and semitendinosus (accessory ischial muscles) in the left pelvic limb. A vitamin-E capsule (asterisk) is positioned at the level of the left calcanean tendon visible in images . T1-weighted pre-contrast sagittal image of the common tendons of the accessory ischial muscles (long arrow), gastrocnemius tendon (arrowhead), and superficial digital flexor tendon (short arrow). The common tendons of the accessory ischial muscles are thickened just proximal to the calcaneus. T1-weighted post-contrast sagittal image showing disruption and contrast enhancement of the common tendons of the accessory ischial muscles (long arrow), contrast enhancement surrounding the gastrocnemius tendon with heterogenous signal intensity (arrowhead), and contrast enhancement surrounding the superficial digital flexor tendon (short arrow).",yes
PMC9763677,Figure_1,oa_package/a9/03/PMC9763677.tar.gz,"['Pulmonologists observed an endobronchial mass in the right main bronchus, reaching 1 cm from the main carina and completely blocked the right main bronchus (A).', 'The patient underwent endobronchial debulking to restore the patency of the right bronchial tree (B).', 'Images of the endobronchial assessments.', 'An AERO stent (Merit Medical Systems, Utah, USA) was implanted (diameter, 10 mm; length, 40 mm) to re-establish the main right bronchus airway permeability after debulking (C).', 'On the same day, the patient underwent bronchoscopy to confirm tumour progression occluding the stent (D) and stenosis of the distal trachea up to 50 %.']","Fig. 1 Images of the endobronchial assessments. A. Endobronchial main right bronchus complete obstruction by an endoluminal mass. B. Rigid bronchoscopy view of the right main bronchus during mechanical debulking. C. Main right bronchus permeability restored after Aero stent placement. D. Tumour endobronchial progression occluding the stent and englobing the carina (red arrow). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)",yes
PMC3271068,Figure_4,oa_package/f0/c2/PMC3271068.tar.gz,"['The course of PbAluc infection in control, -PD-L1- and -CTLA-4-treated mice (\nA C\n) was similar to the course of PbA infection (A C), confirming that insertion of the luciferase gene had not significantly altered the basic biology of the parasite, although the onset of ECM was slightly delayed ( -CTLA-4-treated mice developed ECM on day 10 post infection; -PD-L1-teated mice developed ECM on day 11 and control mice were euthanised on day 18).', 'Nevertheless, after day 7 of infection, whole body (\nD\n), head (\nE\n) and isolated brain (\nF,G\n) parasite burdens were significantly higher in -CTLA-4- and -PD-L1-treated mice than in control mice.', 'It should be noted that the detection of a weak luminescence signal in the brains of control (no ECM) mice in F simply reflects the presence of luminescent parasites in the circulating blood in all organs.', 'g004Onset of ECM after CTLA-4 and PD-1 blockade in PbA-infected BALB/c mice is associated with parasite accumulation in the brain.']",10.1371/journal.ppat.1002504.g004,yes
PMC3726689,Figure_5,oa_package/c7/01/PMC3726689.tar.gz,"['g005Age-dependent expression of Ig-Hepta mRNA and accumulation of surfactants and Mmp12.', 'g007""/>Ontogeny of Ig-Hepta mRNA Expression and Accumulation of SurfactantsTo know the timing of Ig-Hepta expression in the lung, the expression of Ig-Hepta mRNA was quantified in fetal, neonatal, young, and adult lungs of wild-type mice by real-time PCR (A).', '\nB shows SatPC contents in the lungs of Ig-Hepta\n+/+ and Ig-Hepta\n / mice before and after birth up until the age of 40 weeks.']",10.1371/journal.pone.0069451.g005,yes
PMC11444080,Figure_10,oa_package/37/9f/PMC11444080.tar.gz,[],Figure 9 ( ) Representative images of PLA for Fyn-tau (white dots) and NR2B-PSD95 (cyan dots) co-labelled with a dendritic marker MAP2 (green/magenta) comparing SAR versus VEH groups ( ) demonstrating reduced Fyn-tau and NR2B-PSD95 interactions in SAR group. Scale bar 100m. Bar graphs displayed all data points and expressed as mean SEM. Dots represent individual animals. Two-group comparison used the Students -test or the MannWhitney test.,yes
PMC6849156,Figure_9,oa_package/2b/28/PMC6849156.tar.gz,"['The laparoscopic team augmented defect closure by placing a 15 15 cm bilaminar mesh over the defect, fixing it to the diaphragm with interrupted nonabsorbable sutures ().', '15 15 bilaminar mesh placement.', 'The interval between arrival at hospital and surgery was 90 min.']",Fig. 9 1515 bilaminar mesh placement.,yes
PMC10460313,Figure_2,oa_package/76/98/PMC10460313.tar.gz,"[' 2; Video 12).', '15-year-old girl with juvenile scoliosis.', 'b Corresponding conventional, stitched X-ray of the spineWritten informed consent for publication of this report was obtained from the patient.']","Figure 2. Histopathological examination of lung tissue. (a) Actinomycetes colony with surrounding small abscesses (hematoxylineosin, 400) and (b) evidence of organizing pneumonia in the lung tissue (hematoxylineosin, 100).",yes
PMC8369003,Figure_7,oa_package/0f/aa/PMC8369003.tar.gz,"['Like aged 5XFAD/Bad / mice, 9-month-old APP/PS1/Bad / mice showed reduced number and burden of A plaques in both cortex and hippocampus compared with their age-matched APP/PS1 mice (s 7A and 7B).', 'Confocal microscopy revealed significant morphological changes of plaque-associated microglia in the cortex of APP/PS1 mice compared with WT mice, and importantly the morphological changes of these microglia were partially reverted in APP/PS1/Bad / mice (s 7C and 7D), consistent with the results observed in 5XFAD mice.', 'Furthermore, in line with the findings in 5XFAD mice, CD68 expression in plaque-associated microglia was increased (s 7E and 7F) but the percentage of ASC speck-containing microglia was decreased in the cortex of APP/PS1/Bad / mice (36.', '43%) (s 7G and 7H).', 'APP/PS1 mice exhibited significant poor performance in Y-maze test compared with WT mice, while this memory deficit was rescued in APP/PS1/Bad / mice ( 7I).', ' 7Bad loss inhibits A deposition, neuroinflammation and memory deficits in APP/PS1 mice(A) A plaques in half-brain slices of 9-month-old WT, APP/PS1 and APP/PS1/Bad / mice were detected by immunofluorescent staining with anti-A antibody (6E10).']","Figure6 BAD is involved in activation of microglial NLRP3 inflammasome via ROS-oxidative mtDNA axis (A) Detection of BAD mitochondrial translocation in plaque-associated microglia in the cortex of 6-month-old mice (left panels). Scale bar, 5m. The co-localization of BAD and mitochondria was analyzed by the overlap of BAD and COX-IV immunofluorescent signals (right panels). (B) Immunoblotting analysis of caspase-1 (Casp-1) cleavage in 6-month-old mouse brain extracts. The ratio of Casp-1 p20 relative intensity to -actin was quantified (n= 3). Data were presented as mean SEM. p< 0.01, p< 0.001, one-way ANOVA test. (C) ASC specks were detected in plaque-associated microglia in the cortex of 6-month-old WT, 5XFAD and 5XFAD/ mice. Scale bar, 20m. The arrows indicate ASC speck-containing microglia. z stack images showed ASC speck was localized inside of microglia. The percentage of ASC speck-containing microglia was quantified (n= 3). Data were presented as mean SEM. p< 0.01, one-way ANOVA test. (D) Detection of BAD mitochondrial translocation in plaque-associated microglia in the frontal cortex of patients with Alzheimers disease and age-matched healthy control brains (left panels). Scale bar, 5m. The co-localization of BAD with mitochondria was analyzed by the overlap of BAD and COX-IV immunofluorescent signals (right panels). (E) ASC specks were detected in plaque-associated microglia in the frontal cortex of patients with Alzheimer's disease and control brains. Scale bar, 20m. The arrows indicate ASC speck-containing microglia. The percentage of ASC speck-containing microglia was quantified (n= 5). Data were presented as mean SEM. p< 0.001, students -test. (F) Oxidized mitochondrial DNA was detected by immunofluorescent analysis with anti-8-OHdG antibody in plaque-associated microglia in the cortex of 6-month-old WT, 5XFAD and 5XFAD/ mice. Scale bar, 20m. The average area of 8-OHdG in plaque-associated microglia was quantified (n= 5). Data were presented as mean SEM. p< 0.01, one-way ANOVA test. (G) Oxidized mitochondrial DNA was detected in plaque-associated microglia in the frontal cortex of patients with Alzheimers disease and age-matched healthy control brains. Scale bar, 20m. The average area of 8-OHdG in plaque-associated microglia was quantified (n= 5). Data were presented as mean SEM. p< 0.001, students -test. See also .",yes
PMC10579338,Figure_2,oa_package/83/1f/PMC10579338.tar.gz,"[' 2A,B).', ' 2).', '(A,B) Software-based detection of carcinoma cells: Qu-Path (version 0.', 'Quantifying tumour cell number on a reference surgical specimenA representative case of the tubular (intestinal) differentiated adenocarcinoma of the oesophagus treated with primary surgery was selected.', 'tif"">Supplementary .']","Figure 2 ( , ) Software-based detection of carcinoma cells: Qu-Path (version 0.3.2) marks the cells interpreted by the software as carcinoma cells. Surrounding stromal cells are not marked. A control by morphologically trained personnel/pathologists per case is therefore possible and reasonable.",yes
PMC3521654,Figure_5,oa_package/11/16/PMC3521654.tar.gz,"['Endogenous EBNA2, RBP-J , and actin control were expressed at similar levels, and both RBP-J and EBNA2 were efficiently immunoprecipitated by their corresponding antibodies from each cell lysate (A).', 'g005NPM1-mediated EBNA2 recruitment onto the LMP1 promoter is essential for the maintenance of lymphoblastoid cells.', 'Although EBNA2, RBP-J , and actin were expressed at similar levels in the control and in all shRNA-transduced BJAB-E2/LMP1-Luc cell lines, NPM1 deficiency caused a nearly complete dissociation of EBNA2 from the LMP1 promoter (D).', 'As expected, the EBNA2 target genes, such as c-MYC and LMP1, were expressed in a way that was inversely correlated with the NPM1 knockdown efficiency (E).', 'Strikingly, the cell proliferation of IB4 cells was halted when only 32% of the NPM1 expression was retained, whereas these cells died over time when an 89% knockdown efficiency of NPM1 was reached (F).', 'We found that the patterns of cell growth were not altered by NPM1 depletion in the context of BJAB cells (G).', 'IB4 cells treated with or without ATP depletion were subjected to IP and ChIP analyses exactly as described in A and 5D.', 's003""> S3\nThe EBNA2 binding domain of NPM1 associated with RBPJ, in relation to \n\n\n\n.']",10.1371/journal.ppat.1003084.g005,yes
PMC3085152,Figure_3,oa_package/95/4d/PMC3085152.tar.gz,"['Two cases of traumatic blood cysts and two cases of mucous glands retention cysts were located in the bulbar conjunctiva [].', 'A retention cyst occupying the temporal fornix (A).']","Figure 3 A retention cyst occupying the temporal fornix (A). Photomicrograph showed the cubical cell layer lining of the cyst with area of epithelium rich in goblet cells (H and E, 25) (B)",yes
PMC6548814,Figure_1,oa_package/b2/4a/PMC6548814.tar.gz,"['Mast Cell Accumulation in the Lung and the Mechanisms BehindMast Cell Accumulation in the Lung of Asthma PatientsSeveral studies suggest that the presence or accumulation of mast cells at certain compartments of the lung are pathological features of allergic asthma ().', 'Mast cells in mouse and human lung.']","Figure 1 Mast cells in mouse and human lung. Integrin-7 mast cell progenitors (MCps) are found in mouse and human peripheral blood ( , ). In mice with acute allergic airway inflammation, MCps are recruited to the lungs in a process dependent on 41 and 47 integrins on the MCp and on VCAM-1 expressed in the endothelium ( ). After the acute phase, three mast cell (MC) populations can be identified by flow cytometry, MCps expressing high levels of integrin 7, immature/induced MCs (iMCs) expressing intermediate levels of integrin 7, and mature MCs ( , ). The iMC gradually loses the expression of integrin 7 and mature, thereby expanding the resident lung MC population. In the mouse trachea and in the proximal bronchi of unprovoked mouse airways, MCs express the MC proteases mMCP-1 and-2, mMCP-4,-5-6, and-7 and CPA3, while the MCs induced by allergic airway inflammation located in the bronchovascular bundles of the lung and the epithelial lining of the large bronchi express mMCP-1,-2 and -6,-7 ( ). In the human lung MC and MC coexist, MC is more frequently found in the bronchi, bronchioles and alveolar parenchyma, whereas MC dominates in pulmonary vessels and pleura ( ) In the human bronchi, patients with Th2-high asthma have an increased number of intraepithelial MCs ( ). Genetic analyses suggest that the MCs in this location mainly express tryptase and CPA3 ( ). The number of MCs are increased in the airway smooth muscle of asthma patients ( , ). In diseased asthma patients, there are an increased number of mast cells in the distal airway, especially in the smooth muscle and mucous glands ( ). Uncontrolled atopic asthmatics have a high number of mast cells in the alveolar parenchyma ( ). The histology pictures shown are from hematoxylin/eosin-stained lung sections of house dust mite-sensitized wild-type BALB/c mice obtained from our unpublished experiments.",yes
PMC6870550,Figure_3,oa_package/c1/a0/PMC6870550.tar.gz,"['At month 12 of the placebo-controlled phase 3 studies, there had been a mean increase from baseline of approximately 2 mm in endometrial thickness as measured by MRI in women treated with asoprisnil (B).', '1) mm in the asoprisnil/asoprisnil 10-mg (n = 208) and 25-mg (n = 198) groups, respectively (A).', 'The percentages of asoprisnil/asoprisnil 10- and 25-mg women with endometrial thickness 19 mm, as measured by MRI, increased from 13 and 12%, respectively, at day 1 (compared with baseline values of the placebo-controlled phase 3 studies) to 18 and 25% at month 12 of this extension study (24 months total; C).', '\nEndometrial thickness during the extension study (safety analysis population).']","Figure 3 ( ) Mean (SD) values at study visits. ( ) Mean (SD) changes from baseline (start of the placebo-controlled, phase 3 studies) at study visits. ( ) Proportion of women with endometrial thickness 19mm at study visits and at any time during the treatment period. An absence of data indicates that the diagnostic procedure was not performed at that time point. TVU=transvaginal ultrasound.",yes
PMC8555828,Figure_3,oa_package/25/69/PMC8555828.tar.gz,"['In a 90-year-old patient, suffering chronic cardiac failure, we evidenced a deposit of eosinophilic and amorphous material compatible with amyloid within the myocardium ().', '(A) Deposit of eosinophilic and amorphous material compatible with amyloid within the myocardium.']",Figure 3 Deposit of eosinophilic and amorphous material compatible with amyloid within the myocardium. Positive Congo Red histochemical stain. Positive transthyretin immunohistochemistry.,yes
PMC9422426,Figure_2,oa_package/6c/50/PMC9422426.tar.gz,"['Sagittal computed tomography scan slide showing the vidian canal (white arrow) and a pneumatised pterygoid process (black arrow).', 'Axial computed tomography scan slide showing the vidian canal (white arrow) for length measurement.']",Figure 2 Sagittal computed tomography scan slide showing the vidian canal (white arrow) and a pneumatised pterygoid process (black arrow).,yes
PMC8373119,Figure_1,oa_package/0c/bc/PMC8373119.tar.gz,"['S1A and , A and B) (20, 21).', 'Super-resolution images showed that Celsr3 is colocalized with both the presynaptic marker (Bassoon) and the postsynaptic marker (PSD-95) (B and movie S1).', 'Using the same strategies, Frizzled3 was shown colocalized with Bassoon, and Vangl2 was found colocalized with PSD-95 (B and movies S2 and S3).', 'Using 3D STORM, we found that Ryk is present on both the pre- and postsynaptic sides (B and movie S4).', 'Thus, the PCP components maintain their expression and display similar subcellular localization in both developing and adult glutamatergic synapses (C).', 'Localization of Wnt/PCP signaling components in glutamatergic synapses in adult hippocampus and role of Celsrs and Vangl2 in synapse maintenance.', 'We found that knocking out Celsr2 and Celsr3 significantly reduced the glutamatergic synapse numbers (colocalized) in the stratum radiatum (, D to F).', 'Knocking out Celsr2 and Celsr3 significantly reduced the number of the dendritic spines of the CA1 pyramidal neurons (, G and H).', 'S2D) and then analyzed the synapse numbers 2 months later (I).', 'We observed a 27% increase of synapse numbers in Vangl2 cKO by constaining with synaptic markers (, J and K).', 'We found that conditionally knocking out Vangl2 led to an increase of dendritic spines of the CA1 pyramidal neurons 2 months later (, L and M), suggesting that similar to development, Vangl2 also negatively regulates synapse numbers in adult mice.', 'PCP components are localized in glutamatergic synapses in a similar fashion to the asymmetric epithelial cell-cell junctions during PCP signaling (, B and C) (6).']","Fig. 1 Localization of Wnt/PCP signaling components in glutamatergic synapses in adult hippocampus and role of Celsrs and Vangl2 in synapse maintenance. ( ) Schematic diagram showing the hippocampal areas. ( ) Representative 3D STORM images showing the localization of PCP proteins and Ryk (red) with synaptic markers. ( ) Schematic of the distribution of Wnt/PCP components in glutamatergic synapses. mo, months. ( ) Experimental design of viral injection. ( and ) Representative images and quantification of synaptic puncta in the stratum radiatum (SR) (circles indicate colocalization). = 4 animals in each group. ( and ) Representative images and quantification of dendritic spine density. Thirteen dendrites from three control animals and 10 dendrites from four animals with Celsr2/3 sgRNAs. ( ) Experimental design of viral injection. ( and ) Representative images and quantification of synaptic puncta in the SR. = 5 control animals; = 3 Vangl2 cKO animals. ( and ) Representative images and quantification of dendritic spine density. Twenty-one dendrites from three control animals and 17 dendrites from four cKO animals. Students test. * < 0.05, ** < 0.01, and *** < 0.001. Scale bars, 100 nm (B) and 1 m (E, G, J, and L). Error bars represent SEM.",yes
PMC6391965,Figure_8,oa_package/a1/69/PMC6391965.tar.gz,['The NMR derived urine individual metabolic phenotype and its stability over time.'],"Figure 8 The NMRderived urine individual metabolic phenotype and its stability over time. a)Multiple urine samples collected from 12 healthy donors (each identified by a given color) over a period of 20days occupy a welldefined portion of the metabolic space (PCACA score plot), thus indicating that intraindividual variations are much smaller than interindividual differences. This is due to an invariant part of the metabolome characteristic of each individual, which identifies the individual phenotype. b)The individual phenotype over the time scale of 10years is very stable in the absence of physiopathological conditions that can cause abrupt deviations (subjects AG, AW, BD). If this condition is over, the individual phenotype reverts back (AG, AW); adapted from Ref. .",yes
PMC10827543,Figure_1,oa_package/32/5c/PMC10827543.tar.gz,"['Contrast-enhanced CT showing a middle mediastinal tumor (arrow).', ""For CT-PNB, this tumor usually needs to be punctured through the right lung; however, there was concern about the high risk of severe pneumothorax or systemic air embolism due to the long puncture distance in the lung and the patient's history of pulmonary emphysema.""]",Fig. 1 Contrast-enhanced CT showing a middle mediastinal tumor (arrow).,yes
PMC10374041,Figure_4,oa_package/f6/6f/PMC10374041.tar.gz,"['H E staining showed epidermal hyperplasia and accumulation of inflammatory cells in the dermis of the allergen-patched groups compared to the PBS group (A).', 'Repeated patching with either native or recombinant allergens on the skin was able to induce significant epidermal thickness (B).', 'Moreover, all of the aeroallergen-treated groups showed a significant increase in total cells, eosinophil, and neutrophil counts compared to the PBS control group (C).', 'g004(A) H E staining, (B) epidermal thickness of skin lesions and (C) the cell counts of infiltrating inflammatory cells from each group.']",10.1371/journal.pone.0289138.g004,yes
PMC8712948,Figure_4,oa_package/68/75/PMC8712948.tar.gz,[],"Figure4 Expression of Car9 and Eno1 is reduced in proximal tubules expressing shNdufa4l2. Immunofluorescence of Car9 (red, , Eno1 (red, , Ndufa4l2 (red, , GFP (green, marking the expression of shNdufa4l2), and cell nuclei (blue). Staining of TRACK kidneys is shown to the left, and heat-maps of staining intensities in the TANdu kidneys is shown to the right. Panels show representative immunostainings of kidney cortices from three independent mice of each genotype. Magnification; x200, scale bar; 100 m. Representative images are shown. The antibodies used are listed in .",yes
PMC11531661,Figure_4,oa_package/e0/24/PMC11531661.tar.gz,[],"FIGURE 4 Therapeutic effects of mesenchymal stem cellderived extracellular vesicles in preclinical studies of inflammatory bowel disease. Illustration of the mechanisms of mesenchymal stem cellderived extracellular vesicles and their therapeutic effects on inflammatory bowel disease in preclinical studies, achieved by suppressing proinflammatory cytokines and increasing the levels of antiinflammatory mediators.",yes
PMC11200790,Figure_7,oa_package/34/ff/PMC11200790.tar.gz,"['The injection of hPDLSCs are expressed in the skin of mice to PLAP-1 positive cells (A), but no PLAP-1 expression in the control group (B).', 'Fluorescence in situ hybridization of PLAP-1.']","Figure 7 Fluorescence in situ hybridization of PLAP-1. The red channel represents PLAP-1, the blue channel represents DAPI, and the yellow arrows represent PLAP-1 positive cells. There are several PLAP-1 positive cells in the hPDLSCs group, while none in the control group.",yes
PMC8656234,Figure_5,oa_package/02/88/PMC8656234.tar.gz,"['Soluble ULBP4 Increases CD8+ T Lymphocyte Motility and Enhances Kinapse-Like BehaviorsTo evaluate the impact of soluble ULBP4 on human CD8+ T lymphocytes entering the CNS, we used our established live imaging cocultured human astrocyte- CD8+ T lymphocyte assay (, A and B, Videos 1 4, eMethods, The patient s mentation continued to deteriorate.']",Figure 4. Computed tomography angiography of the head and neck showing central venouscatheter tip in the carotid (arrow).,yes
PMC11552350,Figure_9,oa_package/48/11/PMC11552350.tar.gz,"[' 9a).', ' 9a).', ' 9b), which could however not account for the 2.', ' 9c).', ' 9d).', '\nSenescence enhances regulated release of small CD63-positive EVs.', 'n = 3 5 experiments\nDiscussionAn increase in MC numbers with age has been noted in multiple organs, including the skin [11, 64], mesenteric lymphatic vessels [65], the gut [32], the brain [32], and the liver [66].']","Fig. 9 Senescence enhances regulated release of small CD63-positive EVs. ( ) Representative dot blot and densitometric quantification of EVs released by untreated (UT) or IgE/Ag-activated 210 p16 inducible or control BMMCs following a short-term incubation with or without DOX and staining for CD63. ( ) Representative dot blots and densitometric quantification of cell lysates of UT or IgE/Ag-activated p16 inducible BMMCs following a short-term incubation with or without DOX and staining for CD63 and with anti-total ERK2 for normalization. , Nanoparticle Tracking Analysis of EVs concentration and size ( ) or concentration of 30150nm sized EVs ( ) released by 210 IgE/Ag-triggered p16 inducible BMMCs following a short-term incubation with or without DOX. Data is presented as meansSEM. Statistical significance was determined by either Welchs ( ) or (un)paired t-test ( ). =35 experiments",yes
PMC4611013,Figure_2,oa_package/26/7a/PMC4611013.tar.gz,"[' 2).', 'Axial T2-weighted turbo spin-echo MRI showing anal sphincter measurements in a 49-year-old woman.', '2 mm)The observers evaluated the identification of the IAS and EAS as being adequate (moderate-to-good) or inadequate (poor) on the basis of the possibility of evaluating the separate structures of the IAS and EAS.']",Fig. 2 Axial T2-weighted turbo spin-echo MRI showing anal sphincter measurements in a 49-year-old woman. total sphincter thickness (9.4mm); hyperintense intersphincteric fat (1.2mm),yes
PMC8425141,Figure_3,oa_package/95/a1/PMC8425141.tar.gz,"[' 3a, b).', 'CTA (a, b) performed on postoperative day 14 demonstrated a 7.', 'There was hypoperfusion of the superior, anterior, and lateral portions of the right kidney indicating renal infarctCTA (a, b) performed 3 months after angioembolization showed stable size of aneurysm sac.']","Fig. 3 CTA ( , ) performed on postoperative day 14 demonstrated a 7.5cm aneurysm with no contrast enhancement on arterial or delayed phases of imaging. There was hypoperfusion of the superior, anterior, and lateral portions of the right kidney indicating renal infarct",yes
PMC8611279,Figure_9,oa_package/92/c2/PMC8611279.tar.gz,"['9 In particular, the minute structure of the apex was captured in detail ().', '.']","Figure 9. Effects of narrow collimation in intraoral radiography. . Reprinted from Effect of narrow collimation on the image representability of periapical bone defects, by Nishiyama S., 1977; 17:27, .",yes
PMC7660797,Figure_8,oa_package/0f/fb/PMC7660797.tar.gz,['The regulatory effect of NULP1 (nuclear localized protein 1) on cardiac hypertrophy is dependent on nuclear factor of activated T cells 3 (NFAT3).'],"Figure 8 The regulatory effect of NULP1 (nuclear localized protein 1) on cardiac hypertrophy is dependent on nuclear factor of activated T cells 3 (NFAT3). , Left, representative images of immunofluorescence staining for actinin (green) and DAPI (blue) in cultured neonatal rat ventricular myocytes (NRVMs) infected with adenovirus Adsh alone or in combination with Adsh in response to PBS (phosphate buffer saline) Ang II (angiotensin II, 1mol/L) stimuli for 48hours. Scale bar, 20m. Right, relative cell size of cultured NRVMs in the indicated groups. n>50 cells per group. , quantitative polymerase chain reaction (qPCR) analyses of the mRNA levels of atrial natriuretic peptide ( ) and in the indicated groups. n=3 samples per group. , Left, representative images of immunofluorescence staining for actinin (green) and DAPI (blue) in cultured NRVMs infected with Ad alone or in combination with Ad followed by 48hours of PBS or Ang II treatment. Scale bar, 20m. Right, relative cell size of cultured NRVMs in the indicated groups. n>50 cells per group. , qPCR analyses of the mRNA levels of and in the indicated groups. n=3 samples per group. For all statistical plots, the data are presented as the meanSD; in ( through ) ** <0.01; the statistical analysis was performed using a 1way ANOVA. In ( and ) the dotted line indicates the mRNA levels in AdshRNA PBS or AdVector PBS group, which were normalized to 1.",yes
PMC9251334,Figure_2,oa_package/60/57/PMC9251334.tar.gz,"['We also compared the levels of eGFR, creatinine, and blood urea nitrogen (BUN) at 24, 48, and 72 h post-PCI (, Table 4).', 'The levels of blood urea nitrogen (BUN), Serum creatinine (Scr), eGFR and Cystatin C (CyC) at 24, 48 and 72 h post-PCI.']","Figure 2 The levels of blood urea nitrogen (BUN), Serum creatinine (Scr), eGFR and Cystatin C (CyC) at 24, 48 and 72 h post-PCI. BUN; Scr; eGFR; CyC. User group: Black bars, = 242; Nonuser group: Gray bars, = 242. Data were shown as Mean SEM. Student's -test for variables were used. * < 0.05; ** < 0.01, N.S, not significant.",yes
PMC8425141,Figure_2,oa_package/95/a1/PMC8425141.tar.gz,"[' 2a).', ' 2b).', 'Right renal angiogram (a) revealed a 7 cm aneurysm.', 'The inferior pole branch of the renal artery was preserved which allowed perfusion to a majority of the right kidneyThe patient presented for outpatient follow-up on postoperative day 14 with new symptoms of nausea, abdominal pain, night sweats, and chills.']",Fig. 2 Right renal angiogram ( ) revealed a 7cm aneurysm. A superior pole branch of the right renal artery directly fed into the aneurysm. Selective coil embolization ( ) excluded the inflow and outflow tracts for the aneurysm within the superior pole branch of the right renal artery. The inferior pole branch of the renal artery was preserved which allowed perfusion to a majority of the right kidney,yes
PMC7699768,Figure_9,oa_package/33/a2/PMC7699768.tar.gz,"['3D volume rendering images provide clear views of the stent location in relation to the coronary anatomy as shown in .', '3D volume rendering images of 3D printed coronary models with stents placed in the coronary arteries.']",Figure 9 3D volume rendering images of 3D printed coronary models with stents placed in the coronary arteries. Reprinted with permission under open access from Sun and Jansen [ ].,yes
PMC11327010,Figure_3,oa_package/6f/16/PMC11327010.tar.gz,"['Furthermore, SMS could suppress iNOS and IL-18 mRNA levels in a dose-dependent manner (F).']","FIGURE 3 SMS exhibited no cytotoxicity in macrophages and downregulated the expression of LPS-induced pro-inflammatory mediators. RAW264.7 macrophages were treated with SMS at concentrations ranging from 0.03 to 8mg/mL in the absence or presence of LPS (1g/mL) for 24h. Cell viability was determined by MTT assay. RAW264.7 and peritoneal macrophages were pre-treated for 3h with the indicated concentrations of SMS and further incubated for 24h with or without LPS (1g/mL) before assessment of NO production and iNOS protein expression by Griess assay and Western blotting respectively. Representative immunoblot results and their quantifications are shown. -actin was used as an internal loading control. RAW264.7 macrophages were pre-treated with SMS at the indicated concentrations and further incubated for 24h with or without LPS (1g/mL) before assessment of IL-6 and IL-1 secretion by ELISA. Effect of SMS on the mRNA expression of iNOS, IL-1, IL-18, and IL-6. Data are expressed as means SD of three independent experiments. * compared to LPS-induced control.",yes
PMC8603796,Figure_3,oa_package/11/08/PMC8603796.tar.gz,"['Biopsy taken from the left retromolar region demonstrated proliferation of large cells with abundant coarsely granular eosinophilic cytoplasm [a-c] that stained positive with periodic acid Schiff [b].', 'Photomicrograph of tissue section from the left retromolar region showing stratified squamous epithelium with pseudoepitheliomatous hyperplasia (a-c black arrow).']","Figure 3 Photomicrograph of tissue section from the left retromolar region showing stratified squamous epithelium with pseudoepitheliomatous hyperplasia (a-c black arrow). The granules were periodic acidSchiff positive (b); large granular cells with abundant coarse cytoplasm and round nucleus (a-c blue arrow) (hematoxylin and eosin stain, 40)",yes
PMC5154308,Figure_8,oa_package/d4/d9/PMC5154308.tar.gz,"['In addition, this screw is inserted in such a way that the risk of hardware exposure and soft tissue healing complications is minimized ().', 'Midfoot Fusion Bolt technique minimal incision for arthrodesis followed by the insertion of 6,5 mm screws; X-ray postoperative aspect of the foot and surgical illustration (MFB Synthes DePuy)Another frequently applied technique involves the percutaneous bone shaving as a minimally invasive approach that can remove the plantar osseous prominence through a small incision that is away from the plantar weight-bearing area.']","Fig. 8 Midfoot Fusion Bolt technique minimal incision for arthrodesis followed by the insertion of 6,5 mm screws; X-ray postoperative aspect of the foot and surgical illustration (MFB Synthes DePuy)",yes
PMC4526196,Figure_1,oa_package/73/3f/PMC4526196.tar.gz,"[' 1(b)) showed that steatosis was developed and numerous lipid droplets were observed in all of the five livers from the NAFLD rats.', '01, compared to positive control group (6w)H E staining for histological evaluation.', '(Magnification 400x)In the following 6 weeks, rats of high-dose group and middle-dose group showed significantly reduced body weight (by 5.', ' 1d, e, f).', ' 1 and 2).', ' 1 and 2).']",Fig. 1 H&E staining for histological evaluation. Typical photographs of liver sections of normal rat ( ) and NAFLD rat ( ) in 4weeks; ( )normal rat (negative control group); ( ) the rat treated with TFSC (100mg/kg) for 6weeks; ( ) the rat treated with TFSC (200mg/kg) for 6weeks; ( ) the rat treated with TFSC (400mg/kg) for 6weeks; ( ) positive control group. (Magnification 400x),yes
PMC3668729,Figure_3,oa_package/5f/82/PMC3668729.tar.gz,"['Mutant SOD1 Astrocytes Globally Release a Lower Amount of Proteins but a Larger Proportion of Proteins through ExosomesIn the conditioned media from WT and mutant SOD1 astrocytes, G93A SOD1 astrocytes globally secreted 43% fewer proteins than WT SOD1 astrocytes (A), confirming an impairment of protein secretion.', 'In the subsequent differential analysis of the secretome of G93A SOD1 and WT SOD1 astrocytes by two-dimensional gel electrophoresis-based proteomics, surprisingly, the protein patterns of the two conditions were very similar except for six spots (B).', 'However, the increasing total levels of mutant SOD1 and VCP/p97 in astrocyte-conditioned media were confirmed by slot blot (, C and D).', 'In agreement with the results of the unprocessed conditioned media (A), the decrease in the amount of proteins in the corresponding G93A SOD1-derived exosome-depleted fractions was maintained (', '6ea Spot numbers (see B).']","FIGURE 3. , media were collected from similar numbers of cells expressing WT or G93A SOD1, and secreted proteins were prepared as described for slot blot experiments and quantified by BCA protein assay. Protein quantification was performed in at least seven samples per group. Each sample was a 3-well pool. Values are the amounts of secreted proteins normalized to total cell proteins (secreted proteins/total cell proteins) and are means S.E. ( ). An indicates statistical significance ( < 0.05), Student's test. , for two-dimensional gel electrophoresis maps ( = 3 for each genotype), the same amount of secreted proteins (30 g) were loaded on IPG strips (pI 47). The gel maps were stained with Sypro Ruby and compared by computerized image analysis. Six proteins were differentially secreted by astrocytes expressing mutant SOD1 and controls. refer to proteins listed in . and , slot blot and relative quantification of SOD1 ( ) and VCP/p97 ( ) released by the astrocytes confirmed the proteomic analysis and showed that the total level of the extracellular proteins were higher than in control conditions. The astrocytes were plated in 6-well dishes, and equal volumes of conditioned media from 3-well pools were used for the slot blot analysis. Values are immunoreactivities and are means S.E. ( = 3) normalized to WT, set as 100. An indicates statistical significance ( < 0.05), Student's test.",yes
PMC11556475,Figure_8,oa_package/e9/f0/PMC11556475.tar.gz,"['aam_89_24-F8"">Anterior view of left kidney showing Unilateral hydronephrosis of renal pelvis existing with unilateral accessory renal arteries.']","Figure 8 Anterior view of left kidney showing Unilateral hydronephrosis of renal pelvis existing with unilateral accessory renal arteries. Red arrows = Hydronephrosis of renal pelvis, Black arrow = Accessory renal arteries",yes
PMC10714945,Figure_8,oa_package/ce/1d/PMC10714945.tar.gz,"['2 Molecular docking analysisFirst, molecular docking simulation was performed between verapamil and BSA (Table 1; ).', 'The site displayed a single polar contact with the BSA particle, involving just LYS, at position 136 (Table 1; Supplementary Table S1; ).', '0 kcal/mol, respectively) (Table 2; ).', ', 2020; Oso and Olaoye, 2020) (Table 2; Supplementary Table S2; ).', 'Of particular note is LYS-136, which forms a polar contact with the strongest binding affinity (Table 1; Supplementary Table S1; ).', 'It is the only one having an RMSD 3 (Table 2; Supplementary Table S2; ).']","FIGURE 8 Visualization of verapamil docking sites (modes 1) in bovine serum albumin (BSA) as well as in glycosidases: -amylase (A), -glucosidase (G), and sucrase-isomaltase (SI). The spatial structure of verapamil has been marked in cyan color. ASN, asparagine; HIS, histidine; LYS, lysine.",yes
PMC4558819,Figure_5,oa_package/8d/64/PMC4558819.tar.gz,['The nerve bundles immunostained with S-100 protein [].'],Figure 5 Immunostaining of nerve bundles with S-100 protein (200),yes
PMC8339820,Figure_3,oa_package/ff/fb/PMC8339820.tar.gz,['Posteromedial view of the left idiopathic congenital clubfoot of a fetus aborted after the 25th week of gestation.'],Figure 3 Posteromedial view of the left idiopathic congenital clubfoot of a fetus aborted after the 25 week of gestation. The talar neck is inferiorly angulated in contact with the sustentaculum tali. (I) Tibiotalar ligament; (II) Talocalcaneal interosseous ligament. (With kind permission from: Windisch G. Anatomical Study for an Updated Comprehension of Clubfoot. J Child Orthop 2007;1:79-85).,yes
PMC11618033,Figure_4,oa_package/75/b0/PMC11618033.tar.gz,"['Mice were treated with Berb (100 mg/kg body weight, orally), RDV (15 mg/kg body weight, intraperitoneally) or vehicle (1% DMSO in PBS) starting 12 h post infection ( 4A).', 'By 6 dpi, the vehicle and RDV groups showed similar body weight losses of over 15% ( 4B).', 'In contrast, Berb-treated mice experienced only 5 6% body weight loss and showed significant body weight recovery ( 4B).', 'The survival rate in the Berb-treated group was superior to that of RDV-treated group ( 4C).', 'Gross lung morphology changes and hemorrhage scores were consistent with the observed body weight and survival differences ( 4D).', 'However, Berb and RDV administration had a limited effect on viral loads ( 4E).', ' 4Therapeutic treatment with Berb decreased the viral load of SARS-CoV-2 and reduces disease severity in mice(A) The pictorial diagram shows the therapeutic treatment in a mouse model.', 'Tg infected mice (s 4F and 4G).', 'Histopathological examination revealed that vehicle-treated mice exhibited immune cells infiltration, in the alveoli and alveolar walls ( 4H).', 'In contrast such infiltration is markedly reduced in Berb-treated mice ( 4H).', 'Immunological analysis showed extensive SARS-CoV-2 N-antigen staining in lungs of vehicle-treated mice ( 4I), whereas N-antigen staining was significantly reduced in Berb-treated animals ( 4I).']","Figure3 Berb retains antiviral activity against Alpha, BA.1, BA.2, BA.5, and XBB.1.16 Mice were intranasally inoculated with 110 PFU of Alpha, and Omicron variants (BA.1, BA.2, and BA.5). The mice were treated with Berb (100mg/kg) or vehicle (0mg/kg) b.i.d. from the time of inoculation (0hpi) to 5 dpi ( = 5 for each group). (A and B) Percentage change in weight ( = 5) and survival ( = 5) was measured in Alpha infected and Berb-treated mice (two-way ANOVA followed by Tukeys multiple comparison test; <0.0001). (CF) Percentage change in weight (BA.1, BA.2, and BA.5) and survival (Wilcoxon test) was measured in BA.5 infected and Berb-treated mice. (G) Percentage change in weight was measured in XBB.1.16 infected and treated mice. The bar representsSEM. two-way ANOVA followed by Tukeys multiple comparison test (body weight); <0.0001. (HK) The image shows gross lung morphological changes, and the bar graph represents lung hemorrhage score. 05, where 0 is a normal pink healthy lung, and 5 is a diffusely discolored dark red lung (black arrow indicates color of the lungs). Bar graph representsSEM. One-way ANOVA followed by Tukeys multiple comparison test; <0.05, <0.005, <0.0005, <0.0001. (L and M) Virus titers in the lungs were measured by plaque assay. The values shown are means SEM. Statistical significance was measured by one-way ANOVA followed by Tukeys multiple comparison test; <0.05, <0.005, <0.0002. (N) Lung sections were subjected to hematoxylin and eosin staining, and the bar graph illustrates histology scores as mentioned previously. The images were captured at 40 magnification, with a scale bar of 100m. Two-way ANOVA followed by Tukeys multiple comparison test. Bar graph representsSEM. See also .",yes
PMC11307019,Figure_3,oa_package/2a/bd/PMC11307019.tar.gz,['Retuned synapses after MAO-B blocking by deprenyl in 6-month-old AppNL-F mice.'],"Fig. 3 Pre-treatment of hippocampal slices with the MAO-B blocker deprenyl significantly decreases resting membrane potential in pyramidal cells in mice (A). Blocking MAO-B increased both frequency (B) and amplitude (C) of mEPSC. Representative traces are shown under graphs. Student -test and Mann-Whitney test: <0.01; <0.001 statistically significant as shown. +aCSF: =8 cells recorded in four animals; +deprenyl: =8 cells recorded in four animals. The subthreshold theta-burst ( -burst) stimulation induced long term potentiation in both treated and untreated slices from mice (D). Representative traces are shown on top. When we compared the average fEPSP magnitude at 05min and 5560min after stimulation, the potentiation was significantly lower after deprenyl pre-treatment (E, F). Mann-Whitney test: <0.01, <0.001 statistically significant as shown. +aCSF: =6 recordings in four animals, +deprenyl: =5 recordings in three animals.",yes
PMC6055824,Figure_1,oa_package/f7/1d/PMC6055824.tar.gz,['Somatic inactivating CTNNA1 mutations correlate with invasive lobular and ducto lobular mixed type breast cancer.'],"Figure 1 Somatic inactivating mutations correlate with invasive lobular and ductolobular mixedtype breast cancer. (AD) H&E processed samples harboring an inactivating mutation and nonmutated alleles. Examples of a case diagnosed as ILC (A) and two cases that were initially diagnosed as IDCNOS, but that presented ductolobular features (B, C) are shown. The case shown in D represents a typical IDCNOS and was diagnosed based on histological features and a positive Ecadherin IHC analysis. Cancer studysample ID, amino acid change (somatic mutation), and mRNA levels are indicated in the given order. The inset depicts initial (i) and revised (r) diagnosis. Samples can be crossreferenced with Table ; data were obtained from . Scale bar=100m.",yes
PMC8403418,Figure_8,oa_package/ce/ef/PMC8403418.tar.gz,"['8a, b).', ', not significantThe effect of A11 on cerebral vascular permeabilityA11 is a humanized scFv Ab that preferentially binds to activated platelets and can lyse platelet thrombi [36].']","Fig. 8 Increased loss of neurons in the hippocampus of HFD-treated 3 Tg mice. Representative immunohistochemical images of neurons at different hippocampal subregions stained with anti-NeuN antibody. Scale bar, 50 m. Quantification of the numbers of NeuN-positive neurons at different hippocampal subregions ( = 3/group). Data are expressed as mean SD, unpaired Student test, two-tailed. < 0.01; < 0.001; n.s., not significant",yes
PMC7431833,Figure_6,oa_package/84/1c/PMC7431833.tar.gz,[],"FIGURE 6 Schematic diagram depicting the possible mechanisms. In AD pathology, the imbalance of the APP metabolic pathway caused the abnormally accumulation and aggregation of A in the brain, and the abnormal increase in the activity and expression of BACE1 is one of the most important causes for the A accumulation in AD. Our study found that the inhibition of SIRT2 induces ubiquitination and degradation of RTN4B by deacetylating RTN4B, then the upregulation of RTN4B leading to the reduction of BACE1, suppress A production, ultimately alleviates the cognitive decline of AD.",yes
PMC4268619,Figure_1,oa_package/c8/44/PMC4268619.tar.gz,"['Chest radiography showed cardiomegaly and dilation of the main PA and its branches [].', 'Chest X-Ray pulmonary artery view showing cardiomegaly and dilatation of the main pulmonary artery and right descending pulmonary arteryApical 4C view showing moderate tricuspid regurgitation and severe pulmonary arterial hypertension (pulmonary artery systolic pressure, PASP = 84 mm Hg)(a) Parasternal short axis view at the level of the pulmonary artery showing aneurysmal dilatation of the main pulmonary artery (measuring 66 mm), (b) The corresponding images in a are shown in 3D-echocardiography showing markedly dilated main pulmonary artery and the bifurcation areaColor doppler imaging in parasternal short axis view at the level of the pulmonary artery showing severe pulmonary regurgitationFor evaluation of pulmonary vasculature, the patient underwent a computed tomographic angiography showing an aneurysm affecting the main PA [].']",Figure 1 Chest X-Ray pulmonary artery view showing cardiomegaly and dilatation of the main pulmonary artery and right descending pulmonary artery,yes
PMC6074404,Figure_2,oa_package/ae/09/PMC6074404.tar.gz,"['The GelSeal cap was removed while the skin protector remained to extract the specimen and to protect the skin from tumor contamination ().', 'Bowel specimen extracted from the umbilical port with skin protector in place.']",Figure 2 Bowel specimen extracted from the umbilical port with skin protector in place.,yes
PMC7990648,Figure_1,oa_package/a9/39/PMC7990648.tar.gz,"['The cyst had remained stable until two years before the referral ().', '.']",Figure 1. Health checkup images two years before the referral. Abdominal US (a) and MRCP (b) showing a unilocular cyst at the pancreas body without MPD dilation.,yes
PMC11546136,Figure_3,oa_package/f1/e6/PMC11546136.tar.gz,"['Duplex ultrasound investigation of the right subclavian vein revealed limited inflow ().', 'Procedural images for a middle-aged patient with right UEDVT secondary to an indwelling peripherally inserted central catheter who underwent mechanical thrombectomy.', 'Case #4An 81-year-old male inpatient was referred to Interventional Radiology by the Hospitalist for treatment of right UEDVT secondary to PICC line and a symptom duration of 2 days.']","Fig. 3 Procedural images for a middle-aged patient with right UEDVT secondary to an indwelling peripherally inserted central catheter who underwent mechanical thrombectomy. The (A) prethrombectomy duplex ultrasound of the right upper extremity revealed limited flow into the subclavian vein and (B) prethrombectomy venogram confirmed extensive thrombosis from distal basilic vein to the subclavian vein. As shown on the (C) post-thrombectomy venogram, thrombectomy was completed with >90% thrombus removal and restoration of inline flow.",yes
PMC9582891,Figure_5,oa_package/4e/1f/PMC9582891.tar.gz,"['RCM of the Dermal-Epidermal Junction (DEJ).', 'The papillary and superficial reticular dermis display blood vessels and collagen fibers.']","Figure 5 RCM of the Dermal-Epidermal Junction (DEJ). (A) , characterized by elongated hyper-reflective cords, corresponding the junctional thickening. (B) Higher magnifications showing enlongated cords interconnecting, round white structures represent the milia-like cysts (red arrows). (C) Histopathology showing the enlargement of the interpapillary space, formed by aggregated melanocytes, with predominantly small, interconnecting nests at the tip of the rete ridges.",yes
PMC6993764,Figure_3,oa_package/a0/46/PMC6993764.tar.gz,[],Figure 3 Full thickness skin graft with Panniculus carnosus muscle partially removed,yes
PMC6010669,Figure_2,oa_package/cb/e6/PMC6010669.tar.gz,"['The day after the patient developed a respiratory insufficiency because of a hydro-pneumothorax (), therefore a chest tube was inserted in the left hemithorax and he was transferred to the Intensive Care Department for hemodynamic and respiratory monitoring and stabilisation.', '']","Fig. 2 Chest X-ray on day 3, showing a hydro-tensionpneumothorax of the left lung.",yes
PMC5342343,Figure_8,oa_package/72/52/PMC5342343.tar.gz,['Binge ethanol exposure-induced endoplasmic reticulum (ER) stress in the pancreasMice were exposed to binge ethanol for 10 days as described in the Materials and Methods.'],"Figure 8 Binge ethanol exposure-induced endoplasmic reticulum (ER) stress in the pancreas Mice were exposed to binge ethanol for 10 days as described in the Materials and Methods. Six hours after last ethanol exposure, mice were euthanized and the pancreatic tissues were processed by immunoblotting analysis of ER stress markers The relative protein expression of ATF6 , GRP78/BIP , p-PERK , p-eIF2 and CHOP was quantified and normalized to -tubulin. Each data point was the mean SEM of three independent experiments. * denotes statistical difference < 0.05) and ** denotes significant difference ( < 0.01) from the control.",yes
PMC11456794,Figure_2,oa_package/19/44/PMC11456794.tar.gz,"['After the intervention, the patient had stable hemodynamics, passed yellow stools, and was discharged hospital after 3 days ().', 'DSA images.', ':DiscussionConventional treatment options for lower gastrointestinal bleeding include endoscopic hemostasis, transcatheter arterial embolization, and surgery.']",Fig. 2 DSA images. (A) Active bleeding site at the hepatic flexure of the colon (white arrow). (B) Selecting and occluding the lesion with 01 Interlock detachable coil 3 mm 15 cm (red arrow). (C) Scan shows complete occlusion of the lesion (yellow arrow).,yes
PMC7200202,Figure_2,oa_package/42/30/PMC7200202.tar.gz,"['A tangential biopsy found granulomatous inflammation associated with refractile yeast compatible with cryptococcosis, and a positive mucicarmine stain corroborated the diagnosis ().', 'Histopathologic evaluation of punch biopsy specimen from the left lateral upper eyelid.']","Fig 2 Histopathologic evaluation of punch biopsy specimen from the left lateral upper eyelid. , Hematoxylin-eosin stain; original magnification: 20. , Positive mucicarmine stain supporting the diagnosis of cutaneous cryptococcosis. (Original magnification: 40).",yes
PMC10600767,Figure_5,oa_package/e0/cd/PMC10600767.tar.gz,['\nHistopathological image.'],Figure 5 A: Case 1; B: Case 2.,yes
PMC3042671,Figure_11,oa_package/6e/62/PMC3042671.tar.gz,[],Figure 11 Photomicrograph of H&E-stained section from a submucosal lipoma in the colon. The tumor is composed of mature fibroadipose tissue with sharp demarcation from the overlying mucosa.,yes
PMC8160561,Figure_25,oa_package/9c/f7/PMC8160561.tar.gz,[],Fig.25 Histopathologically proven splenic sarcoidosis: 36-year-old male patient with new-onset low-grade fever and cough. Axial plane postcontrast abdominal CT image shows multiple hypodense nodular lesions in both the liver and spleen (arrowheads). Note was also made of multiple enlarged lymph nodes in the paracaval and paraaortic regions (arrows),yes
PMC10689292,Figure_4,oa_package/cf/1d/PMC10689292.tar.gz,[],FIGURE 4 Schematic representation showing intestinal duplication (reproduced from Ganesh (11)).,yes
PMC10602148,Figure_3,oa_package/3e/c9/PMC10602148.tar.gz,"['2a protects striatal DA fibers loss in Syn miceTo test the impact of 2a on Syn-induced loss of DA neurites projecting to the striatum, we measured the density of TH + terminals in the striatum of brain sections (A E).', '0001, C E) compared to control AAV-empty vector injected mice treated with saline (', '3A E) and compared to AAV-empty vector mice treated with 2a (B E).', '001, D E).', '2a protects against loss of striatal DA and DA metabolites in Syn miceWe analyzed levels of DA and its metabolites including 3,4-dihydroxyphenylacetic acid (DOPAC), 3-methoxytyramine (3-MT) and homovanillic acid (HVA) in the ipsilateral striatal hemisphere of AAV-A53T- Syn mice compared to the control AAV-empty vector mice treated with saline or 2a (F I).', '0078, F) and by about 50% compared to AAV-empty mice treated with 2a (**p 0.', '004, F).', '0068, F).', '034, G) and HVA (*p = 0.', '0147, I) with only a non-significant trend for 3-MT (', 'We found a significant increase in striatal serotonin (5-HT) upon administration of 2a (compared to saline) in AAV-empty mice, but no effect of 2a versus saline on 5-HT levels in the AAV-A53T- Syn injected mice (J).', 'No effects from AAV-A53T- Syn or from 2a treatment were seen for striatal norepinephrine (NE, K).', '2), and attenuates loss of striatal DA fibers (A E) and striatal monoamines (', 'Treatment with 2a protects against loss of striatal DA fibers and monoamines in AAV-A53T- Syn injected mice:A-D) TH immunohistochemistry in representative striatal sections of male mice treated with 2a (10 mg/Kg for 100 days) at 120 days post-injection of AAV-A53T-SNCA, scale bar is 500 mm.']","Figure 3 Treatment with 2a protects against loss of striatal DA fibers and monoamines in AAV-A53T-Syn injected mice: A-D) TH immunohistochemistry in representative striatal sections of male mice treated with 2a (10 mg/Kg for 100 days) at 120 days post-injection of AAV-A53T-SNCA, scale bar is 500 mm. E) Relative optical density of TH+ fibers in the ipsilateral striatum compared with the contralateral side of male mice in each group (n=68 mice per group). Significance determined by one-way ANOVA. Error bars represent mean SD F-K) Evaluation of ipsilateral striatal monoamine in mice treated with 2a (10 mg/Kg for 100 days) or saline solution at 120 days post-injection of AAV-A53Tor AAV-empty as control; F) mean DA levels, G) mean of 3,4-Dihydroxyphenylacetic acid levels (DOPAC), H) mean levels of 3-methoxytyramine (3-MT), I) mean levels of homovanillic acid (HVA), J) mean levels of 5-hydroxytryptamine (5-HT) and K) mean levels of Norepinephrine (NE); the values are expressed as fold change (79 mice per group). Significance determined by one-way ANOVA. Error bars represent mean SD.",yes
PMC6803513,Figure_2,oa_package/12/7e/PMC6803513.tar.gz,"['The previously significant associations between COM-Mem and COM-EF with FA and MD were attenuated well below significance in all instances, except for COM-EF and FA (see and Table 3 for a summary of critical values).', 'Positive associations between COM-EF and white matter, based on fractional anisotropy (FA) at 90% threshold, depicted in radiological view.']","Figure 2 Positive associations between COM-EF and white matter, based on fractional anisotropy (FA) at 90% threshold, depicted in radiological view. Mean FA skeleton shown in green. Areas of significant association shown in red.",yes
PMC9698469,Figure_4,oa_package/9a/1e/PMC9698469.tar.gz,"['The results of the observation of VEGF expression using the Kruskal Wallis test analysis in the negative control group, the implant group, the implant + oral BHA group, and the implant + oral calcium lactate group at 7, 14, and 28 days (b) showed different values.', '(a) Immunohistochemistry of VEGF expression (400 magnification).']","Figure 4 ( ) Immunohistochemistry of VEGF expression (400 magnification). VEGF-positive cells are indicated by brown osteoblasts (red arrows); ( ) differences in IRS values of VEGF expression, each bar graph represents IRS SD. The sign (*) indicates < 0.05 with the MannWhitney Test.",yes
PMC3995393,Figure_2,oa_package/01/3b/PMC3995393.tar.gz,['a HE-stained biopsy section showing deposition of a basophilic amorphous material in the upper and middle dermis.'],Fig. 2 HE-stained biopsy section showing deposition of a basophilic amorphous material in the upper and middle dermis. Note also the granulomatous infiltration surrounding the amorphous substance and accumulation of collagen. A high-power view showing histiocytic infiltrates intermingled with lymphocytes and giant cells between collagen fibers. von Kossa staining confirmed that the amorphous substance was calcium deposition. Magnification: 40; 200; 40.,yes
PMC9342776,Figure_3,oa_package/25/d9/PMC9342776.tar.gz,"['Trpc6E896K/E896K animals developed greater albuminuria after 2 weeks compared to wild-type animals, but this difference did not persist at the end of the infusion period (A).', 'Serum creatinine did not differ between genotypes either at baseline, or at the end of the experiment (B).', 'g003Effect of Trpc6 genotype on glomerular response to angiotensin II infusion.', 'g003"" position=""float""/>Histologic examination of kidney sections revealed rare glomerular lesions and isolated tubular dilations with proteinacious casts (C).', 'Although there was a trend toward more glomerular lesions in Trpc6E896K/E896K mice, this did not reach statistical significance (D).', 'Podocyte density was lower in angiotensin II treated animals compared to controls (E), but Trpc6 genotype did not affect this parameter.', 'The effect was driven by an increase in average glomerular cross-sectional area (F), with no evidence of significant podocyte loss (G).']",10.1371/journal.pone.0272313.g003,yes
PMC4330237,Figure_4,oa_package/b9/9b/PMC4330237.tar.gz,"[' 4.', 'A systematic approach helps analysis and takes place with the assessment of following:Main real hard tissue, soft tissue and air shadows in a PTG: 1, condylar process; 2, coronoid processes; 3, ramus; 4, angle; 5, body; 6, parasymphysis area; 7, symphysis area; 8, foramen mentale; 9, submandibular fossa; 10, mandibular canal; 11, linea oblique externa; 12, foramen mandibulae; 13, cortical border of the mandible; 14, glenoid fossa; 15, articular surfaces of the temporal bone; 16, articular eminence; 17, zygomatic arch; 18, a, b, c anterior and posterior cortical boundaries and floor of the maxillary sinus; 19, pterygomaxillary fissure; 20, maxillary tuberosity; 21, hamulus; 22, orbital rim; 23, infraorbital canal; 24, body of zygoma; 25, temporozygomatic fissure; 26, anterior nasal spine; 27, floor of the nasal cavity; 28, inferior nasal concha; 29, foramen incisivum; 30, hard palate; 31, external auditory meatus; 32, body of the cervical vertebra; 33, hyoid bone; 34, soft palate; 35, nasopharyngeal air shadow; 36, ear lobe\nEntire radiograph: The entire radiograph must be overviewed to assess the developmental stage of the dentition, developmental stage of a single tooth and location of each tooth/tooth follicle, tooth eruption, and possible supernumerary and missing teeth (']","Fig. 4 Main real hard tissue, soft tissue and air shadows in a PTG: 1, condylar process; 2, coronoid processes; 3, ramus; 4, angle; 5, body; 6, parasymphysis area; 7, symphysis area; 8, foramen mentale; 9, submandibular fossa; 10, mandibular canal; 11, linea oblique externa; 12, foramen mandibulae; 13, cortical border of the mandible; 14, glenoid fossa; 15, articular surfaces of the temporal bone; 16, articular eminence; 17, zygomatic arch; 18, a, b, c anterior and posterior cortical boundaries and floor of the maxillary sinus; 19, pterygomaxillary fissure; 20, maxillary tuberosity; 21, hamulus; 22, orbital rim; 23, infraorbital canal; 24, body of zygoma; 25, temporozygomatic fissure; 26, anterior nasal spine; 27, floor of the nasal cavity; 28, inferior nasal concha; 29, foramen incisivum; 30, hard palate; 31, external auditory meatus; 32, body of the cervical vertebra; 33, hyoid bone; 34, soft palate; 35, nasopharyngeal air shadow; 36, ear lobe",yes
PMC8788985,Figure_1,oa_package/e3/b3/PMC8788985.tar.gz,[],"FIGURE 1 Microphotograph. Crosssection of a benign canine prostate, which is characterized by a bilobed structure and densely packed glandular tissue. Haematoxylin and Eosin (HE) stain. Size bar indicates 4mm",yes
PMC11660520,Figure_9,oa_package/ab/16/PMC11660520.tar.gz,[],"FIGURE 9 Examples of determinants of ApoE immunopositivity in amyloid plaques. (a) Three small (<10m) diffuse amyloid plaques with no evidence of ApoE immunopositivity. (b) A single diffuse amyloid plaque (<10m) with a trace ApoE immunopositivity without overlapping DAPI positivity. (c) A bit larger (>10m) ostensibly diffuse amyloid plaque that is strongly ApoE positive. However, a strong excitation that already saturates the nuclei, reveals a central nidus of fibrillar amyloid that overlaps with the strongest ApoE signal.",yes
PMC11548950,Figure_1,oa_package/bb/27/PMC11548950.tar.gz,"['().', '24305252Demyelination and remyelination of the early MS lesion as revealed by neuropathological studies [1, 2].']","Figure 1 Demyelination and remyelination of the early MS lesion as revealed by neuropathological studies [ , ]. Oligodendrocytes experience cell death and are unable to maintain the myelin sheath; then, activated microglia or monocyte-derived macrophages digest (scavenge) the dead oligodendrocytes and dysfunctional myelin without damaging the axons. Thereafter, oligodendrocyte precursor cells (OPCs) become the new oligodendrocytes and remyelinate the axons, restoring functionality.",yes
PMC6925519,Figure_2,oa_package/0d/1d/PMC6925519.tar.gz,"[' 2.', '2).', '2a).', '2b-d) and using the HUSPIR ratio which examines NMDAR2B degradation that occurs postmortem [6] (Additional file 8: S2).', '2e) confirms preservation of pre and postsynaptic terminals in pairs with our synaptoneurosome preparation as we had previously observed [49].', 'Enrichment and integrity analysis of synaptic protein preparations a) A representative western blot from 3 cases shows the enrichment of synaptic proteins and exclusions of histones from the synaptoneurosome preparation (P) compared to crude homogenate (H) protein from that sample.', 'Scale bar represents 500 nmHaving confirmed that the extracted protein is of appropriate quality, we then applied a comprehensive workflow to enable us to assess (at the protein level) the relative contribution of both regional vulnerability and APOE variants as a risk factors to AD pathogenesis (']","Fig. 2 Enrichment and integrity analysis of synaptic protein preparations ) A representative western blot from 3 cases shows the enrichment of synaptic proteins and exclusions of histones from the synaptoneurosome preparation (P) compared to crude homogenate (H) protein from that sample. Blots were probed for PSD95, synaptophysin, histone and GAPDH. Total protein analysis (TPA) was also used to determine whether any samples showed evidence of protein degradation. Boxes in panel ( ) indicate the molecular weight ranges analysed for total protein stain. Quantification reveals no difference in total protein in BA17 ( ) or BA41/42 ( ) samples (One way ANOVAs, >0.05). Transmission electron microscopy confirms that synaptoneurosome preparations contain paired pre and post synaptic terminals as expected ( ). We observe clear electron dense postsynaptic densities (arrows), presynaptic vesicles (arrowheads), presynaptic mitochondria (m) and small processes associated with synapses (*). Scale bar represents 500nm",yes
PMC9557788,Figure_1,oa_package/0f/1c/PMC9557788.tar.gz,['.'],"Figure 1. Screening mammography detected left breast abnormality in a 45-year-old woman. (A) Grayscale ultrasound showed a well-defined oval shaped hypoechoic lesion (arrow) at 2 oclock position in the left breast having partially indistinct margins. It was categorized as a BI-RADS category IVa with low suspicion of malignancy. (B) By strain elastography, the mass was graded with a visual score of 2 according to the Tsukuba system (arrow). (C) Strain ratio was 0.6. (D) By shear wave elastography, the mass was graded with a visual score of 2. (E) KPa value was 43. Ultrasound guided core biopsy confirmed the lesion to be a fibroadenoma.",yes
PMC7548444,Figure_6,oa_package/b9/9a/PMC7548444.tar.gz,"['Tumor weight, measured after necropsy, showed that tumors of control untreated mice reached a weight of 522,6 100 mg while T2 treatment (5 and 25 mg/kg) significantly decreased tumor weight ( 6A).', 'In accordance, the average number of mitotic figures in tumors of T2 treated mice was lower than in control ones ( 6B).', ' 6In vivo treatment with T2 inhibited 4T1 tumor growth, invasion and metastasis.', '5% of T2-treated ones (5 and 25 mg/kg, respectively) had this ability ( 6C).', 'However, in T2 treated mice tumors, there was less evidence of invasion with less expansive edges and collagen fibers appeared more straight and aligned parallel to the tumor border ( 6D).', 'With respect to blood dissemination, the T2 treated mice developed a significantly lower number of spontaneous lung metastases per mouse compared with control untreated mice ( 6F).', 'Supp Supp Table 1 e) (a) anatomical surface markings (b) sub-mandibular incision given (c) swelling exposed and dissection done (d) specimen retrieved in toto (e) gross specimen,yes
PMC11394960,Figure_11,oa_package/1d/8f/PMC11394960.tar.gz,[],Figure 11 Inflammation profiles in response to infection of both the GI.1 and GI.2 genotypes.,yes
PMC10544238,Figure_7,oa_package/cd/42/PMC10544238.tar.gz,"['Blood glucose levels during pyruvate tolerance and glycerol tolerance tests were significantly lower in Mc4r-KO HKO mice than in Mc4r-KO F/F mice (, A and B), but these differences were smaller than those between ob/ob HKO and control mice (, A and B), suggesting that hepatic FASN deficiency suppresses gluconeogenesis in Mc4r-KO mice but to a lesser extent than it does in ob/ob mice.', 'This suppression was associated with higher FFA and lower -hydroxybutyrate levels in plasma in fasted Mc4r-KO HKO mice than in control mice (C), but again these differences were smaller than those apparent for ob/ob HKO and control mice (H), suggesting that hepatic FASN deficiency suppresses FAO to a lesser extent in Mc4r-KO mice than in ob/ob mice.', 'Unexpectedly, Mc4r-KO HKO mice, unlike ob/ob HKO mice, manifested neither a reduced level of expression for genes related to FAO, gluconeogenesis, or glycolysis (D) nor enhanced AMPK activation, as assessed by phosphorylation of AMPK and its substrates (E), in the liver, but they showed markedly augmented hepatic insulin signaling (F) compared with Mc4r-KO F/F mice.', 'Consistent with the lack of exacerbation of fed hyperglycemia in Mc4r-KO HKO mice, the hepatic citrate level in these mice was identical to that in Mc4r-KO F/F mice (G).', 'Hepatic FASN deficiency in Mc4r-KO mice improves glucose metabolism by inhibiting gluconeogenesis and augmenting insulin signaling.']","Figure 7 Hepatic FASN deficiency in Mc4r-KO mice improves glucose metabolism by inhibiting gluconeogenesis and augmenting insulin signaling. ( and ) Pyruvate ( ) and glycerol ( ) tolerance tests for 20-week-old Mc4r-KO F/F and Mc4r-KO HKO mice ( = 7). ( ) Plasma FFA and -hydroxybutyrate levels in fasted 16- to 20-week-old mice ( = 6). ( ) RT-qPCR analysis of the expression of PPAR target genes related to FAO or ketogenesis as well as of genes related to gluconeogenesis or glycolysis in the liver of fasted 16- to 20-week-old mice ( = 6). ( ) Immunoblot analysis of phosphorylated and total forms of AMPK subunits, ACC, and RAPTOR in the liver of fasted 16- to 20-week-old mice ( = 5 or 6). ( ) Effects of insulin on IR and Akt phosphorylation in the liver of 16- to 20-week-old mice. Mice deprived of food overnight were injected intravenously with insulin (5 U/kg) or PBS (), 2 minutes after which the liver was isolated, lysed, and subjected to immunoblot analysis. Each lane corresponds to 1 mouse, and the blots are representative of 2 independent experiments. ( ) Hepatic citrate levels in 20-week-old Mc4r-KO F/F and Mc4r-KO HKO mice in the fed state ( = 5). All quantitative data are means + SEM for the indicated numbers of mice. * < 0.05, ** < 0.01 compared with Mc4r-KO F/F mice or as indicated (2-tailed Students test).",yes
PMC2835709,Figure_2,oa_package/93/30/PMC2835709.tar.gz,['Postoperative lateral radiograph of the patient.'],Figure 2 .,yes
PMC7487709,Figure_4,oa_package/8e/28/PMC7487709.tar.gz,"[' 4a, b).', ' 4a, b).', ' 4c), supporting previous findings [11].', ' 4d).', '', 'd co-localisation of TDP-43/TBPH and PolyGA aggregatesPan-neuronal expression of DPRs leads to neurodegeneration and cell death in the Drosophila central brainIn order to establish whether expression of DPRs in the Drosophila nervous system resulted in hallmarks of neurodegeneration histological analysis was performed.']","Fig.4 TDP-43/TBPH mislocalisation in DPR expressing TDP-43/TBPH (magenta) and DPR (green) localisation in Salivary glands. Scale bars 50m boxed region expanded in ( ). Quantification of the percentage of TDP-43/TBPH localising to the nucleus or cytoplasm (60 cells from 3 animals, from 3 independent crosses, per genotype). Quantification of the percentage of DPR containing cells with insoluble TDP-43/TBPH inclusions. Total number of DPR containing cells (n) are shown on bars, total number of animals (N)=3 per genotype. co-localisation of TDP-43/TBPH and PolyGA aggregates",yes
PMC11239145,Figure_3,oa_package/b1/26/PMC11239145.tar.gz,['Preparation and sterilization of the operative field anew.'],Figure 3 Preparation and sterilization of the operative field anew. The operative field was sterilized again before the patient was repositioned in a supine position.,yes
PMC10285468,Figure_2,oa_package/21/24/PMC10285468.tar.gz,"['After these treatments, her right shoulder remained inflamed ().', 'Keloids.']","Fig 2 Keloids. Patients shoulders following bilateral excision, grafting, and radiation. Patients failing radiation with active inflammation, tenderness, and pruritus.",yes
PMC9723401,Figure_2,oa_package/93/1f/PMC9723401.tar.gz,[],"FIGURE 2 Radiographic evaluation. (A) Cone beam computed tomography showing perforation into the maxillary sinus (arrow). (B,C) T1weighted MRI showing a vascular lesion of the palate extending into bone (arrows).",yes
PMC7829733,Figure_1,oa_package/22/73/PMC7829733.tar.gz,['\nComparison of ultrasonography and magnetic resonance images in case of the abscess.'],Figure 1 A: The infected space showing the anechoic area with the surrounding wall. B: T2 axial-weighted magnetic resonance images with high signal intensity showing the involvement of buccal space.,yes
PMC10828587,Figure_1,oa_package/ae/2e/PMC10828587.tar.gz,"['Clinical photo of left frontotemporal superficial mass on initial presentation, approximately 2 months postinjury.', 'Unenhanced computed tomography of the head was obtained to rule out traumatic etiology such as the rare possibility of an undiagnosed skull fracture and subsequent leptomeningeal cyst.']","Fig. 1 Clinical photo of left frontotemporal superficial mass on initial presentation, approximately 2 months postinjury. The mass was firm, painless, and mobile with an overlying reddish discoloration.",yes
PMC11412541,Figure_5,oa_package/e6/82/PMC11412541.tar.gz,"['ref047"" ref-type=""bibr"">47], we co-cultured HTR-8 cells and M0-type macrophages derived from THP-1 cells to imitate cell-cell interaction at the maternal-fetal interface (A, 5D and 5G).', 'Notably, TgAg failed to directly affect the migratory and invasive capacity of trophoblast cells (B and 5E).', 'In addition, TgAg had no impact on the proliferation of trophoblast cells in the absence of macrophages (H).', 'g005TgAg inhibits trophoblast cell migration, invasion, and proliferation via affecting macrophages.', 'Likewise, the addition of HSP60 to TgAg-treated macrophages can significantly improve the inhibited biological functions of trophoblast cells (C, 5F and 5I).', 'Our data showed that Trem2 knockdown in THP1 cells further promoted the inhibitory effect of TgAg on HTR-8 migration and invasion (J and 5K).']",10.1371/journal.ppat.1012543.g005,yes
PMC6090953,Figure_5,oa_package/a1/b0/PMC6090953.tar.gz,[' 5).'],"Fig. 5 Immunohistochemical profile of the tumour after orbital exenteration: The tumor cells express pancytokeratin AE1/AE3 , EMA , SMA ( ) and express focally S100 protein ( )",yes
PMC10545525,Figure_8,oa_package/9d/5c/PMC10545525.tar.gz,"['We confirmed the overexpression of MTM1-CS in the analysed muscles (Supplementary A).', 'Upon histological analysis, MTM1-WT expression was found to increase fibre size and improve mitochondria position in Bin1mck / muscle (A C).', 'At the ultrastructural level, we saw a general improvement of myofibre ultrastructure and notably a correction of the number of T-tubules per sarcomere (D-E).', '\nLate MTM1 overexpression ameliorates histological hallmarks of Bin1 KO mice.']","Figure 8 . ( ) Representative images of the two contralateral tibialis anterior (TA) muscles from the same animal stained by haematoxylin and eosin and succinate dehydrogenase (SDH). Scale bar = 50 m. ( ) MinFeret diameter repartition of TA fibres between large (40 m) and small (<40 m) categories ( 5). ( ) Percentage of fibres presenting SDH accumulation in their centre ( 3). ( ) Representative longitudinal electron microscopy pictures of contralateral TA from 12-week-old mice. Scale bar = 500 nm. ( ) Quantification of the number of T-tubules per sarcomere ( = 3, 24). Ratio paired -test, unpaired -test with Welch's correction, nested one-way ANOVA followed by Fisher's LSD test, * < 0.05, ** < 0.01, *** < 0.001. KO = knockout; WT = wild-type.",yes
PMC5562695,Figure_6,oa_package/be/9f/PMC5562695.tar.gz,"['Illustration of a clinical registration case.', '\nMSERg and SSERg 7 illustrates a frequency plot showing in how many instances either the SSERg or MSERg registration yielded the best result among clinical (N = 25) and synthetic (N = 25) testing sets.']","Figure 6 Illustration of a clinical registration case. ( ), ( ) are the fixed and moving images with landmarks annotated by the pathologist and the radiologist. The tumor ROI annotations on the pathology image are made by the pathologist and the corresponding tumor ROI on the MRI are mapped from pathology annotations obtained via manual registration. ( ), ( ) show the registration results of intensity-based registration; ( ), ( ) of MACMI, ( ), ( ) of MSERg and ( ),( ) of SIFT, respectively. The light blue lines in panels ( ) illustrate the tumor ROI on a T2-weighted MRI slice and the magenta lines highlight the corresponding ROI on the transformed pathology image. ( ) illustrate the landmark alignment results where the cyan colored landmarks represent those identified on the MRI, while the yellow colored landmarks represent those corresponding locations on the transformedpathology image. The dark blue lines in ( ) illustrate the capsule boundary on MRI.",yes
PMC5594473,Figure_1,oa_package/8f/b5/PMC5594473.tar.gz,"['CT arteriography (CTA) was used preoperatively to show the tumors segmental arteries [7] ( 1).', '4 (4 6)\nCTA was used to position tumors and their corresponding segmental arteries.', 'Superior branch supplied the lesions in the upper pole (A1) and anterior branch supplied the lesions in the lower pole (A2)\nSurgical methodsPatients were administered general anesthesia and placed in the lateral decubitus position.']","Fig. 1 CTA was used to position tumors and their corresponding segmental arteries. CTA showed the tumors in the upper (T1) and lower pole (T2) of right renal; Superior, anterior and posterior branch were separated from the right renal artery near the renal hilum. Superior branch supplied the lesions in the upper pole (A1) and anterior branch supplied the lesions in the lower pole (A2)",yes
PMC8909604,Figure_1,oa_package/d0/23/PMC8909604.tar.gz,"['Examples of LA:Ao and LAAPD measurements performed by different observers are presented in .', '1585032644291LA:Ao and LAAPD performed by different observers.']",Figure 1 LA:Ao and LA performed by different observers. The first row shows LA:Ao measurements of the same dog performed by different observers. The second row shows LA measurements of the same dog performed by different observers. O1: veterinary student; O2: first-year cardiology resident; O3: third-year cardiology resident; Crd1: cardiologist 1; Crd2: cardiologist 2.,yes
PMC10190694,Figure_1,oa_package/5f/47/PMC10190694.tar.gz,['\nHistology of small intrahepatic bile ducts.'],Figure 1 A: Septal bile duct lined by a columnar epithelium supported by a fibrous wall; B: Interlobular bile duct (arrow) lined by cuboidal epithelia. Haematoxylin and eosin; bar corresponds to 50 m.,yes
PMC8527176,Figure_4,oa_package/90/2b/PMC8527176.tar.gz,"[', following depolarization or NMDAR stimulation), Pyk2 accumulates in the nucleus where it can interact with partners identified in non-neuronal cells (MBD2, p53) and in postsynaptic densities (PSDs) in dendritic spines (one is magnified in the dotted circle), where it can bind to PSD-95, SAP102 or SAPAP3 (see text for references and ).', ', 2005), providing a basis for its enrichment near NMDAR ().']","FIGURE 4 Pyk2 recruitment and activation in post-synaptic densities. When intracellular Ca increases, Ca /calmodulin (CaM) interacts with the PSD-95 region between its SH3 and guanylate kinase-like (GK) domains ( ; ). This makes the SH3 domain available for interaction with PR2 or PR3 motifs in Pyk2 and facilitates its enrichment in PSDs. Pyk2 can also interact with other PSD proteins (SAP102, SAPAP3) but the regulation and dynamics of these interactions have not been investigated. The binding of Ca /calmodulin to PSD-95 favors dimerization of PSD-95 and clustering and activation of Pyk2, which is in the vicinity of NMDAR. Ca /calmodulin can also bind directly to and activate Pyk2 (not shown, see ). Pyk2 and the associated SFKs, presumably mostly Fyn and Src, phosphorylate NMDAR on tyrosine. One consequence of tyrosine phosphorylation of NMDAR is to increase its surface expression. The main phosphatase which opposes these effects by dephosphorylating tyrosine residues on Pyk2, SFKs, and NMDAR is STEP. STEP is inhibited by phosphorylation by cAMP-dependent protein kinase (PKA) and indirectly activated by the Ca -activated phosphatase calcineurin.",yes
PMC5641992,Figure_3,oa_package/04/e5/PMC5641992.tar.gz,"[' 3A).', '3B).', '\nA: A 41 is deposited in leptomeningeal blood vessels.', 'Scale bars = 100 m (a d) and 500 m (e l)\nA 38 and A 41 deposit in vessels prior to AD onsetWe analyzed the accumulation of A 41 in contrast to A 38, A 40, and A 42 in the brain at various stages of SP.', '3C).', ' 3A, Additional file 1: Table S1), both modulation of -secretase activity and failure of A drainage could be at cause for A accumulation/deposition and CAA in AD brains [33].', '3C, Additional file 1: Table S1).']","Fig. 3 : A41 is deposited in leptomeningeal blood vessels. Frozen sections from an AD brain were subjected to the immunostaining using antibodies against A41 (green in ), A42 (red in ), and A40 (red in ). Double immunostaining against both A41 and A42 demonstrated that the anti-A41 antibody labeled the arterioles (#) in the subarachnoid space, but not the senile plaques (*) in the parenchyma ( and ). Double staining against A41 and A40 is shown. Three different stages of amyloid angiopathies, with weak or no A41 deposition (arrowhead), modest A41 deposition (thin arrow), and with severe A41 deposition (thick arrow) are shown ( and ). A41 was found in the amyloid angiopathy with severe A40 deposition. Contrary to the A40, which deposited in the periphery of adventitia, A41 seemed to be localized in the smooth muscles layer of blood vessels. Scale bars=50m. : Time course of in vitro A aggregation. Each synthetic A incubated and measured thioflavin T fluorescence. A142 aggregates immediately compared to the other variants. A140 and A141 were similar and showed little aggregation characteristic for 24h. : IHC for As accumulation in the occipital cortex sections. A38 and A41 deposited in the leptomeningeal blood vessels in aged SP free brain (NO.9) ( , ). As38, 40, and 41 also deposited in AD (NO.4) and CAA brains (NO.3), but A40 had little deposits in the cortex, including arterioles ( , ). Amount of A42 deposited in subpial granular cell layers and cortex ( , ). Scale bars=100m ( ) and 500m ( )",yes
PMC7149979,Figure_12,oa_package/55/dc/PMC7149979.tar.gz,[],"Fig. 19.12 Myelin oligodendrocyte glycoprotein (MOG) antibody-associated demyelinating encephalomyelitis. A demyelinating lesion associated with MOG antibodies is well demarcated ( , luxol fast blue) and shows relatively well-preserved ( , CNPase; , MOG) oligodendrocytes and deposition of C9 neoantigen ( , arrows). 200.",yes
PMC7026874,Figure_4,oa_package/06/fc/PMC7026874.tar.gz,['A) Mediolateral oblique and B) craniocaudal views of mammogram in an 82-year-old femaleLarge lesion with spiculated margins in the upper outer quadrant of right breast consistent with a neoplastic lesion (arrows).'],Figure 4 A) Mediolateral oblique and B) craniocaudal views of mammogram in an 82-year-old female Large lesion with spiculated margins in the upper outer quadrant of right breast consistent with a neoplastic lesion (arrows). A well-defined rounded density adjacent to this suspicious lesion was a cyst as correlated on ultrasound.No right axillary lymphadenopathy was noted on mammogram.,yes
PMC4423270,Figure_2,oa_package/31/64/PMC4423270.tar.gz,"['The pathology results indicated mucositis with extensive erosion and the presence of a predominantly neutrophilic inflammatory infiltrate with degeneration and apoptosis of basal layer keratinocytes ().', '.']",Figure 2. Predominantly neutrophilic inflammatory infiltrate with hydropic degeneration of the basal layer and apoptotic keratinocytes.,yes
PMC5525253,Figure_2,oa_package/01/d7/PMC5525253.tar.gz,['p Capillary proliferation in fibrovascular core of papilla\nStatistical analysisAll of the aforementioned parameters were evaluated in two paired comparison groups (i.'],Fig. 2 (2) Further representative images of histological parameters evaluated in this study. Example of a nuclear groove (arrow). Prominent nucleoli. Whorling pattern. Single spotty necrosis (arrow). Multifocal group necrosis (arrows). Surface necrosis. Confluent necrosis. Glandular differentiation. Squamous differentiation. Micropapillary differentiation. Mitosis level 1. Mitosis level 2. Mitosis level 3. Apoptosis score 1. Apoptosis score 3. Capillary proliferation in fibrovascular core of papilla,yes
PMC3032862,Figure_2,oa_package/b2/bc/PMC3032862.tar.gz,"[' 2).', '', '\nBy conventional radiography, an acute rib fracture can be identified by a linear lucency in the bone.']","Fig.2AB ( ) An AP chest radiograph of a 3-month-old infant shows a classic posteromedial rib fracture near the midline (arrow), with other healing rib fractures seen elsewhere (arrowheads). ( ) An axial CT image of the same patient shows the same posteromedial fracture with irregular linear lucency (arrow). It is more clearly evident on the CT image than on the radiograph shown in ( ). Other rib fractures with periosteal reaction indicating healing are also present (arrowheads and curved arrow).",yes
PMC6635342,Figure_1,oa_package/31/11/PMC6635342.tar.gz,"['Examination identified soft digitate mucous colored papules on the vestibule and inner aspect of both labia minora (a).', ':(a); Multiple fine, pink papillary projections, located into the inner aspect of the labia minora.', 'The findings were consistent with a diagnosis of vulvar vestibular papillomatosis (b).']","Fig. 1: Multiple fine, pink papillary projections, located into the inner aspect of the labia minora. There are thickening and hyperplasia of the nonkeratinizing squamous epithelium rich in glycogen, overlying central fibrovascular core (H&E 10)",yes
PMC7723932,Figure_1,oa_package/fe/d6/PMC7723932.tar.gz,"[' 1).', 'Representative examples of staining results for selected antibodies in tumor tissues.', 'Bar indicates 200, 100 and 50 m in the first, second and third line, respectivelyGenerally, PIDT provided good to optimal results regarding staining intensity.']","Fig. 1 Representative examples of staining results for selected antibodies in tumor tissues. Bar indicates 200, 100 and 50m in the first, second and third line, respectively",yes
PMC5526151,Figure_1,oa_package/33/77/PMC5526151.tar.gz,"['01; ).', 'Western blot analysis revealed that the protein expression levels of phosphorylated p70S6K in the hippocampal primary neurons of SAMP8 mice were significantly increased when compared with the control SAMR1 group, indicating enhanced mTOR activity in aged mice ().', '403721923553.']","Figure 1. Upregulation of the mTOR pathway in aged SAMP8 compared with SAMR1 . Western-blot analysis showed the protein expression levels of mTOR (pSer2448) and p70S6K (pThr389) of aged SAMP8 mice and neurons derived from SAMP8 mice were significantly increased when compared with SAMR1 mice. P<0.01 vs. SAMR1 group. mTOR, mammalian target of rapamycin; SAMP8, senescence accelerated mouse prone 8; SAMR1, senescence accelerated mouse resistant 1. SAMP8 group, brain tissue of SAMP8; SAMR1 group, brain tissue of SAMR1; neurons-SAMP8 group, neurons of SAMP8; neurons-SAMR1 group, neurons of SAMR1.",yes
PMC8704563,Figure_2,oa_package/9d/a3/PMC8704563.tar.gz,"['Raji cells, which express the Fc RII receptor and a little Fc RIII receptor (A), have been shown as a good model for testing SARS-CoV-1 induced ADE in vitro [11].', 'The qRT-PCR results showed that the serum greatly enhanced viral infection in a time-dependent manner, similar to what has been observed in primary B cells (B).', 'Similarly, more sgRNA was observed in the serum treated group, suggesting more replicating viruses in the ADE group (B).', 'The qRT-PCR results showed that at least four of the convalescent sera induced ADE, although the effect was not associated with RBD antibody titer or neutralizing titer (C).', 'Notably, ADE effect was most obvious in a medium level of 1:80-dilution and decreased following dilution, which was similar to ADE in dengue viral disease (D).', 'In comparison, two serum samples that were known to induce slight or strong ADE in were used.', 'Factors related to ADE effect.']","Figure 2 Factors related to ADE effect. ( ) The expression of FcRs in Raji B cells, a cloned immortalized cell line. ( ) Raji B cells were infected with SARS-CoV-2 at a moi of 0.01, 0.1, and 0.2 with or without convalescent sera. Samples were harvested at 0 h, 24 h, or 48 h post infection and cellular viral load was then quantified by qRT-PCR detection of total viral RNA or subgenomic RNA (sgRNA). ( ) SARS-CoV-2 virus pretreated with equal-volume convalescent sera from different COVID-19 patients (1:40 of final dilution) or RPMI1640 at 37 C for 30 min. Viral mixtures were added to Raji B cells at a moi of 0.1. Samples were harvested at 48 h post infection and cellular viral load was then quantified by qRT-PCR detection of sgRNA. RBD IgG OD value and neutralizing titer of convalescent sera were quantified by ELISA or micro-neutralization assay, respectively. ( ) Convalescent serum from one COVID-19 patient was diluted to 1:40, 1:80, 1:160, and 1:320. SARS-CoV-2 was pre-incubated with equal-volume sera at 37 C for 30 min and then added to Vero E6 or Raji B cells at a moi of 0.1. Samples were harvested at 48 h post infection and cellular viral load was then quantified by qRT-PCR detection of viral RBD. The Vero E6 detection data can be found in . Comparison between different sample groups was analyzed by the Students -test. * <0.05; ** < 0.01; *** < 0.001; NS, no significance. The short lines mean to compare viral load in infected cells with or without patient sera).",yes
PMC5043008,Figure_3,oa_package/33/ff/PMC5043008.tar.gz,"[' 3a t = 12 h).', ' 3a and Sup.', ' 3a t = 30 and Sup.', ' 3b, arrow).', '', 'See also supplemental Movie 1 and 2\nDynamic properties of GFP GFAP are different from GFP GFAP To assess the dynamic properties of GFAP and GFAP , FRAP experiments were performed on U251 astrocytoma cells in which small boxed regions of fluorescent cells were photobleached, and recovery of fluorescence was measured over time.']","Fig.3 Collapse of the IF network due to high GFPGFAP expression. Stills from a representative live cell imaging experiment. U343MG cells were transfected with GFPGFAP and imaged for 48h. The GFPGFAP was initially incorporated into the IF network ( at =12h), but as the amount of GFPGFAP increased over time, it eventually caused a collapse of the network ( =18h). During the process of collapsing, thicker and shorter filamentous structures are visible in the cell, which are moving into the direction of the collapsed network ( in =30h). These small filaments sometimes co-localized with vimentin in U251MG cells as well ( ). represent 20m. See also supplemental Movie 1 and 2",yes
PMC9977427,Figure_3,oa_package/22/04/PMC9977427.tar.gz,"['Mice sensitized with MC903 + OVA (MC903 + OVA/PBS) displayed a trend toward increased frequency of circulating basophils (A), and these basophils expressed elevated levels of the basophil markers Fc RI and IgE (B) when compared with vehicle-sensitized mice (ethyl alcohol [EtOH] + PBS/PBS).', 'Strikingly, ocular OVA challenges (MC903 + OVA/OVA) led to a trend of further increased basophil frequency (A) and upregulation of Fc RI and IgE compared with control mice (B), suggesting that conjunctivitis-associated blood basophils are more responsive to IgE-mediated stimulation.', 'This local basophil infiltrate was rarely seen in PBS-challenged mice, indicating that it was an immune response upon antigen re-exposure in conjunctival tissues (, C and D).', 'These genes include Mcpt8 and Cpa3, 2 protease-encoding transcripts; Fcer1 and Ms4a2, which encode the and chains of the high-affinity IgE receptor; and Cd200r3, which encodes an activating receptor (21, 22) (E).', 'Additionally, the basophil signature transcripts from several genes encoding chemokines (Ccl3, Ccl4, and Ccl9) were highly expressed in OVA-challenged mice (F), implying a role for basophils in recruiting other leukocytes to sites of inflammation.', 'Notably, OVA challenges also induced the expression of genes involved in chemotaxis, such as basophil-directed chemokine Ccl2 and its receptor Ccr2 (F), suggesting that basophils migrate out of circulation via chemotactic activity (23 25) dependent on CCL2 (possibly derived from mast cells and eosinophils).', 'We observed an increase in the mast cell protease-1 (Mcpt1) conjunctival transcript level (G) and serum mouse mast cell protease-1 (mMCP-1) protein expression (H) in OVA-challenged mice, suggesting mast cell degranulation may also contribute to conjunctivitis in this model.', 'Upon exposure to OVA via eye drops, basophils were recruited into inflamed conjunctival tissues and further upregulated activation markers with an enhanced capacity to mediate an antigen-induced reaction in the setting of AD-associated conjunctivitis () (37).', 'Basophils exhibit a distinct phenotype in AD-associated conjunctivitis.']","Figure 3 Basophils exhibit a distinct phenotype in AD-associated conjunctivitis. ( ) Representative flow cytometry plots and frequency of NTNB (CD3e CD19 ) CD49b FcRI/IgE blood basophils in mice on day 24 of the conjunctivitis model. Data depicted are from 1 experiment ( = 510) and are representative of 2 independent replicates. * < 0.05 by 1-way ANOVA with Tukeys post hoc test. ( ) FcRI/IgE expression measured by MFI on blood basophils in mice on day 24 of the conjunctivitis model. Data depicted are from 1 experiment ( = 510) and are representative of 2 independent replicates. ( ) IHC staining performed on conjunctival tissue sections at day 24 with Ab against mMCP-8 (specific for basophils). Upper, MC903 + OVA/PBS; lower, MC903 + OVA/OVA. Scale bar: 100 m. ( ) Basophil IHC scores. Data are from 2 pooled experiments ( = 10). ( ) Heatmap of basophil signature genes. ( ) Heatmap of chemokine and receptor signature genes. The color gradient in and represents fold-change values; OVA-challenged samples were compared with PBS-challenged samples ( = 45). ( ) transcripts in conjunctival tissues ( = 45). ( ) mMCP-1 in serum. Data depicted in , , , and are from 1 experiment ( = 10) and are representative of 2 independent replicates. ** < 0.01, *** < 0.001, **** < 0.0001 by unpaired Students test. NTNB, non-T non-B.",yes
PMC3745117,Figure_5,oa_package/9d/cf/PMC3745117.tar.gz,"['Sometimes the skull may grow in the vertical direction in response to growth along the parieto-temporal suture to give the appearance of a tower and is then known as turricephaly [].', 'The computed tomography scan shows bilateral fused coronal sutureSchematic pathogenesis of turricephaly deformity (upper row).']",Figure 5 Schematic pathogenesis of turricephaly deformity (upper row). The X-ray skull shows thumb printing indicative of raised intracranial pressure. The clinical appearance in a 3-years-old child (lower row),yes
PMC4498558,Figure_2,oa_package/37/2d/PMC4498558.tar.gz,"[' 2).', 'Amyloid-PET on June, 25th, demonstrating the absence of cortical amyloid in all brain regionsDiscussionDue to demographic changes an ever rising number of elderly patients present to Medical Services with cognitive impairment.']","Fig. 2 Amyloid-PET on June, 25th, demonstrating the absence of cortical amyloid in all brain regions",yes
PMC6946576,Figure_1,oa_package/3b/27/PMC6946576.tar.gz,['Complete tear.'],"Figure 1 Complete tear. Acute phase. Ultrasound image showing complete tear of the mid-portion of the plantar fascia (white void arrow), with retraction of the 2 stumps (white arrowheads) and local effusion (asterisk) (A). Corresponding schematic drawing of the complete plantar fascia lesion with the gap-sign and perilesional edema (white dotted line) (B). Follow-up at 2 months. Ultrasound image shows a small fibrous bridge (white void arrow) between the 2 stumps (white arrowheads) and reduction of the local edema (C). Corresponding schematic drawing showing the alignment of the proximal and distal segments of the plantar fascia with a small amount of perilesional edema (white dotted line) (D). FP = fat pad, IM = intrinsic muscles.",yes
PMC3015700,Figure_5,oa_package/6d/ea/PMC3015700.tar.gz,"['The stomach was decompressed (), and the trocar sites in the stomach were closed with 3 0 braided absorbable sutures.', '.']","Figure 5. Ulcer was removed with gastroscope using an endoscopic snare, and the stomach was decompressed.",yes
PMC6196709,Figure_5,oa_package/32/ba/PMC6196709.tar.gz,[],Figure 5 Healed left ankle lesions,yes
PMC9520570,Figure_6,oa_package/64/1c/PMC9520570.tar.gz,"['Ob/ob mice have even more steatosis than intragastric alcohol fed mice (A), which has been well documented (Rull et al.', '05), which was somewhat lower than that observed with alcohol feeding models (B).']","FIGURE 6 Expression of LRP1 and APP in ob/ob steatosis mice. Representative H&E histology (200 magnification) of ob/ob mice liver and control mice (J, NJ) at 20weeks of age. The arrows point to lipid droplets in the liver associated with steatosis. LRP1 and APP expression in the liver of ob/ob with advance steatosis. = 6 mice per group. 6J and 6NJ represent two different control mice for ob/ob mice. Results are mean SD; **** < 0.0001, ** < 0.01, or * < 0.05 vs. J or NJ controls.",yes
PMC5054886,Figure_2,oa_package/e5/49/PMC5054886.tar.gz,['\nACR of murine cardiac allografts and comparison of human and murine array results.'],"Figure 2 . (A) Schema of included groups to study the murine miR expression profile in ACR following HTX. (B) Representative CD45 staining of corresponding regions in control hearts and cardiac allografts, 3d (Allo3d) and 7d (Allo7d) after HTX, with quantification in (C). (D and E) depict CD4 and Mac3 staining, respectively. (F) Heat map of differentially expressed miRs in murine control hearts and failed grafts. (G) Volcano plot showing fold change (log values) and probability (log values) for individual miRs, comparing control hearts and Allo7d. (H) ISH of miR155 during murine ACR showed miR155 staining in inflammatory cells (black arrowheads), whereas adjacent ventricular myocardium didn't stain (white arrowheads). (I) Venn diagrams showing the numbers and overlap of differentially regulated miRs between humans and mice. (J) Fold changes for all miRs in humans and mice during ACR; common dysregulated miRs in humans and mice during ACR are highlighted. (K) Following HTX, splenic SPI1 protein levels markedly decreased, whereas splenic RNA levels significantly increased (L) (*p<0.05). ACR, acute cellular rejection; HTX, heart transplantation.",yes
PMC4192061,Figure_4,oa_package/88/ab/PMC4192061.tar.gz,"['Removal of extracellular Ca2+ caused rapid accumulation of FM dyes at the injury sites indistinguishably for WT or ML1-null myofibers (Supplementary b).', 'In contrast, removal of extracellular Ca2+ significantly increased the entry of FM dyes (Supplementary c), suggesting that the influx of extracellular Ca2+ is essential for repair8.', 'Transient Ca2+ increases were observed at injury sites in the presence or absence of external Ca2+ (Supplementary d g).', 'However, intracellular Ca2+ release was less in ML1 KO myoblasts or in the presence of ML-SI compounds25 (Supplementary f, g), suggesting a contribution to Ca2+ release from lysosomes during membrane damage or early stage of membrane repair.', 'In the presence of external Ca2+, prolonged Ca2+ influx was observed in ML1 KO myoblasts or ML-SI3-treated C2C12 myoblasts (Supplementary d, e).', 'EB-positive muscle cells were more numerous in CTX-treated ML1-null muscle than in CTX-treated WT controls (Supplementary h).', 'In non-muscle cells, including mouse embryonic fibroblasts (MEFs) and bone marrow-derived macrophages (BMMs), SLO treatment caused more PI-positive cells for ML1 KO (a Supplementary ', 'In addition, ML-SI3 increased PI staining in WT, but not KO cells (a Supplementary ', 'Consistently, SLO treatment also increased whole-cell IML1 by 2 3-fold in C2C12 cells (b, c).', 'Consistent with the possibility that overexpression of ML1 promotes membrane repair in mdx muscle4,21, AAV-GFP-ML1-infected muscle exhibited a significant reduction in both EB dye uptake and dystrophic area compared with the contralateral noninjected control muscle (f, g).', 'An essential role of ML1 in lysosomal exocytosis, membrane repair, and protection of muscle damage in vivo(a) C2C12 and MEF cells were treated with pore-forming toxin streptolysin O (SLO; 2 5 g/ml), and stained with PI, a marker for membrane damage.']","Figure 4 An essential role of ML1 in lysosomal exocytosis, membrane repair, and protection of muscle damage ( ) C2C12 and MEF cells were treated with pore-forming toxin streptolysin O (SLO; 25 g/ml), and stained with PI, a marker for membrane damage. FACS quantification of PI staining was performed in cells with or without SLO or external Ca (2 mM). ( ) SLO (0.2 g/ml) treatment increased whole-cell in C2C12 cells transfected with GFP-ML1. SLO (0.5, 1 and 2 g/ml for 15 min) treatment on the release of lysosomal acid phosphatase (AP; determined using an AP activity colorimetric assay kit) into the culture medium in WT and ML1 KO MEFs. The data are presented as the percentage of the activity of the released total cell-associated enzymes. SLO (0.5 g/mL for 15 min) treatment on the release of lysosomal acid SMase (aSMase; determined using an aSMase activity assay kit) in WT and ML1 KO MEFs. ( ) The effect of AAV-GFP-ML1 infection on EB uptake and dystrophic area in mdx Gastroc muscle. Data are presented as the mean s.e.m.",yes
PMC4230564,Figure_3,oa_package/c5/3e/PMC4230564.tar.gz,[],"FIGURE 3. The pioneers of angiography in Slovenia. From left to right, upper row: Uro Vizijak, Marijan Pocajt, Duan Pavnik. Lower row: Sead Galija, Duan Tomai and Joe Matela.",yes
PMC9834923,Figure_3,oa_package/68/28/PMC9834923.tar.gz,"['A\nnon-contrast computerized tomography chest was done and the tip of the PICC was at\nthe junction of the right subclavian vein and the right brachiocephalic vein ().', '1177_2050313X221147421-fig3"" position=""float""/>DiscussionThis case demonstrates a rare case of bilateral TPN pleural effusions as a delayed\ncomplication of PICC placement in an adult patient.']",Figure 3. CT chest showing PICC position (red to blue arrows) crossing paths with dualICD leads inside the right subclavian vein.,yes
PMC8203565,Figure_1,oa_package/ff/24/PMC8203565.tar.gz,"['Unenhanced antemortem CT about 6 h before her death (A and B) showing the loss of gray and white matter differentiation and diffuse brain swelling, which suggested hypoxic ischemic encephalopathy.', 'About 12 h after her death, unenhanced head and whole-body postmortem CT was performed, followed by subsequent body autopsy.']","Fig. 1 Unenhanced antemortem CT about 6 h before her death (A and B) showing the loss of gray and white matter differentiation and diffuse brain swelling, which suggested hypoxicischemic encephalopathy. Subsequent enhanced antemortem CT (C and D) showed no abnormal enhancement of the brain parenchyma. Postmortem CT (E and F) 12 h after the patient's death showed unexpected and symmetrical hyperdense lesions of the bilateral caudate nuclei and putamina (arrows). The loss of gray and white matter differentiation and diffuse brain swelling was continued to be observed.",yes
PMC11233829,Figure_2,oa_package/94/7e/PMC11233829.tar.gz,"['CT revealed enlarged, serpiginous vascular structures on the radial side of his right forearm as well as soft tissue edema in the right hand ().', 'Contrast-enhanced MR angiography of the right hand.']","Fig. 2 Contrast-enhanced CT of the right forearm and right hand in the venous phase. (A, B) Coronal images of the right arm (A) and right hand (B) showed enlarged vessels on the radial side of the patient's right forearm and right hand (arrows). Several enlarged lymph nodes on the medial side of the patient's right upper arm were observed (arrowheads in A). (C) Axial view of the right hand showed extensive soft tissue edema (arrowheads).",yes
PMC7110585,Figure_4,oa_package/5c/83/PMC7110585.tar.gz,"['Aggregates of organisms are found rarely in or around tubules as small, basophilic structures (', '']","Fig.4 microbes are within the tubular epithelial cells and these are sloughing into the lumen of the tubules. The black arrows indicate intratubular and intraepithelial densely packed microbes, which are small round/oval amphophilic structures. Samples from a dwarf rabbit ( ) (HE stain, 40 magnification).",yes
PMC6040270,Figure_3,oa_package/37/7c/PMC6040270.tar.gz,"['We also showed that an increase in lipid droplet accumulation is spontaneously accelerated in the liver tissue of DKO mice at an early stage of life, due to an impaired triglyceride-rich lipoprotein secretion (s 3 and 4), which was associated with heightened oxidative stress in the livers caused by the combined deprivation of SOD1 and Prdx4 ().', '002""/>Lipid accumulation in livers of DKO mice.']","Figure 3 Lipid accumulation in livers of DKO mice. (a) Representative images of Oil Red O-stained livers with the indicated genotypes. Scale bars: 50 m. (b) Plasma TG levels in mice with the indicated genotypes. Data are expressed as the mean SEM. Number of mice: WT: = 5, Sod1 : = 7, Prdx4 : = 10, and DKO: = 6. < 0.05 versus WT mice. (c) Plasma NEFA levels in mice with the indicated genotypes. Data are expressed as the mean SEM. Number of mice: WT: = 5, Sod1 : = 5, Prdx4 : = 6, and DKO: = 5. < 0.05 versus WT mice. (d) VLDL-TG secretion in mice. Plasma TG levels were measured before and 180min after intravenous tyloxapol injection. TG secretion rate during the 180min experiment was calculated. Data are expressed as the mean SEM ( = 35). < 0.01 versus WT mice. < 0.01 versus Prdx4 mice. (e) Plasma TG levels were measured at 60min intervals after the oral administration of olive oil. Data are expressed as means SEM ( = 34). No difference was observed between the different genotypes.",yes
PMC6667427,Figure_8,oa_package/e2/aa/PMC6667427.tar.gz,"[' 8).', '99mTc-Sestamibi SPECT/CT for localization of parathyroid adenoma (PTA).', 'SPECT/CT (b) performed 1 h after tracer injection localizes this focal uptake to a 16-mm nodule behind the thyroid gland, consistent with a PTALung disordersVentilation and perfusion (V/Q) imaging is routinely used in the workup of patients with suspected pulmonary emboli (PE) [180].']","Fig. 8 Tc-Sestamibi SPECT/CT for localization of parathyroid adenoma (PTA). A 57-year-old woman with laboratory evidence of primary hyperparathyroidism was referred for localization of PTA. Planar scintigraphy ( ) shows an early area of increased focal uptake at the upper pole of the right thyroid lobe (left), with washout of the tracer from adjacent thyroid tissue on late images (right). SPECT/CT ( ) performed 1h after tracer injection localizes this focal uptake to a 16-mm nodule behind the thyroid gland, consistent with a PTA",yes
PMC2918835,Figure_3,oa_package/3e/ff/PMC2918835.tar.gz,"['Frozen section revealed endometriosis, granulomatous inflammation and focal necrosis, with no evidence of malignancy (fig. 3).', 'The 2009 biopsy shows endometriosis and sarcoid commingling.']",Fig. 3 The 2009 biopsy shows endometriosis and sarcoid commingling. The pathologic stains were negative for AFB and fungi (400).,yes
PMC7380008,Figure_3,oa_package/a5/f6/PMC7380008.tar.gz,['Distribution pattern of synuclein in insular subregions.'],"Figure 3 Distribution pattern of synuclein in insular subregions. iLBD shows mild LNs and astroglial synuclein inclusions in layer I of agranular insula ( ), few glial inclusions in dysgranular insula ( ), and sparse dotlike aggregates in granular insula ( ). PD1 agranular insula shows a LBlike inclusion and dotlike aggregates ( ), the dysgranular insula shows bulgy LNs in layer I ( ), and the granular insula shows an intracellular LB inclusion ( ). PD2 shows many LNs inclusions in agranular insula and glial synuclein ( ) and less but bulgy LN in dysgranular ( ) and granular regions ( ). In PDD2 severe astroglial synuclein inclusions are shown in agranular insula ( ) few LBs and LNs in dysgranular insula ( ). The granular insula shows dotlike aggregates and astroglial synuclein . In PDD1 agranular insula, very long LNs and some dotlike aggregates are seen in layer I ( ). Dysgranular insula in PDD1 shows granular cytoplasmic inclusions in neurons and a LB ( ) while the granular insula shows less aggregates and a LB in the infragranular layer ( ). In DLB1, severe synuclein inclusions are seen in agranular insula throughout all layers ( ). Severe astroglial inclusions are seen in the supragranular layers of dysgranular and granular insula ( , ). In DLB2, a cluster of dystrophic LNs and glial inclusions are shown in layer II of the agranular insula ( ). The dysgranular insula contains LNs and dotlike structures ( ) also abundant in the granular insula superficial layers ( ). DLB, dementia with Lewy bodie; iLBD, incidental Lewy body disease; LB, Lewy bodies; LN, Lewy neurites; PD, Parkinson's disease; PDD, Parkinson's disease dementia. Magnification: 630, scale bar 50m.",yes
PMC3108502,Figure_11,oa_package/7c/38/PMC3108502.tar.gz,[],Figure 11 Radiation-associated osteosarcoma of the mandible: (a) Axial contrast-enhanced CT of the mandible (bone window) shows an osteoid matrix (arrow) within the tumor.,yes
PMC9776827,Figure_2,oa_package/40/12/PMC9776827.tar.gz,"['6%) having pCR or near-pCR (MRD) were excluded from the study and 102 patients having enough residual tumors in the surgical pathology specimens were enrolled in the study ().', 'The study recruitment status of 169 patients with breast cancer according to the pathological response degree to NACTx.']","Figure 2 The study recruitment status of 169 patients with breast cancer according to the pathological response degree to NACTx. ( ) IBC on CNB, (100, HE). ( , ) pCR (n = 41, excluded), microscopic appearance of the tumor bed after NACTx. Tumor bed with no residual tumor, characterized by stromal fibrosis and hyalinization, (40 and 100, HE). ( ) IBC on CNB, (100, HE). ( , ) MRD with <10% of tumor remaining (n = 26, excluded). Stromal fibroelastosis and small clusters of residual invasive tumor cells (arrows), (40 and 100, HE). ( ) IBC on CNB, (100, HE). ( , ) Partial response (n = 71, included). Some degree of fibrosis with residual tumor, (40 and 100, HE). ( ) IBC on CNB, (100, HE). ( , ) No response to NACTx (n = 31, included), lumpectomy sample after NACTx, (40 and 100, HE). pCR: pathological complete response; MRD: minimal residual disease; NACTx: neoadjuvant chemotherapy; IBC: invasive breast carcinoma; CNB: core needle biopsy; HE: hematoxylin eosin.",yes
PMC4856964,Figure_2,oa_package/1e/d8/PMC4856964.tar.gz,"['.', 'The transcription factor, Nkx2.']","Figure 2. Pressure overload induced NCX1 upregulation is prevented by Class I HDAC inhibition and Class IIa HDAC5 deletion. ( ) Age and sex-matched wild-type mice were subjected to TAC induced pressure overloaded for 72 h. At the time of surgery mice were injected (intraperitoneal, IP) with the Class I selective HDAC inhibitor, MS275 (10 mg/kg/day) and IP injected daily every 24 h. After 72 h, hearts were harvested, homogenized and lysed. Protein in lysates was quantified and 30 g of each experimental group were then resolved on SDS-PAGE gel and transferred to a membrane for overnight immuno-blotting with anti-NCX1 antibody and GAPDH (loading control). ( ) Age and sex-matched wild-type and HDAC5 mice were subjected to TAC induced pressure overload for 72 h. After 72 h, hearts were harvested and whole heart tissue homogenates were subjected to Western blotting as above with anti-NCX1 antibody and GAPDH (loading control). Western blots shown represent assays that were done in duplicate and repeated three times.",yes
PMC10706067,Figure_4,oa_package/6b/29/PMC10706067.tar.gz,['Planar scintigraphy imaging of a 67-year-old patient who had the extraction of tooth 38 with transient improvement.'],"Figure 4 Planar scintigraphy imaging of a 67-year-old patient who had the extraction of tooth 38 with transient improvement. A few months later, the same pain recurred and was difficult to control with multiple analgesic attempts. Biological results showed no infectious signs. The anterior planar image acquired 6 h after the intravenous injection of anti-granulocyte antibodies showed no radiotracer uptake in the left mandibula (( ), red arrow). Likewise, no radiotracer uptake was seen in the left mandibula on the anterior left profile image acquired 24 h after the radiopharmaceutical injection (( ), red arrow). The blood pool phase (anterior view) of the bone scan showed no left mandibular hyperemia (( ), red arrow). On the other hand, the delayed image of the bone scan (anterior view) showed marked radiotracer uptake (( ), red arrow).",yes
PMC9708566,Figure_2,oa_package/20/b1/PMC9708566.tar.gz,"['2a,b).', '2c,d).', '2a d).', '2e).', '2f,g).', '2f).', '2h).', '2i and Extended Data ', 'Bassoon is associated with tau pathology in human Alzheimer s disease and progressive supranuclear palsy.', 'Source dataBSN enhances tau-seeding activity and toxicityWe overexpressed human P301S tau in human embryonic kidney (HEK) 293T cells with and without human BSN to determine the effect of BSN on the pathological properties of tau.', '2h), we aimed to determine whether this interaction was conformation dependent.', 'pdf"" id=""MOESM7"">Source Data Unprocessed western blots and/or gels.', 'xlsx"" id=""MOESM8"">Source Data Statistical source data.', 'xlsx"" id=""MOESM18"">Source Data Extended Data Statistical source data.']","Fig. 2 Bassoon is associated with tau pathology in human Alzheimers disease and progressive supranuclear palsy. , , Tau-seeding activity of SEC fractions from human AD lysates (from middle frontal gyrus, MFG; ) and PSP lysates (from pons; ), compared to healthy controls from MFG and pons, respectively. , , Total human tau detected by ELISA in SEC fractions from human AD ( ) and PSP ( ) brain lysates compared to healthy controls. , Tau-seeding activity of the AD and PSP SEC fraction 9 (F9) containing HMW tau, before and after hTau IP using HT7 antibody. , , Tau twisted filaments present in the tau-IP product from F9 of AD and PSP SEC fractions, visualized by EM (scale bars, 100nm; ), and width distribution ( ). No filaments were detected in MFG and pons healthy controls. , Co-IP of human tau (HT7) and BSN from SEC F9 in AD, PSP, control MFG and control pons brain lysates. , Colocalization (yellow/white) between BSN (red) and pathological phosphorylated tau species (PHF1, green) in AD and PSP brain sections. The merged panel includes orthogonal images of reconstructed three-dimensional views. Colocalization analysis was performed to determine pixel intensity correlation between PHF1 and BSN. Scale bar, 10m. Data are shown as the means.e.m. Experiments were performed with =3 ( , , and ) and =6 ( ). Significance was determined by unpaired two-tailed Students -test ( ).",yes
PMC4582105,Figure_4,oa_package/fc/fd/PMC4582105.tar.gz,['.'],"Fig. 4. (A) expression (black arrow, upper panels) in early zebrafish embryos defines the non-neural ectoderm field and overlaps with a marker of non-neural ectoderm, (black arrow, lower panels). In Rps19-deficient zebrafish [in which expression has been knocked down with a morpholino oligonucleotide (MO)], this field is expanded (right upper and lower panels). Staining with a probe for goosecoid ( ), necessary for the formation of the dorsoventral axis of the embryo, marks the dorsal side (red arrow). Arrowheads point to the neural field. This is an hybridization image at gastrulation, 80% epiboly ( ). Dorsal is to the right. wt, wild type. (B) Expression of , which has a key role in the development of the CNS, eyes, urogenital tract and kidneys, is altered in Rps19-deficient zebrafish embryos. Arrows and arrowheads point, respectively, to forebrain and eye fields, which are contracted in Rps19-deficient embryos. This is an hybridization image at 16hpf. (C) Schematics showing how expansion of non-neural ectoderm in early zebrafish embryos leads to the contraction of the neural field, especially the area of the forebrain and eye. This research was originally published in ( ). American Society of Hematology.",yes
PMC3657401,Figure_1,oa_package/cd/d6/PMC3657401.tar.gz,"['Multiple lymph nodes measuring up to 19 11 mm were seen in the pericecal region ().', '0-77952633543CT image of the mass located in the cecum.']",Figure 1 CT image of the mass located in the cecum.,yes
PMC4742119,Figure_1,oa_package/6e/eb/PMC4742119.tar.gz,"['The inhibitory effect of PD168393 or Erlotinib on the trans-activation of EGFR by LPSCardiomyocytes were pretreated with LPS (4 g/ml).', '1Effects of erlotinib on survival of mice treated with LPSWild type C57BL/6 mice were pretreated with erlotinib orally 3 days before LPS (20mg/kg) treatment as described in methods A.']","Figure 1 The inhibitory effect of PD168393 or Erlotinib on the trans-activation of EGFR by LPS Cardiomyocytes were pretreated with LPS (4 g/ml). Phospho-EGFR and total EGFR were determined by western blot analysis at 0, 15min, 30min, 60min, and 120min after LPS treatment . Correspondingly gray intensity analysis of the western blot results of five groups . Cardiomyocytes were pretreated with vehicle, PD168393 (10 M), or Erlotinib (20 M) 0.5 hour before LPS (4 g/ml) treatment. Phospho-EGFR and total EGFR were determined by western blot analysis at 0.5 hour after LPS treatment . Correspondingly gray intensity analysis of the western blot results of six groups . Wild type C57BL/6 mice were pretreated with erlotinib orally 3 days before LPS (5mg/kg) treatment. Phospho-EGFR and total EGFR in the myocardium were determined by western blot analysis at 1 hour after LPS treatment . Correspondingly gray intensity analysis of the western blot results of four groups . Each bar represents the mean S.D, * < 0.05, compared with control group; < 0.05, compared with LPS group = 4.",yes
PMC9747669,Figure_1,oa_package/c0/51/PMC9747669.tar.gz,"[' MRI brain axial section showing FLAIR (B) hyper-intensities in the parieto-occipital region, with diffusion restriction (B C) suggestive of cytotoxic edema (infarct), intermixed with vasogenic edema in a patient with eclampsia with symptoms of cortical blindness only.']","Figure 1 MRI brain axial section showing FLAIR (B) hyper-intensities in the parieto-occipital region, with diffusion restriction (B & C) suggestive of cytotoxic edema (infarct), intermixed with vasogenic edema in a patient with eclampsia with symptoms of cortical blindness only. MRI was suggestive of PRES with infarct. FLAIR, fluid-attenuated inversion recovery; PRES, posterior reversible encephalopathy syndrome",yes
PMC6232522,Figure_5,oa_package/e8/55/PMC6232522.tar.gz,['Percent changes in patients with (A C) early onset colon (ages 20 44 years) and rectal cancers (ages 54 years) and (D F) later-onset colon and rectal cancers (defined as age 60 years old).'],Figure 5 Percent changes in patients with early onset colon (ages 2044 years) and rectal cancers (ages 54 years) and later-onset colon and rectal cancers (defined as age 60 years old). Clinicopathological tumors characteristics include cancer staging and tumor histology and grade. Staging based on a merging of AJCC 3rd and 6th editions from the time periods of 19881994 to 19952014. Error bars represent 95% confidence intervals.,yes
PMC8167394,Figure_9,oa_package/1a/4b/PMC8167394.tar.gz,[],Figure 5A. . Most cases are predominantly composed of epithelioid cell with pale eosinophilic cytoplasm.,yes
PMC10257437,Figure_4,oa_package/6e/7e/PMC10257437.tar.gz,"['Post-operative TTE was notable for an improved LVEF of 61% with continued apical akinesis and small aneurysm with possible small associated thrombus ( and Supplementary material online, Video S3).', 'Post-operative parasternal long (A) and apical four-chamber (B) transthoracic echocardiogram images displaying resolution of large left ventricular aneurysm and pericardial effusion.']",Figure 4 Post-operative parasternal long ( ) and apical four-chamber ( ) transthoracic echocardiogram images displaying resolution of large left ventricular aneurysm and pericardial effusion.,yes
PMC5869938,Figure_4,oa_package/6c/99/PMC5869938.tar.gz,"[' 4a) nor in DCX-positive neuroblasts (', ' 4c).', ' 4e).', ' 4b) and DCX-positive neuroblasts (', ' 4d).', ' 4f).', ' 4g).', '', 'Scale bars 25 m\nWe additionally confirmed the presence of transgenic a-syn by Western blot of the dissected hippocampus of NTG, Thy1-E57K, and Thy1-WTS (', ' 4h).', ' 4i).', ' 4) and 8 10-month-old (Rockenstein et al.', ' 4) and may thus elicit similar excitotoxic effects.', ' 4g) are based on direct expression data in neuroblasts together with the correlation to embryonic development.']","Fig.4 Low intrinsic, but high extrinsic transgene expression in adult neuroblasts of Thy1-WTS and Thy1-E57K mice. Colocalization analysis of transgenic a-syn at different stages of adult newborn neuron development. , Sox2-positive stem cells (arrows) were negative for transgenic a-syn in Thy1-WTS and Thy1-E57K. , DCX-positive hippocampal neuroblasts (arrows) were only partly co-labeled with a-syn antibody in Thy1-WTS and Thy1-E57K. , Expression of transgenic a-syn in the dentate gyrus was mainly confined to mature, NeuN-positive granule cells. High expression was noted in the hilus and in the molecular layer. granule cell layer, subgranular zone, hilus. Model of the temporal expression pattern of transgenic a-syn under the control of the PDGF- and Thy1-promoters. Representative western blot and analysis of the levels of a-syn in the hippocampus, showing that highest expression levels of monomeric a-syn (14kDa) are found in Thy1-WTS, whereas dimers (28kDa) and higher molecular weight oligomers (>42kDa) are predominantly present in Thy1-E57K. Scale bars 25m",yes
PMC7780514,Figure_1,oa_package/e3/80/PMC7780514.tar.gz,"['Transoesophageal echocardiography (TEE) revealed no thrombus in the left atrium, and a prominent left atrial septal pouch (LASP) was visualised (figure 1).', 'Mid-oesophageal bicaval view showed the giant membrane of LASP spreading to interatrial septum.', 'However, in our case, the depth of LASP with a giant flap spreading to LA cavity (the white arrows in figure 1) was 30 mm.']","Figure 1 Mid-oesophageal bicaval view showed the giant membrane of LASP spreading to interatrial septum. LA, left atrium; LASP, left atrial septal pouch; RA, right atrium.",yes
PMC11323658,Figure_1,oa_package/ad/ba/PMC11323658.tar.gz,"[' 1.', '\nCONSORT diagram for the cohorts from two different sites in Sweden.', 'WSI = whole slide image\nStatistical analysesWe evaluated the prognostic performance of the patient risk groups (high risk and low risk) assigned by the Stratipath Breast analysis.']","Fig. 1 CONSORT diagram for the cohorts from two different sites in Sweden. ( ) CHIME breast KS Solna cohort with included patient whole slide images from Stockholm, Sweden. ( ) SCAN-B cohort with included patient whole slide images from Lund, Sweden. NKBC=National quality registry for breast cancer. WSI=whole slide image",yes
PMC4161480,Figure_7,oa_package/ef/0a/PMC4161480.tar.gz,"['Although some variability in the expression levels was noted, significantly decreased mRNA expression was also noted in epithelial cells devoid of Tlr2, Tlr5, or Nod2 (A B).', 'Of note, intestinal colonization (C), enterocyte invasion (', '7E G) and dissemination to systemic organs (C D) was observed also in the absence of the most potently stimulated innate immune receptor, Tlr4.', 'g007Epithelial innate immune stimulation during neonatal Salmonella infection.']",10.1371/journal.ppat.1004385.g007,yes
PMC5618150,Figure_6,oa_package/d3/4a/PMC5618150.tar.gz,"['ThT assay (a) showed the varied\naggregation propensity in the order: Ac-NNFGAILVV Ac-NNFGAILTT\n \nAc-NNFGAILSS Ac-NNFGAILAA, which correlated with the twisted/untwisted\nmorphologies.', 'As proposed above and depicted in b, stronger -sheet registry led to\nuntwisted ribbons of Ac-NNFGAILVV (c) and Ac-NNFGAILTT (a), whereas twisted fibrils were formed in\nAc-NNFGAILSS (a) and Ac-NNFGAILAA (d).', 'In particular, the aggregation propensity as determined\nby the ThT assay (a) could correlate with the residue hydrophobicity, in which\nvaline possessed the highest hydrophobicity while serine and alanine\nwere more hydrophilic.', 'Effect of amino acid side chains on the peptide aggregation\nand\nfibril morphology.', 'pdf"">s S4, S6, and 6a, substitution of serine with threonine enhanced\nthe aggregation propensity of amyloid peptides (To ensure completion of the replication cycle in the Spinach2 TBEV replication system, firefly luciferase activity translated from progeny positive sense replicon RNAs, was measured in both PS (C) and A549 (', 'Replicon-derived luciferase activity, when normalized to the levels of cotransfected renilla luciferase, reflected the levels of +ve sense RNA, with an early but short-lived peak for Hypr and a slower, more prolonged signal for Vs (C and D).', 'We validated the TBEV replicons by assessing their ability to recapitulate virus replication kinetics ().', 'We found that Hypr replicates to high levels at early time points, peaking at 24 hpt and declining thereafter ( and 4).', 'This contrasted with the Vs replicon, which produces lower and more sustained levels of viral RNA synthesis that persist for longer ( and 4).']","FIG 2 Characterization of the TBEV Spinach2 replicon in mammalian cells. (A) qRT-PCR analysis in TBEV replicon-transfected PS cells. Data are the mean SEM of two independent experiments. (B) Culture media collected from TBEV-infected PS cells (MOI 0.1) at the indicated times postinfection (pi) were assessed for virus infectivity via plaque assay. Data are the mean SEM of three independent experiments. (C and D) Luciferase assays of PS or A549 cells cotransfected with WT TBEV replicon (Hypr or Vs) and pTK-Ren (renilla) performed at the indicated time points posttransfection. Firefly values were normalized to renilla. Bar heights represent the mean SEM of three biological replicates. Assays were performed in triplicate. , < 0.01; * , < 0.001, from WT Hypr determined using a two-tailed Student's test with Welchs correction.",yes
PMC4090635,Figure_1,oa_package/2a/b2/PMC4090635.tar.gz,"['Pelvic ultrasonography (US), CDUS and CT images showed multiple pelvic varices including tortuous pelvic veins, dilated arcuate veins in the myometrium communicated with pelvic varicose veins ().', '.']","Figure 1. A 27-year-old woman with NCS and pelvic congestion syndrome A) CDUS and (B, C) sagittal CT images show reduction of the angle between AA and SMA (14.5) (arrows). D) Pelvic CDUS shows pelvic varicose veins (arrows).",yes
PMC8546822,Figure_3,oa_package/eb/b7/PMC8546822.tar.gz,['\nImaging and pathology findings at the 2nd recurrence.'],"Figure 3 A: Ultrasonography shows a series of masses of up to 27 mm located on the right side of the previous surgical wound; B: Enhanced breast magnetic resonance imaging showed multiple masses up to 25 mm in the right inner-upper area (white arrow). The masses were cystic with thick walls, similar to the previous tumor, and were diagnosed as recurrent adenomyoepithelioma. Two 7-mm nodules were also found within the pectoralis major muscle (arrowhead); C: The tumor was a cystic lesion, and the cystic wall had nodules or irregular thickening. The cyst contained mucus ( 3 magnification); D: Biphasic proliferation of both inner epithelium with squamous metaplasia and outer spindle-shaped myoepithelium was seen ( 100 magnification). Both cell types showed prominent nuclear atypia and high mitotic counts that were especially prominent in the epithelial component. The histological findings were the same as those of the previous surgery; E: The tumor cells invaded the extramammary adipose tissue.",yes
PMC7410356,Figure_2,oa_package/12/72/PMC7410356.tar.gz,"[' 2) defined by scattered or diffuse hyaline membranes, associated in some areas with alveolar oedema, an alveolar eosinophil exudate and a few vacuolated macrophages; and a more organized stage (', 'Microscopic findings in the lungs.', 'b Hyaline membranes and alveolar vacuolated exudates (arrow)Microscopic findings in the lungs.']","Fig. 2 Microscopic findings in the lungs. (HES, magnification 20). Diffuse alveolar damage in the (exudative) stage. Hyaline membranes (arrow). Hyaline membranes and alveolar vacuolated exudates (arrow)",yes
PMC6807377,Figure_5,oa_package/fa/be/PMC6807377.tar.gz,"['The tumor replaced the right pedicle, lamina, and spinous process at C7, with extraosseous extension into the soft tissues around the right brachial plexus and into the superior sulcus of the right hemithorax ( 5).', ' 5Pretreatment coronal short-TI inversion recovery magnetic resonance image (MRI) (A) and contrast-enhanced coronal T1W MRI (B) showing large predominantly solid mass with scattered small internal cysts arising from the collapsed C7 vertebral body and right pedicle and growing extracompartmentally to surround the right brachial plexus.']",Figure5 Pretreatment coronal short-TI inversion recovery magnetic resonance image (MRI) ( ) and contrast-enhanced coronal T1W MRI ( ) showing large predominantly solid mass with scattered small internal cysts arising from the collapsed C7 vertebral body and right pedicle and growing extracompartmentally to surround the right brachial plexus.,yes
PMC4246630,Figure_4,oa_package/21/86/PMC4246630.tar.gz,"['A computed tomography (CT) scan of the abdomen revealed cervical cancer following chemoradiotherapy and lower intestinal obstruction ().', 'Abdominal computed tomography scan revealing lower intestinal obstruction.']",Figure 4 Abdominal computed tomography scan revealing lower intestinal obstruction.,yes
PMC11588598,Figure_3,oa_package/68/e1/PMC11588598.tar.gz,"['Patient underwent left pterional craniotomy with anterior temporal approach through trans-Sylvian, transcavernous corridor with mobilization of the third nerve and microsurgical clipping of left superior cerebellar artery aneurysm (\n\n) with encephalo-duro-arterio-myo-synangiosis (EDAMS) on the same side (left).', '\n(\nA\n) Showing intraoperative picture of left superior cerebellar artery (SCA) aneurysm before clipping and (\nB\n) showing picture of left SCA after clipping (green arrow shows aneurysm and yellow arrow shows 3rd nerve).']",Fig. 3 ( ) Showing intraoperative picture of left superior cerebellar artery (SCA) aneurysm before clipping and ( ) showing picture of left SCA after clipping (green arrow shows aneurysm and yellow arrow shows 3rd nerve).,yes
PMC5597866,Figure_2,oa_package/27/be/PMC5597866.tar.gz,"[':Gastroscopy showing the impacted stone (a), stone retrieval (b) and a patent lumen post procedure (c).']","Figure 2: Gastroscopy showing the impacted stone ( ), stone retrieval ( ) and a patent lumen post procedure ( ).",yes
PMC10162885,Figure_2,oa_package/d5/d5/PMC10162885.tar.gz,['003*Response to the treatment in standard treatment control group: (A) Baseline and (B) after 12 weeks of treatmentResponse to the treatment in the add-on apremilast group: (A) Baseline and (B) after 12 weeks of treatmentForty-five adverse events in 16 patients were observed in the add-on apremilast group.'],Figure 2 Response to the treatment in standard treatment control group: (A) Baseline and (B) after 12 weeks of treatment,yes
PMC10442706,Figure_3,oa_package/0c/ef/PMC10442706.tar.gz,"['As the research on ketamine s involvement in enhancing synaptogenesis has progressed, a set of diverse, but not mutually exclusive mechanisms have been identified (), that may act synergistically to induce a sustained strengthening of excitatory synapses (e.', 'A highly simplified illustration of some of ketamine s hypothesized antidepressant mechanisms of action at an inter-and intraneuronal level.']","Figure 3 A highly simplified illustration of some of ketamines hypothesized antidepressant mechanisms of action at an inter-and intraneuronal level. Ketamine antagonize NMDARs (green) of GABAergic interneurons (light blue), causing disinhibition of pyramidal neurons (beige, top), resulting in a glutamate (Glu) surge. Activation of postsynaptic AMPAR (dark blue) initiate intracellular cascades which in turn increase synaptic BDNF levels and TrkB activation. Ketamine also antagonizes post-synaptic NMDARs, deactivating eEF2K, leading to desuppression of BDNF and mTOR. Increased mTOR phosphorylation leads to increases in levels of PSD-95 and AMPAR subunits. GSK-3 is inhibited by ketamine, resulting in increased mTOR and PSD-95 activity. In the astrocyte (yellow) ketamine downregulates activity of the Kir4.1 channel, causing less influx of potassium (K+), depolarization of the cell, and by consequence, reduction of EAAT activity and reduced retrieval of glutamate from the synapse. Ketamine also increase cholesterol (Chol) density in the astroglial membrane, which is hypothesized to cause a flux of cholesterol to nearby neurons. Astrocytes are also shown to produce and release more kynurenic acid (KynA) after ketamine treatment, and could also have impaired BDNF reuptake ability as ketamine stabilizes astrocytic fusion pores in a narrow configuration. Ketamine also affects microglia (red) and inflammation, attenuating hyperactivity of proinflammatory cytokines, and reducing levels of quinolinic acid (QUIN).",yes
PMC6302239,Figure_4,oa_package/09/0f/PMC6302239.tar.gz,"[':Laparoscopic view of the urachal cyst, viewing the umbilical portion of the urachus.']","Figure 4: Laparoscopic view of the urachal cyst, viewing the umbilical portion of the urachus.",yes
PMC11557655,Figure_5,oa_package/d2/1d/PMC11557655.tar.gz,"[' 5 and 6.', 'Bar chart showing the differences in opinions amongst the respondents about their willingness for AI to be used to interpret imaging for other diseases, such as cancer, cardiac or brain diseases.', 'There did not appear to be a large difference in those agreeing or disagreeing for AI usage for the different diseasesBar chart showing the differences in opinions amongst the respondents about the perception of AI accuracy for detecting other diseases such as cancer, cardiac or brain diseases.']","Fig. 5 Bar chart showing the differences in opinions amongst the respondents about their willingness for AI to be used to interpret imaging for other diseases, such as cancer, cardiac or brain diseases. There did not appear to be a large difference in those agreeing or disagreeing for AI usage for the different diseases",yes
PMC5348315,Figure_6,oa_package/15/6c/PMC5348315.tar.gz,"['Effect of glycine treatment on macrophage infiltrationStandard control group(C), high fat and high sugar group (H), high fat and high sugar + glycine group (H+G), glycine group (G).']","Figure 6 Effect of glycine treatment on macrophage infiltration Standard control group(C), high fat and high sugar group (H), high fat and high sugar + glycine group (H+G), glycine group (G). *indicates a statistically significant difference ( < 0.05).Data represents mean standard error ( = 8).",yes
PMC8825615,Figure_3,oa_package/7c/6a/PMC8825615.tar.gz,"[' 3).', 'The brain regions contributing to SZ prediction in elastic net logistic regression.', 'Abbreviations: SZ, schizophrenia; ALFF, amplitude of low-frequency fluctuations; GM, gray matter; L, left; R, rightCorrelations with PANSSThe 8 common regions involved in both ALFF and GM models were selected to examine the relationship with PANSS scores.']","Fig. 3 The brain regions contributing to SZ prediction in elastic net logistic regression. Twenty-seven regions were selected in the ALFF analysis, and 26 regions were selected in the GM analysis. There were 8 common regions in both two analysis. Abbreviations: SZ, schizophrenia; ALFF, amplitude of low-frequency fluctuations; GM, gray matter; L, left; R, right",yes
PMC11338256,Figure_1,oa_package/37/71/PMC11338256.tar.gz,['.'],Fig. 1. (A) Multiple alopecic patches of the scalp associated with diffuse scaling and thin and brittle hair. (B) Circumferential hairline recession associated with brown lamellar scales on the scalp. (C) Hairline recession of the posterior scalp associated with brown lamellar scales. (D) Hair line recession of the frontal scalp associated with yellow scales.,yes
PMC10619506,Figure_3,oa_package/fc/f8/PMC10619506.tar.gz,"['The majority of LacZ+ cells were localized in LTL+ PTECs in the cortex and OSOM (, A and B).', 'Less than 1% of LacZ+ cells were Kim1+ PTECs 24 hours after injury, increasing to 17% in the OSOM and 21% in the cortex 72 hours after injury (, C and D).', 'RAR signaling is extensively activated in Kim1 PTECs after Rhabdo-AKI.']","Figure 3 RAR signaling is extensively activated in Kim1 PTECs after Rhabdo-AKI. RARE-LacZ reporter mice were used to evaluate the cellular localization of RAR signaling after Rhabdo-AKI. ( and ) Percentage of LacZ cells in LTL and Kim1 PTECs ( ) and in Kim1 PTECs ( ) at the different time points after injury in the cortex and OSOM: 2 mice before injury (day 0) and 4 at days 1 and 3, 3 at day 7, and 5 at day 14 after injury. ( and ) Images showing LacZ, LTL, and Kim1 staining in the cortex and OSOM. ( ) Low-power images of the kidneys; regions of the kidney are demarcated by dotted lines, as indicated. Scale bars: 500 mM. ( ) High-power images of the OSOM. Scale bars: 100 mM.",yes
PMC7588142,Figure_9,oa_package/ec/68/PMC7588142.tar.gz,[],"Figure S5. . Enrichment of Rel68 in promoter region of or assessed by ChIP-PCR analysis. The enrichment signals (IP/Input) of promoter region were normalized with those of ORF region. *, P < 0.05 by Students test; error bars, SEM; = 3 independent experiments (Locus amplified by primers in qPCR experiments is marked with black bars in upper panels).",yes
PMC5719970,Figure_1,oa_package/c9/4f/PMC5719970.tar.gz,"['2/30 column (GE Healthcare) and revealed a near complete conversion from monomers to oligomers (-1B).', ', 2015) expressing the markers nestin, vimentin, S100 , and GFAP (-1A) were exposed to, 0.', '5 m Cy3-labeled -SYN oligomers (-1B) for 24 h (see schematic outline in ', 'Live-cell imaging starting instantly after exposure visualized uptake of -SYN oligomers (starred in the figures) in the astrocytes (-2A).', 'After engulfment (-2A, 11 h 25 min to 11 h 55 min), the astrocytes contracted and temporarily rounded up as -SYN was transported to the region around the nuclei (', 'Moreover, we confirmed the intracellular location of the engulfed -SYN-Cy3 oligomers in the astrocytes by actin labeling and confocal 3D imaging (B).', 'To investigate whether the -SYN inclusions were degraded by the human astrocytes, the cells were thoroughly washed after the 24 h -SYN-Cy3 oligomer exposure and cultured for an additional 3 or 6 d in -SYN-free medium before fixation (see schematic outline in A).', 'Interestingly, we found that the ingested -SYN oligomers remained in the cells throughout the experiment (-2B).', 'To verify this result, we performed Western blot analysis of total whole-cell lysates and pellet fractions with antibodies directed to -SYN (-2C).', 'As expected, SDS treatment of the -SYN oligomers resulted in dissociation into a wide range of high- and low-molecular-weight -SYN species (-2C; N sstr m et al.', 'Interestingly, our data show that there was a reduction in high-molecular-weight SYN species in the lysates over time, but the high-molecular-weight SYN species in the pellet fraction remained rather intact at all three time points, demonstrating that the -SYN oligomers were not effectively degraded by the cells over the 6 d time period (-2C).', 'In contrast to the oligomers, monomeric -SYN was degraded rapidly by the human astrocytes (-3, A,B).', '.', 'Human ESC-derived astrocytes (see -1A) were exposed to 0.', '5 m Cy3-labeled -SYN oligomers (see -1B) for 24 h, washed thoroughly, and cultured for an additional 0, 3, or 6 d in -SYN-free medium before fixation (A).', 'The intracellular location of the -SYN-Cy3 after ingestion (see -2A) was confirmed with confocal imaging (B).', 'Quantification of the -SYN-Cy3 signal at days 0, 3, and 6 (C and -2B) reveled the total area of the -SYN deposits per astrocyte in square millimeters (D), the number of -SYN deposits per astrocyte (E), the mean area of the deposits in square millimeters (F), and the mean intensity of the intracellular -SYN-Cy3 per field (G).', 'Western blot analysis confirmed the presence of high-molecular-weight -SYN species in the astrocytes at all three time points (-2C), demonstrating that only minimal degradation of the ingested oligomeric -SYN occurred, whereas the monomeric -SYN was completely degraded during the 6 d time period (see ', '10.', 'f1-1-1Immunocytochemical characterization of the human ESC-derived astrocytes demonstrated positive staining using specific antibodies to vimentin, nestin, S100 and GFAP (A).', 'tif"" content-type=""local-data"">-1, TIF file10.', 'f1-2-2Time lapse experiment demonstrated uptake of Cy3- -SYN oligomers (yellow star) in astrocytes.', 'tif"" content-type=""local-data"">-2, TIF file10.', 'f1-3-3Monomeric Cy3- -SYN was effectively degraded by the human ESC-derived astrocytes.', 'tif"" content-type=""local-data"">-3, TIF fileQuantifications of the Cy3 signal at the different time points after oligomer exposure (C) demonstrated that the total area of the inclusions per astrocyte (', '1D) and the number of deposits per astrocyte (E) were stable over time and did not change significantly from days 0 to 6, indicating minimal degradation of the ingested aggregated -SYN during the 6 d time period.', 'However, the mean area per inclusion (F) and the mean intensity per field (']","Figure 1. Intracellular -SYN is stored rather than degraded. Human ESC-derived astrocytes (see ) were exposed to 0.5 Cy3-labeled -SYN oligomers (see ) for 24 h, washed thoroughly, and cultured for an additional 0, 3, or 6 d in -SYN-free medium before fixation ( ). The intracellular location of the -SYN-Cy3 after ingestion (see ) was confirmed with confocal imaging ( ). Quantification of the -SYN-Cy3 signal at days 0, 3, and 6 ( and ) reveled the total area of the -SYN deposits per astrocyte in square millimeters ( ), the number of -SYN deposits per astrocyte ( ), the mean area of the deposits in square millimeters ( ), and the mean intensity of the intracellular -SYN-Cy3 per field ( ). Western blot analysis confirmed the presence of high-molecular-weight -SYN species in the astrocytes at all three time points ( ), demonstrating that only minimal degradation of the ingested oligomeric -SYN occurred, whereas the monomeric -SYN was completely degraded during the 6 d time period (see , ). Scale bars: , 10 m; , 20 m. Data are presented as mean SD from four independent experiments and the levels of significance were set to * < 0.05, ** < 0.01, and *** < 0.001 ( ).",yes
PMC8499532,Figure_4,oa_package/ae/a2/PMC8499532.tar.gz,"[' 4); 20% tumor bleeding and necrosis; invasion of tumor tissues to the renal capsule, (renal sinus) adipose tissues, renal pelvis, and ureter; and absence of tumor infiltration to the renal hilum and retrocapsular lymph nodes (in turn, 0/8, 0/2).', 'Postoperative pathology: left nephroblastoma (hybrid)LiteratureThe PubMed database was searched from 2010 to 2020 for the terms Wilms tumor and Duplex and Wilms tumor and Rupture.']","Fig. 4 Postoperative pathological image (Zeiss Axio Scope A1,Mingmei Digital Imaging System MD50-B, HE staining, 1010): left nephroblastoma (mixed type). Postoperative pathology: left nephroblastoma (hybrid)",yes
PMC6927550,Figure_2,oa_package/c5/78/PMC6927550.tar.gz,"['These lesions included normal with no hyperplasia/dysplasia (1/12), simple epithelial hyperplasia with no dysplasia (2/12), indefinite for dysplasia (reactive epithelium and/or atypical hyperplasia) (7/12), low grade adenoma (1/12) or high grade adenoma (1/12) (G, H) compared to lack of similar range of proliferative and dysplastic lesions in the uninfected WT mice (E, F and Table 2).', 'The gastric antrum/pylorus of uninfected Muc5ac / mice did not express Muc5ac as expected, but showed moderate to strong expression of Muc1, Tff2 and Tff1 within the hyperplastic and neoplastic glandular and surface epithelium ().', 'Other than Muc2, which is not typically expressed in the normal stomach, all other mucins and trefoil factors displayed their typical immunostaining pattern in the antrum/pylorus of the WT murine stomach (), with the expression patterns of Muc5ac and Muc1 more or less similar in distribution.', 'Bars: A, C- 400 M, B-160 M, D-80 M:Immunohistochemical analysis of the antrum/pylorus for various mucins and trefoil factors:Panels shows staining pattern of various markers in normal uninfected WT mice and polypoid adenoma in the antro-pyloric region of uninfected Muc5ac / mice at 39 weeks of age.']","Figure 2: Immunohistochemical analysis of the antrum/pylorus for various mucins and trefoil factors: Panels shows staining pattern of various markers in normal uninfected WT mice and polypoid adenoma in the antro-pyloric region of uninfected mice at 39 weeks of age. Note lack of epithelial Muc5ac and Muc2 staining in antrum/pylorus and increased staining intensity for Muc1, TFF2 and TFF2 within the adenoma/hyperplastic antrum/pylorus. Bars: 160M: All WT images. 400M: All images. Arrows indicate micro adenomas with divergent differentiation within the large adenoma in the pylorus.",yes
PMC3621222,Figure_1,oa_package/82/82/PMC3621222.tar.gz,"[""The Snoopy's sign was present []."", 'Snoopy sign.']",Figure 1 Snoopy sign. Lung tissue between aorta and pulmonary artery,yes
PMC8971723,Figure_1,oa_package/19/ee/PMC8971723.tar.gz,[],"Figure1 The CT, MRI manifestation of PLNTY. Dense calcifications were often demonstrated in CT images . The MRI scans often appeared as iso- or low signal in T1W1 and also can be slight hyperintense because of the composition of calcium salts . The increased or mixed-signal were seen in T2W2 , and the cystic components were also seen in . No enhancements were shown in post-gadolinium T1-weighted MR images .",yes
PMC10473882,Figure_4,oa_package/09/96/PMC10473882.tar.gz,"['Examination with the Goldmann lens for gonioscopy revealed dysgenesis of Schwalbe s line associated with peripheral anterior synechiae in the RE that produced traction of the iris with a marked displacement that hid the pupil, giving the characteristic appearance of pseudoacorea () and the LE it only showed a tenuous posterior embryotoxon.', ':Gonioscopy of the RE: Schwalbe s line dysgenesis associated with peripheral anterior synechiae that produced traction of the iris with a marked displacement that hid the pupil giving the characteristic appearance of pseudoacorea was evidenced.']",Figure 4: Gonioscopy of the RE: Schwalbes line dysgenesis associated with peripheral anterior synechiae that produced traction of the iris with a marked displacement that hid the pupil giving the characteristic appearance of pseudoacorea was evidenced. Source: A Own creation taken from Alza A and Galletto E (2022) Retroiridian pupilloplasty. Clinical and Experimental Ophthalmology 15(1): e40i-e47i.- B Own creation.,yes
PMC5588676,Figure_5,oa_package/e7/b9/PMC5588676.tar.gz,"[' 5b) compared to wild-type littermates (', ' 5a).', 'pSyk is increased in cortical neurons of Tg Tau P301S mice compared to wild-type littermates.', 'The scale bars represent 100 m\nWe also analyzed the temporal changes of pSyk and tau levels in hippocampi and cortices of Tg Tau P301S mice between the age of 8 and 56 weeks (Figs.']","Fig. 5 pSyk is increased in cortical neurons of Tg Tau P301S mice compared to wild-type littermates. Representative confocal image depicting 56week-old male Tg Tau P301S and wild-type mice stained with antibodies against pTau (S202, purple), pSyk (Y525/526, ). Wild-type mice did not exhibit any tau phosphorylation nor Syk activation in their cortices. Tau-overexpressing Tg Tau P301S mice exhibited a prominent tau phosphorylation at S202 that colocalized with Syk activation in cortical neurons. The scale bars represent 100m",yes
PMC11347286,Figure_2,oa_package/15/3d/PMC11347286.tar.gz,"[', 2023) (A).', 'With a low density of actin crosslinking proteins, smaller force exerting on FAs makes FAs less stable, leading to an increase in the speed of cell migration with a higher FA turnover rate (B).']","FIGURE 2 Contribution of actin crosslinking proteins to ECM rigidity sensing. Traction stress images of -actinin KD and non-target (control) C2C12 myoblast cells on fibronectin-coated 2.3kPa and 16.3kPa polyacrylamide gel substrates. Non-target cells on stiffer substrates exerted larger traction stress than on softer substrates. The rigidity-dependent increase in traction stress was attenuated in -actinin KD cells. Yellow lines indicate outlines of cells. The heatmap scale of traction stress is common between non-target and -actinin KD cells. Scale bars, 20m. Schematic diagram of the possible roles of actin crosslinkers in cell migration. Cells with a high density of actin crosslinking proteins transmit contractile force more efficiently to FAs, which stabilizes FAs, attenuates FA turnover, and, thereby, lowers the cell migration speed.",yes
PMC10363678,Figure_4,oa_package/14/dd/PMC10363678.tar.gz,"['Emergency cranial computed tomography (CT) confirmed the left remaining occipital lobe mass ().', 'Axial cranial CT image.']","Figure 4 Axial cranial CT image. Confirmed that the left remaining occipital lobe mass (measuring 6.7 4.2 cm), with increased surrounding hemorrhage, and herniation formation of the left temporal lobe hook gyrus and parahippocampal gyrus.",yes
PMC8177680,Figure_6,oa_package/11/78/PMC8177680.tar.gz,"['As depicted in , such lesions are caused by upward and downward movements of the brain, resulting from a linear force applied to the head(8).', 'Abducens nerve trauma.']","Figure 6 Abducens nerve trauma. A 50-year-old male with convergent strabismus after a motor vehicle accident. : Axial contrast-enhanced T1-weighted spoiled gradient-recalled MRI sequence showing enhancement of the abducens nerve. : Axial T2-weighted MRI scan showing a hyperintense signal, consistent with acute denervation, in the left lateral rectus muscle.",yes
PMC7354736,Figure_2,oa_package/a9/1c/PMC7354736.tar.gz,"['Indeed, neck ultrasound (A) and 99TcSestaMIBI parathyroid scintiscan showed a large mass in the anterior-right neck region, consistent with a hyperfunctioning parathyroid adenoma (', '(A and B) Hyperfunctioning parathyroid adenoma, described by different imaging techniques.', '\nTable 1Main biochemical parameters at baseline and after surgery.', 'At this point, given the diagnosis of pHPT, selective mini-invasive parathyroidectomy was performed (C).']","Figure 2 (A and B) Hyperfunctioning parathyroid adenoma, described by different imaging techniques. (A) Sonographic image in the longitudinal plane demonstrated a 91520 mm, hypoecoic lesion, lodged at the right-inferior thyroid bed and suggestive of parathyroid adenoma or thyroid residual tissue; (B) TC-SestaMIBI scintiscan described a focal hypercaptation at right-inferior thyroid bed, confirming the nature of the lesion. (C) Gross photograph of the right-inferior parathyroid adenoma, surgically removed. The mass measured 22176 mm. Notice the area of parathyroid hypotrophic tissue at periphery. When sliced, it appeared as a solid and whitish mass with brown spots.",yes
PMC6817668,Figure_3,oa_package/b0/6e/PMC6817668.tar.gz,"['Global pseudotemporal analysis of this dataset identified eight temporally distinct gene sets (GS1 GS8) with distinct ontologies (s 3A and 3B, and Table S12).', 'GS6 GS8 gene-associated phenotypes included defects in ureteric bud, renal system, and podocyte foot processes accompanied with GO Terms for regulation of development, cell adhesion, and cell movement ( 3B and Table S12).', ' 3Trajectory Analysis of Podocyte Lineage Cells Identifies Distinct Transient Gene Expression Signatures(A) Unidirectional trajectory of undifferentiated NPCs and podocyte lineage cells (see Transparent Methods) identified in 2G.', ', 1994), and NTN4 (GS8; a Netrin family member) (C) (Yin et al.']","Figure2 Single-Nucleus RNA Sequencing of the Developing Human Kidney and Pseudotemporal Ordering Resolves Major Cell Types and Informs on Podocyte Developmental Programs (A) An overview of the snDrop-seq pipeline on fetal kidney samples ( ) including dissociated kidney cortical cells (14 and 16weeks) and sieved glomeruli (13 and 15weeks). (B) Combined expression data (four individuals over five experiments) visualized by UMAP dimensional reduction showing 18 distinct cellular populations encompassing the nephrogenic, interstitial, endothelial, and immune cell types. Cell type annotations and associated select marker genes are indicated for each cluster. (C) Violin plots showing expression values for select broad or cell-type specific marker genes. For each cluster, the number of datasets (SN), average number of transcripts (UMI), and average number of genes detected are indicated. (D) SWNE visualization of nephrogenic lineage cell clusters and associated non-negative factorization (NMF) identified gene signatures indicate cluster relationships. (E) Heatmap of top genes associated with each NMF state. (F) Trajectory analysis using Monocle of nephrogenic lineages showing developmental progression into distinct RC (proximal) and tubule (medial/distal) lineages. Corresponding heatmap indicating discrete gene expression intensity from the progenitor state (gray center line value) maturing to either RC (redtip value) or medial/distal (blue tip value) branches. (G) Trajectory analysis of the developing RC branch into either parietal or visceral (podocyte) epithelial cell types. Heatmap showing expression values of select marker genes progressing from differentiating NPCs (center, gray) to either parietal (red) or podocyte (blue) cell types. See also .",yes
PMC8783999,Figure_5,oa_package/de/bd/PMC8783999.tar.gz,"['5, 6)A total of 28 cases of syndromic association were identified, representing approximately 16% of the patients presenting a congenital heart disease and approximately 27% of the patients (28 of 105) positive for a non-cardiovascular CT finding.', '"" align=""char"">1Williams Beuren syndrome.', 'Kartagener syndrome.']","Fig. 5 Williams Beuren syndrome. Supravalvular aortic stenosis associated with a tight stenosis of the root of the right pulmonary artery [arrows ( , )]. Congenital lobar emphysema of the superior pulmonary left lobe ( , ).",yes
PMC8429736,Figure_7,oa_package/e5/e0/PMC8429736.tar.gz,"[' 7A) and Flutemetamol55 (data not shown) showed complete displacement of both radioligands.', 'Reduced binding of CHDI-626 to human AD brain.', 'A competition assay format was applied with 4 as a radioligand (replacing the initially used 5) to assess competition potency of test compounds that may reflect specificity against beta amyloid/tau aggregates in AD patient brain homogenates (i.', ' 7A).', ' 7A) with a maximum inhibition of 5% at 1 M but retaining high affinity for HTT aggregates in RBA and ARG (Table 1; Supplementary ', ' 7B, [3H]CHDI-626 exhibited significantly higher binding to sections from HD donors compared to CTRL tissue (2.', ' 7C.', ' 7A C).', ' 7A) and in human post-mortem brain sections from HD, AD and CTRL subjects using in situ autoradiography (', ' 7B, C) and HR ARG (']","Figure 7 Reduced binding of CHDI-626 to human AD brain. ( ) ADbh RBA. Competition format against to determine inhibition of compounds at 1M and 0.1M, applied as counter assay; the dotted line at 30% inhibition indicates the threshold above which a compound is flagged as a potential beta amyloid binder. ( ) Comparative ARG studies investigating ligand binding to human HD, AD and CTRL brain sections. Total (TB) and non-specific (NSB) of [ H]CHDI-180 and [ H]CHDI-626 to human brain sections in ARG. Black circles indicate binding to individual adult HD brains, the white triangle depicts binding to one juvenile HD case. Data shown as Medianinterquartile range. Statistical analysis by MannWhitney test, two-tailed, 95% confidence level. LLoQ: lower limit of quantification ( ) Representative images of [ H]CHDI-626 total binding (TB) and non-specific binding (NSB) in human HD, AD and CTRL brain sections. Toluidine blue staining was used to discriminate white matter (dark gray) and gray matter (light gray) in the same section used for autoradiography.",yes
PMC11587679,Figure_3,oa_package/ec/8e/PMC11587679.tar.gz,"[' 3A-F).', ' 3B, C, E).', ' 3B).', ' 3C).', ' 3E).', ' 3A and D) (', '\nOXPHOS levels in hypothalamic mitochondria of 3xTg male and female mice compared to controls.', 'Additionally, there were no significant variations in the levels of protein subunits for complexes I and IV across all age groups, regardless of genotype or sex (D)\n\nCytochrome c oxidase (COX) activity in 3xTg vs.']","Fig. 3 OXPHOS levels in hypothalamic mitochondria of 3xTg male and female mice compared to controls. Western blot images show representative bands for NADH dehydrogenase beta subcomplex subunit 8 of Complex I (NDUFB8; ), succinate dehydrogenase subunit B of complex II (SDHB; ), cytochrome b-c1 complex subunit 2 of complex III (UQCRC2; ), cytochrome c oxidase subunit 1 of complex IV (MTCO1; ), and ATP synthase subunit alpha of complex V (ATP5A; ). Quantification of these proteins, normalized to total protein ( ) for 3xTg female and male mice and their respective controls at 2, 6, and 13 months of age. Asterisks indicate statistical significance compared to corresponding control groups. Results are presented as meanSD of =4 per group, with significance levels denoted as * 0.05, ** 0.01, and *** 0.001, analyzed using functional three-way ANOVA. In female 3xTg mice, complex II expression was significantly reduced compared to males, while no sex-based differences were observed in control mice across all age groups ( ). Complex III levels were higher in female 3xTg mice at 2 and 6 months, whereas control mice showed an increase only at 13 months ( ). No significant differences in complex V expression were noted in 3xTg mice; however, at 2 months, control mice exhibited higher levels in females, with no notable differences in other age groups ( ). Additionally, there were no significant variations in the levels of protein subunits for complexes I and IV across all age groups, regardless of genotype or sex ( )",yes
PMC3297633,Figure_8,oa_package/b9/46/PMC3297633.tar.gz,"['ulcerans infection lead to severe structural damage during the first 70 days of infection, ultimately resulting in extensive areas of cell death, necrotic alterations, and bacterial colonization (D and I), as recently reported in detail 10 immunoglobulin G4 + plasma cells/high-power field (e). However, VerhoeffVan Gieson Stain did not reveal prominent obliterative phlebitis (f) Case 2 Nodular fasciitis (g-l): Photograph shows gross excisional specimen (g) which on histopathology reveals hypercellular stroma with fascicular and storiform arrangement with skeletal muscle invasion (h), few mitotic figures (i) and positivity for S100 (j), low Ki-67 proliferation index (k) and vimentin positivity (l)",yes
PMC3669697,Figure_6,oa_package/b1/9d/PMC3669697.tar.gz,"['The patient made uneventful recovery and post operative cervical MRI showed resolution of the arachnoid cyst ().', 'Postoperative T2-weighted magnetic resonance sagittal images of the cervical spine showing near to complete resolution of the cyst.']",Fig. 6 Postoperative T2-weighted magnetic resonance sagittal images of the cervical spine showing near to complete resolution of the cyst.,yes
PMC6071199,Figure_1,oa_package/15/b5/PMC6071199.tar.gz,"['5 mm, DV: -2 mm; A,B) and cemented to the skull.', '.', 'Electrode placement predominantly targeted the ACC portion of the PFC (Schweimer and Hauber, 2005; A,B).']",,yes
PMC8359155,Figure_3,oa_package/09/60/PMC8359155.tar.gz,[],"FIGURE 3 Activation of the small GTPase Rap2JNK pathway in 3xTgAD mice. A and B, Cortical lysates from 2.5monthold wildtype (WT) and 3xTgAD (TG) mice were incubated with RalGDSRBD agarose beads, followed by immunoblotting for Rap2 (A) or Rap1 (B), respectively. C and D, Quantification of active Rap2 (C) and active Rap1 (D) levels normalised to total Rap2 and Rap1 respectively ( =9; 6 males, 3 females). E and F, Cortical lysates from 2.5monthold wildtype (WT) and 3xTgAD (TG) mice were collected and subjected to western blotting for activation (phosphorylation) of JNK (E) and p38 (F). G and H, Quantification of pJNK (G, =6; 5 males, 1 female) and pp38 (H, =6; 5 males, 1 female) levels normalised to total expression. All data are shown as the meanSEM. * 0.05, *** 0.001; twotailed unpaired Student's test",yes
PMC6884579,Figure_8,oa_package/31/38/PMC6884579.tar.gz,"['8a c).', ' 8d, e and Supplementary ', 'AFF2 knockout (KO) reduces RNA foci and DPR proteins.', 'Rescue of disease phenotypes by AFF2 loss in human neuronsTo examine whether TDP-43 pathology such as cytoplasmic translocation can be detected in C9ORF72 patient iPSC-derived neurons, we used the neurotrophic factor withdrawal approach.']","Fig. 8 knockout (KO) reduces RNA foci and DPR proteins. Representative images of fluorescence in situ hybridization (FISH) analysis of parental and respective -KO neurons with a Cy3-conjugated (G C ) probe. RNA foci are red, neurons are green (MAP2), and the nuclei are blue (DAPI). Percentage of iPSC-derived neurons containing RNA foci labeled with a Cy3-conjugated (G C ) probe. Average number of RNA foci per neuron. Only neurons containing RNA foci were counted. Each data point is the average number of RNA foci in at least 100 neurons from an independent differentiation. Poly(GR) ( ) and poly(GP) ( ) levels in -KO neurons measured by MSD immunoassay. Each data point represents one differentiation ( =3 independent differentiations for all panels except =4 independent differentiations for patient 1 in and =5 independent differentiations for patient 1 in ). Values are means.e.m. * <0.05; ** <0.01; *** <0.001 (one-way ANOVA, Dunnett's multiple comparisons test). Source data are provided as a Source Data file.",yes
PMC6198061,Figure_7,oa_package/c1/4d/PMC6198061.tar.gz,"['26 Importantly, the resulting enterocytes can be kept for prolonged periods and are postmitotic, as observed by strongly reduced Ki-67 staining ( 7A), and thus allowed us to study phenotypic progression.', 'By confocal microscopy, we observed small and irregular-shaped F-actin foci in the cytoplasm of KO organoids after 2 days of VPA/IWP-2 treatment ( 7B and C).', 'High-resolution confocal imaging of p-Ezrin and F-actin staining confirmed the presence of microvilli ( 7D).', 'MVIs were observed in 76% 9% of all organoids ( 7E), and, on average, 24% 9% of all the cells were affected.', 'These vesicles were heterogeneous with regard to their size, shape, and electron density, and some resembled irregular MVIs ( 7F), and on the basal side, MV-like structures were detected.', 'In contrast, frequent MVIs (of various sizes and shapes) were detected in the cytoplasm ( 7G), and often were associated with the apical or basolateral membrane.', 'We found that the MVIs were negative for Stx3 ( 7H).', 'We tested alternative differentiation protocols and found that 5 days of culture under reduced levels of Noggin or R-spondin also resulted in MVIs, albeit with reduced efficiency and progressive organoid degeneration ( 7I).', ' 7Formation of microvillus inclusions in Munc18-2 KO organoids after prolonged enterocyte differentiation.', 'Tracking of MVI breakdown by LifeAct-mCherry signal in Munc18-2 KO organoid (compare with 7A).', 'Tracking of MVI fusion to the apical membrane by LifeAct-mCherry signal in Munc18-2 KO organoid (compare with 7B).', 'Tracking of MVI fusion to the basal membrane by LifeAct-mCherry signal in Munc18-2 KO organoid (compare with 7C).']","Figure6 . ( ) Abundant presence of F-actin structures in KO organoids ( ) 2 days after withdrawal of R-spondin. Z-projected confocal image stacks are shown. mark basal F-actin. ( ) Quantification of F-actin foci per cell. The fraction of cells with 07 aggregates was determined on consecutive confocal sections in n= 8 representative organoids per condition. ( and ) Ultrastructural characterization of differentiated KO organoids. Note the irregular MVI ( ), enlarged lysosome (yellow region), and basolateral MV-like structures (red region). ( and ) Confocal analysis of F-actin aggregates with p-Ezrin ( ; microvilli) and Lamp2 ( ; lysosomes). mark regions of co-localization, mark basal F-actin. : ( ) 25 m, ( and ) 2 m, and ( and ) 10 m. All staining was confirmed in at least 3 independent experiments. LV, lentivirus.",yes
PMC8544166,Figure_43,oa_package/e3/f3/PMC8544166.tar.gz,[],"Figure 43. Eyelid, accessory lacrimal gland (gland of Wolfring) Meibomian gland, H&E.",yes
PMC3046533,Figure_3,oa_package/0a/a5/PMC3046533.tar.gz,['Histological examination of salivary glands.'],"Figure 3 . Salivary gland histology was examined at 19 wks post-vector infusions of mice treated at 7 wks of age (early treatment) or at 11 wks post-vector infusions of mice treated at 16 wks of age (late treatment). Panels show representative H&E staining of salivary gland tissue from mice receiving early treatment with Ad5-LacZ ( = 10) , or Ad5-IL17A ( = 11) ; fluorescent staining and enumeration of B and T cells in Ad5-IL17A treated mice and immunohistochemical staining and enumeration of IL-17A-positive cells in Ad5-IL17A treated mice ; H&E staining of salivary gland tissue from mice receiving late treatment with Ad5-LacZ ( = 10) , or Ad5-IL17 ( = 8) ; and fluorescent staining and enumeration of B and T cells in Ad5-IL17A treated mice and immunohistochemical staining and enumeration of IL-17A-positive cells in Ad5-IL17A treated mice . Black arrows indicate representative lymphocytic infiltrate.",yes
PMC11163448,Figure_1,oa_package/53/c2/PMC11163448.tar.gz,"['Irregular nonenhancement of a segmental pulmonary artery in the left upper lobe was also noted ().', 'CT thorax in systemic arterial phase demonstrated enhancement of this segmental branch in the left upper lobe ().', 'CT pulmonary angiogram (left) demonstrates non-opacifying segmental pulmonary artery (white arrow); on the CT systemic arterial phase angiogram (right), the same segmental pulmonary artery opacifies; background cavitating consolidation is seen in both lungs.']","Figure 1 CT pulmonary angiogram (left) demonstrates non-opacifying segmental pulmonary artery (white arrow); on the CT systemic arterial phase angiogram (right), the same segmental pulmonary artery opacifies; background cavitating consolidation is seen in both lungs.",yes
PMC6085146,Figure_6,oa_package/c7/a4/PMC6085146.tar.gz,"['Indeed, and in agreement with a previous study13, old mice demonstrate reduced brain perfusion by CSF macromolecules as compared to young counterparts (Extended data a, b).', 'Impaired brain perfusion by CSF in old mice was accompanied by a decrease in meningeal lymphatic vessel diameter and coverage, as well as decreased drainage of CSF macromolecules into dCLNs in both females and males (Extended data c f).', 'To further address the effect of aging on meningeal lymphatics, we performed RNA-seq analysis of LECs sorted from the meninges of young-adult and old mice (Extended data g and ', 'injection, AAV1-infected cells expressing EGFP were limited to the pia around the brain, meninges (dura and arachnoid), and pineal gland (Extended data h j).', 'Treatment of young mice with AAV1-CMV-mVEGF-C resulted in a significant increase in meningeal lymphatic vessel diameter, without affecting blood vessel coverage (Extended data k m).', 'The LECs were then gated as CD45 CD31+Podoplanin+ (see Extended data for representative dot plots) and sorted into a 96-well plate containing 100 L of lysis buffer (Arcturus PicoPure RNA Isolation Kit, Thermo Fisher Scientific) using the Influx Cell Sorter (BD Biosciences) that is available at the University of Virginia Flow Cytometry Core Facility.', 'Extended Data Characterization of meningeal lymphatics in young and old mice and improvement of lymphatic function by viral-mediated expression of mVEGF-Ca, OVA-A647 was injected into the CSF (i.']","Extended Data Figure 6 Characterization of meningeal lymphatics in young and old mice and improvement of lymphatic function by viral-mediated expression of mVEGF-C OVA-A647 was injected into the CSF (i.c.m.) of young-adult (3 months of age) and old (2024 months of age) mice. Representative brain sections stained with DAPI (blue) showing degree of OVA-A647 (red) influx into the parenchyma (scale bar, 5 mm; inset scale bar, 2 mm). Quantification of OVA-A647 area fraction (%) in brain sections (mean s.e.m., = 6 in 3 months, = 8 in 2024 months; two-tailed Mann-Whitney test; representative of 2 independent experiments). Representative images of DAPI (blue) and LYVE-1 (green) staining in meningeal whole-mounts of young-adult (2 months-old) and old (2024 months-old) male and female mice (scale bar, 1 mm). Measurement of LYVE-1 vessel diameter and area fraction showed a significant decrease in both parameters in old mice, when compared to young-adults, in both females and males. Representative images of DAPI (blue) and LYVE-1 (green) staining in dCLNs 2 h after injection of OVA-A594 (red) into the CSF of young-adult and old mice from both genders (scale bar, 200 m). Quantification of OVA-A594 area fraction (%) in the dCLNs of mice from different ages and genders showed a significant decrease in 2024 months-old female and male mice. Data in and is presented as mean s.e.m., = 9 per group at 2 months, = 7 per group at 2024 months for male and female; two-way ANOVA with Bonferronis post-hoc test was used in and ; data was pooled from 2 independent experiments. Representative dot and contour plots showing the gating strategy used to isolate meningeal lymphatic endothelial cells (LECs) by fluorescence-activated cell sorting (FACS) from the meninges of young-adult and old mice ( = 3 per group, pooled from 2 independent experiments). Adult mice were injected i.c.m. with 2 L of AAV1-CMV-EGFP (EGFP) or AAV1-CMV-mVEGF-C (mVEGF-C), both at 10 genome copies (GC)/mL, and transcardially perfused with saline 2 or 4 weeks later. Representative brain coronal sections of mice showing EGFP infected cells (green) in the pia mater, surrounding the GFAP glia limitans (red) of the brain parenchyma, at 2 and 4 weeks post injection (scale bar, 5 mm; inset scale bar, 200 m). Representative insets from meningeal whole-mounts stained for CD31 (blue), EGFP (green) and LYVE-1 (red; scale bar, 200 m). Green cells are observed in the cerebellar meninges, pineal gland and transverse sinus in the EGFP group at 2 and 4 weeks, but not in the same regions of the meninges in the mVEGF-C group. Representative images of LYVE-1 lymphatic vessels (red) and LYVE-1 CD31 blood vessels (blue) in the superior sagittal sinus of mice treated with either EGFP or mVEGF-C, for 2 or 4 weeks (scale bar, 200 m). Mice treated with AAV1 expressing mVEGF-C presented a significant increase in ( ) lymphatic vessel diameter, but not in ( ) coverage by blood vessels. Data in and is presented as mean s.e.m., = 4 per group at 2 weeks, = 3 per group at 4 weeks; two-way ANOVA with Bonferronis post-hoc test was used in and ; data in is representative of 2 independent experiments. Representative images of blood flow (mm/s) and arterial and venous blood oxygenation (% of sO ) readings obtained by Photoacoustic imaging of brain/meningeal vasculature of old mice (2022 months-old) treated for 1 month with EGFP or mVEGF-C virus (both at 10 GC/mL). The different treatments did not affect ( ) blood flow or ( ) blood oxygenation in the brain/meninges of old mice (mean s.e.m., = 5 per group; two-tailed Mann-Whitney test was used in and two-way ANOVA with Bonferronis post-hoc test was used in ; data results from a single experiment). Old mice (2022 months-old) were injected i.c.m. with 2 L of viral vectors expressing EGFP or mVEGF-C. One month later, T1-weighted MRI acquisition was performed after i.c.m. injection of 5 L of gadolinium (25 mM in saline). Using the Lymph4D software, it was possible to measure the rate of contrast agent influx into the delineated brain hippocampal region of mice from both groups (scale bar, 3 mm). Images in sequence 2 and subsequent were obtained by subtraction of sequence 1. Quantification of the signal intensity gain (relative to sequence 1) in the hippocampus revealed a significant increase in the mVEGF-C group, when compared to EGFP (mean s.e.m., = 4 per group; repeated measures two-way ANOVA with Bonferronis post-hoc; data was pooled from 2 independent experiments).",yes
PMC3141762,Figure_1,oa_package/13/2d/PMC3141762.tar.gz,['Photomicrographs showing immunohistochemistry for estrogen and progesterone receptors.'],Figure 1 . (A and B) Epithelioid cells in both primary and metastatic foci were negative for estrogen receptors ( 400). (C) Pancreatic islet cells showed a weakly positive reactivity for estrogen receptos ( 400). (D and E) Epithelioid cells in both primary and metastatic foci showed a strongly positive reactivity for progesterone receptors ( 400). (F) Pancreatic islet cells showed a strongly positive reactivity for progesterone receptors ( 400).,yes
PMC6580335,Figure_3,oa_package/4a/36/PMC6580335.tar.gz,['Photomicrographs of the trabecular type of basal cell adenoma.'],"Figure 3 Photomicrographs of the trabecular type of basal cell adenoma. A: A capsule and an eosinophilic basal membrane-like structure (hematoxylin-eosin staining, 40); B: Small cuboidal to columnar epithelial cells arranged in strands with round hyperchromatic nuclei and relatively rounded central cells. The strands are surrounded by hyalinised connective tissue (hematoxylin-eosin staining, 100).",yes
PMC9272948,Figure_1,oa_package/44/31/PMC9272948.tar.gz,"['CT scan was done and revealed bilateral and diffuse patchy ground glass opacity and lower lung bronchiectasis ().', 'CT scan showing bilateral ground glass opacity and bronchiectasisSections reveal dilated lung alveolae, type II pneumocytes hyperplasia.']",Figure 1 CT scan showing bilateral ground glass opacity and bronchiectasis,yes
PMC7732650,Figure_2,oa_package/77/91/PMC7732650.tar.gz,"['Brain MRIs of subject no.', '5 ().', '5 starting at the age of 10 ().']","Figure 2 Brain MRIs of subject no. 5. In the left part of the figure, MRIs of three different levels (deep nuclei, centrum semiovale, and middle cerebellar peduncles) are displayed. Under the age of 3 years, only a very mild T2 hyperintensity is evident at the level of the periventricular WM. Subsequent MRI scans show a progressive marked diffuse T2 hyperintensity of the WM also involving the posterior limb of the internal capsule (PLIC) as well as the external and the extreme capsule bilaterally. Infratentorially, only a mild T2 hyperintensity is evident at the age of 6 years 3 months at the level of the dorsal pons that becomes more pronounced at the age of 10 and 1416 years. Magnification of axial T2 image acquired after the age of 16, showing a remarkable involvement of the U fibers (arrow). ( Coronal T2 image acquired at the age of 16 years showing involvement of temporal WM (arrow) and also T2 hyperintensity of corticospinal tracts. Magnification of axial T2 images at the age of 16 showing T2 hyperintensities at the level of the tail of the putamen (arrowhead) and the level of the pulvinar (arrow).",yes
PMC5110013,Figure_2,oa_package/84/b7/PMC5110013.tar.gz,"['To determine the effect of hypoxia, we altered this procedure by exposing cells either to hypoxia or normoxia at the embryoid body (EB) stage and studied the differences among CMS and non-CMS cells ().', ' shows a remarkable difference in response to hypoxia among the three different groups.', 'On the other hand, CMS subjects had a marked response, increasing to 57%, corresponding to a huge increase of many folds ().', ' (A and B) shows the robust response of each of the CMS (n = 5), non-CMS (n = 4), and sea-level (n = 3) subjects (***, P 0.', 'Furthermore, to determine interclonal variability, we have now tested three clones for each of the three subjects in each group under hypoxia ( C).', '99; C).', '.', 'This in vitro experiment replicated the high-altitude in vivo response of the three populations.', 'With these Sendai-generated iPSCs, we observed a similar pattern of phenotypic differences in hypoxia: an exuberant response in CMS and a blunted one in non-CMS cells ( A).', ' D shows the differential sensitivity of the three populations to graded hypoxia, with a huge sensitivity to hypoxia in cells from CMS subjects.']","Figure 2. (A) Flow cytometric analysis using CD235a (glycophorin A) as a marker after culturing as detailed in at the EB stage at day 28. Sendai virus results: Representative FACS plots of sea level, non-CMS, and CMS in normoxia (left) and in hypoxia (right). The dot plot represents the live cells as gated through propidium iodide. CD235a cells are shown in red along the y axis, and CD235a cells are shown in blue. The percentage in each figure represents the relative proportion of CD235a cells. There are major differences between CMS (bottom) versus the non-CMS (middle) and sea-level (top) samples. The FACS plots are representative of one experiment. Similar results were obtained in all the experimental repeats. (B) Summary of hypoxic response of CMS patients ( = 5 subjects) and non-CMS ( = 4 subjects) and sea-level ( = 3 subjects) control subjects. The graph depicts the relative proportion of CD235a quantified 3 wk after the administration of hypoxia (5% O ). There is a significantly striking difference between sea level, non-CMS, and CMS under hypoxia. ***, P < 0.001. Error bars represent the mean SEM of at least two to three measurements. The experiment was repeated at least three times. (C) Summary of interclonal variability among the subjects: three clones (clones 1, 2, and 3) were tested for three subjects (subjects 1, 2, and 3) for each group: CMS, non-CMS, and sea level. The y axis depicts the relative proportion of CD235a under hypoxia for different clones. Error bars represent the mean SEM of at least two to three measurements. (D) Dose response. The graph represents the response (as measured by proportion of CD235a [y-axis]) of CMS, non-CMS, and sea-level cells to 21%, 10%, 5%, and 1.5% O levels (x axis). Each point depicts the mean SEM of at least two to three measurements. CMS shows hyperresponsiveness at 10, 5, and 1.5% O . The experiment was repeated at least three times.",yes
PMC11526661,Figure_4,oa_package/52/13/PMC11526661.tar.gz,"[' 4A; Supplementary Table 11).', ' 4B; Supplementary Table 12).', '\nAssociation of candidate proteins with incident all-cause, Alzheimer s, and vascular dementia in the UK Biobank.', 'A bolded protein name indicates significant effect modification by APOE 4 genotype in the WHIMS discovery analyses\nSecondary analyses examined protein associations with incident AD and VaD, two dementia subtypes defined based on suspected etiology.', ' 4A; Supplementary Table 11).', ' 4A; Supplementary Table 12).']","Fig. 4 Association of candidate proteins with incident all-cause, Alzheimers, and vascular dementia in the UK Biobank. . Association of 4 candidate proteins with incident dementia in the UK Biobank. . Association of 3 candidate proteins with incident dementia in the UK Biobank. Hazard ratios were derived from Cox proportional hazards models adjusted for age, sex, education study site, BMI, kidney function (eGFR), diabetes, and cholesterol. Pink circles represent statistical significance at an unadjusted <0.05. A bolded protein name indicates significant effect modification by 4 genotype in the WHIMS discovery analyses",yes
PMC9428033,Figure_1,oa_package/70/89/PMC9428033.tar.gz,"['Representative staining patterns obtained in bronchopulmonary (A C) and gastroenteropancreatic neuroendocrine neoplasms (D F) using the antibody against somatostatin receptor 4 (SST4) 7H49L61, showing no (A, D) or moderate (B, C, E, F) SST4 expression intensity.', 'The staining of SST4 in the tumours was assessed using the semiquantitative immunoreactivity score (IRS) according to Remmele and Stegner (1987)37.', 'ResultsSST4 expression patterns 1 shows representative images of BP-NEN and GEP-NEN samples stained with the anti-SST4 antibody 7H49L61.', ' 1).']","Figure 1 Representative staining patterns obtained in bronchopulmonary ( ) and gastroenteropancreatic neuroendocrine neoplasms ( ) using the antibody against somatostatin receptor 4 (SST4) 7H49L61, showing no ( , ) or moderate ( , , , ) SST4 expression intensity. Immunohistochemistry (red-brown colour), counterstaining with haematoxylin. Scale bar: 50m. NEN I, neuroendocrine neoplasm 1; NEN II, neuroendocrine neoplasm 2; SCLC, small-cell lung cancer.",yes
PMC3088788,Figure_3,oa_package/a5/0e/PMC3088788.tar.gz,"[' 3a,b shows the morphology of cultured sepsis-APC by conventional Wrights Giemsa staining, and similar to the fluorescence microscopy, the autophagy vacuoles appeared empty.', ' 3c,d).', ' 3c,d).', 'Cytoplasm containing multivesicular bodies (mvb) and mitochondria (m) in various states of structural disintegration (c, bar: 500 nm)); enlarged mitochondria (d, bar: 100 nm)\n']","Fig.3 Autophagy in 21-day cultured sepsis-APC. Vacuolar structures (Wrights Giemsa stain: , ). Cytoplasm containing multivesicular bodies (mvb) and mitochondria (m) in various states of structural disintegration ( , bar: 500nm)); enlarged mitochondria ( , bar: 100nm)",yes
PMC11244770,Figure_3,oa_package/86/d7/PMC11244770.tar.gz,"['Compared with the doxorubicin group, NLRP3, caspase-1, GSDMD and IL-18 in the icariin group and prednisone group showed a downward trend (as shown in ).', 'g003Icariin alleviates the levels of inflammatory cells in the nephrotic syndrome rat induced by doxorubicin.']",10.1371/journal.pone.0298353.g003,yes
PMC3189583,Figure_2,oa_package/e7/aa/PMC3189583.tar.gz,"['Several studies using primarily cell culture systems have shown that the DGC may be capable of participating as a scaffold for various signal transduction cascades ().', '001""/>The dystrophin-glycoprotein complex may participate in laminin-dependent signaling in skeletal muscle.']","Figure 2 The dystrophin-glycoprotein complex may participate in laminin-dependent signaling in skeletal muscle. Interactions shown in blue indicate interactions that have been shown to be increased when laminin is bound to dystroglycan. Interactions in pink indicate those that have been shown to be increased following a muscle contraction protocol. Since the phosphorylation of -dystroglycan can bind a number of other SH2-domain containing proteins and can also interact with Grb2, it may participate in additional signal transduction cascades that have not yet been identified. It is important to remember that in many cases, these interactions have been studied in cell culture systems and the relevance of these interactions in muscle has not been extensively studied.",yes
PMC6580697,Figure_6,oa_package/06/75/PMC6580697.tar.gz,"['Once the stricture has been crossed, dilators or a balloon catheter can be used to dilate to 9 French, allowing the placement of ureteric stent (a,b).', 'g005""/>.']","Figure 6. Subsequent placement of ureteric stent (6a), and (6b) Fluoroscopy demonstrates formation of the distal pigtail, confirming correct positioning of the distal tip of the stent within the urinary bladder.",yes
PMC11526003,Figure_3,oa_package/89/df/PMC11526003.tar.gz,"[' 3).', 'Tradeoff between resolution and FOV addressed with user-selected FOV in freehand, live skin imaging.', 'Validation of clinical performance in living, normal human skin: measures of dermal elastosis and epidermal pigmentationTo evaluate cross-modal s in vivo performance, we conducted noninvasive imaging on 122 human participants throughout the development of the technology (Supplementary Table S1).']","Fig. 3 Tradeoff between resolution and FOV addressed with user-selected FOV in freehand, live skin imaging. In a single session of freehand imaging of living skin, the user captured an image of interest with ( ) a FOV optimized for signal-to-noise ratio for improved clarity of small features (zoom; 133m100m). Cell nuclei in the epidermis and thin fibrils in the dermis are resolved. Variations in cytoplasmic color from green to yellow indicate variations in melanin concentration. White circles, 1m diameter. ( ) While freehand imaging the same region of skin (approximate location of dotted rectangle), the user captured a larger FOV (standard; 400m300m), which captures a different view and context of histologic features. Scale bars, 100m.",yes
PMC5626538,Figure_7,oa_package/e6/85/PMC5626538.tar.gz,"['Analysis of amyloid plaque pathology via LAMP1 and A immunofluorescence revealed a striking increase in overall plaque burden (area occupied by plaques) in the cerebral cortex of JIP3+/ ; 5xFAD mice compared with their respective gender-matched 5xFAD control littermates (, A and C).', 'Additionally, examination of individual plaques revealed a greater mean area per plaque that was occupied by axonal dystrophies (LAMP1 staining) and amyloid deposits (A staining; B).', 'These observations are supported by quantification of the mean area occupied by each of these markers per plaque (, D and E; and ', 'There was also an increased number of lysosome-filled axonal swellings per plaque ( F and ', 'Lastly, in addition to the worsened amyloid plaque pathology, there were also increased amounts of soluble A 42 in JIP3+/ ; 5xFAD brains compared with their 5xFAD littermates ( G).', 'Consistent with their genotype, JIP3 heterozygous 5xFAD mice had half the levels of JIP3 protein of their 5xFAD littermates ( H).', '.', 'Our neuron primary culture and in vivo data are consistent with the conclusion that JIP3 depletion results in intraneuronal APP processing and A 42 generation.']","Figure 7. (A) Lysosomes (LAMP1, green) and amyloid plaque (A, red) labeling in stitched confocal images of the cerebral cortex of 5xFAD and JIP3+/; 5xFAD littermates. Bars, 100 m. (B) High-magnification images of A deposits and their surrounding lysosome-filled dystrophic axons from 5xFAD and JIP3+/; 5xFAD littermates. Bars, 10 m. (C) Quantification of the plaque burden (area occupied by plaques, normalized to 5xFAD sample) in three pairs of 5xFAD controls and JIP3+/; 5xFAD gender-matched littermates. ***, P < 0.001, unpaired test. (D) Area of individual plaques as defined by A signal in JIP3+/; 5xFAD animals normalized to their corresponding gender-matched 5xFAD littermates ( = 5 pairs of gender-matched littermates, 4550 plaques measured per animal per genotype; ***, P < 0.001, unpaired test). (E) Quantification of the area of individual dystrophic axon cross sections (LAMP1 signal) in JIP3+/; 5xFAD animals normalized to their corresponding gender-matched littermates (4550 accumulations per genotype per animal were analyzed; **,P < 0.01, unpaired test). (F) Cumulative frequency distribution of the number of axonal swellings per plaque in both 5xFAD and JIP3+/; 5xFAD littermates (***, P < 0.001, KolmogorovSmirnov test). (G) Quantification of soluble A42 (outside of plaques) levels in brain homogenates from three pairs of 5xFAD and JIP3+/; 5xFAD gender-matched littermates (**, P < 0.01, unpaired test). (H) Quantification of JIP3 protein levels from three pairs of 5xFAD and JIP3+/; 5xFAD littermates (***, P < 0.001, unpaired test). Data presented as mean SD in CE, G, and H.",yes
PMC6663160,Figure_2,oa_package/84/3d/PMC6663160.tar.gz,"['Twenty non-sclerotic glomeruli showed membranoproliferative glomerulonephritis (MPGN) with lobular accentuation, and endocapillary proliferative change was also noted in several glomeruli (figure 2A,B).', 'Higher magnification revealed hollow-centred microtubular structures of 35 45 nm diameter in these deposits, confirming the diagnosis of ITG (figure 2C,D).', '(A,B) Light microscopic findings from the second biopsy showed membranoproliferative glomerulonephritis with lobular accentuation, and endocapillary proliferative change.']","Figure 2 (A,B) Light microscopic findings from the second biopsy showed membranoproliferative glomerulonephritis with lobular accentuation, and endocapillary proliferative change. (A) Periodic acid-Schiff.400. (B) Periodic acid-Schiff-methenamine silver.400. (C,D) Electron microscopic findings showed dense deposits in the mesangial areas and subendothelial space. Higher magnification revealed hollow-centred microtubular structures of 3545nm diameter in these deposits. (C) The white line corresponds to 2m. (D) The white line corresponds to 500nm.",yes
PMC9977756,Figure_4,oa_package/a7/e5/PMC9977756.tar.gz,[],FIGURE 4 Regional MD and T2 maps of the hippocampus in a representative (A) control (32years old) and two unilateral HS patients. Regions of elevated T2 (above 95ms) were present in the ipsilateral (indicated by *) hippocampus of (B) and (C) and contralateral head of hippocampus of (B). These elevated T2 regions overlapped with regions of high MD and remained consistent between the longitudinal scans. Note that the maps are not scaled to size between patients but are scaled the same left/right,yes
PMC4440933,Figure_2,oa_package/c1/3b/PMC4440933.tar.gz,"['Low-grade DNs show features suggestive of a clonal cell population without significant architectural or cellular atypia (A).', 'The architectural atypia observed in high-grade DNs includes the thick cell plates up to three cells thick and/or pseudoglandular structures (B).', ' The most important histologic feature that distinguishes early HCCs from high-grade DNs is stromal invasion, defined as the presence of tumor cells invading the portal tracts or fibrous septa (C).', 'The common histological patterns include (1) trabecular pattern, where tumor cells with hepatocellular differentiation are arranged in plates of various thickness, separated by sinusoid vascular spaces (D); (2) pseudoglandular pattern, where gland-like dilatation of the canaliculi are present between tumor cells forming the lumen; (3) compact pattern, which is composed of thick trabeculae compressed into a compact mass; (4) scirrhous pattern, where desmoplastic stroma divides tumor cell masses into smaller nests; and (5) fibrolamellar pattern, which shows characteristic lamellar collagen bundles running between large glassy tumor cells.', '.']","Fig. 2. Representative pathologic images of multistep hepatocarcinogenesis. (A) Low-grade dysplastic nodule (right of the dashed line) shows increased cellularity, and residual portal tracts (arrow) are easily identifiable within the nodule. (B) High-grade dysplastic nodule (left of the dashed line) shows further increased cellularity and frequent unpaired arteries (arrow). (C) Early hepatocellular carcinoma (below the dashed line) is poorly demarcated but shows unequivocal cytological atypia and stromal invasion (arrow). (D) Advanced hepatocellular carcinoma is well demarcated by a thick capsule and shows overt features of malignancy.",yes
PMC4947696,Figure_1,oa_package/3e/d8/PMC4947696.tar.gz,"['Examples of nuclear Ki67 and DLX2 staining are shown in .', '19117060Ki67 and DLX2 localisation in prostate cancer.']",Figure 1 Examples of positive Ki67 ( ) and DLX2 ( ) nuclear prostate cancer staining compared with tumours that did not express Ki67 ( ) or DLX2 ( ).,yes
PMC5107605,Figure_1,oa_package/58/d4/PMC5107605.tar.gz,['Imaging examinations.'],"Figure 1 Imaging examinations. The radiography (A) showed right pleural effusion and thickening, and potential interposition of the dilated colon. The colon double contract pneumobarium radiography (B and C) and the abdominopelvic computed tomography (D-F) revealed significant expansion of the cecum, the ascending colon, the hepatic flexure of colon, and the remnant transverse colon, with the most dilated area 13.6 cm wide. Abundant residual stool, gas, and liquid existed. An annular stenotic transitional segment (only 3.8 cm wide) could be perceived. The other intestines were normal. The neighboring organs and tissues underwent marked displacement and deformation under pressure.",yes
PMC4863962,Figure_3,oa_package/da/7f/PMC4863962.tar.gz,"['MuLV infection stimulates DC proliferation and selective maturation of CD19+ DCsCD19+ DCs are a minor DC population in spleens of na ve mice (A, 1% of splenocytes and ~10% of splenic DCs).', 'CD19+ DCs expanded substantially after MuLV infection, accounting for ~10% of splenocytes and ~50% of splenic DCs at 35dpi (B and 3C).', 'Conventional (CD19neg) DCs also expanded in this period, while relative proportions of splenic B cells were reduced substantially (A and 3B).', 'At 35dpi, CD19+ DCs expressed uniformly high levels of MHC class II (MHC II) and CD80 (D) characteristic of mature antigen presenting cells (APCs).', 'In contrast, conventional DCs expressed lower and more variable levels of MHC II and CD80/86 (D) comparable with levels on immature DCs in na ve mice.', 'Higher proportions of splenic DCs stabilized ~28dpi after MuLV infection and in vivo labeling with 5-ethynyl-2-deoxyuridine (EdU) at 14-21dpi revealed larger cohorts of dividing (EdU+) splenic CD19+ (~30%) and conventional (~16%) DCs than B cells ( 5%) from MuLV-infected B6 mice (E and 3F).', 'g003MuLV infection induces selective accumulation, proliferation and maturation of CD19+ DCs in spleen.']",10.1371/journal.ppat.1005615.g003,yes
PMC4111849,Figure_4,oa_package/04/25/PMC4111849.tar.gz,"['As seen in 4, at least 10 novel isoforms exhibited dominant expression when compared with the previously known isoforms in the heart or testes.', '\nAlternative splicing patterns of the novel isoforms containing the novel exons.', 'Conversely, the novel isoforms for Pkm2, Ms4a5, Pfkm, and Skp2 were expressed at relatively low levels in the testes.']","Figure 4 Multiple isoforms either with or without the novel exons were produced by RT-PCR. Exon structures amplified by the primers are seen in the right panel, while novel exons are highlighted with blue boxes. The relative expression levels of the isoforms were quantified by ImageJ.",yes
PMC11570843,Figure_3,oa_package/e2/9a/PMC11570843.tar.gz,"['There are 5 profiling tools on the DSP: geometric, gridded, gradient, segmentation, and cell-type specific ( 3).', ' 3Distinct approaches to select ROIs for in situ profiling.', 'In gridded profiling, the sample is divided by uniformly spaced, equally size geometric ROIs ( 3).', '4"">Contour profilingFor studies investigating the heterogeneity of biological gradients such as chemokines, contour profiling may be useful ( 3).']","Figure3 There are 5 modalities of ROI selection, including geometric, segmentation, cell-type specific, contour, and gridded. An example of the same psoriasis tissue image used for demonstration is provided. ROI. region of interest.",yes
PMC3851716,Figure_1,oa_package/44/4f/PMC3851716.tar.gz,"['2005), we observed a considerably altered cell shape and actin organization when compared to Fmr1+ MEFs re-expressing the protein (A,B).', 'These experiments revealed that Fmr1 cells showed an 25% reduction in F-actin/G-actin ratio compared to cells re-expressing the protein (C).', 'To further exclude bias by clonal variability, we analyzed whether FMRP expression in Fmr1 cells after lentiviral transduction without clonal selection rescues the changes in actin organization.', 'Expression of FMRP-EGFP in the Fmr1 cells induced an increase in stress fibers and an elongated cell shape along with a redistribution of focal contacts which was not observed after the expression of EGFP alone (D,E).', 'The expression of FMRP-EGFP increased F-actin/G-actin ratios by 25% (F).']","FIGURE 1. The loss of FMRP alters the morphology and F-actin/G-actin ratios of MEFs. ( ) Immunofluorescence studies of FMRP re-expressing MEF clones ( +, clones 56, 59, panels) with parallel stress fibers and cell clones (clones 81, 87, panels) with reduced stress fiber formation. Enlargements of boxed regions are shown in the panels; bar, 20 m. ( ) Representative Western blotting of and cell lysates confirming FMRP loss in the cell clones and FMRP re-expression in clones 56 and 59. ( ) F-actin/G-actin assay of and cell clones showing a reduced F-actin/G-actin ratio in cell clones. The F-actin organization of both cell clones (81 and 87) was compared to both WT cell clones re-expressing FMRP (56 and 59). = 3, (*) 0.05. ( ) Fluorescence microscopy studies of cells after lentiviral transduction with the indicated constructs showing F-actin- ( panels) and vinculin-staining ( panels). Re-expression of FMRP (FMRP-EGFP) reverted the phenotype compared to control transduction (EGFP); bar, 20 m. ( ) Representative Western blotting of cell lysates after lentiviral transduction with FMRP-EGFP or EGFP alone confirming FMRP re-expression in cells. ( ) F-actin/G-actin assay of lentivirally transduced MEFs showing an increased F-actin/G-actin ratio in FMRP-EGFP-expressing cells. = 3, (*) 0.05.",yes
PMC3698899,Figure_4,oa_package/8d/99/PMC3698899.tar.gz,"['[1] Benign vascular lesions like hemangiomas can be effectively treated by embolization and surgery may be avoided [].', '(A-H)Direct puncture sclerotherapy of tongue base slow flow vascular malformation.']","Figure 4(A-H) Direct puncture sclerotherapy of tongue base slow flow vascular malformation. A 21-year-old female with a large slow flow vascular malformation of the tongue base presented with dysphagia and difficulty in speech. (A) Axial, (B) Sagittal T2W images shows a hyperintense lesion (arrow) in the base of tongue causing marked narrowing of oropharynx (arrow head). (C) Arterial, (D) Venous phase of Lingual artery (arrow) angiogram shows progressively increasing and persistent vascular blush in the lesion (arrow) suggestive of slow flow vascular malformation. Direct puncture sclerotherapy was performed through percutaneous (E) under sonographic guidance and transoral (F) route (Black arrow) using STS. Using expandable suspending laryngoscope, the base of tongue can be optimally visualized and direct puncture of the lesion can be performed by transoral route. (G) Axial, (H) Sagittal fat suppressed contrast T1W images done after 6 weeks shows significant reduction in the size and enhancement of residual malformation (arrow) with good clinical improvement in symptoms",yes
PMC10466255,Figure_7,oa_package/18/49/PMC10466255.tar.gz,"['The tendon is then cinched down onto the suture anchor under arthroscopic visualization and tied into place using an arthroscopic knot pusher (, A and B).', 'Viewing a left shoulder through the posterior portal in beach chair position with the arm abducted and externally rotated (A), an arthroscopic knot pusher is used to tie a knot to secure the long head of the biceps (LHB) (B).']","Fig 7 Viewing a left shoulder through the posterior portal in beach chair position with the arm abducted and externally rotated (A), an arthroscopic knot pusher is used to tie a knot to secure the long head of the biceps (LHB) (B). (B) The LHB (B) is secured after a knot has been placed.",yes
PMC5818378,Figure_6,oa_package/85/cb/PMC5818378.tar.gz,"[' 6; [37]).', '[40]Competitive sports activity increases the risk of SCD by 5 fold in adolescent and young adults with ARVC [5].']","Fig. 5 Electrocardiographic and echocardiographic findings in a14-year-old male soccer player with right ventricular dominant (classic phenotype) arrhythmogenic cardiomyopathy. The electrocardiogram shows Twave inversion in right precordial leads (V1V2)( ). The echocardiogram reveals right ventricular dilatation (right ventricular outflow tract diameter of 39mm on end-diastolic parasternal short-axis view)( ) and right ventricular dysfunction (akinesia of right ventricular outflow tract and posterobasal, subtricuspidal regions) (not shown). Reproduced with permission from Migliore etal. [ ]",yes
PMC5498912,Figure_7,oa_package/9a/b5/PMC5498912.tar.gz,"['006""/>Inhibitory effects of FSCP on TNF- -induced activation of the NF- B signaling pathway.']",Figure 7 Inhibitory effects of FSCP on TNF- -induced activation of the NF- B signaling pathway. Western blot analysis showed NF- B p65 levels were decreased in the cytoplasm and increased in the nucleus in TNF- -treated HaCaT cells (a). Immunofluorescence microscopic assay revealed that FSCP prevented the translocation of NF- B p65 to the nucleus in TNF- -treated HaCaT cells (b). Results are presented as the meansSD of three independent experiments. CE: cytoplasmic extracts; NE: nuclear extract. < 0.05 versus the control and < 0.01 and < 0.001 versus the TNF- -treated group.,yes
PMC8387157,Figure_1,oa_package/a0/70/PMC8387157.tar.gz,"['Multicomparisons were corrected with Dunnett s post hoc test when comparing three or more groups (for details, see Extended Data -2).', 'Equivalent overexpression was confirmed by Western blotting for Syn WT and 3K (A).', 'Cell viability was assessed by measuring ATP levels and was significantly reduced by 40% 19 d after AAV9 Syn 3K compared with EV AAV9 transduction (B).', 'A clear trend toward cytotoxicity was observed after 12 but not 6 d with Syn 3K (B).', 'In contrast, only minor or no cytotoxicity was observed with Syn WT in this system (B).', 'Dose-dependent and time-dependent cytotoxicity was observed in rat and mouse cortical neuron cultures, where mouse cultures showed greater sensitivity to Syn 3K stress (Extended Data -1).', 'Of note, we found Syn 3K to be hyperphosphorylated at S129 in comparison to Syn WT, and mutation of the phosphorylation site (S129A) did not reduce cytotoxicity (A,B).', '.', 'Dose-dependent and time-dependent toxicity analysis in mouse and rat cortical neuron cultures are shown in Extended Data -1.', 'Extended Data -2 displays additional information on statistical tests applied in present study.', '2021_f001""/>10.', 'f1-1Extended Data -1 Syn WT versus 3K toxicity in rat and mouse cortical neuron cultures.', 'tif"" content-type=""local-data"">-1, TIF file.', '10.', 'f1-2Extended Data -2Statistical table.', 'xls"" content-type=""local-data"">-2, XLS file.', 'Principal component analysis of the gene expression profiling in our primary rat neuron cultures showed the greatest transcriptional changes between different culture ages (6 vs 12 vs 19 d after AAV9; C).', 'Furthermore, the expression of endogenous Syn increases in the neuron cultures through time (E), which has been reported in primary neuron maturation previously (Courte et al.', 'Separation of EV and Syn 3K groups began at 12 d after transduction (E).', 'Among these top hits was SCD1, which was downregulated by Syn 3K (D).', 'We also confirmed SCD1 downregulation by Syn 3K by qPCR (F).', 'The SCD2 isoform, which has high expression in the rodent brain, was also down regulated by Syn 3K (Extended Data -1E; Kaestner et al.', 'SCD1 expression decreases overtime in these cultures suggesting that they become less dependent on SCD and OLA as they mature (D).', 'Further supporting this differential requirement of MUFAs is that the expression of SCD1 reduces through time in primary neuron cultures, dropping by 50% every 6 d between early and late cultures (D).', 'The down regulation of SCD1 and SCD2 (D; Extended Data ']","Figure 1. RNA-Seq identifies SCD as a Syn 3K neurotoxicity regulated gene in rat cortical neuron cultures. , Western blotting showing equivalent expression of Syn WT, Syn 3K, or Syn 3K S129 phosphorylation mutant (S129A) in rat cortical neuron cultures on respective AAV transduction. , Viability assessed by ATP levels (CellTiter-Glo) in rat cortical neuron cultures under respective AAV9 transduction, two-way ANOVA with Dunnetts multiple test correction. Dose-dependent and time-dependent toxicity analysis in mouse and rat cortical neuron cultures are shown in Extended Data . , Principal component analysis of RNA-Seq data from rat cortical neurons with AAV9-EV or AAV9-Syn 3K (6, 12, and 19 d after AAV9 transduction). , Relative transcripts per million of SCD1 transcripts in AAV9-EV and AAV9-Syn 3K samples. , Relative transcripts per million of endogenous rat SNCA (Syn) transcripts in AAV9-EV and AAV9-Syn 3K samples. , qPCR confirmation of SCD1 transcript suppression from AAV9-Syn 3K in rat cortical neuron cultures 12d after AAV9 transduction, two tailed test. Data displayed as boxplots or as line charts showing error bars with SD; * < 0.05, ** < 0.01, *** <0.001. Extended Data displays additional information on statistical tests applied in present study.",yes
PMC9754727,Figure_2,oa_package/60/2f/PMC9754727.tar.gz,"[' Triple phase CT of the liver following TJLB(A) Unenhanced CT showing hyperdense fluid around the liver (red arrows), surrounded by less prominent hyperdense fluid (blue arrows), indicating intraperitoneal haemorrhage with sedimentation of blood into layers.']","Figure 2 Triple phase CT of the liver following TJLB (A) Unenhanced CT showing hyperdense fluid around the liver (red arrows), surrounded by less prominent hyperdense fluid (blue arrows), indicating intraperitonealhaemorrhagewith sedimentation of blood into layers. (B) Arterial phase CT showing arterial phase contrast in the aorta (red circle) and hepatic arteries (red arrow) with no acute extravasation of contrast detectable around the liver. (C) Venous phase CT showing portal venous phase contrast in the aorta (red circle) and hepatic portal veins (blue arrow) with no acute extravasation of contrast detectable around the liver. TJLB: Transjugular Liver Biopsy",yes
PMC4065024,Figure_1,oa_package/ba/2e/PMC4065024.tar.gz,"['ResultsRNA fluorescence in situ hybridizationThe presence of RNA foci clearly distinguished fibroblasts, lymphoblastoid cells and CNS tissue from C9orf72+ patients with ALS compared to C9orf72 patients with ALS and neurologically normal control subjects (A D).', 'RNase treatment in fibroblasts ablated foci, illustrating that the labelled product is RNA and in agreement with previous studies (A).', 'RNA FISH shows GGGGCC expanded RNA foci are found in peripheral cells and CNS tissue from C9orf72+ patients but not from C9orf72 ALS cases or controls.', 'It is noteworthy that RNA foci were identified in fibroblasts derived from an asymptomatic C9orf72+ carrier (C).', 'In four C9orf72+ cases the proportion of foci+ cerebellar granule neurons was quantified and compared to the proportion of foci+ motor neurons in the ventral horn (E).', '1093/brain/awu120/-/DC1"">Supplementary ); in these patients the average proportion of foci+ neurons was significantly higher in the ventral horn (61% versus 27%, t-test P 0.', 'Foci were primarily nuclear, however, some cytoplasmic foci were also observed in fibroblasts, cerebellar granule cells and in motor neurons (C).']","Figure 1 RNA FISH shows GGGGCC expanded RNA foci are found in peripheral cells and CNS tissue from + patients but not from ALS cases or controls. RNA foci (arrowheads) are present in fibroblasts ( ), lymphoblastoid cells ( ) and CNS tissue ( ) from + patients with ALS, and in fibroblasts from a + asymptomatic carrier ( ). RNA foci are ablated by RNase treatment ( ). RNA foci are predominantly nuclear but cytoplasmic foci are observed in peripheral cells and CNS tissue ( and , arrows). Abundance of foci in cerebellar granule cells and motor neurons has been quantified ( ), in those cases where the initial clinical presentation was ALS the proportion of foci+ motor neurons is significantly higher (* 0.05). Scale bar = 3 m. FTD = frontotemporal degeneration.",yes
PMC6758702,Figure_1,oa_package/80/a3/PMC6758702.tar.gz,"['Positive SV40 staining is used to confirm the diagnosis of BKVN () [13,14].', '.']","Figure 1. (A) Light microscopy image of BK virus renal allograft nephropathy. The histological manifestations are characterized by nuclear inclusion bodies in tubular epithelial cells (arrow indicates the hematoxylin-eosin-stained paraffin section, 400). (B) Immunostaining of BK virus-infected cells with anti-SV40 large T antigen antibodies showing the nuclei of renal tubular epithelial cells have a transparent center and thorn-shaped periphery (arrow indicates the immunohistochemical staining, 400).",yes
PMC6192729,Figure_2,oa_package/28/31/PMC6192729.tar.gz,"['Edge Z-score distributions of the various subgroups of GC are presented in A.', 'The overlap of confident edges among the three subtypes of GC was displayed as a Venn diagram (B); 85% of TF-target edges were shared among all three subtypes, indicating that the TF-target relationship was strongly conserved.', 'According to the definition of DF-specific edges (ModuleDF), IT-specific edges (ModuleIT) and commonly conserved edges, different edges were marked with different colors in a 3D scatter plot, in which each axis represented a Z-score of each subtype of GC (C).', 'D exhibited the projection of this 3D plot through each axis.', 'The overlap of differentially expressed genes between DF and IT GC, as well as the target genes of ModuleDF and ModuleIT were illustrated in a Venn diagram (E).', 'By applying the hypergeometric distribution model to the target genes of each TF, it was revealed that most TFs with a high activity in ModuleDF also had a high activity in ModuleIT (F).', 'Gene regulatory network construction, and DF- and IT-specific TF-target edge identification.']","Figure 2 Gene regulatory network construction, and DF- and IT-specific TF-target edge identification. (A) Edge Z-score distribution in different subgroups of GC. (B) Overlap of TF-target edges among the different subgroups of GC. (C) Identification of DF- and IT-specific TF-target edges in a 3D scatter plot, exhibiting Z scores of DF, MX and IT GC. Low confidence edges were colored gray, conserved edges were colored purple, DF-specific edges were colored red, IT-specific edges were colored blue and other edges were colored green. (D) Projection of 3D view through the y-axis. (E) Overlap of target genes in ModuleDF/ModuleIT and DF/IT differentially expressed genes. (F) Overlap of TFs enriched in DF-specific TF-target edges with DF differentially expressed genes (ModuleDF) and IT-specific TF-target edges with IT differentially expressed genes (ModuleIT). DF, diffuse; GC, gastric cancer; IT, intestinal; MX, mixed; TF, transcription factor.",yes
PMC10690915,Figure_2,oa_package/d8/c6/PMC10690915.tar.gz,"['3 In the classic form (common pattern) (A-G), hallmark cells are numerous and form cohesive sheets.', '27\nDUSP22-R ALK ALCL (H-N) frequently demonstrate doughnut-shaped nuclei and, compared to DUSP22 non-rearranged (NR) cases, are more frequently CD3+ but less commonly express EMA and cytotoxic molecules.', '32.', 'fig2"" position=""float""/>Other gene fusions reported in ALK ALCL involve the tyrosine kinase domain of FRK, ROS1 or TYK2.']","Figure 2. (A-G) Anaplastic lymphoma kinase (ALK)-positive anaplastic large cell lymphoma (ALCL), classic pattern. This case comprises cohesive sheets of large pleomorphic cells (A, hematoxylin & eosin), shows diffuse strong expression of CD30 (B) and loss of several T-cell antigens including CD3 (C). Nuclear and cytoplasmic expression of ALK protein (D) is indicative of a fusion. An gene rearrangement can be confirmed by break-apart fluorescence hybridization (FISH) (E, the rearranged allele is represented by split red and green signals). The cells are positive for cytotoxic markers (F, perforin) and nuclear phospho-STAT3 (G). (H-N) ALK-negative ALCL with rearrangement. This case comprises cohesive sheets of neoplastic cells, including kidney-shaped hallmark cells (H, hematoxylin & eosin, arrows) and cells with doughnut-shaped nuclei (H, arrowheads). The cells are strongly CD30-positive (I), weakly positive for CD3 (J), and ALK-negative (K). Break-apart FISH shows a locus rearrangement (L, the rearranged allele is represented by split red and green signals). The cells are negative for cytotoxic markers (M, T-cell intracellular antigen 1 [TIA-1]) and phospho-STAT3 (N).",yes
PMC4520978,Figure_6,oa_package/94/ba/PMC4520978.tar.gz,"['org/1999/xlink"" xlink:href=""10-1055-s-0035-1547367-i140071-5""/>\nAn axial, T2 view.']","Fig. 6 An axial, T2 view. The mass measured to be 2.541 cm.",yes
PMC8885231,Figure_1,oa_package/60/19/PMC8885231.tar.gz,[],Image 1 A hypoechoic wedge-shaped area (arrow) seen on ultrasound in the righttesticle on sagittal view.,yes
PMC9330474,Figure_2,oa_package/84/76/PMC9330474.tar.gz,"['Unilaterally human alpha-synuclein expression in the right SN led to poor motor performance in USP+/+ (WT), compared to USP+/ or USP13 / mice (A, n = 7 12 per group) via Rotarod.', 'Interestingly, BK50118-C treatment led to a significant motor improvement in USP13+/+ (B, n = 6 12) compared to DMSO treated mice, but these effects were not observed in either USP+/ (C, n = 3 8) or USP13 / (D, n = 3 7) mice.', 'Rotarod behavior test in mice.']","Figure 2 Rotarod behavior test in mice. Rotarod test showed that lentiviral expression of human alpha-synuclein induced motor and behavioral symptoms in wild-type (USP ) but not in partially (USP ) or completely (USP13 ) mice ( ). BK50118-C improves motor and behavioral symptoms in wild-type USP ( ), but not in USP ( ), or USP13 ( ) mice. The asterisk indicates statistically significant difference. One-way ANOVA. * < 0.05. = 312. Mean SD. The black dot represents each individual data. ns: no significance.",yes
PMC8967073,Figure_3,oa_package/8a/38/PMC8967073.tar.gz,"['Pyloric stricture that has been crossed by the wire, and the wire was placed in the stomach and was dilated via a balloon.']","Figure 3 Pyloric stricture that has been crossed by the wire, and the wire was placed in the stomach and was dilated via a balloon. Imaging modality: Fluoroscopy. The arrow indicates the balloon used to dilate the stricture prior to stent placement.",yes
PMC10696332,Figure_4,oa_package/92/8e/PMC10696332.tar.gz,"['[25] [] showed an axial T2W MRI image at the level of L4 vertebra in one of the subjects with facet joint arthrosis.', ':Axial T2-weighted magnetic resonance imaging at the level of L4 vertebrae shows narrowing of bilateral facet joint spaces, marginal osteophytes, and joint hypertrophy (red arrows).']","Figure 4: Axial T2-weighted magnetic resonance imaging at the level of L4 vertebrae shows narrowing of bilateral facet joint spaces, marginal osteophytes, and joint hypertrophy (red arrows). There is also severe spinal canal stenosis.",yes
PMC6055106,Figure_2,oa_package/8b/6c/PMC6055106.tar.gz,"['1177_2050313X18787646-fig2""/>Since the patient presented a high surgical risk (Euroscore II 7.']",Figure 2. Contrast-enhanced computed tomography sagittal oblique reconstruction demonstrating the right upper pulmonary vein (arrow) draining to the superior vena cava (asterisk).,yes
PMC11483584,Figure_5,oa_package/dc/47/PMC11483584.tar.gz,"['AT8-positive cell processes in the CA1 region and cellular inclusions appeared at 12 months of age and then increased with age (A, D).', 'The same individuals showed an age-dependent increase of IBA1-positive microglia and GFAP-positive astrocyte from 7 months of age compared with age-matched control mice (B, C, E and F).', 'While there was noticeable individual variability in both IBA1 and GFAP signals at 15 18 months of age was evident, statistical analysis demonstrated a significant difference between rTKhomo and control mice in the IBA1 signal at 15 and 18 months of age, and in the GFAP signal at 18 months of age (E, F).', 'There were significant correlations in all comparisons, suggesting evidence of tau pathology-dependent glial activation (G, H).', 'Age-dependent glial phenotypic changes in hippocampi of rTKhomo and control mice.', 'These morphological differences are likely due to the presence or absence of tau pathology (A).', 'In association with the progression of AT8-positive tau distribution, IBA1-positive microglial proliferation was observed in the same hippocampal subregions (B and E).', 'In association with pathological tau accumulation, signals of glial markers, IBA1 and GFAP were increased from 12 months of age ().']","Figure 4 ( ) [ F]PM-PBB3 the standardized uptake value ratio (SUVR) images (cerebellar signal as reference) in 18-month-old rTKhomo (Tau+/+, tTA+/) and control (Tau+/+, tTA/) mice. These images were generated by averaging dynamic data 1460min after tracer injection and superimposed on individual MRI T2-weighted images. L, left; R, right; V, ventral; D, dorsal; CR, cranial; CD, caudal. Arrow and arrow heads were signals from non-specific accumulation of [ F]PM-PBB3 to submandibular gland (white arrow) and Harderian gland (white arrowheads). ( ) Time courses of SUVR in hippocampus of rTKhomo and control mice. Data are presented as mean SEM. ( ) Comparisons of regional SUVR values 4560min after tracer injection between two genotypes (rTKhomo, = 3; control, = 3). Values are mean SEM. Unpaired test was performed (* < 0.05). ( ) Forebrain right hemispheres were dissected from 3, 7, 12, 15 and 18-month-old mice for western blotting and weighed. There was no significant difference between the two groups. ( ) Volumetric analysis of hippocampus of 18-month-old rTKhomo and control mice by MRI T2-weighted imaging (rTKhomo, = 6; control, = 6). Values are mean SEM. Unpaired test was performed (* < 0.05). ( ) NeuN immunoreactivities in hippocampus from 18-month-old rTKhomo and 1518-month-old control mice. Arrowheads indicate thinner CA1 pyramidal cell layer observed in rTKhomo mice. Dotted lines indicate pyramidal cell layers of CA1, CA2, CA3 and dentate granular cell layer (DG). Scale bars = 200m. ( ) Thicknesses of CA1 ( ) and CA3 ( ) pyramidal cell layers and DG ( ) in 18-month-old rTKhomo (male, = 1; female, = 6) and 1518-month-old control (male, = 4; female, = 1) mice. Thickness values are mean SEM. Unpaired test was performed (** < 0.01, * < 0.05).",yes
PMC10657786,Figure_4,oa_package/76/7b/PMC10657786.tar.gz,"['Sagittal T2 images demonstrate the artifacts over the scalp, which at places appear to be gently scalloping the cortical outlines of the skull.']","Figure 4 Sagittal T2 images demonstrate the artifacts over the scalp, which at places appear to be gently scalloping the cortical outlines of the skull.",yes
PMC10357655,Figure_4,oa_package/2e/07/PMC10357655.tar.gz,"[') After completion of the external beam radiation therapy, he underwent two HDR interstitial brachytherapy treatments to a total dose of 19 Gy in two fractions (figure 4).', 'Brachytherapy treatment plan.']",Figure 4 Brachytherapy treatment plan.,yes
PMC7988316,Figure_1,oa_package/16/d0/PMC7988316.tar.gz,"['The inclusion criteria were: (1) prior Fontan operation using TCPC technique (lateral tunnel Fontan connection and extracardiac conduit connection); (2) age 15 years; (3) documented atrial arrhythmia excluding atrial arrhythmias occurring within the first 12 months after TCPC; (4) duration of follow-up 12 months.', 'Schematics showing atriopulmonary Fontan (A), Lateral tunnel/intra-atrial conduit Fontan (B), and Total cavopulmonary Fontan connection (C).']","Fig. 1 Schematics showing atriopulmonary Fontan (A), Lateral tunnel/intra-atrial conduit Fontan (B), and Total cavopulmonary Fontan connection (C).",yes
PMC11472455,Figure_3,oa_package/95/02/PMC11472455.tar.gz,"['Most sports combine isotonic and isometric components, which led to a new categorization into skill, power, mixed, and endurance sports103 ().', 'Differentiation of sports in relation to the predominant isotonic and isometric component, intensity, and frequency (adapted from the 2020 ESC guidelines on sport cardiology and exercise in patients with cardiovascular disease103)Healthy children typically experience varying blood pressure levels during treadmill, with systolic blood pressure increases 160 mmHg in boys after age 14 while usually remains below 160 mmHg in girls regardless of age.']","Figure 2 Echocardiographic imaging of the aorta at different levels. Parasternal long-axis view of the aortic annulus, sinus of Valsalva, and sinotubular junction measured in systole using the inner-to-inner method. Measurements should be taken perpendicular to blood flow. Parasternal long-axis view of the sinus of Valsalva and sinotubular junction measured in diastole using the leading edge-to-leading edge method. Parasternal short-axis view of the aortic valve measured in diastole and using the largest aortic diameter. Parasternal long-axis of the ascending aorta, measured in systole at the level of the right pulmonary artery, using the inner-to-inner method. Suprasternal view of the aortic arch measured in systole between the truncus brachiocephalicus and the left carotid artery, using the inner-to-inner diameter. Modified apical two-chamber view of the descending aorta measured at the level of the left atrium in systole using the inner-to-inner method. Subcostal view of the abdominal aorta at the level of the liver, measured in systole using the inner-to-inner diameter. Ao, aorta; AP2CH, apical two-chamber view; LA, left atrium; LV, left ventricle; PSLAX, parasternal long-axis view; PSSAX, parastenal short-axis view",yes
PMC11422228,Figure_1,oa_package/04/dc/PMC11422228.tar.gz,[],"Figure1 The schematic diagram illustrating the structure and distribution of blood vessels and lymphatic vessels in bones and joints. In bones, two distinct types of blood vessels are identified: type H and type L. Type H vessels, characterized by high expression of endomucin (Emcn) and cluster of differentiation 31 (CD31), are organized in a columnar manner with arterial connections and are primarily found in the metaphysis. Conversely, type L vessels, with lower levels of Emcn and CD31, are sinusoidal and located in the diaphysis. The identification of these vascular subtypes enhances our understanding of the heterogeneity of bone vasculature and its potential role in bone function in both health and disease. The lymphatic system in bones and joints also displays a hierarchical structure. Lymphatic vessels are present in the cortical regions and bone marrow cavity, with a higher concentration in the cortical areas. In joints, the lymphatic system begins with lymphatic capillaries, also known as initial lymphatic vessels. These vessels collect lymph and direct it towards collecting lymphatics equipped with anti-flowback valves. The lymph is then transported to draining lymph nodes before entering the venous system. Initial lymphatic vessels consist of a single layer of lymphatic endothelial cells (LECs) with a discontinuous basal lamina. In contrast, collecting lymphatic vessels are composed of tightly connected LECs, forming zipper-like junctions, and are surrounded by lymphatic muscle cells (LMCs) that facilitate lymph movement through contractions. Initial lymphatic vessels are marked by positive expression of lymphatic vessel endothelial hyaluronan receptor 1 (LYVE1), podoplanin (PDPN), prospero homeobox 1 (PROX1), and vascular endothelial growth factor receptor 3 (VEGFR3), but do not contain -smooth muscle actin (-SMA)-positive muscle cells. Collecting lymphatic vessels, however, exhibit lower levels of LYVE1 and positive expression of -SMA, PDPN, PROX1, and VEGFR3. This differentiation between initial and collecting lymphatic vessels highlights their distinct roles and structures within the lymphatic system of bones and joints. (This figure is supported by ).",yes
PMC8670334,Figure_3,oa_package/80/a6/PMC8670334.tar.gz,['Pathological images.'],Fig. 3 Pathological images. (a) Hematoxylin and eosin (HE) stains of the lymph nodes with viable tumor cells before neoadjuvant therapy. (b) HE stains of the lymph nodes with highly degenerative tumor cells after neoadjuvant therapy. (c) HE stains of the primary tumor bed with no surviving tumor cells after neoadjuvant therapy.,yes
PMC7690460,Figure_2,oa_package/44/9c/PMC7690460.tar.gz,['Ultrasound (USG) B-scan revealed ITQ choroidal mass with high surface reflectivity and low to medium internal reflectivity with no acoustic hollowing or choroidal excavation [].'],"Figure 2 Ultrasound B scan of right eye at presentation, showing a well-circumscribed subretinal mass in the ITQ with circumferential basal diameter-13.5 mm, height-6.9 mm, anteroposterior basal-11.7 mm, associated shallow retinal detachment with shifting fluid. Features of acoustic hollowing, choroidal excavation, sound attenuation or orbital mass lesions were absent",yes
PMC7653184,Figure_17,oa_package/0e/04/PMC7653184.tar.gz,[],"Figure 17 A and B: Axial and sagittal computed tomography (CT) images showing long-segment esophageal stenosis related to caustic ingestion (orange arrows); C and D: With an initial upper endoscopy aborted, repeat upper endoscopy with the addition of fluoroscopic guidance allowed passage through a known stricture and eventual stent placement; E: Subsequent fluoroscopic upper gastrointestinal examination showing free contrast passage through the stented esophagus, with direct sagittal comparison to the initial CT in the sagittal plane.",yes
PMC9708566,Figure_12,oa_package/20/b1/PMC9708566.tar.gz,[],"Extended Data Fig. 5 Murine bassoon downregulation does not produce gross brain abnormalities. , Western blot and quantification of BSN downregulation in WT mice. , Detection of BFP2 reporter in AAV scramble and AAV shBSN mice, confirming the widespread expression of both sequences. , H&E staining of WT mice injected with a scramble and shBSN shRNA. , BSN and Syn-1 immunofluorescence in WT mouse cortexes injected scramble and shBSN shRNA, and mean intensity of BSN and Syn-1. Data are shown as the mean s.e.m. Experiments were performed with =8 ( ). Significance was determined by unpaired two-tailed Students t-test ( ), and one-way ANOVA ( ).",yes
PMC6180189,Figure_1,oa_package/58/77/PMC6180189.tar.gz,['Shear stress modifier induces rapid atherosclerosis in mice.'],"Figure 1 Shear stress modifier induces rapid atherosclerosis in mice. Diagram of the shear stress modifier. The cuff imposes a gradual stenosis from 600 m to 300 m of inner diameter at downstream end with a cone shaped lumen. BSSMs on the common carotid arteries. T, trachea; thick arrow, shear stress modifier. Gross specimen of main arteries 8 weeks after bilateral placement of the shear stress modifier on high calorie diet. The opaque white regions of the vessels correspond to the sites of extensive atherosclerotic plaque deposition both upstream and downstream (thin arrow indicating the blood flow direction) of the cuff (thick arrow, ), better viewed after oil red O staining . No significant difference of the serum concentration levels of low-density lipoprotein (LDL), triglyceride (TG) and cholesterol (CHO) among sham ( = 12), USSM ( = 11) and BSSM ( = 9) groups. Sham: sham surgery group; Unilateral: unilateral implantation of shear stress modifier (USSM); Bilateral: bilateral implantation of shear stress modifier group (BSSM).",yes
PMC9569960,Figure_6,oa_package/5f/c3/PMC9569960.tar.gz,"['4 108 per mL), suggesting the presence of GSK3 facilitated the packaging of tau into EVs (A).', 'To confirm the identity of exosomes, isolated samples were analyzed by Western blot analysis using anti-Syntenin-1 (B), which is a marker of the syndecan syntenin alix-dependent pathway of exosome biogenesis [35].', 'Lastly, the presence of tau packed into the exosomes was confirmed by Western blotting using Tau-5 antibody for protein samples extracted from exosomes (C).', 'The uptake of fluorescently labeled exosomes by differentiated SH-SY5Y cells was analyzed by a fluorescence microscope after 24 h (D).', 'Interestingly, SH-SY5Y recipient cells treated with exosomal tau phosphorylated by GSK3 had increased exosomal tau uptake, as well as higher amounts of tau aggregates (D), which suggests a higher seeding potency of phosphorylated tau than wild type tau-containing exosomes.', 'Total tau protein present in the cell lysates of SH-SY5Y neurons treated with GSK3 -phosphorylated tau-containing exosomes was much higher than that in cell lysates from those treated with wild type 0N4R-containing exosomes (E).', 'Further, we established an in vitro tau transcellular model by treating differentiated SH-SY5Y cells with phosphorylated tau-containing exosomes ().', 'Additionally, the transmissivity and toxicity of tau species was greatly enhanced by phosphorylation, as evident by a marked increase in tau aggregates formed in SH-SY5Y cells treated with exosomal tau pre-phosphorylated by GSK3 ().', 'Tau inter-neuronal propagation is promoted by phosphorylation.']","Figure 6 Tau inter-neuronal propagation is promoted by phosphorylation. Characterization of EVs isolated from HEK293TN cells transfected with the XPack-0N4R construct (0N4R) without or with GSK3 (GSK3 + 0N4R) co-transfection. ( ) ZetaView was used for measuring particle concentration (10 particles/mL). The presence of exosomes was confirmed by immunoblotting isolated exosome lysates against the exosome biosynthesis marker, Syntenin-1 with a molecular weight of 34KDa ( ). Immunoblotting exosome lysates against tau specific antibody Tau-5 ( ) confirmed that tau protein was packaged into exosomes. ( ) Mature neurons differentiated from SH-SY5Y cells were imaged under a fluorescent microscope after being treated with SYTO RNASelect Green Fluorescent Cell Stain (ThermoFisher, Waltham, MA, USA) labeled exosomes for 24 h at 37 C. The scale bar denotes 100 m. ( ) Total human tau ELISA of recipient cell lysates shows more tau was taken up from EVs isolated from GSK3 co-transfection (* represents < 0.05 between two different groups, = 2).",yes
PMC2194026,Figure_4,oa_package/8c/26/PMC2194026.tar.gz,"['As can be seen in Figs.', '.']","Figure 4. Number of R1Rv in the lungs, livers, and spleens of WT, TCR- , and IFN- mice at day 50 of an aerogenic initiated with 10 CFU. Infection was exacerbated substantially in all organs of the mutant mice. Means of five mice SD.",yes
PMC5968116,Figure_5,oa_package/50/16/PMC5968116.tar.gz,['Distribution of cFos immunoreactive cells in the caudal PAG.'],"Figure 5 Distribution of cFos immunoreactive cells in the caudal PAG. Coronal diagram of the rat brain at corresponding Bregma level AP 8.00 mm (Rat brain atlas, Paxinos and Watson, ). Representative low power photomicrographs of coronal brain sections stained for cFos showing the PAG of non-stimulated (Sham) rat and stimulated rat (Stim) with bipolar stimulation electrode implanted in the bladder wall at the dome and neck levels. The higher power photomicrographs in the lower right corners focus on the central region of the vlPAG column. Aq, aqueduct; DRN, dorsal raphe nucleus; dlPAG, dorsolateral PAG; dmPAG, dorsomedial PAG; lPAG, lateral PAG; vlPAG, ventrolateral PAG. Scale bar = 100 m.",yes
PMC9247196,Figure_3,oa_package/08/21/PMC9247196.tar.gz,[],"Figure3 45-year-old woman with thyroid papillary carcinoma. Sonographies of longitudinal scanning and axial scanning show the nodule locates at the left lobe of the thyroid, characterized by 28.9mm19.7mm19mm in size, mixed solid-cystic composition, irregular shape, ATh2 response is not restored by transfer of normal dendritic cells into 5i KO miceTo study whether Th2 driven responses can be rescued in 5i KO mice, we attempted to restore iDCs deficiencies of 5i KO mice by adoptive transfers of in vitro generated Wt BMDCs (A).', 's004""> S4A), Wt but not 5i KO mice showed increased allergic inflammation upon OVA-challenge (B, C).', 'Nevertheless, transfer of BMDCs into 5i KO mice increased BAL lymphocytes and completely restored BAL neutrophils numbers to Wt levels (B), which corresponds with the increased frequency of lung Th1 cells (Differential effects of TP isoforms on trophoblast cell cycle.']","Figure 2 Differential effects of TP isoforms on trophoblast cell cycle. ( ) Proliferation of BeWo cells () transfected with empty vector- ( ), TP (O), TP () and TP () was monitored over 168hours. ( ) Proliferation of JEG-3 cells expressing similar constructs, stimulated with vehicle () or 100nM I-BOP () was documented over the same period. ( ) Apoptosis of BeWo cells under basal () and serum-deprivation () was assessed after 24hours using flow cytometry. ( ) Distribution of transfected BeWo lines in phases of the cell cycle (G0/G1 , S , G2/M ) was assessed also using flow cytometry. The data are represented as meanSEM and are representative of 3 independent experiments. One-way ANOVA and Independent samples T-test, * <0.05, ** <0.01, *** <0.005.",yes
PMC8366622,Figure_3,oa_package/9e/e6/PMC8366622.tar.gz,"['We used the same two-shot regimen as shown above for immunologic evaluation of several other adjuvants, alone or in combination, for us with K562-S-FI ((A)).', 'As such, all the animals in Alum or MnJ plus CpG group displayed a high neutralizing antibody titer of 2000 ((B,C), Supplementary A).', 'The results showed that Alum or MnJ adjuvant drove the K562-S-FI-induced immunity toward a Th2-biased phenotype whereas their combination with CpG reversed Th2 biasing and induced a balanced T cell response ((D,E)).', '.']","Figure 3. Identification of optimal adjuvant for K562-S vaccine in induction of humoral immunity against SARS-CoV-2. (A) Experimental schedule. Female C57/BL/6 ( =6/group) mice were subjected to a homologous prime-boost regimen with 4-week interval consisting of K562-S-FI either non-adjuvanted or adjuvanted with Alum, MnJ, CpG, Alum plus CpG, or MnJ plus CpG. (B and C) Serum antibody responses were assessed at week 6 post prime by RBD-specific binding antibody ELISA (B) and pseudovirus neutralization assays (C). (D and E) Serum levels of SARS-CoV-2 RBD-specific IgG1 and IgG2c antibodies as determined by an ELISA (D) and the derived IgG2c/IgG1 ratios (E). Antibody titer data were presented as geometric mean titers (GMT)geometric standard deviation (GSD). KruskalWallis test with Dunns adjustment were applied when comparing groups. * <0.05, ** <0.01, *** <0.001; **** <0.0001, ns=non-significant.",yes
PMC3542774,Figure_4,oa_package/7e/d7/PMC3542774.tar.gz,"[""L'ut rus et les ovaires sont d'aspect normal avec panchement intra p riton al de faible abondance ()."", 'IRM pelvienne: hydrosalpinx bilat ral contenu en hyposignal T2 et parois rehauss e apr s contrasteObservation 4Mlle I.']",Figure 4 IRM pelvienne: hydrosalpinx bilatral contenu en hyposignal T2 et parois rehausse aprs contraste,yes
PMC6761706,Figure_9,oa_package/26/1d/PMC6761706.tar.gz,['.'],"Figure 9. Histopathology; rash appears to be a psoriasiform dermatitis, likely secondary to drugs.",yes
PMC8938594,Figure_4,oa_package/4f/aa/PMC8938594.tar.gz,"['05 illustrates PE diagnosed by AI and not radiologists, and PE diagnosed by a radiologist and not AI.', 'Clinical examples.', 'Two segmental PEs were correctly diagnosed by the emergency radiologist but missed by the AI algorithm (white arrows)Clinical use of AI by radiologists A, B shows the results of the satisfaction survey.']","Figure 4 Clinical examples. A 71-year-old patient with a medical history of cancer and recent surgery presented with heart rate > 95 beats per minute and a borderline saturation and underwent a contrast-enhanced CT pulmonary angiogram (CTPA) ( ), which showed a segmental, sub-acute, pulmonary embolism (PE) in the right low limb, which was missed by the emergency radiologist during his on-call duty (red arrow). On the same cross-section, the PE was correctly identified by the artificial intelligence (AI) algorithm (AIDOC Medical). Example of pulmonary embolism (PE) correctly diagnosed by the emergency radiologist and not by the artificial intelligence (AI) algorithm. Opposite example: An 85-year-old patient with a medical history of PE and a recent surgery presented with a heart rate between 75 and 94 beats per minute and acute dyspnea and underwent CTPA ( ). Two segmental PEs were correctly diagnosed by the emergency radiologist but missed by the AI algorithm (white arrows)",yes
PMC10445368,Figure_3,oa_package/d5/1b/PMC10445368.tar.gz,"['Transthoracic and transoesophageal echocardiography revealed multiple tiny vegetations at the septal leaflet of the tricuspid valve on the atrial side (figure 3) with near moderate tricuspid regurgitation with a peak gradient of 15 mm Hg across the tricuspid valve.', 'Transoesophageal echocardiography revealed multiple tiny vegetations at the septal leaflet of tricuspid valve on the atrial side.']",Figure 3 Transoesophageal echocardiography revealed multiple tiny vegetations at the septal leaflet of tricuspid valve on the atrial side.,yes
PMC5551918,Figure_5,oa_package/e6/2b/PMC5551918.tar.gz,"['49, consistent with known MPNST (', '']","Fig.5 The axial nonattenuated corrected (A), coronal attenuated corrected (B), axial fused positron emission tomography-computed tomography (PET-CT) (C), and sagittal fused PET-CT (D) images demonstrated a very hypermetabolic mass involving the right brachial plexus.",yes
PMC7700800,Figure_1,oa_package/96/65/PMC7700800.tar.gz,"['Initial CT imaging findings demonstrated severe, heterogenous right-sided bullous emphysematous changes with a basal predominance and multiple large bullae in the lower lobe () and a benign inflammatory nodule, which was 7 mm in size in the right upper zone presumably right upper lobe.', 'CT scan demonstrating heterogeneous bullous emphysema with hyperinflation of the right lung and mediastinal shift.']",Figure 1 CT scan demonstrating heterogeneous bullous emphysema with hyperinflation of the right lung and mediastinal shift.,yes
PMC7977697,Figure_42,oa_package/de/90/PMC7977697.tar.gz,[],Figure 17b: Acute exacerbation of idiopathic pulmonary fibrosis. Zoomed view of axial CT demonstrates a probable usual interstitial pneumonia pattern in a patient with idiopathic pulmonary fibrosis. Zoomed view of axial CT obtained after rapid deterioration in symptoms demonstrates CT findings of acute exacerbation with progression of reticular abnormality and marked new superimposed ground-glass opacity. Other causes of airspace disease including infection were clinically excluded. Zoomed view of axial CT obtained after resolution of acute exacerbation confirms that the background fibrosis has substantially progressed.,yes
PMC4158089,Figure_3,oa_package/95/d1/PMC4158089.tar.gz,"['Astrocyte-derived galectin-9 promotes microglial TNF secretionTo determine if galectin-9 was responsible for the synergic response from astrocytes, we stimulated control (Lgals9+/+ or Lgals9+/ ) and Lgals9 / mixed glial cultures with poly(I:C) and measured TNF secretion.', 'However, mixed glia derived from Lgals9 / mice produced approximately 35% less TNF upon activation than control cultures ( 3A).', 'The number of microglial cells in these cultures did not differ between genotypes ( 3B), thereby excluding the possibility that the decrease in TNF production was attributable to culture variation.', 'However, primary microglia isolated from Lgals9+/+ and Lgals9 / mice produced similar amounts of TNF after poly(I:C) stimulation ( 3C).', 'Likewise, production of TNF in enriched astrocyte cultures, which is attributable to residual contaminating microglia [43] was similar between genotypes ( 3C).', 'Stimulation of microglia co-cultured with Lgals9+/+ astrocytes produced more TNF than either microglia alone or microglia co-cultured with Lgals9 / astrocytes, demonstrating that astrocyte-derived galectin-9 promotes TNF production by microglia ( 3D).', 'As such, we questioned if the effects of galectin-9 on microglia TNF production in mixed glial cultures ( 3A) were mediated by Tim-3 signaling.']","Figure 3 Mono-, co- or mixed glial cultures obtained from littermate control ( or ) or mice were stimulated with poly(I:C) (25g/ml) for 24hours and TNF measured from the supernatants by ELISA. Reduction in supernatant levels of TNF obtained from mixed glia despite similar numbers of Iba-1 microglia across genotypes . Results in are combined meansSE of 5 independent experiments ( =19; =12); results in are combined meansSE of glial cultures depicted in chosen at random ( =3; =3). Scale bar, 50m. TNF levels from poly(I:C) activated astrocyte and microglial monocultures derived from or mice. Results are meansSE of quadruplicate samples and are representative of two independent experiments. Supernatant levels of TNF following poly(I:C) stimulation of microglial monocultures compared to co-cultures containing (wild-type (WT); ) or (knockout (KO); ) astrocytes. Results are meansSE of quadruplicate samples and are representative of four independent experiments. * <0.05, ** <0.01, *** <0.001.",yes
PMC5061302,Figure_2,oa_package/57/19/PMC5061302.tar.gz,[],Fig. 2 Large chest wall sarcoma measuring 27cm16cm16cm demonstrated (a) during and (b) after excision.,yes
PMC10503188,Figure_2,oa_package/c2/fb/PMC10503188.tar.gz,['The arrow is pointing at the portal vein on (Computerised tomography scan with intravenous contrast)MSCT showing right aberrant hepatic artery (red arrow) communicating with vascular sac (Astrix) with communication to left portal vein (blue arrow) representing a complex intrahepatic arterioportal malformation that is no direct communication'],Fig. 2 Portal phase showing early opacification of portal vein. The arrow is pointing at the portal vein on (Computerised tomography scan with intravenous contrast),yes
PMC3765121,Figure_1,oa_package/8c/3b/PMC3765121.tar.gz,['Gadolinium-enhanced axial T1-weighted brain magnetic resonance imaging scans showing three metastatic cystic brain lesions in the right parieto-occipital and temporal regions with associated perifocal edema.'],Figure 1 Gadolinium-enhanced axial T1-weighted brain magnetic resonance imaging scans showing three metastatic cystic brain lesions in the right parieto-occipital and temporal regions with associated perifocal edema.,yes
PMC3302790,Figure_7,oa_package/cb/a0/PMC3302790.tar.gz,"['Here we provide evidence that both, JNK1 and JNK2 can be seen in Bim positive protein complexes, but it is JNK2 only that is increased in this protein pool from whole cell lysates ().', 'g007JNK2-Bim co-precipitation.']",10.1371/journal.pone.0030985.g007,yes
PMC8389213,Figure_4,oa_package/85/8f/PMC8389213.tar.gz,"['A significant transcriptional downregulation of cat-D was observed in NaIO3-treated cells relative to controls (A).', 'Subsequent evaluation of two independent samples by WB revealed significantly reduced levels of pro-cat-D, and additionally, decreased maturation of pro-cat-D to its mature form (B, lanes 2 and 4 vs.', '1 and 3; C).', 'The ratio of the intensities clearly indicated a diminished maturation of cat-D from its pro form in NaIO3-treated cells relative to controls (D).', 'Successful downregulation of cat-D resulted in increased accumulation of ferritin relative to scrambled siRNA-treated controls (E, lanes 2 vs.', '1; F), suggesting ferritin to be one of the substrates processed by cat-D in these cells.', 'Synthesis and maturation of cathepsin-D is compromised by NaIO3.']","Figure 4 Synthesis and maturation of cathepsin-D is compromised by NaIO ( ) Quantitative RT-qPCR for cat-D showed 0.75-fold downregulation in lysates from NaIO -treated ARPE-19 cells relative to controls. Human GAPDH was amplified in parallel. ( ) Probing of NaIO -treated cell lysates for cat-D showed decreased expression of pro-cat-D and its mature form relative to controls (lanes 2, 4 vs. 1, 3). ( ) Densitometry after normalization with -actin showed 0.7- and 0.5-fold decreases in the levels of pro-cat-D and mature cat-D in NaIO -treated samples relative to the controls. ( ) The densitometry ratio of mature cat-D relative to pro-cat-D was significantly lower in NaIO -treated cell lysates relative to control. ( ) Probing of ARPE-19 cell lysates for cat-D showed the expected reactivity of the pro-cat-D form at 52 kDa in control cells transfected with scrambled siRNA, and minimal reaction in cells exposed to cat-D siRNA (lane 2 vs. 1). This further led to lowered levels of mature cat-D levels in cat-D siRNA-treated cell lysates. Probing for ferritin showed significant upregulation in the absence of cat-D relative to control (lanes 2 vs. 1). ( ) Quantification by densitometry after normalization with -actin showed 2.7-fold upregulation of ferritin due to downregulation of cat-D. Full blots and their details are provided in . Values are means SEM of the indicated . * 0.05; ** 0.01; *** 0.001.",yes
PMC5989536,Figure_4,oa_package/47/80/PMC5989536.tar.gz,"[' presents zoomed screenshots of Helicobacteria in a gastric biopsy scanned with resolutions of 0.', 'Digitally magnified screenshots of whole-slide images 14 (a, c, e) and 16 (b, d, f)Effects of image compression and whole-slide image scanning resolution on detecting Helicobacteria in a gastric biopsy.']",Figure 4 Effects of image compression and whole-slide image scanning resolution on detecting Helicobacteria in a gastric biopsy. JP2-WSI (a and c) and JPEG quality level 80 (b and d) compressed images scanned at 0.31 m/pixel (a and b) and 0.16 m/pixel (c and d) sampling resolutions,yes
PMC9816291,Figure_1,oa_package/a8/0c/PMC9816291.tar.gz,"[' 1).', 'Phosphorylated tau neuropathology in the dorsolateral frontal cortex and hippocampus of six deceased American football players.', 'The third column depicts the posterior hippocampus (C,F,I,L,O,R) (scale bar = 3 mm)The two non-CTE cases (cases 1 and 2; both former college players) had the lowest overall burden of p-tau.']","Fig. 1 Phosphorylated tau neuropathology in the dorsolateral frontal cortex and hippocampus of six deceased American football players. Representative images of hyperphosphorylated tau (AT8 antibody) staining from former American football players. Of the six cases, two individuals did not receive a diagnosis of CTE (cases 1 and 2), three had CTE stage III (cases 35), and one had CTE stage IV (case 6). Of note, case 4 had low cortical tau burden (i.e., cortical sparring) but had high burden in the medial temporal lobes. The first column depicts a low power overview of cortical regions (A,D,G,J,M,P) (scale bar=3mm). All cortical images came from the dorsolateral frontal cortex except case 4, which came from the entorhinal cortex given it was a low cortical burden case of CTE. The second column shows a high-power view of perivascular tau pathology (B,E,H,K,N,Q) (scale bar=200m). No perivascular tau was observed in cases 1 and 2. The third column depicts the posterior hippocampus (C,F,I,L,O,R) (scale bar=3mm)",yes
PMC6548166,Figure_4,oa_package/d0/d3/PMC6548166.tar.gz,['(C) 8 slice images on the coronal section by 3D SWE of a thyroid papillary carcinomaA papillary carcinoma missed by B-mode US (TI-RADS 4a) but suspicious by 2D SWE (S-Tmean=25.'],Figure 4 A papillary carcinoma missed by B-mode US (TI-RADS 4a) but suspicious by 2D SWE (S-Tmean=25.3 kPa).,yes
PMC8539093,Figure_11,oa_package/a5/5f/PMC8539093.tar.gz,[],"Figure 11 MassReg registration results after experiment 2 of training on real un-registered image pair. Left to right: DESI-MS images, corresponding pathology masks, registered DESI images with Elastix, registered DESI images with MassReg, transformation field from MassReg output.",yes
PMC10688981,Figure_2,oa_package/a5/43/PMC10688981.tar.gz,"['Next, we performed bulk RNA-Seq of pemphigus skin samples with TLSs (n = 5) and without TLSs (n = 5) and compared their gene signatures (A).', 'Consistent with the phenotypes, gene set enrichment analyses (GSEAs) showed that TLS-related gene signatures were upregulated in pemphigus skin lesions with TLSs compared with those without TLSs (Supplemental A).', 'Gene ontology (GO) term analysis revealed that chemokine activity and chemokine receptor binding genes were upregulated in skin lesions with TLSs compared with those without TLSs (B and Supplemental Table 2).', 'We identified 14 differentially expressed genes (DEGs) belonging to chemokine and chemokine receptor gene sets (C, Supplemental B, and Supplemental Table 3).', 'In immunofluorescence studies, CXCL13+ cells were mostly located in TLSs (D).', 'In GSEA, Th1, Th17, and Tfh cell gene signatures were enriched in skin lesions with TLSs, but the Th2 cell gene signature was not (G).', 'The top 10 clones constituted more than 50% of the total TCR repertoire (H).', 'CD4+ T cells are the major producer of CXCL13 in skin TLSs in pemphigus.']","Figure 2 CD4 T cells are the major producer of CXCL13 in skin TLSs in pemphigus. ( ) Bulk RNA-Seq of tertiary lymphoid structurepositive (TLS-positive) and negative samples ( = 5 each). ( ) Gene ontology (GO) analysis and ( ) volcano plot depicting upregulated (red dots) and downregulated DEGs (blue dots). ( ) Representative immunofluorescence staining for CXCL13 (green) and CD20 (red) in skin TLSs from a patient with pemphigus. Nuclei were stained with DAPI (light gray). Scale bar: 100 m. ( ) Representative immunofluorescence staining for coexpression of CD4 (red) and CXCL13 (green) in skin TLSs. White arrowheads indicate CXCL13 CD4 cells. Nuclei were stained with DAPI (light gray). Scale bar: 50 m. ( ) Percentage of CD4 , CD8 , CD20 , CD138 , FDC , and HLA-DR cells in CXCL13 cells from immunofluorescence images ( = 5). Data are shown as the mean SD. ( ) Gene set enrichment analysis of Th1, Th2, Th17, and Tfh cell gene signatures using the transcriptome of TLS-positive versus TLS-negative samples. ( ) Percentage of top 10 most frequently occurring clones of TCR chain from bulk TCR-Seq in skin TLSs from 5 patients (no. 15).",yes
PMC4976402,Figure_4,oa_package/da/bf/PMC4976402.tar.gz,"['1%), [], steroid induced rosacea [] in 28 patients (6.']",Figure 4 Perioral dermatitis with hyperpigmentation,yes
PMC11277532,Figure_3,oa_package/47/8d/PMC11277532.tar.gz,"['These data are summarized in .', 'The effects of IL-1 and IL-6 are summarized in .', 'The functions of IL-1 and IL-6 in peripheral tissues and central nervous system.']","Figure 3 The functions of IL-1 and IL-6 in peripheral tissues and central nervous system. Monocytes, IL-1, LPS, and TNF- promote the synthesis of IL6, which increase the bloodbrain barriers permeability. It stimulates the division of microglia and the differentiation of B lymphocytes. IL-6 production also leads to T lymphocytes production (and vice-versa). These pathways promote inflammation. LPS increase the level of IL-1, and thus, IL-1 activates microglia and increases the level of IL-6 via stimulating astrocytes to release this cytokine.",yes
PMC10474302,Figure_7,oa_package/80/71/PMC10474302.tar.gz,[],"Figure S4. Additional histology of muscle. Related to . COX1 (green), protein amounts, immunofluorescence (IF) analysis. Magnification 40x, scale bar 100 m. Scatterplot of SDHA mean intensity units in immunofluorescent SDHA staining in mouse skeletal muscle. SDHA (white), protein amounts, IF analysis. Magnification 20x, scale bar 200 m. TOM20 (red), protein amounts, IF analysis. Magnification 20x, scale bar 200 m. Myosin heavy chain (MHC) expression; IF staining. type IIa, (F) type IIb, (G) type IIx, and (H) type I in mouse skeletal muscle. Arrows indicate examples of fibers positive for MCH, stained in red. Images also include cell borders (laminin, in green) and nuclei (DAPI, in blue). Magnification 20x, scale bar 200 m. The images presented are representative samples of biological replicates. All graphs are mean with SD. Statistical significance determined using one-way ANOVA with -values: * ( 0.05), ** ( 0.01), *** ( 0.001), and **** ( 0.0001).",yes
PMC8597318,Figure_5,oa_package/bb/39/PMC8597318.tar.gz,"[' 5).', ' 5B), miR-526b-5p (p = 0.', ' 5G) and miR-454-3p (p = 0.', ' 5F) were found to have sexually dimorphic expression independent of fetal growth.', ' 5C) but not female, and miR-28-5p was reduced by 28% in FGR pregnancies with a female fetus (0.', ' 5A), but not when the fetus was male.', 'SD standard deviation, F female, M male, dCq = (Cq target miRNA Cq reference miRNAs), EVs extracellular vesicles, GDM gestational diabetes mellitus, PE pre-eclampsia, PTB pre-term birth, FGR fetal growth restriction, PTL pre-term labourEffect of fetal sex on microRNA levels in maternal serum in uncomplicated and FGR pregnancies.', '01Serum miRNAs correlate with hPL, a marker of placental dysfunctionTo determine whether levels of serum miRNAs altered in FGR could be indicative of placental dysfunction, the relationship between altered miRNA levels and maternal concentrations of hPL, an established marker of placental endocrine function in late pregnancy which is lower in pregnancies complicated by FGR [57, 58, 69], were assessed (']","Fig. 5 Effect of fetal sex on microRNA levels in maternal serum in uncomplicated and FGR pregnancies. qPCR was performed on individual microRNAs isolated from maternal serum of women with appropriately grown (AGA; IBC 2080th) or growth-restricted (FGR; IBC<5th) infants using specific primers for miR-28-5p, miR-29c-3p, miR-301a-3p, miR-378a-3p, miR-409-3p, miR-454-3p and miR-526b-5p. Data were normalised to 2 reference miRNAs and expressed as 2 . Data were stratified into male ( =15/group) and female ( =16/group). Individual data points shown, line represents the median. 2-way ANOVA with linear step-up multiple comparison test. Interaction (F) between the groups was considered significant when <0.05. KruskalWallis followed by Dunns post hoc analysis was used to determine difference between individual groups; * <0.05, ** <0.01",yes
PMC4230564,Figure_4,oa_package/c5/3e/PMC4230564.tar.gz,[],"FIGURE 4. Neuroradiologists. From left to right: Martin erk, Toma Kregar and Miha krbec.",yes
PMC11330160,Figure_5,oa_package/73/22/PMC11330160.tar.gz,"['The expression of Caspase 3 was evidently up-regulated in three septic groups when compared to the control group and the sham group, GTS-21 had the tendency to curb its expression, and MLA boosted its expression ().', 'Pathological study of MVZ in sepsis and intervened by CAP (TUNEL and double immunofluorescence-labeling experiments).']","Figure 5 Pathological study of MVZ in sepsis and intervened by CAP (TUNEL and double immunofluorescence-labeling experiments). shows that sepsis induces MVZ neurons (including cholinergic neurons and catecholaminergic neurons) apoptosis and inactiveness. (a) TUNEL and double immunofluorescence labeling images of MVZ. The red fluorescence in the TUNEL column indicates apoptotic cells. In the TH/Caspase 3 column, the green fluorescence indicates the expression of TH, and the red fluorescence indicates the expression of Caspase 3 in the catecholaminergic neurons. In the CHAT/Caspase 3 column, the green fluorescence indicates the expression of CHAT, and the red fluorescence shows the expression of Caspase 3 in the cholinergic neurons. From these images, we can see that sepsis not only causes significant apoptosis of catecholaminergic and cholinergic neurons in MVZ but also affects their activeness. Intervention with CAP can affect the activity and apoptosis of functional neurons in the MVZ area: 7nAChR agonists GTS-21 have the tendency to prevent the cholinergic and catecholaminergic neurons from apoptosis and inactiveness in MVZ, while 7nAChR antagonist MLA significantly accelerates apoptosis and inactiveness of these two kinds of neurons. (b) The histogram of TUNEL analysis, which confirms that sepsis-induced significant apoptosis in MVZ, activating CAP had the tendency to reverse it; on the contrary, blocking CAP exacerbated the apoptosis. (c) The histogram of TH/Caspase 3 expressions in MVZ, which suggests that sepsis causes significant apoptosis of catecholaminergic neurons in MVZ; GTS-21 can reverse the situation, whereas MLA is the opposite; in addition, both sepsis and even sham operation cause significantly low expression of TH, especially when rats were treated by MLA. (d) The histogram of CHAT/Caspase 3 expressions in MVZ, which shows that even though sepsis caused significant apoptosis of cholinergic neurons in MVZ; it did not induce the significantly reduced expression of CHAT except in the MLA group.",yes
PMC6591440,Figure_4,oa_package/81/8e/PMC6591440.tar.gz,"['When MRSA was cultured in the presence of different doses of AMSA alone, the growth of MRSA was largely unaffected up to 25 g/ml (A).', '80% inhibition of MRSA growth (B).', 'Moreover, the MIC of NaP was reduced twofold, to 125 mM, when AMSA was co-treated (C).', 'D-Alanylation inhibition increases the susceptibility of MRSA to the growth inhibition by NaP.']","Figure 4 -Alanylation inhibition increases the susceptibility of MRSA to the growth inhibition by NaP. MRSA was cultured in the presence of different doses of AMSA, a -alanylation inhibitor. Optical density was measured hourly. MRSA USA300 was cultured in the presence of 10 g/ml AMSA, 50 mM NaP, or both. Optical density was measured hourly. The MIC/MBC test was conducted using the microdilution method with NaP in the presence of 10 g/ml AMSA. The MIC, the concentration of NaP which completely inhibited growth, is indicated with an arrow. Data shown are the mean values SD of triplicate samples and are representative of at least three independent experiments.",yes
PMC11059464,Figure_4,oa_package/4a/eb/PMC11059464.tar.gz,"['Structurally, there was an increase in the soft components and a reduction in semi-rigid components ().', '.']","Figure 4. The elastosonographic analysis of the forehead shows that, although the skin structure is the same, individuals of different ages have different rigid (red), semi-rigid (green) and soft (blue) components. The distribution of these components is related to the degree of aging. On the front, the variation in the average percentage level of the semi-rigid components is dominant. Here is the elastography data of the two subjects in . ) For the 31-year-old subject, the percentage of pixels in the selection box (out of a total of 255 pixels) corresponding to red (soft) components was 32.3%, that corresponding to the green (semi-rigid) components was 44.6%, and that corresponding to the blue (hard) components was 23.4%. ) For the 65-year-old subject, the percentage of pixels in the selection box (out of a total of 255 pixels) corresponding to the red (soft) components was 37.2%, that corresponding to the green (semi-rigid) components was 29.5%, and that corresponding to the blue (hard) components was 21.2%.",yes
PMC5604944,Figure_6,oa_package/52/fe/PMC5604944.tar.gz,"['To examine the effects of fluoxetine on articular chondrocytes in vivo, rats were intraarticularly injected with 50 l of 50 M (), 100 M (S6 Fig) Immunofluorescence staining for -catenin (C 6H) and its quantitative analysis (I) showed that fluoxetine reduced the intensity of -catenin significantly in the nucleus and marginally in the cell body at the medial and lateral compartments.', 'In addition, intraarticular administration of fluoxetine inhibited OA progression in the DMM-operated knee, but had no effect on integrity of the cartilage in the sham-operated knee ().', '(A, B) Three rats in each group had DMM and sham surgeries in the right and left knees, respectively, as described in .', 's006"">S6 FigModified Mankin score at the lateral compartment and larger areas of stained knee joint sections shown in .', 'The modified Mankin scores at the lateral compartments are lower than those at the medial compartments indicated in B.', '(B) Larger areas of stained sections of knee joints shown in A, 6C and 6F.']",10.1371/journal.pone.0184388.g006,yes
PMC9442524,Figure_5,oa_package/43/59/PMC9442524.tar.gz,['The average CT value for cancer and granuloma.'],"Figure 5 The average CT value for cancer and granuloma. CT, computed tomography; HU, Hounsfield units.",yes
PMC10659606,Figure_4,oa_package/31/6b/PMC10659606.tar.gz,['.'],Figure 4. Results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of differentially expressed genes (DEGs). (A) Results of GO enrichment analysis for ischemic stroke (IS). (B) Results of KEGG enrichment analysis for IS. (C) Results of GO enrichment analysis for myocardial infarction (MI). (D) Results of KEGG enrichment analysis for MI.,yes
PMC3812850,Figure_5,oa_package/1a/f9/PMC3812850.tar.gz,"['Normal ECUThe ECU has a flattened ovoid configuration in transverse section at the level of the ulnar groove and lies deep to the subsheath (figure 5A).', 'The extensor retinaculum is a thin structure around the periphery of ECU (figure 5B).', 'In supination the tendon demonstrates minor subluxation, lying towards the ulnar aspect of the groove (figure 5C).']","Figure5 Axial ultrasound images of the normal extensor carpi ulnaris (ECU) tendon. In wrist pronation (A) the tendon lies within the ulnar groove (white arrows). The subsheath (black arrows) is immediately superficial to the tendon and attaches to the ulna. Distal to the ulna (B) the tendon (curved white arrow) lies superficial to the meniscal homologue of the triangular fibrocartilage complex (asterix) and the triquetrum. The extensor retinaculum (broken black arrows) displays hyper-reflective and hyporeflective properties due to the effects of anisotropy. In wrist supination (C) the tendon (curved white arrow) moves to the ulnar aspect of the groove (white arrows), and a small area of echo bright fatty tissue lies in the radial aspect of the groove (black asterisk). The tendons of the fourth and fifth compartments (broken white arrows) now lie in closer relation to the ECU tendon.",yes
PMC10691688,Figure_6,oa_package/92/b7/PMC10691688.tar.gz,"['MMP9 deficiency increased the quantity of both V (A) and 3 (B) integrins in kidney cell lysates observed by western blotting, and of 3 integrin in collecting ducts cells observed by immunofluorescence (C).', 'The 3 integrin co-localized with periostin at the base of the cyst (D).', 'g006Effect of MMP9 on integrin expression.']",10.1371/journal.pone.0294922.g006,yes
PMC9206905,Figure_1,oa_package/bc/96/PMC9206905.tar.gz,"['9%) were found in both the supra-tentorial and infra-tentorial regions(), with 10 (13.', 'Coronal T2 (A) appears hypointense and post-contrast axial (B) and sagittal (C) images show multifocal ring enhancement in supratentorial and infratentorial conglomerate tuberculomas.', '']",Fig. 1 Coronal T2 (A) appears hypointense and post-contrast axial (B) and sagittal (C) images show multifocal ring enhancement in supratentorial and infratentorial conglomerate tuberculomas.,yes
PMC3033413,Figure_6,oa_package/9d/dd/PMC3033413.tar.gz,['g006Effect of IFN- deficiency on SEB-induced chemokine responses in vivo.'],10.1371/journal.pone.0016764.g006,yes
PMC9562795,Figure_1,oa_package/0d/a3/PMC9562795.tar.gz,['Evaluation of CBCT images from sagittal view.'],Figure 1 Evaluation of CBCT images from sagittal view. Maximum root length was measured from the apical point and the CEJ. Half of the measured length was determined as the midroot. Buccal bone thickness was measured at two points (crestaland midpoint).,yes
PMC8314799,Figure_1,oa_package/22/29/PMC8314799.tar.gz,[' A fat-attenuating mass at the gastric antrum protruding through the pylorus and into the first portion of the duodenum.'],Figure 1 A fat-attenuating mass at the gastric antrum protruding through the pylorus and into the first portion of the duodenum.,yes
PMC5662851,Figure_5,oa_package/4c/fa/PMC5662851.tar.gz,"['Another incidental finding we observed was myelomalacia in the cervicothoracic spine ().', 'A 46-year-old man presented with low back pain without any neurological symptoms.']",Fig. 5 A 46-year-old man presented with low back pain without any neurological symptoms. T2-weighted sagittal screening of the whole spine revealed degenerative disease at the C56 intervertebral disc that was causing significant spinal cord compression with myelomalacic changes at this level (arrow). The lumbar spine was unremarkable except for bilateral spondylolysis at LV5 with associated anterolisthesis of LV5 over SV1.,yes
PMC10533866,Figure_11,oa_package/76/8b/PMC10533866.tar.gz,[],"Fig 11 Anterior (A) and lateral (B) supine radiographs of a left knee following anterior cruciate ligament reconstruction using a bonepatellar tendonbone autograft with inside-out medial meniscal ramp repair and lateral meniscal double-tunnel transtibial root repair. The hardware is seen in good condition, including femoral 7 20mm and tibial 9 20mm cannulated titanium interference screws, and a cortical button (yellow circles).",yes
PMC8067891,Figure_3,oa_package/a5/9a/PMC8067891.tar.gz,"['MRCP demonstrates a T2 high signal intensity lesion in the left liver lobe accompanied with intrahepatic biliary duct ectasia and parenchymal shrinkage [].', 'T2-weighted abdominal magnetic resonance images in axial (a-d), coronal (e) and sagittal (f and g) planes demonstrate a T2 high signal intensity lesion in the left liver lobe accompanied with intrahepatic biliary duct ectasia and parenchymal shrinkage without signal change on fat saturation sequences (d-g) Magnetic resonance cholangiopancreatography image (h) shows similar findings as intrahepatic biliary duct ectasia of left liver lobe accompanied with an area of filling defect within dilated biliary ducts showing branching distributionCholangiocarcinoma versus focal cholangitis or hepatitis was in differential diagnosis based on MRCP.']","Figure 3 T2-weighted abdominal magnetic resonance images in axial (a-d), coronal (e) and sagittal (f and g) planes demonstrate a T2 high signal intensity lesion in the left liver lobe accompanied with intrahepatic biliary duct ectasia and parenchymal shrinkage without signal change on fat saturation sequences (d-g) Magnetic resonance cholangiopancreatography image (h) shows similar findings as intrahepatic biliary duct ectasia of left liver lobe accompanied with an area of filling defect within dilated biliary ducts showing branching distribution",yes
PMC10566271,Figure_1,oa_package/34/36/PMC10566271.tar.gz,"['The characteristics of DUDT wounds (A) differ from those of ischemic ulcers (pale, yellow, and cold with weak or absent pulse), neuropathic ulcers (preceded by callus formation), arterial ulcers (characterized by intense pain, punched-out appearance, shininess, decreased hair growth, pallor on leg elevation, weak or absent pulse, and delayed capillary refill), neuroischemic ulcers (with an abnormal ABI), and peripheral neuropathic ulcers.', '.', '1177_2632010X231205366-fig1"" position=""float""/>Ischemic UlcersIschemic ulcers result from inadequate blood flow, which causes local ischemia in the skin and the underlying tissues.', '\n9\n The patients exhibit typical clinical manifestations of ischemic ulcers, such as paleness, coldness, and the absence of a pulse\n5\n (B).', 'Depending on the severity of the PAD-related conditions, arterial ulcers can cause lesion expansion, extensive ischemia, infection, and, ultimately, amputation,\n24\n (C).', 'Neuropathic UlcersNeuropathic ulcers have a distinct appearance and are most frequently observed in the plantar region (D).', '9,15,27,28 The lateral malleolus, anterior tibia, toes, heels, and other bony prominences are the common sites for arterial ulcers\n21\n (E).', '9,31 Neuroischemic ulcers typically present with other signs of ischemia and changes in peripheral sensation\n18\n (F).', '\n33\n A carbuncle appears as a hard and painful red lump that rapidly grows to a diameter of 3 to 10 cm within a few days (G).', 'They commonly present with edema, venous dermatitis, varicosity, and lipodermatosclerosis (H).', '7% of patients with diabetes, the underlying cause of diabetic bullae or blister formation remains unidentified (I).', 'The symptoms of cellulitis include a warm, poorly defined area of redness beneath the skin, accompanied by edema and tenderness upon touch\n37\n (J).']","Figure 1. (A) DUDT, (B) ishemic ulcer, (C) arterial ulcer, (D) neuropathic ulcer, (E) mix ulcer, (F) neuro-ischemic, (G) furuncle/carbuncle, (H) venous ulcer, (I) diabetic bullae, and (J) cellulitis. Source: Data adapted from Suriadi, Cellulitis contributed by Suriadi.",yes
PMC10751940,Figure_2,oa_package/08/75/PMC10751940.tar.gz,"[', 2021) (; Table 1).']","FIGURE 2 Mechanism of Regulating Adult Hippocampal Neurogenesis by Active Ingredients of Traditional Chinese Medicine in Antidepressant. Active ingredients extracted from Traditional Chinese Medicine promote AHN by regulating various signaling pathways, such as BDNF signaling pathway, PI3K/Akt signaling pathway, and Wnt/-catenin signaling pathway, as well as by inhibiting neuroinflammation and modulating the HPA axis and microbiota-gut-brain axis. BDNF: Brain-derived neurotrophic factor. PI3K: Phosphatidylinositol 3-kinase. Akt: Protein kinase B. AHN: Adult hippocampal neurogenesis. NF-B: Transcription factor nuclear factor. NLRP3: Nod-like receptor thermal protein domain 3. CRH: Corticotropin releasing hormone. ACTH: Corticotropin. CORT: Cortisol. LPS: Lipopolysaccharide. RGL: Radial glial-like cells. HPA: hypothalamic-pituitary-adrenal.",yes
PMC3154159,Figure_8,oa_package/c9/60/PMC3154159.tar.gz,['Antioxidant enzyme synthesis in response to orange oil treatment.'],"Figure 8 . Immunoblot analysis demonstrating expression of HO-1, NQO1 and GCLm proteins at 6, 12 and 24 hrs following 15 min treatment of BEAS-2B cells with the oil preparation or time-matched soy oil control. Representative blots from one of three separate experiments are shown above. Densitometric evaluations of each target protein blot normalized to its corresponding GAPDH for all three experiments are provided below. Bars represent mean SEM.",yes
PMC4559407,Figure_4,oa_package/3a/90/PMC4559407.tar.gz,"['44 8Narrow symphysis that is indistinct from the bone surface, spongiosa ().', 'g004Right-sided metatarsal in dorsoplantar projection.', '5% of the bone elements have a symphysis with more distinction at the lateral and medial border but with a narrower epiphyseal-diaphyseal symphysis, showing animals that have reached AG 4 ().']",10.1371/journal.pone.0137109.g004,yes
PMC8580643,Figure_2,oa_package/47/0d/PMC8580643.tar.gz,['Laparoscopic view of resection.'],Figure 2 Laparoscopic view of resection.,yes
PMC11591815,Figure_11,oa_package/d5/39/PMC11591815.tar.gz,[],"Figure 11 MR Analysis of methylation sites of and risk of ischemic stroke. The scatter plot shows the size () of the SNP effect on the outcome ( -axis) and exposure ( -axis), 95% confidence interval. Each dot represents an SNP that is used as a genetic tool. The slope represents the estimate for each of the five different MR Tests. The SNP effect of each methylation site is expressed as an SD per 1 unit high, and for the outcome (ischemic stroke), they are expressed as a logarithmic probability per 1 unit high. ( ) Scatter plot of methylation site cg02631906 associated with ischemic stroke. ( ) Forest plot of methylation site cg02631906 associated with ischemic stroke. ( ) Leave-one-out analysis plot of methylation site cg02631906 associated with ischemic stroke. ( ) Funnel plot of the association of methylation site cg02631906 with ischemic stroke. ( ) Scatter plot of methylation site cg08483560 associated with ischemic stroke. ( ) Forest plot of methylation site cg08483560 associated with ischemic stroke. ( ) Leave-one-out analysis plot of methylation site cg08483560 associated with ischemic stroke. ( ) Funnel plot of the association of methylation site cg08483560 with ischemic stroke. IS: ischemic stroke; MR: Mendelian randomization; SNP: single nucleotide polymorphism; SD: standard deviation.",yes
PMC10693445,Figure_3,oa_package/dc/80/PMC10693445.tar.gz,"['Areas of T2-FLAIR hyperintensity posterior to C2 and C6 vertebral bodies (a, b)MRI T2-FLAIR thoracic spine sagittal view with and without IV contrast.']","Figure 3 MRI T2-FLAIR thoracic spine sagittal view with and without IV contrast. Multiple patchy areas of abnormal signal scattered in the thoracic spine, most prominent at T1-T4, T5-T6, and T9 (a, b)",yes
PMC4742117,Figure_5,oa_package/f2/f1/PMC4742117.tar.gz,"['Result of antigen stimulated Dendritic cell (DC) or Langerhans cell (LC) co-cultured with splenocytesWhen DCs and LCs were prepared from the mouse, the splenocytes were also harvested and frozen.']","Figure 5 Result of antigen stimulated Dendritic cell (DC) or Langerhans cell (LC) co-cultured with splenocytes When DCs and LCs were prepared from the mouse, the splenocytes were also harvested and frozen. The antigens were presented and allowed for uptake by the cells. Two days after the antigen stimulation to DCs and LCs, the splenocytes were thawed and placed in the incubator overnight. The cells were then co-cultured together on the third day, and cells were harvested next day and an antibody cocktail was used to stain the cells. In , the percentage of CD8a+ DCs and LCs when co-cultured with splenocytes at 12h and 24h. At 24 hours, there was significant percentage changes in the DCs-splenocytes co-cultured group between two peptides (mutant higher than wild-type and control, = 4, < 0.05) and there is no differences seen in LCs-splenocyte co-cultured group between two peptides at either time point. In , the percentage of peptide+ cells in CD8a+ cells was measured in the DCs and LCs co-cultured at 12h and 24h. Significances were found for both LCs and DCs cultures between the control, WT, and mutant peptides ( = 4, < 0.05), except for when comparing the control and WT groups in LCs ( > 0.05). In , the percentage of CD4+ T-cells were studied in the DCs and LCs co-cultures with splenocytes at 12h and 24h. No significances were found. In , the concentration of IFN-gamma was studied in the co-culture system at 12 hours and 24 hours. There is a significant difference in the DC population between the control and mutant antigen at the 24h time point ( = 4, < 0.05). There is also a significance between the mutant and control antigen in the LC population ( = 4, < 0.05).",yes
PMC10544197,Figure_4,oa_package/2a/89/PMC10544197.tar.gz,"['Gross distribution of Evans blue showed evidence of BBB breakdown in AslNeo/Neo mice (A).', 'Representative images were taken and used to measure Evans blue extravasation (B and Supplemental ).', 'Regions of interest (ROIs) were randomly selected around the proximal area of the blood vessels, and the fluorescence signal intensity was quantified by using the area average for the box plots (C) and the point-to-point function for line plots (, D and E).', 'Consistent with our MRI data, we found evidence of BBB breakdown in AslNeo/Neo mice as shown by a significant increase of Evans blue dye extravasation (D).', 'BBB breakdown in AslNeo/Neo mice as shown by Evans blue fluorescent tracer extravasation.']","Figure 4 BBB breakdown in mice as shown by Evans blue fluorescent tracer extravasation. ( ) Representative picture of gross distribution of Evans blueconjugated albumin after I.V. injection in WT, mutant ( ), and sodium nitritetreated mutant mice ( + NO). ( ) Two-photon images of cerebral vasculature shown as (i) maximal intensity plots and (ii) fluorescence intensity maps. Box (50 50 m) plots and line (70 m) profile of fluorescence intensity were measured at the designated locations. The box color corresponds to the genotype legend in , , and . ( ) Box plot ROI intensity (WT: 42.3 1.9 A.U./m . : 125.4 3.4 A.U./m . + NO: 104.1 1.9 A.U./m .) WT: = 4 mice, 36 images per mouse, 68 ROIs per image. Total 123 ROI determinations. : = 4 mice, 35 images per mouse, 68 ROIs per image. Total 116 ROI determinations. + NO: = 5 mice, 35 images per mouse, 68 ROIs per image. Total 134 ROI determinations. Mean SE. ( ) Line profile of fluorescence intensity. Extravascular fluorescence levels were estimated by the area under the curves of the pixel intensity by subtracting that of blood vessel. ( ) The extravascular AUC. (WT: 36,179 3,836 A.U. : 86,453 10,419 A.U. + NO: 55,036 3,377 A.U.) Line profiles were presented as the pixel intensity. WT: = 4 mice, 4-line profiles per image, 16 image determinations. : = 4 mice, 4-line profiles per image, 21 image determinations. + NO: = 5 mice, 4-line profiles per image, 14 image determinations. Bar graphs represent mean values while error bars represent the SE. * < 0.05, *** < 0.001, and **** < 0.0001. One-way ANOVA followed by Newman-Keuls test. I.V., intravenous.",yes
PMC6111199,Figure_5,oa_package/45/49/PMC6111199.tar.gz,"['Histopathological examination of the heart tissues of rats stained with Hematoxylin and Eosin showed that hypoxia induced a marked degeneration of myocardial cells and widening of the endomysium due to vascular congestion and cellular infiltration, while treatment with Melat, Querc, and their combination markedly improved the myocardial cells and markedly decreased of the cellular infiltration, especially in rats treated with a combination of Melat and Querc ().', 'Light photomicrographs of heart from rat stained with Hematoxylin and Eosin (Scale bar: 100 m), in which (A) represent normal myocardial cells (arrowhead) and endomysium (arrow).']","Fig. 5 Light photomicrographs of heart from rat stained with Hematoxylin and Eosin (Scale bar: 100m), in which (A) represent normal myocardial cells (arrowhead) and endomysium (arrow). (B) Section of heart from rat exposed to hypoxia showing marked degeneration of myocardial cells (arrowhead) and widening of the endomysium due to vascular congestion and cellular infiltration (arrow). (C, D and E) are sections of heart from rat exposed to hypoxia and received Melat, Querc and combination of both of them respectively, showing marked improvement of the myocardial cells and marked decrease of the cellular infiltration specially in rats received combination of Melat and Querc (E).",yes
PMC10807876,Figure_2,oa_package/10/04/PMC10807876.tar.gz,"['00178-g001"" position=""float""/>Labeled cross-sectional US image of the right groin demonstrating a large, hypoechoic mass causing mass effect on the common femoral vein near its communication with the greater saphenous vein.']","Figure 2 Labeled cross-sectional US image of the right groin demonstrating a large, hypoechoic mass causing mass effect on the common femoral vein near its communication with the greater saphenous vein.",yes
PMC8808045,Figure_8,oa_package/1f/7b/PMC8808045.tar.gz,"['C) was a 67-year-old woman presenting a larger (~5 cm) flat lesion on her left breast ().', 'Case 2.']",Figure 8 Case 2. ( and ) Macro-photo and dermoscope picture of large seborrheic keratosis on left breast before HIFU. ( and ) Macro-photo and dermoscope picture of treated area at control visit 4 weeks after HIFU treatment.,yes
PMC10486883,Figure_4,oa_package/cc/a2/PMC10486883.tar.gz,"['0 mm was introduced in the anterior recess of the superior joint space, and it was used for instrument passage, drainage, and LBs evacuation ().', 'Triangulation technique and LBs washout from the second cannula, which can be seen lying on the patient s face.']","Figure 4 Triangulation technique and LBs washout from the second cannula, which can be seen lying on the patients face.",yes
PMC3701899,Figure_2,oa_package/24/4b/PMC3701899.tar.gz,"['Manumycin A (20 M) showed high luciferase activity in the presence of the expanded CUG repeat (a and ', 'Results from RT-PCR showed that the addition of manumycin A effectively corrects aberrant splicing of Clcn1 in the presence of the expanded CUG repeat (b,c).', 'Percentages of Clcn1 exon 7A inclusion showed that manumycin A treatment rescued abnormal exon 7A inclusion levels caused by expression of the expanded CUG repeat to levels similar to those for the normal CUG repeat (c).', 'S3) compared to the control (c).', 'Importantly, manumycin A treatment showed reduced total amount of Clcn1 mRNAs (with and without exon 7A) (b and ', 'org/1999/xlink"" xlink:href=""srep02142-f1""/>Identification of small-molecule compounds that correct aberrant splicing of Clcn1.']","Figure 2 Identification of small-molecule compounds that correct aberrant splicing of . (a) A luciferase reporter assay showed that manumycin A corrected aberrant splicing in the presence of the expanded CUG repeat (mean + SEM, n = 3). (b) Cellular splicing analysis showed that manumycin A corrects aberrant splicing of . (c) Quantification of the results shown in (b) (mean + SEM, n = 3). (d) Structure of manumycin A. The gel image was cropped around the region of interest and the samples (n = 3) were resolved in the same gel. Statistical significance was determined using -tests (* < 0.05, ** < 0.01).",yes
PMC7640573,Figure_4,oa_package/20/db/PMC7640573.tar.gz,"['Following the discovery of the ASD, we realized transesophageal echocardiography (TOE) which demonstrated PFO with an enclosed floating thrombus ().', 'TOE revealing a mobile thrombus inserted in the PFO.']","Fig. 4 TOE revealing a mobile thrombus inserted in the PFO. LA = left atrium, RA =right atrium.",yes
PMC3188523,Figure_4,oa_package/72/4c/PMC3188523.tar.gz,"['Both displayed similar basal levels of cytokine mRNA, however, there were significantly higher levels of IL-17 and IFN- in stimulated Why1 T cells than in BL/6 T cells (\nA,B\n), while levels of IL-4 were higher in BL/6 T cells than in Why1 T cells (\n', 'g004IRAK-2 is necessary for CD4 T cell-specific IL-17 production.', 'We observed a significant reduction in IL-17 mRNA induced by IRAK-2-specific (but not by control shGFP) hairpin treatment in Why1 T cells (\nD\n).', 'Knockdown of IRAK-2 also suppressed IL-17 by BL/6 T cells (\nD\n), indicating that its effect is not specific to MOLF mice.', 'Interestingly, there was no significant effect on IFN- (\nE\n) or IL-4 (\n', 'Knockdown of IRAK-2 in BL/6 and Why1 T cells was confirmed by mRNA analysis due to the lack of a suitable antibody against IRAK-2 (\nG\n).', 'Why1 T cells stimulated with anti-CD3/CD28 produced significantly more IL-17 than their BL/6 counterparts (\nH\n).', 'Furthermore, knockdown of IRAK-2 significantly decreased IL-17 production in both Why1 and BL/6 T cells at 48 and 96 hours following stimulation, indicating that this effect was stable over time (\nI, J\n).']",10.1371/journal.ppat.1002272.g004,yes
PMC7385069,Figure_4,oa_package/67/56/PMC7385069.tar.gz,"['The overall block diagram of the proposed model.', '\nThe overall block diagram of the proposed model is given in ']",Fig. 3 An original and augmented sample from the training set.,yes
PMC6853273,Figure_9,oa_package/d0/cd/PMC6853273.tar.gz,[],Figure 9 Postoperative sagittal MRI demonstrating resection of the intraorbital and intramuscular tumor component * = Areas of prior tumor invasion of the lateral orbit and temporalis muscle MRI: magnetic resonance imaging,yes
PMC11314049,Figure_9,oa_package/2e/26/PMC11314049.tar.gz,"['Considering the critical factors affecting the transdermal capabilities of PtNPs, dermatologists often supplement natural penetration abilities with additional delivery mechanisms ().', 'For example, by utilizing microneedling (a) or radiofrequency techniques, the barrier efficacy of the stratum corneum can be bypassed through the creation of microchannels in the skin, thereby enhancing nanoparticle delivery.', ', electrophoresis, iontophoresis, and electroporation [168,169]) (b), and diffuse mechanical stresses, such as ultrasound [170,171] (c), can also help to increase skin permeability.', 'Moreover, chemical enhancers that alter the stratum corneum lipid structure can further facilitate skin penetration (d) [172].', '(a) Methods of drug delivery to the skin using microneedles (MNs).']","Figure 9 ( ) Methods of drug delivery to the skin using microneedles (MNs). Microneedles are first applied to the skin ( ) and then used for drug delivery ( ). Solid microneedles are used as a pretreatment, after which the drug can diffuse through residual holes in skin from a topical formulation (solid MNs). After insertion of drug-coated microneedles into the skin, the drug coating dissolves off the microneedles in the aqueous environment of the skin (coated MNs). Drug-loaded microneedles are made of water-soluble or biodegradable materials encapsulating the drug that is released in the skin upon microneedle dissolution (dissolving MN). Hollow microneedles are used to inject liquid formulations into the skin (hollow MNs). Reproduced with permission [ ]. Copyright 2012, Elsevier. ( ) A schematic showing the pathways of topical and transdermal delivery, including electrically assisted delivery by iontophoresis, electroporation, or electroincorporation. Reproduced with permission [ ]. Copyright 1999, Elsevier. ( ) Schematic of various mechanisms of ultrasound-triggered release of drug agents from nanoparticles as well as drug transport. Reproduced with permission [ ]. Copyright 2023, Elsevier. ( ) Schematic of diffusionpartitionsolubility actions of skin penetration enhancers. Reproduced with permission [ ]. Copyright 2013, Elsevier.",yes
PMC10943328,Figure_2,oa_package/80/76/PMC10943328.tar.gz,"['51\n depicts a case of oral candidiasis while using anti-IL17.', 'Oral candidiasis with the use of anti-IL17.', 'The risk of systemic fungal infections with anti-IL23 treatment in patients with psoriasis was investigated in 16 randomized controlled trials.']",Figure 2 Oral candidiasis with the use of anti-IL17.,yes
PMC3419240,Figure_7,oa_package/0f/9c/PMC3419240.tar.gz,"['05; A).', 'No changes in interneuron markers were observed in presymptomatic R6/2 mice (B).', '05; C).', '0004) was significantly decreased compared WT mice at this age (D).', 'g007Transcriptional profile of interneuron markers.']",10.1371/journal.pone.0042878.g007,yes
PMC9838765,Figure_2,oa_package/83/df/PMC9838765.tar.gz,[],"FIGURE 2 Clinical, dermoscopic, reflectance confocal microscopy (RCM), and histologic characteristics of a 2mm pigmented basal cell carcinoma. Panel A shows the location on the left upper forehead (black arrow). Panel B indicates dermoscopic features of the same lesion, with the presence of spoke wheel structures and bluegrey dots. Panel C demonstrates a tumor island (red star) with palisading nuclei (yellow arrow) and clefting (red arrow), visualized with RCM. Panel D is the lesion's dermatopathology slide (8x), containing basaloid nests of hyperchromatic nuclei (red star) with peripheral palisading nuclei (yellow arrow) and visible clefting",yes
PMC3516567,Figure_1,oa_package/b5/52/PMC3516567.tar.gz,"['At all concentrations and time points tested, TcdB failed to activate caspase-3 and -7, central regulators in apoptotic cell death (A).', 'g001TcdB induces necrosis in epithelial cells.', 'g001""/>Despite the lack of caspase-3/7 activation, the TcdB treatments had a significant impact on cellular ATP levels (B).', '5 hours had completely lost their membrane integrity (C).', '5 hours after intoxication and at an increased level after 8 hours (D).', '1 nM, consistent with the cell death data obtained with an ATP indicator (B).', 'We found that at 10 nM TcdB, HMGB1 was released into the cytoplasm after 1 hour (E).', '1003072-QaDan1"" ref-type=""bibr"">[18], TcdB did not trigger the induction of apoptosis in cultured epithelial cells as measured by caspase-3/7 activation (A, S2B), and HMGB1 release (E) support this conclusion.']",10.1371/journal.ppat.1003072.g001,yes
PMC7515668,Figure_2,oa_package/a3/35/PMC7515668.tar.gz,"['8%) were detected and removed by the surgeons ().', '.']","Fig. 2. A lymph node tattooed with charcoal suspension. A. In the operative field, a lymph node tattooed with charcoal suspension can easily be identified as a dark nodular lesion. B. A resected specimen shows multiple charcoal-tattooed lymph nodes. C. A low-power-field (H&E staining, 100) microscopic view shows tattoo pigment (arrows) along the periphery of the metastatic lesion.",yes
PMC8422266,Figure_1,oa_package/c9/0e/PMC8422266.tar.gz,"['[20,21] These procedures are based on short series of cases but are rapidly extending in light of the promising results ().', ':Tips and Tricks for Valve-in-valve Procedures: Valve Fracturing and BASILICA\nA: Micro-CT analysis of ACURATE neo device (Boston Scientific) used for the valve-in-valve procedure showing difference of expansion before (13.']",Figure 1: Tips and Tricks for Valve-in-valve Procedures: Valve Fracturing and BASILICA,yes
PMC3779769,Figure_3,oa_package/5e/01/PMC3779769.tar.gz,"['After reviewing theses areas on T1-weighted images of the routine study, theses were confirmed to be hemangiomas ().', '(A) Sagittal T1 image showing abnormal hyperintense signal at L3 vertebral body.']",Fig. 3 Sagittal T1 image showing abnormal hyperintense signal at L3 vertebral body. T2-weighted image again redemonstrates hyperintense signal at L3. Fat sat images showing signals drop suggestive of focal fatty deposit. Differentiation was not possible only on screening protocol. HL means Head first with left side image.,yes
PMC7423909,Figure_1,oa_package/c5/b1/PMC7423909.tar.gz,['.'],Figure 1. Liver biopsy showing bridging necrosis with extensive hepatocyte dropout and small aggregates of plasma cells (arrows).,yes
PMC2064547,Figure_9,oa_package/11/40/PMC2064547.tar.gz,"['Compared with wild-type mice, APP23 mice show a 30% reduction in NeuN-positive cells in the entorhinal cortex, whereas APP23/TNFR1\n / mice show no significant reduction at 24 mo of age (, A and C).', 'Results were similar in the hippocampus, where APP23 mice had 15% fewer NeuN-positive cells in the CA1 area of the hippocampus ( B, D; Calhoun et al.', ', 1998), whereas little neuronal loss was seen in APP23/TNFR1\n / mice at 24 mo of age (, B and D).', '.']","Figure 9. (A) Fewer NeuN-positive neurons were present in the entorhinal cortex of APP23 mice at the age of 24 mo. (B) NeuN immunostaining demonstrated little neuronal loss in the CA1 field in APP23/ mice. (C) Statistical analyses show that APP23 mice had significantly fewer neurons in the entorhinal cortex at 24 mo of age (*, P < 0.01). (D) Statistical analyses show APP23 mice had significantly fewer neurons in the CA1 of the hippocampus at 24 mo of age (*, P < 0.01). Error bars represent SD. Bars, 10 m.",yes
PMC4112974,Figure_4,oa_package/5a/06/PMC4112974.tar.gz,['Effect of overexpression or knockdown of PAL31 in C6 on H2O2-induced toxicity.'],"Figure 4 Representative micrographs showing C6 overexpressing GFP or GFP-tagged PAL31 after being treated with H O (0~1mM) for 4hours. MTT assay in GFP- or GFP-PAL31-overexpressed C6 after H O treatment showing significant difference (cytoprotective effect of PAL31) at 0.5mM and 1mM H O treatment between C6/GFP and C6/PAL31 groups. The data in each dosage were analyzed by two-way ANOVA and Bonferroni post hoc test. *P<0.05, GFP (+H O ) compared to PAL31 (+H O ), n=4, at 0.5mM and 1mM. Western blot analysis of pal31 siRNA-treated C6 showing knockdown of PAL31 expression by PAL31 silencer using 41.5 (lane1), 83 (lane2), and 166 (lane 3) picomole of pal31 siRNA or 332 picomole negative control. Actin works as a loading control. MTT assay in Negative- or PAL31 silencer transfected C6 after H O treatment showing significant difference at 1mM H O treatment between C6/Negative and C6/PAL31 siRNA groups. *P<0.05, Negative (+H O ) compared to PAL31 siRNA (+H O ), n=4. Magnification 100X .",yes
PMC8071095,Figure_5,oa_package/77/42/PMC8071095.tar.gz,['Endoscopic retrograde cholangiopancreatography cholangiogram shows complete clearance of common bile duct.'],Figure 5 Endoscopic retrograde cholangiopancreatography cholangiogram shows complete clearance of common bile duct.,yes
PMC8258789,Figure_4,oa_package/51/8c/PMC8258789.tar.gz,['The patient was then addressed to the Endocrine Unit for biochemical evaluation of the mass.'],"Fig. 4 (A-E). Magnetic resonance imaging (MRI) confirmed the anatomic relationships of the mass as seen on CT. On T1-weighted images the lesion was isointense (4A) without drop of signal in T1 weighted opposition phase sequence (4B). In fat-suppressed fast spin-echo T2-weighted sequence (4C) and in FIESTA sequence (4D) the lesion appeared mildly and heterogeneous hyperintense. After contrast medium injection it is characterized by a progressive, heterogeneous contrast enhancement ( E).",yes
PMC3862626,Figure_2,oa_package/2a/b1/PMC3862626.tar.gz,"['Poor Eyes test performance showed association with thinning of 5 cortical areas (\n\n\n, \n\nTable 3\n\n).', 'g002Cortical areas of which thickness correlated with Eyes test performance in patients with multiple sclerosis.']",10.1371/journal.pone.0082422.g002,yes
PMC6749640,Figure_2,oa_package/9f/2f/PMC6749640.tar.gz,"[' 2), High definition SD-OCT (Optovue RTVue XR Avanti, Optovue Inc.', 'eye with solar maculopathy shows a hyporeflective spot in the center of the macula\nAn early picture of solar maculopathy (2 weeks after sun exposure), a in the right eye and b in the left eye\n']",Fig. 2 A fundus auto fluorescence image of Rt.eye with solar maculopathy shows a hyporeflective spot in the center of the macula,yes
PMC3680301,Figure_1,oa_package/36/b6/PMC3680301.tar.gz,['Cardiac magnetic resonance imaging confirming the diagnosis of tetralogy of Fallot.'],Figure 1 Ventricular septal defect and overriding aorta over the septum. Right ventricular hypertrophy and subpulmonary stenosis.,yes
PMC4255369,Figure_5,oa_package/67/0f/PMC4255369.tar.gz,['Mapping of domain on tau protein recognised by DC8E8.'],"Figure 5 For the epitope mapping of DC8E8 monoclonal antibody, we used full-length three- and four-repeat tau isoforms with two N-terminal inserts ( and ), tau deletion mutants ( ) and tau-derived synthetic peptides ( ). All deletion mutants that contained the microtubule-binding repeat (MTBR) region ( ) were recognized by DC8E8; tau deletion mutants lacking the MTBR region were not recognized ( and ). Importantly, DC8E8 recognized each of synthetic peptides derived from individual MTBRs ( ). The result is that DC8E8 recognised four binding sites (epitopes) located in the MTBR region of the tau protein, and each epitope is separately located within one MTBR. To narrow down the DC8E8 minimal epitope, homologous peptides derived from the tau protein repeat region (MTBR14) were analysed in competitive enzyme-linked immunosorbent assays. Tau peptides containing at least six amino acids of the DC8E8 recognition sequence HXPGGG were capable of competing with mis-disordered tau (amino acids 151391) for binding to antibody DC8E8. Tau peptides containing five amino acids of the DC8E8 recognition sequence did not compete with mis-disordered tau for binding to antibody DC8E8. Schema of epitopes on tau protein molecule recognised by DC8E8. The DC8E8 monoclonal antibody is capable of binding four separate binding regions, with each region forming one individual epitope. These four epitopes are separately located within the first, second, third and fourth MTBR of protein tau. : All listed molecules are numbered in reference to the longest human tau isoform (2N4R) sequence.",yes
PMC11476495,Figure_3,oa_package/85/e1/PMC11476495.tar.gz,"[' 3).', ' 3).', 'After one dose of steroids with cytoplasmic vacuolations (arrows) suggestive of drug-induced myocardial injury\nDiscussionThis is the first reported case of suspected biopsy-proven Tbt-induced myocardial injury with prior anthracycline exposure demonstrating severe multi-organ failure including rhabdomyolysis, renal, and liver failure.']",Fig. 3 H&E stain of Endomyocardial biopsy (200x). After one dose of steroids with cytoplasmic vacuolations (arrows) suggestive of drug-induced myocardial injury,yes
PMC11752094,Figure_1,oa_package/5a/49/PMC11752094.tar.gz,['Clinical stroke and lacune pathology are associated with fewer tau pathologies.'],"Figure 1 Clinical stroke and lacune pathology are associated with fewer tau pathologies. (A) Effects of remote stroke history on Braak NFT stage in whole neuropathologicallyexamined subjects ( =4512), adjusted for age at death, sex, race, APOE4, and cognitive/dementia status. Effects of vascular pathologies, infarcts and lacunes (B) or One or more lacunes (C), on Braak NFT stage in subjects with ADtype dementia ( =2630), adjusted for age at death, sex, race, and APOE4. Data are presented as the adjusted meanstandard error of the mean. <0.01, and <0.001; by Student's test.",yes
PMC11328004,Figure_374,oa_package/e2/f1/PMC11328004.tar.gz,[],"Figure 68 (a) PLIM images of HeLa cells stimulated with cisplatinat differentconcentrations (1, 5, 10, 20, and 40 g mL ) for 4 h in two different sensing approaches. In the LbR approach,the cells were incubated with Az-DEVDGK-Nor (5 M, 30 min) andthen simultaneously treated with complex (5 M,30 min) and RhTz (5 M, 30 min) before stimulation withcisplatin. In the LaR approach, the cells were incubated with Az-DEVDGK-Nor(5 M, 30 min) followed by stimulation with cisplatin. Complex (5 M) and RhTz (5 M) were addedto the culture medium after 3 h and incubated for another 1 h. Scalebar = 20 m. (b) Relative occurrence of long-lived and short-livedsignals during PLIM. (c) Averaged lifetime values of the PLIM imagesin (a). Error bars represent the standard deviations of three independentmeasurements. (d) Enlarged views of the selected area in (a) and photoluminescencedecay curves of the circled area. Adapted with permission from Ref ( ). Copyright 2020 AmericanChemical Society.",yes
PMC11473614,Figure_1,oa_package/ab/39/PMC11473614.tar.gz,"['At week 12, no significant differences were observed in serological markers or body weight between the DFS group and the control group (A-1C) (p 0.', '01) (D).', '01) (E).', '--PMC9771309-->36550793\nEffects of high-temperature processed feed on rat body weight and liver index.']","Fig. 1 Effects of high-temperature processed feed on rat body weight and liver index. ( ) Serum lipid levels [* < 0.05, TC: comparison between HFD group and the other two groups; # < 0.05, TG: comparison between HFD group and the other two groups]; ( ) Serum enzyme levels [* < 0.05, ALT: HFD group compared to the other two groups; # < 0.05, AST: HFD group compared to the other two groups]; ( ) Body weight [** < 0.01, ## < 0.01, HFD group compared to the other two groups]; ( ) Liver index [** < 0.01, ## < 0.01, HFD group compared to the other two groups; & < 0.05, control group compared to DFS group]; ( ) Liver morphology across the three groups, with all experiments replicated six times.",yes
PMC2820417,Figure_3,oa_package/4c/27/PMC2820417.tar.gz,"['FISH with a double-labeled probe showed that expression of mutant APP induced chromosome mis-segregation and the development of aneuploidy for both chromosome 21 and 12 (, A C), but did not induce an increase in tetraploid cells (D).', '.', 'Comparing these results with the data of indicates that A peptides exert a general disruptive effect on chromosome segregation during mitosis that includes but is not restricted to chromosome 21.']","Figure 3. Chromosome aneuploidy induced in cells expressing either NL-APP K595N/M596L and V642I or V717F mutant . hTERT cells were transfected with expression vectors for the mutant human APP genes with the empty vectors pcDNA3 or pAG3 serving as control. The transfected cells were analyzed 48 h later. Expression of caused many cells to become aneuploid, as indicated by trisomy 21 and trisomy 12 (AC). expression failed to induce a significant increase in tetrasomy for chromosome 16 (D).",yes
PMC10026180,Figure_1,oa_package/9d/3a/PMC10026180.tar.gz,"['Multiple well demarcated, punched-out, ulcers with erythematous indurated borders, with fibrin on their base and an overlying hemorrhagic crust, on the lower leg of a 13.']","Figure 1 Multiple well demarcated, punched-out, ulcers with erythematous indurated borders, with fibrin on their base and an overlying hemorrhagic crust, on the lower leg of a 13.5-year-old boy with a combined immunodeficiency syndrome, consistent with varicella-zoster virus infection overlying chronic ulcerative granulomas.",yes
PMC9102204,Figure_1,oa_package/be/59/PMC9102204.tar.gz,"['Early recurrence of the keloid is summarized in Table 2, and the effectiveness and cosmetic results are visually demonstrated in s 1 3.', ' 1.', '1177_20595131221098531-fig1"" position=""float""/> 2.']",Figure1. The keloid on the ear treated with surgical shave excision followed by one set of triamcinolone acetonide and onabotulinumtoxinA injections. The length of follow-up time for resolved keloid was 49 days.,yes
PMC7195800,Figure_3,oa_package/51/0d/PMC7195800.tar.gz,"[' 3).', 'A convolutional neural network (VNet architecture) was trained on arterial phase images of a dynamic contrast enhanced MRI dataset to automatically segment enhancing breast lesions.', '130 patients in total were recruited for the training of the modelA more recent approach is the delineation of the different physiologically distinct regions (e.']",Fig. 3 A convolutional neural network (VNet architecture) was trained on arterial phase images of a dynamic contrast enhanced MRI dataset to automatically segment enhancing breast lesions. Two examples are shown (the worst and the best) with an average DICE coefficient of 0.820.15. The pink color denotes the pixels that where considered from the network as a lesion while the white pixels where corresponding to the radiologists segmentation used as the ground truth. The DICE coefficient is defined as 2 * the Area of Overlap between the pink and white areas divided by the total number of pixels in the segmentation mask. 130 patients in total were recruited for the training of the model,yes
PMC8409213,Figure_1,oa_package/26/08/PMC8409213.tar.gz,['Morphology of cervical lesions.'],Figure 1 A: Cervical lesions under colposcopy. White arrow indicates cervical extramedullary plasmacytoma lesions; B: Results of visual inspection with acetic acid. White arrow indicates positive visual inspection with acetic acid for extramedullary plasmacytoma; C: Cervical iodine test results. White arrow indicates negative staining.,yes
PMC10441467,Figure_1,oa_package/9a/87/PMC10441467.tar.gz,"['The volumetric measurements of BASC-identified clusters were then extracted and used as input biomarkers to the SuStaIn model (a, Additional file 1: Table S1).', 'Subtype and Stage Inference modellingWe utilized the w-scored volumetric measurements of 13 BASC-identified clusters (a, Additional file 1: Table S1) as input biomarkers for training the SuStaIn model (A metal stent insertion via trans-colonic access in a 69-year-old male.']",Fig. 1 A metal stent insertion via trans-colonic access in a 69-year-old male. The portal phase of the initial computed tomography (CT) demonstrates a dilated pancreatic duct (arrow). CT obtained during the procedure demonstrated the Chiba needle (Cook; arrow) in the trans-colonic access route. The dilated pancreatic duct (arrowhead) is opacified with contrast media injected via the Chiba needle. A ductogram revealed an abrupt cutoff (arrow) of the dilated pancreatic duct. Through-and-through access (arrow) is established using a percutaneous transhepatic biliary drainage tract. A bare metal stent (arrows) is placed at the pancreaticojejunostomy. Final ductogram displaying the flow of contrast media into the jejunum (arrow). The portal phase of the follow-up CT demonstrates a metal stent in the pancreatic duct (arrowhead) with an improved state of dilated pancreatic duct (arrow).,yes
PMC10570539,Figure_3,oa_package/e1/44/PMC10570539.tar.gz,"['DiscussionECD is a rare, multisystem disorder with approximately 1500 cases reported in the literature.']",Fig. 3 (G-H) Bone biopsy showing infiltration of foamy histiocytes in a mildly fibrotic background (Hematoxylin and Eosin stain; original magnification: 100 and 200).,yes
PMC5606113,Figure_7,oa_package/72/73/PMC5606113.tar.gz,"['7a).', '7b, these two methods produced the same result, which agrees well with the ERG data.', '7b).', 'Morphometric analysis of the effect of S1R knockout on PR survival in rd10 mice.', '01\nIn view of the profound S1R influence on rod survival and function, a follow-up question is whether S1R actually expresses in rods.']","Fig. 7 Morphometric analysis of the effect of S1R knockout on PR survival in rd10 mice. . Hoechst staining of nuclei on retinal sections collected at indicated time points. ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer. Scale bar 20m. . Quantification of ONL thickness and nuclei numbers: MeanSE, =34 mice; * <0.05, ** <0.01",yes
PMC3207855,Figure_7,oa_package/b4/c7/PMC3207855.tar.gz,"['Saline treated NPC2 / mice had intra-alveolar and interstitial accumulation of foamy macrophages and intra-alveolar accumulation of Periodic Acid-Schiff (PAS) positive material (), consistent with alveolar lipoproteinosis.', 'g007Histochemical and immunohistochemical analysis of NPC2 replacement therapy in murine lung sections.']",10.1371/journal.pone.0027287.g007,yes
PMC3677647,Figure_3,oa_package/38/df/PMC3677647.tar.gz,"['To confirm the diagnosis of mixed type I and II choledochal cyst, we reviewed pictures from the previous laparoscopic cholecystectomy to identify the cystic duct ().', '002""/>Laparoscopic cholecystectomy picture (a).']",Figure 3 Laparoscopic cholecystectomy picture (a). The black arrow shows a narrowed cystic duct that opens directly into the diverticulum (D) before cholecystectomy. Cholangiogram (b): red arrow shows a diverticulum with end of cystic duct after cholecystectomy; white arrow shows CBD which is not filled yet with contrast. (c) Intraoperative finding.,yes
PMC7102087,Figure_11,oa_package/b7/a2/PMC7102087.tar.gz,[],"Fig.11. Recurrent desmoid tumors in a patient affected by Gardner syndrome who underwent multivisceral transplantation. Some solid, round, or ovoid masses ( ) are visible in the ( ) upper and ( ) lower abdomen. Masses display clear edges and strong contrast enhancement. The pelvic tumor shows nonhomogeneous enhancement ( ); because it was not radically resectable, it was controlled with radiofrequency ablation and partial necrosis of tumor tissue.",yes
PMC4564926,Figure_1,oa_package/54/af/PMC4564926.tar.gz,"[""8 cm enhancing left upper pole renal mass that extended to the splenic renal hilum, pushed the pancreatic tail medially, and was inseparable from Gerota's fascia representing primary renal malignancy []."", 'Computerized tomography (CT) axial postcontrast (a) and CT coronal postcontrast (b) shows a large enhancing left upper pole renal mass (arrows)Axial contrast-enhanced (a) and coronal contrast-enhanced (b) computerized tomography scan shows an aortocaval lymph node enlargement (arrows)Fused axial single-photon emission computerized tomography (SPECT)- computerized tomography (CT) (a) and Fused coronal SPECT-CT (b) images 4 days after injection of In-111 ProstaScint shows abnormal uptake in the exophytic mass arising from superior pole of left kidney (arrows)Axial single-photon emission computerized tomography (SPECT), (a) axial computerized tomography (CT) (b) and fused axial SPECT-CT (c) images 4 days after injection of In-111 ProstaScint show increased radiotracer uptake in aortocaval lymph node (cross hairs)DiscussionProstate-specific membrane antigen is a type II membrane glycoprotein that was initially characterized by the murine monoclonal antibody (mAb) 7E11.']",Figure 1 Computerized tomography (CT) axial postcontrast (a) and CT coronal postcontrast (b) shows a large enhancing left upper pole renal mass (arrows),yes
PMC3196813,Figure_4,oa_package/8d/0a/PMC3196813.tar.gz,"['Noninfected Rag1 / mice displayed little or no evidence of intestinal lesions (A and B).', 'However, transfer of IEL in combination with LP triggered ileal lesions in Rag1 / recipients (A and B), and pathology closely resembled that seen in infected WT mice (e.', 'We found no evidence of bacterial translocation after transfer of IEL or LP into Rag1 / recipients (C).', 'In marked contrast, co-transfer of IEL and LP cells resulted in increased bacterial translocation into lamina propria regions of the Rag1 / small intestine (C).', 'S2E and A).', 'Pathology scores in the latter group were similar to scores obtained from transfers that were performed when both LP and IEL compartments were obtained from infected mice (B).', 'IEL and LP from infected mice synergize to induce intestinal lesions and bacterial translocation after transfer into uninfected Rag1 / recipients.']","Figure 4 IEL and LP from infected mice synergize to induce intestinal lesions and bacterial translocation after transfer into uninfected recipients. IEL and LP were isolated from day 4 infected WT mice and transferred alone or in combination into mice. Intestines were harvested 5 days after transfer, fixed, stained and examined for inflammatory changes. Groups of noninfected mice received no WT cells, LP alone, IEL alone or equal numbers of the two cell types (Panel A). Scale bar denotes 50 m. Pathology scores from individual mice in each group are shown in B (*, p<0.05). The experiment was repeated twice with the same result. Noninfected animals received IEL, LP or IEL + LP from Day 4-infected WT mice. Small intestines were collected 5 days after transfer and tissue sections were subjected to fluorescence in situ hybridization (FISH) analysis employing a Cy3-labelled 16S ribosomal RNA pan-bacterial probe (red) to detect the presence and localization of intestinal bacteria (Panel C). The staining also includes a 6FAM-labeled nonsense probe so that non-specific binding is visible as yellow fluorescence. Cell nuclei were counterstained with DAPI (blue). The scale bar in represents 50 m. This experiment was performed three times with the same result.",yes
PMC5640623,Figure_1,oa_package/af/bb/PMC5640623.tar.gz,"[' 1A).', ' 1B).', ' 1C).', 'Liver morphology in control (CNT), E2 and Testos groups.', '\nMetabolomic ChangesThe first step in metabolome analyses involved global analysis of all the 186 metabolites in the three groups.']","Figure 1 Liver morphology in control (CNT), E2 and Testos groups. Data are shown as meanSEM. ( ) Photomicrographs of liver at 400x magnification (H.E. staining). Black arrows point lipid droplets and black dashed circles indicate binucleated cells. ( ) Lipid droplets score in hepatocytes. ( ) Counting of binucleated cells per mm . Asterisks denote significant differences from control.",yes
PMC5794919,Figure_5,oa_package/a6/e7/PMC5794919.tar.gz,"['Comparing Simulated and Acquired Data 5 compares simulated data with actually acquired undersampled data of three volunteers (15.', 'Comparison of simulated (upper rows) and acquired (lower rows) undersampled data for three different volunteers (15.', 'DiscussionThe presented results suggest that a radiological analysis can provide reliable age estimates based on hand/wrist MRI using an acquisition time of only 15 seconds, which corresponds to an acceleration factor of approximately 7.']","Figure 5 Comparison of simulated (upper rows) and acquired (lower rows) undersampled data for three different volunteers (15.75, 18.85 and 21.61 years from top to bottom) and locations. Arrows mark structures relevant for age estimation, while circles highlight structures changing their appearance with decreasing acquisition time in both simulated and acquired data.",yes
PMC4610231,Figure_4,oa_package/7c/4e/PMC4610231.tar.gz,['.'],"Fig. 4. Relative mRNA levels of (A), (B), (C), (D), (E), (F), (H) and (I) were determined in the lean and obese rats after a 15-day exposure to vehicle or OLHHA (5mgkg , daily, i.p.). The bars are the meanss.e.m. ( =6-8 determinations per group). The data were analyzed using two-way ANOVA (treatment and genotype) and a test for multiple comparisons. 0.01 and 0.001 denote significant differences compared with the vehicle-treated lean rats; * 0.05 and *** 0.001 denote significant differences compared with the corresponding vehicle-treated group.",yes
PMC7568110,Figure_6,oa_package/0c/62/PMC7568110.tar.gz,"['The histiocytes were diffusely positive for S100 immunostain, consistent with spinal RDD [].', ':(a) Photomicrograph showing large histiocytes with emperipolesis (red arrow) (hematoxylin and eosin stain 400).']",Figure 6: (a) Photomicrograph showing large histiocytes with emperipolesis (red arrow) (hematoxylin and eosin stain 400). (b) The histiocytes show strong diffuse nuclear and cytoplasmic staining for S100 antibody (immunoperoxidase 200).,yes
PMC10087493,Figure_2,oa_package/e9/6e/PMC10087493.tar.gz,[],"FIGURE 2 (A) Lowpower view of the punch biopsy specimen showing normal epidermis with scattered noncaseating granulomas in the superficial dermis. There is also a sparse superficial dermal perivascular lymphocytic infiltrate (H&E, original magnification 40). (B) High power view, of specimen showing granulomas lacking prominent surrounding inflammatory infiltrate, consistent with sarcoidaltype granulomas. There is also a sparse superficial dermal perivascular lymphocytic infiltrate with rare extravasated red blood cells. Vasculitis is not present (H&E, original magnification 100).",yes
PMC8325857,Figure_3,oa_package/8b/74/PMC8325857.tar.gz,"[' 3).', '043aa Test of equality of survival distributions using the Kaplan-Meier Estimator and Log Rank testPrimary tumor was composed mostly of irregular glands, nests of cells, and occasional invasive single cells.', 'The morphology is nearly identical in the primary tumor (A) and metastasis in skin (B) a year after the diagnosisDeath was observed in three GAS patients and six UEA patients.']","Fig. 3 Primary tumor was composed mostly of irregular glands, nests of cells, and occasional invasive single cells. The nuclei varied in size and shape and there was prominent variation from vesicular to highly hyperchromatic nuclei. Nucleoli were present in many cells, but not in all. Occasional goblet cells were also present. Mitotic figures and apoptotic bodies were frequent. Hypocellular and edematous stroma was dominant in many areas of the tumor. The morphology is nearly identical in the primary tumor ( ) and metastasis in skin ( ) a year after the diagnosis",yes
PMC10455750,Figure_1,oa_package/7a/5f/PMC10455750.tar.gz,"['Echocardiographic Abnormalities in the Diagnosis of Cardiac SarcoidosisSeveral TTE abnormalities increase the likelihood of a patient with systemic sarcoidosis having cardiac involvement and should trigger LGE-CMR FDG-PET to confirm the diagnosis (, central illustration).', 'CJ-21-026534176867Central illustration: Prevalence of echocardiographic abnormalities in cardiac sarcoidosis.']",Figure 1 Prevalence of echocardiographic abnormalities in cardiac sarcoidosis. The white arrows indicate multifocal RWMA (top-left image) and global pericardial effusion (bottom-right image). (MR = mitral regurgitation; PHTN = pulmonary hypertension; RWMA = regional wall motion abnormality).,yes
PMC4501135,Figure_10,oa_package/7c/84/PMC4501135.tar.gz,[],Figure 10 Obscuration of underlying tissue by instrument back shadowing is a limitation,yes
PMC6337684,Figure_5,oa_package/cc/ac/PMC6337684.tar.gz,"['Interestingly, the stimulatory effect on SL mRNA expression was markedly enhanced (up to 6 folds basal) especially after 12 24 h of drug treatment with simultaneous exposure to both EGF and NKB (B).', '01 100 nM; C).', '1 1000 nM; D).', 'Synergistic effects of neurokinin B (NKB) with EGF on SL mRNA expression.']","Figure 5 ( , ) Time course of NKB (1 M), and NKB (1 M) + EGF (10 nM) treatment on SL mRNA expression. ( ) Effect of EGF concentration (0.01100 nM) on basal and NKB (1 M)-induced SL mRNA expression in grass carp pituitary cells. ( ) Effect of NKB concentration (0.11000 nM) on basal and EGF (10 nM)-induced SL mRNA expression in grass carp pituitary cells. After drug treatment, total RNA was isolated for real-time PCR of SL mRNA expression. In these experiments, the two-way ANOVA was used to test for significant differences among various groups. * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001. The different capital letters were used to reveal the significant difference between EGF + NKB (1000 nM)-treated group and the control group ( < 0.05). The different lower-case letters were used to present the significant difference between EGF-treated group and the control group ( < 0.05).",yes
PMC4122384,Figure_2,oa_package/e7/6e/PMC4122384.tar.gz,"['HistopathologyIn the intestinal histological sections examined, granular or vacuolar forms of Blastocystis organisms were often observed either within the luminal material or at the tip of the epithelium, but there was no evidence of attachment or invasion of the epithelium ().', 'g002Histological images of Blastocystis organisms in the porcine intestinal biopsies.']",10.1371/journal.pone.0103962.g002,yes
PMC2875667,Figure_5,oa_package/75/7c/PMC2875667.tar.gz,['Bid overexpression highly increases the apoptosis induced in RA FLS by membrane-bound FasL and caspase-9 cleavage after anti-Fas stimulation.'],"Figure 5 . Cultured rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) were transfected as indicated in Figure 4 and treated for 12 hours with 100 ng/ml mFasL or pretreated for one hour with Wortmannine (Wort) or with Wort and the caspase-9 inhibitor, Z-LE(OMe) HD (O Me) FMK (LEHD), before Fas stimulation. Apoptosis, assessed by quantification of nucleosomal release, is shown and data represented the mean (standard error of the mean) of seven RA FLS different lines. Transfected RA FLS were treated for 12 hours with 1 g/ml anti-Fas antibody or pretreated for one hour with Wort before Fas stimulation. Caspase-9 cleavage was analysed by western blot. A representative blot is shown. Densitometric analysis of band intensity of cleaved caspase-9 normalized to full caspase-9 is shown. * indicates < 0.05; # and indicate < 0.05 versus anti-Fas-only treatment.",yes
PMC6599698,Figure_3,oa_package/8c/23/PMC6599698.tar.gz,['Ultrasound examination showing heterogeneous echotexture of the left parotid but without acute inflammatory foci.'],Figure 3 Ultrasound examination showing heterogeneous echotexture of the left parotid but without acute inflammatory foci.,yes
PMC5678288,Figure_4,oa_package/58/27/PMC5678288.tar.gz,"['This region appears brighter in the ex-vivo mean susceptibility maps (yellow circle, ', '']","Fig.4 Increased magnetic susceptibility in the striatum of the rTg4510 (relative to WT) was observed visually in group mean QSMs (a, b (yellow circles)).",yes
PMC8481004,Figure_5,oa_package/b4/58/PMC8481004.tar.gz,"['org/1999/xlink"" xlink:href=""10-1055-s-0041-1735905-i200020-4""/>\nAxial CT venogram image demonstrating a filling defect consistent with thrombus in the left transverse and sigmoid sinuses (arrow).']","Fig. 5 Axial CT venogram image demonstrating a filling defect consistent with thrombus in the left transverse and sigmoid sinuses (arrow). CT, computed tomography.",yes
PMC6112230,Figure_2,oa_package/cd/00/PMC6112230.tar.gz,"['2"">Resection of the Fibular HeadA standard, although somewhat limited lateral, approach to the knee is performed ().', 'Patient is supine with a bump under the operative hip to better position for an approach to the lateral left knee.']",Fig 2 Patient is supine with a bump under the operative hip to better position for an approach to the lateral left knee. A limited lateral approach was completed by centering the incision on the fibular head and placing it at the posterior aspect of the iliotibial band. The peroneal nerve has been identified and protected with a Penrose drain (arrow). This step is critical to avoid iatrogenic injury.,yes
PMC6637505,Figure_10,oa_package/11/87/PMC6637505.tar.gz,[],"Fig. 10 Examples of optic nerve pathology observed in EAE mice 11 and 28days post immunisation. Example of a healthy axonal fibre with normal myelination. Swollen nerve fibres with a fragment of axon left and a large balloon produced from the splitting of the myelin sheaths was observed in EAE mice at 11 dpi. Another large swollen nerve fibre with dark cytoplasm (likely a sign of severe degeneration) filled with numerous vacuoles at 11 dpi. Many bloated myelinated axons were observed at 11 dpi with a dense axoplasm filled with primarily neurofilament. A dystrophic axon where the axoplasm contains an accumulation of lysosomes. Redundant myelination can also be found around the axons at 11 dpi. In EAE mice at 28 dpi, ballooning was also observed in some nerve fibres. Nerve fibres with dark cytoplasm were identified at 28 dpi as well but appeared more shrunken than at 11 dpi. At 28 dpi, large myelinated nerve fibres showed dense degeneration with numerous vacuoles and organelles within the cytoplasm, however they were not as large as fibres at 11 dpi. Empty myelin sheaths with completely degenerated axons were visible at 28 dpi in EAE mice. Dystrophic axons were also found at 28 dpi. Redundant myelin was more common at 28 dpi, often forming around tiny fragments of axons or entirely on its own. v: vacuoles, m: mitochondria, EAE: experimental autoimmune encephalomyelitis. Electron micrograph image resolution: 8nm/pixel",yes
PMC8362796,Figure_1,oa_package/91/68/PMC8362796.tar.gz,['Patient s initial emergency department chest radiograph.'],"Figure 1 Patients initial emergency department chest radiograph. Bilateral airspace consolidations with no acute osseous abnormalities are shown, a consistency found with acute respiratory distress syndrome.",yes
PMC6857259,Figure_1,oa_package/3b/a7/PMC6857259.tar.gz,"[' (A and B)Axial (A), coronal (B) contrast enhanced abdominal CT showing large duodenal varices (white arrows).']","Figure 1 (A and B) Axial (A), coronal (B) contrast enhanced abdominal CT showing large duodenal varices (white arrows). Severe desmoplastic reaction in the region of the superior mesenteric vein, causing complete occlusion (white circle). Superior mesenteric artery patent (black arrow). The black star corresponds to the duodenal lumen",yes
PMC9016609,Figure_1,oa_package/4e/04/PMC9016609.tar.gz,"['It is associated with pulmonary cysts with variable\nshape, wall thickness (s 1\nand 2).', '.', '1177_2050313X221091391-fig1"" position=""float""/>.']",Figure 1. Axial-CT image of the chest (a and b) on lung window shows bilateral septal thickeningin addition to the ground-glass pattern and tree-in-bud opacities.,yes
PMC7575208,Figure_2,oa_package/2c/29/PMC7575208.tar.gz,"[' shows the color variations of H E stained slides in different situations.', 'Examples of images made on the same images but of tissues sections cut a various thickness (2 9 m).']",Figure 2. Examples of images that identify the color difference of stain methods (H&E stain),yes
PMC5672999,Figure_1,oa_package/e1/40/PMC5672999.tar.gz,"['On the right leg, venous disease appeared to have progressed further, presenting as an active ulcer, corresponding to a CEAP classification score of 6 ().', '.', '1177_2050313X17740512-fig1""/>The key pathological feature identified under ultrasound was the presence of thickened vein wall tissue of the deep venous system, located on the proximal popliteal, and the distal femoral vein.']",Figure 1. Picture of right leg showing venous ulceration secondary to occlusion in poplitealfemoral venous segment.,yes
PMC4330237,Figure_13,oa_package/b9/9b/PMC4330237.tar.gz,[],"Fig. 13 A large KCOT has been operated on in the left maxillary sinus. CBCT examination revealed a recurrence distally to d. 27, which was histopathologically proven ( ). Note also the postoperative defect anteriorly and mucosal swelling in the maxillary sinus ( )",yes
PMC8317892,Figure_2,oa_package/64/33/PMC8317892.tar.gz,"['An example of an ERBT specimen is shown in .', 'High-grade pTa papillary urothelial carcinoma.', '3.']","Fig. 2 High-grade pTa papillary urothelial carcinoma. Example of an en bloc resection specimen in which the tumor staging results are accurate. Note that with a wall section of the tumor, the peripheral margin and the deep margin are easily identifiable (hematoxylin and eosin, original magnification 20). The arrow indicates lamina propria and asterisk indicates muscularis propria. DM=deep margin; PM=peripheral margin; T=tumor.",yes
PMC8663547,Figure_3,oa_package/b4/37/PMC8663547.tar.gz,"['In confirmation of our experimental setup, IC-induced NETs stained positive for 8-Oxo-2 -deoxyguanosine (8-OH-dG; a marker of oxidized DNA), TOMM20 (an outer-membrane mitochondrial protein), and citrullinated histone 3 (a marker indicative of the NOX-independent pathway of NETosis), which are all hallmarks of SLE NETosis (16) (Supplemental , A and B).', 'In contrast, resting (unstimulated), healthy neutrophils displayed minimal IL-33 and 8-OH-dG expression, and TOMM20 staining was cytoplasmic (Supplemental A).', 'SLE neutrophils exposed to ICs exhibited increased intracellular IL33 mRNA and protein levels (, A and B), notwithstanding the low IL-33 abundance in myeloid cells (39, 40).', 'Accordingly, both confocal microscopy and immunoblotting in precipitated NETs revealed elevated IL-33 protein levels in IC-released versus spontaneously released SLE NETs (, C and D), whereas SLE ICs, per se, contained no measurable IL-33 (Supplemental A).', 'Furthermore, when comparing NOX-dependent (namely, phorbol 12-myristate 13-acetate treated [PMA-treated] healthy neutrophils) versus NOX-independent (namely, monosodium urate treated [MSU-treated] healthy neutrophils, IC-treated SLE neutrophils) NETs, only the latter exhibited significant IL-33 decoration (Supplemental B), thus suggesting a mechanistic link between NOX-independent pathway of NETosis and IL-33 release.', 'Stimulation of neutrophils, derived from patients with SLE, with nucleic acid-containing ICs led to increased IL-33 expression and IL-33 decoration of NETs.']","Figure 3 Stimulation of neutrophils, derived from patients with SLE, with nucleic acid-containing ICs led to increased IL-33 expression and IL-33 decoration of NETs. ( ) Real-time PCR was performed to confirm differential gene expression of in resting versus IC-treated neutrophils from the peripheral blood of patients with SLE ( = 6). Data were normalized using the average value of resting neutrophils Ct ( Ct minus GAPDH Ct) values. Each dot represents a different donor and bar plots show the mean SEM expression. * < 0.05 (2-tailed, paired test). ( ) Western immunoblotting was performed to examine intracellular IL-33 protein in unstimulated and IC-treated neutrophils from patients with SLE ( = 5). Results were normalized and quantified via densitometry followed by calculation of the relative expression of IL-33 over MPO (loading control). Each dot represents a different donor and bar plots show the mean SEM expression. * < 0.05 (2 tailed, paired test) (left panel). Representative blot ( = 2 experiments) from unstimulated and IC-treated neutrophils obtained from 3 patients (right panel). ( ) Unstimulated or IC-treated SLE neutrophils were cultured for 3 hours and then stained with anticitrullinated histone-3, antiIL-33 (IL-33) antibodies, and DAPI for DNA. Representative confocal images (scale bar: 30 m) in 1 of 3 replicates demonstrate that IC-treated SLE neutrophils generate abundant amounts of IL-33decorated NETs. ( ) Western immunoblotting for IL-33 protein in spontaneously released versus IC-mediated NETs precipitates derived from SLE neutrophils ( = 3).",yes
PMC8943306,Figure_2,oa_package/1c/1d/PMC8943306.tar.gz,"['Vessel sharpness and length for both the Left coronary artery (LCA), which included the left anterior descending artery, and Right coronary artery (RCA) were determined in both phases using coronary reformatting software (SoapBubble) for both 3D CMRAs performed on each patient using both the MVI or 4CH method ( 2).', '\n16\n 2.', '1177_20480040221087556-fig2"" position=""float""/>To assess reproducibility, additional analysis was performed on 25 cardiac MRI studies.']",Figure2. Multiplanar reformatted images from same patient using both techniques. (a) left image and (b) right image are transverse plane reformatted images showing similar image quality of the left coronary artery system utilizing MVI and 4CH techniques on the same patient A:MVI; B:4CH.,yes
PMC2479069,Figure_5,oa_package/a4/ee/PMC2479069.tar.gz,[],(3),yes
PMC6528028,Figure_3,oa_package/37/20/PMC6528028.tar.gz,[':Monomorphic Ventricular Arrhythmias in the Setting of Arrhythmogenic Right Ventricular CardiomyopathyA: Electrocardiographic findings of epsilon waves visualised in lead V1 when the athlete is in normal sinus rhythm; B: Atypical LBBB VT originating from the apical aspect of the right ventricular free wall; C: RAO angiographic view at the time of EP study/RFA demonstrating markedly enlarged RV on angiography (yellow outline); D: Catheter positions during mapping and RFA of VT shown in panel B.'],Figure 3: Monomorphic Ventricular Arrhythmias in the Setting of Arrhythmogenic Right Ventricular Cardiomyopathy,yes
PMC9705270,Figure_7,oa_package/c3/f5/PMC9705270.tar.gz,"['The iliac and ischial osteotomies are connected using a curved, tined osteotome along the medial and lateral portions of the posterior column using an iliac oblique view on fluoroscopy ().', 'Intraoperative fluoroscopic iliac oblique images depicting use of curved, tined osteotome to connect ilium and ischium osteotomies.']","Fig 7 Intraoperative fluoroscopic iliac oblique images depicting use of curved, tined osteotome to connect ilium and ischium osteotomies. The arrows point in the direction of the patients head. These images are taken of the patients left hip.",yes
PMC8517582,Figure_1,oa_package/c1/50/PMC8517582.tar.gz,[],FIGURE 1 (AB) Axial (A) and coronal (B) postcontrast computed tomography (CT) scan of the left temporal bone lesion on presentation; (CD) Axial (C) and coronal (D) postcontrast T1magnetic resonance imaging (MRI) of the lesion on presentation; (EF) Axial (E) and coronal (F) postcontrast T1magnetic resonance imaging (MRI) of the surgical site 10months postoperatively showing fat graft in place and no evidence of recurrent tumor,yes
PMC156027,Figure_2,oa_package/dd/9e/PMC156027.tar.gz,['Photomicrograph showing epithelial ducts seen in a chondromyxoid stroma (Haematoxylin and Eosin 250)DiscussionTumours arising in the minor salivary gland accounts for 22% of all salivary gland neoplasms [1].'],Figure 2 Photomicrograph showing epithelial ducts seen in a chondromyxoid stroma (Haematoxylin and Eosin 250),yes
PMC2193536,Figure_2,oa_package/0e/33/PMC2193536.tar.gz,"['In a second series of experiments, the affect of accessory toxins to the development of inflammation was assessed compared with the ctxAB mutant strain P4 and sham-inoculated controls (Table II and ) .', '.', 'These data are statistically significant when compared with P4 and to other mutants deleted of any single toxin, most notably the rtxA deletion KFV105 (Table II and ).']","Figure 2. Loss of specific cytotoxins affects acute pathology of pulmonary disease. Lung sections from mice intranasally inoculated with P4 and four isogenic mutants were selected for areas demonstrating epithelial lined bronchioles and alveolar air spaces. Note increase numbers of inflammatory cells in the alveoli of the exposed tissue of KFV70 ( ) KFV103 ( ), when compared with the KFV105 ( ), KFV101 ( , , ), and sham-treated samples (H/E, 100).",yes
PMC8615430,Figure_6,oa_package/6b/3d/PMC8615430.tar.gz,"['Effects of DAS/HP- -CD 10% on Histopathology of mdx DIA and GC MusclesThe potential effect of the novel formulation on histopathology was assessed on H E-stained DIA and GC muscle sections, whose representative images are shown in A,C, respectively.', 'As shown in B, DIA muscle of untreated mdx mice showed a significantly higher percentage of total area of damage, and specifically of inflammatory cell infiltrates, in comparison to WT.', 'Results in GC muscle were almost overlapping (D).', 'In (A,C) are shown representative DIA (A) and GC (C) muscle sections stained with hematoxylin and eosin (20 magnification) from mice of each experimental group (WT mice, and mdx mice treated with vehicle VEH or DAS/HP- -CD 10% at the dose of 10 or 20 mg/kg).']","Figure 6 In ( , ) are shown representative DIA ( ) and GC ( ) muscle sections stained with hematoxylin and eosin (20 magnification) from mice of each experimental group (WT mice, and mice treated with vehicleVEHor DAS/HP--CD 10% at the dose of 10 or 20 mg/kg). This staining allows to appreciate the organization of skeletal muscle architecture and its typical alterations in dystrophin-deficient muscles, including the presence of abnormal inflammatory infiltrates or non-muscle (i.e., fibrotic and adipose tissue) areas, quantified via subsequent morphometric analysis, as shown by histograms in ( ) for DIA and ( ) for GC muscle. Values are expressed as mean SEM from the number of mice indicated in brackets. ( ) A statistically significant difference among groups was found by one-way ANOVA for total damage (F = 11, = 0.0002) and infiltration (F = 10, = 0.0003). Bonferroni post hoc test for individual differences between groups is as follows: * vs. WT (0.0002 < < 0.004); N.S. vs. + vehicle ( > 0.05). ( ) A statistically significant difference among groups was found by one-way ANOVA for total damage (F = 6.5, = 0.003) and infiltration (F = 4.2, = 0.02). Bonferroni post hoc test for individual differences between groups is as follows: * vs. WT (0.006 < < 0.04); N.S. vs. + vehicle ( > 0.05).",yes
PMC3519407,Figure_2,oa_package/69/fc/PMC3519407.tar.gz,"['Lesion Characteristics on T2-Weighted Magnetic Resonance ImagingT2-weighted MRI verified the tissue effects of CCI and allowed us to follow the longitudinal development of the brain contusion in vivo ().', 'org/1999/xlink"" xlink:href=""jcbfm2012114f1""/>T2-weighted magnetic resonance imaging (MRI) of a rat brain after controlled cortical impact (CCI).']","Figure 2 T -weighted magnetic resonance imaging (MRI) of a rat brain after controlled cortical impact (CCI). Representative coronal images (bregma 0.5mm) show the development of the cortical contusion from Day 0 (D0, 1 hour after injury) to Day 14 (D14). Tissue disruption was visible early after injury, with ventral shift of the corpus callosum (open arrow, D0) and frequent small intraparenchymal hemorrhages (line arrow, D0). On D1 and D3, ipsilateral cortical edema was visible as a diffuse tissue hyperintensity (open arrow, D1), and brain swelling was indicated by a midline shift (line arrows, D3). By D7 the swelling had subsided, giving way to ipsilateral cortical thinning (line arrows, D7) and ventricular enlargement (open arrow, D7). By D14 a cortical contusion cyst had developed, filled with hyperintense cerebrospinal fluid (line arrow, D14) and/or hypointense blood products (open arrow, D14).",yes
PMC8064403,Figure_3,oa_package/2f/5d/PMC8064403.tar.gz,"['Effect of treatment and cell line was determined using a two-way ANOVA with Tukey s post-hoc test (, 4 and 6E H).', 'Next, we quantified the difference in phosphorylation response following CLM treatment between the human-derived SH-SY5Y cells and mouse-derived N2A cells (A).', ', 2018a) (C, G).', '(2014b) (B, F).', 'Using these two methods, we found that SH-SY5Y cells show a reproducible, dose-dependent and significant increase in both the total amount of p-FUS signal (B) and amount of p-FUS (Ser30) signal present (', 'Therefore, we tested if cytoplasmic FUS increased in N2A cells following CLM treatment (D).', 'SH-SY5Y cells showed a significant increase in the total amount of FUS (E) and p-FUS (', 'In contrast, there was not a significant increase in FUS (E) or p-FUS (', 'We used a DNA-PK antibody that can detect both human and mouse DNA-PK species (Supplemental ).', '.']","Fig. 3. FUS is not phosphorylated or re-localized to the cytoplasm in mouse cells following CLM treatment. Human derived SH-SY5Y cells and mouse derived N2A cells were directly compared by western blot. (A) SH-SY5Y and N2A cells were treated with increasing doses of CLM for 2 h. Following treatment, RIPA extracted whole cell lysate was analyzed using the following antibodies: FUS, p-FUS (Ser30), and p-H2AX. (B) Quantification of (A) for phosphorylation of FUS (top band) at different concentrations of CLM were normalized to total protein. (C) Quantification of (A) for phosphorylation of FUS at residue Ser30 at different concentrations of CLM were normalized to total protein. Error bars indicate mean SEM ( = 3). (D) SH-SY5Y and N2A cells were treated with increasing doses of CLM for 2 h. Cytoplasmic and nuclear fractions were collected and analyzed by western blot using the following antibodies: FUS, p-FUS (Ser30), GAPDH and H3. GAPDH and H3 were used as markers for cytoplasmic and nuclear fractions, respectively. (E) Quantification of (D) for the percentage of FUS found within the cytoplasmic fraction was normalized to total protein. (F) Quantification of (D) showing phosphorylation of FUS (top band) at different concentrations of CLM was normalized to total protein. (G) Quantification of (D) for phosphorylation of FUS at residue Ser30 at different concentrations of CLM was normalized to total protein. Error bars indicate mean SEM ( = 5). All control cells (0) received DMSO for 2 h.",yes
PMC4747167,Figure_6,oa_package/7b/f0/PMC4747167.tar.gz,"['Analysis the effect of autophagy inhibition or induction on the effects of alcohol and oleate on lipid accumulation, lipid peroxidation and inflammationHepG2 E47 cells were pre-incubated with 0.']","Figure 6 Analysis the effect of autophagy inhibition or induction on the effects of alcohol and oleate on lipid accumulation, lipid peroxidation and inflammation HepG2 E47 cells were pre-incubated with 0.2mM oleate (Ol) or BSA (served as control) for 24h. Subsequently, cells were co-incubated with 3-methyl adenine (3-MA), an autophagy inhibitor (2.5mM) or rapamycin (0.2g/ml) for 1 h before adding 50mM alcohol (Alc) to cultured medium for additional 24 h. , Cellular triglyceride content normalized to total cellular protein. , Analysis of cellular MDA levels by TBARS assay. , Analysis of ICAM-1 mRNA levels by quantitative RT-PCR analysis. (*: < 0.05 compared to corresponding control; #: < 0.05 compared to corresponding oleate or alcohol condition; $: < 0.05 compared to the equal condition in control group).",yes
PMC7533517,Figure_7,oa_package/10/d8/PMC7533517.tar.gz,"['05; A-C).', '.']","Figure 7. TDI-induced redistribution of -SMA and HMGB1 in 16HBE cells. MK2206 and DEX attenuated redistribution of (A) -SMA and (B) HMGB1, as analyzed via immunofluorescence and (C) quantified. *P<0.05 vs. AOO group; P<0.05 vs. TDI group. TDI, toluene diisocyanate; AOO, acetone and olive oil; DEX, dexamethasone; -SMA, -smooth muscle actin; HMGB1, high mobility group box 1.",yes
PMC11595077,Figure_2,oa_package/19/28/PMC11595077.tar.gz,"['Immunohistochemistry was negative for smooth muscle actin, desmin, and myogenic markers (myogenin and MyoD1), but demonstrated diffuse positive nuclear staining for -catenin (), which is supportive of SNM with WNT/ -catenin pathway dysregulation.', '(A) H E 4 a low power image showing loose myxoid stroma with spindle cells, and a peripheral rim of reactive new bone formation which was present around portions of the lesion.']","Figure 2 ( ) H&E 4a low power image showing loose myxoid stroma with spindle cells, and a peripheral rim of reactive new bone formation which was present around portions of the lesion. ( ) H&E 20a higher power image demonstrating bland spindle and stellate cells with a tissue-culture-like appearance similar to that seen in fasciitis-like lesions. ( ) Beta-catenin immunohistochemistry (from the initial biopsy sample) confirming diffuse nuclear expression (with weak cytoplasmic staining), indicating alteration of the WNT-pathway.",yes
PMC8465219,Figure_1,oa_package/f4/94/PMC8465219.tar.gz,"['Other components are specific to the cell of origin, as shown in .', 'Acknowledgments was constructed using the BioRender.', '1007/s11912-017-0568-728229393Biogenesis of prostatic exosomes.']","Figure 2 Barcode plot figure [ ] showing significantly higher enrichments in exosomes compared to cells associated with immune response (n = 236 transcripts), apoptosis and DNA repair (n = 200), and prostate cancer (n = 262). The barcode plot shows the log2 fold change in the X-axis. The Y-axis is composed by score (-log10 false discovery rate or adjusted values) and enrichment (gene set-weighted density estimation). The middle bar and colored histogram in either top or bottom of the bar reflect the number of differentially expressed genes (adjusted values < 0.05). The bottom section of the figure shows the standardized expression profiles of genes associated to the pathways showed in the top section. The data in this figure are available through Dogra et al. [ ], representing the comparison between patient tissues and serum-extracted exosomes.",yes
PMC6634282,Figure_1,oa_package/41/25/PMC6634282.tar.gz,[' Invasive angiogram demonstrating anomalous right coronary artery (arrow) Invasive angiogram demonstrating anomalous right coronary artery (arrow)DiscussionAn anomalous origin of the right coronary artery (RCA) is a rare congenital anomaly that was first described in 1948 by White and Edwards [4].'],Figure 1 Invasive angiogram demonstrating anomalous right coronary artery (arrow),yes
PMC9332618,Figure_1,oa_package/ff/fa/PMC9332618.tar.gz,"['For , only a normal distribution was observed for the data in B.', 'The data from C F showed a non-normal distribution, so non-parametric Mann-Whitney tests were performed.', 'A used a two-way ANOVA with a post hoc d k s multiple comparisons test.', 'Therefore, we measured weight loss and viral load by quantitative RT-PCR from the lungs of mice at 10 dpi and used as positive controls (C+) the viral load at 3 dpi (A,B).', 'No significant differences were found in the weight loss after the infection compared to mock-treated mice (A).', 'As shown in B, animals showed levels of n-hRSV RNA close to non-detectable, indicating an adequate viral clearance by day 10 post-challenge, making it an appropriate inoculation time point for subsequent BCG challenge.', 'These results correlated with a low percentage of neutrophils recruited in the lungs (C).', 'However, no differences were observed in the transcription factor controlling ho-1 gene expression nrf2 between the groups (F).', 'The absence of complete recovery in the pulmonary tissue can be suggested due to the significant increase of CD200 (F) and HO-1 relative expression (E), which promote an anti-inflammatory environment [55].', '01326117320Evaluation of infection, inflammation, and immunomodulatory parameters from human respiratory syncytial virus (hRSV)-infected mice.']","Figure 1 Evaluation of infection, inflammation, and immunomodulatory parameters from human respiratory syncytial virus (hRSV)-infected mice. ( ) Body mass loss of C57BL/6 mice infected with 1 10 plaque formation units (PFU) of hRSV A2 for ten days. All the following parameters were measured at day 10 post hRSV infection. ( ) Determination of viral load through specific real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) for hRSV. ( ) Neutrophils ( ) Heme Oxygenase (HO)-1, ( ) OX-2 glycoprotein membrane (CD200), and ( ) nuclear factor erythroid 2-related factor (NRF2). Data are shown as median interquartile range of at least two independent experiments with three animals per group. ( ) One-way ANOVA was performed with a post-hoc Tukey test. ( ) t-student was performed with the Mann-Whitney U test (* < 0.05; *** 0.001). Created with BioRender.com.",yes
PMC4263805,Figure_2,oa_package/b3/45/PMC4263805.tar.gz,"[' 2 and 3).', 'No axillary lymph node uptake was seen (not shown)\nA 67-year-old woman with a history of marginal zone B-cell lymphoma (mucosa associated lymphoid tissue [MALT] lymphoma) presents with screening mammogram detected new mass in the right breast.', ' 2 and 4).']","Fig. 2 A 74-year-old woman with primary large B cell breast lymphoma presenting with a palpable mass in the left breast. Cranio-caudal left breast mammogram shows an isodense circumscribed mass ( ) in the medial left breast. Ultrasound of the palpable mass demonstrates an oval, circumscribed principally echogenic mass with an irregular shaped central area of hypoechogenicity ( ). PET/CT shows two areas of homogeneous hypermetabolic uptake in the medial left breast. No axillary lymph node uptake was seen ( )",yes
PMC11469643,Figure_3,oa_package/16/2a/PMC11469643.tar.gz,"['Pathology () reported a calcified tissue with spindle cell proliferation without neoplasic features, and complementary immunohistochemistry of the calcified material was positive for enolase and D240 and negative for inhibin and Olig 2.', 'Hematoxylin eosin (HE) staining with extensive calcified tissue is recognized.']","Figure 3 Hematoxylineosin (HE) staining with extensive calcified tissue is recognized. There are areas of myxoid tissue, focal piloid proliferation, arachnoid cellularity, and mononuclear inflammatory cells.",yes
PMC10614774,Figure_3,oa_package/b5/ca/PMC10614774.tar.gz,"['As shown in (A), we observed a significant increase in hyperplasia in the medial compartment of PMX operated mice compared to sham mice.', 'Increases in subsynovial cellularity were observed in all four anatomic locations in this model, although with more pronounced changes on the medial side of the knee (B).', '562259v1-f0002"" position=""float""/>:(A-C) Histopathologic scoring of synovial hyperplasia, cellularity and fibrosis respectively for medial tibial (MT), medial femoral (MF), lateral tibial (LT) and lateral femoral (LF) areas in mice 12 weeks after PMX or sham surgery (n=5/group).']","Figure 3: (A-C) Histopathologic scoring of synovial hyperplasia, cellularity and fibrosis respectively for medial tibial (MT), medial femoral (MF), lateral tibial (LT) and lateral femoral (LF) areas in mice 12 weeks after PMX or sham surgery (n=5/group). Each score represents the average score of 4 blinded scorers. Multiple unpaired Mann-Whitney tests corrected with Holm-idk test. Mean 95% CI.",yes
PMC6305935,Figure_4,oa_package/29/58/PMC6305935.tar.gz,"['Red: left right, green: anterior posterior, blue: superior inferiorStructural connectivity correlated with tau deposition in temporal lobe.']","Figure 4 Structural connectivity correlated with tau deposition in temporal lobe. The CN group shows increased connectivity (FDR=0.0023), whereas the ADspectrum group shows decreased connectivity (FDR=0.015). Red: leftright, green: anteriorposterior, blue: superiorinferior",yes
PMC7709664,Figure_2,oa_package/b2/38/PMC7709664.tar.gz,"['Finally, no differences were found between pathological groups (Table 4 and Table 5, ).', 'Pairwise comparison between group levels for relative strength.']",Figure 2 Pairwise comparison between group levels for relative strength. ( : knee strength for men; ( : ankle strength for men; ( : knee strength for women; ( : ankle strength for women. Abbreviation: *: < 0.05; **: < 0.01; ***: < 0.001; ****: < 0.0001; HEG: healthy group; KTR: kidney transplant recipient group; LTR: liver transplant recipient group; OB: elderly with obesity group; ISOK: isokinetic muscular strength; ISOM: isometric muscular strength; EXT: extension; FLEX: flexion; R: right; L: left.,yes
PMC7135851,Figure_1,oa_package/ee/f6/PMC7135851.tar.gz,"['4) using solid-phase enzyme-linked\nimmunosorbent assays (ELISA) (s 1A and Having verified the 2m collagen I interaction\nunder physiological pH conditions, we next monitored amyloid growth\nof 2m in the presence or absence of collagen I by\nthioflavin T (ThT) fluorescence (B).', '4 (B, blue), as evident\nby enhanced ThT fluorescence.', 'This is not observed in the absence\nof collagen I in the same conditions, and collagen I alone does not\nshow ThT fluorescence enhancement (B).', '21 Atomic force\nmicroscopy (AFM) images also showed that 2m interacts\nwith collagen I fibrils, consistent with previous results,21 showing that 2m coats the\ncollagen I fibril surface before detectable fibril formation occurs\n(monitored by ThT fluorescence), obscuring the characteristic collagen\nI fibril D-banding that is clearly observed in the absence of 2m (C,D).', 'Importantly, control experiments showed that 2m alone (E)\ndoes not aggregate in the conditions employed, with no fibrils or\nhigh molecular weight assemblies observed by AFM.']","Figure 1 Detection of collagen I-driven m amyloid formation.(A) ELISA probing dose-dependent adhesion of m(1080 g/mL) to collagen I (10 g/mL) or casein(10 g/mL, used as a negative control), at pH 7.4. The averageabsorbance at 450 nm from triplicates within the same plate are reportedwith the standard deviation given as error bars. (B) ThT fluorescencecurves of 85 M m (black), 85 M m + 3.4 mg/mL (8.5 M) collagen I (blue), or 3.4 mg/mLcollagen I alone (orange) over 650 h in 10 mM sodium phosphate buffer,pH 7.4, shaking at 600 rpm at 37 C. Three representative curvesare given for each condition. The inset shows a zoom-in of the baselineof the ThT fluorescence curves to highlight the lack of fluorescenceenhancement for both m (black) and collagen I (orange)alone. (CE) Representative amplitude-modulated AFM imagesof (C) m coincubated with collagen I fibrils, (D)collagen I fibrils alone, and (E) m alone afterincubation for 96 h at 37 C with shaking.",yes
PMC11041591,Figure_5,oa_package/09/d7/PMC11041591.tar.gz,"['Pathologists identified areas of distinct tissue type with sufficient cellularity for sequencing (online supplemental figure 5), which were macrodissected and processed as described in the online supplemental methods.', 'In the WGS of 14 GM-only samples and 27 IM-only samples, a significantly lower mutational burden in GM compared with IM was evident, even when the samples were partitioned into short and long segments (figure 5A).', 'The difference in burden was also not explained by the ages in the two groups (figure 5B).', 'SBS17a/b were detected in nearly all IM samples, but only in one GM sample (figure 5C).', 'GM does not bear the genomic hallmarks of OAC.', 'By contrast, 16 IM samples (59%) had an alteration in at least one early IM/OAC gene (figure 5D).', 'There was no difference in SCA load between GM and IM non-progressors (figure 5E), and both were an order of magnitude lower than in OAC samples (online supplemental figure 3).']","Figure 5 GM does not bear the genomic hallmarks of OAC. (A) Mutational burden by sample type and BO segment length. (B) Mutational burden by age. (C) SBS signature contribution by sample. (D) Mutations in OAC associated genes, split by genes typically mutated early and late in the progression of OAC. Only genes with mutations in this cohort, along with TP53, are shown. (E) SCA load by sample, taken as the length of genome altered by a copy gain, loss or copy neutral loss of heterozygosity. BO, Barretts oesophagus; GM, gastric metaplasia; IM, intestinal metaplasia; OAC, oesophageal adenocarcinoma; SCA, somatic chromosomal alterations; SNV, single nucleotide variant.",yes
PMC5991933,Figure_3,oa_package/63/ab/PMC5991933.tar.gz,['Scottie dog x-rayScottie dog and x-ray showing resemblance.'],Figure 3 Scottie dog x-ray Scottie dog and x-ray showing resemblance.,yes
PMC2841085,Figure_1,oa_package/ac/3e/PMC2841085.tar.gz,['Typical full body DXA scan.'],Figure 1 . The android region of interest (ROI) is highlighted in blue and the gynoid ROI is highlighted in red (refer to text for the landmarks that define these regions).,yes
PMC11365710,Figure_1,oa_package/2b/04/PMC11365710.tar.gz,"[""Hyperpigmented macules on the patient's buccal mucosaCT scan of the abdomen and pelvis with intravenous and rectal contrast was performed which revealed jejuno-jejunal intussusception containing several polyps (the largest measured 32 mm) with mildly dilated proximal jejunum ().""]",Figure 1 Hyperpigmented macules on the patient's buccal mucosa,yes
PMC8590575,Figure_1,oa_package/54/bf/PMC8590575.tar.gz,"['\nAbdominal ultrasound (US) was performed, which showed a heterogeneous thickening of the gallbladder fundus wall with low resistance flow on power Doppler and hypoechoic intramural nodules (\n\n).', '\n(\nA-D\n) Ultrasound.']",Fig. 1 ( ) Ultrasound. A heterogeneous thickened gallbladder wall is shown with low resistance flow on power Doppler and hypoechoic intramural nodules (arrows).,yes
PMC5102431,Figure_2,oa_package/f6/a0/PMC5102431.tar.gz,"['In contrast to M-CSF (30 ng/ml) and CXCL4 (10 g/ml), CXCL4L1 (1 and 10 g/ml) did not stimulate monocyte survival ().', 'Microscopic analysis demonstrated that, after 6 days in culture, monocytes stimulated with 30 ng/ml M-CSF or with 10 g/ml CXCL4 showed a normal cell density and a morphology characteristic of macrophages, whereas CD14+ monocytes stimulated with CXCL4L1 (1 and 10 g/ml) were far less numerous and their numbers were comparable to those of unstimulated monocytes (A and data not shown).', '7%) (B).', 'When the amount of intracellular ATP, which is a good parameter for cell viability and metabolic activity and thus the cell number in cultures, was quantified using an ATP assay kit, the number of macrophages after stimulation with CXCL4L1 (1 and 10 g/ml) or 1 g/ml CXCL4 was significantly lower compared to cell numbers in cultures treated with 10 g/ml CXCL4 or 30 ng/ml M-CSF (C).', 'Moreover, CXCL4L1 (10 g/ml) statistically inhibited macrophage survival induced by CXCL4 (10 g/ml) (D).', 'g002In contrast to CXCL4L1, CXCL4 induces morphological changes in monocyte-derived macrophages and promotes their survival.']",10.1371/journal.pone.0166006.g002,yes
PMC10520382,Figure_22,oa_package/39/3a/PMC10520382.tar.gz,[],"Figure 22 Newly-formed small blood vessels surrounding osseous trabeculae. Anti-CD34 antibody immunomarking, 200.",yes
PMC2978115,Figure_3,oa_package/31/64/PMC2978115.tar.gz,['Radiology of hind legs in adjuvant induced arthritic rats.'],Figure 3 .,yes
PMC8636044,Figure_1,oa_package/76/b2/PMC8636044.tar.gz,"['Microcontact PrintingThe silicon wafer master mold (A) was a kind gift from Jenny Emn us and Janko Kajtaz (Department of Biotechnology and Biomedicine, DTU Bioengineering, Technical University of Denmark), and has been used previously in a common publication (1).', 'Characterization of microcontact printing ( CP).', 'Using the wafer master mold (A), PDMS stamps (B) were produced with a size of 2 8 mm (s 1C,D) and 28 lanes (D).', 'The printing of an anti-goat Alexa-546 antibody showed several red lines under the fluorescence microscope (E), with a line width of 30 m and a space of 30 m (F).', 'The staining was not seen in the green channel, showing the specificity of CP (G).', ', we can microprint a 50 kDa tau protein but also a 4 kDa -amyloid peptide (Supplementary ).', 'The method of CP in combination with organotypic brain slices is very potent and promising and has several future applications: (a) First, principally we can microprint any protein or peptide of interest (in preliminary experiments we microprinted tau and A , Supplementary ).']","Figure 1 Characterization of microcontact printing (CP). The silicon wafer master mold with 6 5 stamp masters. Two polydimethylsiloxanes (PDMS) stamps. In the PDMS stamp is seen under a phase-contrast microscope. A typical stamp has a length of 8 mm and a width of 2 mm including 28 lanes . The microcontact printing was set up with the printing of an anti-goat Alexa-546 antibody showing red lines with 30 m size and space , which is not seen in the green channel . Scale bar in A = 1.75 cm , 0.5 cm , 250 m , 140 m , 52 m .",yes
PMC7605764,Figure_5,oa_package/dd/6c/PMC7605764.tar.gz,['.'],Fig. 5. ( ) Coronal cross section of the 3D model illustrating coverage angle calculation. The total coverage angle is the angle between the line connecting the center of the best-fit spheres for each pelvis (identified by the two green dots) and the edge of the acetabulum. The coverage angle for the fossa is the line connecting the center of the best-fit spheres and the edge of the fossa (identified as the red area). The weight-bearing coverage area is the difference between the two (WB = weight-bearing right/left and = Fossa right/left). ( ) Diagram of the five clinically pertinent coverage angle octants of the acetabulum. The coverage angle reported for the octant section is the average of the coverage angles within the specified 45 (or octant) of the acetabulum edge.,yes
PMC7571907,Figure_4,oa_package/ef/2a/PMC7571907.tar.gz,['Abdominal CT scan.'],"Figure 4 Abdominal CT scan. (A) Non-contrast images showing venous calcification (white arrow) form the ascending colon to the hepatic flexure. (B) Coronal reformatted T images in the venous phase showing wall thickening limited to the ascending colon, and the scope of vein calcification is larger than that of colon wall swelling and thickening.",yes
PMC8281787,Figure_3,oa_package/1b/5a/PMC8281787.tar.gz,"['Axial ADC, left, and axial DWI, right, both at the level of the parotids.']","Figure 3 Axial ADC, left, and axial DWI, right, both at the level of the parotids. The ADC map shows a very low signal with a corresponding high signal on DWI of the left parotid mass (white arrows). This is consistent with significantly restricted diffusion. ADC:apparent diffusion coefficient; DWI:diffusion-weighted imaging",yes
PMC2770122,Figure_1,oa_package/9d/82/PMC2770122.tar.gz,"['The total area-under-curve was calculated for IgG1 and IgG2 responses at 0, 4 and 21 mpe, and plotted separately for adjuvant-control animals, vaccinated cattle that became patent, and vaccinated animals that were protected from patent infection (figure 1).', 'The only marked difference in area-under-curve between patent and non-patent vaccinated animals was observed with the IgG2 response to OoTMY1 (figure 1), with a higher median level exhibited by protected cattle (Mann-Whitney U test, P = .', 'g001Total area-under-curve (OD405 nm) for IgG1 and IgG2 responses to eight Onchocerca ochengi recombinant antigens.']",10.1371/journal.pntd.0000544.g001,yes
PMC3847372,Figure_5,oa_package/f5/2d/PMC3847372.tar.gz,['Cloning and sequencing of novel ZNF695 transcripts associated to ovarian cancer.'],"Figure 5 PCR product of ZNF695 showing the three different RNA transcript sizes of ZNF695 expressed in malignant ovary tissue sample 6 (MOT6, right) and the individual amplicons used for cloning, with the size (in base pairs) shown on the left. Ladder (line 1), ZNF695 splice variants 1 and/or 2 (lane 2, 400bp), ZNF695 splice variant 4 (lane 3, 360bp), ZNF695 splice variant 5 (lane 4, 310bp), and ZNF695 amplicons in MOT6 (line 5). Sanger sequence plot of ZNF695 splice variant 1 and 2 at the boundary of exons 1 and 2. Sanger sequence plot of ZNF695 splice variant 1 and 2 at the boundary of exons 2 and 3. Sanger sequence plot of ZNF695 splice 5. This transcript has alternative splicing in exon 12, with the arrow illustrating the alternative splicing site at the exon 12 boundary. Sanger sequence plot of ZNF695 splice variant 4. This sequence has alternative site splicing in exon 23 (arrows), this sequence are contain in ZNF695 variant 5 too. AS model of ZNF695 gene. Black lines represent primary splicing of exons 1-2-3 of ZNF695 gene containing the full coding sequence that corresponds to the largest amplicon. Green dotted line shows exons 23 AS, which corresponds to the intermediate amplicon. Red dotted line shows exon 1 AS together with exons 23 AS, which are contained in the smaller amplicon.",yes
PMC10746493,Figure_3,oa_package/91/54/PMC10746493.tar.gz,"['Computerized Tomography Coronary Angiogram(A) Aneurysm of the saphenous vein graft; (B) Stent within the aneurysmDue to the size of the aneurysm, the patient was transferred to our facility for evaluation by the cardiothoracic (CT) surgery team.']",Figure 3 Computerized Tomography Coronary Angiogram (A) Aneurysm of the saphenous vein graft; (B) Stent within the aneurysm,yes
PMC10777259,Figure_1,oa_package/fb/fd/PMC10777259.tar.gz,"[' Erythematous and hyperpigmented plaque on the right calf, exhibiting oedema, bullae, and erosions.']","Figure 1 Erythematous and hyperpigmented plaque on the right calf, exhibiting oedema, bullae, and erosions.",yes
PMC9792328,Figure_1,oa_package/89/b5/PMC9792328.tar.gz,['Blood Parasite Smear.'],"Figure 1 Blood Parasite Smear. Intraerythrocytic rings. Parasitemia of 3.6%. Prepared as thin prep slides stained with traditional Wright-Giemsa stain at 100x. Imaging spliced to highlight relevant findings, with dashed line to denote cutoffs.",yes
PMC11370371,Figure_1,oa_package/27/be/PMC11370371.tar.gz,"['50 52 To investigate the induction and regulation of astrocytic ROS in response to pathogenic stimuli, we first measured the rates of cellular H2O2 efflux in primary mouse astrocytes (Extended Data a,b) upon stimulation with neuroimmune factors induced in neurological disease.', '53 58 The cytokines interleukin-6 (IL-6) and interferon- (IFN- ) had no effects on H2O2 efflux, whereas a cocktail of cytokines linked to astrocyte-dependent neurotoxicity, consisting of IL-1 , tumor necrosis factor- (TNF ), and complement component 1q (C1q),53 increased the rates of astrocytic H2O2 production by 24 h (a).', 'The cocktail-induced H2O2 production was attributable to IL-1 , but not to TNF or C1q (a), suggesting that astrocytic ROS is engaged by specific types of ligands and signaling mechanisms.', 'To test the contribution of CIII-ROS in astrocyte H2O2 efflux, we treated astrocytes with IL-1 and one of two structurally distinct S3QELs (b) to rule out common off-target effects.', '3%) (c), suggesting that CIII is the dominant source of IL-1 -induced increases in H2O2 levels.', 'As reported in other cell types,7,12,13,59 S3QELs and S1QELs did not affect mitochondrial metabolism (Extended Data c,d).', 'To further investigate the mechanisms regulating CIII-ROS, we targeted the highly sensitive and selective ratiometric H2O2 sensor HyPer760 to mitochondria (mtHyPer7; d and Extended Data ', '2 (Extended Data g,h), indicating that mtHyPer7 enables precise and spatially resolved measurements of astrocytic CIII-ROS.', '2 reduced baseline mitochondrial H2O2 levels (e), suggesting that CI-ROS plays a greater role than CIII-ROS in setting the basal redox tone in astrocyte mitochondria, in line with previous reports.', 'Mitochondrial H2O2 was increased within 4 h, peaked by 6 h, and remained elevated until at least 8 h (f).', '49% (e,g).', '2 and the NOX-ROS inhibitor APX-115 had no effects (e and Extended Data ', '22,63 66 Indeed, we found that oA caused dynamic changes in astrocytic mtROS levels, increasing mtROS within 2 h, which remained elevated until 3 h after stimulation (h).', '2 fully blocked this induction (i,j), revealing a pronounced role of CIII in oA -induced mtROS responses.', 'Unlike IL-1 and oA , stimulation with IL-6 did not increase astrocytic mtROS across multiple timepoints (Extended Data j), confirming its lack of effect on cellular H2O2 efflux (', 'However, since blockade of astrocytic CIII-ROS and not CI-ROS affects cytokine-induced mtROS in astrocytes (e), ectopic expression of AOX functionally complements the pharmacological suppression of CIII-ROS during cell stimulation.', 'Although IL-1 substantially increased astrocytic mtROS levels (as shown in ), we did not detect global increases in oxidation states in the cysteine proteome.', '2,3,12 Although S3QEL alone did not change basal mtHyPer7 oxidation (e), S3QEL did alter a small subset of basal transcripts (Extended Data ', '1186/s13059-014-0550-825516281\n.']","Figure 1. Astrocytic CIII-ROS are induced by specific stimuli. , Cellular H O efflux after 24-h treatment with vehicle, IL-1 (3 ng/mL), TNF (30 ng/mL), C1q (400 ng/mL), IL-6 (33 ng/mL), IFN- (10 ng/mL) with or without S3QEL2 (20 M) or S3QEL1.2 (1 M). n=612 wells, ANOVA with Dunnetts ( ) or Tukeys test ( ). , Schematic of mtHyPer7 sensing mitochondrial CIII-derived H O . , Measurement of mtHyPer7 fluorescence 6 h after treatment with vehicle, IL-1, S3QEL1.2 (3 M), or S1QEL2.2 (10 M). n=162297 cells, Kruskal Wallace with Dunns test. , Time-course of mtHyPer7 fluorescence after vehicle or IL-1. n=56296 cells, Mann-Whitney, unpaired two-tailed t-test. , Representative images of mtHyPer7 oxidized/reduced ratios after 6-h treatment with vehicle, IL-1, or IL-1 with S3QEL1.2. Scale bars: 10 m; 5 m (inset). , Time-course of mtHyPer7 fluorescence after vehicle or oA (300 nM). n=54310 cells, Mann-Whitney, unpaired two-tailed t-test. , Quantification of mtHyPer7 after 3-h treatment with vehicle, oA, or S3QEL1.2 (3 M). n=110233 cells, Kruskal Wallace with Dunns test. , Representative images of mtHyPer7 oxidized/reduced ratios after 3-h treatment with vehicle, oA, or oA with S3QEL1.2. Scale bars: 10 m, 5 m (inset). Data are shown as mean SEM. *p<0.05, **p<0.01, ***p<0.001.",yes
PMC4936994,Figure_1,oa_package/a0/2f/PMC4936994.tar.gz,"[' 1).', '', 'b, c The density is uniform, and the enhancement degree of the tumor was significantly lower than that of the surrounding normal renal parenchymaTreatmentTumors of all 6 cases were removed laparoscopically (RLPN) via an retroperitoneal approach.']","Fig.1 Preoperative CT scan. Non-contrast CT scan results indicated that there was soft tissue density in the lower-middle portion of the kidney, with a clear boundary, a size of 4.23.8cm and a CT value of 3036 HU. , The density is uniform, and the enhancement degree of the tumor was significantly lower than that of the surrounding normal renal parenchyma",yes
PMC9807485,Figure_5,oa_package/ab/3f/PMC9807485.tar.gz,"[' 5 and 6).', 'Table 3Distribution of TTR amyloid depositionThe percentage was calculated according to the number of cases with amyloid deposition in each region (binary information present/absent)*Basal ganglia refers to subependymal vesselsSchematic representation of stages in the distribution of TTR amyloid deposition in a medial view (upper figure) and in a coronal section at level of the basal ganglia of the human brain (lower figure).', 'The amyloid deposition is represented by the colored dotsExamples of CNS TTR stages.']",Fig. 5 Schematic representation of stages in the distribution of TTR amyloid deposition in a medial view (upper figure) and in a coronal section at level of the basal ganglia of the human brain (lower figure). The amyloid deposition is represented by the colored dots,yes
PMC6027800,Figure_1,oa_package/e4/dc/PMC6027800.tar.gz,"['On admission, the patient underwent magnetic resonance angiography of cervical arteries () which confirmed the findings of color Duplex scan, and magnetic resonance imaging (MRI) of the brain that did not reveal any lesions.', '1007/BF020018948792997Magnetic resonance angiography showing (A) a right internal carotid artery occlusion (red arrow), a patent right external carotid artery (green arrow), a patent right common carotid artery (white arrow) and a patent right vertebral artery (blue arrow); (B) a left common carotid artery occlusion (white arrow), a patent left external carotid artery (green arrow), a patent left internal carotid artery (red arrow) and a patent distended left vertebral artery (blue arrow).']","Fig. 1 Magnetic resonance angiography showing (A) a right internal carotid artery occlusion (red arrow), a patent right external carotid artery (green arrow), a patent right common carotid artery (white arrow) and a patent right vertebral artery (blue arrow); (B) a left common carotid artery occlusion (white arrow), a patent left external carotid artery (green arrow), a patent left internal carotid artery (red arrow) and a patent distended left vertebral artery (blue arrow).",yes
PMC6045835,Figure_10,oa_package/85/a2/PMC6045835.tar.gz,[],Fig. 10 LC-MS chromatogram. Identification of decursin in Jawoongo. Mass spectrum peak at 53.028min,yes
PMC9536207,Figure_3,oa_package/ec/74/PMC9536207.tar.gz,"['19% (n = 50) on the bilateral tentorium leaf () (Table 2).', 'C) On sagittal T2-WI, the gyrus, which is herniated into the quadrigeminal cistern (arrow), is observedMagnetic resonance images of a 52-year-old female patient with a headache.']",Figure 3 Magnetic resonance images of a 52-year-old female patient with a headache. ) Axial T2-WI shows bilateral gyri herniation into the quadrigeminal cistern (arrows) due to hypoplasia in both leaves of the tentorium cerebelli. ) Herniations of the gyri to the quadrigeminal cistern (arrows) and partial compression effect on the internal cerebral veins (arrowhead) are remarkable on coronal T2-WI. ) The size of the herniation is seen more clearly on sagittal T2-WI,yes
PMC5005001,Figure_1,oa_package/de/eb/PMC5005001.tar.gz,"['The right eyeball was displaced anteriorly and inferiorly ().', '12 However, in our patient s CT images, the orbital neoplasm was an isolated homogenous mass occupying the upper inner position of the right orbit, which does not support the possibility of direct invasion ().', 'References1KiyotaNTaharaMFujiiSNonplatinum-based chemotherapy with irinotecan plus docetaxel for advanced or metastatic olfactory neuroblastomaCancer20081124885891181892942ThompsonLDOlfactory neuroblastomaHead Neck Pathol200933252259205969813MoritaAEbersoldMJOlsenKDFooteRLLewisJEQuastLMEsthesioneuroblastoma: prognosis and managementNeurosurgery199332570671584928454AnsariSAhmadKDhungelKGuptaMKAmanullahMFEsthesioneuroblastoma: one of the causes of proptosisHead Face Med2013919238900745ChanLPWangLFTaiCFWuCCKuoWRHuge sphenoid sinus olfactory neuroblastoma: a case reportKaohsiung J Med Sci20092528792193214126KamathVBSowmyaVBallalCKMendoncaNEsthesioneuroblastoma as an unusual cause for dystopiaOrbit2013326392394240634117LopezRMazzoniLChaputBJalbertFOlfactory neuroblastoma presenting with exclusive orbital manifestationsJ Craniofac Surg2013242667669235247738LaforestCSelvaDCromptonJLeibovitchIOrbital invasion by esthesioneuroblastomaOphthal Plast Reconstr Surg20052164354409BobeleGBSexauerCBarnesPAKrousHFBodensteinerJBEsthesioneuroblastoma presenting as an orbital mass in a young childMed Pediatr Oncol1994224269273810765910DulguerovPAllalASCalcaterraTCEsthesioneuroblastoma: a meta-analysis and reviewLancet Oncol20012116836901190253911BroichGPagliariAOttavianiFEsthesioneuroblastoma: a general review of the cases published since the discovery of the tumour in 1924Anticancer Res1997174A26832706925270112YuTXuYKLiLEsthesioneuroblastoma methods of intracranial extension: CT and MR imaging findingsNeuroradiology200951128418501966973913MoriRSakaiHKatoMOlfactory neuroblastoma with spinal metastasis: case reportNo Shinkei Geka20073555035081749134714SasakiMSatoMTaguchiJA case of olfactory neuroblastoma with intracranial, intraorbital extension and multiple metastasesNo Shinkei Geka1997252163167902789415RodasRAErkman-BalisBCahillDWLate intracranial metastasis from esthesioneuroblastoma: case report and review of the literatureNeurosurgery1986194622627378560116JiangWLiuJGullanePJNon-contiguous meningeal metastases of olfactory neuroblastomaJ Neurooncol201612612012032637665317MahootiSWakelyPEJrCytopathologic features of olfactory neuroblastomaCancer2006108286921645684818SharmaSSharmaMCJohnsonMHLouMThakarASarkarCEsthesioneuroblastoma a clinicopathologic study and role of DNA topoisomerase alphaPathol Oncol Res20071321231291760737319Meis-KindblomJMStenmanGKindblomLGDifferential diagnosis of small round cell tumorsSemin Diagn Pathol1996133213241887571120MillsSEFechnerRE Undifferentiated neoplasms of the sinonasal region: Differential diagnosis based on clinical, light microscopic, immunohistochemical, and ultrastructural featuresSemin Diagn Pathol198964316328269210521BellizziAMBourneTDMillsSEStelowEBThe cytologic features of sinonasal undifferentiated carcinoma and olfactory neuroblastomaAm J Clin Pathol200812933673761828525822D cruzeLDuttaRRaoSRAVaradarajanSKuruvillaSThe role of immunohistochemistry in the analysis of the spectrum of small round cell tumours at a tertiary care centreJ Clin Diagn Res201377137713822399806923SuSYBellDHannaEYEsthesioneuroblastoma, neuroendocrine carcinoma, and sinonasal undifferentiated carcinoma: differentiation in diagnosis and treatmentInt Arch Otorhinolaryngol201418Suppl 2S149S1562599213924BellDHannaEYWeberRSNeuroendocrine neoplasms of the sinonasal regionHead Neck201638Suppl 1E2259E22662604171425MenonSPaiPSengarMAggarwalJPKaneSVSinonasal malignancies with neuroendocrine differentiation: case series and review of literatureIndian J Pathol Microbiol201053128342009021826ZhangMZhouLWangDHHuangWTWangSYDiagnosis and management of esthesioneuroblastomaORL J Otorhinolaryngol Relat Spec201072211311820453548Computed tomography scans showing a homogeneous, isolated, ill-defined soft tissue density mass in the right orbit (A C; arrows).', 'Histopathological sections of the mass.']","Figure 1 Computed tomography scans showing a homogeneous, isolated, ill-defined soft tissue density mass in the right orbit ( ; arrows). The right eyeball was displaced anteriorly and inferiorly. ( ) Axial section, ( ) sagittal section, and ( ) coronal section. AR, anterior; L, left; PI, posterior; R, right.",yes
PMC5116656,Figure_2,oa_package/20/97/PMC5116656.tar.gz,['3 cm ().'],,yes
PMC4778148,Figure_1,oa_package/40/07/PMC4778148.tar.gz,"[' 1.', '4]Periprosthetic regurgitation, n (%) None38 (95 %)9 (100 %) Trace1 (3 %)- Mild1 (3 %)- Moderate-- Severe--Prosthetic regurgitation Normal for PHV38 (95 %)9 (100 %) Trace1 (3 %)- Mild-- Moderate1 (3 %)- Severe--\nPHV prosthetic heart valve, TTE transthoracic echocardiography* Based on the Doppler continuity equation and measured left ventricular outflow tract diameter Graded according to clinical routine based on mean and peak gradients, effective orifice area, cardiac output and available PHV type and size specific reference valuesExample of biological and mechanical prosthetic heart valve imaging.', 'Transthoracic echocardiography images (a, c) and multidetector-row computed tomography images (b, d) of a normal functioning Perimount 25-mm biological valve (a, b) and a normal functioning Carbomedics 23-mm mechanical heart valve (c, d) in the aortic positionMDCTThe mean effective radiation dose was 11.', ' 1).']","Fig. 1 Example of biological and mechanical prosthetic heart valve imaging. Transthoracic echocardiography images ( , ) and multidetector-row computed tomography images ( , ) of a normal functioning Perimount 25-mm biological valve ( , ) and a normal functioning Carbomedics 23-mm mechanical heart valve ( , ) in the aortic position",yes
PMC6909519,Figure_2,oa_package/5b/0e/PMC6909519.tar.gz,"[' 2a).', ' 2b, c).', ' 2b, c).', ' 2a c).', 'EphA4 loss ameliorates social memory in APPPS1 mice.', 'Abbreviations: MWM Morris water maze, SPSN sociability/preference for social noveltyWe next studied the ability to remember social interactions, another hippocampus-dependent memory function, with the sociability/preference for social novelty (SPSN) test.', ' 2d f), although this trend did not reach statistical significance for the preference ratio in AD (p = 0.', ' 2g i).', ' 2j l).', ' 2j l).', ' 2j l).']","Fig. 2 EphA4 loss ameliorates social memory in APPPS1 mice. At 9months of age, mice were subjected to different cognitive tests to assess memory performance. Escape latency over 10 training days ( ) and time spent in the target quadrant in probe trial 1 ( ) and probe trial 2 ( ) during the MWM test (two-way RM ANOVA and two-way ANOVA with Tukeys multiple comparison test, and unpaired test to compare to chance level). Sniffing time to the empty cage versus the novel mouse and preference ratio ( ) in the sociability trial (unpaired test or Mann-Whitney test and unpaired test compared to chance level, respectively). Sniffing time to the familiar versus the novel mouse and recognition ratio ( ) in the social memory trial of the SPSN test (unpaired test or Mann-Whitney test and unpaired test compared to chance level, respectively). Total distance crossed ( ) and time spent in the small periphery ( ) and in the center ( ) of the open field exploration test (two-way ANOVA with Tukeys multiple comparison test). =2228 mice/group. In panels , , and , significant group effects (AD versus non-AD) are indicated as follows: ** 0.01, **** 0.0001. In panels and , significant effects between social subjects (novel mouse versus empty or familiar mouse) are indicated as follows: * 0.05, ** 0.01, *** 0.001, **** 0.0001. Performance above chance levels (panels , , , and ) are indicated as follows: 0.05, 0.01, 0.0001. If no asterisk or plus sign is shown in the graph, this implies no significance. Abbreviations: MWM Morris water maze, SPSN sociability/preference for social novelty",yes
PMC3569958,Figure_3,oa_package/13/89/PMC3569958.tar.gz,"['8 104 cells/cm2) grown on TCPS, chitosan membranes, and CBD-RGD modified chitosan membranes is shown in .', '35 In this study, we demonstrated that ASCs may form larger and more adhesive spheroids on RGD-modified chitosan membranes ().']","FIG. 3. The morphology of ASCs (2.810 cells/cm ) cultured on chitosan membranes (CS1 and CS2), CBD-RGDmodified chitosan membranes (CS2-RGD), or tissue culture polystyrene (TCPS). Cells were cultured on the membranes in the basal medium for 3 days and the medium was replaced by the induction medium, which contained 10M 5-azacytidine (5-aza) in the basal medium to induce cardiac differentiation. After treatment with the induction medium for 3 days, the medium was replenished by basal medium at 3-day intervals (twice). The scale bar represents 100m. CBD, cellulose-binding domain; RGD, Arg-Gly-Asp.",yes
PMC5265201,Figure_18,oa_package/a0/51/PMC5265201.tar.gz,[],Fig. 18 Bronchial obstruction due to granulomatous disease. Axial CT image of sarcoidosis demonstrating perihilar lymphadenopathy (*) and pulmonary granulomas causing extrinsic compression of several left upper lobe segmental bronchi ( ). Axial CT imaging of granulomatosis with polyangitis demonstrates infiltrative changes of the pulmonary parenchyma in a perihilar/bronchovascular distribution causing narrowing of the associated bronchi in a bilateral upper lobe predominance with sparing of the apices. The associated ground-glass opacities suggest the presence of haemorrhage,yes
PMC11426102,Figure_4,oa_package/5b/08/PMC11426102.tar.gz,"['Akt protein kinase B, CDs chronic diseases, EMT epithelial to mesenchymal transition, ER estrogen receptor , IFN- interferon- , miR microRNA, NF- B nuclear factor kappa-B, PD-L1 programmed death-ligand 1, PHLPP PH domain and leucine rich repeat protein phosphatases, PLP2 proteolipid protein 2, PPAR peroxisome proliferator-activated receptor, PTEN phosphatase and tensin homolog, ROR retinoic acid receptor related orphan receptor alpha, SIRP signal regulatory protein alpha, TGF- transforming growth factor- , TME tumor microenvironmentCancerCancer stands as a predominant global public health concern, despite notable advancements in current therapeutic modalities, the treatment of cancer remains a formidable challenge due to suboptimal therapeutic efficacy attributed to insufficient specificity and diminished bioavailability [178].']","Fig.4 Interplay between exosomes and nuclear receptors (NRs) in regulating tumor microenvironment dynamics. This figure illustrates the intricate cross-talk between exosomes and NRs in cancer pathogenesis. Tumor-derived exosomes (TDEs) convey genetic material, including genes and non-coding RNAs, to dendritic cells (DCs) and myeloid-derived suppressor cells (MDSCs). This molecular cargo targets specific NRs, influencing the functionality of these immune cells and contributing to immune evasion. TDEs from HPV HNSCC cells downregulate PPAR, activating macrophages and enhancing radiosensitivity. Chemoresistant cancer cells use exosomes to transfer miRNAs targeting NRs to wild-type cancer cells, conferring therapeutic resistance. Macrophage-derived exosomes regulate the AR/PHLPP/Akt/-catenin axis in liver cancer cells, promoting invasion. Conversely, T cell-derived exosomes deliver miR-765, suppressing PLP2 in ER uterine corpus endometrial carcinoma (UCEC) cells, reducing proliferation, EMT, and inducing apoptosis. These findings highlight the communication network between cancer cells and stromal cells within the tumor microenvironment via exosomal/NR interactions, suggesting that manipulating NR expression through targeted interventions presents a promising therapeutic strategy for diverse cancer types. Akt protein kinase B,CDs chronic diseases, EMT epithelial to mesenchymal transition, ER estrogen receptor , IFN- interferon-, miR microRNA, NF-B nuclear factor kappa-B, PD-L1 programmed death-ligand 1, PHLPP PH domain and leucine rich repeat protein phosphatases, PLP2 proteolipid protein 2, PPAR peroxisome proliferator-activated receptor, PTEN phosphatase and tensin homolog ROR retinoic acid receptor related orphan receptor alpha, SIRP signal regulatory protein alpha, TGF- transforming growth factor-, TME tumor microenvironment",yes
PMC5536904,Figure_2,oa_package/67/aa/PMC5536904.tar.gz,['.'],"Fig. 2. (A) Representative immunocytochemistry images using human-specific antibodies against 2 (P1E6) and 1 (P5D2) integrin performed on permeabilized cultured primary keratinocytes from two transgenic pigs (TG-410 and TG-458) and a non-transgenic control (WT-C3). The cells were either stained singly or in combination. DAPI was used to stain the nucleus. Detection of integrin was restricted to transgenic cells. (B) Representative images from confocal fluorescence microscopy of saponin permeabilized keratinocytes from pig TG-459 stained with the nucleus marker DAPI along with anti-human antibodies against 2 (P1E6) and 1 (P5D2) integrin. Integrin accumulates at the plasma membrane and especially at cell-cell adhesion sites, suggesting correct processing of the peptide. (C) Flow cytometry analysis of unpermeabilized keratinocytes from transgenic pigs, TG-457 and TG-459, and control pig, WT C3, stained with 2 (P1E6) or 1 (P5D2) integrin-specific antibodies. Staining solely with the secondary antibody served as a control. Representative dot plots comparing fluorescence from pig WT-C3 and TG-459 are depicted. (D) Quantitative RT-PCR on total RNA extracted from skin biopsies obtained from transgenic pigs TG-456, TG-457 and TG-459, and the non-transgenic age-matched control WT-C2. Relative mRNA levels of and are depicted normalized to endogenous . Data presented as mean valuess.d. of biological triplicates where the difference is calculated using the Student's -test.",yes
PMC4049593,Figure_1,oa_package/5d/95/PMC4049593.tar.gz,"['In comparison to FUS-WT-GFP, mutant FUS-R521C-GFP was mislocalized to the cytosol resulting in a diffuse appearance in whole mount transgenic larvae (A).', 'FUS-WT-GFP exhibited a sharply defined nuclear localization overlapping with DAPI while FUS-R521C-GFP was less confined to the nucleus and distributed throughout the cell bodies (A, bottom panels).', 'The same was observed in dispersion primary cell cultures derived from these fish: FUS-WT-GFP was confined to the nucleus of all cells in culture, while FUS-R521C-GFP was universally mislocalized to the cytosol in all cells (B).', 'In confocal images of whole zebrafish spinal cord, mislocalization of mutant FUS-R521C-GFP could also be seen in motor neurons (C, arrows).', 'g001Whole mount and cell cultures of FUS-GFP transgenic zebrafish.', 'In cells with comparable exogenous protein expression levels, FUS-WT-GFP was largely confined to cell nuclei whereas mutant FUS-R521C-GFP was 50 60% cytosolic (B; D).']",10.1371/journal.pone.0090572.g001,yes
PMC10132855,Figure_5,oa_package/54/fe/PMC10132855.tar.gz,['Three months post-surgery.'],Figure 5 Three months post-surgery.,yes
PMC9557836,Figure_5,oa_package/d1/70/PMC9557836.tar.gz,"['No differences were observed in the frequency of somatostatin, glucagon, or pancreatic polypeptide cells in either the ILD or PDG compartment from type 1 diabetes vs ND donors ().', '.']","Figure 5. No compensatory increase in hormone-expressing endocrine cells in the ILD or PDG compartments of type 1 diabetes (T1D) subjects. Frequency of glucagon+ (A), somatostatin+ (B) and pancreatic polypeptide (PP)+ cells (C) in ILD and PDG compartments. Data are presented as mean SEM for T1D (black circles, n=21) and nondiabetes (ND) donors (blue filled squares, n=21). Each data point represents an average of 1 to 3 sections per donor (from the pancreatic head, body, and/or tail regions; see Table 1). Unpaired test, >0.05 all.",yes
PMC5515959,Figure_5,oa_package/e8/34/PMC5515959.tar.gz,"[' 5b).', '\nIntegrin v 3 mediates monocyte-macrophage binding to deamidated ECM ProteinsOur observation that monocyte-macrophages expressing v 3 preferentially adhere to deamidated FN and TNC suggested that isoDGR modification of these proteins can enhance integrin binding.']",Figure 5 Microscopy images of primary human monocyte adhesion to culture plates coated with fibronectin or tenascin C native proteins (PBS-only treated controls) or the deamidated forms of these ECM components (50mM TEAB treated). Isolated monocytes were labelled with CFSE dye and the images captured using green fluorescent filter. Scale bar indicates 20m. Statistics were calculated from 4 experimental replicates.,yes
PMC4625612,Figure_5,oa_package/ad/53/PMC4625612.tar.gz,"[' 5, left sections) to no detectable eMyHC (', ' 5, right section) staining.', ' 5).', ' 5).', 'Impaired muscle regeneration in Pompe patients.', 'Two examples from classic infantile Pompe patients and DMD patients are derived from different patientsDiscussionSatellite cell numbers are neither increased nor exhausted in Pompe diseaseWe have shown that the numbers of satellite cells in skeletal muscle biopsies from Pompe patients are similar to controls.']","Fig. 5 Impaired muscle regeneration in Pompe patients. Immunofluorescent analysis of embryonic myosin heavy chain (eMyHC in red) expression in to detect actively regenerating myofibers. Muscle sections were co-stained for Laminin ( ) to visualize the fiber outline, nuclei were stained with Hoechst ( ). Representative examples are shown. Two examples from classic infantile Pompe patients and DMD patients are derived from different patients",yes
PMC4224021,Figure_9,oa_package/41/7d/PMC4224021.tar.gz,"['9; shown for CA2 9a, b, b ; 11d; iLBD vs Ctr p = 0.', '0\nQuantification of TLR2 immunoreactivity (IR) in the substantia nigra (SN) of control subjects, iLBD cases and PD patients.', '5; HC/CA2 9a-c, a -c ; 11d; iLBD vs PD p = 0.']","Figure 9 A control subject showing few TLR2 positive cells (arrow), an iLBD case showing widespread and numerous TLR2 positive cells (arrow) and a PD patient, showing again very few TLR2 positive cells (arrow), similar to controls, represent higher magnifications of ; bar =100m; =50m.",yes
PMC4025414,Figure_1,oa_package/74/d9/PMC4025414.tar.gz,"['The evaluations were performed on the most severely affected lesions and graded as none ( ), mild (+), moderate (++) or severe (+++; figure 1).', 'The stages of the pathological changes correspond to those in figure 1.']","Figure1 Measures of degeneration in the upper motor neuron system. (AD) Loss of Betz cells in the primary motor cortex: stage (), the Betz cells were spared in number and gliosis was absent (A); stage (+), mild neuronophagia and gliosis were noted (B) and stage (++), marked neuronophagia and glial proliferation were observed (C). (DK) Aggregation of CD68 macrophages in the primary motor cortex (DG) and the corticospinal tract in the lateral column of the spinal cord (HK): stage (), the aggregates were absent (D and H); stage (+), the aggregates were occasionally present (E and I); stage (++), the aggregates were present at a number of 15/ 100 field (F and J) and stage (+++), the aggregates were diffusely observed (G and K). (LO) Myelin pallor in the corticospinal tracts (CST) of the lateral column of the spinal cord: stage (), myelin pallor was not detected (L); stage (+), myelin pallor was slightly notable (M); stage (++), myelin pallor was moderate (N) and stage (+++), the CST was entirely pale. (AC) H&E staining, (DK) anti-CD68 immunohistochemistry and (LO) Klver-Barrera staining. Scale bars: (AG) 100m, (HK) 50m and (LO) 3mm.",yes
PMC3843600,Figure_1,oa_package/3f/d6/PMC3843600.tar.gz,"['He underwent a coronary arteriogram a week later, which revealed a fistula connection arising from the LAD and draining into the main pulmonary artery, blockage of the right coronary artery and significant stenoses on the LAD and LCX (A, 1B).', 'KoneruJSamuelAJoshiMCoronary Anomaly and Coronary Artery fistula as cause of Angina Pectoris with Literature ReviewCase Rep Vasc Med2011201148618722937462.']","Figure 1. ( ) Coronary angiography confirms that fistula between left anterior descending coronary artery (LAD) and main Pulmonary Artery (PA), also, stenoses on the LAD and left circumflex artery (LCX). ( ) Fistula which located between left anterior descending coronary artery(LAD) and main pulmonary artery (PA).",yes
PMC3487992,Figure_1,oa_package/59/82/PMC3487992.tar.gz,['Key features of placental malaria.'],Figure 1 Haematoxylin and eosin stained placental sections from the Mbarara trial are shown at 600X. Normal placental histology. Parasitized erythrocytes sequester in the intervillous space. Monocyte-macrophages accumulate and phagocytose parasitized erythrocytes and haemozoin. Monocyte macrophages become enmeshed in fibrin and degenerate. Residual haemozoin persists in fibrin following successful treatment.,yes
PMC8397170,Figure_2,oa_package/90/f5/PMC8397170.tar.gz,"['Type IV: trifurcation of the main trunk with supplying branches to both sides of the anal canal in some with a minor participation of MRA to the vascularization of the CCR ().', 'Forty-three-year-old lady, weight lifter, suffering from grade III bleeding hemorrhoids: (A) Angiogram of SRA showing a type IV vascularization according to the Thomson classification After the coiling of left lateral branch of SRA (arrow), a selective angiogram of left internal iliac artery showing a patent left MRA.']","Figure 2 Forty-three-year-old lady, weight lifter, suffering from grade III bleeding hemorrhoids: ( ) Angiogram of SRA showing a type IV vascularization according to the Thomson classification After the coiling of left lateral branch of SRA (arrow), a selective angiogram of left internal iliac artery showing a patent left MRA. ( ) Coiling of right lateral branch of SRA (arrow). ( ) of the CCR feeding artery below the pubic symphysis. ( ) The final angiogram of SRA showing coiling of right lateral branch of SRA (arrow), coiling of left MRA (asterisk), and coiling of left lateral branch of SRA (arrowhead), with occlusion with evidence of retrograde filling of right MRA (arrow) and left MRA (arrowhead). Note the diameter of left MRA is equal to that of the SRA.",yes
PMC4389548,Figure_3,oa_package/b5/b2/PMC4389548.tar.gz,"['Stx2 interacts with murine glomerular renal cells in vitro.', 'Examples of numbers and distributions of yellow clusters are shown in C.']","Figure 3 Murine podocytes or Vero cells were incubated with 20 nM Stx2 for 0 (no toxin), 5 and 15 min at 37C. Stx2 was labeled with monoclonal antibody 11E10 followed by anti-mouse IgG-AlexaFluor 647 (pseudo colored red) and Gb was labeled with monoclonal antibody 38.13 followed by anti-rat IgM-AlexaFluor 488 (green). Nuclei were stained with DAPI (blue). Samples were observed with confocal microscopy. Representative cells that are chosen from each time point group are shown. Bars indicate 10 m. Arrowheads point Stx2-AlexaFluor 647 positive cells. Murine podocytes were incubated with 20 nM Stx2 for 0 (no toxin), 5 and 15 min at 37C and labeled as above. Samples were visualized with 3D STORM-TIRF microscopy and analyzed using Nikon Elements software. Whole cell view presents representative cells from each time point. Bars are 10 m. Yellow spot view presents highly magnified and three dimensionally shown yellow cluster (red = Stx2-Alexa647, green = Gb -Alexa488 and yellow = red and green overlapping pixels). Bars are 100 pixels (px). Yellow cluster depth view depicts the intra or extracellular depth of the yellow cluster. The color-coded depth scale at the left differentiate the distance (nm) from the focus plane (0 nm, green). Blue represents intracellular (up to 500 nm) whereas red represents extracellular (up to -500 nm). Contact count given in the graphs presents amount and depth distribution of green and red contact of a whole cell. The color-coded depth scale at the bottom indicates intracellular (blue) to extracellular (red).",yes
PMC3649965,Figure_2,oa_package/db/70/PMC3649965.tar.gz,"['Macroscopic examination of lungs showed multifocal pulmonary consolidation and hemorrhage affecting at least one lobe in all animals euthanized 9 or 13 dpi (A).', 'Diffuse EGFP fluorescence was detected in an SVV-EGFP infected monkey at 9 dpi (B and C).', 'Combined immunohistochemical (IHC) and immunofluorescence (IF) analyses for SVV antigens and EGFP on consecutive sections of lung showed that EGFP expression was restricted to SVV antigen-positive cells (D G), demonstrating that EGFP is a valid marker to identify SVV-infected cells in the monkeys.', 'At 9 dpi, abundant SVVposkeratinpos lung epithelial cells were observed (H), as well as SVVposCD3pos T-cells (', 'In addition, SVV antigens were found in intra-alveolar cells that co-expressed CD68 and/or CD11c, consistent with alveolar macrophages (AM), some of which appeared to have phagocytosed SVV-infected cells (J).', 'Occasionally, SVVposCD11cpos dendritic cell (DC)-like cells displaying multiple branched projections were observed adjacent to bronchi (K).', 'g002Macroscopic and microscopic detection of SVV-infected cells in lungs of infected African green monkeys.']",10.1371/journal.ppat.1003368.g002,yes
PMC7251917,Figure_4,oa_package/25/ca/PMC7251917.tar.gz,"[' 4a).', '4b).', '4b).', 'Relative expression of cardiomyocyte-specific genes and ion channel genes by qRT-PCR.', '001DiscussionElectrical signals are known to play an important role during cardiac development in embryonic tissue [21, 22].', '4).']","Fig. 4 Relative expression of cardiomyocyte-specific genes and ion channel genes by qRT-PCR. The expression levels of cardiomyocyte-specific genes in the electrical stimulation group were higher than those in the control group. The expression levels of ion channel genes except KCNJ2 and KCNJ12 in the electrical stimulation group were significantly increased at day 14 compared to those in the control. *, <0.05; **, <0.01; ***, <0.001",yes
PMC6966561,Figure_3,oa_package/0a/26/PMC6966561.tar.gz,"['0457) in the anterior and posterior tubules, respectively (Table 1, A,B).', '0017) in the anterior and posterior tubules, respectively (Table 1, A,B).', '0014, Table 1, A,B).', '1877, Table 1, A,B).', 'Smac-mimics reduced cysts in BicC /YC33 flies.']","Figure 2 Testing protocol for Smac-mimic efficacy in cyst reduction. wild-type, , and flies (02 days old) were placed in food-containing vials at age 02 days and transferred into fresh vials every three days. Once the age of 79 days was reached, flies were placed in vials containing one of each of the Smac-mimics or vehicle control, respectively. Cysts were scored on the micro-dissected MTs after 20 days, when flies reached age 2729 days.",yes
PMC2173241,Figure_5,oa_package/b3/b4/PMC2173241.tar.gz,"['Total-cell (left) and cross sectional (right) confocal images of the sodium channel subunit staining pattern are shown for two examples of uninfected DRG neurons ( a), and for two examples of neurons infected with HSV-1 for 24 h (', 'The mean density of fluorescence was quantified for HSV-1 infected and uninfected neurons from the total-cell images as shown in c.', '.']","Figure 5. (a) The left panel is the collapsed image of several serial scans showing the total neuron distribution of sodium channel subunits of uninfected neurons, and the right panel is a single z-axis image showing the cross section of an uninfected neuron demonstrating plasma membrane staining. (b) The left panel is the collapsed image of several serial scans showing the total neuron distribution of sodium channel subunits of an HSV-1infected neuron, and the right panel is a single z-axis image showing the cross section of the infected neurons. (c) The mean fluorescence intensity of stained HSV-1infected and uninfected neurons. The mean intensity of fluorescence was significantly reduced in infected neurons ( test, P < 0.00001).",yes
PMC6251248,Figure_16,oa_package/8e/1d/PMC6251248.tar.gz,[],"Figure 16 Sarcoid sialadenitis. A 51-year-old male with a history of pulmonary sarcoidosis presents with bilateral parotid swelling. Coronal T1 FS + C images demonstrate marked enlargement and hyperenhancement of the bilateral parotid glands (white arrows), presumably reflecting sarcoid sialadenitis.",yes
PMC6165196,Figure_4,oa_package/c3/c8/PMC6165196.tar.gz,"['The levels of Mfsd2a mRNA and protein in the brain were not significantly different between Wt and 5xFAD (A,B) and no changes were observed following the FO treatment.', 'In the liver (C,D), strong trends of enhanced translation of Mfsd2a mRNA to protein were observed after FO supplementation, but these changes were not significant due to liver individual differences.', 'Expression of Mfsd2a in the brain and liver of Wt and 5xFAD mice after fish oil supplementation.']","Figure 4 Expression of Mfsd2a in the brain and liver of Wt and 5xFAD mice after fish oil supplementation. The levels of Mfsd2a mRNA and protein were obtained by real-time polymerase chain reaction (RT-PCR) ( , ) and Western blotting ( , ), respectively in the brain (left panel) and liver (right panel) of Wt and 5xFAD animals. 03B2-actin served as an internal control of the mfsd2a gene expression and protein load. Data are presented as mean SEM (standard error of mean). * < 0.05; n.s., not significant.",yes
PMC1906797,Figure_3,oa_package/79/c3/PMC1906797.tar.gz,['Mechanisms involved in the regulation of CD36 expression by tumor necrosis factor (TNF) .'],"Figure 3 Mechanisms involved in the regulation of CD36 expression by tumor necrosis factor (TNF). TNF inhibits both basal and rosiglitazone-induced peroxisome proliferator-activated receptor (PPAR) activation. Human monocytes were incubated with macrophage-serum-free medium (M-SFM) alone (control), or with M-SFM containing TNF (10 ng/ml) for 5, 30 or 60 minutes, and then stimulated, or not, with a synthetic ligand of PPAR, rosiglitazone (R; 5 mol/l) for 45 minutes. Nuclear proteins were isolated and a [- P]ATP labeled oligonucleotide expressing the PPAR DNA consensus binding sequence was added. PPAR activation was analyzed by gel-shift assay. TNF inhibits both basal and rosiglitazone-induced CD36 mRNA expression. Human monocytes were incubated with M-SFM alone (control), or with M-SFM containing TNF (10 ng/ml) for 1 h and then stimulated or not with rosiglitazone (R; 5 mol/l) for 4 h. CD36 mRNA expression was quantified using RT-PCR and normalized to -actin. Data represent the mean standard error (SE) of the relative quantification of CD36 mRNA expression measured in three experiments. *Significantly different from control ( < 0.05). TNF induces reactive oxygen species production. Monocytes were incubated with Hanks balanced salt solution (HBSS) alone (control), or with HBSS containing TNF (10 ng/ml) for 1 h. Reactive oxygen species production was measured by chemiluminescence in the presence of 5-amino-2,3-dihydro-1,4-phthalazinedione in a thermostatically controlled luminometer. Data represent total chemiluminescence emission (area under the curve) for 1 h, measured in three experiments. *Significantly different from the control ( < 0.05). The decrease in CD36 membrane expression induced by TNF is not inhibited by an anti-oxidant (Trolox). Monocytes were incubated with M-SFM alone (control), or with M-SFM containing either TNF (10 ng/ml), Trolox (1 M), or TNF combined with Trolox for 24 h and the membrane expression of CD36 expression was quantified using flow cytometry. Data represent the geometric mean SE of the fluorescence measured in three experiments in duplicate. *Significantly different from the control ( < 0.05).",yes
PMC9649780,Figure_1,oa_package/29/22/PMC9649780.tar.gz,"[' 1a e), while extensive sonication is needed to yield larger populations of L2 and L3 fibrils (', 'Cryo-EM structures of the L1 Syn fibrils.', 'Cryo-EM structures of L2 and L3 Syn fibrils.', ' 1a for details.', ' 1b d, Supplementary ', ' 1e).', ' 1f h), corresponding to surface-bound phospholipids (for details, see below).', ' 1b), L1B and L1C fibrils are composed of two identical and intertwined L1 protofilaments (', ' 1c, d).', ' 1f h, 2g i, 3a), which together are reminiscent of the cross-section of phospholipid micelles.']","Fig. 1 Cryo-EM structures of the L1 Syn fibrils. Sequence and secondary structure of human Syn. Familial PD mutation sites (black arrow) localized within the lipid binding N-terminal region (residues 160) . Green-colored residues bind to the lipid acyl chain, blue to the choline moiety , and gray were not resolved. Cryo-EM structures of L1A ( ), L1B ( ), and L1C ( ) fibrils (protofilaments colored differently). The atomic models are shown as sticks. Labels denote the fibril width, the helical twist and rise, and residue numbers. The density maps in the lower panels are displayed using the carve feature in PyMOL at a distance of 2. Backbone of the L1 fibrils with the 110 colored magenta and loops in gray. Overlay of a sharpened high-resolution map shown in magenta ( ), purple ( ), and brown ( ) and an unsharpened, 4.5 low-pass filtered density in gray. The backbone is shown as a black ribbon. Densities highlighted with a yellow background are reminiscent of lipid micelles.",yes
PMC11413010,Figure_7,oa_package/00/64/PMC11413010.tar.gz,"['7a.', 'cHA- NS1-LAIV vaccination activates tissue-resident memory (TRM) IAV-NP+CD8+ T-cells on day 3 post-infection.', 'The data indicated the rapid expansion of IAV-specific CD8+ TRM (CD3 + CD8+ CD44+ CD69+ CD103+ IAV-NP+) in group 1 that received cH8/1- NS1 prime followed by cH11/1- NS1 boost, suggesting efficient priming by our intranasal vaccine regimen.', '7b).']","Fig. 7 cHA-NS1-LAIV vaccination activates tissue-resident memory (TRM) IAV-NP CD8 T-cells on day 3 post-infection. Four weeks post-final-boost, mice were challenged with a QIV matched IVR-180 using a lethal dose of 100 LD . A representative image of the gating strategy used to probe lung resident NP-tetramer+ CD8 T-cell responses of animals on day 3 post-infection. The following markers were utilized for the gating strategy; CD3eT cell marker, CD8aCD8 T cell marker, CD69Activation marker, CD44Memory marker, CD103Tissue resident marker (absent in CD4+) Absolute count of TRM IAV-NP CD8 T-cells 3 days post infection. Each dot is one animal and data are shown as meanSEM Statistical significance was compared to QIV standard of care group (Group 7) using one-way ANOVA using Dunnetts correction. **** - <0.0001.",yes
PMC3613076,Figure_1,oa_package/85/5d/PMC3613076.tar.gz,"['05 versus the normal control animals, see ).', '0-34249805700Treatment with the ELR-CXC chemokine antagonist G31P reduces pulmonary neutrophil responses in guinea pigs with Klebsiella pneumoniae.']","Figure 1 Treatment with the ELR-CXC chemokine antagonist G31P reduces pulmonary neutrophil responses in guinea pigs with . Guinea pigs were challenged with and treated with G31P. The airway neutrophil responses were assessed from differential counts of cells recovered by bronchoalveolar lavage (BAL). The airway (BAL fluid) levels of myeloperoxidase (MPO), as surrogate measurements of neutrophilic inflammation, were assessed using a chromogenic assay. The G31P treatments reduced each of these indicators of neutrophilic inflammation close to normal. This experiment was repeated three times; the data depicted are representative of each group.",yes
PMC4919923,Figure_1,oa_package/35/5b/PMC4919923.tar.gz,"['The assignment of the 1H, 15N HSQC spectrum has been a long-time technical effort that we started 10 years ago [23,24,25,26,27], and we have been joined in this effort by other research groups [28,29,30] to such a point that the spectrum of the longest isoform (Tau441, A) has been completely assigned.', 'NMR Spectroscopy of Isolated TauThe 1H, 15N HSQC spectrum of Tau is characterized by a very narrow range of chemical shift values for the amide protons ().', 'As an example, we show in B a superposition of the spectra of wt Tau441 and its P301L mutant, whereby the latter leads to an early form of dementia called FTDP-17 [47,48].', 'In addition, the resonance of phospho-Thr231 splits up in five or more peaks when Tau is phosphorylated on other positions by an activated CDK or Erk kinase (C).', 'Connecting two of these short binding fragments led to the TauF4 (Tau 208 324) construct (A) which not only binds more tightly than full-length Tau to the MT surface but equally is very efficient in MT assembly [80].', '0021521\n21731772(A) Schematic view of the primary sequence of Tau441, the longest isoform of Tau.']","Figure 1 ( ) Schematic view of the primary sequence of Tau441, the longest isoform of Tau. Different isoforms are characterized by the insertion of 0, one or two N-terminal inserts (N1 and N2), and three (R1-R3-R4) or four (R1-4) repeats (leading to the 3R or 4R forms). The repeats are preceded by a proline-rich region (PRR). The two hexapeptides PHF6 and PHF6* are indicated as red rectangles in R1 and R2, whereas Taus two cysteine residues (Cys291 and Cys322) are indicated as yellow circles in R2 and R3. The fragment TauF4 spans part of the PRR, the first two repeats and a small part of R3; ( ) The H, N HSQC spectra of N-labeled wild-type (black) and N, C-labeled P301L (red) Tau441 show that only a couple of residues directly adjacent to the mutation show chemical shift differences. Residues Val -Tyr -Lys of the PHF6 peptide are identical in chemical shift and intensity in both proteins; ( ) Zoom of the H, N spectrum of CDK2 phosphorylated Tau around the resonance of pThr231, showing several peaks for the same phosphorylated residue.",yes
PMC10026180,Figure_8,oa_package/9d/3a/PMC10026180.tar.gz,"['(A) Erythematous papulonodular eruption on the buttocks, lower back, and hip with scale and hemorrhagic crusts in a 10-month-old boy with X-linked CGD, consistent with molluscum contagiosum.']","Figure 8 ( ) Erythematous papulonodular eruption on the buttocks, lower back, and hip with scale and hemorrhagic crusts in a 10-month-old boy with X-linked CGD, consistent with molluscum contagiosum. (B) Post-treatment with IV cidofovir and a resolution of the lesions with the remaining postinflammatory changes and mild scarring. CGD, chronic granulomatous disease.",yes
PMC9320483,Figure_9,oa_package/72/e8/PMC9320483.tar.gz,"['In , a selection of tiles is shown with their corresponding model BD output.', 'Output examples for some of the tiles ( 1024 m 1024 m) from .']","Figure 9 Output examples for some of the tiles ( 1024 1024 ) from . From left to right: original tile, the output of the network (only BD), post-processing, the binary result after applying a threshold of .",yes
PMC8404122,Figure_16,oa_package/e1/32/PMC8404122.tar.gz,[],Figure-16 Paralysis of the urinary bladder in a lamb: (a) Incontinence with dribbling of urine as the main complaint; (b) cloudy urine sample; (c) urine sediment formed after centrifugation.,yes
PMC8295969,Figure_1,oa_package/79/04/PMC8295969.tar.gz,"['Blood flow signals appeared as more intense and star- or dot-shaped centrally and peripherally; therefore, a malignant nodule was suspected (a).', '4-mm2, oval-shaped, anechoic, cystic lesion with well-defined margins was noted at the inferior aspect of the thyroid that was superior to the suspicious malignant nodule (b).', '\n6\n.', '1177_03000605211031430-fig1"" position=""float""/>Pathological resultsThe frozen specimen revealed multiple cysts with interstitial fibroses and vitreous degeneration.']",Figure 1. (a) Ultrasonography in the longitudinal plane showing a 12-mm nodule at the uppermost part of the left thyroid gland (arrows). (b) An oval nodule inferior to the thyroid gland measuring 5.43.4 mm with well-defined boundaries and homogeneous echo (arrows).,yes
PMC11431781,Figure_3,oa_package/ba/34/PMC11431781.tar.gz,"['Addition of 4-OH-tamoxifen efficiently reduced the expression of ZC4H2 in MSCs (A).', 'The results showed that loss of ZC4H2 had no significant effect on the osteogenic differentiation of MSCs (B E).', 'In the histochemical assays, the staining of ALP and ARS may seem more reduced in the KO group than in the control cells (B,D).', 'Indeed, in the quantified assays normalized to total protein levels (C,E), no clear difference was observed between these two groups.', 'No significant change in their expression was observed in the ZC4H2 KO group (F).', 'The effect of ZC4H2 knockdown on osteogenic differentiation of MSCs.']","Figure 3 The effect of knockdown on osteogenic differentiation of MSCs. ( ) 4-OH-tamoxifen (4-OHT) treatment efficiently induced loss of expression in the MSCs ( ; Rosa26-CreERT2) as detected by qRT-PCR at 24-h. ( , ) ALP staining and quantitative analysis of osteogenic differentiation in control and KO MSCs. ( , ) ARS staining and quantitative analysis of osteogenic differentiation in control and KO MSCs. ( ) The expression levels of genes related to osteogenic differentiation in differentiated control and KO MSCs. Data represent mean SD, *** < 0.001. Scale bars, 2 mm in ( , ).",yes
PMC3098885,Figure_6,oa_package/49/5f/PMC3098885.tar.gz,"['Therefore, we performed immunohistochemical analyses using brain sections of controls and mutants characterized by the presence (aspa / ) or absence (aspalacZ/lacZ) of ASPA immunoreactivity ().', 'Microglia activation in the mutant thalamus was detected by Iba-1 immunohistochemistry ().', 'g006Reactive gliosis in aspalacZ/lacZ mice.', 'This decrease was not due to a reduction in astrocyte numbers () and might represent a cellular mechanism to prevent additional generation of NAA from NAAG in an environment of pathologically enriched NAA.']",10.1371/journal.pone.0020336.g006,yes
PMC7658141,Figure_1,oa_package/94/23/PMC7658141.tar.gz,"['05) (Table 1, ).', '89)BAP1, Ki-67 index and Id-1 protein expression under immunohistochemical staining ( 200).']","Figure 1 BAP1, Ki-67 index and Id-1 protein expression under immunohistochemical staining (200). (A,B) Respectively BAP1 protein expression in carcinoma adjacent tissues and CCRCC organizations; (C,D) expression of KI-67 index in adjacent tissues and CCRCC cancer tissues, respectively; (E,F) expression of Id-1 protein in adjacent tissue and CCRCC cancer tissue, respectively.",yes
PMC2527007,Figure_1,oa_package/3f/02/PMC2527007.tar.gz,['CATS- and CATX-immunohistochemistry in normal rat spinal cord.'],"Figure 1 . Representative examples of CATS- (A, C, E-G) and CATX-immunostained (B, D, H-J) sections of the L5 segment. CATS-immunopositive deposits are localized in small glial-like cells (C, E, F) that distributed homogenously throughout the section (A), while CATX is mostly found in large neurons (D, H) and only few small cells are intensely stained (D, J). G, I: Sections incubated with preabsorbed primary antibodies are free of immunostaining. Scale bars, 500 m (A, B), 50 m (C, D), 20 m (E-J).",yes
PMC10721297,Figure_2,oa_package/9a/f5/PMC10721297.tar.gz,[' CT of the same patient with a subdural hemorrhage.'],Figure 2 CT of the same patient with a subdural hemorrhage.,yes
PMC11044050,Figure_4,oa_package/49/56/PMC11044050.tar.gz,"['Additionally, illustrates the corresponding visualizations of one of the top features of MR (original_collage2D_glcmV_JointEnergyEntorpy) and MP (Shape: 5 %/95 % invariant 1).', 'The presence of more chaotic intensity gradient orientations quantified by CoLlAGe radiomic feature on ADC (: R3, R4) suggests more aggressive PCa with a higher risk of rising PSA as compared to the ones with more uniform and lower entropy regions (', 'Similarly, the pathomic visualizations of Shape: 5 %/95 % invariant 1 (a gland shape feature) illustrates that high-risk rising PSA patients with aggressive cancer may present uniformly small, malformed lumen, resulting in lower 5th/95th percentile ratios (lower range) (: P3, P4) as compared to cases with lower risk of rising PSA (', 'Visualizations of one of the topmost features of MR (orginal_collage2D_glcmV_JointEnergyEntorpy) (R1-R4) and MP (Shape: 5 %/95 % invariant 1) (P1 P4) between four different patients.', 'It can be observed that the visualizations of Co-occurrence of Local Anisotropic Gradient Orientations (CoLlAGe) gray level cooccurrence matrix (GLCM) radiomic feature on apparent diffusion coefficient (ADC) maps indicates the presence of higher density of high entropy regions for which MR has classified as rPSA+ (: R3, R4), as compared to the ones for which MR has classified as rPSA (', 'Similarly, the pathomic visualizations of Shape: 5 %/95 % invariant 1 depicts that a high risk of rising PSA with more aggressive cancer leads to uniformly small, malformed lumen resulting in a lower 5th/95th percentile ratio (lower range) (: P3, P4) as compared to cases with lower risk of rising PSA (', 'Additionally, when top radiomic features from MR (including only T2W MRI features: a GLCM and two Gabor-based based features) and MP were used to train classifiers ERaP (trained using combination of top radiomic and pathomic features on D1), ER (trained with top radiomics features extracted solely from D1) and EP (trained with top pathomic features solely from D1), ERaP (AUC = 0.', 'Pathomic feature visualizations of one of the top pathomic features, Shape: 5 %/95 % invariant 1 () depicts that high risk of rising PSA with more aggressive cancer leads to uniformly small, malformed lumen resulting in lower 5th/95th percentile ratio (lower range) (', '4: P3, P4) as compared to cases with lower risk of rising PSA (: P1, P2).']","Fig. 4 Visualizations of one of the topmost features of M (orginal_collage2D_glcmV_JointEnergyEntorpy) (R1-R4) and M (Shape: 5%/95% invariant 1) (P1P4) between four different patients. The columns 1,2 represent patients with a low risk of rising PSA and columns 3,4 represent patients with a high risk of rising PSA. It can be observed that the visualizations of Co-occurrence of Local Anisotropic Gradient Orientations (CoLlAGe) gray level cooccurrence matrix (GLCM) radiomic feature on apparent diffusion coefficient (ADC) maps indicates the presence of higher density of high entropy regions for which M has classified as rPSA ( : R3, R4), as compared to the ones for which M has classified as rPSA ( : R1, R2). Similarly, the pathomic visualizations of Shape: 5%/95% invariant 1 depicts that a high risk of rising PSA with more aggressive cancer leads to uniformly small, malformed lumen resulting in a lower 5th/95th percentile ratio (lower range) ( : P3, P4) as compared to cases with lower risk of rising PSA ( : P1, P2). For radiomic visualizations, the feature array output from the pyradiomics package was used to overlay on top of the ADC using matplotlib package. For pathomics visualizations, in-house MATLAB code was used to overlay the visualizations.",yes
PMC3350025,Figure_4,oa_package/a7/4a/PMC3350025.tar.gz,"['A subsequent PET/CT scan, nine months after completion of radiation therapy, showed increasing metabolic activity at the previous site of disease ().', '003""/>Posttreatment planar PET/CT image demonstrating recurrence of PET-avidity at site of original disease.']",Figure 4 Posttreatment planar PET/CT image demonstrating recurrence of PET-avidity at site of original disease.,yes
PMC3622059,Figure_2,oa_package/da/6e/PMC3622059.tar.gz,['Ratiometric recording of the Cl-Sensor fluorescence using a non-adjusted fluorescence setup.'],"Figure 2 Scheme of the protocol of experiment. Cl-Sensor was excited every 20 s using two consecutive light pulses through first 430 nm and then 500 nm excitation filters. Examples of fluorescence images obtained by excitation at 430 nm and 500 nm at different exposure times as indicated. Plots illustrate the mean SEM of fluorescence intensity measured on the transfected cells (signal) and region free of cells (background) ( = 15 regions measured in the same sample). Fluorescence responses of individual cells to 5 min application of the external solution containing 50 M glycine and 100 mM KCl. Examples were chosen to illustrate the variability of fluorescence intensity and signal ratio. Ratio values (R ) were obtained by arithmetic division of fluorescence induced by Cl-Sensor excitation via 430 and 500 nm filters. Left and middle plots show normalized mean SEM fluorescence responses from 36 cells recorded in the optical field during excitation with 430 nm (left) and 500 nm (middle) filters, respectively. Values were obtained as difference of each individual point with first recorded value of the experiment ( F) divided per first recorded value (F). The right plot illustrates mean SEM of signal ratio R . The first points on the plots correspond to the first image taken in the experiment.",yes
PMC7483032,Figure_5,oa_package/cb/5b/PMC7483032.tar.gz,"['Cytologic smears of SPN are usually discohesive and hypercellular, and the cells demonstrate mild nuclear atypia and plasma cell-like features (A).', 'However, it is relatively uncommon to see the typical myxoid pseudopapillary pattern of SPN in cytologic smears of EUS-FNA (B).', '.']",Fig. 5. (A) Scattered solid pseudopapillary neoplasm cells show discohesive organoid pattern with vesicular nuclei. (B) Solid pseudopapillary neoplasms show myxoid pseudopapillae with scattered plasmacytoid cells.,yes
PMC9648651,Figure_9,oa_package/db/db/PMC9648651.tar.gz,"['The second author who published about this procedure was Jacques Van der Meulen, 1979,[14] he named it medial faciotomy [b].']",Figure 9 Facial bipartition history: (A) Obwegesers unique Antonio case and (B) Van der Meulens medial faciotomy,yes
PMC10519440,Figure_3,oa_package/fc/58/PMC10519440.tar.gz,[' Upper lobe lung nodules Ventriculomegaly and diffuse parenchymal volume lossDilatation of the lateral ventricles (green arrows) and sulcal prominence (red arrows) on non-contrast CT of the head.'],Figure 3 Upper lobe lung nodules,yes
PMC3169809,Figure_3,oa_package/ba/ee/PMC3169809.tar.gz,"['002""/>Occlusal view of the mandibular arch (pretreatment mirror view).']",Figure 3 Occlusal view of the mandibular arch (pretreatment mirror view).,yes
PMC7732290,Figure_5,oa_package/92/2c/PMC7732290.tar.gz,"['2) (A 5C).', '1) (D 5F).', '4) for CD8+ cells (G 5I).', 'Comparison of CD3+, CD4+ and CD8+ cells with physiological aging in entorhinal cortex.']","Figure 5 Representative images and quantification of CD3 ( ), CD4 ( ) and CD8 ( ) expression in young and aged individuals. Data are presented as number of positive cells per mm with median bar. Red scale bar: 50 m; black scale bar: 100 m. Entorhinal cortex (EC), perivascular region (pv), brain parenchyma (pa).",yes
PMC5321265,Figure_4,oa_package/55/b2/PMC5321265.tar.gz,"['01; A) and an interaction genotype day in the loss of balance (F6,210 = 2.', '03; B), backward locomotion (F6,210 = 5.', '0001; C) and grooming (F6,210 = 2.', '01, D), while no differences were found in the other parameters analyzed (', 'Interestingly, post-hoc tests revealed a higher frequency of abnormal events in KO mice when compared to HET and WT starting from P8, a crucial window for motor development, since at this time point animals start moving all four paws on the floor (see circling and locomotion in E).', 'The most critical picture was seen at P16, when a mature motor profile was reached by all three genotypes with locomotion and wall climbing behaviors (see E).', 'Given the motor abnormalities observed during spontaneous behavior, we investigated the righting reflex that requires a complex coordination between head, trunk, and paws (F).', 'No genotype differences were found in the righting reflex latency, suggesting a normal motor coordination development in PRRT2 mouse line (F).', '03; G) and backward locomotion (genotype effect F2,32 = 4.', '01; H; Movie S1), with no differences in grooming and locomotion (', '']","Fig. 4 Motor behavioral patterns shown by PRRT2 mouse line during postnatal development and at adulthood. AD. A higher frequency of bouncing (A), loss of balance (B), back walking (C) and a longer duration of grooming (D) were observed in KO mouse pups as compared with HET and WT littermates during 3-min maternal separation at P4, P8, P12 and P16. E. The panel represents the percentage of time spent by WT, HET and KO pups performing the various behaviors analyzed at P4, P8, P12 and P16. F. Righting reflex latencies measured at the end of the maternal separation test. N=14 WT, 43 HET and 17 KO mouse pups. GH. A higher frequency of loss of balance (G) and back walking (H) was still present in adult PRRT2 KO mice. N=8 WT, 14 HET and 13 KO male mice. All data are expressed as meansSEM; *p<0.05, **p<0.01, ***p<0.001; two-way ANOVA and post-hoc Fisher PLSD test.",yes
PMC11431435,Figure_10,oa_package/b2/5c/PMC11431435.tar.gz,[],"Figure 10 Summary of the main findings and suggested pathogenesis of long-standing groin pain. ( ) Histological analysis of pubic plates in this study suggested that the cartilage component of the pubic plate extends from the pubic symphysis. ( ) Repeated traction and shearing forces due to kicking and cutting movements with pelvic twisting cause damage to the cartilage in the pubic symphysis to the pubic plate, resulting in long-standing groin pain. PS: pubic symphysis, PP: pubic plate.",yes
PMC6078075,Figure_1,oa_package/08/36/PMC6078075.tar.gz,"['Despite their limitations, previous skin-permeation investigations have revealed the main skin-delivery routes of transdermal NPs, which are shown in .', 'Studies have demonstrated that penetration may be enhanced in damaged skin ().', 'In addition, the smaller the metallic NPs were, the stronger the immunoreaction was ().', 'J Control Release20162423152744974342KuboANagaoKAmagaiMEpidermal barrier dysfunction and cutaneous sensitization in atopic diseasesJ Clin Invest201212224404472229318243XieGLuWLuDPenetration of titanium dioxide nanoparticles through slightly damaged skin in vitro and in vivoJ Appl Biomater Funct Mater2015134e356e3612661675344Miquel-JeanjeanCCr pelFRaufastVPenetration study of formulated nanosized titanium dioxide in models of damaged and sun-irradiated skinsPhotochem Photobiol2012886151315212264239545IlvesMPalom kiJVippolaMTopically applied ZnO nanoparticles suppress allergen induced skin inflammation but induce vigorous IgE production in the atopic dermatitis mouse modelPart Fibre Toxicol201411382512323546VogtACombadiereBHadamS40 nm, but not 750 or 1,500 nm, nanoparticles enter epidermal CD1a+ cells after transcutaneous application on human skinJ Invest Dermatol20061266131613221661472747KunzmannAAnderssonBThurnherrTKrugHScheyniusAFadeelBToxicology of engineered nanomaterials: focus on biocompatibility, biodistribution and biodegradationBiochim Biophys Acta18102011336137348ThyssenJPMenn TMetal allergy: a review on exposures, penetration, genetics, prevalence, and clinical implicationsChem Res Toxicol20102323093181983142249FilonFLBelloDCherrieJWSleeuwenhoekASpaanSBrouwerDHOccupational dermal exposure to nanoparticles and nano-enabled products part I: factors affecting skin absorptionInt J Hyg Environ Health201621965365442728958150JourneayWSGoldmanRHOccupational handling of nickel nanoparticles: a case reportAm J Ind Med2014579107310762480959451SmuldersSGolanskiLSmoldersEVanoirbeekJHoetPHNano-TiO2 modulates the dermal sensitization potency of dinitrochlorobenzene after topical exposureBr J Dermatol201517223923992506006352AuttachoatWMcLoughlinCEWhiteKLSmithMJRoute-dependent systemic and local immune effects following exposure to solutions prepared from titanium dioxide nanoparticlesJ Immunotoxicol20141132732822413449253KarlbergATB rjeAJohansenJDActivation of non-sensitizing or low-sensitizing fragrance substances into potent sensitizers-prehaptens and prohaptensContact Dermatitis20136963233342410714754FageSWMurisJJakobsenSSThyssenJPTitanium: a review on exposure, release, penetration, allergy, epidemiology, and clinical reactivityContact Dermatitis20167463233452702739855JamesSAFeltisBNde JongeMDQuantification of ZnO nanoparticle uptake, distribution, and dissolution within individual human macrophagesACS Nano201371210621106352418795956WinterMBeerHDHornungVKr merUSchinsRPF rsterIActivation of the inflammasome by amorphous silica and TiO2 nanoparticles in murine dendritic cellsNanotoxicology2011533263402084602157YoshiokaYKurodaEHiraiTTsutsumiYIshiiKJAllergic responses induced by the immunomodulatory effects of nanomaterials upon skin exposureFront Immunol20178101692826122158ThierseHJGamerdingerKJunkesCGuerreiroNWeltzienHUT cell receptor (TCR) interaction with haptens: metal ions as non-classical haptensToxicology200520921011071576702059SalvioniLGalbiatiECollicoVNegatively charged silver nanoparticles with potent antibacterial activity and reduced toxicity for pharmaceutical preparationsInt J Nanomedicine201712251725302840882260PendersJStolzoffMHickeyDJAnderssonMWebsterTJShape-dependent antibacterial effects of non-cytotoxic gold nanoparticlesInt J Nanomedicine201712245724682840881761WuJLiuWXueCToxicity and penetration of TiO2 nanoparticles in hairless mice and porcine skin after subchronic dermal exposureToxicol Lett20091911181950113762RyuHJSeongNWSoBJEvaluation of silica nanoparticle toxicity after topical exposure for 90 daysInt J Nanomedicine20149Suppl 212713663HolmesAMLimJStudierHRobertsMSVarying the morphology of silver nanoparticles results in differential toxicity against micro-organisms, HaCaT keratinocytes and affects skin depositionNanotoxicology20161010150315142763654464NicaIStanMPopaMInteraction of new-developed TiO2-based photocatalytic nanoparticles with pathogenic microorganisms and human dermal and pulmonary fibroblastsInt J Mol Sci201718224965KukuGCulhaMInvestigating the origins of toxic response in TiO2 nanoparticle-treated cellsNanomaterials (Basel)201774E832839824166Osmond-McleodMJOytamYRoweALong-term exposure to commercially available sunscreens containing nanoparticles of TiO2 and ZnO revealed no biological impact in a hairless mouse modelPart Fibre Toxicol20151314467AlsalhiMSDevanesanSAlfuraydiAAGreen synthesis of silver nanoparticles using Pimpinella anisum seeds: antimicrobial activity and cytotoxicity on human neonatal skin stromal cells and colon cancer cellsInt J Nanomedicine201611443944492766043868CarrolaJBastosVJarakIMetabolomics of silver nanoparticles toxicity in HaCaT cells: structure activity relationships and role of ionic silver and oxidative stressNanotoxicology2016108110511172714442569AvalosAHazaAIMoralesPManufactured silver nanoparticles of different sizes induced DNA strand breaks and oxidative DNA damage in hepatoma and leukaemia cells and in dermal and pulmonary fibroblastsFolia Biol (Praha)201561133422595830970GongCTaoGYangLSiO2 nanoparticles induce global genomic hypomethylation in HaCaT cellsBiochem Biophys Res Commun201039733974002050132171YuKNYoonTJMinai-TehraniAZinc oxide nanoparticle induced autophagic cell death and mitochondrial damage via reactive oxygen species generationToxicol In Vitro2013274118711952345896672AhamedMAlhadlaqHAAlamJKhanMAAliDAlarafiSIron oxide nanoparticle-induced oxidative stress and genotoxicity in human skin epithelial and lung epithelial cell linesCurr Pharm Des20131937668166902362153073NejdlLKudrJMoulickAPlatinum nanoparticles induce damage to DNA and inhibit DNA replicationPLoS One2017127e01807982870443674ChangJLeeCWAlsulimaniHHRole of fatty acid composites in the toxicity of titanium dioxide nanoparticles used in cosmetic productsJ Toxicol Sci20164145335422743223975VieiraLFLinsMPVianaIMdos SantosJESmaniottoSReisMDMetallic nanoparticles reduce the migration of human fibroblasts in vitroNanoscale Res Lett20171212002831436876SekerSEl inAEYumakTS na AEl inYMIn vitro cytotoxicity of hydrothermally synthesized ZnO nanoparticles on human periodontal ligament fibroblast and mouse dermal fibroblast cellsToxicol in Vitro2014288134913582501613477G ng ne CD ekerSEl inAEEl inYMA comparative study on the in vitro cytotoxic responses of two mammalian cell types to fullerenes, carbon nanotubes and iron oxide nanoparticlesDrug Chem Toxicol20174022152272742466678WeiXJiangWYuJDingLHuJJiangGEffects of SiO2 nanoparticles on phospholipid membrane integrity and fluidityJ Hazard Mater20152872172242566116879de PlanqueMRAghdaeiSRooseTMorganHElectrophysiological characterization of membrane disruption by nanoparticlesACS Nano201155359936062151708380WeiXYuJDingLHuJJiangWEffect of oxide nanoparticles on the morphology and fluidity of phospholipid membranes and the role of hydrogen bondsJ Environ Sci (China)2017572212302864724281AliASuhailMMathewSNanomaterial induced immune res ponses and cytotoxicityJ Nanosci Nanotechnol201616140572739843282KinodaJIshiharaMHattoriHNakamuraSFukudaKYokoeHCytotoxicity of silver nanoparticle and chitin-nanofiber sheet composites caused by oxidative stressNanomaterials (Basel)2016610E1892833531783HarvanovaMPJiravovaJMalohlavaJTomankovaKBJirovaDKolarovaHRaman imaging of cellular uptake and studies of silver nanoparticles effect in BJ human fibroblasts cell linesInt J Pharm20175281 22802862860280184SiddiquiMASaquibQAhamedMMolybdenum nanoparticles-induced cytotoxicity, oxidative stress, G2/M arrest, and DNA damage in mouse skin fibroblast cells (L929)Colloids Surf B Biointerfaces201512573812543706685MohamedMSTorabiAPauloseMKumarDSVargheseOKAnodically grown titania nanotube induced cytotoxicity has genotoxic originsSci Rep20177418442816549186NarendhirakannanRTHannahMAOxidative stress and skin cancer: an overviewIndian J Clin Biochem20132821101152442619587TuMHuangYLiHLGaoZHThe stress caused by nitrite with titanium dioxide nanoparticles under UVA irradiation in human keratinocyte cellToxicology2012299160682262729788TyagiNSrivastavaSKAroraSComparative analysis of the relative potential of silver, zinc-oxide and titanium-dioxide nanoparticles against UVB-induced DNA damage for the prevention of skin carcinogenesisCancer Lett2016383153612769363289PalAAlamSMittalSUVB irradiation-enhanced zinc oxide nanoparticles-induced DNA damage and cell death in mouse skinMutat Res Genet Toxicol Environ Mutagen201680715242754271190MagdolenovaZCollinsAKumarADhawanAStoneVDusinskaMMechanisms of genotoxicity: a review of in vitro and in vivo studies with engineered nanoparticlesNanotoxicology2014832332782337960391ZhuXHondroulisELiuWLiCZBiosensing approaches for rapid genotoxicity and cytotoxicity assays upon nanomaterial exposureSmall201399 10182118302341799992DissanayakeNCurrentKObareSMutagenic effects of iron oxide nanoparticles on biological cellsInt J Mol Sci2015161023482235162643739793GaoFMaNZhouHZinc oxide nanoparticles-induced epigenetic change and G2/M arrest are associated with apoptosis in human epidermal keratinocytesInt J Nanomedicine201611385938742757045394CostaPMFadeelBEmerging systems biology approaches in nanotoxicology: towards a mechanism-based understanding of nanomaterial hazard and riskToxicol Appl Pharmacol20162991011112672131095SierraMIVald sAFernandezAFTorrecillasRFragaMFThe effect of exposure to nanoparticles and nanomaterials on the mammalian epigenomeInt J Nanomedicine201611629763062793287896GongCTaoGYangLMethylation of PARP-1 promoter involved in the regulation of nano-SiO2-induced decrease of PARP-1 mRNA expressionToxicol Lett201220932642692226586897ShyamasundarSNgCTYungLYDheenSTBayBHEpigenetic mechanisms in nanomaterial-induced toxicityEpigenomics2015733954112607742898XuBMaoZJiXmiR-98 and its host gene Huwe1 target caspase-3 in silica nanoparticles-treated male germ cellsSci Rep20155129382626318399OuLSongBLiangHToxicity of graphene-family nanopar-ticles: a general review of the origins and mechanismsPart Fibre Toxicol20161315727799056100ZhaoYHoweJLYuZExposure to titanium dioxide nanoparticles induces autophagy in primary human keratinocytesSmall20139338739223090781101YuQWangHPengQLiYLiuZLiMDifferent toxicity of anatase and rutile TiO2 nanoparticles on macrophages: involvement of difference in affinity to proteins and phospholipidsJ Hazard Mater201733512513428437696102WuQJinRFengTIron oxide nanoparticles and induced autophagy in human monocytesInt J Nanomedicine2017123993400528603414103GaoXWangYPengSComparative toxicities of bismuth oxybromide and titanium dioxide exposure on human skin keratinocyte cellsChemosphere2015135839325917605104MeyerKRajanahalliPAhamedMRoweJJHongYZnO nanoparticles induce apoptosis in human dermal fibroblasts via p53 and p38 pathwaysToxicol In Vitro20112581721172621903158105SzmydRGoralczykAGSkalniakLEffect of silver nanoparticles on human primary keratinocytesBiol Chem2013394111312323091270106Leite-SilvaVRle LamerMSanchezWYThe effect of formulation on the penetration of coated and uncoated zinc oxide nanoparticles into the viable epidermis of human skin in vivoEur J Pharm Biopharm201384229730823454052107BurgstallerGSenguptaAVierkottenSDistinct niches within the extracellular matrix dictate fibroblast function in (cell free) 3D lung tissue culturesAm J Physiol Lung Cell Mol Physiol20183145L708L72329345200108CantiniMGomideKMoulisovaVGonz lez-Garc aCSalmer n-S nchezMVitronectin as a micromanager of cell response in material-driven fibronectin nanonetworksAdv Biosyst201719170004729497701109DenningDRoosWHElucidating the molecular mechanisms underlying cellular response to biophysical cues using synthetic biology approachesCell Adh Migr201610554055327266767110SadriehNWokovichAMGopeeNVLack of significant dermal penetration of titanium dioxide from sunscreen formulations containing nano- and submicron-size TiO2 particlesToxicol Sci2010115115616620156837111AlarifiSAliDAlkahtaniSOxidative stress-induced DNA damage by manganese dioxide nanoparticles in human neuronal cellsBiomed Res Int20172017547879028596964112ZhangJZouZWangBLysosomal deposition of copper oxide nanoparticles triggers HUVEC cells deathBiomaterials201816122823929421558113FilonFLCroseraMAdamiGBovenziMRossiFMainaGHuman skin penetration of gold nanoparticles through intact and damaged skinNanotoxicology20115449350121319954114Galand kov AFrankov JAmbro ov NEffects of silver nanoparticles on human dermal fibroblasts and epidermal keratino-cytesHum Exp Toxicol201635994695726500221Skin penetration of metal NPs.', 'Possible toxic mechanisms of metal NPs in HaCaT cells.']","Figure 1 Skin penetration of metal NPs. Three main possible skin-penetration pathways are illustrated: the intracellular pathway, intercellular pathway, and follicular pathway. Metal NPs may penetrate the stratum corneum in healthy skin. In damaged skin, more NPs may penetrate the epidermis and dermis. They may move to the lymph modes and be engulfed by macrophages. During penetration, metal NPs release metal ions, which induce metal ion-specific CD4 T-cell and IL17-mediated immunoreactions. NPs, nanoparticles.",yes
PMC2229649,Figure_3,oa_package/40/f1/PMC2229649.tar.gz,"['A two week induction period in 2 3 month old transgenics resulted in an approximate 40 loss in dopaminergic SN cell numbers compared to either untreated transgenics or wildtype littermates; this loss was prevented by deprenyl co-treatment (A).', 'Levels of S100- , a marker for activated astrocytes, were also increased in transgenic astroglia following dox induction (E).', 'g003Inducible elevation in astrocytic MAO-B results in selective, age-related SN dopaminergic neurodegeneration and global astrocyte activation even in the absence of neurotoxin.', 'g003""/>We performed silver staining to verify that selective loss of TH immunostained cells in the SN following dox induction coincided with increased neurodegeneration in this brain region (F).', 'Significantly, dopaminergic SN cell loss was found to be attenuated in the presence of co-treatment with not only deprenyl, but also EUK189 suggesting that oxidative stress is an important component of dopaminergic demise (A).', 'Co-treatment of animals with an inhibitor of microglial activation, minocycline, prevented dopaminergic SN cell death suggesting that microglial activation plays a major role in dopaminergic neurodegeneration in this model (A).']",10.1371/journal.pone.0001616.g003,yes
PMC9482454,Figure_4,oa_package/d7/30/PMC9482454.tar.gz,"['CA1 excitatory neurons that exhibit place selective activities can be identified from the populations across the genotypes and ages (A D).', 'We find that the calcium event rates across mouse groups do not exhibit significant differences (E), but AD mice show a significant difference from Non-Tg mice in terms of calcium event amplitudes (', 'Consistent with our finding in mouse exploration of open fields, we find that for both ages, 3xTg-AD mice exhibit lower spatial information score in bits/event than Non-Tg mice (G; the respective 50%-cumulative values for Non-Tg young (238 place cells in direction 1 + 246 place cells in direction 2, from 6 mice), 3xTg-AD young (213 place cells in direction 1 + 237 place cells in direction 2, from 8 mice), Non-Tg old (163 place cells in direction 1 + 159 place cells in direction 2, from 5 mice) and 3xTg-AD old are (259 place cells in direction 1 + 272 place cells in direction 2, from 5 mice) are 2.', 'During linear track trials, hippocampal CA1 excitatory neurons in old 3xTg-AD mice have higher sparsity values than young 3xTg-AD mice, while for control mice, there are no significant differences between ages (H; the respective 50%-cumulative values for Non-Tg young (1742 cells (each direction) from 6 mice), 3xTg-AD young (1988 cells (each direction) from 8 mice), Non-Tg old (1136 cells (each direction) from 5 mice) and 3xTg-AD old (1560 cells (each direction) from 6 mice) are 0.', '3xTg-AD mouse cells exhibit higher spatial coherence values than Non-Tg mice (I; the respective 50%-cumulative values for Non-Tg young (1742 cells (each direction) from 6 mice), 3xTg-AD young (1988 cells (each direction) from 8 mice), Non-Tg old (1136 cells (each direction) from 5 mice) and, 3xTg-AD old (1560 cells (each direction) from 6 mice) are 0.', '.']","Fig. 4. Linear track experiment data also support impaired spatial coding in 3xTg-AD hippocampal CA1. A-D. Example calcium event rate maps from mice running on a horizontal linear track with different genotypes and ages. Top: the black line represents the movement trajectory of mice. Superimposed red dots represent the locations where calcium events occur. Bottom: smoothed spatial rate maps. E. Violin plots of calcium event rates of all neurons from different groups of mice. Only the periods with movement speeds higher than 5 mm/s were used for event rate calculation, and event rates from trials with the same track direction were averaged. F. Violin plots of calcium event amplitudes of all neurons from different groups of mice. G. Cumulative distribution plots of spatial information scores (in bits/event) for all place cells from Non-Tg old, 3xTg-AD old, Non-Tg young and 3xTg-AD young mice, for both horizontal and vertical tracks in two directions. H and I. Cumulative distribution plots of sparsity (H) spatial coherence (I) values of all neurons for Non-Tg old, 3xTg-AD old, Non-Tg young and 3xTg-AD young mice, in both square and circle arena. LME analyses were used for E-F; two-sample Kolmogorov-Smirnov tests were used for G-I. *, ** and *** indicate the significance levels with the respective p values of <0.05, <0.005, and < 0.0005.",yes
PMC5596937,Figure_6,oa_package/17/0c/PMC5596937.tar.gz,"[' 6a-b).', '6c, e).', '6d, f) than patients with high INTS6 expression.', 'Kaplan-Meier analysis of overall and disease-free survival time in HCC patients (log-rank test, p = 0.', 'Kaplan-Meier analysis of overall survival and disease-free survival rates in subclassified HCC patients Stratified survival analysis of overall and disease-free survival rates according to poor pathology grades II III (c d) and tumour recurrence (e f)\nINTS6 was an independent predictor of overall and disease-free survival rates in HCC patientsUnivariate and multivariate Cox analyses explored the impacts of the expression of INTS6 and other clinicopathological parameters on the overall and disease-free survival rates in 70 archived paraffin-embedded HCC patient samples.']","Fig. 6 Kaplan-Meier analysis of overall and disease-free survival time in HCC patients (log-rank test, =0.001 and =0.001) ( ). Kaplan-Meier analysis of overall survival and disease-free survival rates in subclassified HCC patients Stratified survival analysis of overall and disease-free survival rates according to poor pathology grades IIIII ( ) and tumour recurrence ( )",yes
PMC7465814,Figure_3,oa_package/d9/e3/PMC7465814.tar.gz,"['Diagnosis and Management of Incident AFAs shown in A, the diagnosis of AF in the 266 patients took place in different settings.', 'As shown in B, most patients were prescribed an anticoagulant (any anticoagulant: n = 245 (92%); direct oral anticoagulant: n = 127; vitamin K antagonist: n = 110; low-molecular-weight heparin: n = 8).', ', previous MI and/or PCI 12 months) (C), an anticoagulant was used in 225 (94%), still often combined with an antiplatelet drug (anticoagulant alone: n = 121; anticoagulant + single-antiplatelet therapy: n = 102; anticoagulant + dual-antiplatelet therapy: n = 2).', 'Diagnostic circumstances and antithrombotic management of incident atrial fibrillation (AF) in coronary artery disease (CAD) outpatients.']","Figure 3 Diagnostic circumstances and antithrombotic management of incident atrial fibrillation (AF) in coronary artery disease (CAD) outpatients. ( ) Diagnostic circumstances of incident atrial fibrillation (AF) in coronary artery disease (CAD) outpatients. HF, heart failure; MI, myocardial infarction ( ) Antithrombotic strategy in all coronary artery disease (CAD) outpatients with incident atrial fibrillation (AF); ACT, anticoagulant therapy; DAPT, dual-antiplatelet therapy; SAPT, single-antiplatelet therapy. ( ) Antithrombotic strategy in patients with incident AF in a context of chronic CAD (i.e., patients without recent (<1 year) history of myocardial infarction and/or percutaneous coronary intervention).",yes
PMC7732016,Figure_2,oa_package/34/6b/PMC7732016.tar.gz,"['Contrast computed tomography scan at admission showing a pelvic spleen with whorled appearance of vessels (black arrow)Considering the long history of complaints and the radiological observations, the patient was managed conservatively and remained in the hospital for 7 days.']",Figure 2 Contrast computed tomography scan at admission showing a pelvic spleen with whorled appearance of vessels (black arrow),yes
PMC5899844,Figure_1,oa_package/44/ef/PMC5899844.tar.gz,['IJO_983_1628820163(a) Clinical aspect of the tumor.'],Figure 1 (a) Clinical aspect of the tumor. (b) Ultrasound examination.,yes
PMC4206811,Figure_7,oa_package/56/51/PMC4206811.tar.gz,"['3) Among 9 patients with C4-5 synostosis, two (25%) out of 9 patients had spondylosis at the cephalad C3-4 segment, while 2 had spondylosis at caudate C5-6 (2) and C6-7 (1) ().', 'Lateral radiograms of 3 patients with congenital C4-5 synostosis (A-C), aged from 56 to 90 years, showing a slightly inwaisted fused vertebral body with some caudal corporal flaring.']","Fig. 7 Lateral radiograms of 3 patients with congenital C4-5 synostosis , aged from 56 to 90 years, showing a slightly inwaisted fused vertebral body with some caudal corporal flaring. In all 3 patients, spondylotic changes were observed at the proximal and caudal discs, respectively.",yes
PMC5597217,Figure_2,oa_package/5b/c5/PMC5597217.tar.gz,"['squamipes exhibited concomitant occurrence of granulomas in different stages of maturation: PE, NE, EP and P ().', 'Indeed, at chronic phase (120 days of infection) of the experimental infection, the four evolutionary stages of granulomas (PE, NE, PE and P) were clearly observed in infected mice ().', 'g002Representative types of granulomas and their frequencies in target organs of rodents naturally or experimentally infected with S.', '0001), ().', '0001) ().', 'squamipes and only granulomas EP (100%) for Swiss mice (acute and chronic phases) ().', ' shows the granuloma types and their respective frequencies in all three groups studied.', 'Our quantitative data revealed a prevalence of granulomas with necrotic-exudative features (~80%) in the liver during the acute phase of schistosomiasis mansoni in mice ().', 'In fact, in the liver of chronically infected mice, we found predominance of EP and P granulomas, which are in late evolutional phases, characterized by the presence of collagen fibers and lower number of cells around the egg ().', 'squamipes (), WSI revealed granulomas similar in type and frequency to those found in the chronic phase of Swiss mice experimentally infected.', 'In the small and large intestines of both infected models, WSI showed granulomas with few variations in size and evolutionary phases, with predominance of EP type ().']",10.1371/journal.pone.0184696.g002,yes
PMC3387014,Figure_3,oa_package/c0/16/PMC3387014.tar.gz,"['A shows a representative time-lapse sequence photographed by combined confocal and bright-field imaging over 72 hours after infection.', 'g003Distribution of C.', 'albicans cells were mainly observed inside granulomas, where they appeared to be associated with the core (B and (a) USO material inserted into endobag.']",Figure 3 (a) USO material inserted into endobag. (b) Specimen extraction using endobag.,yes
PMC5000510,Figure_1,oa_package/1b/cf/PMC5000510.tar.gz,"[' 1.', '', ' 1Main stages required to perform IB-CFD simulation in generalThe geometry-prescribed method is based on the assumption that the flow-induced load on the LV wall is negligible in comparison to the structural-induced load on the fluid flow [36].']",Fig.1 Main stages required to perform IB-CFD simulation in general,yes
PMC3170270,Figure_9,oa_package/f3/8b/PMC3170270.tar.gz,"['""Patient 1 - Anteroposterior and lateral preoperative radiographs.', '"" 2-year postoperative correction anteroposterior and lateral X-rays of the patient in .', '"" 2-year postoperative correction anteroposterior and lateral X-rays of the patient in .']","Figure 9 ."" Patient 1 - Anteroposterior and lateral radiographs show a 52 main thoracic curve and a 38 upper thoracic curve, with 24 dorsolumbar kyphosis. MIS technique was utilized (Figure 10 & 11), and both of the curves were instrumented.",yes
PMC7666854,Figure_2,oa_package/d7/5f/PMC7666854.tar.gz,[],"Figura 2 Linfoma de Burkitt. Infiltrado tumoral. Hematoxilina-eosina, 10X. Inmunohistoqumica: CD20, 20X. Inmunohistoqumica: Ki67,20X",yes
PMC3114860,Figure_4,oa_package/c8/e8/PMC3114860.tar.gz,"['As shown in figure 4, histological examination of lungs from infected mice by routine H E staining at day 7 post infection revealed various size foci of pneumonia.', 'Overall, the TLR2-deficient mice had more severe neutrophilic infiltration while the wild type C57BL/6 mice displayed more prominent fibroblast proliferation, suggesting superior repair (\nA, 4B\n).', 'Consistent with this observation, only lung tissue from the wild type mice stained for the intermediate filament vimentin at 5 days post infection (\nE, 4F\n), although by day 14 vimentin staining was similar in both mouse strains (data not shown).', 'By 14 days post infection, inflammation was still visible in the TLR2-deficient mice while inflammation was largely resolved in the wild type mice (\nC, 4D\n).', 'Moreover, in more than half of the TLR2-deficient mice, we observed prominent, well-developed bronchus associated lymphoid tissue (BALT) containing CD19-expressing B lymphocytes at 5 days post infection, consistent with inducible BALT (iBALT) (\nG, 4H\n).', 'g004Intranasal infection with C.']",10.1371/journal.pone.0020846.g004,yes
PMC7327467,Figure_3,oa_package/fc/03/PMC7327467.tar.gz,"['Radiology workers must don PPE that fully covers all body surfaces (A) and then prepare for operation of the imaging equipment.', 'The recommendation is to guide patients to the appropriate position on the examination bed by intercom (B).', 'This cleaning is accomplished at least twice a day for a period of 30 min (C).', 'Table 1Personal protective equipment for health care personnel in a room suspected or confirmed to be positive for COVID-19.']","Fig. 3 A, photograph of personal protective equipment for Level II radiologic technician protection in a CT scanner room. B, technician in a console room during CT examination of a patient infected with SARS-CoV-2. C, radiological technician wiping the surface of a CT table with disinfectant.",yes
PMC9648008,Figure_2,oa_package/25/76/PMC9648008.tar.gz,"['sIL-2R, MMP-2, and MMP-9 in patients with IOL with and without extraocular involvement.', '05; Mann Whitney U testThe results of the comparisons among 3 groups; IOL without extraocular involvement group, IOL with extraocular involvement group, and uveitis group are shown in Supplementary ']","Fig. 2 sIL-2R, MMP-2, and MMP-9 in patients with IOL with and without extraocular involvement. Serum sIL-2R ( ), and vitreous sIL-2R ( ), MMP-2 ( ), and MMP-9 ( ) in patients with IOL with and without extraocular involvement. * <0.05; MannWhitney U test",yes
PMC10399646,Figure_2,oa_package/49/10/PMC10399646.tar.gz,"[', 1982) ().', 'The dog prostatic artery, which arises from the internal pudendal artery (branch of the iliac internal artery), is the arterial supply of blood to the dog prostate () (Budras et al.', 'Arteries, veins, and nerve trunks can be recognized in the posterolateral region of the prostate within loose connective and adipose tissue (Kiyoshima, 2004) ().', '.']","Fig. 2. Arterial supply (red) and venous drainage (blue) in human (A), rat (B) and dog (C) prostate gland. The figure was partly generated using Servier Medical Art, provided by Servier, licensed under a Creative Commons Attribution 3.0 unported license.",yes
PMC7593615,Figure_3,oa_package/df/d7/PMC7593615.tar.gz,"['In both groups, with definite/likely or with possible diagnosis, there was a trend for larger height-adjusted liver volumes in women, but the difference was not statistically significant (', 'The numbers of hepatic cysts in male and female patients with possible ADPLD were also similar (', '']","Fig.2 Representative abdominal or CT images from patients with possible ADPLD. (A) A 49-yr-old woman; liver volume 1,287 ml. (B) A 64-yr-old woman; liver volume 1,642 ml. (C) A 77-yr-old woman; liver volume 3,502 ml. (D) A 51-yr-old man; liver volume 1,778 ml. (E) An 80-yr-old man; liver volume 1,385 ml. (F) An 81-yr-old man; liver volume 1,547 ml. (G) A 77-yr-old female; live volume 1,623 ml. ADPLD, autosomal-dominant polycystic liver disease; CT, computed tomography.",yes
PMC3412694,Figure_1,oa_package/8f/f9/PMC3412694.tar.gz,['Construction of plasmids for PPRV rescue.'],"Figure 1 ( ) The cDNA fragments F1, F2, F3 and F4 were reverse transcribed and amplified from PPRV/N75/1 genomic RNA. The hammerhead ribozyme sequence (HamRz) and the hepatitis delta virus ribozyme sequence (HdvRz) were introduced to the 5 end of F1 and the 3 end of F4, respectively. All fragments were then subcloned stepwise into the pCI vector to produce plasmid pN75/1. ( ) DNA fragments Fa (from the HamRz to the GS sequence of M with a site introduced at 3 end) and Fb (from GE of P gene to the end of F1 with and I sites introduced at the 5 end) were PCR-amplified from pN75/1 and ligated together to get fragment Fab, then section F1 of pN75/1 was replaced with Fab to get plasmid pN75/1-insertion. The net result was equal to insertion of a morbillivirus gene start (GS) sequence, I and I sites, gene end (GE) sequence and CTT intergenic trinucleotide into pN75/1 between nt 3405 and 3406 of the PPRV/N75/1 genome cDNA sequence. ( ) The GFP ORF with a Kozak sequence at the 5 end of the ORF was inserted into plasmid pN75/1-insertion to produce plasmid pN75/1-GFP.",yes
PMC9394429,Figure_6,oa_package/e1/02/PMC9394429.tar.gz,[],"Figure6 Similar tubular injury and collapse of glomerular capillary tuft in kidneys of WT and SBP-1 KO infected mice. C57BL/6 mice were infected with WT and SBP-1 KO NK65 parasites and at day 7 and 8 p.i. kidneys were dissected. Sections were stained with PAS. Representative images are shown (original magnification x40, bar = 50 m). Red frames indicate collapse of glomerular tufts. Black arrowheads indicate vacuolization of proximal tubular epithelial cells, red arrowheads indicate loss of brush border of proximal tubular epithelial cells. Percentage of glomeruli with collapsed glomerular tufts counted on PAS stained kidney sections Asterisks above data points indicate significant differences compared to control mice, *p < 0.05. Data of two experiments, Control: n = 6, WT d7-d8: n = 5-6, SBP-1 KO d7-d8: n = 5. Mann-Whitney U test with Holm-Bonferroni correction for multiple testing (number of tests = 6) was performed in panel B.",yes
PMC10784209,Figure_1,oa_package/30/10/PMC10784209.tar.gz,"['The four major HIBI subtype classifications in this study:The central or Rolandic basal ganglia-thalamus (RBGT) subtype, which includes patients with isolated high metabolic area injury (sensorimotor cortex, ventral posterior lateral (VPL) nucleus of the thalamus, posterior putamen and hippocampus), is subdivided into four gradations of injury, based on the degree of destruction of the parasagittal cortex19 ().']","FIGURE 1 Axial FLAIR images in patients with the Rolandic basal ganglia-thalamus subtype demonstrating the classification of the four subgroups of paramedian extension of perirolandic injury, (a) Mild, (b) Moderate, (c) Severe, (d) MPI.",yes
PMC6963120,Figure_3,oa_package/1d/dd/PMC6963120.tar.gz,"['However, the mRNA levels of -SMA and vimentin did not reach statistical significance ( 3A).', ' 3Expression of HIF-1 in cavernous tissue.', 'However, the mRNA levels of p38 did not reach statistical significance ( 3B).', '14) ( 3C and D).']","Figure3 Expression of HIF-1 in cavernous tissue. In Panels A and B, there were no significant differences in -SMA, vimentin, ERK, JNK, and p38 between both groups. In Panel C, the result of HIF-1 was presented by immunohistochemical staining in both groups. Strong expression of HIF-1 was detected in CNCI group. In Panel D, the results of western blot show that HIF-1 increased significantly in CNCI groups compared with sham group (mean standard deviation) (magnification200). Statistically significant (independent test): HIF-1 ( = .047). -SMA= -smooth muscle actin; CNCI= cavernous nerve crush injury; ERK, extracellular signal-regulated kinase; HIF-1= hypoxia-inducible factor-1; JNK= c-Jun NH -terminal kinase.",yes
PMC2841647,Figure_2,oa_package/16/43/PMC2841647.tar.gz,"['We therefore used both sequence and structure of region III as a basis for the design of cyclotraxin-B, which has been cyclized via terminal cysteine residues to mimic the native structure of this region in BDNF (See \nA\n).', 'g002Cyclotraxin-B is a highly potent allosteric inhibitor of TrkB receptor with long-lasting effects.', '07 nM; \nB,D\n).', '0009777-OLeary1"" ref-type=""bibr"">[22], cyclotraxin-B proved to have a potency three-order of magnitude higher, in KIRA-ELISA (L2-8 IC50 = 108 63 nM; \nB,D\n).', '9 pM), while still remaining non-competitive (\nC,E\n\nand .']","Figure 2. Hematoxylin and eosin stain of the skin lesions revealing a dense infiltrate of monotonous neoplastic cells with dispersed chromatin and a high nuclear-to-cytoplasmic ratio, consistent with leukemia cutis (a) 10 and (b) 40.",yes
PMC4741915,Figure_5,oa_package/02/85/PMC4741915.tar.gz,['Intracellular mechanism of action of Reg1 in the inhibition of ISC activationA.'],"Figure 5 Intracellular mechanism of action of Reg1 in the inhibition of ISC activation Western blotting of ISCs by Erk1/2, P-Erk1/2, Akt, P-Akt, Smad2/3, P-Smad2/3, Smad7, TGF- antibody. Data are expressed as mean SE (n = 3), * < 0.05, ** < 0.01. Western blotting of db/db ISCs treated with rhReg1, U0126 (10uM), LY-294002 (10uM), SB431542 (10uM), sh-Reg1, sh-EXLT3 and sh-EXTL3+Reg1 by Erk1/2, P-Erk1/2, Akt, P-Akt, Smad2/3, P-Smad2/3 and Smad7 antibody.",yes
PMC8784181,Figure_4,oa_package/bc/2b/PMC8784181.tar.gz,"['A CT head following her acute presentation showed no intracranial event ().', '\n\nCT of the head (left: sagittal bone and soft tissue windows, and, right: axial bone window and soft tissue window at the same levels 2) showing sphenoid mucopyocele, retroclival dural thickening and increasing clival dehiscence (anterior and posterior cortex).']","Figure 4 CT of the head (left: sagittal bone and soft tissue windows, and, right: axial bone window and soft tissue window at the same levels 2) showing sphenoid mucopyocele, retroclival dural thickening and increasing clival dehiscence (anterior and posterior cortex).",yes
PMC9600253,Figure_3,oa_package/31/77/PMC9600253.tar.gz,"['Case 2: The hysterectomy specimen incidentally showed an ECP (A).', 'A 19 mm elongated polyp arising in the endocervix had a 3-mm exophytic tumor protruding from the surface (B).', 'Histologically, the EC involving ECP consisted predominantly of solid cellular sheets showing severe nuclear pleomorphism (grade 3 EC; C), intermingled with mature squamous morules (squamous differentiation; D).', 'In contrast, the endometrial tumor was EC of grade 1 with mucinous differentiation (E,F), measuring 20 mm at its largest dimension and 14 mm at its deepest invasion depth.', 'Additionally, the patient had an ovarian mass, which was histologically compatible with grade 3 EC (G).', 'The presence of predominantly solid architecture (H) and scattered areas of squamous differentiation (I) was similar to the EC involving ECP.', 'Immunohistochemical staining revealed that the EC involving ECP was negative for ER (J) and PR (K) but diffusely and strongly positive for p16 (L).', 'The immunophenotypes of the ovarian EC were identical to those of the EC involving ECP: a complete absence of ER (M) and PR (N) expression and uniform p16 positivity with strong staining intensity (O).', 'WT1 negativity in the ovarian tumor excluded high-grade serous carcinoma (P).', 'In contrast, endometrial EC demonstrated diffuse and strong nuclear expression for ER (Q) and PR (R).', 'Patchy p16 positivity in the endometrial EC (S) was different from intense and uniform p16 expression in the ovary and ECP.', 'No remarkable lesion was identified in the cervix (K).', 'Grade 3 ovarian EC involving ECP: case 2.']","Figure 3 Grade 3 ovarian EC involving ECP: case 2. ( ) The hysterectomy specimen shows an elongated ECP. EC (blue arrow) appears as an exophytic mass protruding from the surface of ECP. ( ) EC involving ECP ( ) superficially invades the stroma of the polyp up to 0.3 mm, ( ) consists predominantly of solid sheets of tumor cells, and ( ) exhibits severe nuclear pleomorphism, coarse chromatin, and prominent nucleoli. Foci of squamous differentiation are readily identifiable. ( ) In the endometrial EC, well-differentiated glands are confluent and crowded. ( ) Areas of mucinous differentiation are noted. ( ) The ovarian EC demonstrates a diffuse infiltrative growth pattern with foci of geographic tumor necrosis. ( ) The solid architecture occupies more than half of the tumor, compatible with grade 3 EC. ( ) High-grade nuclear atypia and the presence of squamous differentiation are the same as those of EC involving ECP. ( ) Immunohistochemically, EC involving ECP is negative for ( ) estrogen receptor (ER) and ( ) progesterone receptor (PR) and ( ) positive for p16 with strong staining intensity. Ovarian EC is also negative for ( ) ER and ( ) PR and ( ) uniformly and intensely positive for p16. ( ) WT1 negativity excludes the possibility of serous carcinoma. ( ) Endometrial EC shows strongly positive expression for ( ) ER and ( ) PR and ( ) patchy p16 positivity. ( ), Hematoxylin and eosin staining. ( ), immunohistochemical staining using polymer method. Original magnification: ( ), 10; ( ), 20; ( ), 150; ( ), 200; ( ), 25; ( ), 200; ( ), 25; ( ), 100; ( ), 400; ( ), 150; ( ), 150; ( ), 30; ( ), 100; ( ), 100; ( ), 100; ( ), 100; ( ), 100; ( ), 100; ( ), 100.",yes
PMC8628473,Figure_2,oa_package/0a/8e/PMC8628473.tar.gz,"['2A, B, high transfection efficiencies and specificities of three circVAPA-specific shRNAs were confirmed in NSCLC cells.', '2C).', '2D), further demonstrating that circVAPA knockdown restrained the proliferation of NSCLC cells.', '2E).', '2F), further demonstrating that circVAPA interference suppressed the proliferation and induced the apoptosis of NSCLC cells.', 'Knockdown of circVAPA inhibits the proliferation and induces the apoptosis of NSCLC cells.', '001CircVAPA silencing restrains the migration and invasion of NSCLC cellsThe migration and invasion abilities of tumor cells were highly associated with tumor invasiveness.']","Fig. 2 Knockdown of circVAPA inhibits the proliferation and induces the apoptosis of NSCLC cells. , The expression levels of circVAPA and VAPA were examined by RT-qPCR assay in A549 and H1299 cells transfected with sh-NC, sh-circVAPA#1, sh-circVAPA#2, or sh-circVAPA#3. The cell viability of A549 and H1299 cells was measured by MTT assay. Colony-forming ability of A549 and H1299 cells was assessed by colony-forming assay. The flow cytometry assay was performed to measure cell apoptosis. Western blot assay was conducted to measure the expression of proliferation-associated protein (Ki-67) and pro-apoptotic protein (Bax). ** < 0.01, *** < 0.001",yes
PMC6352390,Figure_2,oa_package/62/4e/PMC6352390.tar.gz,"[' 2), dementia or stroke, ASL has remained underused.', 'a c Example of a 24-year-old female patient, with oligodendroglioma (grade II) in the left frontal lobe.', 'Bisdas)Challenge 2: Developing new disease-specific, targeted and image-guided therapiesBiomedical imaging is an indispensable tool in personalised medicine providing information on localisation, extent, homogeneity and aggressiveness of diseaseDiagnostic imaging procedures are increasingly used to support individualised and targeted treatment and represent a mainstay of the progress of personalised medicine [10].']","Fig. 2 Example of a 24-year-old female patient, with oligodendroglioma (grade II) in the left frontal lobe. Cerebral blood flow, imaged by ASL. Red indicates high blood flow. T1-Gd and FLAIR. Example of a 63-year-old female patient with GBM (grade IV) in the right temporal lobe. Cerebral blood flow, imaged by ASL. Red indicates high blood flow. T1 post Gd. FLAIR. A clear difference in tumour blood flow can be seen between both patients (courtesy: A. Alsaedi, S. Bisdas)",yes
PMC6667607,Figure_9,oa_package/74/a4/PMC6667607.tar.gz,"[' 9).', 'Bipartite medial cuneiform.', 'c Sagittal CT reconstruction shows that the addition of the volume of the two bones is larger than a normal medial cuneiform would beThe common differential will be a fracture through the medial cuneiform.']","Fig. 9 Bipartite medial cuneiform. A 32-year-old man, on work up for left flat foot. Bilateral foot radiographs were taken. Left lateral view demonstrates a bipartite cuneiform (finding was bilateral but only right foot shown). The black arrow marks the synchondrosis in between medial cuneiform components. CT coronal reconstructions show a slightly larger plantar cuneiform. Sagittal CT reconstruction shows that the addition of the volume of the two bones is larger than a normal medial cuneiform would be",yes
PMC2688751,Figure_5,oa_package/74/3c/PMC2688751.tar.gz,"['Accordingly, in these preterm lambs, migration of gammadelta T-cells from the lower to the upper mucosa occurred sporadically after 2 or 14d post endotoxin treatment (A B).', 'In saline injected animals of 140d GA, gammadelta T-cells were also undetectable in the lamina propria (C).', 'However, in these near term lambs, the lymphoid follicles in the lower mucosa became populated with gammadelta T-cells (D).', 'Interestingly, 30 d of intraamniotic endotoxin exposure in near term animals resulted in migration of gammadelta T-cells from the lower to the upper mucosa in close proximity to areas of damaged intestinal epithelial cells (E).', 'g005Endotoxin induced chorioamnionitis results in migration of gammadelta T-cells to sites of mucosal damage at late GA.']",10.1371/journal.pone.0005837.g005,yes
PMC11426651,Figure_3,oa_package/9f/97/PMC11426651.tar.gz,"['jpbs_449_24-f003"">High power (x400) microscopic view of the lesion showing a closer view of spindle cells lacking cytological atypia, mitoses, and necrosisUpon follow-up after 2 weeks, the patient was doing well with no active complaints.']","Figure 3 High power (x400) microscopic view of the lesion showing a closer view of spindle cells lacking cytological atypia, mitoses, and necrosis",yes
PMC3391890,Figure_1,oa_package/ee/e2/PMC3391890.tar.gz,"['Volumetric reconstruction of the Thoracic computed tomography-scan at admission, showing multifocal areas of branched centrilobular nodules and patchy ground-glass opacities with few lobular reticulates (left).']","Fig. 1 Volumetric reconstruction of the Thoracic computed tomography-scan at admission, showing multifocal areas of branched centrilobular nodules and patchy ground-glass opacities with few lobular reticulates (left). At right a single 5-mm thick slice of the upper right lobe.",yes
PMC5636840,Figure_3,oa_package/ca/3d/PMC5636840.tar.gz,"[' 3a).', 'Progressive axonal transport defects in ALS patient-derived MNs.', 'Data values represent mean SEM\nAlthough the total number of mitochondria was not different between patient and control MNs at 6 to 7 weeks after initiation of differentiation (', ' 3b), patient-derived MNs showed a significant decrease in the number of moving mitochondria (', ' 3c).', ' 3e).', ' 3d), the number of moving mitochondria relative to the total number remained significantly lower in MNs derived from patient iPSCs compared with the controls (', ' 3e).', ' 3f).', ' 3g i).', ' 3i).', ' 3h).', ' 3i) shows that ER vesicle transport was also decreased, similar to what was observed for mitochondrial transport.', ' 3g).', ' 3e), PC release decreased with prolonged time in culture (Supplementary ']","Fig. 3 Progressive axonal transport defects in ALS patient-derived MNs. Representative fluorescent micrograph from control and FUS-ALS patient-derived MNs loaded with MitoTracker-Red (left) and typical kymographs (control: 3/3; patient: 3/1) obtained from MNs (right). Stationary mitochondria are visible as straight vertical lines, while moving mitochondria are deflected as tilted lines. Scale bar time (vertical): 35s; scale bar distance (horizontal): 25m. Quantification of the number of stationary mitochondria normalized to a neurite length of 100m during 200s for MNs derived from different iPSC lines at sixth to seventh week after starting MN differentiation ( =5). Data values represent meanSEM. Quantification of the number of moving mitochondria for MNs derived from different iPSC lines. Quantification of stationary mitochondria for motor neurons derived from a patient with a P525L mutation and a control iPSC line as a function of time after plating (2 weeks, 3 weeks, 4 weeks after plating, =13 and =14 for patient and control, respectively). Data values represent meanSEM. Quantification of moving mitochondria and ratio of moving to total mitochondria for motor neurons derived from a patient line (3/1) with a P525L mutation and a control iPSC line (3/2). EM analysis of control (3/2) and patient (R521H: 2/2; P525L:3/1)-derived motor neurons at the fourth week after plating. Images of both soma and neurites are shown. Representative images from three independent experiments are presented. Scale bar=100nm. Typical kymographs (control 1: 3/2; control 2: 3/3; R521H: 1/2; P525L: 3/1) obtained from motor neurons loaded with ER Tracker-Red. Scale bar time (vertical): 35s; scale bar distance (horizontal): 25m. Quantification of stationary ER vesicles from MNs derived from patients with P525L or R521H mutations and control iPSC lines at the fourth week after plating ( =10, =11, =12, =11 for Ctr1, Ctr2, R521H, and P525L, respectively). Data values represent meanSEM. Quantification of moving ER vesicles and ratio of moving to total ER vesicles for MNs derived from patients with P525L or R521H mutations and control iPSC lines (data are plotted as meanSEM; one-way ANOVA with post-hoc Tukeys test, *, **, *** values of 0.05, 0.01, and 0.001, respectively). Data values represent meanSEM",yes
PMC9643684,Figure_4,oa_package/06/9c/PMC9643684.tar.gz,"['No difference in the expression of KLRG1 in SG NK cells was observed between control and pSS model mice in the terms of proportion, cell number, and geometric mean fluorescence intensity (gMFI) (A).', 'Although no changes in the proportion and number of TRAIL+ SG NK cells were found in control and pSS model mice at 12 weeks of age, the gMFI of TRAIL expression in SG NK cells of pSS model mice was significantly downregulated compared with that of control mice (B).', 'The number of activated DR5+ CD44high CD4+T cells in the SGs from pSS model mice was significantly higher than that in the SGs from control mice, whereas no changes in the proportion and gMFI of activated DR5+ CD44high CD4+T cells were observed between control and SS model mice (C).']","FIGURE 4 Cytotoxic signature of SG NK cells against T cells in pSS model mice. The number (left) and geometric mean fluorescence intensity (gMFI) (right) of KLRG1 SG NK cells were evaluated by flow cytometric analysis. Data are presented as the means SD with five or six mice from each group. The number (left) and gMFI (right) of TRAIL SG NK cells were evaluated by flow cytometric analysis. Data are presented as the means SD with five or six mice from each group. ** < 0.0005 (two-tailed Students -test), vs. control. The number (left) and gMFI (right) of DR5 SG T cells were evaluated by flow cytometric analysis. Data are presented as the means SD with five or six mice from each group. * < 0.005 (two-tailed Students -test), vs. control.",yes
PMC9358021,Figure_7,oa_package/ad/b5/PMC9358021.tar.gz,"['As shown in A a clear, although small, CSFE-labeled cell population was present within the hydrogel, corroborating the findings (s 7C,D) on the ability of the extravasated NK cells to infiltrate the 3D tumor culture.', 'As a further demonstration, CD45 staining confirmed that all and only the CFSE-positive cells were belonging to the immunity lineage (B).']","FIGURE 7 Over/night dynamic co-culture of alginate-embedded neuroblastoma cells with circulating NK cells. Representative experiment showing a flow cytometry analysis of the cells recovered from the gels. A clear population of CFSE-labelled cells (corresponding to NK cells) is visible in the co-cultured sample ( = 6). Perfect concordance between CFSE-labelling and CD45 expression ( = 6). Confocal Analysis of different 3D alginate scaffold isolated from 3D dynamic cultures (4h). Representative confocal microscopy xy fields (370 370 micron) acquired in 3D alginate scaffolds containing GD2-positive NB cells (red) that were previously embedded in the gel, and subjected to 4h of dynamic flow of CFSE-labeled NK cells (green). Merged fluorescence images show that NK cells were able to infiltrate the gel. Scale bar is 50 micron.",yes
PMC4644744,Figure_11,oa_package/05/c4/PMC4644744.tar.gz,[],"Fig. 11 Dilated duodenum secondary to superior mesenteric artery (SMA) syndrome. On axial T2-weighted MR image, 2nd portion of duodenum is dilated and measures 4 cm (arrow). On sagittal image , it is detected that angle between aorta and SMA is narrowed (SMA syndrome). SMA syndrome can be diagnosed in clinically suspected cases on MRI by showing that aortomesenteric angle and distance are less than normal in reconstructed images and by dilatation proximal to obstruction. In barium studies, dilatation in duodenum, barium retention and vertical vascular external impression in 3rd segment are positive signs for SMA syndrome. However, these radiographic symptoms are non-specific, and they may also be seen in diseases such as scleroderma, diabetes, pancreatitis, and peptic ulcer.",yes
PMC8635164,Figure_1,oa_package/77/8d/PMC8635164.tar.gz,"['SS1"">The Neurovascular UnitThe cell types that constitute the NVU (depicted in ) and collaborate to perform its functions are endothelial cells (ECs), pericytes, smooth muscle cells (SMCs), astrocytes, and microglia (Iadecola, 2017; Freitas-Andrade et al.', 'We thank Ashley Carey for conceptualizing and drawing of the review.']","FIGURE 1 The neurovascular unit. Drawing depicting the cell types that make up the NVU within brain capillaries. Brain capillaries are surrounded by pericytes, as shown in this figure, while arteries and arterioles are surrounded by SMCs. Other important cells associated with blood vessels and important for BBB and neurovascular functions, also represented in this drawing, are astrocytes and microglia. ECs, endothelial cells; SMCs, smooth muscle cells; BBB, blood brain barrier; NVU, neurovascular unit; MCs, microglia cells, BM, basement membrane.",yes
PMC10167433,Figure_11,oa_package/16/6b/PMC10167433.tar.gz,[],"Figure 11 Keyword analysis of quantitative MRI and IDD-related research. (A) Visual map of keyword clustering analysis in this field. (B) Visual map of keyword timeline view analysis in this field. (C) Top 14 keywords with the strongest citation bursts in the WOSCC database. Red represents at high degree of keyword emergence during this period, while green represents a low degree of emergence. MRI, magnetic resonance imaging; IDD, intervertebral disc degeneration; WOSCC, Web of Science core collection.",yes
PMC10285468,Figure_3,oa_package/21/24/PMC10285468.tar.gz,"['Her right-sided keloid has been quiescent for 8 months ().', 'Keloids.']",Fig 3 Keloids. Patients and after treatment with dupilumab.,yes
PMC9308435,Figure_1,oa_package/86/01/PMC9308435.tar.gz,[],"FIGURE 1 Lateral radiographs (all in left lateral recumbency) of Cases 1 through 4 showing diffuse mild unstructured interstitial pattern throughout the pulmonary parenchyma. (A) Case 1 has a generalized unstructured interstitial pattern throughout the lungs, more conspicuous in the caudodorsal lung fields and superimposed with the cardiac silhouette. There is a microchip within the lateral thoracic soft tissues, superimposed with the cranial thorax. (B) Case 2 generalized unstructured interstitial pattern in a brachycephalic patient. The patient's thoracic cavity is shortened and cardiac silhouette appears widened because of breedrelated conformation. There is evidence of a sliding hiatal hernia in caudodorsal thorax (asterisk), as well as mild gas dilation of the mid thoracic esophagus (carets). There are also caudal thoracic vertebral malformations, considered an incidental breedrelated finding. (C) Case 3 generalized unstructured interstitial pattern throughout the lungs, more conspicuous in the caudodorsal lung fields. (D) Case 4 diffuse mild bronchial and unstructured interstitial pulmonary pattern, and ventrally dependent, focal, marked unstructured interstitial pattern (arrowheads) superimposed with the heart apex",yes
PMC9783654,Figure_4,oa_package/57/5c/PMC9783654.tar.gz,"['Next, we examined the efficiency of lentivirus siHIF-1 to downregulate HIF-1 and found out that the HIF-1 level in siHIF-1 -treated hypoxia group was significantly decreased compared to control and hypoxia groups without siHIF-1 treatment (a,b).', 'Interestingly, the results from the Western blotting showed that siHIF-1 attenuated hypoxia-induced tau hyperphosphorylation and had no effect on the level of total tau (a,c,d).', 'Intriguingly, downregulation of HIF-1 recovered the levels of the decreased PP2Ac methylation (e,f) and LCMT1 (e,h), while it had no effect on the total PP2Ac and PME-1 (e,g,i).', 'The result showed that hypoxia treatment accompanied with 50 mol/L 2ME2 dramatically suppressed the increased expression of HIF-1 induced by hypoxia (j,k).', 'It also suppressed tau phosphorylated at pS396 induced by hypoxia, without changing the total tau (j,l).', 'Inhibitor HIF-1 by 2ME2 increased the level of M-PP2Ac ( j,m) and LCMT1 (j,n).', 'Inhibition of HIF-1 expression reduces tau phosphorylation levels and reactivates PP2A in primary neurons.']","Figure 4 Inhibition of HIF-1 expression reduces tau phosphorylation levels and reactivates PP2A in primary neurons. ( ) Western blots for HIF-1 and tau phosphorylation levels at pS199, pS262, pS396, pS404 sites, and tau5 in primary neurons. ( ) Quantification of the relative protein expression levels (HIF-1, pS199, pS262, pS396, pS404, and tau5) after normalization to the -actin signal. ( ) Western blots and quantitative analysis of T-PP2Ac, M-PP2Ac, LCMT1 and PME-1 in the primary neurons. ( ) Western blots for HIF-1 and tau phosphorylation levels at pS396, tau5, M-PP2Ac, T-PP2A and LCMT1 in primary neurons. ( ) Quantification of the relative protein expression levels (HIF-1, pS396, M-PP2Ac and LCMT1) after normalization to the -actin signal. Data represent mean SD, = 3, * < 0.05, ** < 0.01, *** < 0.001 vs. control.",yes
PMC11428392,Figure_5,oa_package/f1/53/PMC11428392.tar.gz,"[' shows examples of the perturbed images used during training.', 'Examples of perturbed images from 3 batches of 8 input images used in training.']",Figure 5 Examples of perturbed images from 3 batches of 8 input images used in training. Each image in a batch has the same perturbation. Bright regions are regions with intensities above 1.0 (due to data augmentation) and are clamped to 1.0 for visualization purposes (stronger contrast between artificial occlusions and bone tissue).,yes
PMC7863388,Figure_8,oa_package/95/dd/PMC7863388.tar.gz,[],Figure 5 Endothelialmacrophage crosstalk regulates CSF1R expression and TJs,yes
PMC8409184,Figure_5,oa_package/44/73/PMC8409184.tar.gz,['Axial computed tomography images.'],Figure 5 A: The patients mother showed multiple thin-walled cysts in the apical segment of the right upper lobe; B: Irregular morphology cysts located in the upper-lung of both sides; C: Bilateral multiple lung cysts located at the level of the upper mediastinum; D: Right-sided pneumothorax and bilateral lung cysts located at both lung bases.,yes
PMC8446518,Figure_2,oa_package/a2/d9/PMC8446518.tar.gz,"[' summarizes probable signaling pathways involved in CM-FB crosstalk regulated by CTGF during IR.', 'Schematic representation of CTGF crosstalk between cardiomyocytes and fibroblasts during cardiac ischemia/reperfusion.']","Figure 2 Schematic representation of CTGF crosstalk between cardiomyocytes and fibroblasts during cardiac ischemia/reperfusion. IR, ischemia/reperfusion; AngI, angiotensin II; TGF-, transforming growth factor beta; CTGF, connective tissue growth factor; ROS, reactive oxygen species; UPR, unfolded protein response; AT1R, angiotensin II receptor type 1; TRI, transforming growth factor beta receptor I; TRII, transforming growth factor beta receptor II; LPR, lipoprotein receptor-related protein/2-macroglobulin receptor; EGFR, epidermal growth factor receptor; M6P/IGF-2R, 6-phosphate/insulin-like growth factor 2 receptor; Int(v3), integrin receptor; CM, cardiomyocytes; CF, cardiac fibroblasts; MyoF, myofibroblasts. The figure was created using .",yes
PMC10435320,Figure_3,oa_package/cc/b7/PMC10435320.tar.gz,[],Figure3 Four common molecular mechanisms underlying the development of irAEs induced by ICIs. (A. disruption of self-immune tolerance; B. cross-reactivity; C. release of cytokines; D. off-target effects).,yes
PMC3464078,Figure_12,oa_package/25/49/PMC3464078.tar.gz,[],"Fig. 12 Septic arthritis and associated osteomyelitis in a diabetic patient. Plain radiograph (A) demonstrates focal soft tissue swelling, demineralization (arrows) in periarticular region in distal interphalangial joint of the first toe. Long-axis T2-weighted fat-suppressed image (B) demonstrated an ulcer and sinus tract (thin white arrow) extending to the joint space. There is a synovial enhancement (arrowhead) and abnormal intramedullary signal (thick white arrow) that is extending from the joint surface in pre-(C) and post-contrast T1-weighted (D) images consistent with septic arthritis and accompanying osteomyelitis.",yes
PMC11128201,Figure_7,oa_package/77/4d/PMC11128201.tar.gz,"['We, thus, examined whether glycolysis is involved in HSPA12A-mediated cardioprotection against MI/R injury (Supplemental A).', 'Cardiac expression of HSPA12A and glycolysis-related genes (GLUT4 and LDHA) decreased 1 hour after reperfusion, and these decreases persisted for at least 24 hours following reperfusion (Supplemental B).', 'Notably, the MI/R-induced downregulation of glucose uptake genes (GLUT1 and GLUT4) and glycolytic enzymes (HK-II and LDHA) was further exaggerated in Hspa12a / hearts, but downregulation of this gene set was alleviated in Hspa12aki hearts relative to the respective WT controls (Supplemental C and Supplemental ).', 'Inhibition of H3 lactylation abolished cardioprotective effects of HSPA12ATo determine the role of H3 lactylation in HSPA12A-mediated cardioprotection, we blocked H3 lactylation using either Oxa to inhibit lactate production or C646 to inhibit p300, which catalyzes binding of lactyl groups to H3 lysine residues (A).', 'Importantly, Oxa treatment also ablated the prosurvival effects of HSP12A in H/R-treated NRCM (C).', 'C646 prevented p300 upregulation in H/R-treated NRCM (D), which was consistent with the previous reports (32).', 'Remarkably, when C646 prevented H3K56la upregulation in H/R-treated Hspa12aO/E NRCM (D), the protective effects of HSP12A on NRCM survival were also abolished (E).', 'Inhibition of H3 lactylation abolished the cardioprotective role of HSPA12A against H/R challenge.']","Figure 7 Inhibition of H3 lactylation abolished the cardioprotective role of HSPA12A against H/R challenge. ( ) Experimental protocol. Two inhibitors, oxamate (Oxa) and C646, were introduced to NRCM culture during reoxygenation. ( ) Lactylation of H3 at lysine 56 (H3K56la) in whole-cell lysates was examined using immunoblotting. The blots for H3 served as loading controls. = 6/group. ( ) NRCM death was examined using PI staining. DAPI was used to counter stain nuclei. = 5/group. ( ) Lactylation of H3 at lysine 56 (H3K56la) and expression of p300 expression in whole-cell lysates were examined using immunoblotting. The blots for H3 served as loading controls. = 5/group. ( ) NRCM death was examined using PI staining. DAPI was used to counter stain nuclei. = 5/group. Data are shown as mean SD. *** 0.001, ** 0.01, and * 0.05 by 2-way ANOVA followed by post hoc test.",yes
PMC6194641,Figure_1,oa_package/be/3b/PMC6194641.tar.gz,"['1).', 'Axial, coronal and three-dimensional reconstruction computed tomography scan with contrast showing a large mass in the right kidney, duplication of the inferior vena cava below the renal veins and compression and displacement of the right inferior vena cava.', 'Straight arrow denotes the right inferior vena cava, and curved arrow the left inferior vena cavaNeither intravascular extension nor invasion to adjacent organs and regional lymph nodes was detected by CT.']","Fig. 1 Axial, coronal and three-dimensional reconstruction computed tomography scan with contrast showing a large mass in the right kidney, duplication of the inferior vena cava below the renal veins and compression and displacement of the right inferior vena cava. Straight arrow denotes the right inferior vena cava, and curved arrow the left inferior vena cava",yes
PMC10591031,Figure_6,oa_package/21/bc/PMC10591031.tar.gz,"['Plasma concentrations of anti-Oprl IgG1 declined significantly after ETI treatment (s 6A and 6B; p = 0.', 'Change in Pseudomonas counts (CFU/mL) were not significant ( 6C; p = 0.', ' 6D demonstrated the regression results for between- and within-subject associations for anti-Oprl IgG1 antibody.', 'A similar strategy was used for the number of hospitalizations, as seen in 6D.', ' 6Change in anti-Oprl serologies following modulator treatment(A) Paired comparison of anti-Oprl IgG1 in plasma acquired from a cohort of adult subjects with CF immediately prior to and on average 5 months post-ETI treatment initiation (n = 20).']","Figure5 Immunization-induced pathology can be transferred through immunized serum (A) Timeline of subcutaneous (s.c.) immunization and infection. C57BL/6J, BALB/c, and mice were immunized with 10 formalin-inactivated (FI) and heat-killed (HK) PA46 three times. Two weeks after the final booster, mice were infected with 10 fresh cultured mucoid PA46 and monitored for 3days (n= 5 each). (B and C) Anti-OprI IgG1 isotype (B)and anti-OprI IgG2c level in C57BL/6J mice or anti-OprI IgG2a level in BALB/c and mice (C). (D) Weight loss at 3days post-infection. Representative H&E staining images are shown in A. (E) Timeline of OprI immunization with different adjuvants to induce strong Th1 immune response in C57BL/6J (n= 5) and BALB/c mice (n= 3). (F and G) Anti-OprI IgG level (F)and anti-OprI IgG2/IgG1 ratio in the immunized group (G), in which IgG2a was measured in BALB/c mice, while IgG2c was measured in C57BL/6J mice. (H) Lung pathological scoring comparison is performed in different mouse strains and adjuvant formulas. Weight loss and representative H&E staining images are shown in B and S3C. (I) Timeline of serum adoptive transfer experiment. C57BL/6J mice were immunized with 10g OprI and 10g LTA1 or 10 FI-PA46 with alum twice, 3weeks apart. Serum was collected through terminal cardiac puncture 1week after final booster. 300L naive or immunized sera was transferred to naive C57BL/6J mice intraperitoneally (i.p.) 2h before challenge. Mice were infected with 10 fresh cultured mucoid PA46 and euthanized 3days after infection (n= 5). (J) Individual anti-OprI IgG level in immunized serum was tested immediately after collection. (K) Anti-OprI IgG2c/IgG1 ratio in pooled immunized sera. (L) Lung pathological scoring in three groups of recipient mice. Weight loss and representative H&E staining images are shown in D and S3E. All statistical comparisons were made using unpaired t test. p<0.05, p<0.0001.",yes
PMC9003981,Figure_3,oa_package/ad/38/PMC9003981.tar.gz,"[' 3A, B; Table 2).', 'The ALFF differences between the radiology interns group (n = 22) and the normal control group (n = 22) (p 0.', 'OFC the orbitofrontal cortex, FG the fusiform gyrusTable 2Peak activations of group ALFF differences between two groups (p 0.', ' 3C, D; Table 3).', ' 3A, B).', ' 3C,D).', ' 3C).']","Fig. 3 The ALFF differences between the radiology interns group ( =22) and the normal control group ( =22) ( <0.05, alphasim corrected, ) and voxel-wise correlation map between ALFF and the level of perceptual expertise in the domain of radiological images as assessed by the AUC of Radiological Expertise Task for the inter RIG group. The intern radiologists group showed higher ALFF in the left OFC (displayed in sagittal view); The intern radiologists group showed higher ALFF in the right fusiform gyrus (displayed in axial view); Significant correlation between ALFF and visual recognition expertise was found the in the right fusiform gyrus ( < ); The scatter plot map computed as ALFF of the peak voxel (40, 56, 16) in the correlation analysis and RET scores. Please note that this map is only for illustration purpose, otherwise there would be the risk of a circular analysis. the orbitofrontal cortex, the fusiform gyrus",yes
PMC9776567,Figure_12,oa_package/a9/c4/PMC9776567.tar.gz,[],"Figure 12 Model interpretability studies using XAI techniques for PSP and AD classification in the entorhinal cortex region of patients with tauopathies. ( ) Guided Grad-CAM analysis (second and fourth column) for AD classification highlights the most prominent NFT of the image; however, for D2 mostly the entire NFT is spotted and for D3 the southwest region of the tangle is spotted. The Occlusion Analysis (third and fifth column) highlights the northern and center region (for D2 and D3) of the image. ( ) Guided Grad-CAM analysis (second and fourth column) for PSP classification mainly highlights all the originally most prominent immunoreactive middle portion of the image (for D2 and D3). Occlusion Analysis (third and fifth column) highlights the center of the image near the apex of the central NFT (for D2 and D3). In the green channel ( , ), immunoreactivity of the AT8 antibody directed against the biomarker for dual phosphorylation at serine 202 and threonine 305 of the Tau polypeptide is observed ( , ). The red channel ( , ) shows staining with Thiazine red dye for fibrillar forms of pathological Tau aggregates. The blue channel ( , ) shows immunoreactivity of the pS396 antibody directed against the biomarker for phosphorylation at amino acid serine 396 of Tau protein.",yes
PMC11543643,Figure_5,oa_package/e2/2b/PMC11543643.tar.gz,"['Finally, the plate was fixed distally with one cortical and three locking screws and fixed proximally with 5 variable angle monocortical locking screws directed anteriorly and posteriorly, avoiding the femoral stem (', ' 5(a) Postoperative anteroposterior and (b) oblique proximal femur radiographs show fixation, as described.']","Figure5 (a) Postoperative anteroposterior and (b) oblique proximal femur radiographs show fixation, as described. Note the unicortical screws proximally.",yes
PMC8393371,Figure_2,oa_package/de/b8/PMC8393371.tar.gz,"['The human vascular smooth muscle cells-expressed NOX isoforms, NOX1, NOX2, NOX4, and NOX5 isoforms, require an isoform-specific assembly of different subunits or a substance for the activation () [19,20,21].', 'NOX 1, 2, 4, and 5 are expressed in human vascular smooth muscle cells.']","Figure 2 NOX 1, 2, 4, and 5 are expressed in human vascular smooth muscle cells. NOX1, 2, and 4 have the transmembrane subunit p22phox both in the active and inactive forms. NOX1 interacts with the cytosolic subunits NOXO1 or p47phox, NOXA1 or p67phox, and rac1 upon activation. NOX2 activates when it is associated with p47phox, p67phox, p40phox, and rac1. NOX4 is constitutively activated with the cytosolic p22phox subunit. NOX5 needs Ca binding in the cytosol for activation.",yes
PMC11019475,Figure_4,oa_package/45/5e/PMC11019475.tar.gz,['A non-contrast head CT angiogram demonstrating an STA-MCA bypass.'],"Figure 4 A non-contrast head CT angiogram demonstrating an STA-MCA bypass. Panels A and B depict computerized reconstruction. Panels C and D highlight an area of decreased blood vessel density due to artifact (blue circles). STA: superficial temporal artery, MCA: middle cerebral artery",yes
PMC2731738,Figure_1,oa_package/3e/37/PMC2731738.tar.gz,['Primary cultures of trigeminal satellite cells.'],Figure 1 . Morphology of a primary culture of rat satellite cells was observed under phase-contrast microscopy. The predominant phenotype consists of small and dark cells with an elongated shape. (10 50 m scale bar),yes
PMC3670535,Figure_2,oa_package/eb/d3/PMC3670535.tar.gz,"['The final histopathological examination sets the diagnosis of mucinous appendiceal cystadenoma (), endometrial adenocarcinoma (type I, grade I), (), and benign cysts of both ovaries.', '001""/>Mucinous cystadenoma of the appendix.']",Figure 2 Mucinous cystadenoma of the appendix. Hematoxylin eosin stain.,yes
PMC8623118,Figure_1,oa_package/43/8e/PMC8623118.tar.gz,"['An example of the variance of size measurements among different MRI pulse sequences is shown in .', '0b013e3182372c4022082543HCC size measurements vary between different MRI pulse sequences.', ' shows an HCC in the right liver lobe measuring 4.']","Figure 1 HCC size measurements vary between different MRI pulse sequences. shows an HCC in the right liver lobe measuring 4.9 cm in the T2w half-Fourier-acquired single-shot turbo spin echo sequence ( ), 5.2 cm in the T2w turbo spin-echo sequence ( ), 4.9 cm in the T1w volumetric interpolated breath-hold examination sequence ( ) pre-contrast ( ), 5.3 cm in the arterial phase ( ), 5.2 cm in the venous phase ( ), and 4.8 cm in the hepatobiliary phase ( ). Histopathologic assessment revealed a tumor diameter of 4.5 cm.",yes
PMC8387825,Figure_1,oa_package/f1/0c/PMC8387825.tar.gz,"['1"">Case 1A 75-year-old man with a past medical history of PV isolated to the nose, hypertension, hyperlipidemia, and osteoporosis presented with a 4-week history of erosions on the bilateral nasal alae ().', 'Case 1: Pemphigus vulgaris.']",Fig 1 Case 1: Pemphigus vulgaris. Crusted erosion on the right side of the nose.,yes
PMC3840745,Figure_1,oa_package/d1/67/PMC3840745.tar.gz,"['LPL was found in all major cortical areas (frontal, parietal, temporal and occipital lobes) (A-D).', 'LPL immunoreactivity was present in leptomeninges, where it associated with smooth muscle cells, endothelial cells, some arachnoid cells and microglia/macrophages (E).', '.', '1369_0022155413505601-fig1""/>White matter areas throughout the brain featured extensive microglial staining, both cytoplasmic and nuclear, with more restrictive immunoreactivity in astrocytes and oligodendroglia.', 'In the hippocampus (F-H), LPL immunoreactivity was strongly positive in the ependymal cells, predominantly in the apical area.', 'The dentate gyrus displayed robust neuronal and the synaptic network staining (G), and neurons in the Cornu Ammonis (CA) layers 1, 2, 3 and 4 were also positive, with no significant differences in the intensity of staining between different CA neuronal groups.', 'The parahippocampal cortex had a similar immunostaining pattern and the choroid plexus epithelium showed high LPL immunoreactivity (I).', 'LPL immunostaining was also strong in the striatal neurons, whereas thalamic neurons showed moderate to strong staining (J).', 'LPL immunoreactivity was also found in the third cranial nerve nucleus (K).', 'Pontine nuclei showed positive LPL immunoreactivity, and some positive staining was visible in the Schwann cells associated with cranial nerves (L).', 'Strong LPL staining was present in the medium to large neurons in the medulla (M), including the inferior olivary nuclei.', 'Neurons in the spinal cord were weakly stained in the anterior horn, and some scattered neuronal staining in other areas could be observed (N).', 'Cerebellar dentate neurons were strongly immuno-positive for LPL, whereas granular neurons and molecular layer showed weakly positive staining (O-P).', 'The pituitary gland was strongly positive for LPL in both the anterior and posterior pituitary (Q-R).']","Figure 1. LPL in normal human brain. (A) Frontal cortex. (B) Parietal cortex. (C) Temporal cortex. (D) Occipital cortex. (E) Leptomeningae. Long black arrow, smooth muscle cells; short black arrow, endothelial cell; blue arrow, subpial microglia; red arrow, arachnoid. (F) Low magnification of hippocampus. (G) Dentate gyrus. (H) Hippocampal neurons. (I) Choroid plexus. (J) Thalamus. (K) Midbrain, third cranial nerve nucleus. (L) Cranial nerves with Schwann cells. (M) Medulla. (N) Spinal cord. (O) Cerebellum. (P) Cerebellum, dentate neurons. (Q) Pituitary, anterior (right) and posterior (left). (R) Anterior pituitary, detail. Scale bars = 50 m (A-E, G-P and R) and 100 m (E and Q).",yes
PMC9313434,Figure_5,oa_package/e1/c9/PMC9313434.tar.gz,"['vivoPMO-PACS4 Treatment Significantly Reduces Muscle Turnover and Muscle FibrosisSince muscle regeneration in FSHD is correlated with DUX4 pathological severity [43], we further quantified the number of centrally nucleated fibers (CNFs) in both the TA and DIA muscles (a,d,g).', 'We therefore additionally examined the level of fibrosis in TA and DIA muscles using immunostaining for a commonly used marker of fibrosis, collagen VI [45,46] (b,e,g).', '0026) as compared to the levels seen in the SCR-treated TA and DIA, respectively (c,f).', 'vivoPMO-PACS4 treatment reduces muscle deterioration and muscle fibrosis.']","Figure 5 vivoPMO-PACS4 treatment reduces muscle deterioration and muscle fibrosis. The tibialis anterior (TA) and diaphragm (DIA) muscle sections were stained for laminin or collagen VI; DAPI was used for nuclear staining. The total number of centrally nucleated myofibers (CNFs) of ( ) TA and ( ) DIA muscles was scored automatically and is expressed as a percentage of the total myofiber number within the same transverse muscle section. Fibrotic area in ( ) TA and ( ) DIA muscles was semi-automatically evaluated and expressed as percentage of the area positive for collagen VI of the muscle CSA. mRNA expression of indicative for fibrotic response in ( ) TA and ( ) DIA muscles was quantified by qRT-PCR as relative to and shown as a fold-change of SCR values obtained by the same way. Data are shown as means SEM; = 6. Statistical comparison was by one-way ANOVA followed by Tukeys multiple comparisons test; * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001. ( ) Representative images of the entire muscle cross-sections analyzed by Fiji/MuscleJ for CNFs, with fibers having 0, 1, 2 and 3+ CNFs color-coded as white, yellow, orange and red, respectively. Images of transverse muscle sections immunostained with collagen VI are shown at a magnification of 100, scale bar = 500 m. Corresponding enlarged images at higher magnification are shown in the subsets.",yes
PMC8818517,Figure_5,oa_package/81/4c/PMC8818517.tar.gz,"['A partial-thickness skin graft was taken from the right thigh area, and tie-over beads were placed and fixed with a sterile bandage [].', 'An excisional biopsy was also performed and sent to the pathology lab (Size11 9.']",Fig. 5 Fixation of tie-over beads with sterile bandages.,yes
PMC5067335,Figure_4,oa_package/b7/4a/PMC5067335.tar.gz,"['003""/>CCL25 expression in the lungs of allergen sensitized mice.']","Figure 4 CCL25 expression in the lungs of allergen sensitized mice. (a) Mice were sacrificed 6, 24, and 48h, respectively, after the last OVA-challenge. (b) CCR9 and CCL25 expression in lungs were quantified by real-time RT-PCR. (c) CCL25 expression in lungs of CCR9 KO and WT mice. Data are representative of 3 independent experiments and represent mean SD. = 2-3 for each group ( < 0.05 when compared with WT mice or when compared each time with control group).",yes
PMC10866854,Figure_14,oa_package/36/fb/PMC10866854.tar.gz,[],"Fig. 14 Example of left ovarian lesion with a solid component with a type I dynamic enhancement curve. Axial T2-weighted sequence without fat saturation. Axial DWI scan ( : 1200 s/mm ). Axial unenhanced T1-weighted water sequence. Axial late-gadolinium T1-weighted sequence. , DCE MRI T1-weighted sequence with corresponding curve of the tissular component showing a type I curve. This was favored to be a benign cystadenofibroma",yes
PMC9495225,Figure_1,oa_package/53/3a/PMC9495225.tar.gz,"['Mitral regurgitation appeared central in 24 dogs and eccentric in the remaining 2 dogs, of which in one case two small jets were noticed ().', 'On the other hand, though presumed physiologic mitral valve regurgitations are thought to cause a central jet [5,18], we did identify dogs with an eccentric and more than one jet (C).', '02019272821Color Doppler echocardiographic images (systolic frames) of trivial mitral valve regurgitation jets in three clinically healthy Labrador retrievers without heart murmur.']",Figure 1 Color Doppler echocardiographic images (systolic frames) of trivial mitral valve regurgitation jets in three clinically healthy Labrador retrievers without heart murmur. ( ). Standard right parasternal four-chamber view showing a central jet. ( ). Standard left parasternal four-chamber view showing a central jet. ( ). Standard right parasternal four-chamber view showing two eccentric jets.,yes
PMC4055699,Figure_2,oa_package/92/63/PMC4055699.tar.gz,"['Mass spectrometry analysis on these cells ( A) identified over 200 proteins, which were prioritised by fold enrichment using spectral counting.', 'g002REIIBP interacts with the SMN complex in myeloma t(4;14) cells.', 'In order to validate the REIIBP/SMN interaction, we carried out western blotting on H929 and H929::REIIBP following anti-FLAG pull-down ( B) using antibodies against different members of the SMN complex.', 'GEMIN5 co-immuno-precipitation (Co-IP) pulled down GEMIN3, a member of the SMN complex ( C).', 'Co-IP of the SMN protein showed a similar result ( D), confirming that endogenous REIIBP is bound to the whole SMN complex, and not only to certain members.']",10.1371/journal.pone.0099493.g002,yes
PMC11538668,Figure_1,oa_package/4d/18/PMC11538668.tar.gz,"['Spatial transcriptomic profiling of the murine livers in fed, fasted and refed conditions.']","Figure 1 Spatial transcriptomic profiling of the murine livers in fed, fasted and refed conditions. A) Left top: H&E staining of liver sections from ctrl, fasted and refed mice. Right: UMAP visualization of 12 identified clusters. Left bottom: Spatial distribution of each cluster in liver tissue sections captured on 10x Visium slides. B) Pseudotime inference by the DPT algorithm. C) Heatmap displaying mRNA expression of selected genes detected by ST from periportal (PN) to centrilobular (CV) regions. D) Projection of selected marker genes in UMAP space. E) Left, Regression analysis defines five zonation patterns, displaying the standard fit model, top five genes by zonation index, and expression values for a representative gene across pseudotime for each pattern. Right, Sankey diagram depicting zonation pattern transitions across nutritional states. F) Gene expression changes along the PNCV axis. Orange, red, and green represent ctrl, fasted, and refed samples. Ribbons within the fitted line represent standard error of gene expression.",yes
PMC7734537,Figure_2,oa_package/b5/52/PMC7734537.tar.gz,"['Tumor cells grow in sheets or nests separated into lobules by fibrous septa containing small lymphocytes ().', '.', '1177_2374289520975173-fig2""/>Embryonal carcinomaEmbryonal carcinoma is the second most common pure testicular germ cell neoplasm after seminoma.']","Figure 2. Histologic features of seminoma. This photomicrograph shows nests of uniform seminomatous cells with abundant clear cytoplasm, centrally located nuclei, and distinct cells membranes. Fibrovascular septa with small lymphocytes are characteristic of seminoma (200).",yes
PMC5996913,Figure_4,oa_package/0f/63/PMC5996913.tar.gz,"['Serum levels of fT3\n(A), fT4\n(B), and TSH (C) in vitiligo patients compared with control subjects.']","Figure 4 Serum levels of fT , fT , and TSH in vitiligo patients compared with control subjects. Values expressed as meanSEM. *** <0.001 significant vs. Control. <0.001 significant vs. SVP, b; non-significant vs. SVP (between groups Total vs. Total; Male vs. Male; Female vs. Female). NS is a non-significant within group (Male vs. Female). Abbreviations: C, control; SVP, stable vitiligo patient; AVP, active vitiligo patient; M, male; F, female; T, total; fT , free triiodothyronine; fT , free thyroxine; TSH, thyroid-stimulating hormone.",yes
PMC8571205,Figure_2,oa_package/71/2b/PMC8571205.tar.gz,[':3D reconstruction of the lumbosacral spine showing the l5 wide transverse process with a bony ridge connecting the l5 to the sacrum.'],Figure 2: 3D reconstruction of the lumbosacral spine showing the l5 wide transverse process with a bony ridge connecting the l5 to the sacrum. (Red circle).,yes
PMC10784825,Figure_1,oa_package/30/ec/PMC10784825.tar.gz,['\nIntraoperative Cholangiogram.'],Figure 1 The preferred intraoperative imaging is cholangiogram. A: Laparscopic view showing a catheter (short arrow) for the dye injection passing through the bile duct (long arrow); B: Opacified bile ducts. Note the clips at the cystic duct stump (arrow).,yes
PMC10627867,Figure_1,oa_package/80/98/PMC10627867.tar.gz,"['A 6- to 8-cm incision is marked, centered over the medial epicondyle ().', 'Right medial elbow.']",Fig 1 Right medial elbow. The patient is positioned supine and a hand table attachment is used. A 6-cm incision is marked over the medial epicondyle. The ulnar nerve is palpated and marked.,yes
PMC10450110,Figure_6,oa_package/45/95/PMC10450110.tar.gz,"['In patients with the frank purulent collection and with thick subcutaneous fat layer, an additional corrugated rubber drain was also placed in this layer [].', 'Skin closed with corrugated rubber drain in the subcutaneous plane in cases with frankly purulent intra-abdominal collection as well as cases with thick subcutaneous fatFor the patient who required ileostomy, a loop ileostomy was fashioned on the lateral aspect of the same subumbilical right transverse incision, keeping the proximal limb towards the lateral side and the distal limb towards the medial side [].']",Figure 6 Skin closed with corrugated rubber drain in the subcutaneous plane in cases with frankly purulent intra-abdominal collection as well as cases with thick subcutaneous fat,yes
PMC11602531,Figure_4,oa_package/7c/9d/PMC11602531.tar.gz,"['Expanding hematoma, 34 mm in depth from the sternumDuring these events, the patient remained hemodynamically stable.']","Figure 4 Expanding hematoma, 34 mm in depth from the sternum",yes
PMC10529449,Figure_4,oa_package/08/97/PMC10529449.tar.gz,"['() where the SC were stratified according to whether the posterior wall was left in place or not and whether the remaining part of the gallbladder was closed or not [22].', 'Laparoscopic subtotal cholecystectomy (LSC) classification scheme.']","Figure 4 Laparoscopic subtotal cholecystectomy (LSC) classification scheme. Type 1A: normal laparoscopic cholecystectomy. Type 1B: the back wall of the gallbladder (GB) is fully dissected off the liver bed and Hartmanns pouch is closed following evacuation of gallstones. Type 1C: the back wall of the GB is left in situ and Hartmanns pouch is closed. Type 2A: the back wall of the GB is excised but Hartmanns pouch is left open. Type 2B LSC: the back wall of the GB is left in situ and Hartmanns pouch is left open. Type 2C LSC: the fundus of the GB is deroofed, the stones are evacuated and the GB remnant is left open. These illustrations were created by the Toronto Video Atlas of Surgery ( (accessed on 5 September 2023)) [ ].",yes
PMC7938226,Figure_14,oa_package/c8/76/PMC7938226.tar.gz,[],,yes
PMC3789892,Figure_2,oa_package/b0/56/PMC3789892.tar.gz,"[' 2a), i.', ' 2b), mitral cell layer (Mit, ', ' 2c), and in granule cell layer (GCL, ', ' 2d).', ' 2a d).', ' 2a d; supplementary Table 2).', ' 2e).', '', 'Scatter plots show data from individual mice\nWe detected t -syn both by its ATTO-550 fluorescent tag (in red), and by hu -syn (syn211) immunostaining (in blue) using confocal microscopy (', ' 2e).', ' 2a d, 3d).', 'pdf"">Supplementary (PDF 786 kb)\n(a e) Histopathological and immunohistochemical findings.']","Fig. 2 (ae) Histopathological and immunohistochemical findings. Histopathology of the tumor in the bladder trigone revealed benign prostatic tissue and prostatic adenocarcinoma with a dominant Gleason Grade 4 pattern. (a) Hematoxylin and eosin staining, 40 magnification. (b) Positive immunostaining of PSA, 40 magnification. (c) Positive immunostaining of p40, which is a basal cell marker for benign prostatic glands, 40 magnification. (d) Histopathological findings indicating normal urothelial mucosa and adenocarcinomainfiltrated submucosa. Hematoxylin and eosin staining, 10 magnification. (e) Pathological examination of a transrectal prostate needle biopsy reveals prostate adenocarcinoma, Gleason Grade group 1, on hematoxylin and eosin staining, 10 magnification.",yes
PMC10332756,Figure_1,oa_package/5a/9d/PMC10332756.tar.gz,[],,yes
PMC6711701,Figure_1,oa_package/79/f5/PMC6711701.tar.gz,"['Cerebral MRI () was performed showing a first right cerebellar convexity lesion which was hyper intense on T2 weighted images containing small zones of necrosis in hyper intense T2 weighted signal and discrete hyper intense T1.', 'Cerebral MRI: two cerebellar lesions: iso signal weighted T1, hyper signal weighted T2, seat of small area of necrosis, heterogeneously enhanced, with hyper signal diffusion without decrease of the ADC and surrounded by oedemaDiscussionThe Lhermitte-Duclos disease, or dysplastic cerebellar gangliocytoma, is an unusual tumour arising from the cerebellar cortex.']","Figure 1 Cerebral MRI: two cerebellar lesions: iso signal weighted T1, hyper signal weighted T2, seat of small area of necrosis, heterogeneously enhanced, with hyper signal diffusion without decrease of the ADC and surrounded by oedema",yes
PMC8983543,Figure_7,oa_package/03/6f/PMC8983543.tar.gz,"[' 7).', 'Solid papillary carcinoma characterized by a low-grade atypical epithelial proliferation with solid growth pattern filling dilated ducts (A).', 'Neuroendocrine differentiation is frequently found as confirmed by expression of synaptophysin (C)Molecular studies included only a limited number of cases.']",Fig. 7 Solid papillary carcinoma characterized by a low-grade atypical epithelial proliferation with solid growth pattern filling dilated ducts ( ). Invasion is characterized by small infiltrative ducts and nests ( ). Neuroendocrine differentiation is frequently found as confirmed by expression of synaptophysin ( ),yes
PMC3205758,Figure_14,oa_package/a4/f8/PMC3205758.tar.gz,[],Figure 14 Pelvic peritoneal involvement. (a) Sagittal T2-weighted MRI depicting peritoneal thickening and nodularity (arrows). Sagittal T1-weighted fat-saturated MRI (b) before and (c) after intravenous injection of gadolinium demonstrating marked abnormal peritoneal enhancement. Ascitic fluid (F) outlines the pelvic peritoneal spaces.,yes
PMC4396520,Figure_3,oa_package/fb/5f/PMC4396520.tar.gz,"['The CS group showed significantly higher levels of both protein expression levels (Western blot, A, B; immunohistochemistry, E), and MMP2 and MMP9 activities (C, D), than the other two groups.', 'The protein expression levels and activities of MMP2 and MMP9 in the RCS group were all higher than in the control group ().', 'Effects of rosiglitazone on the expression of the NF B p65, I B , and pI B by Western blotting (A).']","Figure 3 Effects of rosiglitazone on the expression of MMP2 and MMP9 in Cs-induced emphysema in rats. Effects of rosiglitazone on the expression of MMP2 and MMP9 in CS-induced emphysema in rats as measured by Western blotting ( ). The target protein bands were desitometrically analyzed normalized to -actin ( ). The activities of MMP2 ( ) and MMP9 ( ) in each group were determined by using MMP2 and MMP9 Biotrak activity assay. The expression of MMP2 and MMP9 was confirmed by using immunohistochemistry for each group (magnification 400) ( ). The values presented are the mean SD. * <0.05, compared with the control group; <0.05, compared with the CS group. MMP, metalloprotease; CS, cigarette smoke; SD, standard deviation; RCS, rosiglitazone-cigarette smoke.",yes
PMC6636106,Figure_2,oa_package/4a/17/PMC6636106.tar.gz,"[' 2) in 7/38 cases.', '2Serous cystic neoplasm with irregular shapeSerous cystic neoplasm with thin wall (1.']",Fig. 2 Serous cystic neoplasm with irregular shape,yes
PMC6803513,Figure_3,oa_package/12/7e/PMC6803513.tar.gz,"['8% of voxels) at the 90% threshold for significance (), indicating that greater intraindividual variability across neuropsychological test performance corresponded with decreased microstructural in these areas.', 'Positive associations between intraindividual standard deviation (ISD)-BP and white matter, based on mean diffusivity (MD) at 90% threshold, depicted in radiological view.']","Figure 3 Positive associations between intraindividual standard deviation (ISD)-BP and white matter, based on mean diffusivity (MD) at 90% threshold, depicted in radiological view. Mean FA skeleton shown in green. Areas of significant association shown in red.",yes
PMC10415516,Figure_3,oa_package/dc/d7/PMC10415516.tar.gz,"[' 3).', 'The acetabular index (AI) is the angle formed by Hilgenreiner line (Line 1) and a line drawn from the triradiate epiphysis to the lateral edge of the acetabulum (Line 2)A senior pediatric orthopedic surgeon measured all PFD values in this study.']",Fig. 3 The acetabular index (AI) is the angle formed by Hilgenreiner line (Line 1) and a line drawn from the triradiate epiphysis to the lateral edge of the acetabulum (Line 2),yes
PMC7430590,Figure_1,oa_package/93/cb/PMC7430590.tar.gz,"['RESULTSAntibody repertoire analysis of COVID-19 patientsWe first characterized the B cell response in COVID-19 using single-cell Ig gene sequencing of human mAbs (A).', '1%) within S1-MBC (B and 1C).', 'Binding to S1 did not depend on affinity maturation as measured by the number of SHM (D).', 'Of 86 mAbs strongly binding to RBD, 40 showed virus neutralization with a half-maximal inhibitory concentration (IC50) 250 ng/ml and were considered neutralizing antibodies (A, Supplementary Table ST2), from which 18 (Top-18) were selected for further characterization (Supplementary Table ST4).', '2 ng/ml (E) and an equilibrium dissociation constant (KD) of 6.', '1 172 ng/ml (F, Supplementary Table ST4).', 'The antibody with the highest affinity, CV07 209, was also the strongest neutralizer (G).', 'Near-germline SARS-CoV-2 neutralizing antibodies can bind to murine tissueMany SARS-CoV-2 neutralizing mAbs carry few SHM or are in germline-configuration (D) (Ju et al.', 'Assays for concentration-dependent RBD binding (E) were developed using 1-step Slow TMB (Thermo Fisher Scientific).', ' |Identification and characterization of potent SARS-CoV-2 neutralizing mAbs.']","Fig. 1 | Identification and characterization of potent SARS-CoV-2 neutralizing mAbs. ( ) Diagram depicting the strategy for isolation of 18 potently neutralizing mAbs (Top-18). ( ) Normalized binding to S1 of SARS-CoV-2 for mAbs isolated from antibody secreting cells (; blue = S1-binding, grey = not S1-binding). (OD=optical density in ELISA) ( ) Normalized binding to S1 of SARS-CoV-2 for mAbs isolated from S1-stained memory B cells (; colors like in (B)) ( ) S1-binding plotted against the number of somatic hypermutations (SHM) for all S1-reactive mAbs. ( ) Concentration-dependent binding of Top-18 SARS-CoV-2 mAbs to the RBD of S1 (meanSD from two wells of one experiment). ( ) Concentration-dependent neutralization of authentic SARS-CoV-2 plaque formation by Top-18 mAbs (meanSD from two independent measurements). ( ) Affinity of mAbs to RBDs (K determined by surface plasmon resonance) plotted against IC of authentic SARS-CoV-2 neutralization.",yes
PMC10672373,Figure_14,oa_package/4c/14/PMC10672373.tar.gz,[],"Figure 14 A 50-year-old male patient referred to the ICU after major trauma. Bedside CXR ( ) and LUS ( ). ( ) The CXR showed the surgical drainage tube (arrowhead), with poor confidence on the entity of the residual pneumothorax. ( ) LUS showed the presence of lungs points (arrow) confirming the residual pneumothorax that was not clearly demonstrable on the CXR.",yes
PMC3270029,Figure_7,oa_package/49/c4/PMC3270029.tar.gz,"['7B, D, F), but not the young NT mice (A), and it was related to tauopathy development rather than aging as parallel-processed, old littermate control mice were negative (Figs.', 'The distribution of histopathology in the old NT mouse was extensive, with robust staining being seen in cells in the EC, as well as in the subiculum (D).', 'Staining was also extensive in the CA1, but to a lesser extent in the DG GC layer (F).', 'g007Silver staining in NT mice.', '\nA shows a lower power image of argyrophilic material in the hippocampus and cortex of a young NT mouse (', '7A), an old NT mouse (B) and a control mouse (age matched to the old NT mouse) (', 'Higher power images from the EC and subiculum of the old NT mouse (D) or control mouse (', '7F and G show high power images of argyrophilic neurons in the hippocampus of the old NT mouse (F) or control mouse (']",10.1371/journal.pone.0031302.g007,yes
PMC11558486,Figure_7,oa_package/88/21/PMC11558486.tar.gz,"['Fractures involving other bony structures of the chest wall (clavicle, scapula, sternum, and vertebral spinous processes) exhibit similar findings to those of ribs ().', 'Clavicular fracture.']","Figure 7 Clavicular fracture. A 75-year-old male with shoulder pain and hematoma following a fall two weeks ago. (A) Panoramic view of the clavicle shows a proximal cortical disruption (arrow) adjacent to the sternoclavicular joint (arrowhead), consistent with clavicular fracture. (B) Chest CT scan done posteriorly to accurately characterize the fracture shows amultifragmentary proximal clavicular fracture. It is important to note the limitations of ultrasound in evaluating the posterior cortex and, generally, the features of bone fractures. CT, computed tomography.",yes
PMC9309514,Figure_1,oa_package/50/65/PMC9309514.tar.gz,"['Whole-body CT showed enlargement of the left testicle and retroperitoneal lymph nodes (A).', 'Imaging and magnetic resonance spectroscopy findings.', 'MR spectroscopy revealed an increased choline peak and an abnormal lactate peak, but the N-acetyl aspartate peak was preserved (G).']","Figure 1 Imaging and magnetic resonance spectroscopy findings. Body trunk computed tomography showed enlargement of the left testicle with heterogeneous content (arrow) and retroperitoneal lymph node (arrowhead). Brain magnetic resonance imaging (MRI) on admission showed a high-intensity lesion in diffusion-weighted and fluid-attenuated inversion recovery (FLAIR) images, with iso-intensity in apparent diffusion coherence images. The brain lesion enlarged afterward, but with no gadolinium enhancement. Magnetic resonance spectroscopy showed high choline (Cho) peak, preserved N-acetyl aspartate (NAA) peak, and lactate (Lac) peak. Cr, creatine.",yes
PMC10154360,Figure_1,oa_package/7b/e6/PMC10154360.tar.gz,[],"FIGURE 1 Paraffin sections of formalin fixed paraffin embedded (FFPE) brains of wild type (WT) (top) and D1113H (bottom) 8weekold mice. (A,D) H&E stain of cortex demonstrated no developmental abnormalities or histopathology. Specifically, there was no evidence of immune cell infiltration. Immunohistochemical stain for GFAP (B,E) and IBA1 (C,F) showed no evidence of significant astrocytosis or microgliosis. Bar=200 microns",yes
PMC4102011,Figure_1,oa_package/37/04/PMC4102011.tar.gz,"['At Doppler US, the right CCA diameter was found to be 8 mm, whereas the left one came out to be 4 mm (s 1(a) and 1(b)).', 'In our case, the right CCA diameter was 8 mm, while the left one was found to be only 4 mm, thus pointing to its hypoplastic situation (s 1(a) and 1(b)).', '0-003407777210647740(a) Doppler US images demonstrate the diameters of the right and left CCA, which are 8 mm and 4 mm, in respect.']","Figure 1 (a) Doppler US images demonstrate the diameters of the right and left CCA, which are 8mm and4 mm, in respect. (b) These axial Doppler US images reveal the diameters of the right and left ICA. (c) The Doppler view of the left ECA and its branching. (d) The spectral drawing demonstrating that the vessel mentioned in is indeed the ECA.",yes
PMC9743601,Figure_3,oa_package/f8/fe/PMC9743601.tar.gz,"[' 3).', ' 3).', 'Coronal brain sections of three representative individuals per group euthanized at the end of the microdialysis experiment, i.', '6 h or 24 h after the intravenous injection of [125I]I-mAb3D6, [125I]I-mAb3D6-scFv8D3 or 125IGlial cell markers Iba1 and GFAP around the probe areaProteins Iba1 (microglia) and GFAP (astrocytes) were studied around the probe area in the brain by immunohistochemistry to detect potential immune responses caused by the guide cannula surgery and probe insertion, and to reveal effects related to immunotherapy, which could explain the difference in the brain distribution of the two antibodies.']","Fig. 3 Coronal brain sections of three representative individuals per group euthanized at the end of the microdialysis experiment, i.e. 6h or 24h after the intravenous injection of [ I]I-mAb3D6, [ I]I-mAb3D6-scFv8D3 or I",yes
PMC6531305,Figure_1,oa_package/50/c2/PMC6531305.tar.gz,"['Primary hypothyroidism is associated with a reduced incidence of primary breast carcinomaCancer200510311228\n1571237512HercbergsAAGoyalLKSuhJHLeeSReddyCACohenBHPropylthiouracil-induced chemical hypothyroidism with high-dose tamoxifen prolongs survival in recurrent high grade glioma: a phase I/II studyAnticancer Res20032361726\n1268015713NessRBGrissoJACottreauCKlapperJVergonaRWheelerJEFactors related to inflammation of the ovarian epithelium and risk of ovarian cancerEpidemiology2000111117\n1102160614ChiHCChenSLLiaoCJLiaoCHTsaiMMLinYHThyroid hormone receptors promote metastasis of human hepatoma cells via regulation of TRAILCell Death Differ201219180214\n2257666215ChungIHChenCYLinYHChiHCHuangYHTaiPJThyroid hormone-mediated regulation of lipocalin 2 through the Met/FAK pathway in liver cancerOncotarget201561505064\n2594079716LinYHLiaoCJHuangYHWuMHChiHCWuSMThyroid hormone receptor represses miR-17 expression to enhance tumor metastasis in human hepatoma cellsOncogene201332450918\n2391245217WuSMHuangYHYehCTTsaiMMLiaoCHChengWLCathepsin H regulated by the thyroid hormone receptors associate with tumor invasion in human hepatoma cellsOncogene201130205769\n2121777618EncinarJAMalloGVMizyryckiCGionoLGonzalez-RosJMRicoMHuman p8 is a HMG-I/Y-like protein with DNA binding activity enhanced by phosphorylationJ Biol Chem2001276274251\n1105616919MalloGVFiedlerFCalvoELOrtizEMVasseurSKeimVCloning and expression of the rat p8 cDNA, a new gene activated in pancreas during the acute phase of pancreatitis, pancreatic development, and regeneration, and which promotes cellular growthJ Biol Chem1997272323609\n940544420SandiMJHamidiTMalicetCCanoCLoncleCPierresAp8 expression controls pancreatic cancer cell migration, invasion, adhesion, and tumorigenesisJ Cell Physiol2011226344251\n2134439721EmmaMRIovannaJLBachvarovDPuleioRLoriaGRAugelloGNUPR1, a new target in liver cancer: implication in controlling cell growth, migration, invasion and sorafenib resistanceCell Death Dis20167e2269\n2733671322LeeYKJeeBAKwonSMYoonYSXuWGWangHJIdentification of a mitochondrial defect gene signature reveals NUPR1 as a key regulator of liver cancer progressionHepatology201562117489\n2617306823LinKHShiehHYHsuHCNegative regulation of the antimetastatic gene Nm23-H1 by thyroid hormone receptorsEndocrinology200014125407\n1087525624ShihCHChenSLYenCCHuangYHChenCDLeeYSThyroid hormone receptor-dependent transcriptional regulation of fibrinogen and coagulation proteinsEndocrinology2004145280414\n1497786025LinYHWuMHLiaoCJHuangYHChiHCWuSMRepression of microRNA-130b by thyroid hormone enhances cell motilityJ Hepatol201562132840\n2561749526LiuXZhengNShiYNYuanJLiLThyroid hormone induced angiogenesis through the integrin alphavbeta3/protein kinase D/histone deacetylase 5 signaling pathwayJ Mol Endocrinol20145224554\n2453265627MousaSALinHYTangHYHercbergsALuidensMKDavisPJModulation of angiogenesis by thyroid hormone and hormone analogues: implications for cancer managementAngiogenesis2014174639\n2445869328PintoMSoaresPRibattiDThyroid hormone as a regulator of tumor induced angiogenesisCancer Lett201130111926\n2118327529LiuDZhangYWeiYLiuGLiuYGaoQActivation of AKT pathway by Nrf2/PDGFA feedback loop contributes to HCC progressionOncotarget2016765389402\n2758848330TanikawaAAGrottoRMSilvaGFFerrasiACSarnighausenVCPardiniMIPlatelet-derived growth factor A mRNA in platelets is associated with the degree of hepatic fibrosis in chronic hepatitis CRev Soc Bras Med Trop2017501136\n2832781231ZiogasIATsoulfasGEvolving role of Sorafenib in the management of hepatocellular carcinomaWorld J Clin Oncol2017820313\n2863879032ZhuYJZhengBWangHYChenLNew knowledge of the mechanisms of sorafenib resistance in liver cancerActa Pharmacol Sin20173861422\n2834432333ChiHCChenSLChengYHLinTKTsaiCYTsaiMMChemotherapy resistance and metastasis-promoting effects of thyroid hormone in hepatocarcinoma cells are mediated by suppression of FoxO1 and Bim pathwayCell Death Dis20167e2324\n2749092934ChuYDLinKHHuangYHLinCCHungCFYehTSA novel thyroid function index associated with opposite therapeutic outcomes in advanced hepatocellular carcinoma patients receiving chemotherapy or sorafenib\nAsia Pac J Clin Oncol2018\n35FernandezMSemelaDBruixJColleIPinzaniMBoschJAngiogenesis in liver diseaseJ Hepatol20095060420\n1915762536KimWGChengSYThyroid hormone receptors and cancerBiochim Biophys Acta20131830392836\n2250726937RennertGRennertHSPinchevMGruberSBA case-control study of levothyroxine and the risk of colorectal cancerJ Natl Cancer Inst201010256872\n2030512938HassanMMKasebALiDPattYZVautheyJNThomasMBAssociation between hypothyroidism and hepatocellular carcinoma: a case-control study in the United StatesHepatology200949156370\n1939991139ChenRNHuangYHYehCTLiaoCHLinKHThyroid hormone receptors suppress pituitary tumor transforming gene 1 activity in hepatomaCancer Res2008681697706\n1833984940LiaoCHYehCTHuangYHWuSMChiHCTsaiMMDickkopf 4 positively regulated by the thyroid hormone receptor suppresses cell invasion in human hepatoma cellsHepatology20125591020\n2199412941JungSHLeeAYimSHHuHJChoeCChungYJSimultaneous copy number gains of NUPR1 and ERBB2 predicting poor prognosis in early-stage breast cancerBmc Cancer201212382\n2293872142GuoXWangWHuJFengKPanYZhangLLentivirus-mediated RNAi knockdown of NUPR1 inhibits human nonsmall cell lung cancer growth in vitro and in vivoAnat Rec (Hoboken)2012295211421\n2296179843MuYYanXLiDZhaoDWangLWangXNUPR1 maintains autolysosomal efflux by activating SNAP25 transcription in cancer cellsAutophagy20181465470\n2913042644LiXMartinTAJiangWGCOM-1/p8 acts as a tumour growth enhancer in colorectal cancer cell linesAnticancer Res201232122937\n2249335345BakYShinHJBakIYoonDYYuDYHepatitis B virus X promotes hepatocellular carcinoma development via nuclear protein 1 pathwayBiochem Biophys Res Commun201546667681\n2639231546HanahanDWeinbergRAHallmarks of cancer: the next generationCell201114464674\n2137623047AndraeJGalliniRBetsholtzCRole of platelet-derived growth factors in physiology and medicineGenes Dev2008221276312\n1848321748ChenPHChenXHeXPlatelet-derived growth factors and their receptors: structural and functional perspectivesBiochim Biophys Acta20131834217686\n2313765849OngHSGokavarapuSTianZLiJXuQCaoWPDGFRA mRNA is overexpressed in oral cancer patients as compared to normal subjects with a significant trend of overexpression among tobacco usersJ Oral Pathol Med2017465917\n2834226450SahraeiMRoyLDCurryJMTeresaTLNathSBesmerDMUC1 regulates PDGFA expression during pancreatic cancer progressionOncogene201231493545\n2226684851PalomeroJVeglianteMCRodriguezMLEguileorACastellanoGPlanas-RigolESOX11 promotes tumor angiogenesis through transcriptional regulation of PDGFA in mantle cell lymphomaBlood2014124223547\n2509217652KeatingGMSantoroASorafenib: a review of its use in advanced hepatocellular carcinomaDrugs20096922340\n19228077 T3/TR signaling induces an increase in NUPR1 mRNA and protein expression.']","Figure 1 HepG2-TR, HepG2-TR, and HepG2-neo cells were treated with T (0-100 nM) for 6-48 h, and NUPR1 expression levels were measured using qRT-PCR. Expression of NUPR1 mRNA was normalized to that of 18s rRNA. NUPR1 protein expression levels were determined in TR overexpression and control HepG2 and J7 cell lines treated for different times with different concentrations of T . Lamin A/C was used as an internal control. Schematic representation of the NUPR1 promoter, indicating potential TREs (squares). HepG2-TR cells were transfected with serially deleted NUPR1 5'-flanking DNA pGL2-luc reporter constructs, and treated with 0-100 nM T . After 24 h, cells were lysed and promoter activity was determined. HepG2-TR cell lysates were immunoprecipitated with nonspecific mouse IgG or antibodies against TR. FURIN and GAPDH promoter regions were used as positive and negative controls, respectively. Differences were analyzed using a one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001).",yes
PMC3851716,Figure_6,oa_package/44/4f/PMC3851716.tar.gz,"['Luciferase reporter assays revealed that the FMRP S499A mutant which mimics the nonphosphorylated form of FMRP was not able to repress translation via the p0071 3 UTR (A).', 'Moreover, the FMRP S499A mutant failed to rescue cell morphology and actin filament organization when expressed in Fmr1 MEFs (B).', 'FMRP modulates actin organization by controlling p0071 expressionHaving shown that FMRP controls p0071 mRNA translation, we aimed at analyzing whether this regulatory role is essential for the FMRP-directed control of actin organization.']","FIGURE 6. FMRP controls p0071 protein synthesis and actin organization in a S499-dependent manner. ( ) Luciferase reporter assay with the indicated constructs (as described in A) showing that translational repression of the p0071 3 UTR depends on S499 of FMRP. Transfection of the FMRP S499A mutant in MEFs had no influence on luciferase activity. = 3, (*) 0.05. ( ) Immunofluorescence studies of MEFs overexpressing EGFP, EGFP-FMRP WT, or EGFP-FMRP S499A. Whereas the expression of FMRP WT promotes parallel organization of stress fibers and elongated cell morphology, expression of FMRP S499A fails to modulate actin organization or cell shape. Bars, 20 m.",yes
PMC5224436,Figure_4,oa_package/5a/08/PMC5224436.tar.gz,"['The head MRI (DWI, PWI) and CEMRA are shown in .', 'MRI-T2WI, Flair and DWI demonstrated a-c) high signal intensities in the right lateral medulla.']","Figure 4 MRI-T2WI, Flair and DWI demonstrated high signal intensities in the right lateral medulla. DSC-PWI demonstrated the lesion ROI low CBF, low CBV, decreased TTP, delayed MTT, and delayed Tmax on the VAH side. The representative rigid quantitative thresholds in the ROIs of 11 and 12 are shown in . CEMRA indicated basilar artery hypoplasia, right vertebral artery hypoplasia, and severe kinking of the left vertebral V1 segment. VAH - vertebral artery hypoplasia, CBF - cerebral blood flow, CBV - cerebral blood volume, TTP - time to peak, MTT - mean transit time",yes
PMC9699864,Figure_7,oa_package/c4/49/PMC9699864.tar.gz,[':Immediate postoperative MRI was done to rule out any compressive pathology.'],Figure 7: Immediate postoperative MRI was done to rule out any compressive pathology. (a) MRI showed T2 intramedullary hyperintensity of the cord at C3 level which appeared expanded with a central syrinx. (b) Cord edema was noted at the C3 level. No evidence of epidural hematoma or other compressive lesion at the operative site indicating reperfusion injury. The blue bar in the figure indicates the axial images with maximal intramedullary hyperintensity of the cord and the reperfusion injury which corresponds to C3 level. (c) Axial at C3 and C3/4 Levels showing whitening of the cord (White Cord Syndrome).,yes
PMC5780734,Figure_6,oa_package/ae/ea/PMC5780734.tar.gz,['Same image as figure 4 with the right infrahilar pneumonia indicated by arrows.'],Figure 6 Same image as with the right infrahilar pneumonia indicated by arrows.,yes
PMC7671569,Figure_2,oa_package/4a/75/PMC7671569.tar.gz,"[' 2).', 'b On ADC (apparent diffusion coefficients) map, the restriction of diffusion in the same location supports the acute onset of the stroke (arrow)DiscussionCoronavirus disease 2019 (COVID-19) infection is an ongoing pandemic, characterized by high morbidity and mortality that emerged from Wuhan (China) in December 2019.']","Fig. 2 MRI. Axial T2 weighted image shows hyperintense lesion in cerebellar vermis demonstrating an ischemic stroke (arrow). On ADC (apparent diffusion coefficients) map, the restriction of diffusion in the same location supports the acute onset of the stroke (arrow)",yes
PMC8630050,Figure_2,oa_package/31/fb/PMC8630050.tar.gz,"['2A).', '2B).', '2C), while we detected the presence of ataxin-2 aggregates in the Atx2MUT injected hemisphere (', '2D F).', '2K).', '2H J, L).', '2G), although it was lower than the marker loss reported upon an injection with PBS (ref.', 'Neuronal degeneration in different brain regions is typically correlated with behavioral manifestations.']","Fig. 2 The expression of mutant ataxin-2 in the striatum induces aggregation and loss of neuronal marker. Animals received a stereotaxic injection with lentiviral vectors (LVs) encoding for human wild-type ataxin-2 (Atx2WT) and mutant ataxin-2 (Atx2MUT) in left and right striatum hemisphere, respectively. Animals were divided in three groups according to the time of ataxin-2 expression, for 4-, 8- and 12- weeks after injection. Immunohistochemistry of brain sections revealed a general diffuse signal for ataxin-2 protein expression in Atx2WT hemisphere. In contrast, the overexpression of Atx2MUT resulted in ataxin-2 aggregates at 4 ( ), 8 ( ), and 12 ( ) weeks of experiment. The quantification of the total number of mutant aggregates showed a significant increase over time of ataxin-2 expression, reaching high levels at 12 weeks (values are expressed as meanSEM =3 per timepoint; * <0.05; one-way ANOVA followed by post hoc Tukeys multiple comparisons test). Immunohistochemistry for DARPP-32 neuronal marker showed that Atx2WT results in reduced marker loss, whereas Atx2MUT induces a higher depletion of staining for DARPP-32 either at 4- and 8-weeks post injection ( , ). These alterations are particularly high at 12 weeks ( , ) (values are expressed as meanSEM =3 (4-, 8-weeks) and =8 (12-weeks); * <0.05; one-way ANOVA followed by post hoc Tukeys multiple comparison test).",yes
PMC4657751,Figure_2,oa_package/9a/f7/PMC4657751.tar.gz,"['Striatal target structures, consisting of the left and right putamen and caudate, were segmented from the MPRAGE using the FreeSurfer suite of automated tools (see ).', 'org/1999/xlink"" xlink:href=""npjschz20146-f1""/>An axial view of striatal regions of interest including the putamen (yellow) and caudate (green).']",Figure 2 An axial view of striatal regions of interest including the putamen (yellow) and caudate (green).,yes
PMC6034140,Figure_3,oa_package/2d/db/PMC6034140.tar.gz,"['02) (Table 1 and ).', 'Radiographic appearance of the left foot.', '3.']","Fig. 3 Radiographic appearance of the left foot. A, CIA+OVX+CKI group. B, CIA+OVX+Veh group. C, CIA+Sham+CKI group. D, CIA+Sham+Veh group. The score in the CIA+sham+CKI group was significantly lower than that in the CIA+sham+Veh group. CIA=collagen-induced arthritis, OVX=ovariectomy, CKI=cathepsin K inhibitor, Veh=vehicle.",yes
PMC3099075,Figure_3,oa_package/c8/bc/PMC3099075.tar.gz,[],"Figure 3 Variably dilated biliary ductular channels surrounded by the dense fibrous tissue, collection of inflammatory cells and cords of hepatocytes (H and E, 100)",yes
PMC4865854,Figure_3,oa_package/c8/e6/PMC4865854.tar.gz,"['8 in protein levels of SIRT1 in Tg MJD mice, in comparison with WT littermate (a,b), was observed.', 'On CR, SIRT1 mRNA and protein levels were completely re-established to the levels observed in the non-diseased mice (a,b).', 'We observed a significant increase in LC3BII levels and a decrease in p62 levels in cerebella of MJD Tg mice fed with CR diet, in comparison with MJD TgAL- fed animals (Supplementary ).', 'org/1999/xlink"" xlink:href=""ncomms11445-f2""/>SIRT1 levels are abnormally reduced in the cerebellum of MJD Tg mouse model and CR re-establishes normal SIRT1 cerebellar levels.']","Figure 3 SIRT1 levels are abnormally reduced in the cerebellum of MJD Tg mouse model and CR re-establishes normal SIRT1 cerebellar levels. ( ) SIRT1 is compromised in the cerebellum of Tg MJD mice. A significant decrease of 58.48.3% in mRNA levels of SIRT1 was detected. These results were supported by western blotting in ( ). Data represent means.e.m.; NS >0.05 and * <0.05 compared with Tg-AL. ( , ) WT =5; Tg-AL =5; Tg-CR =4. SIRT1.1 (SIRT1 isoform 1) and SIRT1.2 (SIRT1 isoform 2). See also .",yes
PMC7433935,Figure_2,oa_package/52/07/PMC7433935.tar.gz,['The localization of septa was done at its point of origin from the sinus wall [].'],Figure 2 Localization of septa with respect to the wall of the sinus,yes
PMC5585498,Figure_7,oa_package/7a/2e/PMC5585498.tar.gz,['Clinical findings.'],"Figure 7 Clinical findings. A, Extensive swelling and ulceration of the mucosa of the hard palate covered by whitish pseudomembrane with an ill-defined margin. B, The involved anterior upper gingival mucosa with a slice-shaped neoplasm at the labial side. C, A well-bordered round skin erythema covered with yellowish crust at the area of the right brachial biceps.",yes
PMC10449997,Figure_5,oa_package/8f/66/PMC10449997.tar.gz,"[' 5a h), which indicates that both the antagonists are specific for the TRPC6 channel.', 'Effects of SAR7334 (SAR; 100 nM) and SH045 (100 nM) are observed in each TRPC6 mutantTo more directly explore the difference between TRPC6 wild-type, V691Kfs*, and P112Q mutants, we recorded whole-cell currents in transfected HEK293 cells [19].']","Fig. 5 Functional characterization of disease-related TRPC6 mutants in calcium influx measurements. Changes in intracellular calcium concentration ( / ) were measured in Fluo-4-AM-loaded HeLa cells expressing wild type (WT), P112Q, G757D, or V691Kfs* treated with doxycycline (Dox) (0.5g/mL) 24h before measurement and no Dox treated cells. Treatment with agonist carbachol (Cch: 100M): Cch was added about 20s after the start of the measurement. Effects of TRPC6 blockers SAR7334 (SAR; 100M) and SH045 (100M) are observed in each TRPC6 mutant. DOG (100M) was added about 20s after the start of the measurement. Effects of SAR7334 (SAR; 100nM) and SH045 (100nM) are observed in each TRPC6 mutant",yes
PMC5023734,Figure_3,oa_package/09/9b/PMC5023734.tar.gz,"[' 3e, f; p 0.', ' 3a) and 40 weeks post-injection (', ' 3b, d) but also to the host cortex (', ' 3c).', ' 3e h).', ' 3i).', ' 3j, k).', '', 'CTX cortex; DAPI 4 ,6-diamidino-2-phenylindole; DARPP-32 dopamine- and cAMP-regulated phosphoprotein, Mr 32 kDa; HD143F fibroblasts derived from an HD patient expressing 143 CAG repeats; hEF human embryonic fibroblasts; NeuN neuronal nuclei; STR striatum; w weeksImplanted HD fibroblasts lead to a progressive gliosis and inflammation within the host brainIn addition to the loss of DARPP-32+ medium spiny neurons and the formation of EM48+ mHtt, increased gliosis and inflammation are evident in HD.']","Fig.3 Evidence of cell-to-cell propagation of mHtt. Immunoflurorescent staining for mHtt (EM48; ), MW7 ( ) or ubiquitin ( ) combined with either DARPP-32 ( ), MAP2 ( ) or NeuN ( ) reveals the propagation of mHtt from implanted HDpatient-derived fibroblasts into both host striatal ( , , , ) and cortical cells ( , , , ) at 30 and 40weeks post-transplantation, as assessed by confocal microscopy. The atlas section of the murine brain illustrates areas where images were acquired ( ). Quantification of the relative intensity of the immnofluorescent EM48 staining further revealed that mHt aggregates were more abundant in the striatum than in the cortex ( ). Values are expressed as meanSEM. Statistical analyses were performed using the Students test. *** <0.001 compared to STR. The number of mice quantified in each group is indicated in each of the graphs. Triple immunofluorescence for EM48, DARPP-32 and DAPI in mice injected with hEF showed, as expected, the absence of mHtt aggregates ( , ). , =20m; , =50m; =10m; =20m; , =25m; , =20m). cortex; 4,6-diamidino-2-phenylindole; dopamine- and cAMP-regulated phosphoprotein, Mr 32kDa; fibroblasts derived from an HD patient expressing 143 CAG repeats; human embryonic fibroblasts; neuronal nuclei; striatum; weeks",yes
PMC9313121,Figure_1,oa_package/8b/95/PMC9313121.tar.gz,"['At the same time, it shows a mild to moderate presence of TDP-43 neuronal cytoplasmic inclusions in the amygdala and HiC and no -syn deposits (, mother s brain images: A, D, G, and J showing BG, SN, PL, and HiC, respectively).', 'No TDP-43 inclusions are detectable (, son s brain images: B, E-E , H, K, and L showing BG, SN, PL, and HiC, respectively).', 'Amyloid deposits are detectable only in the neocortex (A1), and TAU pathology is limited to the entorhinal cortex (B1), corresponding to a picture of low AD pathology (, control s brain images: C, F, I, and M, showing BG, SN, PL, and HiC, respectively).', '00116384684Comparison of the neuropathological picture among mother, son, and the control case.']","Figure 1 Comparison of the neuropathological picture among mother, son, and the control case. ( , ) the BG of mother and son show widespread diffuse and dense A plaques (asterisks) and physiological intracellular amyloid in some neurons (arrows) (4G8 antibody; magnification 10); ( ) absence of A in the BG of the control case (4G8 antibody; magnification 10). ( ) the SN of mother, son, and control, respectively (-SYN-KM51 antibody; magnification 20); only the son presents severe LTS with many LBs (( ); arrowheads); LBs are also easily detectable by using Hematoxylin &Eosin staining only (image ( ); magnification 20). ( ) the PL (cortex) of mother, son, and control, respectively; image G shows mothers picture, characterized by the presence of strong pTau deposits with NFT (arrows) and NP (asterisks), according to her Braak stage VI (AT8 antibody; magnification 4); image H demonstrates sons picture, characterized by LTS with many LBs (arrowhead), according to his Beachs stage IV (-SYN-KM51 antibody; magnification 10); image I shows the parietal cortex of the control case without any pathology (AT8 and -SYN-KM51 antibodies; magnification 10). ( ) the HiC of the three cases; image ( ) demonstrates mothers picture with NFT (arrows) and NP (asterisks) due to severe pTAU pathology (AT8 antibody; magnification 4); image ( ) shows sons neuropathology in the HiC, characterized by both LBs (arrowhead) and LNs (circles) (-SYN-KM51 antibody; magnification 40); image ( ) proves the contemporary presence of pTAU lesions in the HiC of the son with NFT (arrows) and NP (asterisks) (AT8 antibody; magnification 10); image ( ) proves the absence of pathology in the HiC of the control case (AT8 and -SYN-KM51 antibodies; magnification 4). The 1 cm calibration bar in the lower right corner of the figure applies to all images corresponding to 85 m in ( , , ); 47 m in ( , , , ); 194 m in ( , , ); 35 m in ( ); and 107 m in ( ).",yes
PMC7729318,Figure_2,oa_package/40/c1/PMC7729318.tar.gz,"['Consequently, the RNA expression of miR-29b was significantly elevated by mimics and reduced by inhibitors compared with the scramble (A, P 0.', 'Transfection of siRNA-CDC-7 significantly reduced mRNA expression of CDC-7 (B, P 0.', 'The proliferation of HUASMCs indicated with relative expression pf Ki67 and PCNA were significantly inhibited with miR-29b mimic and siRNA-CDC-7 while promoted by a miR-29b inhibitor (C,D, P 0.', 'EDU positive cells in HUASMCs were significantly decreased with siRNA-CDC-7 and miR-29b mimic while increased with miR-29b inhibitor (E,F, P 0.', 'Also, markers of VSMC phenotypic switching were tested, -SMA, and SM-MHC was induced with miR-29b mimic or siRNA-CDC-7, while MMP-2 was inhibited (C,G, P 0.', 'Expectedly, miR-29b mimic, and siRNA-CDC-7 significantly decreased the invasive cells per field and wound closure rate of HUASMCs, while miR-29b inhibitors promoted the migration of HUASMCs (H,I, P 0.', 'siRNA-CDC-7 and miR-29b mimic inhibit proliferation and migration of HUASMCs.']","Figure 2 siRNA-CDC-7 and miR-29b mimic inhibit proliferation and migration of HUASMCs. HUASMCs were transfected with siRNA-CDC-7, miR-29b scramble, miR-29b mimic, or miR-29b inhibitor, then kept for another 24 hours for later experiments. (A) miR-29b mRNA expression in different groups determined by RT-PCR. **, P<0.01, compared with the miR-29b scramble group. (B) Relative RNA expression of HUASMCs transfected with scramble and siRNA-CDC-7. *, P<0.05, compared with the miR-29b scramble group. (C) Relative protein levels of Ki67, PCNA, -SMA, SM-MHC, and MMP-2 were evaluated with western blotting. (D) Ki67 and PCNA levels were quantified with Image J, *, P<0.05, compared with the miR-29b scramble group. , P<0.05, compared with the miR-29b mimic group. (E) Cell proliferation was detected by EdU staining. Cells in red fluorescence represented the positive cell stained with EdU, and those in blue were stained with DAPI. (F) EdU positive cell count. *, P<0.05, compared with the miR-29b scramble group. (G) Relative protein levels of -SMA, SM-MHC, and MMP-2 quantified with Image J. *, P<0.05, compared with the miR-29b scramble group. (H) Transwell tests detected cell migration, and (I) the wound healing of the transfected cells. MMP-2, matrix metalloprotein-2; -SMA, alpha-smooth muscle actin; SM-MHC, smooth muscle Myosin heavy chain 11; miR-29b, microRNA-29b; HUASMCs, human umbilical artery smooth muscle cells; RT-PCR, real-time polymerase chain reaction; EdU, 5-Ethynyl-2-Deoxyuridine; DAPI, 4,6-diamidino-2-phenylindole.",yes
PMC10635633,Figure_2,oa_package/d1/c0/PMC10635633.tar.gz,['\n\nA: a single superficial mucocele on the left side of the soft palate in a 26-years old man without local or systemic disease.'],Figure 2 A: a single superficial mucocele on the left side of the soft palate in a 26-years old man without local or systemic disease. B: multiples superficial mucocele in on the soft palate measuring 2 - 4mm in a patient with Sjgrens Syndrome. C: a single superficial mucocele measuring 7mm in on the border of the tongue of a patient with an erythematous lupus patient.,yes
PMC5704837,Figure_3,oa_package/84/bf/PMC5704837.tar.gz,['Serial images of brain magnetic resonance imaging.'],Figure 3 Serial images of brain magnetic resonance imaging. (A) Newly found brain metastasis in 2013. (B) Improvement of brain metastasis after chemotherapy. (C) Residual brain metastasis in 2014. (D) Follow-up image in 2015 after whole brain radiotherapy in 2014 (red arrows indicate the lesions).,yes
PMC4156302,Figure_1,oa_package/93/37/PMC4156302.tar.gz,"['ResultsMorphometric analysis of knee joint articular cartilage from non-operated Mc1re/e and WT mice\nA depicts representative Safranin O/fast green stained image from a frontal section through a mouse knee joint.', 'Articular cartilage morphology was assessed by morphometric evaluation of Safranin O stained area of knee joints of non-operated 11 weeks (B, C) and 6 months old animals (', 'g001Histomorphometric comparison of knee joint morphology between non-operated Mc1re/e and WT mice.']",10.1371/journal.pone.0105858.g001,yes
PMC7499545,Figure_3,oa_package/87/a0/PMC7499545.tar.gz,['12-lead ECG showing complete AV block (Type 3) with junctional escape rhythm.'],Figure 3 12-lead ECG showing complete AV block (Type 3) with junctional escape rhythm.,yes
PMC11483923,Figure_5,oa_package/3e/20/PMC11483923.tar.gz,[],"FIGURE 5 Effect of antimicrobial agents on phosphorylation of S6RP and Akt in differentiated hamster sebocytes. Hamster sebocytes were treated with ozenoxacin, nadifloxacin, and clindamycin in the presence or absence of insulin (a, b) and 5dihydrotestosterone (5DHT) (c, d), as shown in Figures and , respectively. Cell lysates were prepared and intracellular pS6RP, pAkt, and tubulin levels were measured as described in the Materials and Methods. Data are presented as meansstandard deviation of quadruplicate wells. * <0.05 compared with the nontreated group (Student or AspinWelch's test). <0.05 compared with insulin +10 N NaOH, or 5DHT+10 N NaOH (Tukey's multiple comparison test). <0.05 compared with insulin+ozenoxacin 10mol/L, or 5DHT+ozenoxacin 3mol/L (Tukey's multiple comparison test). <0.05 compared with insulin+ozenoxacin 100mol/L, or 5DHT+ozenoxacin 10mol/L (Tukey's multiple comparison test).",yes
PMC11305768,Figure_3,oa_package/7e/02/PMC11305768.tar.gz,[],Figure 2 Sagittal view. Pelvic MRI showing slowly progressive residual/recurrent disease consisting of a 43cm multiloculated cystic lesion containing fat in the pouch of Douglas (red arrow). A stable 8mm subcapsular deposit overlying segment 7 of the liver was also notednot seen on this image.,yes
PMC2442195,Figure_1,oa_package/f8/47/PMC2442195.tar.gz,"['In the absence of BTX, levels of surface AChR were similar at 0 and after 6 h of chase ( A, histogram; gray bars); incubation of CHO-K1/A5 cells for 6 h with a saturating concentration of BTX resulted in a 40% reduction in surface AChR levels (', '.', 'In C2C12 cells treated with BTX, surface levels of AChR were found to decrease significantly ( B), indicating that both C2C12 and CHO-K1/A5 cells exhibit BTX-induced down-modulation of AChR.']","Figure 1. (A and B) CHO-K1/A5 (A) or C2C12 cells (B) were incubated on ice without (BTX) or with BTX (+BTX) and chased at 37C for 0 or 6 h in the absence or presence of the toxin. At the end of the incubation, surface levels of AChR were quantified by measuring the extent of anti-AChR mAb 210 binding to surface receptors. The bars in the top (CHO-K1/A5) and bottom histograms (C2C12) represent normalized fluorescence intensity of secondary AlexaFluor568 antibody directed against the primary anti-AChR monoclonal antibody mAb 210 versus that measured in untreated (toxin free) controls. In the case of C2C12 cells, AlexaFluor488-labeled BTX was used for inducing internalization, and the quantification represents the ratio of mAb 210/BTX fluorescence at 0 and 6 h, further normalized to ratio at 0 h. Each bar corresponds to weighted mean SEM (error bars) obtained from two independent experiments, with 150 cells for CHO cells and 25 cells for C2C12 cells. *, P > 0.1; **, P < 0.001; ***, P < 0.0001. Bars, 10 m.",yes
PMC11128201,Figure_5,oa_package/77/4d/PMC11128201.tar.gz,"['Echocardiographic examination demonstrated that MI/R-induced decrease of cardiac systolic function (EF% and FS%) was attenuated in Hspa12aki mice compared with WT mice (Supplemental A).', 'Moreover, MI/R increased cardiomyocyte apoptosis in both genotypes, but apoptosis was alleviated in Hspa12aki mice relative to WT (Supplemental B).', 'To determine if maintenance of aerobic glycolytic homeostasis mediates the cardioprotective effect of HSPA12A, glycolysis was blocked with 2-Deoxy-D-glucose (2-DG) or oxamate (Oxa) in NRCM during reoxygenation as described previously (15, 28) (A).', 'Remarkably, when 2-DG removed the HSPA12A-induced increase of extracellular lactate (B), the protective effects of HSPA12A against H/R injury, as indicated by attenuated LDH release and morphological abnormalities, were also reversed by 2-DG (, C and D).', 'Moreover, 2-DG abolished a HSPA12A-mediated increase of Bcl-2 protein levels in H/R NRCM (E).', 'Similarly, treatment with Oxa ablated a HSPA12A-mediated increase of extracellular lactate and decrease of LDH release (, B and C) in H/R NRCM.', 'When Oxa was used to prevent the increase of lactate production in H/R-treated Hspa12aO/E NRCM (B), the increase of H3K56la level was also abolished (B).', 'Glycolysis inhibition abolished the cardioprotection of HSPA12A against H/R challenge.']","Figure 5 Glycolysis inhibition abolished the cardioprotection of HSPA12A against H/R challenge. ( ) Brief scheme of glycolytic inhibitor treatment. 2-Deoxy-D-glucose (2-DG) and oxamate (Oxa) were introduced to NRCM culture during reperfusion. ( ) Extracellular lactate levels were examined in culture medium. = 3 6/group. ( ) LDH leakage was examined in culture medium. = 7 for NC group and for group, = 3 for + 2-DG group and for + Oxa group. ( ) Morphological alterations were examined using a phase-contrast microscope; total original magnification, 200 . Scale bars: 100 m. = 5/group. ( ) The indicated gene expression was examined by immunoblotting analysis. = 7 for NC group and for group, and = 3 for + 2-DG group. Data are mean SD. *** 0.001 and ** 0.01 using ordinary 1-way ANOVA followed by post hoc test.",yes
PMC8395035,Figure_8,oa_package/64/9c/PMC8395035.tar.gz,"['Primers for Vps35-mCherry and endogenous Vps35 were generated as indicated in A.', 'Using primers for Vps35-mCherry, Vps35-mCherry s mRNAs were detected in neocortex and hippocampus of TgVps35Neurod6 and TgVps35Neurod6; KO mice, but not in control and Vps35Neurod6 mice, suggesting the specificity (B).', 'However, the Vps35 mRNA levels were slightly higher in TgVps35Neurod6; KO than in Vps35Neurod6 mice (B).', 'Further quantification analysis showed that the exogenous Vps35-mCherry s mRNAs were at levels of about 4 6% over the total Vps35 mRNA (B), suggesting a low level of Vps35-mCherry s expression.', 'As shown in C, the Vps35 signal was detected in the neocortex of control, Vps35Neurod6 and TgVps35Neurod6; KO mice.', 'Consistent with the result of qRT-PCR, the signal was markedly reduced in Vps35Neurod6 mice, with a slight increase in TgVps35Neurod6; KO brain, as compared with that of Vps35Neurod6 mice (C,D).', 'No significant change was observed between the control and Vps35Neurod6 mice, as well as the TgVps35Neurod6; KO mice at P14 (E), suggesting the Vps35 effect on the protein stability of Vps26a and Vps29, but not their transcription.', 'Low level of Vps35-mCherry s expression.']","Figure 8 Low level of s expression. ( ) Schematic graph illustrating the primers design for real-time RT-PCR (qRT-PCR) analysis. ( ) Quantification of and mRNA expression in control, , and brains ( = 3 animals per genotype). ( ) Representative images of RNA scope analysis of mRNAs (green) in control, and cortex. The nuclei were stained with DAPI (blue). ( ) Quantification analysis of data in ( ) ( = 3 animals per genotype; one-way ANOVA with Tukeys multiple-comparison test). ( ) Quantification of and s mRNA levels ( = 3 animals per genotype; one-way ANOVA with Tukeys multiple-comparison test). Scale bars as indicated in each panel. Individual data points were shown as dots with group mean S.E.M; * < 0.05; ** < 0.01; **** < 0.0001; n.s.: Not significant.",yes
PMC9746542,Figure_5,oa_package/8b/ea/PMC9746542.tar.gz,['.'],"Figure 5. Alteration of the cytokines levels in the hippocampus and serum following Bacillus Calmette Guerin (BCG) treatment. The normalized and analyzed levels of TNF-, IL-4, and IFN- in serum (a) and hippocampus (b); 1, 2, and 3 represents APP/PS1 mice injected with BCG once, twice, and three times, respectively. In serum, BCG vaccination up-regulated IL-4 and IFN- expression, and down-regulated TNF- expression, and changes in the levels of these cytokines in the hippocampus were almost consistent with those in serum. Data were analyzed by performing Students -test and the results obtained are presented as the meanSEM normalized to the controls. =6 for each group. * <.05, ** <.01 and # <.001 vs. vehicle control.",yes
PMC8693238,Figure_2,oa_package/0b/0a/PMC8693238.tar.gz,"['Imaging to assess scoliosis revealed osteoporosis, bilateral coxa vara, and diffuse platyspondyly without scoliosis ().', '.']","Figure 2. Skeletal features associated with MOPDII. Lateral ( ) and antero-posterior ( ) X-ray of the spine showing osteoporosis, bilateral coxa vara, and diffuse platyspondyly without scoliosis.",yes
PMC10362984,Figure_1,oa_package/39/1f/PMC10362984.tar.gz,"['A computed tomography (CT) scan (s 1(a) and 1(b)) of the abdomen and pelvis with intravenous (IV) contrast demonstrated a distended stomach with fluid and the presence of peripheral gas along the anterior and lateral walls of the gastric fundus and proximal gastric body, suggesting pneumatosis.', 'A repeat CT scan of the abdomen with oral contrast (s 1(c) and 1(d)) on day four showed a decrease in gastric pneumatosis, with some residual pneumatosis at the fundus.', '0-8490800504225219954Coronal (a) and axial (b) images from the initial CT of the abdomen and pelvis with IV contrast demonstrate a distended stomach with pneumatosis (yellow arrows) within the gastric wall involving most of the stomach.']","Figure 1 Coronal (a) and axial (b) images from the initial CT of the abdomen and pelvis with IV contrast demonstrate a distended stomach with pneumatosis (yellow arrows) within the gastric wall involving most of the stomach. No pneumoperitoneum, portal venous gas, or pneumatosis within the small or large bowel was identified. Coronal (c) and axial (d) images from CT of the abdomen and pelvis with IV and oral contrast taken on day four demonstrating oral contrast and an enteric tube (white arrow) within the stomach with near interval resolution of gastric pneumatosis. Residual pneumatosis in the wall of the anterior antrum (yellow arrows) is noted.",yes
PMC11389653,Figure_2,oa_package/45/d8/PMC11389653.tar.gz,['Ultrasound.'],Figure 2 Ultrasound. Grayscale image demonstrating a complex solid and cystic mass in the left subareolar region (blue arrows).,yes
PMC6993326,Figure_1,oa_package/a9/5c/PMC6993326.tar.gz,"[' 1); the physical and neurologic exams, as well as repeat EEGs revealed no pathologies.', 'CT before pacemaker implantationThere were no syncope provoking factors.', '1).']",Fig. 1 CT before pacemaker implantation,yes
PMC10863782,Figure_2,oa_package/4f/eb/PMC10863782.tar.gz,['Need for cardiac evaluation.'],Figure 2 Need for cardiac evaluation. Sankey diagram showing the need for cardiac evaluation according to AHA2012 (53%) and AHA2022 (73%). AHA indicates American Heart Association.,yes
PMC3419745,Figure_7,oa_package/e9/0b/PMC3419745.tar.gz,"['/g (; Table S2).', 'Brevetoxin was also identified in all other sample types (kidney, blood, lung, cerebellum) except spleen (as determined by the RBA; ).', 'g007Brevetoxin concentrations in various sample types from stranded bottlenose dolphins in the 2004 UME.', 'In fact, of the primary samples analyzed and illustrated in (blood, feces, gastric fluid, liver, stomach contents, urine), only 6 samples were dl for brevetoxin.']",10.1371/journal.pone.0042974.g007,yes
PMC7596178,Figure_2,oa_package/74/e3/PMC7596178.tar.gz,"['05; C].', 'ki20227-induced morphological changes in the microglia following reperfusion after ischemia.']","Figure 2 ki20227-induced morphological changes in the microglia following reperfusion after ischemia. Representative confocal images of the microglia [ , green fluorescent protein (GFP)-positive microglia] in different groups. , 50 m. Quantification of microglial density showing a significant difference between groups. = 6 mice per group; N.S.: < 0.05, ** < 0.01, = 6. Quantification of microglial soma size showing a significant difference between groups ( = 6 mice per group). N.S.: < 0.05, * < 0.05, ** < 0.01, = 6. Representative confocal images of microglial proliferation ( , GFP-positive microglia; , BrdU-positive cells) in different groups. , 50 m. Quantification of microglial proliferation showing a significant difference between groups ( = 6 mice per group). ** < 0.01, = 6. Representative confocal images of the microglia ( , GFP-positive microglia) and skeletonized microglia in the vehicle-treated sham, ki20227-treated sham, vehicle-treated stroke, and ki20227-treated stroke mice. The image sizes ( ) in panels are of the same length. , 10 m. Analysis of the total number of microglial branch endpoints and the total length of microglial processes in all groups. Note that there was a significant difference between the vehicle-treated and ki20227-treated groups. The total number of microglial branch endpoints and the total length of microglial processes were significantly decreased after treatment with ki20227 on day 3 after global ischemia. * < 0.05, ** < 0.01, = 6.",yes
PMC11268916,Figure_9,oa_package/a1/ab/PMC11268916.tar.gz,"['In addition, SARA has devastating, long-term health and economic drawbacks for the dairy cow industry such as swinging and depression in feed intake, lowered diet digestion effectiveness, decreased milk fat %, lowered milk production, abscessation of the hepatic parenchyma and lameness ().', '.']",Fig. 9. Postmortem findings of multiple liver abscesses in a dairy cow.,yes
PMC5634503,Figure_1,oa_package/91/a4/PMC5634503.tar.gz,"[' 1A).', ' 1A, lanes 1 3 vs.', ' 1B)30.', ' 1A, lanes 1 3 vs.', ' 1B), a surprising observation since iron deficiency is expected to increase the turnover of ferritin by activating autophagy21.', ' 1A, lanes 1 6).', ' 1A, lanes 1 3 vs.', ' -Syn inhibits the degradation of ferritin and LC3II: ', '\nTo resolve the above dichotomy, further experiments were performed in vitro in a RPE cell line that expresses abundant -syn5,6,31.', ' 1C, lane 3 vs.', ' 1E).', ' 1D, lane 3 vs.', ' 1F), indicating blockage of ALP22.', ' 1G, lanes 1 2).', ' 1G, lanes 3 4).', ' 1D).', ' 1A (', ' 1D above (', ' 1G) has been confirmed in previous reports where proteins extracted from this band were subjected to Western blotting followed by probing with antibody specific to ferritin, and by transferring the proteins under native conditions and probing transferred proteins for ferritin33 35.']","Figure 1 -Syn inhibits the degradation of ferritin and LC3II: Fig.1: ( ) Representative Western blot of retinal lysates from -syn and -syn mice (lanes 13 vs. 46) shows expression of ferritin, LC3, -syn, RPE65 , and NCOA4 . ( ) Quantification by densitometry of ferritin and LC3II in -syn relative to -syn samples. ( ) Lysates of RPE 47 cells transfected with siRNA for -syn, scrambled siRNA, and non-transfected controls were analyzed by Western blotting. Representative image shows expression of ferritin, LC3, RPE 65, NCOA4, -syn and -actin. ( ) Expression of ferritin, LC3, NCOA4, -syn and -actin in non-transfected and RPE 47 cells stably expressing vector or -syn. ( ) Quantification of ferritin and LC3II expression by densitometry following knockdown of -syn. ( ) Quantification of ferritin and LC3II following over-expression of a-syn. ( ) Fe-feriitin in vector and -syn expressing RPE 47 cells pulsed with FeCl for 4h (lanes 1 & 2) and chased for 0h and 24h. Western blotting of same volume of samples as a control for protein loading. n=3 for all experiments. All values were normalized to -actin that served as an internal control, and represent meanSEM of the indicated (** <0.01, **p<0.01 *** <0.001).",yes
PMC3195806,Figure_4,oa_package/d6/91/PMC3195806.tar.gz,"['The patient underwent a right supraclavicular incision and surgical ligation of the ARSA with right subclavian carotid transposition using an end-to-side anastomosis; see s 4 and 5.', '003""/>Lateral view via a right supraclavicular incision: note that from above, the right common carotid artery, the right internal jugular vein, and the retroesophageal subclavian artery.']","Figure 4 Lateral view via a right supraclavicular incision: note that from above, the right common carotid artery, the right internal jugular vein, and the retroesophageal subclavian artery.",yes
PMC9395532,Figure_4,oa_package/d9/86/PMC9395532.tar.gz,"[' 4a).', ' 4a), and their proteolytic susceptibility was unaffected by the presence of detergent, akin to the lysosomal luminal protein cathepsin-L and neuroserpin a non-lysosomal secreted protein (', ' 4a).', ' 4a).', 'Extracellular Syn species in cerebrospinal fluid and in primary neuron media are not membrane-enveloped.', 'The pathogenic Syn species were also detected in the medium of Tgx2- SynA53T neurons collected at DIV49, but were not yet present at DIV35 or in the media of DIV49 Syn / and WT neurons (Supplementary ', ' 4b), indicating that it is not membrane-enveloped.', ' 4b).', ' 4b).']","Fig. 4 Extracellular Syn species in cerebrospinal fluid and in primary neuron media are not membrane-enveloped. Cerebrospinal fluid (CSF) collected from 6-month-old Tg-Syn mice were subjected to limited proteolysis using indicated concentrations of proteinase K (PK) in the absence or presence of 0.1% Triton X-100. Immunoblots for Syn protein levels (Syn , Syn , and Syn ), mature cathepsin-L (Cath-L ), TSG101, and neuroserpin ( =5 for Syn , Syn , TSG101; =4 for Syn , Cath-L ). Media collected from Tg -Syn neuron cultures at DIV49 was subjected to limited proteolysis in presence or absence of 0.1% Triton X-100 and analyzed as in ( =3). All data represent meansSEM. Each n is an independent collection (CSF or medium) and proteolysis experiment. n.s. not significant; * <0.05; **** <0.0001 by 2-way ANOVA with Bonferroni multiple comparisons post-test (compared at each PK concentration).",yes
PMC11331705,Figure_3,oa_package/1f/93/PMC11331705.tar.gz,"['Subsequent CT scan with a 3D bone reconstruction confirmed the diagnosis by showing the absence of the right collar bone; associated with scoliosis (, ).', 'Posterior view showing the skull base and spine which appear to be normal.']","Fig. 3 Advanced 3D bone reconstruction model showcasing a case of right cleidocranial dysplasia and highlighting the missing collarbone on the right side. It also shows the pacemaker with its ventricular lead on the right, and the port-a-catheter on the left.",yes
PMC3027001,Figure_1,oa_package/e8/95/PMC3027001.tar.gz,"['Microscopically, a poorly differentiated carcinoma was present within a sun-damaged dermis, infiltrating amongst dermal tattoo pigment as single cells and nests (s 1 4).', ""1JacobCITattoo-associated dermatoses: a case report and review of the literatureDermatologic Surgery20022810962965124106852GoldenbergGPatelSPatelMJWillifordPSanguezaOEruptive squamous cell carcinomas, keratoacanthoma type, arising in a multicolor tattooJournal of Cutaneous Pathology20083516264180959973McQuarrieDGAn unusual breast cancer: squamous cell carcinoma arising in a tattooMinnesota Medicine19664979980159327824PitarchGMart nez-Mench nTMart nez-AparicioAS nchez-CarazoJLMu ozDForteaJMSquamous cell carcinoma over tattoosJournal of the American Academy of Dermatology200756610721073175047285FragaGRProssickTATattoo-associated keratoacanthomas: a series of 8 patients with 11 keratoacanthomasJournal of Cutaneous Pathology20103718590193025726KlugerNMinier-ThouminCPlantierFKeratoacanthoma occurring within the red dye of a tattooJournal of Cutaneous Pathology2008355504507179762097OrtizAYamauchiPSRapidly growing squamous cell carcinoma from permanent makeup tattooJournal of the American Academy of Dermatology200960610731074194673848KlugerNDurandLMinier-ThouminCPseudoepitheliomatous epidermal hyperplasia in tattoos: report of three casesAmerican Journal of Clinical Dermatology200895337340187176109SarmaDKeratoacanthoma should be reported as 'Well differentiated squamous cell carcinoma, keratoacanthoma type': a Dermatopathologist's viewThe Internet Journal of Dermatology20075110GonADSMinelliLMeissnerMCGKeratoacanthoma in a tattooDermatology Online Journal2009157, article 911KlugerNPlantierFPseudo-epitheliomatous hyperplasia, keratoacanthoma, and squamous cell carcinoma occurring within tattoos: diagnostic issuesJournal of the American Academy of Dermatology200757590190217939943Low-power examination revealed abnormal keratin, severe dermal sun damage, tattoo pigment, and a cellular dermal neoplasm.""]","Figure 1 Low-power examination revealed abnormal keratin, severe dermal sun damage, tattoo pigment, and a cellular dermal neoplasm.",yes
PMC11328338,Figure_1,oa_package/4a/88/PMC11328338.tar.gz,[],"FIGURE 1 PhosphoTDP inclusion pathology in C9ALS visual cortex. Four unique study cases are shown, each with pTDP inclusion pathology in the form of long, thick neurites. Neuronal cytoplasmic inclusions were less commonly seen. Brodmann areas (BA) 18 and 17 are shown with both immunofluorescence (top row) and immunohistochemical (bottom row) preparations. All images were taken at 600 and the scale bar in the top left image applies to each of the four main pTDP panels. The inset of the top right panel shows dense, polyGApositive perinuclear inclusions in layer IVc of the same patient. The FITC (green) channel signal in the composite image represents autofluorescence only (e.g., lipofuscin pigment).",yes
PMC3688603,Figure_2,oa_package/ae/c2/PMC3688603.tar.gz,"['Analysis of transfected cells by Western blotting (A) revealed that the WT protein was efficiently expressed with either construct.', 'The results were confirmed by immunofluorescence of cells co-transfected with the same SYN1 constructs and a control pEGFP vector (B).', 'In addition, in cells positive for W356 Syn I expression, the protein formed small perinuclear aggregates (B).', 'g002The W356 Syn I variant is poorly expressed in transfected HeLa cells.']",10.1371/journal.pone.0067724.g002,yes
PMC7713670,Figure_8,oa_package/af/74/PMC7713670.tar.gz,"['8a-c).', 'Scale bars = 50 mDiscussionThis study is the first to evaluate the effect of TMEV infection and seizures on the activation state of NG2 cells using IHC techniques.']",Fig. 8 NG2 cell proliferation is not significantly changed in the cortex of TMEV-injected animals at both 4 and 14 dpi. NG2 cells (green) and proliferation marker Ki-67 (red) in the cortex of PBS-injected (top) and TMEV-injected (bottom) mice at 4 and 14 dpi. Arrow heads point to cells containing pixels colocalized for both NG2 and Ki-67. There is no significant change in colocalization of NG2 and proliferation marker Ki-67 in the cortex of TMEV-injected animals at 4 dpi. There is also no significant change in colocalization of NG2 and Ki-67 in the cortex of TMEV-injected animals at 14 dpi. Images are 20 maximum-intensity projections of 12m confocal z stacks. Scale bars = 50m,yes
PMC5583537,Figure_1,oa_package/28/57/PMC5583537.tar.gz,['The figure showing cross-section of coronary artery immediately after implantation of a bare metal stent black dots represent the stent struts (red arrow) (A); the figure showing significant in-stent restenosis with neointimal hyperplasia (red star) 6 mo after the implantation of a bare metal stent (B).'],Figure 1 The figure showing cross-section of coronary artery immediately after implantation of a bare metal stent black dots represent the stent struts (red arrow) (A); the figure showing significant in-stent restenosis with neointimal hyperplasia (red star) 6 mo after the implantation of a bare metal stent (B).,yes
PMC9374297,Figure_1,oa_package/9b/7c/PMC9374297.tar.gz,"[' 1) and MMAD (', ' 1 and 2.', 'Response surfaces (A, C, E) and contour plots (B, D, F) showing effect of process variables on particle size Vd.', 'Images representing 3D response surface (A, C) and contour plots (B, D) showing effect of process variables on MMAD.']","Fig. 1 Response surfaces ( , , ) and contour plots ( , , ) showing effect of process variables on particle size V . ( , ) Aspiration Flow rate. ( , ) Feed concentration Aspiration. ( ) Feed concentration flow rate. ( ) Normal % probability externally studentized residuals. ( ) Correlation between predicted and actual values of V .",yes
PMC9137166,Figure_3,oa_package/ff/1f/PMC9137166.tar.gz,"[' 3 shows the randomly selected samples of pleural effusion with different severities.', 'a No effusion; b Small effusion; c Moderate effusion; d Large effusionLabel verificationThe labels extracted from the radiology report based on a fixed rule search were compared with those obtained from the radiologist s itemized report inspection, and the deviation rate was 6.']",Fig. 3 Examples of X-rays of pleural effusions of varying severity. No effusion; Small effusion; Moderate effusion; Large effusion,yes
PMC8633007,Figure_1,oa_package/9e/eb/PMC8633007.tar.gz,"[' 1a, b), is a prime target for neutralizing antibodies that are induced by infection or by currently used COVID-19 vaccines10.', ' 1b).', ' 1c), many of which do not affect or even enhance human ACE2 receptor binding but may be detrimental for antibody recognition.', 'SARS-CoV-2 mutant binding to ACE host receptor quantified by atomic force microscopy and molecular dynamics simulation.', 'Previously, we have reported the use of atomic force microscopy (AFM) to map the interaction forces between the AFM tips functionalized with the S-glycoprotein of RBD wildtype (WT) and the ACE2 receptors on model surfaces.', ' 1c e).', ' 1d).', ' 1e)16,17.', ' 1a d and 6a were created with BioRender.', ' 1 are available at the RCSB protein database under the following references 6VW1 and 6VXX.']","Fig. 1 SARS-CoV-2 mutant binding to ACE host receptor quantified by atomic force microscopy and molecular dynamics simulation. Schematic of a SARS-CoV-2 virus particle, expressing at its surface the spike glycoprotein (S) that mediates the binding to host cells. The S glycoprotein is composed of two subunits, S1 and S2, and is commonly represented as a sword-like spike. The Protein Data Bank (PDB) model of this glycoprotein (ID: 6VXX) reveals how the subunits are comprised of different regions that are fundamental to the infection process. Other Structural studies (PDB ID: 6VW1) have previously obtained a complex between the receptor-binding domain (RBD, a subunit of the S glycoprotein) and the angiotensin-converting enzyme 2 (ACE2) receptor. Featuring a 3D rendering of SARS-CoV-2, this panel showcases the key spike protein mutations in the RBD domain on each of the studied SARS-CoV-2 variants of concern: Alpha, Beta, Gamma, and Kappa. Schematic of probing RBD mutant binding to ACE2 receptors using atomic force microscopy (AFM). RBD is covalently attached to the AFM tip via a heterobifunctional PEG-linker and their binding to ACE2 receptors immobilized on a gold (Au) coated surface is probed. Pixel-for-pixel force distance (FD) curve-based AFM approaches and retracts the tip of an AFM cantilever from the sample to record interaction forces, , over the tip-sample distance in FD curves. Forcetime curve from which the loading rate (LR) can be extracted from the slope of the curve just before bond rupture (LR= / ) (upper curve). The contact time refers to the time when the tip and surface are in constant contact (middle curve). The lower curve shows no binding event. The tip approach is highlighted in blue, and tip retraction in red. Representation of the system used in MD simulations.",yes
PMC11563982,Figure_3,oa_package/9f/98/PMC11563982.tar.gz,[],"Figure3 Representative images during CT imaging evaluation. , regions of interest (ROI) delineated in lesion and aorta respectively. , iodine concentration of the lesion and aorta on the iodine map. , soft tissue opacity at the pleural end of the PT (arrow). , tumor protrusion (arrow head), defined as triangular opacities extending from the tumor margins to PT. , cord-like PT (arrow head), defined as a PT thicker than 2 mm.",yes
PMC11627106,Figure_3,oa_package/0f/2f/PMC11627106.tar.gz,"['23 As shown in A C, the expression of iNOS, a M1-type polarization marker, was observably increased in the brains of SAMP8 mice when compared with those of SAMR1 controls (P 0.', '05, C).', '05) in the brains of SAMP8 mice, whereas TREML2 knockdown reversed these decrements (A and B, for ARG1: increased by 55.', 'The effects of TREML2 on microglial polarization in the brains of SAMP8 mice.']",Figure 3 The effects of TREML2 on microglial polarization in the brains of SAMP8 mice. The mRNA expression of M2-type microglial polarization markers ARG1 and CD206 as well as M1-type microglial polarization marker iNOS in the brains of mice were measured using RT-PCR. Data were analyzed by one-way analysis of variance followed by Tukeys post hoc test. Columns represent mean SD. N = 8 per group. * <0.05.,yes
PMC6334468,Figure_1,oa_package/a2/20/PMC6334468.tar.gz,"[' 1a, b).', '1c, d).', '1e, f).', 'Axial CT of case 1 shows a mass with distinct border in the right upper anterior mediastinum (a), which presents an obviously heterogeneous enrichment after enhancement (b).', '95)Morphology and immunophenotype of the tumorsMicroscopic examination of the core needle biopsy of case 1 showed a few large round or ovoid cells scattered in the inflammatory background composed of lymphocytes, plasma cells and histocytes.']","Fig. 1 Axial CT of case 1 shows a mass with distinct border in the right upper anterior mediastinum ( ), which presents an obviously heterogeneous enrichment after enhancement ( ). Axial plain ( ) and enhanced ( ) CT of case 2 reveal that in the right posterior mediastinum, there is a giant lobulated shadow with moderately heterogeneous enhancement. Sagittal ( ) and axial ( ) positron emission tomography and CT of case 3 indicate a mass in the middle mediastinum with avid fluorodeoxyglucose uptake (SUVmax=11.95)",yes
PMC9499725,Figure_8,oa_package/e4/f0/PMC9499725.tar.gz,[],Figura 4 Fotografia intraoperatria da ponte vascular da cartida direita por interposio da veia safena magna. Nota-se arteriorrafia na cartida interna no local onde foi posicionada a parte distal do vascular.,yes
PMC10713242,Figure_17,oa_package/6b/47/PMC10713242.tar.gz,[],"Figure 17 Thymic lymphoid lesions other than MALT lymphoma. In contrast to lymphoma, (A) thymic lymphoid hyperplasia, multilocular thymic cyst and LESA-like thymic hyperplasia show appropriate compartmentalization of T-cells in interfollicular areas and B-cells in lymphoid follicles (CD3-brown, CD20-red) (40). (B) CD21 highlights preserved follicular dendritic cell meshworks (40). (C) bcl-6 is positive in preserved reactive germinal centers (100). (D) bcl-2 is negative in germinal centers and positive in mantle zone cells and interfollicular T-cells (100). (E,F) Pan-cytokeratin highlights entrapped and/or distorted epithelium without complete effacement of the epithelial network or lymphoepithelial lesions (20 and 100, respectively). MALT, marginal zone lymphoma of mucosa-associated lymphoid tissue; LESA, lymphoepithelial sialadenitis.",yes
PMC9316148,Figure_6,oa_package/fe/e3/PMC9316148.tar.gz,"['05) by ERK5 knock-down, we observed 37 mouse transcription factors [39], with five of them being strongly affected (absolute log2FC 1, A).', 'After achieving an effective knock-down confirmed by RT-qPCR (B), cells were challenged in terms of proliferation, reproductive capacity, adhesion (C E), and in vivo growth (F H), obtaining the same results that in the case of ERK5 abrogation or even more striking differences.', 'KLF2 is a key effector of the ERK5 biological properties in 3MC-C1 cell line.']","Figure 6 ( ) Volcano plot showing transcription factors affected by ERK5 abrogation. Volcano plot showing the effect size of ERK5 attenuation on gene expression (log2 fold change in ERK5-depleted over control cells) versus statistical significance of the difference in expression between conditions (log10 of the FDR-adjusted -values). Each dot represents an independent gene. DEG as determined by statistical significance (FDR < 0.05), effect size (|Log2FC| > 0.99) or both are shown in blue, green, and red colors respectively. Text labels show the identity and location of the transcription factors significantly affected (FDR < 0.05 and |Log2FC| > 0.99) by ERK5. ( ) RT-qPCR showing effective knock-down of expression in 3MC-C1 cells infected with lentiviral vectors coding for empty vector (E.V.) or an shRNA specific for (shKLF2), using -2-microglobulin ( ) as an endogenous control. E.V. cells were refereed as 1. Histogram shows the average of 3 independent pools of infection. ( ) For growth curves, 3 10 E.V. or shKLF2 3MC-C1 cells were seeded in 100 mm plates. Every 3 days, cells were counted and replated in the same manner up to day 9. Graphic shows the cumulative cell number from a representative experiment out of 3 with nearly identical results in different pools of infections. ( ) Upper panel: Relative number of colonies obtained in clonogenic assays of E.V. or shKLF2 3MC-C1 cells. Colonies formed by E.V. cells were considered as 1. Lower panel: Representative image of a colony formation assay at 7.5 and 60 min. ( ) Upper panel: Relative adhesion of E.V. or shKLF2 3MC-C1 cells was assessed by crystal violet staining at different time points. Graphic shows mean of 3 independent experiments performed in triplicated cultures with 3 different pools of infection. Lower panel: Representative image of adhesion assays at 7.5 and 60 min. ( ) Nude mice (n = 4) were inoculated with 5 10 E.V. and shKLF2 3MC-C1 cells, and tumor growth was analyzed at the indicated times. Final tumor weight for E.V.-derived tumors was 0.30 g 0.05 and for shLKF2-derived tumors was 0.29 0.09. The experiment was performed by using another pool of infections with nearly identical results. ( ) Representative images of the histological study of tumors obtained from E.V. and shKLF2 3MC-C1 cells. Pictures are depicted at a 20 magnification. ( ) RNA from tumors induced by E.V. and shKLF2 3MC-C1 cells was extracted, and expression levels were measured by RT-qPCR in triplicate using as an endogenous control. The graphs/histograms represent the mean SD of 3 independent experiments performed in triplicate cultures from different pools of infections, if not otherwise indicated. *** < 0.001, **** < 0.0001.",yes
PMC11508542,Figure_4,oa_package/1a/5f/PMC11508542.tar.gz,"[': Newark, DE, USA) is an aortic endograft with a single side branch for the LSA, designed to treat aneurysms/dissections requiring a zone 2 TEVAR ().', '(A) Gore TAG Thoracic Branched Endograft, (B) TBE within aneurysm.']",Figure 7 ( ) Zenith arch branched device. ( ) Internal side branches. ( ) External view of side branches along outer curve of graft. Courtesy of Cook Medical [ ].,yes
PMC9454751,Figure_3,oa_package/55/d2/PMC9454751.tar.gz,"['Claudin-4 Expression PatternsClaudin expression levels vary with tumor types, tumor aggression, and invasion potential [13] ().', 'Claudin expression patterns can also indicate tumor type as the distinct fashion in which the Claudin proteins aggregate in cells depends on the cell type and location [27,28,29,30,31,32,33,34,35,36,37,38,39,40,41,42,43,44] ().', 'Reconstructed heatmap with graph prism showing Claudin protein expressions as biomarkers for cellular differentiation and indicators for phenotypic expression of the epithelia from several research studies [26,27,28,29,30,31,32,33,34,35,36,37,38,39,40,41].']","Figure 3 Reconstructed heatmap with graph prism showing Claudin protein expressions as biomarkers for cellular differentiation and indicators for phenotypic expression of the epithelia from several research studies [ , , , , , , , , , , , , , , , ]. Claudin protein expression patterns in varying cancer types can therefore provide information for cancer identification.",yes
PMC10550547,Figure_3,oa_package/46/fc/PMC10550547.tar.gz,['Onyx was delivered slowly to prevent uncontrolled extravasation to surrounding tissue outside the hemangioma ().'],"FIG. 3. Anteroposterior intraoperative fluoroscopy demonstrates the right-sided intraosseous hemangioma ( ), with the late arterial phase demonstrating the full extent of vertebral body involvement ( ). The segmental artery is seen lateral ( ) and anterior ( ) to the vertebral body, marked by black arrows. Repeat angiography after Onyx embolization ( ) demonstrates significantly reduced contrast opacification of the vertebral body and no compromise of the segmental artery ( ). The Onyx cast at the end of embolization is seen in anteroposterior ( ) and lateral ( ) views.",yes
PMC6166693,Figure_2,oa_package/02/a9/PMC6166693.tar.gz,[],"FIGURE 1 Theoretical framework behind the use of a negative addition lens in tilted disc syndrome. The yellow dashed lines indicate a circular arc. In (A), a patient with tilted disc syndrome has posterior bowing of the retina (blue arrow). A negative addition lens can focus rays of light more appropriately at the region of posterior retinal bowing by diverging rays of light. A patient with generally regular retinal curvature is shown in (B). The green arrows indicate regions with near normal curvature and/or slight anterior shift (relative hyperopic defocus). The fundus photographs of the corresponding representative patients are shown directly below. In (C), the optic nerve head displays tilt and torsion, with situs inversus of the adjacent retinal blood vessels typical of tilted disc syndrome. In (D), the optic nerve head is fairly oval in appearance, without tilt or torsion.",yes
PMC5922330,Figure_1,oa_package/4e/3b/PMC5922330.tar.gz,"['Features on CXRs are a non-segmental, homogenous consolidation predominantly involving one lobe with air bronchograms (large bronchi remain patent and air-filled in contrast to the adjacent non-aerated lung) [25] ().', 'Chest radiograph showing right lower lobe pneumoniaBronchopneumonia is thought to be usually associated with infections due to gram-negative bacteria, Staphylococcus aureus and some fungi [25].']",Figure 1 Chest radiograph showing right lower lobe pneumonia,yes
PMC9563334,Figure_2,oa_package/7e/ff/PMC9563334.tar.gz,[],"Figure2 Spatial transcriptomics and single cell-based analysis of human myocardial B-cells. Distribution of B-cells, and T-cells in myocardial tissue analyzed with 10X Visium spatial transcriptomics technology. Blue dots represent tissue regions where expression of the B-cell marker is identified. Green dots represent tissue regions where expression of T-cell gene expression marker is identified. UMAP plot of single-cell gene expression analysis of myocardial immune cells from the human Heart CEll Atlas. B-cells are highlighted in blue and T-cells are highlighted in red. B-cells represent 3% of all immune cells (1,196 of 40,868) while T-cells represent 24% of the same population (10,297 of 40,868). ImmGen MyGeneSet analysis of the genes with the highest differential expression between the larger B-cell cluster and the rest of the immune cells analyzed. The analysis suggests that this B-cell population is most similar to T1 (transitional) B-cells. ImmGen MyGeneSet analysis of the genes with the highest differential expression between the smaller B-cell cluster and the rest of the immune cells analyzed. The analysis suggests that this B-cell population is most similar to plasma cells.",yes
PMC2842892,Figure_6,oa_package/3b/f2/PMC2842892.tar.gz,"['5 m ().', '005""/>Endoscopy of UH-OCT.']","Figure 6 Endoscopy of UH-OCT. The upper image is the OB and the lower image shows the histology of the area. (Taken from P. Hsiung, L. Pantanowitz, et al. (2005) .)",yes
PMC11458539,Figure_2,oa_package/59/74/PMC11458539.tar.gz,"['At the early stage, P301S mice exhibited limited tau hyperphosphorylation, and Nlrp3 deletion did not affect tau phosphorylation (AT100) in the spinal cord, brainstem, midbrain, and cortex (s 2A,B).', 'A significant reduction in phosphorylated tau levels was detected only in the spinal cord of Nlrp3 / xP301S mice compared to Nlrp3+/+xP301S mice (s 2A,B).', 'No effect of Nlrp3 knockout on tau pathology by IHC.', ', 2018) - were unaffected by Nlrp3 knockout in P301S mice at both early and late stages (C).', 'In the total homogenate, aggregated tau levels (analyzed by the AT8/AT8 MSD assay) increased at the late stage of pathology, but Nlrp3 deficiency did not influence tau pathology (C).', 'Gsdmd deletion was evaluated and confirmed in vitro using Western blotting and cytokine analysis (Supplementary ).']","Figure 2 No effect of knockout on tau pathology by IHC. AT100 immunohistochemistry demonstrated no significant effect on tau phosphorylation/aggregation in all analyzed regions of late-stage xP301S vs. xP301S mice. Brain stem, spinal cord, and midbrain showed a trend in reduced pathology for deficiency, which was not significant. Early stage P301S mice displayed much lower aggregated tau levels compared to late-stage animals and no effect of deletion on tauopathy was observed at this age in any of the regions analyzed. Image analysis data are shown as mean+individual values for each animal (average of two sections/animal). Early stage xP301S: =24, xP301S: =26; Late-stage xP301S: =18, xP301S: =19. No significance by unpaired -test of age-matched groups. Representative images of the AT00 IHC staining are demonstrated. Scale bars: 2.5mm (overview images), 100m (higher magnification images). Biochemical analysis of tau pathology in brainstem of xP301S mice. Aggregated tau levels were not significantly reduced in the sarcosyl-insoluble fraction or total homogenate of the brainstem of late-stage vs. xP301S mice. Early stage xP301S: =23, xP301S: =25; Late-stage xP301S: =16, xP301S: =16. Data are expressed as mean+individual values of each animal tested (values=average of two sections/mouse).",yes
PMC5666312,Figure_5,oa_package/f9/ad/PMC5666312.tar.gz,['\ncDC1 are required for the development of parasite specific IFN +\nTNF\n+ Th1 responses during PbA blood stage malaria\nExperimental protocol to analyze T cell responses in Karma mice at day 6 post infection with PbA pRBC.'],Figure 3 Immunodominance of Aderived peptides during bloodstage malaria induced after spz infection,yes
PMC5617844,Figure_3,oa_package/e6/43/PMC5617844.tar.gz,"[' 3a).', 'C1-Ten PTPase activates mTORC1.', '\nBecause nephrin dephosphorylation is responsible for mTORC1 activation, and because C1-Ten functions as a PTPase for nephrin, we investigated whether the PTPase activity of C1-Ten activates mTORC1.', ' 3b), but expression of the C1-Ten CS mutant did not increase S6K1 phosphorylation (', ' 3b); this result is consistent with the hypothesis that C1-Ten PTPase activity regulates mTORC1.', ' 3c).', ' 3a).', ' 3b).']","Figure 3 C1-Ten PTPase activates mTORC1. ( ) Effect of nephrin Y1138F mutant on IRS-1-mediated mTORC1 activation. HEK293 cells were transfected with GFP vector, GFP nephrin WT, or GFP nephrin Y1138F for 24 h. The cells were serum-starved for 18 h, then treated with 10 nM insulin for 30 min. mTORC1 activation was measured by immunoblotting of phospho- and total- S6K1. Data are meansSEM (n=3). ( ) Effect of C1-Ten overexpression on mTORC1 activation. Human podocytes were transduced with Adenovirus (Ad) GFP, Ad C1-Ten WT or Ad C1-Ten CS for 48 h. Protein expression of phospho- and total-S6K1 were detected by Western blotting. Data are meansSEM (n=3). ( ) Effect of C1-Ten knockdown on the HG-mediated mTORC1 activation. mTORC1 activation was presented by immunoblotting of phospho- and total- S6K1. Data are meansSEM (n=3). *P <0.05.",yes
PMC8634485,Figure_3,oa_package/8e/54/PMC8634485.tar.gz,"['Magnetic resonance imaging (MRI) revealed a well-defined lesion with low signal intensity on T1 and high signal intensity on T2 weighted images ().', '\n\nCase 1, (A): Axial T2-weighted MR image of twelfth thoracic spine vertebrae; (B): Sagittal T2-weightedimages of thoracic spine vertebrae.']","Figure 3 Case 1, ( ): Axial T2-weighted MR image of twelfth thoracic spine vertebrae; ( ): Sagittal T2-weightedimages of thoracic spine vertebrae.",yes
PMC3065475,Figure_5,oa_package/f2/b2/PMC3065475.tar.gz,"['The data presented in refer to 15 patients and 53 treatments with dexamethasone or ACTH.', 'g005Efficacy of dexamethasone and ACTH in drug resistant pediatric epilepsy.', 'g005""/>\nA shows the efficacy of dexamethasone or ACTH treatments.', 'The mosaic plot in B shows the distribution of the overall efficacies (set as 50 ) across different etiologies.']",10.1371/journal.pone.0018200.g005,yes
PMC4059651,Figure_4,oa_package/20/52/PMC4059651.tar.gz,"['Pre-treatment of CASMCs with TNF or IFN (50 ng/mL) for 12, 24 and 36 h significantly increased mDCs adhesion to CASMCs (A).', 'The DCs adhering to CASMCs contacted the latter cells through their dendrites, with small areas of close approach ( 20 nm) between membranes (B and C).', 'g004Adhesion of mDC to vascular smooth muscle cells.', ' 7, 14 and 21 days after injury (D-G).', '01 1 mol/L) or (B) rosiglitazone (1 20 mol/L) before stimulation with 50 ng/mL TNF or IFN for 24 h followed by assay of DC adhesion as indicated for .']",10.1371/journal.pone.0099652.g004,yes
PMC4672422,Figure_2,oa_package/0a/5e/PMC4672422.tar.gz,"['Cerebral and cerebellar meninges were moderately expanded with lymphocytes, plasma cells, and macrophages (), and cerebral vasculitis was associated with surrounding malacia.', 'Cerebellum of dog infected with Hendra virus, showing expansion of the meninges with inflammatory infiltrates (*) and marked vasculitis (arrow).']","Figure 2 Cerebellum of dog infected with Hendra virus, showing expansion of the meninges with inflammatory infiltrates (*) and marked vasculitis (arrow). Scale bar indicates 75 m.",yes
PMC6251292,Figure_2,oa_package/6a/8d/PMC6251292.tar.gz,['Presence or absence of accessory mandibular foramen was also recorded [].'],Figure 2 Accessory mandibular foramen,yes
PMC8492323,Figure_2,oa_package/4b/c3/PMC8492323.tar.gz,"['To examine potential regulation of MLK3 by PKG1 , we first tested whether PKG1 and MLK3 interact in LV tissue, and we observed coimmunoprecipitation of MLK3 with PKG1 in WT mouse LV tissue lysates (A).', 'This interaction requires the PKG1 LZ domain, as the GST-PKG1 LZ domain was sufficient to pull down MLK3, whereas GST-PKG1 mutant LZ domain (16) or GST alone failed to precipitate MLK3 (B).', 'MLK3 coprecipitated with WT, but not LZM PKG1 , indicating both direct cointeraction of MLK3 and PKG1 , as well as a requirement of the PKG1 LZ domain for this interaction (C).', 'We observed a 32% 11% reduction of PKG1 precipitation, normalized to MLK3 in LV tissue from WT mice subjected to LV pressure overload by TAC compared with sham surgery (D).', 'Bioinformatics analysis using NetPhosK software (19) revealed a potential PKG1 target site at Ser281 within the kinase domain of human MLK3 (E).', 'In HEK293 cells transfected with PKG1 and with FLAG-tagged MLK3 (20), the administration of 8-Bromo-cGMP, a membrane permeable PKG activator, induced MLK3 phosphorylation on the Thr277/Ser281 kinase activation site (F).', 'In vitro coincubation of purified PKG1 with MLK3 also induced MLK3 phosphorylation on these sites, supporting that MLK3 was a direct substrate of PKG1 (G).', 'Activity level and heart rate did not differ between genotypes (Supplemental ).', 'PKG1 interacts with and phosphorylates MLK3.']","Figure 2 PKG1 interacts with and phosphorylates MLK3. ( ) Coimmunoprecipitation of MLK3 and PKG1 from mouse LV lysate (representative image from = 5). ( ) Coprecipitation of the GST-tagged N-terminal PKG1 LZ domain (amino acids 159), and PKG1 amino acids 1 to 236, with endogenous MLK3 from mouse LV lysate. Coomassie staining confirmed protein input of GST-tagged proteins (from = 3 separate experiments). Arrows denote GST fusion proteins. ( ) Full-length PKG1 WT or LZM were affinity purified and incubated with recombinant MLK3. Coprecipitation of recombinant MLK3 detected by immunoblotting (representative of = 4). ( ) Disruption of PKG1-MLK3 cointeraction in the pressure overloaded LV. Immunoprecipitation of MLK3 in left ventricular lysate from mice subjected to sham vs. 7-day transaortic constriction. Representative IB for PKG1 or MLK3. Graph of PKG1 normalized to MLK3 in immunoprecipitant. = 4 independent experiments. * < 0.05. ( ) Analysis of PKG1 target sites on MLK3 using NetPhosK 1.0 reveals potential phosphorylation site Ser281 within the MLK3 kinase domain. ( ) HEK293 cells transfected with FLAG-MLK3 were stimulated with 8-Bromo-cGMP (8-Br-cGMP, 5 mM, 30 minutes) or Veh. FLAG immunoprecipitant from lysates immunoblotted for P-MLK3 on residues Thr277/Ser281 ( = 3). ( ) Phosphorylation of recombinant MLK3 on residues Thr277/Ser281 assessed in kinase reaction mixture containing recombinant MLK3 and purified PKG1 proteins ( = 3). For all experiments each replicate is an independent experiment. * < 0.05 by Students unpaired 2-tailed test. PKG1, cGMP-dependent protein kinase 1; MLK3, mixed lineage kinase 3; LV, left ventricle; GST, glutathione- -transferase; LZ, leucine zipper; LZM, leucine zipper mutant; Veh, vehicle; ADU, arbitrary densitometric units ; P-MLK3, phosphorylation of MLK3; P/T: Ratio of phosphorylated to total; IB, immunoblot.",yes
PMC2995998,Figure_4,oa_package/24/09/PMC2995998.tar.gz,"['Fibrovascular stroma with nests and clusters of cells arranged in an alveolar pattern (H and E, 4)Central part of the alveoli made up of small and round cells with little cytoplasm and dark nuclei whereas the peripheral part comprised of cuboidal, flattened epitheloid cells (H and E, 4)Peripheral melanin-containing epitheloid cells showing positivity (Masson Fontana, 4)IHC staining showing HMB-45 positivity in peripheral epitheloid cellsIHC staining showing synaptophysin positivity in central small neuroblast-like cells ( 10)The patient s recovery was uneventful and healing progressed satisfactorily.']","Figure 4 Fibrovascular stroma with nests and clusters of cells arranged in an alveolar pattern (H and E, 4)",yes
PMC10619506,Figure_1,oa_package/fc/f8/PMC10619506.tar.gz,"['There was enrichment for RAR target genes in bulk and PTEC RNA-Seq data sets (Table 1; , A and B; and Supplemental Table 2), while thick ascending limb (TAL) and renal leukocyte populations showed less robust enrichment for RAR target genes (Table 1 and Supplemental Table 2).', 'More detailed analysis of PTEC subsets indicates that RAR target genes are predominantly activated in injured, repairing, and failed repair PTECs (which also express KIM1) but also in differentiated S3 segment PTECs, which are found in the outer stripe of the outer medulla, in AKI samples (Supplemental ).', 'Principal component analysis (PCA) of these data sets shows clear separation of the 2 genotypes along the first dimension, accounting for 57% of the variation between samples (Supplemental 2).', 'Gating was then set to distinguish between F4/80 (early infiltrating monocytes), F4/80int (BM-derived renal macrophages), and F4/80hi (kidney-resident macrophages [KRM]) cells, as described (Supplemental 3) (67, 69).', 'There was an expected increase in total CD45+, CD11B+, and CD45+CD11B+ nongranulocyte (ly6G ) mononuclear cells after IRI-AKI, there was no difference in total cell numbers between PEPCK Cre+ and Cre kidneys (Supplemental 4).', 'As previously reported (67), we also detected high levels of MHC-II expression in F4/80hi cells from uninjured mice, and there were fewer F4/80hi MHC-IIhi cells after injury (Supplemental 5, A and B).', 'There was a significant increase in EdU+F4/80hi cells in PTEC DN RAR compared with Cre controls after AKI (Supplemental 5, C and D).', '71) in PTEC DN RAR mice after IRI-AKI (Supplemental 5, G I).', '--7132317-->31411074Version 109/12/2023In-Press PreviewVersion 210/23/2023Electronic publicationActivation of retinoic acid receptor (RAR) signaling in patients with sepsis-associated AKI.']",Figure 1 Activation of retinoic acid receptor (RAR) signaling in patients with sepsis-associated AKI. Gene set enrichment analysis (GSEA) of bulk RNA as well as cell-specific snRNA-Seq kidney data sets from patients with SA-AKI compared with age-matched controls from Hinze et al. ( ). ( and ) Volcano plots indicating fold change in expression of core enrichment genes (AKI versus controls) from bulk RNA and PTEC snRNA-Seq data sets. Dotted line indicates < 0.05.,yes
PMC3596751,Figure_1,oa_package/b5/fb/PMC3596751.tar.gz,"['(shown in )', '005, ).']",Fig. 1 Expressions of lysyl oxidase-like 1 (A) and fibulin-5 (B) by immunohistochemistry (magnification: 400; scale bar: 50 m). Positive expression was indicated by brown-yellow granules in the cardinal ligament tissues of patients with pelvic organ prolapse.,yes
PMC6739147,Figure_7,oa_package/6b/a7/PMC6739147.tar.gz,[],,yes
PMC8387632,Figure_2,oa_package/84/d9/PMC8387632.tar.gz,"['In particular, patients with stage 2 hypertension have the highest cumulative incidence of CVD mortality and stroke, followed by stage 1 hypertension, elevated BP, and normal BP ().', 'The cumulative incidence of adverse events by the different BP groups.']","Figure 2 The cumulative incidence of adverse events by the different BP groups. Incident All-Cause Mortality, Cardiovascular Disease Mortality, Stroke Incidence, and Myocardial Infarction. Error bars represent 95% CI. Normal: SBP <120 mm Hg and DBP <80 mm Hg; Elevated: SBP of 120129 mm Hg and DBP of <80 mmHg; Stage 1: SBP of 130139 mmHg or DBP 8089 mmHg; Stage 2: SBP/DBP 140/90 mm Hg, or taking antihypertensive medications. SBP, systolic blood pressure; DBP, diastolic blood pressure. CI, confidence interval. y, year; Stage 1, Stage 1 hypertension; Stage 2, Stage 2 hypertension.",yes
PMC5336777,Figure_3,oa_package/a1/03/PMC5336777.tar.gz,"['Statistical analysis was used: one-way ANOVA and posthoc Tukey testApoptosis (TUNEL) findingsTUNEL staining was performed to determine apoptotic cells in heart tissue ().', 'a-f.']","Figure 3 a-f. TUNEL staining of heart tissue in different groups: (a) group C, (b) group D, (c) group D+PI, (d) group D+PII, (e) group P, and (f) negative controls. TUNEL-positive cells (arrow) were observed in the heart. (Statistical analysis was used One-way ANOVA, posthoc Tukey test)",yes
PMC10777279,Figure_5,oa_package/f9/bf/PMC10777279.tar.gz,"[' shows examples of inconsistencies observed across the crowds.', 'a shows consistent demarcation of highly affected areas by both the high and low top 5 crowds; however, there was poor identification of subtle surface changes, which were also marked in the expert ground truth.', 'b demonstrates an instance where the high feedback crowd failed to identify abnormal changes while the low top 5 crowd identified 75% of the abnormal skin area.', 'c highlights an instance of high variability between images from different angles of same skin region in the low top 5 crowd, where 2 images show good agreement with the ground truth, but the third image consensus predicted no affected skin.', 'Reliability of DemarcationsDespite good median performance of the different crowds across the full set, we observed a number of high error images in all groups (b).']","Figure 5 Example demarcations from the crowd (blue) versus ground truth (green). (A) Consistent demarcation of highly affected areas by crowds assembled from both the high and low feedback groups, but both missed areas of subtle surface changes. (B) The high feedback crowd failed to identify abnormal changes while low feedback top 5 crowd identified 75% of abnormal skin areas. (C) High variability between images of the same skin region viewed from different angles by the low feedback top 5 crowd. cGVHD: chronic graft-versus-host disease; r: rater.",yes
PMC6686509,Figure_1,oa_package/98/68/PMC6686509.tar.gz,"[' 1).', '', 'The formation of neoantigens enhanced immune cell recognition and the effectiveness of immunotherapy\nIt is however important to note that TMB alone does not represent a direct evidence of tumor immunogenicity, due of the complex dynamics that underlie the host immune response in the context of tumor cells and their microenvironment.']",Fig.1 Schematic diagram of tumor cell with high TMB and its relationship with the immune system. The formation of neoantigens enhanced immune cell recognition and the effectiveness of immunotherapy,yes
PMC7240057,Figure_1,oa_package/b6/5b/PMC7240057.tar.gz,"['Conventional bilateral inguinal node lymphangiography was performed with ethiodized oil, revealing bilateral foci of retroperitoneal extravasation at L3-L4 ().', 'Frontal fluoroscopic image following bilateral inguinal access and lymphangiography using ethiodized oil (A) demonstrated two foci of lymphatic extravasation in the retroperitoneum (arrows).', ' The patient underwent a subsequent paracentesis 3 days later with removal of 3 L fluid.']","Fig. 1 Frontal fluoroscopic image following bilateral inguinal access and lymphangiography using ethiodized oil (A) demonstrated two foci of lymphatic extravasation in the retroperitoneum (arrows). Following prone positioning, foci of extravasation were targeted using cone beam CT guidance (B). Lymphatic fluid draining from the access needles was noted (C). Each site was embolized using cyanoacrylate (D) (arrowheads).",yes
PMC9549086,Figure_2,oa_package/58/aa/PMC9549086.tar.gz,"['Biopsy forceps were used to clamp the lesion site , and lung tissue\nwas cut as far as possible and retracted strongly.', '1177_14799731221133389-fig2"" position=""float""/>Procedure, numbers, and biopsy sitesSurgery was completed in all cases, and patients were safely returned to the\nwards post-operatively.']",Figure2. Biopsy forceps were used to clamp the lesionsite.,yes
PMC9595131,Figure_4,oa_package/7a/28/PMC9595131.tar.gz,"['A statistically significant increase in the levels of the proinflammatory cytokine IL-6 and chemokine CXCL1 was detected in microglia treated with 40 nM of MMP10, with CXCL1 being released in a dose responsive manner (A).', 'TNF was also observed in microglia upon stimulation with both 20 and 40 nM cMMP10 compared to vehicle-control cells (B).', 'Moreover, the release of TNF was reversed when cMMP10 was incubated with GM6001, as TNF levels in this group were not significantly compared to VEH (B).', 'In contrast, unlike LPS-induced IL-1 and IL-4 release, stimulation of microglia with either dose of cMMP10 did not result in the secretion of IL-1 or IL-4 secretion (C).']","FIGURE 4 The release of classical proinflammatory cytokines is increased in cMMP10-stimulated microglia. Microglia were exposed to cMMP10 (20 or 40 nM) or vehicle (VEH) for 24 h. Cells stimulated with 40 nM cMMP10, but not 20 nM cMMP10, significantly increase IL-6. These cells also release CXCL1 release in a dose responsive manner to cMMP10. cMMP10-exposed microglia demonstrate higher levels of TNF in a dose responsive manner compared to VEH treated cells (ELISA; = 4 wells/treatment). The increase of TNF following cMMP10 exposure was reversed when cMMP10 was incubated with the pan-MMP10 inhibitor GM6001 (ELISA; = 3 wells/treatment). cMMP10 is not sufficient to stimulate the release of IL-1 or IL-4. Values reported are representative of one experiment performed with three technical replicates, or wells, per treatment. The experiment was conducted independently two additional times. Values are reported as mean SEM; One-way ANOVA compared to VEH; Dunnetts test; 0.0001, 0.001, 0.01, and * 0.05.",yes
PMC6235468,Figure_2,oa_package/4b/34/PMC6235468.tar.gz,[],"Figure 2 Histological findings of colorectal liver metastasis. Partial pathologic response with necrosis; pathologic response evaluation of colorectal liver metastases according to Rubbia-Brandt is classified as TRG4 and is evaluated as a minor response (more than 50% of residual tumor cells) according to Blazer classification (hematoxylin and eosin, magnification 200)",yes
PMC9572649,Figure_3,oa_package/f4/62/PMC9572649.tar.gz,"['We analyzed the area of A localization via the area of Nisin staining, and there was no A aggregation in the brains of the WTC and WTE groups, while the ADC and ADE groups showed A aggregation with blue-black spots (A).', 'As can be seen in B, the hippocampal niosomes are densely arranged in each group of mice, and the niosomes in the cerebral cortex of the WTC, WTE, and ADE groups are more darkly stained and densely arranged, while the niosomes in the ADC group are more lightly stained, cavernous, and sparsely arranged, which is consistent with recently reported results [30].', 'Exercise alleviates A pathology and neuronal loss in mice.']","Figure 3 Exercise alleviates A pathology and neuronal loss in mice. ( ) Representative images of A immunohistochemistry. The black arrows point to A plaques. ( ) Representative images of Nissl staining. The neuron Nissl body is shown in blue. ( ) Histogram showing the percentage of A-stained area. ( , ) Histograms showing the percentage of Nissl-staining area in the ( ) hippocampus and ( ) cerebral cortex ( = 4 per group). Data are presented as means SDs. # < 0.05 vs. WTC mice; * < 0.05, and ** < 0.01 vs. ADC mice.",yes
PMC6992992,Figure_5,oa_package/35/31/PMC6992992.tar.gz,"['4Microtubule organization 5 shows disarray of the tubulin network in mutant LMNA NRVMs compared to controls.', 'To quantify the tubulin network organization in the different mutations ( 5A), we used the optical Haralick correlation metric.', 'As shown in Table 1 and 5B, the three mutant LMNA cell models showed contrast, uniformity (homogeneity or angular second moment), local homogeneity (Inverse Difference Moment (IDM)), entropy and correlation significantly different from the control LMNA models, indicating less organized microtubule networks with shorter microtubule filaments length.', ' 5Microtubule network organization.', 'Trafficking of Cx43 is regulated in part by the microtubule network [38, 39] and in fact, we observed a partial variation of the microtubule organization compared to our control cells as indicated by the disarray of the microtubule network ( 5, Table 1), with less organized microtubule networks and shorter microtubule filaments length.']","Figure5 Microtubule network organization. (A) Example of microtubule organization used to calculate the Haralick features. (B) Haralick texture features: Contrast, Angular second moment (energy), Inverse difference moment (homogeneity), Entropy and Correlation are shown only for an angle of O (complete set of data in ); Data are presented as mean S.D. ( = 4 independent experiments). * < 0.05; ** < 0.005; *** < 0.0001.",yes
PMC4742118,Figure_6,oa_package/b8/5d/PMC4742118.tar.gz,['Calcium-dependent binding of 1Rs to MORs and NR1 subunits: Influence of 1R regulationa.'],"Figure 6 Calcium-dependent binding of 1Rs to MORs and NR1 subunits: Influence of 1R regulation . The recombinant MOR, NR1 C0-C1-C2 and 1R were used at 100 nM. The assay was performed in the presence of increasing amounts of calcium chloride (0, 0.25, 0.75, 2.5 mM). Bait proteins (GST-NR1 C0-C1-C2 and GST-MOR) were immobilized by covalent attachment to NHS-activated Sepharose. Prey proteins alone did not bind either to the NHS-Sepharose or to the recombinant GST (negative controls). The pellets obtained were processed as described to determine 1Rs in Western blots (see the Methods section). The bars are the mean S.E.M of three independent assays. Effect of calcium. For each interaction of 1R, MOR-1R and NR1-1R, the effects of increasing calcium availability are shown relative to the data obtained in the absence of calcium control group (C): arbitrary value of 1): *Significant differences, ANOVA (DF = 11), Dunnett multiple comparisons control group, < 0.05. Effects of 1R agonism (bars in red). These effects are indicated for each interaction of 1R and calcium concentration studied: significant difference between the paired groups at each calcium concentration studied, with and without the sigma ligand; ANOVA (DF = 23) all pairwise Holm-Sidak multiple comparison test, < 0.05. While diminishing 1R binding to NR1 subunits, Progesterone, a 1R antagonist, has a calcium-dependent effect at 1R-MOR complexes. Details as in (a). Effect of exogenous (synthetic) ligands of 1Rs on the NR1-1R complex: agonists, PRE084 and SKF10047; antagonists, BD1047, BD1063 and NE100. *Significantly different from the control group that received saline instead of the 1R ligand; ANOVA (total DF = 11), Dunnett multiple comparisons control group, < 0.05.",yes
PMC2820081,Figure_7,oa_package/cd/da/PMC2820081.tar.gz,"['One, the low expresser, exhibited a faint, while the other, the high expresser, showed a strong, green fluorescence after Dox induction (A).', 'Western blotting also confirmed that the induced level of optineurin-GFP was low in the former but high in the latter (B).', 'g007Inducible expression of optineurin-GFP in Tet-on RGC5 stable cell lines.', 'In the low expressers, optineurin-GFP had a diffuse, cytosolic distribution (), comparable to that seen in the endogenous situation.', 'In the high expressers, the foci were formed in the perinuclear region proximal to the Golgi (), similar to that observed in pOPTN-EGFP-transfected cells (']",10.1371/journal.pone.0009168.g007,yes
PMC8491700,Figure_2,oa_package/3e/8e/PMC8491700.tar.gz,"['Cellular viability and uptake studiesThe cytocompatibility of HA/TA-LP was also a crucial indicator for potential applications, which was evaluated using MTT ((A)).', '.', 'Examination of the fluorescence profiles of cells indicated their intensity gradually increased with incubation time, indicating that TA was delivered into cells ((B)).', 'Furthermore, the targeting delivery of HA/TA-LP was further investigated using TEM ((C)).', 'Toward this, A375 cells were treated with HA/TA-LP or HA-TA before measuring cellular tyrosinase activity and melanin content ((D)).']","Figure 1. (A, B) TEM and schematic images of TA-LP and HA/TA-LP nanogels, (C, D) Cryo-TEM and SEM images of HA/TA-LP, and (E, F) the diameter distributions and TA release profiles of HA/TA-LP and HA-TA.",yes
PMC5436327,Figure_1,oa_package/1a/2f/PMC5436327.tar.gz,"['(a) Whole body planar imaging at 24 h postinjection show a readily visible focal lesion in the right neck in the anterior projection (arrow labelled MTC = Medullary thyroid carcinoma) and (b) bilateral lesions in the region of the adrenal glands on the posterior projection (arrow labelled PC = Pheochromocytoma)Reformatted single-photon emission computed tomography imaging at 24 h postinjection show: A focal unequivocally iodine-123 meta-iodobenzylguanidine avid lesion in the region of the right thyroid on coronal (a) and transaxial (c) images, and unequivocally iodine-123 meta-iodobenzylguanidine avid lesions in the bilateral adrenal glands on coronal (b) and transaxial (d) imagesThe patient subsequently underwent successful bilateral robotic adrenalectomy.']",Figure 1 (a) Whole body planar imaging at 24 h postinjection show a readily visible focal lesion in the right neck in the anterior projection (arrow labelled MTC = Medullary thyroid carcinoma) and (b) bilateral lesions in the region of the adrenal glands on the posterior projection (arrow labelled PC = Pheochromocytoma),yes
PMC6678254,Figure_3,oa_package/bd/02/PMC6678254.tar.gz,"['Detection of BK Receptors and KCa ChannelsWestern blot analyses of BK receptor expression in homogenates from third order mesenteric arteries from SHR and Wistar rats revealed immune-reactive bands of ~36 kDa for the type 1 BK receptor (B1R) and ~45 kDa for the type 2 BK receptor (B2R) that were comparable to protein bands detected in homogenates prepared from human intra-thoracic arteries and human umbilical vein endothelial cells expressing native BK receptors (A,B).', 'SHR mesenteric arteries exhibited reduced B1R and B2R expression compared with arteries from Wistar rats (C).', '1 channel (~45 kDa band) proteins in mesenteric arterial homogenates from the two strains (D F).', '1 channels were decreased 2-fold, as revealed by qPCR analyses (G).', '1 channel expression was comparable ().', '1 channel protein and mRNA observed in small mesenteric arteries from Wistar rats and SHRs ().', 'Molecular detection of bradykinin (BK) receptors and KCa channels in Wistar and SHR mesenteric arteries.']","Figure 3 Molecular detection of bradykinin (BK) receptors and KCa channels in Wistar and SHR mesenteric arteries. The protein expression of BK receptor type 1 (B1R, panel ( )) and type 2 (B2R, panel ( ), along with KCa2.3 and KCa3.1 channels (panels ( and ( ) was analyzed by western blot in mesenteric artery homogenates derived from age-matched SHRs and Wistar rats. Homogenates prepared from human intra-thoracic arteries (ITA) and human umbilical vein endothelial cells (HUVECs) served as positive controls for the detection of B1R and B2R proteins. Lysates prepared from HEK 293 cells transfected with cDNA encoding either KCa3.1 or KCa 2.3 channels were used as positive controls and are displayed in lane 1 of the corresponding blots. HEK 293 cells transfected with cDNA encoding mouse Kir2.1 channel served as a negative control. Quantification of protein expression for B1R and B2R, and KCa2.3 and KCa3.1 channels is shown in panels ( and ( , respectively. Staining intensities of selected immuno-reactive bands are expressed as a ratio with detected -actin expression in the same homogenate. The asterisk (*) signifies a statistical difference between the indicated groups ( = 45 animals), as determined by a Students t-test, < 0.05. Panel ( quantifies the levels of mRNA encoding B1R, B2R, KCa2.3 channel, and KCa3.1 channel in mesenteric arteries from SHRs and Wistar rats, as determined by real-time, quantitative PCR analyses. The ratio of mRNA expression for each target in the Wistar and SHR mesenteric arteries was calculated using REST software [ ]. Detection of GAPDH mRNA was utilized as an internal reference in all assays. Data are expressed as means S.D. and statistical analyses were determined by one-way ANOVA and a Tukeys post-hoc test ( = 45 animals per group).",yes
PMC5732350,Figure_3,oa_package/08/0b/PMC5732350.tar.gz,[],"Figure-3 Lung section showed the presence of peribronchiolar and perivascular lymphoid follicular aggregates (arrow) with infiltration of lymphocytes (inset) into the interalveolar spaces (H and E, 100).",yes
PMC2719206,Figure_6,oa_package/5b/97/PMC2719206.tar.gz,"['096) ().', 'The correlation between immunohistochemical Nogo-B activity, and plaque composition and CD68/MMP-9 expression.']","Fig. 6 The correlation between immunohistochemical Nogo-B activity, and plaque composition and CD68/MMP-9 expression. Nogo-B expression carried a significant negative correlation with core necrosis ( ), but not with calcification ( ). Nogo-B expression had a significant negative correlation with the number of CD68-positive cells ( ), but not with MMP-9 expression ( ).",yes
PMC6077799,Figure_1,oa_package/5b/ea/PMC6077799.tar.gz,"[':H E 100, original magnification.']","Figure 1: H&E 100, original magnification. Tumor is shown to the right, circumscribed and surrounded by a thin fibrous capsule (arrows).",yes
PMC4869465,Figure_1,oa_package/43/5d/PMC4869465.tar.gz,"['Slit lamp examination revealed a very shallow anterior chamber (AC) with peripheral iridocorneal touch and an inferotemporal corneal perforation [].', 'Right eye anterior segment photograph showing an inferotemporal corneal perforation with a shallow anterior chamber and peripheral iridocorneal touchThe patient underwent urgent corneal application of cyanoacrylate glue and placement of a bandage contact lens with consequent deepening of the AC.']",Figure 1 Right eye anterior segment photograph showing an inferotemporal corneal perforation with a shallow anterior chamber and peripheral iridocorneal touch,yes
PMC2782140,Figure_1,oa_package/ab/74/PMC2782140.tar.gz,"['In UAS-APP-expressing lines, antibodies against the N-terminal region of human APP (22C11) detected full-length forms of APP695 [A-22C11(APP-N), elav; APP].', 'Double transgenic lines coexpressing human APP and BACE resulted in the partial loss of the larger APP band [A-22C11(APP-N), elav; APP/BACE].', 'Immunoprecipitation of SDS-protein extracts from the heads of elav; APP/BACE transgenics with monoclonal antibody 4G8 revealed A monomer and SDS-stable A oligomers [A-\n\n4G8(IP), elav APP/BACE].', 'Immunohistochemistry demonstrated that A deposits in the Drosophila brain were detected mainly in the outer cellular cortex layer that contains neuronal and glial cells in flies simultaneously expressing APP and BACE [B-b,d; elav; APP/BACE].', 'g001Human APP expression in the brain of transgenic Drosophila.']",10.1371/journal.pone.0008191.g001,yes
PMC2700555,Figure_6,oa_package/4a/f9/PMC2700555.tar.gz,"['As seen in OVA-treated RBP-jCKO animals exposed to three doses of intranasal OVA, K14-TSLPtg mice developed a severe asthmatic response and significant lethality, which were not seen in the control group ().', 'Histological examination of lungs from surviving K14-TSLPtg animals revealed pronounced airway remodeling with goblet cell hyperplasia and inflammation around the lung airways and vasculature (A), which were negative for TSLP expression prior to OVA treatment (Diagnosis in childhood is difficult and usually made on the basis of epidemiologic data.']",Fig. 5. Ranke complex. Mildly calcified right hilar lymph node (arrowhead) and tuberculoma (arrow) manifesting as a solitary round pulmonary lesion in a child with TB.,yes
PMC3339553,Figure_1,oa_package/39/c2/PMC3339553.tar.gz,"['In breast ductal carcinoma in situ (DCIS) with comedo lesions, layers of transformed epithelial cells fill up the ductal space and due to intra-lesional hypoxia a central necrotic core arises (A).', '.', 'Hypoxia impaired both polarization and lumen formation as demonstrated in MCF-10A cells (B and C) (Vaapil et al.']","Figure 1. A: Ductal carcinoma of the breast, the comedo form, with several cell layers of epithelial cells surrounding a central necrotic area. The inner cell layers, adjacent to the necrosis, show low differentiation with unorganized structures and increased nucleus-to-cytoplasm ratio (scale bar: 500 m). B: Non-malignant mammary epithelial cells cultured in a three-dimensional differentiation-inducing assay. After 21 days of culture at normoxia (21% O ), the mammary epithelial cells (MCF-10A) differentiate into growth-arrested, organized acini structures with polarized cells surrounding a hollow lumen, resembling the mammary gland appearance. The differentiated mammary cells have a polarized expression pattern of proteins, here laminin V (green), and small compact nuclei (blue, actin in red) in a palisade structure. C: At hypoxia (1% O ) the MCF-10A mammary epithelial cells fail to arrange into organized structures and appear as cell aggregates without lumen or polarized protein localization. The hypoxic cells have larger nuclei, remain proliferative, and express markers of undifferentiated cell stagecharacteristics often seen in breast carcinoma (scale bar: 20 m).",yes
PMC3817605,Figure_1,oa_package/08/1e/PMC3817605.tar.gz,"['MiRNAs as Tumor SuppressorsMiRNAs can act as tumor suppressors when their reduced expression or loss of function contributes to the development of a malignant cell phenotype [3] ().', 'MicroRNAs as tumor suppressors and oncogenes.', 'MiRNAs as OncogenesMiRNAs act as oncogenes when their increased expression or gain of function contributes to the development of a malignant cell phenotype [57] ().']","Figure 1 MicroRNAs as tumor suppressors and oncogenes. ( ) In normal cells, miRNA transcription, processing and binding to complementary sequences in the target mRNA lead to the repression of their target genes, by either mRNA translation inhibition or mRNA degradation. ( ) The reduced expression of a miRNA that acts as a tumor suppressor, as a result of chromosomal deletion or defects at any stage of miRNA biogenesis (indicated by question marks) leads to the increased synthesis of the miRNA-target oncoprotein (purple squares), and ultimately to the development of an oncogenic phenotype. ( ) The increased expression of a miRNA that acts as an oncogene, as a result of (among others) amplification of the miRNA gene or constitutive promoter activation (indicated by question marks), leads to the repression of a miRNA-target tumor-suppressor gene (pink), which favors the development of an oncogenic phenotype. ORF: open reading frame; mGpppG: 7-methylguanosine. Reproduced with permission from [ ].",yes
PMC6240072,Figure_3,oa_package/ce/c2/PMC6240072.tar.gz,"[' 3a).', ' 3b), and glutamine-derived (M + 5) -KG (P 0.', ' 3c).', ' 3d).', ' 3e).', ' 3f, g) and increased cell death in Pkd1 / cells (P = 0.', ' 3h) relative to the controls.', ' 3h).', 'Metabolic rearrangement in bioenergetics pathways and glutaminolysis rewiring in Pkd1-/- cells.', 't-test (b, c, and d) and (g and h, comparison between each condition)Following the fate of the 15N2-labelled glutamine we noticed that there was a significant increase (P = 0.']","Fig. 3 Metabolic rearrangement in bioenergetics pathways and glutaminolysis rewiring in cells. The scheme illustrates the fate of glutamine C atoms in Krebs cycle intermediates (Oxidative). Cells were incubated in glutamine-free DMEM supplemented with 4mM C N glutamine for 24h. Quantification of the intracellular labelled C N glutamine (M+7)showing that cells have a higher uptake compared to the controls. Isotopologue distribution of intracellular -KG shows that pools containing five C (M+5), coming from labelled glutamine, were all significantly higher in as compared with cells. Isotopologue distribution of intracellular succinate, fumarate, and malate shows that pools containing four C (M+4), coming from labelled glutamine, were all significantly higher in as compared with cells. Graphs are means in percentages relative to control cells of six technical replicates from one experiment. statistical significance is provided for total pool. Representative graph showing the results in percentage of the XFMitoFuel Flex Test analysis measuring OCR in response to either glucose or glutamine from at least seven technical replicates per well of two independent experiments. Data show a reduced oxidation of glucose in cells and an increased dependency on glutamine compared to wild-type cells. Cells were grown under starvation fromeither glucose or glutamine or bothin 10% serum for 24 and 48h after an overnight 0.5% serum. Representative images showing the cellular morphology of compared to cells in starved and non-starved conditions. Viability of and cells, expressed as percentage cell count compared to time 0, after 24h of incubation in starvation fromglucose, glutamine, or both. Percentage of apoptotic cells assayed by TUNELassay, showing significant higher percentage of apototic cells in starvation. Graphs ( ) are representative of at least three independent experiments, data are means from at least three technical replicates. MeanSEM were indicated, n.s. not significant ( 0.05), * <0.05; ** <0.01; *** <0.001; **** <0.0001. -test ( , , and ) and ( and , comparison between each condition)",yes
PMC10494846,Figure_1,oa_package/2c/0f/PMC10494846.tar.gz,"['.', 'The results showed that compared with the WT group, the blood glucose levels and glucose intolerance at each time point were higher, the insulin levels were significantly increased, and TG and TC were also increased in the T2D group and T2D+SPD group, Interestingly, compared with T2D group, there were no differences of the above indexes in the T2D+SPD group, also SPD had no effects in the WT group (A 1F).']","Figure 1. db/db mouse was selected as the research object of the type 2 diabetes model, and the related indexes were determined on 12 weeks: (A) Scheme of the in vivo experiment; (B) blood glucose concentration of different time point; (C) insulin resistance test; (D) serum insulin concentration; (E) triacylglycerol; (F) total cholesterol. * < 0.05 vs WT group ( 8). WT: Wild type; SPD: Spermidine; T2D: Type 2 diabetes.",yes
PMC10898641,Figure_1,oa_package/06/a6/PMC10898641.tar.gz,"['The most common pathology diagnosed was mature teratoma, followed by haemorrhagic cysts, endometriomas and luteal cysts [], as well as serous and mucinous cyst adenomas.', '5)SD: Standard deviation(a) Huge ovarian cyst that complicated pregnancy in early gestational age, (b) Huge ruptured endometrioma with acute abdomen, (c) A large multiloculated mucinous cystadenoma at 14th week of gestation(a) Abdominal pain due to large right ovarian cyst at 16th week of pregnancy, (b) Acute abdominal pain due to ovarian torsion at 16 weeks of gestation, (c) Acute abdominal symptoms due to ovarian torsion at 17th week of pregnancyDISCUSSIONOvarian cyst management during pregnancy can be a challenge for either an obstetrician or a patient.']","Figure 1 (a) Huge ovarian cyst that complicated pregnancy in early gestational age, (b) Huge ruptured endometrioma with acute abdomen, (c) A large multiloculated mucinous cystadenoma at 14 week of gestation",yes
PMC6189686,Figure_4,oa_package/ce/27/PMC6189686.tar.gz,"['All our patients received medical treatment base on dual antiplatelet therapy, statin, betablockers, and angiotensin-converting enzyme inhibitor with good outcome (s 4 7).', '003""/>A coronary angiography image showing spontaneous dissection of mid-Right coronary artery.']",Figure 4 A coronary angiography image showing spontaneous dissection of mid-Right coronary artery.,yes
PMC2213237,Figure_7,oa_package/92/b8/PMC2213237.tar.gz,"['07% of the CD4+ and CD4 population, respectively ().', '.']","Figure 7. Tracking of donor lymphocytes in recipient mice. 4 10 CD4 or CD4 MLNC from infected mice were transferred i.v. into C57BL/6 (Ly5.2) recipients, 7 d before airway challenge. After the second airway challenge, lungs and thoracic lymph node (TLN) cells were recovered and stained for Ly5.1 and CD4 expression. Error bars show the mean SEM.",yes
PMC6495951,Figure_6,oa_package/73/7b/PMC6495951.tar.gz,['Schematic diagram illustrates the mechanism of TGF 1 regulation of Cx43 in chondrocytes.'],"Figure 6 Schematic diagram illustrates the mechanism of TGF1 regulation of Cx43 in chondrocytes. The green lines point to Smaddependent pathways elucidated by the current study, and the blue dotted lines are potential mechanisms not included in the present study. TGF1 induced the canonical Smad signalling pathway, the activated TRI phosphorylates the RSmads (Smad2, Smad3), transform them into transcriptional coregulator together with Smad4, and dock to potential binding site of promoter of Gja1 which encodes Cx43. As a result, Cx43 accumulated more on cell border and phosphorylated into functional gap junctions with larger size. Cx43, connexin43; TGF, Transforming growth factor1",yes
PMC10409867,Figure_3,oa_package/a1/77/PMC10409867.tar.gz,"['We detected a shift toward a pathogenic phenotype in HSC and LSEC (): Over time, there was an increase in CD34 expression in peri-portal, sinusoidal, or peri-venular regions, and in -SMA expression diffusely.']","FIGURE 3 Shift toward a pathogenic phenotype in HSC and LSEC over time. By comparing similarly-sized portal tracts, central veins, and sinusoids in the index LB versus last follow up histology , there was an increase in CD34 expression in peri-portal, sinusoidal, or peri-venular regions, and in -SMA expression diffusely.",yes
PMC10560438,Figure_2,oa_package/87/a6/PMC10560438.tar.gz,"[' 2A, C E).', ' 2A, C E).', 'Epalrestat inhibits canonical and noncanonical NLRP3 inflammasome activation but has no effect on NLRC4 and AIM2 inflammasome activation.', '001; ns, not significantCaspase-11 can be activated by intracellular LPS or gram-negative bacteria to target noncanonical NLRP3 inflammasome activation.', ' 2A, C E).', ' 2A, C E).', ' 2B, F H).', ' 2B, F H).']","Fig. 2 Epalrestat inhibits canonical and noncanonical NLRP3 inflammasome activation but has no effect on NLRC4 and AIM2 inflammasome activation. LPS-primed BMDMs were treated with or without epalrestat (40M) before stimulation with ATP, nigericin, SiO , or poly(I: C) and Pam3CSK4-primed BMDMs were treated with or without epalrestat (40M) before stimulation with LPS. Immunoblot analysis of epalrestat was used to detect thecleaved caspase-1 and production of IL-1 in the Sup and the expression of caspase-1 p45, pro-IL-1, and ASC in the Lys. LPS-primed BMDMs were treated with or without epalrestat (40M) before stimulation with ATP, poly (dA: dT), or . Immunoblot analysis of epalrestat was used to detect cleaved caspase-1 and IL-1 in the Sup and the expression of caspase-1 p45, pro-IL-1, and ASC in the Lys. The activity of caspase-1 , , secretion of IL-1 , , and the production of TNF- , in the Sup were assessed from samples described in or . Data are presented as the meanSD from at least three biological samples. Statistical differences were analyzed using an unpaired Students -test. *P<0.05, **P<0.01, ***P<0.001; ns, not significant",yes
PMC7573948,Figure_2,oa_package/0c/69/PMC7573948.tar.gz,"['Higher power magnification showed bland looking spindle cells arranged in interlacing fascicles with no atypia or mitosis seen ().', 'Low power image showing compact spindle tumor involving the rete-testis, picture in right square is a higher power of tumor showing bland looking spindle cells arranged in interlacing fascicles with no atypia or mitosis seen.', '']","Fig. 2 Low power image showing compact spindle tumor involving the rete-testis, picture in right square is a higher power of tumor showing bland looking spindle cells arranged in interlacing fascicles with no atypia or mitosis seen.",yes
PMC11556108,Figure_2,oa_package/ca/d6/PMC11556108.tar.gz,"[' 2).', ' 2A).', ' 2A,C) and resulted in similar trends.', '', 'n = 3 6 animalsFull and partial recovery of LSECs fenestrations in 3-month-old and 6-month-old control and Mcpip1 KO mice in response to cytochalasin BFenestrations are dynamic structures that are continuously formed, moved within the cell to be finally fused within minutes to hours [45].', ' 2).', ' 2A).', ' 2B).']","Fig.2 Comparison of LSEC porosity measurements in Mcpip1 KO and control mice using AFM and SEM. Representative scanning electron microscopy images of LSECs isolated from 3-month-old and 6-month-old mice. The porosity of LSECs expressed as a fenestrae frequency, i.e. the number of fenestrations per area of cell, as measured using atomic force microscopy (AFM) and scanning electron microscopy (SEM) for GA-fixed cells. Additionally, the effect of 30 min treatment with 21 M cytochalasin B was presented, showing weaker responsiveness, i.e. induction of less fenestrae in the Mcpip1 KO group. Each point represents a single cell. The box charts show mean2575% of data points and the whiskers 5% and 95%. * <0.05; ** <0.01, *** <0.001. Additionally, # indicates significance of <0.01 after cytochalasin B treatment versus respective non-treated genotype. =36 animals",yes
PMC3749290,Figure_1,oa_package/5a/73/PMC3749290.tar.gz,"['1"">Detection of emphysema in patients with CF by MDCTDifferent patterns of emphysematous lesions were observed in CF patients with increasing age and severity of lung disease ().', 'With increasing EI, more voxels were found along bronchovascular structures with an emphasis on the lung periphery (A,B).', 'High EI resulted in extensive involvement of the parenchyma with a spread to the perihilar region (C,D).', 'Some CF patients with advanced lung disease showed a centrilobular and paraseptal emphysema pattern (E G).', 'g001Visualization of emphysema distribution in cystic fibrosis (CF) patients by chest MDCT density masks.', 'In the present study, we demonstrate that CF patients develop significant emphysema in addition to airway mucus plugging and bronchiectasis ().']",10.1371/journal.pone.0073142.g001,yes
PMC11196015,Figure_2,oa_package/ec/e9/PMC11196015.tar.gz,[' The 3D TEE shows the PVL (20 x 14 mm) defect around the bioprosthetic valve causing severe TR.'],"Figure 2 The 3D TEE shows the PVL (20 x 14 mm) defect around the bioprosthetic valve causing severe TR. TEE:Transesophageal echocardiography, PVL: Paravalvular leak, TR: Tricuspid regurgitation",yes
PMC7600189,Figure_5,oa_package/07/90/PMC7600189.tar.gz,"['The tissues from sham rats showed no histological alterations and the muscle fibers maintained its integrity ((A,A1), see histological score F).', 'The CAR group showed disorganization in the structure of the muscle fibers, with an increase in the space between the fibers ((B,B1)), see histological score F) and a significant increase in inflammatory cells infiltration, which confirmed the positive course of the inflammatory reaction caused by the induction of edema.', 'Rats with AE extract, at a dose of 10 mg/kg still showed a significant infiltration of inflammatory cells, edema formation, and muscle fiber disorganization ((C,C1), see histological score F).', 'However, treatment with AE extracts at the dose of 30 and 100 mg/kg significantly improve the normal structure of the fibers and showed an important reduction of inflammatory cells ((D,D1) and (E,E1) respectively; see histological score F) in a dose dependent manner.', 'Effect of AE at different doses on paw edema volume following CAR-injection in rats.']","Figure 5 Effect of AE at different doses on paw edema volume following CAR-injection in rats. No histological variations were detected in group 1: Sham + vehicle rats ( ) and ( ), see histological score ( ). Extensive histological damage was evaluated in group 2: CAR + vehicle rats ( ) and ( ), see histological score ( ). Group 3: CAR + AE 10 mg/kg has been ineffective in reducing CAR tissue damage ( ) and ( ), see histological score ( ). CAR tissue damage was reduced by AE + 30 mg/kg ( ) and ( ), see histological score ( ) and AE + 100 mg/kg ( ) and ( ), see histological score ( ) treatments. Data are representative of at least three independent experiments; One-Way ANOVA test. *** < 0.001 vs. sham; ## < 0.01 vs. CAR; ### < 0.001 vs. CAR. ND not detectable.",yes
PMC7317050,Figure_1,oa_package/2a/89/PMC7317050.tar.gz,"['At least 14 cases where both GIST and pheochromocytoma occurred in NF1 patients have been reported in the literature,11,51 all pheochromocytomas were adrenal in location ().', '52,53.']","Figure 1. Imaging characteristics of abdominal neoplasms in NF1 patients. Axial postcontrast T1-weighted fat-saturated MR image demonstrates an infiltrative enhancing plexiform neurofibroma in the proximal left obturator region with components extending into the perineum and pelvic floor (A). Axial fused PET/CT image of the plexiform neurofibroma demonstrates low levels of FDG uptake with SUV 3.63.8; however, no definite areas for were suspicious for malignant transformation (B). Axial T2-weighted MR image demonstrates a heterogeneous mixed solid and cystic mass in the left retroperitoneum arising from the left L3 nerve root. Core biopsy was performed and pathology was consistent with malignant peripheral nerve sheath tumor (MPNST) (C). Axial and coronal postcontrast T1-weighted fat-saturated images demonstrate heterogeneous enhancement of the periphery and soft tissue components of the MPNST (D and E). Axial postcontrast CT image demonstrates a heterogeneous right adrenal mass in keeping with pheochromocytoma. There is also infiltrating low-attenuation soft tissue around the imaged portal structures (F). Coronal postcontrast CT image further demonstrates the infiltrating periportal soft tissue mass, in keeping with a plexiform neurofibroma. There is also patulous distension of the duodenum with oral contrast. In the proximal jejunum, there is a soft tissue mass in keeping with gastrointestinal stromal tumor (GIST) which was subsequently resected (G). Axial postcontrast CT image demonstrates routine surveillance of further intraluminal masses in the distended proximal duodenum, in keeping with further GISTs (H).",yes
PMC2039791,Figure_2,oa_package/35/f2/PMC2039791.tar.gz,"[' 2)bReference for fresh brain weights [12]cReference for fixed brain weights [15]Tissue preparation and immunocytochemistryFormalin-fixed, paraffin-embedded tissue was sectioned at 6 m and mounted on organosilane-coated slides (SIGMA, St Louis, MO, USA).', ' 2 shows the pedigree, which links patients 4, 5 and 6 to a common ancestry.', '', ' 2Pedigree linking patients 4, 5 and 6 in the present report; IX, 1 and IX, 3 were reported before [5]Cerebellar cortexThe cerebellar hemispheres are severely affected in all.']","Fig.2 Pedigree linking patients 4, 5 and 6 in the present report; IX, 1 and IX, 3 were reported before [ ]",yes
PMC7478031,Figure_2,oa_package/85/76/PMC7478031.tar.gz,"['Blisters and haemorrhagic bullae were seen on the medial aspects of both legs and the soles of the feet (figure 2).', 'Image on admission showing blisters and haemorrhagic bullae on the medial aspects of the leg.']",Figure 2 Image on admission showing blisters and haemorrhagic bullae on the medial aspects of the leg.,yes
PMC9372195,Figure_2,oa_package/a6/f7/PMC9372195.tar.gz,"['This was supported by enlargement of right lung soft tissue mass, involvement of more mediastinal lymph nodes and thoracic vertebrae (A).', 'Local biopsy tissues showed small cells with crush artifact in hematoxylin-eosin staining, and were positive for TTF-1, Syn, CD56, Ki-67 with LI of 100%, P53 with LI of 40% in immunohistochemical staining (B-2D).', 'Radiological and histopathological images in early Dec 2020.']","Figure 2 Radiological and histopathological images in early Dec 2020. (A) Mass enlargement was shown in chest CT scan reevaluation. (B) Right distal bronchi tissues were positive for malignant cells with small size in hematoxylin-eosin staining (100). (C) Right distal bronchi tissues were positive for Syn in immunohistochemical staining (100). (D) Right distal bronchi tissues were positive for Ki-67 in immunohistochemical staining (100). CT, computed tomography; Syn, synaptophysin.",yes
PMC3610658,Figure_2,oa_package/a0/58/PMC3610658.tar.gz,"['2%; P105L, 0%; A).', 'The concentration of tau protein in CSF was highest (14,215 15,058 pg/mL) in patients with M232R-rapid and lowest (711 214 pg/mL) in patients with P105L (B).', '5% or greater in patients with P102L-CJD, V180I, E200K, or M232R-rapid but less than 15% in patients with GSS (shaded in B).', '8%; C); these rates are comparable to those of patients with sporadic CJD (sCJD) Participation of NADPH oxidase in P.']","Figure 6 Participation of NADPH oxidase in -induced ROS, iNOS/NO, and COX-2/PGE expressions. (a) Flow cytometric analysis of ROS fluorescence intensity in NPCs infected with for 24 hours, pretreated with or without DPI (10 M). The infection+DPI group compared with the infection group. (bd) DPI inhibited -induced iNOS/NO and COX-2/PGE production in NPCs. The infection+DPI group compared with the infection group; < 0.05. values were analyzed by one-way ANOVA. Data are presented as the meanSD from three independent experiments.",yes
PMC10441467,Figure_2,oa_package/9a/87/PMC10441467.tar.gz,"[' illustrates the brain atrophy trajectory for each subtype, with the w-score ranging from 1 to 3, indicating the degree of brain atrophy from mild to moderate to severe.', 'The Limbic-predominant subtype showed more atrophy in its key regions, the BASC-identified clusters 9 and 12 (, Additional file 1: ', 'Subtypes progression patterns identified by SuStaIn algorithm.']","Figure 2 Subtypes progression patterns identified by SuStaIn algorithm. (a) W-scores of subtype progression patterns for each region for each subtype. Color shade represents the probability that w-score in each region is reached at each SuStaIn stage, with red for mild atrophy (w-score = 1), magenta for moderate atrophy (w-score = 2), and blue for severe atrophy (w-score = 3). (b) Spatial distribution and degree of cortical atrophy at each SuStaIn stage. Color shades represent the cumulative sum of probabilities in each brain region.",yes
PMC7482978,Figure_2,oa_package/29/47/PMC7482978.tar.gz,"['Previously, CT () and MRI images had been taken and the radiologist found small calcifications in her right parotid gland but no sign of parotitis, cysts or fistula.', 'CT scan taken prior to the first visit.']","Figure 2 CT scan taken prior to the first visit. There can be seen subcutaneous induration, but no cysts nor fistula was obvious.",yes
PMC11141914,Figure_5,oa_package/6b/1a/PMC11141914.tar.gz,"['qPCR and immunoblotting confirmed KD and OE (, A, B, E, and F) with no effect on cell viability or superoxide production (Supplemental , A and B).', 'Scratch assay also showed delayed wound healing with PPIF KD, while OE accelerated wound healing (, C, D, G, and H).', '2), with a large majority of these genes being upregulated (I).', 'Gene set enrichment analysis (GSEA) demonstrated that a large panel of ECM-related genes were decreased with PPIF KD (J).', 'Consistent with the microarray gene expression data and GSEA, EnrichR analysis showed an increase in TGF 1 expression, signifying the importance of PPIF in the epithelial-mesenchymal interaction (, K and L).', 'Consistent with this, PPIF KD and NIM811 treatment inhibited TGF- induced phosphorylation of SMAD2 as shown by immunoblotting (, M P), while PPIF OE displayed the opposite (, Q and R).', 'Scratch assay using SB-431542, a small molecule inhibitor of TGF- , increased phosphorylation of SMAD2 and induced the inhibition of keratinocyte migration (S).', 'Wound healing subsequently increased when used in combination with the CyD inhibitor NIM811, further strengthening the possibility that CyD works through the TGF- signaling pathway in keratinocytes (Supplemental ).', 'Cyclophilin D promotes keratinocyte migration and affects expression of extracellular matrix genes.']","Figure 5 Cyclophilin D promotes keratinocyte migration and affects expression of extracellular matrix genes. ( and ) qPCR (mean SEM) and immunoblot verification of gapmer-mediated CyD KD compared with negative gapmer in keratinocytes. ( and ) Scratch cell migration assay in keratinocytes with gapmer-mediated KD in the presence of mitomycin C. = 3; 2-tailed, unpaired test. ( and ) qPCR (mean SEM) and immunoblot verification of vector-mediated OE compared with pCMV in keratinocytes. Two-tailed, unpaired test. ( ) Scratch cell migration assay in keratinocytes with OE in the presence of mitomycin C. One-way ANOVA. ( ) Heatmap illustration of microarray analysis for differentially expressed genes after KD (fold change < 1.2 or > 1.2, with < 0.05). Data were hierarchically clustered. Blue, lower; red, higher. = 3 biological replicates. ( ) GSEA heatmap and enrichment score showing a change in extracellular matrixmaintaining genes. ( and ) Molecular pathways identified by Enrichr software to understand the most important mechanism affecting the keratinocyte migration. ( ) Immunoblot and quantification (mean SEM) of phosphorylated SMAD2 in vehicle and NIM811-treated ( and ), negative and gapmer ( and ), and pCMV and PPIF OE keratinocytes ( and ). Two-tailed, unpaired test. ( ) Scratch cell migration assay in keratinocytes with NIM811 and SB-431542 treatment in the presence of mitomycin C. **** < 0.0001; ** < 0.01; * < 0.05.",yes
PMC7434530,Figure_6,oa_package/b0/6e/PMC7434530.tar.gz,"['JNK3 expression was detected via qRT-PCR and Western blot (A and B).', 'As we can see, depletion of JNK3 reduced JNK3 levels along with suppressed cell growth (C and D) and metastasis (E and F) in the two prostate cancer cell lines.', 'As shown in G, the percentage of apoptotic JNK3 knockdown cells was dramatically inhibited compared to that in the negative control.', 'JNK3 regulates the tumorigenesis of prostate cancer cells.']","Figure 6 JNK3 regulates the tumorigenesis of prostate cancer cells. JNK3 was knocked down by two separate siRNAs in two prostate cancer cell lines. After 48 h, the expression of JNK3 was detected by qRT-PCR ( ) and Western blot ( ). Cell growth was measured by CCK8 assay in PC-3 ( ) and DU145 cells ( ). Cell migration was detected by wound healing assay ( ). Cell invasion was detected by Transwell assay ( ). Cell apoptosis was measured by FACS ( ). *p<0.05, **p<0.01 compared to the corresponding controls.",yes
PMC5473895,Figure_1,oa_package/d7/15/PMC5473895.tar.gz,"[' 1).', ' 1 (left panel); 4,689 1,366).', ' 1).', ' 1).', ' 1).', 'Immunodepletion of CCL5 alleviated inflammatory hepatic IRI.', '\nInhibition of hepatic IRI by CCL5 receptor antagonistAn in vivo competition against CCL5 for receptor binding was further performed using Met-CCL5, an antagonist of CCR1 and CCR5 receptor.', 'Consistent with our earlier observations with exogenous manipulation of anti-CCL5 or Met-CCL5 availability (Figs 1 and 2), these results confirm that CCL5 directly contributes to the severity of hepatic IRI.']","Figure 1 Immunodepletion of CCL5 alleviated inflammatory hepatic IRI. C57BL/6C mice were subjected to ischemia for 60minutes and received anti-CCL5 antibody via jugular vein immediately after reperfusion. Mice were sacrificed at 24hours after reperfusion. Mice administered with either IgG control or anti-CCL5 Abs (1, 10, and 100ng) for immunodepletion of CCL5. Both the serum ALT and tissue MPO activities decreased significantly in a dose dependent manner (MeanSD, n=8). CTR, control. ns=not significant at >0.05; * <0.05; ** <0.01, *** <0.0001.",yes
PMC10748135,Figure_2,oa_package/49/ba/PMC10748135.tar.gz,"['An MRI of the spine revealed intramedullary dorsal column signal hyperintensities stretching from C2 to Th11, consistent with demyelination (see ).', 'T2-weighted contrast-enhanced spine MRI shows subacute combined degeneration.']",Figure 2 T2-weighted contrast-enhanced spine MRI shows subacute combined degeneration. Contrast enhancement was observed in the cervical ( ) and thoracic ( ) spinal cord. Investigations were performed two days after admission.,yes
PMC10627612,Figure_3,oa_package/b2/5e/PMC10627612.tar.gz,['.'],Figure 3. Diagnostic laparoscopy findings (AF). Left reproductive vessel. Left vas deferens. Left spermatic cord. Left ectopic testis in the right groin region. Mullerian duct tissue. Right hernial sac. Hydrocele of tunica vaginalis.,yes
PMC2899457,Figure_4,oa_package/37/d7/PMC2899457.tar.gz,[' Sustainable levels of FUS and TDP-43 mRNA in the ventral horn of spinal cord.'],"Figure 4 Regular PCR analysis showing that the mRNA levels of FUS and TDP-43 were sustainable in the ventral horn of spinal cord throughout mouse lifetime. Lumbar spinal cords were dissected from C57BL6 mice at varying ages and cut into transverse sections. The ventral horns were dissected from the tissue sections and pooled for RNA extraction. , Quantitative PCR analysis showing that the FUS and TDP-43 genes were sustainably expressed in the ventral horn of spinal cord in mouse lifetime. The detection thresholds for FUS and TDP-43 were first normalized to those for L17 mRNA and were then calculated relative to those in the mice at the age of 10 days. Data were the means averaged from three mice at each defined age.",yes
PMC10667884,Figure_2,oa_package/1d/20/PMC10667884.tar.gz,[],"Image 2 Coronal CT of the abdomen and pelvis showing distended hepatic flexure with wall thickening (yellow arrow), distended ascending colon with wall thickening (blue arrow), focal narrowing at the splenic flexure (red arrow) with distended terminal ileum (green arrow).",yes
PMC5561715,Figure_4,oa_package/ab/66/PMC5561715.tar.gz,"['(b) Post-operative picture, 20 weeks post-procedure showing almost complete repigmentation(a) Pre-operative picture.']",Figure 4 (a) Pre-operative picture. (b) Post-operative picture,yes
PMC8366622,Figure_2,oa_package/9e/e6/PMC8366622.tar.gz,"['To this end, we inactivated K562-S cells by either X-ray irradiation or treatment with formalin (FI) and administered them to C57/BL6 mice in comparison to untreated K562-S cells in a 4-week two-shot regimen consisting of S-encoding DNA vaccine (DNA-psv-S) as prime and K562-S as boost ((A)).', 'The three vaccination groups showed comparable anti-RBD IgG endpoint titers ((B)) and pseudovirus ID50 titers ((C)), suggesting minimal effect of both inactivation methods on the immunogenicity of K562-S.', 'The analyses revealed that IgG2c to IgG1 ratio was close to 1 for both the live K562-S group and K562-S-Xray group while slightly less than 2 for K562-S-FI group, consistent with a balanced T cell response ((D,E)).', '.']","Figure 2. The effect of inactivation on the immunogenicity of K562-S vaccines. (A) Experimental scheme. Female C57/BL/6 ( =6/group) mice were immunized at week 0 with either empty DNA vector (DNA-psv) or S-expressing DNA vector (DNA-psv-S), followed by booster immunization at week 4 with DNA-psv-S, parental K562 cells (K562-WT), or K562-S cells that were untreated (K562-S) or through inactivation by formalin (K562-S-FI) or X-ray irradiation (K562-S-X-ray). The dosages of DNA vectors and K562-S were 100g and 110 cells, respectively. (B and C) Serum antibody responses were assessed at week 6 post prime by a RBD-specific binding antibody ELISA (B) and pseudovirus neutralization assays (C). (D and E) Assessment of Th1 or Th2 bias in the immune response. The serum levels of IgG2c and IgG1 antibody were determined at week 6 post prime by ELISA (D) to calculate the IgG2c/IgG1 ratio (E), which is a reliable surrogate measure of Th1/Th2 status. Antibody titer data were presented as geometric mean titers (GMT)geometric standard deviation (GSD). KruskalWallis test with Dunns adjustment were applied when comparing groups. ns=no significant.",yes
PMC10786694,Figure_5,oa_package/c1/eb/PMC10786694.tar.gz,"['To assess the importance of Gal3 in tauopathies in vivo, we crossed Tau22 mice with Gal3 knockout mice (Tau22/Lgals3 / , A).', 'The knockout of Gal3 reduced the levels of misfolded tau (MC1-positive), aggregated tau (AT100-positive), and phosphorylated tau (AT8-positive) in the hippocampal CA1 region of Tau22, as assessed by immunofluorescence (, B E and Supplemental 9, A and B) and Western blot analyses (, F and G).', 'Compared with Tau22/Lgals3+/+ mice, Tau22/Lgals3 / mice exhibited reduced levels of the inactive/demethylated form of PP2A and decreased kinase activities of GSK-3 and CaMKII- (, F and G).', 'Importantly, the loss of Gal3 also prevented the learning and memory deficits present in Tau22/Lgals3+/+ mice (33) to a great extent, as assessed by the Morris water maze test (, H and I).', 'Our data showed that Gal3 knockout rescued the number of synapses assessed by the colocalization of VGLUT1 and Homer1 (, L and M and Supplemental 0, A and B).', 'Moreover, the deletion of Gal3 markedly reduced the extent of tauopathy in THY-Tau22 mice (, A E).', 'Collectively, the results indicated that the genetic deletion of Gal3 in Tau22 mice ameliorated major disease-related symptoms (), supporting the importance of GAM in tauopathy.', 'Loss of Gal3 rescues tauopathy in THY-Tau22 mice.']","Figure 5 Loss of Gal3 rescues tauopathy in THY-Tau22 mice. ( ) Schematic diagram illustrating the study design to identify the roles of Gal3 in Tau22 mice. IHC staining and quantification for ( and ) MC1 and ( and ) AT100 in mouse hippocampi, MC1: = 8 for Tau22/ , = 7 for Tau22/ ; AT100: = 5 mice. Each dot represents the average value of each animal. ( ) Immunoblot analysis of sarkosyl soluble mouse hippocampi (11 months) stained for MC1, AT100, Tau5, demethylated PP2A subunit C (inactive PP2Ac), PP2Ac, pGSK-3 Y216, GSK-3, pCaMKII-, and CaMKII-. ( ) Quantification of the data in , = 7 mice. ( ) Quantification of the time to platform in the 5-day training section of the Morris water maze, = 13 for WT, = 17 for , = 12 for Tau22/ , = 16 for Tau22/ . ( ), Quantification of the time spent in quadrant 4 (Q4, the quadrant with the hidden platform during the training section) versus all other quadrants (a.o., average of 3 other quadrants) on probe trial Day 8. Data are shown as the mean SEM. ( and ) IHC staining and quantification of CD68 in Tau22/ and Tau22/ mice, = 5 mice, 3 fields per animal. ( ) IHC staining of Homer 1 and VGLUT1 in the CA1 region of Tau22/ and control mice. ( ) Quantification of the staining in L, = 8 mice, 3 fields per animal. Scale bars: 500 m ( and ), 10 m ( ), 2 m ( ). Data in and were analyzed by 2-way ANOVA with Tukeys test. Other data were analyzed with a 2-tailed unpaired test, and all violin plots show the median with the 25th and 75th percentiles. * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001.",yes
PMC6642321,Figure_6,oa_package/41/bc/PMC6642321.tar.gz,['Transmission electron microscope images of proximal tubules and glomeruli in Cd exposed medaka.'],"Figure 6 Transmission electron microscope images of proximal tubules and glomeruli in Cdexposed medaka. Normal proximal tubule mitochondria (PTmit) (red arrowhead; A, C), normal PT apical membrane (PTapical) vesicle (yellow arrowhead; E, G), normal PT basolateral membrane (PTbasolateral; red asterisks; I, K), normal glomeruli podocytes (Glmpodocyte; red arrow; M, O), damaged PT mitochondria (light blue arrowhead; B, D), abnormal PT apical vesicles (green arrowhead; F, G), abnormal PT basolateral (light blue asterisk; J, L), enlarged podocyte foot process with apoptotic vesicles (light blue arrow; N, P). Female 0ppm Cd (A, E, I, M), female 4ppm Cd (B, F, J, N), male 0ppm Cd (C, G, K, O), male 4ppm Cd (D, H, L, P). The scale bar indicates 600nm (AD, MP) or 2m (EL).",yes
PMC5686467,Figure_4,oa_package/c7/4b/PMC5686467.tar.gz,"['The CT scan of abdomen demonstrates the Steel pan pattern of the sigmoid volvulus ().', 'CT Abdomen showing a Sigmoid Volvulus with Steel pan pattern.', '1.']",Fig. 4 CT Abdomen showing a Sigmoid Volvulus with Steel pan pattern.,yes
PMC11334229,Figure_2,oa_package/26/66/PMC11334229.tar.gz,[],,yes
PMC7780514,Figure_2,oa_package/e3/80/PMC7780514.tar.gz,"['In addition, TEE showed negative bubble study with and without Valsalva manoeuvre (figure 2).', 'The microbubbles produced by an agitated saline with a provocative test could not detect patent foramen ovale.']","Figure 2 The microbubbles produced by an agitated saline with a provocative test could not detect patent foramen ovale. LA, left atrium; LASP, left atrial septal pouch; RA, right atrium.",yes
PMC7850589,Figure_1,oa_package/89/65/PMC7850589.tar.gz,[],"FIGURE 1 A 62-year-old woman had progressively developing throbbing right neck pain for 1 year. The pain, radiation to the right suboccipital area, sometimes included breathlessness. The episodes became more frequent within the last 3 months. Symptoms improved after taking medication from a local clinic. To detect cancer, patient received FDG PET/CT, which showed an intraspinal cord FDG-avid calcified mass (arrow) at the level of the first cervical spine, SUVmax 7.9 (initial), 8.2 (delayed).",yes
PMC10243805,Figure_1,oa_package/09/a5/PMC10243805.tar.gz,"['ResultsWe identified 4 individuals with diffuse lymphatic disorders due to mosaic KRAS variants, including 3 with epidermal nevus syndromes (Table 1 and Supplemental ; supplemental material available online with this article; [25] a significant increase in the numbers of splenic CD4 Foxp3 nTreg was observed during the first 5 days of PyL infection (B) and this was accompanied by increased levels (MFI) of Foxp3 expression (C) and a transient increase (on day 3pi) in the nTreg/non-Treg ratio (D).', 'g002Expansion and activation of natural Foxp3 regulatory T cell populations during PyL and PyNL infections.', 'A representative plot showing Foxp3-GFP expression in infected and uninfected mice is shown in E.', 'Numbers of CD4 GFP cells were significantly increased in the spleen on 5 day pi (F) and on day 7 pi (data not shown) but did not differ significantly between PyL-infected and PyNL-infected mice.']",10.1371/journal.ppat.1000004.g002,yes
PMC6251271,Figure_4,oa_package/4d/c0/PMC6251271.tar.gz,"['The sensitivity was 100 per cent in diagnosing atrophy, polyp () and hyperplasia (', 'Hysteroscopic view of endometrial polyp.']",Fig. 4 Hysteroscopic view of endometrial polyp.,yes
PMC5500070,Figure_1,oa_package/2b/2d/PMC5500070.tar.gz,['Development of skin rashes after kidney transplantation.'],"Figure 1 Development of skin rashes after kidney transplantation. A and B, Three weeks after occurrence, skin rashes (arrow) were distributed on the face and abdomen. C and D, Six weeks after occurrence, skin rashes (arrow) were resolving.",yes
PMC6188173,Figure_2,oa_package/5f/18/PMC6188173.tar.gz,[' \n Axial MRI of lesion.'],Figure 2 Axial MRI of lesion. T1 weighted MRIof cervical spine with axial images showing a hypointense mass in posterior elements from C2 to C4 measuring approximately 3.5 cm x 1.7 cm x 1.6 cm.,yes
PMC5612612,Figure_4,oa_package/58/96/PMC5612612.tar.gz,"['003""/>DSC-MRI (a) and DCE-MRI (b) for differentiation of GBM, PCNSL, and metastasis.']","Figure 4 DSC-MRI (a) and DCE-MRI (b) for differentiation of GBM, PCNSL, and metastasis. rCBV maps demonstrate different characteristic features in the three distinct entities, with significantly higher rCBV value of GBM compared with metastasis and PCNSL. The value of GBM is significantly lower than metastasis and PCNSL. Reproduce with permission from Mangla et al. [ ], Xing et al. [ ], Zhao et al. [ ], and Kickingereder et al. [ ].",yes
PMC8648445,Figure_4,oa_package/48/62/PMC8648445.tar.gz,"['The image processed by the low-rank matrix denoising algorithm was clearer than before, as shown in .', '003"" position=""float""/>MRI images before and after low-rank matrix denoising processing (the red arrow indicated the lesion).']",Figure 4 MRI images before and after low-rank matrix denoising processing (the red arrow indicated the lesion).,yes
PMC4572858,Figure_1,oa_package/16/c9/PMC4572858.tar.gz,"['a shows that at 3 days after complete optic nerve\naxotomy, the intensity of the YFP label or the number of YFP-positive RGCs\ndid not differ from those in non-injured (intact) retinas (injured:\n63 4 RGCs; intact: 66 4 RGCs, mean S.', 'This time course of injury-induced RGC death\nwas confirmed using an antibody against the transcription factor Brn3a, an\nRGC-specific marker19 (b).', 'YFP /NF-H RGCs had medium to large monostratified dendritic\narbors with smooth dendrites lacking spines, a common feature of adult\nRGCs21 (s 1c e).', 'Following axotomy, RGC dendritic arbors\nwere visibly smaller than those in non-injured, intact neurons (s 1f and g).', 'Analysis of total dendritic\nlength and total dendritic area demonstrated a reduction of 15 \n(3707 m) and 25 (112 \n103 m2), respectively,\ncompared with control RGCs (length: 4320 m; area: 150\n 103 m2) (s 1h and i and Tables\n1 and 2).', 'Sholl analysis, which\nmeasures the number of dendrites that cross-concentric circles at increasing\ndistances from the soma, revealed a leftward shift indicating a reduced\narbor complexity in axotomized neurons (\n1j and Table 2).', '21307827RGC dendritic arbors retract soon after axonal injury and prior to cell\ndeath.']","Figure 1 RGC dendritic arbors retract soon after axonal injury and prior to celldeath. ( ) Quantitative analysis of RGC densities showed nosignificant change in the number of YFP-positive RGCs at 3 days afteraxotomy, whereas substantial neuronal loss was observed at 5 days afterlesion (axotomy 3 days: =7; axotomy 5 days: =4). ( ) A similar pattern of RGC loss was observedusing the RGC-specific marker Brn3a ( =3). The density ofRGCs in intact, uninjured retinas is shown as reference( =11). Data are expressed as meanS.E.M. (ANOVA,*** <0.001, =311 miceper group). ( ) YFP-positive RGCs that colabeled withan antibody against NF-H and had clearly identifiable axons (arrow) wereselected for dendritic arbor imaging and reconstruction. Scale bar:25 m. ( and ) Three days afteraxotomy, RGCs had visibly smaller dendritic arbors than non-injured, intactneurons (axotomy: =21 cells; intact: =17cells). Quantitative analysis of dendritic parameters revealed a significantreduction in total dendritic length ( ), dendritic field area( ) and arbor complexity ( , Sholl analysis). Values areexpressed as meanS.E.M. (Student's -test,** <0.005, * <0.05, cells wereanalyzed from 5 mice per group)",yes
PMC8392684,Figure_3,oa_package/c5/74/PMC8392684.tar.gz,"['Group Differences in Grey Matter Intensity displays profiles of grey matter intensity decrease in each patient group relative to Controls (see Table 3 for full details).', 'Regions of significant grey matter intensity decrease in right-predominant semantic dementia (SD-R: top panel; blue); left-predominant semantic dementia (SD-L: middle panel; yellow) and Alzheimer s disease (AD: bottom panel; green) relative to Controls.']","Figure 3 Regions of significant grey matter intensity decrease in right-predominant semantic dementia (SD-R: top panel; blue); left-predominant semantic dementia (SD-L: middle panel; yellow) and Alzheimers disease (AD: bottom panel; green) relative to Controls. Coloured voxels indicate regions that emerged as significant in the voxel-based morphometry analyses extracted voxelwise and corrected for family-wise error at < 0.05. ACE-III Total, years in education, and scanning site included as nuisance variables in the analyses. Clusters are overlaid on the Montreal Neurological Institute (MNI) standard brain with x and y coordinates reported in MNI standard space. R = right. Figures created using MRIcroGL.",yes
PMC10995756,Figure_3,oa_package/e0/6e/PMC10995756.tar.gz,[' Neck X-ray shows no presence of soft tissue air or any fluid collection.'],Figure 3 Neck X-ray shows no presence of soft tissue air or any fluidcollection.,yes
PMC10605327,Figure_1,oa_package/d5/a5/PMC10605327.tar.gz,"['Negative or weak nuclear staining were categorized as low expression, while moderate or strong nuclear staining were grouped as high expression ().', '1007/s12032-022-01876-936352293Representative pictures of YAP1 immunohistochemistry.']","Figure 1 Representative pictures of YAP1 immunohistochemistry. Nuclear YAP1 expression is evaluated and scored as negative (no expression) ( ), weak ( ), moderate ( ), and strong ( ) nuclear expression. Negative/weak expressions are considered as low-YAP1 expression, and moderate/strong expressions are considered high YAP1 expression. Scale bar: 100 m.",yes
PMC8611971,Figure_6,oa_package/fb/5b/PMC8611971.tar.gz,"[' 6a, b).', ' 6c).', ' 6d).', 'Lysosomal disruption with Chloroquine leads to increased pathology but not a change in distribution.', '01)DiscussionLBs are highly complex cellular structures, and our understanding of their composition is still developing [30].', ' 6c, d).']","Fig. 6 Lysosomal disruption with Chloroquine leads to increased pathology but not a change in distribution. Neurons treated in 96-well plates with 25ng per well of either AD1-P1 or PFF for 6h followed by a 30min pulse of either PBS or 25 uM Chloroquine (ChQ) and fixed after 10days. Scale bar 50m. Quantitation of total 81A signal for neurons treated as in . Same quantitation of total 81A as in , with totals for Somatic and Neuritic pathology shown. Percent of total 81A pathology in somatic inclusions from neurons treated as in . Totals are the average of 3 replicate wells. Error bars represent the standard deviation. Significance compared to PFF treated neurons was determined by student t-test (* <0.05, ** <0.01)",yes
PMC7383629,Figure_1,oa_package/30/7b/PMC7383629.tar.gz,['Reduction in proliferating astrocytes in dTg (APP23/GFAP TK) mice treated with ganciclovir (GCV).'],"Figure 1 Reduction in proliferating astrocytes in dTg (APP23/GFAPTK) mice treated with ganciclovir (GCV). (a) Schematic of the treatment. dTg mice were treated either with GCV or vehicle for 2 weeks at 9 months of age. (b) Representative images and quantification of GFAP staining in dTg mice in cortex and hippocampus; images were acquired using a 10 objective. (c) Representative images of a reduction in plaqueassociated astrocytes using triple staining for ThioS (green), GFAP (magenta), and Iba1 (red), and quantification of area of plaque associated astrocytes (GFAP) ( = 34) and microglia (Iba1) ( = 37) in cortex; images were acquired using a 10 objective. (d) Double staining of GFAP (red) and Ki67 (green) in the hippocampus of dTg mice treated with vehicle or GCV and quantification of the double labeled GFAP and Ki67 positive cells in cortex and hippocampus ( = 3); images were acquired using a 63 objective and the scale bar is 10 m. Values shown in graphs represent the mean value . Statistical analysis included oneway ANOVA with Tukey's multiplecomparison posttest. * <.05; ** <.01. ANOVA, analysis of variance; GFAP, glial fibrillary acidic protein; ThioS, ThioflavinS [Color figure can be viewed at ]",yes
PMC4640700,Figure_1,oa_package/37/ef/PMC4640700.tar.gz,"['ResultsInduction of islet death by pro-inflammatory cytokinesAcute overnight treatment of isolated mouse islets with a cocktail of pro-inflammatory cytokines (PIC; IL-1 /TNF- /IFN- ) induced cell death ().', 'Representative images are shown in A.', 'The data is quantified in C.', 'g001Single cytokine treatment is not sufficient to induce apoptosis in isolated mouse islets.', 'Representative images are shown in B.', 'The data is quantified in D.', 'Islets were incubated with each cytokine at the equivalent dose used in the PIC cocktail (one-fold; 1X) and at four-fold this dose (4X) (B and 1D).', '001) and no significant difference to control (D).', '01; relative to control; A and 1C).', 'Results from quantitative analyses of fluorescence (C) showed that cell death for triple cytokine treated islets (17.', 'These data correlate with the cell death studies ().']",10.1371/journal.pone.0142735.g001,yes
PMC10339868,Figure_5,oa_package/27/af/PMC10339868.tar.gz,"['Additionally, staining was noted in some interstitial cells (A D) (Supplementary Materials S1).', '004) for the controls (E).', 'We also identified a significant increase in the expression of LOX in the cardiomyocytes of HCM cats (E) which concurs with a recent finding in human HCM patients [27].', 'Myocardial expression of LOX (in brown) is increased in cats with HCM.']","Figure 5 Myocardial expression of LOX (in brown) is increased in cats with HCM. ( , ) LV tissue immunostained for LOX showed minimum labelling in the controls (N = 5), while increased labelling was observed in the HCM cats (N = 6). Bar = 200 m. ( , ) Images of higher magnification showed that the LOX immunostaining in the cardiomyocytes was markedly stronger in the HCM cats compared to controls. Some cardiac interstitial cells (cell type undetermined) also expressed LOX (open arrow heads). LOX (brown). Nuclei (blue). Bar = 50 m. ( ) Averaged area immunostained for LOX per field of view (%) was significantly higher in the HCM cats (dotted bar; N = 6) than the controls (N = 5). Data were analysed using Welchs -test and expressed as individual data points with median and IQR.",yes
PMC6031826,Figure_1,oa_package/36/d9/PMC6031826.tar.gz,"['(: Intestinal type adenocarcinoma : 3+ Positive).', '(a and b): Lauren s Intestinal subtype of adenocarcinoma (H E, x100 and x400), (c): HER2 IHC showing strong complete/basolateral membranous staining in 10% tumour cells - 3+ Positive (x400).', '7% of the diffuse type ( and ).']","Figure 1 (a and b): Laurens Intestinal subtype of adenocarcinoma (H&E, x100 and x400), (c): HER2 IHC showing strong complete/basolateral membranous staining in >10% tumour cells - 3+ Positive (x400).",yes
PMC9648657,Figure_5,oa_package/e4/3e/PMC9648657.tar.gz,"['If the hydrocephalus persists, a VPS is done [];iii.', 'F: Post-operative MDCT showing the craniotomy with distractor in situIllustrative case 3.']","Figure 5 Illustrative case 3. A child with Crouzons. He presented as an infant with hydrocephalus. At 3 months, he had an endoscopic third ventriculostomy. Subsequently, he had PCVD. Upper row: Post PCVD. He continued to have severe airway issues with repeated episodes of upper respiratory infections. He underwent frontofacial distraction using internal distractors. Middle row: Post frontofacial distraction. Lower row: Follow-up at 6 years. At follow-up, his aesthetic outcome seemed suboptimal, which is one of the disadvantages of early midface correction",yes
PMC4228456,Figure_4,oa_package/b7/e5/PMC4228456.tar.gz,['The morphology and immunohistochemistry confirmed the diagnosis of hemangioma of cavernous and venous mixed type in mesentery.'],"Figure 4 . The hemangioma was located in the mesentery and the small bowel wall was reserved. . The tumour was characterized by diluted vessels, which most was with thin wall and minor was with thick wall. . CD31 was positive in the endothelial cells flooring the vessels. . SMA was positive in some vessels with thick wall.",yes
PMC4097934,Figure_1,oa_package/8b/7f/PMC4097934.tar.gz,"['The hearts appeared healthy and not enlarged [a] and the valves were normal [b and c].', ' Control hearts appeared healthy and not enlarged (a) and the valves were normal (b, at white arrow) and (c)A large amount of fat surrounded the atria of the anti-nuclear antibodies (ANA) positive hearts (a) in comparison to the control hearts [] and the negative control hearts (b).']","Figure 1 Control hearts appeared healthy and not enlarged (a) and the valves were normal (b, at white arrow) and (c)",yes
PMC3742816,Figure_1,oa_package/22/c5/PMC3742816.tar.gz,"['5% (19/2; ).', 'YagiYFushidaSHaradaSBiodistribution of humanized anti-VEGF monoclonal antibody/bevacizumab on peritoneal metastatic models with subcutaneous xenograft of gastric cancer in miceCancer Chemother Pharmacol66745753201020033809.']","Figure 1. (A) Results of immunohistochemical staining for VEGF in ovarian cancer tissues. Microscopy showed that the tumor cell cytoplasm was stained, brown granules were visible and that consequently, VEGF expression was markedly positive. (B) Results of immunohistochemical staining for Flt-1 in ovarian cancer tissues. The tumor cells and stroma vessels were stained and the degree of vascular positive staining (arrows) was stronger. (C) Results of immunohistochemical staining for KDR in ovarian cancer tissues. Microscopy showed that brown granules were visible in the tumor cell cytoplasm, the nucleus was blue and that all the tumor cells were stained. VEGF, vascular endothelial growth factor. (AC) Magnification, 200.",yes
PMC10414799,Figure_6,oa_package/d1/2d/PMC10414799.tar.gz,['Point-of-Care UltrasoundThis point-of-care ultrasound demonstrates an aortic dissection flap in the longitudinal view.'],"Figure 6 Point-of-Care Ultrasound This point-of-care ultrasound demonstrates an aortic dissection flap in the longitudinal view. Image by Robert Jones, DO, FACEP, retrieved from The POCUS Atlas: on 9/15/2022. Content licensed under the Attribution-NonCommercial 4.0 International (CC BY-NC 4.0).",yes
PMC8246213,Figure_4,oa_package/4a/0c/PMC8246213.tar.gz,"['Under the fear of lung torsion/infarction or a new massive PE, an urgent CT Chest was conducted on the same day, which showed patent vessels to the lobe and a totally atelectatic lung with mucous plugging of the main airways ().', 'A CT chest of the patient was obtained on postoperative day 3 as the white out was refractory to any treatment up to that day.']",Figure 4 A CT chest of the patient was obtained on postoperative day 3 as the white out was refractory to any treatment up to that day. The scan showed totally atelectatic lung with some areas of aeration and patent main vessels.,yes
PMC2797586,Figure_6,oa_package/5b/18/PMC2797586.tar.gz,[],"Figure 6 Curve of local recurrence-free survival in months for the 24 patients with primary osteosarcoma that was non-metastatic at diagnosis, according to histological type",yes
PMC3664425,Figure_4,oa_package/99/59/PMC3664425.tar.gz,[],"Figure 4 Benign hyperplastic nodule in the transition zone in a 69-year-old patient with high serum PSA level of 9.2 ng/mL. Apparent diffusion coefficient (ADC) map. T2-weighted image. Scheme of systematic and targeted biopsies. ADC map (a) demonstrates a large oval low ADC (1.09 10 mm /sec) lesion (arrow) in the basal transition zone. The lesion (arrow) shows low signal intensity on T2-weighted image (b). The scheme of prostate biopsy (c) demonstrates the number, location, and course of all the biopsy specimens including eight systematic biopsy cores (gray arrows) and four targeted biopsy cores (black arrows). Histological examination proves the lesion to be hyperplasia in the targeted biopsy specimens.",yes
PMC9327355,Figure_6,oa_package/9d/42/PMC9327355.tar.gz,"['After advancement of the balloon catheter at the desired position, the balloon is inflated, and fibrinogen and thrombin solutions are separately injected through each catheter lumen under fluoroscopy until filling in the major portal branches ().', '.']","Fig. 6. Preoperative portal vein embolization for hilar cholangiocarcinoma A. A 4-F pigtail catheter was advanced into the portal vein via the left portal branch and portography was performed. B. A balloon catheter was advanced into the posterior branch of the right portal vein and a fibrin glue mixed with iodized oil was injected under balloon occlusion. C. Thereafter, the fibrin glue was also injected into the anterior branch of the right portal vein under balloon occlusion. D. Portography performed immediately after portal vein embolization (PVE) showed complete occlusion of the right portal vein. E. Unenhanced CT performed 1 week after PVE showed the fibrin glues mixed with iodized oil in the right portal branches.",yes
PMC10614797,Figure_6,oa_package/7c/c2/PMC10614797.tar.gz,"['To explore a potential protective role of let-7 miRNA in experimentally-induced emphysema, we generated mice which allowed for selective induction of let-7 activity in T cells using the published rtTA-iLet7 mice crossed to CD4-Cre (herein referred to as let-7GOF; A) (Angelou et al.', 'Providing further evidence of let-7-dependent regulation of Rorc, protein levels of ROR t were suppressed in CD8+ and CD4+ T cells of let-7GOF mice relative to controls (B).', 'The let-7GOF mice did not exhibit any signs of lung inflammation or pathologic remodeling at baseline (C,D and data not shown) Histopathologic analysis revealed a comparable degree of lung alveolar distension via morphometric measurements of MLI in nCB-treated let-7GOF mice versus controls suggesting that enforced let-7 expression is insufficient to protect the lung from emphysema (C,D).', 'On the other hand, evaluation of the IL-17+ response and ROR t levels in emphysematous lung T cells demonstrated that, in contrast to control nCB-treated mice, let-7GOF mice exhibited dampened lung Tc17 and Th17 cell populations and were resistant to the induction of ROR t after nCB-exposure (E,F).', 'Taken together, our let-7 LOF and GOF models demonstrate the necessity and sufficiency of let-7 miRNA to act as a molecular brake to the type 17 T cell response through the direct regulation of ROR t, further our data suggests that nCB- or CS-mediated suppression of this braking mechanism furthers inflammation and exacerbates emphysema severity (G).', '562059v3-f0005"" position=""float""/>.']","Figure 6. Enforced expression in T cells restrains induction of RORt and Tc17/Th17 inflammation in lungs of nCB-exposed mice. (A) Schematic outlining our T cell-inducible mouse model ( ). (B) Flow analysis of RORt expression in live, TCR CD8 or CD4 T cells from (B) nave control and mice in thymus, spleen, and lungs (n=3-5 per group). (C) Control and mice were treated with PBS vehicle or nCB then analyzed. Representative H&E-stained lung sections from PBS- and nCB-exposed mice as indicated on each panel (x20 magnification; scale bars, 50m) (D) MLI measurements from indicated mice (n=5-6 per group). (E) Flow analysis of lungs gated on live TCR CD8 or CD4 cells for (E) IL-17a population frequency (n=3-4 per group) or (F) RORt expression by representative flow plot and MFI quantification (n=4-5 per group). (G) Figure model for /RORt axis in emphysema pathogenesis. Data are representative of two (B) or three (C-F) independent experiments and displayed as meanSEM using students t-test (B) or two-way ANOVA with Tukeys multiple correction (D-F). *p < 0.05, **p < 0.01, ***p <0.001, ****p<0.0001.",yes
PMC4397049,Figure_2,oa_package/16/dc/PMC4397049.tar.gz,"['MRI revealed a tumor originating in the T9 vertebra and extending to the T8 and T10 vertebrae ().', '001""/>Preoperative MRI showing a multilevel invasive vertebral tumor with extraosseous extension.']","Figure 2 Preoperative MRI showing a multilevel invasive vertebral tumor with extraosseous extension. (a) Sagittal T2-WI, (b) axial gadolinium-DTPA-enhanced T1-WI image (T9).",yes
PMC5980993,Figure_4,oa_package/3f/67/PMC5980993.tar.gz,['Triggering receptors expressed on myeloid cells 2 (TREM-2) inhibited pyroptosis of infiltrating cells in Pseudomonas aeruginosa keratitis.'],"Figure 4 Triggering receptors expressed on myeloid cells 2 (TREM-2) inhibited pyroptosis of infiltrating cells in keratitis. Pyroptosis in the infected cornea assessed with terminal deoxynucleotidyl transferase-mediated uridine 5-triphosphate-biotin nick end labeling (TUNEL) staining. TUNEL-positive staining (green) was detected in versus wild type (WT) corneas at 5days after infection. Cell nuclei were stained with 4,6-diamino-2-phenyl indole (DAPI; blue). Magnification was 200 and 400 respectively. The protein levels of GSDMD and its N-terminal domain in versus WT B6 corneas were detected with western blot. The percentage of macrophages, polymorphonuclear neutrophils, and dendritic cells were detected by flow cytometry in the infected WT and corneas at 5days post infection. Data were shown to represent one of three individual experiments each with five mice per group.",yes
PMC6719923,Figure_4,oa_package/c8/5d/PMC6719923.tar.gz,"['Qualitatively, sections of both samples show a layered structure (a,b).', 'Relevant image acquisition data are reported in the figure data bar ().', '(a) SEM of pericardium control (P_CTRL).']",Figure 4 ( ) SEM of pericardium control (P_CTRL). ( ) SEM of pericardium treated with polyphenols (P_PRPE).,yes
PMC8544838,Figure_2,oa_package/54/6a/PMC8544838.tar.gz,"['RBCs infected with the parental parasite line CS2 showed normal knob morphology, with an even distribution of small knobs over the entire surface of the infected cell (A and S1 Video3D reconstruction and surface render of eKnob depicted in E and 2F.', '-Were specific length or width thresholds use to quantify knob morphology categories in C, 5D and S6B,D,E or was this done by eye?', '-Line 192: the lumen of these eKnobs was often extremely dense It appears that it is not the lumen (or core) but the tip of the eKnob that is electron dense by TEM while the lumen of the eKnobs shown in D, 3E and S3 is generally less dense.']",10.1371/journal.ppat.1009969.g002,yes
PMC9395532,Figure_2,oa_package/d9/86/PMC9395532.tar.gz,"[' 2a d and Supplementary ', 'Development of a transgenic mouse model (NeuLyso-Tag mouse) to isolate neuronal lysosomes from brains.', 'Pathogenic Syn species accumulate within neuronal lysosomes in mouse brains and primary neurons.']","Fig. 2 Development of a transgenic mouse model (NeuLyso-Tag mouse) to isolate neuronal lysosomes from brains. A lentiviral vector expressing LAMP1 via the neuron-specific synapsin-1 promoter was used to generate Tg- LAMP1 (or NeuLyso-Tag) mice via pronuclear injection of linearized DNA comprising the synapsin-1 promoter and the LAMP1 cDNA. Tg- LAMP1 mice were PCR-genotyped using primer-pairs at the N- and the C-terminal ends of the LAMP1 cDNA. Single insertion-site for the transgene was suggested by equal numbers of WT and Tg- LAMP1 pups born per litter ( =11 litters). There was also no effect of the transgene on birth-ratio and growth (indicated by weight gain; =5 per group) of the Tg- LAMP1 mice compared to WT mice. Immunoblots show that LAMP1 protein is detectable in mouse brains by immunoblots against the N-terminal HA tag and the C-terminal myc tag (representative of =7 littermates of each genotype). Histological colocalization of anti-HA immunofluorescence with NeuN staining in the cortex and hippocampus shows that LAMP1 construct is expressed in the neurons of Tg- LAMP1 mouse brains at 1 and 6 months of age (representative of >10 stained sections from =3 littermates of each genotype). All data represent meansSEM. n.s. not significant, by paired 2-tailed Students test for littermate ratio and by RM 2-way ANOVA for weight gain over age (each mouse matched over age). The FSW- LAMP1 lentiviral vector is described and tested in Supplementary Fig. , and further characterization of the Tg- LAMP1 mice is included in Supplementary Fig. .",yes
PMC10553844,Figure_1,oa_package/3e/02/PMC10553844.tar.gz,[' CT-guided lymph node biopsyA transverse section of the upper chest shows needle positioning for biopsy in the left axillary node in May 2021.'],Figure 1 CT-guided lymph node biopsy A transverse section of the upper chest shows needle positioning for biopsy in the left axillary node in May 2021.,yes
PMC3770590,Figure_3,oa_package/3a/ba/PMC3770590.tar.gz,"['g002""/>Bone Marrow HistologyMouse BM histology was examined in femurs from control and exposed mice after FA exposure (\n\n).', '0 mg/m3 dose, shown in F.', 'g003Effects of FA on bone marrow histology.']",10.1371/journal.pone.0074974.g003,yes
PMC8309054,Figure_5,oa_package/84/f7/PMC8309054.tar.gz,"['This observation was sustained by microscopic investigations that showed the presence of adhered cells at 24 h and 72 h post-PDT, most probably viable cells, even in the case of the stronger PDT regimen of 25 J/cm2 (A,B).', 'While the number of adhered cells decreased over time, a few cells that appeared to be less affected by PDT continued to slowly proliferate and formed cell islets at 120 h post-PDT (C).', 'Possibly, tumor cells develop rescue mechanisms that delay cell death and may even protect some cells against the deleterious action of PDT (C).', 'Images of HT29 tumor cells at various time points after PDT performed with various light fluences (10 25 J/cm2), which were delivered with the fluence rate of 50 mW/cm2.']","Figure 5 Images of HT29 tumor cells at various time points after PDT performed with various light fluences (1025 J/cm ), which were delivered with the fluence rate of 50 mW/cm . Cells were visualized by bright-field microscopy at 24 h ( ), 72 h ( ) and 120 h ( ) post-PDT (100 m scale bar).",yes
PMC8665564,Figure_4,oa_package/c8/a5/PMC8665564.tar.gz,"[' 4A, B).', ' 4C, D).', 'The sTREM2 fragment 41 81 increases the number of plaque-associated microglia.', 'D Quantitation of the number of plaque-associated microglia in the presence of sTREM2 fragment 51 81 (n = 5 mice, 47 plaques of Fc and 47 plaques of 51 81, paired Student s t test)The sTREM2 fragment 41 81 is more potent than the full-length sTREM2 in reducing the amyloid-related pathologyIt was later reported that the generation of sTREM2 is facilitated by the regulated shedding of membrane-bound TREM2 at the H157-S158 peptide bond [7 9].']","Fig. 4 The sTREM2 fragment 4181 increases the number of plaque-associated microglia. , Coronal sections from the 5xFAD mice injected with the Fc-tagged sTREM2 fragments ( , the sTREM2 fragment 4181; , the sTREM2 fragment 5181) or Fc alone were stained with DAPI (blue) for nuclei, MOAB-2 (green) for A, and Iba1 (red) for microglia. Representative z-stack images of the hippocampus regions are shown. Original magnification60; scale bar, 50m. Quantitation of the number of plaque-associated microglia in the presence of sTREM2 fragment 4181 ( =5 mice, 55 plaques of Fc and 43 plaques of 4181, paired Students test). Quantitation of the number of plaque-associated microglia in the presence of sTREM2 fragment 5181 ( =5 mice, 47 plaques of Fc and 47 plaques of 5181, paired Students test)",yes
PMC11383598,Figure_5,oa_package/24/25/PMC11383598.tar.gz,"['Another patient with PsA manifested prominent dactylitis in 4 digits, and mice injected with her PBMCs and sera developed several diffusely swollen digits (, A and B, right) with prominent infiltration by immune cells in the subcutaneous tissue (C) and considerable accumulation of proliferating type 1 T cells (, D and E).', 'Dactylitis in hu-PsA mice.']","Figure 5 Dactylitis in hu-PsA mice. Front limbs from hu-HC, hu-PsO, and hu-PsA mice were collected on day 30 after sera and PBMC injection. ( ) Diffuse third and fourth digit swelling or dactylitis was noted in hu-PsA mouse with higher areas of soft tissue inflammation. ( ) Tissue sections stained with Alcian blue show extensive inflammation in a hu-PsA mouse that correlated with dactylitis phenotype in a patient with PsA. Alcian Blue images were taken using an Olympus VS120 scanner. ( ) Inflammatory areas in soft tissue areas were measured with a QuPath 3.0 tool. Scale bar: 1,000 m. Data are shown as the average SD, = 4 mice. Two-way ANOVA and Tukeys multiple comparison test was used to calculate significance. ** 0.005. ( and ) Immune infiltrating cells were more numerous in hu-PsA mice than in hu-HC and hu-PsO mice and were mainly proliferating CD3 T cells (red) expressing the Ki67 proliferative nuclear marker (white) and T-bet (green). The graph shows the number of proliferative CD3 T cells in tissue sections from hu-HC ( = 4) and hu-PsO ( = 4) mice and a hu-PsA (3 random dactylitis areas) mouse. Representative IF area from of 20 20 mosaic images were taken (original magnification, 200) with a Zeiss Axioplan microscope and recorded with a hamamatsu camera. Scale bar: 200 m. Significance was calculated with 2-way ANOVA and Tukeys multiple comparison test. **** 0.0005.",yes
PMC3844243,Figure_2,oa_package/64/ba/PMC3844243.tar.gz,"['001""/>Fifty-eight-year-old female patient with kidney transplant in the right pelvic region.']","Figure 2 Fifty-eight-year-old female patient with kidney transplant in the right pelvic region. MRI scan showed multifocal, wedge-shaped signal alterations clearly in DWI-b800 (arrow) while T2w imaging could only depict slight pathologic signal (triangle). The diagnosis of nephritis was finally confirmed by urine analysis and laboratory findings.",yes
PMC5141618,Figure_7,oa_package/86/75/PMC5141618.tar.gz,"['This radiological term can be applied to teeth with developing roots and also in the cases of completely mineralized teeth ( ()).', '[15] ( (), Table 5 (Tab.']",Table 7 Alveolar bone loss of lower wisdom teeth (No. 38 and 48),yes
PMC7640573,Figure_1,oa_package/20/db/PMC7640573.tar.gz,"['Electrocardiogram (EKG) showed sinus rhythm with necrosis sequelae in inferior and basal territory, inverted T waves in V4 toV6, and some monomorphic ventricular extrasystole (VES) ().', 'Interv2011779039Electrocardiogram showing extensive posterior repolarization disorders (inferior, basal and lateral territory).']","Fig. 1 Electrocardiogram showing extensive posterior repolarization disorders (inferior, basal and lateral territory).",yes
PMC3496962,Figure_1,oa_package/f6/ce/PMC3496962.tar.gz,"['A second immediate CT scan at the time of wheezing showed simultaneous acute narrowing of the trachea and both main bronchi (A F).', 'A subsequent CT scan taken 10 minutes after administration of inhaled albuterol and resolution of wheeze showed resolution of size changes back to baseline (G I).', 'References1CzajaPSojaJGrzankaPCmielASzczeklikASladekKAssessment of airway caliber in quantitative videobronchoscopyRespiration2007744432438171645412OharaTHiraiTSatoSLongitudinal study of airway dimensions in chronic obstructive pulmonary disease using computed tomographyRespirology2008133372378183998593SchuellerGNeumannKHelbichTBronchial reactivity in hyperresponsive patients and healthy individuals: demonstration with high resolution computed tomographyEur J Radiol2004522151156154890724Beigelman-AubryCCapderouAGrenierPAMild intermittent asthma: CT assessment of bronchial cross-sectional area and lung attenuation at controlled lung volumeRadiology2002223118118711930065Acute bronchospasm on a computed tomography scan.']","Figure 1 Acute bronchospasm on a computed tomography scan. ( ) Baseline computed tomography images of the trachea, carina, and main bronchi. ( ) Computed tomography images during acute bronchospasm at the trachea (arrow up), carina (arrow sideways), and main bronchi (right bronchus: arrow down). ( ) Computed tomography images of the trachea, carina, and main bronchi 10 minutes after the administration of albuterol, which show resolution back to baseline.",yes
PMC4337123,Figure_9,oa_package/c1/de/PMC4337123.tar.gz,"['About two-thirds have intermediate to high signal on\nT2-weighted MRI, while the remaining demonstrate low signal intensity ()(23).', 'Pheochromocytoma (arrows).']","Figure 9 Pheochromocytoma (arrows). A large, heterogeneous adrenal mass on a T2-weightedMR image ( ). Cystic and solid enhancing components are depictedin a post-contrast MR image ( ). Elevated serum catecholamineswere detected in this patient with pheochromocytoma.",yes
PMC8590403,Figure_2,oa_package/d0/64/PMC8590403.tar.gz,[],"Figure 2 Mammography, ultrasound, and ultrasound-guided core needle biopsy from a left breast mass (BI-RADS 3), which was proved by pathology to be a fibroadenoma",yes
PMC11502566,Figure_6,oa_package/f9/2d/PMC11502566.tar.gz,"['Ipsilateraler H matopneumothorax und HerzbeuteltamponadeDes Weiteren ist auch auf Gasansammlungen zu achten, da diese abh ngig von Luftmenge und Lokalisation als Todesursache betrachtet werden k nnen.']","Abb. 6 Zugehrige postmortale Computertomographie (PMCT) im Weichteilfenster, axial. Weichteildefekt an der rechten Brustkorbvorderseite ( ). Ipsilateraler Hmatopneumothorax und Herzbeuteltamponade",yes
PMC5476281,Figure_1,oa_package/85/15/PMC5476281.tar.gz,"['To exclude possible artifacts in the tissue, VIP immunoreactive axons were defined by a minimum of three closely aligned immunoreactive spots (see for representative pictures).', 'Islets were identified based on autofluorescence and morphology (see C and C and Supplementary .']","Fig. 1 Early degradation of KA-induced gamma oscillations in mice. Representative diagram of LFP electrode location in CA3 region of hippocampal acute slices. Representative sample traces of LFP recordings from WT (left side) and (right side) at 1m.o., 2m.o., 4m.o. and 6m.o. Summary of normalized gamma oscillation power at 1m.o. (WT =5 vs =4, =0.9724), 2m.o. (WT =9 vs =10, =0.0006), 4m.o. (WT =6 vs =8, =0.0006) and 6m.o. (WT =6 vs =8, =0.0006) for WT (gray bars) and (red bars) showing a strong degradation in mice from 2m.o. onwards. Summary of normalized frequency variance at 1m.o. ( =0.7254), 2m.o. ( =0.036), 4m.o. ( =0.0102) and 6m.o. ( =0.0051) for WT (gray bars) and (red bars) showing loss of regularity of gamma oscillations in from 2m.o. onwards. Representative power spectra from WT (gray lines) and (red lines), at the different ages analyzed. Data in bar graphs are presented as meanSEM. indicates the number of mice. Statistics from two-way ANOVA followed by a HolmSidaks multiple comparisons test (Supplementary Table ). * <0.05, ** <0.01, *** <0.001.",yes
PMC10645150,Figure_2,oa_package/33/71/PMC10645150.tar.gz,"['The POSTN protein structure comprises an N-terminal EMI domain, a carboxy-terminal domain (CTD), and a tandem repeat of four fasciclin 1 (FAS1) domains (54 56) ().', 'Structure of POSTN as an ECM protein as a conventional ECM protein, POSTN maintains tissue and organ structure or generates fibrosis, whereas as a matricellular protein, it is involved in cell activation.']","Figure 2 Structure of POSTN as an ECM protein as a conventional ECM protein, POSTN maintains tissue and organ structure or generates fibrosis, whereas as a matricellular protein, it is involved in cell activation. POSTN comprises an EMI domain at the N terminus, four tandemly aligned FAS1 domains in the middle, and splicing domains at the C terminus. ECM proteins or a proteinase that can bind to the EMI domain or the tandem repeat of four FAS1 domains are depicted. In contrast, POSTN binds to integrin molecules on the cell surface, transducing intracellular signals. POSTN, periostin; ECM, extracellular matrix; FAS1, fasciclin 1.",yes
PMC10450518,Figure_2,oa_package/95/3d/PMC10450518.tar.gz,"['', '3Stat-3Weak immunostaining was observed in FL and PL fibroblasts and fibrochondrocytes (b, d, and f).', 'On the other hand, CL (b, d, and f) and HCL (', '2b, c, d, e, and g) expressed Stat-3 in the nucleus while the ossification area (c, e, and h) was positive in cytoplasm of osteoblasts and the extracellular matrix.', 'Cells of the articular disc presented Stat-3 essentially in the nucleus of fibroblasts, fibrochondrocytes, and cells surrounding blood vessels (i and j).', 'Mandibular fossa showed Stat-3 immunoexpression mainly in undifferentiated cells cytoplasm of proliferative layer (k and l).', 'Stat-3 was observed in osteoblasts cytoplasm surrounding the recently formed bone matrix (k and l) (See Table 1).', 'S1']","Fig. 1 Expression of Nanog protein (al). a General TMJ image with mandibular condyle (MC), articular disc (AD) and temporal bone (TB). b and d Mandibular condyle with fibrous (FL), proliferative (PL) and chondrocytes layers (CL). d and f- Chondrocytes layer (CL) of mandibular condyle. e and g- Hypertrophic chondrocytes layer (HCL) of mandibular condyle with nuclear staining (arrow). c, e and h Bone formation (BF) in the mandibular condyle (MC), with ostoblastic expression (arrow). i and j Articular disc showing imunostaining surrounding vessels (arrow). k and l- Mandibular fossa of temporal bone (TB) with expression in osteoblasts (*), fibroblasts of dense connective layer (DCL) and in undifferentiated cells of proliferative layer (PL) (arrow). a-Bar=500mb, c, i and k - Bar=200md, e, j and l-Bar=100m. f, g and h- Bar=50m.",yes
PMC8274119,Figure_4,oa_package/a3/ea/PMC8274119.tar.gz,"['Different lesions typically arise in each of the 3 compartments of the mediastinum, and this allows one to use the location of a lesion to shape the differential diagnosis of a mediastinal mass ().', '\n2\n\n.', '1177_23742895211021980-fig4"" position=""float""/>What Additional Tests or Procedures May Assist in the Evaluation of an Anterior Mediastinal Mass?']","Figure 4. Specific lesions tend to arise in each of the mediastinal compartments, mostly corresponding to the structures normally found in each area. LAD indicates lymphadenopathy.",yes
PMC8602945,Figure_2,oa_package/df/d9/PMC8602945.tar.gz,"['.', 'Statistical analyses were performed by a one-sided Wilcoxon rank-sum test in C, ']","Fig. 2. (A) PCA (with the top-2000 most-variable genes) of the trophoblast cell models: TOs ( =4), TO-EVT ( =4), TSC-2D ( =5), TSC-3D ( =5), TSC-EVT ( =5) and TSC-SCT ( =5). A representative image from each sample type is shown. (B) A heatmap of specific markers for each trophoblast subtype from the human first-trimester placenta. The markers are divided into the following groups: pan-trophoblast, SCT, cell column niche and EVT. Hierarchical clustering is shown based on log2 normalized expression. (C) Expression of classical HLA class I genes in TSC-2D, TSC-3D and TOs. Expression levels of and are significantly higher in TSC-2D compared with TSC-3D ( =0.028; =0.048) and TOs ( =0.018; =0.008). No significant differences were seen for transcripts of . Data are means.e.m. (D) PCA of small RNA (miRNA) profiles between TSC-2D ( =3), TSC-3D ( =5) and TOs ( =4). (E) Volcano plot of differentially expressed miRNAs between TSC-2D and TSC-3D. miRNAs that are targets of are highlighted (five in total). (F) Expression levels of the five specific miRNAs that target in TSC-2D, TSC-3D and TOs. Significance in C and F estimated by one-sided Wilcoxon test (* <0.05; >0.05). Scale bars: 500m in A.",yes
PMC10669520,Figure_9,oa_package/4d/b9/PMC10669520.tar.gz,"['To determine if other known components of the rod signaling pathway undergo a change in expression that correlates with the development of a rod response to A d/t, we analyzed their expression levels using 1-dimensional immunoblotting normalized to GAPDH as the internal loading control ().', 'Expression of proteins in the PrPC/NOX-dependent rod signaling pathway during i3Neuron development.']","Figure 9 Expression of proteins in the PrP /NOX-dependent rod signaling pathway during i Neuron development. ( ) Example of immunoblots for specific proteins and GAPDH loading control. ( ) Quantified protein expression data come from immunoblots of extracts from triplicate cultures ( = 3). An aliquot of one sample from each harvesting day was used to make the example summary gels in (A). Band intensities were normalized to GAPDH and are expressed relative to amounts at Day 0. The CXCR4 blots are performed with an antibody against the 47 and 49 kDa subunits, and the CCR5 blots are performed with an antibody against the 40 kDa subunit. * < 0.05.",yes
PMC5641992,Figure_1,oa_package/04/e5/PMC5641992.tar.gz,"[' 1A, B).', '1A, B).', '1C, the mass spectrum from leptomeningeal blood vessels in the subarachnoid space, arterioles, and cerebral parenchyma displayed different mass numbers corresponding to each A ion.', ' 1C).', '1A, B).', '1D).', 'MALDI-IMS for frozen AD brain sections.', 'This figure is a magnification of the region within the dotted square in A.', 'C: MALDI Mass spectrum in leptomeningeal blood vessels (LMV), arterioles (Ao), and senile plaque (SP) of B.', 'A 1 36 to A 1 41 are preferentially deposited in leptomeningeal blood vessels, while A 1 42 and A 1 43 are deposited in the cerebral parenchyma as senile plaques\nDeposition of full-length A s visualized in human brain with MALDI-IMSThe CAA phenotypes of case No.', '1A, E and Additional file 1: S3).', '1E).', '1A and E, MALDI-IMS with 100 m pitch resolution was used, and we obtained an overall distribution profile in a relatively wide area.', '1C).', '1C).', '1C).', '1A, B), it is plausible that the structures of its C-terminus structure predefine the dynamics of A s rather than those of the N-terminal end.', '1C and Additional file 1: s S5 and S6).', '1C and E, we demonstrated the presence of A 1 41 peptide in leptomeningeal vessels similar to the full length A species, A 1 36 to A 1 40.']","Fig. 1 MALDI-IMS for frozen AD brain sections. : A140 deposits in the leptomeningeal blood vessels and arterioles (red) and A142 deposits in cerebral parenchyma (green). The 4939.9 was used to detect the tissue structure and shows an unknown biomolecule (blue). : Optical density for MALDI-IMS. This figure is a magnification of the region within the dotted square in Fig. 1A. A140 is deposited in leptomeningeal blood vessels (1 and 5) and arterioles (4) shown in red. A142 is deposited in cerebral parenchyma as senile plaques (2 and 3) shown in green. : MALDI Mass spectrum in leptomeningeal blood vessels (LMV), arterioles (Ao), and senile plaque (SP) of Fig. 1B. A140 and N-terminal truncated Ax-40 are located in Ao, while A136 to A141 are in LMV. A142, A143, and N-terminal truncated Ax-42 are preferentially located in SP. : MALDI-IMS and IHC of various C-terminal truncated A peptides in AD with severe CAA. ( ) MALDI-IMS 100m resolution imaging for A140 (red) and A 142 (green). ( ) Highlight 20m resolution leptomeningeal blood vessels and cortex imaging in dotted square ( ). ( ) Highlight of an arteriole in solid square ( ). Adjacent sections of the occipital cortex from AD brains were immunostained and focused on arteriole and cerebral parenchyma ( ) using antibodies against A40 ( : BA27) or A42 ( : anti-A42 polyclonal) and merged view ( ). Both analyses demonstrated that A40 is preferentially deposited in leptomeningeal blood vessels and arterioles in the subarachnoid space and the cerebral parenchyma forming CAA. In contrast, A42 is mainly deposited in SP. IHC analysis also demonstrated the differential distribution of A40 and A42, which were CAA dominant and SP dominant deposition, respectively. Solid rectangles indicate the area illustrated in the panel. Scale bars=100m. : MALDI-IMS of various C-terminal truncated A peptides in AD with severe CAA (NO. 3). A136 to A141 are preferentially deposited in leptomeningeal blood vessels, while A142 and A143 are deposited in the cerebral parenchyma as senile plaques",yes
PMC11377139,Figure_2,oa_package/e3/81/PMC11377139.tar.gz,"['16 If a renal tumor met any of the following three criteria on cross-section CT, the contour of that layer was determined as irregular: 1) A mass with smooth but distorted margins, described as lobular with an arc-shaped focal convex protrusion arising from part of the tumor (', ' 2A B); 2) A mass with unsmooth and sharp nodules, usually small with an acute margin (', ' 2C D); 3) A mass with blurred margins, where the margin between the tumor and renal parenchyma is unclear (', '']","Fig.2 The contour irregularity with three criteria on cross-sectional imaging. (A and B) A mass with smooth but distorted margins, described as lobular with an arc-shaped focal convex protrusion arising from a part of the tumor (arrow). (C and D) A mass with unsmooth and sharp nodules (arrow), usually small with an acute margin. (E and F) A mass with blurred margins, where the margin between the tumor and renal parenchyma is unclear.",yes
PMC5520279,Figure_10,oa_package/08/3a/PMC5520279.tar.gz,[],Figure10 A patient who presented with symptoms of mass effect from a partially thrombosed vertebrobasilar transitional aneurysm was initially treated with five telescoped Pipeline embolization devices placed in the left vertebral artery. The patient was due to have coil occlusion of the contralateral vertebral artery but refused further treatment and later died from progressive mass effect.,yes
PMC7750402,Figure_8,oa_package/81/9a/PMC7750402.tar.gz,['The postoperative radiographs show defect reconstruction with bone substitute.'],Fig. 8 The postoperative radiographs show defect reconstruction with bone substitute.,yes
PMC9955517,Figure_10,oa_package/5f/10/PMC9955517.tar.gz,[],"Figure 10 Dupuytrens disease. ( ) Axial T1-weighted MR image demonstrating a low-signal mass measuring 9.6 mm 5.4 mm consistent with the diagnosis of superficial palmar fibromatosis or Dupuytrens disease. ( ) Axial fat-suppressed PD-weighted MR image demonstrating the low-signal nodule (yellow arrow); high collagen content within the nodule results in the relative hypointensity of superficial fibromatosis. ( ) Axial color-coded T2 map MRI demonstrates the nodule (black arrow) as reflecting tissue with decreased T2 relaxation times (blue color), the characteristic of collagen-rich fibromatosis.",yes
PMC7696051,Figure_4,oa_package/89/5e/PMC7696051.tar.gz,"['AE1/AE3 cytokeratin staining on control pancreas tissue (A,B) and ductal adenocarcinoma cases (C,D); A,C 20 , B,D 40 .']","Figure 4 AE1/AE3 cytokeratin staining on control pancreas tissue ( , ) and ductal adenocarcinoma cases ( , ); , 20, , 40.",yes
PMC11483584,Figure_2,oa_package/dc/47/PMC11483584.tar.gz,"['10"">Workflow of tau gradient consensus analysisTau gradient analysis was introduced for investigating whether there is a consistent trend across multiple samples in the tau deposition gradient, as observed in the representative data of C.', 'Whole-brain analysis of tau plaque pathology in rTKhomo mice.', ' (A) A scheme of searching tau gradient consensus across grouped regions in the data from the 47 regions within the 3D ROI determined in C from three 18-month-old rTKhomo mice.', 'Tau12 and Tau46) showed constant levels of tau protein in the TBS-extractable fraction (Supplementary A C).', 'AT8 and PHF1) showed an intense band that migrated to 68 kDa (indicated by arrow in Supplementary A) in the same fraction from 15-month-old (Supplementary ', 'The 68 kDa tau appeared at 12- to 15-month-old and increased with age (Supplementary F J).', 'AT8 immunostaining revealed the hyperphosphorylated intraneuronal tau inclusions distributed in the forebrain region (A).', 'In B, integrated tau signal intensity per regional volume across 53 medium-sized subregions were aligned from higher to lower levels (left to right).', '40 Higher levels of AT8-positive tau signals were observed in ECT and the agranular insular area (AI) of the isocortex, PIR of the olfactory area and the retrohippocampal region (RHP) of the Hippocampal formation (B).', 'C showed the density of the 157 subregions within the medium-sized regions above the mean signals.', 'As shown in the high-magnified images (C), AT8-postive tau signals were remarkably increased in ENTI, ENTm and BMA, indicating further confirmation of the unique distribution pattern of tau pathology in rTKhomo mouse brains.', 'To uncover the brain-wide pathological tau distribution, recent advances in whole brain three-dimensional (3D) staining and imaging50 had revealed that AT8-positive tau pathology in rTKhomo mice predominantly along the entorhinal cortex-hippocampal projection extended from ENT to various brain regions, including TEa, ECT, PRE, subiculum (SUB), DG and anmon s horn (CA1, CA2 and CA3) ().', 'In fact, the exact site of tau deposition in the whole brain () would not have been detectable by conventional immunohistochemistry without the use of 3D imaging.']","Figure 1 ( ) Western blotting of human tau and -actin protein in buffer-extractable fractions from 2-month-old rTKhetero mice (Tau+/, tTA+/), Tau-KI responder mice (Tau+/, tTA/) and wild type mice (Tau/, tTA/). Each genotype = 3 female. See for uncropped blots. ( ) Human tau immunoreactivity labelled by Tau12 antibody in sagittal brain sections from 2-month-old rTKhetero mice, Tau-KI responder mice and wild-type mice. Scale bars = 500m. ( ) DAPI stained, IBA1 immunofluorescence and GFAP immunofluorescence images of dentate gyrus from wild type and tTA tg on the congenic B6 background, and from wild type and tTA tg on 129 FVB F1 hybrid background. Scale bars = 100m. ( ) Areas of dentate granule cell layers in wild type and tTA tg on the congenic B6 background ( = 3 females each), and from wild type and tTA tg on 129 FVB F1 hybrid background ( = 3 females each). Averaged areas were taken from three serial sagittal sections of each mouse. ( ) Ratios of IBA1-positive signals in the dentate gyri. 3 females per group. ( ) Ratios of GFAP-positive signals in the dentate gyri. 3 females per group. **** < 0.0001 (Tukeys multiple comparison). ( ) Upper panels show AT8 immunofluorescence staining of hippocampus region from 18-month-old rTKhomo (Tau+/+, tTA+/) mouse ( ) and 6-month-old rTg4510 mouse ( ). Lower panels show conformation-specific tau antibody MC1 immunofluorescence staining of hippocampus region from 18-month-old rTKhomo mouse ( ) and 6-month-old rTg4510 mouse ( ). Inboxes show tau antibody-positive neurons in pyramidal cell layers of CA1. Scale bars = 200m.",yes
PMC7718647,Figure_1,oa_package/2e/58/PMC7718647.tar.gz,"['1Clinical pathology and lncRNA UBE2R2-AS1 expressionCell invasion and migration were aggravated in cervical cancer tissues positively correlated with the increasing stage (a).', '01, b).', 'Clinical pathology and lncRNA UBE2R2-AS1 expression.']","Figure 1 Clinical pathology and lncRNA UBE2R2-AS1 expression. Adjacent: adjacent normal tissues; III stage: III stage cervical cancer tissues; IIIIV stage: IIIIV stage cervical cancer tissues. (a) Clinical pathology by HE staining (200) and lncRNA UBE2R2-AS1 mRNA expression. ** < 0.01 and *** < 0.001, compared with NC group. (b) lncRNA UBE2R2-AS1 expression by ISH assay (200) ** < 0.01, *** < 0.001, compared with NC group.",yes
PMC10559940,Figure_1,oa_package/2f/ae/PMC10559940.tar.gz,"["" Section of Meckel's diverticulum and adjacent bowel on scanning power (4x).""]",Figure 1 Section of Meckel's diverticulum and adjacent bowel on scanning power (4x). 1. Area of ileal tissue 2. Area of smooth muscle 3. Several endometrial glands and stromal tissue within the wall of the diverticulum.,yes
PMC5826463,Figure_5,oa_package/8a/13/PMC5826463.tar.gz,"['Additional radiological workup included barium esophagram, which demonstrated an anterior impression on the esophagus ().']","Fig.5 Lateral view from fluoroscopic images obtained during a barium esophagram at 9 weeks old demonstrates a fixed anterior impression on the esophagus, marked with an arrowhead.",yes
PMC9356575,Figure_2,oa_package/d5/8b/PMC9356575.tar.gz,"['A representative example of CT qualitative score and CT quantitative assessment is shown in 2.', ' 2CT qualitative score and CT quantitative analysis.']","Figure2 CT qualitative score and CT quantitative analysis. 29-year-old male patient with COVID-19. (A) CT demonstrates multifocal ground-glass opacities and regions of consolidation in the right lower. The qualitative score established by a radiologist is based on the percentage of lung involvement per lobe (shown on the right, range 020). (B) CT quantitative analysis using segmentation software. Quantitative analysis extracts volumetric measurements (shown on the right) representing the aerated lung, the ground glass opacities (GGO) volume, the consolidation volume and the GGO to aerated lung ratio. RUL: right upper lobe; RLL: right lower lobe; ML: middle lobe; LUL: left upper lobe; LLL: left lower lobe.",yes
PMC4741470,Figure_1,oa_package/50/47/PMC4741470.tar.gz,"['Relative expression levels of IL-4, IFN and lymphotoxins and in infected NTx and d3Tx mouse stomachs in comparison with expression levels in NI miceExpression levels for infected NTx (n = 40) and d3Tx (n = 29) mouse groups were normalised in comparison with respective NI NTx (n = 10) and NI d3Tx (n = 8) control group expression levels.']","Figure 1 Relative expression levels of IL-4, IFN and lymphotoxins and in infected NTx and d3Tx mouse stomachs in comparison with expression levels in NI mice Expression levels for infected NTx ( = 40) and d3Tx ( = 29) mouse groups were normalised in comparison with respective NI NTx ( = 10) and NI d3Tx ( = 8) control group expression levels. Data are plotted as bar graphs displaying the average/mean standard deviation for each group, * < 0.05, ns = not significant. was used as housekeeping gene. Similar results were obtained with (not shown).",yes
PMC9240296,Figure_4,oa_package/b3/b6/PMC9240296.tar.gz,"['BD = bile duct, MDT = multidisciplinary team, RFA = radiofrequency ablationA 73-year-old female with rectosigmoid junction cancer.']","Fig. 4 A 73-year-old female with rectosigmoid junction cancer. A 5-cm ulceroinfiltrative mass (arrows) is seen at the rectosigmoid junction on a colonoscopic image. Colonoscopic biopsy confirmed well-differentiated adenocarcinoma (not shown). Axial and coronal contrast-enhanced CT images show well-enhanced wall thickening in the rectosigmoid junction colon (white arrows) and adjacent pericolic LN enlargement (arrowheads). The left distal ureter is encased by the enlarged LN, leading to left hydronephrosis (red arrows). On a F-FDG PET/CT image, colon wall thickening (arrow) and an adjacent enlarged LN (arrowhead) show strong FDG uptake suggesting rectosigmoid junction cancer with a pericolic metastatic LN. On an FDG PET/CT image at the thoracic level, a few FDG-avid LNs (arrows) are observed at the left axillary area. The nuclear medicine doctor interpreted these LNs as metastatic LNs. Therefore, palliative chemotherapy was planned. However, after a thorough image review by a dedicated gastrointestinal radiologist during a MDT discussion, the radiologist circumspectly noticed that there was an FDG-avid lesion at the left deltoid area (arrowhead) and therefore suggested the possibility of reactive FDG uptake after COVID-19 vaccination. After an MDT conference, clinicians confirmed that the patient received a COVID-19 vaccination 18 days before FDG PET imaging. Therefore, the management plan for the patient was changed to radical surgery. COVID = coronavirus disease, FDG = flurodeoxyglucose, LN = lymph node, MDT = multidisciplinary team",yes
PMC4660494,Figure_3,oa_package/ba/bd/PMC4660494.tar.gz,[],Figure 3 Postoperative X-ray of a first patient showing posterior instrumentation with kyphosis correction with trans pedicular grafting,yes
PMC10347741,Figure_3,oa_package/ba/3c/PMC10347741.tar.gz,"[' 3), with the patient referring remarkable improvement of dysphagia and odynophagia.', '\nOptical microscopy 20x showing basophilic filamentous bacteria (green arrows), compatible with actinomycosis\nFour months after the treatment started, the patient returned to the infectious disease outpatient clinic due to a new onset episode of moderate epigastric pain without fever or further symptoms.']","Fig. 3 Optical microscopy 20x showing basophilic filamentous bacteria (green arrows), compatible with actinomycosis",yes
PMC7360013,Figure_6,oa_package/fc/ef/PMC7360013.tar.gz,"[' 6) involving sclerosing adenosis.', 'Coarse magenta microcalcifications are easily identified and were likely the impetus for biopsyDiscussionCore needle biopsy interpretation is a reliable method for triage of mammographic abnormalities.']",Fig.6 Lobular neoplasia involving sclerosing adenosis. Notice nesting pattern of non-cohesive bland uniform cells distending the sclerosing adenosis and minimizing the hyalinized pink stroma. Coarse magenta microcalcifications are easily identified and were likely the impetus for biopsy,yes
PMC9707608,Figure_1,oa_package/00/2e/PMC9707608.tar.gz,['Case 1 imagesA: CT showing an esophageal heterogeneous mass that is 26.'],"Figure 1 Case 1 images A: CT showing an esophageal heterogeneous mass that is 26.9 mm thick in the distal third of the esophagus. B: CT showing innumerable solid hypodense hepatic masses (largest measuring 39.1 mm) with associated upper abdominal lymphadenopathy. C: EGD showing a large, non-obstructing, circumferential, ulcerating mass in the lower third of the esophagus. EGD:esophagogastroduodenoscopy",yes
PMC9514915,Figure_2,oa_package/1e/46/PMC9514915.tar.gz,"['The primitive gut divides the primitive mesentery into dorsal and ventral mesenteries (\n\n).', '8\n9\n\nDiagram transverse through 4-week embryo (inset focuses on splanchnic mesoderm) demonstrates serous membrane (\ngray arrows\n) outlining the coelomic cavity (\nlight green arrows\n) and primitive mesentery.']","Fig. 2 Diagram transverse through 4-week embryo (inset focuses on splanchnic mesoderm) demonstrates serous membrane ( ) outlining the coelomic cavity ( ) and primitive mesentery. Splanchnic mesoderm (brown arrows) has unfolded from the midline and formed the primitive mesentery, suspending and containing the primitive gut ( ). The primitive gut ( ) divides the primitive mesentery into dorsal ( ) and ventral ( ) mesenteries. The subserous space ( ) is a continuous space made up of the splanchnic mesoderm ( ) and somatic mesoderm ( ).",yes
PMC3202547,Figure_3,oa_package/45/b1/PMC3202547.tar.gz,"['Also, while retinal insulin levels after subconjunctival injections are supra-physiologic, they did not cause any increase in retinal cell death ().', 'g003Ocular insulin administration and serum glucose normalization can both block diabetes induced retinal cell death.', 'In the present study, subconjunctival administration of low-dose insulin partially prevented this increased cell death as demonstrated by both reduction in the number of TUNEL-positive retinal cells and DNA fragmentation in the treated rats when compared to the untreated diabetic animals (A and 3B).', 'Both subconjunctival administration of low-dose insulin and systemic administration of phloridzin reversed the increased retinal cell death observed in 4 weeks diabetic rats (B).', 'Interestingly, applications of both treatments reversed diabetes-induced retinal cell death to similar degrees (over 60 ) after longer duration of diabetes as demonstrated by the effect on both the number of TUNEL positive cells (C) and DNA fragmentation (D) in retina from 12 weeks diabetic animals.', 'We also showed that this exogenous insulin in the retina was biologically active, as indicated by the increase in phosphorylation on the serine 473 residue of Akt (), which is one of the kinases downstream of the insulin receptor that mediates neuroprotection The cat was hospitalised and an ambulatory ECG monitor was fitted for overnight ECG recording (Lifecard CF; Spacelabs Healthcare).']","Figure 1 Two-dimensional echocardiogram showing the right parasternal long-axis and short-axis views demonstrating normal atrial and ventricular size, wall thickness and mitral valve morphology. The left atrial:aortic ratio is within normal limits (1:1.3)",yes
PMC11401732,Figure_1,oa_package/47/86/PMC11401732.tar.gz,"['The frequency of PTC was significantly higher in N-GD () (p 0.', 'Graves disease is characterized by diffuse hyperplasia (A).']",Figure 1 Graves disease is characterized by diffuse hyperplasia BRAF- and RAS-like papillary thyroid carcinomas identified in Graves disease,yes
PMC7566418,Figure_3,oa_package/a8/5a/PMC7566418.tar.gz,['\nRT-QuIC analysis of ALS and FTLD-TDP CSF samples.'],"Figure 2 In brief, 20L of sonicated and diluted (10 ) BHs collected from three FTLD-TDP patients (BH*, purple lines) and one CTRL patient (CTRL BH, black line) were added to purified seed-free recombinant HuTDP-43 (1mg/mL) ( ) and HuTDP-43(263-414) (0.2mg/mL) ( ) and analysed by means of RT-QuIC. The reaction was exposed to 60s of shaking and 60s of rest. All positive BHs efficiently seeded the reaction, whereas the negative control BH at the same dilution (10 ) did not affect the aggregation kinetics of the reaction. ( ) Optimized protocol for BH analysis using purified seed-free HuTDP-43(263-414) (0.05mg/mL) as substrate with a different reaction buffer (no NaCl, Gdn-HCl added) and a modified protocol (shaking at 100rpm for 15s every 30min at 40C). ThT fluorescence intensity was plotted against time. Each graph has a specific range of ThT fluorescence values and duration of a given reaction. The experiment was performed in triplicate and each replica was performed three times. Curves represent means for all experimental replicates and bars indicate the standard deviations (SD).",yes
PMC7413444,Figure_5,oa_package/64/89/PMC7413444.tar.gz,['(b): Low power view showing intramembraneous ossification and bony spicules in dermis (H and E 100)Highpower view showing osteoblasts with eccentrically placed nuclei embedded in eosinophilic matrix (H and E 400)DiscussionDisorders of extraskeletal bone formation are classified as nonhereditary and hereditary conditions.'],Figure 5 Highpower view showing osteoblasts with eccentrically placed nuclei embedded in eosinophilic matrix (H and E 400),yes
PMC6621983,Figure_4,oa_package/01/a3/PMC6621983.tar.gz,"[' 4 shows measures from tasks assessing different aspects of learning and memory.', '05 versus Tg-SwDI 1 hMotor, exploratory, and temperament measuresSeveral of the behavior measures in ', '4).']","Fig. 4 Performance on cognitive tasks of learning and memory. Performance on the object displacement task, as measured by percentage of time spent with the displaced object. Performance on the novel object recognition task, as measured by percentage of time spent with the novel object. Performance on the Y-maze task, as measured by percent alternation between arms. Performance on the Barnes maze task by trial, as measured by latency to find the escape hole (max time=300s). Data points for each group were fit with a linear line to quantify the rate of learning. versus versus",yes
PMC4994244,Figure_1,oa_package/e8/57/PMC4994244.tar.gz,"[' 1a).', 'Table 1Antibodies (at 1 mg/ml) used for establishing AlphaLISA assays are listed below together with provider informationCloneCompanyCatalog #Host speciesClonalityEpitopeSpecific detection ofHumanRodentS129 phosC-20Santa Cruzsc-7011-RLrabbitpolyclonalC-terminusyesyesnoLB509CovanceSIG-39725mousemonoclonal115 122yesnonoasyn204Cell Signaling2647BFmousemonoclonal087 111yesnonoasyn211Abcamab80627mousemonoclonal121 125yesnono4B12CovanceSIG-39730mousemonoclonal103 108yesnonopSyn#64WAKO014-20281mousemonoclonal124 134yesyesyesEP1536YAbcamab173323rabbitmonoclonalyesyesyesMJF-R13Abcamab176839rabbitmonoclonalyesyesyes11A5ProthenaN/Amousemonoclonal124 134yesyesyesEvaluation of antibodies in relation to species specificity and S129 phosphorylation state.', 'For lysates 100 g of protein per lane and for recombinant h-asyn 2 ng of protein were loadedNext, we investigated the specificity of four antibodies to pS129 asyn and any reactivity to the non-phosphorylated variant.', ' 1b).', ' 1b).']","Fig. 1 Evaluation of antibodies in relation to species specificity and S129 phosphorylation state The species specificity of asyn antibodies used in the assay development (see Table ) was tested using Western blot analysis by loading three different lysates rat brain samples overexpressing h-asyn (AAV hasyn rat), uninjected wild-type rats (uninj. WT rat) or asyn knockout mouse brain tissue (asyn k.o. mouse) ( ). For lysates 50g of protein was loaded. Actin is shown as a loading control. The specificity of antibodies for pS129 state was assessed using recombinant pS129 h-asyn protein or S129A h-asyn protein. Two additional lanes were loaded with either rat brain lysate overexpressing h-asyn (AAV hasyn rat) or asyn knockout mouse brain lysate (asyn k.o. mouse) to evaluate unspecific binding of antibodies on actual samples ( ). For lysates 100g of protein per lane and for recombinant h-asyn 2ng of protein were loaded",yes
PMC2000912,Figure_2,oa_package/5f/e5/PMC2000912.tar.gz,['Binding of anti-CD8 mAb to monocytes is independent of FcR.'],"Figure 2 Binding of anti-CD8 mAb to monocytes is independent of FcR. , Blockade of Ig binding to CD64 with anti-CD64 mAb decreases binding of isotype mAb but not anti-CD8 mAb to monocytes. Bracketed numbers are geometric means of indicated peaks. , Western blot with anti-CD8 D9 detects a 32 kDa protein as expected for CD8 in THP-1, peripheral blood lymphocytes, thymus lysate, peripheral blood monocytes (>99%), GM-CSF differentiated M, PBMC, thymus, and a CTL clone but not in the lung epithelial line A549 (CD8 negative control). Right, anti-CD8 mAb B9.11 detects a 32 kDa protein as expected for CD8 in THP-1 and peripheral blood lymphocytes. 11.5 10 cell equivalents were loaded in each lane.",yes
PMC4371682,Figure_6,oa_package/e3/90/PMC4371682.tar.gz,"['After anesthetic block, the avulsion of the\nnail plate is performed and one can visualize the tumor projections, which will be removed\nwith scissors or scalpel ().']",FIGURE 6 Intra-operatory . Onychomatri coma with tumoral projections seen after the avulsionof the nail plate (illustrative image of another clinical case),yes
PMC5608837,Figure_4,oa_package/63/39/PMC5608837.tar.gz,"['4 and 5).', 'On autopsy an adhesion ileus was confirmed\nAxial postmortem CT in a boy age 4 years 4 months shows an ileocolic intussusception (arrow).']",Fig. 4 Postmortem CT in a girl age 6years 11months with an adhesion ileus. Coronal reconstruction shows the presence of collapsed small-bowel loops in the right upper abdomen ( ). On autopsy an adhesion ileus was confirmed,yes
PMC10564263,Figure_2,oa_package/88/a8/PMC10564263.tar.gz,"['CT - Mesenteric aneurysms10 mm fusiform aneurysm of the jejunal branch of the superior mesenteric artery, and 9 mm distal superior mesenteric artery aneurysm with further small areas of focal vascular ectasia of the ileal branches (arrows)The patient was treated for pericarditis with colchicine and ibuprofen, anticoagulated for atrial fibrillation with rivaroxaban (initiated one-week post-discharge), and rate-controlled with bisoprolol.']","Figure 2 CT - Mesenteric aneurysms 10 mm fusiform aneurysm of the jejunal branch of the superior mesenteric artery, and 9 mm distal superior mesenteric artery aneurysm with further small areas of focal vascular ectasia of the ileal branches (arrows)",yes
PMC3846645,Figure_1,oa_package/f5/fb/PMC3846645.tar.gz,['Evaluation of accuracy of fine needle aspiration cytology and histopathology for diagnosis of feline mammary tumours.'],"Figure 1 : Malignant multinucleated mammary epithelial cell; nuclei exhibit nuclear criteria of malignancy; nuclei super imposed and in different focal planes. May-Grunwald-Giemsa staining method, 1000X. : Cells exhibit variable numbers of mitoses are found; or the second populationof cells may have oval to fusiform vesicular nuclei with an extensive amount of eosinophilic cytoplasm and distinc cell margins (H&E, X 400). : Solid adenocarcinoma; The presence of neoplastic, mitotic and inflammatory cells (H&B, X 200.), : Hemorrhage foci and the central necrotic areas are interpreted as an indication that the neoplastic cells are growing faster and that there is therefore a higher risk of progressionto invasive carcinoma (H&E, X100).",yes
PMC9441750,Figure_4,oa_package/13/01/PMC9441750.tar.gz,"['No areas of LGE (defined as hyperintense areas after administration of gadoteric acid 6 standard deviations than a region of interest in the background) were detected in the myocardium, covering the entire left ventricle from the mitral valve plane to the apex ().', 'Late gadolinium enhancement (LGE) images without hyperintense myocardial areas at the left ventricle.']",Figure 4 Late gadolinium enhancement (LGE) images without hyperintense myocardial areas at the left ventricle.,yes
PMC9576154,Figure_8,oa_package/0e/d5/PMC9576154.tar.gz,[],"Figure7 After sorting and mRNA extraction, (mouse TDP-43) and mRNAs levels were measured in the sorted control cells (CTRL, not transfected GFP negative) and siTDP-43 transfected (GFP positive) cells. ( ) Quantitative real-time PCR analysis showed a significant decrease of the , and (coding for GluN2B) mRNAs in transfected cells compared to control cells (CTRL), while the mRNA expression of , (coding for GluN1), and (GluN2A) did not change. ( ) The mRNA expression of Synapsin-3 ( ), (coding for PSD93), (PSD95) and was also significantly decreased. Synapsin-1 ( ) and -2 ( ), Carboxypeptidase E ( ), Scribble component 2 ( ), Syntaxin-1 ( ) and SAP97 ( ) mRNAs did not change. Values were normalized to the levels of the housekeeping gene from four independent experiments. Error bars indicate mean SEM. Statistically significantly differences are indicated as follows: * < 0.05; ** < 0.01 using the MannWhitney test.",yes
PMC8225964,Figure_2,oa_package/a6/39/PMC8225964.tar.gz,"['A skin incision was carried to expose the bony exostosis, which was then bluntly dissected until freed from the underlying tissues (, A-B).', 'Macroscopically, a fungiform 3.', 'Generally, the lesion appeared like a miniaturized endochondral ossification with the most matured bone located peripherally (, C D).']","Fig. 2 (A) Intraoperative images showing exposure of the bony mass; (B) mass after excision of the whole lesion; (C) Photomicrograph showing the essential components of the whole lesion; (D) The inset box in C is magnified. In the whole slide photomicrograph (C), from left to right, the skin appendages (S) overlying the fibrous cap (F) investing the lesion and the bony component (B) immediately subjacent. The deeper part of the lesion closer to the mandible comprised the cartilaginous part (C) covered by a thick scar-like fibrous tissue (F). Some of these features are better appreciated as magnified in (D). Scale bars C: 4mm; D: 600m.",yes
PMC3921764,Figure_9,oa_package/7f/3e/PMC3921764.tar.gz,['Ectopic sebaceous glands on the glans penis.'],Fig. 10 Typical melanocytic nevus on the shaft of the penis.,yes
PMC4276947,Figure_5,oa_package/0b/82/PMC4276947.tar.gz,"['Plaque size at different times post-exposure in the absence of bLF (A,B) and bLF effects of high titer plaque formation at 48 h p.', '(C) was investigated.', 'Immunofluorescence confocal microscopy of SINVTaV-GFP-TC infection of C7-10 cells monitored over time in the same culture plates.', 'When utilizing a TC-adapted TR339 variant (SINVTaV-GFP-TC) in mosquito cell plaque assays, virus propagation was temporally limited in the presence of bLF (Table 1; ).']","Figure 5 Immunofluorescence confocal microscopy of SINVTaV-GFP-TC infection of C7-10 cells monitored over time in the same culture plates. Composite images; immunofluorescent (left) compared with immunofluorescent merged with phase contrast (right) revealing background monolayer ( , ); Plaque growth in cells infected with a M.O.I. of 0.1 in the absence of bLF at ( ) 24 h p.i. and ( ) 48 h p.i.; ( ) Plaque growth in cells infected with a M.O.I. of 100 at 48 h p.i. in the absence (left-image) presence bLF (right-image).",yes
PMC7571513,Figure_6,oa_package/a1/72/PMC7571513.tar.gz,"['Compression-sided injuries occur at the inferomedial femoral neck and have low risk of displacement, and they can typically be managed conservatively ().', 'Compression type stress fracture at the medial aspect of the left femoral neck base in a 21-year-old runner.']",Figure 6 Compression type stress fracture at the medial aspect of the left femoral neck base in a 21-year-old runner. A) Coronal T1W magnetic resonance (MR) image demonstrating low T1 signal (arrow) in the medial femoral neck. B) Coronal short-tau inversion recovery. MR image demonstrating an incom- plete fracture line perpendicular to the cortex with surrounding high signal bone marrow oedema (arrow),yes
PMC11540025,Figure_2,oa_package/f7/7f/PMC11540025.tar.gz,"['Evans blue was injected into the lateral ventricle of the P3 wild type and mutant mice to monitor the CSF flow (', 'We observed Evans blue in all four ventricles and spinal cord subarachnoid spaces (SaS) within 10 minutes after injection in both wild type and mutant mice (', 'The mutant lateral ventricles appeared enlarged as expected on coronal vibratome sections (', '.', 'By tracing the injected fluorescent dye Evans blue, we compared multiple consecutive optic nerve cross sections at about 400 m behind the globe (the optic nerve was myelinated at this region; see Figs.', ' 2A, 2D).', 'A significant increase of subarachnoid space in the optic nerve was found at the ages of P21 and P35 (see ', ' 2D), which was further confirmed by quantification (', 'Thus, we examined a subdomain of the ONH between the choroid and proximal myelin boundary by performing immunolabeling of myelin basic protein (MBP) and unphosphorylated neurofilament (upNFH; Figs.', ' 2F, 2H).', 'Delineation of the choroid boundary was aided by upNFH staining, which also outlined choroid vessels (besides the nerve neurofilament) probably due to the use of secondary anti-mouse Ig antibody (see Figs.', ' 2F, 2H).', 'We measured the average height (h) and width (w) of this ONH subdomain as parameters to determine the mutant ONH deformation (see Materials and Methods, and Figs.', ' 2F, 2H legends).', 'Quantitative analysis demonstrated that the height of the mutant ONH was significantly lowered, whereas the width slightly expanded (Figs.', ' 2G, 2I).']","Figure 2. ( ) Schematic illustration of the Evans blue injection into the mouse brain ventricles. ( , ), Cerebrospinal fluid (CSF) flow passages were not obstructed in P3 spinal cord and brain ventricles. in indicate the subarachnoid space (SaS) of the spinal cord. ( ) Note the enlarged lateral ventricles of the mutant brains. LV, lateral ventricle; 3rdV, third ventricle; Aq, aqueduct; 4thV, fourth ventricle. point to ventricles. ( ) Representative pictures of the SaS of optic nerve sheath stained with Evans blue ( ) at P14, P21, and P35. The represent areas of the subarachnoid space/cavity (SaS). Micrographs were taken from sections of the optic nerve at about 400 m posterior to the sclera. This distance was calculated by section thickness multiplied by numbers from the sclera position. ON, optic nerve. ( ) Statistical analysis of the SaS areas with Student's -test. Four consecutive sections from five optic nerves (total 20) of three mice for each wild type and the mutant genotype were counted for subarachnoid space areas. ns, nonsignificant; **, < 0.1; *** < 0.01. Error bars indicate mean SD. ( ) Immunostaining of upNFH ( ) and MBP ( ) of P35 longitudinal eye sections through the optic disc. The optic nerve between choroid (c) and MBP (m) proximal margin is defined as cmONH. ( ) Quantification of the height (h) and width (w) of the cmNFH. The cmNFH area is roughly a trapezoid, with the midline parallel to the upper and lower bases representing the width. There were six eyes from three mice. ( ) P42 longitudinal eye sections stained for upNFH ( ) and MBP ( ). ( ) Quantification of the height (h) and width (w) of the cmNFH. There were six eyes from three mice. Student's -test, *, < 0.5; **, < 0.1; ***, < 0.01. Error bars indicate mean SD.",yes
PMC9668375,Figure_2,oa_package/1f/02/PMC9668375.tar.gz,"['MRI coronal plane view showing the large mass with soft tissue and cystic componentsCT sagittal plane demonstrating the fetus with soft tissue and bony componentsFurthermore, a three-dimensional (3D) reconstruction of the bony skeleton was done, demonstrating the fetal skeletal bones and limbs present within the abdominal cavity of the infant, as seen in .']",Figure 2 MRI coronal plane view showing the large mass with soft tissue and cystic components,yes
PMC3698899,Figure_2,oa_package/8d/99/PMC3698899.tar.gz,"['[34] When AVM is extensive with involvement of bones and soft tissues, multiple sessions of embolization by transarterial route or direct puncture using liquid embolizing agents like onyx [ev3, Irvine, USA] or n-BCA (n-Butyl-2-Cynoacrylate) glue [Bbraun, Aesculap, Germany] would be required[5] [].', ' (A-F)Percutaneous embolization of Scalp AVM 34-year-old female patient with history of pulsatile swelling on her forehead.']","Figure 2 (A-F) Percutaneous embolization of Scalp AVM 34-year-old female patient with history of pulsatile swelling on her forehead. DSA images of Left ICA (A) and Left ECA (B) shows forehead AVM with feeders from the supraorbital branch of ophthalmic artery and branches from superficial temporal artery (STA). (C) Photograph showing percutaneous direct puncture of the AVM and glue embolization. (D, E) Radiograph shows glue cast (white arrow) conforming to the AVM. (F) Postemoblization angiogram shows no residual AVM. Courtesy: Dr Uday Limaye, INR Division, KEMH, Mumbai",yes
PMC4742117,Figure_7,oa_package/f2/f1/PMC4742117.tar.gz,['Cell marker induction shows antigen specificity in 30 month old APP/PS1 miceMHCII+ and CD8a+ Dendritic cells (DCs) in the aged mice were studied by flow cytometry (not shown) after antigen stimulation for 24 hours.'],"Figure 7 Cell marker induction shows antigen specificity in 30 month old APP/PS1 mice MHCII+ and CD8a+ Dendritic cells (DCs) in the aged mice were studied by flow cytometry (not shown) after antigen stimulation for 24 hours. There was no significant difference when looking at the percentage uptake of MHCII+ DCs in terms of the different peptides ., However, significant differences were found in , when looking at the differences in CD8a+ DCs between the mutant peptide and all over levels ( < 0.05). In , significant differences were also found in CD8a+, MHC-II+ double positive DCs when comparing the mutant peptide to all over levels ( < 0.05).",yes
PMC7683057,Figure_2,oa_package/be/cd/PMC7683057.tar.gz,['Technical Validation of CSF p217 + tau assays.'],"Fig. 2 Technical Validation of CSF p217+tau assays. A) Serial dilution of a pool of low tau human CSF was measured with both p217+tau assays; dilution corrected concentrations are shown. The minimum required dilution was established as 1:8. B) A panel of HC CSF samples ( =53) were measured with PT3PT82 assay at 1:8 dilution, revealing all signal in linear range (pink box=CSF signal intensity relative to standard curve range). C) An 8-point calibrant curve was prepared then measured with the PT3HT43 assay on three separate days, revealing good inter-test precision. D) An AD CSF pool was serially diluted then measured at two sites with the PT3HT43 assay, revealing good inter-site precision. E) An AD CSF pool was treated with indicated amounts of alkaline phosphatase then measured with both p217+tau assays and the t-tau assay revealing phosphorylation dependency of the p217+tau assays. F) An AD CSF pool was stored at 4C, 22C, or 37C for 1, 2, or 4h then measured with the PT3HT43 assay. In parallel, samples were freeze thawed 1 or 2 additional times and tested similarly. G) CSF was collected from 4 donors, aliquoted and stored at 70C and measured every three months. In all stability studies the PT3PT82 signal was preserved. AEB, raw signal in Simoa assay. S/N, signal/noise.",yes
PMC7873790,Figure_3,oa_package/55/f2/PMC7873790.tar.gz,['Imaging findings via gadolinium-enhanced magnetic resonance imaging and fluorodeoxyglucose positron emission tomography.'],"Figure 3 Imaging findings via gadolinium-enhanced magnetic resonance imaging and fluorodeoxyglucose positron emission tomography. ( ) Gadolinium-enhanced T1-weighted image revealed thickening of the vessel wall at the aortic arch and the abdominal aorta at the diaphragm ( ) Fluorodeoxyglucose positron emission tomography ( F-FDG PET) revealed FDG uptake in the aortic arch and the abdominal aorta, with a standardized uptake value (SUV) of 23 like that in the liver (arrow).",yes
PMC6886832,Figure_3,oa_package/5d/73/PMC6886832.tar.gz,"['Metabolites composition of P2 and P4We next compared P2 (LD) and P4 (MetS) contents of metabolites ().', 'The quantitative LC/MS-MS data (A) derived from six MetS and six LD independent runs show a significant decrease in MetS vs LD of: glucose-6-phosphate (G6P) (p = 0.', 'TLC analyses (B) of extracts from four MetS and four LD pigs consistently show more than 3-fold decrease in lactate (n = 4, p 0.', 'g003Key metabolic changes in the MetS heart.']",10.1371/journal.pone.0225857.g003,yes
PMC10475991,Figure_4,oa_package/2b/61/PMC10475991.tar.gz,[],"Figure4 GALNT14 expression in PCa samples with different Gleason score. The relative expression change (ratio) of GALNT14 in cryopreserved PCa samples with Gleason score less than (n=6) or starting from 8 (n=5) compared to adjacent normal tissue (n=11) were determined by qRT-PCR. Expression in normal tissue is shown as distribution around the mean. GAPDH and HPRT1 both served as reference genes. GALNT14 expression is repressed on average in PCa regardless of the Gleason score. This repression is also supported by the GEPIA Database (adjusted graph). GALNT14 expression was assessed using tumor microarrays with 203 PCa specimens with different Gleason scores. Two representative samples per Gleason score are shown. GALNT14 is detected particularly in tumor cells with mostly weak (upper row) or moderate (lower row) intensity. Here, no direct correlation with Gleason score could be detected. Few samples (n=9) show an intense GALNT14 expression. Gleason score ranged from 8 to 9. Staining is concentrated on tumor cells and is localized near the nucleus. Scale bar, 200 m.",yes
PMC6900860,Figure_4,oa_package/b8/0c/PMC6900860.tar.gz,"['Computer-based reconstructions can generate visual impressions called tractography, () and from a mathematical perspective, the diffusion tensor is generally summarized in 2 parameters: mean diffusivity (MD) representing the rate of random molecular diffusion (lower values correspond to low diffusivity), and fractional anisotropy (FA) as a normalized measure of the isotropic character of the diffusion (0 = isotropic; 1 = anisotropic).', 'Examples of white matter tract orientation on processed DTI.', 'DTI: diffusion tensor imaging, FA: fractional anisotropyIn a 2 year follow-up study of 37 CIS patients, Rocca and colleagues observed a distributed pattern of increased white matter diffusivity at baseline (2-60 days after diagnosis), which remained stable for one year but resulted in a further increase at 24 months.']","Fig. 4 Examples of white matter tract orientation on processed DTI. Color denotes the direction of the first eigenvector at each voxel, weighted with the FA of that voxel. Red: left-right; blue: superior-inferior; green: anterior-posterior. Left: sagittal plane; middle: coronal plane; right: axial plane. DTI: diffusion tensor imaging, FA: fractional anisotropy",yes
PMC8866113,Figure_4,oa_package/99/88/PMC8866113.tar.gz,"[' 4a).', ' 4b, c).', ' 4a c).', ' 4d and e).', ' 4d, e).', 'Localization of VIM + cells expressing homeostatic and inflammatory markers reveals functional changes in EoE.', 'LIGHT-modulated inflammatory and homeostatic functions in esophageal fibroblasts are imprinted in EoEThe global transcriptome dysregulated in esophageal biopsies from active EoE patients compared to normal esophagus has previously been published30.']","Fig. 4 Localization of VIM+cells expressing homeostatic and inflammatory markers reveals functional changes in EoE. Representative images of normal ( =3) and active EoE esophagus ( =4) hybridized with specific probes for VIM (yellow) and WNT2B (green, LP=lamina propria, EPI=epithelium). Immunofluorescencestaining of normal ( =3) and active EoE esophagus ( =4) for VIM (green) and WNT2B (red). Quantification of VIM+WNT2B+cells in LP and EPI comparing normal and active EoE esophagus. Representative images of normal ( =3) and active EoE esophagus ( =4) hybridized with specific probes for VIM (yellow), ICAM-1 (green) and IL-34 (red). Quantification of VIM+ICAM-1+, VIM+IL-34+ and VIM+ICAM-1+IL-34+ cells in LP and EPI comparing normal and active EoE esophagus. White arrows in cpoint positive cells. * <0.05, ** <0.01 and *** <0.001.",yes
PMC4387147,Figure_3,oa_package/8e/e6/PMC4387147.tar.gz,['.'],"Fig. 3. Intraoperative and postoperative views. A, The design of the tumor excision. The resection margins were set below the eyebrow for the upper eyelid, at the orbital border for the lower eyelid, and 2 cm from the orbital border for the lateral margin. B, Harvested free forearm flap. We partially resected the middle section of the flap into a wedge shape to make it fit into the orbital floor. The flap was transferred by anastomosing the pedicle to the superficial temporal artery and vein. C, Five years after the operation. He has been wearing the epithesis for more than 5 years without any problem.",yes
PMC8590307,Figure_3,oa_package/39/33/PMC8590307.tar.gz,"[' 3).', 'The microscopy images (magnification 20) were acquired with the Nikon DS Camera Head DS-Fi1, DS Camera Control Unit DS-L2, and Nikon Eclipse E1000 Microscope at a resolution of 2560 1920 pixelsHe received 600 mg of rifampicin once per month and 75 mg of dapsone daily for 12 months at the diagnosis of the second relapse of leprosy.']","Fig. 3 Pathological findings of a skin biopsy. Superficial and deep perineural and perivascular dermatitis, suggestive of early leprosy lesions (I to BT). HE staining (left) and Fite staining (right, no bacilli seen) of a punch biopsy taken from the right thigh. Scale bars measure 50m. The microscopy images (magnification20) were acquired with the Nikon DS Camera Head DS-Fi1, DS Camera Control Unit DS-L2, and Nikon Eclipse E1000 Microscope at a resolution of 25601920 pixels",yes
PMC8597880,Figure_1,oa_package/91/38/PMC8597880.tar.gz,"['82 resolution (, and Supplementary Table 1).', 'NIH-CoVnb-112 binds to the RBD through a canonical site of vulnerability that significantly overlaps the ACE2 binding site (a b).', 'Over 70% of the contact surface is mediated through residues within the elongated tyrosine-rich Complementarity-Determining Region 3 (CDR3) that forms an extensive shape-complementarity interaction with the receptor binding motif (RBM, residues 403 505) of the RBD (c e).', 'The 21 amino acid length CDR3 contains six conserved tyrosines; of these, three pairs of - interactions appear to be crucial for the CDR3 loop conformation, including the face-to-face stacking of F490RBD and Y100Enb112 and the two face-to-edge contacts between Y449RBD-Y99nb112 and Y505RBD-Y102nb112, as documented by their high buried surface area (BSA) values (d).', 'Outside of the CDR3 s extensive contact region, the extended CDR2 hairpin also helps stabilize the RBD tip (residues 481 487) (c d).', 'As a result, F486RBD has the highest BSA value among all the RBD epitope residues (d).', 'On the other hand, the hydrogen-bond network between NIH-CoVnb-112 CDR3 residues and the RBD (residues 487 505) which ACE2 also uses in binding to the RBD bypasses the frequently mutated E484RBD (c).', ', MR-17,21 WNb-2,13 and VHH-E10\nf).', '32075877.', 'The nanobody and prototype RBD are colored as in .', 'The CDR sequences are colored as indicated in a.']","Figure 1. Molecular basis of SARS-CoV-2 spike recognition by NIH-CoVnb-112. ( ) Crystal structure of NIH-CoVnb-112:SARS-CoV-2 RBD complex. The framework of NIH-CoVnb-112 is shown as yellow ribbons with the CDR1, CDR2, CDR3 colored green, orange and pink, respectively. The SARS-CoV-2 RBD is colored blue. ( ) Comparison of the RBD-contact footprint of NIH-CoVnb-112 and ACE2. The contact surface area is indicated by yellow and green lines for NIH-CoVnb-112 and ACE2, respectively, over the molecular surface of RBD. The electrostatic potential is displayed over the RBD surface and colored for negative (red), positive (blue), and neutral (white) electrostatic potential, respectively. ( ) Enlarged view into the NIH-CoVnb-112:RBD interface. NIH-CoVnb-112 is shown as a ribbon with the same color scheme as ( ) and the SARS-CoV-2 RBD is displayed as an electrostatic potential surface. ( ) The interaction network at the NIH-CoVnb-112:RBD interface. The individual nanobody:RBD contacts are shown as lines with the diagram of buried surface area for individual interface residue shown on the sides. Nanobody residues in the framework and CDRs are colored as in ( ) with interactions defined by a 5- distance criterion cutoff shown as lines. The Kabat scheme was used to number nanobody amino acid residues, with unique insertion residues indicated by letter (e.g., 52a, 52b, 52c). Salt bridges and H-bonds (bond length less than 3.5 ) are shown as black and blue dashed lines, respectively. The ACE2 contact residues within the nanobody epitope are highlighted in red or otherwise in blue. The recurrently mutated RBD residues in SARS-CoV-2 variants are marked with black boxes. ( ) Orthogonal views of the interaction network between NIH-CoVnb-112 and RBD. CDR residues are colored as in ( ) and the RBD residues are colored in blue. Hydrogen bonds and salt bridges are denoted as yellow and black broken lines, respectively. ( ) Comparison of the mode of RBD recognition by NIH-CoVnb-112 to other RBD-specific nanobodies. The NIH-CoVnb-112-RBD complex was superimposed (based on the RBD) to five published RBD complex structures of RBD-specific nanobodies, including WNb-2 (PDB: 7LDJ), VHH-E (7KN5), MR17 (7CAN), Nb12 (7MY3), and VHH-U (7KN5). The SARS-CoV-2 RBD is shown in a semi-transparent blue surface with the receptor-binding motif (RBM) highlighted in red. Other nanobodies are shown with colored lines as labeled.",yes
PMC10454239,Figure_2,oa_package/55/25/PMC10454239.tar.gz,"['However, the levels of M2-related genes were indeterminate (A,B).', 'Further, we intersected the DEGs with macrophage-related genes (MRGs) in the GEO database and obtained 153 differentially expressed genes related to M (DEMRGs) (C).', ', cytokine receptor binding, cytokine activity, growth factor receptor binding, cytokine binding and chemokine activity) (D).', 'The KEGG analysis also revealed a marked induction of inflammation and cytokine pathways, including the cytokine cytokine receptor interaction, NF- B signaling pathway, IL-17 signaling pathway, TNF signaling pathway, Toll-like receptor signaling pathway and inflammatory bowel disease (E).', 'IL-21/IL-21R are involved in the M1 polarization and inflammatory responses of M during C.']","Figure 2 IL-21/IL-21R are involved in the M1 polarization and inflammatory responses of M during respiratory infection as determined by RNA sequencing (RNA-seq). RNA-seq analysis of gene expression profile was performed on lung tissues extracted from WT ( = 3) and il21r knockout (il21r KO) ( = 3) mice on day 7 of respiratory infection. ( , ) Heatmap displaying the relative expression levels of M1-related genes ( ) and M2-related genes ( ) in WT and il21r KO mice. Gene expression values are indicated by the color intensities of red (upregulated) or blue (downregulated). ( ) Venn diagram showing the overlap between the gene set of differentially expressed genes (DEGs) and the gene set macrophage-related genes (MRGs). The numbers shown in the diagram represent the number of genes in each group. ( ) GO analysis using differentially expressed genes related to M (DEMRGs) showing the top 15 GO terms. ( ) KEGG pathway analysis using DEMRGs showing the top 20 enrichment pathways. Data are presented as means SD from = 3 animals per group.",yes
PMC11506460,Figure_6,oa_package/7c/e2/PMC11506460.tar.gz,"['Type 3B failures in allografts involve fractures through the allograft, analogous to type 3A (implant breakage) fractures in endoprostheses ().', 'Imaging of an 18-year-old male, with history of high-grade osteosarcoma of the proximal humerus diagnosed at age 14 who received neoadjuvant chemotherapy, wide resection and osteoarticular allograft reconstruction, and revision surgery for nonunion 2 years after initial reconstruction (type 2B failure), now presenting 4 years after initial reconstruction with functional pain, found to have allograft fracture (type 3B failure).']","Figure 6 Imaging of an 18-year-old male, with history of high-grade osteosarcoma of the proximal humerus diagnosed at age 14 who received neoadjuvant chemotherapy, wide resection and osteoarticular allograft reconstruction, and revision surgery for nonunion 2 years after initial reconstruction (type 2B failure), now presenting 4 years after initial reconstruction with functional pain, found to have allograft fracture (type 3B failure). Initial ( ) radiograph and ( , ) MR images of the tumor at diagnosis. ( ) Radiograph demonstrating initial proximal humerus allograft reconstruction after tumor resection. ( ) Radiograph showing allograft fracture. ( ) Post-operative radiograph after revision to alloprosthetic hemiarthroplasty.",yes
PMC9825990,Figure_3,oa_package/14/a2/PMC9825990.tar.gz,['Distribution of driver mutations in HP exposed and HP na ve DGCs.'],"Figure 3 Distribution of driver mutations in HPexposed and HPnave DGCs. (A) Oncoprint of driver mutations (SNVs/indels). (B) Oncoprint of significant copy number alterations detected by GISTIC in the cytoband level. The analysis detected only the regions with amplification (copy number [CN]4) and not those with homozygous deletion (CN=0). Copy number gain (CN=3) or loss (CN=1) was not regarded as functionally significant in this analysis. (C) Oncoprint of fusion. Sample labeling for HP infection status, preservation type (FF or FFPE), TCGA molecular subtype, and eradication history is shown above the Oncoprints. FF, freshfrozen; FFPE, formalinfixed paraffinembedded; TCGA; the Cancer Genome Atlas.",yes
PMC7428785,Figure_4,oa_package/cb/a8/PMC7428785.tar.gz,"['Percent change of weekly imaging volume by modality type at (a) main hospital campus and (b) affiliated imaging centers, from January 2020 through May 2020.', 'DiscussionThe COVID-19 pandemic has had sweeping effects on the US healthcare system (8,9).']","Fig. 4 Percent change of weekly imaging volume by modality type at (a) main hospital campus and (b) affiliated imaging centers, from January 2020 through May 2020. Gray zone marks the significant decline in weekly imaging volume during the transition period, the time between the pre-SOE and post-SOE periods. Average weekly imaging volume of weeks 19 (pre-SOE period) was used as reference and considered 100%. The percentage listed beside each imaging modality represent the percent of baseline volume recovery at week 19.",yes
PMC9706584,Figure_5,oa_package/f8/d9/PMC9706584.tar.gz,"['Furthermore, microglial activation markers such as iNOS and CD86 were increased in A -treated primary microglia (D and Supplementary ).', ' A -specific Tregs suppress A -induced microglial inflammation via the bystander effect.']","Figure 5 (A) Representative images show the primary microglial activity after 30 min of A (5 M) treatment in the presence of CDr20. The scale bar represents 100 m. (B) Fluorescence intensity of fifty randomly selected cocultured microglia and Treg cells after 30 min of A treatment with CDr20 in the red fluorescent channel (excitation: 570 nm, emission: 600 nm). (C) The secreted levels of TNF- and IL-1 in the supernatants of cocultured cells treated with A for 6 h were measured by ELISA. (D) The relative mRNA expression levels of iNOS and CD86 in cocultured microglia and Treg cells were quantified. (E) Schematic diagram for the experiment using indirect coculture with a Transwell insert (0.4 m) to confirm the inhibitory effect of A Tregs. The relative mRNA expression levels of (F) the proinflammatory cytokines IL-1 and TNF- and (G) the microglial activation markers CD86 and iNOS were quantified in activated microglia after 6 hrs of coculture. (H) Schematic diagram for the A Treg migration assay. (I) The bar graph shows the percentage of Tregs that migrated toward A compared to KLH. (J) Schematic diagram of the migration assay performed with A Tregs and anti-CD40 or anti-CD80 antibody-treated primary microglia. (K) The bar graph shows the percentage of Tregs that migrated toward antibody-treated microglia. Data are presented as the mean SEM. n = 3-4 per group; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.",yes
PMC8522888,Figure_1,oa_package/f2/04/PMC8522888.tar.gz,"['Currently, the first prenatal examination of fetuses is possible around the 12th week of pregnancy () (Earlier scans at 4-6-8 weeks are performed only to confirm the presence of pregnancy, its location and whether it is single or multiple).', '270279_fig_001"" fig-type=""figure"" orientation=""portrait"" position=""float"">Fetal body in 3D ultrasound at the 12th week of gestation one of the very first medical diagnostic imaging examinations.']",Fig. 1 Fetal body in 3D ultrasound at the 12th week of gestation one of the very first medical diagnostic imaging examinations. Ryc. 1. Zarysy caego podu w badaniu USG w prezentacji 3D w 12 tyg. ciy jedno z pierwszych medycznych bada diagnostycznych.,yes
PMC9160407,Figure_7,oa_package/ce/a0/PMC9160407.tar.gz,['DiscussionVAPs are detected in 0.'],Fig. 7 Coronal contrast-enhanced CT. Reduction of the pancreatic duct dilation (arrow).,yes
PMC6766113,Figure_4,oa_package/81/c5/PMC6766113.tar.gz,"['003""/>The spleen after the inevitable spleen-sparing operation.']",Figure 4 The spleen after the inevitable spleen-sparing operation.,yes
PMC11090277,Figure_8,oa_package/1e/f7/PMC11090277.tar.gz,"['14 mm () and 8.', 'Median height pre-postoperative evolution by group.']",Figure 6 Postoperative CBCT measurement of the distal bone height of the edentulous site using xenograft materials.,yes
PMC11471303,Figure_5,oa_package/cb/70/PMC11471303.tar.gz,['Bedside retrograde urethrography (RGU) filmThe red arrow marks the site of the bulbar urethral stricture.'],Figure 5 Bedside retrograde urethrography (RGU) film The red arrow marks the site of the bulbar urethral stricture.,yes
PMC3800604,Figure_1,oa_package/4d/78/PMC3800604.tar.gz,"['Upexpression of OX42 and COX2 Induced by LPS in Substantia Nigra and StriatumIncreased Ox42 and COX2 expression began to be detected at 24 h after LPS challenge in both the substantia nigra and striatum (s 1(a) and 1(b)).', '0-5394911333118778772Microglia were responsing to LPS application.']","Figure 1 Microglia were responsing to LPS application. (a) Microglia have been significantly activated by increased levels of Ox42 in the striatum, as well as in the SN during one to three days. At the same time, the expression of COX2 increased as time passed. After mice were treated with LPS, SN and striatum were separated. The expressions of Ox42 and COX2 level were detected by Western blot analysis. LPS can cause neuroinflammation after intranigral injection. (b) Quantitative analysis of Ox42 and COX2 expression in SN and striatum during one to three days. Mean SD. ( = 3, ** < 0.01, *** < 0.001 versus saline group). (c) The expression of Ox42 was examined by immunohistochemistry. Coronal sections of SN; immunohistochemistry staining with Ox42 antibody to visualize microglia at 1d and 2d after unilateral application of LPS to the pial surface or application saline on the control side.",yes
PMC3705447,Figure_2,oa_package/1d/28/PMC3705447.tar.gz,"['By Western blotting, we confirmed 80% knockdown of ASC in the shASC cells compared to the shNeg cells (', ' gondii resulted in similar infection efficiencies (', ' gondii infection, we observed a 72% reduction in the amount of IL-1 released into the supernatant of the infected shASC cells compared to that of the infected shNeg cells (', 'CD11c was similarly upregulated on the surface of the shASC and shNeg cells after PMA stimulation (', 'FIG 2 T.']","FIG2 induction of IL-1 in shASC cells. THP-1 cells stably transduced with a nontargeting shRNA control (shNeg) or an shRNA targeting ASC (shASC) were mock infected or infected with GFP-expressing type II . (A) The degree of ASC knockdown was visualized by Western blotting. (B) At 24hpi, the percentage of GFP (infected) cells in the culture was measured by flow cytometry. (C) IL-1 released into the supernatant upon infection was measured by ELISA. No cells indicates samples in which parasites were added to wells without monocytes and were cultured in parallel as a negative control. n.d., not detected. Error bars represent the standard deviations of biological triplicates. (D) shNeg or shASC cells were either left unstimulated (gray histograms) or were stimulated (black histograms) with 0.5M PMA for 24h. The cells were stained with a control Ig (dashed histograms) or anti-CD11c antibodies (solid histograms) and examined by flow cytometry. These experiments were performed 4 (A to C) or 3 (D) times, and representative experiments are shown. Data shown in panels B and C are from the same experiment. ***, < 0.001 (Students test).",yes
PMC3846913,Figure_4,oa_package/92/7d/PMC3846913.tar.gz,['Successful re-ballooning of Type-1 endoleak.'],"Figure 4 Intraoperative closure of an endoleak type I , immediate therapy by reballooning , postinterventional result (Patient #5).",yes
PMC9862556,Figure_2,oa_package/72/f0/PMC9862556.tar.gz,"[' 2).', 'Kaplan Meier survival curves between groups.', 'a, c, and e are the original population, PSM population and IPTW population of the MIMIC database; b, d, and f are the original population, PSM population and IPTW population of the eICU-CRD database, respectivelyTable 2Analysis of the associations between all-cause in-hospital mortality and groupsNon cancerCancerHR(95%CI)HR(95%CI)p-valueMIMIC-IV Multivariate ModelReference1.', ' 2).', ' 2).']","Fig. 2 KaplanMeier survival curves between groups. , , and are the original population, PSM population and IPTW population of the MIMIC database; , , and are the original population, PSM population and IPTW population of the eICU-CRD database, respectively",yes
PMC4037939,Figure_6,oa_package/c4/a8/PMC4037939.tar.gz,"['Results of negative control antibody are shown in .', 'b) Elivision Super/HRP immunohistochemistry, 100Negative control antibody (a) normal, Elivision Super/HRP immunohistochemistry, 400.']","Figure 6 Negative control antibody (a) normal, Elivision Super/HRP immunohistochemistry, 400. (b) psoriasis vulgaris, Elivision Super/HRP immunohistochemistry, 200. c) localized pustular psoriasis, Elivision Super/HRP immunohistochemistry, 100. d) erythrodermic psoriasis, Elivision Super/HRP immunohistochemistry, 100",yes
PMC5524677,Figure_4,oa_package/02/2b/PMC5524677.tar.gz,"['IBA-1 immunostained dorsal CA1 hippocampal region of vanadium exposed (B,E,H), age matched control (A,D,G) and withdrawal group (C,F,I) mice after intermittent vanadium treatments for 6, 12 and 18 months revealed microglial activation identified by an enlarged cell body with several short, thickened processes, relative to the matched controls with longer, finer branches while the withdrawal groups showed better morphology relative to vanadium exposed groups.']","Figure 4 IBA-1 immunostained dorsal CA1 hippocampal region of vanadium exposed , age matched control and withdrawal group mice after intermittent vanadium treatments for 6, 12 and 18 months revealed microglial activation identified by an enlarged cell body with several short, thickened processes, relative to the matched controls with longer, finer branches while the withdrawal groups showed better morphology relative to vanadium exposed groups. Figure , the number of IBA-1 positive cells in hippocampal CA1 region, were significantly increased in all the vanadium exposed groups compared to control, while the withdrawal groups showed reversal effect. Microglia activation increases into advanced age with increasing vanadium exposure ( ; 6EB vs. 18EB = ** < 0.01), (ANOVA: * < 0.05, ** < 0.01). Magnification: 200 inset: 400.",yes
PMC4166351,Figure_1,oa_package/e8/dd/PMC4166351.tar.gz,['Schematic representation of A production and removal from the brain.'],"Figure 1 . The proteolytic processing of the large, transmembrane, APP occurs in two distinct amyloidogenic and non-amyloidogenic pathways. The amyloidogenic pathway involves the sequential cleavage of APP by an aspartic proteinase, -secretase, which releases a soluble ectodomain (sAPP) and the C-terminal fragment CTF99. This, in turn, is cleaved by another aspartic proteinase, -secretase, generating the transcriptional regulator APP intracellular domain (AICD), and releasing the 39-42 amino acid amyloid- peptide (A). Due to its very high ability for aggregation, A forms dimers, trimers and higher level oligomers which are toxic to cells and cause neuronal death. Formation of amyloid plaques from A aggregates in complex with other proteins is a hallmark of AD but is considered as a scavenging process. In the non-amyloidogenic pathway APP molecules are cleaved at the -secretase site within the A-domain releasing a soluble ectodomain sAPP and the C-terminal fragment CTF83. Proteolytic cleavage of CTF83 by -secretase releases AICD and p3 fragment whose functions are still unknown. The AICD fragment produced in the amyloidogenic pathway binds to a stabilizing factor Fe65 and in a complex with other factors (the histone acetyl transferase, Tip60, and a Mediator complex subunit Med12) can act as a transcription enhancer regulating expression of a variety of genes, including the A-degrading enzyme neprilysin and clearance protein transthyretin (TTR). This process was found to be specific to the neuronal APP isoform. AICD produced in the non-amyloidogenic pathway and from other APP isoforms (APP and APP ) is most likely degraded (by some intracellular proteases, e.g., insulin-degrading enzyme and caspases). Soluble APP ectodomains, sAPP and sAPP, have been shown to have neuroprotective properties.",yes
PMC3079085,Figure_3,oa_package/0f/30/PMC3079085.tar.gz,"['Color-flow Doppler echocardiography shows severe tricuspid valve regurgitation (A).', '5 mmHg (B).', 'The color-flow Doppler transthoracic echocardiography showed severe tricuspid regurgitation (A).']",Fig. 3 The color-flow Doppler transthoracic echocardiography showed severe tricuspid regurgitation (A). Peak velocity of tricuspid valve was 1.62 m/sec and right ventricular systolic pressure was 20.5 mmHg (B).,yes
PMC9746542,Figure_2,oa_package/8b/ea/PMC9746542.tar.gz,"['05, (b,c) than those corresponding to the APP/PS1 mice group.', '.']","Figure 2. Regulatory effects of Bacillus Calmette Guerin (BCG) treatment on dendritic morphology in the hippocampal CA1 area of APP/PS1 mice. (a) Representative image of a typical pyramidal cell used for analysis. The different compartments of the dendritic tree are indicated. (b) Histogram showing a comparison of the number of primary neurites in the hippocampal pyramidal cells, which increased following BCG treatment two and three times, relative to the number corresponding to the APP/PS1 mice; 1, 2, and 3 represents APP/PS1 mice injected with BCG once, twice, and three times, respectively. (ce) APP/PS1+3BCG mice showing significant increases in total dendritic length, apical dendritic length, and average dendritic length, respectively, compared with APP/PS1 mice. APP/PS1+2BCG showed an increased apical dendritic length at two weeks following treatment. Data were analyzed by performing Students -test, and the results obtained are presented as the meanSEM. =9 neurons (three neurons each from the three mice). * <.05 and ** <.01 vs. vehicle control.",yes
PMC11649523,Figure_5,oa_package/56/1f/PMC11649523.tar.gz,"['Kaplan Meier plots of nuclear count split at median within pT3 (A) and pT4 (B) melanoma subsets, showing that nuclear count has potential to refine T stage category.']","Figure 5 KaplanMeier plots of nuclear count split at median within pT3 ( ) and pT4 ( ) melanoma subsets, showing that nuclear count has potential to refine T stage category. [Color figure can be viewed at ]",yes
PMC5505051,Figure_2,oa_package/6f/9e/PMC5505051.tar.gz,['(a) DSA pre-embolization showing arteries to be embolized (yellow arrows); (b) Fluoroscopic image post-embolization and contrast wash-out showing VERB100 in arteries (red circle); (c) DSA post-embolization showing shadowing imaging artefact from the radiopaque beads in the arteries (red arrows).'],Figure 2 (a) DSA pre-embolization showing arteries to be embolized (yellow arrows); (b) Fluoroscopic image post-embolization and contrast wash-out showing VERB100 in arteries (red circle); (c) DSA post-embolization showing shadowing imaging artefact from the radiopaque beads in the arteries (red arrows). Catheter tip position is shown in the yellow circle and the blue arrow points to reflux of contrast in a more proximal arterial branch related to distal embolization.,yes
PMC11601602,Figure_1,oa_package/b1/84/PMC11601602.tar.gz,"['Brain MRI performed 14 months prior to the death showed bilateral putaminal atrophy more left than right (Extended data suppl-).', 'Neuropathology examination (see also Extended data suppl-) showed round and horseshoe-like NCIs visible in Hematoxylin and eosin staining in the hippocampus CA1 (', '1a), subiculum, granule cell layer of the dentate gyrus (b), and amygdala (', 'NCIs (cortex, hippocampus, amygdala, striatum, brainstem), neuronal nuclear inclusions (putamen), and typical Papp-Lantos bodies in the cortical, subcortical and cerebellar white matter and putamen were strongly immunoreactive for disease-associated -synuclein ( e o).', 'Asymmetric involvement was noted, including the amygdala (c, d and g, h) and frontal cortex (', 'The majority of -synuclein filaments isolated from this region comprised a doublet filament, which has a left-handed helical twist (Extended Data suppl-).', 'Detailed neuropathology report is provided in Extended data suppl-.', '32881101\n.']","Figure 1. Neuropathological findings. Round and horseshoe-like NCIs (indicated by black arrowheads) visible in Hematoxylin and eosin staining ( ) and immunostaining for disease-associated -synuclein ( ) in the hippocampus CA1 ( ), granule cell layer of the dentate gyrus ( ). Asymmetric involvement of the amygdala showing neuronal cytoplasmic inclusions and ballooned neurons in the left side ( ) and severe gliosis in the right side ( ) corresponding to differences in the load of -synuclein pathology in the left ( ) and right ( ). Immunostaining for disease-associated -synuclein reveals less neuronal cytoplasmic inclusions and thread-like pathology in the left ( ) than right ( ) frontal cortex and more Papp-Lantos bodies in the left ( ) than right ( ) cerebellar white matter. The putamen shows severe -synuclein pathology ( ) in the form of enlarged Papp-Lantos bodies ( ), neuronal cytoplasmic and nuclear inclusions ( ; arrow). Bar in figure ( ) represents 40 m for a, e, o; 20 m for b, f; 80 m for c, d; and m, 25 m for n; 200 m for g, h, i, j; and 120 m for k and l.",yes
PMC10331132,Figure_1,oa_package/0f/f8/PMC10331132.tar.gz,"['Planar image of lymphoscintigraphy amd multiplanar reconstruction of SPECT/CT.', 'The patient was managed conservatively with outpatient percutaneous and/or transvaginal catheter drainage once or twice weekly, and a low-fat diet with medium-chain triglycerides for 2 months.']","Fig. 1 Planar image of lymphoscintigraphy amd multiplanar reconstruction of SPECT/CT. (A) Ninety minutes after the injection, showing the reduced number of inguinal lymph nodes accumulating the isotope bilaterally and abnormal extranodal isotope distribution in the pelvis (black arrow). Fusion axial (B), coronal (C), and sagittal (D) SPECT/CT images of the pelvis demonstrate the extranodal hotspot in the pelvic cavity on the right side, 2 cm above the superior margin of the femoral head (thick white arrow). On axial imaging, another hotspot is observed in the midline pelvic cavity (narrow white arrow). SPECT/CT: Single-photon emission computerized tomography/computed tomography.",yes
PMC5353471,Figure_4,oa_package/d1/66/PMC5353471.tar.gz,"['A BAV was also confirmed on TEE with a severe aortic regurgitation and a torrential color jet that hit the mitral anterior leaflet aneurysm [, Videos 4 and 5].', 'mp4"" xlink:type=""simple"" id=""d35e182"" position=""anchor""/>Transesophageal echocardiography image demonstrating the anterior-posterior bicuspid aortic valve.']",Figure 4 Transesophageal echocardiography image demonstrating the anterior-posterior bicuspid aortic valve.,yes
PMC5528917,Figure_1,oa_package/40/26/PMC5528917.tar.gz,"['The biopsy was obtained ().', '.', '1177_2374289517711714-fig1""/>The low-powered Hematoxylin and Eosin (H E)-stained slide obtained from the biopsy contains the field in .', 'The image of is labeled showing the central papillary structure.']",Figure 1. Low-power H&E image from core biopsy of breast.,yes
PMC6150703,Figure_1,oa_package/03/b0/PMC6150703.tar.gz,"['\nMicroscopic aspects of rat molar root in axial or transverse section, revealing the root structures, including the pulp, alveolar bone and periodontal ligament (HE, 10X magnification).']","Figure 1 Microscopic aspects of rat molar root in axial or transverse section, revealing the root structures, including the pulp, alveolar bone and periodontal ligament (HE, 10X magnification).",yes
PMC11287926,Figure_3,oa_package/49/25/PMC11287926.tar.gz,"[' 3).', '01)Abbreviations PHG, Parahippocampal gyrus; DCG, Median cingulate and paracingulate gyri; PreCG, Precental gyrus; PoCG, Postcentral gyrus; IFGoperc, Inferior frontal gyrus, opercular part; CAU, Caudate nucleus; PUT, Lenticular nucleus, putamen; HES, Heschl gyrus; STG, Superior temporal gyrus; PAL, Lenticular nucleus, pallidum; NBS, network-based statistics; HM, high myopia; HC, health control\n\nGraph theory analysis of alterations in brain functional connectivity.', 'Abbreviations: PHG, Parahippocampal gyrus; DCG, Median cingulate and paracingulate gyri; PreCG, Precental gyrus; PoCG, Postcentral gyrus; IFGoperc, Inferior frontal gyrus, opercular part; CAU, Caudate nucleus; PUT, Lenticular nucleus, putamen; HES, Heschl gyrus; STG, Superior temporal gyrus; PAL, Lenticular nucleus, pallidum ; R, right; L, left\nDiscussionWe constructed a network matrix and used graph theory analysis to explore the topological attributes of abnormal resting-state brain functional networks in HM patients.']","Fig. 3 Graph theory analysis of alterations in brain functional connectivity. ( )Compared to the HC group, the increased brain functional connectivity in patients with HM. ( )Compared to HC group, patients with HM have reduced brain functional connectivity. Notes NBS method identified a significantly altered network (16 nodes and 19 connections) in HM group relative to HCs. ( <0.01). The thickness of the line represents the strength of the functional connection. Abbreviations: PHG, Parahippocampal gyrus; DCG, Median cingulate and paracingulate gyri; PreCG, Precental gyrus; PoCG, Postcentral gyrus; IFGoperc, Inferior frontal gyrus, opercular part; CAU, Caudate nucleus; PUT, Lenticular nucleus, putamen; HES, Heschl gyrus; STG, Superior temporal gyrus; PAL, Lenticular nucleus, pallidum ; R, right; L, left",yes
PMC5946518,Figure_6,oa_package/0f/e6/PMC5946518.tar.gz,"['Besides, CD44-positive cancer cells were mainly found at invasive front and at the periphery of tumor islands [].', 'Green arrows indicate vascular channels lined by CD44-positive tumor cellsHigh power of intermediate-grade sample indicates CD44 positivity in all tumor cells.']",Figure 6 High power of intermediate-grade sample indicates CD44 positivity in all tumor cells. Yellow arrow indicates the tumor-stroma interface,yes
PMC10853361,Figure_6,oa_package/00/40/PMC10853361.tar.gz,"[' 6 demonstrates the 3D functionality of the model, visualising a single case with multiple findings.', 'The findings predicted by the model are presented alongside the ground-truthDiscussionSince CT was invented there has been substantial improvement in spatial and contrast resolution, making it easier for clinicians to detect abnormalities.']",Fig. 6 A three-dimensional (3D) visualisation of a single case containing multiple clinical findings demonstrating the 3D functionality of the model. The findings predicted by the model are presented alongside the ground-truth,yes
PMC6521796,Figure_3,oa_package/a5/9f/PMC6521796.tar.gz,"['As shown in , overall IFN- expression was very low in spleens and slightly upregulated in WT but not IFNAR / spleens upon poly(I:C) treatment, whereas livers showed no IFN- expression.', 'IFNAR / livers and spleens show a comparable cytokine milieu upon poly(I:C) treatment.', 'Nevertheless, our data indicated that expression of IFN- , TGF- , IL-6, IL-10, IL-17, and IL-22 is not altered in the livers and spleens of poly(I:C)-treated WT and IFNAR / mice ().']","Figure 3 IFNAR livers and spleens show a comparable cytokine milieu upon poly(I:C) treatment. WT and IFNAR mice were injected with 15 g/g BW poly(I:C). Livers and spleens were isolated 10 hpi and organ homogenates were analyzed for expression of the indicated cytokines by ELISA. Organs of untreated mice served as controls ( = 36). Error bars indicate standard deviations. ns, not significant; 0.01 (unpaired two-tailed -test).",yes
PMC11270155,Figure_3,oa_package/48/a3/PMC11270155.tar.gz,['Axial view of a CT angiogram depicting in-stent restenosis of left internal carotid artery stent (arrow).'],Figure 3 Axial view of a CT angiogram depicting in-stent restenosis of left internal carotid artery stent (arrow).,yes
PMC4956632,Figure_4,oa_package/d4/4d/PMC4956632.tar.gz,"[' 4) [9].', 'Bone scintigram in a 25-year-old man with renal failure and arthralgia.', 'In addition, a prominent calvarium and faint visualization of the kidneys are also supportive of a diagnosis of renal osteodystrophyRugger jersey spineThis sign is pathognomonic for osteosclerosis in the thoracic and lumbar vertebrae associated with secondary hyperparathyroidism of chronic renal failure demonstrated in 27 % of patients on radiographs [11].']","Fig. 4 Bone scintigram in a 25-year-old man with renal failure and arthralgia. A frontal planar image ( ) and magnified view ( ) demonstrates expansion of the manubrium and sternum without manubriosternal joint expansion resulting in a necktie appearance. In addition, a prominent calvarium and faint visualization of the kidneys are also supportive of a diagnosis of renal osteodystrophy",yes
PMC11633090,Figure_1,oa_package/9a/1a/PMC11633090.tar.gz,"['For example, the covalent modification of HA with heavy chains (HCs) from the inter-alpha-inhibitor (I I) family of proteoglycans (A) is an understudied hallmark of tissue inflammation (3, 20 22), where the HC HA complexes formed (', 'Importantly, the HC HA composition (C) likely affects the adhesive and signaling properties of the HA matrix, particularly for immune cells (15, 16, 22).']","FIG 1 Putative roles of HCHA matrices in the SARS-CoV-2-infected lung. ( ) HA becomes covalently modified, in a TSG-6-dependent manner, with HCs from II proteoglycans to form HCHA complexes during inflammation (see reference )). ( ) Through a series of non-covalent HC-HC and HC-PTX3 interactions, HCHA and accessory proteins such as PTX3 are incorporated into a crosslinked HA matrix ( ). ( ) We hypothesize that HCHA-containing matrices will be found throughout the lung following SARS-CoV-2 infection, including in the airways, alveolar spaces, and interstitium. The exact composition and function, for example, in the different locations, remain to be determined. Figure created using .",yes
PMC4297388,Figure_6,oa_package/36/12/PMC4297388.tar.gz,"['However, whole mount analysis showed uniform LacZ and GFP staining throughout the mammary glands of 5 and 8 week old mice ( 6A-B).', 'Notably, LacZ or GFP staining of histological sections revealed that the progeny of Sox9 deleted precursors was restricted to the basal/myoepithelial layer with essentially complete absence from the luminal compartment ( 6C-D).', 'FACS analyses demonstrated that GFP+ progeny was present in both luminal and basal compartments in the control (MMTV-Cre; Sox9wt/wt; CAG-eGFP) mice ( 6E).', 'In contrast, the mammary glands of mice with MMTV-driven Sox9 deletion showed a near complete absence of GFP+ (sox9-deleted) progeny in the luminal compartment ( 6F), consistent with staining results ( 6C-D).', '\nSox9 deleted luminal cells are lost over time.', 'DiscussionSox9 has been established as a key regulator of tissue stem and progenitor cells during development in several organ systems [18-22,28], more recently has been linked to cancer stem cell function [24].', 'Second, when we carried out lineage tracing in the context of Sox9 deletion induced by MMTV-Cre, we noted that progeny of Sox9-deleted precursors was absent from the luminal epithelial compartment but such cells were still present within the myoepithelial/basal compartment and may represent the progeny of restricted myoepithelial progenitors that underwent Sox9 deletion ( 6).']","Figure 6 . LacZ staining of whole mount mammary glands from 5week old Sox9 cKO (R26R; MMTV-Cre; Sox9 ) mouse. . GFP in whole mount 8week old Sox9 cKO (CAG-EGFP; MMTV-Cre; Sox9 ) was visualized with fluorescence microscope. . Section of mammary glands from 5-week old Sox9 cKO (R26R; MMTV-Cre; Sox9 ) mouse, stained with X-gal. . Section of mammary glands from 8week old Sox9 cKO (CAG-EGFP; MMTV-Cre; Sox9 ) mouse, immuno-labeling with anti-GFP (green) and anti--SMA (red) antibodies. . CD24, GFP FACS analysis of cells isolated from control (CAG-EGFP;MMTV-Cre; Sox9 ) mouse mammary glands. . CD24, GFP Flow cytometry analysis of cells isolated from Sox9 deletion (MMTV-Cre; Sox9 ; CAG-EGFP) mouse mammary glands.",yes
PMC3621152,Figure_1,oa_package/29/e4/PMC3621152.tar.gz,"['After the preparation of tunica vaginalis flap from the left testis, the fibrotic plaque was excised, and the corporeal body was patched with the flap, using watertight absorbable sutures (s 1(c) and 1(d)).', '0-00225000863756415(a) Preoperative appearance of the penile curvature, (b) intraoperative appearance of the penile curvature, (c) importation of the flap of tunica vaginalis, (white arrow), (d) closure of curvature area, which was excised by the flap of tunica vaginalis, (white arrow) and (e) appearance of the penis at 3rd week after the operation.']","Figure 1 (a) Preoperative appearance of the penile curvature, (b) intraoperative appearance of the penile curvature, (c) importation of the flap of tunica vaginalis, (white arrow), (d) closure of curvature area, which was excised by the flap of tunica vaginalis, (white arrow) and (e) appearance of the penis at 3rd week after the operation.",yes
PMC6434764,Figure_4,oa_package/89/6b/PMC6434764.tar.gz,"['Lesions in 8 (66%) patients decreased spontaneously [] within 4 8 weeks period with placebo.', 'Regression of lesion in three patients at 4 weeksDiscussionLipodystrophy is a heterogeneous disorder characterized by atrophy and infrequently hypertrophy of adipose tissue.']",Figure 4 Regression of lesion in three patients at 4 weeks,yes
PMC3435948,Figure_11,oa_package/da/e2/PMC3435948.tar.gz,[],Figure 11. Sixteen-year-old boy. Coronal T2 fat saturated. Edema is seen at the ulnar collateral ligament insertion on the sublime tubercle. The periosteum (arrow) is elevated and partially stripped with bright signal (curved arrow) between it and the cortical surface of the ulna.,yes
PMC4028918,Figure_3,oa_package/7d/5a/PMC4028918.tar.gz,['(B) Axial image at the level of third thoracic vertebra showing the position of medial limb of LVA (arrow) between LCCA and the LSAVR images of right lateral and left lateral views showing the level of fusion (arrow) of the two limbs of the right and left vertebral arteries.'],Figure 3 VR images of right lateral and left lateral views showing the level of fusion (arrow) of the two limbs of the right and left vertebral arteries. The single trunk of RVA is entering C-4 transverse foramen and the LVA entering C-5 transverse foramen,yes
PMC8635056,Figure_2,oa_package/a9/58/PMC8635056.tar.gz,"['To further estimate the distribution of Ttyh1 in neurogenic niches, and track the progeny cells of Ttyh1+ NSCs, we constructed Ttyh1-CreERT2 transgenic mice (A), which were crossed with Ai9 Rosa26-tdTomato reporter mice to obtain double transgenic offspring contains Ttyh1-CreERT2; Rosa-tdTomato.', 'Five days after induction on 8 weeks old adult mice, the brain tissues of control Rosa-tdTomato mice did not show fluorescent signals (data not shown), but the brain tissues of Ttyh1-CreERT2; Rosa-tdTomato mice showed intensive red fluorescent signals (B).', 'Five (s 2B,C) and 10 days (C) after induction, Ttyh1+ cells were largely located in the striatal side in SVZ (arrowheads in B), especially in the dorsal wedge (arrows in B).', 'Importantly, Ttyh1+ signals were observed in the subgranular layer within the dentate gyrus (dotted lined area in B) of hippocampus.', '27% in SGZ, respectively (C), at 5 days post injection (5 dpi).', 'The cells co-labeled with Ttyh1 and DCX are mainly located in the dorsal wedge of SVZ (B), indicating that Ttyh1+ positive cells underwent neurogenesis process.', 'Indeed, Ttyh1-tdTomato and EGFR, DCX co-label in the SVZ zone respectively, but with low percentages (C), indicating that Ttyh1+ NSCs differentiate at a quite low frequency.', 'The results showed that Ttyh1 was mainly expressed in qNSCs (E).', 'Violin plot clearly showed the process of Ttyh1 decreasing with the differentiation of NSCs (F).', 'In addition, Ttyh1 was highly expressed in niche astrocytes (F).', 'Notably, we also observed Ttyh1+ cells outside the SVZ and SGZ region (B).']","FIGURE 1 Ttyh1 is expressed in Ki67 quiescent NSCs in SVZ. Double-labeling of Ttyh1 and NSC markers. Ttyh1 co-labeled cells with CD133, GFAP and Sox2 at high frequency in SVZ. Double-labeling of Ttyh1 and TAP or neuroblast cell markers. Ttyh1 seldomly labeled EGFR and DCX cells. Double-labeling of Ttyh1 and proliferative cell marker Ki67. Ttyh1 completely were not co-labeled with Ki67 in entire SVZ region. The non-overlapping of Ttyh1 and Ki67 signals are showed in magnifications within white rectangles (the lower field is the magnification of the dotted rectangle in upper field). Statistics of the proportions of co-labeled cells in Ttyh1 cells. Scale bar = 10m in A, Scale bar = 50m in B, Scale bar = 100m in C, Scale bar = 10m in D. = 3 for all experiments. Data are expressed as mean SEM. LV, lateral ventricle.",yes
PMC11273710,Figure_2,oa_package/7a/80/PMC11273710.tar.gz,"['Histological examination of the specimen showed typical features of schwannoma with Antoni A alternating with Antoni B areas (-2(A)).', 'The tumor cells were diffusely positive for S-100 (-2(B)) and SOX-10 (-2(C)) immunostains.', '.', '1177_20363613241267740-fig2"" position=""float""/>The patient was scheduled for follow-up 6 months after the shunt insertion and had an excellent uneventful recovery post intervention.']","Figure 2. Histopathological examination of the intraventricular tumor. A: There is proliferation of spindle-shaped cells arranged in nodules showing palisading of nuclei consistent with Antoni-A areas (Arrow), alternating with hypocellular areas consistent with Antoni-B. B: S-100 immunostain is positive in the tumor cells with nuclear and cytoplasmic staining. C: SOX-10 immunostain shows strong nuclear staining.",yes
PMC7102595,Figure_2,oa_package/64/5f/PMC7102595.tar.gz,['Chest CT scan of a 50-year-old Iranian man with confirmed diagnosis of COVID-19 pneumonia.'],"Fig 1 Chest CT scan from a 50-year-old male Chinese patient with a confirmed diagnosis of novel COVID-19-infected pneumonia. The patient presented with low-grade fever, cough, sneezing, fatigue, and lymphopenia. Multiple peripheral ground-glass opacities are present in both lungs (predominant on the right side), with a subpleural distribution. Imaging findings are nonspecific and might be seen with other viral pneumonias as well. Images are courtesy of Min Liu, MD, Department of Radiology, China-Japan Friendship Hospital (Beijing, China).",yes
PMC10644662,Figure_4,oa_package/47/37/PMC10644662.tar.gz,"[' 4a) with an established tau biosensor cell seeding assay.', ' 4b and c) [2].', ' 4d), confirming TTT as a robust readout for quantification of tau seeding activity in AD brains.', ' 4b and c), while 216-fold dilution largely led to a loss of signal (data not shown).', ' 4a) and was thus at least 4 more sensitive than cellular tau seeding.', 'Tau SAA TTT of AD brain homogenates from cohort 1 correlated with Tau biosensor cell seeding assay, phosphorylated Tau, and aggregated Tau.', 'Cohort 1 is described in detail in Table 1 and Additional file 1: Table S2The presence of aggregated and phosphorylated forms of tau is a pathological hallmark of AD brains [45].', ' 4e and f).', ' 4e and f) [46].', ' 4g and h) [14].', ' 4e, g).', ' 4i).', ' 4j), it did inversely correlate with ptau212/214 and aggregated tau (', ' 4f, h).', ' 4, 5 and Additional file 1: ']","Fig. 4 Tau SAA TTT of AD brain homogenates from cohort 1 correlated with Tau biosensor cell seeding assay, phosphorylated Tau, and aggregated Tau. Nineteen AD brains (red) and one control brain pool (CTR, blue) were analyzed with Tau SAA. Tau SAA was performed with brain homogenates diluted 10 . X-axis indicates case numbers and brain regions (EC: Entorhinal cortex; FC: Frontal Cortex; HIP: Hippocampus; TC: Temporal Cortex). Dots represent technical replicates. Bars and error bars indicate medians and interquartile ranges. Representative images from Tau biosensor cell seeding assay. HEK293 cells stably expressing Tau repeat domains (amino acids 244372) with P301S mutation fused to yellow fluorescent protein (Tau-YFP) were incubated with AD and CTR brain pools for 48h. Afterwards, cells were fixed, stained with DAPI and imaged for Tau-YFP (green) and DAPI (blue). Scale bar represents 15 m. Quantification of YFP-positive inclusions in Tau biosensor cell seeding assay incubated with brain homogenates diluted 1:8. Spearman correlation of Tau SAA TTT with percent of cells with inclusions in Tau biosensor cell seeding assay. Quantification of pTau212/214, aggregated Tau and total Tau by ELISA, and their Spearman correlation with Tau SAA TTT. Dotted lines show limits of detection (LOD). Cohort 1 is described in detail in Table and Additional file : Table S2",yes
PMC4947121,Figure_4,oa_package/60/48/PMC4947121.tar.gz,"[' 4a, blue arrows) and various-sized dense lipid-containing autophagic vacuoles (', ' 4a, red arrows) localized to perikarya, while age-matched vehicle-treated (nonTg-CT) (Suppl.', ' 4b, d i and Suppl.', ' 4i) was identical in AD-CT and AD-D6 mice, but the number of larger sized autophagic vesicles, many containing lipids ( 0.', ' 4i), was significantly increased in AD-D6 mice.', ' 4d, e i and Suppl.', ' 4i), probably corresponding to immature autophagic vesicles [62].', ' 4h, and Suppl.', ' 4d, g and Suppl.', ' 4d, yellow arrows), as compared to control mice (', ' 4c yellow arrows).', '', '001 indicate significant differences relative to AD-CT\nWe next compared the effect of D6-treatment on APP-CTF accumulation in 2xTgAD and 3xTgAD mice.']","Fig.4 In vivo treatment of 3xTgAD mice with the -secretase inhibitor ELND006 (D6) leads to autophagic pathology and intraneuronal damage. Electron microphotographs of neuronal somas and neuropil from 5-month-old 3xTgAD males treated with D6 (AD-D6) ( , , , ) or vehicle (AD-CT) ( , ) during 1month. Neuronal perikarya of AD-CT and AD-D6 mice contained dense lysosomal bodies ( ) ( , ) and lipid-containing autophagic vacuoles ( ) ( ), that are more abundant and enlarged in AD-D6 mice ( ) ( , , ). The AD-D6 mice also displayed multiple large vesicles filled with heterogenous material ( ) and multilamellar bodies ( , ML).The neuropil of AD-CT mice presented many normal appearing synaptic contacts characterized by typical synaptic post-densities (PSDs, in ) and normal mitochondria ( ). In contrast, the neuropil in AD-D6 mice displayed very few normal appearing synaptic contacts ( ), many damaged mitochondria ( , , ) and electron-lucent areas ( ). are 2m in , , , , 5m in , , , and 10m in . Quantification of autophagic structures in slices from AD-CT ( ) and AD-D6 ( ) mice. The autophagic structures (AVs) were classified in three (13) distinct groups, 1 corresponding to small dense AVs ( , less than 1m), 2 to a mix of larger sized and more or less lipid-containing AVs ( ) and 3 to large vesicles containing membranous material and multilamellar bodies ( ). correspond to the average number of AVs per neuron (per cross section) and a count of 4050 neurons per mouse (2 mice for each condition). Data are represented as meanSEM and statistical analysis was performed using the MannWhitney test and *** <0.001 indicate significant differences relative to AD-CT",yes
PMC9869822,Figure_1,oa_package/1e/03/PMC9869822.tar.gz,"['1).', 'Primary syphilis (chancre) of the urethral meatusGenital ulcers prompt a broad differential diagnosis, but certain features are sensitive and/or specific to syphilitic chancres.']",Fig. 1 Primary syphilis (chancre) of the urethral meatus,yes
PMC5888178,Figure_3,oa_package/54/d1/PMC5888178.tar.gz,['CXCL4 induces IL 17 production in autologous antigen presenting cells (APCs) CD4+ T cells co culture.'],"Figure 3 CXCL4 induces IL17 production in autologous antigenpresenting cells (APCs)CD4 Tcells coculture. Monocytes, B cells, myeloid dendritic cells (mDCs), plasmacytoid dendritic cells (pDCs), and CD4 Tcells were isolated from healthy individuals, cocultured in the absence or presence of superantigen from Staphylococcal enterotoxin B (SEB) and CXCL4 for three days and restimulated with PMA and ionomycin. (A) Supernatant from coculture of monocytes and CD4 Tcells stimulated with superantigen SEB and CXCL4 were measured for IL17, IL22, IFN, and IL5. (B) The effect of CXCL4 treatment on 100 pg/mL superantigen SEBactivated CD4 Tcells cocultured with myeloid dendritic cells (mDCs), plasmacytoid dendritic cells (pDCs), or B cells, on IL17, IL22, IFN, and IL5 production was assessed. Cytokines produced were determined using a Luminexbased assay. Means (bars) and values from each donor are shown. Data are pooled from two to five independent experiments, with one to four donor samples in duplicate per experiment. Each dot on the bar graphs represent a single donor paired test was used for statistical analysis. * <0.05, ** <0.01.",yes
PMC11586248,Figure_3,oa_package/9c/84/PMC11586248.tar.gz,"['Uterine inversion observed during the speculum examination, following the endoloop excision of the uterine mass.']","Figure 3 Uterine inversion observed during the speculum examination, following the endoloop excision of the uterine mass. The blue arrow points to the fundus of the endometrium protruding into the vagina.",yes
PMC6098603,Figure_4,oa_package/68/59/PMC6098603.tar.gz,"[' 4).', 'Gross anatomy outcome was assessed by visual examination of joint surfaces.', '05)On the whole, histopathology revealed findings related to damaged cartilage such as fibrillation and chondrocyte loss, and signs of synovium inflammation like cellular infiltration or subintimal changes.']","Fig. 4 Gross anatomy outcome was assessed by visual examination of joint surfaces. Representative images of the macroscopic appearance of the articular cartilage surface in one animal from each group (control, MSC-nave, MSC-primed) at both end-points (two and six months). Assigned scores in these images were 3 (control, both 2 and 6months), 0 (MSC-nave, 2months), 1 (MSC-nave, 6months; MSC-primed, 2months) and 2 (MSC primed, 6months). Mean SEM of the gross anatomy score for each group at two months end-point (phase 2 lesion, light grey bars; control, =4 radio-carpal [RC]-joints; MSC-nave, =7 RC-joints; MSC-primed, =7 RC-joints) and at six months end-point (phase 1 lesion, dark grey bars; control, =4 RC-joints; MSC-nave, =7 RC-joints; MSC-primed, =7 RC-joints). The highest possible score is 4. (*= <0.05)",yes
PMC10705149,Figure_10,oa_package/d9/f7/PMC10705149.tar.gz,[],"Figure 10 Anatomical specimen with the normal C7 cervical vertebra ( ) and schematic representations of the three different grades indicated in green (( ) corresponds to grade 1, ( ) corresponds to grade 2, ( ) corresponds to grade 3 of the classification system for C7), in the first column from left lateral (90) and in the third column from 20 to 45 left-ventral to right-dorsal oblique. In the second and fourth column are radiographs of different horses showing a normal C7 ( ), a C7 grade 1 ( ), a C7 grade 2 ( ) and a C7 grade 3 ( ) in the left latero-lateral projection (second column) and 20 to 45 left-ventral to right-dorsal oblique projection. The asterisk in the radiograph (( ) in fourth column) marks the transverse process of C7. T1 and right side R are marked in the radiograph (( ), second column) and C7 and T1 are marked in the radiograph (( ), second column).",yes
PMC10043603,Figure_2,oa_package/69/a0/PMC10043603.tar.gz,"['Importantly, the patient did not present any axillary adenopathy in the previous mammographic screening round, in 2017 ().', 'g001"" position=""float""/>.']","Figure 2. Right and left craniocaudal ( ) and mediolateral oblique ( ) mammographic views performed in July 2017. Neither enlarged axillary lymph nodes, nor breast suspicious findings are visible.",yes
PMC10474529,Figure_12,oa_package/51/b4/PMC10474529.tar.gz,[],Figure 12 Comparison of alveolar ridge height before and after surgery,yes
PMC6206372,Figure_6,oa_package/7c/2a/PMC6206372.tar.gz,"[' 6)MRI is a noninvasive and nonionizing modality that provides excellent soft tissue resolution.', 'MRI imaging of facet joints.', 'T2 STIR and T1 gado axial views (d, e): articular process bone edemaSingle-photon emission computed tomography (SPECT)The detection of FJ inflammation may be more useful than morphological imaging of the joint itself.']","Fig. 6 Active synovial inflammation and intra articular edema: axial and sagittal T2 STIR views ( , ) and T2 sagittal view ( ). T2 STIR and T1 gado axial views ( , ): articular process bone edema",yes
PMC8602945,Figure_3,oa_package/df/d9/PMC8602945.tar.gz,['.'],"Fig. 3. (A) Enriched pathway analysis demonstrates upregulated and downregulated pathways in TSC-3D compared with TOs. The gene ratio is the number of DEGs identified divided by the total genes in each pathway. Blue and red colors signify the adjusted -values. (B) H&E of TOs ( =4) and TSC-3D ( =5) of different sizes. (C) IHC of CDH1 (mononuclear trophoblast marker) and TFRC (SCT marker). For reference, first-trimester villi are shown stained for each marker. CDH1 is localized to VCT and TFRC to the syncytial brush border. TOs ( =2) are positive for both markers, with the outer cells stained for CDH1 and the inner cells lining the luminal spaces stained for TFRC. In contrast, the majority of the cells in TSC-3D ( =2) are positive for CDH1, with only small foci positive for TFRC. (D) ELISA to measure the production by SCT of the pregnancy hormone, hCG-, by TOs ( =4) and TSC-3D cultures ( =5) (48h conditioned medium collected from cultures with comparable numbers of organoids at similar sizes). TSC-3D secrete significantly less hCG- compared with TOs (data are means.e.m., unpaired two-tailed Student's -test, * =0.029). Scale bars: 100m in B,C.",yes
PMC10634011,Figure_2,oa_package/f6/4e/PMC10634011.tar.gz,"[' 2).', 'Schematic diagram showing annotated data and hyper-parameter tuning steps for the a screening classifier and b region-specific tau classifiers.', 'c For each loop through the stratified tenfold cross validation (CV), data normalization, feature selection using feature recursive elimination with a random forest and the hyper-parameter tuning of the random forest were carried outFor the cortical tau classifier, training objects were sampled from boxes defined over areas of high tau burden, yielding 3954 objects (661 CB, 126 NFT, 254 TA, 2913 TF).', ' 2).']","Fig. 2 Schematic diagram showing annotated data and hyper-parameter tuning steps for the screening classifier and region-specific tau classifiers. For each loop through the stratified tenfold cross validation (CV), data normalization, feature selection using feature recursive elimination with a random forest and the hyper-parameter tuning of the random forest were carried out",yes
PMC11621107,Figure_5,oa_package/85/57/PMC11621107.tar.gz,[],"Figure5 inoculated mice had decreased activated and mucosal CD8+ T cells in the vaginal tract compared to inoculated mice. Female mice were intravaginally inoculated with 10 CFU , , or PBS as a no-exogenous bacteria-negative control every 48 h for 10 days. On day 10 of the experiment, the vaginal tissue (VAG) was collected, processed, and stimulated for 16 h. Cells were stained for Live/Dead staining, CD3, CD8, CD44, CD69, and CD103, and ran on the Cytoflex flow cytometer and analyzed using FloJo software. The percent population and absolute number of CD8+ T cells are shown in panel . The percent population and absolute number of CD44+CD8+ T cells , CD69+CD8+ T cells , and CD103+CD8+ T cells are depicted as well. Data are from n=5 per group, from one experiment representing three independent experiments. Data was analyzed using a one-way ANOVA with Tukeys multiple comparisons (*p<0.05).",yes
PMC10858775,Figure_3,oa_package/de/ce/PMC10858775.tar.gz,"['Magnetic resonance imaging (MRI) of the brain revealed a swelling in the frontal region that was seen involving both the inner and outer table of the frontal bone, causing the widening of the diploic spaces [].', ':(a) Magnetic resonance imaging T2 sagittal and (b) T1 contrast sagittal.']",Figure 3: (a) Magnetic resonance imaging T2 sagittal and (b) T1 contrast sagittal.,yes
PMC4149211,Figure_1,oa_package/d0/c3/PMC4149211.tar.gz,"['Patients with mutations in MLL3, KDM6A, and GPS2 had worse outcomes in terms of overall survival (OS) and disease-free survival (DFS) than those without mutations ( 1).', '\nKaplan-Meier survival curves of 57 patients with and without mutations in chromatin remodeling genes.', '\nKaplan-Meier survival curves combining molecular risk factors shown to be significant in the cohort of 57 patients.']","Figure 1 P-values are calculated with the log-rank test. The red plot indicates survival of patients with mutations in or . Mutations were identified in a pipeline that consisted of the amplification-based Fluidigm Access Array system, massively-parallel sequencing of the exons of select genes with the Illumina MiSeq (2x250), and post-sequencing variant detection followed by annotation with the FreeBayes and ANNOVAR software packages, respectively. Overall survival (OS) measured in the standard-risk (SR) group. OS across all patients. Disease-free survival (DFS) in the SR group. DFS in all patients.",yes
PMC11598744,Figure_5,oa_package/cb/a3/PMC11598744.tar.gz,[],"FIGURE 5 FTS alleviates A pathology by reducing A production and promoting A degradation. (A) Expression of phosphoJNK in the hippocampus of control mice and A treated mice, ** <0.01 vs. control mice. (B) Expression of phosphoJNK in the hippocampus of A mice with or without FTS and Gal3AAV treatment; ** <0.01 vs. A mice; ## <0.01 vs. A mice treated with FTS. (C) Expression of PS1 in the hippocampus of control mice and A treated mice, ** <0.01 vs. control mice. (D) Expression of PS1 in the hippocampus of A mice with or without FTS, anisomycin, or Gal3AAV treatment; ** <0.01 vs. A mice; <0.01 vs. A mice treated with FTS. (E) Amounts of A oligomerization in A mice with or without FTS and anisomycin treatment. * <0.05 and ** <0.01 vs. A mice, <0.01 vs. A mice treated with FTS. (F) Expression of IDE in the hippocampus of control mice and A treated mice, ** <0.01 vs. control mice. (G) Expression of IDE in the hippocampus of A mice with or without FTS and Gal3AAV treatment, ** <0.01 vs. A mice, ## <0.01 vs. A mice treated with FTS. (H) Expression of NEP in the hippocampus of control mice and A mice treated with vehicle; ** <0.01 vs. control mice. (I) Expression of NEP in the hippocampus of A mice with or without FTS and Gal3AAV treatment; ** <0.01 vs. A mice; ## <0.01 vs. A mice treated with FTS. (J) Expression of TTR in the hippocampus of control mice and A mice treated with vehicle; ** <0.01 vs. control mice. (K) Expression of TTR in the hippocampus of A mice with or without FTS and Gal3AAV treatment; ** <0.01 vs. A mice; <0.01 vs. A mice treated with FTS.",yes
PMC7551250,Figure_1,oa_package/e6/e6/PMC7551250.tar.gz,"['The Akt-mediated inhibition of the FoxO family of transcription factors is reduced by dexamethasone, which prevents Akt phosphorylation and leads to activation of FoxO transcription factors and MuRF1 and MAFbx upregulation [36,37,38] ().', '1007/s40279-014-0151-424791917Sarcopenia induced by glucocorticoid-treated skeletal muscle cells in vitro.']","Figure 1 Sarcopenia induced by glucocorticoid-treated skeletal muscle cells in vitro. ( ) Protein kinase B (Akt) causes phosphorylation and nuclear exclusion of Forkhead box protein O (FoxO) family, which suppresses atrogene (muscle atrophy F-box, MAFbx) expression and proteolysis. Dexamethasone prevents Akt-mediated phosphorylation of FoxO (FoxO-P), inducing its transfer to the nucleus and the consequent MAFbx upregulation and protein degradation. ( ) The skeletal muscle structure. ( ) Representative images of human myotube after dexamethasone (Dexa) treatment (50 M for 48 h), showing fluorescence double labeling, using an anti-myosin heavy chain-2 antibody (green), and Hoechst (nuclei in blue). Scale bar = 100 m.",yes
PMC6557619,Figure_7,oa_package/0e/50/PMC6557619.tar.gz,"['Of other cell characteristics, most common were clumped AF granules that could be considered pre-aggregates ().', 'Although not as compact, spherical, or sharply bordered as aggregates (A), pre-aggregates appeared separate from the surrounding uniform distribution.', 'Pre-aggregates of autofluorescent granules in RPE of AMD eyes.', 'RPE L/ML are found in drusen67,68 and in the Henle fiber layer (figure 7 of Ref.', '23Systematic unbiased sampling also uncovered a localness of AMD pathology, exemplified by evolution of a flower-like pattern of a central cell ringed by neighbors ().']","Figure 7 Pre-aggregates of autofluorescent granules in RPE of AMD eyes. (A) Red arrow shows a definite aggregate. (AD) Yellow arrows show proposed pre-aggregates, that is, clumps of AF granules that were not as concentrated or sharply delimited as definite aggregates.",yes
PMC5997445,Figure_1,oa_package/98/9e/PMC5997445.tar.gz,"['Clues for valve reopening were clear-cut evidence of movement of both leaflets in a parallel fashion and a substantial (50%) decrease in transvalvular gradients (, 2).', 'TTE image before thrombolysis shows mean gradient of 21.']",Figure 1 TTE image before thrombolysis shows mean gradient of 21.7 mm Hg across stuck prosthetic mitral valve.,yes
PMC4449487,Figure_4,oa_package/6e/f0/PMC4449487.tar.gz,"['FDG PET-CT images of a teenager with fever of unknown origin.', 'The technique also proved useful in identifying the source of FUO in patients with such conditions as vasculitis (), inflammatory bowel disease, sarcoidosis, painless subacute thyroiditis, cholangiolitis, etc6,[68-69].']","Fig. 4 FDG PET-CT images of a teenager with fever of unknown origin. Linear FDG activity in both legs seen on maximum intensity projection (MIP) images (black arrows, left panel) localized to proximal (red arrow), middle (orange arrow) and distal (yellow arrow) femoral arteries on fussed PET-CT images (right panel). Arteritis was subsequently confirmed as the cause of his fever of unknown origin.",yes
PMC9396854,Figure_1,oa_package/22/58/PMC9396854.tar.gz,"[' 1 describe the MRI findings and classification.', 'gif""/> 50%Abnormal tendon signal starting from the bony origin equal to or more than 50 percent of the thicknessIIIFull thickness tear or Bone edemaWhole thickness abnormal tendon signal change of the CET or the presence of abnormal bony signal at the lateral epicondyleMeasurement of the CET thickness tear in Coronal T-2 weighted fat-suppressed on the image depicting the maximum degree of tear from the inside to the outside CETMRI illustration for the degree of common extensor tendon injuries.', 'The measurement in Coronal T-2 weighted fat-suppressed images shows the maximum degree of tear from the inside to the outside CET (a) type I-tendinosis (b) type IIA-partial thickness tear less than 50% (c) type IIB-partial thickness tear more than 50% (d) type III-full thickness tear (e) type III-bone bruise lesionData analysisMedCalc Statistical Software version 19.']",Fig. 1 MRI illustration for the degree of common extensor tendon injuries. The measurement in Coronal T-2 weighted fat-suppressed images shows the maximum degree of tear from the inside to the outside CET ( ) type I-tendinosis ( ) type IIA-partial thickness tear less than 50% ( ) type IIB-partial thickness tear more than 50% ( ) type III-full thickness tear ( ) type III-bone bruise lesion,yes
PMC9583743,Figure_2,oa_package/8f/79/PMC9583743.tar.gz,"['Intravitreal injections of rAAV2/2-Y444F virus vector encoding the fluorescent reporter construct (mini-mGluR6-mCherry) into XLRS mouse retina did not show any expression of mCherry either in photoreceptor soma or in photoreceptor inner and outer segments ().', '25 Double immunostaining with antibodies against calretinin showed no off-target labeling of ganglion cells or amacrine cells ().', '.', '3A, B) was surprising because the mini-mGluR6-RS1 promoter usually gave expression restricted to RBCs of inner retina (), and this was thought likely from diffusion of RS1 from the inner to the outer retina.']","Figure 2. . Retinal expression of fluorescent transgenes mCherry was examined 5 weeks after intravitreal injection of In4s-In3-200En-mGluR500P-mCherry (mini-mGluR6-mCherry) into XLRS mouse retina. mCherry expression was restricted to cells in the inner retina, most predominately in RBCs. There is no labeling of mCherry in the photoreceptor layer. Vertical sections of XLRS mouse retinas were colabelled for mCherry ( ), protein kinase C ( ), RBC marker; calretinin ( ), amacrine and ganglion cell marker. DAPI was used as a nuclear counterstain ( ).",yes
PMC9569153,Figure_4,oa_package/f6/0b/PMC9569153.tar.gz,[],Figure 4 Left inferior epigastric artery (marked with an arrow) before embolization,yes
PMC6590197,Figure_5,oa_package/92/74/PMC6590197.tar.gz,['Re expression of microRNA 374b target genes inhibits EndMT.'],"Figure 5 Reexpression of microRNA374b target genes inhibits EndMT. (A) The expression of miR374b target genes was restored by lentiviral transformation using plasmids encoding the coding sequences (CDS) of MAP3K3, MAPK7, MEF2D, and KLF4 in endothelial cells that overexpressed miR374b. The (re)expression of these genes was confirmed by RTqPCR. (BD) Endothelial cells that express miR374b decreased their expression of VEcadherin and increased their expression of SM22. These effects were counteracted by the expression of MAPK7 signaling members. (E) Endothelial cells that expressed MAP3K3, MAPK7, MEF2D, or KLF4 have an enhanced angiogenic sprouting capacity and (F) decreased contractile capacity compared to endothelial cell that express miR374b. The levels of angiogenic sprouting and contractile behavior were similar to that of endothelial cells transformed using scrambled control sequences. All = 5, oneway ANOVA, * < 0.05, ** < 0.01, *** < 0.001.",yes
PMC5977133,Figure_6,oa_package/b5/83/PMC5977133.tar.gz,"['Hepatic artery to portal vein fistula can be caused by PTBD and may manifest with hemobilia [17,48] ().', '72-year-old male with a hepatic artery to portal vein fistula.']","Figure 6 72-year-old male with a hepatic artery to portal vein fistula. This patient underwent PTBD placement for biliary diversion after a cholecystectomy led to a cystic duct stump leak. He had intermittent episodes of small volume hemobilia. ( ) Computed tomography angiography (CTA) was performed and showed early opacification of a portal vein branch (curved arrow) for which he was referred for hepatic arteriogram. Celiac ( ) and selective right hepatic ( ) artery angiograms demonstrated hepatic artery (straight black arrows) to portal vein (curved black arrows) fistula, relatively remote from the PTBD. This injury was therefore thought to be related to initial needle passes. ( ) The hepatic artery supplying this fistula was coil-embolized (white arrows). ( ) Repeat hepatic angiogram demonstrated cessation of the arteriovenous fistula.",yes
PMC7769794,Figure_4,oa_package/d9/be/PMC7769794.tar.gz,['A lung window CT image of an inferior portion of the thorax showing pneumopericardium (arrow).'],Figure 3 A lung window CT image of a superior portion of the thorax showing pneumomediastinum (arrows).,yes
PMC10561727,Figure_8,oa_package/34/56/PMC10561727.tar.gz,"['4-fold above background, relative to control PBS-treated animal hearts (A), indicating that a single i.', '8 % of the endogenous cBIN1 transcript levels (B).', 'Low-dose therapy, such as that less than 1 1012 vg/kg, offered significant potential to translate cBIN1 therapy to humans () (28, 39).', 'I.']",Figure 8 I.v. AAV9-cBIN1 (6 10 vg/kg) transduces exogenous cBIN1 in minipig hearts. ( ) Cardiac expression of exogenous cBin1-V5 (Ct of V5/HPRT1 when compared with PBS controls) in minipig hearts 6 months after AAV9-cBIN1 injection. ( ) Exogenous cBin1-V5 as percentage of endogenous porcine cBIN1 (derived from Ct of V5/cBIN1) in minipig hearts 6 months after AAV9-cBIN1 injection ( = 10 tissue samples across left ventricle obtained from 2 minipigs per group). Data are presented as mean SEM. Unpaired 2-tailed Students test or Mann-Whitney test was used. *** indicates < 0.001 for comparison versus PBS controls.,yes
PMC11323873,Figure_1,oa_package/7a/d2/PMC11323873.tar.gz,"['High-resolution NPC structures have recently been revealed using techniques such as advanced cryo-electron microscopy (cryo-EM) and modeling assisted by artificial intelligence [4 10], showing how Nups assemble into diverse subcomplexes that form an intricate modular architecture characterized by eightfold rotational symmetry ().', '.', 'The second group encompasses the peripheral or central Nups, such as those building the central channel, cytoplasmic filaments, and the nuclear basket [11,12] ().', 'A subset of nucleoporins, termed FG-Nups, possess intrinsically disordered regions (IDRs) characterized by phenylalanine-glycine (FG) repeats, which are predominantly found within the peripheral Nups or in the central channel [17] ().']",Figure 1. Structural organization and subcomplexes of vertebrate NPC.,yes
PMC6599257,Figure_6,oa_package/80/79/PMC6599257.tar.gz,"[' 6).', 'Comparative analysis of histology in human and mice pericardial adhesion tissue.', '5 mg/g) for two weeks into the pericardial cavityDiscussionAlthough pericardial adhesions are a common consequence of cardiac surgery, medical and surgical therapies have not yet been established because the molecular mechanism by which adhesions develop is unclear.']",Fig. 6 Comparative analysis of histology in human and mice pericardial adhesion tissue. Hematoxylin and eosin staining was performed to compare human and mouse adhesion tissues from the pericardium. The upper panel shows human heart specimens taken from autopsy patients with left ventricular assist devices. The lower panel shows murine heart samples treated with injection of 500L of talc (2.5mg/g) for two weeks into the pericardial cavity,yes
PMC5501942,Figure_3,oa_package/bb/c3/PMC5501942.tar.gz,"[' 3).', ' 3).', 'Association of neuron-specific enolase (NSE) with poor neurological outcomes (CPC 3 5).', ' AUC area under the curve, CPC Cerebral Performance Category, MAX maximum\nTable 2Comparison of ROC curves for day-specific neuron-specific enolase prediction of poor neurological outcome at 30 daysROCDifference AUC95% Confidence interval\nP\nDay 1 Day 20.']","Fig. 3 Association of neuron-specific enolase (NSE) with poor neurological outcomes (CPC 35). Receiver operating characteristic (ROC) curve for NSE values at Day 1 is schown on Panel ;ROC curve for NSE values at Day 2 is schown on Panel ;ROC curve for NSE values at Day 3 is schown on Panel ;ROC curve for NSE values at Day 4 is schown on Panel ;ROC curve for maximal NSE values is schown on Panel . area under the curve, Cerebral Performance Category, maximum",yes
PMC10580690,Figure_1,oa_package/d5/7b/PMC10580690.tar.gz,[],"FIGURE 1 ECG image demonstrating a sinus rhythm with second degree Mobitz Type I AV block.65 BPM, QRS axis 67, T axis 46, RR interval 1131ms, QRS duration 88ms, QT interval 404ms, corrected QT 381ms.",yes
PMC2895865,Figure_3,oa_package/f9/71/PMC2895865.tar.gz,"[' 3) and an integrated PET-CT scan (Figs.', ' 3).', '', 'Two foci of FDG activity in the retroperitoneum correspond to normal kidneys (K)\n']","Fig.3 A 14-year-old girl with alveolar RMS of the pelvis and pancreatic metastases. Axial contrast-enhanced abdominal CT image demonstrates a small, hypodense mass in the body of the pancreas, which was initially missed on CECTs and detected on PET-CT imaging ( ). Axial CT obtained during PET-CT shows the area of the pancreas metastasis ( ) and subcutaneous ( ) and peritoneal metastases ( ). Co-registered axial PET image, at the same level shown in ( ), demonstrates small, intensely FDG-avid pancreas ( ) and subcutaneous ( ) and peritoneal ( ) metastases. Two foci of FDG activity in the retroperitoneum correspond to normal kidneys ( )",yes
PMC7543793,Figure_7,oa_package/a2/e5/PMC7543793.tar.gz,"['A comparison between our model and U-NET for infection segmentation.', '', ' shows a comparison between our model and U-NET for infection segmentation.']","Fig. 7 A comparison between our model and U-NET for infection segmentation. From left to right columns: input images, ground truth, results of U-NET, results of our method.",yes
PMC4553736,Figure_2,oa_package/67/6c/PMC4553736.tar.gz,[],Figure 2a and b Magnetic resonance imaging T2-WI showing T2 hyper intensity within the temporalis muscle that is hypo on T1-WI,yes
PMC8635056,Figure_6,oa_package/a9/58/PMC8635056.tar.gz,"['The results showed that 998 genes were up-regulated and 409 genes were down-regulated in neurospheres transfected with shTtyh1 (A).', '05 and fold change 2 as a standard, the top 30 most significantly increased or decreased genes were listed and displayed with a heatmap (B).', 'Ccnd1 (cyclin D1) was among the top 30 elevated genes (B, P = 1.', 'Moreover, Ttyh1 knockdown significantly downregulated Id3 and Clu, markers of qNSCs (E, P = 8.', 'Ttyh1 knockdown resulted in significantly up-regulated expression of EGFR (E), a typical signature of the transition from qNSCs to aNSCs (Dulken et al.', 'The decrease of astrocyte markers Atp1a2, Gja1, and Ntsr2 (E, P = 0.', 'Gene Ontology (GO) analysis suggests that the differentially expressed genes are mainly involved in neuron projection, ion channel complexes, and the structure of the plasma membrane, and are involved in the development of the nervous system, the mitotic cell cycle, and the regulation of cell communication (C), consistent with previous studies (Kumada et al.', 'KEGG pathway analysis showed that after Ttyh1 knockdown, many signal pathways such as calcium ion signal pathway, p53 signal pathway, PI3K-Akt signal pathway, and cell cycle were activated, among which calcium signal pathway was the most significant one (D).', 'GSEA analysis confirmed these findings (F).']","FIGURE 5 Ttyh1 prevents accelerated exhaustion of the elderly NSCs pool. Immunofluorescence staining of brain sections of elder control and Ttyh1 KO mice at 12-months old (12M). Sox2 GFAP NSCs, EGFR TAPs, DCX neuroblasts, Ki67 proliferating cells and CD24 ependymal cells were compared in neurogenic niche between control and Ttyh1 KO mice. Statistics of immunofluorescence staining results in A. Scale bar = 50m in A. = 4 for all experiments. Data are expressed as mean SEM. Statistical significance was calculated using an unpaired, two-tailed Students -test. () < 0.05.",yes
PMC9615153,Figure_2,oa_package/10/47/PMC9615153.tar.gz,"[' 2A).', 'Immunohistochemical staining of CD68.', '(B, 100)Immunohistochemical staining of ABCA1 and ACAC1.']","Fig. 2 Immunohistochemical staining of CD68. The expression of CD68 was not found in ischemic mitral valve ( , 40) but was found in rheumatic mitral valve n. ( , 100)",yes
PMC7548525,Figure_2,oa_package/3f/00/PMC7548525.tar.gz,"[' 2, 3, 4, 5, and 6.', '918', ' 2A 50-year-old male, COVID-19 PCR positive, presented with bilateral lower lobe ground glass opacities (arrows) with no intraabdominal pathology reported (not shown)']","Fig.2 A 50-year-old male, COVID-19 PCR positive, presented with bilateral lower lobe ground glass opacities (arrows) with no intraabdominal pathology reported (not shown)",yes
PMC9768437,Figure_1,oa_package/18/e9/PMC9768437.tar.gz,[],"Figure1 Construction of vaccine plasmids that can express fusion proteins. Schematic of the candidate DNA vaccine construction strategy. Enzymatic ligation was used to introduce the matching pre-defined antigen fragment into the multiple cloning site (MCS) of the plasmid. HEK293T cells were transfected with the indicated plasmids, and the expression of fusion proteins was detected by Western blot. RAW264.7 cells were transfected with recombinant plasmids, and secretion of TNF- and IL-6 was detected by ELISA. Data are shown as meanSD, n = 3. Statistical analyses performed by two-way ANOVA (ns, No significance; * < 0.05; ** < 0.01).",yes
PMC10756656,Figure_5,oa_package/27/88/PMC10756656.tar.gz,"['Axial and coronal sections of post-contrast T1-weighted maximum intensity projection images showing the lesion abutting the M1 segment of the left middle cerebral artery (arrows in A, B).']","Figure 5 Axial and coronal sections of post-contrast T1-weighted maximum intensity projection images showing the lesion abutting the M1 segment of the left middle cerebral artery (arrows in A, B).",yes
PMC11548667,Figure_2,oa_package/36/5d/PMC11548667.tar.gz,"['This process facilitated the optimal utilization of visual data for the training of CNNs, illustrated in the subsequent .', 'Subsequent to cropping, the images detailed in were re-evaluated by a pathologist (B.', '(a) PDAC/CP image; (b) the illustration demonstrates the selection of normalized tissue patches based on the CNN classification; (c) selected images used after the data cleanup.']",Figure 2 ( ) PDAC/CP image; ( ) the illustration demonstrates the selection of normalized tissue patches based on the CNN classification; ( ) selected images used after the data cleanup.,yes
PMC9393646,Figure_2,oa_package/55/4d/PMC9393646.tar.gz,[],"Figure2 Assessing the composition of the primary melanoma intratumoral immune infiltrate finds CD39+CD103+PD-1- (P2) CD8+ T cells and B cells associated with improved outcome. Two sections of primary melanoma FFPE samples were separately stained for CD8+ T cells and immune cells using Opal mIHC. Cells from separate sections were aligned to create a single spatial plot for each tumor. (n=64) 20X resolution images of sections stained for T cell panel (left) and immune cell panel (right). All immune cell populations were quantified per mm of tumor. Statistical differences were calculated using a Mann-Whitney unpaired non-parametric test. Correlation plot of all immune cell populations, including % of each CD8+ T cell population, % Class I MHC positivity, and cells/mm of T cells, CD8+ T cells, B cells, NK cells and Langerhans cells (LC). Tumor composition, intratumoral immune population composition, and % of Class I MHC+ melanoma were calculated. Each of the 8 CD8+ T cell populations were quantified as a % of the total intratumoral CD8+ T cell compartment. Composition of the intratumoral CD8+ T cell compartment overall was compared based on patient outcome. Composition of the intratumoral CD8+ T cell compartment for individual patients. *p<0.05; **p<0.01, ***p<0.001.",yes
PMC9478255,Figure_5,oa_package/94/d1/PMC9478255.tar.gz,['.'],"Figure 5. Pancreas solid serous cystadenoma in a 70-year-old female patient with abdominal pain. (A) Microscopic examination showed a tumor condensed area with thick fibrous bands and multiple microcysts lined by flat to cuboidal epithelial cells (hematoxylin & eosin, 200). (B) Unenhanced CT image demonstrating a low-density mass in pancreas head. (C~E) Arterial, portal venous, and delayed phases of contrast-enhanced computed tomography images showing wash-in and wash-out enhancement patterns. CT = computed tomography.",yes
PMC8402752,Figure_1,oa_package/58/cb/PMC8402752.tar.gz,"['During necropsy, external examination of the carcass revealed no changes except peripheral lymph node enlargement (a).', 'A few pinpoint hemorrhages involved the epiglottis while tonsils were slightly hyperemic (b).', 'Pulmonary edema was so severe that white frothy fluid was noticed up to the larynx region, while the caudal mediastinal lymph nodes were dark red in color and moderately swollen (c).', 'Multiple petechial and ecchymotic hemorrhages were present on the left auricle and the subendocardial layer of the right ventricle (d,e).', 'In the abdominal cavity, abundant serosanguineous fluid with scattered fibrin fibers was observed (f).', '02529486878(a).', 'A few petechiae are observed on the epiglottis (black arrows), the tonsils are mildly enlarged and on the cut surface are shown formations similar to those described in a for inguinal lymph nodes (white arrows).']","Figure 1 ( ). Enlarged superficial cervical (prescapular) (P), submandibular (S), and inguinal (I) lymph nodes. The submandibular lymph nodes appear also diffusely hyperemic and the inguinal on the cut surface are characterized by hemorrhage at the periphery of lymphoid follicles, probably in the marginal zone (arrows). ( ). A few petechiae are observed on the epiglottis (black arrows), the tonsils are mildly enlarged and on the cut surface are shown formations similar to those described in a for inguinal lymph nodes (white arrows). ( ). The right lung architecture is locally distorted because of fibrosis and the presence of symphysis between visceral and parietal pleura. Lungs are diffusely hyperemic and frothy material (pulmonary edema) occupies the tracheal lumen (black arrow). The caudal mediastinal lymph nodes are hemorrhagic (white arrows). ( ). Multiple subepicardial petechial and ecchymotic hemorrhages are noted in the right auricle of the heart. ( ). Hemorrhages are located mostly in the subendocardium of the left ventricle. ( ). The peritoneal cavity bears serosanguineous content with a few filaments of fibrin. The spleen is discernible on the right side of the abdomen due to enlargement and presents dark red to black color.",yes
PMC11497288,Figure_1,oa_package/66/b9/PMC11497288.tar.gz,[],"FIGURE 1 Highresolution array tomography (AT) reveals synaptic pathology in ageing and AD. (A) Synapses were quantified from AT image stacks and counted as a synaptic pair if the centroid of the presynaptic object (synaptophysin, magenta) was within 0.5mm of the nearest postsynaptic object (PSD95, cyan). Arrows show examples of synaptic pairs.(B) The density of paired synapses decreases between mid life (ML), healthy ageing (HA), and Alzheimer's disease (AD) and a linear mixed effects model followed by ANOVA shows there was an effect of cohort F[2,36.79]=7.02, =0.002. There were no differences in synapse density between males and females, or carriers and noncarriers. Pairwise posthoc comparisons showed synapses were significantly decreased in the AD cohort in comparison to ML and this was evident in both brain regions (BA20/21, t ratio=3.02; =0.005, d=68; BA17, t ratio=3.04; =0.009, d=59). Synapses were also decreased in HA in comparison to ML in BA17 (t ratio=2.76; =0.02, d=57). We examined the synaptic localization of A (grey, C) and tau (yellow, D). In presynaptic terminals, there was a trend towards an increase in A accumulation between midlife, healthy agers, and AD (E, F[2,34.95]=3.30, =0.04) and a significant increase in presynaptic A in regions containing plaques (F[2,164.15]=15.39, <0.0001). Pairwise posthoc comparisons showed a significant increase in A accumulation in AD BA17 (t ratio=2.60; =0.03, d=69). In postsynaptic terminals, there was a significant increase in accumulation of A between ML, HA, and AD (F, F[2,37.25]=4.39, =0.01) and a significant increase in postsynaptic A in regions containing plaques (F[2,157.72]=12.23, <0.0001). Pairwise posthoc comparisons showed a significant increase in A accumulation in AD BA17 (t ratio=2.88; =0.01, d=63). Presynaptic tau accumulation was significantly increased in AD (G, F[2,32.41]=25.50, <0.0001) and significantly higher in BA20/21 than in BA17 (F[1,135.23]=25.69, <0.001). There was also a significant interaction between cohort and brain region (F[2,127.08]=5.75, =0.004). Pairwise posthoc comparisons showed a significant increase in presynaptic tau accumulation in AD in comparison to ML and HA cohorts and this was evident in both regions (BA20/21, ML v AD (t ratio=5.67; <0.0001, d=64); HA v AD (t ratio=6.75; <0.0001, d=52); BA17, ML v AD (t ratio=3.95; =0.0006, d=56); HA v AD (t ratio=3.95; =0.0007, d=50). Postsynaptic tau accumulation increases from ML to HA to AD groups (H, F[2,31.81]=28.65, <0.0001) and is higher in BA20/21 than BA17 (F[1,135.56]=31.78, <0.001). There is also an interaction between brain region and cohort (F[2,126.91]=7.83, =0.0006). Pairwise posthoc comparisons showed a significant increase in postsynaptic tau accumulation in AD in comparison to ML and HA cohorts and this was evident in both regions (BA20/21, ML v AD (t ratio=5.92; <0.0001, d=63); HA v AD (t ratio=7.40; <0.0001, d=52); BA17, ML v AD (t ratio=4.13; =0.0004, d=55); HA v AD (t ratio=4.15; =0.0004, d=50). For boxplots, each point represents case medians. Type III ANOVA with Satterthwaite correction were performed on the linear mixed effects models. Scale bar 1m for IMARIS reconstructions. * Represent <0.05 posthoc comparisons",yes
PMC4365885,Figure_4,oa_package/60/c1/PMC4365885.tar.gz,[],"Fig 4 Transplanted BM HSCM integrate into AD model mouse brain and reduce A burden BM HSCM obtained from eGFP-mice were injected unilaterally into hippocampus, using contralateral vehicle-injection as a control (schematic figure). The area depicted in grey is enlarged below (AE). Transplanted HSCM migrated along the granular layer of dentate gyrus and the molecular layer of lacunosum moleculare and invaded the parenchyma with variable extent. AD (APdE9 , black columns) brain conditions enhanced the survival of HSCM when compared to their wild-type (APdE9 , open columns) littermates (A, < 0.05, = 5 for AD and = 4 for WT mice). BM HSCM reduced A burden at the site of transplanted cells (B, < 0.001, = 5). Cell transplantation did not increase CD68, Iba-1 or GFAP immunoreactivity (CE). Scale bar = 100 m.",yes
PMC6823454,Figure_3,oa_package/21/28/PMC6823454.tar.gz,"[' 3(A C).', ' 3B), but was not blocked by 1 M of the beta sheeted amyloid-marker Pittsburgh compound B (PIB; ', ' 3C).', 'Representative autoradiograms of [18F]Flortaucipir binding sites.', 'Within each brain area analysed, there was a positive correlation between the age-dependent increase in the binding levels of [18F]Flortaucipir and the progressive increases in the density of Gallyas-positive lesions (Pearson r for all brain regions: 0.']","Figure 3 Representative autoradiograms of [ F]Flortaucipir binding sites. ( ) Sagittal brain sections of transgenic (top panel) and wild-type mice (WT, lower panel), taken at the level of the entorhinal cortex [lateral 2.880.12mm of the Paxinos and Franklin mouse atlas ]. Images were analysed on a black & white display mode, and presented as a pseudocolor interpretation of black & white pixel intensity, calibrated in kBq/mL of [ F]Flortaucipir solution. Age-dependent increases in binding levels were observed exclusively in / mice. [ F]Flortaucipir binding in sections from the middle frontal gyrus of an AD-confirmed patient, 18-month-old / mice and 20-month-old Tg2576 animals, showing the magnitude of tau pathology in patient vs. transgenic mouse tissue. Non-specific binding (NSB) was assessed in the presence of 50M cold flortaucipir. Binding was not blocked by co-incubating sections with [ F]Flortaucipir and 1M of the amyloid-targeting agent Pittsburgh Compound B (PIB).",yes
PMC4398828,Figure_2,oa_package/5b/75/PMC4398828.tar.gz,"['Most common MRI finding was diffuse cord expansion at the site of lesion (In all 9 MRI finding) followed by characteristic Target sign (Hypointense on T1, Hyperintense ring enhancing lesion with central hypointensity) found in seven cases ().', 'MRI spine of Case 10 showing well defined regular rim enhancing lesion in anterior aspect of conus at D12-L1 level with mild leptomeningeal enhancement suggestive tuberculoma (A) Sagittal T1 post-contrast, (B) coronal post-contrast and (C) T2 axial images.']","Fig. 2 MRI spine of Case 10 showing well defined regular rim enhancing lesion in anterior aspect of conus at D12-L1 level with mild leptomeningeal enhancement suggestive tuberculoma ( ) Sagittal T1 post-contrast, ( ) coronal post-contrast and ( ) T2 axial images.",yes
PMC8369823,Figure_9,oa_package/fe/e6/PMC8369823.tar.gz,"['Foci of T1- and T2-weighted hypo intensities within the lesion represent mineralised matrix, which can be correlated with radiographs or CT [24] ().', 'Enchondroma: AP radiograph (A) showing ill-defined lesion involving the greater trochanter with internal rings and arcs calcification (arrow) and corresponding coronal STIR (B) showing a lesion with variable signal suggesting fluid, soft tissue, and calcified components (arrow) but no overlying cortex destruction or soft tissue componentChondroblastomaChondroblastoma is a rare, benign chondral lesion affecting the paediatric population, involving centres of endochondral ossification, most commonly seen at epiphyses and apophyses of long bones.']","Figure 9 Enchondroma: AP radiograph ( ) showing ill-defined lesion involving the greater trochanter with internal rings and arcs calcification (arrow) and corresponding coronal STIR ( ) showing a lesion with variable signal suggesting fluid, soft tissue, and calcified components (arrow) but no overlying cortex destruction or soft tissue component",yes
PMC7044032,Figure_2,oa_package/e8/9c/PMC7044032.tar.gz,"['Compared with controls, 3TG HF offspring showed a statistically significant decrease in the levels of A 1 40 and A 1 42 in both fractions ( A).', 'As shown in figure 2E and F, we observed that compared with controls, 12 month-old 3TG HF offspring had a significant lower amyloid plaque burden.', 'No differences were observed between the two groups of mice when the steady state levels of APP, ADAM-10 and the four components of the -secretase complex were assayed ( B, C).', 'By contrast, beta secretase 1 (BACE-1) protein levels were significantly decreased in the brain of 3TG HF compared to control offspring ( B, C).', 'BACE-1 cleavage product sAPP was also reduced in the same group, confirming an effect of the diet on BACE-1 pathway ( B, C).', 'BACE-1 mRNA levels were significantly reduced in brain cortex of 3TG HF offspring compared to controls, suggesting a translational regulation of BACE-1 gene expression by gestational HF diet ( D).', 'Finally, no changes in the steady state levels of CD-10 and IDE, two major A degrading enzymes, or in apoE levels, an A chaperone, were noted between the two groups of mice ( B, C).', ':Effect of gestational high fat diet on brain amyloidosis.']",Figure 2: Effect of gestational high fat diet on brain amyloidosis. A40 and A42 levels in both RIPA soluble and formic acid soluble fractions in brain cortex of 12 months old 3TG HF and 3TG CTR offspring (A). Protein levels of enzymes involved in production and clearance of A measured by western blot analysis in brain cortex of 12 months old 3TG HF and 3TG CTR offspring (B). Densitometry of previous panel (C). BACE-1 mRNA levels measured by rt-PCR in brain cortex of 12 months old 3TG HF and 3TG CTR offspring (D). A plaques burden measured as A (4G8) immunoreactivity in brain sections of 12 months old 3TG HF and 3TG CTR offspring (E). Quantification of immunoreactivity shown in previous panel (F). Results are mean sem. * = p < 0.05. Western blot molecular weight: see .,yes
PMC4277831,Figure_1,oa_package/e0/4c/PMC4277831.tar.gz,"['As shown in 1A, after 24 h we observed the aggregation of full length tau in the vesicles of C17.', '2 cells and SHSY5Y cells (Red arrow in 1B) but not in N2a cells, suggesting cellular specificity for the aggregation of tau.', 'Tau aggregates were seen in N2a cells 72 h after transfection ( 1D), but not in YFP expressing cells, indicating that the aggregates seen in tau-YFP transfected N2a cells were not YFP-dependent, but specific for tau.', 'As shown in 1E, ThS staining completely overlapped with the large tau aggregates highlighted with YFP, but not with diffusible tau in cytoplasm.', '\nExpression and aggregation of Tau441-YFP in three different cell types.', 'A 1-42 increases the level of insoluble tau while A 1-40 has no effectIn vivo, A 1-42 is more prone to forming amyloid plaque than A 1-40.']","Figure 1 SHSY5Y, C17.2 and N2a cells were transfected with Tau441-YFP and live cell images were taken at 24h with a confocal microscope. Darkfield images showed better fluorescence signal and phase contrast provided images for the whole cell. Aggregates formed in SHSY5Y and C17.2 cells (red arrow). Quantification of transfection efficiency was done with Image J. Time course of Tau441-YFP expression and aggregation in N2a cells. Aggregates appeared at 72h after transfection while no aggregates formed in YFP transfected cells, which suggested that those aggregates were tau specific. 72h after transfection, cells were fixed with 4% PFA and stained with thioflavin-S (ThS). Only a small population of cells showed ThS-positive aggregates (red arrow). Cells (green arrow) with diffusely distributed tau were ThS negative even though a high level of Tau441-YFP was expressed in those cells. This further confirmed that the ThS signal was not a false signal due to the leakage of YFP. Magnification: 63x. Scale bar: 10m.",yes
PMC9791507,Figure_10,oa_package/ab/e0/PMC9791507.tar.gz,[],Figure 10 The figure depicts a general improvement in tissue conditions. A: Inter-occlusal view; B: Right side view; C: Left side view.,yes
PMC3321462,Figure_3,oa_package/63/8b/PMC3321462.tar.gz,"['Relatively low intensity on T2-weighted images representative of fibrosis may be an important tool in the accurate diagnosis of this condition [33, 34] ().', '002""/>Pelvic inflammatory disease.']","Figure 3 Pelvic inflammatory disease. Left recurrent tuboovarian abscess. Axial T2-weighted image (a) shows a thick wall, complex, heterogeneously hyperintense, fluid-containing adnexal mass, with internal debris and gas bubbles, that represents the most specific sign of an abscess. On axial T1-weighted with fat suppression image (b) there is no signs of hemorrhagic contents within the mass. The sagittal T2-weighted image (c) demonstrates the involvement of bowel loop characterized by the thickening of the anterior rectal wall.",yes
PMC3088377,Figure_3,oa_package/2a/33/PMC3088377.tar.gz,['.'],Figure 3. Hypertensive intraparenchymal hematoma. Nonenhanced computed tomography shows a large right basal ganglionic hematoma (*) containing a fluid/fluid level (arrow).,yes
PMC2212559,Figure_4,oa_package/40/c7/PMC2212559.tar.gz,['Immunostaining of pPaxillin is increased in rheumatoid arthritis synovial tissue (RA ST) compared to osteoarthritis (OA) and normal donor (ND) ST.'],"Figure 4 Immunostaining of pPaxillin is increased in rheumatoid arthritis synovial tissue (RA ST) compared to osteoarthritis (OA) and normal donor (ND) ST. (a) demonstrates RA ST stained with anti-pPaxillin (200), (b) shows positive staining in OA ST (200). (c) Low pPaxillin reactivity in normal ST lining and subsynovial Ms. (d) Is the quantification data obtained from a, b and c. Bars represent mean and SEM. Inflam, inflammatory score; Vasc, vascularity score; Lining, ST lining cell layer; Mac, subsynovial Ms.",yes
PMC5937311,Figure_6,oa_package/8c/fa/PMC5937311.tar.gz,"['First, 15 (47%) of the 32 knees had articular cartilage changes [].', 'A 55-year-old AAIHP.']","Figure 6 A 55-year-old AAIHP. Sagittal PD-weighted MRI image with fat suppression, showing Grade III changes of the articular cartilage (black solid arrow) and BME (white solid arrow). PD: Proton density; AAIHP: Asymptomatic amateur ice hockey players; MRI: Magnetic resonance imaging; BME: Bone marrow edema.",yes
PMC2857854,Figure_3,oa_package/d7/7a/PMC2857854.tar.gz,['Labial edema.'],Figure 3 . Unenhanced axial computed tomography image depicts extensive bilateral subcutaneous edema (large arrow) with extension into the labia (small arrow).,yes
PMC5069216,Figure_4,oa_package/d3/09/PMC5069216.tar.gz,"[' 4).', 'Operative specimen findings.', 'Operative specimen revealed a splenic tumor measuring 28 22 mm\nHistopathological findings.']",Fig. 4 Operative specimen findings. Operative specimen revealed a splenic tumor measuring 2822mm,yes
PMC7367563,Figure_5,oa_package/72/e4/PMC7367563.tar.gz,"['Doppler demonstrates high flow with arterialization of veins [].', '[13]Axial pulse Doppler showing arterialization of the right middle finger digital vein due to shunting in the CM-AVM syndromeT2 STIR images of the right hand showing flow voids in AVM of the right ring fingerAVMs are known to expand with time, becoming complicated, and infiltrative with its growth.']",Figure 5 Axial pulse Doppler showing arterialization of the right middle finger digital vein due to shunting in the CM-AVM syndrome,yes
PMC4950757,Figure_2,oa_package/ae/60/PMC4950757.tar.gz,"[' 2).', 'Liposomes (1 mg or 100 g) reduced CXCL8 concentration from human nasopharyngeal epithelial cells induced by different pneumococcal strains.', 'Error bars indicate standard deviationLytic antibiotic is required to induce CXCL8 response to serotype 3 strain P21, which was reduced by liposomesUnlike the other wild type clinical isolates in this study, strain P21, serotype 3, has been passaged in an animal model and has an extremely thick capsule.', ' 2).']",Fig. 2 Liposomes (1mg or 100g) reduced CXCL8 concentration from human nasopharyngeal epithelial cells induced by different pneumococcal strains. Values shown are means of three independent experiments and are the values with 1mg or 100g of liposomes expressed as percentages of the CXCL8 concentrations obtained in the absence of liposomes in the presence of wild type clinical isolates. Liposomes reduced the CXCL8 response to all pneumococcal strains tested. Numbers in brackets indicate serotypes. Error bars indicate standard deviation,yes
PMC2830966,Figure_1,oa_package/d9/4f/PMC2830966.tar.gz,"['(a) Serum IGF-I concentrations, and (b) expression of placental mRNA of IGF-I and IGF-IR in gestational diabetic mothers and their babies.']",Figure 1 . Serum IGF-I concentrations and mRNA expression by RT-PCR were assessed as described in the section of the methods. Values are means SD. NS = insignificant differences. n = 60 control mothers and babies; n = 60 gestational diabetic mothers and macrosomic babies.,yes
PMC6677310,Figure_2,oa_package/00/b8/PMC6677310.tar.gz,"['ResultsAverage ultrasonic vocalization frequency dataSpectrograms of representative frequency-modulated USVs for each group are shown in A 2H.', 'g002USV spectrogram.']",10.1371/journal.pone.0220734.g002,yes
PMC4169373,Figure_7,oa_package/24/04/PMC4169373.tar.gz,"['Further, there were ectatic upper dermal blood vessels, focal swelling of endothelium, fibrinoid necrosis of vessel walls, extravasated red cells and presence of fibrin thrombi ().', 'g007Cutaneous histopathology of maculopapular skin rash, (A) Black arrow- Exocytosis of extravasated lymphocytes.']",10.1371/journal.pntd.0003179.g007,yes
PMC5087830,Figure_1,oa_package/78/68/PMC5087830.tar.gz,['.'],"Fig. 1. (A) Quantitative SYBR green real-time PCR was performed for and in lymph node (LN) cells from naive LEW.1AV1 ( ) rats (black bars, =6), or those immunized with CFA (gray bars, =8) or MOG 91-108 in CFA (white bars, =8), on day 12 p.i. Increased (ANOVA, * <0.0001) and (ANOVA, * <0.05) expression was found in MOG 91-108 in CFA- immunized rats compared to naive and CFA-alone-immunized rats. (B) Quantitative expression of in cells eluted from the CNS of CFA (white bars, =6)-, MOG 73-90 in CFA (gray bars, =6)- and MOG 91-108 in CFA ( =6)-immunized LEW.1AV1 ( ) rats. was upregulated in MOG 91-108 in CFA-immunized rats compared to the other groups (ANOVA, * <0.001) on day 12 p.i. (C) Quantitative expression of of lymphocytes eluted from the CNS of CFA (white bars, =6)-, MOG 73-90 in CFA (gray bars, =6)- and MOG 91-108 in CFA ( =6)-immunized LEW.1AV1 ( ) rats on day 12 p.i. was upregulated in MOG 91-108 in CFA-immunized rats compared to the other groups (ANOVA, * <0.001). Results are expressed as 2 values. Numbers are means.e.m.",yes
PMC11536229,Figure_1,oa_package/e8/41/PMC11536229.tar.gz,"['3 cm 7 4 1A 2A 1Tumors showing low-grade features of SDH-RCC SDH-RCCA, The gross appearance of the tumor; B, the tumor cells grew in sheet-like structures and had eosinophilic cytoplasm and intracytoplasmic vacuoles, showing low-grade nuclear features; C, the tumor cells grew in glandular structures; D, the tumor boundary was clear, and entrapped renal tubules were observed; E, the tumor tissue showed negative staining results for SDHB, with vascular endothelium as the positive control; F, the proliferation index of Ki-67 of tumor cells was 5%.', '6% ISUP /WHO 2016 1 2 1B 1D 4 4/11 36.', '4 11 SDH-RCC SDHB 1E 2E 7 6 SDHA 1 SDHA 3 4 SDHA 2 PAX8 FH EMA CK7 CAIX CD117 Ki-67 1% 30% Ki-67 5% 1F Ki-67 20% 30% 2F 3The expression of SDHA protein in SDH-RCC tumor tissueSDH-RCC SDHA HE staining of case 2 (A) and case 3 (C); immunohistochemistry staining showed SDHA negative results in case 2 (B) and SDHA positive results in case 3 (D).']",1 Tumors showing low-grade features of SDH-RCC SDH-RCC,yes
PMC11585472,Figure_4,oa_package/b1/4b/PMC11585472.tar.gz,"['The mass was completely excised, and the postoperative pathological examination revealed features consistent with Castleman disease (hyaline-vascular type) ().', 'Histologic section of the retroperitoneal mass.', 'DiscussionCastleman disease (CD) presented with various clinical and radiological manifestations, poses a significant diagnostic challenge.']",Fig. 4 Histologic section of the retroperitoneal mass. (A) Germinal centers traversed by sclerotic penetrating vessels and hyalinization-lollipop follicles (arrow). (B) Mantle zones are thickened with lymphocytes arranged in layers - onion skin appearance. (C) The inked abdominal mass margin is obvious (*) with muscle fibers and vessels beneath it. Follicle with features of Castleman disease (arrow).,yes
PMC6531305,Figure_6,oa_package/50/c2/PMC6531305.tar.gz,[' NUPR1 promotes angiogenesis through a PDGFA/MEK/ERK signaling cascade.'],"Figure 6 The expression levels of total and phosphorylated forms of PDGFR, MEK1/2, and ERK1/2 proteins in HUVECs cultured with CM from NUPR1-overexpressing, -knockdown, or control Mahlavu and Huh7 cells for 48 h were analyzed by western blotting. GAPDH was used as a loading control. HUVECs were pretreated with DMSO (vehicle control) or the ERK inhibitor U0126 (10 M) for 1 h, then seeded on matrigel together with CM from NUPR1-overexpressing and control Mahlavu cells for 16 h. Chick embryo were treated with DMSO or U0126 for 1 h, after which a mixture of matrigel and CM from NUPR1-overexpressing or control Mahlavu cells was implanted on the chick embryo. After a 4-day incubation, the number of vessel branching points were quantified by image analysis. Serially deleted pA3TK-PDGFA promoter-report constructs and pcDNA3-NUPR1 expression plasmid were co-transfected in 293TN cells, after 24 h, cells were lysed and promoter activity was determined (left panel). Wild type and mutant forms of PDGFA promoter constructs were co-transfected with pcDNA3-NUPR1 plasmid in 293TN cells, after 24 h, cells were lysed and promoter activity was determined (right panel). NUPR1-overexpression Mahlavu cell lysates were immunoprecipitated with nonspecific rabbit IgG or antibodies against NUPR1. GAPDH promoter region was used as negative control, respectively. Bar plot represents means SD (*p < 0.05, **p < 0.01, ***p < 0.001; one-way ANOVA).",yes
PMC4921111,Figure_1,oa_package/1e/44/PMC4921111.tar.gz,"[' 1a).', 'Structures of apoE3 and apoE4.', 'Copyright 2012 American Chemical SocietyApoE3 and apoE4 bind to the LDL receptor with similar affinity (~20-fold greater than that of apoB100, the other LDL receptor ligand).', ' 1a).', ' 1c).', ' 1c).', ' 1b) have been identified with a cellular fluorescence resonance energy transfer (FRET) assay in which the amino-terminal domain of apoE4 is labeled with green fluorescent protein and the carboxyl-terminal domain is labeled with Escherichia coli dihydrofolate reductase.']","Fig. 1 Structures of apoE3 and apoE4. ApoE4 displays domain interaction caused by an ionic interaction between Arg-61 and Glu-255. This structural feature of apoE4 (apoE4 > apoE3 > apoE2) alters its function in cardiovascular and neurological disorders. Small-molecule apoE4 structure correctors block domain interaction and convert apoE4 to an apoE3-like molecule structurally and functionally. Injury of neurons induces apoE expression. When neurons are stressed or injured, they synthesize apoE to function in lipid redistribution for neuronal repair and remodeling. In this model, apoE4 is recognized as structurally abnormal and undergoes proteolytic cleavage, generating several neurotoxic fragments (1229kDa) that escape the secretory pathway, enter the cytosol, and cause mitochondrial dysfunction and tau phosphorylation (Tau-PO ), ultimately causing cell death. ApoE4SC, apoE4 structure corrector. Modified from ref. . Copyright 2012 American Chemical Society",yes
PMC5352173,Figure_3,oa_package/77/e3/PMC5352173.tar.gz,['Self-KIR is expressed on expanding NKG2C+ NK cells during CMV reactivation post haplo-HSCTA.'],"Figure 3 Self-KIR is expressed on expanding NKG2C NK cells during CMV reactivation post haplo-HSCT - KIR and KIR NK cells were distinguished by flow cytometry using antibodies specifically against KIR2DL1, KIR3DL1 and KIR2DL3. The percentage of the CD56 NKG2C NK cells were determined in the KIR () and KIR () populations respectively in patients with ( , = 19) or without CMV reactivation ( , = 10). Values represent mean SEM. * < 0 .05 and ns (not significant) > 0.05 for comparisons between the KIR and KIR groups. - Self-KIR and non-self-KIR were determined based on the patient KIR ligand status. The percentage of the CD56 NKG2C NK cells were assessed by flow cytometry in the self-KIR () and non-self-KIR() patients with ( , = 10 for self-KIR and = 9 for non-self-KIR) or without CMV reactivation ( , = 5 for self-KIR and non-self-KIR each), respectively. Values represent mean SEM. * < 0 .05 and ns (not significant) > 0.05 for comparisons between the self-KIR and non-self-KIR groups.",yes
PMC11303219,Figure_2,oa_package/ef/5f/PMC11303219.tar.gz,[],"Figure2 Case 1, male, 56 years old, MIP/SOL+ group lung AC, irregular solid nodule in the middle lobe of the right lung, with a maximum diameter of about 18mm, with lobulated and spiculated edges and vacuole signs within the lesion; pathology (HE 10) shows invasive lung AC, with micropapillary accounting for approximately 50% and acinar type accounting for approximately 50%. Case 2: Male, 63 years old, lung AC in the MIP/SOL- group; mixed ground-glass nodule in the upper lobe of the left lung, with a maximum diameter of about 15mm, lobulated edges, and adjacent pleural depression; pathology (HE 10) showed invasive lung AC, with approximately 40% lepidic structures and 60% acinar type.",yes
PMC6208250,Figure_1,oa_package/d8/c6/PMC6208250.tar.gz,"['Magnetic resonance imaging (MRI) of the left knee showed a long segmental, high signal intensity lesion on T2-weighted image along the course of the distal common peroneal and proximal deep peroneal nerve [].', 'It did not show any enhancement after gadolinium [].', 'Magnetic resonance imaging of intraneural ganglion cyst involving the common peroneal nerve.', 'Although the transverse limb sign was observed, the signet ring sign was not observed [].']","Figure 1 Magnetic resonance imaging of intraneural ganglion cyst involving the common peroneal nerve. (a) T2-weighted coronal image with fat suppression showing a tubular cyst (arrows) circumferentially surrounding the tibial and peroneal division within the distal sciatic nerve. (b) Axial T2-weighted FSE image at the level of the neck of the fibular showing intraneural cyst (arrows) within the articular branch (arrowhead) of the peroneal nerve with cyst extending into the proximal nerve branch to the tibialis anterior muscle, corresponding to the transvere limb sign F: Fibular, T: Tibia, TA: Tibialis anterior muscle. (c) Axial T2-weighted FSE image above the level of the fibular showing an intraneural ganglion cyst (arrow) in which the tibial and peroneal division are separately contained",yes
PMC6243300,Figure_2,oa_package/8c/3f/PMC6243300.tar.gz,"['In addition, autopsy revealed a comminuted fracture, with no significant displacement of the right zygomatic arch that was not clearly visible on CT examination (s 2 4).', '.']",Figure 2. (a) Two entry wounds on the left side of the head seen on post-mortemCT reconstruction. (b) Photograph taken during autopsy.,yes
PMC4217540,Figure_3,oa_package/09/a1/PMC4217540.tar.gz,"['Macroscopically, the lungs of mice deficient for both IL-1 and IL-1 displayed pleural adhesions, large confluent nodules, similar to IL-1R1 or TNF deficient mice (A).', 'Microscopic investigation of the lungs of IL-1 plus IL-1 deficient mice revealed severe inflammation with important reduction of ventilated alveolar spaces, massive mononuclear cell and neutrophil infiltrations with extensive confluent necrosis and oedema, in the absence of proper granuloma formation at 35 days (B) and abundant mycobacteria within macrophages and in the extra-cellular space (', 'The lung lesions observed in the absence of both IL-1 and IL-1 were similar to those seen in mice deficient for IL-1R1 or TNF, although confluent necrosis was more pronounced in the absence of TNF 4 weeks post-infection (B).']","Figure 3 Presence of IL-1 or IL-1 prevents acute necrotic pneumonia in response to infection. Mice deficient for IL-1, IL-1, IL-1 plus IL-1, IL-1R1 or TNF and wild-type C57Bl/6 mice were exposed to H37Rv as in and macroscopic lung pathology assessed on day 35. Macroscopically, lungs of IL-1 plus IL-1 deficient mice showed large nodules similar to IL-1R1 deficient lungs (A). Lungs of TNF deficient mice with large, confluent nodules on day 28 post-infection are included for comparison. Microscopic examination showing extensive inflammation and necrosis in infected IL-1 plus IL-1, and in IL-1R1 deficient lungs (B; Hematoxylin and Eosin, magnification 50 for low power and 200 for details) with abundant mycobacteria in the extracellular space (C; Ziehl-Neelsen, magnification 50 for low power and 1000 for details). Bar graphs (D) summarise free alveolar space and scores of cell infiltration, necrosis and oedema at this time point ( = 811 mice per group from two independent experiments; * < 0.05; ** < 0.01; *** < 0.001, as compared to wild-type control).",yes
PMC6085636,Figure_1,oa_package/86/3b/PMC6085636.tar.gz,"[' 1a).', 'The phenotype and characteristics of PCOS patient-derived and non-PCOS patient-derived iPSCs.', 'Scale bars = 100 mThe proliferation speed of the PCOS patient-derived iPSCs was lower than that of the non-PCOS patient-derived iPSCs, the area of PCOS patient-derived iPSC colonies was obviously smaller than that of the non-PCOS patient-derived iPSC colonies, and the cell border of PCOS patient-derived iPSCs was not distinct compared with that of non-PCOS patient-derived iPSCs (', ' 1b and Additional file 2: S1A).', ' 1c).', ' 1d).', ' 1e).', ' 1f).', ' 1g).']","Fig. 1 The phenotype and characteristics of PCOS patient-derived and non-PCOS patient-derived iPSCs. Polycystic ovary syndrome (PCOS) disease modeling using induced pluripotent stem cell (iPSC) technology. After reprogramming, total RNA from PCOS patient-derived iPSCs was extracted for RNA microarray and quantitative real-time polymerase chain reaction (RT-PCR). Then, the mitochondrial functions of PCOS patient-derived iPSCs were measured using a respiration ability analyzer. The phase images of fibroblasts (HF) (scale bars=100 m) and iPSCs at day 2 (scale bars = 250 m) and day 6 (scale bars =100 m) after passage from PCOS patient-derived (PCOS1) and non-PCOS patient-derived iPSCs (non-P1). The cell border and surface area of iPSCs are different. iPSC colonies stained positive for OCT4, SOX2, and NANOG expression. Nuclei were stained with Hoechst 33,342 (blue). Fibroblasts (HF) were stained as negative control. Scale bars=25 m. Alkaline phosphatase (ALP) staining and immunofluorescence staining of SSEA-1. Fibroblasts (HF) were stained as negative control. Scale bars=100 m. Total and endogenous (Endo) expression levels of , , and by RT-PCR analysis in PCOS and non-PCOS patient-derived iPSCs, and HF. was the positive control. G-banding of PCOS and non-PCOS patient derived-iPSCs at passage 10 showed a normal karyotype. H&E staining of teratoma sections of PCOS and non-PCOS patient-derived iPSCs. Neural epithelium and melanocyte epithelium (ectoderm), cartilage (mesoderm), and gut epithelium (endoderm) are shown. Scale bars=100 m",yes
PMC4053290,Figure_1,oa_package/25/02/PMC4053290.tar.gz,['0-3424979369317542765(a) Chest CT scan before starting posaconazole showing reticulonodular opacities in the right upper lobe.'],Figure 1 (a) Chest CT scan before starting posaconazole showing reticulonodular opacities in the right upper lobe. (b) CT scan one month after starting posaconazole showing resolution of most of the opacities.,yes
PMC3132032,Figure_7,oa_package/9f/f2/PMC3132032.tar.gz,['The effect of intraarticular hyaluronan (HA) or saline on Safranin O histological staining of the osteoarthritis (OA) knee joint.'],"Figure 7 . Typical sections from the condyle and groove areas of the OA + HA group and the OA + saline group are shown. In each case, the boxed areas are shown at high magnification to the immediate right. Black arrows indicate cartilage loss.",yes
PMC11557046,Figure_2,oa_package/26/98/PMC11557046.tar.gz,['.'],"Figure 2. Comparison of baseline and follow-up outcome indicators. Comparison of (A) LVEDD and (B) LVEF between the 2 groups at baseline and after 8 weeks of treatment. Comparison of (C) 6-MWT between the 2 groups after 4 and 8 weeks of treatment. Comparison of (D) HAMA and (E) HAMD scores between the 2 groups at baseline and after 8 weeks of treatment. 6-MWT=6-minute walk test, HAMA=Hamilton Anxiety Scale, HAMD=Hamilton Depression Scale, LVEDD=left ventricular end-diastolic diameter, LVEF=left ventricular ejection fraction.",yes
PMC11526612,Figure_5,oa_package/99/90/PMC11526612.tar.gz,"[' 5A-B at the pharmacological treatment baseline (day 14) for saline and BLM 2.', ' 5A, green regions), and well-ventilated lungs (', ' 5B, green-blue regions).', ' 5A , yellow) matching large non-exchanging regions (', ' 5B , SVg = 0 ml/g; red).', ' 5A ), a marked escalation of hypo-aerated areas became apparent in the vehicle-treated mouse (BLM).', ' 5B ), the untreated mouse showed a very similar condition with respect to day 14, whereas a clear trend toward a functional recovery of poor SVg exchange regions, with the right lung showing an extended fully functional area, was observed in the NINT-treated mouse.', '\nPharmacological validation study: longitudinal densitometric and functional micro-CT derived biomarkers.', '05); the dotted line represents the threshold level utilized for the classification\nTo study the fibrosis development and the pharmaceutical effect of Nintedanib, we tracked changes from baseline to day 28 for control, vehicle, and treated groups, in term of MLAEXP, %Non-aerated, Median SVg and %Non ventilated (', ' 5C-F).', ' 5G-L).', ' 5C), indicating the presence of overall higher SVg values and a better ventilation in NINT-treated animals.']","Fig. 5 Pharmacological validation study: longitudinal densitometric and functional micro-CT derived biomarkers. ( ) Representative coronal micro-CT slices with overlaid aeration compartments at the commencement (day 14) of treatment for saline ( ) and vehicle (BLM, ) and at its conclusion (day 28) for vehicle (BLM, middle) and Nintedanib-treated (BLM+NINT, A bottom) mice. The aeration compartments are color-coded: green for Normo-aerated (Normo), yellow for Hypo-aerated (Hypo), and red for Non-aerated (Non). ( ) Maps of inspiratory-expiratory specific gas volume change (SVg=SVg -SVg ) for the same mice depicted in - . The range of display is from 0 to 1ml/g. ( ) Mean lung density at end-expiration (MLA ), ( ) percent extent of non-aerated compartment (%Non-aerated), ( ) Median SVg and ( ) percent extent of non-ventilated lung parenchyma (%Non-ventilation) were presented longitudinally with meanSEM at baseline, 7, 14, 21, and 28 days for saline, Vehicle and NINT groups. Statistical significance with respect to Vehicle was calculated by two-way ANOVA followed by Dunnetts t post-hoc test (* <0.05; ** <0.01; *** <0.001 vs. Vehicle group); statistical significance with respect to baseline was calculated by two-way ANOVA followed by idk post-hoc test (# <0.05; ## <0.01; ### <0.001 0d vs. baseline). ( - ) Same parameters of - were, also, presented as difference between end and start of the treatment (28d 14d) for Vehicle and NINT groups and reported as individual data and meanSEM for each group. Statistical significance is determined by Fishers exact test in order to compare Vehicle with NINT group (* <0.05); the dotted line represents the threshold level utilized for the classification",yes
PMC5679345,Figure_6,oa_package/ee/64/PMC5679345.tar.gz,"[' 6a).', ' 6a).', '', 'Inlets are 40 magnified images of the selected regions\nReduce parasite brain sequestration correlated with preserved BBB integrity, as evidenced by reduced vascular leakage of injected Evan s blue dye in rapamycin-treated mice (', ' 6b).', ' 6c).', ' 6c).']","Fig.6 Rapamycin reduces brain pathology. Luciferase activity and 18S expression indicative of luciferase-transgenic parasite accumulation in the brain of perfused mice (n=5/group) on day 6 of infection treated on day 4 with vehicle (V) or rapamycin (R, 5mg/kg) as indicated. Quantitation of Evans blue dye in brains and photographs of representative brains from mice (n=45/group) treated with vehicle or rapamycin (5mg/kg) on day 4 and injected with Evans blue dye on day 6 after infection to assess bloodbrain barrier function. Representative micrographs of brains from vehicle and rapamycin treated (5mg/kg) mice 6days after malaria infection. Upper panels show representative images of the thalamic area, which presents disperse micro and macro-haemorrhages (arrows) scattered throughout the parenchyma. It is also evident in this image the vascular leukostasis (Inset), caused by clustering of leukocytes and infected RBCs (arrow head) within the blood vessels. Mid panels show representative images of the hypothalamus region presenting leukostasis and scattered micro-haemorrhages (arrows), defined as haemorrhagic areas smaller than 2m (Inset). Lower panels show representative images comparing the cerebellum of vehicle and rapamycin treated mice. Inset shows a macro-haemorrhage in the molecular layer. Macro-haemorrhages (arrows) were defined as haemorrhages exceeding 2m . Quantitation of total number of brain haemorrhages per field of view are shown. Morphometric analysis showing the surface area of haemorrhages in whole brain sections are also shown. Hemorrhages were divided in micro-haemorrhages (<2m ) and macro-haemorrhages (>2m ). Data represent the meanSEM of over 100 fields of view. Scale bar=100m. Inlets are 40 magnified images of the selected regions",yes
PMC8303326,Figure_1,oa_package/71/8c/PMC8303326.tar.gz,"['2%) showed a pathological partial necrosis ().', '201615224127409564Small HCC in hepatic segment 3 at CT: before treatment, (a) arterial phase and (b) portal-venous phase, and 1 month after treatment with MW ablation (c) arterial phase, and (d) portal-venous phase: no enhancing tissue is depicted, and the response was classified as CR according to mRECIST.']","Figure 1 Small HCC in hepatic segment 3 at CT: before treatment, ( ) arterial phase and ( ) portal-venous phase, and 1 month after treatment with MW ablation ( ) arterial phase, and ( ) portal-venous phase: no enhancing tissue is depicted, and the response was classified as CR according to mRECIST. A complete pathological response was confirmed at pathology: ( ) HE staining (magnification 10).",yes
PMC9453305,Figure_2,oa_package/78/b7/PMC9453305.tar.gz,[],"Figure2 Gadoxetate disodium-enhanced MRI and histopathologic images of hepatocellular carcinoma (HCC) with different glypican-3 (GPC-3) expressions. A 72-year-old male patient with GPC-3 positive expression HCC , and a 77-year-old male patient with GPC-3 negative expression HCC . Pre-contrast T1-weighted images showed a hypointense lesion (3.54cm) in the right liver and a hypointense lesion (3.97cm) in the mid liver ; T2-weighted images showed hyperintense lesions with [ , blue arrow] and without [ , blue arrow] iron sparing in solid mass; diffusion-weighted images demonstrated presence [ , yellow arrow] and absence [ , yellow arrow] of marked diffusion restriction; arterial phase images showed nonperipheral-nonglobal arterial phase hyperenhancement [ , green arrow) and global arterial phase hyperenhancement [ , green arrow]; portal venous phase images showed nonperipheral washout and infiltrative appearance [ , orange and red arrow] and no-washout and smooth margin [ , orange and red arrow]; immunohistochemical staining revealed the GPC-3 positive and negative expressions.",yes
PMC10697830,Figure_3,oa_package/76/81/PMC10697830.tar.gz,[],"FIGURE 3 (A) There are dense perifollicular neutrophilic infiltrates with dense dermal infiltrate composed of lymphocytes, plasma cells, and abundant neutrophils, Hematoxylin and Eosin (H&E) stain, 100x. (B) Perifollicular collections of neutrophils (H&E), 400x. (C) The extruded hair fiber has stimulated granulomatous reaction, (H&E), 400x.",yes
PMC3228743,Figure_1,oa_package/49/9a/PMC3228743.tar.gz,['Imaging exams displaying the pulmonary aneurysm and associated features.'],"Figure 1 A) Chest radiograph (posteroanterior view) shows cardiomegaly, dilated main pulmonary artery and right pulmonary artery. B) Ecocardiography: paraesternal view, at great vessels level. Color-Doppler shows severe pulmonary regurgitation. C) Multislice contrast computed tomographic scan of the thorax on axial projection shows a close contact of the main pulmonary artery to the chest wall, dilated main pulmonary artery and both of its branches which have a fusiform morphology. D) On sagital projection the widest diameter of the aneurysm can be measured.",yes
PMC6292111,Figure_3,oa_package/31/f7/PMC6292111.tar.gz,"[' 3a).', ' 3b; red arrows) and in the distal ONL (', ' 3b, c; yellow arrows).', ' 3c; red arrow).', ' 3d), while in LPS-challenged-mice an amoebic-like morphology as signs of activation was observed (', ' 3e).', 'GCL, ganglion cell layer; IPL, inner plexiform layer; OPL, outer plexiform layer; RPE, retinal pigment epitheliumQuantification of microglia/macrophages with flow cytometryFlow cytometry was performed on the retinas of Cx3cr1gfp+/ mice treated with either LPS (1 mg/kg) or PBS for four consecutive days, approximately 6 h after the last LPS injection.']","Fig. 3 Activation and migration of retinal microglia/macrophages after LPS challenge in mice. In control retinas ( ), Iba-1-positive cells were mainly found in the inner and outer plexiform layer (IPL and OPL, respectively). In LPS-challenged retinas ( , ), Iba-1-positive microglia/macrophages were also detected in the proximity of the sub-retinal fluid accumulation (red arrows in ) and in the distal ONL (yellow arrows in and ). Iba-1-positive cells migrating towards the outer retina were also detected (red arrow in ). In control retinal whole mounts, GFP-positive microglia cells have a ramified morphology typical for resting microglia ( ). After LPS challenge, activated GFP-positive cells with retracted processes and round cytosol were detected in the retina ( ). One representative retina out of 4 is presented for each time point. Scale bars: 100m. GCL, ganglion cell layer; IPL, inner plexiform layer; OPL, outer plexiform layer; RPE, retinal pigment epithelium",yes
PMC3474400,Figure_1,oa_package/f3/3b/PMC3474400.tar.gz,"['A 2-cm incision was made at the bilateral inguinal area and the inguinal fat pad was exposed ().', 'phosphatidylcholine and organic silicium: a pilot studyJ Cosmet Dermatol20076250257180476106PereiraLHSterodimasACorrection for the iatrogenic form of banana fold and sensuous triangle deformityAesthetic Plast Surg200832923927186635137BecharaFGSkryganMKreuterACytokine mRNA levels in human fat tissue after injection lipolysis with phosphatidylcholine and deoxycholateArch Dermatol Res2008300455459185634228RittesPGRittesJCCarriel AmaryMFInjection of phosphatidylcholine in fat tissue: experimental study of local action in rabbitsAesthetic Plast Surg200630474478168586609SallesAGVallerCSFerreiraMCHistologic response to injected phosphatidylcholine in fat tissue: experimental study in a new rabbit modelAesthetic Plast Surg2006304794841685588910TreacyPJGoldbergDJUse of phosphatidylcholine for the correction of lower lid bulging due to prominent fat padsJ Cosmet Laser Ther200681291321697136111SaltiGGhersetichITantussiFPhosphatidylcholine and sodium deoxycholate in the treatment of localized fat: a double-blind, randomized studyDermatol Surg20083460661805304912DuncanDIPalmerMFat reduction using phosphatidylcholine/sodium deoxycholate injections: standard of practiceAesthetic Plast Surg2008328588721861268013RittesPGThe use of phosphatidylcholine for correction of localized fat depositsAesthetic Plast Surg2003273153181505855714HasengschwandtnerFInjection lipolysis for effective reduction of localized fat in place of minor surgical lipoplastyAesthet Surg J2006261251301933889215HexselDSerraMMazzucoRPhosphatidylcholine in the treatment of localized fatJ Drugs Dermatol200325115181455839916BecharaFGSandMHoffmannKFat tissue after lipolysis of lipomas: a histopathological and immunohistochemical studyJ Cutan Pathol2007345525571757633417Yagima OdoMECuceLCOdoLMAction of sodium deoxycholate on subcutaneous human tissue: local and systemic effectsDermatol Surg2007331781881730060318RotundaAMAblonGKolodneyMSLipomas treated with subcutaneous deoxycholate injectionsJ Am Acad Dermatol2005539739781631005719Schuller-PetrovicSWolkartGHoflerGTissue-toxic effects of phosphatidylcholine/deoxycholate after subcutaneous injection for fat dissolution in rats and a human volunteerDermatol Surg2008345295421837098020RhaEYKangJALeeJHComparative analysis about the effect of isolated phosphatidylcholine and sodium deoxycholate for the viability of adipocyteJ Korean Soc Plast Reconstr Surg201037531534Inguinal fat pad of a ratElevation of pedicled inguinal fat pad of a rat.']",Fig. 1 Inguinal fat pad of a rat Elevation of pedicled inguinal fat pad of a rat.,yes
PMC4238966,Figure_2,oa_package/25/52/PMC4238966.tar.gz,[],"FIGURE 2 EFhd2 protein levels are not altered in the hippocampi of human tauopathy patients. Western blots showing EFhd2 protein levels in human hippocampal tissue RIPA buffer extracts from nondemented controls and individuals with Alzheimer disease (AD), Pick disease (Picks), and frontotemporal dementia with tau mutations (FTLD-tau). Western blot were repeated 3 times with a 293T-cell line lysate loaded as a calibrator on each gel. Beta-actin was used as a loading control. Western blot analysis of total and phosphorylated tau protein in human frontal cortex samples. Quantification of EFhd2 protein bands by densitometry, n = 3 for each sample. Error bars = SEM. Scatter plots of densitometry values for EFhd2 against values for each of the phosphorylated Tau antigens (PHF-1, CP-13, RZ3). Linear regression was done in GraphPad prism and showed no correlation between EFhd2 and any of the phospho-tau antigens.",yes
PMC9273760,Figure_2,oa_package/bb/b0/PMC9273760.tar.gz,"['2A).', '2A).', '2B, C), but also present in some images generated with CLIP guidance (', '2D).', '2E).', 'Systematic evaluation of style and content in GLIDE-generated imagesHow good is GLIDE in generating a correct style and correct content for medicine-related text prompts?']","Fig. 2 Common confusions of the model. Example images of common confusions observed in our study, Generated images without and with CLIP guidance for four text prompts. For each prompt, eight random images are shown and these images are not cherry-picked.",yes
PMC11563190,Figure_3,oa_package/7c/e2/PMC11563190.tar.gz,['CT of the thorax with contrast sagittal view displaying tracheal deviation before thoracentesis.'],Figure 3 CT of the thorax with contrast sagittal view displaying tracheal deviation before thoracentesis.,yes
PMC6269335,Figure_13,oa_package/0c/9a/PMC6269335.tar.gz,[],"Fig. 13 Age-related changes in fibrous dysplasia (FD). CT of the head on the same patient at age of 6 ( ), 7 ( ) and 14 years ( ). Diffuse FD involvement with homogenous ground glass appearance ( ), which demonstrates the tendency to develop cystic lesions and become more heterogeneous with time ( )",yes
PMC10382492,Figure_3,oa_package/fe/a4/PMC10382492.tar.gz,"['3, Table 1).', 'The expression and function of H19 in UFs and endometriosis.', 'H19 and diminished ovarian reserveThe number of follicles and oocytes (the ovarian reserves) decreases with age [47], resulting in decreased female fecundity and infertility.']","Fig. 3 The expression and function of H19 in UFs and endometriosis. H19 regulates ECM deposition via Let7/TET3/TGFR2 and TSP1/TGF- pathways in UFs. H19 regulates cell proliferation via Let7/HMGA2 and Let7/TET3/MED2 pathways in UFs. H19 regulates cell proliferation via Let7/IGF1R in endometriosis. H19 modulates cell proliferation via miR-124-3p/ITG3 in endometriosis. H19 modulates cell proliferation via miR-216a-59/ACTA2 in endometriosis. H19 affects cell proliferation via miR-342-3p/IER3 in endometriosis. ACTA2 actin alpha 2, ECM extracellular matrix, ESCs endometrial stromal cells, HMGA2 high mobility group AT-hook 2, IER3 immediate early response 3, IGF1R insulin-like growth factor 1 receptor; ITG3 integrin beta 3, lncRNA long non-coding RNA, miR- microRNA, MED12 mediator complex subunit 12, TET3 Ten eleven translocation 3, Th17 T helper cell 17, TGF- transforming growth factor-beta, TGFR2 transforming growth factor beta-receptor 2, TSP1 thrombospondin-1, UFs uterine fibroids.",yes
PMC5079195,Figure_2,oa_package/e7/f2/PMC5079195.tar.gz,"['18 The PK Papyrus (Biiotronik, Berlin, Germany) is a sixth generation BMS of cobalt-chromium alloy, thinner struts of which allow exceptional flexibility and deliverability, even in challenging vessels ().', 'Bailout of Ellis type III perforation in left circumflex (LCx) artery CTO intervention.']","Fig. 2 Bailout of Ellis type III perforation in left circumflex (LCx) artery CTO intervention. (A) Type III perforation in distal LCx after in-stent balloon dilatation. (B) Deployment of PK Papyrus (Biotronik, Berlin, Germany) covered stent after advancement of Graftmaster stent has failed. (C) Final result.",yes
PMC8540642,Figure_2,oa_package/35/eb/PMC8540642.tar.gz,"['05, Dunnett; A).', 'At the end of treatments, mice were euthanized for tumor lesions recovery and to perform a macroscopic analysis of the developed lesions (C).', 'Similar characteristics in all tumors were present in the treated groups with an ovoid or smooth-surfaced morphology and the presence of vascularity (C).', 'However, minor differences in the color of tumors were found: pink for AE and whitish for AF02; groups treated with carboplatin and vehicle presented a yellow tumor with a nodular surface (C).', 'These results correlate with the tumor volume of lesions present in rodents along with the treatment time (B).', '05, Dunnett; B,C).', 'Antineoplastic activity of AE and AF02 from RHTR in ovarian cancer.']","Figure 2 Antineoplastic activity of AE and AF02 from RHTR in ovarian cancer. The bodyweight of rodents was monitored with an electronic bascule for 28 days ( ). The tumor volume was determined with a Vernier caliper in mice treated with AE and AF02 at 200 mg/kg/i.p./day (Tumoral volume = [Larger diameter (Shorter diameter) ]/2) ( ). Morphological changes in tumor lesions were analyzed at the end of treatments, and the percent inhibition was calculated ( ). Results show the mean SD of two biological replicates ( = 5). * 0.05 vs. the control group without treatment (1 PBS) (ANOVA). The positive control was carboplatin (50 mg/kg/i.p./3 alternating days per week in mice). RHTR, ; AE, aqueous extract; AF02, aqueous fraction-02 (active fraction obtained with ethyl acetate).",yes
PMC9777900,Figure_4,oa_package/80/2d/PMC9777900.tar.gz,"['The evolution remained favorable until the next evaluation in 2022, without treatment with oral corticosteroids ().', 'Chest X-ray 2022 Case 1.']","Figure 4 Chest X-ray2022Case 1. Diffuse reticular opacities, especially in the basal regions.",yes
PMC10788162,Figure_4,oa_package/4e/06/PMC10788162.tar.gz,['There was\nimmediate detachment of the germinal layer in all cysts during aspiration as shown in ('],"Fig. 4. ( ) Detachment of the germinal layer (arrow) in cyst (C) during aspirationof clear non-turbid fluid of hepatic cystic lesions from female dromedary camel.( ) Ultrasound-guided aspirated clear non-turbid fluid of hepatic cysticlesions. Ds, dorsal; Vt, ventral.",yes
PMC8316995,Figure_5,oa_package/b2/8c/PMC8316995.tar.gz,[],"Figure5 Association of CXCR4 expression in T cells, CD4 and CD8 T cells with aGVHD in haploidentical HCT recipients. The proportions of CXCR4 T cells , CXCR4 CD4 T cells , and CXCR4 CD8 T cells were detected by flow cytometry in recipients with grade 0-I and II-IV aGVHD after haploHCT. values are shown on the graphs.",yes
PMC5398743,Figure_9,oa_package/cc/1f/PMC5398743.tar.gz,"['14 However, this receptor is greatly upregulated in vasoproliferative stage of OIR in forming intra-retinal as well as extra-retinal neovascularization ().', '0Effect of anti-KDR on dog OIR.']","Figure 9 KDR (VEGFR2) localizationin the canine OIR model. Sections from a 15-day-old oxygen-treated animal immunostained with anti-vWf ( and ), anti-KDR (VEGFR2) antibody that was preincubated overnight with phosphate buffered saline ( and ), or anti-KDR antibody preincubated overnight with 10 molar excess soluble KDR (sVEGFR2) ( and ). Shown are the areas from the border of vascularized retina ( ) and a more posterior region with intravitreal neovascularization ( ). Preincubation of antibody with soluble KDR completely eliminated both retinal vascular and intravitreal neovascular immunostaining ( and ). Double arrows, amino ethyl carbazol (AEC) reaction product in all; original magnification 50. Copyright 2002 Association for Research in Vision and Ophthalmology. Reproduced from McLeod DS, Taomoto M, Cao J, Zhu Z, Witte L, Lutty GA. Localization of VEGF receptor-2 (KDR/Flk-1) and effects of blocking it in oxygen-induced retinopathy. . 2002;43:474482. vWf, von Willebrands factor; KDR, kinase domain receptor; NV, neovascularization.",yes
PMC8544559,Figure_2,oa_package/19/5b/PMC8544559.tar.gz,"['There were no other suspicious pelvic mass lesions or lymphadenopathy noted ().', 'Imaging findings from MRI of pelvis.']","Figure 2 Imaging findings from MRI of pelvis. ( ) Axial T2 image of the pelvis shows a heterogenous, well circumscribed T2 hyperintense clitoral mass with T2 hypointense margin with internal low signal (yellow arrows). ( ) On axial T1, the lesion is hypointense, typical of most malignancies (yellow arrows). ( ) On axial diffusion weighted imaging (DWI) with B = 800, the clitoral mass is bright (restricting) suggesting high cellularity (yellow arrows). ( ) On axial dynamic T1 post contrast, the clitoral mass progressively enhances.",yes
PMC11411375,Figure_5,oa_package/b1/cf/PMC11411375.tar.gz,"['Finally, the allograft tension band augmentation is completed by fixating the remaining allograft free ends projecting through the medial and lateral margins of healthy remaining patellar tendon back proximally to the inferior pole of the patella ().', 'A knotless knee FiberTak (Arthrex) anchor is placed at the inferior pole of the patella, with care taken to avoid the previously made and filled socket (A, Table 1).', '0 FiberWire (Arthrex) repair suture preloaded into the FiberTak anchor is passed through the two free limbs of the graft in a mattress fashion (B).', 'The free end of the repair suture is passed through the anchor using the shuttling suture and then tensioned to reduce the graft to the inferior pole of the patella in an onlay fashion (C).', 'The excess graft is excised (D).', 'Right knee clinical photographs demonstrating fixation of the remaining allograft to the inferior pole of the patella using a knotless knee FiberTak anchor.']","Fig 5 Right knee clinical photographs demonstrating fixation of the remaining allograft to the inferior pole of the patella using a knotless knee FiberTak anchor. (A) Drilling for the anchor, with care taken to avoid the previously made and filled socket. (B) The repair suture preloaded into the FiberTak anchor is passed through the 2 free limbs of the graft in a mattress fashion. (C) The free end of the repair suture is passed through the anchor using the shuttling suture and then tensioned to reduce the graft to the inferior pole of the patella in an onlay fashion. (D) The excess graft is excised. Key: () 2 limbed semitendinosus allograft, () FiberTape for InternalBrace creation, () remaining healthy proximal patellar tendon.",yes
PMC7640704,Figure_2,oa_package/3d/9c/PMC7640704.tar.gz,"['2).', 'Cellular infiltration after TBI.', 'Upregulation of adhesion molecules in cerebral vessels and production of chemokines by activated microglia and astrocytes finally cause blood leukocytes to migrate into the brain parenchyma where monocytes are suggested to cause additional damage to the brainMeningesDuring inflammation, the entry of immune cells to the CNS parenchyma is secondary to their infiltration into the meninges [91].']","Fig. 2 Cellular infiltration after TBI. In healthy brain, the functional unit constituted by firmly coupled endothelial cells and astrocytic end-feet frame the blood-brain barrier (BBB). Immune cells flow unreservedly in the blood vessel, and in the brain parenchyma, blood-borne and brain-borne proteins cannot pass into the other compartment, resting microglia survey the intact brain ecosystem. Following TBI, the BBB disrupted/leaked activating the endothelial cells. The tight junctions between endothelial cells perish. This allows immune cells to adhere at the blood vessels lamina and then transmigration to the brain parenchyma. Specific brain proteins (e.g. S100B) are released into the blood according to their concentration gradient in exchange, serum protein enters the brain parenchyma which has been demonstrated in the cartoon. Now, microglia switch from their resting state to an activated state, embracing a phagocytic phenotype and secreting pro-inflammatory proteins. Upregulation of adhesion molecules in cerebral vessels and production of chemokines by activated microglia and astrocytes finally cause blood leukocytes to migrate into the brain parenchyma where monocytes are suggested to cause additional damage to the brain",yes
PMC4141340,Figure_19,oa_package/66/8b/PMC4141340.tar.gz,[],Fig. 19 Sub peritoneal pelvic adenomucinosis presenting as slow growing pelvic mass 6years after surgery for appendiceal mucocele. Axial contrast CT reveals retrorectal multiloculated cystic lesion ( ) with calcification ( ). Axial T2-weighted MRI and sagittal T2-weighted MRI reveal a cystic lesion ( ) with multiple septations. Axial contrast T1-weighted MRI reveals peripheral and septal enhancement of the lesion ( ),yes
PMC10625271,Figure_6,oa_package/69/aa/PMC10625271.tar.gz,"['6A).', '6B).', '6C).', '6D G).', '6H), demonstrating that downregulated TIM partially contributes to the TRF-mediated inhibition of tumor cell proliferation and migration.', 'TIM contributes to TRF-dependent changes in tumor suppression in vitro and in vivo.', 'Data was expressed as mean SD for A H and mean SEM for ', '0001Finally, to examine whether this inhibitory effect of TRF on tumors is mediated by TIM in vivo, a mouse xenograft model was established by implanting 1 107 stable TIM-overexpressing A549 cells into the back subcutaneous tissue of nude mice, and tumor tissues were harvested at the T0, T8, and T16 time points.', '6I, J).', '6K), as illustrated by reductions in final tumor weight and volume (', '6L, M).']","Fig. 6 TIM contributes to TRF-dependent changes in tumor suppression in vitro and in vivo. The protein expression of TIM in stable TIM-overexpressing A549 cells. Left: representative blots; right: quantitative results. Expression relative to OE-control group at T0. EdU proliferation assay in stable TIM-overexpressing A549 cells. Left: representative images (scale bar=100 m); right: quantitative results ( =3). Colony formation proliferation assay. Left: representative images; right: quantitative results ( =3). Representative images of a flow cytometry assay to detect the cell cycle (PI staining) and cell apoptosis (Annexin V staining for early apoptosis and DAPI staining for late apoptosis) in stable TIM-overexpressing A549 cells. Quantification of the cell cycle and cell apoptosis ( =3). Wound healing migration assay. Left: representative images (scale bar=100 m); right: quantitative results ( =3). TIM expression of the xenograft model mice inoculated with stable TIM-overexpressing A549 cells detected by immunohistochemistry; representative images (scale bar=50 m) and quantification result ( =3). Photograph of dissected tumors from xenograft model mice injected with TIM-overexpressing A549 cells with or without TRF. Tumor volume ( =1112 mice per group). Tumor weight ( =1112 mice per group). Histopathology. Representative PCNA and Ki67 staining of tumor tissue (scale bar=50 m). Quantification of the PCNA-positive staining and Ki67-positive staining ( =8 in each group). OE-Ctrl, negative-overexpression-control; OE-TRF, negative-overexpression-control with TRF; OE-TIM, TIM-overexpression; and OE-TIM+TRF, TIM-overexpression with TRF. Data were analyzed by one-way or two-way ANOVA with Tukeys post hoc test. Data was expressed as meanSD for Fig. 6AH and meanSEM for Fig. 6 JP. * <0.05; ** <0.01; *** <0.001; **** <0.0001",yes
PMC6766113,Figure_2,oa_package/81/c5/PMC6766113.tar.gz,"['A new abdominal MRI was performed at day 9 of life and depicted an enlargement of the lesion (37 32 70 mm), while bone scan and 123I-MIBG scan were normal (see s 2(a) and 2(b)).', '001""/>Postnatal MRI depicts the enlargement of the lesion.']",Figure 2 Postnatal MRI depicts the enlargement of the lesion.,yes
PMC9510713,Figure_6,oa_package/b1/93/PMC9510713.tar.gz,[],"FIGURE 6 Subcellular localization of PRMT1 and PRMT6 constructs transfected in C2C12 mouse myoblast cells in the absence and presence of MeCP2. Scale bar 5m. Boxplots depict the heterochromatin clustering in C2C12 cells without and with low and high levels of nuclear PRMT1 and 6 represented by the number and size of the heterochromatin clusters obtained from high-content screening microscopy. Two biological replicates, statistical significance calculated using Wilcoxon-Rank test. * < 0.05, ** < 0.005, *** < 0.001, n. s Not significant. -values and n-values are summarized in .",yes
PMC9692088,Figure_1,oa_package/d6/38/PMC9692088.tar.gz,"['These data are therefore suggestive of the pathological propagation in the classical CLN1 disease from the periphery, via the spinal interneurons, trough ascending spinal tracts, to the brainstem, and then into the cerebral hemispheres, cerebellum and retina ().', '(A) The Bottom-up propagation in CLN1 disease via the peripheral sensory nerves, the dorsal root ganglia and via the spinal interneurons to the brainstem (all within the initial 1 1 years of life), and then into brainstem, the cerebral hemispheres, and, at 2 3 years of age, affecting the cerebellum and retina.']","Figure 1 The Bottom-up propagation in CLN1 disease the peripheral sensory nerves, the dorsal root ganglia and the spinal interneurons to the brainstem (all within the initial 11 years of life), and then into brainstem, the cerebral hemispheres, and, at 23 years of age, affecting the cerebellum and retina. The Top-down disease propagation in CLN3 starting at the outer retina 57 years of age, followed by cerebral and cerebellar atrophy in early and late adolescence, respectively, with the extrapyramidal system affected in between. Affection of the autonomic nerve supply of the heart, the sinus node and the heart conduction system takes place in late adolescence, whereas impact of the peripheral motor nerves occurs in late adolescents/early twenties.",yes
PMC5519492,Figure_5,oa_package/b1/49/PMC5519492.tar.gz,"[', 2013); however, the AD prevalence was considerably lower in ALS patients aged 75 years and above ( 5A).', 'Notably, cognitive evaluation revealed a subtle cognitive dysfunction in non-AD ALS patients older than 75 years, despite excluding patients with over-lapping FTLD ( 5B).', ' 5Prevalence of AD in ALS/FTLD Patients Is Significantly Lower than in Healthy Controls(A) Prevalence of AD in ALS patients compared to expected prevalence in a normal age-matched population.', 'However, similar to how AD prevalence was lower in ALS patients who were 75 years or older, A pathology was significantly reduced in the brains collected from ALS/FTLD-TDP patients 75 years or older, compared to the age-matched controls (s 5C and 5D).', 'Importantly, we found that CD68 burden was significantly higher in ALS patients with TDP-43 pathology as compared to ALS patients without TDP-43 inclusions, or to healthy controls (s 5E and 5F; Table S2), supporting a critical role for TDP-43 in regulating microglial function.', 'In the MND cases, but not in the controls, we could observe examples of cytoplasmic inclusions positively stained for phospho-TDP-43 (pTDP-43) within microglial cells positive for Iba1 ( 5G), indicating that microglial TDP-43 can contribute to TDP-43 pathology.']","Figure5 Prevalence of AD in ALS/FTLD Patients Is Significantly Lower than in Healthy Controls (A) Prevalence of AD in ALS patients compared to expected prevalence in a normal age-matched population. ALS cohort was divided into two sub-groups according to their age at the time of AD screening: 6574 and 75 years. The expected prevalence of AD in these age groups is 3% and 17%, respectively. AD prevalence is 4.7% in ALS patients aged 6574 years but increases to only 7.1% in those 75 years. (B) MiniMental State Examination (MMSE) scores reported from ALS patients with or without AD, indicate a mild cognitive impairment in ALS patients above 75, despite no AD. (C) Representative images of beta-amyloid load in Control, ALS, FTLD, and AD biopsies in 6574 and 75 years cases. (D) Amyloid burden quantified according to the Thal A phase (TAP) scoring system, in 6574 and 75 years controls, ALS, FTLD, and AD cases indicates decreased A in ALS and FTLD cases 75 years (mean SEM, Control n= 40, ALS n= 35, FTLD n= 25, AD n= 62, p< 0.05, p< 0.0001, by one-way ANOVA of unpaired t test, followed by Holm-Sidaks multiple comparisons test). (E and F) Representative micrographs for immunostaining against CD68 in cortical section of healthy control, and ALS cases negative and positive for TDP-43 pathology, respectively (E). Relative quantification of CD68 burden in ALS patients with and without TDP-43 pathology indicates increased burden in ALS patients with TDP-43 pathology (F) (mean SEM, Control n= 6; p< 0.05, ALS TDP-43 negative (n= 11) versus ALS TDP-43 positive (n= 16), by using two-tailed unpaired ttest); scale bar: 50m. (G) Representative confocal z stack and orthogonal projections of microglial cells positive for Iba1 and pTDP-43 markers, in human post-mortem cortical sections of MND cases; scale bar: 20m; scale bar for the orthogonal projection: 40m.",yes
PMC4132577,Figure_3,oa_package/4d/34/PMC4132577.tar.gz,['Protein levels in hippocampus and cerebellum of CCL2-tg and non-tg mice at different ages determined by Western blot.'],Figure 3 . Mean (SEM) values for levels of housekeeping proteins. Mean (SEM) values for levels of signal transduction proteins. Mean (SEM) values for levels of synaptic proteins. Representative Western blots are shown above the respective bar. Top blot is the protein indicated for the graph; bottom blot is actin in the same lane. Numbers in bars are the number of animals measured. * = statistically significant difference between CCL2-tg and non-tg for the age group.,yes
PMC5342480,Figure_1,oa_package/ee/be/PMC5342480.tar.gz,"[').', 'SIRT3 negatively regulates fibrotic responsesA.']","Figure 1 SIRT3 negatively regulates fibrotic responses - Normal adult lung fibroblasts transfected with SIRT3 or empty vector were incubated with TGF-2 (10 ng/ml) for 24 h. A. Results of real-time qPCR normalized with are means SD of triplicate determinations from an experiment representative of three. * < 0.05. Whole cell lysates were examined by Western analysis. Representative blots from an experiment representative of three; -fold change in band intensities normalized with tubulin shown below. Cgn I, Type I collagen. Fibroblasts were co-transfected [SBE] -luc along with SIRT3 or empty vector. Whole cell lysates were analyzed for their luciferase activities. Results are the means SD of triplicate experiments. * < 0.05. Fibroblasts were transiently transfected with siRNA or scrambled (control) siRNA and incubated for 48 h. Results of qRT-PCR normalized with are means SD of triplicate determinations from an experiment representative of three. * < 0.05.",yes
PMC5463301,Figure_3,oa_package/aa/84/PMC5463301.tar.gz,"['3).', '\nAngiostrongylus vasorum from dogs: a) details of anterior extremity of an adult worm showing esophagus, scale bar 100 m; b) details of adult female caudal extremity, presenting vulva (black arrowhead) and anus (white arrowhead), scale bar 100 m; c) male caudal extremity showing the copulatory bursa with spicules, scale bar 100 m; d) Baermann test: first-stage larva of A.', 'vasorum, scale bar 20 m; e) numerous larvae recovered from the bronchoalveolar lavage (BAL) incorporated in strands of mucus, scale bar 50 m; f) eggs containing a coiled larva, recovered from BAL, scale bar 20 m\nThe entire archive of the Veterinary Pathology Laboratories at Veterinary School of Milan University (VPL) was browsed through 1998 2016 to collect data on diagnosed A.']","Fig. 3 from dogs: ) details of anterior extremity of an adult worm showing esophagus, scale bar 100m; ) details of adult female caudal extremity, presenting vulva (black arrowhead) and anus (white arrowhead), scale bar 100m; ) male caudal extremity showing the copulatory bursa with spicules, scale bar 100m; ) Baermann test: first-stage larva of , scale bar 20m; ) numerous larvae recovered from the bronchoalveolar lavage (BAL) incorporated in strands of mucus, scale bar 50m; ) eggs containing a coiled larva, recovered from BAL, scale bar 20m",yes
PMC7394136,Figure_4,oa_package/e4/0b/PMC7394136.tar.gz,"['The purulent material drained tested negative for organisms, amoebic and hydatid disease ().', 'Axial view of arterial phase of CT scan of the abdomen cholecystitis with pneumobilia with resolving liver abscesses with percutaneous pigtail catheter in situ.']",Figure 4 Axial view of arterial phase of CT scan of the abdomen cholecystitis with pneumobilia with resolving liver abscesses with percutaneous pigtail catheter .,yes
PMC10668929,Figure_21,oa_package/51/da/PMC10668929.tar.gz,[],Fig. 21. Normal ischiofemoral space. Graphic illustration ( ) demonstrates normal transducer position for assessing the ischiofemoral space. Short-axis US image ( ) shows the normal sonographic appearance of the space (qf quadratus femoris; arrowhead sciatic nerve) is modified with permission from Flores et al.,yes
PMC3835636,Figure_2,oa_package/90/19/PMC3835636.tar.gz,"['0001) (Table 4, ).', 'RFA = radiofrequency ablation, FLL = focal liver lesion, F/U = follow-upSurgically proven liver metastasis from ascending colon cancer in 53-year-old woman.']","Fig. 2 Surgically proven liver metastasis from ascending colon cancer in 53-year-old woman. Contrast-enhanced CT scans on arterial and portal phase show no definite liver metastasis. Respiratory-triggered, T2-weighted FSE image reveals one metastatic lesion in gallbladder bed (arrow). Arterial and equilibrium phase images after administration of gadoxetic-acid show lesion as faintly hypo-intense nodule (arrow). Gadoxetic-acid HBP MR image shows low-signal-intensity nodule in segment IV of liver (arrow). FSE = fast spin echo, HBP = hepatobiliary-phase",yes
PMC7532565,Figure_1,oa_package/01/f3/PMC7532565.tar.gz,"[' 1a), regardless of the cohort tested.', ' 1b): the values recorded in the AD population were significantly higher than in the NAD group regardless of analytical method, the sex, or the age as covariate.', ' 1c).', 'Values of A 40, A 42, tau, and p-tau (181) are in picograms per milliliterCSF A 42 and A 40 in non-AD and AD populations.', 'Note that A has been assessed using five different detection methods (supTable 1)Meta-analysis including the four independent cohorts.', 'pdf"">Additional file 3 : Sup-.']","Fig. 1 CSF A42 and A40 in non-AD and AD populations CSF concentration of A42 ( ) and A40 ( ) in four independent cohorts (Montpellier 1 (Mtp-1), Montpellier 2 (Mtp-2), Paris, SPIN-Barcelona) confirmed the significant difference between NAD and AD patients for both analytes ( test). Differences in CSF A40 measured in two ADNI cohorts ( ) were also significant between ADNI(+) and ADNI() patients stratified using the PLM scale (see the section). Note that A has been assessed using five different detection methods (supTable )",yes
PMC6749875,Figure_10,oa_package/67/6a/PMC6749875.tar.gz,[],Fig. 10 Upper and lower lip projections,yes
PMC11628164,Figure_1,oa_package/a5/87/PMC11628164.tar.gz,['Extraoral views.'],Figure 1 Extraoral views. a: Front view of the patient showing facial asymmetry due to left cheek and para-nasal swelling. b: Top view of the patient showing facial asymmetry. The black arrows show the facial asymmetry.,yes
PMC3367995,Figure_2,oa_package/51/8e/PMC3367995.tar.gz,"['H1N1pdm infection caused severe disease in mice of all three groups (A).', 'g002Severe A/Mexico/4108/2009 (H1N1pdm) infection triggered increased IL-6 expression in C57BL/6J, BALB/cJ, and B6129SF2/J mice.', 'At both day 3 and 5 pi, Il6 mRNA was significantly increased in the lungs of all mouse strains following H1N1pdm infection (B) peaking on day 3 pi.', 'Dramatic increases in IL-6 cytokine levels were observed at day 5 pi (C).']",10.1371/journal.pone.0038214.g002,yes
PMC5783844,Figure_7,oa_package/33/be/PMC5783844.tar.gz,"['The chronotropic response of extracellular electrograms recorded from control, SCO2E140K and SCO2G193S iPSC CMs to isoproterenol.']","Figure 7 The chronotropic response of extracellular electrograms recorded from control, SCO2 and SCO2 iPSCCMs to isoproterenol. ( ) The spontaneous beating rate of control ( =11), SCO2 ( =7) and SCO2 ( =10) iPSCCMs. ( ) The effect of isoproterenol on the beat rate of iPSCCMs and the blocking effect of the blocker metoprolol. ( ) The EB was perfused initially with DMEM solution (Control) and then with isoproterenol 10 M in DMEM solution. The different time intervals denote the arrhythmia in the isoproterenoltreated SCO2mutated EBs. The arrhythmia was blocked by the blocker metoprolol. ( ) Control, ( ) SCO2 and ( ) SCO2 iPSCCMs. Results are expressed as per cent change from control (absence of isoproterenol). The arrhythmia is marked by red bar; bpm: beats per minute; C: control conditionsDMEM solution in the absence of isoproterenol; Iso: Isoproterenol; Met: Metoprolol; * <0.05, ** <0.001 ( control).",yes
PMC5585498,Figure_5,oa_package/7a/2e/PMC5585498.tar.gz,['Clinical findings.'],Figure 5 Clinical findings. An ulceration covered with whitish pseudomembrane located on the left floor of the mouth (A) and multiple lymphadenopathies of the submental and submandibular regions (arrow in B) are shown.,yes
PMC4041651,Figure_3,oa_package/61/bc/PMC4041651.tar.gz,"['The collagen volume fraction was reduced significantly in the TAC+TL and TAC+TH groups compared with the TAC group (A and 3B, P 0.', 'Failing hearts had mitochondrial morphological alteration and disorganized cristae, and disorganized Z-line structures (C).', 'In contrast to the TAC group, mitochondrial morphology and Z-line structures were nearly normal in the TXL-treated groups (C).', 'g003TXL reduces cardiac fibrosis and ameliorates myocardial ultrastructure derangement after TAC.']",10.1371/journal.pone.0098047.g003,yes
PMC8509850,Figure_6,oa_package/54/f1/PMC8509850.tar.gz,"['We found that both ribbon-tethered vesicles as well as vesicles docked to the active zone were decreased in number in MOG/CFA-injected mice in comparison to CFA-injected control mice (A,B; for quantification, C F).', 'In contrast the number of synaptic vesicles in the cytosol (reserve pool) of rod photoreceptor synapses was found to be unchanged between MOG/CFA-injected EAE mice and CFA-injected littermate control mice (G,H).', 'TEM images at 48,000 magnification were also used to count the number of docked vesicles and synaptic vesicles tethered to the synaptic ribbon and for determining the synaptic vesicle density in cytosol ().', 'The number of docked synaptic vesicles at the active zone within 150 nm vicinity were counted from the TEM images of rod photoreceptor synapse in CFA control and MOG/CFA immunized mice, as previously described in [30] ().', 'The number of docked vesicles and ribbon-tethered vesicles are reduced in early EAE mice in comparison to control mice.']","Figure 6 The number of docked vesicles and ribbon-tethered vesicles are reduced in early EAE mice in comparison to control mice. ( ) Representative TEM images of rod photoreceptor synapses from CFA-injected control mice ( , ) and MOG/CFA-injected EAE mice ( , ). The yellow lines in ( , ) denote the 150 nm range in vicinity of the active zone in which the docked vesicles (white circles) were counted. The red spheres in ( , ) exemplify the ribbon-tethered synaptic vesicles. White arrows in ( ) point to the arciform density. ( , ) Average number of docked vesicles (CFA: 5.5 0.14 vesicles; MOG/CFA: 3.1 0.10 vesicles). ( , ) Average number of ribbon tethered vesicles (CFA: 12.96 0.26 vesicles; MOG/CFA: 8.93 0.17 vesicles). ( , ) Average number of cytosolic vesicles determined in a square area of 200 200 nm within the presynaptic terminal in distance from the synaptic ribbon (CFA: 12.85 0.18 vesicles; MOG/CFA: 12.83 0.12 vesicles). Values in ( , , ) are means S.E.M. In the box-and-whiskers plots ( , , ) of the data from ( , , ), mean values are indicated by solid horizontal lines; median values by dotted horizontal lines. Boxes represent the 25th75th percentiles of data points and whiskers are equal to 1.5 times of the interquartile range (IQR). MannWhitney U-test was used to determine the statistical significance. Abbreviations: ho, horizontal cell dendrite; bi, bipolar cell dendrite; sv, synaptic vesicles; sr, synaptic ribbon; N, number of mice; n, number of electron microscope images of rod-photoreceptor ribbon synapses analyzed to count the synaptic vesicles; ***, 0.001; n.s., not significant. Scale bars: 200 nm.",yes
PMC4431046,Figure_8,oa_package/ec/df/PMC4431046.tar.gz,"[', corpectomies)Multilevel laminectomy with medial facetectomy/ foraminotomy.']","Figure 8 Multilevel laminectomy with medial facetectomy/ foraminotomy. CENTRAL IMAGE: This illustrates the posterior view of a C3-C7 laminectomy accompanied by medial facetectomy/ foraminotomy at the C23, C34, C45, C56, C67 C7T1 levels. (a) Illustration of filed-down Kerrison Rongeur utilized to perform medial facetectomy/foraminotomy and resect hypertrophied/ ossified yellow ligament to decompress the nerve roots in the foramina. (b) Axial illustration of the ventral/foraminal osteophytes, which often require resection with a down-biting curette.(c) Illustration of the down-biting curette employed to remove foraminal disc/spur/osteophytes. (A) Illustration of nerve root exiting into the foramen following performance of a medial facetectomy/foraminotomy. (B) In order to resect foraminal disc or spur, utilizing a micro nerve hook allows for mobilization of the foraminally exiting nerve root. (C)The down-biting curette is then introduced ventral to the root and lateral aspect of the thecal sac to remove disc/spur",yes
PMC9501431,Figure_1,oa_package/67/e7/PMC9501431.tar.gz,"['Conceptualized figures of the diverse rodent models of PSD are displayed in , and its comparative clinical PSD subtypes were also postulated in the associated clinical conditions (Table 1).', 'AcknowledgmentsWe would like to thank Seo-U Kim and Ye-Hun Hwang at Medizin Atelier for their contribution to .', '000000000000078331789707Diverse rodent animal models of post-stroke dementia.']","Figure 1 Diverse rodent animal models of post-stroke dementia. (Middle cerebral artery occlusion, MCAO; bilateral common carotid artery occlusion, BCCAo; Alzheimers disease, AD).",yes
PMC4256706,Figure_2,oa_package/9f/e0/PMC4256706.tar.gz,[],Figure2: ( ) A CT scan revealing a large hepatic mass invading the abdominal wall.,yes
PMC7379307,Figure_6,oa_package/15/bf/PMC7379307.tar.gz,['HSPB6 expression in ALS spinal cord.'],"Figure 6 HSPB6 expression in ALS spinal cord. HSPB6 expression in ( , , ) ventral horns and ( , , ) lateral tracts of controls and ALS patients (subgroups: SDD and MDD) with ( ) an insert of a vimentin+ (pink) and HSPB6+ (brown) astrocyte. Grey matter is delineated with a dotted line. Quantification of HSPB6+ pixels in ( ) ventral horns and ( ) lateral columns of controls and ALS patients (subgroups: SDD and MDD). Data points represent the mean value for each patient. Data are shown as meanSEM. Significance was analysed between ALS patients ( =12) and controls ( =10) with Student's test or MannWhitney test. ALS patients with SDD ( =7) and MDD ( =5) were compared to controls ( =10) using and Tukey's posttest or KruskalWallis test and Dunn's multiple comparisons test. Scale bar in all pictures=50m. Inserts are digitally enlarged. SDD, short disease duration; MDD, moderate disease duration; ALS, amyotrophic lateral sclerosis; HSPB, heat shock protein B.",yes
PMC9713829,Figure_1,oa_package/d0/07/PMC9713829.tar.gz,"['The lesion was showing high signal intensity on T2-weighted imaging but no contrast enhancement ().', '1186/1477-7800-2-181615015611ZouLZhangXXiangHMalignant mixed epithelial and stromal tumor of the kidney: The second male case and review of literatureInt J Clin Exp Pathol72658266320142496698212MinodaRTakagiTTodaNItagakiHKondoTIshidaHNagashimaYTanabeKBilateral and multiple mixed epithelial and stromal tumors of the kidney: A case reportMol Clin Oncol710051007201729285364.']",Figure 1. Lesion as observed using magnetic resonance imaging. (A) Frontal plane and (B) sagittal plane. The images indicate an incidentally revealed pathologic lesion of the left kidney (hollow arrow) containing a small solid component adjacent to its wall (filled arrow). The lesion is well-demarcated and shows high signal intensity on T2-weighted imaging.,yes
PMC3298780,Figure_4,oa_package/a4/a2/PMC3298780.tar.gz,['Perioperative image of the resected ischemic jejunal segment.'],Figure 4 .,yes
PMC3312247,Figure_1,oa_package/99/40/PMC3312247.tar.gz,"['The second and third segments were dislocated together; the second one anteriorly and the third posteriorly ().', '1 lk RAvc AOnatSSayhanMB z elikCTraumatic sternal fracture: Three years experience of thoracic surgery departmentAnatolian Journal of Clinical Investigation20082116182WatanabeSNakamuraTShimizuYHitomiSIkadaYTraumatic sternal segment dislocation in a childChest198996368468625488163WooCCTraumatic manubriosternal joint subluxations in two basketball playersJournal of Manipulative and Physiological Therapeutics198811543343732359314WatanabeHChigiraMShimizuTAoyamaMCompletely remodeled dislocated sternal segment in a childArchives of Orthopaedic and Trauma Surgery19931122888984574185SuzukiHKanekoFGomibuchiFEgawaASternal segment dislocation; a case reportSeikeigeka198940727729Lateral chest X-ray.']",Figure 1 Lateral chest X-ray. Dislocated sternal segments.,yes
PMC11066433,Figure_4,oa_package/22/3a/PMC11066433.tar.gz,"['4"">Bifurcation analysis of dermal senescenceA nonlinear ordinary differential equation (ODE) model of the core network consisting of TGF- 1, THBS1, and FMOD was constructed to qualitatively elucidate the behavior of the system ( 4A, see details in the STAR Methods section).', 'We examined which parameter is more influential for controlling the steady state, and found that both parameters can induce a bistable switch for TGF- 1 ( 4B).', 'gif"">K2 ( 4B): changes in K2 (right panel), which correspond to the cut surfaces shown in 4B.', 'To better understand the roles of endogenous TGF- 1, THBS1, and FMOD, we extended the aforementioned model as an endogenous core model to incorporate the effects of PDL and endogenous TGF- 1 production for simulating the system behavior in an endogenous condition ( 4D).', 'Firstly, we performed time course washout experiments of TGF- 1 treatment to confirm the irreversibility of the regulatory system, which was suggested by the existence of stable and unstable states ( 4E).', 'Thirdly, to further validate the presence of a binary high-/low-TGF- 1 switch, we examined the bistability of THBS1 expression using immunofluorescence imaging ( 4F).', 'Quantification of integral intensity of each image revealed that THBS1 expression was significantly upregulated by TGF- 1 in a concentration-dependent manner ( 4F), which was consistent with the results obtained through western blot ( 3A).', 'A bimodal to unimodal shift in THBS1 expression by TGF- 1 was suggested based on the simulations of bifurcation analysis ( 4C).', 'gif"">K2 ( 4D).', 'Moreover, for simulating the immunostaining experiment ( 4F), we extended the model (4) (6) to a stochastic model by computing probability distributions of .']","Fig. 4. 39-year-old male with tophaceous gout presenting as a palpable peri-patellar mass. Sagittal proton-density-weighted fat-suppressed MR image shows expansile intermediate-signal soft tissue (arrowheads) within the patellar tendon, extending superficial to the anterior patella. Axial proton-density-weighted fat-suppressed image shows the mass in the patellar tendon (arrow), as well as additional heterogeneously intermediate-signal nodules within the popliteus tendon (curved arrow) and along the medial femoral condyle (arrowhead), in a distribution characteristic of tophaceous gout",yes
PMC11077617,Figure_3,oa_package/f3/77/PMC11077617.tar.gz,"['T2 weighted MRI demonstrating mild bilateral neural foraminal narrowing at L5-S1, shown with arrows.']","Figure 3 T2 weighted MRI demonstrating mild bilateral neural foraminal narrowing at L5-S1, shown with arrows.",yes
PMC4122400,Figure_1,oa_package/78/46/PMC4122400.tar.gz,"['ResultsAngiotensin II contributes to increased vascular TLR4 gene expression in SHRsTLR4 mRNA expression was greater in aortic segments from SHRs when compared with those from Wistar rats; this greater expression was reduced after treatment of SHRs with the AT1 receptor antagonist losartan (\nA\n), which suggests that Ang II contributes to this increased expression.', 'Similarly, TLR4 mRNA levels were greater in VSMCs from SHRs than from Wistar rats (\nB\n).', 'g001Ang II contributes to the increased TLR4 mRNA levels observed in SHRs.', 'g001""/>Immunofluorescence experiments confirm the greater expression of TLR4 in aorta from SHR when compared with Wistar rats; this receptor was localized in the three layers of the vascular wall (\nC\n).']",10.1371/journal.pone.0104020.g001,yes
PMC4161001,Figure_2,oa_package/d1/88/PMC4161001.tar.gz,['Example of the fornix sign.'],"Figure 2 . The axial (left), coronal (middle), and sagittal (right) slices of the color-scaled FA map are shown with the magnified view of the fornix (yellow rectangle). A cognitively normal 81-year-old woman without the fornix sign. The core part of the fornix appears yellow to red (FA 0.50.8). A cognitively normal 80-year-old man with the fornix sign. The fornix appears green (FA < 0.5). He converted to amnestic MCI (aMCI) 1 year after the scan. An 80-year-old man with aMCI without the fornix sign. He was stable during 3 years observation period after the scan. A 79-year-old man with aMCI with the fornix sign. He converted to AD 1 year after the scan. A 74-year-old man with aMCI without the fornix sign. He converted to dementia with Lewy bodies 3 years after the scan. An 81-year-old woman with AD with the fornix sign. FA, fractional anisotropy.",yes
PMC6235148,Figure_3,oa_package/74/6a/PMC6235148.tar.gz,"[': Examples of visible lesions of the gills and eyes of fish.', 'com/files/ftp_upload/57946/57946fig3large.']",,yes
PMC10470087,Figure_4,oa_package/c1/34/PMC10470087.tar.gz,"['IFN expressing CD4+ and CD8+ T cells accumulated in the lung of Ad5-N-vaccinated female BALB/c mice upon re-encountering the antigen.', '4DiscussionAlong with other viral protein(s), SARS-CoV-2 N-based vaccines confer protection in various animal models against infection by SARS-CoV-2 ancestral virus and variants of concern (Dangi et al.']","Fig. 4 IFNexpressing CD4 and CD8 T cells accumulated in the lung of Ad5-N-vaccinated female BALB/c mice upon re-encountering the antigen. (A) Representative gating strategy for flow cytometry analysis of CD4 and CD8 T cells in intraparenchymal lung lymphoid cells isolated from PBS-, Ad5-vec-, and Ad5-N-inoculated mice 5 days after 10-fold Ad5-N challenge. (B) Quantification for flow cytometry analysis of CD4 and CD8 T cells. Data are shown as mean SD. ns, not significant; * < 0.05 (n=7 mice per group, one-way ANOVA). (C) Representative gating strategy for flow cytometry analysis of CD11a and IFN- expression in CD4 and CD8 T cells. (D) Quantification for flow cytometry analysis of CD11a and IFN- expression in CD4 and CD8 T cells. Data are shown as mean SD. ns, not significant; *** < 0.001 (n=7 mice per group, one-way ANOVA). (E and F) Representative images of immunohistochemistry analysis of the accumulation of CD8 cells in 10-fold Ad5-N-challenged (E) or SARS-CoV-2-infected (F) lung sections (Scale bar, 50 m).",yes
PMC11334941,Figure_8,oa_package/ef/c5/PMC11334941.tar.gz,['Otoscope and OCT image acquired from TM retraction patient.'],Fig. 8 Otoscope and OCT image acquired from TM retraction patient. (a)A snapshot of the otoscopic video camera. Dashed colored lines indicate the positions where the B-scans are shown in panels (c)and (d). (b) image generated by SVP from an OCT volume. Dashed colored lines indicate the positions where the linear B-scans are shown in panels (c)and (d). (c)B-scan extracted along the green-colored dashed line from the OCT volume and overlaid with the gray-shaded OCT image (labeled 3) acquired from a normal condition. (d)B-scan extracted along the red-colored dashed line from the OCT volume. White arrows with circled numbers 1 and 2 denote that the TM touched the incus and was close to the cochlear promontory.,yes
PMC8615430,Figure_2,oa_package/6b/3d/PMC8615430.tar.gz,"['Effect of DAS/HP- -CD 10% on -DG Protein Levels in mdx DIA and GC MusclesThe ability of the novel formulation to restore -DG expression and decrease its phosphorylated form in dystrophic skeletal muscles was assessed by Western blot ().', 'In detail, we measured -DG levels (expressed as ratio on reference standard, vinculin) and the p- -DG/ -DG ratio in DIA (A C) and GC (D F) muscles.', 'Untreated mdx mice showed a significant reduction of -DG levels in both muscles (B,E), paralleled by a significant increase in p- -DG/ -DG ratio (C,F) with respect to WT mice.', 'The 12-week treatment with dasatinib induced a clear trend toward increment of -DG levels in either DIA (B) or GC (E) muscle, with the 20 mg/kg dose being more efficient in restoring -DG protein expression, as shown by the recovery scores (10 mg/kg: 46% for DIA, 25% for GC; 20 mg/kg: 76% for DIA, 67% for GC).', 'untreated ones (C).', 'A similar trend was observed in GC muscle, with a milder reduction in p- -DG/ -DG ratio at both doses (recovery score: 66% and 47%, respectively) (F).', 'In (A,D) are shown representative Western blots for total -dystroglycan ( -DG), phosphorylated (p-) -DG, and reference standard vinculin (VINC) proteins, carried out in diaphragm (DIA) and gastrocnemius (GC) muscles, respectively.']","Figure 2 In ( , ) are shown representative Western blots for total -dystroglycan (-DG), phosphorylated (p-) -DG, and reference standard vinculin (VINC) proteins, carried out in diaphragm (DIA) and gastrocnemius (GC) muscles, respectively. Total -DG levels on VINC, and p--DG/-DG ratio, are represented in ( , ) for DIA, and in ( , ) for GC muscle. Values are expressed as mean SEM obtained from WT mice (n = 46), and mice treated with vehicle (VEH; n = 57), or DAS/HP--CD 10% at the dose of 10 (n = 79) or 20 (n = 68) mg/kg. For DIA muscle, a statistically significant difference among groups was found by one-way ANOVA for -DG/vinculin ( ); F = 3.6, < 0.03 and p--DG/-DG ratio ( ); F = 5.5, < 0.007. Bonferroni post hoc test for individual differences between groups is as follows: * vs. WT ( < 0.03), vs. + vehicle ( < 0.006). For GC muscle, a statistically significant difference among groups was found by one-way ANOVA for -DG/vinculin ( ); F = 3.8, < 0.02 and --DG/-DG ratio ( ); F = 3.2, < 0.05. Bonferroni post hoc test for individual differences between groups is as follows: * vs. WT ( < 0.05); N.S. vs. + vehicle ( > 0.05).",yes
PMC11320541,Figure_7,oa_package/83/62/PMC11320541.tar.gz,"['Benign tumors in the left tibia on radiographs were found by one of the two junior radiologists but missed by the other junior radiologist and were not detected by the DL model.', 'On the other hand, several relatively small or inconspicuous bone changes in bone tumors were not detected by the DL model despite the overall high sensitivity of the DL model ().']","Figure 7 Benign tumors in the left tibia on radiographs were found by one of the two junior radiologists but missed by the other junior radiologist and were not detected by the DL model. (A) A bone tumor in the diagnostic reference standard box on the radiographs. (B) The tumor evaluated in the predicted box by an observer. L, left; DL, deep learning.",yes
PMC6137398,Figure_4,oa_package/b7/9b/PMC6137398.tar.gz,"['Based on the age of the patient and the working hypotheses, the surgical team decided to proceed to a complete surgical excision of the tumor.']","Fig. 4 Axial enhanced CT images during arterial (a) and venous (b) phases. After contrast administration, the mass showed a heterogeneous, predominantly hypovascular enhancement pattern, with an enhancing nodular capsule (a). The venous and late phases showed a progressive homogenous enhancement pattern (b). The mass was in close proximity with the greater curvature of the stomach, with no signs of invasion.",yes
PMC5114312,Figure_3,oa_package/0e/86/PMC5114312.tar.gz,['Survival rates of larvae with a systemic infection after injection of different dosages of W.'],"Figure 3 , while injection into the swim bladder has no impact on larval survival .",yes
PMC8630525,Figure_9,oa_package/1a/b3/PMC8630525.tar.gz,"['05; A-D).', '.']","Figure 9. Successful transfection of RhoA overexpression plasmid in AML cells. Western blot analysis of (A) KG-1 and (B) Kasumi-1 cells following RhoA overexpression. RhoA and ROCK1 protein expression levels in (C) KG-1 and (D) Kasumi-1 cells following RhoA overexpression. *P<0.05, **P<0.01. RhoA, ras homolog family member A; AML, acute myeloid leukemia; ROCK1, Rho associated coiled-coil containing protein kinase 1; NC, negative control; KIF2A, kinesin family member 2A; si, small interfering.",yes
PMC6109099,Figure_6,oa_package/57/4c/PMC6109099.tar.gz,"[' 6a, b, Supplementary 8A and 8B).', ' 6a, c).', ' 6c, d) as canonical Notch signaling is a major regulator of epidermal differentiation6, which may trigger aberrant differentiation in JunB cKO skin.', ' 6e, Supplementary 9A and Supplementary 12).', ' 6e and Supplementary 9A).', 'JunB deficiency significantly altered the global transcriptome.', 'A schematic representation of JunB/AP1 binding sites (Red bar) in the promoter region of either Notch1 or Notch4 gene is presented above the graphsNext, to discover the potential role for JunB/AP1 in the regulation of differentially expressed genes and accessibility to gene-regulatory chromatin regions, an assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) analysis was employed.', ' 6f, Supplementary 10A and 10B), which most likely influence global gene transcription.', ' 6g).', ' 6h), and Notch 4 gene (', ' 6i) were confirmed, indicating direct regulation of Notch signaling by JunB in the epidermis.']","Fig. 6 JunB deficiency significantly altered the global transcriptome. , Heatmap depicting transcriptome profiling of samples ( =3) from wild type and JunB cKO skin harvested after wounding or hair plucking. The color reflects the log2 scale of relative expression as in the case of Figure ( , , and ). Pathway enrichment analyses depicting highly upregulated pathways in JunB cKO mice skin compared to wild type after wounding or hair plucking. Dashed line represents value of 0.05. <0.05 for the pathway enrichment was considered significant. Heatmap depicting genes involved in Notch signaling from wild type and JunB cKO skin collected post hair plucking. Western blot analyses of key activated Notch pathway proteins and AP-1 members 4 days post hair plucking in JunB cKO and wild type skin. Distribution of ATAC-seq signals across the gene body of the whole mouse genome and high enrichment of the AP-1 motif both in primary epidermal progenitor cells isolated from JunB cKO and wild type skin post hair plucking. ChIP assay from wild type epidermal progenitor cells isolated after hair depilation displays distinct JunB binding sites in and promoter regions. The fold enrichment is calculated by comparing the Ct values of each primer sets (JunB/AP1 binding site) with a JunB/AP1 negative region (3 UTR in case of and 5 UTR in case of ). Each of the Ct values of either JunB/AP1 binding sites or negative region was subtracted from respective IgG Ct values ( =3), * <0.05, *** <0.001, -test. The position of motif (JunB binding site) is mentioned in the X axis. A schematic representation of JunB/AP1 binding sites (Red bar) in the promoter region of either or gene is presented above the graphs",yes
PMC9197745,Figure_1,oa_package/9c/7d/PMC9197745.tar.gz,[],Image 1 A point-of-care ultrasound image obtained with a linear transducer in the transverse plane illustrates a heterogeneous collection superior and to the right of the urinary bladder surrounded by hyperechoic inflammatory changes. (U = urachal cyst; B = bladder),yes
PMC5601994,Figure_13,oa_package/af/78/PMC5601994.tar.gz,[],10.7554/eLife.29236.017,yes
PMC9143363,Figure_1,oa_package/cb/4e/PMC9143363.tar.gz,"['Hence, retreatment was not required ().', 'A717434117016The images show a wide neck aneurysm of the apex of the basilar artery treated using WEB 3 2 mm.']","Figure 1 The images show a wide neck aneurysm of the apex of the basilar artery treated using WEB 3 2 mm. ( ) not-substracted DSA at 3-years, ( ) substracted DSA at 3-years. An aneurysm adeguate occlusion was detected and confirmed by 3D protocol.",yes
PMC4275855,Figure_2,oa_package/c2/fa/PMC4275855.tar.gz,"['It was located between the urinary bladder and adjacent rectum causing an indentation on the bladder wall (a and b).', '(a) A slightly calcified 14 mm lesion between the bladder and rectosigmoid junction.']",Fig. 2 (a) A slightly calcified 14mm lesion between the bladder and rectosigmoid junction. (b) The cystic lesion without penetration of contrast medium from the bladder (cystographic lateral view).,yes
PMC5601392,Figure_2,oa_package/c4/eb/PMC5601392.tar.gz,"['The echocardiography results are shown in .', '.']","Figure 2. Transthoracal echocardiography measurements at initial admission in 2014. Left ventricle (LV) showed concentric hypertrophy (interventricular septal thickness 1.2 cm) in parasternal long-axis view ( ), four chamber view ( ), m-mode ( ) and left-to-right flow across the secundum ASD in the apical four chamber view ( ).",yes
PMC9515104,Figure_2,oa_package/bc/77/PMC9515104.tar.gz,"[' 2 visualizes four exemplary patches with a high inter-rater agreement for the first two examples and a low inter-rater agreement for the second two.', 'Inter-rater variability for four exemplary test patches.', 'Table 3Class-wise conformity index computed for all unique pairs of annotators.', ' 2.', ' 2.']",Fig. 2 Inter-rater variability for four exemplary test patches. ( ) Original patch. ( ) Annotations of pathologists. The first two rows show examples with a high inter-rater concordance and the second two rows examples with a low inter-rater concordance. The third example shows different definitions for dermis (blue) and subcutis (red) whilst the fourth example shows a high variation for inflammation & necrosis (pink).,yes
PMC4944637,Figure_2,oa_package/e0/02/PMC4944637.tar.gz,"['Cystoscopy revealed a bladder displacement to the right with difficulty in evaluating the right ureteral orifice [].', 'Cystoscopy with bilateral retrogrades demonstrates a shifted ureteral orifice and bladder to the right pelvic areaThe patient underwent abdominal and pelvic exploration with surgical excision of the pelvic sarcoma with bladder-sparing.']",Figure 2 Cystoscopy with bilateral retrogrades demonstrates a shifted ureteral orifice and bladder to the right pelvic area,yes
PMC9933174,Figure_6,oa_package/e1/83/PMC9933174.tar.gz,"['Furthermore, intestinal IPMN displayed highly differentially methylated genes, such as FOXP2, DCLK1 (doublecortin-like kinase 1) and BICC2 (bicaudal C homolog 2) (online supplemental figure 6), which can be ascribed to progenitor markers, possibly suggesting the presence of a still unidentified progenitor-like cell population in the pancreatic ductal system.', 'Based on these results and on recently published data, a model of development of gastric and intestinal pancreatic precursors can be proposed (figure 6).', 'As shown in figure 6, the occurrence of mixed phenotypes, and also the evolution from gastric to intestinal IPMN, as suggested by some,16 could be explained by this model.', 'Model of development of pancreatic cancer precursors.']","Figure 6 Model of development of pancreatic cancer precursors. mutations induce a gastric phenotype characteristic of mostly peripherally located lesions, such as PanIN and gastric IPMN, which are additionally Notch-dependent. Recurrent deletions occur only in gastric IPMN. These share a very similar mucin profile with PanIN, but they can be distinguished to some extent by different MUCL3 expression, with lack of expression arguing against gastric IPMN. Further stimuli, such as exogenous factors related to a different microenvironment and possibly acting on a minor MUC5B-positive ductal cell population, induce an intestinal phenotype, driven by and/or mutations, with differential regulation of the mucin type O-glycan biosynthesis, expression of MUC2, CDX2 and TFF3 and recurrent amplifications. Mixed phenotypes and/or a transition from a gastric to an intestinal phenotype may also occur. CNV, copy number variation; IPMN, intraductal papillary mucinous neoplasms; MUC1, mucin 1; MUC2, mucin 2; MUC5AC, mucin 5; PanIN, pancreatic intraepithelial neoplasias; TFF3, trefoil factor 3.",yes
PMC6899892,Figure_7,oa_package/8f/e2/PMC6899892.tar.gz,['A model of how CCN5 may intervene fibrosis via transforming growth factor (TGF ) pathway.'],"Figure 7 A model of how CCN5 may intervene fibrosis via transforming growth factor (TGF) pathway. Exogeneous TGF1 induces fibroblast to myofibroblast transdifferentiation. Knock out (KO) of transforming growth factor receptor 1 (TGFBR1), reduces the effect of TGF1. Treatment of CCN5 decreases the transdifferentiation of fibroblast to myofibroblast by exogenous TGF1. [Color figure can be viewed at wileyonlinelibrary.com]",yes
PMC8535251,Figure_1,oa_package/49/a5/PMC8535251.tar.gz,"[' and Table A2 present the prevalence of Fukuda prevalence in the whole sample and in females and males.', '1038/s41598-020-66784-232546704Spider chart on the prevalence of Fukuda symptoms in the whole sample (A) and in females and males (B).']",Figure 1 Spider chart on the prevalence of Fukuda symptoms in the whole sample ( ) and in females and males ( ).,yes
PMC6700566,Figure_3,oa_package/f0/09/PMC6700566.tar.gz,"['First, PBP was secured through maximal inflation of the balloon of the BGC (figure 3A).', 'The balloon of the GW was inflated to 6 mm to establish the DBP (figure 3B).', 'Schematic illustrations and fluoroscopic images explaining our method of endovascular thrombectomy under a direct aspiration first-pass technique double balloon protection (ADAPT-DBP).', 'Second, the ACE was moved slowly to the carotid bifurcation under direct aspiration via the ACE which was directly connected with the Large Lumen Aspiration Tubing, a MAX Canister and a MAX Pump (figure 3C).', 'Finally, we repeated the manual aspiration several times via a 6Fr Advance Aspiration Catheter (Medtronic) (figure 3D), which we had navigated through the GW.']","Figure 3 Schematic illustrations and fluoroscopic images explaining our method of endovascular thrombectomy under adirect aspiration first-pass technique double balloon protection (ADAPT-DBP). Through a 300cm Carotid GUARDWIRE PS (GW), a 5MAX ACE068 Reperfusion Catheter (ACE) was inserted into the lumen of the balloon guiding catheter (BGC) and guided to the proximal right carotid bifurcation. (A) The balloon of the BGC was maximally inflated to protect the right common carotid artery. The GW was carefully advanced across the free-floating thrombus (FFT) into the right internal carotid artery (ICA) and positioned at the right cervical ICA just near the petrous. (B) The balloon of the GW was inflated and DBP was established. (C) The ACE was carefully advanced to the FFT and the thrombus was aspirated by means of ADAPT. (D) Through the GW, the ACE and an aspiration catheter were exchanged, and manual aspiration was performed with the aspiration catheter. Finally, the BGC and the GW were deflated. The schemas in the upper panel were drawn by AY.",yes
PMC6150703,Figure_4,oa_package/03/b0/PMC6150703.tar.gz,"['\n\nMicroscopic aspects of rat molar root presented in , at greater magnification, in axial section, after 4 days under moderate orthodontic forces.']","Figure 4 Microscopic aspects of rat molar root presented in , at greater magnification, in axial section, after 4 days under moderate orthodontic forces. The image reveals the activity of clasts and other cells, thanks to the maintenance of periodontal structures, without hyalinization (HE, 40X magnification).",yes
PMC9310243,Figure_2,oa_package/77/43/PMC9310243.tar.gz,"['In case 1, tumour cells diffusely infiltrated bilateral thyroid tissues, mostly in dilated lymphatic fissures (a).', 'Cancer cells were arranged around psammoma bodies or loose connective tissue (b), presenting with a papillary structure and some of them had a hobnail-like appearance.', 'A large number of psammoma bodies, infiltration of lymphocytes, formation of lymphatic follicles and extensive squamous metaplasia were observed (c).', 'In more areas, the nuclear chromatin pattern of the carcinoma cells was slightly coarser than that of conventional papillary carcinoma and the nuclei were large and round, while the typical papillary nuclei (grooved nuclei and cytoplasmic inclusions) were not obvious (d).', '.', '1177_03000605221099465-fig2"" position=""float""/>In case 2, a large number of scattered calcifications were observed in the background of nodular goitre (a).']","Figure 2. Representative photomicrographs of the tumour specimen from case 1: (a) tumour cells diffusely infiltrated the bilateral thyroid tissues, mostly in dilated lymphatic fissures (haematoxylin and eosin [H&E]; 40); (b) tumour cells were arranged around psammoma bodies, presenting with a papillary structure (H&E; 100); (c) a large number of psammoma bodies, infiltration of lymphocytes, formation of lymphatic follicles and extensive squamous metaplasia were observed (H&E; 100) and (d) the nuclear chromatin pattern of the carcinoma cells was slightly coarser than that of conventional papillary carcinoma and the nuclei were large and round, while the typical papillary nuclei (grooved nuclei and cytoplasmic inclusions) were not obvious (H&E; 200). The colour version of this figure is available at:",yes
PMC7592533,Figure_2,oa_package/ee/ae/PMC7592533.tar.gz,"[' 2a).', ' 2b).', 'Histopathological classification of tumour and tumour stroma zones identifies initial acinar adenocarcinoma and progression to small cell carcinoma.', 'Scale bar indicates 100 mBiomarker staining reveals androgen-intact tumor with NEPC-like marker expression.']","Fig. 2 Histopathological classification of tumour and tumour stroma zones identifies initial acinar adenocarcinoma and progression to small cell carcinoma. (A) Overview of H&E stained sections used for histopathological and molecular analysis. Tissue zones comprising (i) tumour, (ii) tumour stroma, and (iii) mixed are indicated. Scale bar indicates 2mm. High magnification H&E stains of tumour stroma (one zone) and tumour (three zones) at each tissue collection timepoint. Scale bar indicates 100m",yes
PMC9405762,Figure_1,oa_package/50/8b/PMC9405762.tar.gz,"['In agreement with previous results [9], in which we used biotinylated CTXb and streptavidin Alexa488 fluorophore, CLSM imaging and analysis revealed a ~70% increase in the mean fluorescence intensity in the fibroblasts from the juvenile patient affected by GM1 gangliosidosis with respect to the fibroblasts from an unaffected donor (A).', 'To investigate whether flow cytometry could be used to rapidly detect changes in the levels of GM1 not only in lymphocytes [9] but also in adherent cells, we detached, fixed, permeabilized, and labeled the patient and control fibroblasts with CTXb-FITC (B).', '5-fold increase was found in the fibroblasts from an infantile patient (Pt3), further corroborating the link between the severity of the disease and the accumulation of GM1 in the lymphocytes [9] (C).', '1515/bmc-2013-003925372744GM1 gangliosidosis.']","Figure 1 GM1 gangliosidosis. Thawed primary cultures of fibroblasts isolated from control and GM1 gangliosidosis patients were cultured, fixed, permeabilized, labeled with CTXb-FITC, and imaged with CLSM ( ) or analyzed with flow cytometry ( , ). ( ) The juvenile patient (Pt1) shows higher values of CTXb-FITC fluorescence intensity compared to WT, indicating an increase in GM1 content. Scale bar 30 m. >20 cells were analyzed for each condition. Error bar S.D. ( ) Schematic representation of the workflow to analyze the cells of affected and unaffected cells with flow cytometry. The FSC-A vs. SSC-A plot is gated (blue line) to exclude cellular debris, the corresponding CTXb-FITC fluorescence intensity distributions of affected (red line) and unaffected (black line) are analyzed, and median fluorescence values (MFI) are extrapolated. ( ) MFI/MFI values are obtained by dividing the MFI of a distribution by the mean MFI obtained from the controls. MFI/MFI values increase proportionally to the severity of the pathology in juvenile (Pt1 and Pt2) and infantile patients (Pt3). >5000 cells were analyzed for each MFI. Students -test ** 0.01, *** 0.001.",yes
PMC4533765,Figure_7,oa_package/3f/6a/PMC4533765.tar.gz,"[' 7a, c) than in the ADSC group (', ' 7b, d) (P 0.', ' 7e, black arrows) was much greater when compared with ADSC-alone treatment (', ' 7f, black arrows) (P 0.', 'Histology staining of the ischemic muscles after cell transplantation.', 'ADSC adipose-derived stem cell, P-ADSC periostin-transfected ADSCCytokines and growth factors in P-ADSCsTo detect paracrine factors, we collected the supernatant of transfected or untransfected ADSCs at 48 hours for ELISA.']","Fig. 7 Histology staining of the ischemic muscles after cell transplantation. Representative fluorescent staining for -actin ( ), ADSCs ( ), and nuclei with DAPI ( ) in the P-ADSC group and in the ADSC group . Representative immunohistochemistry staining for endothelial cell markers CD31 ( ) in the P-ADSC group and in the ADSC group . Representative HE staining in the P-ADSC group and in the ADSC group ( , blood vessels). Scale bar = 100 m. adipose-derived stem cell, periostin-transfected ADSC",yes
PMC10706240,Figure_3,oa_package/59/33/PMC10706240.tar.gz,"[' displays a selection of melanoma and nevus images.', 'Different skin lesion examples in the MED-NODE dataset 10: (a) melanoma; (b) nevus.']",Figure 3 Different skin lesion examples in the MED-NODE dataset 10: ( ) melanoma; ( ) nevus.,yes
PMC11600427,Figure_1,oa_package/1f/6b/PMC11600427.tar.gz,"['To investigate BBB integrity in tau-associated pathology, we utilized WEPCAST MRI and longitudinally assessed BBB permeability in PS19 mice at 3, 6, and 9 months (A).', 'However, at 9 months old, PS19 mice displayed significantly increased water extraction fraction and BBB permeability to water compared to WT mice, while global cerebral blood flow (CBF) remained similar (B E).', 'Notably, D + Q treatment preserved BBB integrity in 9-month-old PS19 mice, restoring it to the normal range observed in wild-type littermate controls (B D), without impacting on CBF (', 'Remarkably, PS19 mice in the vehicle-treated group exhibited significant weight loss at 9 months old, while D + Q treatment rescued their body weight (F).', '18215617\n.', '']","Fig. 1. DQ treatment maintains blood-brain barrier (BBB) integrity in 9 months old PS19 mice. (A) Timeline of the experiment design and outcome measurements. (B) Representative images of WEPCAST MRI in indicated groups. Arrows indicate the Great vein of Galen (GVG) where the WEPCAST MRI detects the amount of magnetically labeled water. Warm colour indicates higher amounts of magnetically labeled water. Note that PS19 brain has reduced magnetically labeled water in the GVG. (C and D) Water extraction fraction and permeability surface area product (PS) were calculated according to the formulas described in the method section. Higher water extraction fraction and PS values indicate increased BBB permeability to water. = 69 mice/group * < 0.05 by one-way ANOVA with Bonferroni correction for multiple comparisons. (E) Cerebral blood flow (CBF) evaluated by MRI in the same mice was used for BBB measurement. = 79 mice/group; ns, no statistical significance by one-way with Bonferroni correction for multiple comparisons. (F) Body weights of the mice underwent BBB and CBF measures at indicated ages. = 915 mice/group; *p < 0.05 by one-way ANOVA with Bonferroni correction for multiple comparisons. In all graphs, each individual data points are indicated; Red dots represent female mice and blue dots represent male mice. All data are shown as individual data points, Mean and SD. Wild type treated with vehicle (WT + Veh), PS19 mice treated with vehicle (Tau +Veh) or DQ (Tau+DQ).",yes
PMC10731592,Figure_1,oa_package/43/9c/PMC10731592.tar.gz,"['On admission, flaccid bullae were observed on the intertriginous skin folds, bilateral thighs, upper arms, and posterior neck with a positive Nikolsky sign in many areas ().', 'Case 1, Toxic epidermal necrolysis like bullous drug rash.']","Fig 1 Case 1, Toxic epidermal necrolysislike bullous drug rash. The patient had flaccid bullae on a background of erythema on the trunk, pelvis, and bilateral midthighs with notable skin sloughing on her lateral left thigh. There was significant separation of overlying epithelium in the intertriginous areas of the pelvis and axilla.",yes
PMC8587380,Figure_1,oa_package/a2/4b/PMC8587380.tar.gz,"['InvestigationsThe CT scan on presentation confirmed the previously described bone fractures, CCF and multiple old haemorrhagic contusions in the right temporal, frontal and parietal zones (figure 1).', 'Three-dimensional reconstruction of the intracranial vessels carotid cavernous fistula is visualised in the right cavernous sinus.']",Figure 1 Three-dimensional reconstruction of the intracranial vesselscarotid cavernous fistula is visualised in the right cavernous sinus.,yes
PMC9955517,Figure_4,oa_package/5f/10/PMC9955517.tar.gz,"['These tumors are often associated with intralesional hemorrhage and hemosiderin deposition; because hemosiderin includes paramagnetic Fe+3 atoms, the local magnetic field around areas of hemosiderin deposition become distorted, resulting in blooming artifacts on gradient-echo or susceptibility-weighted sequences ().', 'Representative images of tenosynovial giant-cell tumors of tendon sheath, previously known as PVNS.']","Figure 4 Representative images of tenosynovial giant-cell tumors of tendon sheath, previously known as PVNS. ( ) Sagittal T1-weighted image showing a volar hypointense homogenous mass at the level of the PIP joint. ( ) Coronal T1-weighted image demonstrating a hypointense homogenous mass on the ulnar aspect of the index finger, consistent with TGCT of tendon sheath. ( ) Gradient-echo coronal image of the hand demonstrating a hypointense mass of the index finger with associated distorted artifact signal (yellow arrow), so called blooming artifacts consistent with TGCT of tendon sheath. This characteristic appearance is secondary to intralesional hemorrhage and hemosiderin deposition; because hemosiderin includes paramagnetic Fe+3 atoms, the local magnetic field around areas of hemosiderin deposition become distorted. ( ) Longitudinal ultrasound of the index finger shows the palmar component of the tenosynovial giant-cell tumor as a hypoechoic lobular mass (arrows), volar to the flexor tendon (arrowheads). The diagnosis was proven by ultrasound-guided percutaneous needle biopsy.",yes
PMC6329060,Figure_1,oa_package/bd/d7/PMC6329060.tar.gz,"[' 1a d).', 'a d Segmentation, modeling, and alignment of the 3D pelvic model.', 'd The pelvic model with the anterior pelvic plane perpendicular to the horizontal planeTo calculate PI in 3D models, at least two anatomical references have to be determined, that is, the hip axis and the midpoint of the sacral endplate.']","Fig. 1 Segmentation, modeling, and alignment of the 3D pelvic model. A spherical mask was fitted to isolate the pelvis from CT volume images. Femurs and the lumbar vertebra were manually removed. A virtual 3D pelvic model was automatically reconstructed using a threshold and region-growing algorithm. Both anterior superior iliac spines and pubic tubercles were manually selected to determine the anterior pelvic plane. The pelvic model with the anterior pelvic plane perpendicular to the horizontal plane",yes
PMC3347324,Figure_9,oa_package/8f/78/PMC3347324.tar.gz,"['HCV RNA levels were reduced by 50%, 77% and 80% in FUT1 and by 74%, 77% and 78% in KLHDC7B gene siRNA treated cells, relative to controls, at 24, 48 and 72 hours after HCV infection, respectively (, Panel A).', 'The titer of infectious HCV produced in cell cultures with FUT1 silenced was decreased by 27%, 50% and 50% at 24, 48 and 72 hours after HCV infection, respectively (, Panel B).', 'Silencing of KLHDC7B decreased the titer of infectious HCV produced in cell culture by 72%, 76%, and 66% at the designated times relative to controls (, Panel B).', 'siRNA silencing of FUT1 and KLHDC7B inhibit HCV replication and infectious virus production.']","Figure 9 siRNA silencing of and inhibit HCV replication and infectious virus production. Huh 7.5 cells were transfected with control siRNA (white bars), (gray bars) or (black bars) and infected with JFH-1 (MOI = 0.5) for two hours, supplemented with fresh complete DMEM and continuously cultured to 24, 48, and 72 hours. At each time point, HCV RNA was measured by real-time PCR ( ) and the titer of infectious HCV was measured by serial dilution of culture supernatants ( ). The percent inhibition produced by each siRNA is shown above each bar in the graph.",yes
PMC7042144,Figure_4,oa_package/b6/f5/PMC7042144.tar.gz,['A 53-year-old man with a bicuspid aortic valve underwent elective cardiac surgery involving the David procedure.'],Figure 4 A 53-year-old man with a bicuspid aortic valve underwent elective cardiac surgery involving the David procedure. Four days post-operatively developed non-ST-elevation myocardial infarction. Gated computed tomography angiography suggested distortion of the proximal right coronary artery marked with a blue arrow ( ). This corresponds with the stenosis marked with blue arrows in diagnostic invasive angiography images ( ). There was a good result following implantation of a drug-eluting stent with the stented area marked by the green arrow ( ).,yes
PMC8645490,Figure_1,oa_package/38/f2/PMC8645490.tar.gz,"['3 cm was identified on mammography as seen in [ and 2].', ':A 57-year-old woman who presented with a new breast lump.']",Figure 1: A 57-year-old woman who presented with a new breast lump. Current mediolateral oblique screening mammography images of the right (a) and left (b) breast demonstrating fatty replaced breast parenchyma and a global asymmetry involving the right superior lateral breast.,yes
PMC8481038,Figure_9,oa_package/af/8f/PMC8481038.tar.gz,"['Hematoxylin/eosin staining showed broadly dilated kidney tubules and crystal deposition in the renal tubule lumen of CaOx stone model rats (); the crystallization score was 2.', '008"" position=""float""/>Representative hematoxylin/eosin staining of kidney tubules of the normal control (NC), CaOx stone model M, M + low-dose TFL (LT), M + medium-dose TFL (MT), and M + high-dose TFL (HT) groups (100 magnification).']","Figure 9 Representative hematoxylin/eosin staining of kidney tubules of the normal control (NC), CaOx stone model M, M+low-dose TFL (LT), M+medium-dose TFL (MT), and M+high-dose TFL (HT) groups (100magnification).",yes
PMC3352619,Figure_1,oa_package/6b/a0/PMC3352619.tar.gz,"['5 m/pixel resolution [a].', 'Representative areas of each tissue class of interest were identified in training slides, based upon their histological features, and compiled in a digital montage at 5 magnification [b].', 'Quantifying metastatic mammary cancer in mouse lungs.', 'Lung tissue sections included complete right and left lung sections (dorsal (coronal) plane orientation sections) [a] (provided by Dr.', 'To obtain manual morphometric measurements of tumor burden area, total pulmonary tissue areas and tumor areas were manually outlined using drawing tools in ImageScope software [c].', 'Tissue classes used to create a PRIA algorithm for quantifying lung metastases included multiple examples of normal lung, tumor, and background (glass) from five training slides [b].', 'Following creation of the PRIA algorithm, the 39 lung section images were analyzed by Genie as unknowns (testing set) [d].', 'Pseudo-color mark-up images were provided for all specimens within the testing set as part of the software workflow [d].']","Figure 1 Quantifying metastatic mammary cancer in mouse lungs. (a) Representative metastatic mammary tumors. (b) Features used to train PRIA algorithm [ ]. (c) Manual segmentation performed using the image in (a). Area outlines in green (lung) and yellow (tumor). (d) Image mark-up of (a) following PRIA illustrates lung segmented as blue and metastatic tumor as green. (e) Detail of poorly differentiated tumor (*) and areas of pulmonary atelectasis (arrows). (f) Note, PRIA incorrectly segmented (e). Pulmonary atelectasis (arrows) segmented as false positive for tumor, green. Bar=50 m",yes
PMC8675186,Figure_1,oa_package/34/81/PMC8675186.tar.gz,"['5, 31; , A and B), consistent with previous findings in burn patients (32, 33).', 'Throughout hospitalization, C5a levels fluctuated relative to surgical interventions, which represent additional instances of tissue injury and introduce the risk of bleeding (C).', 'Interestingly, soluble L-selectin, a common marker for leukocyte activation, decreased, although not significantly, during hospitalization (D), consistent with previous findings in trauma patients (36).', '5 days after burn, with the average platelet count nadir occurring at 2 days after burn, along with elevated soluble P-selectin and CD40L in the plasma, indicative of platelet activation (, E G).', 'Previous studies have demonstrated increased circulating thrombomodulin as an indicator of endothelial dysfunction in patients with severe burns (37), but in this heterogeneous cohort, we observed only a minor, nonstatistically significant increase in plasma thrombomodulin on average (H).', 'On a cytokine panel, we observed the greatest difference in plasma between muscle injury alone and burn with muscle injury in IL-6 measurements (Supplemental ; supplemental material available online with this article; 23408937Version 112/08/2021Electronic publicationSIRS and coagulopathy in burn patients.']","Figure 1 SIRS and coagulopathy in burn patients. ( ) Burn patients exhibit markers of inflammation, including ( and ) significantly elevated inflammatory cytokines (IL-6, IL-1) and ( ) markers of complement activation (C5a) upon admission, and ( ) a reduction in leukocyte activation marker soluble L-selectin throughout hospitalization. ( ) Burn patients exhibit hallmarks of coagulopathy including ( ) decreased platelet count with high levels of platelet activation markers, ( ) P-selectin, ( ) CD40L, and ( ) endothelial activation marker thrombomodulin. (* 0.05, ** 0.01, *** 0.001 compared with controls. A Kruskal-Wallis with Dunns post hoc correction for multiple comparisons was performed; 2431 for 0.5, 1, 3, days, and = 715 for 5 or 7 days due to mortality or discharge; 15 for controls. Boxes represent median, while whiskers represent the 5th95th percentile.",yes
PMC2668105,Figure_30,oa_package/a0/a3/PMC2668105.tar.gz,[],"Figure 30 Plexiform lesion of pulmonary hypertension. This classical plexiform lesion is composed of a pulmonary artery profile (upper right of centre) with an adjacent glomeruloid structure (lower left of centre). An early dilatation lesion is also present here (thin-walled, dilated vessels at the edges of the complex). H&E stain, 40 original magnification.",yes
PMC9360087,Figure_1,oa_package/2c/51/PMC9360087.tar.gz,"[' 1).', ""These sagittal illustrations demonstrate the more common anorectal anomalies in males and females (Illustration by Brittany Bennett, MA 2021 Children's Hospital of Philadelphia."", 'All rights reserved)There is a high incidence of associated congenital anomalies with ARM (Table 1).']","Fig. 1 These sagittal illustrations demonstrate the more common anorectal anomalies in males and females (Illustration by Brittany Bennett, MA 2021 Children's Hospital of Philadelphia. All rights reserved)",yes
PMC6247654,Figure_1,oa_package/d8/7e/PMC6247654.tar.gz,"['Both axial and sagittal planes were utilized for this purpose ().', ""13847320Schmorl's node identification in axial (left) and sagittal (right) planes.""]",Figure 1 Schmorl's node identification in axial (left) and sagittal (right) planes.,yes
PMC7605764,Figure_2,oa_package/dd/6c/PMC7605764.tar.gz,['.'],Fig. 2. Best-fit sphere calculated using least-square regression superimposed on the acetabulum. The center of the sphere is shown by a green dot and the acetabulum direction vector by an arrow.,yes
PMC10469149,Figure_9,oa_package/8a/e2/PMC10469149.tar.gz,"['The pathological result confirmed the concurrence of two distinct thyroid diseases in addition to the degenerative changes induced by therapy, a hyperfunctioning goiter with pronounced degenerative changes, imprecisely contoured adenomatous nodules, with areas of hyalinization and interstitial calcification, cystic degeneration, and areas of angiomatosis primarily expressed in the right lobe of the thyroid (A and B).', '(A) Thyroid with slightly contoured nodules.']",Figure 9 (A) Thyroid with slightly contoured nodules. (B) Thyroid with area of hyperfunctioning aspects.,yes
PMC5651626,Figure_1,oa_package/11/26/PMC5651626.tar.gz,['An example of a myelin map of a healthy control subject at several different slices in the dataset described in Section 2.'],,yes
PMC8160561,Figure_10,oa_package/9c/f7/PMC8160561.tar.gz,[],"Fig.10 Tuberculoma: 65-year-old male with no known significant past medical history presented with palpable left upper quadrant mass. Axial plane T2W image showed a semisolid mass with heterogeneous signal intensity (arrows). Axial plane postcontrast T1W image demonstrated heterogeneous enhancement within the lesion (arrows). As findings were found to be concerning for an angiosarcoma, splenectomy was performed. The final pathologic examination confirmed a giant tuberculoma",yes
PMC5024918,Figure_14,oa_package/39/b9/PMC5024918.tar.gz,[],Figure 14 There is a large haematoma indicated by the calipers. It lies deep to the pectoralis minor compressing the nerve cords which cannot be identified on the image on the left. The image on the right was taken post aspiration of the haematoma which shows the collection is smaller and the cords have become more easily seen and are shown outlined by orange dotted lines.,yes
PMC10724790,Figure_7,oa_package/57/7c/PMC10724790.tar.gz,"['4), revision surgery is not recommended even if the location of the anorectum is poor ().', '.']","Figure 7. Schematic explanation of sacral ratio (B:A = 0.74, normally). Sacral ratio calculation on pelvic x-ray of male anorectal malformation case (28:62 = 0.45, sacral dysgenesis).",yes
PMC4097934,Figure_2,oa_package/8b/7f/PMC4097934.tar.gz,"['However, ANA injected animals presented with hearts that appeared enlarged with fat deposits around the atria [a] which was even greater than that observed associated with negative control hearts [b].', 'However, ANA injected animal hearts presented with red stain even within the myocardium (d)The control hearts [c] demonstrated low levels of red in comparison to the ANA positive hearts [d].']","Figure 2 A large amount of fat surrounded the atria of the anti-nuclear antibodies (ANA) positive hearts (a) in comparison to the control hearts [ ] and the negative control hearts (b). Control hearts displayed low levels or red staining (c). However, ANA injected animal hearts presented with red stain even within the myocardium (d)",yes
PMC9581422,Figure_6,oa_package/41/dd/PMC9581422.tar.gz,"['Compared to the vehicle-treated group, the eyes of the lifitegrast-treated group showed a marked decrease in LFA-1 and ICAM-1 protein expression (A).', '1+ cells (B) and CD49a+ NK cells (C) in the Lifitegrast 1 group compared with that of Vehicle 1 group but failed to influence the expression of LFA-1 on CD3 NK1.', 'g006Topical lifitegrast administration inhibited the expression of LFA-1 and ICAM-1 in OT murine model.']",10.1371/journal.pntd.0010848.g006,yes
PMC5342337,Figure_2,oa_package/62/a9/PMC5342337.tar.gz,"['Exogenous FOXC2 expression of mutant recombinant plasmidsA.', 'A similar effect was observed utilizing COS7 cells (Supplementary ).']","Figure 2 Exogenous FOXC2 expression of mutant recombinant plasmids RT-PCR detection of exogenous FOXC2 (741-bp product) and GAPDH (457-bp product) in HeLa transfected cells with p -GFP, p (A3G)-GFP, p (M276DfsX186)-GFP, p (S370T)-GFP, p (Q420X)-GFP, p (L487P)-GFP and p (A492V)-GFP (lanes 3-9); lane 1 refers to DNA molecular weight marker and lane 2 refers to non-transfected HeLa cells. Western blotting analysis of exogenous FOXC2 protein expression in HeLa cells transfected with plasmids reported in (A) and in (B).",yes
PMC5143384,Figure_4,oa_package/6b/31/PMC5143384.tar.gz,"['In contrast to A20LPC-KO mice, which all died between 8 and 25 h post-injection, A20/FADDLPC-DKO mice all support this dose of TNF and only show a modest drop in body temperature similar to wild-type control littermate mice (s 4a and b).', 'In agreement, liver damage, as detected by histology and serum ALT and AST levels, was strongly reduced in A20/FADDLPC-DKO mice, compared with A20LPC-KO mice (s 4c and d).', 'Also IL-6 levels were reduced in A20/FADDLPC-KO mice (e).', 'Finally, procaspase-3 processing could only be detected after TNF challenge in the liver of A20LPC-KO mice, not in control or A20/FADDLPC-DKO mice (f).', 'org/1999/xlink"" xlink:href=""cddis2016154f3""/>A20LPC-KO hepatocytes die from TNF-induced FADD-dependent apoptosis.']","Figure 4 A20 hepatocytes die from TNF-induced FADD-dependent apoptosis. ( ) Body temperature ( ) and survival ( ) of A20/FADD ( =6), A20 mice ( =13) and control (WT; =7) littermates after i.p. injection of 10 g mTNF. *, <0.05; ***, =0.0002 control. ( ) Hematoxylin and eosin (H/E) staining on liver sections from A20 and A20/FADD littermate mice injected with TNF for 5h. Scale bar=100m. ( ) Serum ALT and AST levels of A20/FADD ( =5), A20 mice ( =9) and control (WT; =10) mice 5h after mTNF injection. ( ) Serum IL-6 levels of A20/FADD ( =3), A20 mice ( =7) and control (WT; =5) mice 5h after mTNF injection. ( ) Western blot analysis of A20, FADD, cleaved caspase-3 and actin expression in liver tissue from A20/FADD , A20 mice and control littermate mice (WT) 5h after injection with 10 g mouse TNF. *, <0.05. ALT, alanine aminotransferase; AST, aspartate aminotransferase; FADD, Fas-associated death domain; TNF, tumor necrosis factor; WT, wild type",yes
PMC3177464,Figure_18,oa_package/22/9d/PMC3177464.tar.gz,[],Figure 18 Coronal magnetic resonance T2 fat sat image showing fluid within the medial collateral ligament bursa.,yes
PMC9952238,Figure_2,oa_package/96/98/PMC9952238.tar.gz,"['Public Annotated Cine DatasetsWhile public datasets of CMR images exist, the overwhelming majority are dynamic images acquired with a bSSFP sequence, also referred to as cine images (, top).', ').', 'Example of SAX cine and associated tagged cardiac MR images through time from a control patient.']",Figure 2 Example of SAX and associated tagged cardiac MR images through time from a control patient. Times are displayed from recordings of 25 time-frames.,yes
PMC6124192,Figure_5,oa_package/8b/2d/PMC6124192.tar.gz,['MRI left hip/pelvis: Results are consistent with AVN and early collapse of the femoral head with sclerotic margins.'],Figure 5 MRI left hip/pelvis: Results are consistent with AVN and early collapse of the femoral head with sclerotic margins.,yes
PMC4159772,Figure_4,oa_package/2c/6f/PMC4159772.tar.gz,"['By counting TUNEL+ apoptotic cells throughout entire cortical hemispheres, we see no significant differences between WT and disc/disc embryos (Student s t-test; Supplementary ), further confirming that cell loss is not a contributing factor to the morphological anomalies present in the Wdfy3-deficient cortex.', 'Our analysis revealed that exclusively within the somatosensory area of the disc/disc mutant neocortex heterotopic clusters of cells migrate to superficial laminar positions forming focal cortical dysplasia ().', 'Flp/FRT-mediated recombination produced expected PCR products assessed with Flp primers and absence of lacZ product assessed with lacZ primers (Supplementary ).', 'Homozygous disc mutants exhibit neuronal migration defectsImmunofluorescent analysis of cortical lamination markers Tbr1 (layer VI) and Ctip2 (layer V) reveals abnormalities in layer formation of disc/disc mutants at P0.']",Figure 4 Homozygous mutants exhibit neuronal migration defects Immunofluorescent analysis of cortical lamination markers Tbr1 (layer VI) and Ctip2 (layer V) reveals abnormalities in layer formation of mutants at P0. Arrowheads point to individual focal heterotopia of displaced cells for either marker in the mutant. Asterisks highlight smaller scale lamination anomalies. All sections shown are in the coronal plane of the somatosensory cortex. Scale bar is 200 m.,yes
PMC5453001,Figure_2,oa_package/9b/f6/PMC5453001.tar.gz,"['The appendix was identified to be partially amputed following a previous rupture, distended and filled with mucin ().', '.']","Figure 2. Intraoperative images of (A) the cystic tumor of the ovary and mucinous peritoneal effusion and of (B) the resected ileocecum with a small, partially amputed from a previous rupture, distended appendix filled with mucin (indicated with a white asterisk).",yes
PMC11331705,Figure_1,oa_package/1f/93/PMC11331705.tar.gz,"['Clinical findings revealed no additional craniofacial features or digit abnormalities, despite the aplastic right clavicle and a sloping right shoulder ().', 'Frontal view of the patient showing the absence of the right collarbone comparatively to the left one.', 'A post-procedure chest X-ray confirmed the absence of the right clavicle (), and permitted to rule out a postprocedural pneumothorax.']",Fig. 1 Frontal view of the patient showing the absence of the right collarbone comparatively to the left one.,yes
PMC6222455,Figure_4,oa_package/0f/65/PMC6222455.tar.gz,"['Thus, to examine the effect of NiCur on the levels of H3K27ac, IB analysis was performed on the nuclear extracts from U2OS cells, which were treated with conditions as described in A.', 'However, unlike p53, where blocking of p53K382ac reduced the expression as well as activation of p53, the concentration-dependent decline in the levels of H3K27ac was concomitant with an increase in H3K27me3 (B).', 'The induction of phosphorylation at the H3S28 (H3S28p) site by Dox remains unaltered by NiCur treatment (B).', 'The results show that Dox alone was able to enhance the level of H3K27ac 2-fold (C).', 'The ChIP-qPCR data revealed that treatment with Dox enhanced the enrichment of CBP, in contrast to the treatment with NiCur, which led to the enhancement of EZH2 occupation on the p21 promoter (D).', 'NiCur reprograms chromatin landscape from acetylation, H3K27ac, to trimethylation on lysine 27 of histone H3, H3K27me3.']","Figure 2 Biochemical characterization of compound by cell-based assay and computational methods. ( ) IC was determined by a Dox treated luciferase-driven p53 reporter assay using curcumin and compounds (CPD 2) and 19 (CPD 19) in a dose-dependent manner. RLU stands for relative luciferase units. The IC values were calculated from three technical and biological repeats using the PRISM software; ( ) Chemical structure of a - -curcuminoid-derived compound that has been referred to as NiCur; ( ) Relative inhibition of fluorescence-based in vitro CBP HAT activity in the presence of curcumin and NiCur at a concentration of 5.0 M respectively. The data show mean SEM ( = ), with significance at < 0.05 or 0.01 indicated by * and **, respectively; ( ) NiCur (spheres) binding pose and the peptide inhibitor (sticks) in the active site of CBP/p300 are highlighted and the ribbon diagram depicts the rest of the protein; ( ) The residues of CBP (sticks) interacting with NiCur (spheres).",yes
PMC8274119,Figure_2,oa_package/a3/ea/PMC8274119.tar.gz,"['Based on these findings, a magnetic resonance imaging scan of the chest is performed, which confirms an anterior mediastinal mass ().', '1177_23742895211021980-fig2"" position=""float""/>Questions/Discussion Points, Part 1Describe the 3 Compartments of the Mediastinum and Their Anatomic BoundariesThe mediastinum is defined by 4 boundaries: the thoracic inlet, the diaphragm, the paravertebral sulci, and the pleura.']",Figure 2. T1-weighted magnetic resonance imaging confirms the presence of an anterior mediastinal mass (arrow).,yes
PMC3569958,Figure_6,oa_package/13/89/PMC3569958.tar.gz,"['Surface bound calcium on chitosan, the intracellular calcium and up-regulation of Ca2+-dependent moleculesThe calcium deposition on chitosan after incubation with the medium was examined by SEM, and the respective EDS data are shown in A.', 'The concentration of the free (unbound) calcium remained in the bulk solution and that of the surface bound calcium in the EDTA eluted solution are shown in B.', 'The influences of chitosan on the expression of Ca2+-dependent cell adhesion molecule or surface receptor are further shown in C.', 'The gene expression level of P-selectin (C, Selp) was significantly higher for ASCs on chitosan versus on TCPS.', 'With fluorescent calcium staining, the intracellular calcium of CS1- and CS2-derived ASC spheroids could be visualized by the confocal microscope as shown in D.']","FIG. 6. The surface bound and intracellular calcium associated with the up-regulation of Ca -dependent molecules. Scanning electron microscope/energy dispersive spectrometer (SEM/EDS) data showed the presence of calcium on the surface of chitosan membranes after soaking in the medium for 24h ( CS1; CS2). Quantitative measurements of the free (unbound) calcium remained in the bulk solution and the surface bound calcium in the EDTA eluted solution demonstrated the ability of chitosan to bind more calcium (vs. TCPS). The up-regulation of mRNA expression for Ca -dependent adhesion molecule P-selectin (Selp) as well as the chemokine receptor Cxcr4 after 24h was analyzed by the real-time PCR and normalized to that of the -actin mRNA in the same sample . The increase in the intracellular calcium was visible and quantified by green fluorescence . The data were expressed as meansSD from five independent replicates ( =5). The scale bar represents 20m. *Significant difference, 0.05.",yes
PMC9227275,Figure_2,oa_package/0d/ce/PMC9227275.tar.gz,"['After removing the guided wire, augmentation with PMMA was performed and wound suturing ensued ().', '(a) Operative Procedure, (b) Post-operative CT-scans.']","Figure 2 ( ) Operative Procedure, ( ) Post-operative CT-scans.",yes
PMC11394565,Figure_1,oa_package/53/ad/PMC11394565.tar.gz,"['(Patient with clear disease progression on treatment) shows the progression of the disease for patient 5 (according to the numbering shown in Table 1), specifically the treatment course and CT images (A), the cfDNA (B), the TP53 R175H (c.', '524 G A) mutation (C), and the CA125 (D) levels.', 'On the day of cytoreduction surgery, there were eight mutated copies/mL of plasma (C), and CA125 started to surpass the normal baseline range of 35 U/mL (D).', 'We speculate that the recurrence was caused by a change in subcellular mutated clones, and the increase in cfDNA occurred 400 days before the recurrence was diagnosed (2).', 'Indeed, CA125 began to increase on day 528, and recurrence was diagnosed on day 632 (1).', '2 shows that cfDNA started increasing at 175 days; however, CA125 began increasing on day 532, but the most pronounced growth occurred after day 630.', 'The increase in cfDNA occurred 400 days before the recurrence was diagnosed (2).', '1038/nmeth89816791214\n(A) Summary of patient 5 s clinical information, including the treatment type and dates (treatment dates indicated by the coloured vertical stripes; the date of the cytoreduction surgery is indicated by a red vertical line, and the date of the diagnosed recurrence is indicated by a red cross) as well as computed tomography images and summarised results.', '1Monitoring cell-free DNA (cfDNA) and cancer antigen 125 (CA125) over time in patient 11.', '2Monitoring cell-free DNA (cfDNA) and cancer antigen 125 (CA125) over time in patient 8.']","Figure 1 ( ) Summary of patient 5s clinical information, including the treatment type and dates (treatment dates indicated by the coloured vertical stripes; the date of the cytoreduction surgery is indicated by a red vertical line, and the date of the diagnosed recurrence is indicated by a red cross) as well as computed tomography images and summarised results. ( ) Cell-free DNA (cfDNA) plasma levels are presented as ng/mL. ( ) Circulating tumour DNA (ctDNA) plasma levels are expressed as the number of mutated copies/mL of plasma. Replicates were used to quantify the average R175H copies for each time point, and the error bars represent the standard deviation across replicates; no bar indicates that the standard deviation was too low to be visualised on the scale used. The volume-adjusted limit of detection was 21.2 GEs (genome equivalent)/mL for all time points. A two-tailed -test was used to compare the ctDNA levels between sequential time points. ( ) Cancer antigen 125 (CA125) serum levels (U/mL); data from multiple replicates were not provided. * = 0.05.",yes
PMC7491956,Figure_7,oa_package/ad/18/PMC7491956.tar.gz,['.'],Figure 7. Irregular hyperintensity of the primary lesion in DWI (b=800 sec/mm ).,yes
PMC9596221,Figure_2,oa_package/61/13/PMC9596221.tar.gz,"['After treatment with various concentrations of 2JY-OBZ4 and 10 M Hup-A for 24 h, western blot results showed that GSK-3 was increased in all transfected groups, and there was no significant change observed (A, 2B).', 'Furthermore, there was no difference on tau level among all groups (A, 2C).', 'Interestingly, 16 M and 64 M 2JY-OBZ4 decreased tau hyperphosphorylation of Thr181 site; 4 M and 64 M 2JY-OBZ4 decreased tau hyperphosphorylation of Ser396 site (A, 2D).', 'As positive control, Hup-A decreased tau phosphorylation of Ser396 site (A, 2D).', '2JY-OBZ4 resisted tau hyperphosphorylation in HEK293-hTau cells transfected with GSK-3 plasmid.']","Figure 2 ( ) Western blots and ( ) quantitative analysis for GSK-3, Tau, tau-pT181 and tau-pS396 in HEK293-hTau cells overexpressed with GSK-3. MW Molecular weight. n = 3 per group. NS means no significance, p value significance is calculated from a one-way ANOVA, data are represented as mean SEM. *p < 0.05 and ##p < 0.01.",yes
PMC7176599,Figure_1,oa_package/37/e2/PMC7176599.tar.gz,"[' 1a) and fat mass (', ' 1b), leading to significant difference between saline and DOX-treated mice.', ' 1a), an effect which was not seen in fat mass gain (', ' 1b).', ' 1c).', '', 'a Two-way ANOVA genotype and treatment effects, b two-way ANOVA treatment effect, c Tukey post hoc test following two-way ANOVAEchocardiography AnalysisEchocardiography was conducted at week six.']","Fig.1 Body weight and composition in saline and doxorubicin (DOX) -treated wild-type (WT) and NPY-OE (NPY-OE) mice ( =79/group), body weight gain in 6weeks, fat mass gain, and lean mass gain. Values are presented as meansSEM. *** <0.001 DOX vs. saline, ## <0.01 NPY vs. WT. Two-way ANOVA genotype and treatment effects, two-way ANOVA treatment effect, Tukey post hoc test following two-way ANOVA",yes
PMC10795236,Figure_6,oa_package/47/ef/PMC10795236.tar.gz,['\nDISCUSSION\n\nPulmonary papillomas are rare benign tumors of the\nlung and have been reported as case reports in the\nliterature.'],Figure 6 Presence of papillary projections in the polyp.,yes
PMC10657827,Figure_4,oa_package/5f/95/PMC10657827.tar.gz,"['4A).', '4A).', 'TDP and ubiquitin pathology in mVCP mouse brain is improved with arimoclomol treatment.', 'DAPI labels nuclei (blue) in all imagesWe next examined the expression of ubiquitin in the brain of 14-month old mVCP mice.', '4B).', '4C).']",Fig. 4 TDP and ubiquitin pathology in mVCP mouse brain is improved with arimoclomol treatment. ( ) TDP-43 localisation in cortical cells. Insets show cells at higher magnification. Scale bar = 20m. ( ) Ubiquitin immunoreactivity in mouse cortex shows ubiquitin-positive aggregates in mVCP brain (red). Scale bar = 20m. ( ) Stress granule marker Tia1 colocalised with TDP-43 and ubiquitin in mVCP brain. Scale bar = 10m. DAPI labels nuclei (blue) in all images,yes
PMC6818368,Figure_3,oa_package/eb/fc/PMC6818368.tar.gz,['Histopathologic findings at 20 magnification.'],Fig 3 Histopathologic findings at 20 magnification. Numerous scattered large angulated multinucleate cells and fibrohistiocytic cells.,yes
PMC7112039,Figure_4,oa_package/d8/0d/PMC7112039.tar.gz,"['These cells were also observed in the lymphoreticular system including the spleen (C), tonsil, and lymph node.', '4C), hepatic centrilobular necrosis, acute renal tubular necrosis, microglial nodules with demyelination in cerebral white matter (E and F).', '4A and B), activated lymphoid cells in reticuloendothelial system, lymphoid depletion, skeletal muscle fiber necrosis (D), acute renal tubular necrosis, normal brain[4]4F/262004/Thailand10DAD, interstitial pneumonia, cholestasis, hemophagocytic activity in liver, lymphoid depletion in spleen[27]5M/62004/Thailand17Consolidated lungs with focal hemorrhage, DAD, interstitial lymphoplasmacytic infiltrate, bronchiolitis, histiocytosis in lymphoid system but no hemophagocytosis, reactive hepatitis, small foci of necrosis in brain[28], [29]6, 7, 8Not specified2004/ThailandNot specifiedLung and spleen examined only, DAD with hyaline membrane, reactive fibroblasts and hemorrhage, atypical lymphocytes in spleen.', 'Extrapulmonary pathology of H5N1.', 'Reactive hemophagocytic syndrome was noted in 4 cases (cases 1-4) (A and B).', 'Large activated lymphoid cells were present in the lymphoreticular system (A and C inset).', 'The spleen showed atrophic white pulp and congested red pulp (C).', 'Skeletal muscle fiber necrosis was documented in 2 cases (cases 1 and 3) (D).', 'In the 13-year-old girl dying at 1 month (case 1), the cerebral white matter showed scattered microglial nodules with hemorrhage, rarefaction, demyelination, axon balls, and foamy or pigment-laden macrophages (E and F).']","Fig. 4 Extrapulmonary pathology of H5N1. A, Reactive hemophagocytosis in pulmonary hilar lymph node and 3 large activated lymphocytes in the center of field. B, Reactive hemophagocytosis in bone marrow. C, Lymphoid depletion in spleen and large activated lymphocytes in inset. D, Skeletal muscle fiber necrosis. E, Microglial nodules with rarefaction and demyelination in cerebral white matter. F, High-power view of microglial nodule with a few axon balls in the lower field and foamy and pigment-laden macrophages (hematoxylin-eosin stain, original magnification 1000 [A, B, and C inset], 100 [4E], 400 [all others]; A-D taken from a patient with H5N1 dying on day 10 [case 3 of ]; E and F taken from a patient with H5N1 dying on day 30 [case 1 of ]).",yes
PMC9616497,Figure_2,oa_package/61/03/PMC9616497.tar.gz,"['We established a highly sensitive flow cytometric assay that uses high-density conjugation of Asialo-BSM to microbeads (A).', 'We created a standard titration curve using purified anti-Tn IgM from healthy sera (B).', 'There were no significant differences in total serum IgM in control versus IgAN samples (C).', 'SDS-PAGE immunoblots probed for IgM, IgG, and IgA of the purified anti-Tn antibodies showed consistent results, demonstrating elevations of IgM to the Tn antigen (D).', 'In addition, we measured the titer of serum IgA1 levels and its percentage of Tn positivity using Helix pomatia agglutinin (HPA) microbeads (E).', 'Serum IgA1 levels were elevated in patients with IgAN as compared to healthy controls as expected (F, left), but the percentage of Tn positivity of IgA1 in serum did not change in both groups (', '.']","Fig. 2. Elevated level of anti-Tn antibodies and total IgA1 in patients with IgAN. ( ) Depiction of anti-Tn IgM detection directly from human sera using Asialo-BSM microbeads, similar to Tn(+)matrix beads in fig. S1A, flow cytometry using Alexa Fluor 488labeled anti-human IgM for signal detection. ( ) Plot of standard curve of purified anti-Tn IgM from human sera and isotype human IgM serves as a control. ( ) Sera with IgAN (P1 to P20) or control (C1 to C20) analyzed by flow cytometry using Asialo-BSM microbeads. Donor information listed in . Serum IgM analyzed using enzyme-linked immunosorbent assay (ELISA). Box plot represents three independent experiments (control, = 20; IgAN, = 20; , male; , female). Students test, *** = 1.3 10 ; n.s. (not significant), = 0.07. ( ) Affinity-purified anti-Tn antibodies of parallel experiments, as in , from healthy control (C2) and patient with IgAN (P1) were loaded as indicated on top of the immunoblots, and immunoblots were probed for IgM, IgG, and IgA antibodies as indicated on the right. ( ) Depiction of Tn(+)IgA1 detection from human serum using HPA microbeads by flow cytometry using fluorescein isothiocyanate (FITC)labeled anti-human IgA1 for signal detection. ( ) Sera with IgAN (P1 to P20) or control (C1 to C20) were analyzed by flow cytometry using HPA microbeads. Purified Tn(+)IgA1 and Tn()IgA1 were used as 100 and 0%, respectively. Serum IgA was analyzed using ELISA. Box plots represent three independent experiments (control, = 20; IgAN, = 20; , male; , female). Students test, ** = 2.0 10 ; n.s., = 0.10.",yes
PMC6154927,Figure_4,oa_package/c4/29/PMC6154927.tar.gz,"['4a).', '4a, that BACE1 was indeed present in the ipsilateral white matter tracts of 18 mo stroked C57BL/6 mice compared to their age-matched sham counterparts at 12 weeks post-surgery.', 'Stroke induces -secretase (BACE) 1 and neuregulin (NRG) 1 type III expression in the white matter tracts of aged wildtype (wt) mice compared to young adult mice.', '05To support the hypothesis that BACE1 and NRG1 type III are part of a chronically activated myelin repair mechanism following stroke, we then tested if NRG1 type III expression is chronically increased in stroked wt mice, and if it colocalizes with areas of BACE1 expression and A 42 deposition.', '4b).', '4b, that NRG1 type III was present in the ipsilateral white matter tracts of the 18 mo stroked C57BL/6 mice compared to their age-matched sham counterparts at 12 weeks post-surgery.', '4c), and the Fluoro-Jade staining revealed a delayed and sustained area of degenerating axons in the ipsilateral white matter tracts (', '4d).', '4a).', '4b show that NRG1 type III expression is present in the white matter tracts of the ipsilateral hemisphere of aged wt mice at 12 weeks post-stroke, and here, we determined the chronic impact of stroke on NRG1 type III expression in aged hAPP-SL mice.']","Fig. 4 Stroke induces -secretase (BACE) 1 and neuregulin (NRG) 1 type III expression in the white matter tracts of aged wildtype (wt) mice compared to young adult mice. Western blotting with a BACE1 (~70 kDa) or NRG type III (~50 kDa) antibody detected a single band at the appropriate molecular weight in mouse brain lysates for each protein. Representative 20 images (n=3 mice/experimental group) of ( ) BACE1+ and ( ) NRG1 type III+ immunostaining (arrows) in the white matter tracts (thalamus-internal capsule) of 18 mo mice at 12 weeks after sham or stroke surgery (Equivalent = area imaged in wt-sham mice that is equivalent to the ipsilateral hemisphere imaged in wt-stroke mice; Contralateral = area imaged in the contralateral hemisphere of wt-stroke mice that is equivalent to the ipsilateral hemisphere of wt-stroke mice). Scale bar, 50 m (BACE1 and NRG1 type III). Images revealed BACE1 and NRG1 type III expression in the ipsilateral white matter tracts of stroked, but not in sham-operated mice. Representative 10 immunofluorescence images (n=3 mice/experimental group) of total tau+ immunostaining in the ipsilateral and contralateral white matter tracts of wt mice after stroke surgery. Scale bar, 125 m. Image: Representative 40 Fluoro-Jade staining in the ipsilateral white matter tracts of wt mice after stroke confirms that this is an area of axonal degeneration following DH stroke. Scale bar, 100 m. Graph: Relative to nave mice, there is significant Fluoro-Jade staining starting at 1 week (wk) post-stroke, continuing into at least 8 wk post-stroke. Data represent mean SEM from n=5 mice/experimental group. *p<0.05",yes
PMC4409798,Figure_2,oa_package/5d/15/PMC4409798.tar.gz,"['Two perpendicular lines (AA and BB) were drawn on the image of the impacted teeth, of which one line passed through the center of the crown and the other line passed through the long axis of the tooth ().', 'Two perpendicular lines (AA and BB) drawn on the image of the impacted teeth, of which one line passes through the center of the crown and the other line passes through the long axis of the tooth.']","Figure 2 Two perpendicular lines (AA and BB) drawn on the image of the impacted teeth, of which one line passes through the center of the crown and the other line passes through the long axis of the tooth.",yes
PMC6563000,Figure_8,oa_package/56/ec/PMC6563000.tar.gz,"['Second dose of Hamp was administered 5 h later and tissue was harvested 9 h after the surgery (\nA\n).', 'However, mice that received Hamp therapy within 30 min of CLP displayed significantly lower bacteremia (\nB and C\n) and AKI (as measured by renal NGAL and KIM gene expression) (\nD and E\n).', 'Hamp reduces bacteremia and AKI when administered after the onset of sepsis.', ', as indicated in \nA\n within 20-30 mins post CLP, followed by another dose 5 hrs later significantly reduced bacteremia in mice (B-C).']","Figure 8 Hamp reduces bacteremia and AKI when administered after the onset of sepsis. Study design and therapeutic treatment of Hamp in CLP settings . Hamp injection (100g/mouse, I.P., as indicated in within 20-30 mins post CLP, followed by another dose 5 hrs later significantly reduced bacteremia in mice . Kidney tubular injury as measured by renal gene expression of NGAL and KIM-1 was also significantly reduced in this group. *P < 0.05, ***P < 0.0005. Data points are plotted as mean SEM (n = 4-5 per group). Experiments were repeated twice and representative data from a single experiment is depicted.",yes
PMC6963574,Figure_6,oa_package/26/9f/PMC6963574.tar.gz,"['iNOS protein expression was strongly detected by 4 dpi, and remained elevated in the lungs it was sustained at least until 14 dpi in STAT6 / mice (a,b).', 'In contrast, STAT1 / mice had reduced expression of iNOS, while WT mice displayed discrete and transient iNOS expression, particularly at 4 dpi (a,b).', 'M1 macrophage activation marker in lung tissue assessed by immunofluorescence.']","Figure 6 M1 macrophage activation marker in lung tissue assessed by immunofluorescence. Microscopy data of lung sections from WT, STAT1 and STAT6 mice at 4, 7 and 14 dpi, stained with the DNA-binding dye (DAPI) in blue and iNOS ( ) in green. Quantification of the fluorescent intensity of iNOS ( ) in lung sections stained from a, in WT (white bars), STAT1 (gray bars) and STAT6 (black bars) mice. Photographs were taken with a 20 objective. Data are shown from two independent experiments as the mean SEM (n = 6 per group). Two-way ANOVA with Tukeys multicomparison test. * < 0.05 comparing WT versus STAT1 and STAT6 , and STAT1 versus STAT6 mice at the same time point of infection.",yes
PMC8577366,Figure_6,oa_package/2e/79/PMC8577366.tar.gz,"['Predictive clustering\nc shows the distribution of inferred EM values of two ROIs of a typical sample, and the tissue structure visible in the H E image has a clear influence on the shape of these distributions.', 'Liver tissue sample G340-03-02 classified as tumour .', ' shows a sample excised from patient G340 with two ROIs shown in more detail, including histograms of the predicted EM values for each of these regions.', 'While the heterogeneity of the inferred EM may be visually apparent in the tumour samples, it is the distribution of values that contains relevant, quantifiable signatures of the underlying tissue pathology, as shown in the inset histograms in c.', 'The predicted whole-sample EM distributions shown in c and 7 differ in shape from those observed in previous works however, where thick ( 1 mm) tissue sections were measured with AFM cantilever tips tens of nm in size, resolving individual cells and collagen fibres.', 'c shows in particular how the network is able to predict regions of high EM that correspond to increased levels of the fibrous ECM protein collagen as evident in the stained image, despite an absence of obvious features in the unstained image.']","Fig. 6 Liver tissue sample G340-03-02 classified as tumour. (a) Shows unstained section and (b) shows stained section (scale bars 1 mm). (c) Shows network prediction of EM values with (a) as input. Insets: unstained and stained ROIs (scale bar 250 m). Histogram in bottom left shows distribution of predicted EM values for each ROI. = 350, 166 for narrower distribution in yellow and = 313, 293 for broader distribution in cyan.",yes
PMC9585901,Figure_3,oa_package/bb/d0/PMC9585901.tar.gz,"['This CTA was reconstructed to create a 360 VR (360VR) model using a VR surgical planner (SRP; Surgical Theater, Cleveland, OH, USA; , see Supplementary material online, Video S1).', 'A 29 mm SAPIEN 3 valve (Edwards Lifesciences, Irvine, CA, USA), selected based on the already implanted MV, was placed inside the bioprosthetic MV and analysed in VR at different angles (B).', 'Preoperative computed tomography.', '4 Here, SAPIEN 3 was virtually placed over the existing bioprosthetic MV in VR (B, see Supplementary material online, Video S1).']","Figure 3 Preoperative computed tomography. ( ) DICOM images in sagittal and coronal views of the mitral valve and left-ventricular outflow tract. ( ) Snapshots of the 360 VR model with the virtual 29-mm SAPIEN 3 showing left-ventricular outflow tract opening and neo-left-ventricular outflow tract measurements in virtual reality. ( ) While the 360 VR snapshots clearly show the left-ventricular outflow tract opening, the evaluation of the model in virtual reality was necessary to better assess its exact surface area due to the lack of depth perception in 2D images.",yes
PMC6122529,Figure_2,oa_package/04/c0/PMC6122529.tar.gz,"[""VivaScope 1500 () is a wide-probe microscope which uses a ringed glass window that is fixed to the patient's skin and then coupled to the RCM camera, which is attached to a movable steel arm."", '.']",,yes
PMC6269332,Figure_13,oa_package/00/2d/PMC6269332.tar.gz,[],Fig. 13 A 3-year-old boy with fever and abdominal pain is studied. US shows a cystic mass ( ) with internal debris and next to an ileal loop ( ). The lesion ( ) is surrounded by echogenic mesenteric fat (*) as an inflammatory sign. Surgical findings: a 5-cm ileal complicated duplication cyst was found with gastric mucosa with haemorrhagic and ulcerated walls,yes
PMC9388999,Figure_1,oa_package/0a/d5/PMC9388999.tar.gz,['MRI findings.'],"Figure 1 MRI findings. MRI revealed a round mass (arrows) with a clear edge in segment 2 of the liver. It showed low signal intensity on T1WI and high signal intensity on T2WI (A,F,B). The fat component was not demonstrated (C). DWI MRI showed a ring of high intensity (D). The rim of the lesion appeared slightly hypointense to the surroundingparenchyma on ADC maps (E). Gd-DTPA dynamic enhanced scanning showed a prolonged ring enhancement pattern (G-I).",yes
PMC10314548,Figure_1,oa_package/08/83/PMC10314548.tar.gz,"['A focused dermatological examination was significant for diffuse violaceous plaques and erosions with adherent haemorrhagic crusting on her face, bilateral upper and lower extremities, and dorsal surfaces of her hands (figure 1).', 'Violaceous plaques and erosions involving the right hand (top) and right upper extremity (bottom) at the time of admission.']",Figure 1 Violaceous plaques and erosions involving the right hand (top) and right upper extremity (bottom) at the time of admission.,yes
PMC2842180,Figure_11,oa_package/4a/af/PMC2842180.tar.gz,[],"Figure 11 Infantile haemangioma. (A) Axial T2-weighted MRI shows bilateral superficial, plaque-like facial haemangiomas (arrows) in a 10 month-old girl. (B) The same lesion shows uniform enhancement on an axial T1-weighted sequence following gadolinium administration.",yes
PMC6221925,Figure_5,oa_package/3c/13/PMC6221925.tar.gz,['The NIRS-SPM T-statistic maps in the control (A C) and modified (D F) sessions.'],"Figure 5 The NIRS-SPM T-statistic maps in the control and modified sessions. Activation map in the early and late phases of the control session, and in the early and late phases of the modified session are shown. Normalized group results of the subtraction of the early phase from the late phase in the control and modified sessions are also shown. Colored bars indicate the -values of active voxels. Green dotted circles indicate area Spt (posterior part of the planum temporale). L-ROL, left Rolandic operculum; L-STG, left superior temporal gyrus; L-PoCG, left postcentral gyrus; L-SMG, left supramarginal gyrus; L-IFGoperc, left opercular part of the inferior frontal gyrus; L-PreCG, left precentral gyrus; L-ANG, left angular gyrus.",yes
PMC5217947,Figure_3,oa_package/5b/00/PMC5217947.tar.gz,['Infliximab and human immunoglobulin (Ig)G cause significant apoptosis in Trichuris muris infected mice.'],"Figure 3 Infliximab and human immunoglobulin (Ig)G cause significant apoptosis in infected mice. Colitic mice were treated with infliximab (IFX), and assessed for apoptotic cells [terminal deoxynucleotidyl transferase dUTP nick end labellingfluorescein isothiocyanate (TUNELFITC)] in proximal colon 10 days after single treatment day 35 postinfection. (a) Uninfected untreated; (b) uninfected IFXtreated; (c) infected rat IgGtreated; (d) infected antimouse tumour necrosis factor (TNF); (e) infected human IgGtreated; (f) infected IFXtreated. Micrographs representative of two independent experiments; =5 in each group. (a,b) Scale bar 500 m; (cf) scale bar 200 m. [Colour figure can be viewed at ]",yes
PMC10604421,Figure_2,oa_package/35/be/PMC10604421.tar.gz,[' Color Doppler ultrasound image of the left breast palpable mass demonstrates mild internal vascularity.'],Figure 2 Color Doppler ultrasound image of the left breast palpable mass demonstrates mild internal vascularity.,yes
PMC5558110,Figure_2,oa_package/28/35/PMC5558110.tar.gz,['Computed tomography arterial and venous phases showing a pseudoaneurysm in a patient with necrotizing pancreatitis.'],Figure 2 Computed tomography arterial and venous phases showing a pseudoaneurysm in a patient with necrotizing pancreatitis.,yes
PMC10421797,Figure_24,oa_package/1f/fe/PMC10421797.tar.gz,[],"Fig. 24 Sagittal ( )and axial T1 post-gadolinium( ) magnetic resonance imaging (MRI)in a 6-year-oldgirl with tuberculous (TB) meningitis show enhancement of the arachnoid with enhancement and clumping ofthe nerve roots. Sagittal T1 post-contrastMRI in a different patientwith TBmeningitis, a 4-year and-10-month old girl who had repeated failed lumbar punctures, shows the spinalcanal to be completely ocupied by enhancing tissue ( )",yes
PMC11534639,Figure_2,oa_package/da/6d/PMC11534639.tar.gz,[],FIGURE 2 A 17yearold female with metastasized pancreatic neuroendocrine tumor. CT image of the liver metastases in the plain phase. Showing multiple welldefined heterogeneous masses in the liver hemilobes.,yes
PMC10705997,Figure_1,oa_package/8a/13/PMC10705997.tar.gz,"['over several durations of contact, we observed increases in these cells at 48, 72, and 96 h after the exposition in both the control and patients compared to levels at zero hours B,C (controls 7.', 'However, when we compared data without considering the time of incubation (48, 72, and 96 h) between the two tested microorganisms, no significant differences between the groups analyzed were found (D, p = 0.', '05, E.', '05, F.', 'The flow cytometry staining strategy is shown at the beginning of the figures, with a representative example in A.', '17309032234529\nPercent of CD14+ cells after stimulation with endodontic microorganisms.']","Figure 1 Percent of CD14 cells after stimulation with endodontic microorganisms. The culturing of PBMC and endodontic bacteria was performed at different times, and staining was performed with flow cytometry analysis, as indicated in the . ( ) Representative dot plots of a healthy subject (control) at 0 and 48 h of stimulation with endodontic microorganisms, showing the histograms corresponding to CD14 cells stained with isothiocyanate of fluorescein (FITC). ( ) Percentages of CD14 cells in controls and patients with primary infections, stimulated with spp. at 0, 48, 72, and 96 h of culture. ( ) Percentage of CD14 cells in controls and patients with primary infections, stimulated with spp. at 0, 48, 72, and 96 h of culture. ( ) Comparison of percentage of CD14 cells in controls and patients with primary infections stimulated with spp. and spp. at all the tested incubation times. ( ) Percent of CD14 cells in controls and patients with primary infections, stimulated with spp. at 0, 48, 72, and 96 h of culture. ( ) Percent of CD14 cells in controls and patients with primary infections, stimulated with spp. at 0, 48, 72, and 96 h of culture. ( ) Data correspond to the median and interquartile range, = 25, * < 0.05. ** < 0.01. *** < 0.001.",yes
PMC7759851,Figure_4,oa_package/3e/88/PMC7759851.tar.gz,"['A total of 20 ml of blood-tinged transudate fluid was collected from the thoracic cavity and both lungs showed lobar areas of reddish consolidation, as well as sparse subpleural soft to firm greyish/whitish slightly raised nodules (a).', 'The heart weighted 12 g and marked enlargement of the right atrial and ventricular cavities was evident (b).', 'Gross pathological findings from the examined kitten.']","Figure 4 Gross pathological findings from the examined kitten. ( ) Lungs showed diffuse hyperaemia (orange arrow) with lobular areas of consolidation (white arrow) and sparse whitish, slightly raised nodules. ( ) Marked right-sided cardiac enlargement was evident (white arrow).",yes
PMC9472137,Figure_2,oa_package/44/7e/PMC9472137.tar.gz,"['Axial MRI of the abdomen further delineating the infiltration extension into the liver.', '5 cm in the superior pole of the kidney and contained a cyst measuring 2 cm, filled with fibrinopurulent material (figure 2).']",Figure 2 Axial MRI of the abdomen further delineating the infiltration extension into the liver.,yes
PMC4901117,Figure_1,oa_package/07/3d/PMC4901117.tar.gz,"[' 1a,b) and then established that body weights were normal in PLB4 mice aged up to 4 months (', ' 1c) but decreased compared with wild-type (WT) controls at 5 and 8 months.', ' 1d,e).', ' 1f), while lean mass was unaffected (', ' 1g), as demonstrated by EchoMRI.', ' 1h k) and impaired GSIS from 4 months of age (', ' 1m,n).', ' 1l), despite continued hyperglycaemia.', ' 1o).', 'Systemic diabetes in neuronal hBACE1 knockin mice.', '001Altered plasma metabolic homeostasis and hyperleptinaemiaLevels of the adipocyte-derived hormone, leptin, which strongly correlate with obesity and diabetes, were initially normal in PLB4 mice, but were drastically elevated at 4 and 8 months (', ' 1p) when adiposity was increased.', ' 1q).']","Fig. 1 Systemic diabetes in neuronal knockin mice. ( ) BACE1 protein content in soluble lysates from neuronal and pancreatic tissues from PLB4 and WT mice. ( ) BACE1 protein screening in other tissue types from PLB4 mice only. ( ) Body weight of WT and PLB4 mice at 3, 4, 5 and 8months (m) of age. ( , ) Normalised food ( ) and water ( ) intake in 5- and 8-month-old mice. ( , ) Body composition data obtain from EchoMRI scans showing adipose ( ) and lean mass ( ) in mice aged 4, 5 and 8months. ( ) GTTs at age 3 ( >0.05), 5 ( <0.01) and 8 ( <0.05) months (m). ( ) Total glucose excursions during GTTs. ( ) Fasted serum insulin concentrations in 5- and 8-month-old PLB4 and WT mice. ( ) GTTs in 4-month-old mice ( <0.001; onset of defective glucose disposal). ( ) GSIS in 4-month-old mice during GTTs. ( ) Serum NEFA at 3 and 8months of age. ( ) Fasted serum leptin levels in PLB4 vs WT mice at 3, 4 and 8months of age. ( ) Serum markers detected in 8-month-old PLB4 mice using an enzymatic multiplex assay. White bars, WT mice; black bars, PLB4 mice. Data represent means + SEM or ( , ) means + SEM normalised to WT values. * <0.05, ** <0.01, *** <0.001",yes
PMC5836362,Figure_2,oa_package/be/51/PMC5836362.tar.gz,"[' 2).', 'Photographs showing the dual-room sliding CT scanner system with interventional radiology features.', 'When we perform emergency surgery or interventional radiology for a severely injured or ill patient in the regular emergency room, the sliding CT scanner is moved to the new adjacent CT suite with the radiolucent table, and we can perform CT scanning of another in/outpatientConclusionWe report a new workflow concept using a dual-room sliding CT scanner system with interventional radiology features.']","Fig. 2 Photographs showing the dual-room sliding CT scanner system with interventional radiology features. The new CT suite has another radiolucent table. When we perform emergency surgery or interventional radiology for a severely injured or ill patient in the regular emergency room, the sliding CT scanner is moved to the new adjacent CT suite with the radiolucent table, and we can perform CT scanning of another in/outpatient",yes
PMC3145845,Figure_2,oa_package/6b/7e/PMC3145845.tar.gz,['Schematic diagram of the '],"Fig. 2 Schematic diagram of the . PT, pronator teres muscle (cutting); ME, medial epicondyle; FCR, flexor carpi radialis muscle; PL, palmaris longus musle; FDS, flexor digitorum superficialis musle; FCU, flexor carpi ulnaris muscle; AFCU, accessory flexor carpi ulnaris musle; PA, palmar aponeurosis; FR, flexor retinaculum; P, pisiforme bone.",yes
PMC10366931,Figure_8,oa_package/b1/b3/PMC10366931.tar.gz,"['MRI shows enlargement and an increased enhancement of CNs and foramina with various degrees of increased T2 signal, along with a loss of perineural fat on T1-weighted sequences () [35].', 'Indirect perineural invasion of the skull base by a previously surgically resected parotid adenoid cystic carcinoma.']","Figure 8 Indirect perineural invasion of the skull base by a previously surgically resected parotid adenoid cystic carcinoma. ( , ) Axial T2W image shows asymmetric infiltration of the inferior aspect of the right cavernous sinus, Meckels cave, and inferior orbital fissure by a hypointense soft tissue lesion (white arrows) extending into the right prepontine cistern along the trigeminal nerve cisternal segment (black arrows). ( ) Coronal postcontrast T1W image demonstrates infiltrative heterogeneous enhancement in the right masticator and parapharyngeal spaces and a thickened enhancing right mandibular nerve (blue arrow), which provides a pathway for the perineural intracranial spread of the tumor. ( ) Axial postcontrast T1W images show heterogenous enhancement of the perineural tumoral infiltration in the foramen ovale (blue arrow), inferior orbital fissure, Meckels cave, and right trigeminal nerve cisternal segment (white arrows).",yes
PMC6736986,Figure_4,oa_package/5b/2f/PMC6736986.tar.gz,"['T Cell Infiltration and AAA SizeFinally, to ascertain if T cell infiltration is associated with AAA stage/severity, we stratified T cell content in both wall and PVT according to AAA diameter ().', 'Relationship between T cell number and AAA size.']",Figure 4 Relationship between T cell number and AAA size. AAA diameter was determined by preoperative CT. Graphs display relationship between AAA size (tertile 1 53 mm; tertile 2 60 mm; tertile 3 > 60 mm) and CD3 cell infiltration in wall and PVT (mean/SEM/2575 CI); ( = 20/10/9 for tertiles); statistical comparisons were performed by ANOVA with Neuman-Keuls analysis.,yes
PMC7881375,Figure_9,oa_package/4f/81/PMC7881375.tar.gz,"['5 mm stainless steel splint ().', 'Photographic image showing teeth after surgical extrusion (Case 2)After surgery, Augmentin (625 mg, 2*1), a penicillin-derived antibiotic, a mouthwash containing chlorhexidine, Parol, an analgesic agent, were prescribed for 7 days to the patient against the risk of pain and infection.']",Figure 9 Photographic image showing teeth after surgical extrusion (Case 2),yes
PMC5977414,Figure_3,oa_package/de/13/PMC5977414.tar.gz,"['3FA exposure elevated the gene expression of CXCL1 and TNF-alpha in the lung tissue of fibrotic mice (Panels A and E) shows that exposure to FA in fibrotic mice increased the gene expression of CXCL1 (F + FA group 67.', 'FA exposure elevates the gene expression of CXCL1 and TNF-alpha in the lung tissue of fibrotic mice.', 'In panels B, C and D no differences were observed among the groups of study.']","Fig. 2 Groups of mice were submitted to bleomycin injection (1.5U/kg, orotracheal route) and after 7 days were exposed to FA inhalation (0.75ppm, 1h/day, 15 days). Non-manipulated animals were used to basal parameters. After 24h of last exposure to FA or 14 days after fibrosis development, the cytokines were determined. Data represent the meanSEM of 6 animals. * <0.05 compared to the B group; <0.05 compared to the F group; <0.05 compared to the FA group.",yes
PMC9526651,Figure_6,oa_package/5a/16/PMC9526651.tar.gz,"['These changes may be related to apoptosis caused by the autophagic response of cells in the stress state ((a) 6(d)).', '005"" position=""float""/>Ultrastructural changes in ER.']",Figure 6 Ultrastructural changes in ER. (a) Ultrastructure in control rats. (b) Ultrastructure in one-month modelling rats. (c) Ultrastructure in three-month modelling rats. (d) Ultrastructure in five-month modelling rats. : ER; : apoptotic body; : vacuolation.,yes
PMC9741155,Figure_1,oa_package/b5/a7/PMC9741155.tar.gz,"['Radiographic projections (A,B) revealed the presence of 5 eggs located at the right side of the caudal coelomic cavity.', 'A generalized soft tissue opacity occupying the entire coelomic cavity was noted, causing obvious compression of the lung field on the laterolateral (B) and craniocaudal projection.', '1 cm (C) at the left-mid- to caudal coelomic cavity.', 'The histological examination of the ovarian mass revealed a cell-rich tissue that was growing infiltrative in dense collagen stroma (D).', '18828Chronic egg binding associated with a scirrhous ovarian carcinoma in a red-bellied slider (Trachemys cripta elegans).']",Figure 1 Chronic egg binding associated with a scirrhous ovarian carcinoma in a red-bellied slider ( ). ( ) Dorsoventral radiographic projection: 5 eggs located at the right side of the caudal coelomic cavity. An irregular shape is noted in 3 eggs and all eggs show abnormally thickened shells with a lamellar appearance in the most cranial egg. One egg has an unusual small size and is fused with one of the other eggs (arrow). ( ) Generalized soft tissue opacity of the coelomic cavity with obvious compression of the lung field on the left laterolateral projection. ( ) Postoperative view after removal of the ovarian neoplasia and retained eggs. ( ) Histological section of a scirrhous ovarian carcinoma (Hematoxylin and eosin stain) composed of a cell-rich tissue that is growing infiltrative in dense collagen stroma. Neoplastic cells are organized in islets to multi-layered tubules.,yes
PMC5573554,Figure_2,oa_package/1b/4a/PMC5573554.tar.gz,"['Magnetic resonance imaging (MRI) of the brain at 22 hours of life showed diffusely abnormal cerebral cortical/subcortical diffusion restriction which may be secondary hypoxic-ischemic injury (\n\n).', 'org/1999/xlink"" xlink:href=""10-1055-s-0037-1605372-i160079-1""/>\nMagnetic resonance imaging of the brain showing diffusely abnormal restricted diffusion throughout the cerebral cortex involving the bilateral frontal, bilateral parietal, left greater than right occipital, and temporal regions, predominantly in the cortex and subcortical white matter.']","Fig. 2 Magnetic resonance imaging of the brain showing diffusely abnormal restricted diffusion throughout the cerebral cortex involving the bilateral frontal, bilateral parietal, left greater than right occipital, and temporal regions, predominantly in the cortex and subcortical white matter. The edema in this image shows the event was a global insult.",yes
PMC9374061,Figure_4,oa_package/39/64/PMC9374061.tar.gz,[],"FIGURE 4 . ( ) To guide the singlecell mapping of SARSCoV2 infection by cell type, we generated and integrated singlecell RNA sequencing datasets from gingiva, mucosa, and minor salivary glands (9 samples; 13,824 cells). Of the 34 unique cell types (epithelial, mesenchymal, and immune cell populations), the only cells that expressed the indicated genes involved in SARSCoV2 entry were barrier epithelial cells. The abundance of the indicated transcripts within each of the barrier epithelia populations colored according to their presence in males and females is shown. See Huang et al. for detailed methods. ( ) Visualization of mRNA for the indicated SARSCoV2 entry factors was performed with RNAscope (ACD) in situ hybridization of healthy human gingival tissues. The transcripts for the indicated entry factors were enriched in junctional epithelia (JE) and sulcular epithelia (SE), especially in the most terminally differentiated and shedding populations (indicated with asterisks) in the gingiva and oral mucosa. AG, attached gingiva. (See Methods for details.) ( ) The sites of SARSCoV2 infection in the oral cavity. Infection is indicated by the blue glow. Only sites of known infection are labeled. ( ) SARSCoV2 infection and the immune response in a human salivary gland. Epithelial cells (ducts and acini) were detected with an antibody to all cytokeratins (panCK); infection and replications with in situ hybridization (red and green, respectively). The heterogeneity of gland infection was shown with immunohistochemistry, and arrowheads indicate spike protein staining. Sialadenitis was diagnosed by a team of pathologists; infiltrating T cells were detected with a clinical antibody panel. [Credit for panel C: Heather McDonald, BioSerendipity, LLC, Elkridge, MD]",yes
PMC8638086,Figure_10,oa_package/55/5d/PMC8638086.tar.gz,[],"Figure 10. - Same b-values can provide differenttissue contrast and signal to noise, thus, end user should befamiliar with NMR physics and/or use protocol specifically developedfor prostate MRI: Multiple different trace DWI images of twoprostate cancer lesions (right peripheral zone Gleason score 3+4 andleft peripheral zone 3+3 - whole mount prostatectomy section, partE). Trace DWI image in part C had the same b-value as image in partD, however, tissues contrast is different. The right peripheral zoneGleason score 3+4 lesion can be seen on part B, F and G while cannotbe seen in part C (due low signal-to-noise ratio), part D (due tolow prostate cancer to normal tissue contrast) and part H (due tolow prostate cancer to normal tissue contrast). The small leftperipheral zone Gleason score 3 + 3 lesion was not detected on anyDWI trace images.",yes
PMC7523209,Figure_3,oa_package/41/a0/PMC7523209.tar.gz,"[', 2020), JIB-04 was shown to exert a synergistic antiviral effect with chloroquine in MA104 cells (A).', 'A possible antiviral role for JIB-04 at a post-entry step was supported by the time of addition experiments (B).', 'A 1-h pre-treatment of cells with JIB-04 reduced SARS-CoV-2 spike mRNA transcription following VSV-SARS-CoV-2 infection (C), and translation of newly synthesized spike protein, which could not be achieved with Actinomycin D treatment (', 'When we compared the antiviral efficacy of these two JIB-04 isomers against VSV-SARS-CoV-2 in MA104 cells, the Z-isomer did not inhibit the replication of virus (E).', 'Knockdown of each gene successfully recapitulated the antiviral effect of JIB-04 (F and ', 'To identify potential target genes, we performed RNA-sequencing on the cells pre-treated with vehicle or JIB-04 with or without virus infection (G).', 'We validated by quantitative PCR that JIB-04 treatment reduced CYP1A1, CYP1B1, and AHRR mRNA levels by 4 6-fold (H).', 'Both compounds inhibited the replication of VSV-SARS-CoV-2 (I) and wild-type SARS-CoV-2 (', 'HSA synergy model was used to calculate the synergy scores of dose-response data in A.', '312165v2-f0002"" position=""float""/>.', 'A was performed twice.', 'Inhibition assay in J was performed once and cytotoxicity assay was performed in triplicates.']","Fig. 3. JIB-04 exhibits distinct post-entry antiviral mechanisms (A) Drug combination dose-response matrix and VSV-SARS-CoV-2 replication. MA104 cells were treated with JIB-04 and chloroquine for 1 h and infected with VSV-SARS-CoV-2 (MOI=3). GFP signals at 24 hpi were quantified to calculate the percentage of inhibition. (B) Time of compound addition and VSV-SARS-CoV-2 replication. MA104 cells were treated with JIB-04 (10 M) at indicated time points relative to VSV-SARS-CoV-2 infection (MOI=3, 0 hpi). GFP signals at 8 hpi were quantified to calculate the percentage of inhibition. (C) Intracellular SARS-CoV-2 S RNA levels with JIB-04 treatment. MA104 cells were treated with JIB-04 (10 M) for 1 h and infected with VSV-SARS-CoV-2 (MOI=1) for 1, 3, 5, and 7 h. S RNA levels were measured by RT-qPCR. (D) Western blot analysis of SARS-CoV-2 S protein levels with JIB-04 treatment. MA104 cells were treated with JIB-04 (10 M) for 1 h and infected with VSV-SARS-CoV-2 (MOI=1) for 1, 5, and 7 h. For Actinomycin D, 10 g/ml actinomycin D was added to the media 15 min before DMSO or JIB-04 treatment. (E) Intracellular viral RNA levels of cells treated with JIB-04 E-isomer or Z-isomer and subsequently infected with VSV-SARS-CoV-2. MA104 cells were treated with JIB-04 isomer (10 M) for 1 h and infected with VSV-SARS-CoV-2 (MOI=1). VSV-N levels at 24 hpi were measured by RT-qPCR. (F) Histone demethylase siRNA knockdown and RV replication. HEK293 cells were transfected with scrambled siRNA or siRNA targeting indicated histone demethylases for 48 h and infected with porcine RV (MOI=0.01). Viral RNA copy numbers at 12 hpi were quantified by RT-qPCR. (G) Volcano plot of differentially expressed transcripts with JIB-04 treatment and RV infection. HEK293 cells were treated with DMSO or JIB-04 (10 M) for 12 h, and mock-infected (left panel) or infected with porcine RV (MOI=0.01, right panel) for another 12 h. Red dots represent upregulated genes and green dots represent downregulated genes in JIB-04 treated cells. (H) Expression of three top genes in (G) with JIB-04 treatment. HEK293 cells were treated with JIB-04 (10 M) for 12 h and mock-infected or infected porcine RV (MOI=0.01) for 12 h. mRNA levels of , , at 12 hpi were measured by RT-qPCR. (I) Dose-response analysis of VSV-SARS-CoV-2 replication with fluoxetine or fluvoxamine treatment. MA104 cells were treated with compounds at 0.01 to 30 M for 1 h and infected with VSV-SARS-CoV-2 (MOI=3). GFP signals at 24 hpi were quantified to calculate the percentage of inhibition. For CC measurement, cells were treated with compounds at 0.1 M to 300 M for 25 h. (J) Dose-response analysis of wild-type SARS-CoV-2 replication with fluoxetine or fluvoxamine treatment. Vero E6 cells were treated with compounds for 1 h and infected with a clinical isolate of SARS-CoV-2 (MOI=0.5). S protein levels at 24 hpi were quantified based on immunofluorescence. For CC measurement, cells were treated with compounds at 0.1 M to 300 M for 25 h. For all panels except A and J, experiments were repeated at least three times with similar results. was performed twice. Inhibition assay in was performed once and cytotoxicity assay was performed in triplicates. Data are represented as mean SEM. Statistical significance is from pooled data of the multiple independent experiments (*p0.05; **p0.01; ***p0.001).",yes
PMC9796257,Figure_3,oa_package/f5/98/PMC9796257.tar.gz,[],"FIGURE 3 Case 58, a Spitz nevus/lowrisk (atypical) Spitz tumor from the right upper arm of an 11yearold male patient. NGS studies revealed a fusion. The image displays H&Estained sections at 100 magnification with a 400 inlay. Sections show a symmetric compound melanocytic neoplasm surmounted by epidermal hyperplasia. There are some small Kamino bodies in the epidermis. The junctional and dermal nests consist of monotonous appearing intermediatesized melanocytes with vesicular nuclei and scant cytoplasm. Hence, the scant cytoplasm makes this case difficult to immediately recognize as part of the Spitz family. Mitotic activity is readily identified (arrowhead) and maturation is somewhat impaired. Prior to genomic data, 43% of participants selected a Spitz diagnosis (answers D, E, F). After incorporating genomic data, 92% selected a Spitz diagnosis.",yes
PMC5500091,Figure_6,oa_package/7c/d5/PMC5500091.tar.gz,"['Photomicrograph (A C, HE, original magnification, 100; D, immunohistochemistry, 100) showed compact hyperplastic monocytes, foam cells (A, small arrow), and hemosiderin-laden macrophages (A, large arrow).']","Figure 6 Photomicrograph (AC, HE, original magnification, 100; D, immunohistochemistry, 100) showed compact hyperplastic monocytes, foam cells (A, small arrow), and hemosiderin-laden macrophages (A, large arrow). There were also a number of scattered multinucleated giant cells (B, arrows), and pale blue calcification (C, arrows) in the fibrous stroma. The monocytes and macrophages with a positive CD68 were showed in the immunohistochemistry (D).",yes
PMC7246086,Figure_4,oa_package/3a/6e/PMC7246086.tar.gz,"['4.', 'GGO: ground glass opacityPediatric COVID-19 CXR findings.']","Fig 4 Incidental COVID-19 findings in a 33-year-old female with abdominal pain, emesis, and fever. Axial CT abdomen/pelvis w/ IV contrast displays bilateral peripheral GGOs (arrows) ( ) and more consolidated peripheral opacities (arrows) ( ) as a peripheral finding. A diagnosis of COVID-19 was suggested on imaging with subsequent positive testing. The patient was taken to the operating room for abdominal findings under appropriate COVID-19 precautions. Coronal CT abdomen/pelvis w/ IV contrast displays ovarian torsion, with an enlarged ovary (long arrow) and twisting of adnexal vasculature (short arrow) ( ). GGO: ground glass opacity",yes
PMC6714522,Figure_16,oa_package/24/c5/PMC6714522.tar.gz,[],"Fig 16 Viewing from the posterior portal and looking anteriorly in the lateral decubitus position in a right (R) shoulder, the second limb of the suture is shuttled through the superior labrum, exiting superiorly approximately 1cm posterior to the first suture, and is pulled out through the anterior-superior portal.",yes
PMC4362789,Figure_1,oa_package/4f/f6/PMC4362789.tar.gz,"['Consistent with this observation, we observed a significant enhancement of the levels of secreted APP (sAPP ), the other product of -secretase processing of APP, in the conditioned medium (d and Supplementary S3c).', 'Co-expression of wild-type DISC1 (R1) rescued the changes elicited by DISC1 shRNAs, in particular those in the levels of APP-CTF , sAPP , A 42, and A 40 ().', 'In contrast to rescue by wild-type DISC1 (R1), mutant DISC1 lacking the APP binding domain (NR1) failed to normalize the biochemical changes in APP and A elicited by DISC1 knockdown ().', 'The role of amyloid beta-protein precursor and beta-amyloid in neurological disordersMol Psychiatry201318425434229258317BrandonNJSawaALinking neurodevelopmental and synaptic theories of mental illness through DISC1Nat Rev Neurosci201112707722220950648PorteousDJMillarJKBrandonNJSawaADISC1 at 10: connecting psychiatric genetics and neuroscienceTrends Mol Med201117699706220150219Young-PearseTLSuthSLuthESSawaASelkoeDJBiochemical and functional interaction of disrupted-in-schizophrenia 1 and amyloid precursor protein regulates neuronal migration during mammalian cortical developmentJ Neurosci20103010431104402068598510OddoSCaccamoAShepherdJDMurphyMPGoldeTEKayedRTriple-transgenic model of Alzheimer s disease with plaques and tangles: intracellular Abeta and synaptic dysfunctionNeuron2003394094211289541711HaraMRAgrawalNKimSFCascioMBFujimuroMOzekiYS-nitrosylated GAPDH initiates apoptotic cell death by nuclear translocation following Siah1 bindingNat Cell Biol200576656741595180712KamiyaAKuboKTomodaTTakakiMYounROzekiYA schizophrenia-associated mutation of DISC1 perturbs cerebral cortex developmentNat Cell Biol20057116711781629949813SeshadriSKamiyaAYokotaYPrikulisIKanoSHayashi-TakagiADisrupted-in-Schizophrenia-1 expression is regulated by beta-site amyloid precursor protein cleaving enzyme-1-neuregulin cascadeProc Natl Acad Sci U S A2010107562256272021212714LoisCHongEJPeaseSBrownEJBaltimoreDGermline transmission and tissue-specific expression of transgenes delivered by lentiviral vectorsScience20022958688721178660715IshizukaKChenJTayaSLiWMillarJKXuYEvidence that many of the DISC1 isoforms in C57BL/6J mice are also expressed in 129S6/SvEv miceMol Psychiatry2007128978991789592416KurodaKYamadaSTanakaMIizukaMYanoHMoriDBehavioral alterations associated with targeted disruption of exons 2 and 3 of the Disc1 gene in the mouseHum Mol Genet201120466646832190366817SchmidtSDJiangYNixonRAMathewsPMTissue processing prior to protein analysis and amyloid-beta quantitationMethods Mol Biol20052992672781598061118SchmidtSDNixonRAMathewsPMELISA method for measurement of amyloid-beta levelsMethods Mol Biol20052992792971598061219ChyungJHSelkoeDJInhibition of receptor-mediated endocytosis demonstrates generation of amyloid beta-protein at the cell surfaceJ Biol Chem200327851035510431452598920ParisiadouLEfthimiopoulosSExpression of mDab1 promotes the stability and processing of amyloid precursor protein and this effect is counteracted by X11alphaNeurobiol Aging2007283773881645839121HoeHSLeeKJCarneyRSLeeJMarkovaALeeJYInteraction of reelin with amyloid precursor protein promotes neurite outgrowthJ Neurosci200929745974731951591422JorissenEProxJBernreutherCWeberSSchwanbeckRSerneelsLThe disintegrin/metalloproteinase ADAM10 is essential for the establishment of the brain cortexJ Neurosci201030483348442037180323KuhnPHWangHDislichBColomboAZeitschelUEllwartJWADAM10 is the physiologically relevant, constitutive alpha-secretase of the amyloid precursor protein in primary neuronsEMBO J201029302030322067605624PostinaRSchroederADewachterIBohlJSchmittUKojroEA disintegrin-metalloproteinase prevents amyloid plaque formation and hippocampal defects in an Alzheimer disease mouse modelJ Clin Invest2004113145614641514624325WadsworthLPLoriusNDonovanNJLocascioJJRentzDMJohnsonKANeuropsychiatric symptoms and global functional impairment along the Alzheimer s continuumDement Geriatr Cogn Disord201234961112292282126RaudinoFNon-cognitive symptoms and related conditions in the Alzheimer s disease: a literature reviewNeurol Sci201327CorbettASmithJCreeseBBallardCTreatment of behavioral and psychological symptoms of Alzheimer s diseaseCurr Treat Options Neurol2012141131252232820428PoppJArltSPharmacological treatment of dementia and mild cognitive impairment due to Alzheimer s diseaseCurr Opin Psychiatry2011245565612193462129CannonTDHennahWvan ErpTGThompsonPMLonnqvistJHuttunenMAssociation of DISC1/TRAX haplotypes with schizophrenia, reduced prefrontal gray matter, and impaired short- and long-term memoryArch Gen Psychiatry200562120512131627580830HashimotoRNumakawaTOhnishiTKumamaruEYagasakiYIshimotoTImpact of the DISC1 Ser704Cys polymorphism on risk for major depression, brain morphology and ERK signalingHum Mol Genet200615302430331695979431ThomsonPAHarrisSEStarrJMWhalleyLJPorteousDJDearyIJAssociation between genotype at an exonic SNP in DISC1 and normal cognitive agingNeurosci Lett2005389414516054297APP processing is regulated by a protein-protein interaction with DISC1(a) Knockdown of DISC1 by lentiviral-mediated shRNA (D1) alters APP processing, compared to control shRNA (Con).']","Figure 1 APP processing is regulated by a protein-protein interaction with DISC1 Knockdown of DISC1 by lentiviral-mediated shRNA (D1) alters APP processing, compared to control shRNA (Con). This effect is rescued by co-expression of D1 and an shRNA-resistant wild-type DISC1 construct (R1), but not an shRNA-resistant mutant DISC1 construct lacking the binding domain for APP (NR1). Western blotting for full-length APP (APP-FL) and APP-CTF was done for each condition using lysates from mature primary cortical neuron cultures. Densitometric quantification of APP-FL expression in primary neurons expressing Con, D1, D1+R1, or D1+NR1. Densitometric quantification of APP-CTF expression in primary neurons expressing Con, D1, D1+R1, or D1+NR1. Analysis of conditioned media by ELISA shows increased sAPP and decreased A42 and A40 levels following DISC1 knockdown by D1. These effects are rescued by co-expression of R1, but not NR1. Bars represent the meanSEM for at least five independent experiments. ** <0.01, *** <0.001.",yes
PMC5817909,Figure_1,oa_package/81/b2/PMC5817909.tar.gz,"['TREM2 protein levels declined with Alzheimer s diseases progression in Down syndrome.', 'Supplementary MaterialSupplementary Activated astrocytes and microglia in Down syndrome brain.']","Fig.1 TREM2 protein levels declined with Alzheimers diseases progression in Down syndrome. To validate the TREM2 antibody (ab201621), the HEK293 cell line was transfected to overexpress human TREM2, cell lysates were analyzed by WB. The antibody recognized a band of 25 kDa and 18 kDa in the TREM2 overexpressing HEK293 cell lysate, whereas in control HEK293 cell lysate no band was seen (a). The WB analyses performed on postmortem human brain tissue (superior frontal cortex) showed that TREM2 levels were highest in controls, significantly decreased in DS and lowest in AD brains (b & c, <0.0005). TREM2 serum levels were significantly increased in young DS, lower in middle age, and lowest in the older subjects (e & f, <0.001 between older versus younger DS). Similarly, serum TREM2 levels were lower in AD ( =25), compared with age-matched controls ( =25) and lowest in DS (h & i, <0.001). -actin and human serum albumin were used as positive controls for brain tissue and serum samples, respectively (d, g, & j). Bonferroni-corrected Students -tests shown; ( =825 per group). Error bars indicate SEM. <0.05, <0.01, <0.001, one-way ANOVA.",yes
PMC3575255,Figure_3,oa_package/e1/a4/PMC3575255.tar.gz,"['The mature forms of cathepsin B and D decreased whereas Lamp1, Lamp2 and V0a1 proton pump subunit accumulated in PS1/APP mice hippocampus.']","Figure 3 ( - ) Western blots and quantitative analysis (n=5 for each age and animal group) of cathepsin B ( ) and D ( ) showed the existence of a significant decrease in the mature forms of both proteins. This decrease was observed exclusively in PS1/APP transgenic mice at both ages tested (6 and 18 months). - ) Same mice model accumulated Lamp1 (tested by western blot, C, n=3) or Lamp2 (tested by immunohistochemistry at 6 and 18 months of age). Lamp2 immunolabeled sections of 6 (d1) and 18 (d2) month-old PS1/APP mice counterstained with Congo red for fibrillar amyloid plaques revealed Lamp2 labeling associated to dystrophic neurites surrounding amyloid plaques (arrows) at both ages that increased with age. In WT mice (d3 and d4, for 6 and 18 months respectively) Lamp-2 immunostaining was associated to cell somata and neuropile. No dystrophies were detected in control samples. ) Representative western blot and quantitative analysis (n=5 for each age and animal group) of V0a1 proton pump subunit in samples from 6 and 18 month-old WT and PS1/APP mice. Both, the mature and immature forms of V0a1 seemed to be accumulated, although not significantly, in 6 and 18 months of age PS1/APP mice. CA1, hippocampal subfield; DG, dentate gyrus; so, stratum oriens; sp, stratum pyramidale; sr, stratum radiatum. Scale bars: 500m ( ), 25m (insets in d1 and d2).",yes
PMC5708188,Figure_5,oa_package/c1/5c/PMC5708188.tar.gz,"['Our results showed that there was a significant increase in Cu2+ content in the calluses of the 317L-Cu SS group compared with that of the calluses in the control group at 3, 6, and 9 weeks, which suggests that the increased Cu2+ content might be the basis for 317L-Cu SS to promote fracture healing and remodeling (A).', 'As expected, Runx2 expression in the 317L-Cu SS group was significantly higher than that in the 317L SS group at 3 and 6 weeks (B).', 'Therefore, 317L-Cu SS used for fracture fixation should not have systemic toxicity (C).', '317L-Cu stainless steel is conducive to the recovery of mechanical properties of the fractured femur.']","Figure 5 317L-Cu stainless steel significantly increased the Cu content in the callus and further enhanced the expression of Runx2 in the fracture local site. ( ) Significant increase in Cu content in the calluses of the 317L-Cu SS group compared with that of the control group at 3, 6, and 9 weeks. ( ) Runx2 expression in the 317L-Cu SS group is significantly higher than that in the 317L SS group at 3 and 6 weeks. ( ) 317L-Cu SS could not increase the concentration of serum Cu . * <0.05; ** <0.01. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; W, weeks.",yes
PMC10070117,Figure_3,oa_package/3b/39/PMC10070117.tar.gz,"['Then, we used TGF- 1 (10 ng/mL) to induce fibroblast differentiation to contractile myofibroblasts for 24 hours (Supplemental , A D).', 'As shown in A, THBS1-AS1 was about 72% distributed in the cytoplasm, while GAPDH was mainly expressed in the cytoplasm and U6 mainly in the nucleus.', 'Of interest, using RNA FISH, we observed that Cy3 (red fluorescence) was mainly located in the cytoplasm (B), suggesting that lncRNA THBS1-AS1 may mainly affect the expression level of genes by regulating the stability of mRNAs.', 'Immunofluorescence results showed that the knockdown of lncRNA THBS1-AS1 decreased the fluorescence intensity of -SMA (C).', 'Of note, the adenovirus-specific OE of lncRNA THBS1-AS1 promoted TGF- 1 induced activation of CFs and enhanced their proliferation, migration, and collagen contraction (, E and F, and Supplemental , B D).', 'The role of THBS1-AS1 on cardiac fibroblast activation.']","Figure 3 The role of THBS1-AS1 on cardiac fibroblast activation. ( ) LncRNA THBS1-AS1 is abundant in the cytoplasm of cardiac fibroblasts. GAPDH mRNA and U6 were applied as positive controls in the cytoplasm and nucleus, respectively. ( ) RNA FISH for THBS1-AS1 in cardiac fibroblasts. THBS1-AS1 was shown in red fluorescence (Cy3-labeled), and the nuclei were stained with DAPI. ( ) Immunouorescence images showing -SMA expression in cardiac fibroblasts. The images show staining for -SMA (green) and nuclei DAPI (blue) ( = 6). ( ) The EdU staining used to detect cell proliferation. The cell nuclei are stained blue (DAPI), and the EdU nuclei are stained red ( = 6). ( ) Immunouorescence images showing -SMA expression in cardiac fibroblasts ( = 6). ( ) The EdU staining used to detect cell proliferation ( = 6). One-way ANOVA, followed by a Bonferroni post hoc test, was used. The results are presented as means SEM; * < 0.05.",yes
PMC7683384,Figure_1,oa_package/ab/82/PMC7683384.tar.gz,"['Head CT showed right frontal lobe and insula ICH adjacent to the brain surface ().', 'Head CT showed right frontal lobe (arrows) and insula (arrowheads) intracerebral haemorrhage (ICH) adjacent to the brain surface.']","Figure 1 Head CT showed right frontal lobe (arrows) and insula (arrowheads) intracerebral haemorrhage (ICH) adjacent to the brain surface. Blood was also noted in the subarachnoid space, spreading from the basal cistern to the bilateral Sylvian fissure with hydrocephalus and brain herniation.",yes
PMC10621402,Figure_2,oa_package/cb/09/PMC10621402.tar.gz,['Endoscopy showing features of acid reflux.'],Figure 2 Endoscopy showing features of acid reflux. Arrow shows the esophagitis from the acid reflux.,yes
PMC5515959,Figure_4,oa_package/e8/34/PMC5515959.tar.gz,"[' 4b) compared with unmodified protein controls.', ' 4a,b).', '\nMicroscopy images of primary human monocyte adhesion to culture plates coated with fibronectin or tenascin C native proteins (PBS-only treated controls) or the deamidated forms of these ECM components (50 mM TEAB treated).', ' 4a,b).']",Figure 4 Microscopy images of differentiated U937 monocytic cell adhesion to culture plates coated with ECM proteins tenascin C (TNC) or deamidated tenascin C (dTNC) in different experimental conditions ( ). Scale bar indicates 100m. Quantitation of cell adhesion to ECM proteins Tenascin C and deamidated Tenascin C under different experimental conditions. Statistics were calculated from experimental triplicates. Tenascin C (TNC); Deamidated Tenascin C (dTNC). Differentiated U937 cells (Cont) were treated or not with Cilengitide (cgt) or monoclonal antibodies against integrin (In ) or integrin (In ).,yes
PMC8896839,Figure_2,oa_package/ac/35/PMC8896839.tar.gz,['MGMT status in glioblastoma.'],"Figure 2 MGMT status in glioblastoma. (A) MGMT positive tumor, IHC marking for MGMT, original magnification 200; (B) MGMT positive tumor, IHC marking for MGMT, original magnification 400. MGMT: O6methylguanineDNA methyltransferase; IHC: immunohistochemistry.",yes
PMC3664970,Figure_1,oa_package/8f/45/PMC3664970.tar.gz,"['Hemostats can be used at this point to hold the muscle layer apart for easy access to the thyroid gland ().', 'jpg"" position=""float"" orientation=""portrait""/>\n .']",,yes
PMC3992335,Figure_2,oa_package/8e/72/PMC3992335.tar.gz,"[""Masson's trichrome stain high-lighted centrilobular venular fibrosis and perisinusoidal fibrosis, and fibrous occlusion of small terminal hepatic venules was seen ()."", 'Representative microscopic findings of sinusoidal obstruction syndrome (A-D).']","Figure 2 Representative microscopic findings of sinusoidal obstruction syndrome (A-D). Patchy perivenular sinusoidal dilatation and congestion with hepatocyte plate disruption (A 100 magnification). Approximation of portal structures and hepatic vein with loss of intervening hepatocytes, and perivenular and perisinusoidal fibrosis (B 100 magnification, Masson's trichrome stain). Sinusoidal dilatation and congestion with atrophy and disruption of hepatocyte plates (C 200 magnification). An obliterated terminal hepatic venule is seen in the center (arrow). Masson's trichrome stain demonstrating fibrous obliteration of a terminal hepatic venule (D 400 magnification).",yes
PMC5036232,Figure_6,oa_package/65/b5/PMC5036232.tar.gz,"['We found that LT R was widely expressed by all four cholangiocarcinoma cell lines (Oz, KMBC, HuCCT1 and Mz-ChA-1) we tested, as well as by two HCC cell lines Huh1 and HLE (figure 6A).', 'Knockdown of LT R in Huh1 and Oz cells with targeting siRNA resulted in decreased protein expression/activation of pAKTser473, CAT, NICD and Hes1 levels at 48 h post transfection (figure 6B), suggesting that LT R signalling may be important for maintaining the activity of these oncogenes in human liver cancer cells.', '5081) (figure 6C).', 'Moreover, ingenuity pathway analysis of differentially expressed genes of ICC vs normal were enriched in Notch, phosphatase and tensin homolog (PTEN) and PI3K/AKT signalling pathways and associated with high LTBR gene expression (figure 6C, right panel).', 'In addition, hierarchal clustering of proliferative class genes revealed a subset of significantly regulated proliferative genes similarly clustering with LTBR, NOTCH1 and Hes1 (figure 6D).', 'Furthermore, an ICC cohort of Thai patients obtained for study through the TIGER-LC consortium (Chaisaingmongkol et al, manuscript in preparation) stratified LT R high (n=43) expression with significantly worse survival as compared with ICC cases with LT R low (n=42) expression (figure 6E).']","Figure6 LTR signalling regulates oncogene activities in human liver cancers and correlates with poor survival of patients with intrahepatic cholangiocarcinoma (ICC). (A) Flow cytometry analysis of LTR surface expression in cholangiocarcinoma (Oz, HuCCT1, KMBC, MZ-ChA-1) and HCC (Huh1, HLE, HepG2, Huh7) cell lines. (B) siRNA specific for LTR (si-L) was transfected into Huh1 and Oz cells. 48h later, LTR shutdown efficiency was confirmed by RT-PCR (top two panels), and Notch1/Hes1/AKT/pAKT /CAT protein level were analysed by western blot (WB) with cell lysates (bottom panels) compared with control siRNA (si-C) (C) Reanalysis of Lovelt ICC cohort, LTR expression relative correlation with LT (left panel) and Notch1(centre panel) expression. Further, IPA of differentially expressed genes of ICC versus normal was performed to detect signalling pathways associated with high gene expression (right panel). p Values on the side of the bars are from Fisher's exact test using the IPA database, X-axis islog pvalue of IPA calculated p value. (D) LTR, Hes1 and Notch1 heat map was constructed from statistically significant proliferative class genes between Lovelt cohort ICC versus normal biliary epithelial cells (494 genes), normalised log2 transformed and hierarchal clustering performed (Pearson's correlation-complete linkage). (E) Patient survival data from TIGER-LC Thailand cohort was stratified based on LTR high (n=43) and low (n=42) expressions. CAT, catenin; HCC, hepatocellular carcinoma; IPA, ingenuity pathway analysis.",yes
PMC6514201,Figure_4,oa_package/2e/b4/PMC6514201.tar.gz,"[' 4a c).', ' 4d f).', ' 4g) and the filamentous structure of the neurofibrillary tangles (', ' 4h).', ' 4i).', '', 'Bar (in i) represents 50 m in a, c, d, f, g, and i; 20 m in b, e, and hN-224 tau and t-tau concentrations show a decreasing trend in AD brain samples along Braak stagesThere was no significant difference in the concentration between AD and controls for the N-123 fragment (p = 0.', ' 4a, b), suggesting that this fragment is deposited in AD and colocalized with the tangles.', ' 4d f).']","Fig.4 Tau immunohistochemistry in Alzheimers disease ( and ; and ; and ) and a neurologically normal control ( , , and ). Anti-tau 224 immunohistochemistry shows neurofibrillary tangles ( , arrow) and dystrophic neurites ( , double arrow) surrounded by neuropil threads. Neurofibrillary tangles show at higher magnification ( ). Faint punctuate neuronal cytoplasmic staining was observed in the normal control. Neurons in AD are weakly positive for anti-tau 123 and negative in the normal control. For comparison, sequential sections were immunohistochemically stained with AT8 to show the presence of neurofibrillary tangles in AD ( , arrow), dystrophic neurites ( , double arrow), and filamentous structure of the neurofibrillary tangles ( ). AT8 staining is absent in the normal control. Bar (in ) represents 50m in , , , , , and ; 20m in , , and",yes
PMC6023668,Figure_3,oa_package/5c/43/PMC6023668.tar.gz,['Gross appearance of the anterior mediastinal tumor.'],Figure 3 Gross appearance of the anterior mediastinal tumor. The anterior mediastinal tumor measured 6525mm and showed a multinodular lesion with fibrosis.,yes
PMC10515780,Figure_1,oa_package/13/6a/PMC10515780.tar.gz,"['The methodology is illustrated in A.', 'We further performed lipidomic studies on a subset of these donors to validate pathologies described below, and single nucleus RNAseq (snRNAseq) to define pathology in glial cells (see below) A.', 'A general inspection of the dataset in the reduced dimensional space (tSNE) is provided in B which shows samples color-coded by condition and anatomic region, and by sex and sequencing batch in S1A.', 'Overall, HD samples were enriched in the upper half of the tSNE projections (B).', 'There were numerous differentially expressed genes (DEGs), and we show a subset of these genes in C.', 'The gene expression heatmap demonstrates upregulation of several genes involved in control of gliogenesis/stemness such as NES, EGFR, GLI1, PTCH1, YAP1, POU4F2, SMAD4, and REST, and downregulation of several genes involved in oxidative phosphorylation and mitochondrial function, as well as several known HD medium spiny neuronal genes such as dopamine receptor genes DRD1, DRD2, striatal identity gene PCP4 (C and supplementary table-2).', 'Next, we asked to what extent were the DEGs shared between brain regions (D and Supplementary Table-2).', 'The overlap between DEGs among brain regions was most notable between the accumbens and caudate on the one hand both striatal regions, and accumbens and cingulate on the other hand both less severely involved in HD (D).', 'The results identified 1092 genes with either positive (672) or negative (420) regression weights (E and Supplementary Table-2, we refer to these genes as CAG-correlated ).', 'Next, we performed KEGG pathway enrichment analysis of the CAG-correlated genes and DEGs shared between two or three regions (F G and Supplementary Table-2).', 'As expected, CAG-correlated genes were enriched in pathways related to splicing, protein processing, autophagy, neurodegeneration, and lysosomal function (F).', 'Interestingly, in both gene sets, KEGG pathways involved in inflammation, cancer-related pathways, and lipid metabolism were significantly enriched (F G).', 'Below, we present the fibrous-like and protoplasmic astrocytic clusters annotated by anatomic locale, clusters markers, and enrichment of specific informative gene signatures that we are interested: 1) a putative neuroprotective gene signature which we defined based on our previous work to be enriched in metallothionein genes51 encoding proteins rich in cysteine amino acids known to confer protection against oxidative damage52, 2) CAG-correlated genes (see ), 3) genes associated with quiescent astrocytes51 which are indicators of baseline astrocyte function including regulation of glutamate transport and glycolysis, and 4) genes correlated with the HD lipidomic signature which are associated with unfolded protein response (see ).', '36303071.', 'A) Dot plot displaying the expression of various genes from four different gene sets (Quiescent Genes = baseline astrocyte genes51, Neuroprotective Genes as predicted from our previous work51, CAG-correlated genes = genes with significant positive regression weights - see E for more details, RNA-correlated Lipid Genes = set of genes that correlated with lipid abundance from S3D.']","Figure 1. Transcriptomic analysis of HD identifies cross-regional and CAG-correlated gene signatures. ) Cartoon depicting experimental plan. ) t-distributed stochastic neighbor (tSNE) embedding of bulk RNAseq samples used in the study color-coded by condition (left), anatomic region (middle), and CAG repeat length (right). Control samples and ones with no available CAG repeat lengths are shown in grey. ) Heatmap of normalized gene expression showing a select subset of DEGs. The differentially expressed genes (DEGs - rows) are color-coded on the right by the direction of differential expression (Control -red vs HD blue vs non significant (NS) grey), and anatomic region where comparisons are significant (Sign_Acc: Accumbens, Sign_Caud: Caudate, Sign_Cing: Cingulate). The samples (Columns) are also color-coded by HD grade/Condition (Con: Control, HD14: 14, J: Juvenile onset HD). ) Venn diagram showing the overlap between DEGs increased black; and decreased blue across the anatomic regions indicated. The genes consider for this analysis were considered significant if the adjusted p value was less than 0.05. ) Scatter plot showing genes with significant correlation with CAG repeat length. The correlation coefficient is shown on the y-axis, the order of genes on the x-axis is random. Color indicates adjusted p value. Genes with coefficients two standard deviations above the mean are indicated. ) EnrichR bar plots of KEGG pathways enriched in genes that positively or negatively correlate with CAG repeat length ( ) or DEGs shared that are shared across two or three anatomic regions (increased and decreased - ).",yes
PMC4540825,Figure_1,oa_package/bf/80/PMC4540825.tar.gz,"['The American College of Radiology, Breast Imaging Reporting And Data System (ACR BI-RADS,\nUSA) categorizes breast parenchymal pattern density from mammography into four grades: grade\n1, almost entire fat; grade 2, fibroglandular densities; grade 3, heterogeneously dense; and\ngrade 4, extremely dense (']","Fig. 1. Mammography. a) represents almost entirely fat, b) represents fibroglandulardensities, c) represents heterogeneously dense, and d) represents extremely dense",yes
PMC8947699,Figure_2,oa_package/ab/6e/PMC8947699.tar.gz,"['Histological examination revealed a specimen composed of non-dysplastic parakeratinized oral mucosa supporting a markedly infiltrative, non-encapsulated malignant neoplastic odontogenic epithelial proliferation with a prominent clear cell component, supported by dense fibrous connective tissue stroma (A).', 'The neoplastic cells were arranged in anastomosing trabeculae and exhibited nuclear hyperchromasia and pleomorphism surrounded by optically clear, vacuolated cytoplasm (B,C).', 'Occasional atypical mitotic figures were observed (D).', 'Non-dysplastic parakeratinized surface epithelium with a subepithelial markedly infiltrative, non-encapsulated malignant neoplastic odontogenic epithelial proliferation with a prominent clear cell component (A, 40 ).']","Figure 2 Non-dysplastic parakeratinized surface epithelium with a subepithelial markedly infiltrative, non-encapsulated malignant neoplastic odontogenic epithelial proliferation with a prominent clear cell component ( , 40). Neoplastic cells were arranged in anastomosing trabeculae and nests exhibiting nuclear hyperchromasia and pleomorphism and optically clear cytoplasm ( , , 100 & 200, respectively). Nuclear pleomorphism and cytoplasmic clearing ( , 400).",yes
PMC8617390,Figure_1,oa_package/5a/41/PMC8617390.tar.gz,"['Epiaortic echocardiographic view reassured the graft patency at the arch [].', 'Epiaortic echocardiography using Multiplane and Color Flow Doppler.']",Figure 1 Epiaortic echocardiography using Multiplane and Color Flow Doppler. Demonstrates systolic flow in the graft at the arch level and presence of color artifact in the suture line and wall,yes
PMC10667884,Figure_3,oa_package/1d/20/PMC10667884.tar.gz,[],"Image 3 Coronal CT of the abdomen and pelvis showing distended hepatic flexure with wall thickening (yellow arrow), distended ascending colon with wall thickening (blue arrow), focal narrowing and thickening of the sigmoid colon (red arrow) with distended small bowel (green arrow).",yes
PMC6637505,Figure_5,oa_package/11/87/PMC6637505.tar.gz,"[' 5a).', '5a).', '5a).', '5b).', '5c).', '5d).', '5e).', '5f).', 'A strong inflammatory response was observed in the optic nerve and retina of EAE mice.', 'EAE: experimental autoimmune encephalomyelitis, IBA1: allograft inflammatory factor 1, TMEM119: transmembrane protein 119, IRL: inner retinal layer, INL: inner nuclear layer, ONL: outer nuclear layerDemyelination and axonal pathologyInitial signs of demyelination (loss of MBP), a key factor implicated in MS and EAE pathology, were observed in the optic nerve of EAE mice at 11 dpi progressing until 33 dpi, at which the greatest loss of immunoreactivity was detected (', '5), likely further amplified the inflammatory process within the retina, potentially contributing to the acute IRL thickening.', '5e) and decreased expression of Bdnf (', '5b).', '5c), can contribute to synaptic deficits in addition to inducing neuronal cell death by controlling the release of glutamate from astrocytes [66 69].', '5d), that may be released by both astrocytes and microglia, is involved in mediating immunopathology and likely has an amplifying, rather than an initiating role [67, 70 72], promoting further retinal damage.']","Fig. 5 A strong inflammatory response was observed in the optic nerve and retina of EAE mice. T-cells (CD3) initially appear 11days post immunisation (dpi) along with the cellular infiltration (DAPI), subsiding at later time points but still remaining until 33 dpi in the optic nerve of EAE mice. T-cells were not observed in the retina of EAE mice at any time point. Yet, expression amplified from 20 to 33 dpi in EAE compared to healthy controls in the retina. is a proinflammatory cytokine involved in the innate immune response. Whereas, mRNA expression of in the retina increased significantly between 15 dpi and 28 dpi in EAE mice. likely plays an amplifying role (rather than an initiating role) in EAE. Caspase 1 ( ) increases significantly from 15 dpi to 33 dpi in EAE retinas. It has a crucial role in development of immune mediated inflammatory processes leading to central nervous system demyelination. However, , which is a cytokine activated by Caspase 1, was not different in retinal expression between EAE mice and healthy controls. -values for comparison between EAE and control mice (**** <0.00001, *** <0.0001, ** <0.01). EAE: experimental autoimmune encephalomyelitis, TNF: tumor necrosis factor, MCP1: monocyte chemoattractant protein 1, IL-1: interleukin-1, IRL: inner retinal layer, INL: inner nuclear layer, ONL: outer nuclear layer. EAE: experimental autoimmune encephalomyelitis, IBA1: allograft inflammatory factor 1, TMEM119: transmembrane protein 119, IRL: inner retinal layer, INL: inner nuclear layer, ONL: outer nuclear layer",yes
PMC11254429,Figure_2,oa_package/72/88/PMC11254429.tar.gz,"['6\nThe anterior component was detached, distally, at the level of the anterior tibial tuberosity, and the posterior component, proximally, at the level of the patella\n6\n(\n\n).', '6\n\nScheme of the surgical procedure: patellar ligament was divided in two, according to its thickness.', '6\nO componente anterior foi desinserido, distalmente, ao n vel da tuberosidade anterior da t bia e o componente posterior, proximalmente, ao n vel da patela\n6\n(\n\n).', '6\n\nEsquema do procedimento cir rgico: ligamento patelar foi dividido em dois, segundo a sua espessura.']","Fig. 2 Scheme of the surgical procedure: patellar ligament was divided in two, according to its thickness. The anterior bundle (dark blue) was detached anteriorly at the level of the anterior tibial tuberosity, the posterior bundle (light blue) was detached at the level of the distal apex of the patella. Also note the acquisition of a bundle of the quadriceps tendon (gray). (1Quadriceps Tendon; 2Patella; 3Patellar Ligament).",yes
PMC3510423,Figure_1,oa_package/c5/bd/PMC3510423.tar.gz,"[' 1a).', ' 1b).', '', 'PG almost healed after 64 weeks of treatment with adalimumab (b)\n']",Fig.1 Large pyoderma gangrenosum (PG) defect on the right lower leg after failure of several standard therapies and before treatment with adalimumab ( ). PG almost healed after 64weeks of treatment with adalimumab ( ),yes
PMC2965905,Figure_1,oa_package/13/41/PMC2965905.tar.gz,"['[11011] A case of oral lichen planus was also included in our study [].', '[6714]Oral lichen planus - Shows plaque like, violaceous lesions over the buccal and glossal mucosaPsoriasis - Shows erythematous and scaly lesions of the palmsAs far as histology is concerned, all our cases of lichen planus showed hyperkeratosis, hypergranulosis, sometimes focally, irregular acanthosis, vacuolar degeneration of the basal layer and a band-like infiltrate in the papillary dermis [].']","Figure 1 Oral lichen planus - Shows plaque like, violaceous lesions over the buccal and glossal mucosa",yes
PMC3306430,Figure_3,oa_package/bc/07/PMC3306430.tar.gz,"['Hair follicles stain weakly for FN14, whereas sebaceous glands and especially sweat glands stain strongly for FN14 and TWEAK (A).', 'g003FN14 and TWEAK are detected in normal and pathological skin.', 'In basal cell carcinomas TWEAK and Fn14 are highly expressed only in palisading cells (B).', 'In contrast, TWEAK and FN14 are highly expressed in squamous cell carcinoma, a tumor that is characterized by an intense inflammatory component (B).', 'In psoriasis (C), a benign skin lesion that is characterized by intense inflammation, the keratinocytes heavily express TWEAK and FN14.']",10.1371/journal.pone.0033609.g003,yes
PMC8525644,Figure_9,oa_package/41/0d/PMC8525644.tar.gz,"['In WT mice, a transient increase in SMA was observed at 2 days after caerulein dosing, and this increase gradually subsided to basal levels in 7 days (, A and B).', 'At 6 hours after caerulein, there was a 2- and 4-fold increase in TGF 1 levels in WT and KO mice, respectively (C).', 'However, TGF 1 went back to basal level in WT mice and continued to remain higher in the KO group for up to 7 days after final caerulein injection (C).', 'Although the miR-29a/b1 KO mice exhibited increased activity of phospho-JNK at 6 hours, the activity was lower than that in the WT group (D).', 'In addition, examination of the activation status of c-Jun, a downstream effector of the JNK pathway, also revealed similar patterns, where an increased activity of c-Jun was observed in both the groups at 6 hours, although the activity in the KO group was significantly lower compared with the WT mice at this time point (D).', 'Loss of miR-29a induces enhanced activation of pancreatic stellate cells (PSCs) in AP mice.']","Figure 9 Loss of miR-29a induces enhanced activation of pancreatic stellate cells (PSCs) in AP mice. ( and ) Representative IHC images for SMA staining in pancreatic sections of WT and miR-29a/b1KO mice treated with saline (Cer) or at 6 hours, 2 days, 4 days, 7 days, and 10 days after caerulein injections. Scale bars: 200 m. SMA area was quantified per 5 high-powered fields. IHC images represent = 5 animals/group/time point. ( ) TGF1 levels were measured in pancreatic homogenates by ELISA for WT and miR-29a/b1KO mice treated with saline (Cer) or caerulein at indicated time points ( = 3 animals/group/time point). ( ) Western blot analysis from pancreatic homogenate for phosphop46 JNK, and phosphoc-Jun. GAPDH was used as the loading control. Western blot images are representative of 3 mice/group/time point. Results in graph represent mean SEM. Asterisk denotes difference with saline-treated WT mice; # symbol denotes difference between caerulein dosed WT and miR-29aKO mice at a given time point; * < 0.05, < 0.05, ** < 0.01, or < 0.01, 2-way ANOVA with Bonferroni post hoc test.",yes
PMC9306679,Figure_2,oa_package/6c/37/PMC9306679.tar.gz,['H E stain showing disorganized and bland spindle cells associated with abundant eosinophilic cytoplasmH E: Hematoxylin and eosin.'],Figure 2 H&E stain showing disorganized and bland spindle cells associated with abundant eosinophilic cytoplasm H&E:Hematoxylin and eosin.,yes
PMC9302320,Figure_1,oa_package/44/6f/PMC9302320.tar.gz,['.'],"Figure 1. Clinical features of nondystrophic myotonia. Left (AD): A patient with MC showing muscle stiffness, muscular hypertrophy and grip myotonia. Right (EG): A patient with PMC showing muscle stiffness, joint contracture and muscular hypertrophy with lower limbs affected.",yes
PMC9157357,Figure_3,oa_package/0c/9e/PMC9157357.tar.gz,"['Skin flaps were elevated using diathermy (figure 3).', 'Depicting bilateral cheek flap elevation and exposure of desired segments of zygoma and maxilla.']",Figure 3 Depicting bilateral cheek flap elevation and exposure of desired segments of zygoma and maxilla.,yes
PMC10730909,Figure_7,oa_package/ed/e3/PMC10730909.tar.gz,"[' 7a, b).', ' 7c).', ' 7d).', ' 7e), which cooperates with p62 in forming local clusters on protein aggregates16.', ' 7f, g).', 'p62 binds to aSyn aggregates in a NEMO-dependent manner.', 'NEMO promotes p62 condensate formation at aggregates by lowering the threshold for ubiquitin-induced phase transitionWe and others recently observed that NEMO undergoes phase separation upon binding to linear ubiquitin chains50,79.']","Fig. 7 p62 binds to aSyn aggregates in a NEMO-dependent manner. Colocalization of p62 and aSyn aggregates is decreased in the Q330X NEMO patient brain despite increased p62 expression. Paraffin-embedded brain sections from control, DLBL (Dementia with Lewy Bodies) or the Q330X NEMO patient brain were analyzed by immunohistochemistry and fluorescence SR-SIM using antibodies against aSyn and p62. Scale bar, 200m. Foci staining positive for both aSyn and p62 were quantified in 28-30 fields of view per brain section. Data are shown as meanSEM, =28/30 individual cells. Foci staining positive for p62 only were quantified in 10 fields of view per brain section. Data are displayed as meanSEM from 10 fields of view. Statistics: KruskalWallis test followed by Tuckeys multiple comparison test. *** 0.001. Foci-like concentration of p62 and NBR1 at aSyn aggregates is reduced in NEMO-deficient cells. CRISPR/Cas9 NEMO KO or wildtype (WT) SH-SY5Y cells were transiently transfected with aSyn A53T-GFP. One day after transfection, the cells were treated with aSyn A53T seeds, fixed 48h after seeding, and analyzed by immunohistochemistry and fluorescence SR-SIM using antibodies against p62 and NBR1 . 3D-reconstructions were performed using the surface module of Imaris 10.0.1 image analysis software. Scale bars, D/E: 1m. The abundance of LC3 and LAMP2 at aSyn aggregates is decreased in NEMO-deficient cells. CRISPR/Cas9 NEMO KO or WT SH-SY5Y cells were transiently transfected with aSyn A53T-GFP. One day after transfection, the cells were treated with aSyn A53T seeds, fixed 48h after seeding, and analyzed by immunohistochemistry and fluorescence SR-SIM using antibodies against LC3 and LAMP2 . 3D-reconstructions were performed using the surface module of Imaris 10.0.1 image analysis software. Coverage of aSyn-GFP aggregates by LC3 and LAMP2 was analyzed using the Imaris 10.0.1 surface modules and a surface-to-surface MatLab Plugin. The coverage of the reconstructed aSyn-GFP surface was quantified and plotted as percentage of the total aSyn-GFP aggregate surface for LC3 and LAMP2 . Data are displayed as meanSEM, =11/20 individual cells for LC3 (* =0.0422) and =14/13 individual cells for LAMP2 (* =0.0143). Statistics: two-tailed students -test. * 0.05. Scale bars, 2m (left panel), 1m (right panel), 0.5m (left panel), 1m (right panel).",yes
PMC6607698,Figure_2,oa_package/f5/87/PMC6607698.tar.gz,"['Then, a formal lateral neck dissection released the medial investing fascia of the sternocleidomastoid muscle enabling its further lateral retraction ().', '001""/>View of lipoma after initial dissection with release of the left investing layer (posterior fascia of the sternocleidomastoid muscle) of the deep cervical fascia.']",Figure 2 View of lipoma after initial dissection with release of the left investing layer (posterior fascia of the sternocleidomastoid muscle) of the deep cervical fascia.,yes
PMC9899715,Figure_5,oa_package/5c/91/PMC9899715.tar.gz,"[' 5 shows the case of patient 10, who presented the 4th recurrence of inverted papilloma in the frontorbital groove and had previously undergone a Draf III.', 'C Endoscopic view with the Narrow-Band Imaging enhancing the vascular patternWe had no immediate and short-term complications related to the use of the FIE with a median follow-up time of 6 months (IQR: 4, range 2 15).']",Fig. 5 Illustration of case number 10. MRI: coronal view showing the recurrence of the inverted papilloma on the lateral recess of the right frontal sinus. Endoscopic view with the white light. Endoscopic view with the Narrow-Band Imaging enhancing the vascular pattern,yes
PMC7716756,Figure_2,oa_package/db/d3/PMC7716756.tar.gz,[],Figure 2 Nodular tumors on the nasal pyramid and on the skin part of the upper lip,yes
PMC7483032,Figure_1,oa_package/cb/5b/PMC7483032.tar.gz,"['Gastric foveolar epithelium appears as large irregular folded or monolayered sheets of mucin-containing columnar cells (A).', 'Duodenal epithelium is also characterized by sheets of columnar cells with interspersed goblet cells, resulting in a characteristic starry-sky appearance (B).', 'The presence of scattered goblet cells within monotonous-looking cell nests is a helpful clue that can differentiate normal intestinal mucosa from IPMNs (B).', 'A meta-analysisJ Dig Dis201617951032671374913WeynandBBorbathIBernardVPancreatic neuroendocrine tumour grading on endoscopic ultrasound-guided fine needle aspiration: high reproducibility and inter-observer agreement of the Ki-67 labelling indexCytopathology201425389952475027214HwangHSKimYAnSGrading by the Ki-67 labeling index of endoscopic ultrasound-guided fine needle aspiration biopsy specimens of pancreatic neuroendocrine tumors can be underestimatedPancreas20184712963033021180515KimSAKimMSKimMSPleomorphic solid pseudopapillary neoplasm of the pancreas: degenerative change rather than high-grade malignant potentialHum Pathol201445166742432152616KimSBaeHChoiMIsolated mass-forming IgG4-related cholangitis as an initial clinical presentation of systemic IgG4-related diseaseJ Pathol Transl Med20165030052675536017LeeHEZhangLImmunoglobulin G4-related hepatobiliary diseaseSemin Diagn Pathol201936423333135842518HeymannJJSiddiquiMTAncillary techniques in cytologic specimens obtained from solid lesions of the pancreas: a reviewActa Cytol202064103233097035019LayfieldLJEhyaHFilieACUtilization of ancillary studies in the cytologic diagnosis of biliary and pancreatic lesions: the Papanicolaou Society of Cytopathology guidelines for pancreatobiliary cytologyDiagn Cytopathol201442351622463939820SoyerOMBaranBOrmeciACRole of biochemistry and cytological analysis of cyst fluid for the differential diagnosis of pancreatic cysts: a retrospective cohort studyMedicine (Baltimore)201796e55132807269221KubiliunNRibeiroAFanYSEUS-FNA with rescue fluorescence in situ hybridization for the diagnosis of pancreatic carcinoma in patients with inconclusive on-site cytopathology resultsGastrointest Endosc20117454172175236422WuJMatthaeiHMaitraARecurrent GNAS mutations define an unexpected pathway for pancreatic cyst developmentSci Transl Med2011392ra6623JonesMZhengZWangJImpact of next-generation sequencing on the clinical diagnosis of pancreatic cystsGastrointest Endosc20168314082625301624NgamruengphongSLennonAMAnalysis of pancreatic cyst fluidSurg Pathol Clin20169677842792636625RosenbaumMWJonesMDudleyJCLeLPIafrateAJPitmanMBNext-generation sequencing adds value to the preoperative diagnosis of pancreatic cystsCancer Cytopathol20171254172764780226HartleyCPMahajanAMSelvaggiSMRehrauerWMFNA smears of pancreatic ductal adenocarcinoma are superior to formalin-fixed paraffin-embedded tissue as a source of DNA: Comparison of targeted KRAS amplification and genotyping in matched preresection and postresection samplesCancer Cytopathol2017125838472902453027ParkYJKimGHParkDYHistopathologic discrepancies between endoscopic forceps biopsy and endoscopic resection specimens in superficial esophageal squamous neoplasmsJ Gastroenterol Hepatol2019341058653055271728IlieMLong-MiraEBenceCComparative study of the PDL1 status between surgically resected specimens and matched biopsies of NSCLC patients reveal major discordances: a potential issue for anti-PD-L1 therapeutic strategiesAnn Oncol201627147532648304529CrosJRaffenneJCouvelardAPoteNTumor heterogeneity in pancreatic adenocarcinomaPathobiology20188564712878774130PrasetyantiPRMedemaJPIntra-tumor heterogeneity from a cancer stem cell perspectiveMol Cancer201716412820916631AllensonKCastilloJSan LucasFAHigh prevalence of mutant KRAS in circulating exosome-derived DNA from early-stage pancreatic cancer patientsAnn Oncol20172874172810462132TakaiETotokiYNakamuraHKatoMShibataTYachidaSClinical utility of circulating tumor DNA for molecular assessment and precision medicine in pancreatic cancerAdv Exp Med Biol20169241372775301133TorresCGrippoPJPancreatic cancer subtypes: a roadmap for precision medicineAnn Med201850277872953730934ChantrillLANagrialAMWatsonCPrecision medicine for advanced pancreas cancer: the Individualized Molecular Pancreatic Cancer Therapy (IMPaCT) trialClin Cancer Res20152120293725896973.']",Fig. 1. (A). Normal gastric mucosa in conventional cytology smear shows nests of monotonous cells with smooth boundaries. (B) Normal duodenal mucosa showing scattered goblet cells in conventional cytology smear (Pap smear).,yes
PMC8544688,Figure_3,oa_package/e5/c4/PMC8544688.tar.gz,"['Mice treated with limbal fibroblast-conditioned media revealed considerable growth of corneal-type epithelial cells shown by expression of K12 on the corneal surface shown ((a1)) and less conjunctival goblet cells shown by fewer expression of K8 ((a2)).', 'However, the LSCD corneas treated with DMEM and skin fibroblast condition media were found to be covered primarily by conjunctival type epithelium, low expression of K12 ((b1,c1)) and high expression of goblet K8 cells ((b2,c2)).', 'Representative immune staining of whole-mount corneas from mouse model of LSCD that are treated with three conditioned media.']","Figure 3 Representative immune staining of whole-mount corneas from mouse model of LSCD that are treated with three conditioned media. Corneas treated with human limbal fibroblast-conditioned media showed consistent expression of K12 (red, ( )) and lower expression of K8 (green, ( )) which shows the therapeutic effect of this media. However, corneas treated with DMEM or human skin-conditioned media as a negative control illustrated low expression of K12 (( , ) respectively) and high expression of K8 (green, ( , ) respectively). Mouse model of LSCD treated with conditioned media. This study created a mouse model of LSCD through limbus to limbus scraping. Mice were then treated with three weeks of limbal fibroblast-conditioned media (AF), DMEM (GI) or skin-conditioned media (JL). K8-positive cells fluoresce green, while K12 positive cells fluoresce red. M and N are magnified and stained with DAPI [ ].",yes
PMC8489661,Figure_4,oa_package/28/7b/PMC8489661.tar.gz,[],"Figure4 Soluble Worm Antigens (SWA) increases the secretion of LCN2 in macrophages RAW264.7 cells. The secretion of LCN2 by RAW264.7 increased in a concentration-dependent manner. LCN2 increased most significantly at 24 h after SWA stimulation. After 24 h of SWA treatment, compared with the control group, M1 polarization increased significantly, while M2 polarization decreased (*p < 0.05, **p < 0.01, ***p < 0.001).",yes
PMC9410577,Figure_8,oa_package/04/e9/PMC9410577.tar.gz,['.'],"Figure 8. (AE) The ROC curve of targets of curcumin against AD-related tau and a pathology. AD = Alzheimer disease, ROC = receiver operating characteristic.",yes
PMC10349115,Figure_5,oa_package/e3/92/PMC10349115.tar.gz,"[' 5a,b).', ' 5a,b) suggested that the early activation of the complement system was instrumental for inflammatory cell recruitment into the lungs.', ' 5a,b).', 'C3aR antagonist inhibits inflammatory cell infiltration in lung tissue of hACE2-KI mice injected with S1.', 'C3aR blockade limits lung tissue injury and fibrosis induced by SARS-CoV2-derived S1To analyze the full spectrum of the therapeutic potential of complement inhibition, we investigated the effect of C3aRa on lung histologic changes and fibrosis.']","Figure 5 C3aR antagonist inhibits inflammatory cell infiltration in lung tissue of hACE2-KI mice injected with S1. ( ) Representative images and quantification of GR1 neutrophils (red) in CTR (n=4), S1-injected mice at 3d (n=3), and 7d treated (n=6) or not (n=5) with C3aRa. Results are presented as meanSEM. *** <0.001 versus CTR; <0.01 versus S1 3d; <0.001 versus S1 7d. ( ) Representative images and quantification of MAC2 macrophages (red) in CTR (n=4), S1-injected mice at 3d (n=3), and 7d treated or not with C3aRa (n=6 group). Lung structures and nuclei are counterstained with WGA lectin (green) and DAPI (blue), respectively. Scale bars: 20m. Results are presented as meanSEM. *** <0.001 versus CTR; <0.001 versus S1 3d; <0.001 versus S1 7d.",yes
PMC5621804,Figure_1,oa_package/67/29/PMC5621804.tar.gz,"['Tendinosis results in thickening of the tendon, focal or diffuse areas of hypoechogenicity, and preservation of a fibrillar appearance () [10].', 'The CFL originates from the tip of the lateral malleolus; therefore, it is examined with coronal imaging by placing the upper angle of the transducer over the tip of the lateral malleolus (1) [3].', 'The medial deltoid ligament has a triangular shape, originates from the apex of the medial malleolus, and fans over the internal aspect of the talus and calcaneus (2).', 'The AiTFL is the weakest of the syndesmotic ligaments and is the first to yield to forces that create an external rotation of the fibula around its longitudinal axis (3A, C).', 'The AiTFL sometimes appears striated due to fat interposed between the fascicles of the ligament (3B) [8].', 'The ganglion is usually depicted as an anechoic or hypoechoic area with a round or multilobular shape (4).', 'US can also reveal a beak-shaped bony process in short-axis images (5) [39].', 'In this condition, fatty tissue exists between the nerve fascicles, similar to a coaxial cable in the axial plane, and a spaghetti-like appearance can be observed in the coronal plane (6) [42].', ""MR imaging of the ankle and footRadiographics200020 Spec NoS153S179110461699BoonthathipMChenLTrudellDResnickDLateral ankle ligaments: MR arthrography with anatomic correlation in cadaversClin Imaging20113542482123741710JacobsonJAvan HolsbeeckMTMusculoskeletal ultrasonographyOrthop Clin North Am199829135167940578111NazarianLNRawoolNMMartinCESchweitzerMESynovial fluid in the hindfoot and ankle: detection of amount and distribution with USRadiology1995197275278756883712GrantTHKelikianASJerebSEMcCarthyRJUltrasound diagnosis of peroneal tendon tears: a surgical correlationJ Bone Joint Surg Am200587178817941608562013BianchiSDelmiMMoliniLUltrasound of peroneal tendonsSemin Musculoskelet Radiol2010142923062053995514BencardinoJTRosenbergZSSerranoLFMR imaging features of diseases of the peroneal tendonsMagn Reson Imaging Clin N Am200194935051169442315PhilbinTMLandisGSSmithBPeroneal tendon injuriesJ Am Acad Orthop Surg2009173063171941164216RaikinSMEliasINazarianLNIntrasheath subluxation of the peroneal tendonsJ Bone Joint Surg Am2008909929991845139017ButlerBWLanthierJWertheimerSJSubluxing peroneals: a review of the literature and case reportJ Foot Ankle Surg199332134139831897018DavisWHSobelMDelandJBohneWHPatelMBThe superior peroneal retinaculum: an anatomic studyFoot Ankle Int199415271275795196619OdenRRTendon injuries about the ankle resulting from skiingClin Orthop Relat Res1987(216)636920JarvinenTAKannusPMaffulliNKhanKMAchilles tendon disorders: etiology and epidemiologyFoot Ankle Clin2005102552661592291721MaffulliNSharmaPLuscombeKLAchilles tendinopathy: aetiology and managementJ R Soc Med2004974724761545925722AlmekindersLCTempleJDEtiology, diagnosis, and treatment of tendonitis: an analysis of the literatureMed Sci Sports Exerc19983011831190971085523HartgerinkPFessellDPJacobsonJAvan HolsbeeckMTFull- versus partial-thickness Achilles tendon tears: sonographic accuracy and characterization in 26 cases with surgical correlationRadiology20012204064121147724424SchellerADKasserJRQuigleyTBTendon injuries about the ankleClin Sports Med19832631641665270625HaglundPBeitrag zur Klinik der AchillessehneZ Orthop Chir192749495826SofkaCMAdlerRSPositanoRPavlovHLuchsJSHaglund's syndrome: diagnosis and treatment using sonographyHSS J2006227291875184327RodriguezCPGoyalMWasdahlDABest cases from the AFIP: atypical imaging features of bilateral Achilles tendon xanthomatosisRadiographics200828206420681900165928PeetronsPCreteurVBacqCSonography of ankle ligamentsJ Clin Ultrasound2004324914991555862629MorvanGMathieuPBussonJWybierMUltrasonography of tendons and ligaments of foot and ankleJ Radiol2000813613801093088030KumaiTTakakuraYRufaiAMilzSBenjaminMThe functional anatomy of the human anterior talofibular ligament in relation to ankle sprainsJ Anat20022004574651209039231van den BekeromMPOostraRJGolanoPvan DijkCNThe anatomy in relation to injury of the lateral collateral ligaments of the ankle: a current concepts reviewClin Anat2008216196261877347132LagallaRIovaneAMidiriMLo CastoADe MariaMComparison of echography and magnetic resonance in sprains of the external compartment of the ankleRadiol Med199488742748787823033PufferJCThe sprained ankleClin Cornerstone2001338491146473034TakaoMOchiMOaeKNaitoKUchioYDiagnosis of a tear of the tibiofibular syndesmosis: the role of arthroscopy of the ankleJ Bone Joint Surg Br2003853243291272910235HavelPEEbraheimNAClarkSEJacksonWTDiDioLTibial nerve branching in the tarsal tunnelFoot Ankle19889117119285262936AhmadMTsangKMackenneyPJAdedapoAOTarsal tunnel syndrome: a literature reviewFoot Ankle Surg2012181491522285795437NagaokaMSatouKTarsal tunnel syndrome caused by gangliaJ Bone Joint Surg Br1999816076101046373038TakakuraYKitadaCSugimotoKTanakaYTamaiSTarsal tunnel syndrome: causes and results of operative treatmentJ Bone Joint Surg Br199173125128199174539NagaokaMMatsuzakiHUltrasonography in tarsal tunnel syndromeJ Ultrasound Med200524103510401604081640MasonMLPresentation of cases: Proceedings of the American Society for Surgery of the HandJ Bone Joint Surg Am19533527327541JainTPSrivastavaDNMittalRGamanagattiSFibrolipomatous hamartoma of median nerveAustralas Radiol200751 Spec NoB98B1001787517342LeeCHWuJSGoldsmithJDKungJWLipomatosis of the sciatic nerve secondary to compression by a desmoid tumorSkeletal Radiol2013421751175423801100."", '1.', '2.', '3.', '4.', '5.', '6.']","Fig. 1. Tendinosis of the peroneus longus tendon. Long-axis (A) and short-axis (B) ultrasonography of the peroneus longus tendon (arrows) shows diffusely enlarged and hypoechoic swelling of the tendon without tendon fiber discontinuity. There is no evidence of synovial thickening or synovial fluid collection. PL, peroneus longus tendon; PB, peroneus brevis tendon; LM, lateral malleolus; OP, os peroneale.",yes
PMC5376045,Figure_1,oa_package/50/38/PMC5376045.tar.gz,"['(a and Supplementary ', '6 fold, a and Supplementary ', '0 fold, a and Supplementary ', 'We observed marked inhibition in CHOP, BiP and phospho-eIF2 expression in infected HKM (b and Supplementary ', 'We observed that Xes and Dant, inhibitors to IP3R and RyR respectively inhibited the expression of CHOP, BiP and phospho-eIF2 (b and Supplementary ', 'It is evident from c, that alleviation of ER-stress down-regulated calpain-2 expression in A.', 'hydrophila-infected HKM (d).', 'Pre-treatment with the caspase-12 inhibitor, Z-ATAD-FMK inhibited caspase-12 activation (d) and attenuated A.', 'HKM were pre-treated with calpain-2i and we noted that caspase-12 levels were significantly reduced in the infected HKM (d and Supplementary ', 'On the contrary, pre-treatment with Apo and DPI failed to impact calpain-2 activation (c), suggesting superoxide ion generation is downstream to calpain-2 activation.', 'A.']","Figure 1 -induced ER-stress triggers calpain-2 mediated caspase-12 activation in HKM. (a) HKM were infected with and at indicated time p.i. BiP (left panel), CHOP (middle panel) and p-eIF2 (right panel) expression studied by immunofluorescence. TRITC-conjugated secondary antibody used for red fluorescence. (b) HKM were pre-treated separately with Xes, Dant and 4-PBA and expression of BiP (left panel), CHOP (middle panel) and p-eIF2 (right panel) studied by immunofluorescence at 1h, 4h and 24h p.i. FITC-conjugated secondary antibody used for green fluorescence. (c) Representative western blot for calpain-2 expression in lysates of HKM pre-treated separately with 4-PBA, DPI and Apo at 24h p.i. -actin served as the loading control. (d) HKM were pre-treated separately with calpain-2 and Z-ATAD-FMK and at 24h p.i. caspase-12 activity checked. The images are representative of three independent experiments and observed under confocal microscope (40). HKM, uninfected control; HKM + B, HKM infected with ; HKM + 4-PBA + B, HKM pre-treated with 4-PBA for 1h before -infection; HKM + Xes + B, HKM pre-treated with xestospongin C for 1h before -infection; HKM + Dant + B, HKM pre-treated with dantrolene for 1h before -infection; HKM + Z-ATAD-FMK + B, HKM pre-treated with Z-ATAD-FMK for 1h before -infection; HKM + calpain-2 + B, HKM pre-treated with calpain-2 for 1h before -infection; HKM + Apo + B, HKM pre-treated with Apo for 1h before -infection; HKM + DPI + B, HKM pre-treated with DPI for 2h before -infection. 4-PBA, ER-stress protein inhibitor; Xes, IP3R inhibitor; Dant, RyR inhibitor; Z-ATAD-FMK, caspase-12 inhibitor; calpain-2 , calpain-2 inhibitor; Apo and DPI, NADPH Oxidase inhibitor.",yes
PMC11249590,Figure_7,oa_package/76/93/PMC11249590.tar.gz,[],"FIGURE 7 Progress of subdural hematomas by CT surveillance. Initial CT of the head in axial view notes mixed density subdural hematoma with acute and subacute components at the left frontal (A) and right frontal (B) areas. After presentation, the patient underwent left-side craniotomy and evacuation and right-side middle meningeal artery embolization. CT two weeks after embolization noted chronic blood with some decrease of the left-side subdural hematoma (C) and stable size of the right-side subdural hematoma (D). Ten weeks after embolization, acute expansion of the left-side subdural hematomas was noted with a mix of acute and chronic blood (E), however the size of the right-side subdural hematoma continues to decrease and only chronic blood is noted (F). Twelve weeks after embolization, CT of the head noted stable chronic left-sided SDH (G & H), with near resolution of the chronic right-sided SDH (H). All figures show the left side in red and the right side in yellow. CT: computed tomography.",yes
PMC11618033,Figure_6,oa_package/75/b0/PMC11618033.tar.gz,[],"Figure5 Berb promotes host mediated protective response RNA was isolated from infected (SARS-CoV-2 and B.1.617.2) and Berb-treated mice. cDNA was synthesized using applied biosystem c-DNA kit. (A and B) Relative mRNA expression of ISGs Irf3, Tbk1, IFN-, and IFITM (one-way ANOVA; Tukeys multiple comparison test). Bar graph representsSEM (ns=non-significant). (C) relative mRNA expression of chemokine CXCL10, measured by qPCR from lung samples ( = 4) (one-way ANOVA followed by Tukeys, multiple comparison test); bar graph represents as a mean SEM. (D and E) antiviral genes (Oas1g and MX2) profile by RT-PCR from lung samples ( = 4) (one-way ANOVA followed by Tukeys, multiple comparison test). (F and G) The effect of Berb on SARS-CoV and MESR-CoV virus inhibition was determined by neutralization assay. Tablerepresents IC values. See also .",yes
PMC9315689,Figure_2,oa_package/f8/a5/PMC9315689.tar.gz,['Erythematous papules and plaques on the trunk and inferior limbs.'],Figure 2 Erythematous papules and plaques on the trunk and inferior limbs. Lesions vary in size from 0.5 cm to large confluent areas.,yes
PMC6963574,Figure_10,oa_package/26/9f/PMC6963574.tar.gz,[],"Figure 10 Lung macrophage depletion. WT, STAT1 , and STAT6 mice were treated either with PBS-liposomes or clodronate-liposomes intratracheally to deplete macrophages, before and after being infected with 500 L2 larvae. The animals were euthanized at 4 dpi, and the lungs were taken and photographed, ( ) H&E staining was carried out to obtain images at 40 or 100 ( ), and the inflammatory infiltrate areas were measured ( ). Lungs cells were collected, and flow cytometry was used to determine the efficacy of the treatment. Representative dot plots and their respective percentage of F4/80-positive cells of the three strains of mice ( , ) * < 0.05 comparing WT versus STAT1 and STAT6 mice.",yes
PMC9590410,Figure_1,oa_package/9f/13/PMC9590410.tar.gz,"['VRS are typically microscopic pial-lined extensions of the subarachnoid space surrounding vessels entering the brain parenchyma (7) see .', 'Schematic representation of the neurovascular unit: Virchow-Robin spaces encompassing perforating arteries are filled with CSF as they communicate directly with the subarachnoid space (light blue).', 'Representative dorsal plane T1-W slices showing the continuous linear CSF-isointense coursing of the dilated VRS, as well as an exemplary diffusion-weighted apparent diffusion coefficient (ADC) map (Supplementary ), can be found online in the Supplementary material of this article.', '1002836/full#supplementary-materialSupplementary (A,C) Transverse plane T2-W image of a canine brain with bilateral CSF-isointense foci in the ventral forebrain at the level of the rostral commissure.']","Figure 1 Schematic representation of the neurovascular unit: Virchow-Robin spaces encompassing perforating arteries are filled with CSF as they communicate directly with the subarachnoid space (light blue). VRS (red arrow) are enveloped in two leptomeningeal membranes (yellow), the inner leptomeningeal cell layer facing the arterial wall and the outer membrane, contiguous with the pia mater, facing perivascular astrocytic endfeet (green).",yes
PMC8382859,Figure_7,oa_package/bc/6e/PMC8382859.tar.gz,"['7 cm in size in both lobes ().', 'Gross photographs of the explant liver.']",Fig. 7 Gross photographs of the explant liver. There are three types of liver nodules. A 2 cm-sized hepatocellular adenoma of beta-catenin mutated subtype is located at segment IV. Multiple focal nodular hyperplasia nodules measuring up to 7.1 cm in size are scattered over both lobes. Multiple regenerative nodules measuring up to 2.7 cm in size are also present in both lobes.,yes
PMC4990989,Figure_2,oa_package/1c/12/PMC4990989.tar.gz,['.'],"Figure 2. Distribution of LC3 scoring in normal, benign and malignant tissue groups and its correlation with SQSTM1 expression. (A) Cross-tabulations of diffuse LC3-I intensity and LC3-II-positive puncta scores within the normal, benign and malignant tissue groups. Intermed., Intermediate. (B) Examples of LC3 staining patterns and autophagy scoring in tissue samples of 2 non-medullary thyroid cancer patients (1000x magnification). (C) Correlation between number of LC3-II-positive puncta with SQSTM1 expression in non-medullary thyroid cancer tissue specimens (N = 10).",yes
PMC6906848,Figure_2,oa_package/f7/14/PMC6906848.tar.gz,"['As shown in , the expression of PK2 and PKR2 was downregulated in diabetic mice compared with control mice.', 'PKR1 levels were downregulated at 2 and 3 months and upregulated at 4 and 5 months in diabetic mice ().', '001""/>Testicular expression of the PK2/PKR proteins in the diabetic mouse growth process.']","Figure 2 Testicular expression of the PK2/PKR proteins in the diabetic mouse growth process. (a) Typical protein expression of PK2, PKR1, and PKR2 at 2 months. (b) Typical protein expression of PK2, PKR1, and PKR2 at 3 months. (c) Typical protein expression of PK2, PKR1, and PKR2 at 4 months. (d) Typical protein expression of PK2, PKR1, and PKR2 at 5 months. (e) Quantification of PK2 protein expression at different time points. (f) Quantification of PKR1 protein expression at different time points. (g) Quantification of PKR2 protein expression at different time points. = 46 per group. The values are expressed as the mean SEM. < 0.05 vs. the control group.",yes
PMC9187187,Figure_2,oa_package/73/5d/PMC9187187.tar.gz,[],Figure 2 DTI Image showing spinal cord fiber DTI - diffusion tensor imaging,yes
PMC6824304,Figure_6,oa_package/82/76/PMC6824304.tar.gz,"['EAT of both cases and controls had a higher expression of the visceral fat marker omentin-1 (INTLN1) (A).', 'In contrast, the SAT of cases and controls expressed higher levels of the subcutaneous fat marker neuronatin (NNAT) (B).', 'Expression of common inflammatory genes (CCR2, CCL2, IL8, IL6, PAI1, and TNFA) was not significantly different between groups ().', 'Interestingly, expression of 3 members of the orphan nuclear hormone receptor family was increased in the EAT of control patients in comparison with cases (, C E).', 'qRT-PCR verifies downregulation of NR4A1, NR4A2, and NR4A3 in EAT of cases.']","Figure 6 qRT-PCR verifies downregulation of , , and in EAT of cases. ( ) expression was greater in EAT than SAT in both cases and controls. ( ) expression was greater in SAT than EAT in both cases and controls. ( ) expression was significantly decreased in EAT of cases compared with controls. ( ) expression was reduced in EAT of cases in comparison with all other depots. ( ) expression was reduced in EAT of cases in comparison with SAT of cases and EAT of controls. ( ) expression was increased in the SAT of cases compared with other groups. ( ) expression was increased in the SAT of cases compared with EAT. ( ) expression was not different between groups. ( ) expression was not different between groups. ( ) expression was not different between groups. ( ) expression was not different between groups. ( ) expression was not different between groups. ( ) expression was not different between groups ( = 1112 per group; ** < 0.01, and * < 0.05 for indicated comparisons using 2-way ANOVA and Tukeys multiple-comparisons test). In each column, individual subjects are plotted, and error bars show the mean and standard deviation per group. White circles represent controls; black squares represent cases.",yes
PMC8846180,Figure_2,oa_package/a0/36/PMC8846180.tar.gz,"['Subsequently, a transthoracic echocardiogram revealed akinesis of all left ventricular apical segments and apical ballooning with hyperdynamic basal segments ().', 'Echocardiogram in systole (A) and diastole (B), four-chamber views demonstrating movement of the basal segments but akinesis within the apex.']","Figure 2 Echocardiogram in systole ( ) and diastole ( ), four-chamber views demonstrating movement of the basal segments but akinesis within the apex.",yes
PMC7771898,Figure_1,oa_package/14/20/PMC7771898.tar.gz,[],"FIGURE 1 Schematic diagram showing the role of mucins in susceptibility toward COVID19. Different glycosylation patterns on mucins may aid or prevent the entry of SARSCoV2 into the target cells. Different glycosylation patterns on mucins may (1) prevent the binding of the S protein to its receptors (ACE2 and NRP1) on the target cell by steric hindrance and (2) modulate entry of the SARSCoV2 into the target cells by regulating the expression of coreceptors (for example, MUC1 regulates expression of NRP1). The diagram was created with Biorender and Autocad",yes
PMC4324869,Figure_1,oa_package/d0/cd/PMC4324869.tar.gz,"['Patients with suspected hilar obstruction (dilated intrahepatic biliary tree without dilation of extrahepatic bile duct) benefit more from MRI/MRCP which, besides locating the stricture and sometimes identifying the mass, can also map the biliary tree ().', '.']",Figure 1. Patient with obstructive jaundice. (a) MRCP showing a hilar stricture and proximal biliary dilatation. (b) ERCP in the same patient before and (c) after placement of two plastic biliary stents.,yes
PMC10417453,Figure_5,oa_package/4f/36/PMC10417453.tar.gz,"['Among observed cases, only one clear instance of stage 4 periodontitis was detected (ID M 0948; a,b).', 'ID M 0948, male.']","Figure 5 ID M0948, male. ( ) Approx. length120 mm. Fourth-stage periodontitis with local dehiscence (marked by a blue square) of the left mandible and maxilla in the skull of the raccoon dog. ( ) X-ray of fourth-stage periodontitis with local dehiscence (marked by a blue square) of the left mandible and maxilla in the skull of a raccoon dog.",yes
PMC4504606,Figure_1,oa_package/50/db/PMC4504606.tar.gz,[],FIGURE 1 Pathology findings resulted in a diagnosis of the tumor as tongue adenoid cystic carcinoma.,yes
PMC9727799,Figure_2,oa_package/b4/59/PMC9727799.tar.gz,"['Compared to CoV-2 S, a lower concentration of CoV S and a shorter time of treatment were sufficient to trigger ACE2 down-regulation (, A and B, and Supplemental S1E), suggesting that CoV S induced ACE2 down-regulation more efficiently than CoV-2 S.', 'As shown in C, CoV S displayed a stronger binding for ACE2 than CoV-2 S.', 'At day 2 postinfection, hamsters infected with different variants exhibited similar viral titers in the lung, and by day 5, while the viral titers started to decrease in the lungs of hamsters infected with the WA1 and D614G strains, they maintained the same level in those infected with the Beta variant (D).', 'As shown in E, all variants down-regulated ACE2 in lung tissues.', 'Consistent with the in vivo results, purified S proteins containing the D614G, N501Y/D614G, or N501Y/D614G/ 69-70/P681H (Alpha) mutations also down-regulated ACE2 in HEK-293AACE2 cells as efficiently as the S protein from the original WA1 strain (, F and G, and Supplemental S1E).']","FIGURE 2: Down-regulation of ACE2 by the S protein of different SARS-CoV-2 strains. (A, B) CoV S down-regulated ACE2 more efficiently than CoV-2 S. HEK-293A cells were treated with different amounts of VSV-G, CoV S, or CoV-2 S protein for 6 h (A) or with 17.5 g of VSV-G, CoV S, or CoV-2 S protein for the indicated time (B) and then subjected to Western blotting analysis. ACE2 levels were quantified, normalized to that of -tubulin, and are shown in the graphs. Data are mean SEM. = 3 independent experiments. (C) Strep pull-down assay was performed by incubating purified Strep-tagged CoV S or CoV-2 S with lysates of HEK-293A cells, and the associated ACE2 proteins were detected by Western blotting with anti-Flag. Strep-tagged S proteins were assessed by Western blotting with anti-Strep. ACE2 proteins in the cell lysates were examined by Western blotting with anti-Flag. (D) Titers of SARS-CoV-2 variants in the lungs of infected hamsters were determined on days 2 and 5 postinfection. Each hamster was infected with 4 10 TCID of virus. Each dot, square, or triangle represents the viral titer in each lung tissue of infected hamsters. Data are mean SD. = 3, 4, or 5 hamsters per group. (E) SARS-CoV-2 variants down-regulated ACE2 in vivo in lung tissues. Lung tissues were harvested from SARS-CoV-2, SARS-CoV-2 variants, or mock virusinfected hamsters and subjected to Western blotting with anti-ACE2. = 3 individual samples. (F, G) The S protein of CoV-2 variants down-regulated ACE2. HEK-293A cells were treated with various amounts of S proteins from different variants for 6 h and then subjected to Western blotting with anti-Flag. ACE2 levels were quantified and normalized to that of -tubulin and are shown in the graph. Data are mean SEM. = 3 independent experiments.",yes
PMC7484530,Figure_2,oa_package/62/24/PMC7484530.tar.gz,"['The procedure was successful and no additional sessions were needed ().', 'A) Visualisation of the right hepatic artery aneurysm.', 'The patient was discharged fifteen days after admission, maintaining an outpatient follow-up.']",Fig. 2 A) Visualisation of the right hepatic artery aneurysm. B) Control X ray during embolization procedure.,yes
PMC7431516,Figure_7,oa_package/f4/ae/PMC7431516.tar.gz,['MRI showed (1) partial injury of the flexor retinaculum with (2) BME in the medial malleolusTendinopathy and enthesopathyA BME near the tendon may reveal tendinopathy (Figs.'],Fig. 7 A 39-year-old male imaged 10days after an ankle injury on suspicion of deltoid ligament rupture. MRI showed (1) partial injury of the flexor retinaculum with (2) BME in the medial malleolus,yes
PMC9776820,Figure_3,oa_package/f9/be/PMC9776820.tar.gz,"[' presents a comparison of the images obtained using FS, OCMIS, and FFPE for an ILC case with SLN metastasis.', 'Three pathways frozen section (FS), optical section, and formalin-fixed paraffin-embedded (FFPE) section were used for the same raw specimen in a tissue processing series.']","Figure 3 Three pathwaysfrozen section (FS), optical section, and formalin-fixed paraffin-embedded (FFPE) sectionwere used for the same raw specimen in a tissue processing series. With FS-H&E, p-H&E, and FFPE-H&E stains, three individual images were generated.",yes
PMC9547861,Figure_2,oa_package/cd/de/PMC9547861.tar.gz,"[' 2a).', ' 2b).', ' 2c) and VD superior (', ' 2d) quadrant measurements were detected among diagnostic groups.', 'Adjusted macular VD measurements by diagnostic group.', 'Representative VD images from the superficial vascular plexus at the macular region for each diagnostic group are depicted in ']","Figure 2 Adjusted macular VD measurements by diagnostic group. Macular VD differences among diagnostic groups in ( ) temporal, ( ) inferior, ( ) nasal and ( ) superior quadrants. Macular VD measurements are adjusted by age, sex, education, hypertension, diabetes mellitus, heart disease and stroke. CU=cognitively unimpaired; ADD=probable Alzheimers disease dementia; MCI-AD=mild cognitive impairment due to Alzheimers disease; MCI-Va=mild cognitive impairment due to cerebrovascular pathology; n.s.=non-significant.; VaD=vascular dementia; VD=vessel density. Statistical significance was set-up at <0.05.",yes
PMC8665564,Figure_2,oa_package/c8/a5/PMC8665564.tar.gz,"[' 2A, B).', ' 2C, D).', ' 2E).', 'The effects of the N-terminal truncation mutants of sTREM2 on microglial responses.', 'Bound A was detected by MOAB-2 antibodyThe extracellular domain of TREM2 has been reported to bind to the major pathological component of AD, oligomeric amyloid- 1 42 (oA 1 42) with high affinity [17, 18].', ' 2F).', ' 2G), suggesting an impaired binding of the sTREM2 fragment 51 81 to oA 1 42.']","Fig. 2 The effects of the N-terminal truncation mutants of sTREM2 on microglial responses. Schematic diagram of a series of N-terminal truncation mutants of sTREM2 fused with human IgG-Fc. Silver staining analysis of the sTREM2 fragments purified from the conditioned media of transfected HEK293T cells. , Primary microglia were treated with 20nM of Fc alone or the Fc-tagged sTREM2 fragments for 4h. RNA was extracted, and the relative mRNA levels of IL-1 ( ) and TNF ( ) were determined by quantitative real-time PCR. -Actin was used as an internal control ( =35 per group, one-way ANOVA). Microglia were treated with 20nM Fc alone or the Fc-tagged sTREM2 fragments for 24h after GM-CSF withdrawal. TUNEL was performed to analyze cellular apoptosis ( =3, one-way ANOVA). The binding affinity between oA and either of Fc, the Fc-tagged sTREM2 fragment 4181, 5181, 1157 at indicated concentrations were analyzed by solid-phase binding assay ( =4, one-way ANOVA). Dot blot of oA binding to either of Fc, sTREM2 fragment 4181, 5181, 1157. A PVDF membrane was spotted with each of the sTREM2 fragment and then incubated with 100nM oA . Bound A was detected by MOAB-2 antibody",yes
PMC3499034,Figure_5,oa_package/5e/97/PMC3499034.tar.gz,"['We found that DLPFC TMS was associated with a significant elevation of BOLD signal in multiple dorsal cortical areas, whereas mPFC TMS was associated with a significant elevation of BOLD signal in multiple medial and limbic subcortical areas ().', 'Integrating impaired structural integrity in cocaine users with functional activity.']","Figure 5 Measuring functional connectivity via simultaneous brain imaging and brain stimulation. Through the use of transcranial magnetic stimulation in the magnetic resonance environment, we are able to selectively stimulate cortical brain regions and measure the BOLD response or changes in blood flow secondary to the stimulation. BOLD, blood-oxygen level dependent.",yes
PMC7526387,Figure_3,oa_package/65/58/PMC7526387.tar.gz,"['3a h).', '3b) and ghrelin (', '3c) are hormones that have opposing actions on energy balance via signaling in the hypothalamus.', 'Sex-specific effects of HF diet and AD on plasma levels of diabetes-associated markers and expression of hypothalamic peptides that regulate feeding.', '01Insulin (', '3a) and glucagon (', '3d) are hormones involved in maintaining homeostatic blood glucose levels.', '3e) and glucagon-like-peptide 1 (GLP-1; ', '3f) are two hormones with opposing actions on postprandial glucagon secretion, such that GIP enhances it and GLP-1 suppresses it.', '3g) is synthesized by adipose tissue, and increases in PAI-1 can result from inflammatory signals and contribute to a pro-thrombotic state and the development of metabolic disease [71].', '3h) is a hormone secreted by adipose tissue, as well as immune and epithelial cells, which suppresses insulin s ability to promote cellular glucose uptake.', '3i n).', '3i) and agouti-related peptide (AgRP; delayed, longer-acting orexigenic peptide; ', '3j) [72], pro-opiomelanocortin [POMC; an anorexigenic precursor protein and member of the central melanocortin system; cleaved to -melanocyte stimulating hormone ( -MSH)] [73] (', '3k), leptin receptor (LepR; binds the anorexigenic hormone, leptin; ', '3l), melanocortin receptor 4 (MCR4; a G protein-coupled receptor that binds -MSH; ', '3m), and fibronectin type III domain-containing protein 5 (FNDC5; the precursor of irisin, a thermogenic adipomyokine; ', '3n) [74].']","Fig. 3 Sex-specific effects of HF diet and AD on plasma levels of diabetes-associated markers and expression of hypothalamic peptides that regulate feeding. Plasma concentrations of diabetes-related markers. Blood was collected following a 5h fasting period just prior to euthanasia, and assayed for insulin, leptin, ghrelin, glucagon, gastric inhibitory polypeptide (GIP), glucagon-like-peptide 1 (GLP-1), plasma plasminogen activator inhibitor-1 (PAI-1), and resistin. = 8/group for all plasma markers. Gene expression levels in homogenate of the whole hypothalamus related to energy balance. Hypothalamus was collected following a 5h fasting period just prior to euthanasia, and assayed for neuropeptide Y (NPY), agouti-related peptide (AgRP), pro-opiomelanocortin (POMC), leptin receptor (LepR), melanocortin receptor 4 (MCR4), fibronectin type III domain-containing protein 5 (FNDC5). = 58/group for all gene expression analyses in the hypothalamus. * = diet effect < 0.05; ** = diet effect < 0.01; ^ = AD effect < 0.05; ^^ = AD effect < 0.01",yes
PMC5741276,Figure_2,oa_package/e8/e9/PMC5741276.tar.gz,"['Proton Stereotactic Body Radiation Therapy PlansThe proton treatment plan is displayed for the A) right renal target volume and B) left renal target volume, with lesions treated with two separate isocenters.']","Figure 2 Proton Stereotactic Body Radiation Therapy Plans The proton treatment plan is displayed for the A) right renal target volume and B) left renal target volume, with lesions treated with two separate isocenters. Treatment fields are displayed, with each volume being treated with posterior and left posterior oblique beams. The right planning target volume is contoured in orange, whereas the left planning target volume is contoured in magenta. The radiation dose is displayed as a color wash. Color coding: red = 100% to blue = 10% of 30 Gy in 6 Gy fractions. C) A plan summary of the right and left treatment courses is depicted.",yes
PMC11224543,Figure_1,oa_package/05/28/PMC11224543.tar.gz,"['Computed Tomography Angiogram of the ChestA transthoracic echocardiogram revealed an ejection fraction of 55%, concentric left ventricular (LV) hypertrophy, severe RV dilation, diastolic septal flattening, and severely elevated RV systolic pressure of 72 mmHg.']",Figure 1 Computed Tomography Angiogram of the Chest,yes
PMC5566374,Figure_9,oa_package/e4/d3/PMC5566374.tar.gz,"[' 9a,b).', 'There was no major change in ganglion cell staining detected by Brn-3a antibody in control (a) and diabetic (b) specimens.', '\nDiscussionAlthough T2D rat models such as ZDF, OLETF, SDT rats etc.']","Figure 9 There was no major change in ganglion cell staining detected by Brn-3a antibody in control ( ) and diabetic ( ) specimens. DAPI is used as a nuclear staining. ONL: outer nuclear layer, INL: inner nuclear layer, GCL: ganglion cell layer. : 20m.",yes
PMC7399179,Figure_2,oa_package/be/bb/PMC7399179.tar.gz,"['FlowJo software (Treestar) was used to quantify cell populations from human SVCs, which were gated according to Supplementary .', 'The gating strategy is depicted in Supplementary .', '04; A).', 'Preadipocyte phenotype.', 'Full gating strategy is shown in Supplementary .', 'We stratified preadipocytes into three subtypes according to CD34 and CD29 expression (B) as previously performed by Raajendiran et al.', 'Shown in C, these three populations are labeled CD34hi (CD29+CD34hi), CD34lo (CD29+CD34lo), and CD34 (CD29+CD34 ).', 'These populations also express variable CD29 expression, with the CD34 population expressing the highest level of CD29 (B).', 'EX (C).', '59; D).']","Figure 2 Preadipocyte phenotype. Effects of exercise on total preadipocytes in the stromovascular fraction. Representative plot showing preadipocyte subtype selection on CD34 and CD29 expression. Full gating strategy is shown in . Effects of exercise on preadipocyte subtypes (CD34 , CD34 , and CD34 ) expressed as %SVC. Effects of exercise on preadipocyte subtypes expressed as %total preadipocytes. = 10. Data are mean SD. < 0.05 by Sidaks multiple comparison. SVC, stromal vascular cells.",yes
PMC9464768,Figure_4,oa_package/83/9d/PMC9464768.tar.gz,['Asymmetric ovarian enlargement is the most commonly described sonographic finding suggestive of ovarian torsion.'],"Fig. 4 Transvaginal pelvic ultrasound showing large left ovarian cyst, and adjacent inferior small bladder.",yes
PMC4553915,Figure_3,oa_package/aa/18/PMC4553915.tar.gz,['Neovessel formation in hemi-RVO tissue involves vascular EC precursors.'],"Fig. 3 Neovessel formation in hemi-RVO tissue involves vascular EC precursors. CD117 (c-kit) was used as a marker for VESCs (left panel, red). Larger magnification shows cytoplasmic staining (arrow heads) in lumen-lining vessel structures (middle panel). Immunoreactivity for the active proliferation marker Ki67 is also shown (right panel, red).",yes
PMC4965011,Figure_3,oa_package/3e/c1/PMC4965011.tar.gz,"['g002""/> displays an example of one EB immunized-challenged mouse during the course of the study; slight pathology was seen throughout the study although overall scores were lower than the na ve-challenged group.', 'g003MRI images of an example EB immunized-challenged mouse at all time points during the study (n = 10 mice in EB immunized-challenged group).']",10.1371/journal.pone.0160055.g003,yes
PMC3535371,Figure_6,oa_package/a3/7b/PMC3535371.tar.gz,"[' 6) and connects the myofibrillar apparatus with myonuclei, other cell organelles, and the extracellular matrix via the subsarcolemmal cytoskeleton [27, 160].', '', 'In desminopathies, mutant desmin leads to structural and functional changes of the extrasarcomeric cytoskeleton, pathological protein aggregation, mitochondrial abnormalities, and signs of myofibrillar degeneration\nMolecular interaction partnersDesmin exerts its multiple functions through direct and indirect binding to various other cellular molecules.']","Fig.6 Schematic of the desmin IF network in relation to the myofibrillar apparatus. In desminopathies, mutant desmin leads to structural and functional changes of the extrasarcomeric cytoskeleton, pathological protein aggregation, mitochondrial abnormalities, and signs of myofibrillar degeneration",yes
PMC3205758,Figure_11,oa_package/a4/f8/PMC3205758.tar.gz,[],Figure 11 Carcinoid tumour. Contrast-enhanced MDCT shows a spiculated soft tissue mass within the small bowel mesentery (arrow). The central calcification and soft tissue projections extending from the mass are typical of the associated desmoplastic reaction.,yes
PMC8293917,Figure_3,oa_package/6d/38/PMC8293917.tar.gz,"['No significant changes were observed in the number of late stage NPCs, which characteristically express Tbr2, between WT and Thy1-aSyn mice ().', 'Moreover, the number of neurons as identified by Tbr1 or NeuN immunofluorescence did not differ significantly in the Thy1-aSyn mice when compared to their WT littermates at both stages analyzed ().', 'This is demonstrated as percentage increase in numbers of NPCs and neurons between genotypes across ages ().']","FIGURE 3 Quantification of Tbr1 and NeuN positive cells in the hippocampal dentate gyrus of wildtype and Thy1-aSyn mice. Immunofluorescence for Tbr1 (red) and DAPI staining (blue) and merged images of 6-month-old wildtype [WT, ] and Thy1-aSyn [asyn, ] mice. Number of Tbr1 + cells in the granular cell layer (GCL) and subgranular zone (SGZ) of 6-month (mo)-old wildtype (WT) and Thy1-aSyn (asyn) mice. Immunofluorescence for NeuN (red) and DAPI staining (blue) and merged images of 16-month-old wildtype [WT, ] and Thy1-aSyn [asyn, ] mice. Number of NeuN + cells in GCL and SGZ of 6 month-old wildtype (WT) and Thy1-aSyn (asyn) mice. Change (percent) in number of PCNA+, Pax6+/PCNA+, Tbr2+/PCNA+ cells and neurons between 6 and 16 month-old Thy1-aSyn (asyn) and wildtype (WT) mice. Data represent mean SEM. (WT; 6 month-old, = 6, 16 month-old = 6; asyn, 6 month-old, = 6, 16 month-old = 5; Students -test). Scale bars, 50 m.",yes
PMC5999660,Figure_2,oa_package/56/23/PMC5999660.tar.gz,"['037, 1 2 2 RKIP A Western blot B RKIP * P 0.']","2 RKIPAWestern blotBRKIP < 0.05 The expression of RKIP in lung squamous cell carcinoma tissues and adjacent cancer tissues. A: Results of Western blot; B: The relative expression changes of RKIP in squamous cell carcinoma tissues and adjacent cancer tissues. : adjacent cancer tissues vs squamous cell carcinoma tissues, < 0.05",yes
PMC5424110,Figure_5,oa_package/09/b0/PMC5424110.tar.gz,"['As shown in , compared with the vehicle-treated group, the group treated with 25 mg/kg of MPT0G009 daily from days 2 to 21 had significant reductions in paw swelling (a), paw volume (b) and arthritis scores (c).', 'MPT0G009 treatment resulted in significant decreases in the serum levels of IL-1 and IL-6, as did SAHA and indomethacin treatments (d).', 'Furthermore, as shown in e, safranin O staining of rat ankle joints showed that MPT0G009 treatment markedly reduced cartilage degradation, and hematoxylin and eosin staining showed that MPT0G009 treatment significantly reduced leukocyte infiltration, synovitis and apparently ameliorated the decrease of osteoblasts.', 'In addition, micro-computed tomography (micro-CT) scans showed that MPT0G009 treatment ameliorated bone destruction (e) and prevented the decrease of bone mineral density (BMD) and bone mineral content (BMC) (f).', 'org/1999/xlink"" xlink:href=""cddis2014133f4""/>MPT0G009 inhibits the development of arthritis in an AIA model.']","Figure 5 MPT0G009 inhibits the development of arthritis in an AIA model. ( ) After the onset of arthritis (as described in the Materials and methods section), rats were orally treated with either the vehicle (MPT0G009; 25mg/kg), SAHA (200mg/kg) or the positive control indomethacin (1mg/kg) from days 2 to 21 (19 days). Subsequently, swelling of both hind paws was photographed. ( ) Hind paw volumes in the indicated group of rats were measured using a digital plethysmometer on the indicated day after AIA induction. ( ) Arthritis scores on day 21. ( ) Serum levels of interleukin (IL)-1 (left panel) and IL-6 (right panel) on day 21 as measured by enzyme-linked immunosorbent assay (ELISA). ( ) Top five rows: photomicrographs of ankle joint sections from the different groups stained with hematoxylin and eosin, safranin O, immunohistochemically stained with an anti-acetyl H3 antibody or TRAP stain. Arrows indicate osteoblasts (OB); arrowheads indicate osteoclasts (OC). Bottom panels: micro-CT results (arrows indicate bone erosion). ( ) The BMD (in mg/mm ) and the BMC (in mg) values for the bone tissue of the tarsus were analyzed using CT Analysis Software. Results in ( and ) are the meansS.E.M.'s for five independent experiments. * <0.05 compared with the corresponding day 0 value ( ); <0.05 compared with the vehicle-treated control ( ); ** <0.01 and *** <0.001 compared with basal groups ( )",yes
PMC4657381,Figure_3,oa_package/d2/91/PMC4657381.tar.gz,"[' 3).', 'In the mice, we observed fivefold higher enhancement of inflammatory response in brain stem and cervical canal in progression of the disease than in modulation of mGluR5 expression in the same areasQuantitative analysis of modulation of mGluR5 expression and inflammatory response in the spinal cord and lungs during progression of the disease.', ' 3 and 4).']","Fig. 3 PET and fused PET/CT images of the distribution of [ C]PBR28 accumulation in ALS mouse at the stage 3. Studies of inflammatory response using [ C]PBR28 (240Ci i.v.) were conducted in the same mouse as above (Fig. ) 2days after the study of mGluR5 expression. High accumulation of [ C]PBR28 was seen in lungs, hindbrain, brain stem, and in different parts of spinal cord, indicating activated microglia and expression of translocator protein (TSPO). In the mice, we observed fivefold higher enhancement of inflammatory response in brain stem and cervical canal in progression of the disease than in modulation of mGluR5 expression in the same areas",yes
PMC3155083,Figure_1,oa_package/ab/34/PMC3155083.tar.gz,"['Test article-related increases in ALP level were observed in all DAS181-treated groups of male rats and in females in group 5 on day 14 as illustrated in .', 'FIG.']","FIG. 1. Group mean ALP levels. (A) Mean (SD) ALP values in male rats; (B) mean ALP values in female rats. For each sex, 15/group for the control, 1.5 and 3.0 mg/kg dose groups and 10/group for the 0.5 and 1.0 mg/kg dose groups at both time points (except where = 14 control females on day 29). Asterisks indicate significant difference between the control and treated groups, * < 0.05, ** < 0.01, *** < 0.001.",yes
PMC11603663,Figure_1,oa_package/5c/ad/PMC11603663.tar.gz,"[' 1A).', '', 'P-values were determined using one-way ANOVA followed by Tukey Kramer multiple comparison tests (n = 3)To assess the neurotoxic effects resulting from ubiquitous overexpression of hTDP-43M337V, we genetically ablated the floxed stop sequences from both alleles (CAG-hTDP-43M337V-+/+).', ' 1A, Additional file 4: ', ' 1A, black arrows) were used to detect Cre-dependent recombination.', ' 1B, bottom).', ' 1C).']","Fig.1 Generation and characterization of oligodendrocyte-specific human TDP-43 transgenic mice. Left: Schematic of the murine locus, the gene-targeting vector for inserting the floxed human TDP-43 expression cassette, and the resultant mutant locus after homologous recombination. Human TDP-43 cDNA under the control of the CAG promoter, interrupted by a neomycin resistance gene (Neo ) and polyA sequences flanked by loxP sequences, was integrated into the locus. A Myc-Tag was inserted at the N-terminal end of hTDP-43 cDNA. DTA: diphtheria toxin A fragment; WPRE: woodchuck hepatitis virus posttranscriptional regulatory element. Right: Schematic of crossbreeding to generate ; hTDP-43 -fl*/fl mice. Asterisks indicate recombinant alleles. Representative genotyping images of nontransgenic mice (nTg), hTDP-43 -fl/fl mice (lox), ; hTDP-43 -fl*/fl mice [Cre()] and ; hTDP-43 -fl*/fl mice [Cre(+)] from PCR. Genotyping results of Cre recombinase (top), the mutant locus carrying the floxed TDP-43 cassette (middle), and Cre-dependent recombination (bottom). Image showing Cre-dependent recombination also occurring in Cre(). White and black arrows in ( ) indicate specific primers for PCR of the mutant locus and Cre-dependent recombination, respectively. Representative immunoblotting images showing Myc-hTDP-43 expression. Lysates from the corpus callosum and lumbar spinal cord of mice with the specified genotype were subjected to immunoblotting using anti-Myc, anti-TDP-43, or anti--actin antibodies. Asterisks denote nonspecific bands. Relative myc expression levels are presented as meansstandard errors of the mean (SEMs). -values were determined using one-way ANOVA followed by TukeyKramer multiple comparison tests (n=3)",yes
PMC11381454,Figure_3,oa_package/20/3a/PMC11381454.tar.gz,"['', 'The MRI concluded that there was a narrow degenerative lumbar canal aggravated by compression at the L4-L5 level by an extradural cystic formation continuing with the right posterior L4-L5 joint in favor of a synovial facet cyst ().']","Fig. 3 Lumbar spine magnetic resonance imaging. A: T2- sagittal plane showing a right synovial facet cyst at level L4-L5. B: T2- axial plane showing a right synovial facet cyst at level L4-L5, compressing the right L5 root and repressing the dural sheath.",yes
PMC9096465,Figure_4,oa_package/22/93/PMC9096465.tar.gz,"['Axial T1-weighted fat-suppressed MR images following administration of gadolinium at the level of the upper to mid chest (A) and lower chest (B).', 'DiscussionExtramammary findings, including artifacts and anatomic variations, can sometimes be the cause of diagnostic pitfalls when interpreting mammograms.']","Fig. 4 Axial T1-weighted fat-suppressed MR images following administration of gadolinium at the level of the upper to mid chest (A) and lower chest (B). More superiorly, there is a large cleft between the medial and lateral fibers of the left pectoralis muscle (red arrow). The fibers fuse more inferiorly but are diminutive in caliber relative to the right side (yellow arrow).",yes
PMC11307630,Figure_7,oa_package/1d/a4/PMC11307630.tar.gz,[' 7High risk for invasive coronary angiography (ICA) post coronary artery bypass graft (CABG).'],"Figure7 Cardiac computed tomography angiography (CCTA) to evaluate for graft patency in a patient with known (severe) native coronary artery disease and left ventricular thrombus ( arrow). CCTA, rather than ICA, was done preferentially to evaluate the patients grafts without having to instrument the heart or hold oral anticoagulation, reducing the risk for embolic stroke. Patency of all grafts is noted, including a LIMA to the LAD, SVG to the Dx branch, and SVG to the RCA ( ). Dx, diagonal branch (coronary artery); LAD, left anterior descending (coronary artery); LIMA, left internal mammary artery; RCA, right coronary artery; SVG, saphenous vein graft.",yes
PMC10652557,Figure_1,oa_package/00/15/PMC10652557.tar.gz,[],Figure1 The trocar layout and the position of the patients and the operative staff.,yes
PMC2242831,Figure_1,oa_package/fc/b1/PMC2242831.tar.gz,"['We found that Meth concentrations of 50 M or higher rapidly collapsed acidic organelle pH gradients in dendritic cells (A 1B).', '8) (C).', 'As predicted with alkalizing agents, Meth also disrupted MIIC structure [36], producing large organelles ( 1 m diameter) devoid of internal vesicles (right panels) with LAMP-1 and MHC II staining confined to the limiting membrane (D and 1E).', 'Chloroquine (Clq) [10 or 20 M], another weak base was used as a control showed similar effects to Meth as described above (A 1E).', 'Meth Induces Rapid Alkalization of Acidic Organelles, and Changes in Endosomal Structure in Dendritic Cells(A) Morphometric analysis of the decrease in acridine orange staining after 10 min treatment.', 'This is consistent with the effects of low concentrations of Meth tested on lysosomal pH in C, with alkalinization of 1 pH unit, which would effectively inhibit lysosomal protease activity.']","Figure 1 Meth Induces Rapid Alkalization of Acidic Organelles, and Changes in Endosomal Structure in Dendritic Cells (A) Morphometric analysis of the decrease in acridine orange staining after 10 min treatment. Similar results were obtained in three independent experiments. (Statistics = 2535, mean SEM, ** < 0.001, *** < 0.0001, two-tailed ANOVA.) (B) Progressive quenching of cytosolic acridine orange staining over 4 h in the absence () or presence () of 50 M Meth. < 0.001 for all time points versus control ( = 2535, mean SEM, Student's two-tailed t-Test). (C) Acidic organelle pH in cells exposed to Meth for 10 min measured by ratiometric measurement. (Statistics = 3090 cells, mean SEM, ** < 0.001, *** < 0.0001, two-tailed ANOVA.) (D) Representative electron micrographs showing multivesicular endosomal compartments (arrows) in control, Clq- and Meth-treated cells at 4 h. Scale bar, 200 nm. (E) Ultra-thin cryosections of control, Clq- and Meth-treated dendritic cells as in (D), immunolabeled for LAMP-1 (15 nm gold particles) and MHC class II (10 nm gold particles). Scale bar, 200 nm.",yes
PMC9139560,Figure_1,oa_package/38/ba/PMC9139560.tar.gz,"['Application of Mouse Testes depicts the representative in vivo Cr-CEST images of the mouse testes.', '8 ppm, the testes displayed a higher MTR value than other tissues (B,C).', 'S120014Representative in vivo Cr-CEST images of control mouse testes.']","Figure 1 Representative in vivo Cr-CEST images of control mouse testes. ( ) Anatomical T WI image. The white arrow shows the testis. The white dotted arrow shows the testicular epithelium. ( ) MTR asymmetry maps at 1.8 ppm of . ( ) MTR asymmetry maps at 1.8 ppm of . MTR, magnetization transfer ratio; , conventional analysis metric magnetization transfer ratio; , apparent exchange-dependent relaxation; and Cr-CEST, creatine chemical exchange saturation transfer.",yes
PMC7191307,Figure_1,oa_package/00/76/PMC7191307.tar.gz,"['Alveolar sarcoidosis.', '1Adenocarcinoma in situ.', '2Invasive adenocarcinoma.', '3Pulmonary lymphoma.', '4Post-transplant lymphoproliferative disease.', '5Metastatic adenocarcinoma of the pancreas.', '6Lipoid pneumonia.', '7Pulmonary hemorrhage.', '8Pulmonary alveolar proteinosis.']","Figure 1 Alveolar sarcoidosis. A and B: Non-contrast computed tomography of the chest, lung window at baseline (A) and after 5 mo (B) in a 46-year-old man with cough and biopsy-proven sarcoidosis. Pleural-based consolidative opacity remained unchanged (arrows). Typical sarcoidosis findings of peribronchovascular and perilymphatic nodularity can be well-seen in the follow-up image (arrowhead in B); C: The liver was also involved with subtle hypodense lesions in the right and left hepatic lobes (arrows).",yes
PMC3784266,Figure_3,oa_package/96/10/PMC3784266.tar.gz,"['MRI of the pelvis () showed the extension of the lesions with soft tissue involvement of the right thigh and of the left gluteal region.', '002""/>Magnetic resonance imaging: (a) coronal image of the right proximal femur shows the soft tissue involvement (arrow) of the lesser trochanteric region, and (b) axial image of the pelvis demonstrates the soft tissue involvement of the right gluteal region (arrow) nearby the iliac wing and the sacroiliac joint.']","Figure 3 Magnetic resonance imaging: (a) coronal image of the right proximal femur shows the soft tissue involvement (arrow) of the lesser trochanteric region, and (b) axial image of the pelvis demonstrates the soft tissue involvement of the right gluteal region (arrow) nearby the iliac wing and the sacroiliac joint.",yes
PMC7255489,Figure_4,oa_package/69/f0/PMC7255489.tar.gz,['A five-year-old girl with nonisolated congenital vertical talus.'],Fig. 4 A five-year-old girl with nonisolated congenital vertical talus. (a)(c) Radioclinical relapse (incomplete correction) three years after first revision open reduction surgery on right foot.,yes
PMC8091422,Figure_6,oa_package/3c/8e/PMC8091422.tar.gz,"['For example, positive cell counts for all 4 stains were clustered tightly in colon and kidney for all 3 stainers (), while average OD values were closely packed only for kidney ().', '1177_0192623320970534-fig6""/>.', 'These OD differences do not impact analysis of IHC data where the final interpretation is based on labeling of a specific tissue feature (eg, counts of positive cells; see ).']","Figure 6. Precision (repeatability and reproducibility) of IHC staining depends principally on the biomarker and tissue rather than the stainer. Each dot represents the number of positive cells in 1 section. Instrument models: AutostainerPlus Link, Nos. 0010 and 0083; Autostainer Link 48, No. 0151. IHC indicates immunohistochemical.",yes
PMC5508523,Figure_5,oa_package/07/81/PMC5508523.tar.gz,"['After five days of training, mice landed on the platform in less than 30 s except AT-NRF2-KO mice, who took significantly longer (A).', 'On the fifth day, the platform was removed and the mice were also tested for their memory of the platform location (B).', 'NRF2 deficiency impairs long term potentiation, and spatial learning and memory.', 'Further exploration of cognitive function was performed electrophysiologically for hippocampal long term potentiation (LTP) in 6-months-old mice.', 'The synaptic transmission in the neurons of the dentate gyrus was recorded in response to performant path stimulation (C).']","Fig. 4 A, scheme showing APP processing by -, - and -secretase and the location of the epitopes recognized by the antibodies employed in western blot analysis. (SF, soluble fraction and MF, membrane fraction). B, immunohistochemical analysis of APP/A expression in hippocampus of 11-month old AT-NRF2-WT and AT-NRF2-KO mice stained with anti 4G8 antibodies in CA1 and subiculum. C and D, immunoblots with the anti-A antibody as indicated SF and MF fractions from hippocampal homogenates of AT-NRF2-WT and AT-NRF2-KO mice. The right two lanes show samples from APP/PS1 and wild type mice as positive and negative controls, respectively. The asterisk in D indicates a non-specific band present in all samples including the negative control. -actin detection demonstrates similar loading across lanes. E, densitometric quantification of blots depicted in C and D. Data are mean SEM (AT-NRF2-WT = 5 males; AT-NRF2-KO = 4 males and 1 female). Statistical analysis was performed with a Student's -test. *p0.05, comparing AT-NRF2-WT and AT- NRF2-KO groups.",yes
PMC6897346,Figure_4,oa_package/6a/a3/PMC6897346.tar.gz,"['Three-dimensional (3D) reconstruction of the aorta showing branches arising from aorta, as first bicarotid trunk, as second left subclavian artery and as third aberrant right subclavian artery.']","Figure 4 Three-dimensional (3D) reconstruction of the aorta showing branches arising from aorta, as first bicarotid trunk, as second left subclavian artery and as third aberrant right subclavian artery. The celiacomesenteric trunk (arrow) is also present.",yes
PMC8430695,Figure_3,oa_package/9f/67/PMC8430695.tar.gz,"['Effects of Oligomeric and Fibrillary A 42 Peptide on Proliferation of Differentiating hNS1 CellsTo study the effects of proliferation after treatment of oligomeric and fibrillary A 42 peptide, the cultures were analyzed using the cell cycle marker Ki67 and the proliferation marker BrdU (A,F).', '1% in vehicle group (A,C).', '05; n = 3) of cells were Ki67+ (C).', '05; n = 3) of cells, were positive for the cell cycle marker (C).', 'The results were confirmed by qRT-PCR where we observed a decline in MKi67 mRNA levels at all concentrations tested (D).', 'In the case of BrdU (B,E), our results confirmed that 10 1.', '05; n = 3) compared to control groups (E).', '5% in the vehicle group (F,H).', '05; n = 3) (H).', 'The results were confirmed by qRT-PCR (I).', 'Finally, after a 2 h pulse of BrdU (G), our results confirmed that 9 1.', '05; n = 3) (J).', 'Contrary to the effects observed for oligomeric A 42 peptide, all these results suggest that A 42 peptide in its fibrillary form does not appear to have significant effects on the proliferation of hNS1 cells (K,L).', 'Oligomeric and fibrillary A 42 affects cell cycle of differentiating hNS1 cells.']","Figure 3 Oligomeric and fibrillary A42 affects cell cycle of differentiating hNS1 cells. ( ) Representative images showing Ki67 immunoreactivity (green) after oligomeric A42 treatment. ( ) Microphotographs with immunoreactivity for BrdU (red) after oligomeric A42 treatment. ( ) Percentage of Ki67+ cells in the different experimental groups after oligomeric A42 treatment. ( ) Relative expression levels of mRNA determined by qRT-PCR analysis after oligomeric A42 treatment. ( ) Percentage of BrdU+ cells in the different cellular groups after oligomeric A42 treatment. ( ) Representative images showing Ki67 immunoreactivity (green) after fibrillary A42 treatment. ( ) Microphotographs with immunoreactivity for BrdU (red) after fibrillary A42 treatment. ( ) Percentage of Ki67+ cells in the different experimental groups after fibrillary A42 treatment. ( ) Relative expression levels of mRNA determined by qRT-PCR analysis after fibrillary A42 treatment. ( ) Percentage of BrdU+ cells in the different cellular groups after fibrillary A42 treatment. ( ) Percentage of Ki67+ cells/total cells comparing both added forms. ( ) Percentage of BrdU+/total cells comparing both added forms. Nuclei were counterstained in blue with Hechst. Scale bar, 50 M. Data are represented as means SD of at least three different experiments ( = 3). Statistical significance of one-way ANOVA with post hoc Tukey test; * < 0.05; ** < 0.01; *** < 0.001; ns = not significant vs control groups.",yes
PMC3827469,Figure_2,oa_package/05/6a/PMC3827469.tar.gz,"['Images were therefore partitioned into non-overlapping 4096x4096-pixel tiles to accommodate analysis requirements on a single machine and to enable parallel analysis (A).', 'Following the workflow illustrated in B, we segmented approximately 200 million nuclei from 117 GBMs and represented each nucleus with a set of complementary features from four categories: morphometry, intensity, texture, and gradient statistics (A).', 'g002Overall schema of image analysis pipeline for GBM nuclei analysis.', 'This enables the separation of foreground from normalized background with a simple threshold-based mechanism, which is key for efficiently segmenting nuclei with distinct features (C).', 'Using the converged coefficients from this regression model, we calculated NS for each nucleus identified in images (D).']",10.1371/journal.pone.0081049.g002,yes
PMC6815004,Figure_2,oa_package/cf/5e/PMC6815004.tar.gz,"[' 2, ', 'Lower leg muscle and cardiac MRI characteristics in NLSDM patients.', '(f) Cardiac MRI showed dilated cardiomyopathy\nMuscle fatty infiltration score on MRI of upper and lower legs.']","Fig. 2 Lower leg muscle and cardiac MRI characteristics in NLSDM patients. MRI revealed diffuse fatty infiltration, predominantly involving the gluteus maximus at the pelvic level. , The long head of the biceps femoris, semimembranosus and adductor magnus were moderately-to-severely affected. The rectus femoris, gracilis and sartorius were relatively preserved. , The soleus and medial head of the gastrocnemius were severely affected. The anterior and posterior tibialis were relatively preserved. ( ) Cardiac MRI showed dilated cardiomyopathy",yes
PMC5009300,Figure_5,oa_package/4a/8b/PMC5009300.tar.gz,"['In db/db mice, the mesangial matrix fraction increased significantly (a).', 'Fibronectin content in the renal cortex also increased visibly, as indicated by Western blot and immunohistochemistry (b d).', 'AS-IV treatment was found to reduce mesangial matrix fraction and the level of fibronectin but not to a significant degree (a,b).', 'org/1999/xlink"" xlink:href=""srep32545-f4""/>The mesangial matrix fraction (n = 6 per group) was significantly higher in db/db mice (a).', 'Fibronectin content in the renal cortex was also visibly higher, as indicated by Western blot analysis (n = 4 per group) and immunohistochemistry (b d).', 'AS-IV treatment was found to reduce mesangial matrix fraction and fibronectin level but not to a significant degree (a,b).']","Figure 5 The mesangial matrix fraction (n=6 per group) was significantly higher in db/db mice ( ). Fibronectin content in the renal cortex was also visibly higher, as indicated by Western blot analysis (n=4 per group) and immunohistochemistry ( ). AS-IV treatment was found to reduce mesangial matrix fraction and fibronectin level but not to a significant degree ( ). * <0.05 and *** <0.001 relative to the wild type group.",yes
PMC5850605,Figure_3,oa_package/89/16/PMC5850605.tar.gz,"['In addition, for chemokines, there was a positive correlation between CCL2 levels and ACKR2 expression (A) and while no overall correlation was detected with CCL3 (', '3B), patients expressing ACKR2 levels above the median of the whole population display significantly higher CCL3 levels than those below the median (C).']","F Correlation of ACKR2 expression in peripheral blood mononuclear cells with ACKR2-binding chemokines Peripheral blood mononuclear cells (PBMCs) and paired plasma samples were taken from healthy controls, PsA patients, RA patients or early RA patients. Atypical chemokine receptor 2 (ACKR2) expression was measured using qPCR with absolute quantification and normalized to 10 copies of TATA-binding protein (TBP). Plasma was analysed by 30-plex Luminex analysis. , ) ACKR2 expression is shown correlated with CC chemokine ligand (CCL) 2 ( ) and CCL3 ( ). ( ) CCL3 levels between ACKR2-high and ACKR2-low patients. Correlation analysis was performed using Spearmans correlation coefficient.",yes
PMC11554683,Figure_6,oa_package/2a/17/PMC11554683.tar.gz,"['6A) and 293T cells (', 'The interaction between GNG5 and PS1 promoting A 42 production can occur in early endosomes.', 'To investigate this propose, we firstly confirmed total Rab5 levels and western blot revealed that overexpressed GNG5 elevated Rab5 protein level and vice versa (Figs.', '6B and S10G).', '6C), primary hippocampal neurons (', '6C).', '6D).', '6C and S10C, D).', '6E).', '6F) but did not affect GNG5 mRNA levels (', '6F and S11A).', '6G and S12A, B).', '6H), 293T (', 'tif"">Supplementary \n90% overall nucleotide identity. (D) Single-nucleotide polymorphism (SNP) density map comparing with (red) and (blue) with a 1 kb rolling window.",yes
PMC9123355,Figure_1,oa_package/ac/cd/PMC9123355.tar.gz,['Side-by-side comparison of CT abdomen and pelvis three weeks apart.'],"Figure 1 Side-by-side comparison of CT abdomen and pelvis three weeks apart. (A)Coronal CT scan after percutaneous drainage of the abscess was performed. (B)Coronal CT of the same patient three weeks later showsan abscess with complex features suggesting a mass of the small bowel. The size of the mass is 6x 5.5x 5 cm, similar to the abscess drained during the initial presentation.",yes
PMC8590550,Figure_11,oa_package/76/e5/PMC8590550.tar.gz,[],"Fig. 11 Screening mammography. Full-field digital mammography (FFDM) ( ) of architectural distortion centrally toward the lateral quadrant (arrow) in the right breast which is better appreciated in ( , ) tomosynthesis slice and two-dimensional synthesized mammography (2DSM) images. Note that this is not well identified in the ( ) FFDM mediolateral oblique (MLO) view. However, the area is well seen in the ( , ) tomosynthesis slice (double arrows) and 2DSM in MLO projection. Breast Imaging-Reporting and Data System (BI-RADS) 4A. ( ) Ultrasound sonography (USG) shows hypoechoic area of architectural distortion (AD) with posterior acoustic shadowing (arrow head); BI-RADS 4B. ( ) USG-guided biopsy of the lesion showed ( ) radial scar on histopathological examination (HPE).",yes
PMC10982988,Figure_3,oa_package/9d/c9/PMC10982988.tar.gz,"['7 months) ( 3).', ' 3Time on treatment for all entered NICAM patients, by cKIT mutation exonBar length indicate months on treatment; objective disease progression and death are indicated in the figure.']","Figure2 c- molecular characterization in the NICAM trial The chart shows the individual c- mutation characterization in the 29 trial patients who were enrolled in the molecular profiling. The gene fragment affected by mutations spanned from exons 9 to 17, comprising the immunoglobulin-like-C2 type 5 domain (green), a junction domain (pink), and the protein kinase domain (light blue). Each lollipop anchor corresponds to individual mutation sites (complex mutations are in purple and missense single-nucleotide mutations are in blue), and the height of the lollipop is indicative of the mutation frequency in the trial population.",yes
PMC5705841,Figure_3,oa_package/d4/11/PMC5705841.tar.gz,"[' OX40L on B cells supports plasma cell developmentAll three groups of immunised KO mice showed no difference in the GC B cell population (see online supplementary figure S2B) on day 14 (figure 3A), although during the secondary response, 1 week after the rechallenge, Tnfsf4 / mice showed a smaller proportion of GC B cells (figure 3A).', 'Similarly, no differences were detected in the plasma cell frequency on day 14, but Tnfsf4 / and Tnfsf4fl/fl(CD19) / mice showed a significantly lower percentage of plasma cells on day 42 (figure 3B).', 'OX40L function in GC reaction.', 'We identified the GC TFH population as a subset of CD4+ T cells expressing CXCR5 and high levels of PD-1 (CXCR5+PD-1hi) (figure 3C,F), and in figure 3G the frequencies of splenic GC TFH cells (as a proportion of CD4+ T cells) following immunisation are illustrated.', 'Both Tnfsf4 / and Tnfsf4fl/fl(CD19) / mice showed a reduction in the expression levels of PD1 at both time points, and importantly displayed a greater frequency of CXCR5+ PD1low cells (TFH precursors) in the CD4+ population compared with control mice on day 42 (figure 3D,F).', 'Interestingly, Tnfsf4 / and Tnfsf4fl/fl(CD19) / mice also revealed a reduced number of CXCR5+ cells during the primary (day 14) but not the secondary response (day 42) (figure 3E).', 'Consistent with the immune response data (figure 3), the B6.', 'Our results corroborate this finding; we found that fewer CXCR5+ T cells were generated during the primary response in Tnfsf4 / and Tnfsf4fl/fl(CD19) / mice (figure 3E).', 'In our study, the reduced numbers of TFH cells in Tnfsf4 / and Tnfsf4fl/fl(CD19) / mice were accompanied by an increase in CXCR5+ PD1low cells during the secondary response (figure 3F,G).']","Figure 3 OX40L function in GC reaction. Wild-type controls, , (CD19) and (CD4) mice wereimmunised with NP-CGG in CFA and reimmunised on day 35 with NP-CGG in IFA. Spleens were taken and analysed by FACS either onday 14 orday 42. (A) Frequency of GC B cell (B220+, GL7+, IgD) presented as frequency among the B220+population. (B) Percentage of plasma cells (B220 , CD138 ). (C) Gating of T follicular helper (T ) (CD4+, CXCR5+, PD-1 ) and pre-T follicular helper (pre-T ) (CD4+, CXCR5+, PD-1 ) cells. (D) PD-1 expression level in CD4+cells assessed by FACS. (E) Frequency of CXCR5+cells presented as frequency among the CD4+population. (F) Quantification of pre-GC T and (G) GC-T cells as gated in (C) presented as frequency among the CD4+population. Each symbol represents an individual mouse. Bars indicate the meanSEM, N.S., not significant; *p<0.05, **p<0.01and ***p<0.001 (one-way analysis of variance). CFA, complete Freunds adjuvant; FACS, fluorescence-activated cell sorting; GC, germinal centre; IFA, incomplete Freunds adjuvant; NP-CGG, nitrophenylacetyl-chicken gamma globulin.",yes
PMC5973867,Figure_1,oa_package/88/66/PMC5973867.tar.gz,"['PCR amplification of tumor DNA confirmed Cre-mediated recombination of Pten, Trp53, and Ptpn12 (Supplementary A).', 'Ptpn12 deletion leads to angiosarcoma in mice(A) Schematic for development of triple knockout angiosarcoma mouse model.', 'We found that a small percentage of endothelial cells also express -gal, indicating that CreER is expressed in these cells (Supplementary B).', 'Moreover, we crossed the GFAP-CreER mouse line with Rosa-tdTomato reporter mice and demonstrated Cre-mediated recombination in CD31-positive cells of the skin (Supplementary C).', 'Lastly we used an endothelial cell-specific Cre driver, Tie2-CreER and found that the TKO combination also developed angiosarcomas, albeit with a broader tissue distribution when compared to the GFAP-CreER mice (Supplementary D).']","Figure 1 deletion leads to angiosarcoma in mice ( ) Schematic for development of triple knockout angiosarcoma mouse model. ( ) KaplanMeier curve demonstrating incidence of angiosarcoma in the seven mouse lines generated. KO = 42, KO = 9, KO = 8, KO = 10, KO = 13, KO = 10, triple KO = 30. ( ) Pathology and histology of murine angiosarcomas. Gross dissection of the mouse is shown in the upper panels. Red arrows point to angiosarcomas. Images of the hematoxylin and eosin stained tumors is shown in the lower panels and letter-matched to the gross pictures above (ah). Scale bar in (h) is 50 m and applies to all images in the lower panel. ( ) Histology of human angiosarcoma found in bone compared to the three genotypes of mice that develop angiosarcomas. Scale bar in the top right panel is 100 m and applies to the first row, scale bar in the panel below is 50 m and applies to all remaining pictures.",yes
PMC10572721,Figure_2,oa_package/aa/9f/PMC10572721.tar.gz,"['Three distinct annotations were performed for each slide for the epidermal with junctional component, dermal component, and entire lesion ().', 'Annotation and positive-cell detection preceding the standardized assessment of 5-hmC with Qupath.']","Figure 2 Annotation and positive-cell detection preceding the standardized assessment of 5-hmC with Qupath. Here, ( , ) represents two dysplastic nevi without annotation, ( , ) the annotation of the area of interest (( ): epidermis and dermo-epidermal junction; ( ): dermal component), and ( , ) the positive-cell detection after setting a threshold (the blue cells are considered to be 5-hmC-negative and the red cells to be 5-hmC-positive). Black scale bar = 50 m.",yes
PMC3183048,Figure_7,oa_package/57/95/PMC3183048.tar.gz,"['Spatial reference memory impairment induced by chronic stress is aggravated by the absence of the LPA1 receptorSpatial reference and working memory performanceThree-way repeated-measure ANOVAs performed for the 4 days of spatial learning confirmed the role of the LPA1 receptor in spatial reference memory (A).', 'Importantly, chronic stress treatment differentially altered reference memory in both genotypes, with a more dramatic impairment observed in the stressed nulls (A).', 'In both cases, chronic stress impaired reference memory; interestingly, however, the greater effect of stress on the NULL genotype ( genotype stress interaction) was only significant when considering the first trials of the day (B).', 'Analyses revealed differences in working memory between genotypes, but chronic stress treatment had no effect on this function (C).', 'Both control and stressed NULL mice had poorer working memory than control WT mice on the first training day, although both groups were able to improve in training (C).', 'g007Spatial memory and exploratory and anxiety-like behaviour on the hole-board test.', '000; LSD test shown in A.', '004; LSD test shown in B.', '001; LSD in B.', '002; LSD test shown in C.', '0025522-Strekalova1"" ref-type=""bibr"">[55] (D).', 'NULL mice spent more time in the periphery than WT mice but no effect of stress on thigmotaxis was found (D).', 'LSD tests shown in D.']",10.1371/journal.pone.0025522.g007,yes
PMC6016804,Figure_5,oa_package/f4/43/PMC6016804.tar.gz,"[' shows fast and accurate detection of tumor and characterization of the tumor microenvironment in needle biopsy sections.', 'This slide of biopsies shown in can be imaged and classified in 3 h using the same 6-class technique discussed previously.']","Fig. 5. Rapid triaging of malignant sections using stainless imaging of human breast biopsy samples in feasible times. ( ) Image of needle biopsy sections using absorbance at 1,658 cm with specific malignant regions enlarged for clarity. ( ) The multispectral image was classified using a 6-class model separating cancerous and normal epithelial cells from various collagen-rich stromal types (6S). ( ) Pathologist annotations to the H&E-stained image of a consecutive section demonstrate agreement with the IR-classified image.",yes
PMC7818088,Figure_2,oa_package/02/12/PMC7818088.tar.gz,['Attenuation of acute pathology in the spinal cords of experimental autoimmune encephalomyelitis (EAE) mice injected with proteolipid (PLP) reactive lymph node (LN) T cells from high intensity interval trained mice.'],"Figure 2 Attenuation of acute pathology in the spinal cords of experimental autoimmune encephalomyelitis (EAE) mice injected with proteolipid (PLP)reactive lymph node (LN) T cells from highintensity interval trained mice. Evaluation of demyelination (AC), axonal damage (DF), and inflammation (GO) on cross sections of the spinal cords in EAE mice that were injected with LN T cells from control sedentary (SED transfer [trans] EAE; A, A1, D, D1, G, J, M; n=6) or from highintensity interval trained mice (HIIT trans EAE; B, B1, E, E1, H, K, N; n=6) mice, at 12days post EAE induction. C, F quantification of tissue pathology in spinal cord white matter. I O counts of inflammatory cell types in spinal cord white matter. A, B luxol fast blue (LFB) histochemistry with periodic acid schiff (PAS) counterstaining, A1, B1 LFB histochemistry without PAS counterstaining, dashed lines represent areas of demyelination shown in A and B, respectively; D, E SMI32 immunohistochemistry with hematoxylin counterstaining, D1, E1 SMI32 immunohistochemistry without hematoxylin counterstaining, in brown SMI32+injured axons. LFB staining showed reduction in the area of demyelination in HIITtransfer EAE (B, B1) versus SEDtransfer EAE mice (A, A1, C). SMI32 immunostainnings showed less axonal damage (F) in HIITtransfer EAE (E, E1) than in control SEDtransfer EAE mice (D, D1). In HIIT trans EAE mice there was a reduction in total perivascular immune cell infiltrations (H), in CD3+T cells (K) and nonsignificant reduction in Mac3+macrophages (N) versus SED tansEAE mice (G, J, M, respectively). G, H: arrows indicate perivascular infiltrates; J, K: arrows indicate perivascular CD3+T cells; M, N: arrows indicate Mac3+macrophages. Scale bars=100m. Data are meanSE. * 0.05, *** 0.001.",yes
PMC10565640,Figure_14,oa_package/e0/6b/PMC10565640.tar.gz,[],,yes
PMC10668632,Figure_1,oa_package/50/04/PMC10668632.tar.gz,"['CT scanIn Panel A, an axial view during the arterial phase highlights the inflamed appendix, indicated by the arrow.']","Figure 1 CT scan In Panel A, an axial view during the arterial phase highlights the inflamed appendix, indicated by the arrow. This inflammation is also evident in the coronal view depicted in Panel B. Panels C and D further illustrate the presence of inflamed diverticula, as indicated by the arrow.",yes
PMC9569960,Figure_2,oa_package/5f/c3/PMC9569960.tar.gz,"['The Role of GSK3 -Induced Phosphorylation in Tau Oligomerization and SecretionTo introduce phosphorylation on tau, wild type 0N4R was phosphorylated by transiently overexpressing glycogen synthase kinase 3 (GSK3 ) in different cellular models, SH-SY5Y cells () and HEK293 cells (Supplementary S1).', 'We observed that co-transfection of 0N4R with GSK3 led to an increase in the level of phosphorylation as determined by immunostaining cells with a phospho-tau antibody (pS199, a tau phosphorylation site reported in both healthy and AD brains [26]) (A(i),B).', 'To evaluate the oligomeric content of different cell samples, we carried out immunostaining (A(ii)) against tau oligomeric tau antibody T22 and total tau antibody, Tau5, showing an increase in the level of oligomers formed in the presence of co-expressed GSK3 .', 'The increase of oligomeric tau was also confirmed by sandwich ELISA, in which oligomeric and total tau in cell lysates were measured using T22 and human tau antibody, respectively (C), and a modest increase was observed in GSK3 co-expressing cells.', 'In our study, we evaluated oligomeric tau levels as well as total tau present in the culture supernatant, via indirect ELISA with T22 and HT7 antibodies to further investigate the effects of phosphorylation on tau secretion (D).', 'We find here that co-transfection of GSK3 increased the total tau secretion (D), consistent with the earlier study.', 'We also determined that oligomeric tau secretion (D) into the cell culture medium increased to a modest but statistically significant extent with GSK3 co-transfection, which suggests that phosphorylation contributes to the increased secretion of tau oligomers as well as total extracellular tau.', 'Here, overexpression of GSK3 in SH-SY5Y neuroblastoma cells increased not only the levels of tau phosphorylation (A,B), but also enhanced the cellular immunoreactivity to the tau oligomeric antibodies T22 (A C).', 'The phosphorylation characteristics of PS19 transgenic mice brain extracts.']","Figure 2 The phosphorylation characteristics of PS19 transgenic mice brain extracts. Characterization of SH-SY5Y cells transfected with wild type 0N4R and GSK3 constructs. Representative fluorescent images of tau markers, including pS199 (Green)/Tau-5 (Red)/Hoechst (Blue) ( ( )) and T22(Green)/Tau-5 (Red)/Hoechst (Blue) ( ( )) for SH-SY5Y cells three days post-transfection. Scale bar = 100 m. ( ) Quantitative analysis of fluorescence intensity of pS199 (dark gray hatched bars) and T22 (light gray solid bars), normalized to Tau-5+ intensity as a measure of the total tau expression, for SH-SY5Y cells expressing 0N4R tau with GSK3 co-transfection (GSK3 + 0N4R) or without GSK3 (0N4R), where * represents < 0.05 for = 3. ( ) The oligomeric and total tau were measured by sandwich ELISA of SH-SY5Y cell lysates using T22 and human tau antibody, respectively. * < 0.05 for 0N4R compared with GSK3 + 0N4R ( = 3). ( ) Total tau (open bars) or oligomeric tau (boxes-filled bars) of the conditioned medium were measured by indirect ELISA using HT7 and T22, respectively, to investigate the effects of phosphorylation on tau secretion. (* represents < 0.05 for 0N4R compared with GSK3 + 0N4R ( = 3)).",yes
PMC3023127,Figure_4,oa_package/4a/71/PMC3023127.tar.gz,"['Exposure to antigen led to a significant increase in Foxp3+ cells in the spleens and mLNs (, A C).', '10+Rag2 / cells ( C).', '10 cells produced more IL-2 ( D), whereas IFN- production was minimal irrespective of Il27ra expression in the noninflammatory environment of unchallenged balb/c mice (', 'Importantly, we still observed enhanced Treg conversion when we repeated this experiment under inflammatory conditions in mice that had received WT CD45Rbhi cells 4 wk earlier ( F), suggesting that the inflammatory milieu by itself is insufficient to restrict Foxp3 expression in the absence of IL-27 signaling.', '.', 'Il27ra / effector T cells have a different cytokine secretion profile compared with their WT counterpartsDespite the increased conversion rate of Il27ra / CD45Rbhi cells, the majority of the transferred cells remained Foxp3 .', 'Second, we observed an increase in absolute numbers, not just relative percentages, of Il27ra / Foxp3+ T cells in our OVA-dependent tolerization model ().', 'This difference persisted even in the context of established colitis ( F), and thus represents a cell intrinsic effect that is independent of the inflammation status of the environment into which the cells are transferred.']","Figure 4. (A) Representative Foxp3 staining of WT or (KO) DO11.10 cells after transfer to mice and oral administration of 1.5% OVA in water for 5 d. (B and C) Quantitative analysis of percentage (B) and absolute numbers (C) of Foxp3 cells in the mLNs and spleens of mice receiving WT or DO11.10 T cells and fed 1.5% OVA or control water. (D-E) Intracellular cytokine staining analysis of IL-2 (D) and IFN- (E) in the mLNs from OVA-fed or control mice. (F) Absolute numbers of Foxp3 DO11.10 cells in the mLN of control or OVA-treated mice that had received 3 10 CD45Rb cells 4 wk earlier. Data are from a single experiment representing two individual experiments. *, P < 0.05; ***, P < 0.001.",yes
PMC11278160,Figure_1,oa_package/56/9e/PMC11278160.tar.gz,"['Abdominal CT showed pneumoperitoneum with a large amount of fluid in the pelvis and abdomen, indicating hollow viscus rupture ().', 'C215007000\nAbdominal CT shows sigmoid colon perforation (arrow).']",Figure 1 Abdominal CT shows sigmoid colon perforation (arrow).,yes
PMC8743000,Figure_3,oa_package/f7/49/PMC8743000.tar.gz,"['At the 1-month time-point, CD11b + (A), CD68+ (', '3B) and CD206+ (C) macrophages were broadly dispersed throughout the muscles of WT/mdx mice.', 'In contrast, CD11b + (D) and CD68+ (', 'CD206+ macrophages also exhibited a clumped population dispersion in inflammatory lesions of LIF/mdx mice (F), although the effect was not as apparent as it was for CD68+ macrophages.', 'The transgene did not affect macrophage dispersion at the 3-month (G-L) or 12-month (data not shown) time-points.', '\n\nThe LIF/transgene inhibits dispersal of macrophages in dystrophic muscles.', '5B) mice showed albumin+ fiber clusters that resembled CD68+ macrophage clusters at the same time-point ().', 'Cross-sections from 1-month-old WT/mdx (A) and LIF/mdx (B) muscles labeled with anti-albumin show injured fiber clusters that resemble CD68+ macrophage dispersion (B and3E).']","Figure 3 The LIF/transgene inhibits dispersal of macrophages in dystrophic muscles. Muscle cross-sections of 1- and 3-month-old TA muscles from WT/ and LIF/ mice were immunolabeled for macrophage markers to assess immune cell localization. Labeling with anti-CD11b , anti-CD68 and anti-CD206 shows that transgene expression modifies macrophage dispersion in LIF/ mice at 1-month, but not at 3-months of age. Scale bars=500m.",yes
PMC3843925,Figure_1,oa_package/e6/7e/PMC3843925.tar.gz,"['References1SpencerJMAmonetteRATumors with smooth muscle differentiationDermatol Surg19962276176888745232KatoYHoriJTaniguchiNSolitary genital leiomyoma of the tunica dartos: a case report and review of the literature in Japan (in Japanese)Hinyokika Kiyo200551699701162856273PhilipJManikandanRVishwanathanPSymplastic scrotal leiomyoma: a case reportJ Med Case Reports200822954Su rez-Pe arandaJMVieitesBEvgenyevaEMale genital leiomyomas showing androgen receptor expressionJ Cutan Pathol200734946949180014205ChiongETanKBSiewEUncommon benign intrascrotal tumoursAnn Acad Med Singapore200433351355151757786SiegalGPGaffeyTASolitary leiomyomas arising from the tunica dartos scrotiJ Urol197611669719453607SherwaniRKRahmanKAkhtarKLeiomyoma of scrotumIndian J Pathol Microbiol200851727318417864Pink, hyperplastic plaques, with an uneven surface, basal infiltration, and ill-defined, sheet depigmentation in the center.']","Fig. 1 Pink, hyperplastic plaques, with an uneven surface, basal infiltration, and ill-defined, sheet depigmentation in the center.",yes
PMC4993389,Figure_10,oa_package/bf/f5/PMC4993389.tar.gz,[],10.1371/journal.pntd.0004935.g010,yes
PMC9005588,Figure_6,oa_package/04/4d/PMC9005588.tar.gz,"['As shown in , the injection of PCPDX tumor cells resulted in metastases to the lung.', '.']","Figure 6. H&E of mouse lung tissue with metastatic tumor foci. H&E slides were assessed at 4x magnification by a genitourinary pathologist for tumor foci, indicated by an asterisk.",yes
PMC11634550,Figure_3,oa_package/7e/8d/PMC11634550.tar.gz,['Histopathology photomicrograph I (200 magnification)Nests of cells with clear cytoplasm and small nuclei are separated by delicate septae containing thin-walled blood vessels (indicated by green stars).'],Figure 3 Histopathology photomicrograph I (200 magnification) Nests of cells with clear cytoplasm and small nuclei are separated by delicate septae containing thin-walled blood vessels (indicated by green stars). An area of necrosis (indicated by a red star) is surrounded by cells with eosinophilic cytoplasm.,yes
PMC5556803,Figure_4,oa_package/39/67/PMC5556803.tar.gz,"['The postoperative orbital volume was measured based on the orbital and implant borders, and the volumetric ratio of the uninjured orbital cavity to the injured orbital cavity was used to reduce the gap in the volume among patients as well as the errors by the uninjured orbital volume () [13].', 'Preoperative and postoperative 3D modeling and orbital volume.']","Fig. 4 Preoperative and postoperative 3D modeling and orbital volume. (A) Preoperative 3D modeling; right inferomedial blowout fracture, (B) Postoperative 3D modeling with the applied implant, (C) Preoperative 3D modeling of the orbital volume, (D) postoperative 3D modeling of the orbital volume; reduced orbital volume on the inferomedial area.",yes
PMC6580335,Figure_4,oa_package/4a/36/PMC6580335.tar.gz,['Intra-operative appearance of the tumour mass.'],"Figure 4 Intra-operative appearance of the tumour mass. A: The right parotid gland part of the tumour was separated; B: The basicranial part of the tumour was removed; C: The right mastoid process and cerebellar dura mater were resected. The mass was clearly lobular in shape, and the mastoid was partially destroyed. (April 2017)",yes
PMC4082918,Figure_5,oa_package/75/cf/PMC4082918.tar.gz,"['004""/>Small dark relatively uniform nuclei with indistinct cytoplasm.']",Figure 5 Small dark relatively uniform nuclei with indistinct cytoplasm.,yes
PMC10923822,Figure_3,oa_package/48/aa/PMC10923822.tar.gz,"['3 and the set of labeled instances consists of the JSRT data set, while the set of unlabeled instances consists of the PadChest data set.', '3.', 'Semi-supervised segmentation results (CXR Ulm).', 'The color blue is specific to the lungs while the color red refers to the heart.', '3).', '3).']","Figure 3 Semi-supervised segmentation results (CXR Ulm). The top row consists of the segmentation models output (filled areas) and the ground truth (contours). At the bottom, the exact same set of images is displayed as above, this time however uniquely with the segmentation models output and the computed bounding boxes around the areas of interest.",yes
PMC11612450,Figure_3,oa_package/bf/53/PMC11612450.tar.gz,"[' 3A).', 'LncMtDloop is required for the recruitment of TFAM and mitochondrial transcription initiation.', 'The mitochondrial genome homeostasis is essential for mitochondrial function, and its deleterious mutations are implicated in metabolic disorders, aging, and neurodegenerative disorders (Antonyova et al, 2020; Sanchez-Contreras et al, 2023).', ' 3B) (Xavier and Martinou, 2021).', ' 3C), and further experimental analyses with western blot, RIP, and RISH-ICF confirmed such prediction (', ' 3D G).', ' 3H,I).', ' 3J).', ' 3K,L).', ' 3M; Appendix ', ' 3N,O).', 'zip"">Source data \nCase 2: anteroposterior left foot radiograph showing a lytic and destructive mass centered on the 4th metatarsal, with destructive extension to the 3rd metatarsal and adjacent cuneiform bones.']","Figure 6 Case 2: anteroposterior left foot radiograph showing a lytic and destructive mass centered on the 4th metatarsal, with destructive extension to the 3rd metatarsal and adjacent cuneiform bones. Punctate calcifications consistent with foci of mineralization can be seen throughout the lesion.",yes
PMC10331499,Figure_2,oa_package/ed/1c/PMC10331499.tar.gz,"['A postoperative CT was performed which demonstrates the bony removal after reaming and endoscopic drilling ().', 'Post-operative CT myelogram.']","Figure 2 Post-operative CT myelogram. (A) Sagittal CT reconstruction and (B) axial CT demonstrating the foraminotomy (open arrows) by the reamers and the endoscopic drill. CT, computerized tomography.",yes
PMC10747589,Figure_4,oa_package/fc/8e/PMC10747589.tar.gz,"['In the spleen, lesions comprised degeneration and loss of mononuclear cells in both the red pulp and the white pulp and prominent tingible body macrophages (E,F).', 'Staining was most prominent in the liver (C,D) and spleen (G,H).', 'Microscopic lesions and viral RNA staining of animals infected with mammalian cell- (left) and insect cell-grown (right) RVF virus in the liver and spleen.']","Figure 4 Microscopic lesions and viral RNA staining of animals infected with mammalian cell- ( ) and insect cell-grown ( ) RVF virus in the liver and spleen. Liver; multifocal hepatocyte degeneration and necrosis denoted by arrows ( , ) and viral RNA staining ( , ). Spleen; variable loss of mononuclear cells in the red and white pulp ( , ) and viral RNA staining ( , ). Inset, higher magnification images of the area highlighted by the smaller box in the main panel. HE, ISH. Scale bar denotes 100 m for main panel and 50 m for inset panels.",yes
PMC10406435,Figure_17,oa_package/9a/8c/PMC10406435.tar.gz,[],"Figure 11. CAF-382 (B1) acutely reduces the expression of long-term potentiation (LTP) mediated by AMPA-type glutamate receptors in CA1 hippocampus. Hippocampal slices were initially incubated in CAF-382 ( ) (100 nM) for at least 60 min prior to recording. Peak negative current of AMPA-type glutamate receptor-mediated synaptic responses were normalized to initial baseline post break-in (time = 0), expressed as 100% (dotted line); mean SEM shown. Following baseline, LTP was induced by a pairing protocol. Compared to interleaved control slices, CAF-382 ( ) significantly reduced LTP at 2731 min post break-in (control: 197.5 8.4 n = 14 slices, 14 rats; CAF-382 ( ): 123.14 9.0, n = 1213 slices, 13 rats; p<0.001, two-way ANOVA, HolmSidak post hoc). Sample traces for control (black filled circle) and CAF-382 ( , red filled circle) before (1, solid trace) and after LTP (2, dotted trace) are shown to the right. Error bars are SE.",yes
PMC3726683,Figure_5,oa_package/75/54/PMC3726683.tar.gz,['g005Transmission electron micrographs of ARPE-19 cells exposed to AICAR or/and MG-132 and autophagic vesicles quantification.'],10.1371/journal.pone.0069563.g005,yes
PMC10348775,Figure_7,oa_package/a8/b5/PMC10348775.tar.gz,"['We found that the expression levels of mtDNA-encoded ETC complex subunits were aberrantly downregulated, presenting translational imperfections and partially impeding the assembly and stability of RSCs along with cristae in HCM pathological cybrids (A).', 'The relative reaction activity of complexes I, III, IV, and V in the HCM cybrids represented a considerable collapse of these values compared with those of controls (, B E).', 'To further investigate the effect of DNJ on mitochondrial OXPHOS activity, the oxygen consumption rates (OCR) of cybrids were analyzed (F).', 'DNJ benefits mitochondrial function in HCM cybrids.']","Figure 7 DNJ benefits mitochondrial function in HCM cybrids. ( ) Western blotting and quantification analysis of respiratory electron transport chain complex subunits (MT-ND5, MT-COX2, MT-ATP8, and MT-CYB). TOM20 is shown as a loading control. ( ) The activities of OXPHOS complexes were investigated using enzymatic assays on complex I, III, IV, and V. = 5 biologically independent experiments. Values represent the mean SEM. 1-way ANOVA followed by Tukeys test. * < 0.05, ** < 0.01, *** < 0.001. ( ) Oxygen consumption rate (OCR) was measured using a seahorse analyzer. = 3 biologically independent experiments. Data are representative of 3 independent experiments. Values represent the mean SEM.",yes
PMC4992006,Figure_3,oa_package/67/48/PMC4992006.tar.gz,['Folfox regimen with Irinotecan and Bevacizumab has been started and the patient is still undergoing treatment ().'],"Fig. 3 A. Imaging of the patient. PET CT. Multiple osteolytic metastases to the vertebral bodies and to the left omoplate, associated with a fracture. B. Abdominal CT scan. A mass with a possible abscess is visualized in the ileo-caecal zone.",yes
PMC6906178,Figure_1,oa_package/24/f2/PMC6906178.tar.gz,"['The structure of the GluN1/GluN2B NMDA receptor ion channel has been solved by X-ray crystallography at 4 resolution (A; Karakas and Furukawa, 2014).', ', 2012), which comply with the force-from-lipids paradigm of MS channel gating (B; Martinac et al.', 'The bonus of these findings is that local curvature on the scale of tens of nanometers can be influenced by a host of proteins, lipid types and unilateral insertion of physiologically active amphipathic molecules, such as arachidonic or lysophosphatidic acid (; Zimmerberg and Kozlov, 2006; Kloda et al.', 'Nikolaev for designing as well as for his comments and proofreading the manuscript.']","FIGURE 1 Overall structure and activation mechanism of N-methyl-D-aspartate receptor (NMDAR) ion channel. 3D structure of heterotetrameric NMDAR channel consisting of two GluN1a and two GluN2B subunits [adapted from ]. Glycine and glutamate binding sites are indicated on the GluN1a and GluN2B subunits, respectively. Mg binding site is represented by a dark blue circle. CTD refers to cytoplasmic domain. Activation of NMDAR channel by insertion of an amphipathic molecule (e.g., arachidonic acid (AA) indicated by light blue rods) into the extracellular leaflet of the membrane bilayer ( ). One-sided insertion of AA is curving the bilayer, which changes the lateral pressure in the bilayer (black profile inset) causing the channel to open ( ).",yes
PMC8160561,Figure_29,oa_package/9c/f7/PMC8160561.tar.gz,[],"Fig.29 Stills disease: 34-year-old male patient with no prior significant medical history now presented with fever, rash, polyarthralgia, and diffuse abdominal pain. Physical examination revealed severe splenomegaly. Initial US evaluation detected multiple hypoechoic parenchymal nodules in the spleen (not shown). Subsequent MRI exam showed multiple T2-hypointense splenic nodules (arrows) with associated splenomegaly. Also, note was made of a trace amount of intraperitoneal fluid (arrowhead). Extensive diagnostic workup diagnosed Stills disease",yes
PMC9600253,Figure_1,oa_package/31/77/PMC9600253.tar.gz,"['0 cm solid right ovarian mass (A).', '4 cm endometrial mass with soft tissue density (B) and an 8.', '6 cm solid and cystic left ovarian mass (C).', 'Abdominopelvic CT revealed a 1 cm endometrial mass that appeared to be invading the superficial myometrium (D).', '8 cm (E).', 'Abdominopelvic MRI revealed no visible neoplastic lesions in the cervix, endometrium, lymph node, and abdominopelvic peritoneum (F).', '000000000000055330550485MRI, CT, and gross findings.']","Figure 1 MRI, CT, and gross findings. ( ) Case 1: T2-weighted coronal MR image reveals solid and cystic bilateral ovarian masses (blue arrows). ( , ) Case 2: T1-weighted Dixon sagittal MR images reveal ( ) 3.4 cm endometrial mass (yellow arrow) and ( ) 8.6 cm solid and cystic left ovarian mass (purple arrow). ( ) Case 3: Contrast-enhanced CT image reveals a 1 cm endometrial mass (green arrow) and hematometra. ( ) Case 3: An irregularly elevated mass is noted in the endometrium. The endometrial cavity is distended with blood. The endocervix (white arrow) appears unremarkable. ( ) Case 4: T2-weighted sagittal MR image reveals no identifiable lesion in the endometrium.",yes
PMC6224548,Figure_6,oa_package/45/14/PMC6224548.tar.gz,"[' 6A).', ' 6B).', 'The reduction of Treg cells in ongoing PCM of DEREG mice increases the number of pulmonary Th1 and Th17 cells but reduces the presence of Th2 lymphocytes.', 'Treg cells depletion in ongoing PCM increases the Th1/Th17 cytokines and reduces Treg cells-associated cytokinesLung cell supernatants from DT and PBS treated infected DEREG mice were obtained at weeks 6 and 10 of infection and pro- and anti-inflammatory cytokines measured by ELISA (']","Figure 6 The reduction of Treg cells in ongoing PCM of DEREG mice increases the number of pulmonary Th1 and Th17 cells but reduces the presence of Th2 lymphocytes. The phenotypic analysis of lung infiltrating lymphocytes from DT and PBS (control) treated DEREG mice was performed at weeks 6 and 10 of infection. The lung cells were obtained as described in Material and Methods and labeled with antibodies conjugated to different fluorochromes. The lung infiltrating leukocytes were gated by FSC/SSC analysis. The cells were gated for CD4 ( ) or CD8 ( ) expression and then for intracellular expression of IFN-, IL-4 and IL-17. One hundred thousand cells were acquired on FACS CANTO II and subsequently analyzed by FlowJo software. Data are expressed as meansSEM of three independent experiments using 5 mice per group (* <0.05, ** <0.01 and *** <0.001).",yes
PMC3170270,Figure_10,oa_package/f3/8b/PMC3170270.tar.gz,[],"Figure 10 ."" 2-year postoperative correction anteroposterior and lateral X-rays of the patient in Figure 9. A 3-3-3 pattern of instrumentation was utilized. The patient shows good correction, is well balanced in the coronal plane, the shoulders are level, and the dorsolumbar kyphosis has been restored to normal.",yes
PMC6077576,Figure_1,oa_package/89/b9/PMC6077576.tar.gz,['Thrombosis in newborn infantsArchivos argentinos de pediatria2016114Comite Nacional de Hematologia OyMT15916627079395Cross-sectional images of the fetal pelvis: (a) at 20 weeks of gestation showing with color Doppler the two umbilical arteries around the fetal bladder and (b) at 29 weeks of gestation with only one umbilical artery.'],Figure 1 Cross-sectional images of the fetal pelvis: (a) at 20 weeks of gestation showing with color Doppler the two umbilical arteries around the fetal bladder and (b) at 29 weeks of gestation with only one umbilical artery.,yes
PMC10300792,Figure_35,oa_package/2d/ef/PMC10300792.tar.gz,[],"Figure 34 Diphtrie cutane Lgende: A. Patient du Maroni de 46 ans prsentant depuis 5 jours des lsions multiples des deux jambes ulcres, fibrineuses et inflammatoires de 1 1,5 cm de diamtre; B. Lsions cutanes multiples et diffuses sur l'ensemble du corps (oreille, visage, aine) associes des adnopathies sensibles, apparues depuis 3 jours chez un enfant du Maroni de 3 ans (crdit photos: M. Gaillet) Cutaneous diphtheria A. 46-year-old patient from Maroni with multiple ulcerated, fibrinous and inflammatory lesions on both legs, 1 to 1.5 cm in diameter, since 5 days; B. Multiple diffuse skin lesions all over the body (ear, face, groin) associated with tender adenopathies, which appeared 3 days before in a 3-year-old child from Maroni (photo credits: M. Gaillet)",yes
PMC7594223,Figure_1,oa_package/c6/33/PMC7594223.tar.gz,"['Vitreous opacity in both eyes prevented detailed fundus visualization, but the\noptic discs in both eyes appeared atrophic (B).', '.', '1177_2324709620966843-fig1""/>She underwent vitrectomy combined with cataract surgery at first in the right eye\nand later in the left eye.', 'On cytological examinations, the vitreous aspirates\ncontained medium-sized cells with aberrant nuclei (A).', 'Immunostaining revealed that\nthese cells were positive for CD3, indicative of T-cells (C).', 'Cytology of vitreous aspirates demonstrated\nunequivocally ATLL cells (\n1A).']","Figure 1. Case 1. A 67-year-old woman with vitreous opacity and atrophic optic discin the right eye (B, left panel) and the left eye (B, right panel).Vitreous cytology of vitrectomy fluid in both eyes demonstratesmedium-sized cells with aberrant nuclei in hematoxylin-eosin stain (A),which is positive for CD3 (C), indicative of T-cells. Bar = 50 m.",yes
PMC11408156,Figure_7,oa_package/67/06/PMC11408156.tar.gz,"['7Tirzepatide improves novel object recognition task performance in 6-month-old male 5XFAD miceLocomotor activity, expressed as total distance travelled and time spent in the center area during the open field test, were not consistently different in 6-month-old male or female 5XFAD mice treated with semaglutide or tirzepatide ( 7A D).', 'On novel object recognition testing, male tirzepatide-treated, but not semaglutide-treated, 5XFAD mice exhibited longer exploration times and higher discrimination indices for the novel object, indicating improved memory ( 7E H).', 'Neither semaglutide nor tirzepatide treatment impacted tasks assessed using the Morris water maze, including latency time, latency to target quadrant, or number of site crossings in target quadrant ( 7IN and Supplementary 7I-L).', ' 7Memory performance tests in 6-month-old 5XFAD mice.']","Figure6 (A, B) Representative images of (A) the cerebral cortex (Cortex) and (B) the hippocampus (Hippo) and subiculum (Sub) brain regions of 5XFAD mice. Right panels in (A) represent magnification of the area of the cerebral cortex highlighted in the left panels. Scale bars in (A) represent 100m (left panels) and 10m (right panels); scale bar in (B) represents 50m. Quantification of plaque intensity in the cerebral cortex (C, D), hippocampus (E, F) and subiculum (G, H) after Thioflavin S staining in 6-month-old 5XFAD and WT-matched female () and male () mice treated with semaglutide (25nmol/kg), tirzepatide (10nmol/kg), or vehicle (saline). Data are represented as meansSD. n=511 in each group of vehicle (Veh)-, semaglutide (Sema)-, or tirzepatide (TZP)-treated mice.",yes
PMC6599347,Figure_1,oa_package/52/3d/PMC6599347.tar.gz,"[' 1a for simplified pathway).', 'Tau rTg4510 mice show significant polyamine dysregulation.', 'Physical and emotional stimuli are known to elicit a polyamine stress response (PSR), resulting in altered central polyamine homeostasis [30 32].', ' 1b, c), and tangles (not shown) in the forebrain and hippocampus but also develop many components of tauopathies including inflammation and atrophy [43, 44, 59].', ' 1d h), which was associated with polyamine dysregulation at the level of enzymatic and protein control (', ' 1i, j).', ' 1i, j), relative to non-transgenic controls.', ' 1d h) as well as its ability to enhance the spreading of tau pathology [61], assemble more rapidly and more extensively into tau filaments than wild-type tau in vitro [10], and produce cognitive impairment [62].', 'tif"">Additional file 1:Supplemental figure 1.']","Fig. 1 Tau rTg4510 mice show significant polyamine dysregulation. Simplified polyamine pathway. Ornithine decarboxylase antizyme 1 (OAZ1), ornithine decarboxylase (ODC), spermidine synthase (SRM), spermidine synthase (SMS), spermine oxidase (SMOX), spermidine/spermine- -acetyltransferase (SSAT), polyamine oxidase (PAOX), polyamine-modulated factor 1 (PMF1), polyamine modulated factor binding protein 1 (PMFBP1). , Representative images and quantification of western blot analysis of 12-month-old nTg and rTg4510 ( =5) hippocampal tau neuropathology (pSer199/202: (4 )=7.490, =.002; pSer356: (4 )=4.588, =.010; and total tau (H150): (4 )=4.201, =.014), followed by quantification ( =5). Independent sample test with Levenes test for equality of variance correction, * <.05. Data is represented by meansS.E.M. Representative images and quantification of immunohistochemical and western blot analysis of 14-month-old nTg and rTg4510 ( =24) cortical and hippocampal C-terminally truncated tau neuropathology (tau D421). Data is represented by means S.E.M. Representative images and quantification of western blot analysis of 12-month-old nTg and rTg4510 ( =45) polyamine dysregulation (ODC: (4.843 )=4.320, =.008; SRM: (5.347 )=3.756, =.012; SMS: (8)=4.412, =.003; SSAT: (7)=2.849, =.025; SMOX: (4.399 )=2.958, =.037; PMF1: (4.113 )=2.690, =.053; PMFBP1: (8)=3.850, =.005). Independent sample test with Levenes test for equality of variance correction, * <.05. Data is represented by meansS.E.M.",yes
PMC10638707,Figure_2,oa_package/3a/79/PMC10638707.tar.gz,"[' 2.', 'Representative CT images for the quantitation.', 'a PAT (dark green); b pectoralis muscle (purple) and SAT of the chest wall (light green)Blood samples were centrifuged at 3000 rpm for 10 min, and serum was collected for measurement of the following cytokine levels using standard enzyme-linked immunosorbent assay techniques: human IL-1 (AiFang Biology, catalog number AF-03336H1), IL-4 (AiFang Biology, catalog number AF-03206H1), IL-6 (AiFang Biology, catalog number AF-03204H1), interferon- (IFN- , AiFang Biology, catalog number AF-03188H1), C-reactive protein (CRP, AiFang Biology, catalog number AF-03290H1), and TGF- 1 (AiFang Biology, catalog number AF-03245H1).']",Fig. 2 Representative CT images for the quantitation. PAT (dark green); pectoralis muscle (purple) and SAT of the chest wall (light green),yes
PMC5347934,Figure_4,oa_package/34/ef/PMC5347934.tar.gz,['Double sided chair side reference chart designed to aid in the differential diagnosis of AMD phenotypes using IR imaging: (a) Intermediate AMD and scattered drusen of various sizes.'],"Figure 4 Doublesided chair side reference chart designed to aid in the differential diagnosis of phenotypes using imaging: (a) Intermediate and scattered drusen of various sizes. The small and medium sized drusen (<125m) were predominantly hyperreflective, while the effect of large drusen (125m) was more inconsistent (both hyper and hyporeflective). (b) Reticular pseudodrusen ( ), which typically present as a reticular pattern of small, yellowwhite, round lesions using colour retinal photography and as groups of hyporeflective lesions using imaging. Larger lesions may be accompanied by a halolike appearance, also known as a target aspect, with increased centrally surrounded by a halo of decreased reflectivity. (c,d) Geographic atrophy secondary to ; the atrophic regions appear as welldemarcated, bright zones of hyperreflectivity, which relates to the absence of . The presentation may be (c) unilobular involving the fovea or (d) multilobular, extrafoveal and/or associated with drusen of variable sizes. Note that the atrophic areas may be more apparent using imaging than retinal photography. (e,f) Neovascular ; the images reveal (e) sharpbordered, oval lesions and speckled hyperreflectivity that suggest occult , and (f) a dark core bordered by a hyperreflective ring or halo consistent with the typical appearance in classic . Cases were chosen based on the consistency of the image to previous descriptions provided in the literature. *A size criterion of 300m was adopted by Xu . Historical characterisations of atrophic lesions in applied a 175m cut off to retinal photography observing the difficulties in detecting choroidal vessels and edges of atrophic areas in smaller regions.",yes
PMC10507381,Figure_2,oa_package/ec/21/PMC10507381.tar.gz,"['95 ( 2A).', ' 2Evaluation of the prediction performance for the biomarkers MSI, BRAF, and KRAS in single cohort and large-scale multi-centric experimentsExperimental results for MSI-high (A C), BRAF (D,E), and KRAS (F,G) prediction.', 'Second, we trained our model on all cohorts of CRC resections except YCR-BCIP and evaluated it on the external cohort YCR-BCIP ( 2B).', '9% MSI-high samples on average across all cohorts ( 2C).', '09 for training on the NLCS and testing on the TCGA cohort ( 2).', '87, respectively ( 2D).', '88, almost reaching clinical-grade performance ( 2E).', 'We observed similar results regarding the generalization when investigating KRAS as a target (s 2F and 2G), with an AUROC of 0.']","Figure1 Workflow overview with pre-processing and model architecture and cohort overview (A) The data pre-processing pipeline with the steps whole slide image (WSI) digitization, tissue segmentation, WSI tessellation into patches, and stain augmentation, (B)the model architecture including the pre-trained feature extractor CTransPath and our transformer-based aggregation module, and (C) details of the transformer layer architecture. (D) Overview of the 16 cohorts of CRC resections and biopsies with MSI/dMMR status, which were used in this study and the subsets of six cohorts with (E) and (F) ground truth data, respectively.",yes
PMC4871380,Figure_1,oa_package/4d/41/PMC4871380.tar.gz,"[""A) The first layer of reconstruction (approximating the musculofascial plate posterior to the urethra (green line region) and the Denonvillier's fascia posterior to the bladder (blue line region) and the bladder musculature.""]","Figure 1 A) The first layer of reconstruction (approximating the musculofascial plate posterior to the urethra (green line region) and the Denonvillier's fascia posterior to the bladder (blue line region) and the bladder musculature. u, urethra, LA, levator ani, B, bladder neck, (the yellow region). B) Illustration shows a separate u type of suture that is used for the first layer reconstruction. C) A 3/0 monocryl absorbable suture is used to approximate the remaining arcus tendineus and distal triangular plate anterior to the urethra (including the residual of the endopelvic fascia and puboprostatic ligaments, rhabdosphincter, dorsal venous complex) to the bladder neck. D) Illustration shows the anterior reconstruction.",yes
PMC5214942,Figure_6,oa_package/85/f3/PMC5214942.tar.gz,"['Contrary to naive Aim2 / IECs, where the expression of Reg3b and Reg3g was impaired, we observed that both AMPs were significantly elevated in Aim2 / IECs at both time points during DSS-induced colitis, whereas WT IECs were not significantly altered (a).', 'However, Aim2 / IECs showed reduced Defcr5 expression compared with WT IECs 5 days post DSS administration, but they normalized during repair at day 14 (b).', 'Bd14 expression was elevated during acute colitis and was further elevated in Aim2 / IECs, but it was downregulated during repair 14 days after DSS administration (c).', 'Il22bp expression is downregulated in DCs during acute colitis,63 and we observed a comparable Il22bp expression pattern in IECs during acute colitis (d).', 'Consistent with the increased Il22bp expression during the resolution phase at day 14 after DSS administration, IL-22 was reduced in Aim2 / mice (e).', 'In addition, TNF- , IL-1 and IFN- levels were also all reduced in Aim2 / mice 14 days after DSS administration (e), suggesting a more rapid resolution of intestinal inflammation in Aim2 / mice.', 'During this short time, IL-18 could not have modulated IL-22 through the regulation of IL-22BP, as shown in d.', 'org/1999/xlink"" xlink:href=""cmi201635f5""/>Loss of Aim2 promotes Reg3 / production and improved recovery from colitis.']","Figure 6 Loss of promotes Reg3/ production and improved recovery from colitis. Colitis was induced in WT and mice with 3% DSS. ( ) IECs were isolated at days 0, 5 and 14 after DSS administration and were analyzed by real-time PCR for the expression of ( ) and , ( ) and ( ) and ( ) ( =716). The data are presented as fold induction compared with naive WT mice shown in . ( ) Spontaneous release of IL-22, TNF-, IL-1 and IFN- was measured by ELISA from cleared culture supernatants obtained after culture for 17h of colonic tissue explants obtained 14 days after DSS administration. ( =45), and error bars represent s.e.m.; * <0.05.",yes
PMC11529445,Figure_5,oa_package/fd/c9/PMC11529445.tar.gz,"[' 5A).', ' 5B, C).', ' 5D).', ' 5E-K).', ' 5E-G).', ' 5H).', ' 5I-K).', ' 5K).', ' 5L, M).', ' 5N).', ' 5O-R).', '\nABT-263 treatment from disease onset reduces severity, inflammation, promotes neuronal survival.', '001\nConclusionThe data presented here demonstrate that in EAE, microglia develop a senescent phenotype based on transcriptomics and IHC, and this subpopulation of microglia and potentially macrophages may be depleted through senolytic treatment.']","Fig. 5 ABT-263 treatment from disease onset reduces severity, inflammation, promotes neuronal survival. Experimental design demonstrating induction of EAE followed by ABT-263 treatment at symptom onset, optomotor testing at peak disease, and tissue collection at 18 days post induction (d.p.i.). EAE score from day starting ABT-263 or vehicle treatment, data are combined numbers from two separate EAE cohorts. Bars=SEM. Two-way Anova with Sidaks multiple comparisons test with adjusted pvalues, * < 0.05, ** < 0.01. Weight calculated as difference from baseline of day before symptom onset/treatment start (day 0). Bars=SEM. Two-way Anova with Sidaks multiple comparisons test with adjusted pvalues, * < 0.05. Optomotor response data for right and left eyes of EAE mice at peak treated with either vehicle or ABT-263 for at least 3 days prior to evaluation. 3 stripe sizes were tested (0.3 c/d, 0.35 c/d, and 0.4 c/d) and data were binned into two groups no response (n.r.)/0.3 and 0.35/0.4. Number in pie slices represents % of responses in that category. Binomial test for observed versus expected distribution. , Thresholded images of EAE optic nerves from vehicle treated (E) or ABT-263 treated (F) mice labeled with IBA1 (red) and Hoechst (white). Scale bar =250m. Quantification of the percent area of optic nerve positive for Hoescht dye from thresholded images. Error bars=SD, two-tailed unpaired t-test. Quantification of the percent area of optic nerve positive for IBA1 immunostaining from thresholded images. Error bars=SD. Two-tailed unpaired t-test. , hresholded images of EAE optic nerves from vehicle treated (I) or ABT-263 treated (J) mice labeled with fluoromyelin (FM-red) and Hoechst (white). Scale bar =250m. Quantification of the percent area of optic nerve positive for Fluoromyelin dye from thresholded images. Error bars=SD, one-tailed Welchs t-test. , Representative images of flatmount retinas labeled with RGC marker BRN3A in vehicle treated (L) and ABT-263 treated (M) EAE mice. Scale=50m. Quantification of BRN3A positive cells per area in retinas from vehicle or ABT-263 treated mice. Bars=SD. Two-tailed unpaired t-test. , Representative images of EAE optic nerves labeled with IBA1 and BCL-xL from control and ABT-263 treated animals. Scale bar =200m. Fluorescence intensity of BCL-xL in IBA1+area. Fluorescence intensity of BCL-xL in the entire optic nerve area. Error bars=SD, two-tailed unpaired t-test performed. For E-R, * = pval<0.05, ** = pval<0.01, and *** = pval<0.001",yes
PMC7869933,Figure_4,oa_package/85/67/PMC7869933.tar.gz,['Correlation of NPASS values with donor CIT.'],Figure 4 Correlation of NPASS values with donor CIT. Scores in head (black) and tail (white) region of each DBD (A) and DCD (B) pancreas for each NPASS parameter are plotted against CIT for that organ.,yes
PMC9850878,Figure_3,oa_package/25/1d/PMC9850878.tar.gz,"['In three patients, procedures done were not specified [ and Table 4].', 'Huge gastric fundal gastrointestinal stromal tumors with local infiltration of the adjacent anterolateral abdominal wall and multiple liver metastasisTable 4Surgery performed according to the site of the tumour(n = 28) and post-operative complications(n = 8)SiteNumber of patientsMain procedure performedGastric16Open sleeve/wedge tumor resectionGastric2Open distal gastrectomyGastric2Laparoscopic wedge resectionSmall bowel3Segmental resectionColon1Left hemicolectomyColon1Segmental transverse colectomyAbdominal mass3Unspecified tumor resection\n\n\nProcedure-related complication per patient\n\nComplication\n\nOutcome\n\n\nOpen gastric wedge excisionRecurrent metastatic tumorAdjuvant imatinib + tumor re-excisionOpen gastric wedge excisionRecurrent obstructing metastatic tumor with peritoneal seedlingsDied of postoperative enterocutaneous fistulaOpen gastric wedge excisionWound dehiscence and recurrent abdominal painDeath?', 'Regardless of the presenting symptoms, an abdominal CT finding of local invasion of adjacent structures [] in 45.']",Figure 3 Huge gastric fundal gastrointestinal stromal tumors with local infiltration of the adjacent anterolateral abdominal wall and multiple liver metastasis,yes
PMC7654429,Figure_3,oa_package/84/15/PMC7654429.tar.gz,['phosphorylation of NF B is involved in the formation of osteoclasts regulated by low dose IL 2.'],"Figure 3 phosphorylation of NFB is involved in the formation of osteoclasts regulated by lowdose IL2. MCSF (30ng/ml) and RANKL (50ng/ml) were used to stimulate CD11b+ from bone marrow and cultured with PBS and IL2 (5 IU/ml) for 4 days, then trap staining was performed. (A) Represented Western blot figure for the levels of NFb p50 and p65 (B) relative level of NFb p50 and p65 activation were determined by Western blot. ** .01, *** .001. All data were from the mean of three independent experiments. DMSO, dimethyl sulfoxide; IL, interleukin; NFB, nuclear factorB; RANKL,receptor activator of nuclear factorB ligand",yes
PMC10565640,Figure_9,oa_package/e0/6b/PMC10565640.tar.gz,[],Figure EV4 Differential gene expression in the cochlea by RNA sequencing and qRTPCR,yes
PMC7541836,Figure_11,oa_package/f7/7a/PMC7541836.tar.gz,[],"FIGURE 11 Fenofibrate exerts IL-10 dependent and IL-10 independent effects. The reduction of the inflammatory infiltrate extension and the increase in the expression of M2 markers are IL-10-dependent effects of fenofibrate, while the reduction of proinflammatory mediators expression, cardiac fibrosis and the restoration of cardiac function are IL-10 independent effects.",yes
PMC3427844,Figure_2,oa_package/fe/6c/PMC3427844.tar.gz,"['The gross specimen was a yellowish solid mass that microscopically was compatible with papillary type RCC, Fuhrmann nuclear grade III ().']","FIG. 2 (A) Partial nephrectomy specimen showed a yellowish solid portion in the cystic mass (B) that was compatible with papillary type renal cell carcinoma, Fuhrmann nuclear grade III (H&E, 200).",yes
PMC10349966,Figure_6,oa_package/53/dd/PMC10349966.tar.gz,"['Using confocal microscopy, we observed considerably fewer cells expressing SORL1 at the cell surface in cells transfected with SORL1-R953C compared to SORL1-WT (a).', 'Results were consistent in both HEK293 and N2a cells (b c).', '547348v3-f0005"" position=""float""/>.']","Figure 6. SORL1 R953C cells have reduced SORL1 protein localization on the cell surface. (a) Representative immunocytochemistry from HEK293 cells transiently transfected with SORLA-WT or SORLA-R953C expression construct and stained for SORLA (red) at the cell surface. White arrows show positive cells. (b) Flow cytometry dot plot showing surface (AlexaFluor 647 fluorescence) and total (GFP fluorescence) in live single HEK293 cells expressing WT-GFP and R953C-GFP. Vertical and horizontal lines represent thresholds for GFP and AlexaFluor 647-positive cells, respectively. Represented are GFP-positive cells with AlexaFluor 647 signal above (black, inside red dashed gate) or below threshold (dark grey); and untransfected cells (light grey). Numbers in the plots represent the percentages of the cells inside the gates. (c) Bar plots of AlexaFluor 647 fluorescence in HEK293 and N2a cells expressing WT-GFP or R953C-GFP, generated from population of GFP-positive cells. n=3 independent experiments. Results are expressed as Mean SD and analyzed by parametric two-tailed paired t-test. Significance was defined as a value of ***p<0. 001.",yes
PMC2790372,Figure_5,oa_package/33/bf/PMC2790372.tar.gz,"['These parameters were not significantly different (A and 5B).', 'We did not detect obvious differences between A 42 and control fly brains (C).', 'We also compared that cAMP levels in head extracts of A 42 and control flies, and found they were not significantly different (D and The proportion of double readings related to the whole number of readings was homogeneous amongst the readers (median 4.']","Figure 3: CT annotations: Visual quantification of lung disease extent. The readers had to visually quantify the extent of COVID-19 pneumonia on a 5-point scale. Here, it is estimated to be more than 50% and less than 75% (50-75%). Readers were also asked to manually contour COVID-19 pneumonia (area in blue in the right lung) on at least 2 CT images to later train deep learning algorithms for automated quantification of disease extent.",yes
PMC6879599,Figure_3,oa_package/28/a8/PMC6879599.tar.gz,"['The final tractography images are shown in .', 'The tractography results closely resemble classical descriptions of OR anatomy derived from dissection studies, with a continuous sheet-like structure ().']","FIGURE 3 Final optic radiation tractography images generated from all raters. The tractography images are color coded by tract directions: left-right (red), superior-inferior (blue), and anterior-posterior (green). The MRI images are displayed in radiology convention. L, left; R, right. Rater A (B)- first/second = first/second tractography attempt by rater A (B). Hemisphere side colored by lesion/pathology: red, lesional; white, non-lesional.",yes
PMC9315489,Figure_3,oa_package/54/23/PMC9315489.tar.gz,"['Effects of Chemotherapeutic Agent, Doxorubicin, upon Cellular Volatile ProfilesDoxorubicin treatment produced significant alterations in the volatile profile of both MCF10A and MDA-MB-231 cells, as shown in .', 'Doxorubicin induces volatile response in breast cell lines.']",Figure 3 Doxorubicin induces volatile response in breast cell lines. ( ) Boxplot for select volatile organic compounds (median Tukey distribution; = 6). ANOVA followed by Tukey post hoc test was performed; * < 0.05; ** < 0.01; *** < 0.001; **** < 0.0001. Doxorubicin has been abbreviated to Dox.,yes
PMC7394155,Figure_3,oa_package/a7/50/PMC7394155.tar.gz,"['HE 100 , bar = 100 mMale rat from the control group.']","Figure 3 Male rat from the control group. Type I collagen 200, bar = 50 m",yes
PMC9106548,Figure_3,oa_package/98/d8/PMC9106548.tar.gz,"['Photomicrograph (x100, x400, hematoxylin and eosin stained)Image showing epidermis with irregular hyperplasia (yellow arrows) , parakeratosis (yellow square), superficial and deep dermal infiltrates perivascular and interstitial; epidermotropism (red square); nodular lymphoid aggregates located in the deep dermis (red arrow)Immunohistochemical stainingImage revealing positivity for CD2 and CD4 and negative results for CD3 and CD5 markersIn addition, blood tests revealed inflammatory syndrome (erythrocyte sedimentation rate (ESR) 32 mm/h), leukocytosis with neutrophilia, and elevated lactate dehydrogenase (LDH) (701 U/L).']","Figure 3 Photomicrograph (x100, x400, hematoxylin and eosin stained) Image showing epidermis with irregular hyperplasia (yellow arrows) , parakeratosis (yellow square), superficial and deep dermal infiltrates perivascular and interstitial; epidermotropism (red square); nodular lymphoid aggregates located in the deep dermis (red arrow)",yes
PMC2804724,Figure_2,oa_package/e6/ea/PMC2804724.tar.gz,"['(A) Histological appearance of the lesion in Case 1: the neoplasm is rich in cells of mesenchymal origin; stroma presents myxoid and hyalinization aspects and small foci of necrosis, while fibrous-histiocitic cells displays a storiform or cartwhell pattern (Haemotxylin and Eosin, original magnification 20).', '(B) Higher magnification of figure 2A, better showing the cartwhell pattern of the fibrous-histiocitic tumour cells (Haematoxylin and Eosin, original magnification 40).']","Figure 2 . Higher magnification of figure 2A, better showing the cartwhell pattern of the fibrous-histiocitic tumour cells (Haematoxylin and Eosin, original magnification 40).",yes
PMC3670857,Figure_3,oa_package/ef/69/PMC3670857.tar.gz,"['In addition, histology of the brain and spinal cord did not reveal any major differences when examined using LFB (myelin) or PAS (globoid cells) staining (C and G).', 'g003LFB/PAS staining of Twitcher mice lacking KC or CXCR2.', 'When the brains of CXCR2 / GALC / mice were compared to CXCR2+/+GALC / mice, there was no qualitative difference in LFB/PAS staining between the two groups (D and H).']",10.1371/journal.pone.0064647.g003,yes
PMC10178249,Figure_1,oa_package/d3/70/PMC10178249.tar.gz,"['Three months after treatment, grade 2 uptake was increased in all vascular territories ().', '1038/s41573-021-00198-133976384A patient with the diagnosis of melanoma with ongoing immune checkpoint inhibitors treatment without a history of cardiovascular diseases or clinical evidence of vasculitis.']","Figure 1 A patient with the diagnosis of melanoma with ongoing immune checkpoint inhibitors treatment without a history of cardiovascular diseases or clinical evidence of vasculitis. [ F]FDG PET/CT maximum intensity projections ( , ), as well as coronal ( , ) views, three and six months after treatment initiation, are shown. The mild increase in uptake intensity of the aorta and subclavian arterial walls was visually seen compared to the pretreatment ( , , arrows) images. Semi-quantitative analyses by SUVmax were correlated with visual findings showing a mild increasing pattern of [ F]FDG uptake suggestive of immunotherapy-related mild arterial wall inflammation.",yes
PMC8585837,Figure_10,oa_package/87/58/PMC8585837.tar.gz,[],"Fig. 10 Chronic retroplacental hemorrhage resulting in fetal death at 26weeks. The placental disc weighed 85g. Coiling index 1 coil/5cm. Acute retroplacental hemorrhage: Stillbirth at 37+5weeks. The placental disc weighed 425g with a loosely adherent clot measuring 100x100x10mm. Note no obvious compression of the disc. Retroplacental hemorrhage in a 30week gestation placenta associated with intrauterine growth restriction (900g). The mother had severe pre-eclampsia. The placental disc weighed 280g. Note the disc compression, intervillous hemorrhage and early villous infarction (H&E, Original magnification 12.5)",yes
PMC8670046,Figure_2,oa_package/71/46/PMC8670046.tar.gz,"[' 2).', '2).', 'Histological and Immunostaining analysis of 7 age groups studied in the protocol (Gr1; Gr2; Gr3; Gr4; Gr5; Gr6; Gr7) for 4 conditions.', '*: Leydig cells,: peritubular cells nuclear AR staining membranous Cx43 stainingHistological and Immunostaining analysis of control groups compared to Complete Androgen Insensitivity Syndrome (CAIS) and Leydig Cell Tumor (LCT) groups for 4 conditions.', '2-7B).', '2-7D).', '2-7C).', '2).']","Fig. 2 Histological and Immunostaining analysis of 7 age groups studied in the protocol (Gr1; Gr2; Gr3; Gr4; Gr5; Gr6; Gr7) for 4 conditions. HPS staining: Hematoxylin, Phloxine, Safran -Immunohistochemistry staining specific: Androgen Receptor (AR); Connexin 43 (CX43); Anti-Mullerian Hormone (AMH). Morphological aspects and immunohistochemistry profile with AR, Cx43 and AMH in the 7 age groups of normal testis. The seminiferous tubules have a prebubescent appearance (small tubules without lumen and spermatogonia) in group 1 to 5 (1A, 2A, 3A, 4A, 5A). In group 6, the tubules lengthen, central lumen become visible (6A). Leydig cells (*) are observed during minipuberty (2A, 3A) and more numerous at puberty (7A), immunostained by Cx43 (2C, 3C). No Leydig cell is observed in the other age groups (1A, 4A, 5A, 6A). Expression of AMH is specific by Sertoli cells decrease with age (1D, 2D, 3D, 4D, 5D 6D) and is almost zero at puberty (7D). Membranous staining of CX43 in Sertoli cells () is only present at puberty (7C). The AR expression in Sertoli cells () is very weak during minipuberty (2B), then turns negative (3B, 4B, 5B) to gradually reappear with significant expression in group 6 (6B) and strong expression in group 7 (7B). Peritubular cells () are marked by immunostaining for AR in all age group (1B, 1B, 3B, 4B, 5B, 6B, 7B). *: Leydig cells,: peritubular cells nuclear AR staining membranous Cx43 staining",yes
PMC2627247,Figure_7,oa_package/28/30/PMC2627247.tar.gz,"['Percutaneous, radiological catheter drainage is an effective and safe method for the treatment of abdominal abscesses associated with fistulas and can substitute for surgery ().', 'Percutaneous management of high-output fistulas.']","Fig. 7 Percutaneous management of high-output fistulas. After Billroth I operation, abdominal CT image shows abnormal fluid collection at anastomotic site and peri-pancreatic portion. After drainage of large abscesses, significant decrease in fluid collection is seen. Communication to duodenal bulb is noted. Two month follow-up upper GI series shows disappearance of fistulous tract and abscess.",yes
PMC6466941,Figure_1,oa_package/47/de/PMC6466941.tar.gz,"['6%), including renal artery stenosis (), carotid artery stenosis, descendant aortic disease, and iliac and femoral artery disease.', '0-8490250017924939071Bilateral renal artery stenosis.']",Figure 1 Bilateral renal artery stenosis.,yes
PMC10467011,Figure_2,oa_package/f0/7f/PMC10467011.tar.gz,"['Too gentle or too strong pretreatments may affect the subsequent detection negatively ().', '.', '1177_01926233231178282-fig2"" position=""float""/>HybridizationThe next step after tissue/cell permeabilization is the probe hybridization with the target sequence.', '1177/01926233231178282"" ext-link-type=""uri"">Supplementary ).', '1177/01926233231178282"" ext-link-type=""uri"">Supplementary ).']","Figure 2. Optimization of pretreatment conditions for in situ hybridization. Example of differently pretreated liver sections from a cynomolgus macaque, where different pretreatment in 1X retrieval buffer at 98C to 104C for 20 (A), 30 (B), and 40 minutes (C). Note that 30-minute pretreatment gave the best signal for a housekeeping gene used as positive control-cynomolgus PPIB (peptidyl-prolyl cis-trans isomerase B), whereas under (20 minutes) or over treatment (40 minutes) was not optimal.",yes
PMC9437290,Figure_1,oa_package/10/02/PMC9437290.tar.gz,[],"Figure1 Pathological findings of renal biopsy. Light microscopy shows a MPGN pattern of glomerular lesion , HE; , PAS; , PASM; , MASSON, 400). Immunofluorescence depicts granular mesangial and wall deposits with IgG, C1q, kappa, lambda, IgG3, and IgG2 trace, while IgG1 and IgG4 are negative [ , 400].",yes
PMC6918090,Figure_1,oa_package/18/90/PMC6918090.tar.gz,"['monocytogenes CFU shed in the mouse feces over the course of 5 days (', 'As a gross metric of disease severity, we monitored body weight over the course of infection (', 'FIG 1Streptomycin pretreatment enhances susceptibility of mice to foodborne L.', 'monocytogenes CFU were enumerated in the gastrointestinal tract, mesenteric lymph nodes, spleen, liver, and gallbladder (', 'monocytogenes was not detectable, all the gallbladders of Sm mice contained 106 CFU (', 'However, inocula of only 100 bacteria led to weight loss during the course of infection (', 'monocytogenes robustly replicates within the streptomycin-pretreated intestines (', 'monocytogenes population structures began to deviate from the input population, which coincided with the onset of diarrhea and weight loss in the animal (']","FIG1 Streptomycin pretreatment enhances susceptibility of mice to foodborne (Lm) infection. (A) fecal shedding. C57BL/6 mice ( =5) received either no treatment or streptomycin (Sm)-supplemented drinking water for 48h prior to infection by voluntary consumption of bread containing 10 CFU. Results are expressed as log-transformed CFU per gram of feces. Lines represent individual mice. Treatment: < 0.001 (two-way analysis of variance [ANOVA]). (B) Body weight of mice in panel A. Results are expressed as a percentage of body weight prior to streptomycin treatment. Lines represent individual mice. Treatment: < 0.001 (two-way ANOVA). (C) Bacterial burden 5days postinfection of mice in panel A. Results are expressed as log-transformed means with standard errors. ***, < 0.001 by unpaired two-sided test. Dashed lines indicate limits of detection. (D) Body weights of mice receiving Sm and either 10, 100, or 1,000 CFU as described for panel A. Results are expressed as a percentage of body weight prior to streptomycin treatment, and means with standard errors are indicated. Two-way ANOVA, dose, < 0.05; Bonferronis posttest, 10 versus 100, day 5: < 0.001; 10 versus 1,000, day 5: < 0.01). (E) fecal shedding in mice in panel D. Results are expressed as log-transformed means with standard errors. Two-way ANOVA, dose, < 0.001; Bonferronis posttest, 10 versus 100, day 2: < 0.001; 10 versus 1,000, day 2: < 0.001). Dashed line indicates limit of detection. All results are representative of at least 2 independent experiments.",yes
PMC3015936,Figure_4,oa_package/1c/5b/PMC3015936.tar.gz,['.'],Figure 4. Caseating granuloma caused by hemostatic agent low power image of caseating granuloma.,yes
PMC9792064,Figure_2,oa_package/71/a0/PMC9792064.tar.gz,"['CXCL10 immunostaining positive slide showing scattered positive lymphocytes (before treatment with NB-UVB)CXCL10 negative lymphocytes (after treatment with NB-UVB)\nMeasurement of serum CXCL10\nA blood sample was obtained for measurement of CXCL10 level by enzyme-linked immunosorbent assay technique (ELISA) via human CXC-chemokine ligand 10 ELISA kit (bt-laboratory, China, No E3800Hu).', 'Immunostaining showed positively stained lymphocytes before treatment with NB-UVB [] and negatively stained lymphocytes after treatment with NB-UVB [].']",Figure 2 CXCL10 immunostaining positive slide showing scattered positive lymphocytes (before treatment with NB-UVB),yes
PMC11122514,Figure_4,oa_package/27/6a/PMC11122514.tar.gz,"['In addition, hyperplasia and basal hyperpigmentation were found in the epidermis, and the underlying dermis had a dense lympho-histiocytic infiltrate ().', 'Histological examination, hematoxylin eosin stain (10 ): melanocyte proliferation at the dermo-epidermal junction, atypical melanocytes arranged lentiginously and continuously; pagetoid invasion of the epidermis and formation of anisomorphic nests; lympho-histiocytic dermal infiltrate.']","Figure 4 Histological examination, hematoxylineosin stain (10): melanocyte proliferation at the dermo-epidermal junction, atypical melanocytes arranged lentiginously and continuously; pagetoid invasion of the epidermis and formation of anisomorphic nests; lympho-histiocytic dermal infiltrate.",yes
PMC11426651,Figure_4,oa_package/9f/97/PMC11426651.tar.gz,"['A month later, a CT showed lipomatosis of the terminal ileum and ileocecal valve with diffuse intramural fatty infiltration .', 'jpbs_449_24-f004"">Abdominal computer tomography showed lipomatosis of the terminal ileum and ileocecal valve with diffuse intramural fatty infiltration.']",Figure 4 Abdominal computer tomography showed lipomatosis of the terminal ileum and ileocecal valve with diffuse intramural fatty infiltration. Coronal (a) and axial (b) plane (red arrow),yes
PMC10710783,Figure_9,oa_package/59/5f/PMC10710783.tar.gz,"['Finally, white spots were observed in some non-drug sites of the mice (A).', 'The results of HE staining under light microscope showed that the epidermis of the monobenzone-induced vitiligo model group of mice showed local epidermis thickening, more inflammatory cell infiltration in the superficial dermis, some hair follicles were abnormal and dysplasia, and the number of melanin-containing hair follicles was significantly reduced (B).', '(A) Vitiligo mouse models: the red arrows indicate the MBEH application site, where the hair is significantly whitened.']","Figure 9 ( ) Vitiligo mouse models: the red arrows indicate the MBEH application site, where the hair is significantly whitened. ( ) HE staining results: the number of hair follicles in the control group was significantly higher than that in the vitiligo group; the distribution of melanin around the hair follicle was obvious.",yes
PMC8429860,Figure_1,oa_package/29/0a/PMC8429860.tar.gz,"['Possible associated extra-cardiac malformations may include many organ systems: renal (agenesis, dysplasia) and gastrointestinal disease (), abdominal wall defects (), spine defects, and more (24).', 'Typical ultrasound finding in duodenal atresia ( double bubble ), with a dilated stomach and proximal duodenum.']","Figure 1 Typical ultrasound finding in duodenal atresia (double bubble), with a dilated stomach and proximal duodenum. High association with trisomy 21.",yes
PMC10507234,Figure_5,oa_package/6f/d4/PMC10507234.tar.gz,"['To this end, protein expression of -synuclein and -synuclein phosphorylated in serine 129 (p- -synuclein) in the midbrain was first measured ( 5A), the protein level of -synuclein and p- -synuclein in CS2-exposed rats showed a significant increase compared with the control ( 5B).', 'Next, we further examined p- -synuclein expression in frozen sections ( 5C).', 'Quantification of p- -synuclein and TH co-staining, indicative of the -synuclein phosphorylation within a dopaminergic neuron, showed a significant increase in CS2-exposed rats compared with the control (s 5D and 5E).', 'Because CS2 can react directly crosslink with some proteins in mammals to form adducts,33 we conducted an in vitro reaction system to further determine whether CS2 can directly induce -synuclein aggregation and phosphorylation ( 5F); the protein expression level of -synuclein and phosphor- -synuclein was measured and significantly increased in CS2-exposed groups than in the control at 37 C, overnight, and non-reduced environmental conditions ( 5G).', ' 5CS2 induces -synuclein aggregation and phosphorylation in dopaminergic neurons(A and B) The midbrain protein of the rat was extracted and immunoblotted with anti- -synuclein (anti- -Syn) and anti-phosphorylated -synuclein (anti-p- -Syn) antibodies (A), and the protein level was quantified (B).']","Figure4 CS induced necroptotic signaling activating and cell loss was attenuated by GSK872 (A) SH-SY5Y cells was cultured and exposed to dose-sequence CS or exposed to 10mM CS which pre-intervention with 5M GSK872. (B and C) Protein of SH-SY5Y cells that dose-sequence CS exposed was extracted and immunoblotted with anti-MLKL, and anti- -MLKL antibody (B), and the proteins level was quantified (C). (D and E) Protein of SH-SY5Y cells that GSK872 interferes was extracted and immunoblotted with anti-MLKL and anti- -MLKL antibody (D), and the proteins level was quantified (E). (F and G) SH-SY5Y cells after GSK872 intervention were pictured using a light microscope (F)and counted (G). (C), (E), and (G)show means SEM, p value is comparison with control group by t test",yes
PMC10509213,Figure_3,oa_package/b6/3b/PMC10509213.tar.gz,"[' 3), which can detect translocations, deletions, and duplications of the MLL gene.', 'Representative images of the cell nuclei, which were obtained by the FISH analysis using the MLL breakapart probe.', 'MLL rearrangements in PBL of radiologists (R) and controls (C).']","Figure 3 Representative images of the cell nuclei, which were obtained by the FISH analysis using the MLL breakapart probe. ( ) Cell contains two intact MLL genes, each visualized with co-localization of green and red signals, which mark two parts of the MLL gene. While the upstream (red) signal represents the MLL segment between the breaking point and gene PHLDB1 (Pleckstrin homology like domain family B member 1) closer to the telomere, the MLL segment between UBE4A gene (Ubiquitination factor E4A) and breaking point closer to the centromere is shown by green staining (downstream signal). ( ) Cell with translocation of the MLL gene, which is visualized as one red (upstream) and one green (downstream) signal. ( ) Cell with duplication of the red (upstream) signal, which is manifested by the presence of one redundant red signal. The MLL deletion is visualized by loss of the green (downstream) signal ( ), red (upstream) signal ( ) or whole MLL gene ( ).",yes
PMC9316444,Figure_3,oa_package/9b/f2/PMC9316444.tar.gz,"['This strengthens the observation that dinospores may have a higher specificity for gill epidermal cells, possibly through the anchorage and fixation processes (see ) to these cells [23].', 'Wet mount of gilthead seabream (Sparus aurata) gills at 5 h post-infestation with Amyloodinium ocellatum.']","Figure 3 Wet mount of gilthead seabream ( ) gills at 5 h post-infestation with ( ) Dinospore (DIN) with a filamentous structure (black arrow) attached to the gill (400); ( ) Dinospores presenting with an elongation, characteristic of the transition phase toward the trophont stage (black arrowhead), and the connecting filament (black arrow) (200); ( ) Detail of the image in panel B, showing the filamentous structure (black arrow) that connects the dinospore to the gill (400).",yes
PMC2801609,Figure_1,oa_package/db/bc/PMC2801609.tar.gz,"['0008626-Crosby1"" ref-type=""bibr"">[20] and our own analyses, dFKBP59c01413, dFKBP59k00424 and dFKBP59k09010 are viable loss-of-function mutants and have no effect on the morphology of the eye (A and 0.05.",yes
PMC7447682,Figure_5,oa_package/b9/2d/PMC7447682.tar.gz,"[' 5b).', '5c).', '5d, e).', '5f, g) and spleen (', '5h, i).', '5j, k) and spleen (', '5l, m) at day 10 p.', 'Increased numbers of CD4 T cells, CD4 T effector memory/effector cells, germinal centres and T follicular helper cells in the MLN and spleen of IgMi mice by day 10 p.', '001, Student s t testRNA seq analysis on B cells from MLN of IgMi and WT littermates at na ve, d10 and d21 p.']","Fig. 5 Increased numbers of CD4 T cells, CD4 T effectormemory/effector cells, germinal centres and T follicular helper cells in the MLN and spleen of IgMi mice by day 10 p.i.. IgMi mice and WT littermates were infected with ~200 embryonated eggs and necropsied at day 10 p.i.. Nave T cells were defined as CD3+CD62L+CD44 and T effectormemory/T effector cells were defined as CD3+CD62LCD44+. GC and T cells were defined as CD19+GL7+CD38 and CD4+CXCR5+PD-1 , respectively. Gating strategy ( ). Relative percentage and total cell number of CD4 TEM_TE in MLN ( , ) and spleen ( , ). Relative percentage and total cell number of T in MLN ( , ) and spleen ( , ). Relative percentage and total cell number of GC in MLN ( , ) and spleen ( , ).. Data show mean and SEM, pooling from 2 independent experiments, =5, males. * <0.05, ** <0.01, ***p <0.001,Students test",yes
PMC11407995,Figure_2,oa_package/61/79/PMC11407995.tar.gz,"['Histopathological examination revealed a hypercellular mesenchymal tumor comprising small to medium-sized spindle cells with small nucleus, indistinct nucleoli, and scant eosinophilic cytoplasm admixed with adipose tissue ().', 'Histopathological examination wide excision of the lesion.', 'No sign of necrosis and atypical mitoses was seen.']","Fig. 2 Histopathological examination wide excision of the lesion. Hypercellular mesenchymal tumor extending throughout the biopsy with no mitotic activity and necrosis admixed with adipose tissue (A, hematoxylin and eosin staining, original magnification 10; B, hematoxylin and eosin staining, original magnification 20).",yes
PMC11509416,Figure_3,oa_package/5a/b9/PMC11509416.tar.gz,"['Alkaline Phosphatase Activity and Calcium DepositionAlkaline phosphatase (ALP) activity, an early marker of osteogenesis, was measured in spheroids treated with PDRN at concentrations of 0, 25, 50, 75, and 100 g/mL on Days 7 and 14 (A).', 'Calcium deposition, a marker of late-stage osteogenesis, was assessed on Days 7 and 14 (B,C).', 'Osteogenic differentiation in PDRN-treated spheroids.']",Figure 3 Osteogenic differentiation in PDRN-treated spheroids. ( ) Alkaline phosphatase activity in PDRN-treated spheroids measured on Days 7 and 14. ( ) Evaluation of calcium deposition in PDRN-treated spheroids on Days 7 and 14. ( ) Quantitative analysis of calcium deposition in spheroids treated with different PDRN concentrations. * < 0.05 on day 14 compared to the time-matched unloaded group.,yes
PMC6829593,Figure_1,oa_package/85/28/PMC6829593.tar.gz,"['Consistent with our previous findings, TE4 mice treated with control chow showed severe brain atrophy predominantly in the hippocampus, piriform/entorhinal cortex, and amygdala, accompanied by significant dilatation of the lateral ventricle ().', 'In contrast, TEKO mice were largely protected from tissue loss and showed preserved brain volume ().', 'Strikingly, when microglia were depleted in TE4 mice for merely 3 mo, specifically during the stage when neurodegeneration begins and rapidly deteriorates, neurodegeneration was virtually fully blocked, and the brain volume of these mice remained the same as that of non-tau transgenic mice ().', '.', 'ApoE regulates neurodegeneration in P301S mice predominantly by regulating microglia-mediated innate immune responseAs we had described previously (Shi et al.', ', 2017), TEKO mice consistently showed a marked reduction of brain atrophy compared with TE4 mice ().', 'While there was a slight trend, PLX3397-treated TEKO mice did not show a significantly increased brain volume compared with control TEKO mice ().', 'Intriguingly, by depleting microglia, the effect of apoE on neurodegeneration was abolished, with PLX3397-treated TE4 and TEKO mice showing equal brain volumes ().']","Figure 1. Representative images of mouse brain sections stained with Sudan black for 9.5-mo-old TE4, TEKO, E4, and EKO mice treated with control or PLX3397-supplemented chow. Scale bar = 1 mm for all images. Quantification of brain volumes in the hippocampus and the piriform/entorhinal cortex (Ent/Piri Ctx) of the mice (TE4-Ctl: = 21; TE4-PLX: = 17; TEKO-Ctl: = 19; TEKO-PLX: = 18; E4-Ctl: = 12; E4-PLX: = 6; EKO-Ctl: = 13; EKO-PLX: = 6). Data are expressed as means SEM. One-way ANOVA with Tukeys post hoc test (two-sided) was used for statistical analyses. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. The Sudan black staining and brain volume quantification was performed once.",yes
PMC3038979,Figure_1,oa_package/80/dd/PMC3038979.tar.gz,"['Immunohistochemical staining of SOX2 shows different expression levels in early-stage breast carcinoma samples.', 'com/1471-2407/11/42/prepubSupplementary MaterialAdditional file 1Supplementary : Gene expression of SOX2OT and ALX4 in fresh frozen tumor samples.']",Figure 1 (A) Classification of SOX2 expression in different scores. (B) Staining of normal breast tissue as control. (C) Breast tumor tissue that shows no positive staining for SOX2 are part of Score 0. (D) Tumor samples with > 0% and < 10% are referred to Score 1. (E) Score 2 samples show 10% and < 50% positive stained cells. (F) Samples demonstrating 50% positive cells belong to Score 3. Pictures were taken with 200X magnification,yes
PMC9700639,Figure_3,oa_package/cd/5d/PMC9700639.tar.gz,"[' 3, no significant difference in body weight was detected in all experimental groups for the first 13 days of Cup administration.', 'The effect of Neb on Cup-induced body weight changes.', 'Statistical analysis was performed using two-way ANOVA followed by Bonferroni test for multiple comparisons between groupsNeb attenuated cup-induced demyelinationMyelin status was assessed by LFB staining of the CC and western blotting of MBP.']","Fig. 3 The effect of Neb on Cup-induced body weight changes. , :Statistically significant from the control and Cup-treated groups, respectively, at <0.05. Statistical analysis was performed using two-way ANOVA followed by Bonferroni test for multiple comparisons between groups",yes
PMC4719406,Figure_3,oa_package/1d/43/PMC4719406.tar.gz,['Histology of the lesion at 10x.'],Figure 3 Histology of the lesion at 10x. Histology of the lesion showing papillary projections lined by hyperplasic synovial cells infiltrated by lymphocytes and few plasma cells with hemosiderin consistent with a histology of pigmented villonodular synovitis. Histology specimen at 100x in the left upper corner.,yes
PMC8996004,Figure_3,oa_package/0e/66/PMC8996004.tar.gz,"['We report the TCE, FPR and FNR in figure 3, and IGSD of those three metrics in figure 4.', 'We present results for EO3 in figure 3, as it was the only equalised odds model that achieved a reduction of IGSD (FPR) while keeping a low IGSD (FNR) at both thresholds.', 'Model performance across evaluation metrics, stratified by demographic group, evaluated on the test set.', '831)), while maintaining differences of AUROC between groups (figure 3A).', '019)) (figure 3A).', 'Additionally, at each threshold, for all models, we observe a relationship between TCE, FPR and FNR: increased TCE (overestimation) leads to higher FPR and lower FNR, and decreased TCE (underestimation) to lower FPR an higher FNR (figure 3B).', '8)) (figure 3A).', 'However, it did so by trading off error rates in opposite directions at the two thresholds, as described above (figure 3B).']","Figure 3 Model performance across evaluation metrics, stratified by demographic group, evaluated on the test set. The left panel showsAUROC and absolute calibration error. The right panel shows false negative rates, false positive rates and threshold calibration error at two therapeutic thresholds (7.5% and 20%). EO, equalised odds; PCEs, original pooled cohort equations; rPCE, revised PCEs; rUC, recalibrated model; UC, unconstrained model.",yes
PMC6981119,Figure_3,oa_package/12/b2/PMC6981119.tar.gz,"[' 3.', 'Relationship between LVL and IVPD, IVPGLVL showed a significant positive correlation with total IVPD (R = 0.']","Figure 3 The analysis results of 2 healthy dogs. The left sided figures ( , , ) are VFM derived data of medium-sized dog weighing 14kg and 45mm length of LV. The right sided figures ( ) are data of a small dog weighing 3kg and 22mm of LV length. ( ) visualize the stream line at early diastolic phase with the highest vorticity in the left ventricle. A vortex on the tip of the anterior mitral leaflet is visible in the left ventricle of both cases. ( , ) Show the contours of vorticity in the same time phase as the upper figures. Vorticity was highest at the location of the vortex seen in the figure above. The smaller heart on the right side has denser contours, representing higher vorticity. ( , ) Are the transition of the vorticity in a cardiac cycle. Diastolic vorticity is higher in the small heart on the right side. LV, left ventricle; LA, left atrium.",yes
PMC2980919,Figure_1,oa_package/ca/ea/PMC2980919.tar.gz,"['Snapshots of the pictures taken by the capsule looking at the lesion, first lesionSnapshot of the second lesionLesion seen intraoperatively; first lesion is big and obvious and the second lesion is relatively less prominent on the serosal sideResected specimen showing the lesion from the luminal sideDISCUSSIONCapsule endoscopy is a technology approved by the Food and Drug Administration, USA, for its use in pediatric age group between 10 and 18 years,[2] and involves swallowing a video capsule of size 26 11 mm [] to visualize and record its findings in the esophagus, stomach, and small intestine.']","Figure 1 Snapshots of the pictures taken by the capsule looking at the lesion, first lesion",yes
PMC9308607,Figure_5,oa_package/af/dd/PMC9308607.tar.gz,"[' 5).', '34-year-old male with Mustard repair of c-TGA and loop recorder implantation.', 'CCT cardiovascular computed tomography, CMR cardiovascular magnetic resonance, cTGA complete transposition of the great arteries, SSFP Steady State Free Precession, La left atrium, Lv left ventricle, Ra right atrium, Rv right ventricleCCT accurately quantifies right and left ventricular volumes and function [44, 45], although with lower temporal resolution than CMR or echocardiography and at the expense of increased radiation exposure, needing ECG-synchronized data acquisition during the whole cardiac cycle (i.']","Fig. 5 34-year-old male with Mustard repair of c-TGA and loop recorder implantation. CMR SSFP 4-chamber view shows atrio-ventricular concordance and the pulmonary baffle (asterisk). The ventricular apex is canceled by artifacts ( ). 18-year-old Fontan patient with pacemaker implantation. CCT axial plane displays the wires and the pacemaker generator (arrows) with minimal artifacts upon thoracic aorta ( ). Adult male patient with Mustard repair of c-TGA and baffle leakage. CMR SSFP 4-chamber images show the flow turbulence (arrow) before the treatment ( ) and a huge artifact (arrow) caused by the metallic closure device ( ). Adult male with ASD after endovascular closure. CCT axial image well depicts the closure device without limitations to cardiac chambers visualization ( ). cardiovascular computed tomography, cardiovascular magnetic resonance, complete transposition of the great arteries, Steady State Free Precession, left atrium, left ventricle, right atrium, right ventricle",yes
PMC3518957,Figure_3,oa_package/8b/24/PMC3518957.tar.gz,"['After access cavity preparation, pulp was removed and three root canals were exposed ().', '002""/>Root canal orifices after enlargement during endodontic treatment.']",Figure 3 Root canal orifices after enlargement during endodontic treatment.,yes
PMC8593330,Figure_3,oa_package/a9/1b/PMC8593330.tar.gz,['A 15-day-old female newborn with CPSF on the right neck.'],"Figure 3 A 15-day-old female newborn with CPSF on the right neck. US and CT showed a non-septal cystic mass in the right neck. US showed an inhomogeneous mass with low echogenicity, in which there were no air bubbles . Color Doppler showed there was no blood flow sonogram in the cyst . CT showed that the cyst wall was thick and high attenuation, the border of the cyst was poorly defined, and tiny air bubbles could be seen in the cyst (arrowhead). The cystic mass extended laterally into the retropharyngeal space and longitudinally into the anterior mediastinum .",yes
PMC10604867,Figure_17,oa_package/8b/81/PMC10604867.tar.gz,[],Figure 17 Clinical picture of multiple lentigines and cafe noir spots in Noonan syndrome with multiple lentigines ( ). Dermoscopy reveals brown pigmentation in a cobblestone pattern ( ).,yes
PMC5686970,Figure_4,oa_package/dd/9f/PMC5686970.tar.gz,"['Majority were astrocytoma which mostly presented between 15 and 65 years, while most of the pilocytic astrocytomas were in children [].', 'Whereas, the most difficulty was found in the case of oligodendroglioma [], due to the resemblance of this glioma with other low-grade glioma, lack of calcification, and no cytoplasmic clearing [Table 2 and ].', '(d) Medulloblastomas having carrot cells with spongioblastic arrangement (H and E, 40)Oligodendroglioma (a) Magnetic resonance imaging Solid cystic sol in right frontoparietal region.']","Figure 4 Oligodendroglioma (a) Magnetic resonance imaging Solid cystic sol in right frontoparietal region. (b) Cells form vague clusters. The smear disperses many of the nuclei away from the clusters. A weak or sparse glial matrix loosely ties the cells into these collections (H and E, 100). (c) Calcification (H and E, 100). (d) Halos in oligodendrogliomas (H and E, 400)",yes
PMC8282135,Figure_4,oa_package/d3/6d/PMC8282135.tar.gz,"[', tenderness at the acromial area without radiographically confirmed fracture but with ultrasonographically confirmed cortical discontinuity28,32 on the tender point suspected of having fracture(); and (3) acromial stress fracture (ASF), i.', '\n11\n.', '1177_24715492211022171-fig4"" position=""float""/>Statistical AnalysesThe SPSS software package (version 21.']","Figure 4. Acromial stress occult fracture site. A, Longitudinal ultrasonography. B, Transverse ultrasonography. The fracture was diagnosed based on a radiographic finding of cortical discontinuity and periosteal thickening.",yes
PMC10206902,Figure_4,oa_package/fe/c8/PMC10206902.tar.gz,"['Furthermore, some bronchi lost air content because of fluid collection ().', '18In the present study, both temporal CT images () and pathological findings confirmed an increase in lung field density indicating pulmonary edema and increasing discharge to the peripheral bronchi.', '19 21 We speculate that cessation of these cell functions after 12 hours postmortem may have led to the results shown in .', 'Fluid in the trachea and bronchiThe bronchus on the ventral side of the right lung (arrow) shows loose air due to fluid accumulation 48 hours postmortem.']",Figure 4 Fluid in the trachea and bronchi The bronchus on the ventral side of the right lung (arrow) shows loose air due to fluid accumulation 48 hours postmortem.,yes
PMC8999690,Figure_2,oa_package/22/11/PMC8999690.tar.gz,"['Modified Sgarlato s [18,30,31,32], , is the angle between the longitudinal axis of the second metatarsal and the longitudinal axis of the lesser tarsus using the 5th metatarso-cuboid joint as a reference.', 'Dorsoplantar weightbearing radiograph of modified Sgarlato s angle.']",Figure 2 Dorsoplantar weightbearing radiograph of modified Sgarlatos angle.,yes
PMC10590186,Figure_3,oa_package/de/45/PMC10590186.tar.gz,"['80%) (, Table 1).', 'tif""> A) Hematoxylin and eosin (H E) stained sections show a mixed infection of septate acute angle branching hyphae of Aspergillus along with broad aseptate\nhyphae of Rhizopus.']",Figure 3 A) Hematoxylin and eosin (H&E) stained sections show a mixed infection of septate acute angle branching hyphae of Aspergillus along with broad aseptatehyphae of Rhizopus. B) The H&E-stained sections show the invasion of the vascular wall and lumen filled with broad aseptate fungal hyphae of mucormycosis (100x).,yes
PMC11488108,Figure_3,oa_package/26/4e/PMC11488108.tar.gz,['(A) The apoptosis rate of OA-damaged PC12 cells after incubation with NPs were measured by flow cytometry through PI/Annexin-V kit.'],"Figure 2 (A) Confocal microscopic images of PC12 cells after incubation with IR780-Mn@TA and IR780-Mn@TA-TPL NPs. DAPI: ex = 405, em = 420-450 nm, red fluorescence of NPs: ex = 775 nm, em = 790-820 nm. Scale bar: 20 m. (B) The relative cell viability of PC12 cells after treated with IR780-Mn@TA-TPL NPs of different concentrations. (C) The relative cell viability of PC12 cells after incubation with OA, following by different treatments with NPs. (D) Quantitative analysis of intracellular ROS level indicated by the fluorescence intensity of DCFH-DA probe via flow cytometry assay. (E) Representative microscopic images of MitoSOX staining in PC12 cell after different treatments. Red MitoSOX: ex = 561 nm, em = 575-625 nm. Scale bar: 20 m. (F) Quantitative analysis of intracellular MitoSOX fluorescence intensity via ImageJ software. Data were presented as mean SD. Significance: ** p < 0.01 and *** p < 0.001. (G) Representative fluorescence images of OA-damaged PC12 cells after various treatment stained with JC-1 probe to evaluate the mitochondrial membrane potential. Green JC-1 monomers: ex = 488 nm, em = 500-530 nm, red JC-1 aggregates: ex = 561 nm, em = 575-625nm. Scale bar: 10 m.",yes
PMC3702623,Figure_1,oa_package/b2/85/PMC3702623.tar.gz,['ChaouiRHoffmannJHelingKSThree-dimensional (3D) and 4D color Doppler fetal echocardiography using spatio-temporal image correlation (STIC)Ultrasound Obstet Gynecol23535545200415170792STIC image of the truncus arteriosus communis (Type I) of a 23-week fetus diagnosed by echocardiography.'],"Figure 1 STIC image of the truncus arteriosus communis (Type I) of a 23-week fetus diagnosed by echocardiography. Reconstructed images of (A) the STIC reverse imaging mode and (B) the STIC TUI imaging mode. STIC, spatio-temporal image correlation; TUI, tomographic ultrasound imaging; AO, aorta; PA, pulmonary artery.",yes
PMC7840595,Figure_4,oa_package/95/d1/PMC7840595.tar.gz,"['All statistical outputs for 2-way ANOVA (.', 'A, E, I, M, Q) and 1-way ANOVA (.', 'For each animal, 1 rostral and 1 caudal section corresponding to the shaded rostral and caudal border regions in .', 'Panning the complete 3D volume then helped verify anatomical features and explore how contusion pathology spreads through the spinal cord (quantified in ).', 'These tomograms are quantified in .', 'Serial transverse image slices at 0.', 'Unambiguous macro spinal cord features including total spinal cord area (.', 'A), tissue damage (debris + necrosis, .', 'E), gray matter (.', 'I), white matter (.', 'M) and dorsal column (.', '8 m length) at the epicentre (.', 'C, G, K, O, S) and at an equal distance rostral (.', 'B, F, J, N, R) and caudal (.', ""0001; Two Way RM-ANOVA with Tukey's post-hoc; ."", 'At 24 HPI, acute swelling mainly occurred within the injury epicentre (.', 'C) and caudal (.', 'Significant atrophy first appeared rostral to injury at 1 WPI (.', 'B), but by 5 WPI, atrophy reached significant levels across rostral, epicentre (.', 'C) and caudal (.', ""0001; Two Way RM-ANOVA with Tukey's post-hoc; ."", 'This pattern is similar across rostral (.', 'F), epicentre (.', 'G) and caudal (.', ""0001; Two Way RM-ANOVA with Tukey's post-hoc; ."", '3% loss by 5 WPI (.', '7% loss by 5 WPI (.', '0% at 24 and 72 HPI, respectively (.', ""0001; Two Way RM-ANOVA with Tukey's post-hoc; ."", '2% loss by 5 WPI (.', 'Rostral (.', 'N) and caudal (.', ""0001; Two Way RM-ANOVA with Tukey's post-hoc; ."", 'Q), with extensive loss of dorsal column tissue at the epicentre as soon as 24 HPI, plateauing to a loss of ~80% by 1 WPI (.', 'No significant change occurred in the rostral dorsal column volume relative to SHAM (.', '1% at 24 and 72 HPI, respectively (.', 'The dorsal column from .', '(D) By 5 WPI, caudal damage appears relatively limited to focal dorsal column lesions (yellow arrows), with gross damage replaced by an overall tissue atrophy (see quantification in ).', '1 rostral and 1 caudal section were used for quantification, which were matched to those rostral caudal regions as defined in the gray shading of .', 'mp4"">Supplementary Video 6Supplementary material Statistics associated with spinal cord injury feature quantification in .', 'All summary statistics associated with multiple comparisons made in .']","Fig. 3 Matched mid-sagittal and mid-longitudinal planes showing the emergence of acute-to-chronic tissue damage. The same spinal cords presented as transverse sections in are here projected along sagittal (i) and longitudinal (ii) planes. (A) At 24 HPI, damage at the injury site spans the width of the spinal cord, spreading centrally in rostro-caudal directions. The sagittal plane demonstrates local swelling of tissue in contact with the overlying dura matter (red arrows). The longitudinal plane also highlights satellite tissue damage regions (cyan arrows) separate from the epicentre. (B) At 72 HPI, swelling has subsided, but the injury extent has spread. (C) At 1 WPI the injury does not appear to have spread further, but debris appears more fragmented than at earlier time points. (D) By 5 WPI, tissue atrophy extending rostro-caudally to the injury epicentre is apparent, with obvious contraction of the injury epicentre. Yellow dashed lines in sagittal sections and pink dash lines in longitudinal sections indicate the relationship between sagittal and longitudinal planes.",yes
PMC10482757,Figure_1,oa_package/68/ab/PMC10482757.tar.gz,"['1A).', '1A).', 'Initial characterization of the cyli mouse model.', 'The cyli mutation leads to the formation of a PTC that is predicted to cause premature protein truncationGene identification and mutation analysisThe disease-causing locus was positioned to Chromosome 1 between markers D1Mit168 and D1Mit231 (D1Mit168 - 0.', '1B).', '1C) leading to a frameshift and predicted PTC within exon 48, 44 bp downstream of the T insertion (', '1D).']","Fig. 1 Initial characterization of the mouse model. Kidney (top) and liver (middle and bottom) tissue sections stained with hematoxylin and eosin (H&E) from 8-week-old WT and mice. Bottom panels are higher magnification views of the boxed areas in middle panels (BD, bile duct; C, cortex; M, medulla; PV, portal vein). Schematic illustrating the position of the mutation (affecting ) on Chromosome 1 between the genetic markers and (genetic distances are in centiMorgan (cM) units). Sequence comparison of from WT and mice (NM_153179.3: c.7588_7589delGGinsT (p.G2530VfsTer15). Comparison of WT and reading frames. The mutation leads to the formation of a PTC that is predicted to cause premature protein truncation",yes
PMC2699342,Figure_6,oa_package/a6/d0/PMC2699342.tar.gz,"['Relative mRNA expression of VEGF-A, Flt-1 and Flk-1 in the retina at 8 days after vessel ligation.']","Figure 6 . The relative expression of VEGF (A) and its Flt-1 receptor (F) is increased in the 2VO groups with respect to the sham group of animals while the relative expression of Flk-1 decreases in 2VO groups compared with sham-group (G). NGF injection reduced the damage-induced VEGF mRNA expression. Results were considered statistically significant when one-way ANOVA and post hoc Tukey's test, p < 0.01, p < 0.001; Student's test: *p < 0.05.",yes
PMC1872031,Figure_8,oa_package/14/be/PMC1872031.tar.gz,['1.'],Figure 8 1. Photograph of a patient showing swelling over left shoulder: later diagnosed as Hemangiopericytoma. 2. X-ray showing lesion involving the left clavicle. 3. Smear showing malignant round cells radiating from vessels. [MGG 400]. 4. Histological section of the same case showing monomorphic round cells radiating from cells [H&E 200].,yes
PMC7390021,Figure_1,oa_package/a1/ac/PMC7390021.tar.gz,"['The Enhanced Electrophysiology Visualization and Interaction System ( LVIS) system was developed to provide electrophysiologists with virtual 3D data placed within the clinical work area in stereoscopic 3D display ().', 'FIGURE 1.']","FIGURE 1. LVIS 3D mixed reality pipeline. The LVIS mixed reality pipeline converts generated 3D map information that is shown as 2D projections on the electroanatomic mapping system (EAMS) display (a) into 3D stereoscopic representations that appear suspended within the EP laboratory environment (b) Operators may walk or peer around the 3D model of real-time diagnostic mapping information, as if it were anchored in the real world (Simulated image). (c) 3D geometry, annotation and tool position data is exported from the EAMS to the LVIS server for format translation, encryption and compression, and is transmitted wirelessly to the Head mounted display (HMD). The HMD software renders the 3D data to the HMD stereoscopic display pipeline.",yes
PMC9375916,Figure_5,oa_package/bd/33/PMC9375916.tar.gz,"[' 5 A, B).', ' 5A, C).', ' 5D).', ' 5D).', ' 5E).', ' 5F I, the western blotting analysis further confirmed that the Torin 1 treatment markedly decreased the levels of phosphorylated p70S6K, phosphorylated Akt, and tau in PS1F105C/F105C neurons.', ' 5J).', '\n\nTorin 1 reduced tau phosphorylation and accumulation in human PS1 F105C mutant neurons.', '005 was considered significantly differentDiscussionDue to the difficulty to obtain cells from rare FAD PS1 mutant patients, gene editing tools are commonly used for generating human iPSC with FAD PS1 mutations.']","Fig. 5 Torin 1 reduced tau phosphorylation and accumulation in human PS1 F105C mutant neurons. Representative western blotting of tau, P-tau, and -Tubulin in PS1 , PS1 , and PS1 neurons. Tau and P-tau/tau were quantified by western blotting analysis (n=3). Anti-tau antibody can detect all six isoforms of tau. Data are represented as meanSEM. *P<0.05, and ***P<0.005. Tau immunofluorescence staining of neurons. The average intensity of tau in the soma was quantified (n50). Neurons were treated with 1 M Torin 1 or DMSO (vehicle control) for two days. Tau immunofluorescence staining of neurons with Torin 1 or DMSO treatment. The average intensity of tau in the soma was quantified (n50). Data are represented as meanSEM. ***P<0.005 was considered significantly different. Representative western blotting in PS1 , PS1 , and PS1 neurons. P-Akt/T-Akt, P-p70S6K/T-p70S6K, Tau, and P-Tau/Tau were quantified by western blotting analysis (n=3). Data are represented as meanSEM. ***P<0.005 was considered significantly different",yes
PMC10342683,Figure_3,oa_package/5e/22/PMC10342683.tar.gz,"['Diameter AnalysisThe significant changes in diameters are summarized in .', 'Aortic diameters analysis.']",Figure 3 Aortic diameters analysis. Aortic Diameters in mm at ( ) the aortic bifurcation and ( ) at the celiac trunk levels (Blue: T0; Green: T1; Khaki: T2). Evolution in mm of the aortic diameters at the aortic bifurcation level ( ) and at the celiac trunk level ( ) (Blue: aortic progression at 1 year; Green: aortic progression at the end of follow-up).,yes
PMC10505166,Figure_1,oa_package/83/a9/PMC10505166.tar.gz,"[' 1a.', ' 1b).', ' 1b) and analyzed with MS (Supplementary Table 1).', 'Antisense RNA from the C9orf72 mutation binds various proteins.', 'Following MS identification of interacting proteins, we performed RNA pull-down assays on protein lysates from human postmortem brain tissue to confirm the interactions.', ' 1b, c, Supplementary ', ' 1b, c, Supplementary ']",Fig. 1 Antisense RNA from the C9orf72 mutation binds various proteins. Schematic representation of the RNA pull-downassay and RNA constructs used in the experiments. Silver stained SDS-PAGE gels with labelled bands analyzed with mass spectrometry. The proteins were obtained with RNA pull-down assay performed on the nuclear and cytoplasmic fractions of mouse brain lysate. The RNA constructs 32C4G2-S1m and S1m were used. Results were repeated in two independent experiments. The identified proteins detected as interactors with western blot from the RNA pull-down assay performed on protein lysates from human postmortem brain tissue with 32C4G2-S1m and control RFP-S1m and S1m RNA constructs. Results were repeated in two independent experiments. Source data are provided as a Source data file.,yes
PMC3626254,Figure_3,oa_package/77/2c/PMC3626254.tar.gz,"['When the femoral capital epiphysis is completely\nseparate from the trochanteric apophysis, the femoral head is rounder\nand the neck is longer ().', '41,42 More commonly,\nhowever, the two physes are coalesced, resulting in a hip with a shorter,\nstouter neck and a smaller range of movement ().', '44Examples of separate and coalesced epiphyses\nduring development of the proximal femur.', 'Photographs and reconstructions showing\nthe evolution of the female pelvis, from the chimpanzee to man,\nin anteroposterior (AP) (top row) and axial views (bottom row) From\nleft to right: chimpanzee, Ardipithecus ramidus (4.']","Fig. 3 Examples of separate and coalesced epiphysesduring development of the proximal femur. At the end of growth,humans (A) have separation of the femoral capital epiphysis andthe trochanteric apophysis, resulting in a rounder femoral head andlonger femoral neck. Most quadripedal mammals (B) have coalescenceat the proximal femur, resulting in a shorter, stouter femoral neck,which is more stable but with a smaller range of movement. (Reprintedwith permission: Variation in mammalianproximal femoral development: comparative analysis of two distinctossification patterns. 2007;210:249258).",yes
PMC6181439,Figure_3,oa_package/d3/c8/PMC6181439.tar.gz,"['g003"">).', 'g003The bispecific antibody scPOM-bi binds simultaneously to GD and FT of PrP with high affinity.', 'g003""/>scPOM-bi binds with high affinity to globular domain and flexible tail of PrPSurface plasmon resonance (SPR) assays showed that scPOM-bi binds PrP with low nanomolar affinity, stronger than that of its individual single chain components due to avidity effects, resulting in slower dissociation, when the two antigen binding sites are engaged ().', 'Indeed, scPOM-bi was found to bind to both a PrP construct lacking the FT and to one lacking the GD with affinity similar to that of its individual components, scPOM1 and scPOM2 (), confirming that both paratopes of scPOM-bi correctly recognize their cognate epitopes.']",10.1371/journal.ppat.1007335.g003,yes
PMC3776555,Figure_1,oa_package/b4/b0/PMC3776555.tar.gz,"[', 2011) we detected anti-ceramide reactivity throughout the cytoplasm and in small clusters near the PM of NRK cells (A,B, control).', 'Permeabilization with the pore-forming toxin streptolysin O (SLO) had a similar effect, rapidly increasing the anti-ceramide reactivity at the cell periphery (A,B).', '10.', '003.', '00310.', '004 figure supplement 1.', 'Similar peripheral 80 nm endocytic vesicles were present in untreated cells, albeit in lower numbers (C).', 'Quantification revealed that treatment with SLO or SM for 30 s increased the number of BSA-gold-containing vesicles relative to controls (D).', 'At later time points (60 and 180 s) larger compartments suggestive of homotypic fusion of the 80 nm vesicles were increasingly observed (C).', 'Quantification of vesicle size, area and BSA-gold content supported the conclusion that the small endocytic vesicles induced by exposure to SLO or SM increase in size over time (E G).', ', 1999) (H,I).', 'Furthermore, transcriptional silencing of ASM reduced the number of peripheral 80 nm vesicles seen by TEM in cells exposed to SLO+Ca2+ ( figure supplement 1).', 'These results are fully consistent with our TEM analysis of SLO-permeabilized cells, showing an initial increase in the number of 80 nm endocytic vesicles followed by the gradual appearance of merged compartments (C).', 'No nuclear PI staining was detected in fibers treated with SM, demonstrating that exposure to this enzyme does not impair sarcolemma integrity (1).', 'As seen in C2C12 myotubes, treatment with SLO or SM resulted in increased numbers of intracellular caveolae-like vesicles along the sarcolemma, when compared to untreated controls (2A C).', 'Dissection resulted occasionally in localized fiber wounding (1 small arrows, 2D, 2 figure supplement 1), providing an opportunity to examine the effect of mechanical wounding in primary muscle fibers.', 'Intact regions along the fiber perimeter contained mostly a single layer of caveolae-like profiles close to the sarcolemma (2D panels 3 and 4 see whole data set in 2 figure supplement 1).', 'In contrast, an increased density of membrane profiles strongly resembling single and merged caveolae was evident in the proximity of wounds (2D panels 1 and 2, 2 figure supplement 1).', '10.', '0191.', '01910.', '0202.', '02010.', '0212 figure supplement 1.', 'The results are representative of several injured fibers observed in the two independent experiments described in 2.', 'Collectively, our results support a novel model proposing that injury to the PM triggers Ca2+ influx, exocytosis of lysosomes, ASM release and generation of surface-associated ceramide an event that facilitates caveolae internalization and lesion removal/repair (3A,B).', '10.', '0223.', 'We envision that the rapid intracellular merging of surface-connected caveolae, possibly enhanced by the higher levels of Ca2+ flowing through large lesions, might generate forces on the PM that constrict and ultimately reseal wounds (3B).', 'This model predicts that when the complex caveolar structures pinch off from the PM, large intracellular vesicles would be generated next to injury sites (3B).', '3) The authors should show that the very nice co-localisation between caveolin and SLO reported in D, E, F represents internalised SLO, not SLO that is in caveolae still connected to the plasma membrane.', '3) The authors should show that the very nice co-localization between caveolin and SLO reported in\nD, E, F\nrepresents internalised SLO, not SLO that is in caveolae still connected to the plasma membrane.']",10.7554/eLife.00926.003,yes
PMC6855557,Figure_4,oa_package/c2/9e/PMC6855557.tar.gz,['.'],"Figure 4. Gelfoam setup showing syringes connected by a (A) 3-way valve, (B) intact Gelfoam, and (C) prepared Gelfoam cut for endoscopically deliverable slurry.",yes
PMC11591949,Figure_3,oa_package/db/82/PMC11591949.tar.gz,"['The effect of macrophage status alteration has also been studied in various heterogeneous settings ().', 'On the other hand, inflammatory stimulation of macrophages may also be responsible for PDGF-dependent profibrotic activation of cardiac MSCs, giving rise to myofibroblasts that sustain fibrosis in experimental MI [131] ().', 'In this case, the M2 macrophages promote proliferation, migration, collagen expression, and myofibroblast trans-differentiation, acting beneficially by augmenting repair following MI ().', 'OPN and IL1 were suggested to be the mediators acting downstream of IL4 in that case, as they were secreted by IL4-polarized macrophages and induced myofibroblast trans-differentiation sustaining cardiac repair ().', 'In another approach, where BMDMs were M2 polarized by IL4 exposure, the extracellular vesicles (EVs) were isolated from the macrophage secretome and administered to mice, leading to increased fibrosis and augmented dysfunction early post MI ().', 'In this setting, the fibroblasts were pre-exposed to hydrogen peroxide (H2O2), mimicking ischemic conditions in MI, and in contrast to the M2 (IL4)-activated macrophages, LPS-exposed proinflammatory macrophages exerted opposite effects, protecting the fibroblasts against apoptosis ().', 'Although direct cardiac injection of M1 macrophages in rat infarcts was shown to promote fibrosis () [137], the secreted mediators of these differential effects following distinct macrophage activation regimes have not been fully elucidated.', 'The underlying mechanism involves binding of neuregulin to its receptors (Erb2 and Erb4) on the cell surface of fibroblasts and ERK/Akt signaling activation ().', 'This allowed the prevalence of an M1-like phenotype by Ly6Chi monocyte-derived macrophages infiltrating the infused hearts, which when co-cultured with cardiac fibroblasts, induced Acta2 (encoding SMA) expression and myofibroblast trans-differentiation of the latter ().', '7 cells inhibits cardiac fibroblast migration (), likely explaining the increased post-MI repair observed in Trim21 / mice [140].', 'Overall, macrophage activation status differentially affects fibroblast responses during cardiac repair and fibrosis () and merits systematic study efforts to fully reveal the specific mediators involved that could form an arsenal for future intervention studies.', 'Macrophage status contributions to fibroblast-mediated cardiac repair and fibrosis.']","Figure 3 Macrophage status contributions to fibroblast-mediated cardiac repair and fibrosis. The in vivo and in vitro induction of the so-called M1 or M2 (and subcategories) macrophage activation affects the cardiac outcome in myocardial infarction (MI) or stress-induced (AngII) cardiomyopathy and HF models. In some cases, the pathways affected, and the mediators secreted by M1- or M2-activated macrophages that regulate in a paracrine manner cardiac fibroblast activation, differentiation, migration, proliferation, senescence, and survival, have been identified. Protocols using ex vivo activation and direct heart injection of macrophages have been applied to animal models (top right) leading to either beneficial repair or detrimental fibrosis. cFbscardiac fibroblasts; MyoFbsmyofibroblasts; MSCsmesenchymal stem cells. Arrows with headsinduction; arrows bluntedinhibition. For the rest of the abbreviations and details, see text. Created with BioRender.com.",yes
PMC7319832,Figure_1,oa_package/6a/c0/PMC7319832.tar.gz,['Primary emergency coronary angiography.'],Figure 1 Primary emergency coronary angiography. ( ) Severe stenosis of the distal left main coronary artery (white arrow) and occlusion of the mid left anterior descending (black asterisk). ( ) Result of the left main percutaneous coronary intervention. ( ) Contrast extravasation following balloon inflation (circle shows the contrast extravasation). ( ) Final result with suspected coronary cameral fistula (circle shows the contrast extravasation into a heart cavity).,yes
PMC4598538,Figure_2,oa_package/ba/c1/PMC4598538.tar.gz,"['(b) Oblique type of proximal tibiofibular jointA line diagram showing (a) Anterolateral dislocation of the proximal tibiofibular joint.', '6 Anterolateral or Type II dislocation is by far the most frequent variety, about 85% [a] and often associated with ligamentous injury and peroneal nerve palsy.', 'Type III injury [b] is associated with a transient common peroneal nerve injury whereas, Type IV dislocation [a] is rare and is most often associated with common peroneal injury and usually caused by high energy ankle injuries.']",Figure 2 A line diagram showing (a) Anterolateral dislocation of the proximal tibiofibular joint. (b) Posteromedial dislocation of the proximal tibiofibular joint,yes
PMC7279687,Figure_3,oa_package/db/09/PMC7279687.tar.gz,['CT-guided drainage of mediastinal hematoma with an 8.'],Figure 3 CT-guided drainage of mediastinal hematoma with an 8.5-French pigtail catheter.,yes
PMC4231287,Figure_3,oa_package/9f/e9/PMC4231287.tar.gz,"[' 3b]).', 'Table 1: CDR-Ab effects in percentage\nWestern blot analysisThe treated cOTSC were washed with pre-warmed 1xPBS (pH 7.', ' 3a, merged).', ' 3b).', ' 3c).', ' 3c, d).', ' 3d; Table 1).', ' 3d; Table 1).', ' 3e) with no difference between rCDR2 and rCDR2L.', ' 3f, g).', ' 3h, i).', ' 3i).', ' 3h, i; Table 1), but plateaued between days 4 and 7 at ~ 40 % CB+-PC loss before reaching the rIgG control count.', ' 3a showed no cell loss.']","Fig.3 Affinity-purified rabbit CDR-Abs ( CDR) reduce calbindin D immunoreactivity in a time- and concentration-dependent manner with partial recovery after CDR-Ab washout. CDR2 and CDR2L (400ng/mL, 6D) are independently internalized into PCs ( ). Only few CDR-marked cells ( ) are positively correlated with calbindin D (CB, ); 20m. We found different expression patterns of CB immunoreactivity and PC morphology modifications for CDR2 , CDR2L, and CDR2/2L compared to IgG control ( ); 40m. The CB immunoreactivity loss, studied 2, 4, and 6days of CDR internalization by stereological counting of CB cells/mm , was time- ( ) and concentration- ( ) dependent (time: =4; concentration: =3). , Western blot analysis of CDR-treated cOTSC lysate (125ng/mL, D6) showed no difference in CB protein levels to IgG controls ( =10). CB -PC immunoreactivity loss at day 6 of CDR internalization was partially rescued 7days (D7wo) post- CDR washout ( 40m), but did not reach control level and plateaued between washout days 4 and 7 ( =3) ( ). Data in meanSEM. Non-parametric two-tailed paired MannWhitneys test. * <0.05; ** <0.01; *** <0.001. Table : CDR-Ab effects in percentage",yes
PMC11628733,Figure_3,oa_package/01/67/PMC11628733.tar.gz,[],FIGURE 3 Xray of a 77yearold female with Morgagni hernia.,yes
PMC10314548,Figure_2,oa_package/08/83/PMC10314548.tar.gz,"['The skin biopsies demonstrated lichenoid dermatitis with eosinophils and focal subepidermal clefting (figure 2).', 'Low magnification of H E stain showing lichenoid dermatitis with focal subepidermal clefting (top).']",Figure 2 Low magnification of H&E stain showing lichenoid dermatitis with focal subepidermal clefting (top). High magnification of H&E specimen showing lichenoid dermatitis with eosinophils (bottom).,yes
PMC9692034,Figure_1,oa_package/f8/25/PMC9692034.tar.gz,"['In these cases, the vacuoles were localized to the INL and were associated with transudation of intraretinal and subretinal fluid originating from the optic disc (see for 1 example).', 'The microcystic change in these cases was transient and had resolved in all cases at 1 month ().', ', ).', ' 1Spectral-domain OCT (Heidelberg Engineering) imaging.', 'These microcystic changes were observed in the peripapillary region and were associated with fluid localized between the ellipsoid zone and the outer plexiform layer in the parafoveal region ().']","Figure1 Spectral-domain OCT (Heidelberg Engineering) imaging. , Microcystic change visible in the peripapillary region in moderate swelling of the disc (cohort case; blue arrows). , Transudation of fluid through the retina at presentation. , Twenty-three days later in 1 case selected from the 19 out 34 cases showing disc change without subsequent retrograde maculopathy (cohort case).",yes
PMC8446506,Figure_1,oa_package/fb/f0/PMC8446506.tar.gz,"['Compared with ND-fed rats, body weight of HD rats was low at the end of 16 weeks of feeding (C).', 'Body weight and renal pathology analysis of ND and HD rats (A) food intake, (B) body weight gain, and (C) body weight.']","Figure 1 Body weight and renal pathology analysis of ND and HD rats food intake, body weight gain, and body weight. * < 0.05 vs. ND. The data are presented as mean SD ( = 6/group) H&E stained images of kidney from ND and HD ( = 3/group). The images are presented at 40x magnification. ND, Normal diet fed; FD, High fat diet fed.",yes
PMC10640650,Figure_3,oa_package/d4/2b/PMC10640650.tar.gz,"['3A).', '3B, E), and the astrocyte marker protein, GFAP, in two different regions (', '3C, F).', '3A showed that WT mice were significantly enriched in microglia and astrocytes in the lesion area after completion of CPZ modeling and gradually returned to the resting state over time (', '3A).', '3A), when we found that microglia and astrocytes normalized in WT mice, but showed an abnormal increase in Fkbp5ko mice, a trend that was consistent with all stages of myelin loss.', 'FKBP5 deletion resulted in delayed microglia and astrocyte enrichment processes.', 'High concentrations free radicals have been found in inflammatory MS lesions [31].', '3D, G), we were surprised to find that both mice genotypes showed a peak in superoxide anion after one week of recovery.']","Fig. 3 FKBP5 deletion resulted in delayed microglia and astrocyte enrichment processes. Fluorescence staining maps showing changes in microglia (red) and astrocytes (green) in the corpus callosum and surrounding tissue regions of brain tissue in WT and Fkbp5 mice at 0, 1, and 3 weeks after induction of demyelination. Two regions were identified as glial cell enrichment areas by low magnification observations. The lateral horn of the ventricle is located in the region of the corpus callosum (shown by the dashed box) and the central region of the coronal section of the corpus callosum (dashed oval box), and both are framed in the region of the corpus callosum at high magnification. , , , Histograms of the number of positive microglia and astrocytes at the two locations, with the two arms in the downward column and the central location in the upward column. , Equal masses of fresh tissues from each group were cut up, treated with trypsin, and then detected at two wavelengths of 420 and 600nm for superoxide anion (O ) content and counted, respectively.",yes
PMC7683091,Figure_2,oa_package/6e/f0/PMC7683091.tar.gz,['Cell origin identification of TSPO overexpression in the hippocampus of TgF344-AD rats.'],"Fig. 2 Cell origin identification of TSPO overexpression in the hippocampus of TgF344-AD rats. The [ I]CLINDE concentrations were determined in 24-month-old wild-type animals (WT) and in 12- and 24-month-old (12m and 24m) TgF344-AD rats. A) Radioactivity was determined in GLT1 (astrocytes), CD11b (microglia), and CD31 (endothelial cells) cell population (% injected dose/g of tissue). B) Radioactivity per cell (% injected dose/cell) in each cell population. C) number of cells sorted. [ I]CLINDE concentrations and cell number were analyzed by two-way ANOVA. tests indicate genotype differences () and age effects in TgF344-AD (). The number of symbols indicates the significance level (1: <0.05; 2: <0.01; 3: <0.001). MeanSEM of 8 animals per genotype and per age are presented.",yes
PMC7463502,Figure_4,oa_package/ca/50/PMC7463502.tar.gz,"['SkQ1 Restores the Expression of Genes Associated with the p38 MAPKsp in the Hippocampus of OXYS RatsSupplementation with SkQ1 changed the DEGs and their total number, among those that are known to be involved in the p38 MAPKsp (a).', 'Supplementation with SkQ1 normalized the expression of genes Irf1, Mapk14, and Traf2 (whose mRNA levels were high in untreated OXYS rats; b) and increased the expression of genes Dusp1, Mapk8ip2, and Mapkapk3.', 'The influence of supplementation with SkQ1 in OXYS rats on the number of DEGs among those known to be involved in the p38 MAPKsp (a).']",Figure 4 The influence of supplementation with SkQ1 in OXYS rats on the number of DEGs among those known to be involved in the p38 MAPKsp ( ). The Venn diagram depicts overlapping sets of DEGs in control and SkQ1-treated 18-month-old OXYS rats compared of untreated Wistar rats ( ).,yes
PMC11657929,Figure_1,oa_package/e7/b7/PMC11657929.tar.gz,[],"FIGURE 1 Generation of a novel . panneuronal overexpression strain. (a) Schematic representation of the :: construct. The nematode (yellow) is fused to (green) and expressed panneuronally driven by the promoter (gray). (b) Confocal fluorescent images of young adult (day 4) ( and ) . strains. Two strains were selected during plasmid integration: strain on the right shows 1.7fold higher expression than the on the left. Images are 100fold magnified (scale bar is 200m). Insets 13 show the respective closeups of the head region (1), the midbody (2), and the tail (3) for the respective strains. Closeup images are 400fold magnified (scale bar is 50m). Further experiments were performed using . A scatter dot blot of HSP110::GFP quantification of and by confocal imaging is depicted below. Fluorescence intensity values are displayed relative to . Data are displayed as mean intensity SD. (c) Western blot detecting GFP. Soluble fractions of N2 wildtype and (day 4) lysates were analyzed using mouse antiGFP B34 antibodies (Enzo). Only one protein band is visible in the strain with the correct molecular weight (see arrow). The molecular weight marker was visualized separately and combined with the GFP chemiluminescent signal. (d) Representative western blot detecting HSP110. On the left, total lysates I and N2 wildtype (day 4) were used to detect HSP110 using rabbit antinematode HSP110 antibody. Endogenous HSP110 (100kDa) is visible in both and N2 wildtype animals (see arrow). Neuronal HSP110::GFP is detected as a slower migrating protein band only in the strain (see arrow). Signal intensities were normalized to tubulin. On the right, quantification of the western blot signals of total HSP110 in N2 wildtype and . The molecular weight marker was visualized separately and combined with the HSP110 chemiluminescent signal. Total HSP110 abundance (endogenous and overexpressed GFPtagged HSP110) was quantified and normalized to tubulin. Three biological replicates of total lysates of N2 wildtype and (day 4) were analyzed. The graph depicts the relative abundance of total HSP110 of (green bar) to N2 wildtype. Data are displayed as mean intensity SD. The strain results in 135% HSP110 expression compared to wildtype. (e) Quantification of HSP110::GFP fluorescence with the progression of aging of the strain. Confocal fluorescent images of three cohorts of 1013 nematodes each were analyzed on day 4, day 7, and day 10 of life. Fluorescence intensities were quantified by Fiji. The images were corrected for the increase in autofluorescence with aging by subtracting the fluorescence intensities of agematched N2 wildtype nematodes. Fluorescence intensities were normalized to day 4. The graph shows an increase in HSP110::GFP level with aging (day 7 and day 10). Data are displayed as mean fluorescence intensity SD (graph and table). Significance was tested by oneway ANOVA + Bonferroni post hoc test (****= <0.0001).",yes
PMC11351141,Figure_2,oa_package/69/f6/PMC11351141.tar.gz,"['To test the expression and kinetics of NRF2 protein translation, a lung epithelial cell line (BEAS-2B) was transfected with the construct, and NRF2 protein expression was assessed between 6 and 48 h (A).', 'Maximal HMOX1 transcript upregulation was observed in BEAS-2Bs at 6 h post-transfection, and by 24 h, it had returned to baseline for all hNRF2 cmRNAs (B).', '2-fold at 24 h (C).', 'A dose-dependent increase in activity was observed with all hNRF2 cmRNAs and Compound 7 (D).', 'Compound 7 treatment resulted in significant luciferase expression at all dose levels tested (E).', 'Functional NRF2 expression from hNRF2 cmRNA in human cell lines.']",Figure 2 Functional NRF2 expression from hNRF2 cmRNA in human cell lines. ( ) NRF2 protein expression following transfection of hNRF2 cmRNAs in BEAS-2B cells. The full Western blot images were cropped to the relevant NRF2 band. ( ) HMOX1 gene expression following transfection of hNRF2 cmRNAs in BEAS-2B cells. Data are pooled results from 3 independent experiments showing mean SD. * < 0.05; ordinary one-way ANOVA with Dunnetts multiple comparisons against eGFP-transfected control at each timepoint. ( ) Table detailing mean values from ( ) and SD. ( ) ARE-luciferase values 24 h after transfection of hNRF2 cmRNAs in ARE-luc HepG2 cells. * < 0.05; ordinary one-way ANOVA with Dunnetts multiple comparisons against 5.3 ng/cm eGFP-transfected control. ( ) Table detailing mean maximum luciferase signal for each treatment group in ( ) and SD.,yes
PMC10539866,Figure_3,oa_package/5e/fd/PMC10539866.tar.gz,[],Supplementary Fig 1 MRI coronal knee slices at the extreme anterior (left image) and extreme posterior (right image). Meniscus identification on these slices is rather difficult.,yes
PMC10545147,Figure_2,oa_package/8a/3d/PMC10545147.tar.gz,"['Pathology testing revealed nasal mucosa with zonal necrosis surrounded by polymorphous infiltrate of large atypical lymphocytes, immunoblasts, small lymphocytes and plasma cells (, 3).']",Figure 2 Low power hematoxylin and eosin-stained slide at 100 with the upper portion showing zonal necrosis (black) and the lower portion of the picture showing large atypical cells (red),yes
PMC5540284,Figure_8,oa_package/1a/69/PMC5540284.tar.gz,"['41; ), indicating that species with more extensive UV fluorescence tend toward multiple concurrent WNS findings on histopathology.', 'g008Increase in weighted cumulative white-nose syndrome pathology score with progressing UV fluorescence.', 'For this chronic skin infection, severity-grading scores were understandably higher in bats with more extensive UV fluorescence ().']",10.1371/journal.pone.0180435.g008,yes
PMC6927526,Figure_9,oa_package/e9/a0/PMC6927526.tar.gz,[],"Figure 2. T cell derived TNF is critical for induction of pro-IL-1 in BMDCs (a) lL-1, as quantified by ELISA, in the supernatants of WT T 0 cells co-cultured with WT or BMDCs in the presence of CD3 Ab for 6 h. Error bars indicate SEM for n=4 independent experiments. (b) IL-1 measured by ELISA following 6 h of culture with WT T 0 cells and WT BMDCs in the presence of CD3 Ab and neutralizing TNF Ab (20g/mL), FasL Ab (10g/mL), or CD40L Ab (20g/mL). Error bars indicate SEM for n=3 independent experiments. (c) Immunoblot analysis of pro-IL-1 (p35) in the lysates of WT BMDCs stimulated with LPS (100ng/mL) or cultured with T 0 cells in the presence of CD3 Ab and neutralizing antibodies. Data are representative of two independent experiments. (d) Expression of intracellular pro-IL-1 measured by flow cytometry in WT live, CD90 CD11c BMDCs cultured with T 0 cells in the presence of CD3 Ab and neutralizing TNF Ab (20g/mL) for 6h. Flow plots are representative of four independent experiments. Error bars indicate SEM for n=4 independent experiments. (e) Expression of intracellular TNF measured by flow cytometry in WT T 0 cells (left; live,CD11c CD90.2 ) and WT BMDCs (right; live,CD90.2 CD11c ) that were co-cultured for 3 h in the presence of CD3 Ab and brefeldin A. Data are representative of two independent experiments. (f) Expression of intracellular TNF measured by flow cytometry in effector CD4 T cells (live,CD11c CD90.2 ) polarized to T 1, T 2 and T 17 lineages cultured with WT BMDCs in the presence of CD3 Ab and brefeldin A for 3h. Cells were considered to be transcription factor positive based on isotype control antibody staining. Data are representative of three independent experiments. (g) Immunoblot analysis of pro-IL-1 (p35) in the lysates of WT or T 0 cells cultured WT BMDCs in the presence or absence of CD3 Ab for 6h. Data are representative of two independent experiments. (h) lL-1 was quantified by ELISA in the supernatants of WT T 0 cells co-cultured with WT or BMDCs in the presence of CD3 Ab for 6 h. Error bars indicate SEM for n=4 independent experiments. (a, b, d, h) Statistical analysis was performed by paired, one-tailed Students -test. <0.05, ** n.s.=not significant.",yes
PMC7660907,Figure_6,oa_package/03/cd/PMC7660907.tar.gz,"['s003"">S3 Fig), or at 1 and 24 hours post-infection (A), we found that dermal TRMs constitutively express Tim-4 and Mertk, which were not modulated by L.', 'in comparison to the wild type (WT) mice, nor were the numbers of LmRFP+ neutrophils, inflammatory monocytes, or mo-DCs significantly changed (B).', 'However, the number of LmRFP+ dermal TRMs was significantly decreased by roughly half in the Axl-/-Mertk-/- mice (B), which was associated with a small reduction in the total number of dermal TRMs in the injection site at 24 hr p.', 'g006Role of TAM RTKs in infection and M2 polarization of dermal TRM during L.', 'in WT mice, 9% and 32% of these cells, respectively, stained positive for Relm (C).', 'uninfected dermal TRMs (D).', 'In the Axl-/-Mertk-/- mice, the frequency of Relm + cells and the Relm expression levels were also significantly higher in the infected compared to uninfected dermal TRMs recovered from the same site (C and 6D).', 'These parameters, however, were in each case significantly reduced compared to WT mice (C and 6D), resulting in a significant decrease in the frequency of Relm + cells in the total dermal TRM population in the Axl-/-Mertk-/- mice (E).', 'A similar consequence of the Axl/Mertk deficiency was observed with regard to the expression of Arg1 on dermal TRMs from infected mice, which was confined to infected cells, and reduced in frequency on infected cells from the Axl-/-Mertk-/- mice (F).', 'The reduced frequency of Arg1+ cells in the population of infected, dermal TRMs from Axl-/-Mertk-/- mice was associated with a significantly increased frequency of iNOS+ cells in this population (G).', 'Our findings confirm the expression of Mertk on dermal TRMs from na ve mice, and show that Axl is expressed on dermal TRMs only after 24 hr and by 10 days post-infection (A and .']",Figure 3. Axial ( ) and coronal ( ) -weighted rectal MRI images demonstrating a small Richter type hernia of the left lateral rectal wall (red arrow) through a 22mm defect in the left levator ani muscle (yellow arrow). No restricted diffusion is noted on ADC ( ) and DWI ( ) axial sequences in the region of the perineal hernia (yellow circles).,yes
PMC9327202,Figure_6,oa_package/3b/55/PMC9327202.tar.gz,"[' 6a c; 6 months: ', ' 6d f).', ' 6a c; p 0.', ' 6d f).', ' 6g i).', ' 6j l).', ' 6g i, j l), indicating that reduction of A did not ameliorate the existing astrocytic phenotype inherent in the Abi3-Gngt2 line.', 'Abi3-Gngt2 regulates gliosis in APP mice.', '05We performed bulk RNAseq from forebrains of 3-month-old TG-Abi3-Gngt2 mice (']","Fig. 6 - regulates gliosis in mice. Representative images of Iba-1 reactive microglia ( ) and GFAP-reactive astrocyte ( - ) in 3-month-old or 6-month-old transgenic mice with WT (+/+), heterozygous (+/), or KO (/.) of locus. Quantitation of the Iba-1 or GFAP staining from cortex or hippocampus is provided in corresponding panels on the right side. Scale bar, 50m. Clear symbols denote female mice and filled symbols denote male mice. =3 females, 3 males (3months) and =3 females, 4 males (6months). Data represents meansem. One-way ANOVA; *** <0.001; ** <0.01; * <0.05",yes
PMC3512286,Figure_7,oa_package/ac/eb/PMC3512286.tar.gz,"['LS have been proven to be useful in visualizing gastrointestinal tumours [46] ().', '006""/>Large format section from an intestinal adenomatous polyp with adenocarcinoma.']",Figure 7 Large format section from an intestinal adenomatous polyp with adenocarcinoma. The level of invasion by the adenocarcinomatous area is easily assessed.,yes
PMC4405913,Figure_2,oa_package/24/8a/PMC4405913.tar.gz,['Core needle aspiration was not performed because the lesion was so consistent with liposarcoma in MRI.'],"Figure 2 The well-circumscribed multinodular mass with heterogeneous intensity of this retroperitoneal liposarcoma. Star for tumor, triangle for gelatinous components (2).",yes
PMC10667107,Figure_7,oa_package/69/93/PMC10667107.tar.gz,"['1a c).', '1k m).', '1e) and C3 (expressed by both astrocytes and microglia; Extended Data ', '1f,g) with corticostriatal synapses in the caudate nucleus of HD brains.', '1c,d,o).', '1h).', '1i,j), consistent with a previous study that used unbiased transcriptomic profiling in the BA9 region of premanifest HD patients55.', '1k m).', '1n).', '1n, should not be unexpected.', '1k,l); and that it was selective for iC3b over native C3 and other C3 cleavage fragments (Extended Data ', '1m).', '/Table 7Source data for graphs and statistical analysis associated with Extended Data .', '\nExtended data\nExtended Data Loss of specific synaptic populations, activation and association of complement proteins with synaptic elements and adoption of a more phagocytic microglial state are evident in postmortem brain tissue from HD patients.', '\nSource data\n\n\nExtended Data Early and selective loss of corticostriatal synapses in a mouse model of Huntington s disease.', 'Suri) for their provision of the C1q function-blocking antibody as well as the measurements of C1q-blocking antibody and unbound C1q levels documented in Extended Data c,d and, also, helpful discussions.', '1 has associated raw data for the immunoblots that can be located in Source Data Figs.']","Extended Data Fig. 1 Loss of specific synaptic populations, activation and association of complement proteins with synaptic elements and adoption of a more phagocytic microglial state are evident in postmortem brain tissue from HD patients. Bar chart showing quantification of VGLUT1 immunoreactive puncta in the caudate nucleus of control, Vonsattel grade 2 HD and Vonsattel grade 4 HD tissue, n=6 control, n=4 HD with Vonsattel 2 tissue grade and 4 HD with Vonsattel 4 tissue grade. One way anova p=0.0005; Tukeys multiple comparisons test, control vs HD2 p=0.0138; control vs HD4 p=0.0004; HD2 vs HD4 p=0.172. Bar chart showing quantification of HOMER1 immunoreactive puncta in the caudate nucleus of control, Vonsattel grade 2 HD and Vonsattel grade 4 HD tissue, n=6 control, n=4 HD with Vonsattel 2 tissue grade and 4 HD with Vonsattel 4 tissue grade. One way anova p=0.0054; Tukeys multiple comparisons test, control vs HD2 p=0.0442; control vs HD4 p=0.0058; HD2 vs HD4 p=0.541. Representative confocal image showing staining for corticostriatal synaptic markers in the caudate nucleus of an HD patient, who has been assessed to be Vonsattel grade 4. Scale bar = 5m. This experiment was repeated 3 times. Left panel is a representative confocal image showing staining for C1Q and VGLUT1 in the caudate nucleus of an HD patient, who has been assessed to be Vonsattel grade 4. Scale bar = 5m. Right panel is a representative confocal image showing staining for C3 and VGLUT1 in the caudate nucleus of an HD patient, who has been assessed to be Vonsattel grade 4. Scale bar = 5m. This experiment was repeated 3 times. Representative confocal image of in situ staining for and alongside IHC for microglial marker IBA1 in the caudate nucleus of postmortem tissue from an HD patient (Vonsattel grade 2). Scale bar = 20m. Representative confocal image of in situ staining for alongside IHC for microglial marker IBA1 in the caudate nucleus of postmortem tissue from an HD patient (Vonsattel grade 2). Scale bar = 20m. This experiment was repeated 3 times. Representative confocal image of an in situ staining for alongside IHC for astrocytic marker S100 in the caudate nucleus of postmortem tissue from an HD patient (Vonsattel grade 2). Scale bar = 20m. This experiment was repeated 3 times. Bar chart showing ELISA measurements of the concentration of complement receptor CR3 in proteins extracted from the globus pallidus (GP) of postmortem tissue from manifest HD patients and control (no documented evidence of neurodegenerative disease; see and supplemental table ) individuals after normalization for total tissue homogenate protein content, n=4 control GP and 6 HD GP (there was not enough protein available from one of the control sample that had previously been employed in the C3, iC3b and hemoglobin ELISAs (the results of which are depicted in Fig. and ) and as such it was not tested here). Unpaired two-tailed t-test p=0.096 Dot plot showing the fold change in mRNA levels of complement component C3 in samples from the caudate nucleus of two premanifest HD patients relative to those seen in two clinically normal (see and Supplemental table ) individuals. Dot plot showing the fold change in mRNA levels of complement receptor CR3 in samples from the caudate nucleus of two premanifest HD patients relative to those seen in two clinically normal (see and Supplemental table ) individuals. Superimposed scatter blot showing the relative absorbance values of different serum samples employed in the iC3b ELISA. Note that in two independent serum samples, in which the complement pathway has been activated either by incubating the serum at 4C for 7 days or treating with 10mg/ml of zymosan for 30min at 37C, the absorbance values for the highest concentration of serum tested are approximately double that of the same samples left untreated and maintained at -80C. Thus demonstrating that this assay reflects complement cascade activation as would be predicted for an ELISA measuring levels of iC3b, a cleavage fragment of complement component C3 formed following cascade activation. Bar graph showing the relative absorbance values of C3/C4 inactivated serum samples employed in the iC3b ELISA. Note that, unlike in treatment of this serum with zymosan fails to increase levels of iC3b. Thus confirming the specificity of the ELISA by demonstrating that it reflects changes in a species that increases in response to complement cascade activation but is prevented from forming in the absence of functioning C3 and C4. Superimposed scatter blot showing the relative absorbance values of different concentrations of complement component C3 standards purified from human serum using PEG precipitation and DEAE ion chromatography. Note that at all concentrations tested iC3b (generated by the cleavage of C3b with factor I in the presence of factor H) gave a higher absorbance value than uncleaved full length C3 or a subsequent cleavage component C3c (generated by treating iC3b with trypsin like proteases). Thus further demonstrating the specificity of this ELISA for iC3b versus the full-length protein or other cleavage components. Representative images of non-transfected HEK 293 cells or those transfected with pULTRA EGFP or pULTRA EGFP T2A C3Ms stained with the same C3 antibody employed in the immunohistochemical analysis depicted in this figure and in Fig. , Extended Data Fig. , Fig. , Extended Data Fig. , and Extended Data Fig. . Scale bar = 100m. Orthogonal view of a representative structured illumination image showing C3 and VGLUT1 staining in the caudate nucleus of tissue from an HD patient (Vonsattel grade 4). Scale bar = 2m. For bar charts, bars depict the mean This experiment was repeated four times. All error bars represent SEM. Stars depict level of significance with *=p<0.05, **p=<0.01 and ***p<0.001.",yes
PMC7186102,Figure_1,oa_package/48/57/PMC7186102.tar.gz,['A 45-year-old woman with COVID-19 infection.'],Figure 1 A 45-year-old woman with COVID-19 infection. A noncontrast-enhanced CT image of the lungs demonstrating bilateral patchy ill-defined ground-glass opacities (GGOs) in posterior segments of the lungs (A) and an inset magnified view of the lesion for better delineation at the location of GGO in the right lower lobe of the lung (B).,yes
PMC11101445,Figure_4,oa_package/eb/30/PMC11101445.tar.gz,"['Finally, for the sets of nodules annotated and identified in the medical reports, the Fleischner score was used, categorizing each exam into four classes: 1) No routine follow-up required or optional CT at 12 months according to patient risk; 2) CT at 6-12 months required; 3) CT at 3-6 months required; and 4) CT, Positron Emission Tomography (PET), or tissue sampling at 3 months required.']",Fig. 4 Example of some mismatched nodules.,yes
PMC7493419,Figure_3,oa_package/08/b7/PMC7493419.tar.gz,"[' 3).', 'No image manipulation was performedDiscussion and conclusionsAlthough it is a rare entity, approximately 181 cases have been reported in the literature, equally affecting males and women and in all age groups.', '3, 4).']","Fig. 3 , . Coloring of H&E ovoid structures denatured red blood cells and covered by a thicker membrane, negative for epithelial material, magnification 20x y 40x . Images were obtained using the Ventana system from Medical Systems Iscan Slide Scanner. Automated barcode reading, tissue identification, scanning and image compression and generation. Scan viewing 24-bit true color. No image manipulation was performed",yes
PMC6130068,Figure_4,oa_package/9e/b1/PMC6130068.tar.gz,"[' 4 and corroborate the differences described above.', ' 4g) shows the number of intersections per distance from the cell soma.', 'Morphological Sholl analysis of mRGCs in PD and controls.', 'DiscussionIn recent years, a huge effort has been made to study the state and health of brain regions related to circadian rhythms disturbances and sleep dysregulation, like the suprachiasmatic nucleus, but these alterations are not yet completely understood and some regions are found not to be affected until advanced states of the disease [22].']","Fig. 4 Morphological Sholl analysis of mRGCs in PD and controls. , Comparison of terminal points number per cell in PD and controls considering total mRGC ( ) or per cell type ( ). , Comparison of the number of branch points in all mRCGs ( ) and per cell type ( ) in PD and controls. , Sholl area comparison of PD and control mRGCs globally ( ) and per cell type ( ). Data is presented as mean s.d. * <0.05, ** <0.01, *** <0.001, **** <0.0001. Sholl analysis curve representing number of intersections per distance from soma, comparing total controls and PD mRGCs. Data is presented as mean s.e.m.",yes
PMC9485637,Figure_6,oa_package/ca/1b/PMC9485637.tar.gz,"['The module allows for the simple and rapid visualization of structures such as septal defects, valve leaflets, and the vasculature of patients from 3DE ().', 'Volume rendering of 3D echocardiographic and tomographic data.']","Figure 6 Volume rendering of 3D echocardiographic and tomographic data. Visualization of an atrial septal defect in the echo volume render module in SlicerHeart from a right atrial view; Visualization of a complete common atrioventricular canal valve in diastole (ventricular view in SlicerHeart); Left atrial view of an aortic valve and a mitral valve with anterior leaflet prolapse and a ruptured chord which is visible as indicated by the red arrow; CT image of a normal anatomic heart cropped anteriorly to visualize the right atrium and ventricle and the left ventricle using volume rendering in 3D Slicer. CT, Computed Tomography.",yes
PMC9807517,Figure_2,oa_package/a2/a7/PMC9807517.tar.gz,[],"FIGURE 2 Dual reactivity of antidensely sulfated glycosaminoglycan (antidsGAG) clones to nucleic acids (NAs). A, Schematic diagram of heparin (dsGAG) and phosphodiester backbone of DNA. B, doublestranded DNA (dsDNA) ELISA signals of tumorinfiltrating dominant clones ( =3). C, dsDNA ELISA signals of the highaffinity antidsGAG clones with/without coincubation of Heparin ( =3). Serum from autoimmune patients were used as a control. D, E, ELISA signals of highaffinity antidsGAG clones to 6mer oligos of singlestranded DNA (ssDNA) (D) and ssRNA (E) ( =2). F, G, Signals of ssDNA (F) and ssRNA (G) ELISAs with/without coincubation of heparin ( =2). H, dsDNA and ssDNA ELISAs were performed in parallel for highaffinity antidsGAG clones ( =2). I, Defined antigens (Ags) for 20 out of 30 reconstructed tumorinfiltrating dominant immunoglobulin (Ig) clones with the information of their proportions of lineage sizes in the repertoires in each tumor. J, Internalizations of phycoerythrin (PE)ssDNA and FITCheparin with/without coincubations of #022T2 were evaluated by flow cytometry (FC) using THP1 cell. K, TLR9 signals in THP1 cell with/without coincubations of ssDNA and/or #022T2 were evaluated by NfkB and IRF reporter systems ( =3). L, Activations of TLR7/9 in HEKBlue cells were evaluated with/without coincubation of R848 (hTLR7 agonist), ODN2006 (hTLR9 agonist), or heparin ( =2). Bars and error bars represent the mean and SD, respectively.",yes
PMC10564677,Figure_7,oa_package/3d/4d/PMC10564677.tar.gz,"[' 7a).', ' 7b, c).', 'FAPs demonstrate a shifted collagen homeostasis with potential consequences for muscle health in IBM.', 'CDKN1A cyclin dependent kinase inhibitor 1A; COL collagen; FAP fibro-adipogenic progenitor; NDC non-diseased control; IBM inclusion body myositis; IMNM immune-mediated necrotizing myopathy; UMAP uniform manifold approximation and projectionFollowing this line of argumentation, we suspected that differences in collagen might affect skeletal muscle cell homeostasis.', ' 7d).', ' 7e).', ' 7g, h).']","Fig. 7 FAPs demonstrate a shifted collagen homeostasis with potential consequences for muscle health in IBM. UMAP embedding of the full dataset with the corresponding gene expression indicated by the colour code. The FAP cluster is in the upper left. UMAP of the FAP subclusters split into their origin of IBM patients or NDC. The annotation of the corresponding subcluster is given beside the plot. CDKN1A FAPs are in the lower left. UMAPs demonstrating COL1A1, COL1A2, and COL15A1 expression across the FAP cluster and its subclusters. COL15A1 is largely absent in the CDKN1A FAP subcluster. Analysis of the cellcell communication across the IBM and NDC datasets using the CellChat package. The number of the inferred ligand/receptor interactions is indicated by the size of an arrow connecting two cell populations. The larger the arrow, the higher the number of interactions between the cell populations. The ligand/receptor pairings were tested against the CellChat library for significance. Representative flow cytometry scatter plots displaying SSC vs -bungarotoxin for primary human muscle cells (PHMC) treated with COL15A1 or COL1A1. Percentage of -bungarotoxin+PHMC treated with COL15A1 or COL1A1 or vehicle. =8 per group. Representative live/dead staining for PHMCs incubated with COL15A1 or COL1A1 in addition to 10ng/ml interferon- (INF-). Live cells were identified by green-fluorescent calcein-AM, indicating intracellular esterase activity. Dead cells were identified by red-fluorescent ethidium homodimer-1, indicating a loss of membrane integrity. 100 cells were counted for each sample, and the frequencies of live or dead cells were recorded. =8 per group. Differences between groups were analysed by KruskalWallis test followed by post hoc testing. * <0.05, *** <0.001. cyclin dependent kinase inhibitor 1A; collagen; fibro-adipogenic progenitor; non-diseased control; inclusion body myositis; immune-mediated necrotizing myopathy; uniform manifold approximation and projection",yes
PMC6658642,Figure_1,oa_package/78/cd/PMC6658642.tar.gz,"[' 1.', '*Reported in the original study as a combined finding of glomerulonephritis or tubulointersticial nephropathyKidney tissue sample from a patient with sepsis (light microscopy, HE, 20).', 'b Edema and glomerular and tubular congestion is seenCentral nervous system histopathology in sepsisSepsis-induced encephalopathy is prevalent in critically ill patients and is associated with increased mortality, and mechanisms involved in its pathogenesis are unknown [34 38].']","Fig. 1 Kidney tissue sample from a patient with sepsis (light microscopy, HE, 20). Glomerular collapse is observed and signs of acute tubular necrosis. Edema and glomerular and tubular congestion is seen",yes
PMC3052615,Figure_7,oa_package/88/76/PMC3052615.tar.gz,['36-year-old woman with palpable mass in her right breast.'],"Fig. 7 36-year-old woman with palpable mass in her right breast. On sonography (left: transverse view, right: longitudinal view), round microlobulated hypoechoic mass with microcalcifications and nonparallel orientation (arrowheads) is seen and classified as BI-RADS category 4c. Results of sonography-guided 14-gauge core needle biopsy indicate presence of stromal sclerosis, which is considered as discordant benign. Final diagnosis after surgical excision was invasive ductal carcinoma. Photomicrograph of microscopic specimen after surgical excision shows carcinoma within sclerotic stroma (Hematoxylin & Eosin staining; original magnification, 40).",yes
PMC11260939,Figure_2,oa_package/7b/a5/PMC11260939.tar.gz,"['Areas of myxoid and fibrotic background were also present ().', 'Glomus Tumor: High power microscopy revealed pathology consistent with typical glomus tumor.', 'The patient most recently completed a 21-month postoperative follow up visit.']",Fig. 2 Glomus Tumor: High power microscopy revealed pathology consistent with typical glomus tumor.,yes
PMC6202856,Figure_4,oa_package/2a/28/PMC6202856.tar.gz,"['2018-00726f3""/>Measurements in pituitary tissueGR transcripts were identified in both the pars distalis and the pars intermedia of the equine pituitary, and there was no difference in expression between healthy horses and those with PPID (A); this was confirmed by immunohistochemistry (', '11 -HSD1 expression was not different between the groups (B).', '.']","Figure 4. mRNA transcript levels of GR and glucocorticoid metabolizing enzymes in the pars distalis and pars intermedia of healthy horses and horses with PPID. mRNA transcript levels of (A) GR and (B) 11 -HSD1 were expressed in the pars distalis and pars intermedia, and levels did not differ between healthy horses and horses with PPID. (CF) Photomicrographs of the pars distalis and pars intermedia of a (C, E) healthy horse and a (D, F) horse with PPID stained for GR (brown staining).",yes
PMC3303905,Figure_6,oa_package/ba/7f/PMC3303905.tar.gz,"['However, occasionally, intravenous contrast agent administration may be required during the procedure to define the vascular structures in the anticipated needle path ().', 'Utility of intravenous contrast in delineation of vascular structures.']",Fig. 6 Utility of intravenous contrast in delineation of vascular structures. Contrast-enhanced CT of 50-year-old man shows anterior mediastinal mass and surrounding vascular structure including superior vena cava (arrowhead) and internal thoracic artery (arrow). Biopsy was obtained from right parasternal approach avoiding these vascular structures.,yes
PMC9112361,Figure_2,oa_package/d2/28/PMC9112361.tar.gz,['.'],"Figure 2. Initial CMR obtained 3 months after COVID-19. The left-side column shows the steady-state free precession (SSFP) images, while the right column shows the corresponding phase-sensitive inversion recovery (PSIR). respectively shows the 4-chamber, 2-chamber, 3-chamber, and short-axis views. The blue arrows point to areas of sub-epicardial scarring and yellow arrows show pericardial enhancement.",yes
PMC6718453,Figure_11,oa_package/48/35/PMC6718453.tar.gz,[],"Figure 11 Biochemical assay of SH-SY5Y cells treated with A, Donepezil + A, curcumin + A (CuR + A), piperine + A (Pip + A), and combined curcumin and piperine + A [(CuR + Pip) + A]. Lipid peroxidation assay, glutathione assay, catalase assay. Values were presented as mean SD, from three independent experiments. * < 0.01, compared to untreated group. < 0.05, < 0.01, compared to the A group (one-way ANOVA followed by Tukeys test). MDA, malondialdehyde; GSH, glutathione; Cat, catalase.",yes
PMC7653710,Figure_1,oa_package/75/71/PMC7653710.tar.gz,"[' 1i).', 'TWD leads to T2D, memory impairment and increased number of dystrophic neurites in mice with AD-linked genetic backgrounds.', 'AwTw = wild-type, AwT+ = Tau P301L, A + Tw = APPswe/PS1dE9, A + T+ = APPswe/PS1dE9 x Tau P301LConfocal microscopy and image analysisDetails of microglia around -amyloid plaques were analyzed from fluorescent images of the molecular layer of dentate gyrus obtained with a Zeiss Axio Observer inverted microscope equipped with a Zeiss LSM 700 or Zeiss LSM 800 confocal module (Carl Zeiss Microimaging GmbH, Jena, Germany) using 63 (NA 1.', '1a).', '1b) and 30 min glucose levels robustly elevated (F1,38 = 13.', '1c) in TWD groups as compared to STD groups.', '1d).', '1e).', '1f).', '1g).', '1h), but overall, the diet main effect was not significant (p = 0.', '1i), the brain sites with the earliest pathological changes in human AD and substantial -amyloid plaque pathology in the APPswe/PS1dE9 mouse model.', '1j and k).', '1j) or in HC (', '1k, T: p = 0.', '1l).', '1m and n).', '1l-m).', '1l-n).']","Fig. 1 TWD leads to T2D, memory impairment and increased number of dystrophic neurites in mice with AD-linked genetic backgrounds. Weight of mice at age of 12months (Two-way ANOVA). Glucose tolerance test (GTT) results after 3h fasting (Two-way ANOVA). Serum glucose levels in GTT after 30min of D-glucose injection ( =0.001, Two-way ANOVA). Representative liver sample image of TWD. Mice with TWD had significantly higher fatty liver score as compared to mice with STD ( <0.001, Mann-Whitney). Black arrows indicate lipid vacuoles. Scale bar 20m. Assessment of spontaneous activity ( <0.001, Two-way ANOVA). Passive avoidance test revealed impaired performance of mice with TWD as compared to mice with STD (diet effect, =0.02, Two-way ANOVA). Also, AD and tauopathy-associated transgenes impaired the performance (A+=APPswe/PS1dE9; <0.001 and T+=Tau P301L; <0.001, Two-way ANOVA). Three outliers were left out from statistical analysis (in brackets). In five-day learning phase of Morris swim navigation test, mice with A+ transgene (APPswe/PS1dE9) (red lines) did not learn to find the platform as fast as mice without A+ transgene (blue lines, =0.002, Two-way ANOVA), while TWD and T+ transgene (Tau P301L) did not affect learning. On day five, mice with A+ transgene spent significantly less time in platform zone ( =0.001, Two-way ANOVA). Also, mice with TWD spent less time in platform zone as compared to mice with STD, but the difference did not reach statistical significance ( <0.09, Two-way ANOVA). A representative image of APPswe/PS1dE9 mouse coronal brain section used for assessing -amyloid plaque load from lateral entorhinal cortex (LEC) and from dentate gyrus (DG) of hippocampus (HC). Amyloid burden quantification in LEC, and in HC. A representative fluorescence microscope image of APPswe/PS1dE9 mouse coronal brain section showing AT8-positive dystrophic neurites around -amyloid plaques. Scale bar=10m. Quantification of dystrophic neurites/plaque in LEC showing significant increase in TWD mice as compared to STD mice ( <0.001). Also, in HC number of dystrophic neurites per plaque was increased in TWD mice as compared to STD mice ( =0.009). All results are shown as mean+SEM, behavioral tests: =57 mice/group, Two-way ANOVA, immunohistochemistry: =56 mice/group, 3 brain slices/mouse. Dystrophic neurites around -amyloid plaques (diameter>10M) were counted, Kruskal-Wallis H-test. AwTw=wild-type, AwT+=Tau P301L, A+Tw=APPswe/PS1dE9, A+T+=APPswe/PS1dE9 x Tau P301L",yes
PMC8560595,Figure_4,oa_package/8b/64/PMC8560595.tar.gz,[],"FIGURE 4 Stromal cell development progress based on molecular trajectory analysis. (A, B) Pseudotime ordering of DS1, DS2 and DS3 in normal and RSA samples by monocle analysis. Each dot represents one cell and each branch represents one cell state. A was labelled with cell states and B was labelled with developmental pseudotime. (C) Heatmap showing the significant enriched gene dynamics along pseudotime; (DG) Violin plot showing the expression profile of , , and among normal DS subset; (H) Heatmap showing the decidualization related gene dynamics among different cell fates by branch analysis; (I, J) The expression of and along pseudotime among DS subsets in the normal and RSA group",yes
PMC6701338,Figure_1,oa_package/29/6d/PMC6701338.tar.gz,"['A triple-phase contrast-enhanced CT abdomen depicted a 14 14 cm well-defined left liver lobe mass, involving segments II, III, and VI-A and VI-B, which was hypodense in the early arterial phase, retaining the contrast in the venous phase, and showing a mixed-enhanced pattern in the delayed phase (s 1(a) 1(c)).', 'CO;2-B11596015Contrast-enhanced CT abdomen.']",Figure 1 Contrast-enhanced CT abdomen. (a) Early arterial phase showing a hypodense lesion in the left liver lobe and a normal spleen. (b) Venous phase showing a hypodense lesion confined to the left liver lobe and firmly attached to the middle hepatic vein. (c) A mixed-enhanced pattern of the lesion on the delayed phase.,yes
PMC7448496,Figure_2,oa_package/48/f9/PMC7448496.tar.gz,"[' 2a).', ' 2b).', ' 2c (Pearson r = 0.', ' 2d).', ' 2e).', ' 2f).', ' 2g).', ' 2h) and U251MG cells (', ' 2i).', ' 2j).', '\nLBX2-AS1 expression was associated with miR-491-5p levels in glioma.', '001A mutual regulatory association between miR-491-5p and LBX2-AS1 in gliomaTo study the association between miR-491-5p and LBX2-AS1, we transfected increasing concentrations (50 nM and 100 nM) of miR-491-5p mimic into U87MG and U251MG cells.']","Fig. 2 LBX2-AS1 expression was associated with miR-491-5p levels in glioma. RT-PCR detection of LBX2-AS1 expression in a panel of glioma cell lines U87MG, U251MG, A172 and normal human astrocyte (NHA). Flow chart of screen for target miRNAs of LBX2-AS1 in glioma. The association between LBX2-AS1 and miR-491-5p expression in TCGA-LGG project was analyzed by Pearson correlation analysis. RT-PCR detection of miR-491-5p expression in normal brains and gliomas. Comparison of miR-491-5p expression in normal brain, grade II glioma, grade III glioma and grade IV glioma. The association between LBX2-AS1 and miR-491-5p expression in collected glioma samples was analyzed by Pearson correlation analysis. The expression of miR-491-5p was detected in U87MG and U251MG cells transfected with miR-NC or miR-491-5p mimic. In U87MG ( ) and U251MG ( ) cells, the luciferase activity of pmirGLO (empty vector as the control group) and pmirGLO-LBX2-AS1 was detected. J. RIP assay was performed with IgG and Ago2 antibody, followed by RT-PCR detection of LBX2-AS1 and miR-491-5p levels. *, p<0.05; ***, p<0.001",yes
PMC6350530,Figure_6,oa_package/f7/7a/PMC6350530.tar.gz,"['Unexpectedly, inhibition of apoptosis had no effect on LOX, an ECM cross-linker, in the origin, midsubstance or insertion ().', '.']",Fig. 6. Inhibiting apoptosis caused no changes in LOX. ( ) Inhibition of apoptosis in fatigue-damaged tendons had no effect on LOX. ( ) Representative insertion images of LOX for 5 d of injection at day 7 and day 14. Arrows show positive staining.,yes
PMC4682951,Figure_7,oa_package/72/68/PMC4682951.tar.gz,"['g007Analysis of T cell proliferation and cytokine generation induced by pDCs and Gr-1int cells sorted from the lungs of Mtb-infected mice.', 'g007""/>Next, pDCs sorted from Mtb-infected mice at 28 days post-infection directly induced the proliferation of CD4+ and CD8+ T cells (white bars in B) and the production of IFN- and IL-2 in CD8+ T cells (white bars in E), but there were no significant changes in IFN- , IL-2, IL-5 and IL-17A secretion from CD4+ T cells (white bars in D).', 'Although pDCs sorted from Mtb H37Ra-infected mice induced Th1 (IFN- ) and Th17 (IL-17A) cytokines in T cells in the presence of BMDCs, pDCs sorted from Mtb H37Rv- and K-infected mice induced significantly greater levels of Th1 (IFN- ) and Th2 (IL-5) cytokines compared to the BMDC + CD4+ T cell groups (yellow bars in D).', 'Thus, we confirmed that CD11c-CD11b+Gr-1int cells induced by Mtb infection can suppress the T cell response (C, 7F and 7G).', 'As a result, CD11c-CD11b+Gr-1int cells sorted from Mtb-infected mice blocked the proliferation of and cytokine production by CD4+ and CD8+ T cells in the presence of BMDCs (yellow bars in C, 7F and 7G), but there was no difference in the response of T cells to CD11c-CD11b+Gr-1int cells in the absence of BMDCs (white bars in C, 7F and 7G).', 'Surprisingly, CD11c-CD11b+Gr-1int cells sorted from Mtb H37Rv- and K-infected mice inhibited IL-17A production by T cells in the presence of BMDCs compared to the BMDC + CD4+ T cell groups (yellow bars in F).', 'Interestingly, the isolated pDCs from the lungs of mice infected with virulent Mtb strains significantly induced the simultaneous production of IFN- and IL-5 in the DC-CD4+ T cell co-culture system, whereas the pDCs from Mtb H37Ra-infected mice induced IFN- and IL-17A under the same culture conditions (B, 7D and 7E).', 'Consequently, the Gr1intCD11b+ cells that was isolated from the lungs of Mtb-infected mice at 28 days post-infection significantly decreased CD4+ and CD8+ T cell proliferation under DC-T cell co-culture conditions (C).', 'In addition, IFN- , IL-2 and IL-17A levels were significantly decreased in the presence of Gr1intCD11b+ cells generated by virulent Mtb infection (F).']",10.1371/journal.pone.0145234.g007,yes
PMC6157395,Figure_2,oa_package/92/47/PMC6157395.tar.gz,"['Immunohistochemical analysis of MPO, HNE and IL-8 in kidney sections from patients with acute PUUV-HFRS.']","Figure 2 Immunohistochemical analysis of MPO, HNE and IL-8 in kidney sections from patients with acute PUUV-HFRS. Kidney sections from acute PUUV-HFRS or unrelated kidney diseases ( = 5 for both) were analyzed for the presence of MPO, HNE or IL-8 by standard immunohistochemical techniques and visualized by DAB staining. Images from three cases with acute PUUV-HFRS and one PUUV-negative control section is shown. The tissue area positive for MPO, HNE or IL-8 was evaluated as percentages in all sections and mean values standard deviation reported for acute PUUV-HFRS and PUUV-negative sections. Significant differences were assessed by student's -test and statistically significant differences indicated as < 0.05.",yes
PMC11625831,Figure_1,oa_package/2a/87/PMC11625831.tar.gz,"['However, CT on postoperative day 26 revealed anastomotic dehiscence with an associated abscess (A).', 'Tubography revealed persistent leakage (B).', 'Gel foam sheath (Cutanplast, Mascia Brunelli, Milan, Italy), meticulously tailored to the required size (E), was then introduced into the anastomosis site through the catheter assisted by a guidewire and saline.', 'Following gel foam insertion, a mixture of NBCA and lipiodol (Guerbet, Villepinte, France) at a 1:2 ratio was injected (D) continuously from the lumen of the esophagus to the retroperitoneal space after navigating the guidewire and catheter above the anastomosis site (', 'Follow-up CT scans at 6- and 32-months post-procedure showed no fluid collection at the EJ anastomosis site, indicating the absence of recurrence (F).', 'Funding: None1\nScottHWJr\nWeidnerMG\nTotal gastrectomy with Roux-en-Y esophagojejunostomy in treatment of gastric cancerAnn Surg195614368269613314542\n2\nMakuuchiR\nIrinoT\nTanizawaY\nBandoE\nKawamuraT\nTerashimaM\nEsophagojejunal anastomotic leakage following gastrectomy for gastric cancerSurg Today20194918719630317492\n3\nSierzegaM\nKolodziejczykP\nKuligJ\nPolish Gastric Cancer Study GroupImpact of anastomotic leakage on long-term survival after total gastrectomy for carcinoma of the stomachBr J Surg2010971035104220632269\n4\nAurelloP\nMagistriP\nD AngeloF\nValabregaS\nSirimarcoD\nTiernoSM\n\nTreatment of esophagojejunal anastomosis leakage: a systematic review from the last two decadesAm Surg20158145045325975326\n5\nLangH\nPisoP\nStukenborgC\nRaabR\nJahneJ\nManagement and results of proximal anastomotic leaks in a series of 1114 total gastrectomies for gastric carcinomaEur J Surg Oncol20002616817110744938\n6\nFeithM\nGillenS\nSchusterT\nTheisenJ\nFriessH\nGertlerR\nHealing occurs in most patients that receive endoscopic stents for anastomotic leakage; dislocation remains a problemClin Gastroenterol Hepatol2011920221021172455\n7\nKimJH\nShinJH\nOhJS\nRole of interventional radiology in the management of postoperative gastrointestinal leakageInt J Gastrointest Interv2022111681738\nLisleDA\nHunterJC\nPollardCW\nBorrowdaleRC\nPercutaneous gelfoam embolization of chronic enterocutaneous fistulas: report of three casesDis Colon Rectum20075025125617180253\n9\nCambj SapunarL\nSekovskiB\nMati D\nTripkovi A\nGrandi L\nDru ijani N\nPercutaneous embolization of persistent low-output enterocutaneous fistulasEur Radiol2012221991199722544294\n10\nMauriG\nPescatoriLC\nMattiuzC\nPorettiD\nPediciniV\nMelchiorreF\n\nNon-healing post-surgical fistulae: treatment with image-guided percutaneous injection of cyanoacrylic glueRadiol Med2017122889427752970\nPercutaneous embolization procedure for esophagojejunal anastomotic leakage and pre- and post-embolization CT scans in a 59-year-old female who underwent total gastrectomy with Roux-en-Y anastomosis.']","Fig. 1 Percutaneous embolization procedure for esophagojejunal anastomotic leakage and pre- and post-embolization CT scans in a 59-year-old female who underwent total gastrectomy with Roux-en-Y anastomosis. Axial image from contrast-enhanced CT scan on postoperative day 26 shows dehiscence at the anastomosis site (arrows) and air-filled cavity (*). Tubography image on postoperative day 29 demonstrates persistent leakage at the esophagojejunal anastomosis site despite ongoing conservative treatment. Fluoroscopic image shows that the guidewire and catheter navigate above the anastomosis site. Fluoroscopic image illustrating the injection of 1:2 mixture of NBCA and lipiodol after pulling back the catheter. The presence of a visible NBCA casting (arrowheads) indicates successful embolization. Gross photo of cut gel foam, soaked in saline, folded and placed inside the catheter hub. Axial image of CT scan taken at 32 months post-embolization shows no signs of leakage recurrence, with persistent glue casting (arrow). NBCA = N-butyl-2-cyanoacrylate",yes
PMC11524578,Figure_4,oa_package/82/aa/PMC11524578.tar.gz,"['These subclusters were labeled 1_3, 1_4, and 2_6 (A and B; figure supplement 1) and represent regions of the thalamus and hypothalamus (C).', '.', ' figure supplement 1.', ' figure supplement 2.', 'Next, we assessed whether EAE-specific subclusters were physically closer to meningeal inflammation than other related subclusters.', 'However, in subclusters of cluster 2, the EAE-specific subcluster was significantly closer to C11 on average compared to other subclusters (D).', 'To explore which pathways were activated in these subclusters, we performed gene set enrichment analysis using the GO database (E G; Supplementary file 4, Supplementary file 5, Supplementary file 6).', 'Interestingly, pathways related to neuron death, cellular stress, and negative regulation of cellular processes were also enriched (E G; figure supplement 2).', 'Overall, 31 upregulated pathways were conserved between cluster 11 and subclusters 1_3, 1_4, 2_6 (H).', 'We found prominent conserved upregulation of pathways related to adaptive/B-cell-mediated immunity, antigen processing and presentation, cell killing, and others (I).']","Figure 2. PROGENy analysis reveals spatially restricted pathway activity differences between nave and experimental autoimmune encephalomyelitis (EAE). (A) Heatmap displaying averaged PROGENy pathway analysis results. (B) Representative spatial plot showing activity of the JAK-STAT, NFkB, and TNFa signaling pathways. (C) Comparison of JAK-STAT, NFkB, and TNFa pathway activities between groups. (D) Representative spatial plot showing activity of the Trail, PI3K, and TGFb signaling pathways. (E) Comparison of Trail, PI3K, and TGFb pathway activities between groups. (Nave mouse N=4, sample N=5; EAE mouse N=4, sample N=6; multiple t-tests corrected for multiple comparisons with the Benjamini, Krieger, and Yekutieli method; *p<0.05, **p<0.01, ***p<0.001.)",yes
PMC11387477,Figure_2,oa_package/ee/5e/PMC11387477.tar.gz,"[' 2a; AAV.', 'Adult-onset human APP expression reduces LEC PV interneuron excitability.', 'In LEC PV interneurons, we observed a dramatic reduction in PV interneuron firing (', ' 2b, c) potentially related to a reduction in input resistance (', ' 2d), as no other relevant factors (e.', ' 2d; Supplementary ', ' 2g) despite an increase in the AP AHP (Supplementary ', ' 2h; Supplementary ', ' 2i, j; Supplementary ', ' 2) were absent following 2-3 weeks of viral mAPP expression (']","Fig. 2 Adult-onset human APP expression reduces LEC PV interneuron excitability. Graphical summary of AAV.E2.tdTom and AAV.EF1a.hAPP (or for Ctrl, saline) stereotactic injection in the Lateral Entorhinal Cortex. PV-interneurons were fluorescently targeted (tdTom+) for whole-cell current clamp recordings. AP firing elicited by square pulse current injections of varying magnitude normalized to cellular capacitance during recording in tdTom+ PV-INT from L2 LEC at 12 pA/pF. Group data summary of AP firing frequency in L2 LEC from Ctrl (black) and hAPP injected mice (magenta). LEC PV interneurons from hAPP injected mice show a significant reduction in AP Frequency (Hz) when compared to Ctrl(Ctrl: Max: 156.613.52Hz, hAPP: Max: 91.848.74Hz, <0.0001, Two-Way ANOVA). Summary data of AP properties. L2 LEC PV interneurons after hAPP injection display a significantly decreased input resistance (Ctrl: 145.711.61M, minimum: 82.33, 25% percentile: 125.8, median: 148.4, 75% percentile: 175.3, maximum: 192.9, range: 110.6; hAPP: 88.7815.11M, minimum: 43.02, 25% percentile: 56.95, median: 80.93, 75% percentile: 139.1, maximum: 189.9, range: 146.8; =0.01, =2.73, df=21) and an insignificant increase in membrane capacitance (Ctrl: 68.835.34pF, minimum: 40.49, 25% percentile: 60.14, median: 75.62, 75% percentile: 86.40, maximum: 99.78, range 59.26, hAPP: 90.219.77pF, minimum: 39.90, 25% percentile: 66.88, median: 90.51, 75% percentile: 115.2, maximum: 152.4, range: 112.5; =0.07, =1.92, df=21). . Graphical summary of AAV.E2.tdTom and AAV.EF1a.hAPP (or for Ctrl, saline) stereotactic injection in the Somatosensory Cortex. PV-interneurons were fluorescently targeted (tdTom+) for whole-cell current clamp recordings. AP firing elicited by square pulse current injections of varying magnitude normalized to cellular capacitance during recording in tdTom+ PV-INT from L5 SS Ctx at 12 pA/pF. Group data summary of AP firing frequency in L5 SS Ctx from Ctrl (black) and hAPP injected mice (magenta). SS Ctx PV interneurons from hAPP injected mice show no significant change in AP Frequency (Hz) when compared to Ctrl (Ctrl: Max: 301.127.59Hz, hAPP: Max: 257.224.06Hz). Summary data of AP properties. SS Ctx interneurons after hAPP injection display an unchanged Membrane Capacitance (Ctrl: 71.919.514, minimum: 34.82, 25% percentile: 46.09, median: 67.31, 75% percentile: 104.9, maximum: 124.2, range 89.40; hAPP: 73.147.327, minimum: 44.84, 25% percentile: 50.37, median: 68.69, 75% percentile: 98.70, maximum: 117.7, range: 72.83; =0.9180, t=0.1041, df=23) and input resistance (Ctrl: 121.217.14, minimum: 65.09, 25% percentile: 68.17, median: 112.1, 75% percentile: 137.5, maximum: 240.6, range: 175.5; hAPP: 109.110.56, minimum: 64.34, 25% percentile: 80.07, median: 89.76, 75% percentile: 148.6, maximum: 177.9, range: 113.6; =0.5475, t=0.6106, df=23). RNAscope representative images at 40x magnification for Ctrl injected (top) and hAPP injected mice (bottom): mAPP mRNA (cyan), Parvalbumin mRNA (gold), human APP mRNA (magenta), and a final merged image. RNAscope quantification for hAPP copies per PV+ cell comparing control to hAPP injected. hAPP injected show a significant increase in hAPP copies per PV+ cell ( =0.0039, =5.987, df=4; two-tailed paired t-test). For all summary graphs, data are expressed as mean (SEM). For , , and : Statistical significance is denoted as * <0.05, as determined by Two-way ANOVA with Sidaks multiple comparison test. For d, h: Individual data points (technical replicates, LEC: Ctrl =10, hAPP =13; SS: Ctrl =12, hAPP =13; all data collected from 3 biological replicates each) and box plots are displayed. Statistical significance is denoted as * <0.05, as determined by two-tailed unpaired -test. Source data are provided as a Source Data File.",yes
PMC5155155,Figure_4,oa_package/4f/05/PMC5155155.tar.gz,"['gp42 C-domain interactions with gHThe gp42 C-terminal domain (86 221) adopts a CTLD fold that binds HLA class II receptor, as well as forming interactions with gH (a).', 'Negative stain single-particle EM studies of the gHgL/gp42/HLA entry triggering complex22 indicated that the gp42 CTLD could form direct interactions with gH through a HP that had previously been postulated to consist of residues gp42:I159, gp42:V184, gp42:Y185, gp42:I187, gp42:F188, gp42:Y194, gp42:F198, gp42:V201, gp42:F210 and gp42:L211 (a,b).', 'The observed gp42 CTLD interface with gH is formed by only one edge of this predicted gp42 HP, making direct contacts with gH through 3 of these 10 HP residues (gp42:I187, gp42:F188 and gp42:F210; a,b).', 'Residues gp42:I159, gp42:Y185, gp42:V184, gp42:V201, gp42:F198, gp42:Y194 and gp42:L211 do not form contacts with gH (b).', 'The gp42 HP makes contacts with four loops within gH D-II, between helices 2 -4 and 2 -5, helices 2 -6 and 2 -7, strands 2 -4 and 2 -5 and strands 2 -6 and 2 -7 (a,b).', 'The gp42:F210 side chain, which has been shown by mutagenesis to be important for membrane fusion30, inserts into the centre of the loop between helices 2 -6 and 2 -7, against the cavity formed by an unusual central loop proline (gH:P281, a,b).', 'Residues at the junction of the gp42 N- and C-terminal domains interact with the 188KGD190 motif in gH D-II (exposed loop between 2 -6 and 2 -7), which is the putative integrin receptor-binding site required for epithelial-cell entry (c).', ""Residue gp42:W81 is 10 away from the KGD' motif (c), suggesting that these gp42-derived peptides do not inhibit membrane fusion by blocking integrin receptor binding."", 'We note that one edge of the gp42 HP engages gH, with two gp42 histidines (gp42:H205 and gp42:H206) that are poised to interact with gH:E282 (b).', 'Schematics of the proteins are shown in Supplementary .', 'org/1999/xlink"" xlink:href=""ncomms13557-f3""/>The gp42 HP interacts with gH D-II and overlaps the integrin binding KGD\' motif.']","Figure 4 The gp42 HP interacts with gH D-II and overlaps the integrin binding KGD' motif. ( ) One edge of the previously identified gp42 HP interacts with gH D-II. The gp42 C-domain surface is coloured hotpink, with HP residues shown in slate (shade of blue) and labelled. The gH D-II is shown as a cartoon and coloured wheat. Gp42 residues gp42:I187, gp42:F188 and gp42:F210 interact with gH D-II. ( ) Two gp42 histidines (gp42:H205 and gp42:H206, cyan sticks) in the HP form contacts with gH:E282 (orange). HP residues not directly in contact with gH are indicated by their C atom shown as spheres. ( ) gp42:L85 (sticks) contacts gH:G189 in the KGD motif, while gp42:E171 (cyan) is positioned to form chargecharge interactions with gH:K188. The shortest distance (10.4) between the minimal inhibitory peptide of gp42 peptide ending at gp42:W81 (ref. ) with the gH KGD motif is highlighted. Structures were rendered using MacPyMol.",yes
PMC3844243,Figure_5,oa_package/64/ba/PMC3844243.tar.gz,"['In one patient who was imaged during an acute pyelonephritis and after successful antibiotic therapy, the pathologic signal in the DWI images has completely vanished after therapy ().', '004""/>Pyelonephritis in a 31-year-old female patient of the left kidney who was imaged initially to assess the extent of renal involvement for possible operation and reimaged after 7 weeks of antibiotic therapy.']","Figure 5 Pyelonephritis in a 31-year-old female patient of the left kidney who was imaged initially to assess the extent of renal involvement for possible operation and reimaged after 7 weeks of antibiotic therapy. Left column: a clear infectious focus can be easily seen on the DWI images and on the venous T1w images and the signal alterations of the kidney appear diffuse on T2w imaging. Right column: after successful antibiotic therapy with normal CRP value, no pathologic signal changes can be seen on the DWI images. The T1w and T2w images show moderate scarring of the formerly affected parenchyma (arrows).",yes
PMC4939893,Figure_5,oa_package/ca/d9/PMC4939893.tar.gz,['Association of overall survival with the total number of mutated genes (non silent somatic mutation) in breast cancer.'],"Figure 5 Association of overall survival with the total number of mutated genes (nonsilent somatic mutation) in breast cancer. Hazard ratios (haz. rat.) are shown for a dichotomized version of the number of mutated genes that was varied along the axis. (A) In univariate analysis, no cutoff point between 10 and 180 yielded a significant association with survival. (B) In multivariate analysis including correction for patient age, tumour size, nodal status, histopathological type, tumour grade, hormone receptor status and HER2 status, a high number of mutations were borderlinesignificant associated with shorter survival for cutoff points between 18.5 and 27.5. The most significant association was obtained for a cutoff point of 21.5 with hazard ratio=4.6 (1.010.0) and =0.044.",yes
PMC5785689,Figure_6,oa_package/b8/18/PMC5785689.tar.gz,"[', 2014) thin-layer Matrigel model to culture isogenic control neurons together with either isogenic control or PSEN1 E9 mutant astrocytes ( 6A).', 'Application of glutamate, together with the co-agonist glycine, resulted in significantly lower Ca2+-transient amplitudes in healthy control neurons co-cultured with AD astrocytes when compared with the same neurons co-cultured with control astrocytes (s 6B 6D).', 'Likewise, the presence of AD astrocytes also significantly reduced the neuronal Ca2+ transients evoked by -aminobutyric acid (GABA) (s 6B, 6C, and 6E).', ' 6AD Astrocytes Influence the Calcium Signaling Activity of Healthy Neurons(A) Representative immunocytochemistry image of the thin-layer Matrigel co-culture with MAP2-positive neurons (green) and GFAP-positive astrocytes (red).']","Figure6 AD Astrocytes Influence the Calcium Signaling Activity of Healthy Neurons (A) Representative immunocytochemistry image of the thin-layer Matrigel co-culture with MAP2-positive neurons (green) and GFAP-positive astrocytes (red). Nuclei are stained with Hoechst. Scale bar, 50m. (B) Representative electrogram of isogenic control neurons co-cultured with isogenic control astrocytes showing Ca amplitudes in response to applications of glutamate and glycine, GABA, KCl, and ionomycin. (C) Representative electrogram of isogenic control neurons co-cultured with AD astrocytes showing Ca amplitudes in response to applications of glutamate and glycine, GABA, KCl, and ionomycin. (D) Quantification of the Ca amplitudes in response to glutamate and glycine application. The x axis shows the genotype of the astrocytes (isogenic CTRL, n= 35 cells; AD, n= 134 cells from 3 independent experiments with 2 isogenic pairs; p< 0.01). (E) Quantification of the Ca amplitudes in response to GABA application. The x axis shows the genotype of the astrocytes (isogenic CTRL, n= 27 cells; AD, n= 132 cellsfrom 3 independent experiments with2isogenic pairs; p< 0.001). Data are presented as mean SEM. See also .",yes
PMC3789215,Figure_4,oa_package/c4/6a/PMC3789215.tar.gz,"['To determine whether chronic SCI induces processing of caspase-1 and the pro-inflammatory cytokines IL-1 and IL-18, we analyzed VAT and pancreatic lysate, comparing chronic SCI, na ve and sham-operated control ().', 'In VAT, chronic SCI resulted in a significant increase in the proteolytic cleavage products of caspase-1 (20, 26 kDa), IL-1 (17 kDa) and IL-18 (18 kDa) when compared to na ve and sham-operated control (A).', 'Correspondingly, in pancreatic lysate chronic SCI also induced a significant increase in the proteolytic cleavage products of caspase-1, IL-1 , and IL-18 (B).', 'Immunoblot analysis of proteolytic processing and activation of caspase-1, IL-1 , and IL-18 in VAT and pancreas in control and SCI mice(A) In VAT there is a significant increase in proteolytic cleavage of pro-caspase 1 (45, 50 kDa) to enzymatically active forms (20, 26 kDa) 1-month post-SCI compared to the na ve (N) and sham-operated (S) control.']","Figure 4 Immunoblot analysis of proteolytic processing and activation of caspase-1, IL-1, and IL-18in VAT and pancreas in control and SCI mice ( ) In VAT there is a significant increase in proteolytic cleavage of pro-caspase 1 (45, 50kDa) to enzymatically active forms (20, 26kDa) 1-month post-SCI compared to the nave (N) and sham-operated (S) control. There is also a significant increase in proteolytic cleavage of IL-1 (31kDa) and IL-18 (24kDa) to their enzymatically active forms (17, 18kDa, respectively) 1-month post-SCI when compared to the nave (N) and shame-operated (S) control. ( ) In VAT there is a significant increase in proteolytic cleavage of pro-caspase 1 (45, 50kDa) to enzymatically active forms (20, 26kDa) 1-month post-SCI compared to the nave (N) and sham-operated (S) control. There is also a significant increase in proteolytic cleavage of IL-1 (31kDa) and IL-18 (24kDa) to enzymatically active forms (17, 18kDa, respectively) 1-month post-SCI when compared to the nave (N) and shame-operated (S) control. -actin was used as a protein loading control. Statistics are according to data analysis methods described. 0.05. =8 for each group.",yes
PMC11502625,Figure_3,oa_package/ce/14/PMC11502625.tar.gz,"[' 3).', 'A 74-year-old woman with T2 stage rectal tumor.', 'The dashed line indicates the boundary between the adenocarcinoma tissue and the intrinsic muscular layer, with partial invasion of the muscular layer (h, HE 100)For the T3 stage patient, the tumor infiltrates beyond the muscularis propria into the mesorectum on T2WI.']","Fig. 3 A 74-year-old woman with T2 stage rectal tumor. The axial T2WI image ( ) reveals tumor infiltrating into the muscularis propria, characterized by focal blurring (arrow) and potential infiltrating into the mesorectal fat, which can lead to inaccurate staging. On the axial SyDIR image ( ), the intact black ticking of the muscularis propria (arrow) is observed, indicating tumor involvement without infiltrating beyond the muscularis propria. The schematic diagram of SyDIR ( ) displays a low signal boundary line between the tumor margin and the intrinsic muscular layer of the intestinal wall. The tumor border was manually delineated on the SyT2WI image ( ). The quantitative magnetic resonance (MR) values ( ) of the tumor were measured as follows: 1059ms for T1, 113ms for T2, and 78pu for PD. Pathological examination confirmed the deep invasion of the muscular layer. The dashed line indicates the boundary between the adenocarcinoma tissue and the intrinsic muscular layer, with partial invasion of the muscular layer ( , HE100)",yes
PMC5973050,Figure_2,oa_package/d7/82/PMC5973050.tar.gz,['93 666 mm3 10 mm 500 mm3 10 mm PET-CT CT 2 3 [9] Xu [10] 5 mm-10 mm 0 300 mm3-500 mm3 2 47 A-D 8.'],"2 47A-D8.7 mm7.4 mm7.6 mmE218 mm 9.8 mm8.8 mm8.4 mm Male, 47 years old, A-D: a nodule (white arrow) was incidentally detected in left upper lobe, nodules margin is lobulated, manually measured the largest diameter in X, Y and Z was 8.7 mm, 7.4 mm, 7.6 mm respectively; E:3D volumetric analysis showed nodule was non-spherical, software-generated volume of the nodule was 218 mm . Automatic measured the maximum diameter was 9.8 mm, 8.8 mm, 8.4 mm respectively. Pathology result was invasive adenocarcinoma",yes
PMC7767116,Figure_1,oa_package/e2/e7/PMC7767116.tar.gz,"['The specific protocol consisted of a Triple Phase Bone Scan comprising blood flow, blood pool and delayed bone phases ().', 'x21219429Representative examples of 3-phase bone scintigraphy assessing blood flow, blood pool and delayed bone phases in patients with clinically suspected acute (active) Charcot neuroarthropathy (CN); (a) 3-phase negative scan; (b) 3-phase positive scan.']","Figure 1 Representative examples of 3-phase bone scintigraphy assessing blood flow, blood pool and delayed bone phases in patients with clinically suspected acute (active) Charcot neuroarthropathy (CN); ( ) 3-phase negative scan; ( ) 3-phase positive scan. (LTLeft) (RTRight)",yes
PMC6834287,Figure_5,oa_package/e9/8f/PMC6834287.tar.gz,"['Ser49+3fs) (A).', 'The complete reduction of Adgrg6 expression in our clonal Adgrg6 mutant cell line (Adgrg6 KO) suggested a null allele, likely due to non-sense mediated decay of the transcript (B).', 'g002"">2O), we observed reduced expression of chondrogenic markers Sox9 and Col2a1, and mild induction of the catabolic enzyme Mmp13 in Adgrg6 KO cells, demonstrating a common function of Adgrg6 in maintaining gene expression profiles in chondrogenic lineages (B).', 'We also observed increased expression of hypertrophic marker Col10a1 in the ATDC5 KO cells (B), suggesting precocious maturation and inappropriate initiation of hypertrophy upon loss of ADGRG6 function.', 'g005"">C), while Socs1 was not detectable in either unedited wild-type or Adgrg6 KO ATDC5 cells.', 'g005Adgrg6 regulates gene expression profiles and STAT3 signaling in ATDC5 cell culture.', 'To better understand the mechanism of ADGRG6-dependent regulation of STAT3 activation, we assayed a panel of known pro-inflammatory cytokines Il1a, Il1b, Il6 and Tnf in Adgrg6 KO cells maturated for 15 days, observing increased expression of both Il1a and Il6 expression (D).', '5 months of age (E).', 'Western blot analysis demonstrated that rIL-6 effectively stimulates increased expression of pSTAT3 in both unedited wild type and Adgrg6 KO cells (F and 5G).', 'Interestingly, we revealed a low level of increased pSTAT3 expression in Adgrg6 KO cell lysates that were not stimulated by rIl-6 (F and 5G).']",10.1371/journal.pgen.1008096.g005,yes
PMC11584485,Figure_2,oa_package/1e/28/PMC11584485.tar.gz,"[' 2).', 'Position of the needle in the left foramen ovale.', 'Projections: A axial, B coronal, C sagittal, D 3D reconstructionIf the CT scan confirms the needle tip is in Meckel s cave, a 2 ml syringe is used to aspirate cerebrospinal fluid (CSF).', ' 2 and 3, contributed to the establishment of the procedure methodology and assisted with manuscript preparation.', ' 2 and 3 and curated the radiological data.']","Fig. 2 Position of the needle in the left foramen ovale. Projections: axial, coronal, sagittal, 3D reconstruction",yes
PMC8393501,Figure_3,oa_package/64/07/PMC8393501.tar.gz,"['Concerning the intensity of the nuclear/perinuclear stain, scored from 0 to 3, comparable differences were also noted between the tumor periphery and core ().', 'Representative photomicrographs showing GHS-R IHC staining in GISTs, with examples of the evaluation method previously described.']","Figure 3 Representative photomicrographs showing GHS-R IHC staining in GISTs, with examples of the evaluation method previously described. Cytoplasmic immunoreactivity was observed in most cases, while a preferential staining pattern was noted in tumor periphery vs. core (IHC, anti-GHS-R antibody, 200 magnification). ( ) Nuclear immunoreactivity in spindle GIST, score 3 (intense); ( ) Nuclear immunoreactivity in epithelioid GIST, score 2 (moderate); ( ) Nuclear immunoreactivity in spindle GIST, score 1 (weak); ( ) Absent nuclear immunoreactivity in spindle GIST (score 0).",yes
PMC10686880,Figure_2,oa_package/3c/b7/PMC10686880.tar.gz,['Leriche syndrome: color and pulse wave Doppler ultrasound of abdominal aorta showing tardus parvus waveform below the level of renal arteries.'],Fig. 2 Leriche syndrome: color and pulse wave Doppler ultrasound demonstrates turbulent flow and elevated peak systolic velocity within the narrowed segment of abdominal aorta.,yes
PMC9682473,Figure_3,oa_package/6f/d1/PMC9682473.tar.gz,['.'],"Figure 3. Anteroposterior cervical spine radiograph. The patients cervical spine is deviated to the right side of the midline, and a levoconvex cervicothoracic scoliosis is evident, extending from C4 to T6, measuring 11 (). A radiopaque ventriculoperitoneal shunt is also noted on the patients right side (arrowheads).",yes
PMC4920061,Figure_1,oa_package/52/e3/PMC4920061.tar.gz,"[', USA) ().', '0 T]Cardiovasc Intervent Radiol200932320251883676717SkjeldalSLille sFFoller sGReal time MRI guided excision and cryo treatment of osteoid osteoma in os ischii: A case reportActa Orthop Scand200071637381114539418RehnitzCSprengelSDLehnerBCT-guided radiofrequency ablation of osteoid osteoma: correlation of clinical outcome and imaging featuresDiagn Interv Radiol201319330392349183519 akarMEsenyelCZSeyranMOsteoid osteoma treated with radiofrequency ablationAdv Orthop201520158072742570552220NeumannDBerkaHDornUFollow up of thirty three computed tomography guided percutaneous radiofrequency thermoablations of osteoid osteomaInt Orthop201236811152205247921AlbisinniUBazzocchiABettelliGTreatment of osteoid osteoma of the elbow by radiofrequency thermal ablationJ Shoulder Elbow Surg201423e172433112622De PalmaLCandelariRAnticoETreatment of osteoid osteoma with CT guided percutaneous radiofrequency thermoablationOrthopedics2013365818723MorassiLGKokkinisKEvangelopoulosDSPercutaneous radiofrequency ablation of spinal osteoid osteoma under CT guidanceBr J Radiol201487201400032471232224CantwellCPO ByrneJEustaceSRadiofrequency ablation of osteoid osteoma with cooled probes and impedance control energy deliveryAm J Roentgenol20061862444825RosenthalDIHornicekFJTorrianiMOsteoid osteoma: Percutaneous treatment with radiofrequency energyRadiology2003229171751294459726VanderschuerenGMTaminiauAHObermannWROsteoid osteoma: Factors for increased risk of unsuccessful thermal coagulationRadiology2004233757621549889727SimFHDahlinCDBeaboutJWOsteoid osteoma: diagnostic problemsJ Bone Joint Surg Am19755715459111284128KjarRAPowellGJSchilchtSMPercutaneous radiofrequency ablation for osteoid osteoma: Experience with a new treatmentMed J Aust2006184563651676866329WoertlerKVestringTBoettnerFOsteoid osteoma: CT guided percutaneous radiofrequency ablation and follow up in 47 patientsJ Vasc Interv Radiol200112717221138922330CribbGLGoudeWHCoolPPercutaneous radiofrequency thermocoagulation of osteoid osteomas: factors affecting therapeutic outcomeSkeletal Radiol20053470261600746231VanderschuerenGMTaminiauAHObermannWROsteoid osteoma: Clinical results with thermocoagulationRadiology200222482861209166532LindnerNJOzakiTRoedlRPercutaneous radiofrequency ablation in osteoid osteomaJ Bone Joint Surg Br200183391961134142633LindnerNJScarboroughMCiccarelliJMCT controlled thermocoagulation of osteoid osteoma in comparison with traditional methodsZ Orthop Ihre Grenzgeb1997135522279499519Ablation of an osteoid osteoma of the left proximal metaphyseal tibia.']",Figure 1 Ablation of an osteoid osteoma of the left proximal metaphyseal tibia. ( ) CT scan demonstrates the nidus [arrow] of the osteoid osteoma. ( ) During ablation procedure the tiny RF ablation probe being inserted into the nidus through a needle. ( ) After the procedure.,yes
PMC9579814,Figure_3,oa_package/3c/28/PMC9579814.tar.gz,"['91 Extreme corneal thinning or actual perforation involving extensive areas of the cornea benefit form PK ().', '92Total penetrating keratoplasty with overlying amniotic membrane placement in a large area of corneal perforation in rheumatoid arthritis with unstable ocular surface.']",Figure 3 Total penetrating keratoplasty with overlying amniotic membrane placement in a large area of corneal perforation in rheumatoid arthritis with unstable ocular surface.,yes
PMC11565818,Figure_1,oa_package/dc/e8/PMC11565818.tar.gz,"['Counterfactual explanations answer the question: how should an image look to be classified as class X ().', 'For the latter, we focused on hepatobiliary cancers (hepatocellular carcinoma [HCC], and cholangiocarcinoma [CCA]) and lung cancers (lung adenocarcinoma [LUAD], and lung squamous cell carcinoma [LUSC]) (b).', 'We started by training a diffusion autoencoder alongside a feature extractor (c) on the NCT-CRC-HE-100k dataset, representing tissue classes in colorectal cancer (', 'We then hypothesized that a logistic regression classifier trained on the extracted features (d) could linearly separate two major classes, normal colon mucosa (NORM) and dysplastic epithelium (TUM) (', 'This classifier guided the manipulation of feature vectors, which conditioned the diffusion-based decoder (f), enabling us to generate counterfactual images for patches from the CRC-VAL-HE-7K dataset with varying degrees of manipulation.', 'Counterfactuals Enhance Interpretation of Linear Liver Cancer PredictionsWe further validated our method by training a linear MoPaDi classifier (d) to distinguish two hepatobiliary cancer types, HCC and CCA, and generating related counterfactuals.', 'DatasetsThis study explored multiple datasets (b), starting with the publicly accessible NCT-CRC-HE-100K dataset42.', 'The model consists of a conditional DDIM image decoder that is also conditioned on a feature vector obtained with a simultaneously trained feature extractor (c).', ', tissue types, we train a logistic regression classifier on 512-dimensional features extracted with a pretrained MoPaDi feature extractor (d).', 'For linearly non-separable classes, we utilized the MIL approach, which involved attention-based classifiers consisting of layer normalization followed by pooling with a multi-head cross-attention layer and a self-attention block (f).', 'The gradients for the counterfactual class serve as the manipulation directions and are added with varying amplitudes to the original feature vectors (f).', ' |Overview of the experimental setup.']","Fig. 1 | Overview of the experimental setup. Counterfactual explanations answer the question: how should an image look to be classified as class X. Datasets used in this study and preprocessing steps. The colorectal and breast cancer datasets from the TCGA, consisting of digitized WSIs, were resized to 512 512 px at 0.5 MPP, tessellated, and filtered only to include pathologist-annotated tumor regions. The NCT-CRC-HE-100k dataset included 100,000 pre-color-normalized with Macenkos method tiles across nine tissue classes. TCGA Pan-cancer dataset featured tiles from tumor regions of 32 solid cancer types. All datasets, except NCT-CRC-HE-100k, which had a separate test set CRC-VAL-HE-7K, were divided into training and hold-out test sets based on patient-level splits. The diffusion autoencoder is trained simultaneously as a feature extractor and a denoising diffusion implicit model (DDIM), which is conditioned on feature vectors and the original image encoded into noise. This approach allows the model to reconstruct either original or manipulated images. The most straightforward approach to determine how manipulations of feature vectors should be performed is to train a tile-based logistic regression classifier on feature vectors. This approach allows the manipulation of feature vectors towards the opposite class in a linear direction. For histopathological WSIs, labeling each tile as positive, e.g., for a molecular biomarker, is not entirely precise because only a few tiles might show representative features. Therefore, to address this problem, we implemented multiple instance learning (MIL) into MoPaDi, enabling predictions to be made on a patient level. Extracted feature vectors are shifted towards the opposite class by multiplication with varying amplitude and normalized class direction vector. Subsequently, a denormalized manipulated feature vector is used to condition DDIM, yielding a manipulated image. MLP, multilayer perceptron; MPP, micrometers per pixel.",yes
PMC3205758,Figure_8,oa_package/a4/f8/PMC3205758.tar.gz,"['Ruptured mucinous ovarian or appendiceal tumours may result in pseudomyxoma peritonei, which results from gelatinous tumour deposition, and may have a distinctive imaging appearance ()[50,51].', 'Pseudomyxoma peritonei.']",Figure 8 Pseudomyxoma peritonei. Axial contrast-enhanced CT shows the typical excessive scalloping of the liver and spleen from intraperitoneal mucin.,yes
PMC9293257,Figure_2,oa_package/84/ce/PMC9293257.tar.gz,"['USG: Ultrasonography(A) Contrast-enhanced axial CT image showing bowel within bowel along with mesenteric fat and vessels, (B) Contrast-enhanced axial CT showing well-defined fat attenuation lipoma (white arrow) as the leading point of ileocolic intussusception.']","Figure 2 (A) Contrast-enhanced axial CT image showing bowel within bowel along with mesenteric fat and vessels, (B) Contrast-enhanced axial CT showing well-defined fat attenuation lipoma (white arrow) as the leading point of ileocolic intussusception.",yes
PMC9847486,Figure_2,oa_package/53/20/PMC9847486.tar.gz,['Clinically diagnosed syringoma on (a) eyelid and on (b) faceThe FNAC findings are shown in Table 1.'],Figure 2 Clinically diagnosed syringoma on (a) eyelid and on (b) face,yes
PMC7556876,Figure_26,oa_package/8d/14/PMC7556876.tar.gz,[],"Figure 8figure supplement 2. Model of Full-Length and Non-extended Matriglycan Synthesis. ( ) Mature matriglycan is a long polysaccharide that is synthesized by LARGE1. ( ) In the absence of the core M3 phosphate added by POMK, LARGE1 generates a shorter, non-extended form of matriglycan.",yes
PMC3636042,Figure_5,oa_package/aa/5f/PMC3636042.tar.gz,"['D29 treatment prevents DLN destructionAnalysis of histopathology at day 68 post-infection showed that, in non-treated animals, the structure of the DLN was damaged, with absence of organized germinal centers leading to the destruction of the lymphoid tissue (A), as recently reported in experimental M.', 'On the other hand, in D29 phage-treated mice the structure of the DLN was maintained with mild alterations (B).', 'g005Histology and leukocyte kinetics in DLN of non-treated mice or mycobacteriophage D29-treated mice.', '05) at day 33 post-infection, followed by a sharp decrease observed at day 68 post-infection (C).']",10.1371/journal.pntd.0002183.g005,yes
PMC10807876,Figure_9,oa_package/10/04/PMC10807876.tar.gz,['Arthroscopic image of the repaired labrum.'],Figure 9 Arthroscopic image of the repaired labrum.,yes
PMC7405371,Figure_1,oa_package/6d/3e/PMC7405371.tar.gz,"[' 1.', '', 'a, b FOXA1 strong and diffuse immunoexpression in two bladder cancer specimens, one NMIBC (a) and one MIBC (b); c, d: GATA3 strong and diffuse immunoexpression in two bladder cancer specimens, one NMIBC (c) and one MIBC (d); e, f: CK5/6 strong multifocal immunoexpression in two bladder cancer specimens, one NMIBC (e) and one MIBC (f)Correlation between luminal/basal markers mRNA expression and protein expressionWe then checked for reproducibility between protein and transcript levels of the markers under study.']","Fig.1 Immunoexpression of luminal and basal markers in the bladder cancer cohort. , FOXA1 strong and diffuse immunoexpression in two bladder cancer specimens, one NMIBC ( ) and one MIBC ( ); , : GATA3 strong and diffuse immunoexpression in two bladder cancer specimens, one NMIBC ( ) and one MIBC ( ); , : CK5/6 strong multifocal immunoexpression in two bladder cancer specimens, one NMIBC ( ) and one MIBC ( )",yes
PMC10598367,Figure_2,oa_package/a6/5d/PMC10598367.tar.gz,"['The elevated lipid peroxidation was observed in all experimental time periods in relation to the prolonged ROT ingestion in the hippocampus of the brain compared\nto control (a) oxidative destruction of polyunsaturated fatty acids (PUFAs) of membrane phospholipids is a phenomenon generally termed lipid peroxidation.', 'Neuronal morphology alterations in pyramidal and dopaminergic neurons in hippocampus and\nSNpc observed in ROT group sections compared to the control group ( and ).']",Figure 2 Rotenone-induced histopathological alterations in the hippocampus region of the brain in absence and presence neuroprotectants.,yes
PMC10652029,Figure_1,oa_package/d8/7e/PMC10652029.tar.gz,['\nSkin lesions\n\nNumber of cases\n\nPercentage (%)\n\nNon-infectious vesiculobullous and vesiculopustular diseases\n\n8\n\n16\n\nPemphigus vulgaris\n\n5\n\n10\n\nSubepidermal bullous disease\n\n2\n\n4\n\nSpongiotic dermatitis\n\n1\n\n2\n\nNon-infectious erythematous papular and squamous lesions\n\n5\n\n10\n\nPsoriasis\n\n2\n\n4\n\nLichen planus\n\n1\n\n2\n\nPityriasis rosea\n\n1\n\n2\n\nUrticaria\n\n1\n\n2\n\nMicrobial diseases\n\n16\n\n32\n\nLeprosy\n\n12\n\n24\n\nTuberculosis\n\n2\n\n4\n\nSporotrichosis\n\n1\n\n2\n\nChromoblastomycosis\n\n1\n\n2\n\nConnective tissue disease\n\n1\n\n2\n\nDiscoid lupus erythematous\n\n1\n\n2\n\nVascular disease\n\n2\n\n4\n\nLeucocytoclastic vasculitis\n\n2\n\n4\n\nInflammatory disease\n\n1\n\n2\n\nAtopic dermatitis\n\n1\n\n2\nHematoxylin and eosin-stained photomicrograph of lepromatous leprosy showing atrophic epidermis and dermis showing histiocytes.'],Figure 1 Hematoxylin and eosin-stained photomicrograph of lepromatous leprosy showing atrophic epidermis and dermis showing histiocytes.,yes
PMC3083051,Figure_1,oa_package/ab/e5/PMC3083051.tar.gz,"['A computerized tomography (CT) scan of the abdomen revealed 2 hypervascular lesions, 30mm x 22mm in the body and 15mm x 14mm in the tail of the pancreas ().', '.']",Figure 1. Computed tomographic view of pancreatic lesions.,yes
PMC7150406,Figure_1,oa_package/22/96/PMC7150406.tar.gz,"['Following variables or measurements were taken both on the right and left sides in cranial CT scan15 ().', 'Measurements taken from the midpoint of pterion.']",Fig.1 Measurements taken from the midpoint of pterion. 1: Horizontal distance from posterolateral aspect of frontozygomatic suture. 2: Vertical distance from posterolateral aspect of frontozygomatic suture. 3: Vertical distance from zygomaticotemporal arch.,yes
PMC9420252,Figure_2,oa_package/8f/22/PMC9420252.tar.gz,"[' 2a).', ' 2b), which was confirmed by PI staining assay (', ' 2c).', ' 2b, c, d).', ' 2e), supporting the involvement of apoptosis.', ' 2f), indicating that high glucose induces both necroptosis and apoptosis.', 'Effects of RIPK3 inhibition on cell death in cardiomyocytes.', '05 versus HG + Vehicle or HG + WT (ANOVA)To verify the role of RIPK3, we used RIPK3 knockout cardiomyocytes.', ' 2g, h, high glucose increased the levels of phosphorylated MLKL in wild-type but not in RIPK3 knockout mouse cardiomyocytes, and high glucose-induced LDH release was much less in RIPK3 knockout compared with wild-type mouse cardiomyocytes.']","Fig. 2 Effects of RIPK3 inhibition on cell death in cardiomyocytes. Adult mouse cardiomyocytes isolated from RIPK3 knockout (RIPK3-KO) and wild-type mice (WT) were incubated with high glucose or mannitol (25mmol/L) in the presence of GSK872 and CHO alone or in combination for 24h. The levels of phosphorylated MLKL were determined by western blot analysis. Upper panel: a representative western blot for phosphorylated MLKL (p-MLKL), total MLKL and GAPDH; Bottom panel: quantification of p-MLKL relative to total MLKL. LDH was measured in culture medium. Necrotic cell death was assessed using PI staining. A representative micro-photograph for PI staining positive cells (red), nucleus Hoechst33342 staining (blue) and FITC-WGA staining for cell membrane (green). Quantification of necrotic cell death. and LDH was measured in culture medium. The phosphorylated levels of MLKL were determined in WT and RIPK3-KO cardiomyocytes. Upper panel: a representative western blot for p-MLKL, total MLKL and GAPDH; Bottom panel: quantification of p-MLKL relative to total MLKL. LDH was measured in culture medium. Data are meanSD, n=5 different cultures. * <0.05 versus Mannitol+Vehicle or Mannitol+WT and <0.05 versus HG+Vehicle or HG+WT (ANOVA)",yes
PMC8789645,Figure_16,oa_package/37/bb/PMC8789645.tar.gz,[],"Fig. 16 Marrow signal changes with high likelihood for osteomyelitis of the fifth metatarsal head in a 54-year-old diabetic female. Short axis T1 ( ) and T2 fat-suppressed ( ) images of the forefoot show lateral ulceration (arrows, ). Signal in the adjacent fifth metatarsal head is discordant- normal signal on T1 (arrowhead, ), with subcortical bone marrow edema-like signal on fluid sensitive images (arrowheads, ). In the presence of an adjacent soft tissue infection, findings should be considered to represent a high likelihood for early osteomyelitis",yes
PMC10734307,Figure_7,oa_package/33/ed/PMC10734307.tar.gz,"['The analysis compared CSFV-infected groups cs-1DPI (A), cs-3DPI (B), cs-5DPI (C), cs-7DPI (D) with mg2.', 'The different metabolites among the samples showed good similarity settlement relationships at all-time points after infection, which indicated that the intra-group differences in metabolic pathway changes caused by CSFV infection were small, and differences between the groups are obvious (s 7A D).', 'Heatmap of differentiated metabolic pathways between CSFV-infected and mg2 on 1DPI (A), 3DPI (B), 5DPI (C), and 7DPI (D), respectively.']","Figure 7 Heatmap of differentiated metabolic pathways between CSFV-infected and mg2 on 1DPI , 3DPI , 5DPI , and 7DPI , respectively.",yes
PMC5025881,Figure_3,oa_package/ff/e1/PMC5025881.tar.gz,"['After performing a volumetric reconstruction of the raw 2D scans, neuronal fiber bundles became visible at several levels within the tunnel of Corti and space of Nuel along the length of the imaged tissue ().', 'org/1999/xlink"" xlink:href=""srep33288-f2""/> OCT image of nerve fiber bundles traversing the tunnel of Corti and space of Nuel to innervate outer hair cells (500 m 500 m).']","Figure 3 OCT image of nerve fiber bundles traversing the tunnel of Corti and space of Nuel to innervate outer hair cells (500m500m). ( ) Volumetric reconstruction of maximum-projected OCT image stack, depicting bundles of nerve fibers traversing the organ of Corti towards the outer hair cell region. The schematic in the top right-hand corner shows the orientation of the virtual sectioning plane. Scale=150m. ( ) Schematic representation of the microanatomy in the top panel, with bundles of nerve fibers (NF) crossing the tunnel of Corti (TC) and/or the space of Nuel (SN). OPC=outer pillar cells. Scale=150m. ( ) For reference, a confocal laser scanning microscopy image of the guinea pig organ of Corti. Rhodamine phalloidin (red) marks outer and inner pillar cells (OPC and IPC, respectively), Hoechst stain (blue) marks cell nuclei, and neurofilament-H (green) marks neuronal fibers. Scale=50m.",yes
PMC5638398,Figure_3,oa_package/ac/f4/PMC5638398.tar.gz,['g00343-year-old female patient with 4.'],10.1371/journal.pone.0186242.g003,yes
PMC4932197,Figure_1,oa_package/12/c1/PMC4932197.tar.gz,"['Correlated with the presence of neuritic plaques was hyperphosphorylation of tau occurring within dystrophic neurites (a).', 'The hyperphosphorylated tau was seen surrounding the central A core but never co-localized with it (b); while closely associated, there is no direct physical interaction between the central A core and the hyperphosphorylated tau.', 'Moreover, the hyperphosphorylated tau was localized adjacent to ubiquitinated proteins within dystrophic neurites (c).', 'Because tau was not hyperphosphorylated before the formation of neuritic plaques and its biochemical alteration persisted during aging (d,e), we infer that the neuritic plaque serves as the crucible that facilitates the hyperphosphorylation of tau.', 'However, this persistent neuritic plaque-dependent biochemical alteration of tau failed to convert to tangle-like aggregates even in aged APPswe;PS1 E9 mice that were beyond 24 months as judged by Gallyas preparation (f), a silver stain method for detecting conformational changes in tau.', 'Neuritic plaques stimulate the phosphorylation of tau.']","Figure 1 Neuritic plaques stimulate the phosphorylation of tau. ( ) Brain sections of mice ( =7) were detected by antibodies specific to A (6E10), ubiquitin, Microtubule-associated protein 2 (Map2), neurofilament (Smi31, Smi312, and NF-H), and antibodies specific to phosphorylated tau: CP13 and PHF-1, Tau pT231, Tau pS262, Tau pS396, and Tau-pS422. Note the accumulation of phosphorylated tau surrounding the neuritic plaques. Scale bar, 50m. ( ) Confocal microscopic analysis of A and tau in cortex of mice ( =6). Brain sections co-stained with antiserums specific to: A (6E10) and tau (pS262) (upper panel); or A (6E10) and tau (pS422) (lower panel). Scale bar, 50m. ( ) Confocal microscopic analysis of Ubiquitin and tau in cortex of mice ( =6). Brain sections co-stained with antiserums specific to: Ubiquitin and tau (CP13) (upper panel); or Ubiquitin and PHF-1 (lower panel). Scale bar, 50m. ( ) Accumulation of phosphorylated tau in dystrophic neurites surrounding the central A core in 6-month-old mice ( =9) as detected by antibodies specific to A (6E10) and phosphorylated tau (pS422). Scale bar, 100m. ( ) Accumulation of phosphorylated tau in dystrophic neurites surrounding the central A core in 12-month-old mice ( =11) as detected by antibodies specific to A (6E10) and phosphorylated tau (pS422). Scale bar, 100m. ( ) No Gallyas positive tau tangle was detected around the A core of neuritic plaques (Congo red, arrows) in mice ( =9), even up to 24 months. Scale bar, 25m.",yes
PMC8528669,Figure_3,oa_package/6a/f4/PMC8528669.tar.gz,"['Pathologic assessment of the mass and IVC thrombus was consistent with HCC ().', ')Post-operatively, the patient was referred to oncology for HCC workup.']","Fig. 3 Histology of tumor. (a) H&E staining demonstrating tumor has a trabecular growth pattern with eosinophilic cytoplasm and prominent nucleoli. (b) Some tumor cells show clear cytoplasm. (c) Cells stained with HepPar-1 show diffuse cytoplasmic positivity in tumor cells. (d) Immunological staining for CD10 demonstrate canalicular pattern positivity. Slides were negative for inhibin, Melan A, chromogranin, synaptophysin, and GATA-3. (color photo). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)",yes
PMC8475528,Figure_12,oa_package/ab/31/PMC8475528.tar.gz,[],"FIG 12 rVSV-D1762A-S immunization prevents SARS-CoV-2 antigen expression in the lungs. Immunohistochemistry (IHC) staining of lung sections from hamsters euthanized at day 4 after SARS-CoV-2 challenge is shown. Lung sections were stained with anti-SARS-CoV-2 N antibody. The same lung sections shown in are presented to show the correlation of pathological change and SARS-CoV-2 antigen distribution. Micrographs with 1, 2, 4, and 10 magnification are shown. Scale bars are indicated at the left corner of each image.",yes
PMC4650951,Figure_10,oa_package/04/a7/PMC4650951.tar.gz,[],"Fig. 10 In stent restenosis depicted by the Ocelot OCT catheter in the course of CTO recanalization (panel ) and the corresponding angiographic image (panel ). The strait arrow annotates the stent struts, and the dashed arrow annotates the occlusive neo-intimal tissue",yes
PMC8652340,Figure_1,oa_package/c6/7e/PMC8652340.tar.gz,"['The PEIR website provides a scrollable list of the autopsy slides [].', '[10]The University of Alabama Birmingham Pathology Educational Instructional Resource (PEIR) Coronavirus Disease 2019 (COVID-19) virtual biorepository scrollable list of slides available for online viewing.']",Figure 1 The University of Alabama Birmingham Pathology Educational Instructional Resource (PEIR) Coronavirus Disease 2019 (COVID-19) virtual biorepository scrollable list of slides available for online viewing.,yes
PMC10616243,Figure_2,oa_package/46/a7/PMC10616243.tar.gz,"['In our series, we performed a bypass between the proximal and distal parts of the caudal loops within the same PICA (Case 6) ().', 'Preoperative CT scan showed a subarachnoid hemorrhage in the interpeduncular cistern (arrow) in a 66-year-old male (Case 6) (A).']","Figure 2 Preoperative CT scan showed a subarachnoid hemorrhage in the interpeduncular cistern (arrow) in a 66-year-old male (Case 6) . Right vertebral angiography revealed a fusiform aneurysm (arrow) at the lateral medullary segment (p2) of the right posterior inferior cerebellar artery (PICA) . Three-dimensional angiography confirmed the diagnosis and showed that the PICA ran in an unusual pattern, forming a loop between p1 (arrowhead) and p3 (arrow) segments . After sufficient dissection, the proximal and distal parts of the loop were easily approximated and temporally occluded by three clips . After the bypass was completed, the aneurysm was trapped . Intraoperative infrared indocyanine green angiography confirmed the patency of the anastomosis . The schematic diagram illustrates the treatment strategy for this patient . Postoperative angiography demonstrated the obliteration of the fusiform aneurysm . Three-dimensional angiography showed the patent anastomosis (arrow) .",yes
PMC6832563,Figure_2,oa_package/d4/ca/PMC6832563.tar.gz,"['Acute endophthalmitis manifests as a massive purulent reaction in the anterior and vitreous chambers, histologically represented by a massive granulocyte infiltrate, which can disarrange the fibro-muscular structure of the ciliary body, as illustrated in .', 'Ciliary body biopsy from a 90-years-old male with acute suppurative endophthalmitis.']",Figure 2 Ciliary body biopsy from a 90-years-old male with acute suppurative endophthalmitis. Hematoxylin and eosin (H&E) staining shows a massive influx of polymorphonuclear neutrophils that fragments the fibro-muscular tissue of the ciliary body and also displaces and compresses the pigmented (arrowheads) and non-pigmented (arrows) layers of the ciliary epithelium. Magnification: 400; scale bar: 30 m.,yes
PMC2813286,Figure_3,oa_package/52/dd/PMC2813286.tar.gz,"[""g003Gomori's tri-chrome stained slides of cardiac tissue showing increased fibrosis in mdx mice.""]",10.1371/journal.pone.0008976.g003,yes
PMC5976846,Figure_1,oa_package/8c/40/PMC5976846.tar.gz,"['Isolated EVs were confirmed using electron microscopy, which revealed the presence of vesicles 30 to 100 nm in size that showed the typical morphological features of exosomes as described ( 1a and b).', '28 We were able to detect these mRNAs in urine and EV, and EV-depleted samples, and interestingly found elevated levels of AQP2 and NPHS2 in patients with MA with overt nephropathy (overt) when compared with matched normoalbuminuric (non-MA) patients (normal) ( 1c) in urine and EV fractions (urine P = 0.', 'Conversely, we did not see significant concentration differences of these transcripts between normal and overt patients in their respective EV-depleted fractions ( 1c), suggesting most of these mRNAs are present in EVs.', '009604, respectively; 1d).', 'We did not see significant normal versus overt differences in the NPHS1/AQP2 or NPHS2/AQP2 ratios in EV-depleted fractions ( 1d).', ' 1Isolation of extracellular vesicles (EVs) from urine of patients with type 1 diabetes (T1D).']","Figure1 Isolation of extracellular vesicles (EVs) from urine of patients with type 1 diabetes (T1D). (a) Scanning electron micrograph of EVs isolated using size-exclusion chromatography (SEC) from urine. Bar in lower left indicates 200 nm for reference. (b) Higher-resolution image from boxed region in (a). Bar in lower left indicates 100 nm for reference. (c) Quantitative polymerase chain reaction results for mRNA kidney markers Aquaporin2 (AQP2), Nephrin (NPSH1), and Podocin (NPHS2) in urine, EV, and EV-depleted (EV-dep) samples for control (green bars, non-T1D), normal (blue bars, T1D nonmicroalbuminuria), and overt (orange bars, T1D overt diabetic nephropathy status) patient samples. Values are represented as the inverse of C values (maximum number of cycles C ) to give the linear range, which is directly proportional to the concentration of each mRNA in each sample. (d) The ratio of NPHS1 (white bars) and NPHS2 (solid bars) to AQP2 (NPHS1: AQP2, NPHS2: AQP2) from linear range values in (c) is shown for control, normal, and overt patient samples in urine, EV, and EV-dep samples. Statistically significant ( 0.05) comparisons are indicated by an asterisk.",yes
PMC3186898,Figure_4,oa_package/b0/a4/PMC3186898.tar.gz,"['Temporal changes in SERT-Te Nv and size in 3xTg-AD miceWe observed an increase in SERT-Te Nv in the CA1 subfield of the hippocampus in a strata-specific manner (s 4a and b).', '0064, respectively; a).', '0329 at 18 months; b).', '0413) although their size remain stable (s 4a and 5a).', 'org/1999/xlink"" xlink:href=""cddis201179f3""/>Bar graphs showing the age effect on SERT-Te Nv within overall CA1 subfield of the hippocampus (a) and stratum moleculare of the CA1 (b) at 3 and 18 months in non-Tg control and 3xTg-AD.']","Figure 4 Bar graphs showing the age effect on SERT-Te N within overall CA1 subfield of the hippocampus ( ) and stratum moleculare of the CA1 ( ) at 3 and 18 months in non-Tg control and 3xTg-AD. Bars represent meanS.E.M. <0.05, <0.01 compared with age-matched non-Tg control, <0.05 compared with 3 months non-Tg control. ( ) Representative electron micrographs illustrating SERT-Te density in the CA1 stratum moleculare subfield of the hippocampus at 3 and 18 months in non-Tg control ( and ) and 3xTg-AD mice ( and ). Scale bar 500nm ( ). M, mitochondria",yes
PMC4229875,Figure_17,oa_package/d2/14/PMC4229875.tar.gz,[],Figure 17 Transverse section of a cortical penetrating vessel from the frontal cortex of a rat that received three 74.5 kPa blast exposures and was sacrificed 6 months after the last exposure. Note the disruption of the tunica media at the site of the arrow and the presence of a vacuolated region (arrowhead) that extends into the adventitia. A smooth muscle (SM) cell is indicated. The surrounding neuropil appears normal. Scale bar: 10 m.,yes
PMC5960537,Figure_1,oa_package/a3/d9/PMC5960537.tar.gz,"['The preliminary transvaginal ultrasound report indicated an 8-week intrauterine pregnancy with cardiac activity, but the subsequent official report by a radiologist confirmed a cervical ectopic pregnancy (CEP) located at the posterior wall of the cervical canal ().', '1007/s00404-008-0775-4Transvaginal ultrasound showing a live ectopic pregnancy (white arrow) in the posterior wall of the cervix (black arrows).']","Figure 1 Transvaginal ultrasound showing a live ectopic pregnancy (white arrow) in the posterior wall of the cervix (black arrows). FHR 174pbm. Estimated fetal age was 8 weeks, 1 day.",yes
PMC2213052,Figure_1,oa_package/c5/b5/PMC2213052.tar.gz,"[', A and B, shows that compared with cells from WT mice, LN cells from Daf1 / mice proliferated more vigorously and secreted more IFN- .', 'Restimulation of LN cells from Daf1 / mice immunized with a different antigen, myelin oligodendrocyte glycoprotein (MOG) peptide (MOG 38 50) bearing a T cell epitope (23), produced similar results, with Daf1 / cells displaying increased proliferation and IFN- secretion compared with WT cells (, C and D).', '.', ' E shows that cultured lymphocytes from Daf1 / mice again secreted significantly increased amounts of IFN- , as noted in the previous experiment, and they also produced higher levels of IL-2 and IL-4 (', 'On the other hand, we found that the production of the inhibitory cytokine IL-10 by the Daf1 / cells was markedly reduced ( H).']","Figure 1. Responses of C57BL/6 WT and Daf1 mouse lymphocytes to antigen restimulation. (AD) LN cells from four mice in each group were pooled and restimulated with antigen in vitro (in triplicate assays) 12 d after immunization with OVA (A and B) or MOG 3850 (C and D). Cell proliferation (A and C) and IFN- production (B and D) were determined. Similar results were obtained with splenocytes (not depicted). Results are representative of three independent experiments. (EH) Spleen and LN cells from each mouse were combined and restimulated with antigen in vitro (in triplicate assays) 60 d after immunization with OVA, and the production levels of four cytokines were determined (each bar represents a single mouse, and four mice were used in each group). The x axis represents antigen concentration during restimulation assays. Asterisks designate levels that were below the detection limits. Results are representative of three independent experiments.",yes
PMC1829398,Figure_2,oa_package/ff/eb/PMC1829398.tar.gz,"['- new onset DPLD in a 57 year old female (case 2, figure 2)Case 2 new onset DPLD.']",Figure 2 Case 2 new onset DPLD. Abbreviations: BAL: bronchoalveolar lavage.,yes
PMC7084026,Figure_2,oa_package/51/a4/PMC7084026.tar.gz,"['PA type 5, originating from the accessory obturator arteryRegarding the number of independent PA, there was one PA in 95.']","Figure 2 PA type 5, originating from the accessory obturator artery",yes
PMC7530305,Figure_1,oa_package/f9/13/PMC7530305.tar.gz,"['No invasion of nearby structures was mentioned (, ).', 'CT scanner and MRI: 450 250 mm right retroperitoneal liposarcoma pushing the kidney and bowels to the other side.', '']",Fig. 1 CT scanner and MRI: 450250 mm right retroperitoneal liposarcoma pushing the kidney and bowels to the other side.,yes
PMC10045796,Figure_7,oa_package/ae/b4/PMC10045796.tar.gz,"['For further analysis, we present in and the attention maps of questions and images obtained for samples from both datasets.', 'Considering the RAD-VQA dataset samples displayed in , for the first two samples the model provides the correct answers, and the attention maps highlight the arterial region in the first image and the mesenteric arteries in the second image.', 'Attention maps obtained for sample images from RAD-VQA dataset.']",Figure 7 Attention maps obtained for sample images from RAD-VQA dataset.,yes
PMC7785558,Figure_1,oa_package/9d/cf/PMC7785558.tar.gz,"[' 1a).', ' 1b).', ' 1c and suppl.', ' 1c and suppl.', ' 1d, e and suppl.', ' 1f and suppl.', 'SH-SY5Y cells expressing APPswe display altered mitochondrial structure and function.', '0001 versus control using Mann Whitney testMitochondria are dynamic organelles requiring appropriate finely tuned equilibrium between fission and fusion.', 'a-e, online resource).', 'a, online resource), the protein levels of DRP1, MFN1, and MFN2 were significantly lower in APPswe cells in both total and mitochondria-enriched protein extracts (Suppl.', 'b-e, online resource).', ' 1g, h), while the levels of mitochondrial complexes subunits II, III, IV, and V remained unchanged (', ' 1g and suppl.', 'f, online resource).', ' 1i), but not of complexes II, III, IV, and V (Suppl.', 'g-j, online resource) in APPswe cells.', 'k, online resource).', ' 1j, k).', ' 1b.', 'a, online resource), mitochondrial size alteration observed upon -secretase inhibition is not corroborated by a modulation of DRP1 and MFN2 mRNA levels (Suppl.', ' 1a, f and suppl.']","Fig. 1 SH-SY5Y cells expressing APPswe display altered mitochondrial structure and function. Electron microscopy ultrastructure of SH-SY5Y cells stably transfected with pcDNA3.1 empty vector (control) or with APPswe cDNA (APPswe). Scale bars correspond to 2 or 1m. nucleus. Colored arrowheads indicate mitochondria. Representative images of mitochondria classes I, II, III, and IV ( ), and their quantitative distribution ( ) in control and APPswe cells. Quantitative graphs of the meansSEM of mitochondria area (m ) ( ), perimeter (m) ( ), and number/10 m ( ). Data were obtained in two independent experiments performed in duplicates. The quantification was done in at least 20 different fields (>150 mitochondria). SDS-PAGE of mitochondrial OXPHOS subunits at low and high exposures revealed in total cell extracts revealed using OXPHOS antibodies mix. Complex I-NDUFB8 subunit expression presented as meansSEM ( =4) of control (taken as 100%). Spectrophotometric analysis of the respiratory chain complex I activity expressed as absolute values in nanomols of substrate/min/mg of proteins and presented as meansSEM ( =3). Confocal images of TMRM fluorescence ( ) (scale bars represent 20m), and quantification of TMRM fluorescence intensity ( arbitrary units) ( ) ( =3,>150 cells). * <0.05, ** <0.01, and **** <0.0001 versus control using MannWhitney test",yes
PMC7649483,Figure_1,oa_package/3b/1e/PMC7649483.tar.gz,['Transcatheter aortic valve implantation.'],"Figure 1 Transcatheter aortic valve implantation. ( ) Angiography of the aortic root with the 6Fr Pigtail catheter. ( ) Predilatation of the native aortic valve annulus with 20mm non-compliant balloon. ( ) Positioning and partial expansion of the self-expandable, high-frame aortic bioprosthesis with intra-annular leaflet position (29mm, Portico Transcatheter Heart Valve, Abbott, Chicago, IL, USA). ( ) Final result after the deployment of aortic bioprosthesis with optimal implant position and without procedural complications. There was no paravalvular leak, no conduction disorders, nor significant transvalvular gradient detected peri- and post-procedurally.",yes
PMC5878329,Figure_4,oa_package/24/6e/PMC5878329.tar.gz,"['CT chest, abdomen and pelvis imaging did not reveal any evidence of malignancy but confirmed dislocation of the left humeral head with a reverse Hill-Sachs lesion and a reverse Bankart displaced glenoid fracture (figure 4).', 'Axial CT chest.']",Figure 4 AxialCT chest.Left humeral head posteriorly subluxed with reverse Hill-Sachs defect (arrowhead) and posteriorly displaced reverse Bankart lesion (arrow).,yes
PMC9403767,Figure_1,oa_package/52/db/PMC9403767.tar.gz,[],"Figure1 Liver distributed into individual slabs of 32 120 120. Disease was labeled as disease, regardless of the center of the slab. The validation and test set were not broken into slabs; our architectures accepted variable input sizes, with the entire liver being passed at once for evaluation and testing.",yes
PMC10949815,Figure_1,oa_package/f2/f5/PMC10949815.tar.gz,"[' 1A).', '\nPeri-operative imaging of the incarcerated dialysis catheter.', '(A) CT pre-assessment, (B) Fluroscopic balloon dilation, (C) Post-operative chest x-ray\nFollowing multidisciplinary team discussion between vascular, cardiothoracic and interventional radiology teams, a decision was made to undertake a second fluoroscopic technique using the Hong technique [7].', ' 1B).', ' 1C).', ' 1.']","Fig. 1 Peri-operative imaging of the incarcerated dialysis catheter. (A) CT pre-assessment, (B) Fluroscopic balloon dilation, (C) Post-operative chest x-ray",yes
PMC1526630,Figure_4,oa_package/a2/b3/PMC1526630.tar.gz,"['Immunohistochemistry and in situ hybridization of synovial tissues for BMP-4.', 'Histomorphological distribution of BMP-5 is comparable to that of BMP-4 ().']","Figure 4 Immunohistochemistry and hybridization of synovial tissues for BMP-4. In normal synovial tissue the expression of bone morphogenetic protein (BMP) is localized to the synovial lining layer. In rheumatoid arthritis (RA) and osteoarthritis (OA) tissue samples BMP-4 is expressed less by cells of the superficial synovial layer but more by cells scattered in deeper layers. Original magnifications: immunohistochemistry (IMH): RA, normal donors (ND) 40, OA 20; hybridization (ISH): RA, OA, ND 40.",yes
PMC5490176,Figure_3,oa_package/fe/da/PMC5490176.tar.gz,"['3a, Additional file 1: S4).', '3b), and a 1.', '3c).', '3d).', 'WD NSCs show increased lysosomal staining and lipid accumulation.', 'Statistical significance was calculated as each WD cell line compared against NCRM-1\nIn addition to lipid loading using FBS, loading with LDL was also tested.', '3).']",Fig. 3 WD NSCs show increased lysosomal staining and lipid accumulation. LysoTracker ( ) and nuclear ( ) staining images with quantitation of WD and control NSCs. LAMP2 ( ) and nuclear ( ) staining images with quantitation of WD and control NSCs. Nile red ( ) and nuclear ( ) staining images with quantitation of WD and control NSCs. Amplex red assay for cholesteryl ester content of WD and control NSCs. All data are displayed as meanSD. Statistical significance was calculated as each WD cell line compared against NCRM-1,yes
PMC6181940,Figure_1,oa_package/2f/79/PMC6181940.tar.gz,"[' 1a) showed transgene expression, which was suppressed in the absence of Dox (Dox ), whereas Dox treatment (Dox+) for 72 h resulted in the expression of either nuclear GFP-WT (', ' 1a, upper panels) or cytoplasmic GFP-NLSm proteins (', ' 1a, bottom panels).', 'Inducible cytoplasmic TDP-43 cell-based assay for screening of FTLD-TDP brain-extract seeding activity.', '01Using iGFP-WT and iGFP-NLSm cell lines, we evaluated whether TDP-43 intracellular aggregates can be induced when treated with sarkosyl-insoluble extracts containing pathological TDP-43 from FTLD-TDP postmortem frontal cortex (Supplementary ', ' 1b, left panel).', ' 1b, left panel, lane #2) whereas no phospho-immunoreactive bands were detected in non-pathological brain extracts (', ' 1b, left panel, lane #1).', ' 1b, right panel).', ' 1b, right panel).', ' 1b).', ' 1b, arrow).', ' 1c).', ' 1c), quantification of the percentage of area occupied by p409-410 immunostaining showed a significantly higher burden of TDP-43 aggregates in iGFP-NLSm cells compared with iGFP-WT cells (', ' 1d).', ' 1e).', ' 1e), while there were no significant differences between FTLD-TDP-C9+ and sporadic FTLD-TDP or FTLD-TDP-GRN.', ' 1f).']","Fig. 1 Inducible cytoplasmic TDP-43 cell-based assay for screening of FTLD-TDP brain-extract seeding activity. Representative photomicrographs of IF analysis to detect TDP-43 proteins (red and merge) in iGFP-WT and iGFP-NLSm cells in the absence of Dox (Dox) or after 72h of Dox treatment (Dox+). Expression of GFP-WT or GFP-NLSm proteins (green) is detectable after Dox treatment (Dox+). Cells were counterstained with DAPI to label the nuclei. Scale bar=20m. Immunoblot analysis of the sarkosyl-insoluble fraction from CTRL and FTLD-TDP brains used as a seeds (left panel, lanes #1 and #2, respectively) and sarkosyl-insoluble extracts from iGFP-WT and iGFP-NLSm cells (right panels) transduced with CTRL or FTLD-TDP seeds at 3 dpt. A C-terminal TDP-43 antibody (C1039, green) was used to detect total TDP-43 protein and the phospho-specific Ser409/Ser410 mAb (p409-410, red) was used for detection of the insoluble/pathological TDP-43. GFP-WT or GFP-NLSm were detected at ~69KDa, CTFs ~2520KDa, and endogenous TDP-43 at ~43KDa (arrow). Representative photomicrographs of IF analysis using the p409410 (red and merge) in iGFP-WT and iGFP-NLSm cells (green and merge) transduced with FTLD-TDP extracts at 3 dpt. Arrows point to p409410-positive aggregates. Cells were counterstained with DAPI to label the nuclei. Scale bar=20m. Plots show the percentage of area occupied by p409410 immunostaining in iGFP-WT and iGFP-NLSm cells at 3 dpt. Box-and-whisker plots show the median (solid line) and whiskers indicate the minimum and maximum values, with individual points representing independent experiments ( =4) overlaid as black circles. Unpaired two-tailed Student's test was used for the analysis. <0.05. In , plots show the quantification of the percentage of area occupied by p409-410 staining in iGFP-NLSm cells transduced with sporadic FTLD-TDP (red), FTLD-TDP-GRN (blue), FTLD-TDP-C9+ (green), and CTRL (white) extracts and the number of DAPI counts m in . In , , bar plots show mean and whiskers s.e.m., with individual points representing a different brain extract overlaid as black circles. A one-way ANOVA followed by a Tukeys multiple comparisons test was used for the analysis; in , treatment; =0.004, =7.08, degrees of freedom (DF)=3. <0.05 and <0.01",yes
PMC9463451,Figure_4,oa_package/ef/ce/PMC9463451.tar.gz,"[' 4a).', ' 4a).', 'Immunophenotyping through multiplexed staining correlates with RCM phenotypes.', 'Lymphocytes show strong association while tertiary lymphoid structures (TLS) show minimal association with TiME phenotypesTertiary lymphoid structures (TLS) are lymphoid formations that form in nonlymphoid tissues at sites of chronic inflammation and are associated with improved patient outcomes and response to immunotherapies31,32.', ' 4b).', ' 4a, b and 5b).', ' 4a).', ' 4a).']","Fig. 4 Immunophenotyping through multiplexed staining correlates with RCM phenotypes. Representative images from multiplexed IF analysis (CD8 , FOXP3, CD68 , PD-1 and PD-L1 ) on =24 BCC specimens show presence of CD8 T-cells, T-regs and macrophages in peritumoral infiltrates, along with PD-1 and PD-L1 expression in all three phenotypes: Inflam Vasc (black), Inflam Vasc (purple) and Inflam Vasc (pink). Most abundant numbers of CD8 cells ( -value= 0.031), PD1 cells ( -value = 0.036), and highest fraction of CD8 PD1 cells ( -value= 0.030) was found in the Inflam Vasc phenotype, indicating an inflamed but exhausted phenotype. Distribution of CD68 macrophages in intratumoral infiltrates was also highest in Inflam Vasc ( -value=0.055). Data are presented as column scatter plots and median analyzed with Kruskal-Wallis test adjusted for multiple comparisons using Dunns method. In peritumor analysis, =8, =7, =9 biologically independent specimens were analyzed from Inflam Vasc , Inflam Vasc and Inflam Vasc groups, respectively. In Intratumoral analysis, =6, =7, =8 biologically independent specimens were analyzed from Inflam Vasc , Inflam Vasc and Inflam Vasc groups, respectively. Dual IHC staining for tertiary lymphoid structures using CD3 T-cells (brown) and CD20 B-cells (pink) in =27 BCC specimens demonstrate abundance in the Inflam Vasc and lowest values in the Inflam Vasc groups ( -value = 0.039). No clear phenotypic association with TLS coverage was found ( -value = 0.988). Data are presented as column scatter plots and median and median analyzed with KruskalWallis test adjusted for multiple comparisons using Dunns method. In this analysis, =11, =7, =9 biologically independent samples were analyzed from Inflam Vasc , Inflam Vasc and Inflam Vasc groups, respectively. Source data are provided as a Source Data file. IF: immunofluorescence; IHC: immunohistochemistry.",yes
PMC5517616,Figure_6,oa_package/b5/23/PMC5517616.tar.gz,"[' 6, left column).', ' 6J).', ' 6B).', 'HUVECs were treated with total, EV-depleted and EV-enriched media from (A,B) first trimester, (C,D) second trimester, (E,F) preterm, (G,H) term and (I,J) PE placentas for 24 hr.', '\nTotal-conditioned media, but not EV-enriched media, demonstrated significant increase in leukocyte adhesion assay compared to vehicle control when prepared from both first trimester and sPE placental villi (first trimester: total media = 3.']","Figure 6 HUVECs were treated with total, EV-depleted and EV-enriched media from ( , ) first trimester, ( , ) second trimester, ( , ) preterm, ( , ) term and ( , ) PE placentas for 24hr.",yes
PMC11558486,Figure_3,oa_package/88/21/PMC11558486.tar.gz,['Calcifications of the costal cartilage.'],Figure 3 Calcifications of the costal cartilage. (A-C) Different calcifications of the costal cartilage in various patients (indicated by the arrows).,yes
PMC6800506,Figure_3,oa_package/68/db/PMC6800506.tar.gz,"[' 3a).', '3a, bottom panels).', 'A oligomers promote tau seeding in primary hippocampal neurons and human neuroblastoma cells.', '0001, one-way ANOVA)To quantitatively measure tau seeding in neuronal cells, we established a cellular assay in which human SH-SY5Y neuroblastoma cells were incubated with tau seeds with or without pre-treatment with A oligomers.', '3b).', '3c).']","Fig. 3 A oligomers promote tau seeding in primary hippocampal neurons and human neuroblastoma cells. 500nM tau seeds were added to primary hippocampal neurons from PS19 mice with (top panels) or without (bottom panels) 24h pretreatment of 200nM A oligomers. Immunostaining with tau antibodies HT7 (left) or AT8 (right) was performed 24h after the addition of tau seeds. Nuclei were stained with DAPI (blue). The scale bar denotes 20m. Tau inclusions are highlighted by white arrows. A schematic representation of the assays used to measure A promotion of tau seeding in neuroblastoma SH-SY5Y cells. Cells were treated with A oligomers at 24h and tau seeds at 48h and then lysed at 72h. ELISA was used to measure tau concentration in the lysates, then seeding was measured in the tau-biosensor cells, and the seeding capacity of the supernatant was measured by FRET-based flow cytometry. Quantification of tau seeding in SH-SY5Y cells with or without pretreatment with A oligomers. The representative western blot above the bar graph shows that a similar amount of total tau in the SH-SY5Y cell lysates was used in each case. Data are meanSD ( =6, **** <0.0001, one-way ANOVA)",yes
PMC5473003,Figure_4,oa_package/93/c8/PMC5473003.tar.gz,"['4a).', '4a).', '4a), indicating that demyelination is specifically related to -syn, and not a byproduct of the vector or GFP expression.', '4b).', '4b), consistent with the notion that oligodendroglial accumulation of -syn, but not GFP, causes disruption of oligodendrocyte function.', 'Demyelination in NHPs.', 'Scale bar 500 m\nMicroglial activation in response to Olig001-mediated -syn overexpression in NHPsActivation of microglia is an early pathogenic process in MSA, often associated with GCI formation.']","Fig. 4 Demyelination in NHPs. Representative images of Olig001--syn or Olig001-GFP injected monkeys stained with . Marked demyelination in the corpous callosum of Olig001--syn monkeys is shown by decreased staining ( ). The areas of demyelination correspond to phosphorylated Serine-129 -syn immunoreactivity in level matched sections ( ). No loss of myelin was observed in Olig001-GFP injected monkeys ( ), indicating that demyelination was a result of -syn overexpression and not a byproduct of the vector or GFP expression ( ). In the striatum, regions of phosphorylated Serine-129 -syn immunoreactivity ( ) associated with robust demyelination of internal capsule fibers and striasomes ( ), while GFP expression ( ) resulted in no changes in myelin ( ). 500m",yes
PMC6819398,Figure_1,oa_package/15/36/PMC6819398.tar.gz,"[' 1.', 'Schematic diagram of the two methods for performing imaging-guided bone biopsy (a-c).', 'c The demonstration of a CT-guided biopsy for bone lesions18F-FDG PET/CT-guided biopsy groupFirst, whole-body FDG PET/CT imaging was performed following intravenous injection of 222 370 MBq (6 10 mCi) 18F-FDG for all patients.']","Fig. 1 Schematic diagram of the two methods for performing imaging-guided bone biopsy ( ). The demonstration of a precise PET/CT-guided biopsy for bone lesions by guiding the needle placement in the metabolically active portion (red arrow) of the bone lesion. The demonstration of a false PET/CT-guided biopsy for bone lesions by guiding the needle placement in the reactive areas (red arrow) with necrosis or fibrosis, which may lead to a false-negative biopsy. The demonstration of a CT-guided biopsy for bone lesions",yes
PMC6601474,Figure_4,oa_package/43/a8/PMC6601474.tar.gz,"['AP activity was significantly higher in brain and serum but significantly lower in the gut and liver of the AD mice ().', 'After FSS treatment, AP activity in the brain and serum of the FSS-H mice and in the serum of the FSS-M mice were lower than in the AD mice ().', 'In contrast, the AP activity in the gut and liver of the FSS-H mice and FSS-M mice were higher than in the AD mice ().', 'However, changes in AP activity in the gut, liver, serum, and brain of the FSS-L mice were not particularly noticeable ().', '003""/>\nFSS regulates expression and activity of AP in the gut, liver, serum, and brain of APP/PS1 AD mice.']","Figure 4 . (a) Quantification of AP in the gut (n = 7 per group). (b) Quantification of AP in the liver (except WT group, n = 9; n = 8 per group). (c) Quantification of AP in the serum (except WT and AD group, n = 7; n = 6 per group). (d) Expression of AP protein from hippocampus tissue (n = 3) detected by western blot analysis and quantified in (e) compared with expression of GAPDH (36 kDa) as the control. Data represent the mean SEM. ## p < 0.05 and ### p < 0.001 compared with WT. P < 0.05, P < 0.01, and P < 0.001 compared with AD. FSS: Fo Shou San; AD: Alzheimer's disease; WT: wild type; FSS-L: 1.6 g/kg/day; FSS-M: 3.2 g/kg/day; FSS-H: 6.4 g/kg/day; AP: alkaline phosphatase.",yes
PMC5582519,Figure_1,oa_package/9b/c2/PMC5582519.tar.gz,"[""References1SikovWMBerryDAPerouCMSinghBCirrincioneCTTolaneySMImpact of the addition of carboplatin and/or bevacizumab to neoadjuvant once-per-week paclitaxel followed by dose-dense doxorubicin and cyclophosphamide on pathologic complete response rates in stage II to III triple-negative breast cancer: CALGB 40603 (Alliance)J Clin Oncol2015331321250927752HusainAAptakerLSpriggsDRBarakatRRGastrointestinal toxicity and Clostridium difficile diarrhea in patients treated with paclitaxel-containing chemotherapy regimensGynecol Oncol19987110410797843283TashiroMYoshikawaIKumeKOtsukiMIschemic colitis associated with paclitaxel and carboplatin chemotherapyAm J Gastroenterol200398231232125269804DanieleBRossiGBLositoSGridelliCde BellisMIschemic colitis associated with paclitaxelJ Clin Gastroenterol200133159160114684475KlauberNParangiSFlynnEHamelED'AmatoRJInhibition of angiogenesis and breast cancer in mice by the microtubule inhibitors 2-methoxyestradiol and taxolCancer Res199757818689880456IbrahimNKSahinAADubrowRALynchPMBoehnke-MichaudLValeroVColitis associated with docetaxel-based chemotherapy in patients with metastatic breast cancerLancet200035528128310675076Splenic flexure colitis.""]",Fig. 1 Splenic flexure colitis.,yes
PMC5306191,Figure_8,oa_package/4c/fd/PMC5306191.tar.gz,"[' 8).', '', '(2006), with permission\nGlycosulphation is a major feature of colonic mucins and is reduced in IBD and cancer.']","Fig.8 Loss of MUC2 and sulpho-Lewis in ulcerative colitis. Detection of MUC2 glycoprotein by immunostaining with the Lum23 antibody for normal ( ) and clinically severe UC ( ). The same specimens were also tested for MUC2 mRNA for normal ( ) and UC ( ). Immunostaining for MUC2 in mucosa adjacent to an ulcer ( ) showed reduced staining compared with normal intact mucosa ( ). Detection of sulpho-Le with the F2 antibody showed localization throughout the mucosa, including the mucus gel layer ( ). Sulpho-Le staining was preserved in mild colitis ( ), but was depleted at the luminal surface and upper crypts in severe colitis ( ). In situ hybridization samples were counterstained with toluidine blue and immunohistological sections with haematoxylin; from Longman et al. ( ), with permission",yes
PMC9793602,Figure_3,oa_package/d5/c0/PMC9793602.tar.gz,"[' 3a).', ' 3b and c).', ' 3d).', ' 3e).', 'Levels of peroxidation and antioxidant markers in mice of each group.', '01 compared with the Con groupQuercetin enhanced mitochondrial function in the GCs of POI miceTo further explore the antioxidant mechanism of quercetin, we measured mtDNA production, ATP production, and mitochondrial membrane potential (MMP) levels, which are effective indicators of mitochondrial function.']","Fig. 3 Levels of peroxidation and antioxidant markers in mice of each group. Serum levels of ( ) T-AOC, ( ) SOD, ( ) GSH-Px, ( ) MDA. ROS fluorescence intensity in mouse granulosa cells. T-AOC: total antioxidant capacity; SOD: superoxide dismutase; GSH-Px: glutathione peroxidase; MDA: malondialdehyde; ROS: reactive oxygen species. All data are expression as meanstandard deviation mean (xSD) ( =6). P<0.05 and <0.01 compared with the CTX group; <0.05 and <0.01 compared with the Con group",yes
PMC6251248,Figure_10,oa_package/8e/1d/PMC6251248.tar.gz,[],"Figure 10 HIV Sialadenitis. A 44-year-old female with HIV not on highly active antiretroviral therapy (CD4 109) presenting with 1 month of progressive painless bilateral parotid swelling. Axial computed tomography demonstrates heterogeneous attenuation throughout the parotid glands with prominent cystic lesions (white arrows), benign lymphoepithelial cysts.",yes
PMC11616600,Figure_5,oa_package/0a/60/PMC11616600.tar.gz,"['We chose a DEEP:PHI built-in model: Attention U-Net (see ).', 'Network architecture diagram for the proposed model (Attention U-Net).', '']","Fig. 5 Network architecture diagram for the proposed model (Attention U-Net). Attention U-Net is a model an architecture based on the U-Net model with added attention mechanisms. This model learns to suppress relatively less important areas in the input images while enhancing regions with important features. The model consists of 5 stages, with 32, 64, 128, 256, and 512 filters used in each stage. In the bottleneck section, 1024 filters are used. Additionally, to minimize overfitting, dropout layers are added after each max pooling operation, and batch normalization layers are added after each convolution operation.",yes
PMC5022524,Figure_3,oa_package/b5/52/PMC5022524.tar.gz,"['A steep Trendelenburg position allowed the small bowel and omentum to be moved away from the tumour, which was seen protruding through the descending and sigmoid mesocolon [a].', 'Multiple feeding vessels were clipped and divided [b].', 'Dotted circle denotes the position of the palpable tumour(a) Large tumour protruding into the descending mesocolon (b) Vessel entering the tumour from the inferior mesenteric artery being clippedDISCUSSIONParaganglia are the main source of catecholamine production in the foetus and regress after 3 years of age.']",Figure 3 (a) Large tumour protruding into the descending mesocolon (b) Vessel entering the tumour from the inferior mesenteric artery being clipped,yes
PMC6256820,Figure_3,oa_package/a5/f6/PMC6256820.tar.gz,"['5%, respectively ().', 'Protein expression of reprogramming factor Oct3/4 and pluripotency marker Nanog.']","Figure 3 Protein expression of reprogramming factor Oct3/4 and pluripotency marker Nanog. Cells were collected by centrifugation, followed by staining for Oct3/4 and Nanog. Cells were analyzed in a BD Accuri Plus Flow Cytometer (BD).
*p<0.05 compared to peripheral blood mononuclear cells.
PBMC: Peripheral blood mononuclear cell.",yes
PMC6923855,Figure_7,oa_package/a3/cb/PMC6923855.tar.gz,"[' 7) revealed variable presence of large numbers of PRBCs sequestered in small cerebral capillaries and venules in addition to haemozoin pigment deposition, perivascular haemorrhages, oedema and proliferation of microglial cells.', 'Photomicrographs are representative images from three experiments\nSimilarly, examination of sections from the liver of P.']","Fig.7 Microscopic observations of H&E staining on day 5 post infection in the brain of an uninfected control mouse and infected mice following the modulation of IL-35. Note the presence of intravascular lymphocytes (1), PRBC sequestration and formation of rosettes (2), perivascular haemorrhage (3), perivascular space enlargement (4), lymphocytes (5), pyknotic neurons (6) and pericytes (7). Images were acquired at 400 total magnification (Scale bar; 50m). Photomicrographs are representative images from three experiments",yes
PMC9299925,Figure_5,oa_package/29/ba/PMC9299925.tar.gz,[],"Figure 5 Summary of the assessments of average dermal value, hypodermal, and muscularis fascia thickness by highfrequency ultrasound (a), Localized Scleroderma Cutaneous Assessment Tool (LoScat) (b), and of the Dermatology Life Quality Index (DLQI) (c)",yes
PMC7729318,Figure_3,oa_package/40/c1/PMC7729318.tar.gz,"['CDC7 is verified as a functional target of miR-29bThe protein expression of CDC7 was decreased by miR-29b mimic but increased by miR-29b inhibitor, as shown in A (P 0.', 'The results showed that CDC7 might be a potential target of miR-29b (B).', 'Therefore, the luciferase activity in miR-29b mimic and CDC7 wildtype co-transfected cells was significantly reduced compared to mutant CDC7 or scrambled RNA transfected cells (C, P 0.', 'Consistent with former studies, we showed inhibition of migration and proliferation with miR-29b mimic, through significantly inhibited Ki67 and CDC7 (D, P 0.', 'CDC7 was verified as a functional target of miR-29b.']","Figure 3 CDC7 was verified as a functional target of miR-29b. HUASMCs were transfected with miR-29b scramble, miR-29b mimic, or miR-29b inhibitor, then kept for another 24 hours for later experiments. (A) The protein expression of CDC7 was verified by western blot assay. *, P<0.05 and **, P<0.01 compared with the miR-29b scramble group. (B) TargetScan online prediction of combination between CDC7 and miR-29b. HEK 293T cells were co-transfected with CDC7 WT, CDC7 MUT, and miR-29b mimic, and (C) the target relationship between CDC7 and miR-29b was confirmed by Luciferase reporter assay. *, P<0.05, compared with the CDC7 WT group. HUASMCs transfected with miR-29b scramble, miR-29b mimic, or pc-CDC7. (D) The protein levels of CDC7 and Ki67 were verified by western blot assay. *, P<0.05, compared with the scramble group; , P<0.05, compared with the pc-CDC7 group. CDC7, cell division cycle 7-related protein kinase; HUASMC, human umbilical artery smooth muscle cells; WT, wild-type; MUT, mutant; miR-29b, microRNA-29b.",yes
PMC3427179,Figure_2,oa_package/fa/a7/PMC3427179.tar.gz,"['Control cells or cells treated with DIDS alone excluded propidium iodide (PI, n = 4 6 for each treatment, A D) and trypan blue (TB, n = 3 5 for each treatment, ', '2E), and did not release AK (n = 3 for each treatment, F); whereas pathology-challenged cells took up PI and TB, and IS- or STS-treatment caused 60 90% of total cellular AK to be released from neurons at 24-hrs.', 'None of the treatments examined had a significant effect on plasma membrane widths (n = 10 for each, G).', 'g002DIDS abolishes pathology-induced membrane degradation.']",10.1371/journal.pone.0043995.g002,yes
PMC4123273,Figure_2,oa_package/f7/3b/PMC4123273.tar.gz,[],"Figure 1 (l-s) (l) A 44-year-old female with granulomatous uveitis secondary to sarcoidosis (wide angle lens (left side), and high magnification lens (right side)) (m) A 78-year-old male with pterygium (wide angle lens) (n) A 42-year-old male with vascularized corneal scar secondary to trauma occurred 35 years ago (wide angle lens) (o) A 49-year-old female with pinguecula (high magnification lens) (p) A 47-year-old male with conjunctival nevus (high magnification lens) (q) A 75-year-old female with conjunctival primary acquired melanosis (high magnification lens) (r) A 45-year-old female with lymphangiectasia (high magnification lens) (s) A 70-year-old female with subconjunctival hemorrhage (high magnification lens)",yes
PMC3015928,Figure_1,oa_package/17/02/PMC3015928.tar.gz,"['A CT scan revealed a 4-cm dilated duodenal bulb with abrupt decompression to normal duodenum beyond the SMA ().', '.']","Figure 1. Computed tomography of the abdomen revealed dilated proximal duodenum (large white arrow), decompressed distal duodenum (large black arrow), and narrow distance between the superior mesenteric artery and aorta (small double arrow).",yes
PMC11082483,Figure_6,oa_package/e7/3d/PMC11082483.tar.gz,"['0 15 mm NC Euphora balloon in the mid and proximal segments of LAD ((a) (c)).', '.', '1177_20480040241248924-fig6"" position=""float""/>CP: coronary perforation; IVUS: intravascular ultrasonography; DES: drug-eluting stent; KB: kissing balloon technique; LAD: left anterior descending artery; LMCA: left main coronary artery; RCA: right coronary artery.']","Figure 6. (a) Angiographic control with a TIMI III flow, however, IVUS showed DES underexpantion, so postdilatation was decided. (b, c) Postdilatation was performed with a 3.015mm NC Euphora balloon in the mid and proximal segments of LAD. (d, e) On angiographic control, a contrast extravasation jet (blue arrow) was seen through a>1mm orifice, making the diagnosis of an Ellis type III CP. Immediately, the CP was sealed by prolonged low-pressure insufflation and protamine was administered. (F) After awaiting 15min, angiographic control showed complete sealing of the CP and absence of residual leak (blue arrowhead).",yes
PMC9409200,Figure_6,oa_package/9c/b4/PMC9409200.tar.gz,"['Fer-1 Abrogates Diabetes-Induced Activation of HMGB1 and Increase in Inflammatory Cytokines presents the results of immunohistochemical detection of HMGB1 (a, b, and c) and analysis of protein content of TNF- (d) and IL-6 (e).', 'Microscopic observations were confirmed quantitatively since statistically significant differences in overall tissue and nuclear HMGB1 immunopositivity were noted among the experimental groups (b,c).', 'Protein content of TNF- (d) was significantly increased in the DM group compared to the control (p 0.', 'Although the changes in the amount of IL-6 protein are in the same direction as TNF- , they only showed a trend of increase in the DM and a trend of decrease in the diabetic group treated with Fer-1 (e).', 'HMGB1, TNF- , and IL-6 in liver tissue of control (Ctrl), diabetic (DM), and diabetic Fer-1-treated (DM + Fer-1) animals.']","Figure 6 HMGB1, TNF-, and IL-6 in liver tissue of control (Ctrl), diabetic (DM), and diabetic Fer-1-treated (DM + Fer-1) animals. ( ) Immunohistochemical detection of HMGB1; scale bars50 m. ( ) Quantification of tissue and ( ) nuclear immunopositivity of HMGB1. Protein level of TNF- ( ) and IL-6 ( ) measured by ELISA. Values presented as means SEM. Statistical significance: compared with the Ctrl group (*), *** < 0.001. DM vs. DM + Fer-1 comparison (#), ### < 0.001.",yes
PMC6243870,Figure_5,oa_package/a6/da/PMC6243870.tar.gz,"['At postoperative day 7, the quantity of collagen fibers increased in both groups, though they were more numerous and more ordered in the HBO group (A, 5B).', 'Images of tissue Masson-stained sections from the HBO group (A) and control group (B) on postoperative day 7.']","Figure 5 Images of tissue Masson-stained sections from the HBO group ( ) and control group ( ) on postoperative day 7. Image at 200 magnification. Collagen fibers were more abundant than at earlier time points in the control group, but still less abundant than in the HBO group.",yes
PMC9605814,Figure_4,oa_package/1e/74/PMC9605814.tar.gz,[' CT scan of the head 24 hours later after treatment with IV corticosteroids.'],Figure 4 CT scan of the head 24 hours later after treatment with IV corticosteroids. Significantly improved gray-white matter differentiation. No acute intracranial abnormality is noted.,yes
PMC9767790,Figure_1,oa_package/46/b2/PMC9767790.tar.gz,"['(A) Dystrophy corneal; (B) Verrucous carcinoma on the tongue tip; (C) Alopecic plaque, an ulcerated oozing mass alternated with small masses on the scalp; (D) Hyper and hypochromic macules disseminated on the whole body; (E) Actinic keratosis lesions.']","Figure 1 (A) Dystrophy corneal; (B) Verrucous carcinoma on the tongue tip; (C) Alopecic plaque, an ulcerated oozing mass alternated with small masses on the scalp; (D) Hyper and hypochromic macules disseminated on the whole body; (E) Actinic keratosis lesions.",yes
PMC9908008,Figure_4,oa_package/e8/cc/PMC9908008.tar.gz,"['In TBSS analyses of metrics from the NODDI model, NDI decreased with DWMA volume throughout portions of all major white matter regions with the exception of the cerebellum (), in concordance with the FD metric, both of which should approximate the same underlying truth.', '.']",Fig. 4. Relationship between NODDI metrics and DWMA extent. TBSS results showing white matter regions in which ODI (top two rows) and NDI (bottom row) decreased with DWMA extent. Green areas represent the TBSS white matter skeleton.,yes
PMC4602622,Figure_6,oa_package/54/c1/PMC4602622.tar.gz,[],"FIGURE 6 (A) TTC7A was expressed in intestinal epithelial cells and endothelial cells in controls and highly expressed in HMIA patients (F, P1, and P2). (B) TTC7A was expressed in normal epithelial cells from pancreas, in both exocrine and endocrine glands, and highly expressed in HMIA pancreas. HMIA=hereditary multiple intestinal atresia, TTC7A=tetratricopeptide repeat domain7A.",yes
PMC2776973,Figure_4,oa_package/29/d6/PMC2776973.tar.gz,"['g004Cys4p(1 353) accelerates initiation of cell division.', 'g004""/>We found that CYS4(1 353) cells have a shorter G1 than their otherwise isogenic wild type (CYS4 ) counterparts ().', ""25, based on a 2-tailed Student's t test) (A)."", 'We obtained essential identical values as before, demonstrating that the accelerated growth rate of these cells is due to the CYS4(1 353) allele ().', 'In conclusion, introduction of a single hypermorphic allele of a sulfur metabolic enzyme, cystathionine- -synthase, is sufficient to accelerate growth rate and initiation of cell division ().', 'From these graphs we determined the rates reported in , calculated as described in S3 Fig(A) Accompanying FACS plot data for .', '(B) Multiple FACS scatter plot data frames to accompany C.']",10.1371/journal.pone.0256166.g003,yes
PMC8402792,Figure_2,oa_package/bb/7f/PMC8402792.tar.gz,"['These studies demonstrated that non-neuronal CNS cells such as astrocytes could also play important roles in inducing Alzheimer s-like pathology in HAND ().', 'Role of central nervous system cell types in HIV-mediated accumulation of amyloids: both HIV and HIV-infected cells cross the blood brain barrier and infect glial cells in the central nervous system.']","Figure 2 Role of central nervous system cell types in HIV-mediated accumulation of amyloids: both HIV and HIV-infected cells cross the bloodbrain barrier and infect glial cells in the central nervous system. Activated neurons, microglia, astrocytes, pericytes, and endothelial cells play a key role in the accumulation of amyloids. Prolonged accumulation of amyloids, in turn, promotes neurodegeneration.",yes
PMC8377834,Figure_2,oa_package/93/8d/PMC8377834.tar.gz,"[' 2).', 'Comparison of the original image a with the same image in which the sharpening was applied b reveals radiolucent rim formation around fillings, crowns, and implantsThe main factor of these changes is an effect called halo, rebound or Uberschwinger artifacts [4], which is a radiolucent rim on the transition border between structures with more and less pronounced densities (', ' 2).']","Fig. 2 Comparison of the original image with the same image in which the sharpening was applied reveals radiolucent rim formation around fillings, crowns, and implants",yes
PMC9547750,Figure_3,oa_package/11/b5/PMC9547750.tar.gz,"['A, marked mucosal thickening of the left maxillary and frontal sinus is noted in Coronal T2-weighted image B, Axial T2-weighted image shows hypointense fungal elements along with intraconal fat obliteration in the left orbit C, Axial T1-weighted image, involvement of the left orbital apex with extension to the left cavernous sinus D, Fat-suppressed axial T1-weighted post-contrast image shows heterogenous enhancement of left orbital components E, F, diffusion restriction in the left orbit on DWI/ADC images with extension through the orbital apex to the left cavernous sinus, compatible with left cavernous sinus obliterationCRAO was the most common significant intraocular pathologic finding, detected in 37 patients (28%).']","Fig. 3 Orbital MRI of a 58-year-old male patient with left Covid-associated rhino-orbito-cerebral mucormycosis. , marked mucosal thickening of the left maxillary and frontal sinus is noted in Coronal T2-weighted image , Axial T2-weighted image shows hypointense fungal elements along with intraconal fat obliteration in the left orbit , Axial T1-weighted image, involvement of the left orbital apex with extension to the left cavernous sinus , Fat-suppressed axial T1-weighted post-contrast image shows heterogenous enhancement of left orbital components , , diffusion restriction in the left orbit on DWI/ADC images with extension through the orbital apex to the left cavernous sinus, compatible with left cavernous sinus obliteration",yes
PMC4841328,Figure_2,oa_package/6a/38/PMC4841328.tar.gz,"['The CT taken on the following day () showed no significant remaining hematoma; there were signs of ischemic damage in the surrounding brain tissue, but the cerebral vasculature was open without vasospasms.', 'CT control one day after surgery.']",Figure 2 CT control one day after surgery.,yes
PMC11503032,Figure_2,oa_package/85/d2/PMC11503032.tar.gz,"[' and provide examples of synovial thickness and joint effusion grading.', 'Case example of MRI assessment of a knee with tumour invasion.', 'R2-galleyfig2"" position=""float""/>Case example of MRI assessment of a hip with tumour invasion.']",Fig. 2 Case example of MRI assessment of a knee with tumour invasion. A 58-year-old female presented with left distal femur osteosarcoma and intra-articular extension on pathology. a) Axial T2FS image shows grade 1 knee joint effusion (arrows) in the medial patellofemoral recess and posterior to posterior cruciate ligament. b) Axial T1FS postcontrast shows grade 2 synovial thickening (line) measuring 3 mm in the medial patellofemoral recess.,yes
PMC4569792,Figure_1,oa_package/6b/2f/PMC4569792.tar.gz,"['The next ultrasound, performed by the same radiologist one year later, showed a 21 mm vegetating bladder lesion, close to the urethra ().', '0-3594898451917947491Ultrasound showing a 21 mm vegetating lesion on the bladder neck.']",Figure 1 Ultrasound showing a 21mm vegetating lesion on the bladder neck.,yes
PMC6032039,Figure_4,oa_package/9a/80/PMC6032039.tar.gz,"['The presence of these parasites seems to produce a disengagement of these two structures and creating empty spaces () or vesicles.', '', 'Typical structures recognized, such as oral sucker, pseudosuckers, intestinal caeca and also digested material were commonly seen within the metacercaria (, , ).']",Fig. 3 Normal aspect of the retina in Arctic charr with different layers. From the eye exterior to the eye interior: (RP): Retinal pigment epithelium. (RC) Cones and rods layer. (ON) Outer nuclear layer. (OP) Outer plexiform layer. (IN) Inner nuclear layer (IP) Inner plexiform layer. (GC) Ganglion cell layer. (GA) Axons of the ganglion layer. Scale bar=300m.,yes
PMC7452828,Figure_3,oa_package/ce/40/PMC7452828.tar.gz,"['DIC Virus agent at point 1 Others agents at point 1 Platelet aggregation Fibrin deposition Thrombotic occlusion Acute infarction Heart failure Multi-organ failure DeathIMA.', 'Also in this case the differential diagnosis with identification of the agent is not easy since there is often no evidence of the virus in the myocardium and the morphological changes are the indirect result of the damage caused which manifests itself as inflammatory infiltrate in myocarditis, with necrosis in IMA and with increased state of coagulability and thrombosis in DIC.']",Fig. 3 IMA. Areas of coagulative necrosis of the myocardium as initial hypoxic damage. a. HIV (H&E stain x20). b. Coronavirus (H&E stain x20).,yes
PMC9908008,Figure_3,oa_package/e8/cc/PMC9908008.tar.gz,"['In the connectivity-based fixel enhancement analysis, FD decreased with DWMA extent throughout the majority of the white matter (), although not in the cerebellum.', '.']","Fig. 3. Relationship between CSD metrics and DWMA extent Streamlines traversing fixels in which CSD metrics FD, FC, and FDC decreased with DWMA extent. For FD and FDC, all significant regions are shown at once, i.e., the image of the significant streamlines is not dependent on the position of the crosshair. For rows FC 1 and FC 2, the crosshair shows the voxel position on two different views of the same data. The color bar indicates the p-value after family-wise error rate correction.",yes
PMC9577858,Figure_1,oa_package/2e/93/PMC9577858.tar.gz,"['Dilated fundoscopy of the right eye showed a large circumscribed subhyaloid hemorrhage covering the macular region with a horizontal upper level and an arciform lower limit ().', 'Color photo fundus demonstrates a large subhyaloid hemorrhage covering the macular region with a horizontal upper level and an arciform lower limit.', ')Swept source OCT revealed shadow effect of the preretinal hemorrhage covering the macula, obstructing the image of the underlying retina and subretinal space (']","Fig. 1 Color photo fundus demonstrates a large subhyaloid hemorrhage covering the macular region with a horizontal upper level and an arciform lower limit. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)",yes
PMC4886526,Figure_3,oa_package/4e/96/PMC4886526.tar.gz,"['1 mM NAD+ and 300 M H2O2 compared with that in RPE cells treated with 300 M H2O2 alone (A,B).', '1 mM NAD+ and 300 M H2O2 compared with that in RPE cells treated with 300 M H2O2 alone (A,C).', 'In the present study, we showed that NAD+ treatment significantly decreased not only intracellular ROS level but also intranuclear ROS level induced by H2O2 ().', 'org/1999/xlink"" xlink:href=""srep26322-f2""/>NAD+ reduced ROS level in the cultured RPE cells induced by H2O2.']","Figure 3 NAD reduced ROS level in the cultured RPE cells induced by H O . ( ) Representative high content screening images for the ROS level stained by deep red in the cultured RPE cells treated without H O (Con) or alone with 300M H O (H O ), or with 300M H O and 0.1mM NAD (H O NAD ) for 24hours. Nuclei were labeled with Hochest 33342. Scale bar equals 100m. ( ) Statistical data from ( ) showing the intracellular ROS level. ( ) Statistical data from ( ) showing the intranuclear ROS level. Data are expressed as meanSEM from at least three independent experiments; *P<0.05, **P<0.01, ***P<0.001.",yes
PMC10630163,Figure_4,oa_package/59/70/PMC10630163.tar.gz,['01) (; Supplementary Table S3).'],"FIGURE 4 The effects of hUC-MSC administration on #NEUT, %NEUT, #LYMPH, %LYMPH, and WBC counts in cynomolgus monkeys. * 0.05, ** 0.01, vs. the control group.",yes
PMC5351652,Figure_4,oa_package/45/3c/PMC5351652.tar.gz,"['The expression of EMT related proteins were measured by western blot analyses after transfectionDISCUSSIONDespite the great therapeutic advances made in RCC, including surgical resection and adjuvant therapy, the long-term prognosis of RCC patients with distant metastases remains unfavorable.']",Figure 4 The expression of EMT related proteins were measured by western blot analyses after transfection,yes
PMC9600253,Figure_2,oa_package/31/77/PMC9600253.tar.gz,"['Pathological Characteristics of Four ECs Involving ECPCase 1: A 17 mm sized ECP (A), with a long fibrovascular stalk and elongated endocervical glands, was involved by pleomorphic EC cells.', 'The largest dimension of EC involving ECP was 6 mm, with an invasion depth of 1 mm into the polyp stroma (B).', 'Poorly differentiated carcinoma involving the surface and stroma of ECP displayed solid and cribriform architecture, compatible with grade 3 EC (C).', 'Low-power magnification of the ovarian tumor revealed a definite involvement of the ovarian surface (D).', 'The primary ovarian EC showed high-grade architectural and cytological atypia, which was compatible with a grade 3 EC diagnosis (E).', 'A few microscopic areas resembling clear cell carcinoma were also noted (F).', 'The expressions of ER (H) and PR (I) were focal but strong in the tumor cell nuclei.', 'Solid and cribriform architecture, high-grade nuclear atypia, and a complete lack of p53 protein expression (J) were compatible with grade 3 EC.', 'Negative WT1 immunoreactivity (K) excluded the possibility of high-grade serous carcinoma.', 'Grade 3 ovarian EC involving ECP: case 1.']","Figure 2 Grade 3 ovarian EC involving ECP: case 1. ( ) A 1.7 cm pedunculated polyp arises from the endocervix. ( ) Complex glandular proliferation involves the surface and stroma of ECP. The invasion depth measures 0.1 cm. ( ) High-power magnification reveals poorly differentiated carcinoma displaying solid and cribriform architecture and high-grade cytological atypia. ( ) The ovarian mass involves the surface and destructively invades the stroma. ( ) The degree of cytological atypia and architecture are similar to those of poorly differentiated carcinoma involving ECP. ( ) A few microscopic areas resembling clear cell carcinoma (blue asterisks) are noted in the ovarian tumor. ( ) The sternal metastatic tumor invades the bony trabeculae and exhibits poorly differentiated carcinoma, and its histological features are similar to those of EC involving ECP (image ( )) and ovary (image ( )). ( ) Immunohistochemical staining reveals focal and strong positivity for ( ) estrogen receptor and ( ) progesterone receptor and ( ) complete absence of p53 protein expression. ( ) Negative Wilms tumor 1 immunoreactivity excludes the possibility of serous carcinoma. ( ), Hematoxylin and eosin staining; ( ), immunohistochemical staining with polymer method. Original magnification: ( ), 4; ( ), 40; ( ), 100; ( ), 20; ( ), 100; ( ), 200; ( ), 40; ( ), 60; ( ), 60; ( ), 60; ( ), 80.",yes
PMC4511625,Figure_2,oa_package/b6/cd/PMC4511625.tar.gz,['Female patient aged three years and three months: preoperative X-ray in June 2004 (A).'],"Fig. 2 Female patient aged three years and three months: preoperative X-ray in June 2004 (A). X-ray in January 2005, three months after operation on left hip and six months after operation on right hip (B). X-ray in October 2011, in the late postoperative period, seven years after operation on right hip and six years and nine months after operation on left hip, presenting type 4 necrosis of the femoral head (C).",yes
PMC10451229,Figure_1,oa_package/7f/88/PMC10451229.tar.gz,"['45 SD glomeruli per field was observed in Group E ().', 'II1681735573Morphometrical study of the kidney; examples of glomeruli (arrow) identified in histological sections of dogs from Group A (A), Group B (B), Group C (C), Group D (D), and Group E (E), respectively.']","Figure 1 Morphometrical study of the kidney; examples of glomeruli (arrow) identified in histological sections of dogs from Group A ( ), Group B ( ), Group C ( ), Group D ( ), and Group E ( ), respectively. Hematoxylin and Eosin (original magnification 40). Mean values of the number of glomeruli were 9.48 2.99 SD for animals in Group A, 5.6 0.5 SD for animals in Group B, 4.4 0.33 SD for animals in Group C, 3.6 0.96 SD for animals in Group D, and 1.9 0.45 SD for animals in Group E.",yes
PMC6417362,Figure_1,oa_package/3e/b4/PMC6417362.tar.gz,"['The number of callosal and pericallosal lesions was 20 [Table 2 and ], that of thalamic and basal ganglia located lesions was 21 [Table 2 and ], that of multifocal lesions was 20 [Table 2 and ], that of lobar lesions was 15 [Table 2 and ], and that of brainstem lesions was 7 [Table 2 and ].', 'Table 2Distribution of the Cerebral Lesions on Radiological AreasHistopathlogy of Callosal and Pericallosal LesionsCase No (20)%24 GBM (grade 4)11 Lymphoma4 Anaplastic astrocytoma (grade 3)3 Subependymal giant cell astrositoma (grade 1)1 Nonspecific1Histopathlogy of Thalamic and Basal Ganglia LesionsCase No (21)%25,3 GBM (grade 4)4 Anaplastic astrocytoma (grade 3)4 Astrocytoma (grade 2)4 Lymphoma3 Anaplastic oligodendroglioma (grade 3)1 Low grade glial tumor (grade 2)1 Gliomatosis cerebri (grade 3)1 Abscess1 Infarct1 Biopsy negative1Histopathlogy of Multifocal LesionsCase No (20)%24 Lymphoma4 GBM (grade 4)3 Metastasis2 Encephalitis2 Anaplastic oligodendroglioma (grade 3)1 Vasculitis1 Small round cell tumor1 Reaktive gliosis1 Necrosis1 Histiocytosis1 Demyelinating plaque1 Nonspecific1 Biopsy negative1Histopathlogy of Lobar LesionsCase No (15)%18 GBM (grade 4)4 Anaplastic astrocytoma (grade 3)2 Astrocytoma (grade 2)2 Histiocytosis2 Lymphoma1 Metastasis1 Cerebritis1 Low grade glial tumor1 Toxoplasmosis1Histopathlogy of Brain Stem LesionsCase No (7)%8,4 GBM (grade 4)2 Anaplastic astrocytoma (grade 3)1 Atypical pilocytic astrocytoma (grade 2)1 Neuroepithelial cyst1 Metastasis1 Infarct1 Total83%100(a) Grade 4 glial tumor (glioblastoma multiforme), (b) lymphoma, (c) Grade 1 glial tumor (subependymal giant cell astrositoma)(a) Abscess, (b) Grade 2 glial tumor (astrocytoma), (c) infarct, (d) Grade 3 glial tumor (anaplastic astrocytoma)(a) Histiocytosis and (b) cerebritis(a) Small round cell tumor, (b) histiocytosis, (c) metastasis(a) Grade 4 glial tumor (glioblastoma multiforme), (b) Grade 2 glial tumor (atypical pilocytic astrocytoma), and (c) neuroepithelial cystFor the four cases, histopathological diagnoses of the lesions could not be made: two of which had negative biopsy samples normal brain tissue and two had nonspecific lesions.']","Figure 1 (a) Grade 4 glial tumor (glioblastoma multiforme), (b) lymphoma, (c) Grade 1 glial tumor (subependymal giant cell astrositoma)",yes
PMC3112200,Figure_13,oa_package/95/56/PMC3112200.tar.gz,[],10.1371/journal.pone.0020501.g013,yes
PMC5459995,Figure_7,oa_package/22/62/PMC5459995.tar.gz,"[', a,b), whereas in patients at prodromal or early stage (grades 1 2) there was no significant change (Supplementary ', ', a,c).', 'Indeed, in R6/1 mice we observed changes similar to those in Pyk2 mutant mice, including a decrease in total GluN2A but no change in GluN2B, a decrease in tyrosine phosphorylated GluN2A and GluN2B, and a marked decrease in total PSD-95 (d,e).', 'Double immunostaining for Pyk2 and PSD-95 in CA1 of wild-type and R6/1 mice showed a decreased number of PSD-95-positive puncta in R6/1 mice as compared to wild-type mice (f,g), and less co-localization of PSD-95-positive and Pyk2-positive puncta in mutant mice (', 'Pyk2 partly rescues the hippocampal phenotype of R6/1 miceSince the levels of Pyk2 in the hippocampus of HD patients and R6/1 mice (a c) were close to those in Pyk2 / mice, which displayed a similar phenotype, we asked whether correcting this defect in R6/1 mice could rescue some of their deficits.', '8k) compared to WT-GFP mice, consistent with the results shown in g i.', 'org/1999/xlink"" xlink:href=""ncomms15592-f6""/>Hippocampal alterations of Pyk2 and synaptic markers in Huntington\'s disease.']","Figure 7 Hippocampal alterations of Pyk2 and synaptic markers in Huntington's disease. ( ) Hippocampal post-mortem samples from human patients grades 34 (HD3-4) and controls (Cnt., top panel) and from wild-type (WT) mice and R6/1 transgenic mice (lower panel) were analysed by immunoblotting for Pyk2 and -tubulin as a loading control. Molecular weight marker positions are indicated in kDa. ( ) Densitometric quantification of results as in , for human samples expressed as a percentage of the mean in controls ( =6 per group, Student's -test, =2.25, <0.05). ( ) Quantification of results as in for WT and R6/1 mice (percentage of WT mean, =46 mice per group, Student's -test, =3.23, =0.012). ( ) Immunoblotting for phosphorylated forms and total GluN2A and GluN2B, and PSD-95 in hippocampus of WT and R6/1 mice. ( ) Quantification of results as in (percentage of WT mean), Student's -test, pY1246-GluN2A, =3.10, =0.013, pY1325-GluN2A, =2.37, =0.04, GluN2A, =5.21, =0.0006, pY1472-GluN2B, =3.64, =0.0066, GluN2B, =1.22, =0.26, PSD-95, =9.18, <10 . ( ) Confocal images of the of CA1hippocampal sections from WT and R6/1 mice immunolabelled for PSD95 (red) and Pyk2 (green). Scale bar, 10m. ( , ) Quantification of results as in in (three slices per mouse, fivesix mice per genotype. ( ) Number of PSD95-positive puncta, Student's -test, =3.98, =0.003. ( ) Number of Pyk2/PSD-95-double-positive puncta, expressed as a percentage of WT mean, Student's -test, =4.66, =0.0009. All data are means+s.e.m. * <0.05, ** <0.01 and *** <0.001. R6/1 mice were 5-month old. Uncropped blots for and are shown in , respectively.",yes
PMC9569960,Figure_7,oa_package/5f/c3/PMC9569960.tar.gz,"['We created tau variants with serine or threonine residues mutated to aspartic acid at multiple phosphorylation sites that are reported to regulate tau-microtubule interaction, T231D/S235D and T231D/S235D/S262D, to mimic tau phosphorylation at these sites (A).', 'Double or triple pseudo-phosphorylation led to an increased level of both total and phosphorylated tau in cell lysates, as determined by Western analysis (B), suggesting site-specific phosphorylation of tau induced tau accumulation.', 'In contrast, a minimal effect on total tau secretion relative to wild type 0N4R was observed, although the triple pseudo-phosphorylation variant (S262D-containing) was found to promote oligomeric tau secretion to higher levels than 0N4R or T231D/S235D tau variant in SH-SY5Y cells (C).', 'To further evaluate the role of Ser262 pseudo-phosphorylation alone in tau secretion and intracellular tau aggregation, the cell lysates and culture supernatants were compared for SH-SY5Y cell cultures transfected with wild type tau or the S262D tau variant (D,E).', 'Similar to the results for the triple pseudo-phosphorylation variant, the S262D transfected group showed higher levels of tau oligomers in cell lysates compared to wild type 0N4R tau, based on sandwich ELISA measurements (D).', 'Extracellular secretion of total and oligomeric tau (E) was also higher in S262D tau compared to wild-type 0N4R, perhaps as a result of a reduced affinity of the S262D tau variant for microtubules described in prior reports [18], leading to higher levels of free cellular tau.', 'S262 phosphorylation increases tau secretion.']","Figure 7 S262 phosphorylation increases tau secretion. ( ) Schematic illustration of tau variants created via site-directed mutagenesis of wild type 0N4R construct by substituting serine at multiple phosphorylation sites (blue circles with P as indicated) into aspartic acid: (1) T231D/S235D; (2) T231D/S235D/S262D; (3) S262D. The residues are numbered in blue based on the full-length tau isoform, 2N4R, with regions of interest as noted by colored boxes. The amino acid sequences around these sites are also included using one letter abbreviation and the wild-type amino acid in blue, the change to aspartic indicated in red. P1 and P2 represent the proline-rich domains; R1R4 and R indicated microtubule binding repeat domains. ( ) Cell lysates from SH-SY5Y cells transfected with 0N4R tau wild-type (0N4R), T231D/S235D, or T231D/S235D/S262D, were analyzed by immunoblotting against the total tau antibody, HT7 and the phosphorylated tau antibody, AT8. ( ) ELISA assay was used to measure the total tau, HT7, and oligomeric tau, T22, present in the conditioned medium collected from these three groups, three days following transient transfection in SH-SY5Y cells. 0N4R tau (0N4R; unshaded), T231D/S235D (light gray), or T231D/S235D/S262D (dark gray) (* represents < 0.05 for 0N4R compared with S262D tau; = 3) ( ). Oligomeric tau was measured by sandwich ELISA using T22 and HT7 from the cell lysates of SH-SY5Y cells transfected with wild type 0N4R tau (unshaded) or the S262D variant (dark gray) (* represents < 0.05 for 0N4R compared with S262D tau; = 3). ( ) The total tau and oligomeric tau in the conditioned medium were measured by indirect ELISA using HT7 or T22 to investigate the effects of Ser262 phosphorylation alone on tau secretion. 0N4R tau (unshaded); S262D variant (dark gray) (* represents < 0.05 for 0N4R compared with S262D; = 3; ns = not significant).",yes
PMC8276686,Figure_2,oa_package/5f/b6/PMC8276686.tar.gz,"[' 2).', 'a, b Conventional postpuncture hemostasis protocol using a pneumatic pressure bracelet, and c, d shortened hemostasis protocol using proprietary hemostatic pad with a pneumatic pressure bracelet, both placed before the withdrawal of the introducerIf bleeding or hematoma is seen after 10 min of compression, we inflate the balloon for another 10 min and check afterwards if there is no hematoma to complete the protocol as describe above.', ' 2).']","Fig. 2 Conventional postpuncture hemostasis protocol using apneumatic pressure bracelet, and shortened hemostasis protocol using proprietary hemostatic pad with apneumatic pressure bracelet, both placed before the withdrawal of the introducer",yes
PMC7431901,Figure_5,oa_package/aa/b2/PMC7431901.tar.gz,"[' 5a-e, o).', '5c).', '5d).', '5o) and less in type I and type II pneumocytes (', '5e).', 'Comparative pulmonary histologic lesions in AGMs infected with SARS-CoV-2.', 'Images captured at 20x (m, d, i, n, j) and 40x (c, h, e, o)Histologically, all three AGMs euthanized at 34 dpi developed moderate multifocal chronic interstitial pneumonia ', '5f-j).', '5f, g).', '5i).', '5h).', '5j).']","Fig. 5 Comparative pulmonary histologic lesions in AGMs infected with SARS-CoV-2. Representative tissues of AGM from 5 dpi ( - & ) and 34 dpi ( - ). SARS-CoV-2 nave tissues from an AGM ( - ). H&E staining at low magnification (20x) ( , , & ) and higher magnification (40x) ( , , , and inset) of pulmonary alveolar septae and alveolar spaces. Moderate neutrophilic bronchiolitis and alveolitis and mild interstitial pneumonia with congestion ( & ) Moderate lymphohistocytic interstitial pneumonia with congestion, mild alveolar wall fibrosis ( & ) and moderate perivascular lymphocytic cuffs ( inset). No significant lesions ( & ). IHC for anti-fibrin antigen (red) ( , & ). Alveolar spaces are partially to completely flooded with fibrin ( ) Minimal intravascular fibrin labeling ( ) and no significant fibrin immunolabeling ( ). Trichrome special stain for collagen (blue) ( , & ). Minimal to mild alveolar wall collagen deposition ( ) moderate alveolar wall collagen deposition ( ) and minimal collagen staining of alveolar wall basement membranes ( ). IHC labeling for anti-SARS-CoV2 antigen (red) ( , & ). IHC positive type I pneumoncytes (black arrows) and type II pneumoncytes (white arrow) localized with alveolar inflammation ( ), No immonolabeling ( ) and IHC positive labeling of respiratory epithelium of the bronchus ( ). Images captured at 20x ( , , , , & ) and 40x ( , , , & )",yes
PMC10403711,Figure_6,oa_package/6e/ae/PMC10403711.tar.gz,"['Microscopic image of xanthogranulomatous cholecystitis with numerous foamy histiocytes in the gallbladder wall.', 'DiscussionXGC is a form of chronic cholecystitis that commonly presents with symptoms of abdominal pain, nausea, vomiting, weight loss, and anorexia [8,9].']",Fig. 6 Microscopic image of xanthogranulomatous cholecystitis with numerous foamy histiocytes in the gallbladder wall.,yes
PMC10423758,Figure_1,oa_package/5f/86/PMC10423758.tar.gz,[],"FIGURE 1 Ultrasonography of succinate dehydrogenasedeficient renal cell carcinoma. (A) Grayscale ultrasound showed that the cysticsolid mass was oval, welldefined, and approximately 41mm40mm in size. (B) Color Doppler flow imaging showed that the solid component of the mass was isoechoic and approximately 16mm9mm in size without any blood flow signals.",yes
PMC10623084,Figure_2,oa_package/a7/07/PMC10623084.tar.gz,"['The results demonstrated that non-tumor tissues had little EFNA3 expression and BLCA tissues had markedly high expression (A-I).', '.']","Figure 2. Representative images of EFNA3 staining. (A-C) High level of EFNA3 expression in BLCA. (D-F) Immunohistochemical staining of EFNA3 in normal bladder tissue. (G-I) Low level of EFNA3 expression in BLCA. Magnification: original magnification, 40 (left column); 200 (middle column), and 400 (right column). BLCA, bladder urothelial carcinoma; EFNA3, ephrin A3.",yes
PMC10314239,Figure_2,oa_package/07/6d/PMC10314239.tar.gz,"['Gray-scale ultrasound-selected images of the transplanted kidney and upper abdomen show an increased parenchymal echogenicity of the kidney (yellow arrow), with renal peri pelvicalyceal cystic structures and hyperechoic wall/septae (orange arrows).']","Figure 2 Gray-scale ultrasound-selected images of the transplanted kidney and upper abdomen show an increased parenchymal echogenicity of the kidney (yellow arrow), with renal peri pelvicalyceal cystic structures and hyperechoic wall/septae (orange arrows). There is a perinephric fluid collection with septae (blue arrow) along with minimal subhepatic free fluid (green arrow).",yes
PMC9733332,Figure_3,oa_package/60/85/PMC9733332.tar.gz,"[' 3a).', '3b).', 'KPNB1-mediated reduction of TDP-CTF and TDP-43 aggregates depends on its FG-Nup interaction domain.', '001, n = 50 51 cells per group)KPNB1 constructs with the greatest effect on TDP-CTF aggregation are also the constructs that bind to FG-Nups; this raised the question whether the proposed chaperone activity of KPNB1 depends on its interaction with FG-Nups.', '3c).', '3d,e).', '3d).', '3f,h and Supplementary ', '3f-i).', '3f-i).', '3), we hypothesized that either FG-Nups or Ran recruit KPNB1 to TDP-43 aggregates.', '3).', '3b).', '3c-i and ', '3f-i).']","Fig. 3 KPNB1-mediated reduction of TDP-CTF and TDP-43 aggregates depends on its FG-Nup interaction domain. Schematic domain structure of KPNB1. KPNB1 is comprised of 19 HEAT repeats (H119). Ran and importin- interact with H18 and H819, respectively. FG-Nups bind to two regions of KPNB1, H57 and H1416. Lysates from HEK293T cells expressing GFP, GFP-KPNB1 full-length (FL), N-terminal fragments of KPNB1 (H19H1) or the C-terminal fragment of KPNB1 (H1019) were subjected to immunoprecipitation with GFP-Trap magnetic beads. Whole cell lysates (input) and immunoprecipitates (IP) were subjected to western blot analysis using indicated antibodies. KPNB1 H18, the smallest fully active KPNB1 fragment in reducing TDP-43 aggregation, strongly interacts with FG-Nups and Ran, and weakly with importin-1 in comparison with full-length KPNB1. (Top) Schematic domain structure of KPNB1 H18 harboring four missense mutations (I178A, F217A, Y255A, I263R) in the nucleoporin-interacting site (NIS). (Bottom) Lysates from HEK293T cells expressing GFP, GFP-KPNB1 H18 or H18 were subjected to immunoprecipitation with GFP-Trap magnetic beads. Whole cell lysates (input) and immunoprecipitates (IP) were subjected to western blot analysis using indicated antibodies. KPNB1 H18 shows strongly reduced interaction with FG-Nups. Immunofluorescence (IF) of SH-SY5Y cells co-expressing mCherry or mCherry-TDP-CTF with GFP-KPNB1 H18 or H18 . KPNB1 H18 does not reduce cytoplasmic TDP-CTF aggregates. Hoechst staining was used to outline nuclei. Scale bar: 5m. Western blot analysis and quantification of insoluble mCherry-TDP-CTF in SH-SY5Y cells expressing GFP, GFP-KPNB1 H18 or H18 . KPNB1 H18 strongly decreased insoluble TDP-CTF levels, whereas KPNB1 H18 did not. -tubulin was used as a loading control. Statistical analysis was performed using one-way ANOVA and Bonferronis post hoc test (** <0.01, *** <0.001, =4). IF of HEK293T cells co-expressing GFP-(GR) with an empty plasmid (ctrl), FLAG-tagged KPNB1 H18 or H18 and stained for endogenous TDP-43. Unlike KPNB1 H18 , KPNB1 H18 prevents the sequestration of endogenous TDP-43 in cytoplasmic GR aggregates. Arrowheads point to cytoplasmic GR aggregates. Hoechst staining was used to outline nuclei. Scale bar: 5m. Quantification of the percentage of cells with cytoplasmic GR aggregates positive for endogenous TDP-43. KPNB1 H18 significantly reduced the number of TDP-43-positive GR aggregates, while KPNB1 H18 had no effect. Statistical analysis was performed using one-way ANOVA and Bonferronis post hoc test (three independent experiments; * <0.05, *** <0.001, =5052 cells per group). IF of HEK293T cells co-expressing GFP-(GR) with an empty plasmid (ctrl), FLAG-tagged KPNB1 H18 or H18 and stained for endogenous Nup62. Unlike KPNB1 H18 , KPNB1 H18 prevents the sequestration of endogenous Nup62 in cytoplasmic GR aggregates. Arrowheads point to cytoplasmic GR aggregates. Hoechst staining was used to outline nuclei. Scale bar: 5m. Quantification of the percentage of cells with cytoplasmic GR aggregates positive for endogenous Nup62. KPNB1 H18 significantly reduced the number of Nup62-positive GR aggregates, while KPNB1 H18 had no effect. Statistical analysis was performed using one-way ANOVA and Bonferronis post hoc test (three independent experiments; *** <0.001, =5051 cells per group)",yes
PMC7648173,Figure_6,oa_package/b0/84/PMC7648173.tar.gz,"['Twenty-four-hours (24 h)- post-LPS treatment or nutrient deprivation of A549 cells, we analyzed Galuminox uptake by live cell fluorescence imaging ().', 'Cellular accumulation of Galuminox either in serum and glucose deprived- or LPS-treated treated A549 cells either in presence or absence of Carvedilol (CAR),Dexrazoxane(DEX), and MitoTEMPO (MTT): Images were acquired using a 20 objective (all panels represent same magnification) in live human adenocarcinoma alveolar Immediately after recanalization, remarkably high pressures of 90 mmHg in the lateral compartment and 83 mmHg in the anterior compartment were confirmed.', 'Therefore, fasciotomy was performed in the lateral, anterior, posterior, and deep compartments, and satisfactory peripheral blood flow was achieved (c).']","Fig. 2. A 74-year-old woman with popliteal artery occlusion due to iatrogenic injury (Patient 2). (a) Left lower extremity angiogram showing focal thrombotic occlusion (arrow) and dissecting pseudoaneurysm (arrowhead) of the popliteal artery. (b) Despite thrombectomy, the intimal flap (arrow), dissecting pseudoaneurysm, and vasospasm of the distal arteries (arrowhead) remained. (c) Follow-up angiogram 1 week after stent placement showed satisfactory peripheral blood flow and disappearance of the pseudoaneursym (arrow: bare stent).",yes
PMC4516503,Figure_2,oa_package/85/13/PMC4516503.tar.gz,[],"Figure 2 qRT-PCR of genes specific for the classical, the alternative activated type of macrophages and deactivating targets 5 days after myocardial infarction. All fold changes (FC) are expressed relative to mRNA abundance in myocardial infarction (MI), which was set to 1. Tumour necrosis factor (TNF) (A), (IL6) (B) and interleukin 1 (IL1) (C) are all strongly up-regulated in macrophages compared with myocardial infarction, BZ, MonoB and LV. This indicates a myocardial-specific regulation predominantly in macrophages. (ARG1) (D), (ARG2) (E) and (CHI3L3, also designated YM1) (F) are mainly transcribed by macrophages compared with MI, BZ and LV. In connection with ARG2 as well as CHI3L3, a moderate transcription was also detected in monocytes isolated from blood compared with MI, BZ and LV, which indicates an incomplete cardiac-specific expression. (SOCS3) (G), (IL1rn) (H) and (IL10) (I), which are all known to suppress the inflammatory response, are predominantly transcribed by macrophages. Note that especially IL10 shows a very strong up-regulation in macrophages compared with controls. (FC, fold change; LV, left ventricle; MonoB, monocytes isolated from blood; BZ, border zone of infarcted area; MI, entire infarcted area; MAC, macrophages isolated from infarcted area; each = 6).",yes
PMC6204045,Figure_5,oa_package/68/de/PMC6204045.tar.gz,"[' 5, -Syn pffs induce IL-1 expression in WT microglia which is increased by about 65% in G2019S KI cells.', ' 5).', 'LRRK2G2019S KI primary microglia exhibit increased level of pro-inflammatory IL-1 after -Syn pffs priming.', '05)DiscussionAccumulating evidence indicates a functional interaction between LRRK2 and PKA, although the precise molecular mechanisms of this cross-talk still need to be elucidated [31].']","Fig. 5 LRRK2 primary microglia exhibit increased level of pro-inflammatory IL-1 after -Syn pffs priming. LRRK2 WT and G2019S KI microglia lysates treated with 25M -Syn pffs or PBS as control (CTR) were subjected to immunoblotting using P-LRRK2, total LRRK2, IL-1, and GAPDH antibodies. Quantification of IL-1 is normalized for GAPDH. Data are representative of three independent experiments (bars represent the meanSEM; one-sample test; * <0.05)",yes
PMC4585288,Figure_8,oa_package/b1/63/PMC4585288.tar.gz,['Identification of proteins that are citrullinated in response to CCI.'],"Figure 8 . Extracts of control (C) and injured (I) cerebral cortex were fractionated by fluid-phase isoelectric focusing into defined pH ranges (shown at top) and then further resolved according to molecular weight using one-dimensional gel electrophoresis. Proteins were then transferred to nitrocellulose membranes and probed for protein-bound citrulline (see ) (right panel, Western blot). Gels run in parallel were visualized with Coomassie (left panel). Sixteen features showing increased citrullination in response to CCI (black numbered boxes, right panel) were mapped to corresponding Coomassie features (red numbered boxes, left panel) and identified by peptide mass finger printing and tandem mass spectrometry. Proteins identified are listed in the lower panel. Analysis of the mass spectra data set with a variable modification search for the citrullination of arginine residues revealed several proteins with sites of citrullination on arginine residues with Mascot score corresponding to <0.05 (Cit Pep). Several of these proteins (Prev Report) are also citrullinated in other pathologies, including ALDOA, ALDOC, GFAP, PRDX1, COF1, ENOA, ENOG, MBP, and tubulin (beta) in prion disease ( , ); GFAP and MBP in Alzheimers disease and multiple sclerosis ( ); ENOA in rheumatoid arthritis ( ); and GRP78 in Type I diabetes ( ). Images are representative of six independent experiments. A total of =4 CCI and =4 control animals were examined.",yes
PMC6915767,Figure_6,oa_package/06/eb/PMC6915767.tar.gz,[],"Figure5 Tau Is Found in Pre- and Postsynapses in Human AD Brain (AF) Array tomography was used in human AD and control postmortem brain tissue to stain A (white), Tau13 (yellow), PSD95 (magenta), and synaptophysin (cyan). Tau13 stains neuropil threads (A, arrows). Examining individual synapses revealed that A was present in 8.0% of presynaptic terminals (B, arrowheads) and 10.4% of postsynaptic densities (B, arrows) near plaques in AD cases (B and E). Tau13 staining was observed in 0.23% of presynaptic terminals (C, arrowheads, quantified in F), and 0.32% of postsynaptic terminals (D, arrows, quantified in F). (GI) Misfolded tau labeled with Alz50 (yellow) was observed in neuropil threads (G, arrows) and in presynapses (H, arrowheads) and postsynapses (I, arrows). (J) Tau phosphorylated at serine 202 (labeled with CP13) and misfolded (residues 515 near 312322, labeled with MC1) was observed in PSDs. Images in (A) and (G) and large panels in (J) are maximum intensity projections of 10 serial sections. Scale bar represents 10m in (A), (G), and (J). (B)(D), (H), (I), and insets in (J) show three-dimensional reconstructions of a 2 2 micron region of interest in 5 consecutive serial 70-nm sections. Data shown are median and interquartile ranges. Each dot represents the median of a single human subject (subject is the biological replicate/experimental unit, n= 6 per group). See also .",yes
PMC7461256,Figure_4,oa_package/05/43/PMC7461256.tar.gz,"['4a / b) compared to control.', '4c / d).', 'Immunohistochemical analysis of cell proliferation (a, b) and cell death (c, d).', 'AEC detection, bar 50 m (inset bar 25 m), inset as negative control without first antibodySertoli cell function and blood-testis barrier formation was analyzed by ZO-1, claudin 11 and connexin (CX) 43 immunohistochemistry in control (']","Fig. 4 Immunohistochemical analysis of cell proliferation ( , ) and cell death ( , ). Pictures and show intact spermatogenesis (NSP) as control, pictures and show spermatogonial arrest (patients left testis). , Proliferation was tested by PCNA immunostaining and revealed intensely stained spermatogonia (black arrows) and primary spermatocytes (black arrowheads). In the spermatogonial arrest tubules, PCNA staining was detected in more spermatogonia compared to NSP. , TUNEL staining revealed apoptotic germ cells in NSP (red arrows) and to a higher extend in tubules with abundant spermatogonia. AEC detection, bar 50m (inset bar 25m), inset as negative control without first antibody",yes
PMC9316148,Figure_3,oa_package/fe/e3/PMC9316148.tar.gz,"['After achieving an effective knock-down at the protein and RNA levels (A), we evaluated the effects of ERK5 abrogated expression in terms of cell growth (B), showing how the lack of ERK5 promoted a marked delay in cell growth, especially at later time points.', 'Interestingly, cells with ERK5 abrogated expression exhibited a marked decrease in the number of colonies (C), suggesting that ERK5 could have some effect not only in the clonogenic growth of our sarcoma-derived cells but also in the attachment ability, as it has been described in other experimental models [37,38].', 'Therefore, adhesion assays in fibronectin-coated wells were performed, but no significant differences were observed (D).', 'As it is depicted (E), the lack of ERK5 promoted a marked delay in the in vivo growth.', '9), tumors derived from the interfered lines were 5 7 times lower (E).', 'In fact, analysis of tumors showed a marked recovery of ERK5 expression in tumors originating from cell lines with abrogated ERK5 expression, as well as no morphological differences between both groups (F,G).', 'ERK5 modulates in vitro and in vivo growth of 3MC-C1 cell line.']","Figure 3 ( ) 3MC-C1 cells were infected with lentiviruses carrying PLKO.1 empty vector (E.V.) or the PLKO.1-shRNA ERK5-1 vector (shERK5-1). Interference was evaluated by RT-qPCR using -2-microglobulin ) as an endogenous control ( panel). E.V. cells were considered as 1. panel shows a representative image of the interference by western blot using vinculin as a loading control. ( ) For growth curves, 3 10 E.V. or shERK51 3MC-C1 cells were seeded in 100 mm plates. Every 3 days, cells were counted and replated in the same manner up to day 9. Graphic shows the cumulative cell number from a representative experiment out of 3 with nearly identical results in different pools of infections. ( ) Upper panel: Relative number of colonies obtained in clonogenic assays of E.V. and shERK5-1 3MC-C1 cells. Lower panel: Representative image of a colony formation assay from both cell lines. ( ) Upper panel: Relative adhesion of E.V. or shERK5-1 3MC-C1 cells was assessed by crystal violet staining at the indicated time points. Lower panel: Representative image of adhesion assays at 7.5 and 60 min. ( ) Nude mice (n = 4) were inoculated with 5 10 cells of each cell line derived from 3MC-C1, and tumorigenesis was analyzed at indicated times. Graphics represent the mean SD. The experiment was performed by using another pool of infection with nearly identical results. ( ) Western blot analysis of the expression level of ERK5 in tumors recovered from 3MC-C1 E.V. and shERK5-1. Vinculin was used as a loading control. ( ) Representative images of the histological study of tumors obtained from 3MC-C1 derived cell lines. Pictures are shown at a 20 magnification. The graphs/histograms represent the mean SD of 3 independent experiments performed in triplicate cultures with different pools of infections, if not otherwise indicated. Original blots could be found in . * < 0.05; ** < 0.01; and *** < 0.001.",yes
PMC3787765,Figure_3,oa_package/fb/bf/PMC3787765.tar.gz,[],"Fig.3 SAA and SAP were present in amyloid deposits of AKU cartilage and synovia and both co-localized with melanin. A) SAA and SAP deposition in AKU cartilage and synovial specimens was detected by dual immunofluorescence technique. AKU cartilage and synovia showed high levels of SAA deposit superimposing SAP deposits. Positive staining for SAA and SAP was particularly intense in correspondence of ochronotic shards. Bar: 75m; B) AKU cartilage sections were dual-stained using antibodies specific for SAA SAP and compared to melanin fluorescence, resulting in a perfect co-localization of amyloid deposits and pigmented areas. Cartilage specimen was from Patient 6 and synovia specimen was from Patient 1. DIC: differential interference contrast. Bar: 150m. Representative images from a triplicate set are shown.",yes
PMC11707698,Figure_1,oa_package/14/a5/PMC11707698.tar.gz,"['Co-expression with Myc enhances the yield of TOMWe employed Drosophila as an in vivo expression host for transgenic expression of the Drosophila Tom40 subunit fused to a C-terminal FLAG-HA epitope tag ( ).', 'The bipartite UAS GAL4 system [ (a)] (Bischof et al.', 'Overall, this strategy resulted in an enhancement of the yield of TOM for structural studies [ (d)].', 'Isolation and reconstitution of Drosophila TOMBlue native polyacrylamide gel electrophoresis (BN-PAGE) analysis of detergent-solubilized fly head membranes [ (b)] ascertained that tagged Tom40 predominantly assembled into an 480 kDa band [', '1 (c)] corresponding to the TOM complex (Dekker et al.', 'Solubilization with digitonin destabilized the complex, resulting in a dissociated lower-order species that was not observed when the membranes were solubilized with an n-tetradecyl- -d-maltopyranoside cholesterol hemisuccinate combination [ (c)].', ' 1 (d)].', 'This may suggest that Tom40 or contaminating VDAC [ (d)] (K nkele et al.', 'Isolation of a Drosophila TOM complex.', 'Structure of the core TOM complex of D.']","Figure 1 Isolation of a TOM complex. ( ) Schematic representation of the UASGAL4 protein-expression system in . The promoter (GMR) drives ectopic expression of Tom40 in fly retina via the transcription factor GAL4 and the GAL4 response element UAS. This image was created with BioRender.com. ( ) Workflow of protein purification. Fly heads were separated using a metal sieve stack. Fly heads collected in the 500m compartment were homogenized and membranes were prepared by ultracentrifugation of the lysate. Membranes were detergent-solubilized and the protein was affinity-purified using anti-FLAG resin. ( ) Comparative BN-PAGE Western blot (anti-HA) analysis of eye membranes, expressing Tom40 (co-expressed with Myc), solubilized with either digitonin or -tetradecyl-- -maltopyranoside (TetraDM)cholesterol hemisuccinate (CHS). Additional bands migrating close to the 146 and 720kDa markers indicate dissociated complexes and super-complexes, respectively. ( ) Silver-stained SDSPAGE gel analysis of immuno-affinity-purified TOM complex reconstituted into non-ionic amphipol. Protein identities of prominent gel bands detected by tryptic digest mass spectrometry are labeled with asterisks.",yes
PMC2270292,Figure_2,oa_package/56/95/PMC2270292.tar.gz,"['In order to map Winnie, we outcrossed an affected G4 to NODk mice, and intercrossed their F1 progeny; 46/172 (27 ) F2 mice had the diarrheal phenotype, consistent with a fully penetrant recessive trait (A).', '5 Mb interval on Chromosome 7 encoding 198 transcripts including the Muc2 and Muc6 mucin genes (B).', 'gov) resulting in substitution of cysteine with tyrosine in the D3 domain at the N terminus of Muc2 (C).', 'g001""/>Generation and Characterization of Mice with Muc2 Mutations(A) Dendrogram showing genesis of the Winnie mutation; filled symbols indicate the diarrhoea phenotype.', 'We identified a second strain, Eeyore, from a different G0 founder, with a similar phenotype also inherited as a fully penetrant recessive trait (D).', 'In a Win/Win Eey/ complementation test, eight of 22 offspring exhibited the clinical and histopathological phenotype (D).', 'AJ511873) resulting in a serine to proline substitution in the C-terminal D4 domain (C).', 'Therefore, to ascertain whether the Winnie mutation affects biosynthesis of Muc2, we cloned partial cDNAs encoding the Winnie and wild-type Muc2 N-terminal D3 oligomerisation domain (rMuc2-D3, C) and expressed these as recombinant proteins in MKN45 gastric cancer cells which produce the MUC5AC mucin (but not MUC2) and are therefore likely to express mucin-specific chaperones.']","Figure 2 Generation and Characterization of Mice with Mutations (A) Dendrogram showing genesis of the mutation; filled symbols indicate the diarrhoea phenotype. (B) Microsatellite genotype on Chromosome 7 of 17 affected F2 mice. (C) Domain organization of the Muc2 protein showing the N- and C-terminal vWF D-domains (D1D4), the C-terminal B, C, and CK domains, and the two central glycosylated tandem repeat domains (VNTR). The sites of the and mutations are shown, together with the region cloned to express the rMuc2-D3 recombinant protein. (D) Results of complementation cross of and . PAS-stained sections of representative distal large intestinal histological phenotypes demonstrate noncomplementation (preservation of the / histological phenotype in / ).",yes
PMC5494403,Figure_3,oa_package/b9/b9/PMC5494403.tar.gz,"['\nMRI scan of the brain, axial view (A) and coronal view T2WI (B) showing isointense sellar, suprasellar lesion.']","Figure 3 scan of the brain, axial view (A) and coronal view T2 (B) showing isointense sellar, suprasellar lesion. (C&D) after intravenous injection of gadolinium, showing enhancement of the mass lesion.",yes
PMC11373135,Figure_1,oa_package/75/ff/PMC11373135.tar.gz,"['3Diagnostic assessmentGrossly, only Bov-fet-1 had severe lung consolidation following palpation of the entire lobes (s 1A,B).', 'Gross findings of Nocardia farcinica abortion in a bovine fetus.']",Figure 1 Gross findings of abortion in a bovine fetus. Bov-fet-1. Lungs. Severely consolidated lungs following palpation of the entire lobes (black arrows). Bov-fet-1. Lungs. Cut section of severely consolidated lungs.,yes
PMC6212693,Figure_1,oa_package/4f/b8/PMC6212693.tar.gz,"['A well demarcated mass with multiple calcifications had broad dural base suggesting extra-axial tumor ().', '1PerryALouisDNScheithauerBWBudkaHvon DeimlingAMeningiomasLouisDNOhgakiHWiestlerODCaveneeWKWorld Health Organization classification of tumours of the central nervous systemLyonIARC Press20071641722HuangJPeterssonFIntracerebral metaplastic meningioma with prominent ossification and extensive calcificationRare Tumors20113e20217693193TangHSunHChenHClinicopathological analysis of metaplastic meningioma: report of 15 cases in Huashan HospitalChin J Cancer Res201325112118233723494MatyjaENaganskaEZabekMJagielskiJMeningioma with the unique coexistence of secretory and lipomatous components: a case report with immunohistochemical and ultrastructural studyClin Neuropathol200524257261163208195JohnsonMDStevensonCBThompsonRCAtkinsonJBoyerPDecember 2006: 31-year-old woman with hemiparesisBrain Pathol200717255257173889586MajumdarKMandalSThakkarRSaranRKSrivastavaAKMeningeal osteochondroma simulating meningioma with metaplastic change: a rare golf-ball-like lesion of non-meningothelial mesenchymal originBrain Tumor Pathol201431626723456087Brain CT shows about 6.']","Fig. 1 Brain CT shows about 6.74.5 cm sized, low-density, broad dural based, and inhomogeneous enhancing mass with multiple calcifications in the left parietal region which is suggesting extra-axial tumor. Definite subdural lesion is not seen on the brain CT.",yes
PMC4442895,Figure_2,oa_package/81/8f/PMC4442895.tar.gz,['A coronal section of an unenhanced CT taken for right ureteric colic.'],"Figure 2 A coronal section of an unenhanced CT taken for right ureteric colic. No urolithiasis was identified. However, a dilated tubular blind-ended bowel loop (arrow) arising from the caecum (arrowhead) with surrounding fat stranding was apparent, consistent with acute appendicitis.",yes
PMC7555595,Figure_4,oa_package/cd/5b/PMC7555595.tar.gz,"['Histologically, leiomyosarcoma typically shows fascicles of spindle-shaped tumor cells with blunt-ended nuclei and moderate to abundant, brightly eosinophilic fibrillary cytoplasm ().', 'Leiomyosarcoma.']","Figure 4 Leiomyosarcoma. ( ) The tumor cells have cigar-shaped, blunt-ended nuclei with brightly eosinophilic cytoplasm and a fascicular pattern. Mitoses are present. ( ) The tumor cells are diffusely positive for SMA (left) and desmin (right) (H&E stain, original magnification 20 ; SMA and desmin immunostain, original magnification 200 ).",yes
PMC8293917,Figure_1,oa_package/6d/38/PMC8293917.tar.gz,"['Further, p- -syn signal can be observed in extracellular space as well and in CA1 stratum oriens (CA1so) and stratum radiatum (CA1sr) (E).', 'In granule cell layer (GCL) of DG p- -syn is expressed to a lesser extent compared to CA1sp region but in a similar fashion (F).', 'Cells with p- -syn accumulation that follow the same pattern of expression as in CA1sp and GCL can be observed in PL as well (F).', 'While nestin expression could be associated with individual neurons in WT mice, net-like pattern of expression was observed in Thy1-aSyn animals (Supplementary ).', 'Of note, this does not directly correlate with the apparent site and subregion specific p- -syn pathology observed in the hippocampus ().', '691560/full#supplementary-materialSupplementary Nestin expression and Ki-67 positive cell density in the hippocampal dentate gyrus of 6 months old WT and Thy1-aSyn mice.']","FIGURE 1 Expression of the p--syn in the hippocampus of the 6 and 16-month-old WT and Thy1-aSyn mice. Double-immunofluorescence for p--syn (green) and NeuN (orange) and DAPI staining (blue) and merged images of 6 and 16 months-old wildtype [WT; ], and 6 and 16 months-old Thy1-aSyn [asyn; ] mice. Cornu ammonis (CA1, CA2, and CA3), dentate gyrus (DG) regions. Scale bars, 200 m. High magnification Apotome images (63, oil immersion), double-immunofluorescence for p--syn (green) and NeuN (orange) and DAPI staining (blue) and merged images of Thy1-aSyn mice . CA1so, CA1 stratum oriens; CA1sr, CA1 stratum radiatum; CA1sp, CA1 pyramidal layer , GCL, DG granule cell layer; DGmo, DG molecular layer; PL, DG polymorph year . Arrows indicate NeuN positive cells and p--syn accumulations. Scale bar 50 m.",yes
PMC4124241,Figure_2,oa_package/73/2f/PMC4124241.tar.gz,"['001""/>Nasal mucosa: PLUNC positive (++) IHC 40x.']",Figure 2 Nasal mucosa: PLUNC positive (++) IHC 40x. ++ PLUNC antibodies positivity in epithelium () and in stroma ().,yes
PMC9724508,Figure_3,oa_package/4e/e0/PMC9724508.tar.gz,"['\n18F-fluorodeoxyglucose positron emission tomography and computed tomography revealed that the right side of brachial plexus nodular appearance with increased 18F-fluorodeoxyglucose metabolism, a SUVmax of 13.']","Figure 3 F-fluorodeoxyglucose positron emission tomography and computed tomography revealed that the right side of brachial plexus nodular appearance with increased F-fluorodeoxyglucose metabolism, a SUVmax of 13.7.",yes
PMC3227004,Figure_6,oa_package/5c/88/PMC3227004.tar.gz,"['Vaccinated APPSw/NOS2 / mice showed significant reductions in TNF and IL-6 mRNA compared with control vaccinated APPSw/NOS2 / mice, yet TNF and IL-6 mRNAs were still significantly increased compared with WT and NOS2 / mice (A).', 'Interestingly, alternative inflammatory genes YM1 and AG1 were significantly reduced, not only compared with control vaccinated APPSw/NOS2 / but also compared with WT and NOS2 / mice (B).', 'In addition, TGF was reduced approximately to WT levels following vaccination (B).', 'Inflammatory genes Mrc1 and CD163, known to be restricted to the cerebrovasculature, decreased compared with both control vaccinated APPSw/NOS2 / mice, and WT mice (C).', 'Active immunization alters the immune profile in APPSw/NOS2 / miceMice were immunized with either A -(1 42) or KLH at 12 months of age as described in the Materials and methods section and compared with age-matched NOS2 / mice immunized in a similar fashion.']","Figure 6 Active immunization alters the immune profile in APPSw/NOS2 mice Mice were immunized with either A-(142) or KLH at 12 months of age as described in the Materials and methods section and compared with age-matched NOS2 mice immunized in a similar fashion. ( , ) Results represent the meansS.E.M. fold-change in mRNA levels for selected classical activation genes ( ) and for selected alternative activation and acquired deactivation genes ( ) in APPSw/NOS2 mice. ( ) mRNA for alternative activation genes ( ) associated with the cerebrovasculature ( =57 mice/group in all cases). ** <0.01, *** <0.001 compared with control mice. <0.05, <0.01 compared with the A42 immunized condition in each case.",yes
PMC11490282,Figure_2,oa_package/0c/9d/PMC11490282.tar.gz,[],Figure 2 Ulcerative lower leg lesion,yes
PMC4573408,Figure_2,oa_package/af/4d/PMC4573408.tar.gz,"['A subsequent computed tomography (CT) scan then confirmed a densely calcified mass within the right nasal cavity with mucosal thickening, closely related to the turbinate, demonstrating expansion of the lateral nasal wall, .', 'CT images confirming the rhinolith.']",Fig. 2 CT images confirming the rhinolith.,yes
PMC7307625,Figure_9,oa_package/97/6a/PMC7307625.tar.gz,"[' in apical 5-chamber view shows an isoechoic round mass jutting into the LA attached by a small pedunculated trait to the basal wall of the LA.', '[49]Transthoracic echocardiography apical five-chamber view showing a myxoma into the left atrium; white arrow: Myxoma; RA = Right atrium; LA = Left atrium0Transthoracic echocardiography subcostal view showing a myxoma into the left atrium attached to the basal part of the interatrial septum; white arrow: Myxoma; white head arrow: Interatrial septum; RA = Right atrium; LA = Left atriums 11 and 12 show interesting 3D TEE and TOE pictures of a fibroelastoma attached to the aortic left coronary aortic cusp; here, the isoechoic dense nature of the rounded pedunculated mass can be clearly identified, attached to the left coronary aortic cusp, and can be measured and accurately evaluated in its shape using 3D TTE and TOE scans.']",Figure 9 Transthoracic echocardiography apical five-chamber view showing a myxoma into the left atrium; white arrow: Myxoma; RA = Right atrium; LA = Left atrium,yes
PMC9740753,Figure_3,oa_package/a9/7a/PMC9740753.tar.gz,"['From a therapeutic point of view (), considering that IL-33 induces the production of pro-inflammatory cytokines from cells that express ST2 cells and hematopoietic cells including ILC2s, mast cells, Th2 cells, eosinophils, basophils and dendritic cells, the potential use of IL-33-blocking agents may be a novel therapeutic strategy to treat allergic diseases and some conditions characterized by this inflammatory profile [36,79].', 'Possible therapeutic strategies.']",Figure 3 Possible therapeutic strategies.,yes
PMC3843339,Figure_14,oa_package/a6/38/PMC3843339.tar.gz,[],"Figure 14 (A) Morphea presenting as multiple, superficial, ill-defined erythematous to violaceous lesions. (B) HRUS shows diffusely thickened, slightly hypoechoic dermis in the area of lesions compared to contralateral normal skin",yes
PMC7605829,Figure_6,oa_package/be/5b/PMC7605829.tar.gz,['pylori microorganisms ( ) becomes very difficult due to the dirty background of the stain in comparison to s 1 and 4 which represent the same case (PAS-AB stain 400 )Gastritis without H.'],"Figure 6 Gastritis without infection: The background of the stain creates a delusion about the presence of fake microorganisms (). This case had been diagnosed as positive for the infection on PAS-AB, but when it compared with both H&E and Giemsa stains, it become clearly negative (PAS-AB stain 400)",yes
PMC6235647,Figure_3,oa_package/3d/7e/PMC6235647.tar.gz,['MRI with apparent diffusion coefficient confirming infarct in the area supplied by the AOP.'],"Figure 3 MRI with apparent diffusion coefficient confirming infarct in the area supplied by the AOP. MRI, magnetic resonance imaging; AOP, artery of Percheron.",yes
PMC5662726,Figure_3,oa_package/41/50/PMC5662726.tar.gz,"[' 3A and C) and baseline as well as LPS-stimulated TNF- mRNA levels in Huh7 cells (', ' 3A).', ' 3C and D).', ' 3B and E) and markedly decreased proliferation of Huh7 cells upon LPS stimulation (', ' 3E).', ' 3G).', 'Nox4 siRNA and DPI suppressed LPS induced TNF- and PCNA elevation.', '\nDeletion of the Nox4 gene attenuates LPS-induced TNF- expression in primary hepatocytes in miceTo confirm the role of Nox4 in the LPS-induced TNF- elevation in hepatocytes, we next used primary murine hepatocytes isolated from Nox4 wild type (Nox4 WT) and Nox4 knockout (Nox4 KO) mice.']","Figure 3 Nox4 siRNA and DPI suppressed LPS induced TNF- and PCNA elevation. Nox4, TNF- ( ) and PCNA ( ) mRNA levels were analyzed by qRT-PCR in Huh7 cells transfected with control or Nox4 siRNA for 48 hrs, and either treated with LPS or PBS for an additional 3 hrs. ( ) Nox4, TNF- and PCNA protein levels were determined by western blotting in Huh7 cells treated with controlor Nox4 siRNA for 48 hrs, and either treated with LPS or PBS for an additional 3 hrs. Actin was used as a control for protein loading. ( , ) Huh7 cells treated with controland Nox4 siRNA for 48 hrs,and either untreated or treated with LPS for an additional 3 hrs were analyzed for the cellular level of TNF- ( ), and PCNA ( ) protein by confocal microscopy. Confocal microscopic fields are shown in ( , ), a representative picture of three independent experiments. ( ) Huh7 cells transfected with control and Nox4 siRNA for 24 hrs, and either treated with LPS or PBS for an additional 24 hrs. Then, cell numbers were measured by cell proliferation assay. ( )TNF- and PCNA mRNA levels were analyzed by qRT-PCR in Huh7 cells either untreated or pretreated with DPI (10M) for 30minutes before 3 hrs of LPS stimulation. indicates statistically significant difference from the corresponding controls (p<0.05). indicates statistically significant difference from control siRNA LPS () or DPI (0) LPS () (p<0.05). Lines with p values also indicate statistically significance (p<0.05) between the groups.",yes
PMC2615356,Figure_1,oa_package/80/da/PMC2615356.tar.gz,"['Eighteen cases of PASH showed the presence of a well-circumscribed solid mass, with hypoechoic texture with or without heterogeneity, and parallel orientation ().', 'Some observations regarding its clinicopathologic spectrumCancer19896311541160291731818SngKKTanSMMancerJFTayKHThe contrasting presentation and management of pseudoangiomatous stromal hyperplasia of the breastSingapore Med J200849e82e851836299419PruthiSReynoldsCJohnsonREGisvoldJJTamoxifen in the management of pseudoangiomatous stromal hyperplasiaBreast J2001743443911843858Transverse sonography of PASH shows an approximate 5 cm sized well-circumscribed homogeneous hypoechoic oval mass in a 30-year-old woman who presented with a palpable mass in the right breast.']","Fig. 1 Transverse sonography of PASH shows an approximate 5 cm sized well-circumscribed homogeneous hypoechoic oval mass in a 30-year-old woman who presented with a palpable mass in the right breast. PASH, pseudoangiomatous stromal hyperplasia.",yes
PMC10285468,Figure_1,oa_package/21/24/PMC10285468.tar.gz,"['Her keloids were unsuccessfully managed with intralesional triamcinolone injections for 10 years ().', 'Keloids.']",Fig 1 Keloids. Patients and failing multiple attempts of intralesional triamcinolone.,yes
PMC6190625,Figure_3,oa_package/b3/6d/PMC6190625.tar.gz,"['Severe disruption: Combined disruption of the EZ and ELM ().', 'Early ERM 3 months with CME.']","Figure 3 Combined disruption of the EZ and ELM. EZM, external limiting membrane; EZ, ellipsoid zone; IS/OS, inner segment/outer segment.",yes
PMC10380847,Figure_1,oa_package/11/9b/PMC10380847.tar.gz,"['ResultsFrom 2003 to 2016, 4090 consecutive patients were included in ASTRAL, of whom 551 patients with MCA strokes, a high-quality CTA and CTP that met the requisite threshold were included in the present study ().', '2071316240337This flow diagram illustrates the selection strategy and inclusion/exclusion criteria used to derive the final study population.']",Figure 1 This flow diagram illustrates the selection strategy and inclusion/exclusion criteria used to derive the final study population.,yes
PMC5214942,Figure_8,oa_package/85/f3/PMC5214942.tar.gz,"['Reg3 and Reg3 perpetuate STAT3 and Akt activation in a forward amplification loop to sustain IEC proliferation and tissue repair, which may contribute to the previously reported heightened susceptibility to colorectal cancer ().', 'org/1999/xlink"" xlink:href=""cmi201635f7""/>Our model depicting the role of the AIM2 inflammasome in maintaining intestinal homeostasis and how loss of Aim2 promotes dysbiosis and colitis.']","Figure 8 Our model depicting the role of the AIM2 inflammasome in maintaining intestinal homeostasis and how loss of promotes dysbiosis and colitis. AIM2 promotes IL-18 production in response to microbial DNA in IECs, which downregulates the expression of and consequently enhances IL-22 activity in the colon. This is required for a balanced expression of antimicrobial peptides, including Reg3 and Reg3, to prevent dysbiosis. In addition, IL-18 is capable of positively regulating Reg3 and Reg3 expression. Conversely, loss of impairs IL-18 production, enhances IL-22BP and consequently impairs IL-22, and Reg3 and Reg3 expression, resulting in dysbiosis and heightened susceptibility to colitis. During colitis, IL-18 production is enhanced, which downregulates IL-22BP expression and amplifies IL-22-mediated STAT3 activation to promote Reg3 and Reg3 production. In turn, Reg3 and Reg3 further promote and sustain STAT3 and Akt activation in a self-amplifying feed-forward loop, which promotes proliferation of crypt cells and intestinal repair. Loss of dysregulates this signaling axis, resulting in improved proliferation and repair during the resolution of colitis, which may eventually lead to colorectal cancer.",yes
PMC11253903,Figure_1,oa_package/62/1b/PMC11253903.tar.gz,"['2cm and was 4 cm above the apex ().', 'Gross view of heart with the cyst in the anterior ventricular wall (scale bar = 5cm).']",Figure 1 Gross view of heart with the cyst in the anterior ventricular wall (scale bar = 5cm).,yes
PMC8878440,Figure_2,oa_package/17/cf/PMC8878440.tar.gz,"['Current Approaches and OutcomesThe advent of three-dimensional electroanatomic mapping systems and advances in ablative techniques have resulted in significant improvement in outcomes () [47,48].', 'Catheter ablation in congenital heart diseases, technological advances for challenging cases.']","Figure 2 Catheter ablation in congenital heart diseases, technological advances for challenging cases.",yes
PMC7498263,Figure_1,oa_package/cf/8b/PMC7498263.tar.gz,"['As might be expected, mice that received the PTX declared the disease earlier than mice that did not (A).', 'Using a standard five-point EAE grading scale, we could separate the EAE mice that did not receive PTX into two distinct cohorts (A): mice with a high clinical score (HIS; 53% of the immunized mice) and mice with a low clinical score (LIS; 47%).', 'Accordingly, we could observe decreased fluoromyelin staining in the spinal cord of EAE mice (B).', 'Indeed, the decrease in fluoromyelin staining was more marked in the HIS group compared to the LIS group, consistent with the higher clinical score (B).', 'Here again, we observed two distinct phenotypes, that is HIS and LIS, with a similar distribution ( figure supplement 1A).', 'This decrease was more marked in the HIS group compared to the LIS group ( figure supplement 1B).', '.', ' figure supplement 1.', 'To further explore the differences between LIS and HIS, we first assessed CD3 immunoreactivity in the spinal cord as it reflects lymphocyte infiltration.', 'CD3 immunostaining in all the mice that received the immunizing peptide was similar (C D).', 'We found, both in the white and grey matters, larger Iba-1-occupied and GFAP-positive areas in the HIS group compared to the LIS group (E G).', 'Based on the presentation of , I assume that the LFB stain was combined with a Cresyl-Violet stain or some similar one.', '10) In I suggest to add information regarding what was stained in panel D and also in .', 'In E the labels are very small in size and hard to read.', 'Based on the presentation of , I assume that the LFB stain was combined with a Cresyl-Violet stain or some similar one.', 'In we did not report LFB stain.', ' figure supplement 1 shows LFB with Cresyl-Violet in the spinal cord and A-C shows LFB and Cresyl-Violet in the cortices.', '10) In I suggest to add information regarding what was stained in panel D and also in .', 'In E the labels are very small in size and hard to read.', 'Regarding D, the information is stated in the figure legend: Representative photomicrographs of CD3 positive cells (lymphocytes) infiltrating the spinal cord ventral white matter.', 'We also modified the size of the labels in E in order to facilitate the reading.']","Figure 1. Clinical and immunohistological characterization of the EAE model with high and low clinical scores. To induce EAE, mice received MOG and complete Freunds adjuvant (CFA) with or without pertussis toxin (PTX). ( ) Clinical score of EAE mice receiving PTX (PTX group) or no PTX and with a high clinical score (HIS) or low clinical score (LIS). ( ) Quantification of fluoromyelin signal in the spinal cord ventral white matter. ( ) Quantification of the CD3 positive area in the ventral white matter of the spinal cord. ( ) Representative photomicrographs of CD3 positive cells (lymphocytes) infiltrating the spinal cord ventral white matter. The scale bar represents 50 m. The quantification of the entire cohort is shown in ( ). ( ) Representative photomicrographs of GFAP positive cells (astrocytes) and Iba-1 positive cells (microglia, monocytes, macrophages) in the spinal cord grey and white matters of mice. The scale bar represents 50 m. ( - )Quantification of GFAP positive area and Iba-1 positive area in ( ) the grey matter and in ( ) the white matter of the spinal cord. The scale bar represents the indicated length. Data are meansem. N=712/group. For A, two-way ANOVA with Sidaks post-hoc test. For B to F, one-way ANOVA with Sidaks post-hoc test. p0.05 p0.01 p0.001 vs CTL, #p0.05 ### p0.001 vs PTX, *p0.05 **p0.01 ***p0.001 vs LIS.",yes
PMC4668954,Figure_1,oa_package/77/26/PMC4668954.tar.gz,"['A peripheral blood smear showed a leukoerythroblastic picture with several blast-like cells with fine chromatin and one or more prominent nucleoli ().', '1182/blood-2003-05-16751290744120GaulardPBelhadjKReyesFGammadelta T-cell lymphomasSemin Hematol2003403233431287667221VegaFMedeirosLJGaulardPHepatosplenic and other gammadelta T-cell lymphomasAm J Clin Pathol20071276869801750998422VoseJArmitageJWeisenburgerDInternational peripheral T-cell and natural killer/T-cell lymphoma study: pathology findings and clinical outcomesJ Clin Oncol200826254124301862600523DommJAThompsonMKutteschJFAcraSFrangoulHAllogeneic bone marrow transplantation for chemotherapy-refractory hepatosplenic gammadelta T-cell lymphoma: case report and review of the literatureJ Pediatr Hematol Oncol20052711607101628289324GassasAKirbyMWeitzmanSNganBAblaODoyleJJHepatosplenic gammadelta T-cell lymphoma in a 10-year-old boy successfully treated with hematopoietic stem cell transplantationAm J Hematol200475211341475538025OtrockZKHatoumHASalemZMLong-term remission in a patient with hepatosplenic gammadelta T cell lymphoma treated with bortezomib and high-dose CHOP-like chemotherapy followed by autologous peripheral stem cell transplantationAnn Hematol20088712102341858757726JaegerGBauerFBrezinschekRBeham-SchmidCMannhalterCNeumeisterPHepatosplenic gammadelta T-cell lymphoma successfully treated with a combination of alemtuzumab and cladribineAnn Oncol20081951025618375525Peripheral blood smear showing a leukoerythroblastic picture with severe thrombocytopenia (Wright stain 400).']","Figure 1 Peripheral blood smear showing a leukoerythroblastic picture with severe thrombocytopenia (Wright stain 400). Inserts showing examples of the circulating lymphoma cells having blastic morphology; large in size with finely dispersed chromatin and prominent nucleoli with irregular nuclear contour (1,000).",yes
PMC5553757,Figure_1,oa_package/2a/62/PMC5553757.tar.gz,"['1a), in corroboration with previous studies showing increased A 42 load in DLB [25 27].', '1b).', 'Bar charts showing concentrations of total A 42 in BA9.', '01\nDemographic and disease variables of participantsDemographic and disease variables of the 61 selected subjects are summarized in Table 1.', '1a) which corroborates earlier findings showing higher A burden in various brain regions of DLB patients [25 27].']","Fig. 1 Bar charts showing concentrations of total A42 in BA9. the complete cohort containing 109 subjects and the 61 subjects (21 Controls, 19 dementia with Lewy Bodies [DLB], 21 Parkinsons disease dementia [PDD]) selected for the current study. Data were presented as meanSEM of total A42 (ng/mg brain protein). Significant difference (Kruskal Wallis ANOVA followed by Dunns tests) * 0.05 or 0.01",yes
PMC7538583,Figure_3,oa_package/09/da/PMC7538583.tar.gz,"[' 3).', 'Significant increases in lung histiocytosis and inflammation were observed at 1 4 16 and 19 psi pressure groups; however, inflammation and histiocytosis was observed to be increased in 1 13 side group but no changes were observed in other groups of 13 psi pressure groups, 14 (8.', 'Mathematical modellingThe R2 values for the 2D models are in Table 1 below, while the area under the curves of the ROC curves for the 3D models (of which the power function versions are shown in ', ' 3).']","Figure 3 Significant increases in lung histiocytosis and inflammation were observed at 1416 and 19 psi pressure groups; however, inflammation and histiocytosis was observed to be increased in 113 side group but no changes were observed in other groups of 13 psi pressure groups, 14 (8.5 and 10 psi) and 30 (8.5 psi) pressure group.",yes
PMC10530233,Figure_12,oa_package/e9/07/PMC10530233.tar.gz,[],Figure 12 The use of 3D transesophageal echocardiography (TEE) in demonstrating the mitral valve from the left atrial perspective: ( ) TEE imaging planes of the mitral valve and ( ) the 3D volume.,yes
PMC9590112,Figure_3,oa_package/87/35/PMC9590112.tar.gz,"[' demonstrates the visualization of benign and malignant lesions using teledermoscopy.', 'Dermoscopic images: (A) Dysplastic Nevus; (B) Melanoma in Situ; (C) Basal Cell Carcinoma (low grade-nodular type) [28].', ' illustrates quality dermoscopic images obtained in the study [28].']",Figure 2 Synchronous teledermoscopy protocol tested at a military training facility. Basic configuration for teledermoscopy. ( ) Dermatoscope; ( ) Cisco Jabber Webcam; ( ) Sanitize dermatoscope and skin lesions with alcohol; ( ) Gentle contact between webcam and dermatoscope [ ].,yes
PMC4065024,Figure_4,oa_package/ba/2e/PMC4065024.tar.gz,"['For six of the proteins identified in the mass spectrometry analysis, including those proteins observed to co-localize with RNA foci in vivo, specificity of interaction with the (GGGGCC)5 RNA was assessed using RNA pull down assays from whole neuronal SH-SY5Y cell extract and western immunoblotting (A).', 'coli (B).', 'Identified RNA-binding candidates interact specifically and directly with GGGGCC5 RNA.', '1093/brain/awu120/-/DC1"">Supplementary ).']","Figure 4 Identified RNA-binding candidates interact specifically and directly with GGGGCC RNA. ( ) Neuronal SH-SY5Y whole cell extract was incubated with either no RNA, AU-rich or GC-rich biotinylated RNA coated onto streptavidin beads before UV-cross linking. Bound proteins were eluted using RNase A and further identified using SDS-PAGE and western immunoblotting with the indicated antibodies. It is noted that the weak signal for SRSF2 is due to difficulty finding an antibody that is efficacious in western immunoblotting. The anti-hnRNP H1/F antibody recognizes both proteins, which are similar ( ). ( ) Hexa-histidine-tagged recombinant SRSF1 11-196, GB1-tagged SRSF2 9-101 and ALYREF full length were expressed in and purified using metal ion affinity chromatography in 1 M NaCl containing buffers to remove potentially bound RNA from ( ). GGGGCC RNA was separately end-labelled with poly nucleotide kinase using [- P]-ATP, before incubation with purified proteins. RNA was covalently bound (+) or not () after UV irradiation. Absence of radioactive signal ( ; PhosphoImage) in absence of UV irradiation demonstrates specificity of direct binding observed after UV treatment. All gels shown in the different panels were exposed simultaneously for the same amount of time (5 h).",yes
PMC4594618,Figure_2,oa_package/ae/0e/PMC4594618.tar.gz,['Leukocyte trafficking in the perimenstrual human endometrium (derived from data published and reviews by Bonatz et al.'],Figure 2 Leukocyte trafficking in the perimenstrual human endometrium (derived from data published and reviews by ; ; ; ). Top panel: Sex steroid profiles in the luteo-follicular transition (perimenstrual window). Bottom panel: Overview of leukocyte traffic in the endometrium with transition from secretory phase through menses/endometrial repair to the proliferative phase of next cycle. Size of cell image reflects abundance.,yes
PMC4648574,Figure_3,oa_package/89/74/PMC4648574.tar.gz,"['Immunohistochemical findingsCK7, Muc5AC (A), p63 (B), and p40 were positive in all 26 PMEC cases (26/26); EGFR was positive in 11 cases (11/26); TTF-1 (C), ALK (D), and HER-2 were negative in all cases (0/26).', 'The Ki-67 (E and 3F) labeling index ranged from 2% to 80% (mean 9.', 'g003Immunostains of PMEC.']",10.1371/journal.pone.0143169.g003,yes
PMC9435650,Figure_2,oa_package/9f/d9/PMC9435650.tar.gz,"['As shown in , treatment of keratinocytes with IL-4 or IL-13 alone and the combination of both IL-4 and IL-13 significantly increased p62 levels in the absence of E P, while there was no difference in p62 levels in the presence of E P.', 'These cytokines markedly reduced the LC3-II amounts in the presence of E P, indicating that IL-4 and IL-13 partially block autophagic flux in keratinocytes (, A C), which is consistent with the in vivo results described in C.', 'Moreover, while the autophagy inducer rapamycin increased the appearance of LC3-positive puncta, an indicator of autophagy occurrence (5), the administration of IL-4 or IL-13 alone as well as their combination significantly diminished the number of LC3-positive puncta in rapamycin-treated keratinocytes (D).', 'Interestingly, treatment of keratinocytes with other Th2 cytokines, such as IL-33 and thymic stromal lymphopoietin (TSLP), affected neither p62 accumulation nor LC3-II levels in the presence of E P (Supplemental , A and B) and did not affect the number of LC3-positive puncta in keratinocytes (Supplemental F), implying that not all Th2 cytokines involved in AD pathogenesis play a role in the autophagy process in keratinocytes.', 'Treatment with the Th1 cytokine interferon- (IFN- ) and the Th17 cytokine IL-17 decreased the accumulation of p62 and increased the LC3-II amounts in keratinocytes, while IL-23 did not show any significant effect (Supplemental , C E).', 'Interestingly, both IFN- and IL-17 significantly increased LC3-II levels in the presence of E P compared with the absence of E P (Supplemental , C and D), suggesting that these cytokines may induce activation rather than inhibition of autophagic flux in keratinocytes.', 'Th2-derived cytokines are involved in the inactivation of autophagy in AD keratinocytes.']","Figure 2 Th2-derived cytokines are involved in the inactivation of autophagy in AD keratinocytes. ( ) Keratinocytes were stimulated for 12 hours with 100 ng/mL IL-4 or IL-13 alone or in combination in the presence (+) or absence () of 10 g/mL E&P; = 3 per group. Representative p62 and LC3 immunoblots (left) and quantification of band intensities (right). GAPDH was used as a loading control. ( ) Keratinocytes were stimulated for 12 hours with or without IL-4 or IL-13 alone or in combination in the presence of 10 M rapamycin (Rap); = 5 per group. Representative immunofluorescence images (left) and quantification of LC3 puncta in keratinocytes (right). Scale bars: 10 m. Mean SD. * < 0.05, ** < 0.01, **** < 0.0001, < 0.05, < 0.001, < 0.0001. Statistical significance was determined by 2-tailed Students test or 1-way ANOVA with Tukeys multiple-comparison test. All of the data are representative of 3 independent experiments.",yes
PMC3470547,Figure_5,oa_package/9d/36/PMC3470547.tar.gz,"['By 24 hours of stimulation with either wild type or the mutant bacteria, similar amounts of TNF were produced (A).', 'In contrast, macrophages exposed to RB50 produced more IL-6 and IL-1 than those exposed to RB50 clpV (B C), suggesting that the T6SS may stimulate IL-6 and IL-1 production independently of TNF induction.', 'Not surprisingly, IL-17, known to be induced by IL-1 , was also more up-regulated in RB50 stimulated macrophages compared to macrophages stimulated by the clpV mutant (D).', 'We observed that wild type bacteria induced more IL-10 production than the clpV mutant and very low levels of IFN- , suggesting that a mutant lacking clpV may affect cell recruitment by altering IL-10 production (E F).', 'g005ClpV contributes to cytokine production in vitro.']",10.1371/journal.pone.0045892.g005,yes
PMC6747109,Figure_3,oa_package/30/40/PMC6747109.tar.gz,"['In the neonatal administration study, serum levels of mono-sulfated KS in treated mice moderately decreased, compared to those in untreated MPS IVA mice at 8 weeks of age and significantly decreased at 12 weeks of age (A).', 'Serum diHS-0S levels were similar between untreated and treated mice at 8 and 12 weeks of age (B).', 'The histopathologic scores in the treated group were lower than in the untreated group at 12 weeks of age (C).', 'Serum glycosaminoglycan levels and bone pathology of MPS IVA mice after repeated administration of thermostable keratanase.']","Figure 3 Serum glycosaminoglycan levels and bone pathology of MPS IVA mice after repeated administration of thermostable keratanase. MPS IVA mice were treated with 2 U/kg of thermostable keratanase intravenously 3 times at 0, 4, and 8 weeks of age, and PBS was administered into MPS IVA mice (untreated) and heterozygous mice (control) in the same manner. Serum samples were collected from the superficial temporal vein at the time points of 8 and 12 weeks of age. Tissues were collected at 12 weeks of age. ( , ) The level of serum mono-sulfated KS and diHS-0S was measured by LC-MS/MS. = 79. ( ) Bone pathology in growth plate regions of femur and tibia was evaluated by toluidine blue staining. Statistics were analyzed by one-way ANOVA with the Bonferronis post-hoc test. Data are presented as mean SD. * < 0.05 vs. untreated MPS IVA. HS: heparan sulfate, KS: keratan sulfate, TSK: thermostable keratanase.",yes
PMC10248353,Figure_1,oa_package/73/c3/PMC10248353.tar.gz,"['Internal or visceral abdominal injuries are found in up to one-third of patients with a positive seatbelt sign, thus requiring close inspection when they are identified () [12].', 'J Vasc Interv Radiol2021325865923355130526\nSlaterSJ\nLukiesM\nKavnoudiasH\nZiaA\nLeeR\nBoscoJJ\n\nImmune function and the role of vaccination after splenic artery embolization for blunt splenic injuryInjury20225311211534565618Right lateral impact trauma.']",Fig. 1 Right lateral impact trauma. Contrast-enhanced axial scan (soft tissue and lung windows). Comminuted right iliac wing fracture (arrow) with an associated pelvic/gluteal hematoma (asterisks). Tear of the right iliacus muscle with herniating retroperitoneal fat (arrow). Lung windows demonstrating a displaced rib fracture (arrow) with a right pneumothorax (asterisk).,yes
PMC2566572,Figure_2,oa_package/81/d3/PMC2566572.tar.gz,['Low grade papillary urothelial carcinoma infiltrating pelvic bone.'],Figure 2 At low magnification (10 10) the low grade urothelial carcinoma forms nests and infiltrates cortical bone.,yes
PMC10807035,Figure_3,oa_package/41/df/PMC10807035.tar.gz,"['The application technique, according to the manufacturer s instructions, is illustrated step-by-step for treatment of an early carious lesion on a first permanent molar during the eruption period () [23,32,49].', '(a e) Clinical application of P11-4: (a) initial active carious lesion in a permanent molar; (b) cleaning of organic debris with 3% sodium hypochlorite; (c) etching with 35% phosphoric acid; (d) application of P11-4 and waiting for 3 5 min for diffusion into the lesion; (e) fluoride varnish application.']",Figure 3 ( ) Clinical application of P -4: ( ) initial active carious lesion in a permanent molar; ( ) cleaning of organic debris with 3% sodium hypochlorite; ( ) etching with 35% phosphoric acid; ( ) application of P -4 and waiting for 35 min for diffusion into the lesion; ( ) fluoride varnish application.,yes
PMC3343395,Figure_4,oa_package/99/47/PMC3343395.tar.gz,[],Figure 4 Coronal CT showing extent of lesion,yes
PMC10763722,Figure_2,oa_package/dc/49/PMC10763722.tar.gz,"['Next, we analyzed the titin expression profiles of the myocardial samples with high-resolution gel electrophoresis ().', 'The N2BA/N2B titin isoform ratio was elevated in both the DCMTTNtv and DCMTTNtv+ groups compared with the physiological ratios obtained from data in the literature (27) (B).', 'The full-length titin to myosin heavy-chain ratio (T1/MyHC) was significantly decreased in the DCMTTNtv+ group (C).', 'The ratio of the T2 fragment (calpain-dependent proteolytic fragment that encompasses titin s A-band section and a 100 200 kDa portion of its distal I-band section (28)) to full-length titin (T2/T1) was significantly increased in the DCMTTNtv+ samples (D), suggesting that proteolytic activity may be increased in the DCMTTNtv+ myocardial tissue.', 'Nevertheless, the (T1 + T2)/MyHC ratio was also significantly reduced in the DCMTTNtv+ samples (F).', 'We detected additional protein bands in the DCMTTNtv+ group (A and Supplemental ), albeit not in all TTNtv+ samples.', 'Notably, upon adding the truncated protein quantity to the respective T1 (E), we observed no significant difference between the DCMTTNtv+ and DCMTTNtv samples.', 'Furthermore, the integrated titin quantities (T1 + T2 + truncated titin) normalized to MyHC were essentially identical in DCMTTNtv+ and DCMTTNtv (G and Supplemental Table 6).', 'Furthermore, the supernatants of the washed myofibril samples were devoid of truncated titin (Supplemental B).', 'Importantly, however, we found that the integral titin amount, which included the full-length, truncated, and proteolysed proteins, was comparable in the DCMTTNtv and DCMTTNtv+ samples ().', 'The truncated proteins were revealed on the gels at the molecular weight levels expected, based on the NGS data (A and Supplemental , and Supplemental Tables 5 and 6).', 'The difference in titin expression in DCMTTNtv and DCMTTNtv+ samples, calculated as the T1/MyHC ratio (C), was alleviated if the truncated proteins were taken into account in calculating total titin in the DCMTTNtv+ samples (, E and G).', 'Titin isoform proteomics analysis.', 'Grayscale saturation was avoided by measuring OD, and technical variability was negligible (see Supplemental , C F).', 'In contrast to the TTNtv sample (patient 7), the TTNtv+ myofibril sample (patient 75) contained truncated titin, suggesting that the truncated protein was part of the sarcomere (see also Supplemental A).', 'In further support of this observation, the concentrated supernatants of the washed and centrifuged myofibril samples were devoid of detectable amounts of truncated titin (Supplemental B).', 'The gel image shown here is a spliced duplicate of the full gel shown in Supplemental A.']","Figure 2 Titin isoform proteomics analysis. ( ) Representative image of gel electrophoresis demonstrating titin N2BA and N2B isoforms, titins proteolytic degradation product (T2), and MyHC in TTNtv and TTNtv human myocardium and rat myocardium. The red arrow indicates the truncated protein. Linear contrast adjustment was applied to the original image for better visualization. The dotted line indicates where the different lanes of the same gel were digitally spliced together. ( ) The N2BA/N2B ratio did not differ between TTNtv ( = 19) and TTNtv ( = 98) DCM groups. ( ) Total titin (T1 = N2BA + N2B) normalized to MyHC was significantly reduced in the DCM samples. ( ) T2/T1 increased significantly in the TTNtv DCM samples. ( ) Although T1/MyHC was reduced in the TTNtv DCM group, no differences were seen in the samples if the truncated (trunc) protein and T2 were added to T1. Values indicate the mean SEM. Samples were statistically compared by unpaired, 2-tailed Students test; * < 0.05. Grayscale saturation was avoided by measuring OD, and technical variability was negligible (see , CF). For proteomics data statistics, see .",yes
PMC10509807,Figure_5,oa_package/3d/ae/PMC10509807.tar.gz,"['The procedure was successful with satisfactory deployment of the construct, clear flow diversion away from the aneurysm, and desirable stagnation within the aneurysm ().', 'Posterior left internal carotid artery aneurysm stenting (white arrow showing the stent).', '3DiscussionPetrous ICA aneurysms have various etiologies, which include congenital factors related to developmental defects in the middle layer of the artery, blunt or penetrating trauma, iatrogenic causes, chronic infection, inflammation, and radiation exposure.']",Fig. 5 Posterior left internal carotid artery aneurysm stenting (white arrow showing the stent). There is a clear flow diversion away from the aneurysm and accompanying stagnation within it (white asterisk representing the aneurysm).,yes
PMC6682487,Figure_2,oa_package/db/3c/PMC6682487.tar.gz,"['org/1999/xlink"" xlink:href=""10-1055-s-0039-1694735-i1800071oa-1""/>\nThe picture shows a leukoplakia vaporization on soft palate by carbon dioxide (CO\n2\n) laser.']",Fig. 2 The picture shows a leukoplakia vaporization on soft palate by carbon dioxide (CO ) laser. ( ) Lesion before vaporization. ( ) Lesion just after vaporization. ( ) Lesion 3 weeks after vaporization.,yes
PMC7886835,Figure_5,oa_package/e1/f1/PMC7886835.tar.gz,"[' 5 and Video 1.', 'Mixed reality holograms of MD-DVS.', 'The high-dose area on the back in 3D did not obscure the background presents results of the subjective workload scores from NASA-TLX.']","Fig. 5 Mixed reality holograms of MD-DVS. ( ) The left image shows the HoloLens user view, with appearance sharing and similar looking skin dose holograms with another HoloLens user. The value shown on the center represents the users own eye lens exposure dose at the current position. The color bar shows the patient skin dose levels. ( ) The right image shows projected skin dose distribution. The high-dose area on the back in 3D did not obscure the background",yes
PMC8611515,Figure_1,oa_package/7f/fd/PMC8611515.tar.gz,"['On physical examination, he had mild, tender left anterior cervical lymphadenopathy with two hard nodular erythematous formations associated with a violaceous plaque with satellite papules and ulceration in the area of the manubrium of sternum ().', '.']",Figure 1. Patient at presentation. Left cervical lymphadenopathy associated with a violaceous plaque with satellite papules and ulceration in the area of the manubrium sternum.,yes
PMC5612025,Figure_6,oa_package/77/8b/PMC5612025.tar.gz,[],Fig. (6) Bankart repair plus posteroinferior capsule plication in a right shoulder.,yes
PMC3741920,Figure_8,oa_package/71/5d/PMC3741920.tar.gz,"[' shows the identified atypia regions in Grade 2 tissue image.', '007""/>(a) Grade 2 tissue image, (b) atypia regions that are generated by region R\nO and region R\nI of (a).']","Figure 8 (a) Grade 2 tissue image, (b) atypia regions that are generated by region and region of (a).",yes
PMC11518684,Figure_4,oa_package/e6/5e/PMC11518684.tar.gz,[],Figure4 Tumor at the top of the renal pelvis.,yes
PMC2566572,Figure_1,oa_package/81/d3/PMC2566572.tar.gz,['CT scan of pelvis showing a large locally destructive mass lesion.'],Figure 1 . Showing a right sided large heterogeneous pelvic mass with an area of central necrosis with evidence of bone destruction (right acetabular invasion) and distal rectal involvement.,yes
PMC9319040,Figure_7,oa_package/fd/b9/PMC9319040.tar.gz,"['The results in show no significant differences between the groups expression levels of the studied molecules.', 'Association of the gene expression of BAFF, APRIL, and their receptors (R-BAFF, TACI, and BCMA) with anti-HLA antibodies status.']","Figure 7 Association of the gene expression of BAFF, APRIL, and their receptors (R-BAFF, TACI, and BCMA) with anti-HLA antibodies status. Comparison of BAFF ( ), APRIL ( ), R-BAFF ( ), TACI ( ), and BCMA ( ) gene expression levels in peripheral blood samples among KTRs without anti-HLA antibodies (black, = 30), preformed anti-HLA antibodies (blue, = 6) at pre-transplantation, and with de novo DSA antibodies (gray, = 4). The HPRT gene was used as an endogenous control to normalize BAFF and APRIL gene expression, and the CD19 to normalize R-BAFF, TACI, and BCMA gene expression. Expression data are represented as the mean SEM. Statistical analyses were performed using the KruskalWallis test and Dunns test with Bonferroni correction for multiple comparisons. Values < 0.05 were considered significant.",yes
PMC9157357,Figure_1,oa_package/0c/9e/PMC9157357.tar.gz,"['On examination, the swelling over the malar prominence was mildly indurated and green-coloured purulent pus could be expressed from right side (figure 1).', 'After tarsorrhaphy, a bilateral lateral rhinotomy with Dieffenbach s lateral extension to lower eyelid incision (batwing incision) made with inclusion of diseased skin (figure 1).', 'Markings of incision, a bilateral-limited lateral rhinotomy with extended Dieffenbach s approach (batwing incision).']","Figure 1 Markings of incision, a bilateral-limited lateral rhinotomy with extended Dieffenbachs approach (batwing incision). Arrow showing inclusion of fistulous site in incision.",yes
PMC1712336,Figure_2,oa_package/5f/56/PMC1712336.tar.gz,"['Early stage of progressive vertebral fusion in which C4-C6, showed progressive anterior disc narrowing and end plate irregularities (arrows; a-b), whereas (arrow c) showed the development of a thick anterior and posterior bony ridge.']","Figure 2 Early stage of progressive vertebral fusion in which C4-C6, showed progressive anterior disc narrowing and end plate irregularities (arrows; a-b), whereas (arrow c) showed the development of a thick anterior and posterior bony ridge.",yes
PMC2988902,Figure_1,oa_package/61/79/PMC2988902.tar.gz,"['A STAT portable chest and abdominal radiograph demonstrated a massively dilated stomach with decompressed loops of small bowel (fig. 1).', 'A case reportAngiology2007581021051735116510MunarrizRHwangJGoldsteinITraishAMKimNNCocaine and ephedrine-induced priapism: case reports and investigation of potential adrenergic mechanismsUrology2003621871921283746411RezkallaSHMesaJSharmaPKlonerRAMyocardial infarction temporally related to ephedra - a possible role for the coronary microcirculationWMJ200210164661242692412SamenukDLinkMSHomoudMKContrerasRTheoharidesTCWangPJEstesNAAdverse cardiovascular events temporally associated with ma huang, an herbal source of ephedrineMayo Clin Proc20027712161179524913NyskaAMurphyEFoleyJFCollinsBJPetrankaJHowdenRHanlonPDunnickJKAcute hemorrhagic myocardial necrosis and sudden death of rats exposed to a combination of ephedrine and caffeineToxicol Sci2005833883961553774414AshleySWSonnenscheinLACheungLYFocal gastric mucosal blood flow at the site of aspirin-induced ulcerationAm J Surg19851495359396664215BinmoellerKFBennerKGEmphysematous gastritis secondary to gastric infarctionAm J Gastroenterol199287526529155394316KawanoSMasudaETsujiSNaganoKFusamotoHKamadaTEthanol causes vasoconstriction due to endothelin-1 release in rabbit gastric vesselsMicrovasc Res199141408410207287217MasudaEKawanoSNaganoKEffect of ethanol on endothelin-1 release from gastric vasculatureGastroenterol Jpn199126suppl 38182188496718MasudaEKawanoSNaganoKTsujiSIshigamiYTsujiiMHayashiNFusamotoHKamadaTEffect of intravascular ethanol on modulation of gastric mucosal integrity: possible role of endothelin-1Am J Physiol1992262G785G790159038819PetteiMJLevyJAbramsonSNonocclusive mesenteric ischemia associated with propranolol overdose: implications regarding splanchnic circulationJ Pediatr Gastroenterol Nutr199010544547197274320PfeifferCJKeithJCChoCHDeRolfSPfeifferDCMisraHPGastric and cardiac organoprotection by lidocaineActa Physiol Hung1989731291362596305Preoperative abdominal radiograph demonstrated a massively dilated stomach with otherwise normal bowel gas pattern.']",Fig. 1 Preoperative abdominal radiograph demonstrated a massively dilated stomach with otherwise normal bowel gas pattern.,yes
PMC4840569,Figure_1,oa_package/da/e8/PMC4840569.tar.gz,[':Ultrasonographic appearance of the right iliac fossa.'],Figure 1: Ultrasonographic appearance of the right iliac fossa. An ill-defined hypoechoic region surrounding a tubular non-compressible structure (maximum calibre 7.15mm) was identified. This appearance was thought most likely to represent perforated appendicitis with no definable walled-off collection.,yes
PMC8848767,Figure_4,oa_package/5d/ae/PMC8848767.tar.gz,"['On gross examination, the excision biopsy was round, yellowish-white tumour covered by a thin fibrous capsule ().', 'Complete excision biopsy of the tumour.']",Figure 4 Complete excision biopsy of the tumour. The resected material was a round yellowish-white mass measuring ~7x11x6 mm in size and was covered by a thin capsula fibrosa.,yes
PMC8292955,Figure_3,oa_package/ea/af/PMC8292955.tar.gz,[],Figure3 Pleomorphic adenoma (PA) in contrast-enhanced ultrasound (CEUS). The qualitative CEUS showing the mass with low intensity and heterogeneous enhancement and echo-free areas. The perfusion pattern of contrast agent was slow in and fast out ( -early phase; -middle phase; -late phase). H&E stain (original magnification 100).,yes
PMC11416467,Figure_2,oa_package/29/8e/PMC11416467.tar.gz,"['Left hip CT done the next day () revealed a small linear intra-articular osseous fragment in the hip joint space medially with resultant joint space widening.', ':Axial proton-density fat saturated MRI of the left hip demonstrates low-signal intensity tissue consistent with the avulsed posterior labrum and contiguous acetabular epiphyseal fragment entrapped between the femoral head and acetabulum (white arrow).']","Fig. 2 Axial CT scan of the left hip demonstrates a small intraarticular osseous fragment interposed between the femoral head and the acetabulum (white arrow). Also noted is abnormal joint space widening, as well as absence of the posterior rim of the left acetabulum, which represents the donor site of the interposed fragment.",yes
PMC4557097,Figure_6,oa_package/1a/02/PMC4557097.tar.gz,['Cathepsin B expression occurs in selected regions of the brain.'],"Figure 6 . are micrographs of the same coronal mouse brain section and show tissue structure and cathepsin B mRNA expression, respectively. In , the section is nissl stained, which highlights neurons as dark blue. In , hybridization of sections with antisense mRNA to cathepsin B illustrates the brain regions of cathepsin B mRNA expression. Hotter colors, such as yellow and red, signify high expression, cooler colors, such as green and blue, indicate low expression, and black indicates undetectable expression. A comparison of the two micrographs shows that cathepsin B is intensely expressed in the hippocampal neuronal cell layer (arrows) and in the cortex (box). Figures taken from the Allen Brain Institute web site .",yes
PMC4006327,Figure_3,oa_package/e4/b7/PMC4006327.tar.gz,[],Fig.3 Erythematous plaques covered with tense blisters with clear fluid-clinical aspect of bullous pemphigoid,yes
PMC9715374,Figure_7,oa_package/43/8d/PMC9715374.tar.gz,['No.'],"Figure 7 No. 1 of the blood clot group. (A) Decrease in the apical foramen diameter, (B) increase in root wall thickness, and (C) increase in root length.",yes
PMC9390875,Figure_4,oa_package/67/10/PMC9390875.tar.gz,[],"FIGURE 4 Therapeutic strategies targeting neutrophil extracellular trap (NET) formation. Regulating uncontrolled process of NET formation (NETosis) could be a promising strategy for the treatment of NETrelated diseases. DNase and multiple inhibitors can be utilized for targeting the critical steps and products of NETs. Abbreviations: CitH3, citrullination of histone H3; MPO, myeloperoxidase; NE, neutrophil elastase; NOX, NADPH oxidase complex; PAD4, peptidyl arginine deiminase 4; ROS, reactive oxygen species; TLR, tolllike receptor",yes
PMC6238727,Figure_2,oa_package/1d/06/PMC6238727.tar.gz,"['Dual-probe 3 was cleaved by target (i) thrombin and (ii) MMPs, specificity was confirmed by MALDI TOF MS.', 'The enzymatic cleavage of the dual-probe was specific; with MALDI-TOF MS confirming cleavage between Gly Nle by MMPs and between Arg Gly by thrombin (see ).', 'Activation of dual-probe 3 was prevented by marimastat (a pan-MMP inhibitor) and anti-thrombin III (AT3) ().', 'S3a ) and completely plasmin and Factor Xa resistant, whilst retaining its ability to be activated by target enzymes showing high signal to noise (a).', '2-fold following the addition of thrombin with the fold-changes in fluorescence superior to those of individual thrombin and MMP compounds b and f (b).', 'Vitally, no signal increase was observed as a consequence of cross-enzyme reactivity, and no steric hindrance was seen when cleaving the dual-probe with MMP and thrombin either simultaneously (a), or sequentially (', '9 Da indicates combined thrombin and MMP cleavage ( and ']","Fig. 2 Dual-probe was cleaved by target (i) thrombin and (ii) MMPs, specificity was confirmed by MALDI TOF MS. (a) Dual-probe was incubated with target and off-target enzymes, with or without inhibitor. Activation was determined by measuring the fluorescence increase (compared to enzyme-free control) at 10 min at two different wavelengths (ex/em 485/528 nm (FAM) and 640/670 nm (Cy5)). (b) The fold-change in fluorescence intensity of fragments , and dual-probe were compared following activation with MMP-9 or thrombin, with probe showing specific FAM activation with thrombin and Cy5 increasing with MMP-9 (c) structure of dual-probe showing the theoretical molecular weights of the fragments obtained following cleavage; (d) MALDI-TOF MS spectra of before and after treatment with thrombin or MMP-9. The initial peak for at / 6182.001 Da (calc. for C H N O S [M ]: 6182.353 Da) disappears following treatment showing two new peaks with the expected / . (e) MALDI-TOF MS ( / 15003000 Da) correlates well with the expected fragments even when the two enzymes act together. Data is the mean of three independent replicates performed in duplicate. Error bars represent s.e.m. Statistical analysis was performed with one-way ANOVA test. (a) * = 0.0225; ** = 0.0026; **** < 0.0001. (b) ** = 0.0018; *** = 0.0004; **** < 0.0001.",yes
PMC4034155,Figure_4,oa_package/3d/8f/PMC4034155.tar.gz,[],"FIGURE 4 . Alv, alveus; DG, dentate gyrus; Hf, hippocampal fissure; IML, inner molecular layer; Lac-mol, stratum lacunosum-moleculare; Mol, molecular layer; OML, outer molecular layer; Or, stratum oriens; Pyr, stratum pyramidale; Rad, stratum radiatum; WM, white matter; Scale bar : 250 m in . Taken from .",yes
PMC10084420,Figure_3,oa_package/f1/54/PMC10084420.tar.gz,[],"FIGURE 3 Effect of green light pretreatment on UVBtriggered dermal matrix degradation in MDFs. Immunoblot analysis of MMP1, collagen I and collagen III at 72h following the final UVB exposure. actin expression was utilized for normalization. Data are meanSD from triplicate assays (* <0.05)",yes
PMC8994011,Figure_5,oa_package/1d/05/PMC8994011.tar.gz,"[' 5).', 'Overall correlations of right liver alveolar echinococcosis in a 54-year-old women.', 'D Correlation with diffusion-weighted imaging showing peripheral hypersignal of AE lesion with low ADC valuesTable 2Differential diagnosis for liver calcificationsDifferential diagnosis for liver calcificationsPrevalencePattern of calcificationsNotesParasitic infectionsAlveolar echinococcosisVery common at all stagesDiffuse Scattered FocalPatient from endemic areasHypoattenuating infiltrative mass, absence of contrast enhancementCystic echinococcosisMay be observed at all stagesConcerns the cyst wallPatient from endemic areasCircumscribed liver cyst with heterogeneous content (detached membrane or internal cysts)CE1: rareSprinkledCE2: commonSprinkled eggshell-like = circular or contentCE3: commonEggshell-like sprinkled = circular or contentCE4: commonEggshell-like sprinkledCE5: very commonCircular or content = eggshell-like sprinkledPartially or entirely calcified cystCystic lesionsCystadenoma/cystadenocarcinoma8 to 25%Coarse mural and septalCystic lesion with enhanced septaWall nodule in cystadenocarcinomaLiver cystsRareNon-circumferentialNon-enhancing simple cystic lesionSolid tumorsCholangiocarcinomaRareSolitary or multipleIll-definedInfiltrative mass with delayed enhancementHepatocellular carcinoma (HCC)Rare (more common after therapy)VariableEarly enhancement and wash out of solid component in cirrhotic liverFibrolamellar HCCVery common (40 to 70%)Calcified central scareYoung adult, large lobulated lesion with central scar in normal liverMetastasisCommon in Mucinous carcinoma/osteosarcoma Treated metastasisVariableMultiple lesionsHemangiomaRareMore frequent if big sizeCentral and coarsePeripheral discontinuous enhancement, then centripetalMagnetic resonance imaging (MRI)MRI provides more precise information concerning the characterization of the lesion, with a heterogeneous infiltrative hypovascular mass that is a mix of solid and cystic tissue.', ' 5).', ' 5, 9).']",Fig. 5 Overall correlations of right liver alveolar echinococcosis in a 54-year-old women. Photograph of gross specimen after right hepatectomy showing multiples microcysts with honeycomb aspect. T2-weighted MR image in the axial plane showing typical microcysts in correlation with gross specimen (arrows; Kodama 2). FDG-PET/CT scan showing active parasitic lesion with increased FDG-uptake at the periphery of the lesion. Correlation with diffusion-weighted imaging showing peripheral hypersignal of AE lesion with low ADC values,yes
PMC5972287,Figure_7,oa_package/d3/01/PMC5972287.tar.gz,['Reduced splenic expression of genes required for macrophage response to pathogens.'],"Figure 7 Reduced splenic expression of genes required for macrophage response to pathogens. Analysis of toll-like receptor (TLR) transcripts show downregulated expression of the majority of genes 15days after middle cerebral artery occlusion (MCAO) in comparison to sham-operated animals = = = . RT-qPCR for confirmed significant downregulation at 25days after MCAO in comparison to sham-operated animals ( = = = = = = ). Expression of transcripts which encode macrophage-associated co-stimulatory proteins important for activating the adaptive immune response show downregulated expression 15days after MCAO in comparison to sham-operated animals = = = . RT-qPCR for confirmed significant downregulation at 25days after MCAO in comparison to sham-operated animals ( = = = = = = ). Expression of which encodes the cytokine TNF was downregulated 15days after experimental stroke whereas expression of which encodes the cytokine IL-10 remained relatively unchanged = = = . RT-qPCR for confirmed downregulation of expression 17days after MCAO in comparison to sham-operated animals ( = = = = = = ). CBA assay of homogenized spleen detected TNF in the spleens of sham-operated animals and animals recovered 7days after MCAO; however, this was undetectable in spleens from mice recovered 15days after MCAO. Data show meanSD , * <0.05; ** <0.01; one-way analysis of variance with Bonferonni correction.",yes
PMC3898319,Figure_8,oa_package/ea/d7/PMC3898319.tar.gz,"['felis, the most analogous early mucosal defect is cystic gland dilatation (a), which is considered to be part of the inflammation-metaplasia-malignancy continuum in this species.', 'Glands exhibiting mucous cell metaplasia, characterized by the replacement of parietal cells with large foamy mucus secreting cells (b, arrowed) staining positively with Alcian blue were quantified.', '05, c).', 'org/1999/xlink"" xlink:href=""onc2013334f7""/>Photomicrographs and quantitative scoring of epithelial pathology in mice untreated or infected with H.']","Figure 8 Photomicrographs and quantitative scoring of epithelial pathology in mice untreated or infected with for 1 year. ( ) Cystic gland dilatation. ( ) Mucous cell metaplasia, arrow marks highlight mucous cells with foamy cytoplasm. ( ) Gastric epithelial dysplasia. Individual animal scores and median plotted. signifies significant difference from untreated mice of same genotype. signifies significant difference from WT mice subjected to the same treatment, <0.05. * indicates significant difference to WT animals, <0.05. Scale bar 50m.",yes
PMC5759315,Figure_3,oa_package/42/23/PMC5759315.tar.gz,"['MLPS typically develops in the proximal extremities, with two thirds of cases originating in the thigh ().', '5.']",Fig 3. Radiologic appearance of myxoid liposarcoma of the proximal thigh. (A) Coronal T2-weighted magnetic resonance image of 80-mm tumor (arrow) with characteristic high signal intensity. T1-weighted fat-suppressed axial magnetic resonance images of (B) pregadolinium and (C) postgadolinium contrast show avid and heterogeneous enhancement of tumor (arrow).,yes
PMC10782191,Figure_2,oa_package/f3/b2/PMC10782191.tar.gz,[],Figure 2 Angiography showing occluded left vertebral artery as well as the extensive collateralization,yes
PMC6761632,Figure_2,oa_package/b3/93/PMC6761632.tar.gz,['CardioGRKO male and female mice develop spontaneous left ventricular remodeling.'],"Figure 2 Cardio male and female mice develop spontaneous left ventricular remodeling. , Representative images of intact hearts from 6monthold male and female control and cardio mice. , Longitudinal hematoxylin & eosinstained heart sections from 6monthold male and female control and cardio mice. Arrows () point out to increased left ventricle size and the presence of a thrombus in the left atria of the female cardio heart. The images are representative of 5 mice per group. CardioGRKO indicates cardiomyocytespecific glucocorticoid receptor knockout.",yes
PMC6718379,Figure_3,oa_package/68/d9/PMC6718379.tar.gz,"['3a), while no differences were observed between 2-month-old rats across genotypes (Supplementary ', '3b).', '3c), confirming that GIRK/Kir3 channel function in DA neurons is impaired.', 'DA sensitivity and intracellular [Ca2+] are altered in 4-month-old Syn DA neurons.', 'Source data are provided as a Source Data fileOverall, the alterations in K+ conductances, apamin-sensitive AHC and cell firing in Syn DA neurons, at an age preceding cell degeneration, are all indications of cellular dysfunction.', '3d), whereas no changes were detected in 2-month-old animals (Supplementary ', '3e).', '3f), suggesting that at 60 mV the contribution of VGCCs to the higher [Ca2+] in Syn DA neurons is minimal.', '3g), providing an estimate of the Ca2+ accumulated in the stores.', '3g), suggesting lower Ca2+ content in CPA-sensitive stores and impaired storing capacity that could explain the cytosolic Ca2+ accumulation in Syn DA neurons.']","Fig. 3 DA sensitivity and intracellular [Ca ] are altered in 4-month-old Syn DA neurons. Extracellular recordings in SNpc DA neurons from 4-month-old rats and response to 2min application of 30M DA (scale: 1min, 0.2mV). Expanded traces (scale: 1s) show firing in control condition and during DA. The plot shows the firing reduction (% of control) after DA (23 cells from 8 WT, 34 cells from 10 Syn; MannWhitney test *** <1.0010 ). DA currents (30M, 2min) during voltage clamp (60 mV; scale: 2min, 20 pA) and mean (s.e.m.) doseresponse curves (18 cells from 8 WT, 18 cells from 7 Syn; two-way ANOVA: genotypeconcentration, F =0.66, =0.586; concentration, F =6.68, =0.001; genotype, F =8.63, P=0.006; WT vs Syn: 1M, >0.999, 3M =0.175, 10M, ** =0.007, 30M * =0.012 with Bonferronis). Baclofen-mediated currents (10M, 3min; 60 mV; scale: 2min, 50 pA), and mean (s.e.m.) current amplitude (11 WT cells, 13 Syn cells, 3 rats each; two-way ANOVA: genotypeconcentration, F =4.78, =0.041; concentration, F =23.96, <1.0010 ; genotype, F =16.04, =7.0010 . WT vs Syn: 1M, =0.337, 10M, ** =0.001 with Bonferronis). Infrared videomicroscopy of a patched DA neuron and a Fura-2 ratiometric image (scale: 10 m) showing variations in cytosolic [Ca ]. The plot shows cytoplasmic [Ca ] in DA neurons at 60 mV (23 WT, 26 Syn cells, 7 rats each; *** <1.0010 , Welchs -test). Somatic Ca at 60 mV in control conditions and in the presence of Ca -free bath solution (scale: 2min). The plot shows the level of [Ca ] reduction (11 WT neurons from 4 rats, 11 Syn neurons from 5 rats; =0.338 with Welchs -test). [Ca ] levels in DA neurons from slices incubated with isradipine (200nM, 1h; 10 WT, 6 Syn neurons, 3 rats each; *** =1.0010 with Welchs -test. Transient rise in fluorescence ratio following CPA application (10M; scale: 2min)and plot showing smaller CPA-induced Ca increase in Syn DA neurons (5 WT, 9 Syn neurons, 3 rats each; *** =0.001 with MannWhitney test). Source data are provided as a Source Data file",yes
PMC8133511,Figure_1,oa_package/30/a1/PMC8133511.tar.gz,"['Rhabdomyoma arising from lateral wall of left ventricle and protruding into the left ventricular cavityParasternal long axis view showing rhabdomyoma arising from lateral wall of left ventricle and protruding into the left ventricular cavityDiscussionCardiac rhabdomyoma is usually diagnosed in infancy, with 70% to 90% of these children having TSC [3-5].']",Figure 1 Rhabdomyoma arising from lateral wall of left ventricle and protruding into the left ventricular cavity,yes
PMC11311325,Figure_3,oa_package/22/21/PMC11311325.tar.gz,"[' illustrates the semiquantitative and quantitative pathological evaluation results (all analysis results are provided in Supplementary Tables S1 and S2, with a summary in Supplementary Table S3), and shows representative pathological microphotographs of ATTR deposition in both sides of the atrial regions.', 'In the VS, ATTR deposition in the myocardial interstitium was severe, whereas that in the endocardial interstitium was mild (a).', 'Similar to our previous study [12], the latter was significantly more severe on the right side (b).', 'ATTR deposition severity in the superior vena cava (SVC) was similar to that in the AS (c).', 'In the semiquantitative analysis, 14 out of 20 cases showed significantly more severe ATTR deposition in the LA regions than in the RA regions (d,e).', 'Our quantitative analysis further supported left-side predominant ATTR deposition in the myocardium (g).', 'Meanwhile, although AANF deposition was generally more severe in the LA regions than in the RA regions, the difference was not statistically significant (f).', 'Severity of cardiac involvement in each region of the heart.']","Figure 3 Severity of cardiac involvement in each region of the heart. ( ) Semiquantitative grading of ATTR deposition; ( ) semiquantitative grading of AANF deposition; ( ) quantitative grading of ATTR deposition in the myocardium. ( ) Ventricular septum (VS); ( ) atrial septum (AS); ( ) superior vena cava (SVC); ( ) right atrium (RA) and left atrium (LA); ( ) right atrial appendage (RAA) and left atrial appendage (LAA); ( ) AS, both atrial regions and SVC; ( ) VS, AS, both atrial regions and SVC. Abbreviations: E, endocardium; EL, endocardium of the left side; ER, endocardium of the right side; I, interstitium; IL, interstitium of the left side; IR, interstitium of the right side; V, vessel; VL, vessel of the left side; VR, vessel of the right side. ** < 0.01, compared using the MannWhitney U test.",yes
PMC3893586,Figure_4,oa_package/04/d2/PMC3893586.tar.gz,['Immunostaining of Pukinje cells of the cerebellum (a) and pyramidal cells of CA4 region of hippocampus (b) for poly-PR protein shows strong immunoreactivity of chromatin.'],Figure 4 Note lack of staining of NCI in either cell type. Immunoperoxidasehaematoxylin. All40 microscope objective magnification.,yes
PMC10861404,Figure_10,oa_package/82/45/PMC10861404.tar.gz,[],,yes
PMC10706067,Figure_3,oa_package/6b/29/PMC10706067.tar.gz,"['Female patient, 73 years old, who has been diagnosed with mandibular osteochemonecroses.']","Figure 3 Female patient, 73 years old, who has been diagnosed with mandibular osteochemonecroses. She has been under bisphosphonate treatment for over 3 years for metastatic breast cancer and presents with mandibular pain, swelling and intraoral bone exposure. The profile image acquired 4 h after the intravenous injection of anti-granulocyte antibodies shows marked radiotracer uptake in the left mandibula (( ), red arrow). The blood pool phase (anterior view) of the bone scan shows left mandibular hyperemia (( ), red arrow). Likewise, the delayed image of the bone scan again shows marked radiotracer uptake (( ), red arrow). For this patient, the treatment plan involves antibiotic treatment, sequestrectomy, or possible mandibular resection and reconstruction with a fibula free flap depending on the residual bone quality.",yes
PMC7451173,Figure_3,oa_package/81/fe/PMC7451173.tar.gz,"['At the time of referral, there was radiographic evidence of lesion regrowth at the site of hippocampal disease, concerning for tumor recurrence versus pseudo-progression [a and b].', 'Postoperative MRI obtained at 2 weeks after LITT showed mildly increased size of the enhancing lesion but similarly decreased size of associated edema [c and d], and the patient was otherwise at baseline speech difficulty off steroids.', 'At 2-month follow-up, changes were more evident with stable to mildly decreased size of the enhancing lesion but more pronounced reduction of perilesional edema [e and f].', ':Clinical imaging for case illustration of recurrent glioblastoma.']",Figure 3: Clinical imaging for case illustration of recurrent glioblastoma. Preoperative (a) T1-weighted postcontrast and (b) T2- weighted FLAIR magnetic resonance imaging (MRI) demonstrating an enhancing lesion in the left medial temporal region with surrounding edema. Imaging obtained 2 weeks after laser interstitial thermal therapy (LITT) showed a (c) mildly increased size of the enhancing lesion and (d) mild reduction of perilesional edema. An MRI obtained 2 months after LITT demonstrated more definitive (e) reduction in size of the enhancing lesion and (f) further diminishment of edema.,yes
PMC10635349,Figure_1,oa_package/71/38/PMC10635349.tar.gz,"['As was true for endogenous murine A [9], insoluble (guanidinium-extracted) forms of human A 40 and A 42 were significantly increased in ~ 3-week-old CatD-KO, hAPP-positive (KO+) mice relative to hAPP-positive mice with two (WT+) or one (HET+) functional copies of CTSD (A).', 'Soluble (diethylamine-extracted) forms of both A species were also significantly increased in KO + mice (B).', 'In the context of hAPP overexpression, it was evident unambiguously that human A does indeed accumulate in the brains of KO+, but not WT + or HET + mice, in the form of intense intracellular A immunoreactivity presenting in a punctate, predominantly perinuclear pattern (C).', ', amyloid plaques) in KO + mice (C).', 'Consistent with other reports [9, 18], no significant differences in insoluble (D) or soluble (', 'Similarly, we detected no qualitative differences in A plaque morphology (F) or quantitative differences in amyloid plaque number or A -positive area (Sup ', 'ELISA-based quantification of endogenous, murine A levels likewise revealed no differences between 10-month-old HET and WT mice, neither insoluble (Sup D) nor soluble (Sup ', 'Analysis of A accumulation in CatD-deficient hAPP transgenic mice.', 'Note the lack of differences in plaque size, number or morphology, as confirmed by morphometric quantification (Sup A-C).']","Figure 1 Analysis of A accumulation in CatD-deficient hAPP transgenic mice. Relative levels of insoluble ( ) and soluble ( ) A40 (left) and A42 (right) in 3-week-old hAPP transgenic mice with two (WT+), one (HET+), or no (KO+) functional copies of determined by ELISA specific for human A. Note the prominent increases in insoluble A levels in KO+ animals relative to both WT+ and HET+ controls. Data are mean SEM of 6 mice per genotype. ( ) Representative images of pan-A immunoreactivity in WT+ (left) and KO+ (right) brain. Note the prominant accumulation of A in KO+ mice, which occurs exclusively intracellularly (arrowheads), in the absence of extracellular deposits. Scale bars represent 50 m for the main images, and 20 m for the insets. ( ) Relative levels of insoluble ( ) and soluble ( ) A40 (left) and A42 (right) in 6- to 10-month-old hAPP transgenic mice with two (WT+) or one (HET+) functional copies of determined by ELISA. No significant differences are evident between genotypes. Data are mean SEM of 68 mice per group. ( ) Representative images of A plaques in WT+ (left) and HET+ (right) cortex. Note the lack of differences in plaque size, number or morphology, as confirmed by morphometric quantification (Sup Fig. 1A-C). * 0.05; ** 0.01; *** <0,001; **** <0.0001; ns=not significant.",yes
PMC7498635,Figure_4,oa_package/bb/72/PMC7498635.tar.gz,[],Supplemental Figure1 Pulsed-wave Doppler of the mitral inflow (left panel) and tissue Doppler of the septal mitral annulus (right panel).,yes
PMC5094822,Figure_1,oa_package/01/f5/PMC5094822.tar.gz,"['In MRI,\nloose bodies show hypointense signals on T1-weighted sequences and hyperintense\nsignals on T2-weighted sequences, due to the high amount of water in cartilage\ntissue, with foci of signal reduction, corresponding to calcifications ().', '\nSynovial chondromatosis.']","Figure 1 Synovial chondromatosis. Sagittal proton density (PD)-weighted MRI scanswith fat suppression. Multiple intra-articular loose bodies located inthe suprapatellar recess (arrow) and posterior recess (asterisk), wherea popliteal cyst (Baker's cyst) can be seen.",yes
PMC11414499,Figure_3,oa_package/fc/4e/PMC11414499.tar.gz,"['Fibrotic areas were identified in the region of the middle meatus and in the middle and superior ethmoid bone, adjacent to the perpendicular lamina of the ethmoid bone (the most central portions of the polypoid tissue) in all cases, while the edematous areas were found in the peripheral portions of the polyps closer to the floor of the nasal fossa and in a more anterior or posterior position in the nasal cavities ().', 'Illustration of the MRI difference between the peripheral and central regions of the nasal polyposis in T2-weighted image.', 'Differentiation between the central portion of the middle meatus and the peripheral portion of polypoid tissue of overall ADCsThe mean ADC values of the peripheral region were significantly higher than those of the central region of the nasal polyposis.']",Figure 3 Illustration of the MRI difference between the peripheral and central regions of the nasal polyposis in T2-weighted image. (AC) Hyperintense on T2-weighted in the peripheral portion (yellow arrow) contrasting with areas of hypointense on T2-weighted in the central portion (white arrow).,yes
PMC6607360,Figure_8,oa_package/e2/1c/PMC6607360.tar.gz,"['In CO-related encephalopathy, there are three general patterns of involvement: PVWM (most common), basal ganglial, and hippocampal (least common), usually with ADC reduction in the acute phase () [6,7,37,38].', 'A 33 year old male with ATL from carbon monoxide (CO) poisoning, in which the symptoms and imaging findings later improved, having severely elevated serum CO levels.', 'Ethanol (EtOH) neurotoxicity uncommonly causes ATL, albeit rare, as hippocampal edema (from withdrawal seizures) is the most common MRI finding of EtOH neurotoxicity; less common findings are Wernicke encephalopathy from thiamine deficiency, and rarely ATL [39].']","Fig. 8 A 33year old male with ATL from carbon monoxide (CO) poisoning, in which the symptoms and imaging findings later improved, having severely elevated serum CO levels. : an MRI demonstrates reduced diffusion in bilateral PVWM ( ) on DWI ( ) and ADC map ( ), these abnormalities had mostly resolved 9 months later ( ), as shown on DWI ( ) and with mild resultant diffuse cerebral atrophy on FLAIR ( ).",yes
PMC10591053,Figure_6,oa_package/6c/22/PMC10591053.tar.gz,"['5"">Survival analysis using MesoScoresSurvival analysis using Kaplan-Meier plots are shown in .', 'This difference in survival can be observed in the Kaplan Meier plot (A), where the predicted non-sarcomatoid curve in orange is less steep than the blue predicted sarcomatoid curve.', ' 6Survival prediction using MesoScore(A) Kaplan-Meier curves for all data.', '5"" ref-type=""sec"">Survival analysis using MesoScores and in .']","Figure5 Illustrations of morphological differences between predicted subtypes Top: examples of cells scored most and least highly by the model, plotted as a 2D UMAP reduction of principal components calculated on both high- and low-scoring cells. For each cluster, the mean of the cells is displayed, together with individual example cells. Clusters AE, on the left, are predicted to be sarcomatoid and demonstrate a more spindle-like morphology, grouped together in size and relative spindle cell characteristics in each cluster as shown by example cells on the right. Similarly, the non-sarcomatoid predicted cells also show clustering into 5 groups, FJ. Bottom: morphological heterogeneity of mesothelioma tumors independent of model prediction. (A) Distribution of average morphology across tumor types. All measurements are normalized to the data average and standard deviation. (B) Heterogeneity of cell morphology across different mesothelioma tumor types based on standard deviation (SD) of scored single-cell data. (C and D) Morphological heterogeneity based on ground-truth labels (C)and predicted labels (D).",yes
PMC9678881,Figure_5,oa_package/26/f9/PMC9678881.tar.gz,"['5A).', '5B, C).', '5D).', '5E).', 'Aberrant autophagic flux in SMS patient-derived cells.', 'Accumulation of vacuoles and swollen mitochondria in SMS cellsWe subsequently performed an ultrastructural electron microscopy analysis of SMS and CTR cells.', '5), we found that the level of mitophagy was significantly lower in the two SMS cell lines than in CTR cells (']","Fig. 5 Aberrant autophagic flux in SMS patient-derived cells. Immunofluorescence analysis of LC3 levels showing the accumulation of LC3 in SMS cells. Left panel: Representative images of =3 biological replicates. Right panel: Quantification of LC3 fluorescence intensity in 150 cells from three biological replicates. , Western blot analysis of the LC3 and p62 levels in SMS (RAI1-del1 and RAI1-S399P40fx) cells compared with sibling and CTR (sibling and CTR1) cells. Top panels: Representative images from three biological replicates. Bottom panels: Quantification of Western blots. Western blotting analysis of the LC3 and p62 levels in SMS (RAI1-del1 and RAI1-S399P40fx) cells compared with sibling and CTR (sibling and CTR1) cells treated with either vehicle or chloroquine (CQ, 40mM, 24h). Top panels: Representative images from three biological replicates. Bottom panels: Quantification of Western blots. LysoTracker staining analysis showing lysosomal accumulation in four SMS and four CTR cell lines. Left panel: Representative images of =3 biological replicates. Right panel: Quantification of LysoTracker fluorescence intensity in 100 cells from three biological replicates. Graphs: meanSEM, one-way ANOVA+NewmanKeuls post hoc test, * <0.05, ** <0.01, *** <0.001.",yes
PMC9985907,Figure_4,oa_package/f1/0b/PMC9985907.tar.gz,"['The histopathology pictures, epidermal hyperkeratosis, acanthosis, and focal regular elongation of rete ridges with clubbing.']","Figure 4 The histopathology pictures, epidermal hyperkeratosis, acanthosis, and focal regular elongation of rete ridges with clubbing. Increased basal layer pigmentation is noticed. The dermis shows focal areas with pigment incontinence.",yes
PMC10337884,Figure_7,oa_package/08/94/PMC10337884.tar.gz,[],"Figure7 GATA3 is required for normal Bhlhe40 and Egr2 expression at all stages. The expression of Egr2 and Bhlhe40 within -sufficient ( ) and -deficient (Cre-ERT2- ) CNS-infiltrating CD3 CD4 CD44 Foxp3 T effector cells from co-transfer EAE recipient mice treated with tamoxifen (cell transfer d0). Representative Egr2 and RORt staining and summary statistics amongst tamoxifen treated CNS-infiltrating CD4 CD44 Foxp3 and Cre-ERT2- cells. Representative Bhlhe40 and RORt staining and summary statistics amongst tamoxifen treated CD4 CD44 Foxp3 and Cre-ERT2- cells. n=4 mice/condition from two independent experiments. The expression of Egr2 and Bhlhe40 within -sufficient (Vehicle, d0) or -deficient (Tamoxifen, d0) day 6 dLN CD3 CD4 CD44 Foxp3 T effector cells from MOG /CFA-immunized Cre-ERT2- mice. Representative Egr2 and RORt staining and the corresponding summary statistics from vehicle control or tamoxifen treated d6 dLN Cre-ERT2- CD4 T effector cells. Representative d6 dLN CD4 T effector Bhlhe40 and RORt staining and the corresponding summary statistics from immunized and vehicle or tamoxifen treated Cre-ERT2- mice. n=4 mice/condition from two independent experiments. For statistical comparisons, unpaired students T tests were used. Significance levels are denoted as follows: *p <0.05; **p <0.01; ***p <0.001.",yes
PMC6837781,Figure_4,oa_package/6b/e3/PMC6837781.tar.gz,"['However, the fistula was still filling due to high flow () and the procedure was abandoned because of incomplete embolisation with plans made for a second intervention.']",FIGURE 4 Angiogram after the first-coil embolisation demonstrating the first micro coil that migrated to the venous side because of high flow. The ectatic segment of the feeding artery was packed with coils but despite that there was still filling of the arteriovenous malformation because of high flow.,yes
PMC11480867,Figure_3,oa_package/80/76/PMC11480867.tar.gz,"['The right colon remained dilated, yet no apple core sign was observed from the transverse colon to the hepatic flexure (A).', 'The contrast medium did not flow into the descending colon (B).', '.']","Figure 3. Enterography using a transanal drainage tube showed no apple core signs from the transverse colon to the hepatic flexure ( ), and no contrast medium flowed into the descending colon ( ).",yes
PMC4962319,Figure_1,oa_package/09/48/PMC4962319.tar.gz,"['Western blot analysis showed that these constructs are expressed at comparable levels in HEK293T cells (A).', 'When transiently transfected, wild-type Tau constructs did not promote SG formation and associate with SGs, as shown by co-immunostaining with Tau-5 and TIA-1 antibodies (B).', 'Cells transfected with TauE14 showed a few puncta that co-stained with Tau and TIA-1, while cells expressing Tau-GLuc treated with arsenite, a classical inducer of SGs, showed a prominent stress granule response and also some recruitment of wildtype Tau to SGs (B,C).', 'In further experiments, we used salubrinal, an inhibitor of eIF2 dephosphorylation23, to promote SG formation with less toxicity24 (D).', 'On the other hand, Tau transfection itself resulted in almost 50% decreased viability with both the wildtype and the TauE14 forms, while further salubrinal treatment did not further exacerbate Tau toxicity, suggesting that SG formation does not affect viability in Tau-transfected cells (D).', 'When cell viability was assessed by LDH release measurement, neither salubrinal or arsenite treatments increased LDH release, suggesting that the cell membrane remained intact in the experimental conditions used in this study (E).', 'As shown in F, when coexpressed wildtype Tau shows only weak interaction with TIA-1 while TauE14 shows more than 6-fold higher interaction signal.', 'While in wells containing cells expressing TIA-1-GLuc1 and GSK3 -GLuc2 the signal was nearly undetectable, there was clear luminescence signal generated in wells containing cells expressing TIA-1-GLuc1/Tau-GLuc2 and TIA-1-GLuc1/TauE14-GLuc2 combinations (G).', 'Because of the differences seen between wildtype Tau and TauE14 in the transfection and the coplating conditions (F G), we hypothesized that wildtype Tau could undergo posttranslational modification (such as phosphorylation, as suggested by the phosphomimetic form of Tau) that would enhance its ability to interact with proteins involved in SG formation.', 'Transfected and internalized extracellular Tau differ in their ability to associate with stress granules.']","Figure 1 Transfected and internalized extracellular Tau differ in their ability to associate with stress granules. ( ) Expression of non-tagged Tau, Tau-GLuc1/2 and TauE14-GLuc1/2 in HEK293T cells as detected by Western blot. The blot picture was cropped from a larger original image, maintaining all the stained bands. ( ) HEK293T cells transiently transfected with the above-mentioned constructs and stained with Tau-5 (green) and TIA-1 (red) antibodies. Arsenite (0.5mM for 30min) was used as a positive control for induction of stress granules. ( ) Quantitative analysis of stress granule formation. Stress granule-positive cells were counted among the Tau-transfected cells. Arsenite treatment promoted stress granule-formation in all cells while only some Tau-transfected, and more efficiently TauE14-transfected, cells contained stress-granules (n=3). ( ) Resazurin-based cell viability assay with HEK293T cells transiently transfected with the Tau constructs. Salubrinal and arsenite were used as positive controls for stress granule induction (n=4). ( ) LDH release assay in HEK293T cells transfected with the Tau constructs and treated with salubrinal and arsenite as positive controls (n=4). Relative LDH release was calculated from the ratio of LDH in the media and total LDH (from media and the cells). ( ) Protein-fragment complementation assay (PCA) in HEK293T cells transiently transfected with TIA-1-GLuc1, Tau-GLuc2 and TauE14-GLuc2 (n=3). ( ) PCA-based coplating experiment to study cell-to-cell transfer of Tau. Batches of HEK293T cells were separately transfected with a single PCA construct (TIA-1-GLuc1, Tau-GLuc2, TauE14-GLuc2 or GSK3-GLuc2 as a control), followed by replating the cells in various combinations 24h post-transfection (n=3). Scalebar=10m; average +/SEM is shown; ***p<0.001; **p<0.01; *p<0.05.",yes
PMC6368538,Figure_2,oa_package/11/ca/PMC6368538.tar.gz,"[' 2A,B).', 'HSPCs frequency in CHD patients.', 'Table 2Characteristics of CHD patients by coronary stenosis severity.', ' 2C,D).', ' 2E,F).', ' 2G I).', ' 2J,K) but not in non-CHD participants (p 0.']","Figure 2 HSPCs frequency in CHD patients. Mild coronary stenosis <50% ( ) and severe coronary stenosis 70% ( ), as detected by coronary angiography. ( ) The percentage of CD11b+ cells in freshly isolated HSPCs ( ). ( ) HSPCs were plated on Methylcelluose cultures for 14 days. Cells were harvested and stained with anti-human CD11b. The percentage of CD11b+ cells was shown. ( ) HSPCs frequency in subjects with and without CHD. ( ) HSPCs frequency in CAD with different extents of stenosis. HSPCs frequencies in e and fare expressed as mean and 95% CI. ( ) Representative FACS plot of HSPCs analysis. Among all samples, at least8728 (95%CI, 578311674) CD34+ cells were obtained in the analysis. ( , ) The correlation curves of HSPCs frequency and white blood cell count or neutrophil count in CHD patients, respectively.",yes
PMC4053636,Figure_1,oa_package/b2/bd/PMC4053636.tar.gz,[],"Fig.1 A dedicated segmentation pipeline was developed by combiningVBM8, SPM8 ( ), Freesurfer ( ) and BrainVISA ( ) in order to cumulate their strength. It results in a set of sulcal pieces, each sulcal part being associated with a set of shape descriptors. The 4 steps are detailed in the text and supplementary materials and the sulcal attributes are illustrated inpanel 4: sulcal pieces that are colored following a) anatomical denomination, b) mean depth, c) maximum depth and d) area of the folds.",yes
PMC10400774,Figure_5,oa_package/de/68/PMC10400774.tar.gz,"['Our study and several previous case reports indicated that some demyelinating lesions found in patients with MOGAD showed MOG-dominant myelin loss especially in the early-stage () (33, 37, 42, 46), and some myelin-laden macrophages localized at the perivascular space showed MOG-dominant phagocytosis (33).', 'Characteristics of demyelinating lesions in MOGAD.', 'In the demyelinating lesions in MOGAD, astrocytes are essentially activated and AQP4 is also strongly stained on immunohistochemistry images (), although two cases of partially decreased AQP4 expression in MOGAD with tumefactive brain lesions have been reported (Table 2) (44).']","Figure 5 Characteristics of demyelinating lesions in MOGAD. Loss of MOG staining was more evident than MAG staining. Oligodendrocytes were well preserved in the demyelinating lesion. Axonal enlargement was present, suggesting neuroaxonal alteration but axonal staining was relatively preserved compared to demyelination. Activated astrocytes with dense AQP4 staining were observed. MOG, MAG, TPPP, NF, GFAP, AQP4. AQP4, aquaporin 4; GFAP, glial fibrillary acidic protein; MAG, myelin associated glycoprotein; MBP, myelin basic protein; MOG, myelin oligodendrocyte glycoprotein; MOGAD, MOG antibody-associated disease; NF, neurofilament; TPPP, tubulin polymerization promoting proteins.",yes
PMC8167394,Figure_8,oa_package/1a/4b/PMC8167394.tar.gz,"['If Ewing sarcoma represents the prototype of round cell sarcoma, CIC sarcomas always exhibits focal pleomorphism, and at times epithelioid morphology can predominate (A).', 'BCOR sarcomas (despite being allocated to the round cell sarcoma family) more often tend to exhibit a spindled morphology (B).', 'NFATC2 sarcoma may exhibit remarkable epithelioid features (C), and PATZ1 sarcomas are composed of a rather undifferentiated round to ovoid cell population (', 'A.', 'B.', 'C.', 'D.']",Figure 4. . In this example an epithelioid atypical neoplastic cell population predominates.,yes
PMC5949322,Figure_7,oa_package/5e/b8/PMC5949322.tar.gz,['Schematic diagram showing the invariant natural killer T (iNKT) cell microbiota neutrophil axis in regulation of dextran sodium sulfate (DSS)-induced intestinal inflammation.'],"Figure 7 Schematic diagram showing the invariant natural killer T (iNKT) cellmicrobiotaneutrophil axis in regulation of dextran sodium sulfate (DSS)-induced intestinal inflammation. (1) Continual administration of 2% DSS in wild-type (WT) mice results in epithelial damage and allows translocation of inherently pro-colitogenic gut bacteria. This results in local colonic inflammation, production of IL-1, CXCL1, and CXCL2, and the recruitment of immune cells, including neutrophils. (2) Antigen presentation of the translocated bacteria to iNKT cells acts to suppress exacerbated recruitment of neutrophils. (3) Once recruited, neutrophils produce IL-1, CXCL1, and CXCL2, which function in an autocrine manner for further recruitment. (4) In the highly inflammatory environment, neutrophils polarize toward a pro-inflammatory phenotype. (5) This inflammatory phenotype, in combination with low production of bactericidal iNOS, is detrimental and results in increased intestinal inflammation. (6) In mice, there is translocation of anti-colitogenic gut bacteria. (7) Without iNKT cells, there is earlier and more pronounced recruitment of neutrophils to the colon. (8) The recruitment is likely sustained due to the autocrine functions of IL-1, CXCL1, and CXCL2. (9) The presence of anti-colitogenic bacteria supports neutrophils to function toward an anti-inflammatory phenotype. (10) Increased production of iNOS by neutrophils enables dual bactericidal and immune-modulatory functions, thereby resulting in lower disease pathology. (Stock images sourced from Servier Medical Art; Creative Commons)",yes
PMC11469284,Figure_1,oa_package/21/f3/PMC11469284.tar.gz,"['This methodology allowed for the consistent and unbiased determination of where to measure the RNFL ().', '.', 'EDA+/+ mice had significantly elevated IOP compared to C57BL/6J controls that persisted through 22 months of age (A): at 12 months (n = 12 to 14 eyes/strain), C57BL/6J 13.', 'EDA+/+ mice had significant RGC loss compared to C57BL/6J controls at both 12 and 22 months (B, representative images Figs.', 'EDA+/+ mice at both 12 months and 22 months of age had a significant decrease in RNFL thickness compared to age-matched C57BL/6J controls (H): at 12 months (n = 3 to 5 eyes/strain), C57BL/6J 62.', 'EDA+/+ mice (A), leading to significant RGC loss (', '1B) and decreases in RNFL thickness (H).']","Figure 1. B6.EDA mice exhibited glaucoma phenotypes at 12 months and 22 months of age. ( ) IOP was significantly elevated in B6.EDA mice at 12 months ( = 12 to 14 eyes/strain) and 22 months ( = 16 to 18 eyes/strain) compared to age-matched C57BL/6J controls. Significance was determined by Student's -test at each time point. ( ) B6.EDA mice ( = 3 to 7 eyes/strain) showed an 20% loss of RGCs at both 12 months and 22 months of age compared to C57BL/6J controls, as quantified by RBPMS staining of retinal flatmounts. Significance was determined by Student's -test at each time point. ( ) Representative images of RBPMS staining at 12 months and 22 months for B6.EDA mice and age-matched C57BL/6J controls. ( ) The location for measurements of RNFL thickness was standardized by drawing a line from the bottom of the inner nuclear layer ( ) to the top of the outer nuclear layer ( ) and extended to the top of the RNFL ( ). Measurements were then taken for the RNFL thickness only ( ). ( ) B6.EDA mice exhibited significant thinning of the RNFL at both 12 months and 22 months of age compared to age-matched C57BL/6J controls ( = 3 to 5 eyes/strain). RNFL thickness was measured using ImageJ, and significance was determined by Student's test at each time point. * < 0.05, ** < 0.01, **** < 0.0001.",yes
PMC10879047,Figure_4,oa_package/3c/9f/PMC10879047.tar.gz,[' 4US post PTI revealing patent PEG balloon and no hemorrhageWe suggest a 2500 International Units (IU) dose for all patients.'],Fig.4 US post PTI revealing patent PEG balloon and no hemorrhage,yes
PMC4469142,Figure_2,oa_package/7a/f3/PMC4469142.tar.gz,[],"Figure 2 Lung autopsy specimen showing a diffuse alveolar damage pattern with prominent hyaline membranes in the alveoli (hematoxylin and eosin stain, 40).",yes
PMC10570503,Figure_6,oa_package/8d/e8/PMC10570503.tar.gz,[],"Figure6 ROC curves. Training sets at 1, 3, and 5 years. Validation sets at 1, 3, and 5 years.",yes
PMC9698920,Figure_2,oa_package/1d/29/PMC9698920.tar.gz,[],Figure2 Chest computed tomography scan revealed a large soft tissue mass in the left breast with uniform density.,yes
PMC5686970,Figure_8,oa_package/dd/9f/PMC5686970.tar.gz,"['In male patients (9 cases), expression of PR receptors positive was nearly equal to PR receptor negative cases [].', 'Table 4Tukey-Kramer multiple comparison test of Ki-67 expressionExpression of PR receptors in male and female cases of meningiomaDiscussionTumor of CNS represents 1.']",Figure 8 Expression of PR receptors in male and female cases of meningioma,yes
PMC3977525,Figure_1,oa_package/04/f7/PMC3977525.tar.gz,['0-0018436924109602(a) Vacuolar degeneration of hepatocytes; (b) E.'],Figure 1 (a) Vacuolar degeneration of hepatocytes; (b) spores in intestinal lumen; (c) hepatic granuloma; (d) glial nodule in the cerebral cortex. All HE; 400x.,yes
PMC6786501,Figure_1,oa_package/eb/0f/PMC6786501.tar.gz,"['1).', 'Axial cut at T3 level on CT scan (a), midsagittal cut on CT scan (b), as well as T2 weighted sagittal MRI scan (c) images of the thoracic spine of a 70-year-old male patient (case 1) showing ossification of the thoracic posterior longitudinal ligament behind T2 to T4 levels with anterior compression of the cord and associated signal intensity changes at the same levelSurgical detailsThe patient was positioned prone with appropriate padding of all bony prominences and ensuring that the spine was aligned with the hips extended.']","Fig. 1 Axial cut at T3 level on CT scan ( ), midsagittal cut on CT scan ( ), as well as T2 weighted sagittal MRI scan ( ) images of the thoracic spine of a 70-year-old male patient (case 1) showing ossification of the thoracic posterior longitudinal ligament behind T2 to T4 levels with anterior compression of the cord and associated signal intensity changes at the same level",yes
PMC2478665,Figure_2,oa_package/82/6f/PMC2478665.tar.gz,['Intraoperatively identified appendiceal mucocele.'],Figure 2 . The mucocele was in the antecaecal location and on macroscopic assessment showed a smooth serosal surface without peritoneal implants.,yes
PMC11191378,Figure_3,oa_package/5e/3d/PMC11191378.tar.gz,['T2-weighted TSE sagittal MRI image of the normal cis-femaleOriginal MRI image of a cis-female patient taken from the archive.'],Figure 3 T2-weighted TSE sagittal MRI image of the normal cis-female Original MRI image of a cis-female patient taken from the archive. 1 - Neovaginal depth; 2 - Inlet of the pelvis (IOP); 3 - Inferior pelvic aperture (IPA); - Alpha angle,yes
PMC9628656,Figure_5,oa_package/44/61/PMC9628656.tar.gz,"['Subsequently, a successful angioembolisation of the right second lumbar artery was performed, which fully resolved the symptoms (as seen in figure 5).', 'Snapshot showing embolisation of lumbar arterial bleed (as indicated by the black arrow).']",Figure 5 Snapshot showing embolisation of lumbar arterial bleed (as indicated by the black arrow).,yes
PMC9520417,Figure_1,oa_package/1f/7d/PMC9520417.tar.gz,"['The plain radiographs of the pelvis reveals multiple high-density opacities consistent with soft-tissue calcifications opposite the left greater trochanter without osseous lesion (yellow arrows).', 'MRI of the pelvis on T1-weighted axial and coronal MRI, showing a lobulated mass of the right hip, well-delimited, and infiltrating the muscles, in hyposignal T1 (red circle).']",Fig. 1 The plain radiographs of the pelvis reveals multiple high-density opacities consistent with soft-tissue calcifications opposite the left greater trochanter without osseous lesion (yellow arrows).,yes
PMC2929538,Figure_3,oa_package/37/eb/PMC2929538.tar.gz,"['Magnification can be increased by means of a double mouse click on the image [].', 'More information including microscopic findings, ancillary study result, final diagnosis, and discussion will display when the diagnosis-button is clickedA whole slide image is displayed via spectrum webscope in a pop-up window.']",Figure 3 A whole slide image is displayed via spectrum webscope in a pop-up window. A tool bar (shown at the bottom of the screen) can be used to change the power and to move the display field,yes
PMC8559565,Figure_5,oa_package/df/d0/PMC8559565.tar.gz,"['This can be explained as the abraded tendon is compressed against the bone as it wraps around the radius in pronation ().', '200145"" ref-type=""bibr"">24(A) The biceps provocation test is a two-part test.']","Fig. 5 (A) The biceps provocation test is a two-part test. The patient is standing, with the elbow supported by the examiner and flexed to 70 degrees. The examiners hands are placed on the patients forearm and the patient is asked to flex the elbow against resistance with the forearm supinated (ABTs). The forearm is then pronated, and the test is repeated (ABTp). Care is taken not to place the hands on the hand or wrist, as resisted wrist flexion or extension might elicit pain in other elbow pathologies. (B) On the left, the position of the biceps tendon with the forearm supinated (tear in green). On the right, the position of the biceps tendon with the forearm pronated (tear in red). As the distal biceps tendon wraps around the radial tuberosity when the arm is pronated, the tendon is stretched and compressed when the biceps is activated. . Copyright MoRe Foundation.",yes
PMC10100625,Figure_5,oa_package/80/2e/PMC10100625.tar.gz,"[' 5).', 'Illustration reproduced from TACTICS registry demonstrating OCT (Optical Coherence Tomography) images of the different causes of ACS (Acute Coronary Syndromes) using OCT-defined morphological assessment.', 'Reproduced with kind permission from the Journal of American College of Cardiology [43]Antiplatelet therapyStrategies to shorten DAPT duration post-PCI in high bleeding risk patients continue to be evaluated.']",Fig. 5 Illustration reproduced from TACTICS registry demonstrating OCT (Optical Coherence Tomography) images of the different causes of ACS (Acute Coronary Syndromes) using OCT-defined morphological assessment. Reproduced with kind permission from the Journal of American College of Cardiology [ ],yes
PMC6699281,Figure_2,oa_package/5c/3b/PMC6699281.tar.gz,"['This was performed by infiltrating the overlying skin with Marcaine, incising vertically at midline, and then dissecting around the cyst using both blunt and sharp dissection ().', '001""/>Cystic appearance of the neuroma.']",Figure 2 Cystic appearance of the neuroma.,yes
PMC3551781,Figure_6,oa_package/2e/b4/PMC3551781.tar.gz,['Origin of new granulosa cells from OSC during the prime reproductive period in adult human ovaries.'],"Figure 6 ) Panoramic view of ovarian surface and adjacent cortex. Dashed line indicates interface between TA and stroma of the ovarian cortex. osc and black arrow - ovarian stem cells; taf and black arrowhead - TA flap; white arrowhead - a lack of OSC above the TA; white arrow -bilaminar epithelial cord. ) Detail from (A) shows association of CK+ (brown color) fibroblasts (+fb,) with the TA flap surface (arrowhead), transition from mesenchymal to epithelial morphology (fb/se), and ovarian stem cells (osc, arched arrow). ) A parallel section to (B) showing numerous DR+ MDC (asterisks) in the TA flap. Note DR expression also in early OSC (arrow). ) Detail from (A) shows CK+ epithelial cord consisting of two layers of epithelial cells and lying between the ovarian cortex (ovc) and TA (ta). Note diminution of CK immunoexpression in TA fibroblasts (+/-fb). E) Epithelial cords (black arrows) fragmenting into granulosa cell nests (arrowheads in the upper ovarian cortex (uc). White arrow CK+ OSC associated with the TA with flap. ) Lower ovarian cortex (lc) with primordial follicles. Arrow indicates distance from the ovarian surface, dashed box indicates follicle shown in the inset. F29 indicates female age in years. Bar in (D), for (B-D). Panels A, B, D-F adapted from[ ], Antonin Bukovsky; panel C from[ ], Wiley-Liss, Inc. with permission.",yes
PMC10545134,Figure_1,oa_package/df/10/PMC10545134.tar.gz,['CT angiography of the chest reveals the incidental finding of the beaver tail liver as indicated by the arrows.'],Figure 1 CT angiography of the chest reveals the incidental finding of the beaver tail liver as indicated by the arrows.,yes
PMC9730148,Figure_1,oa_package/d9/24/PMC9730148.tar.gz,"[')Central IllustrationKey Wordsaccelerated idioventricular rhythmathletehypertrophic cardiomyopathysports cardiologyventricular rhythmAbbreviation and AcronymsAIVR, accelerated idioventricular rhythmCMR, cardiac magnetic resonanceECG, electrocardiogramHCM, hypertrophic cardiomyopathySCD, sudden cardiac deathHistory of PresentationBefore sports college admittance, a 19-year-old male athlete underwent sports physician led preparticipation screening.', 'A resting electrocardiogram (ECG) was performed, which revealed accelerated idioventricular rhythm (AIVR) alternating with sinus bradycardia, with normal PR and QRS conduction intervals ( 1).', ' 1Resting ElectrocardiogramResting electrocardiogram performed during routine preparticipation screening showing alternating sinus rhythm and accelerated idioventricular rhythm (AIVR).']",,yes
PMC9409160,Figure_5,oa_package/b2/f7/PMC9409160.tar.gz,"['NeuropathologyIn ETX-exposed sheep following a more protracted clinical course, bilaterally symmetrical foci of necrosis are sometimes found in certain brain regions of predilection, including the basal ganglia, thalamus, internal capsule, midbrain, medulla oblongata, and cerebellar peduncles (), this neuropathologic entity being termed FSE [20,76,77,78].', 'Focal symmetrical encephalomalacia (FSE) in a sheep showing bilaterally symmetrical necrotic foci in the corpus striatum, thalamus and cerebellar peduncles.']","Figure 5 Focal symmetrical encephalomalacia (FSE) in a sheep showing bilaterally symmetrical necrotic foci in the corpus striatum, thalamus and cerebellar peduncles. Photo courtesy of B. Hartley.",yes
PMC6905210,Figure_1,oa_package/c5/9f/PMC6905210.tar.gz,"['Integrating the data handled in this review, we suggest a model in which Alzheimer disease pathogenesis could be exacerbated by MeCP2 dysfunction ().', '3390/ijms2010250878SquillaroTAlessioNCipollaroMRenieriAGiordanoAGalderisiUPartial silencing of methyl cytosine protein binding 2 (MECP2) in mesenchymal stem cells induces senescence with an increase in damaged DNAFASEB J20102415936032006510579Van EschHMECP2 duplication syndromeMol Syndromol20122128362267939980ZeevBBYaronYSchanenNCWolfHBrandtNGinotNRett syndrome: clinical manifestations in males with MECP2 mutationsJ Child Neurol2002172041191356481WardCSHuangTWHerreraJASamacoRCPitcherMRHerronALoss of MeCP2 causes urological dysfunction and contributes to death by kidney failure in mouse models of Rett syndromePLoS One201611e01655502782899182SadeghMKEkmanMKrawczykKSvenssonDG ranssonODahanDDetrusor induction of miR-132/212 following bladder outlet obstruction: association with MeCP2 repression and cell viabilityPLoS One201510e01167842561789383KashyapMPoreSChancellorMYoshimuraNTyagiPBladder overactivity involves overexpression of MicroRNA 132 and nerve growth factorLife Sci20161679810427789288.']","Fig. 1. A schematic model depicting the impact of MeCP2 dysfunction in Alzheimer disease. 1-1) MeCP2 deficiency induces cellular senescence and upregulation of senescence-associated secretory phenotype (SASP) genes in glial cells (astrocytes and microglia). 1-2) Senescent glia secrete SASP molecules (cytokines, chemokines, proteases, and growth factors), which in turn directly induce neuroinflammation in neurons and give rise to reactive glia that induce excitotoxicity. 2-1) MeCP2 upregulation causes downregulation of the Alzheimer disease risk genes , and in neurons. 2-2) In conjunction, MeCP2 upregulation exacerbates tau pathology in neurons. 3) Taken together, MeCP2 dysfunction results in the neurodegeneration observed in Alzheimer disease. Red arrows indicate the effects exerted by MeCP2 deficiency and blue arrows indicate the effects resulting from MeCP2 upregulation.",yes
PMC5984788,Figure_7,oa_package/2b/d8/PMC5984788.tar.gz,"[' 7a and b); similar effect on paraspeckles was observed with short (4 h) treatment and with a higher enoxacin dose (Additional file 6: ', '7a and b).', '7a and b).', 'A small molecule enhancer of miRNA biogenesis stimulates paraspeckle assembly in neuroblastoma cells.', 'Scale bar, 10 mDiscussionNuclear bodies spatially organize and modulate various cellular processes [54].']","Fig. 7 A small molecule enhancer of miRNA biogenesis stimulates paraspeckle assembly in neuroblastoma cells. and SH-SY5Y cells were treated with enoxacin, riluzole, edaravone and HDAC inhibitors trichostatin A (TSA) and sodium butyrate (NaB), and paraspeckle assembly was assessed by NEAT1_2 RNA-FISH ( ) and qRT-PCR (n=46, ). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 (Kruskal-Wallis test with Dunns correction for multiple comparisons). Cells were harvested for analysis after 4h of TSA and NaB treatment (500nM and 2mM, respectively) and after 24h of enoxacin, riluzole and edaravone treatment (all 10M). Scale bar, 10m",yes
PMC5996926,Figure_4,oa_package/30/f6/PMC5996926.tar.gz,['CD8+ T cell differentiation is determined by IL-2 IL-2R signaling.'],"Figure 4 CD8 T cell differentiation is determined by IL-2IL-2R signaling. Expression of IL-2 in activated CD4 and CD8 T cells at day 5. Expression of IL-2 receptor alpha chain CD25 on day 4 after activation of CD8 T cells in the presence of different cytokines. Under anti-CD3/CD28, IL-6 and IL-6TGF- T 2 conditions, decreased expression of CD25 correlates with increased expression of IL-4 (upper panel); under IL-2+IL-6, IL-12, IL-2+IL-12 T 1 conditions, decreased expression of CD25 correlates with increased expression of IFN- (lower panel).",yes
PMC4080663,Figure_5,oa_package/3c/1c/PMC4080663.tar.gz,"['Moreover, the concordance with consensus diagnosis increased and variability reduced significantly with increasing experience of diagnosing lymphomas [].', 'Composite box plot showing the concordance of initial independent diagnosis and revised diagnosis with the consensus diagnosis for each group of pathologistsEffect of tissue fixation, age group and provision of additional information on revision of diagnosesAll biopsies were graded for quality of fixation and staining by morphological assessment on a semi quantitative scale of 1-3 with 3 being the best possible fixation and staining.']",Figure 5 Composite box plot showing the concordance of initial independent diagnosis and revised diagnosis with the consensus diagnosis for each group of pathologists,yes
PMC6737833,Figure_3,oa_package/87/be/PMC6737833.tar.gz,"['Frequencies of total CD8+ T cells are comparable in healthy donors and STAT3DN patients (D), however STAT3DN patients have an increase in na ve and a corresponding decrease in TCM, TEM, and TEMRA CD8+ T cells (s 3E, 4E,F) (73).', 'Despite normal frequencies of total B cells (F), they tended to be more immature as revealed by an increase in transitional and na ve B cells and a concurrent decrease in memory B cells (s 1, 3G) (31, 32, 74).', 'Interestingly, despite the severe reduction in memory B cells, those that do develop in STAT3DN patients have undergone Ig isotype switching, albeit with a trend toward IgG and away from IgA (H) (31, 74).', 'Identification of immune cell populations in STAT3DN patients.', 'Similarly, ZNF341-deficient and STAT3DN patients had increased proportions of memory CD4+ T cells expressing the characteristic Th2 cytokines IL-4 and IL-13 (s 5C,D) (40), consistent with the finding of increased CD4+CD45RA CXCR5 CCR6 CXCR3 memory cells (C), as well as the hyper-IgE phenotype and Th2-associated pathologies, in these individuals (40, 95, 96).']","Figure 3 Identification of immune cell populations in STAT3 patients. Total CD4 T cells, nave, central memory (T ), effector memory (T ) CD4 T cells, regulatory T cells (Treg) T follicular helper (Tfh) cells, Th1, Th2, Th17 CD4 T cells, total CD8 T cells, nave, T , T , and revertant memory (T ) CD8 T cells, total B cells, transitional, naive and memory B cells, IgM, IgG and IgA memory B cells, NK cells, NKT cells, T cells and mucosal associated invariant T (MAIT) cells were identified in normal donors (N) and STAT3 patients (S). Each point represents a different sample and horizontal line represents the average; statistics were performed in Prism using -test ( < 0.05; < 0.01; < 0.001; < 0.0001). These data are compiled from findings previously reported in the following publications: ( , , , , ).",yes
PMC3785031,Figure_1,oa_package/6f/de/PMC3785031.tar.gz,"['CNPV (isolated from a canary), penguinpox virus (PEPV; from a penguin) and Pi2 (from a racing pigeon) caused no obvious membrane thickening, but the pock lesions produced by these viruses each differed in colour, size and density ().', 'CNPV infection resulted in small, yellow pocks () and PEPV pocks were very small, flat and white in colour.', 'However, the pocks resulting from Pi2 infection were large, raised, round and white with pink centres, possibly suggesting the presence of haemorrhage ().', 'RP2 (from a rock pigeon), LD2 (from a laughing dove) and Pi5 (from a racing pigeon) caused slight thickening of the CAM ().', 'RP2 and Pi5 presented white pocks that were variable in size, with some pocks having slightly haemorrhagic centres ().', 'FeP2 (from a feral pigeon), LD1 (from a laughing dove) and flamingopox virus (FGPV; from a flamingo) displayed a substantial amount of membrane thickening ().', 'FeP1 (from a feral pigeon), RP1 (from a rock pigeon) and Pi1 (from a racing pigeon) caused such extreme membrane thickening that individual pocks were not visible ().', '.', 'The histopathology of these virally infected CAMs revealed significant differences ( and Table 2).', 'Although viruses that caused severe macroscopic proliferation of the CAM were noted to have extensive mesodermal hyperplasia and less epidermal hyperplasia (see ), a more detailed histological analysis showed all viruses to be different from one another (Table 2).', ', 1972) (b).', 'The viruses FeP2, Pi5, LD2 and Pi2 exhibited pronounced immune infiltration, and angiogenesis was seen in the mesoderm (b).', 'The morphological and histological differences observed among APVs in this study (, Tables 1 and 2) could be attributed to the absence or presence of specific immunomodulatory gene products, which may influence inflammation.']","Fig. 1. Macroscopic and histological comparison of uninfected and infected CAMs of embryonated chicken eggs. Viruses (CNPV, PEPV, Pi2, FGPV, RP2, LD2, Pi5, FeP2, LD1, FeP1, RP1, and Pi1; see for abbreviations) (10 p.f.u.) were inoculated onto the CAMs of 1011-day-old embryonated chicken eggs. (a) Differences in pock morphology and degree of inflammation of the CAM tissue. Other observations are given in . Magnification 10, H&E stain. Bar, 200 m. (b) High-magnification comparison of an uninfected CAM and CAMs infected with 10 p.f.u. PEPV, FeP2 and RP1. Magnification 40, H&E stain. Bar, 50 m.",yes
PMC7544539,Figure_3,oa_package/f8/d9/PMC7544539.tar.gz,[],"Figure3 ECGi systems usually consist of a vest embedded with 50 to approximately 300 electrodes (depending on the manufacturer) that is fitted to the patients torso, which allows the acquisition of electrograms ( ). Patients with the vest still in position will need to undergo a thoracic CT scan to determine the precise anatomic relation between the cardiac geometry and the torso electrodes ( ). The ECGi system combines each data set obtained from the vest and CT scan, and an activation waveform map of both ventricles epicardial surface is generated and combined to construct 3D epicardial isochrone maps ( ). CT, computed tomography; ECGi, electrocardiographic imaging.",yes
PMC10657827,Figure_7,oa_package/5f/95/PMC10657827.tar.gz,"['7), cytoplasmic aggregates of TDP-43 were observed (', '7A), and whilst most nuclei stained robustly for TDP-43, some nuclei stained faintly for TDP-43, indicative of nuclear depletion (', '7B).', '7C).', '7D).', 'Mutant VCP patient fibroblasts exhibit pathology ameliorated by arimoclomol.', '001 (two-way ANOVA, n=3 controls, n=4 patients)Furthermore, in untreated mVCP fibroblasts, we also observed a significant increase in the number of nuclei with an abnormal morphology, consisting of herniations and fragmentation of nuclei leading to the generation of micronuclei (', '7E).', '7F).']","Fig. 7 Mutant VCP patient fibroblasts exhibit pathology ameliorated by arimoclomol. Expression of TDP-43 in untreated mVCP fibroblast cultures show ( TDP-43- positive aggregates or ( ) depletion of nuclear TDP-43 (indicated by white arrows). ( ) Control, and ( ) arimoclomol-treated mVCP cultures show no abnormal TDP-43 expression. Scale bar = 20 m. ( ) DAPI-labelled fluorescent images of abnormal nuclear morphology observed in mVCP patient fibroblasts showing nuclear herniation and nuclear fragmentation generating micronuclei. ( ) Quantification of disrupted nuclei in fibroblasts treated with increasing concentrations of arimoclomol, ** <0.01, *** <0.001 (two-way ANOVA, =3 controls, =4 patients)",yes
PMC8864204,Figure_1,oa_package/1d/33/PMC8864204.tar.gz,"['The presence of the MEV canal was noted and classified according to its size: less than 2 mm, between 2 and 5 mm, and more than 5 mm ().', 'Neurointervention20161192827621945Table 1The frequency of anatomical variationsTable 2Mean ages of patients with right and left MEV canalsTable 3Frequency distribution of MEV canals according to genderTable 4Frequencies and percentages of the right MEV according to the presence of JB and sigmoid sulcus variationsTable 5Frequencies and percentages of the left MEV according to the presence of JB and sigmoid sulcus variationsTable 6Frequencies and percentages of right and left MEVs by venous dominanceTemporal CT axial reformatted image shows the left mastoid emissary vein canal (arrow).']",Figure 1 Temporal CT axial reformatted image shows the left mastoid emissary vein canal (arrow). The diameter of the canal is 2.5 mm CT: Computed tomography,yes
PMC11550100,Figure_1,oa_package/6f/b1/PMC11550100.tar.gz,"['Clinical imageBlack arrows show blackish-brown papules on the forehead and right malar regionBased on the clinical presentation, a differential diagnosis of sebaceous adenoma and verruca vulgaris was considered.']",Figure 1 Clinical image Black arrows show blackish-brown papules on the forehead and right malar region,yes
PMC10719075,Figure_6,oa_package/b5/f4/PMC10719075.tar.gz,"['Hematoxylin and eosin stain; (A) x100; (B) x200, and (C) x400 magnificationLight microscopy photographs of the tumor showing (A) nuclear positivity to ERG, (B) cell membrane positivity to CD31, and (C) cell membrane positivity to CD34 (x200 immunohistochemistry stain)ERG: electroretinogramDiscussionAs already mentioned, angiosarcoma is a primary mesenchymal neoplasm of endothelial cell origin with anastomosing vascular channels, and the real reason and trigger behind the formation of angiosarcoma still not known [1].']","Figure 6 Light microscopy photographs of the tumor showing (A) nuclear positivity to ERG, (B) cell membrane positivity to CD31, and (C) cell membrane positivity to CD34 (x200 immunohistochemistry stain) ERG: electroretinogram",yes
PMC1559924,Figure_1,oa_package/5c/1f/PMC1559924.tar.gz,"['More importantly, for in vivo applications, intracellular entry\ninto the target cell within the diseased tissue is required and\nshould lead to appearance in the cytoplasm to silence the mRNA of\ninterest ().', 'Improvement through novel chemical modificationsEuropean Journal of Biochemistry20032708162816441269417629ChiuYLRanaTMsiRNA function in RNAi: a chemical modification analysisRNA200399103410481292325330HallAHWanJShaughnessyEERamsay ShawBAlexanderKARNA interference using boranophosphate siRNAs: structure-activity relationshipsNucleic Acids Research20043220599160001554563731BraaschDAJensenSLiuYRNA interference in mammalian cells by chemically-modified RNABiochemistry20034226796779751283434932CzaudernaFFechtnerMDamesSStructural variations and stabilising modifications of synthetic siRNAs in mammalian cellsNucleic Acids Research20033111270527161277119633HoshikaSMinakawaNKamiyaHHarashimaHMatsudaARNA interference induced by siRNAs modified with 4 -thioribonucleosides in cultured mammalian cellsFEBS Letters200557914311531181591908434HarborthJElbashirSMVandenburghKSequence, chemical, and structural variation of small interfering RNAs and short hairpin \nRNAs and the effect on mammalian gene silencingAntisense Nucleic Acid Drug Development2003132831051280403635PrakashTPAllersonCRDandePPositional effect of chemical modifications on short interference RNA activity in mammalian cellsJournal of Medicinal Chemistry20054813424742531597457836LayzerJMMcCaffreyAPTannerAKHuangZKayMASullengerBAIn vivo activity of nuclease-resistant siRNAsRNA20041057667711510043137MorrisseyDVBlanchardKShawLActivity of stabilized short interfering RNA in a mouse model of hepatitis B virus replicationHepatology2005416134913561588058838LiaoHWangJHBiomembrane-permeable and ribonuclease-resistant siRNA with enhanced activityOligonucleotides20051531962051620190739MuratovskaAEcclesMRConjugate for efficient delivery of short interfering RNA (siRNA) into mammalian cellsFEBS Letters20045581 363681475951740SoutschekJAkincABramlageBTherapeutic silencing of an endogenous gene by systemic administration of modified siRNAsNature200443270141731781553835941JudgeADBolaGLeeACHMacLachlanIDesign of noninflammatory synthetic siRNA mediating potent gene silencing in vivoMolecular Therapy20061334945051634399442CarsteaEDHoughSWiederholtKWelchPJState-of-the-art modified RNAi compounds for therapeuticsIDrugs2005886426471604437243de JongeJHoltropMWilschutJHuckriedeAReconstituted influenza virus envelopes as an efficient carrier system for cellular delivery of small-interfering RNAsGene Therapy20061354004111626756744Kimchi-SarfatyCBrittainSGarfieldSCaplenNJTangQGottesmanMMEfficient delivery of RNA interference effectors via in vitro-packaged SV40 pseudovirionsHuman Gene Therapy2005169111011151614990945UprichardSLBoydBAlthageAChisariFVClearance of hepatitis B virus from the liver of transgenic mice by short hairpin RNAsProceedings of the National Academy of Sciences of the United States of America200510237737781564034646DittgenTNimmerjahnAKomaiSLentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivoProceedings of the National Academy of Sciences of the United States of America20041015218206182111560806447GodfreyAAndersonJPapanastasiouATakeuchiYBoshoffCInhibiting primary effusion lymphoma by lentiviral vectors encoding short hairpin RNABlood20051056251025181557258648RalphGSRadcliffePADayDMSilencing mutant SOD1 using RNAi protects against neurodegeneration and extends survival in an ALS modelNature Medicine200511442943349RaoulCAbbas-TerkiTBensadounJ-CLentiviral-mediated silencing of SOD1 through RNA interference retards disease onset and progression in a mouse model of ALSNature Medicine200511442342850BotIGuoJVan EckMLentiviral shRNA silencing of murine bone marrow cell CCR2 leads to persistent knockdown of CCR2 function in vivoBlood20051064114711531588632451YiYHahmSHLeeKHRetroviral gene therapy: safety issues and possible solutionsCurrent Gene Therapy20055125351563870952PalmerDJNgPHelper-dependent adenoviral vectors for gene therapyHuman Gene Therapy20051611161570348453WolkowiczRNolanGPGene therapy progress and prospects: novel gene therapy approaches for AIDSGene Therapy20051264674761570376454JiaWZhouQViral vectors for cancer gene therapy: viral dissemination and tumor targetingCurrent Gene Therapy2005511331421563871755WurdingerTVerheijeMHBroenKSoluble receptor-mediated targeting of mouse hepatitis coronavirus to the human epidermal growth factor receptorJournal of Virology2005792415314153221630660256XiongZChengZZhangXImaging chemically modified adenovirus for targeting tumors expressing integrin {alpha}v{beta}3 in living mice with mutant herpes simplex virus type 1 thymidine Kinase PET reporter geneJournal of Nuclear Medicine20064711301391639119757MonahanPEJoossKSandsMSSafety of adeno-associated virus gene therapy vectors: a current evaluationExpert Opinion on Drug Safety20021179911290416358KappesJCWuXSafety considerations in vector developmentSomatic Cell and Molecular Genetics2001261 61471581246546659GuoSTschammerNMohammedSGuoPSpecific delivery of therapeutic RNAs to cancer cells via the dimerization mechanism of phi29 motor pRNAHuman Gene Therapy2005169109711091614990860S rensenDRLeirdalMSioudMGene silencing by systemic delivery of synthetic siRNAs in adult miceJournal of Molecular Biology200332747617661265426161LuoMCZhangDQMaSWAn efficient intrathecal delivery of small interfering RNA to the spinal cord and peripheral neuronsMolecular Pain20051291619120362PalAAhmadAKhanSSystemic delivery of RafsiRNA using cationic cardiolipin liposomes silences Raf-1 expression and inhibits tumor growth in xenograft model of human prostate cancerInternational Journal of Oncology2005264108710911575400663OmidiYBararJAkhtarSToxicogenomics of cationic lipid-based vectors for gene therapy: impact of microarray technologyCurrent Drug Delivery2005244294411630544664Urban-KleinBWerthSAbuharbeidSCzubaykoFAignerARNAi-mediated gene-targeting through systemic application of polyethylenimine (PEI)-complexed siRNA in vivoGene Therapy20051254614661561660365TakeiYKadomatsuKYuzawaYMatsuoSMuramatsuTA small interfering RNA targeting vascular endothelial growth factor as cancer therapeuticsCancer Research20046410336533701515008566TakeshitaFMinakuchiYNagaharaSEfficient delivery of small interfering RNA to bone-metastatic tumors by using atelocollagen in vivoProceedings of the National Academy of Sciences of the United States of America20051023412177121821609147367SongEZhuPLeeS-KAntibody mediated in vivo delivery of small interfering RNAs via cell-surface receptorsNature Biotechnology200523670971768PirolloKFZonGRaitATumor-targeting nanoimmunoliposome complex for short interfering RNA deliveryHuman Gene Therapy20061711171241640913069SchiffelersRMAnsariAXuJCancer siRNA therapy by tumor selective delivery with ligand-targeted sterically stabilized nanoparticleNucleic Acids Research20043219e1491552045870KimBTangQBiswasPSInhibition of ocular angiogenesis by siRNA targeting vascular endothelial growth factor pathway genes: therapeutic strategy for herpetic stromal keratitisAmerican Journal of Pathology20041656217721851557945971ElbashirSMHarborthJLendeckelWYalcinAWeberKTuschlTDuplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cellsNature2001411683649449811373684s and TablesThree levels of\ntargeting: preferably, siRNA should be targeted to the diseased\ntissue (I).']","Figure 1 Three levels oftargeting: preferably, siRNA should be targeted to the diseasedtissue (I). Within this tissue it should be delivered to thecorrect cell type for silencing the mRNA of interest (II).Following entry of the target cell, siRNA should be delivered tothe cytoplasm (and/or nucleus) to interact with the components ofthe RNAi machinery (III).",yes
PMC5575325,Figure_5,oa_package/82/0a/PMC5575325.tar.gz,"[' 5a, lanes 1, 2, 8, 9).', ' 5a, lanes 3, 4, 10, 11).', ' 5a, lanes 5, 6, 12, 13).', ' 5a, lanes 3 4 vs.', ' 5b).', ' 5a, lanes 10 11 vs.', ' 5b).', ' 5c, lanes 1 8).', ' -cleavage of PrPC modulates intracellular iron and is unique to polarized RPE cells.', '\nScrapie infection induces functional iron deficiency in the retinaSince PrPC mediates uptake of iron to the neuroretina, we next evaluated whether its conversion to PrPSc alters retinal iron homeostasis during scrapie infection.']","Figure 5 -cleavage of PrP modulates intracellular iron and is unique to polarized RPE cells. ( ) Deglycosylated lysates from vector and PrP -expressing M17 and RPE cells and human brain were probed with PrP-specific antibodies 3F4 and anti-C to estimate the relative abundance of full-length (FL), -cleaved (C1), and -cleaved (C2) forms in each sample. Probing with 3F4 that reacts with FL and C2 (Fig. ) reveals undetectable levels of C2 in M17 cells even after over-expressing PrP (lanes 1 & 2). RPE cells, on the other hand, show significantly more C2 in non-polarized cells (lanes 3 & 4), and almost complete cleavage of FL to the C2 form following polarization (lanes 5 & 6). The brain sample shows minimal levels of C2 as in M17 cells (lane 7). Probing with anti-C antibody that reacts with FL, C1, and C2 (Fig. ) shows FL and C1 in M17 and brain lysates (lanes 8, 9, 14), and mainly C2 in RPE samples (lanes 1013). Re-probing for TfR shows down-regulation following polarization of RPE cells (lanes 3 & 4 vs. 5 & 6). Re-probing for DMT-1, on the other hand, shows up-regulation in PrP -expressing non-polarized RPE cells (lanes 10 & 11), and down-regulation following polarization (lanes 10 & 11 vs. 12 & 13). Densitometric analysis of protein bands after normalization with -actin shows downregulation of TfR following polarization of RPE cells to less than half, and upregulation of DMT-1 in PrP -expressing non-polarized cells by ~3-fold. DMT-1 expression is minimal in polarized RPE cells. Values are meanSEM of the indicated n. ns, not significant, * <0.05, ** <0.01, p<0.01, p<0.001. Exposure of non-polarized RPE cells to FAC increases the ratio of C2 vs. FL that is more evident following deglycosylation (lanes 5 & 7 vs. 6 & 8; d). Re-probing for ferritin shows a significant increase following FAC treatment as expected (lanes 2, 4, 6, 8).",yes
PMC4085555,Figure_1,oa_package/43/72/PMC4085555.tar.gz,"['The microsomal TG transfer protein, MTP, facilitates apoB secretion[43]-[46] in part by addition of lipid to the polypeptide as apoB translocates into the lumen of the ER (A).', 'ER-associated degradation of ApoB.', 'Three lesions likely occur simultaneously, or in extremely close succession: (1) exposure of poorly lipidated apoB regions in the ER lumen, (2) prolonged residence in the translocon, and (3) exposure of apoB motifs to the cytosol (depicted in B).', 'The homo-hexamer AAA-ATPase p97 has been shown to facilitate removal of ubiquitinated apoB from the ER for proteasomal degradation (see C)[124],[154].', 'Remarkably, Ubxd8 recruits p97 for the extraction of apoB from lipid droplets for proteasomal degradation (D)[155].']","Fig. 1 ER-associated degradation of ApoB. A: Cotranslational lipid recruitment. Following engagement between the ribosome and translocation channel, apoB crosses the ER bilayer and enters the ER lumen. There, the growing polypeptide begins to acquire lipid ligands, forming a nascent lipoprotein. Lipidation of apoB is an MTP-dependent process. MTP is present in the lumen as a heterodimer with PDI. The ER chaperone BiP is associated with apoB regardless of apoB lipidation status. Efficient translocation allows the growing apoB peptide to begin forming a secretion-competent lipoprotein. Additional protein folding machinery associates with nascent apoB, as discussed in the text. B: Translocation arrest and substrate selection. If the supply of lipid ligands is inadequate to sustain the assembly of this apoB-containing lipoprotein, translocation of apoB into the ER lumen becomes delayed, or arrested. When inefficient lipidation occurs, hydrophobic regions of lumenal apoB may be exposed to the aqueous environment in the lumen. This leads to further BiP binding (and other luminal chaperones), prolonged exposure of apoB to the inside of the translocation channel, and sudden exposure of apoB motifs to the cytosol (looping out). Cytosolically exposed apoB associates with cytosolic chaperones such as Hsp70 and Hsp90. With regards to substrate selection for ERAD, one, two or all three of these events may contribute to triggering downstream events in ERAD. C: Ubiquitination and retrotranslocation of apoB. p97 has been shown to facilitate the proteasomal degradation of apoB. Gp78 (and possibly other ubiquitin ligases, including Hrd1) mediates the ubiquitination of apoB. The presence of a threshold number of ubiquitin moieties on apoB likely provokes the p97 complex to act on the target substrate. There may exist sub-ERAD levels of apoB ubiquitination, either in the form of short ubiquitin chains, or low number of chains per polypeptide. p97-dependent retrotranslocation of ERAD substrates appears to require temporary deubiquitination of the target peptide. The substrate then becomes ubiquitinated once more in order to stimulate further proteasomal substrate trafficking and processing events. It is unknown through which channel/mechanism apoB is removed from the ER, nor is it known which (if any) cytosolic ubiquitin shuttling proteins are involved in delivering apoB to the proteasome. D: Retrotranslocation via ER-associated lipid droplet. A secondary ERAD pathway has been for shown for apoB. It depends on MTP activity and the formation of a primordial apoB-containing lipoprotein. ApoB crescents have been described as an intersection of proteasomal and autophagic degradation of apoB. Movement of apoB from the ER membrane to the ER-associated lipid droplets requires Derlin-1. The membrane-associated hairpin protein Ubxd8 was found to mediate the extraction of apoB from these lipid droplet-associated crescents by p97. It is a remarkable discovery that lipidated apoB, found in an abnormal, cytosolic lipid droplet-associated state, is extracted in linear form and can be processed by the proteasome.",yes
PMC8551705,Figure_2,oa_package/55/85/PMC8551705.tar.gz,[],"FIGURE 2 H&Estained lung sections showing histopathology and its assessment. images of H&E-stained lungs at 40 magnification showing regions of pneumonitis (blue arrow), bronchitis (red arrow), epithelial injury (green arrow), and inflammation (purple arrow) along with their histological score for pneumonitis, inflammation, lung injury, alveolar epithelial cells, bronchitis, and overall disease score for different groups UI, I, TT, and AO on day 4 postinfection. * < 0.05, ** < 0.01, *** < 0.001 (one-way ANOVA).",yes
PMC9393189,Figure_4,oa_package/97/47/PMC9393189.tar.gz,"['Therefore, computed tomography (CT) of the chest was requested, which revealed a thickened pericardium with pneumopericardium, as shown in .', 'Pneumoprecadium on chest CT (A) and moderate pleural effusion (B).']",Figure 4 Pneumoprecadium on chest CT ( ) and moderate pleural effusion ( ).,yes
PMC8258683,Figure_3,oa_package/f2/ca/PMC8258683.tar.gz,"['We implanted single bipolar electrodes into the SCN to monitor the MUA (A).', 'MUA in untreated (Cont) mice exhibited robust diurnal and circadian rhythms, with most of the activity occurring during the day, in antiphase with WR (B, S2E, S5E, and S5F).', 'When treated with METH, mice still displayed circadian locomotor activity and exhibited robust rhythms of MUA in the SCN (B, C, and S2F).', 'METH did not affect the onsets of MUA in the SCN nor WR rhythms (D; MUA onset in Cont mice: 23.', 'However, METH dramatically reduced the amplitude of the MUA rhythms recorded in the SCN (E; the amplitude in Cont mice: 1.', 'In addition, METH delayed the offsets of MUA rhythms in the SCN and WR rhythms (D; MUA offset in Cont mice: 12.', 'Interestingly, METH only lengthened the free-running periods of the WR rhythms (F; MUA in Cont mice: 23.', 'In vivo MUA rhythms in the SCN of METH-treated mice.', ')5DiscussionIn this study, we recorded robust diurnal and circadian rhythms in electrical activity in the striatum that peaked during the night and broadly mirrored the rhythms that we measured in WR activity (', 'Finally, we found that METH altered the MUA rhythms recorded in the SCN, although the SCN fluctuated itself under METH exposure ().', 'Specifically, chronic METH treatment reduced the amplitude (E) and delayed the offset (', 'Our electrophysiological data fits extremely well with this model as we demonstrate that the rhythmic electrical output of the SCN is reduced under METH (E).', 'We confirmed some impacts on rhythm parameters in the SCN in METH-treated mice in this study (D and E), which could be explained in the context of feedback from locomotor activity.']","Fig. 3 MUA rhythms in the SCN of METH-treated mice. (A) A typical histological section indicates the recording site using a neutral red stain. The black arrowhead indicates the recording site in the SCN. The scale bar indicates 200m. (B) Representative actograms showing diurnal and circadian rhythms of MUA in the SCN, WR and merged in METH-untreated (Cont) and METH-treated (METH) mice. The red and blue actograms indicate MUA and WR, respectively. The mice were maintained in the LD cycle and then transferred into the DD condition. The lighting conditions are indicated at the top of the figure; open bars indicate light phase and closed indicate dark. (C) Representative integrated mean activities of MUA and WR in Cont and METH-treated mice were plotted according to LD cycle and DD condition. MUA in the LD cycle for 3 days was normalized to 24h in 30min bins; each point indicates a level of MUA counts at the time relative to average MUA counts at 24h. WR in the LD cycle for 3 days was plotted in 60min bins. Red line indicates the standard mean activity of MUA and WR. As in the LD cycle, each MUA and WR in the DD condition for 6 days were also plotted to CT. Data were shown as meansSEM in 30min bins (MUA) or 60min bins (WR). (D) The activity onsets and offsets of MUA in the SCN and WR in Cont and METH-treated mice were calculated in the LD cycle, respectively. * <0.05, Student's unpaired -test. (E) The amplitude of MUA in the SCN in Cont and METH-treated mice were calculated in the LD cycle. * <0.05, Student's unpaired -test. (F) The free-running periods of MUA in the SCN and WR in Cont and METH-treated mice were calculated. Differing letters between groups indicate significant differences ( <0.05, Tukey's test). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)",yes
PMC3412803,Figure_4,oa_package/7b/12/PMC3412803.tar.gz,"['Using this approach, we failed to identify any severe defects in kidney morphology and nephrogenesis ().', 'g004Histological analysis of CXCR7-deficient kidneys.', 'Despite the remarkable plasticity of CXCR4 and CXCR7 mRNA expression in nephrogenic tissues, we observed no obvious defects in cap mesenchyme derived epithelial structures of nephrons in CXCR4 and CXCR7 deficient mice (, ).']",10.1371/journal.pone.0042814.g004,yes
PMC4747165,Figure_8,oa_package/23/0f/PMC4747165.tar.gz,"['Schematic representation of LPS-induced LITAF transcription role in HSCs during NAFLDActivation of TLR-4 through binding of LPS leads to receptor dimerization and the recruitment of adaptor proteins, such as MyD88.']","Figure 8 Schematic representation of LPS-induced transcription role in HSCs during NAFLD Activation of TLR-4 through binding of LPS leads to receptor dimerization and the recruitment of adaptor proteins, such as MyD88. This triggers the engagement of several other protein complexes resulting in the activation of TAK1. Once activated TAK1 may promote: 1) phosphorylation and activation of p38MAPK which supports the LITAF-dependent production of IL-1 and IL-6; 2) nuclear translocation and activation of p65NF-B which regulates the production of TNF-. SB203580 may hamper p38MAPK activation resulting in the inhibition of LITAF phosphorylation, nuclear translocation and transcriptional activity.",yes
PMC9315962,Figure_3,oa_package/9a/82/PMC9315962.tar.gz,"['[19] HRCT assessment can best be \nevaluated in all three planes axial, coronal and \nsagittal ().', '[9] An example of a high-resolution computed tomography scan of the lung with the target \nlobe identified.', 'The integrity of the fissures \ncan be assessed, and possible EBV target(s) \ncan be preliminarily identified.']","Fig. 3 An example of a high-resolution computed tomography scan of the lung with the target lobe identified. The (A) axial, (B) coronal and (C) sagittal views of an HRCT obtained from a patient who was deemed an appropriate candidate for ELVR with valves. In this case, the fissures (F) were 98% intact, with 74% destruction of the left upper lobe (ULL), which had a volume of 2 320 mL, making it the ideal target lobe.",yes
PMC10663703,Figure_4,oa_package/b9/8f/PMC10663703.tar.gz,"['9 (A).', 'Furthermore, the cell proportion by tumorous derivation of each subpopulation was examined, and similar results were obtained: the cell proportions of C6-HPN and C12-TPPP3 in PCa samples were significantly higher than those in normal prostates, and the differences were huge (B).', 'Particularly, C6-HPN had a slightly higher AUC value and larger differences between the cell proportions in PCa and normal prostates compared to C12-TPPP3 (A, B).', 'The diagnostic capability and sample distribution of each malignant subpopulation.', 'We further analyzed the cell proportions by patient derivation and found that C2-HPGD was mainly derived from one patient labeled D5 (', 'Similarly, C10-KLK12 and C12-TPPP3 mostly originated from patients D15 and D4, respectively (C).', 'However, C5-AHCY, C6-HPN and C9-FTX were distributed in almost all of the patients, suggesting that they are more universal than other subpopulations (C).', 'The universality of subpopulational distribution in PCa patients was evaluated by the homogeneity and positive ratio, showing that C5-AHCY and C6-HPN were homogeneously distributed in all patients (D).']","Fig. 3 Identification of malignant cells by clinical correlation analysis of each subpopulation in PCa progression. (A) The expression levels of marker genes from each subpopulation examined by the integrated dataset of TCGA and GTEx. (B) The correlations between the highly expressed genes in each subpopulation and the DEGs of normal and tumorous prostate samples from public datasets. (C) Feature plot and (D) violin plot showing the expression levels of PCa highly expressed genes in each subpopulation of normal and tumorous prostates, respectively. * <0.05, ** <0.01, *** <0.001.",yes
PMC4110042,Figure_3,oa_package/8d/ac/PMC4110042.tar.gz,"['smeg or H37Ra caused only a marginal effect on 2-NBDG uptake rates (. 3A).', 'Depending upon the infecting strain and time p-i, this increase ranged from 2- to 8-fold greater than the rate seen in uninfected cells (A).', 'This effect was seen in both THP-1 (B C) and primary human monocyte-derived macrophages (HuM ) (The capacity of the infecting strain to enhance glucose uptake by the host macrophage (A C) could also additionally superimpose on the constraints of glucose availability, and contribute towards determining survival versus death of the infected macrophage.']",10.1371/journal.ppat.1004265.g003,yes
PMC11328004,Figure_342,oa_package/e2/f1/PMC11328004.tar.gz,[],Chart 285 Structures of Complexes,yes
PMC9577858,Figure_3,oa_package/2e/93/PMC9577858.tar.gz,"['Three weeks after the procedure, visual acuity improved to 20/20 ().', 'A: Color photo fundus 30 seconds following Nd YAG hyalodotomy, the hemorrhage drains inferiorly into vitreous, B: Color photo fundus 3 hours following Nd YAG hyalodotomy shows the inferior vitreous hemorrhage with complete clearing of the macular region, C: Color photo fundus 3 weeks following Nd YAG hyalodotomy shows complete clearing of the hemorrhage.', ')This case report has been reported in line with the SCARE criteria [2].']","Fig. 3 A: Color photo fundus 30 seconds following Nd YAG hyalodotomy, the hemorrhage drains inferiorly into vitreous, B: Color photo fundus 3 hours following Nd YAG hyalodotomy shows the inferior vitreous hemorrhage with complete clearing of the macular region, C: Color photo fundus 3 weeks following Nd YAG hyalodotomy shows complete clearing of the hemorrhage.. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)",yes
PMC4425867,Figure_8,oa_package/a4/b9/PMC4425867.tar.gz,"['5mo increased levels of beclin, heat shock cognate protein 70 (HSC70) and lysosome-associated membrane protein 2a (Lamp2a) ( 8), indicating an upregulation of autophagy.', '5mo MB treatment enhanced proteasome function in comparison to WT and untreated Tau K mice ( 8).', 'Preventive MB administration increases protein degradation.', 'MB s role as electron carrier and thus redox-cycling compound is widely discussed in the literature [52].', 'Whereas an increase in beclin and Lamp2a levels was detected, levels of HSC70 and PSMD13 were comparable to untreated age-matched Tau K mice ( 8).']","Figure 8 Preventive MB administration increases protein degradation. Levels of autophagy- (beclin, HSC70), lysosome- (lamp2a), and proteasome-related (PSMD13) proteins in hippocampus lysates of age-matched untreated Tau mice compared to MB-treated Tau mice for 14.5mo, 6mo and 3mo. -actin serves as loading control. Quantification of . While both preventive MB treatment strategies (14.5mo and 6mo MB) increase levels of beclin, HSC70, Lamp2a and PSMD13, therapeutic MB treatment for 3mo leads to a partial increase of beclin and Lamp2a whereas levels of HSC70 and PSMD13 remain unaffected in comparison to untreated Tau mice. Protein levels were normalized to -actin. Bars indicate mean valuesSEM. Statistics: one-way analysis of variances with post-hoc Newman-Keuls multiple comparisons test. Asterisks indicate significant differences in comparison to untreated Tau mice; *: p<0.05; **: p<0.01; ***: p<0.001; n, number of samples.",yes
PMC6892966,Figure_1,oa_package/9f/0d/PMC6892966.tar.gz,"['Cytospin preparations were prepared and treated with Wright-Giemsa stain for morphological neutrophils observation (Supplemental ).', 'As shown in Table 2 and A, patients with active TB disease had a significantly (p 0.', '22N/L ratio, neutrophil, and lymphocyte absolute count of the different cohort groups and correlation between the N/L ratio and absolute neutrophil and lymphocyte count.', 'As shown in Table 2 and B, N/L ratio was consistent with the associated significantly higher absolute neutrophils count (Median: 5.', '001 (C).', 'Wright-Giemsa staining showed that the great majority of cells in this population ( 95%) displayed a neutrophil morphology (Supplemental ).', '02761/full#supplementary-materialSupplemental Giemsa staining of LDN and NDNs neutrophils.']","Figure 1 N/L ratio, neutrophil, and lymphocyte absolute count of the different cohort groups and correlation between the N/L ratio and absolute neutrophil and lymphocyte count. N/L ratio , neutrophil absolute count , and lymphocyte absolute count of patients with active TB disease, cured TB patients, and H.D. Each dot represents the value of an individual subject. Each horizontal bar represents the median of each group. Correlation between the N/L ratio and absolute neutrophil count, analyzed by Spearman rank correlation test. Data shown are the values of each individual subject. Correlation between the N/L ratio and absolute lymphocyte count analyzed by Spearman rank correlation test. Data shown are the values of each individual subject. Significance of differences between groups was compared using Kruskal-Wallis test, < 0.05, < 0.001, < 0.0001.",yes
PMC9442524,Figure_3,oa_package/43/59/PMC9442524.tar.gz,['The maximum CT value for cancer and granuloma.'],"Figure 3 The maximum CT value for cancer and granuloma. CT, computed tomography; HU, Hounsfield units.",yes
PMC7398663,Figure_11,oa_package/8a/1a/PMC7398663.tar.gz,[],"Figure 11. AGK2 treatment increases the efficacy of anti-TB drug INH. ( ) Experimental plan for infection in BALB/c mice. ( ) CFU enumeration to determine the bacterial burden in the lungs, spleen and liver of infected mice. ( ) Schematic representation of the adjunct therapy experiment. A group of mice were infected with low dose of . Following a rest of 15 days, mice were either left untreated or were treated with AGK2, INH or both. After 15 days of treatment, mice were euthanized for CFU enumeration in the lungs. ( ) CFU from the lung homogenates of respective animals. ( ) Schematic representation of the AGK2 treatment infection model. ( ) CFU obtained from the lung homogenates of mice infected with MDR and XDR strains of with and without AGK2 treatment. The experiment was performed once with five mice in each group. Data is represented as meanSD (n=5). *p<0.05, **p<0.005, ***p<0.0005.",yes
PMC7694353,Figure_1,oa_package/89/6d/PMC7694353.tar.gz,"[' 1a) and human AD brain sections (', '1a).', '1b) but no co-expression of GFP within microglia (', '1b) or neurons (', '1b), confirming the specificity of viral transduction as has been reported previously with this paradigm [27].', 'CLU co-localizes with amyloid plaques in mouse models and human AD and is specifically overexpressed in astrocytes.', '01We next evaluated CLU protein levels to determine the degree of overexpression we achieved in cortex and hippocampus of 8-month-old WT and APP/PS1 animals, measured by a CLU-specific enzyme-linked immunosorbent assay (ELISA).', '1c).', '1c).']","Fig. 1 CLU co-localizes with amyloid plaques in mouse models and human AD and is specifically overexpressed in astrocytes. Extensive CLU immunoreactivity (red) was observed in amyloid deposits (green) in cortex of APP/PS1 animals and brain tissue of an AD individual. Scale bar, 50m. AAV-mediated specific expression of GFP (green) in GFAP-positive astrocytes (red) but not IBA1-positive microglia (red) or NeuN-specific neurons (red) in APP/PS1 animals. Scale bar, 100m. CLU protein levels were assessed in cortex and hippocampus of WT and APP/PS1 mice by enzyme-linked immunosorbent assay (ELISA). Data represent meanS.E.M. Cortex: WT : =6 mice/group (1003.70), WT : =6 mice/group (12711.99), APP/PS1 : =10 mice/group (1182.98), APP/PS1 : =10 mice/group (1504.84). Hippocampus: WT : =6 mice/group (1003.09), WT : =6 mice/group (14013.37), APP/PS1 : =10 mice/group (1032.96), APP/PS1 : =10 mice/group (1356.97). Two-way ANOVA with Tukeys multiple comparisons tests were used, * <0.05, ** <0.01",yes
PMC9470830,Figure_3,oa_package/cb/73/PMC9470830.tar.gz,['Abdominal CT comparison.'],Figure 3 Abdominal CT comparison. ( ) postoperative CT at five months; ( ) preoperative CT (arrow refers to the tumor).,yes
PMC3551781,Figure_12,oa_package/2e/b4/PMC3551781.tar.gz,[],"Figure 12 ) The oocyte (o) development in OSC culture is accompanied by satellite (black arrow) and neuronal (white arrow) cells. Black arrowheads indicate organelles mowing from the satelite cell into the oocyte and white arrowhead indicates neuronal extension. Note ZP staining of fibro-epithelial cells (f) but no expression of ZP proteins at the oocyte surface (red arrowhead). Sperm associate (arrowheads) with fibroepithelial cells ( ) but not with the oocytes ( ). and ) The parthenote shows a trophectoderm (te), blastocoele (bc), and inner cell mass (icm). ( ) shows staining for ZP proteins, ( , and ) are live cultures in phase contrast (PhC), ( ) is a DAPI staining of the fixed culture. Adapted from[ ], Cambridge University Press, and unpublished observations.",yes
PMC3082766,Figure_1,oa_package/1c/e9/PMC3082766.tar.gz,['Decreased number of orexin-producing cells in the LH and narcoleptic phenotype of O/E3-null mice.'],"Figure 1 Orexin-A immunostaining of brain coronal sections show orexin-producing cell bodies in the lateral hypothalamic region of Wt animals [ , bregma 1.70mm, 3rdV denotes the lumen of the 3rd ventricle). In O/E3-null narcoleptic mice, orexin-A-positive cell bodies are drastically reduced in number and are restricted to a smaller region of the LH . Representative EEG/EMG recordings of transitions to REM sleep obtained during the dark period from Wt mice reveal that REM sleep is preceded by nREM sleep . Representative recordings from O/E3-null animals show direct transitions to REM sleep (DREM), where REM sleep is immediately preceded by an epoch of wake . Representative hypnograms derived from the EEG/EMG recordings show multiple DREM episodes occurring in O/E3-null mice , while none can be detected in Wt animals .",yes
PMC5683324,Figure_1,oa_package/e8/85/PMC5683324.tar.gz,"[' 1 summarizes the major pathological mechanisms contributing to motor neuron injury in ALS.', 'Molecular mechanisms in the pathology of amyotrophic lateral sclerosis.', 'b Schematic representation of ALS affected spinal cord motor neuron: 1) Astrocytes are not able to support neuronal functions and impaired glutamate clearance leads to neuronal excitotoxicity; 2) Defects in protein degradation pathways and disturbances in RNA processing result in protein aggregate formation, RNA toxicity and mitochondrial dysfunction; 3) The secretion of pro-inflammatory cytokines by predominant M1 activated microglia contributes to the development of an inflammatory milieu; 4) Failure of axonal architecture and transport functions, together with the alteration of the physiological role of oligodendrocytes results in 5) synaptic failure, denervation and finally, muscle atrophy\nThe complex heterogeneity of ALS, where several molecular mechanisms contribute to the pathology, enables various opportunities for therapeutic intervention.']","Fig. 1 Molecular mechanisms in the pathology of amyotrophic lateral sclerosis. Schematic representation of healthy spinal cord motor neuron. Schematic representation of ALS affected spinal cord motor neuron: 1) Astrocytes are not able to support neuronal functions and impaired glutamate clearance leads to neuronal excitotoxicity; 2) Defects in protein degradation pathways and disturbances in RNA processing result in protein aggregate formation, RNA toxicity and mitochondrial dysfunction; 3) The secretion of pro-inflammatory cytokines by predominant M1 activated microglia contributes to the development of an inflammatory milieu; 4) Failure of axonal architecture and transport functions, together with the alteration of the physiological role of oligodendrocytes results in 5) synaptic failure, denervation and finally, muscle atrophy",yes
PMC11598744,Figure_1,oa_package/cb/a3/PMC11598744.tar.gz,[],"FIGURE 1 FTS reduces the expression of proinflammatory factors and microglial activation in A mice. (AD) Levels of the inflammatory molecules IL1, IL6, TNF, and NO in the control mice and A mice treated with FTS. (EG) mRNA expression levels of the inflammatory factors IL1, IL6, and TNF in control mice and A mice treated with FTS. (H) Representative images of hippocampal microglia (Iba1; red, right) and nuclei (DAPI; blue, right), with representative images in the square in the middle (left). (I) The bar graph shows the Iba1positive area fractions (%, per field) in the control mice and A mice (right). (AI) * <0.05 and ** <0.01 vs. control mice, # <0.05 and ## <0.01 vs. A mice.",yes
PMC8271278,Figure_2,oa_package/5d/9d/PMC8271278.tar.gz,"['Visualization of the right lung baseVisualization of the left lung baseLung sliding is the respiration-related movement of the pleural line seen as a shimmering mobile hyperechoic line, which is the interface of the lung with the visceral pleura and the intercostal muscle.']",Figure 2 Visualization of the right lung base,yes
PMC3513847,Figure_1,oa_package/a5/d3/PMC3513847.tar.gz,"['Magnetic resonance imaging (MRI) scans revealed a 3 cm extra-axial, heterogeneously enhancing mass in the right parieto-occipital region with edema of the adjacent parenchyma [].', 'Preoperative (2008) axial (a), coronal (b), and sagittal (c) postcontrast T1-weighted magnetic resonance imaging scans show a 3 cm extra-axial, heterogeneously enhancing mass in the right parieto-occipital regionThe patient was treated with monotherapy of vincristine.']","Figure 1 Preoperative (2008) axial (a), coronal (b), and sagittal (c) postcontrast T1-weighted magnetic resonance imaging scans show a 3 cm extra-axial, heterogeneously enhancing mass in the right parieto-occipital region",yes
PMC6250849,Figure_3,oa_package/2d/fa/PMC6250849.tar.gz,[],"FIGURE 3 The example of brain extract HPLC chromatogram with Glu, Gln, and GABA peaks marked with ovals is on the left hand-side of the figure and the example of brain extract NMR spectra with indicated signals from Glu, Gln, and GABA is on the right hand-side of the figure.",yes
PMC11363383,Figure_3,oa_package/31/b5/PMC11363383.tar.gz,"['3.', 'Diagram of the process of cropping WSI into tiles.', 'figure (c) shows the tiles obtained by cropping from the annotated section, the number of patches obtained by cropping is proportional to the area of the annotated areaDifferent categories of tiles obtained by cropping, A for PTC, B for FTT, C for MTC, D for ATC, and E for NTC, with a pixel size of 224 224Deep neural networksAccording to the research content and its applicability, what we need is a neural network framework with high accuracy while ensuring low cost.']","Fig. 3 Diagram of the process of cropping WSI into tiles. shows the WSI of the original frozen thyroid nodule, and shows the annotation of the contours by the pathologist, the green contour is the ROI area, i.e., the area with lesions and non-cancerous areas can also be present in the slides with lesions. figure ( ) shows the tiles obtained by cropping from the annotated section, the number of patches obtained by cropping is proportional to the area of the annotated area",yes
PMC6849138,Figure_7,oa_package/7b/c4/PMC6849138.tar.gz,"['()', '3.']","Fig. 7 and Sonographic assessment of the neck confirmed the presence of a multinodular goitre; there was marked and asymmetric enlargement and hyperplastic change on the left. A potential candidate for parathyroid gland enlargement was demonstrated on the right and a benign, dominant and partly-exophytic thyroid nodule described on the left. Subtraction image from nuclear scintigraphy demonstrates concordance on the right but also raised the possibility of an enlarged left-sided parathyroid lesion. The suspicion of an intra-thyroid parathyroid gland on the left was corroborated on venous sampling.",yes
PMC6759837,Figure_8,oa_package/1f/3a/PMC6759837.tar.gz,"['The images revealed, unexpectedly, that GE-ERES in mdx and WT are polarized, with ERES on the sarcolemmal side (), suggesting that cortical muscle GE are associated with an ER-like subsarcolemmal tubular system described by Jayasinghe et al.', 'Polarity of GE vs.']","Figure 8 Polarity of GE vs. ERES organization and correlation of their volume. Z-stacks of confocal images showing GE (GM130 staining, red) and ERES (Sec31 staining, green) were collected at increased resolution with Lightning and represented in 3D in the LASX software. The volume difference between WT and images is striking but in addition there is a polarization of the ERES-GE axis. In this representation the staining is opaque. The left panels show the staining as viewed from outside the fiber, the right panels, obtained by 180 deg flipping (see arrows), show the same image viewed from inside. It thus appears that the -Golgi cisternae, surrounded by Sec31, are on the cytoplasmic side. Bar: 10 m for the main panels and 5 m for the enlarged insets.",yes
PMC7645312,Figure_3,oa_package/71/25/PMC7645312.tar.gz,"['Histological examination of the untreated samples revealed extensive bone formation within the defects (a).', 'AD-SVF group to the defect site, on the other hand, resulted in less bone bridge repair (b).', 'aSuccessful Growth Plate Disruption and Bony Bar Formation.', ')abBony Bridge Formation on Intervention Group.', 'bTable 2Descriptive analyses of mean bone bridge diameter between three different groups.']","Fig. 3a Successful Growth Plate Disruption and Bony Bar Formation. Bony bar formation (yellow arrow) is seen at 28 days post-injury confirmed through hematoxylin-eosin (HE) staining. The bony bar is fully mature by day 28 post-injury. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)",yes
PMC9436517,Figure_1,oa_package/f7/21/PMC9436517.tar.gz,"['Histology was acquired from six\n18\nF-choline-avid lesions, showing one papillary thyroid carcinoma\n(\n\n)\n, one metastasis of renal cell carcinoma, and four benign etiologies (colloid cysts and hyperplastic nodules).', '\nMaximum-intensity projection of\n18\nF-choline positron emission tomography (PET) (\nA\n) and transaxial views of the thyroid gland on PET (\nB\n), computed tomography (CT) (\nC\n), and PET/CT fusion images (\nD\n) showing intense\n18\nF-choline uptake in the enlarged right thyroid lobe (\narrows,\nmaximum standardized uptake value 8.']","Fig. 1 Maximum-intensity projection of F-choline positron emission tomography (PET) ( ) and transaxial views of the thyroid gland on PET ( ), computed tomography (CT) ( ), and PET/CT fusion images ( ) showing intense F-choline uptake in the enlarged right thyroid lobe ( maximum standardized uptake value 8.2). Histopathologic examination revealed a pT3a papillary thyroid carcinoma.",yes
PMC11605496,Figure_3,oa_package/4a/a9/PMC11605496.tar.gz,[],"FIGURE 3 Illustration of an altered vessel direction in thick actinic keratosis. Clinical photographs, dermatoscopic images and enface DOCT scans of (A) actinic keratoses (AK) grade II and (B) III. Blue arrows mark the imaged lesions in the clinical photographs. The DOCT enface scan from (A) AK II illustrates a central vessel accentuation, while the DOCT scan from (B) AK III illustrates radiating vessels.",yes
PMC6116282,Figure_3,oa_package/58/79/PMC6116282.tar.gz,"['mexicana promastigotes from logarithmic into stationary phase () using a protocol detecting mainly short-chain polyP ( 300 residues).', 'From a stationary phase culture, parasites were diluted to a concentration of 5 x 105 parasites/ml, cultured over 7 days and cell density was measured every day (A, C).', 'In promastigotes of both Leishmania species tested, polyP was most abundant in late logarithmic growth phase promastigotes (day 3 post dilution), while it gradually decreased overtime in long term stationary phase cultures (day 4 to 7 post dilution) (B, D), suggesting that polyP synthesis occurred mainly in proliferating parasites, and consumed when stationary parasites were maintained in culture for a long period.', 'In contrast to polyP, VTC4 levels stayed constant during the entire promastigote stage, suggesting that VTC4 might be inactivated by post-translational regulation, or the degradation of polyP chains might be increased during stationary phase (E).', 'FIGURE 3: PolyP and VTC4 fluctuation during promastigote growth of L.']","Figure 3 FIGURE 3: PolyP and VTC4 fluctuation during promastigote growth of and and promastigote cell concentrations were counted in independent cultures. PolyP was extracted from cell lines, digested with polyphosphatase and P were quantified colorimetrically using malachite green. P concentrations represented polyP content of 3 x 10 cells for and 8 x 10 cells for Results of a pool of minimum 3 independent experiments were expressed as mean SD. Western blot analysis of 20 g of promastigotes pellet protein using the anti-cd- VTC4 antibody and anti--tubulin as loading control.",yes
PMC10658150,Figure_5,oa_package/34/0e/PMC10658150.tar.gz,"['1% 12 months postoperatively ().', 'Postoperative chest radiograph 12 months after surgery showing a cardiothoracic ratio of 43.']",Fig. 5 Postoperative chest radiograph 12 months after surgery showing a cardiothoracic ratio of 43.1%.,yes
PMC3518090,Figure_7,oa_package/8b/e2/PMC3518090.tar.gz,"['006""/>60-year-old female with swelling in upper arm.']",Figure 7 60-year-old female with swelling in upper arm. MRI was done on 1.5T Siemen's machine. Coronal T1WI showing large lobulated lesion which is hypointense in signal intensity.,yes
PMC6796515,Figure_5,oa_package/bd/cc/PMC6796515.tar.gz,"['Consistent with previous results, nGFP+ nuclei were also found positive for RNA foci (a), and accordingly, no changes in splicing pattern was observed in transplanted muscles (', 'Detection of toxic RNA foci in donor-derived myonuclei upon satellite stem cell transplantation.', ')4DiscussionAlthough several attempts have been made to improve the muscle pathology in DM1, to date, there is no effective treatment available.']","Fig. 5 Detection of toxic RNA foci in donor-derived myonuclei upon satellite stem cell transplantation. (a) Upper panels show representative images for the engraftment of satellite cells isolated from a H2B-eGFP reporter mouse, as shown by the presence of nGFP (green) positive for RNA foci (red). Lower panels show PBS-injected muscles, which are negative for nGFP and positive for the foci. DYS (white) allows myofiber visualization. Asterisks indicate donor-derived engrafted myonuclei. Scale bar is 20m. Mid Z projection is shown. (b-c) RT-PCR analysis shows alternative splicing pattern of exon 7A, exon 7 and exon 22 in NSG-HSA muscles injected with satellite cells or PBS (b), and respective quantification (c). Data are shown as mean+SEM. =3 transplanted muscles, one per mouse. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)",yes
PMC7664031,Figure_7,oa_package/5b/5c/PMC7664031.tar.gz,"[' 7a).', ' 7b).', 'Common altered signature as effect of multiple dose exposure to doxorubicine a Common altered up- and downregulated genes between P12 and P24 in Hs578T and MDA-MB-231.', 'b Pathway analysis of differentially expressed genes at P12 and P24 on Hs578T and MDA-MB-231 cell lines using String softwareAltered genes can regulate essential processes responsible for cancer development.', ' 7).']",Fig. 7 Common altered signature as effect of multiple dose exposure to doxorubicine Common altered up- and downregulated genes between P12 and P24 in Hs578T and MDA-MB-231. Pathway analysis of differentially expressed genes at P12 and P24 on Hs578T and MDA-MB-231 cell lines using String software,yes
PMC3405480,Figure_3,oa_package/2e/50/PMC3405480.tar.gz,"['3,6 -Dithiothalidomide (56 mg/kg i.']","Figure 3 . Representative flat images of neurons labeled for Arc mRNA ( ) within the DG, after behavioral exploration of a novel environment for 10min. Nuclei are counterstained in . The illustrates the numbers of Arc positive ( ) cells in animals infused with aCSF and treated with drug vehicle. The displays the numbers of Arc + ve cells in LPS-infused, vehicle-treated animals. The shows the numbers of Arc + ve cells in LPS-infused, drug-treated animals. The indicates 100m.",yes
PMC3858367,Figure_1,oa_package/f6/02/PMC3858367.tar.gz,"['g001Selective increase of plasmablasts (PBs) during relapse of NMO.', 'This result assured that the encircled population in A represented CD19intCD27highCD38highCD180- PB cells.']",10.1371/journal.pone.0083036.g001,yes
PMC3031591,Figure_2,oa_package/4b/d9/PMC3031591.tar.gz,"['g001""/>Correlations between Urinary Cell Number and Renal PathologyBXSB mice showed GLs and TILs, namely, the expansion of mesangial matrix, proliferation of mesangial cells, dilated tubules by urinary casts, and perivascular cell infiltration (a and b).', 'Glomerular damage score was used as an index of GLs and was comparable to urinary cell number, suggesting that the number of urinary cells significantly increased with glomerular damage score (c).', 'In addition, urinary cell number significantly correlated with urinary albumin; however, no correlation was detected with BUN and Cre (d f).', 'g002Comparison between renal condition and urinary cell number.', 'Although perivascular infiltration of inflammatory cells was observed in BXSB kidneys (a), inflammatory cell markers were not detected in BXSB urine.']",10.1371/journal.pone.0016472.g002,yes
PMC10962376,Figure_1,oa_package/8f/43/PMC10962376.tar.gz,"['It is important to note that the canonical and non-canonical pathways of AHR activation are not mutually exclusive, and they can often intersect and interact with each other to regulate gene expression and cellular responses in a context-dependent manner [10, 11] ().', 'The AHR signalling pathway.']","Figure 1 The AHR signalling pathway. The aryl hydrocarbon receptor (AHR) is a transcription factor that is activated by ligand binding. In its inactive state, AHR forms a complex with heat shock protein 90 (Hsp90), X-associated protein 2 (XAP2), c-Src, and p23 in the cytoplasm. Upon binding to the ligand, AHR undergoes conformational changes, leading to the formation of complexes with ARNT, which then translocate into the nucleus. The XRE is located in the genomic regulatory region of AHR target genes, and in addition to detecting the XRE in the nucleus, the AHR-ARNT complex also interacts with other transcriptional regulators to regulate the expression of several target genes. The AHR, or aryl hydrocarbon receptor, is a protein involved in the regulation of gene expression. It forms a complex with ARNT, or aryl hydrocarbon receptor nuclear transport protein, and binds to XRE, or exogenous response element, to activate the transcription of genes, including those encoding cytochrome P450 enzymes (CYP)",yes
PMC3590339,Figure_2,oa_package/3d/46/PMC3590339.tar.gz,"['55%) patients were accurately detected on 3D CT (the most up to 27 pieces in one patient; 1 mm to 15 mm and 1 mm to 10 mm in length and diameter), and the FBs were successfully removed by intervention (343 FBs) () or surgery (9 FBs) (', 'FB = foreign body, 3D = 3-dimensional, VAI = virtual anatomy imagingScrew cap (10 mm in diameter and 1 mm thickness) fell off and remained in soft tissues around knee for 8 years, after internal fixation of left knee, in 50-year-old female patient.']","Fig. 2 Screw cap (10 mm in diameter and 1 mm thickness) fell off and remained in soft tissues around knee for 8 years, after internal fixation of left knee, in 50-year-old female patient. Muscle view of VAI clearly shows relationship of FB with fibular head (dovetail arrow) and superficial veins around knee, and damage to biceps femoris tendon (black arrow). Diameter, thickness of FB, and its minimal interval to popliteal artery and fibular head are visualized accurately on arterial view . Patellar ligament (white arrow) is clearly visible. Differentiation of sesamoidea (curving arrow) and FB (dovetail arrow) is noted differently here. Imprint of FB (black arrow), adjacent subcutaneous veins, and surgical scar (dashed arrow) are displayed clearly on superficial view in accordance with actual conditions . Using Allis' forceps to clamp screw cap in its central hole, FB was removed successfully under guiding fluoroscopy without any complications. Muscle view of popliteal space 3D shows normal structures of biceps femoris tendon (black arrow), semitendinosus muscle (white arrow), gastrocnemius (left-right arrow), popliteal vein (dashed arrow), small saphenous vein (white dovetail arrow), common peroneal nerve (curving arrow), and tibial nerve (black dovetail arrow). FB = foreign body, 3D = 3-dimensional, VAI = virtual anatomy imaging",yes
PMC3389942,Figure_2,oa_package/54/cd/PMC3389942.tar.gz,"['Ultrasound examination (): right testis is enlarged volume of about 6 ml.', 'Leydig cell tumor is a rare cause of precocious puberty in boys (1 case in our population, ).', '.']","Figure 2. A 6-year-old boy with symptoms of gonadotropin-independent PP. Right testis enlarged, US a heterogeneous mass in the right testis, with significantly increased abnormal vascular flow in the central part. Left testis normal. Underwent surgery. Histologically: Leyding cell tumor.",yes
PMC10450366,Figure_3,oa_package/7f/dd/PMC10450366.tar.gz,['\nBreast color Doppler ultrasound images.'],"Figure 3 A and B: Images of the breasts at admission showing irregular masses on both sides, with incomplete margins, angulation, and burrs; C and D: Images of the breasts after six cycles of chemotherapy showing that the masses had significantly decreased in size, with uneven internal echo and no point-like strong echo.",yes
PMC9575967,Figure_3,oa_package/c7/05/PMC9575967.tar.gz,[],"Figure3. Examples of different tissue samples: (a) N, (b) PB, (c) UDH, (d) FEA, (e) ADH, (f) DCIS and (g) IC.",yes
PMC3613076,Figure_4,oa_package/85/5d/PMC3613076.tar.gz,"['And it had marginal effects in preventing white cell infiltration ().', '003""/>Impact of G31P, dexamethasone, or ceftazidime treatments on inflammatory pathology in guinea pigs with Klebsiella pneumoniae.']","Figure 4 Impact of G31P, dexamethasone, or ceftazidime treatments on inflammatory pathology in guinea pigs with . In the G31P-treated group, PMN decreased significantly in BALF compared with the hormones, or antibiotics-treated group (*indicates < 0.05). The data show that G31P, hormones, and antibiotics groups have a significant effect in the inhibition of MPO release from lung tissue (*indicates < 0.05). G31P decreased significantly the release of MPO in lung tissue compared with the antibiotics group (*indicates < 0.05). But there was no significant difference between the G31P and DXM groups.",yes
PMC9382797,Figure_1,oa_package/83/56/PMC9382797.tar.gz,"[' 1) and a hyper signal of the mammary and peri-aqueductal bodies (in FLAIR axial section) (', 'Coronal section of Cerebral MRI in FLAIR sequence showing: Hyper signal of the mammary bodies', ' 1).']",Fig. 1 Coronal section of Cerebral MRI in FLAIR sequence showing: Hyper signal of the mammary bodies,yes
PMC4015531,Figure_5,oa_package/ce/9c/PMC4015531.tar.gz,['Coronal images of an 84-year-old man.'],Figure 5 Conventional MRA. Steady-state 3D VIBE sequence showing a non-contrast-enhanced aneurysm (box).,yes
PMC9399646,Figure_2,oa_package/5f/ee/PMC9399646.tar.gz,[],FIGURE 2 Sex-Specific Pathological Features with Left Hippocampus as ROI. Highlighted areas display the statistically significant cortical regions between mild cognitive impairment (MCI) and cognitively normal (CN) ( < 0.001) normalized to a 110 scale. Orange arrows indicate the area of difference at the precuneus cortex. Panels display MMCI v MCN. Panels display FMCI v FCN.,yes
PMC10896693,Figure_6,oa_package/63/db/PMC10896693.tar.gz,"['Dorsal segment preserving left trisectionectomy (resection of hepatic segments 2, 3, 4, v5, and v8).']","Fig. 6 Dorsal segment preserving left trisectionectomy (resection of hepatic segments 2, 3, 4, v5, and v8).",yes
PMC4867492,Figure_3,oa_package/f9/88/PMC4867492.tar.gz,"['We found that the crypt morphology was largely unaltered following MybER-activation (Supplementary a).', 'Furthermore, IPC proliferation was similar to that prior to tamoxifen-treatment (Supplementary b).', 'Two weeks of Tamoxifen produced modest but not significant effects on proliferation (Supplementary a, d and e) when PCNA cells were scored according to crypt location or total number.', 'In view of such modest effects of activated MybER alone we postulated that homeostatic mechanisms might be restricting the in vivo effect of elevated Myb activity particularly in view of the increased Ccne1 expression in the colons of all MybER transgenic lines (Supplementary c).', 'Activated Myb accelerates AOM-driven tumorigenesisIn view of this association between elevated Myb and CRC patient outcome patients we treated MybER mice with tamoxifen for an extended period of time (7 months) and at this point started to detect sick mice that once examined revealed elevated aberrant crypt foci (ACF) and a modest increase in colon tumors (s 3a and b).', 'Cohorts of these mice were exposed to tamoxifen but their disease-free survival was not different to Apcmin/ mice alone on tamoxifen (c).', 'MybER mice were treated with tamoxifen over a period of 8 weeks, six of which also employed weekly colon carcinogen azoxymethane (AOM) injections26 (d).', '27 As predicted, we found that this population was expanded (e).', 'Subsequent exposure of tamoxifen-treated mice to AOM markedly accelerated colon tumorigenesis in MybER mice on either WT or Apcmin/ backgrounds (f) and led to more frequent, as well as larger tumors, on an Apcmin/ background (g).', 'Mucin production was significantly reduced in MybER tumors compared with AOM-induced tumors from WT mice, whereas only a trend toward increased proliferation was evident in the presence of MybER activation (s 3h j).', 'Hunching was exacerbated by MybER-activation in ApcMin/ :MybER mice probably owing to the fact that there is a significant increase in tumor size in the colon in these mice (g).', 'Supplementary MybER-activation accelerates CRC initiation and progression of AOM-driven CRC.']","Figure 3 MybER-activation accelerates CRC initiation and progression of AOM-driven CRC. ( ) ACF in tamoxifen-treated mice compared with WT mice on Tamoxifen at 7 months ( =3). ( ) Tumor number is slightly increased in mice ( =8). ( ) Activation of MybER in mice on an background does not influence survival. ( ) Diagram depicting the pre-treatment and continual access to tamoxifen in chow (8 weeks) and 2 weeks later the initiation of weekly AOM injections used to test the effect of Myb-induction in the initiation phase of CRC. ( ) Pre-treatment by tamoxifen increases the percentage of cells with high aldehyde dehydrogenase activity identified by FACS ( >5). ( ) Survival experiments were conducted to determine whether MybER-activation on a WT or ( >10) background translates into poorer survival outcome owing to AOM-induced CRC. Individual mice were harvested when they reached ethical end points defined by either bleeding from the anus, anaemia (pale feet), hunching, severe diarrhea, prolapsed anus or body weight loss >20%. Survival analysis revealed that MybER-activation on a WT and backgrounds significantly accelerated the initiation and progression of disease and reduced the life expectancy of mice after treatment. ( ) Tumors generated on an background in presence of MybER were significantly larger compared with tumors arising in mice and MybER-activation did not alter the number of tumors. ( ) Tumors derived from all groups were sectioned and analyzed by IHC for PCNA to assess cell proliferation and for goblet cell differentiation ( ) using periodic acid staining (PAS). Data presented for individual samples with means s.e.m.; * <0.05, ** <0.01. Survival curves were analyzed using log-rank (MantelCox) Test.",yes
PMC11466717,Figure_4,oa_package/79/96/PMC11466717.tar.gz,"['The gallbladder was carefully separated from the adherent duodenum, and an approximately 1-cm fistula was noted ().', 'Cholecystoduodenal fistula.']","Figure 4 Cholecystoduodenal fistula. A) This is the cholecystoduodenal fistula prior to division. B) After division, there was a 1-cm duodenotomy.",yes
PMC3131265,Figure_2,oa_package/7b/c3/PMC3131265.tar.gz,['g002Non-invasive bioluminescence imaging of Sendai virus infection in the respiratory tracts of living mice.'],10.1371/journal.ppat.1002134.g002,yes
PMC11629136,Figure_2,oa_package/9a/b0/PMC11629136.tar.gz,[],"FIGURE 2 Most frequent diagnoses (primary/secondary) at initial consultation as a proportion of participant number (1) Atopic dermatitis (AD): (a) generalised AD in an infant, (b) generalised AD in a toddler, (c) papular AD affecting the torso of a toddler and (d) lichenified flexural AD in a teenager. (2) Dermatophyte infections: (a) tinea corporis of the elbow, (b) tinea corporis of the neck and tinea capitis, (c) tinea capitis and (d) tinea pedis and onychomycosis. (3) Acne: (a/b) comedonal and papulopustular facial acne with postinflammatory hyperpigmentation and (c/d) comedonal and papulopustular torso acne with postinflammatory hyperpigmentation. (4) Bacterial skin infections (BSI): (a) primary impetigo, (b) secondary BSI of AD, (c) secondary BSI of tinea capitis and pediculosis capitis and (d) secondary BSI of scabies. (5) Postinflammatory dyspigmentation: (a) hyperpigmentation secondary to tinea corporis, (b) hyperpigmentation secondary to AD, (c) hyperpigmentation secondary to acne and (d) hypopigmentation secondary to AD.",yes
PMC7671298,Figure_1,oa_package/7b/40/PMC7671298.tar.gz,['Type 1 endometrial carcinoma: endometrioid carcinoma of the endometrium.'],"Figure 1 Type 1 endometrial carcinoma: endometrioid carcinoma of the endometrium. (A) H&E-stained sections at 100X magnification showing fused glandular configuration of tumor with focal solid area of growth (arrow). (B) 200X magnification showing gland formation (arrow). (C) 400X magnification showing low nuclear grade and absence of prominent nucleoli. H&E, hematoxylin and eosin",yes
PMC6916010,Figure_2,oa_package/e5/71/PMC6916010.tar.gz,['2.'],Fig. 2 Coronal DIR reconstructions presenting with bilateral DIR hypersignal in prechiasmatic ( ) and chiasmatic ( ) optic nerve segments (white arrows) compared to normal DIR hypointense prechiasmatic ( ) and chiasmatic ( ) optic nerve segments (black arrows with white rims),yes
PMC9375954,Figure_2,oa_package/68/8a/PMC9375954.tar.gz,['CECT: contrast-enhanced computed tomographyNuclear bone scan.'],"Figure 2 Nuclear bone scan. The images shownew areas of mild uptake in the left sacral alae (yellow open arrows) and small focal areas of uptake of the mid and lower thoracic vertebral bodies (orange open arrows), findings concerning metastatic bone lesions.",yes
PMC7516190,Figure_5,oa_package/21/37/PMC7516190.tar.gz,"['5"">Muscle Pathology after Induction of Dystrophin IsoformsThe exclusion of Dmd exon 23 from mdx mice reversed the dystrophic pathology in diaphragm, including central nucleation, muscle fiber necrosis, mononuclear cell infiltrate, and fibrosis, as shown by hematoxylin and eosin staining in sham-treated mdx mice ( 5).', 'However, severe dystrophic pathology was evident in mouse diaphragm cryosections after exclusion of dystrophin exons 58+59 or exon 70 in both C57 and mdx mice ( 5).', 'A much lower percentage of central nucleation and degree of muscle fiber necrosis was seen in the diaphragm cryosections from mice expressing the dystrophin variant encoded by the transcript missing exons 56+57, compared to sham-treated mice ( 5), suggesting a milder dystrophic pathology.', ' 5Evaluation of Muscle Pathology by Hematoxylin and Eosin StainingDiaphragm sections were stained by hematoxylin and eosin to reveal pathogenic changes in muscle architecture (scale bar, 100 m).']","Figure4 Immunofluorescent Analysis of the Expression of -Dystroglycan Immunofluorescent analysis of the expression of sarcolemmal -dystroglycan on diaphragm cryosections (scale bar, 50m). -Dystroglycan was visualized by anti--dystroglycan (Santa Cruz) labeled with Zenon Alexa Fluor 488 dye (Thermo Fisher Scientific). Images were captured under the Nikon microscope with the NIS-Elements software (Nikon Instruments). C57, C57BL/10ScSn; , C57BL/10ScSn .",yes
PMC5995391,Figure_7,oa_package/12/fc/PMC5995391.tar.gz,"['A potential scheme for the kidney-heart interplay which may mediate changes in the mitochondrial structure and function in CKD with or without treatment with ARB is given .', 'g007A potential kidney-heart interplay which may mediate changes in mitochondrial structure and function in CKD.']",10.1371/journal.pone.0198196.g007,yes
PMC11377394,Figure_4,oa_package/b2/0c/PMC11377394.tar.gz,"[' 4A).', ' 4B).', 'Hematoxylin and eosin, 400DiscussionSurgical interventions for hypervascular tumors pose significant bleeding risks as a result of the tumors high vascularity.']","Fig.4 Histopathological findings. Macroscopically, the microcystic tumor was located on the pancreas head with no evidence of bleeding or necrosis. The tumor measured 684341mm. Microscopically, the cyst wall was lined by a single layer of cuboidal epithelium without atypia. Red blood cells (arrows) were among the epithelial cells. Hematoxylin and eosin, 400",yes
PMC2832107,Figure_4,oa_package/88/9f/PMC2832107.tar.gz,"['003""/>The subcellular features of the GRK2 immunoreactivity in the hippocampus of the rat subjected to 2-vessel occlusion.']","Figure 4 The subcellular features of the GRK2 immunoreactivity in the hippocampus of the rat subjected to 2-vessel occlusion. (a) Intact neurons show absence of any GRK2 immunopositive gold particles in their cytoplasmic matrix, X 15,000. (b) Neurons with the effect of chronic cellular hypoperfusion demonstrate the presence of a GRK2 overexpression (arrows) throughout the cell body, however, the intact mitochondria (M) were free from any GRK2 immunopositive gold particles, X 30,000. (c) -affected neuronal cell body show the presence of islands of GRK2 positive immunodecoration in the external membrane and in the matrix of damaged mitochondria and mitochondria-derived lysosomal structures (arrows), X 40,000. (d) Neurons with severe damage shows the presence of islands of GRK2 containing immunopositive gold particles that associated with the completely damaged (mitochondria-derived lysosomal structures) (arrows), but not with nondamaged mitochondria (intact and giant), X 40,000. (reprinted with permission of Neurotoxicity Research [ ]).",yes
PMC5656421,Figure_6,oa_package/4c/a5/PMC5656421.tar.gz,"['We therefore examined toluidine blue stained semithin section of CASK(+/ ) and control optic nerves during the first 3 weeks of postnatal mouse development (A).', 'A more clear and significant difference between the size optic nerves became apparent by the end of the first week of postnatal development and the difference from the wild type reaches the difference observed in adults by P22 (B).', 'CASK(+/ ) mice optic nerve display secondary reduction in size.']","Figure 6 CASK mice optic nerve display secondary reduction in size. (A) Representative images of toluidine bluestained semithin-sections derived from optic nerve of indicated ages and genotypes. Scale bars: 100 m. (B) Quantitation of optic nerve areas from mice of indicated age and genotype. Data are plotted as mean SEM, n = 3. (C) Quantitation of axonal density. Data are plotted as mean SD. (*P < 0.05; **P < 0.01). (D) Representative images of retinas from P6 mice stained with DAPI and RNA-RBPMS reveal no change in cell number in the ganglion cell layer of CASK mutants compared with controls. Scale bar: 25 m. (E) Quantification of DAPI- and RBPMS-positive cells in the ganglion cell layer in P6 mutant and control retina.",yes
PMC6102353,Figure_4,oa_package/6d/0b/PMC6102353.tar.gz,['HCA2 expression increases in blood and skin upon induction of experimental epidermolysis bullosa acquisita.'],"Figure 4 HCA expression increases in blood and skin upon induction of experimental epidermolysis bullosa acquisita. Quantification of monomeric red fluorescent protein (mRFP) cells in blood and ear skin of mice. The numbers of RFP cells are shown in percent of viable cells for blood and skin of naive mice or of animals on day 5 (d5) and 11 (d11) after first antibody injection. Mice were treated with vehicle of dimethyl fumarate (DMF) (50mg/kg, p.o., twice per day). * <0.05; ** <0.01 ( =5 mice, KruskalWallis Test with Dunns test). Representative dot plots from nave mice and animals at d5 (vehicle-treated group) are shown. Quantification of mRFP cells in immune cell populations of DMF- and vehicle-treated mice at d11. The numbers of mRFP cells are expressed as percent of CD45 CD11b Ly6G neutrophils, CD45 CD11b Ly6C monocytes, CD45 CD11b Ly6C monocytes, CD45 CD3 TCR T cells, CD45 CD3 TCR T cells, and CD45 CD3 NK1.1 NK cells. MeansSEM are depicted ( =5 mice).",yes
PMC4902832,Figure_8,oa_package/b6/ba/PMC4902832.tar.gz,"[' 8).', 'Scale bar = 200 mFor both mast cell tryptase and mast cell chymase, the highest cell density and fluorescence density were observed at day 0.']","Fig.8 Immunohistological analysis of mast cell chymase expression in an organ culture model using peroxidise staining. Day 0, Day 3, Day 7 and Day 10 untreated biopsies untreated biopsies (no formulation used) were included as a control. Mast cell numbers were reduced in cream and cream after 7 and 10days, compared with treated and untreated controls. =200m",yes
PMC8177680,Figure_8,oa_package/11/78/PMC8177680.tar.gz,['Cervical internal carotid artery aneurysm.'],Figure 8 Cervical internal carotid artery aneurysm. An 82-year-old male with swallowing disturbance. CT angiography (coronal/sagittal in ; axial in ) showing a vascular loop with a partially thrombosed aneurysm in the right internal carotid artery (arrows in and ). The lesion is along the course of the hypoglossal nerve.,yes
PMC6929255,Figure_3,oa_package/12/67/PMC6929255.tar.gz,['(A) US of the neck showed a 3 cm x 3 cm x 4 cm hypoechogenic change with a slightly heterogenic content.'],"Video 1 CEUS - kidney trauma. CEUS, contrast-enhanced ultrasound",yes
PMC8635097,Figure_3,oa_package/fc/73/PMC8635097.tar.gz,[],"Figure3 nPM exposure exacerbates white matter blood brain barrier leakage after bilateral carotid artery stenosis. Corpus callosum blood brain barrier (BBB) permeability was measured by using dynamic contrast enhanced MRI. Distribution of gadolinium-based contrast agent was followed during the scan and regional BBB transfer constant (K , x10 min ) was mapped and quantified in the corpus callosum area. In panel n=6 in filter, nPM and nPM+BCAS groups and n=5 in filter+BCAS group. BBB leakage was visualized postmortem by staining for extravascular IgG deposits. IgG positive deposits (red) outside lectin (white) positive blood vessels were quantified and presented as integrated density . In panel n=12 in all groups. Data is presented as violin plot with median and quartiles. 1-way ANOVA and Bonferroni tests were used for statistical testing. Scale bar = 50 m in panel . CC, corpus callosum; nPM, nanoparticulate matter; IgG, Immunoglobulin G; IntDen, integrated density.",yes
PMC3726683,Figure_3,oa_package/75/54/PMC3726683.tar.gz,"['We then evaluated ELAVL1/HuR protein levels finding that, besides ELAVL1/HuR mRNA, ELAVL1/HuR-siRNA also counteracted MG-132-induced ELAVL1/HuR protein accumulation (A).', 'Moreover, the MG-132-induced increase of SQSTM1/p62 protein was counteracted in ELAVL1/HuR silenced cells (B), for the first time suggesting that the positive regulation of SQSTM1/p62 expression, during proteasomal inhibition, requires the specific presence of ELAVL1/HuR protein.', 'g003The MG-132-mediated upregulation of SQSTM1/p62 protein expression requires the specific presence of ELAVL1/HuR protein.']",10.1371/journal.pone.0069563.g003,yes
PMC8668919,Figure_6,oa_package/6f/a1/PMC8668919.tar.gz,"['6a, b).', '6c).', '6d).', '6e).', '6f, g, PPD was significantly lower in the h -syn group than in the control group (', '6f, unpaired t test, t(30) = 3.', '6g, unpaired t test, t(31) = 0.', ' -Synuclein aggregates reduce paired-pulse depression of light-evoked IPSCs recorded from mitral cells and decrease granule cell activity in the OB.', 'To further confirm that -synuclein aggregation affects neural activity in GCs, we tested whether the excitability of GCs was altered by -synuclein overexpression.', '6h).', '6i, two-way ANOVA, F(1, 22) = 5.', '6j, unpaired t test, t(22) = 2.', '6k, unpaired t test, t(22) = 2.']","Fig. 6 -Synuclein aggregates reduce paired-pulse depression of light-evoked IPSCs recorded from mitral cells and decrease granule cell activity in the OB. , Low-magnification (scale bars=100m) ( ) and high-magnification (scale bars=20m) ( ) images showing granule cells labeled with mCherry in the OB. EPL external plexiform layer, MCL mitral cell layer, GCL granule cell layer. Schematic of the light-evoked IPSC electrophysiological recording. Representative IPSCs recorded from mitral cells when 2Hz (left) and 20Hz (right) light illumination was applied. Representative traces of paired-pulse IPSCs. Quantitative analysis of the paired-pulse depression of light-evoked IPSCs recorded from mitral cells from the control and h-syn groups (control: 19 cells from 8 mice; h-syn: 14 cells from 6 mice). Quantitative analysis of the amplitude of the first light-evoked IPSC. Representative current injection-evoked APs in granule cells. Quantitative analysis of the frequency ( ), onset latency ( ), and membrane potential ( ) elicited by positive current injections (13 cells from 5 mice for the control and h-syn groups, respectively). mIPSC miniature inhibitory postsynaptic current; * <0.05; ** <0.01; n.s. not significant. Data are presented as meanSEM.",yes
PMC10502336,Figure_4,oa_package/d5/31/PMC10502336.tar.gz,"['(A, B).', 'Hematoxylin-eosin staining (x200) showed spindle cells without atypia, corresponding to fibroblasts.', 'In contact with these bone lamellae, there are multinucleated giant cells of osteoclastic type (A).', 'Hematoxylin-eosin staining (x40) (B).', 'Once the diagnosis made, a second operation was scheduled under general anesthesia.']","Fig. 4 Hematoxylin-eosin staining (x200) showed spindle cells without atypia, corresponding to fibroblasts. In contact with these bone lamellae, there are multinucleated giant cells of osteoclastic type (Fig. 4A). Hematoxylin-eosin staining (x40) (Fig. 4B).",yes
PMC10401315,Figure_4,oa_package/6b/96/PMC10401315.tar.gz,"['PET-CT, axial image of the right hydronephrosis.']","Figure 4 PET-CT, axial image of the right hydronephrosis.",yes
PMC7509698,Figure_15,oa_package/a4/31/PMC7509698.tar.gz,[],Figure 15 28-year-old man with triceps tendon rupture. Sagittal oblique proton density-weighted fat-suppressed magnetic resonance imaging shows a high-grade tear of the conjoined tendon of the lateral and long heads of the triceps with proximal retraction (arrow). An associated osseous avulsion fragment is evident in the retracted tendon stump with oedema (arrowhead),yes
PMC8659713,Figure_2,oa_package/98/8b/PMC8659713.tar.gz,"['(A).', '(B and 2C).', '(D).', 'g002Reduced parasite accumulation in SBP-1 KO infected mice compared to WT infected mice.']",10.1371/journal.ppat.1010114.g002,yes
PMC4494914,Figure_5,oa_package/53/98/PMC4494914.tar.gz,['Restored renal Smad7 inhibits AA-induced renal fibrosis and inflammation in Smad7 KO mice at day 42 after induction of AANA and B: Renal collagen I and -SMA mRNA and protein expression by real-time PCR and western blot analysis.'],"Figure 5 Restored renal Smad7 inhibits AA-induced renal fibrosis and inflammation in Smad7 KO mice at day 42 after induction of AAN and : Renal collagen I and -SMA mRNA and protein expression by real-time PCR and western blot analysis. and : Renal infiltration of F4/80 macrophages and CD3 T cells detected by immunohistochemistry. and : MCP-1 and TNF mRNA expression detected by real-time PCR. Results show that compared to Smad7 KO mice with chronic AAN without treatment (AAN-UT) or treated with vector control (AAN-VC), restored renal Smad7 on Smad7 KO with AAN (AAN-Smad7) largely blocks renal fibrosis and inflammation. Data are expressed as mean SE for groups of 6 mice. * < 0.05, ** < 0.01, *** < 0.001 compared with the saline control mice. < 0.05, < 0.01, < 0.001 compared with Smad7 KO mice with chronic AAN treated with or without VC. Magnification: x400.",yes
PMC459230,Figure_5,oa_package/e7/79/PMC459230.tar.gz,['Dual labeling of Aquaporin 1 and N-cadherin in the inner cortex.'],Figure 5 Dual labeling of Aquaporin 1 and N-cadherin in the inner cortex. Samples were processed for the dual labeling of the proximal tubule marker aquaporin 1 and N-cadherin as described in the Methods and then viewed using a 40 or a 100 objective. Panels A-D show lower images of the same field. A: Aquaporin 1; B: N-cadherin; C: Overlay of images A and B; D: Phase contrast image of the same field. Panels E and F show the dual labeling in a different field viewed at higher magnification. E: Aquaporin 1; F: N-cadherin. Original magnification = 174 (Panels A-D) and 435 (Panels E and F).,yes
PMC10805392,Figure_2,oa_package/29/72/PMC10805392.tar.gz,[],"FIGURE 2 RIC significantly diminished brain injury. (A, B) Assessment of brain damage using TTC staining and neurological deficits evaluation. (A) RIC induced a significant decrease in infarct volume in the penumbra region of the ischemic territory supplied by MCAO at day 3 post reperfusion. The variables between the stroke and RIC groups were analyzed using an unpaired test. (B) RIC significantly reduced neurological deficits, as detected by both 5 and 12point systems. (C) Apoptotic cell death as detected by the TUNEL assay. In the sham group (top two panels), few apoptotic cells were observed (white arrowheads indicating TUNEL+ positive cells). In the stroke group, apoptotic cells were significantly increased (*** < 0.001, middle two panels), which were significantly reduced by RIC (*** < 0.001, bottom two panels). Scale bars represent 100 m for low and 20 m for high magnification images. (D, E) Cell death (D) and LDH levels (E) were quantified using ELISA. Data, presented as mean SEM. * < 0.05, indicate a significant decrease in both cell death and LDH upregulation as a result of RIC treatment.",yes
PMC8891638,Figure_2,oa_package/f3/6f/PMC8891638.tar.gz,[],FIGURE 2 Magnetic resonance angiography (MRA) of a 68-year-old woman shows moderate stenosis of bilateral middle cerebral arteries. Mean cerebral blood flow (mCBF) demonstrates decreased blood perfusion in bilateral temporal and parietal lobes. Prolongation of arterial transit time (ATT) is demonstrated at the same anatomical regions on the ATT map.,yes
PMC10685462,Figure_7,oa_package/17/1b/PMC10685462.tar.gz,"[' 7 shows the accuracy and loss graphs created during the training and testing, procedures.', '\nAccuracy and loss graphs for the second CNN model\nAfter 30 epochs, on the training set the model achieved results with 100% accuracy, 100% recall, approximately 100% precision, and an F1 score of 100%.']",Fig. 7 Accuracy and loss graphs for the second CNN model,yes
PMC4182477,Figure_2,oa_package/a0/77/PMC4182477.tar.gz,"['05, A and 3A).', '172, B and 3B), but it was higher than that of two non-diabetic groups (p 0.', '651, C and 3C), but were significantly decreased compared to two non-diabetic group (p 0.', 'g002The expression levels of target gene mRNA.', 'Our results revealed that the level of GIGYF2 expression was significantly up-regulated in hippocampus tissue of diabetic mice (A and 3A), which is correlated with manifestations of diabetes-associated cognitive impairment (', 'Meanwhile, we found that the level of total IGF1R expression (C and 3C) was significantly decreased, but the level of phosphorylated IGF1R and the level of phosphorylated ERK1/2 was significantly increased in DM + shRNA group.', 'However, our present studies showed that the level of total IGF1R (C and 3C) and phosphorylated IGF1R (', '3C) were both comparable to normal level in hippocampus, but the level of total IGF1R in hippocampus of these mice was still significantly decreased (C and 3C).', 'However, disruption of GIGYF2 expression in hippocampus had no obvious effects on Grb10 abundance (B and 3B), indicating that GIGYF2 expression might have no obvious relationship with Grb10 expression.']",10.1371/journal.pone.0108559.g002,yes
PMC6713087,Figure_4,oa_package/f1/cb/PMC6713087.tar.gz,['.'],"Figure 4. Arrhythmic sudden death due to arrhythmogenic cardiomyopathy (segmental form) in a 26-year-old athlete. (A) Anterior view of the right ventricular outflow tract which appears mildly dilated; (B) Cross-section of the heart showing the absence of right ventricular free wall aneurysms: note the spotty involvement of the posterior right ventricular free wall; (C) Histology of the right ventricular outflow tract: note the regional loss of myocardium with fibro-fatty replacement (Trichrome Heidenhain, 2.5); (D) Histology of the posterior right ventricular free wall: note the fibro-fatty replacement of the myocardium in the absence of wall thinning (Trichrome Heidenhain, 5).",yes
PMC7205432,Figure_3,oa_package/5e/20/PMC7205432.tar.gz,"['Treatment of AC16 cells with either 100 ng/mL LPS or 1 g/mL LPS for 6 hours increased BNP mRNA levels compared with vehicle control (A).', 'The effect of LPS on BNP expression was abrogated upon combined treatment of AC16 cells with LPS (1 g/mL) and the pharmacological JNK inhibitor SP600125 (JNKi, 1 g/mL) (B).', '2-fold) (C).', 'Infection of AC16 cardiomyocytes with adenovirus containing c-JunASP, a constitutively active c-Jun isoform (16) with JNK phosphorylation sites that have been substituted to the phospho-mimetic aspartic acid (Ad c-JunASP), increased BNP mRNA levels 5-fold (D).', 'On the other hand, treatment of AC16 cells with adenovirus expressing c-JunALA that has the same amino acids substituted to alanine, which cannot be phosphorylated (Ad c-JunALA), reduced BNP mRNA by 3-fold (E).', 'In silico analysis of the mouse Nppb and human NPPB promoters ( 2000 to +100 bp) for potential AP-1 sites (Genomatix software) identified the presence of 4 on the mouse promoter and 7 on the human promoter (F).', 'Alignment of the mouse and human promoters (PromoterWise software) identified a single conserved AP-1 site located 400 bp upstream of the transcription start site of the mouse and human promoter (F).', 'ChIP analysis in AC16 cells infected with Ad c-JunASP showed a 3-fold enrichment of the 400 AP-1 site compared with control cells infected with Ad-GFP, thereby confirming that c-Jun binds to the NPPB promoter via the 400 AP-1 site (G).', '6-fold in response to Ad c-JunASP but not Ad c-JunALA (H).', 'We next sought to determine if the same effect would occur following activation of endogenous c-Jun by infecting AC16 cells with Ad-JNK2 2 (I).', 'As with c-JunASP, mutation of the AP-1 site in the human NPPB promoter negated the ability of JNK2 2 to stimulate luciferase activity (I).', 'Although plasma BUN was elevated in septic mice 12 hours after CLP, none of the 3 experimental setups, BNP-KO mice (Supplemental A), and septic C57BL/6 mice treated with either 19B3 (Supplemental B) or JNKi (Supplemental C) had lower plasma BUN levels compared with their respective septic control mice.', 'Activation of JNK signaling increases BNP production.']","Figure 3 Activation of JNK signaling increases BNP production. ( ) BNP mRNA levels in AC16 cardiomyocytes stimulated with 100 ng/mL or 1 g/mL LPS for 8 hours. = 4, control (Ctrl) wells; = 4, LPS (100 ng/mL) wells; = 4, LPS(1 g/mL) wells. * < 0.05 versus Ctrl by 1-way ANOVA with Tukey multiple comparisons. ( ) BNP mRNA levels in AC16 cardiomyocytes stimulated with 1 g/mL LPS and SP600125 (100nM) for 8 hours. = 5, Ctrl wells; = 6, LPS wells; = 5, LPS + SP600125 wells. ** < 0.01 versus Ctrl, < 0.01 versus LPS by 1-way ANOVA with Tukey multiple comparisons. ( ) BNP mRNA levels in AC16 cells infected with Ad-JNK22 (MOI, 10; 48 hours). = 4, Ad-GFP wells; = 5, Ad-JNK22 wells. * < 0.05 versus Ad-GFP by test. ( and ) BNP mRNA levels in AC16 cardiomyocytes infected with Adc-Jun ( ) or Adc-Jun ( ). For , = 4, Ad-GFP wells; = 8, Adc-Jun wells. For , = 11, Ad-GFP wells; = 9, Adc-Jun wells. * < 0.05, *** < 0.001 versus Ad-GFP by test. ( ) Schematic representation of the mouse and human promoters showing a conserved predicted AP1 motif located 400 bp upstream of the transcription start site, highlighted in yellow. ( ) Enrichment of the 400 AP1 site of human promoter with c-Jun in chromatin samples from AC16 cells treated with control Ad-GFP or Adc-Jun (ChIP-qPCR analysis). = 8 wells/group. ** < 0.01 versus Ad-GFP by test. ( and ) Firefly luminescence normalized to Renilla luminescence in AC16 cells transfected with pGL3-hNPPB (WT) or 400AP1 mutant, and infected with Adc-Jun or Adc-Jun ( ) or with Adc-Jun or Ad-JNK22 ( ). = 8 wells/group. *** < 0.001, **** < 0.0001 versus Ad-GFP; < 0.001, < 0.0001 versus Adc-Jun by 1-way ANOVA with Tukey multiple comparisons.",yes
PMC5644414,Figure_1,oa_package/ae/a4/PMC5644414.tar.gz,"['Ultrasonography of the abdomen revealed hepatomegaly with fatty changes and ascites [b]; 2D Echo imaging showed bilateral pleural effusion.', 'Clinical and microscopic image of the patient with leprosy.', 'The patient developed erythema nodosum over the legs [a] four days after admission.']","Figure 1 Clinical and microscopic image of the patient with leprosy. (a) Erythema nodosum over legs, (b) ascites, (c) anterior chamber aspirate showing acid fast bacilli (Ziehl-Neelsen stain, 1000), (d) Paraffin block of skin incisional biopsy showing granulomatous nodule in the dermis (H and E, 400).",yes
PMC10385607,Figure_1,oa_package/ab/bf/PMC10385607.tar.gz,"['In this study, we first produced scFv IF8, confirmed its homogeneity by SEC and SDS-PAGE (A C), and determined its biophysical characteristics.', '1 C (D,E) and a Cm of 2.', '43 M GdnHCl (F,G).', '1 nM; H,I).', '6 nM (J,K).', 'To further improve the stability of IF8, we grafted all CDRs and selected residues in the vicinity of the CDRs onto a framework with known stability [36,37] to yield the optimized scFv OPT (A and S1).', 'In comparison to scFv IF8, scFv OPT had an increased Tm (increase of 6 C; D,E) and Cm (increase of 0.', '45 M GdnHCl; F,G).', 'Importantly, scFv OPT retained a mid-nanomolar affinity for hALCAM (2-fold improved Kd; H,I) and directly competed with CD6 at levels equal to those of scFv IF8 (J,K).', 'In the next step, we randomized the CDR2 loops of scFv V2D7 and isolated the V200G1 and V20C10NC clones (Table 4, A).', 'Each selected clone was successfully produced in CHO cells, purified by protein A affinity chromatography, and recovered in a monomeric state to give a yield of 10 mg/mL with 95% purity (B,C).', '5 5 nM; H,I) and performed significantly better in the CD6 competition ELISA (2- to 3-fold reduced IC50; J,K).', 'However, in the case of the V200G1 and V20C10NC clones, the improved affinity came at the expense of reduced thermal and chemical stability (D G).', 'Improvements in Stability and Affinity Are Similar between Antibody Clones in scFv and db FormatsTo investigate whether the improvements in stability and affinity observed in the scFv format () would also remain apparent in the bivalent format, we produced the different clones (IF8, OPT, V2D7, V200G1, V20C10NC) in db format (A).', 'For example, the Tm and Cm of OPT in db format were again significantly improved compared to the values measured for IF8 in db format, whereas of the affinity-matured clones, only V2D7, but not V200G1 or V20C10NC, retained superior thermal and chemical stability in the db format (E,G and E,G).', 'The fact that the sequence of the optimized scFv OPT achieved more favorable PPC and PSH scores across the CDR vicinity in comparison to the starting sequence of scFv IF8 ( S1B) likely explains the associated stability improvements of the OPT fragment (E,G).', '00135753716Framework and affinity improvements to IF8 yield superiorly stable, high-affinity anti-ALCAM scFv fragments.']","Figure 1 Framework and affinity improvements to IF8 yield superiorly stable, high-affinity anti-ALCAM scFv fragments. ( ) Schematic representation of stability and affinity improvement steps performed in the generation of optimized scFv variants. ( ) Side-by-side biophysical and functional comparison of the original (IF8), framework optimized (OPT), and affinity-matured (V2D7, V200G1, V20C10NC) purified scFv variants. Color coding is as shown in ( ). ( ) SDS-PAGE under nonreducing conditions. Expected molecular weight of the scFvs: 24 kDa. ( ) FPLC profile on a Superdex 75 Increase column. ( ) Representative plot of thermal unfolding curves as assessed by nanoDSF. ( ) Quantification of T (mean SD of n = 34 independent measurements per variant). ( ) Representative plot of protein unfolding in GdnHCl as assessed by nanoDSF. ( ) Quantification of C (mean SD of n = 24 independent measurements per variant). ( ) Representative plot of the binding of the scFv fragments to immobilized hALCAM as assessed by single-cycle SPR. Sequential injection of the antibodies at 30 nM, 60 nM, 120 nM, and 240 nM. ( ) Quantification of the affinity constant (K ) on-rate and off-rate (mean from n = 2 independent runs). ( ) Representative plot of the CD6-Fc competition ELISA. ScFv variants outcompete CD6-Fc for binding to hALCAM at increased concentrations, reducing the CD6-Fc absorbance readout. ( ) Quantification of the IC from CD-Fc competition ELISA (mean SD of n = 414 independent experiments per variant). Statistics: One-way ANOVA, HolmSidak multiple comparison test ( , , ).",yes
PMC3314836,Figure_4,oa_package/92/b9/PMC3314836.tar.gz,"['On further intraoral examination, it was found that the patient had three supernumerary teeth lingual and distal to the second premolar in the lower right quadrant (A).', 'The radiograph showed an impacted supernumerary tooth on the left lower mandibular area, which was placed apical to the lower left second premolar (B).', 'The shape of the crown showed aberration in shape (C).', 'Case 4.']",Fig. 4 Case 4. A. Intraoral photograph shows three supernumerary teeth lingual and distal to the right mandibular premolars. B. Panoramic radiograph reveals an impacted supplementary tooth apical to the left mandibular second premolar. C. Intraoral periapical radiograph shows the presence of a dilacerated premolar-like tooth between the second premolar and the first molar.,yes
PMC4084682,Figure_2,oa_package/7c/0a/PMC4084682.tar.gz,"['In control brains, positive DPP10789 staining was predominantly associated with neurons in the CA1 region of the hippocampus (s 2(a), 3(a), and 3(b)).', 'These positive structures were most abundant in the hippocampal region, where staining was most intense in the CA1 and subiculum areas (s 2(b) 2(e) and 2(h)).', 'At high magnifications, three types of DPP10789 positive neurofibrillary tangle-like structures were observed: (1) granular staining in the neuronal soma ((j)), resembling pretangle phosphotau aggregates; (2) condensed intraneuronal staining (s 2(k), 2(l), and 2(o)), resembling intraneuronal tau NFTs; (3) less condensed extracellular staining (s 2(m), 2(n), and 5(h)), resembling extraneuronal tau NFTs and ghost tangles.', 'In addition, DPP10789 immunoreactivity was also detected significantly in dystrophic neurites surrounding amyloid plaques (s 2(d), 2(e), arrows, and 2(p)) and in neuropil threads ((q)).', 'Our immunohistochemistry studies revealed that in control brains DPP10789 immunoreactivity is located in the cell body of neurons throughout the grey matter of cerebral cortex and axonal fibre tracts of white matter (s 2(a) and 3(a)), suggesting that DPP10789 protein is widely distributed in the neuronal cells which is consistent with the expression of DPP10 mRNA reported in rat brain [28].', '001""/>Immunostaining of DPP10789 in free-floating sections of control and AD brains.']","Figure 2 Immunostaining of DPP10 in free-floating sections of control and AD brains. DPP10 immunostaining in the CA1 region of a control brain (a), CA1 and subiculum region (b, c, and d), CA2 (e), inferior frontal cortex (f), cingulated cortex (g), inferior temporal cortex (h), and inferior temporal cortex section blocked with antigenic peptide (i) in AD brains. DPP10 staining was found in granular structures in the neuronal soma (j), NFT with extensions into dendrites of pyramidal neurons (k), globose NFT (l), extracellular ghost tangle (m), flame shaped NFT (n), slender tangle of the subiculum with long extension into apical dendrite (o), plaque-associated dystrophic neurites (p), and neuropil threads (q). Scale bar is 200 m (b), 50 m (a, ci), and 10 m (jq). Arrows in (d) and (e) indicate staining in plaque-associated dystrophic neurites in AD brain.",yes
PMC7615119,Figure_17,oa_package/60/36/PMC7615119.tar.gz,[],"Fig.7 Vascular abnormalities and liver pathology are prevented by VEGFA antibody administration in mutants by ERK-independent mechanisms. , Experimental layout for the inducible deletion of in Cdh5 ECs and VEGFA antibody administration. , Confocal micrographs showing reduced CD31 or EMCN vascular immunostaining after anti-VEGFA treatment. , Stereomicroscope liver pictures. , Vessel density is reduced in mutants after anti-VEGFA treatment. , UMAPs and barplot showing the identified clusters and the percentage of cells for each cluster in indicated samples. , Unsupervised hierarchical clustering showing gene expression changes. , Dot plot ofthe top upregulated genes for each indicated gene set. , Violin plots of scRNA-seq data showing that anti-VEGFA treatment prevents the strong upregulation of and its target , The total number of ERG ECs, proliferation (Ki67 ERG ) and Esm1 expression (Esm1 ERG ) return to control conditions after VEGFA antibody administration. , Dot plot showing expression of flow/shear stress genes. , Number of upregulated genes for each contrast and Venn diagrams showing that when compared with loss, anti-VEGFA treatment has less effect on the upregulated genetic program. DEGs, differentially expressed genes. , GSEA hallmark analysis confirms the more moderate effect of anti-VEGFA treatment on the genetic program when compared with loss. , Experimental layout for the inducible deletion of and SL327 administration. , UMAPs and barplot showing the identified clusters and the percentage of cells for each cluster in indicated samples. , The administration of an ERK/MEK signaling inhibitor (SL327) results in reduced ERK phosphorylation. , Violin plot showing that SL327 treatment partially inhibits the generation of tip cells ( ). , The administration of SL327 does not change the frequency of proliferating Ki67 ECs (Ki67 ERG ). , Abnormal vasculature (CD31 EMCN ) associated with liver pathology still occurs after SL327. Data are presented as mean values s.d. For statistics, see Source Data File 1. Scale bars, 100 m, except in and upper panel, 1 mm.",yes
PMC9655885,Figure_7,oa_package/18/b1/PMC9655885.tar.gz,"['An Efficacy of 3-EA on Animal Model of Acute Brain Ischemia in RatsThe acute ischemic attack led to a profound depression in the neurological status of experimental animals assessed 1, 3, and 7 days after middle cerebral artery (MCA) occlusion (A).', '05; A), while showing no impact on the earlier stage of laboratory stroke development.', 'We registered congruous changes in the rate of damaged neurons and severity of tissue alteration (B,D) in the studied groups: 18.', 'Neurological disorder and brain tissue damage in experimental groups 7 days after MCA occlusion; staining by the Nissle method, 200; n = 6 in each group, data presented as mean SD, f p 0.']","Figure 6 Expression of genes regulating apoptosis ( , ) and oxidative stress activity ( , ). Gene expression in control cells are marked by dashed line for basal level. Gene expression in OGD/R cells are marked by dashed line for OGD/R level. Comparison of experimental groups regarding control or OGD/R group without 3-EA: n/s-data not significant ( > 0.05), * < 0.05, ** < 0.01 and *** < 0.001 when comparing experimental groups (ANOVA and Tukeys post-hoc test). Comparison of experimental groups relative to each other is indicated in red or black. The number of RNA samples is 3. The number of animals used for cell culture preparation is 3.",yes
PMC3737232,Figure_1,oa_package/ce/32/PMC3737232.tar.gz,"['ResultsPharmacologic Blockade of 5LO Ameliorates Cognition in 3 Tg MiceIn the Y-maze, 3 Tg mice receiving zileuton and the group treated with placebo did not show any significant difference in the total number of arm entries, suggesting that there were no differences in their general motor activity (A).', 'However, the number of alternation for the mice receiving the drug was increased when compared with their controls, as shown by their higher percentage values (B).', 'However, 3 Tg mice receiving zileuton had a significantly higher freezing time in the cued recall paradigm than the ones receiving placebo (D), but no differences were observed in the contextual recall (C).', 'However, in the probe trial 3 Tg mice treated with zileuton had significant increase in the number of entries to the target zone and the time spent in the target zone when compared to controls (E, 1F).', 'g001Chronic administration of zileuton ameliorates behavioral deficits of 3 Tg-AD mice.']",10.1371/journal.pone.0070991.g001,yes
PMC3989808,Figure_7,oa_package/46/5c/PMC3989808.tar.gz,['Late phase of the fundus fluorescein angiography of the right eye (A) and the left eye (B).'],Figure 7 Late phase of the fundus fluorescein angiography of the right eye (A) and the left eye (B).,yes
PMC2150681,Figure_2,oa_package/b7/f8/PMC2150681.tar.gz,"['High levels of GFP distributed to the RIS cytoplasm and the nucleus, whereas low levels were evident in the ROS cytoplasm ( A).', 'Little or no GFP-CT44 was detected in the RIS, either in the cytoplasm or the lateral plasma membrane ( B).', 'The expression pattern is essentially identical to that of GFP ( A) and suggests that both membrane association and a targeting/retention sequence are required for efficient and exclusive transport to the ROS.', 'f1""/>The cytoplasmic tail of rhodopsin can redirect GFP to the ROS.']","Figure 2 The cytoplasmic tail of rhodopsin can redirect GFP to the ROS. Confocal micrographs of transgenic retinas expressing GFP and GFP-CT44. Nuclei were stained with Hoescht 33342. (A) GFP distributed predominantly to the cytoplasm of the inner segment (is) and nucleoplasm. GFP resided at low levels in the outer segment (os) and was not associated with membranes. (B) GFP-CT44 localized almost exclusively to the outer segments. GFP (green) and Hoescht 33342 (blue). n, nucleus; m, mitochondria; and s, synaptic terminal. Bar, 5 m.",yes
PMC6078408,Figure_3,oa_package/49/ac/PMC6078408.tar.gz,['.'],"Fig. 3. (A) Longitudinal growth study shows no difference in zebrafish morphology. Three metrics were assessed in WT and zebrafish at 2, 3 and 4months post-fertilisation: length (blue) from the jaw to the anterior of the tail fin, height (green) at the anterior of the anal fin, and tail area (red) measured between the anterior of the anal fin and the anterior of the tail fin. No differences were seen between WT and zebrafish. (B) Representative transverse sections of 3-month-old zebrafish show muscle fibres in cross-section. A small number of very small fibres and fibres with centralised nuclei can be seen with similar frequency in both WT and fish (examples indicated by yellow arrows), indicating that these are not pathological changes. (C) Slow muscle distribution is preserved in the zebrafish. Immunofluorescence against the slow-muscle marker (s58) on transverse sections shows that slow muscle remains restricted laterally in the zebrafish. Image representative of sections from two WT and two fish at 6months post-fertilisation. As sections are derived from different positions on the anterior-posterior axis, slow-muscle area and cell number cannot be directly compared.",yes
PMC11558628,Figure_2,oa_package/7e/f5/PMC11558628.tar.gz,"[""Given the patient's altered sensorium, inability to reliably report chest pain, and the persistent elevation of troponin levels, coronary angiography was performed, which revealed no significant coronary artery lesions ()."", 'Coronary angiography revealing no significant lesions, showing left and right coronary arteries free of stenosis.', ':The diagnosis of reverse Takotsubo syndrome, characterized by basal segment hypokinesia rather than the typical apical ballooning, was considered.']","Fig. 2 Coronary angiography revealing no significant lesions, showing left and right coronary arteries free of stenosis.",yes
PMC9052064,Figure_3,oa_package/7a/c0/PMC9052064.tar.gz,"['Angiogram demonstrates aberrant left phrenic (arrow) arising from the left hepatic artery.', '']",Fig. 3 Angiogram demonstrates aberrant left phrenic (arrow) arising from the left hepatic artery.,yes
PMC8404122,Figure_7,oa_package/e1/32/PMC8404122.tar.gz,[],Figure-7 Postmortem findings of a sheep with unilateral hydronephrosis: (a) Notable dilated right ureter (black stars) and its corresponding kidney; (b) unilateral dilatation of the renal pelvis compared to a relatively normal left kidney.,yes
PMC10648581,Figure_1,oa_package/4d/8d/PMC10648581.tar.gz,"['Classification According to Histological SubtypesThe breakdown of the histological subtypes of the cases is shown in Table 2, and representative histological images of the different subtypes are shown in .', '2006-048616809439Histological images (Original magnifications, 60) from cases of (A) endometrioid carcinoma; (B) clear cell carcinoma; (C) serous carcinoma; and (D) de-differentiated carcinoma.']","Figure 1 Histological images (Original magnifications, 60) from cases of ( ) endometrioid carcinoma; ( ) clear cell carcinoma; ( ) serous carcinoma; and ( ) de-differentiated carcinoma.",yes
PMC4254453,Figure_9,oa_package/db/05/PMC4254453.tar.gz,"['g008""/>Optic nerveSimilarly as with retinal sections, the mHtt immunoreactivity in the optic nerve of R6/2 mice showed an increasing pattern from ages of 4 weeks to 12 weeks (A and B, respectively).', 'In contrast to the situation in the retina, mHtt was found in the GFAP-ir astrocytes of the optic nerve (D F).', 'g009Colocalization of GFAP (green) and mHtt (red) in the optic nerve.']",10.1371/journal.pone.0113317.g009,yes
PMC3810722,Figure_2,oa_package/a2/db/PMC3810722.tar.gz,"['In concert with decreased A generation, baicalein increased sAPP in a dose-dependent manner (A,B), suggesting that -secretase activity was promoted.', 'We noted that baicalein treatment significantly reduced the production of -CTF in the CHO/APPwt cell lysates, indicative of inhibition of the amyloidogenic pathway (C,D).', 'This finding was associated with the elevation of sAPP production in the cell media and reduced -CTF production in the cell lysate, indicating that baicalein promoted nonamyloidgenic APP processing in these cells (A D).']","Fig 2 Baicalein increases nonamyloidogenic APP cleavage in cultured cells. CHO/APPwt cells were treated with baicalein at 0, 2.5, 5, and 10 M as indicated for 12 hr. Secreted sAPP in the cell culture media were analyzed by sAPP ELISA (A) and WB (B), using 2B3 antibody. The sAPP ELISA results are represented as the mean SD of sAPP (pg/ml) in the cell culture media after baicalein treatment. Relative band density over vehicle control (1% DMSO in PBS; mean SD) was calculated by densitometry analysis as shown below the WB panel. These results are representative of three independent experiments, with n = 3 for each condition. C: Cell lysates were prepared, and APP CTFs were analyzed by WB using a rabbit polyclonal antibody against C-terminal APP (pAb751/770, C-APP). This -CTF band was further confirmed by the additional WB using 6E10 antibody (data not shown). D: Relative ratio (mean SD) of -CTF to -actin was calculated by densitometry analysis. The results are representative of three independent experiments, with n = 3 for each condition. One-way ANOVA followed by post hoc comparison revealed significant differences between 2.5, 5, or 10 M and 0 M baicalein treatment in both the increased sAPP and the decreased relative ratio of -CTF to -actin. * < 0.05, *** < 0.001, full-length APP, holo APP.",yes
PMC10882860,Figure_1,oa_package/70/fd/PMC10882860.tar.gz,"[' 1).', '', 'A Schematic for normal coronary artery origin and course off the aorta in relation to the pulmonary artery; B D represent short axis views of the aortic valve; B normal origin of the right coronary artery (RCA); C normal origin of the left main stem (LMS); D normal bifurcation of the LMS, into the left anterior descending (LAD) and circumflex (LCx) arteriesNomenclature of CAAsAllowing for a plethora of potential anomalies, CAAs can systematically be classified into anomalies of origin, anomalies of course and anomalies of termination.', ' 1, Tables 1 and 2) and this is particularly helpful in identifying high take off RCAs, which arise above the STJ.']","Fig.1 Normal coronary anatomy. Schematic for normal coronary artery origin and course off the aorta in relation to the pulmonary artery; represent short axis views of the aortic valve; normal origin of the right coronary artery (RCA); normal origin of the left main stem (LMS); normal bifurcation of the LMS, into the left anterior descending (LAD) and circumflex (LCx) arteries",yes
PMC8413894,Figure_4,oa_package/4a/76/PMC8413894.tar.gz,"['Firstly, we measured BACE1 endocytosis (10 min pulse) since Bin1-WT OE decreases transferrin endocytosis (59), which we confirmed (', ' 4Bin1 mutants impair the canonical transferrin endocytic recycling.', 'We found that Bin1-WT OE reduced transferrin internalization by 25% and that when cells overexpressed Bin1-PL and Bin1-KR, there was a similar reduction in transferrin endocytosis (20%) (', 'To follow transferrin recycling, we chased endocytosed transferrin for 20 min after a 10 min pulse and quantified the intensity remaining intracellularly (']","Figure4 Transferrin endocytic trafficking followed in N2a cells transiently expressing MYC (control), Bin1-WT, Bin1-PL, and -KR, using a pulse-chase assay with fluorescently labeled transferrin (Alexa647-transferrin), analyzed by epifluorescence microscopy. , endocytosed transferrin (2min Alexa647-transferrin, fire LUT) detected in N2a cells. Insets show MYC signal corresponding to MYC (control), Bin1-WT, Bin1-PL, and -KR. Scale bar, 10m. , quantification of endocytosed transferrin mean fluorescence in the percentage of control (n= 3, N = 109cells, N = 105cells, N = 101cells, N = 111cells; < 0.0001 Bin1-WT control, Bin1-PL control, Bin1-KR control, KruskalWallis test, mean SD). , nonrecycled transferrin (fire) detected upon 10min pulse with Alexa647-transferrin and 20min chase in N2a cells. Insets show MYC signal corresponding to control, Bin1-WT, Bin1-PL, and -KR. Scale bar, 10m. , quantification of non-recycled transferrin mean fluorescence in percentage of control (n= 3, N = 76, N = 97, N = 88, N = 97; = 0.8713 Bin1-WT control, > 0.9999 Bin1-KR control, = 0.0265 Bin1-WT Bin1-PL, = 0.0002 Bin1-PL control, < 0.0001 Bin1-PL Bin1-KR, KruskalWallis test, mean SD).",yes
PMC9489847,Figure_3,oa_package/76/73/PMC9489847.tar.gz,"['The normal thickness of the dura has been distorted due to a moderate expansion of fibrous connective tissue ().', 'Dural fibrosis; 20 Scale bar = 50 microns; Asterisk indicates area of fibrosis.']",Figure 3 Dural fibrosis; 20 Scale bar = 50 microns; Asterisk indicates area of fibrosis.,yes
PMC8752905,Figure_3,oa_package/bb/dd/PMC8752905.tar.gz,"['3"">Locoregional CAR T therapyDelivering immunotherapy directly into the tumor at the highest concentration may overcome problems with localization, infiltration, and systemic toxicity ( 3A).', 'Interventional oncology solutions for challenges in solid tumor immunotherapyImage-guided locoregional infusion of CAR T cells(A) Arteriogram showing selective intraarterial infusion of CAR T cells through a 2.', 'Therefore, priming the TME prior to infusion of CAR T cells can be another strategy to improve treatment outcomes ( 3B).', '24,119 This highlights the need for additional adjuvant strategies to support CAR T cells ( 3B).']","Figure2 Examples of minimally invasive, image-guided interventional oncology procedures performed with catheter-directed and percutaneous approaches (A) Arteriogram showing selective injection of Y90 microspheres in the anterior branch of the right hepatic artery. (B) Axial non-enhanced CT showing a microwave ablation of an apical left upper lobe nodule.",yes
PMC9527318,Figure_4,oa_package/41/ed/PMC9527318.tar.gz,"['We observed a significant loss of amplicon enrichment for the RelA/p65 motif in cells homozygous for the risk haplotype (A) but did not observe any significant difference in amplicon enrichment for the CEBPB motif (B).', 'To further explore the functional importance of the RelA/p65 and CEBPB binding motifs, we cloned several truncated sequences that omitted either the RelA/p65 or CEBPB binding motifs flanking the non-risk or risk alleles of rs10499197 into the minimal promoter luciferase vector (C).', 'When compared to the 337bp construct containing both motifs (D), loss of the RelA/p65 binding motif (330bp RelA or 150bp RelA) resulted in complete loss of enhancer activity when expressed in EBV B cells (s 4E,F), suggesting that RelA/p65 binding is necessary for enhancer activation.', 'In contrast, loss of only the CEBPB binding motif (203bp CEBPB) eliminated the allele-specific differences in luciferase expression (G).', 'This allele-specific effect was rescued by a luciferase construct that omitted the first N-terminal 70bp from the 337bp construct (267bp) and retained both the CEBPB and the RelA/p65 motifs (H).', 'For example, the enhancer effect is dependent on sequences flanking the RelA/p65 binding motif (E) but does not appear to be dependent on RelA/p65 based on our ChIP-qPCR experiments that showed a loss of RelA/p65 binding with the risk haplotype (A).', 'At the same time, the allele-specific effect is dependent on sequences flanking rs9494868 and the CEBPB binding motif (G and A), but CEBPB binding is not altered in an allele-specific manner by ChIP-qPCR (B).']","FIGURE 4 Enhancer function and allele-specific activity is dependent on sequences flanking the RelA/p65 and CEBPB binding motifs. ChIP-qPCR performed using EBV B cell lines homozygous for the non-risk or risk SLE haplotype (n = 7 cell lines per genotype) and antibodies specific against RelA/p65 , CEBPB , or rabbit IgG isotype control . qPCR reported as fold enrichment over isotype control (dotted line). Statistical comparisons were performed using Students t-test; * < 0.05 (Figure Abbreviations: NR, non-risk haplotype; R, risk haplotype). Schematic diagram of the truncated regions flanking rs10499197 that were cloned into the pGL4.23 luciferase vector. Dual luciferase assay using EBV B cells transiently transfected with empty pGL4.23 or pGL4.23 containing the indicated truncated regions in . Cells were stimulated without or with PMA/Ionomycin for 2h. Relative luciferase units were calculated as a normalized ratio of Firefly to Renilla; Students t-test, n > 3; * < 0.05; ** < 0.01; **** < 0.001. (Figure Abbreviations: V, pGL4.23 vector-only; NR, pGL4.23 with non-risk allele; R, pGL4.23 with risk allele; P/I, PMA/Ionomycin).",yes
PMC6274763,Figure_1,oa_package/80/23/PMC6274763.tar.gz,"['For each study group, we performed histological () and survival () analyses.', 'The absence of staining for Ki-67 indicates that cells are in a quiescent, non-proliferative stage (a).', 'As previously described [35,36], all [GFAP-tTA;TRE-SMOA1] mice develop large medulloblastoma tumors that can extend along the entire rostral-caudal length of the cerebellum, account for more than a quarter of the total cerebellar volume, express NeuN, indicative of neuronal origin, and show marked proliferative activity (positive for Ki67) (b).', 'Our observation that AMPK 2 KO impairs tumor progression in the SmoA1 mouse model of medulloblastoma directly verifies this hypothesis ( and ).', '200415537876Loss of AMPK impairs SHH medulloblastoma tumorigenesis in vivo.']","Figure 1 Loss of impairs SHH medulloblastoma tumorigenesis in vivo. Histopathological examination of the cerebellums of ( ) a Postnatal Day 63 (P63) mouse, ( ) a P56 [ ] mouse, ( ) a one-year-old [ ] mouse representative of the 7/17 (41%) [ ] mice that do not show any evidence of tumor upon histological analysis, and ( ) a P30 [ ] mouse representative of the 10/17 (59%) [ ] mice that show a tumor. The histological analysis includes H&E staining as well as Ki67 (marker of proliferation) and NeuN (marker of neuronal differentiation) immunohistochemistry (magnification: 4 and 40). The different cell layers of the cerebellum, i.e., the molecular (ML), Purkinje cell (PC), and internal granular cell (IGL) layers are labeled in the 40 control H&E picture. Scale bars: 200 m (4) or 25 m (40).",yes
PMC10319331,Figure_1,oa_package/25/ec/PMC10319331.tar.gz,"['Ulcers located in the upper extremity were only found in the multi ulcer group, and the only case of head and neck PG was found in the single ulcer group ().', 'A and B, Single ulcer and multi ulcer distributions.']",Fig 1 and Single ulcer and multi ulcer distributions. This includes the number of patients with ulcers located in the 5 body distributions along with the respective percentages.,yes
PMC3137450,Figure_1,oa_package/58/33/PMC3137450.tar.gz,"['Intraoral examination revealed an extensive mass involving the buccal mucosa ().', 'YamaguchiSNagasawaHSuzukiTFujiiEIwakiHTakagiMSarcomas of the oral and maxillofacial region: a review of 32 cases in 25 yearsClin Oral Investig200485255.']",Figure 1. Intraoral aspect showing extensive mass involving the buccal mucosa.,yes
PMC10647881,Figure_2,oa_package/be/65/PMC10647881.tar.gz,"['The microcatheter was flushed with 5% dextrose solution followed by an injection of N-butyl cyanoacrylate (TruFill; Codman and Shurtleff, Raynham, MA, United States) (NBCA) in a solution of ethiodized oil (Lipiodol, Guerbet, Villepinte, France) at a proportion of 1/2 ().', 'Thoracic duct embolization via percutaneous puncture of the cisterna chyli.']",Figure 2 Thoracic duct embolization via percutaneous puncture of the cisterna chyli. (A) Serial fluoroscopic imaging showing the contrast agent ascending to abdominal lymph nodes; (B) Fluoroscopic imaging showing the cisterna chyli (white arrow); (C) Successful catheterization into the thoracic duct; (D) Fluoroscopic imaging following coil embolization (white arrow) and glue embolization (black arrow).,yes
PMC7110585,Figure_3,oa_package/5c/83/PMC7110585.tar.gz,"['Nevertheless, wide surgical resection has been recommended, although the procedure may necessitate partial or total penile resection (', '21\n']","Fig.3 Preputial tumor on a ferret ( ), prepped for surgical removal.",yes
PMC11451770,Figure_2,oa_package/e3/54/PMC11451770.tar.gz,"['[7] described the presence of dilated vein coursing from the deeper part of JRCH into the inner retina and communicating with a retinal vein, possibly indicating a draining vessel on OCTA [].', 'IJO_29_24-F2"">(a) Color fundus photograph of the right eye showing peripapillary RCH.']","Figure 2 (a) Color fundus photograph of the right eye showing peripapillary RCH. (b) En face OCTA showing compact vascularity of the tumor. (c) Cross-sectional OCTA showing flow signal within the tumor. OCTA = optical coherence tomography angiography, RCH = retinal capillary hemangioblastoma",yes
PMC7526458,Figure_7,oa_package/d5/59/PMC7526458.tar.gz,"['As depicted in A, IL-10 (10 ng/mL) or IL-10 plus Hb (1 M) significantly increased CD163 expression in macrophages.', 'Next, induction of HO-1 was also observed when differentiated THP-1 cells were treated with IL-10 and a synergistic increase in HO-1 was seen with IL-10 plus Hb cotreatment under hyperglycemic conditions (B).', 'IL-10 induced CD163 and HO-1 expression in THP-1 macrophages cultured under hyperglycemia.']","Figure 7 IL-10 induced CD163 and HO-1 expression in THP-1 macrophages cultured under hyperglycemia. THP-1 cells were differentiated to macrophages and cultured under high glucose conditions (25 mM). ( and ) Cells were treated as described in Methods, and CD163 ( ) and HO-1 ( ) protein expression were measured by Western blots. GAPDH was used as a loading control. Top: representative blots. Bottom: quantitative data. One-way ANOVA, followed by Tukeys test; = 3 per group, * < 0.05 vs. control; < 0.05 vs. Hb; < 0.05 vs. IL10.",yes
PMC6054937,Figure_4,oa_package/30/90/PMC6054937.tar.gz,['Detection of residual endo- -N-acetylglucosaminidase (EndoS) in the GST-EndoS treated IgG after protein G purification.'],"Figure 4 Detection of residual endo-- -acetylglucosaminidase (EndoS) in the GST-EndoS treated IgG after protein G purification. M2139 antibody (2mg) was incubated with 0.1g or 1g of EndoS in PBS at 37C for 1h followed by immediate purification on HiTrap Protein G HP column Chromatogram. The flow through fraction and the eluate were analyzed by SDS-PAGE followed by Western blot. SDS-PAGE and Western blot using alkaline phosphatase or peroxidase conjugate as secondary antibody. Flow-through samples were concentrated before loading on to SDS-PAGE. After transfer, the Western blot membrane was incubated with Eu-N1-labeled anti-GST antibody, followed by donkey anti-goat AP or anti-goat HRP conjugate. For the AP conjugate, the positive bands were visualized using 1-Step TM NBT/BCIP substrate. For the HRP conjugate, the light emission was enhanced using ECL followed by film exposure. Lane 1, EndoS; lane 2, M2139; lane 3, concentrated flow through (0.1mg group); lane 4, eluate (0.1mg group); lane 5, concentrated flow through (1mg group), lane 6, eluate (1mg group); lane 7, protein standard; lane 8, control GST-fusion protein.",yes
PMC4414289,Figure_3,oa_package/2a/11/PMC4414289.tar.gz,"['As shown in s 3A to D, MSC treatment significantly alleviated chronic inflammation with improved clinical scores.', 'Expectedly, these colitis characteristics were reversed after MSC treatment (s 3E and F, P 0.', 'MSCs protect mice against the development of DSS-induced colitis on day 33.', 'MSCs inhibit inflammatory cytokines in colon and serumCytokine levels are critical evidence to show the effect of immune cells on CAC growth.']","Figure 3 MSCs protect mice against the development of DSS-induced colitis on day 33. Colons were excised for macroscopic observation and assessment of colonic length The changes in body weight were calculated based on initial body weight. The clinical disease score of colitis, which includes stool status, fecal blood, length of colon and weight loss, was used to evaluate the therapeutic effect of MSCs. Colon sections from mice with different treatments were examined using H & E staining. Histology scores were derived from microscopic analysis of longitudinal colon sections from each mouse. Values are expressed as meansSEM. * <0.05, or ** <0.01. DSS, dextran sulfate sodium; MSCs mesenchymal stem cells; SEM, standard error of the mean.",yes
PMC5504859,Figure_2,oa_package/39/a6/PMC5504859.tar.gz,"[' 2).', 'c The titanium clip (arrow) can be viewed macroscopically after dissecting the dyed SLN\nTable 2The LDPs defined by CEUS and actual LDPsa\nLDPCEUSActual LDPBlue dyeSSLC242121PSLC333SSLC + PSLC888SSLC + DSLC71010SSLC + PSLC + DSLC444\naActual LDPs: defined basing on both CUES and blue dye results\nLive dual images and macroscopic appearance of a bifurcated SLC.']","Fig. 2 Gray-scale ultrasound examination, X-ray examination, and dissection used to confirm the insertion of a titanium clip into the SLN. The titanium clip ( ) was shown with high-echo on gray-scale ultrasound. The titanium clip ( ) was shown with high density on X-ray imaging. The titanium clip ( ) can be viewed macroscopically after dissecting the dyed SLN",yes
PMC5575325,Figure_4,oa_package/82/0a/PMC5575325.tar.gz,"[' 4a).', 'Polarization of RPE cells results in upregulation of ferritin and -cleavage of PrPC.', '\nBiochemical evaluation of lysates prepared from non-polarized (NP) and polarized (P) cells revealed a robust increase in ferritin expression by ~10-fold upon polarization (', ' 4b, lanes 1 vs.', ' 4c).', ' 4b, lanes 5 vs.', ' 4b, lanes 9 12; ', ' 4c).', ' 4b, lanes 7 8 vs.', ' 4d, lanes 1 2), confirming that this observation is not an artifact of the cell line.', ' 4d, lanes 3 4).', ' 4 above.', ' 4a) reveals undetectable levels of C2 in M17 cells even after over-expressing PrPC (lanes 1 2).', ' 4a) shows FL and C1 in M17 and brain lysates (lanes 8, 9, 14), and mainly C2 in RPE samples (lanes 10 13).']","Figure 4 Polarization of RPE cells results in upregulation of ferritin and -cleavage of PrP . ( ) Schematic representation of full length (FL), -cleaved (C1), and -cleaved (C2) forms of PrP and antibody reactivity against 3F4 and anti-C (8H4) epitopes. ( ) Probing of lysates from non-transfected and PrP expressing RPE19 cells with 3F4 shows cleavage of PrP at the -site following polarization, resulting in the generation of C2 (lanes 1 & 3 vs. 2 & 4). The change in the ratio of full-length (FL) vs. -cleaved (C2) form of PrP is more obvious in deglycosylated samples (lanes 58 vs. 912). Re-probing for ferritin shows upregulation in PrP -expressing cells relative to vector controls (lanes 5 & 7 vs. 6 & 8), and a dramatic increase in both cell lines following polarization (lanes 1 & 3 vs. 2 & 4, lanes 58 vs. 912). NP: non-polarized; P: polarized. Densitometric analysis of protein bands following normalization with -actin shows ~10 fold increase in ferritin following polarization in both vector and PrP -expressing cells. Values are meanSEM of the indicated n. ***p<0.001. ( ) Probing of neuroretinal lysates from PrP (TgHu) mice and human brain for PrP shows -cleavage of ~70% of PrP in the neuroretina relative to ~10% in brain samples.",yes
PMC5639180,Figure_1,oa_package/34/2e/PMC5639180.tar.gz,"['5 cm (A).', '5 cm with U shaped bend at its tip (B).', 'Root of mesentery also extended up to cecum and ascending colon attaching it with posterior abdominal wall (B).', ""1StandringSGray's anatomy: the anatomical basis of clinical practice41st edNew YorkElsevier Churchill Livingstone20162MooreKLPersaudTVTorchiaMGThe developing human: clinically oriented embryology9th edPhiladelphia, PASaunders20133SadlerTWLangman's medical embryology13th edPhiladelphia, PAWolters Kluwer20164PickhardtPJBhallaSIntestinal malrotation in adolescents and adults: spectrum of clinical and imaging featuresAJR Am J Roentgenol200217914291435124380315SchoenwolfGCBleylSBBrauerPRFrancis-WestPHLarsen's human embryology4th edPhiladelphia, PAElsevier Churchill Livingstone20096MooreKLAgurAMDalleyAFClinically oriented anatomy7th edPhiladelphia, PAWolters Kluwer20147NorrisJMOwusuDAbdullahAARajaratnamKSubhepatic caecal tumour in an adult with intestinal malrotationBMJ Case Rep20142014bcr20142071638NayakSBGeorgeBMMishraSSurendranSShettyPShettySDSessile ileum, subhepatic cecum, and uncinate appendix that might lead to a diagnostic dilemmaAnat Cell Biol201346296298243866039BrunicardiFCAndersenDKBilliarTRDunnDLHunterJGMatthewsJBPollockRESchwartz's principles of surgery10th edNew YorkMcGraw-Hill Medical201510MVNGireeshSubhashLPPaiVSub-hepatic caecumJ Clin Diagn Res201371992199324179918(A) Photograph of subhepatic cecum: white line drawn at the level of ileocolic junction.""]","Fig. 1 (A) Photograph of subhepatic cecum: white line drawn at the level of ileocolic junction. Cecum in subhepatic location. TI A is placed intraperitoneally. (B) Subhepatic cecum is pulled up to show appendix with mesenteric extension. White line drawn at the level of ileocolic junction showing conical cecum and appendix in 12'O clock position. A, full length of vermiform appendix arising from conical cecum; C, cecum; FL, falciform ligament; J, jejunum; LL, left lobe of liver; MC, mesentric attachment of cecum; MI, mesentric extension of ileum terminal ascending part; RL, right lobe of liver; SAC, short ascending colon; TC, transverse colon; TI, terminal ileum; TI A, ascending part of terminal ileum.",yes
PMC5473003,Figure_1,oa_package/93/c8/PMC5473003.tar.gz,"['1a).', '1b).', '1c).', 'Olig001-GFP expression in rats.', '0001 Fisher s PLSD]\nMorphologically, the expression pattern of GFP+ cells appeared to be specific to oligodendrocytes, consistent with the oligodendroglial-specific tropism of Olig001, as the GFP signal was strong in the oligo-rich white matter of the corpus callosum, with distinct labeling of myelin in the patch component of the striatal patch-matrix (', '1a).', '1d top two panels).', '1d third panel), and virtually no transduction of astrocytes, with only 0.', '1d bottom panel).', '1e for each cellular phenotype [effect of phenotype F(2,9) = 11,711.']","Fig. 1 Olig001-GFP expression in rats. Representative image of GFP expression in the striatum and corpus callosum of rats 4-weeks following injection of Olig001-GFP. Robust GFP expression is seen in the white matter bundles in the striatum and corpus callosum ( =4). 100m. Stereological quantification estimated 126,57411,303.77 GFP+ cells, which occupied a volume of 3.8730.304mm in the striatum. GFP expression is seen to colocalize with oligodendroglia marker Olig2 in the corpus callosum (top panel) and striatum (second panel). Very little GFP expression is seen to colocalize with neuronal marker NeuN (third panel) or astrocyte marker GFAP (bottom panel). bar 50m. 9497% of GFP+ cells in the corpus callosum and striatum co-localized with oligodendrocyte marker Olig2, only 2.94.7% of GFP+ cells co-localizing with neuronal marker NeuN, 0.180.49% of GFP+ cells co-localizing with astrocyte marker GFAP phenotype [effect of phenotype F(2,9)=11,711.6, <0.0001, oligodendrocytes vs. astrocytes <0.0001 Fishers PLSD post hoc, oligodendrocytes vs. neurons, <0.0001, Fishers PLSD, astrocytes vs. neurons =0.0001 Fishers PLSD]",yes
PMC10667534,Figure_4,oa_package/43/be/PMC10667534.tar.gz,"[' 4a).', ' 4b).', ' 4c and d).', ' 4c).', ' 4d).', 'Effect of HIF-1 stabilization on LDHA, PDK1, and MCT4 protein expression.', 'HIF-1 stabilization and oxidative phosphorylationGiven that HIF-1 impairs OXPHOS by downregulating electron transport chain proteins and promoting mitophagy, we measured cytochrome c oxidase and succinate dehydrogenase (SDH-A/Complex II) protein and citrate synthase enzyme activity.']","Figure 4 Effect of HIF-1 stabilization on LDHA, PDK1, and MCT4 protein expression. ( ) LDHA protein was significantly reduced (F(2,8)=38.55, p<0.0001) in 4-week Roxa retina compared to control and 2-week retinas. Tukey's multiple comparisons test showed a significant reduction in LDHA in 2- week (p=0.0006) and 4-week Roxa (p<0.0001) retinas compared to control retinas. ( ) PDK1 protein was significantly higher (F(2,9)=42.73, p<0.0001) in 4-week Roxa retina compared to control and 2-week retinas. Tukey's multiple comparisons test showed a significant PDK1 increase in 4weeks compared to control (p=0.0004) and 2-week Roxa (p<0.0001) retinas. ( ) MCT4 protein was significantly lower (F(2,9)=13.64, p=0.0019) in 4-week Roxa retina compared to control and 2-week retinas. Tukey's multiple comparisons test showed MCT4 decreased in 4weeks compared to control (p=0.0064) and 2-week Roxa (p=0.0023) retinas. ( ) Representative immunofluorescence images showing MCT-4 (magenta) and GLAST (green) expression in retina cross-sections for the Control, Roxa 2-week, and Roxa 4-week groups. Scale bar=20m. GCL=ganglion cell layer; INL=inner nuclear layer. Proteins were analyzed by capillary electrophoresis and normalized to total protein levels. Values shown are meanSEM of .",yes
PMC3909843,Figure_2,oa_package/f4/b7/PMC3909843.tar.gz,"['Of the 16 DCIS on 14-G semi-automated CNB, 14 were confirmed to be DCIS on final pathology ().', 'DCIS = ductal carcinoma in situ, IDC = invasive ductal carcinoma, US = ultrasound, ADH = atypical ductal hyperplasia, DIN = ductal intraepithelial neoplasia, CCH = columnar cell hyperplasia, FCC = fibrocystic changesDetails from case of 54-year-old asymptomatic woman.']","Fig. 2 Details from case of 54-year-old asymptomatic woman. Mammography shows clustered fine pleomorphic calcifications (arrows). BI-RADS assessment is category 5. Targeted US shows multiple echogenic dots (arrows) within irregular indistinct isoechoic mass. Specimen radiography after US-guided 14-G semi-automated CNB shows multiple microcalcifications (arrows) in five of six cores retrieved. Pathology results of both CNB and final surgery were DCIS. BI-RADS = Breast Imaging-Reporting and Data System, US = ultrasound, CNB = core needle biopsy, DCIS = ductal carcinoma",yes
PMC5122797,Figure_2,oa_package/79/54/PMC5122797.tar.gz,"['Unenhanced measurements 30 HU usually denote phaeochromocytoma or adrenocortical carcinoma ().', 'Heterogeneous enhancement in a patient with a right adrenal mass 1 min after i.']","Figure 2 Heterogeneous enhancement in a patient with a right adrenal mass 1min after i.v. contrast. Unenhanced images measured at 36HU, at 15min enhancement was 98HU. CT findings highly suggestive of adrenocortical carcinoma, pathologically confirmed after adrenalectomy.",yes
PMC9924910,Figure_4,oa_package/bf/6b/PMC9924910.tar.gz,"['57), A and B and Supplementary Table 2].', '\nGroup comparisons of fixel metrics.', '49), C and D and Supplementary Table 3].']",Figure 4 . ( ) Difference in fixel metrics between age-matched healthy controls (HC) and CADASIL patients in the SVD sample quantified with Cohens represented by colour. Circle size depicts statistical significance level. ( ) Violin plots of fixel metrics of four representative fibre tracts in the SVD sample for exemplary illustration. ( ) Difference in fixel metrics between age-matched AT and A+T; AT and A+T+; A+T and A+T+ quantified with Cohens represented by colour. Circle size depicts statistical significance level. ( ) Violin plots of fixel metrics of the same four tracts in the Alzheimers disease sample. Refer to for abbreviations of the fibre tracts.,yes
PMC10145941,Figure_8,oa_package/37/c3/PMC10145941.tar.gz,"['By immunoblotting we observed a time-dependent upregulation of both cyclic GMP-AMP synthase (cGAS)/stimulator of IFN genes (STING) and retinoic acid inducible gene I (RIG-I)/mitochondrial antiviral signaling protein (MAVS) after TAC (Supplemental ).', 'To verify that ISG15 associates with filamin-C in cardiomyocytes, we performed coimmunoprecipitation experiments in which IFN- caused ISG15 to coimmunoprecipitate with filamin-C, with a reduction in this association following ISG15 knockdown (A).', 'Lastly, using dual fluorescence immunostaining, we observed that ISG15 colocalized with filamin-C at, or close to, cardiomyocyte intercalated discs in the hearts of mice after TAC (B) and humans with NICM (C), with an expected absence of ISG15 colocalizing with filamin-C in Isg15 / mice (Supplemental 2).', 'ISG15 associates with filamin-C in mouse and human hearts.']",Figure 8 ISG15 associates with filamin-C in mouse and human hearts. ( ) Immunoprecipitation for filamin-C and immunoblotting for ISG15 in human cardiomyocytes following knockdown of ISG15 with siRNA and incubation with 500 IU/mL IFN- for 48 hours ( = 3 per condition). ( and ) Dual immunofluorescence staining for ISG15 and filamin-C in the hearts of sham-operated mice and mice 1 week after TAC ( ) and human control tissue and LV tissue from a human with NICM ( ). Scale bars: 10 m. Values are mean SD. * < 0.05 by 1-way ANOVA followed by Dunnetts post hoc test.,yes
PMC7568110,Figure_7,oa_package/0c/62/PMC7568110.tar.gz,"['However, a correlative CT scan image clearly showed that the bony lesion was an incidental vertebral body hemangioma [].', ':Sagittal postcontrast T1W magnetic resonance imaging (MRI) of the thoracic spine (a) shows an enhancing epidural soft-tissue lesion nearly filling the spinal canal (arrow) with dural tails superiorly and inferiorly, which appears hypointense on T2W (b) and T1W (c) MRI at T4 vertebral level.']","Figure 7: Sagittal postcontrast T1W magnetic resonance imaging (MRI) of the thoracic spine (a) shows an enhancing epidural soft-tissue lesion nearly filling the spinal canal (arrow) with dural tails superiorly and inferiorly, which appears hypointense on T2W (b) and T1W (c) MRI at T4 vertebral level. Axial postcontrast MRI (d) shows severe compression and displacement of the spinal cord to the left anterolateral aspect (arrowhead) by the enhancing lesion (arrow). Axial T2W MRI (e) shows hypointensity of the lesion filling the canal (arrow) and no hyperintense fluid signal of surrounding CSF is seen as it is obliterated. All of the above MR sequences show a slightly T1 and T2 hyperintense, enhancing lesion within the T4 vertebral body (thick arrow), which could introduce the differential diagnosis of vertebral bony and spinal canal metastatic components. Axial bone window computerized tomography scan (f) image, however, clearly shows that the bony lesion is just an incidental vertebral body hemangioma (thick arrow) with characteristic polka-dot appearance of its vertical striations.",yes
PMC8007996,Figure_2,oa_package/9a/b8/PMC8007996.tar.gz,['Multiphase contrasted CT imaging was performed with prior administration of oral contrast ().'],"FIGURE 2 (a) Axial and (b) sagittal portal venous imaging of the abdomen, demonstrating jejunal loops within the left paraduodenal hernia (black arrow) and stretched mesenteric vessels (white arrow) entering the hernial sac. Dilatation of the proximal duodenum (star) is seen with non-visualisation of a normal duodenal jejunal flexure. Note the position of the left paraduodenal hernia, approximating the posterior margin of the stomach.",yes
PMC9422278,Figure_1,oa_package/6f/5b/PMC9422278.tar.gz,"['Urgent computed tomography (CT) scan showed dilated small bowel loops with a whirl sign ().', 'Abdominal CT scan in the sagittal plane showing the whirl sign (arrow).', 'After brief resuscitation, surgery was performed by a 5-year-experience surgeon.']",Fig. 1 Abdominal CT scan in the sagittal plane showing the whirl sign (arrow).,yes
PMC9283810,Figure_3,oa_package/dd/47/PMC9283810.tar.gz,[],Figure 3 Aspect papillomateux du voile du palais Papillomatous appearance of the soft palate,yes
PMC5377291,Figure_3,oa_package/69/10/PMC5377291.tar.gz,"['There was no evidence of tuberculosis or candidiasis at perforation site (', 'We propose our entity as pseudo-diverticula as our diverticulum did not have a muscle coat and it was totally devoid of muscularis propria (', ' 2 3Histopathology showing complete lack or absence of muscle fibers at the site of perforation confirming pseudo-diverticulum.']",Figure3 Histopathology showing complete lack or absence of muscle fibers at the site of perforation confirming pseudo-diverticulum.,yes
PMC9299925,Figure_3,oa_package/29/ba/PMC9299925.tar.gz,"['Hematoxylin and eosin staining of traditional incisional biopsy (a) reaching the muscularis fascia and muscular tissue dermal, where eosinophils are visible at higher magnification; microbiopsy obtained with an 18G ( 2 mm inner diameter) needle (b)Ultrasound mapping realized by 50 MHz and 15 MHz of three repere points (abdomen, Ab; forearm, F; and thigh, T) was performed in a 45 year old patient with eosinophilic fasciitis at baseline (a) and 3 months post UVA 1 adjuvant phototherapy (t\n5) (b): the thickness of the muscularis fascia (MF) is significantly reduced at t\n5 in all repere points, whereas the dermis (D) and hypodermis (HD) is morphologically unalteredUltrasound mapping realized by 15 MHz of two repere points (forearm, F and thigh, T) performed in a 40 year old patient at baseline (a, c) and 3 months post UVA 1 adjuvant phototherapy (t\n5) (b, d): the thickness of the muscularis fascia is significantly reduced at both F and T repere pointSummary of the t\n0 t\n7 assessments of average dermal value, hypodermal, and muscularis fascia thickness by high frequency ultrasound (a), Localized Scleroderma Cutaneous Assessment Tool (LoScat) (b), and of the Dermatology Life Quality Index (DLQI) (c)Cell culture imagingHealthy control human fibroblasts in P1, day 14 of culture, had a cell density of ~1,000,000 cells/flask 25 cm2 on average, 45 10 nm short transversal diameter, and 500 50 nm longitudinal diameter.']","Figure 3 Ultrasound mapping realized by 50MHz and 15MHz of three repere points (abdomen, Ab; forearm, F; and thigh, T) was performed in a 45yearold patient with eosinophilic fasciitis at baseline (a) and 3months postUVA1 adjuvant phototherapy ( ) (b): the thickness of the muscularis fascia (MF) is significantly reduced at in all repere points, whereas the dermis (D) and hypodermis (HD) is morphologically unaltered",yes
PMC10501244,Figure_1,oa_package/8a/b6/PMC10501244.tar.gz,[],"FIGURE 1 Overall survival with a minimum followup period of 19.4months (KaplanMeier plot). CI, confidence interval; HR, hazard ratio; OS, overall survival; mo, months.",yes
PMC7068097,Figure_1,oa_package/de/81/PMC7068097.tar.gz,"['T2 weighted (T2W) and fluid-attenuated inversion-recovery (FLAIR) images showed the symmetric hyperintensity of the dentate nuclei and of the cerebellar white matter with some associated calcium deposits in the adjacent zone ().', '.']","Figure 1. Axial W MRI of the brain shows bilateral hyperintense lesions involving the dentate nuclei (arrows) and the deep cerebellar white matter. The brain stem appears normal in signal intensity. W, weighted.",yes
PMC6098145,Figure_5,oa_package/43/5c/PMC6098145.tar.gz,"[' 5A).', 'Klebsiella oxytoca hampers gut barrier function in cachectic mice.', 'When cachectic mice were administered K.', ' 5B).', ' 5C).', ' 5D F).', ' 5G).']","Figure 5 hampers gut barrier function in cachectic mice. ( ) Fecal . levels in healthy mice receiving the vehicle (CT), in C26-transplanted mice receiving the vehicle (C26) and in C26-transplanted mice receiving . in their drinking bottle (C26-Kox). ( ) In black, number of mice for which coliform bacteria were detected in their mesenteric lymph nodes (MLN). ( ) Fecal . levels in mice displaying coliform bacteria in their MLN and in mice free of coliform bacteria in their MLN, within the C26-Kox group. ( ) Ileal mRNA expression levels of key markers involved in gut permeability, epithelium renewal, mucosal immunity and antimicrobial peptide production. Data are presented as whiskers plots with minimal and maximal values (A) or meanSEM ( ). The dotted line indicates the expression level in sham-injected mice. N=78. p<0.1; *p<0.05; **p<0.01; ***p<0.001.",yes
PMC4392949,Figure_3,oa_package/1a/1f/PMC4392949.tar.gz,"['In 1 specimen, 2 small accessory fissures resulted in 2 small accessory lobes present close to the base of gall bladder near the inferior border ().', '002""/>Shows accessory lobes present close to the base of gall bladder near the inferior border on inferior surface of right lobe of liver.']",Figure 3 Shows accessory lobes present close to the base of gall bladder near the inferior border on inferior surface of right lobe of liver.,yes
PMC9242822,Figure_6,oa_package/76/5d/PMC9242822.tar.gz,"['As shown in , progesterone receptor (PR) staining was positive in 30% of tumor cells, which was a lower percentage of tumor cells compared with ER (a); Ki-67 proliferative index was positive in 20-30% (magnification 100; (b)).', '005"" position=""float""/>Immunohistochemistry staining of male breast cancer.']","Figure 6 Immunohistochemistry staining of male breast cancer. (a) Progesterone receptor staining was positive in 30% of tumor cells (lower percentage compared to estrogen receptor); magnification, 200. (b) The Ki-67 proliferative index was 20-30%; magnification, 100. (c) HER-2 was completely negative; magnification, 100.",yes
PMC7116793,Figure_1,oa_package/c3/1d/PMC7116793.tar.gz,"['As indicated in A, parallel sections were then stained sequentially with either H E, with three different antibodies (Ki67, cPARP, CK) using IHC or with the same three antibodies combined on one section using mIF.', 'By contrast, mIF allows for easy detection of multiple markers on one section, generating a merged image that integrates the staining for all biomarkers (A).', 'Representative FoVs for both vehicle and cisplatin-treated PDEs with the three approaches are shown in B and C.', 'Slides stained by IHC for the three biomarkers (Ki67, cPARP, CK) were digitised using a whole slide scanner at 40x magnification () and then analysed using three available software packages: QuPath, ImmunoRatio and VisioPharm.', 'There was a significant linear relationship in positive counts using both platforms (Supplementary Ai).', 'When expressed as positive cells/mm2, Platform-3 counted more positive cells than Platform-1 (Supplementary Aii).', 'The increase in cell density per mm2 observed with Platform-3 was statistically proportionally different to Platform-1, and this method of analyses also consistently counted ~39% more cells using Platform-3 (Supplementary Aiii).', 'To determine which Platform detected positive cells closest to the ground truth , eight FoVs were selected and positive cells were identified by Platform-1 and Platform-3 and compared to cells manually counted by a histomorphometrist (Supplementary B).', ' 12 m (A).', 'Immunostaining of NSCLC PDEs\na Serial sections and sequential staining of parallel sections with: H E, Ki67 using IHC, CK using IHC, cPARP using IHC and all three biomarkers together with DAPI by mIF.']","Figure 1 Immunostaining of NSCLC PDEs Serial sections and sequential staining of parallel sections with: H&E, Ki67 using IHC, CK using IHC, cPARP using IHC and all three biomarkers together with DAPI by mIF. Serial sections from vehicle (b) or cisplatin (c) treated NSCLC PDEs of the lung adenocarcinoma subtype were stained with H&E, with the three biomarkers (Ki67, CK, cPARP) on sequential sections using IHC, or with the three biomarkers on the same section using mIF. The merged image generated from mIF shows integration of Ki67 (green), CK (yellow), cPARP (red) with DAPI (blue) staining.",yes
PMC8422410,Figure_1,oa_package/b9/55/PMC8422410.tar.gz,"['Finally, we present the case of a 43-year-old male patient who had previously undergone an epididymal cyst 18 years earlier and a right parasagittal parietal hemangioblastoma 12 years ago [].', ':T1 sequenced MRI with paramagnetic contrast showing a right parietal tumor lesion with a peritumoral cyst in a coronal section.']",Figure 1: T1 sequenced MRI with paramagnetic contrast showing a right parietal tumor lesion with a peritumoral cyst in a coronal section.,yes
PMC10686880,Figure_8,oa_package/3c/b7/PMC10686880.tar.gz,['Leriche syndrome: The three dimensional CT rendering technique (VRT) of the abdomen with aortic angiography and lower extremity showing atherocalcific changes of bilateral external iliac arteries (blue arrows) and non-visualization of infrarenal abdominal aorta with establishment of collaterals (yellow arrows).'],Figure 8 Leriche syndrome: Contrast enhanced CT arterial phase showing non contrast opacification of right common iliac artery (blue arrow) and partial contrast opacification of left common iliac artery (yellow arrow).,yes
PMC8281787,Figure_5,oa_package/1b/5a/PMC8281787.tar.gz,['MALT: mucosa-associated lymphoid tissueImmunohistochemistry (20x objective lens) for kappa (left) and lambda (right) with brown-stained cells consistent with positive stain uptake.'],Figure 5 Immunohistochemistry (20x objective lens) for kappa (left) and lambda (right) with brown-stained cells consistent with positive stain uptake. There is a small population of kappa-restricted cells (black arrow on example positive cell on left). No significant uptake of lambda stain on the right.,yes
PMC6106831,Figure_3,oa_package/6e/80/PMC6106831.tar.gz,"[' 3).', '3a) repeat the same pattern that has been seen throughout the analysis: induction by infection over low levels of expression in uninfected tissue that is much greater in magnitude in diestrus phase.', 'Leading edge genes from top phase:infection interaction-effect gene sets.', 'N = 4 per condition except PBS estrus N = 3In contrast to the cytokine gene set, most of the leading-edge genes in the interferon alpha/beta signaling gene set (Reactome pathway R-HSA-909733) (', '3b) displayed very little infection-dependent induction in estrus, but robust diestrus-phase induction by infection.', '3a).', '3).']","Fig. 3 Leading edge genes from top phase:infection interaction-effect gene sets. Transcript levels of leading edge genes, from GSEA identified top phase:infection interaction effect gene sets ( Chemokine activity gene set [GO:0008009], Interferon alpha/beta signaling pathway [R-HSA-909733]), as measured 6h after infection, are displayed by heatmap. Rows represent genes, with log2(expression) values z-normalized (to a mean of zero and a standard deviation of one) across all samples. Colors are scaled so that red and blue indicate z-scores of 2 or2, respectively, and white indicates a z-score of 0 (row-wise mean). Rows are arranged in descending order from top to bottom by phase:infection statistic. N=4 per condition except PBS estrus N=3",yes
PMC8318065,Figure_6,oa_package/d4/49/PMC8318065.tar.gz,"['5, 1, 2, and 4 M], and the results from the MTT assay indicate that DOX at the doses of 2 and 4 M strongly suppressed viability of H9c2 cells (A, P 0.', 'The MDA content of H9c2 cells was also induced by DOX at 2 and 4 M (B, P 0.', 'Intracellular Fe2+ was induced by DOX at 1, 2 and 4 M (C, P 0.', 'Moreover, it could be found that zVAD, which is an apoptosis inhibitor, had no protective effect on the viability of DOX-treated H9c2 cells (D).', 'Effects of HMGB1 and DXZ on DOX-treated injury of H9c2 rat cardiomyocytes.']","Figure 6 Effects of HMGB1 and DXZ on DOX-treated injury of H9c2 rat cardiomyocytes. The viability of H9c2 cells after the incubation of DOX for 12 h at 0 (control), 0.5, 1, 2, and 4 mol/L was detected by MTT assay. The levels of MDA in H9c2 cells after the incubation of DOX for 12 h at 0 (control), 0.5, 1, 2, and 4 mol/L were detected by a commercial kit. The Fe content of H9c2 cells after the incubation of DOX for 12 h at 0 (control), 0.5, 1, 2, and 4 mol/L was detected by assay kits. The viability of H9c2 cells after drug treatment in the control group or DOX-treated cells after incubation of FER-1 (10 mol/L), zVAD (20 mol/L, an apoptosis inhibitor), or DXZ (100 mol/L) for 72 h was detected by MTT assay. The MDA levels in H9c2 cells after drug treatment for 24 h were detected by a commercial kit. The LDH levels in H9c2 cells after drug treatment for 24 h were detected by a commercial kit. ** < 0.01, *** < 0.001 vs. control. < 0.001 vs. DOX. Each experiment was repeated three times.",yes
PMC6251292,Figure_1,oa_package/6a/8d/PMC6251292.tar.gz,"['Lingula with a wide base and narrow rounded or pointed apex were classified as triangular [] and the lingula with the quadrangular top as truncated [].', 'The entire lingula except for its apex merged into the ramus is nodular [] and in assimilated type [] the lingula becomes completely incorporated into the ramus.', 'Different shape of linguleAccessory mandibular foramenRESULTSThe distribution of different shape of lingule and AMF in male and female dry mandibles is shown in Table 1 and Chart 1.']",Figure 1 Different shape of lingule,yes
PMC7415959,Figure_1,oa_package/25/11/PMC7415959.tar.gz,[],"FIGURE 1 RAGE pharmacological inhibition prevented motor neuron death induced by hSOD1 astrocytes and hSOD1 lumbar spinal cord extracts. A, Nontransgenic embryonic motor neurons were plated on top of astrocytes isolated from nontransgenic (NTG) or hSOD1 mice. Vehicle (Control, Ctrl) or RAGE inhibitors, FPSZM1 (100nmol/L; ZM1) and RAP (20mol/L), were added 2h after motor neuron plating. Neuronal survival was determined 72h later. Data are expressed as percentage of motor neuron survival on top of NTG control astrocytes (meanSD). *Significantly different from NTG control ( .05); Significantly different from hSOD1 control ( .05). B, Primary motor neuron cultures isolated from the spinal cord of E12.5 hSOD1 embryos were maintained with GDNF (1ng/mL). Two hours after plating, cultures were treated with vehicle (control GDNF) or spinal cord extracts (0.5g protein/mL) from early symptomatic hSOD1 mice or agedmatched nontransgenic littermates (NTG). Vehicle (Ctrl) or RAGE pharmacological inhibitors, FPSZM1 (100nmol/L; ZM1) and RAP (10mol/L), were added 30min before treatment with spinal cord extracts and motor neuron survival was determined 48h later. Motor neuron death induced by trophic factor deprivation (NONE) was used as a control. Data are expressed as percentage of control GDNF (meanSD). *Significantly different from control GDNF ( .05); Significantly different from hSOD1 control ( .05). For all panels, experiments were performed in three independent cultures performed in duplicate and the average of each experiment is shown. Each experimental replica is shown for control groups",yes
PMC10463916,Figure_1,oa_package/e2/da/PMC10463916.tar.gz,"[' 1) [4, 5].', 'cSS on MRI and histopathology.', 'D Inset of iron stain indicated in C demonstrating iron deposits in the leptomeninges and superficial cortexcSS is one of the most important predictors of first-time and recurrent ICH in patients with CAA [7, 8].', ' 1.']","Fig. 1 cSS on MRI and histopathology. In vivo GRE MRI scan of a patient with a clinical diagnosis of probable CAA with multifocal cSS (arrowheads). Ex vivo T2-weighted turbo-spin echo (TSE) MRI of one hemisphere from the same case, * indicates region of cSS. Histopathological section from region outlined in B stained for iron. Inset of iron stain indicated in C demonstrating iron deposits in the leptomeninges and superficial cortex",yes
PMC9793114,Figure_2,oa_package/91/a7/PMC9793114.tar.gz,"['Flat low cuboidal cells expressed, in immunohistochemistry (IHC), calretinin and N-terminus WT1 (nuclear staining) ().', 'Histological examination of the lesion:(A) The cyst has a collagenous and moderately cellular wall (Scanning power).', ')Clinical and radiological assessments performed in the outpatient unit reveled no recurrence after a 6-month follow up.']","Fig. 2 ) The cyst has a collagenous and moderately cellular wall The cystic lining is composed of tall columnar cells ( ) and flat low cuboidal cells ( ). Immunohistochemistry showed that flat low cuboidal cells expressed calretinin and WT1 both being mesothelial markers. . (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)",yes
PMC8492311,Figure_6,oa_package/69/35/PMC8492311.tar.gz,"['Mining previously published gene expression data from human acute wounds at several time points (26), we found that FSCN1 mRNA expression is upregulated 3 and 7 days after wounding, concomitant with wound reepithelialization (A).', 'To better characterize the expression of FSCN1 in relation to GRHL3 in human acute wounds, we xenografted surgically discarded human skin isolated from healthy subjects onto the dorsal back of immunodeficient mice (B; n = 3).', 'Following recovery, a small wound (2 mm) was created on the xenograft, and wound tissue was collected 3 days after wounding, during the period of active reepithelialization (B).', 'Consistent with our mouse results, E-cadherin and FSCN1 expression was largely complementary in keratinocytes at the wound front; FSCN1 was upregulated in the wound front, with higher expression in basal keratinocytes where E-cadherin expression was almost absent (C).', 'GRHL3 was also upregulated in keratinocytes in the wound front, with positive correlation with FSCN1 expression (C).', 'Although FSCN1 was detected in some basal keratinocytes in diabetic wounds, it was downregulated in suprabasal keratinocytes in the wound front compared with controls (E and Supplemental A).', 'Consistent with this finding, we observed more E-cadherin expression at the cell surface in keratinocytes at the diabetic wound front, similar to the E-cadherin expression pattern in Grhl3-cKO wounds (, E and F).', '5 (Supplemental B) and in migrating human keratinocytes in vitro (Supplemental C).', 'We also show that the FSCN1/E-cadherin pathway is altered in diabetic wounds, although this appears to be independent of GRHL3 ().', 'The GRHL3/FSCN1/E-cadherin pathway is activated in acute human wounds and impaired in diabetic mouse wounds.']","Figure 6 The GRHL3/FSCN1/E-cadherin pathway is activated in acute human wounds and impaired in diabetic mouse wounds. ( ) mRNA expression (mean SEM) in acute human wounds at the indicated times after wounding. ( ) Schematic representation of the acute wound model using human skin xenografts ( = 3). ( ) Immunofluorescence analysis of FSCN1 (green), E-cadherin (red), and GRHL3 (magenta) in human wounds at day 3 after wounding. Dashed lines indicate the basis of the migrating epithelium in the wound front. Scale bar: 70 m. ( ) Schematic representation of the mouse diabetic wound model ( = 3). ( ) Immunofluorescence analysis of FSCN1 (green), E-cadherin (red), and DAPI (blue) in nondiabetic and diabetic mice at day 3 after wounding. Scale bar: 200 m. Dashed lines indicate the basis of the migrating epithelium in the wound front. White boxes indicate the area of magnified images (rightmost panel). Scale bar: 45 m. ( ) Integrated intensity of E-cadherin staining in nondiabetic and diabetic wound sections ( = 3). Statistical significance was determined using Students t test (* < 0.05).",yes
PMC7519950,Figure_1,oa_package/dc/98/PMC7519950.tar.gz,['Antero posterior chest X ray (CXR) showing a large right sided pleural effusion due to ventriculo pleural (VPL) shunt.'],Figure 1 Anteroposterior chest Xray (CXR) showing a large rightsided pleural effusion due to ventriculopleural (VPL) shunt.,yes
PMC4899798,Figure_1,oa_package/53/5d/PMC4899798.tar.gz,"[' Patoc from 24 hpf for 5 days (A).', 'As shown in B, lipl32 mRNA was detected in the zebrafish larvae that were incubated with L.', 'We also examined the flagellin gene flaB mRNA, which showed a similar expression pattern but persisted until 72 hpf (B and Supplementary S1)31.', 'Leptospira Shermani infection in zebrafish larvae.']","Figure 1 Shermani infection in zebrafish larvae. ( ) Kaplan-Meier survival curves of zebrafish larvae incubated with Shermani (n=109), Patoc (n=109) and controls (n=110). Log-rank test =0.4248. Standard error was calculated by the Greenwood method. ( ) RT-PCR analyses show the expression of and mRNAs in zebrafish larvae incubated with Shermani but not with Patoc or E3 buffer. Zebrafish was used as a loading control. Cropped gel images are shown and the gels were run under the same experimental conditions. Uncropped gels are shown in .",yes
PMC8476873,Figure_4,oa_package/16/35/PMC8476873.tar.gz,"['G516R mRNA-injected embryos contain more wnt4 compared to controls (A).', 'G516R mRNA injected (Supplementary ).', 'This elevated transcript level of wnt4 was further confirmed by whole-mount in situ hybridization (B).', 'Supplementary wnt4 upregulation shows a dose-dependent response to COG4p.']","FIGURE 4 Expression of human elevates the transcript in zebrafish embryos. Quantitative PCR analyses of selective non-canonical Wnt pathway ligands in zebrafish embryos injected with human or mRNA. At 6 hpf, expression is significantly upregulated in embryos injected with mRNA, but not in those injected with mRNA. Similar upregulation of transcripts is observed at 10 hpf as well. Bar graphs represent average gene expression relative to the housekeeping gene . The data are presented as mean SD. One-way ANOVA with Tukeys multiple comparison tests was used. < 0.0001; ns, not significant. Representative images of whole-mount hybridization from control and treated embryos. Whole-mount hybridization analyses demonstrate that expression is elevated in embryos injected with mRNA (a, at 6 hpf; b at 12 hpf), but there are no obvious changes in embryos injected with human mRNA (a and b). (b, b) Arrows point to the restricted expression domain in the hindbrain of zebrafish embryos at 12 hpf; (b) arrows point to expanded expression of in the hindbrain region. At 12 hpf, expression intensity is not significantly changed in embryos injected with mRNA (c, d), compared to either control siblings (c, d) or human mRNA-injected embryos (c, d). The dotted black color lines landmark the degree of angle between the head and tail, which is much more increased in embryos injected with mRNA, suggesting defects in extension movement during gastrulation. Arrows in c, c and d, d indicate that is restricted to the dorsal midline; however, its expression pattern is dispersed in mRNA-injected embryos, suggesting that the convergence movement is impaired (c, white dotted line and arrow; d, there is no clear midline expression). Scale bars: 200 m. Experiments were performed in triplicates with similar results.",yes
PMC11458631,Figure_5,oa_package/f8/de/PMC11458631.tar.gz,"[' 5).', '\nH E-stained microscopic images of appendix during 2nd trimester at 10x magnification with appearance of muscularis mucosa\nAppendix mucosa appeared to be thinner in the first trimester.']",Fig. 5 H&E-stained microscopic images of appendix during 2nd trimester at 10x magnification with appearance of muscularis mucosa,yes
PMC11099850,Figure_1,oa_package/39/38/PMC11099850.tar.gz,"['There was effusion at the level of the prevertebral-retropharyngeal area and the atlantoaxial joint ().', '(a) Cervical MRI showing hyperintense prevertebral effusion and (b) edema of longus colli muscle and tendon on\nsagittal STIR image, and (c) bilateral effusion adjacent to atlantoaxial joints on axial T2-weighted image.']","Figure 1 (a) Cervical MRI showing hyperintense prevertebral effusion and (b) edema of longus colli muscle and tendon onsagittal STIR image, and (c) bilateral effusion adjacent to atlantoaxial joints on axial T2-weighted image.MRI: Magnetic resonance imaging; STIR: Short-time inversion recovery.",yes
PMC10059735,Figure_1,oa_package/b9/69/PMC10059735.tar.gz,"['The interventional radiologist should aim for a parallel placement of the 19G electrodes (maximum angulation 10 ) for an interelectrode distance of about 20 mm (15 24 mm) and for a tumor-free margin of at least 5 mm [48,49], as shown in .', '23133889275CT scan shows correct placement of the 4 electrodes (19 G) at the periphery of the pancreatic head tumor (interelectrode distance 20 mm and angulation 10 ).']",Figure 1 CT scan shows correct placement of the 4 electrodes (19 G) at the periphery of the pancreatic head tumor (interelectrode distance < 20 mm and angulation < 10).,yes
PMC10345138,Figure_2,oa_package/a6/9c/PMC10345138.tar.gz,"['2B), while stratification of all cell layers appeared comparable to those of age-matched WT mice (', '2A).', '2C, D, 34.', '2D, p 0.', 'INL and total retinal thickness is significantly reduced in retinas of K42E vs.', 'To study changes in gross morphology, we performed morphological analysis of neural retina.', '2) and reduction in TRT consistent with inner retinal degeneration.']","Fig. 2 INL and total retinal thickness is significantly reduced in retinas of K42E vs. WT mice. Representative SD-OCT images from PN 18-mo old WT ( ) and K42E ( ) SD-OCT images are shown. White arrowheads note the thinning of the K42E retina INL. Averaged INL ( ) and total retinal ( ) thickness from PN 1- to 18-mo shows progressive reduction of INL thickness in K42E mice ( ) compared to WT mice ( ) and significantly lower total retinal thickness at PN 3-mo and 18-mo. IPL inner plexiform layer, INL inner nuclear layer, ONL outer nuclear layer. Scale bar: 90m, panels , . Statistical significance: * 0.05, *** 0.001. K42E PN 1-mo, =3, PN 3-mo, =3, PN 6-mo, =3, PN 12-mo, =6, PN 18-mo, =3; WT PN 1-mo, =4, PN 3-mo, =4, PN 6-mo, =5, PN 12-mo, =9, PN 18-mo, =4.",yes
PMC6061813,Figure_3,oa_package/8a/ea/PMC6061813.tar.gz,"[""L'IRM montre une masse en iso ou en hyposignal en s quence T1, en hyper signal en s quence T2 par rapport aux muscles avec un rehaussement mod r intense ()."", ""IRM c r brale, coupes axiale pond r e en T1 (A) et coronale pond r e en T1 apr s injection de Gadolinium; (B) processus tumoral orbitaire gauche infiltrant, en hypo signal en T1, se rehaussant de fa on h t rog ne apr s injection du Gadolinium: Rhabdomyosarcome orbitaireGliome du nerf optique: le gliome du nerf optique repr sente 4% des tumeurs orbitaires [4], l'incidence retrouv dans notre s rie tait de l'ordre de 5%, il affecte les enfants entre 5 10 ans; environ la moiti des patients atteints de gliome de la voie optique ont une neurofibromatose (NF1).""]","Figure 3 IRM crbrale, coupes axiale pondre en T1 (A) et coronale pondre en T1 aprs injection de Gadolinium; (B) processus tumoral orbitaire gauche infiltrant, en hypo signal en T1, se rehaussant de faon htrogne aprs injection du Gadolinium: Rhabdomyosarcome orbitaire",yes
PMC10724790,Figure_5,oa_package/57/7c/PMC10724790.tar.gz,"['Posterior sagittal ano-recto-vagino-urethroplasty (PSARVUP) is similarly applied to patients with the short common channel in cloacal malformation ().', '.']","Figure 5. Appearance of the perineum of cloacal malformation case. The localization where the rectum will be pulled-through is marked with silk sutures just before the surgery. The urethra, vagina, and rectum are anastomosed to the perineum one by one with PSARVUP.",yes
PMC5456338,Figure_3,oa_package/88/f2/PMC5456338.tar.gz,"['Tissue GAG was elevated in all tissues analysed (liver, kidney, spleen, heart, lung and brain) in MPS I untreated mice compared to age-matched normal controls (, p 0.', 'Treatment of MPS I mice with rhodamine B did not alter tissue GAG levels in liver, kidney, heart, spleen and brain (, p 0.', 'The exception to this was lung GAG, which was significantly decreased in MPS I mice treated with rhodamine B (F, p 0.', 'Tissue GAG levels.']","Figure 3 Tissue GAG levels. Normal (grey bars), MPS I untreated mice (black solid bars) and MPS I mice treated with rhodamine B (black hatched bars) were analysed post-mortem at 6 months to determine GAG levels. Aliquots of triton extracted tissues were cpc/citrate precipitated and uronic acid was determined. Uronic acid is expressed as g total uronic acid for brain ( ), spleen ( ), liver ( ), heart ( ), kidney ( ) and lung ( ). Data is expressed as average + SEM for each group. ** indicates significantly different from normal ( < 0.001, one-way ANOVA, Tukeys post-hoc test). indicates MPS I treated significantly different from MPS I untreated ( < 0.001, one-way ANOVA, Tukeys post-hoc test).",yes
PMC11530929,Figure_1,oa_package/59/c8/PMC11530929.tar.gz,['\nCureus\n2023\n15\ne51214\n38283468\n.'],Figure 1. Transabdominal ultrasound of the left ovary.,yes
PMC4880309,Figure_4,oa_package/ca/a5/PMC4880309.tar.gz,"['The overexpression of miR-143 in untreated NT2/D1 cells downregulates -dystrobrevin protein at both total (A, left panel) and nuclear (A, right panel) level, compared with untreated NT2/D1-miR-C and untransfected NT2/D1 (+) control cells, thus confirming that -dystrobrevin expression is affected by miR-143 targeting in live cells (A).', 'After RA treatment, we found that the cell proliferation rate of NT2/D1-miR-143 cells was significantly decreased compared with NT2/D1-miR-C control cells (B), while the differentiation program does not seem to be affected, as we did not detect any significant variation in the mRNA expression of MAP2 (C), or NF-L (D).', 'g004miR-143 overexpression affects proliferation of RA-treated NT2/D1 cells, decreases -dystrobrevin protein level and increases synapsin I gene expression.', 'g004""/>Interestingly, we found that the expression of synapsin I was upregulated at both the mRNA and protein levels in untreated (day 0) NT2/D1-miR-143 cells (E and 4F), when miR-143 is transiently overexpressed and -dystrobrevin expression downregulated (A).', 'These data are also in line with our results in miR-143-transfected NT2/D1 cells in normoxia, where the decrease of -dystrobrevin protein level (A) well correlates with the increase of synapsin I expression (E and 4F) during the short-term overexpression of miR-143.']",10.1371/journal.pone.0156325.g004,yes
PMC6158386,Figure_4,oa_package/b7/7c/PMC6158386.tar.gz,[],"FIGURE 4 Protein expression of BDNF, NGF, and NTF3 after chronic fluoxetine administration in the hippocampus and PFC of wild-type, Creb1 and Creb1 Crem/ mice. Chronic fluoxetine treatment (10 mg/kg, ip, 1x daily, 21 days) induced BDNF upregulation in the hippocampus of male and female mice and in the PFC of female wild-type animals; the effect was abolished in Creb1 mice . Fluoxetine increased NGF and NTF3 levels in the PFC of male Creb1 mutants. Western blot analyses of the effects of fluoxetine administration on the protein levels of BDNF, NGF, and NTF3 in the hippocampus (left panel) and the PFC (right panel) of wild-type, Creb1 , and Creb1 Crem/ mice. Representative blots of BDNF, NGF, NTF3 and GAPDH in saline (wells 13)- and fluoxetine (wells 46)-treated wild-type (wells 1, 4), Creb1 (wells 2, 5) and Creb1 Crem/ (wells 3, 6) mice. Data are presented as the mean SEM. < 0.05, < 0.01 vs. saline-injected wild-type mice of the same sex. W/t, wild-type; SAL, saline; FLX, fluoxetine.",yes
PMC4075836,Figure_1,oa_package/d0/3c/PMC4075836.tar.gz,"['A chest X-ray revealed right-sided pneumothorax ().', ': monthsNIV: noninvasive ventilation; incident: acquisition of pneumothoraxITGV: intrathoracic gas volumeRaw: airway resistanceBic: bicarbonatePCO2: carbon dioxide tensionPO2: oxygen tensionSO2: oxygen saturationLVR: lung volume recruitmentPCF: peak cough flowMIC: maximum insufflation capacity\n\nIn A, a chest X-ray, taken at admission, shows right-sided pneumothorax.', ', peripheral alveolar) lesion, a bleb, or an emphysematous bulla ().']","Chart 1 Recommendations and complications for air stacking/deep passive lung insufflation. PCF: peak cough flow; AS: air stacking; LI: lung insufflation; NMD: neuromuscular disease. aExclusion criteria in thestudies. bVC and MEP of at least 0.56 L and 11 cmH2O, respectively, are mandatory to elevate PCF > 3 L/s using AS. cAScumbersome or impossible; passive lung insufflation remains feasible, according to Bach et al.(8) dDefined as chronicabnormalities in chest X-ray and SpO2 < 95% or FEV1/FVC ratio two standard deviations less than normal.",yes
PMC7658328,Figure_7,oa_package/d4/84/PMC7658328.tar.gz,"['As seen in a, spautin-1 effectively down-regulated cellular expression of Beclin-1 and ATG-5, indicating the reduced autophagic level.', '8%, respectively (b).', 'Autophagy inhibition attenuates endothelial functionality.', 'MRCP revealed extrahepatic fusiform dilation of the common bile duct without intrahepatic duct dilation, suggesting type I choledochal cyst.']","Figure 2 MRCP revealed extrahepatic fusiform dilation of the common bile duct without intrahepatic duct dilation, suggesting type I choledochal cyst.",yes
PMC3720593,Figure_7,oa_package/97/8b/PMC3720593.tar.gz,"['Staining of ARPE-19 cells in the presence of the different constructs is shown in A.', 'Since it is difficult to appreciate the changes in fibronectin polymerization by the immunostaining data, we carried out the DOC assay (B) to quantitate the changes in levels of insoluble fibronectin.', 'B shows that Fn52RGDS was more effective in lowering the level of fibronectin polymerization (41%), as compared to Fn52 (79.', 'g007Effect of Fn52 and Fn52RGDS on fibronectin polymerization and MMP expression in ARPE-19 RPE cells.', 'g007""/>Examination of Levels of MMPSince changes in levels of fibronectin polymerization were clearly seen in the case of ARPE-19 cells, we investigated the levels of MMPs generated by these cells (C) in the absence (lane 1) or the presence of the two scFv antibodies (lanes 3 and 4 respectively).', 'C shows that expression of MMP2 is significantly reduced upon exposure to the scFv antibodies; the irrelevant scFv O27, did not lead to any change in level of MMP2 expression (lane 2; 93.', 'Both show minimal levels of fibronectin expression; however, it was found that ARPE-19 cells synthesize appreciable amounts of fibronectin upon stimulation with patient-derived vitreous ().']",10.1371/journal.pone.0069343.g007,yes
PMC5159181,Figure_2,oa_package/38/18/PMC5159181.tar.gz,['\n:Abdominal CT shows a 2.'],Figure 2: Abdominal CT shows a 2.2-cm arterial enhancing lesion (marked by red arrow) in tail of pancreas consistent with an insulinoma.,yes
PMC4890852,Figure_3,oa_package/ec/06/PMC4890852.tar.gz,"['To determine whether NMNAT2 s chaperone function is required for p-hTau clearance, we first tested NMNAT2 WT and various mutants in a HEK293-tau cell line stably expressing a doxycycline-inducible human tau40 (A and 3B) [To further demonstrate that NMNAT2 s chaperone activity is required for p-hTau clearance in vivo, we examined the impact of NMNAT2-WT, -ED, - cATP and GFP overexpression on p-hTau levels in the hippocampus of rTg4510 mice (C).', 'The impact of GFP or NMNAT2-WT, -ED, - cATP expression on hTau was evaluated 1-mo post injection with immunostaining (C).', 'ref079"" ref-type=""bibr"">79] was employed to evaluate the impact of NMNAT2 and its variants on hTau processing, while an HA-antibody identified exogenous NMNAT2 or its variants (C and 3D).', '68) significantly reduced the levels of conformationally altered hTau recognized by the MC-1 antibody (C and 3D).', 'To quantify the impact of NMNAT2 overexpression on hTau processing, western blot analysis was conducted with tissue homogenates prepared from EGFP and NMNAT2-WT, -ED, - cATP rAAV transduced hippocampi of rTg4510 mice one mo postinjection (E and 3F).', '59) or GFP significantly reduced p-hTau levels in rTg4510 hippocampi while total hTau levels remained unchanged (F).']",10.1371/journal.pbio.1002472.g003,yes
PMC3499034,Figure_4,oa_package/5e/97/PMC3499034.tar.gz,"['Cortical cortical coupling however, was intact ().', 'Measuring functional connectivity via simultaneous brain imaging and brain stimulation.']","Figure 4 Corticalstriatal connectivity deficits during a simple sensorimotor integration task in cocaine users. Reprinted from Drug and Alcohol Dependence. Hanlon CA, Wesley MJ, Stapleton JR, Laurienti PJ, Porrino LJ. The association between frontal-striatal connectivity and sensorimotor control in cocaine users. . 2011;115(3):240243. Copyright 2011, with permission from Elsevier. ( ) During a simple finger tapping task (which requires frontal and striatal coordination including the dopamine system) controls have a significant functional coupling between both corticalcortical regions and corticalsubcortical regions. ( ) In cocaine users however, corticalsubcortical coupling was impaired despite intact corticalcortical coupling.",yes
PMC4195840,Figure_10,oa_package/19/f3/PMC4195840.tar.gz,[],Fig. 10 Axial PET/CT image shows intense FDG uptake in the right root of the tongue and floor of the mouth ( ) in a patient with recent surgical resection of recurrent SCC of the left hemitongue. Corresponding axial non-contrast CT image confirms post-surgical architectural distortion without an obvious underlying mass lesion ( ) on the right. Corresponding axial T2W ( ) and contrast-enhanced T1W ( ) images reveal a linear band of low signal intensity scar tissue on the left ( ). No tumour is seen on the right ( ). Muscle architecture in the right floor of the mouth appears normal on T2 but there is some diffuse muscular enhancement on the corresponding T1W image. Compensatory hyperactivity and post-surgical inflammatory findings due to contralateral scarring were considered as the cause of this unusually high FDG uptake. Follow-up of 2 years confirmed absence of recurrence,yes
PMC10504688,Figure_4,oa_package/e0/9c/PMC10504688.tar.gz,"['In diabetic mice, cerebral parasite load showed a strong positive correlation with IL-17A ( A,1) and PD-1 (', 'In nondiabetic mice, IL-17A was moderately correlated with the parasite load ( A,2).', 'In both diabetic and nondiabetic mice, claudin-1 expression showed a significant inverse correlation with the parasite load ( C,1 2).', 'Correlation between the cerebral parasite load and intestinal biomarkers using the Spearman correlation coefficient.', 'Cerebral tissuesIL-17AIn the case of infected untreated mice, IL-17A showed increased expression in the subgroup (A,1 B,1).']",Fig. 4 Correlation between the cerebral parasite load and intestinal biomarkers using the Spearman correlation coefficient. Open symbols and lines signify the total infected populations either diabetic or nondiabetic. Note # refers to the -value.,yes
PMC10792151,Figure_6,oa_package/b8/88/PMC10792151.tar.gz,"[' 6a), cytarabine, gemcitabine, ifosfamide, and vincristine have been associated with PRES [2].', ' 6b), a monoclonal antibody inhibiting VEGFA (vascular endothelial growth factor 1), have also been reported to trigger PRES [10].', 'Examples of posterior reversible encephalopathic syndrome (PRES) related to chemotherapeutic agents.', 'Subsequent MRI post-bevacizumab cessation demonstrates significant improvement in signal abnormalities on axial T2 (arrows, b3) and FLAIR (arrows, b4) imagesThe different imaging patterns of PRES, as classified by Bartynski, are summarized in (']","Fig. 6 Examples of posterior reversible encephalopathic syndrome (PRES) related to chemotherapeutic agents. A 52-year-old female with stage IV gallbladder carcinoma underwent a cisplatin-based chemotherapy regimen. Following the third cycle, she experienced worsening headache and giddiness, leading to hospital admission after two episodes of generalized tonicclonic seizures. Axial FLAIR images (a1, a2) display hyperintensities in bilateral parietal, occipital, and posterior temporal lobes. The corresponding axial DWI image (a3) reveals increased signal and low apparent diffusion coefficient (ADC) (not shown), suggestive of PRES. A follow-up axial FLAIR image (a4) six weeks post-chemotherapy cessation shows near-complete resolution, with only residual signal in the right lingual gyrus (curved arrow). A 45-year-old female with triple-negative breast cancer (TNBC) underwent mastectomy and received a Bevacizumab-based chemotherapy regimen. Axial T2 (b1) and FLAIR (b2) MRI brain images exhibit symmetrical hyperintensities in bilateral centrum semiovale and corona radiata (arrows), indicating Bevacizumab-related PRES. Subsequent MRI post-bevacizumab cessation demonstrates significant improvement in signal abnormalities on axial T2 (arrows, b3) and FLAIR (arrows, b4) images",yes
PMC10387048,Figure_1,oa_package/e6/48/PMC10387048.tar.gz,"[' 1A, B).', ' 1C).', ' 1D).', ' 1E).', 'MrgprB2 is expressed on meningeal mast cells and contributes to mechanical hypersensitivity in a mouse model of migraine-like pain.', 'To further confirm the role of MrgprB2 expression in the meninges and evaluate this mast cell-specific receptor s role in mediating migraine pain we utilized the minimally invasive dural stimulation migraine model19.', ' 1F, G).']","Figure 1 MrgprB2 is expressed on meningeal mast cells and contributes to mechanical hypersensitivity in a mouse model of migraine-like pain. ( ) Confocal images from transgenic mouse meninges in which the tdTomato (tdT) fluorescent protein is under the MrgprB2 promoter (MrgprB2 Cre tdT). ( ) Avidin staining was used to identify mast cells (green). ( ) MrgprB2 Cre tdT mast cells (red). The percentage of tdT cells that also were avidin-positive was 96%. Over 200 cells were counted from n=3 mice. ( ) MrgprB2 Cre tdT mast cells (red) shown near meningeal afferents (stained with Neurofilament 200, green). The scale bar is 50m in ( ) and 100m in ( ). ( ) Representative flow cytometric profile of MrgprB2 Cre MRGPRXB2 tdT (tdT ) mast cells. Cells were harvested from the meninges ( ) or peritoneal cavity ( ) and mast cells were identified as live c-kit FcRI cells. An n=3 of mice per group for both meningeal and peritoneal extracts. ( ) A non-invasive dural stimulation model was used to apply Compound 48/80 (1mg/kg) to the WT and MrgprB2 (mut) mouse meninges. ( ) Mechanical facial hypersensitivity was measured in male mice prior to dural injection and then 1h, 2h, 4h, and 24h after dural stimulation with Compound 48/80; WT n=11, MrgprB2 n=10. ( ) Female mice were also tested for mechanical hypersensitivity at 1h, 2h, 4h, and 24h after dural stimulation with Compound 48/80; WT n=6, MrgprB2 n=6. Error bar: S.E.M. *p<0.05, ****p<0.001.",yes
PMC6863614,Figure_1,oa_package/94/87/PMC6863614.tar.gz,"['In cases of TILs level of less than 10%, a 1% or 5% criteria was applied ().', 'g001Assessment of tumor-infiltrating lymphocytes (TILs) level.']",10.1371/journal.pone.0224430.g001,yes
PMC6897346,Figure_7,oa_package/6a/a3/PMC6897346.tar.gz,"['Chest and upper abdomen contrast-enchanced computed tomography (CT) in the venous phase, sagittal view, showing a common celiacomesenteric trunk (arrow).']","Figure 7 Chest and upper abdomen contrast-enchanced computed tomography (CT) in the venous phase, sagittal view, showing a common celiacomesenteric trunk (arrow).",yes
PMC3698899,Figure_15,oa_package/8d/99/PMC3698899.tar.gz,[],"Figure 15(A-D) A middle-aged man presented with progressive neck swelling after attempted biopsy. (A) Axial, (B) Sagittal CT angiography images show two partially thrombosed pseudoaneurysm (Arrow) arising from the right carotid artery. (C, D) DSA image shows the pseudoaneurysms (arrow) with no opacification of the ICA and faint distal run-off of ECA branches (arrowhead). Contralateral carotid angiogram revealed good intracranial cross circulation. (E, F) The pseudo aneurysms were treated by coiling (dotted arrow) and occluding ECA as well as CCA. No neurological deficit seen postprocedure",yes
PMC5639757,Figure_3,oa_package/32/39/PMC5639757.tar.gz,"[' 3).', 'Thoracic cavity of a drowned shag showing swollen and intensely congested lungs.', 'The white frothy fluid adjacent to the spine (yellow arrow) was released from the lungs when they were excised\nVentral view of the organs at the base of the neck and cranial thorax of a drowned guillemot.']",Fig. 3 Thoracic cavity of a drowned shag showing swollen and intensely congested lungs. The lungs have been excised and moved cranially for examination. The right lung is shown intact (black arrow) but the left lung was bisected and the cranial half removed; the cut surface of the caudal half (small arrows) shows that the intense congestion extends evenly throughout the parenchyma. The white frothy fluid adjacent to the spine (yellow arrow) was released from the lungs when they were excised,yes
PMC10434303,Figure_1,oa_package/ad/8e/PMC10434303.tar.gz,[],"Fig. 1. BB-Cl-amidine inhibits STING-dependent signaling. ( ) Structure of BB-Cl-amidine. ( and ) ELISA analysis of TNF- and IFN- in conditioned medium from BMDMs pretreated with vehicle control (DMSO) or BB-Cl-amidine (1 M) for 1 h followed by treatment with the indicated ligands for 24 h. ( ) ELISA analysis of IFN- in conditioned medium from BMDMs pretreated with vehicle control (DMSO) or BB-Cl-amidine (1 M) for 1 h followed by infection with HSV1 (MOI 10) or Sendai virus 20 (20 Units) for 24 h. ( ) qPCR analysis of expression in BMDMs pretreated with the indicated concentrations of BB-Cl-amidine followed by treatment with diABZI-4 for 2 h. ( ) ELISA analysis of IFN from BMDMs pretreated with the indicated concentrations of BB-Cl-amidine followed by treatment with diABZI-4 (500 nM) for 24 h. ( ) Kinetic cell death analysis of primary BMDMs treated with the indicated concentrations of BB-Cl and stained with Sytox Orange and Hoechst. Cells were imaged using the Cytation5 microscope. One read per hour for 12 h. ( ) Immunoblot analysis of phosphorylated STING, IRF3, TBK1, STAT1, P65, and LC3 conversion in whole-cell lysates from BMDMs pretreated with the indicated concentrations of BB-Cl-amidine for 1 h followed by treatment with diABZI-4 for 1 h. ( ) qPCR analysis of in human primary monocytes pretreated with BB-Cl-amidine (1 M) followed by treatment with 500 nM diABZI-4 for 2 h. ( and ) Serum analysis of IFN- and TNF- in mice administered BB-Cl-amidine (10 mg/kg) for 1 h followed by diABZI (0.5 mg/kg) for 5 h. , , and representative data. , and pooled data from three independent experiments. and vehicle (n = 4), diABZI-4 (n = 6), diABZI-4 + BB-Cl-amidine (n = 5). * < 0.05; ** < 0.001. Error bars show mean SEM.",yes
PMC8007996,Figure_6,oa_package/9a/b8/PMC8007996.tar.gz,['The terminal ileum enters the peritoneal cavity through the neck of the sac to reach the caecum ().'],"FIGURE 6 Graphic illustration of a left paraduodenal hernia demonstrating jejunal loops prolapsing into the fossa of Landzert, lateral to ligament of Treitz and posterior to the inferior mesenteric vein and ascending branch of left colic artery.",yes
PMC7363634,Figure_7,oa_package/7d/65/PMC7363634.tar.gz,"['The ER staff obtained new imaging ( 7, 8), but the patient left against medical advice before the orthopaedics team could see him.', ' 7Radiograph of the abdomen status after left hip disarticulation demonstrating continued erosion of the left hemipelvis.']",Figure7 Radiograph of the abdomen status after left hip disarticulation demonstrating continued erosion of the left hemipelvis.,yes
PMC8354305,Figure_7,oa_package/6d/66/PMC8354305.tar.gz,"['7).', ', with permission from ElsevierLiver and GallbladderACE2, the SARS-CoV-2-binding receptor, is highly expressed in liver cells.']","Fig. 7 Houston case Two (HC2). (A and B) Myocardium is edematous and small blood vessels are congested. The CMC exhibit multifocal vacuolar degenerative changes. No inflammatory cellular infiltrates are present. Note the increased width of these CMC compared to those of HCO ( B). The patient's heart weighed 1070 g. (C) The epicardium exhibits a lymphocytic infiltrate adjacent to a vein. (D) Testis with thrombi in peritesticular veins. (A and B, one-micron sections, toluidine blue stain; C and D, hematoxylin and eosin stains). (Magnification bar: A and B, 20 m; C, 100 m; D 500 m). Reproduced/adapted from Buja et al., with permission from Elsevier",yes
PMC3527696,Figure_8,oa_package/f1/20/PMC3527696.tar.gz,"['Enzyme-linked immunosorbent assays for A 40 (A,B) and 42 (C,D) were performed on total brain extracts from mice exposed to blast injuries of 147 kPa and harvested at 24 h post-blast exposure.']",Figure 8 . Controls consisted of sham exposed mice. Values are presented the SEM. Asterisk indicates values that were significantly different from controls (unpaired -tests). Note the reduction in A 40 and 42.,yes
PMC1820591,Figure_1,oa_package/cf/95/PMC1820591.tar.gz,['A silicone breast implant in this patient caused marked limitation of echocardiographic acoustic window and image acquisition in the parasternal long axis view obscuring left ventricular cavity and septum.'],Figure 1 A silicone breast implant in this patient caused marked limitation of echocardiographic acoustic window and image acquisition in the parasternal long axis view obscuring left ventricular cavity and septum.,yes
PMC11569822,Figure_5,oa_package/b8/f9/PMC11569822.tar.gz,"['Histopathological examination of the resected mass revealed lipomatous hypertrophy of the interatrial septum with no signs of malignancy (A: H E staining, magnification: 400; B: H E staining, magnification: 200)AT: adipose tissue, MT: muscular tissue, H E: hematoxylin and eosinDiscussionCardiac lipomas may be symptomatic or asymptomatic and found incidentally during a diagnostic procedure for an unrelated ailment [12].']","Figure 5 Histopathological examination of the resected mass revealed lipomatous hypertrophy of the interatrial septum with no signs of malignancy (A: H&E staining, magnification: 400; B: H&E staining, magnification: 200) AT: adipose tissue, MT: muscular tissue, H&E: hematoxylin and eosin",yes
PMC5688437,Figure_5,oa_package/f3/5b/PMC5688437.tar.gz,"[' 5).', 'Percentage of cats that had heartworms or worm fragments at necropsy (Adult HW)\nRadiographsOn Day 240, cats in Groups B and C had radiographic scores for main pulmonary artery, arterioles, and bronchial interstitial changes which were not consistently different from each other (Figs.']","Fig. 5 Serology results in cats after infection. Treatment groups: Group A, selamectin monthly initiated on Day 28; Group B, oral ivermectin every 2weeks initiated on Day 70 PI; Group C, infected, untreated. Percentage of cats in each group that were positive at any time point for heartworm antibody (AB Pos Days 70240) or antigen (ANG Pos Days 70240). Percentage of cats that had heartworms or worm fragments at necropsy (Adult HW)",yes
PMC6918585,Figure_3,oa_package/37/c0/PMC6918585.tar.gz,"[' 3.', '', 'CM clustered mitochondria\nAutophagyWith regard to autophagy, we examined in individuals of REF and iNPH cohorts on average 12.']","Fig.3 The distance between mitochondria and endoplasmic reticulum is reduced resulting in increased number of MERCs in iNPH. The endoplasmic reticulum (ER) and normal mitochondria (NM) are shown in a REF individual, also demonstrating the MERCs. In an iNPH patient the MERCs is indicated. The MERCs distance was shorter in iNPH. At group level, the MERCs was 4.4-fold longer in REF than iNPH individuals (0.240.04m vs. 0.060.03m; P<0.001; multivariate analysis). The length of ER was similar in the REF versus iNPH cohorts (0.670.30m vs. 0.780.43m, P=0.65). Error bars are 95% CI. Magnification 11,500; Scale bar 1m. clustered mitochondria",yes
PMC6003690,Figure_2,oa_package/56/d4/PMC6003690.tar.gz,"['The spatial distribution of t-WM (in red, ) clearly mapped on well-formed parts of projection and commissural tracts in deep WM, e.', 'g002Spatial distribution of the average t-WM (red), f-WM (blue) and c-WM (green).', 't004"">Table 4 and ).']",10.1371/journal.pone.0196297.g002,yes
PMC4422191,Figure_3,oa_package/c9/fa/PMC4422191.tar.gz,"['Effect of noopept on 25 35-evoked disturbances of intracellular calcium level, ROS accumulation and mitochondrial function.']",Figure 3 Pre-treatment with noopept reduces the rate of intracellular calcium in PC12 cells exposed to A. Noopept diminishes - induced enhancement of reactive oxygen species generation. Noopept exposure ameliorates the mitochondrial membrane potential of PC12 cells after -caused stress. Results represent meansSEM. The values were obtained from three independent experiments with five technical replicates (A) and from five independent experiments with four technical replicates (B and C).,yes
PMC7254183,Figure_5,oa_package/e7/6e/PMC7254183.tar.gz,"['PT on CT in a 23-year-old male patient with aplastic anemia, and with a duration of 4 days after catheter drainage.']","Figure 5 PT on CT in a 23-year-old male patient with aplastic anemia, and with a duration of 4 days after catheter drainage. PTgas in right pleural cavity (black arrow). With an infectious lesion in the lower right lobe with the pleura involved (white arrow). CT=computed tomography, PM=pneumomediastinum, PT= pneumothorax.",yes
PMC9700114,Figure_1,oa_package/0e/d2/PMC9700114.tar.gz,"['Molecular and cellular analyses identified age- and injury-related changes in innate immune and autophagy pathways late after TBI ().', 'Relationship between microglial functions in normal aging and chronic TBI.']","Figure 1 Relationship between microglial functions in normal aging and chronic TBI. The diagram depicts various cellular and molecular changes in microglial function across the normal lifespan and chronically following traumatic brain injury (TBI). The resemblance in the functional phenotype of microglia from young chronic TBI (< 12 months old) and normal aged (> 18 months old) mice, and progressive worsening with increased age at the time of injury is consistent with the notion of injury-induced accelerated aging (created with Biorender.com).",yes
PMC11098145,Figure_1,oa_package/01/05/PMC11098145.tar.gz,"['Portraits of these trichoscopy findings can be observed in .', 'ijt_31_22-F1"">(a) AGA.']","Figure 1 (a) AGA. Hair diameter diversity is more than 20%, peripilar sign (blue arrowhead). (b) AGA. White dots (red arrowhead). (c) AGA. Empty follicles and vellus hair (green arrowhead). (d) AGA. Single-hair follicles (yellow arrowhead). AGA: Androgenetic alopecia",yes
PMC3065735,Figure_1,oa_package/63/df/PMC3065735.tar.gz,"['Examples of diagnostic role of FDG PET/CT in inflammation, infection and neoplasia are presented in s 1 4.', 'Acta Chirurgica Belgica2005105189921579021014KnockaertDCMortelmansLADe RooMCBobbaersHJClinical value of gallium-67 scintigraphy in evaluation of fever of unknown originClinical Infectious Diseases1994184601605803831615PetersAMNuclear medicine imaging in fever of unknown originQuarterly Journal of Nuclear Medicine199943161731023028216MellerJIvancevicVConradMGratzSMunzDLBeckerWClinical value of immunoscintigraphy in patients with fever of unknown originJournal of Nuclear Medicine199839712481253966940317MellerJAltenvoerdeGMunzelUFever of unknown origin: prospective comparison of [18F]FDG imaging with a double-head coincidence camera and gallium-67 citrate SPETEuropean Journal of Nuclear Medicine20002711161716251110581718KjaerALebechAMDiagnostic value of In-granulocyte scintigraphy in patients with fever of unknown originJournal of Nuclear Medicine20024321401441185047619PetersAMHenkinREImaging infection and inflammation with Tc-99m-HMPAO-labeled leukocytesNuclear Medicine20062nd editionMount Joy, Pa, USAMosby1678169520Bleeker-RoversCPvan der MeerJWMOyenWJGFever of unknown originSeminars in Nuclear Medicine200939281871918780121JaruskovaMBelohlavekORole of FDG-PET and PET/CT in the diagnosis of prolonged febrile statesEuropean Journal of Nuclear Medicine and Molecular Imaging20063389139181657230422MellerJSahlmannCOScheelAKF-FDG PET and PET/CT in fever of unknown originJournal of Nuclear Medicine200748135451720469723OyenWJGMansiLFDG-PET in infectious and inflammatory diseaseEuropean Journal of Nuclear Medicine and Molecular Imaging20033011156815701457909824LorenzenJBuchertRBohuslavizkiKHValue of FDG PET in patients with fever of unknown originNuclear Medicine Communications20012277797831145305125Bleeker-RoversCPDe KleijnEMHACorstensFHMVan Der MeerJWMOyenWJGClinical value of FDG PET in patients with fever of unknown origin and patients suspected of focal infection or inflammationEuropean Journal of Nuclear Medicine and Molecular Imaging200431129371455175226Bleeker-RoversCPVosFJMuddeAHA prospective multi-centre study of the value of FDG-PET as part of a structured diagnostic protocol in patients with fever of unknown originEuropean Journal of Nuclear Medicine and Molecular Imaging20073456947031717135727BuysschaertIVanderschuerenSBlockmansDMortelmansLKnockaertDContribution of fluoro-deoxyglucose positron emission tomography to the work-up of patients with fever of unknown originEuropean Journal of Internal Medicine20041531511561524571628FedericiLBlondetCImperialeAValue of F-FDG-PET/CT in patients with fever of unknown origin and unexplained prolonged inflammatory syndrome: a single centre analysis experienceInternational Journal of Clinical Practice201064155601847936429KeidarZGurman-BalbirAGaitiniDIsraelOFever of unknown origin: the role of F-FDG PET/CTJournal of Nuclear Medicine20084912198019851899704030BalinkHCollinsJBruynGGemmelFF-18 FDG PET/CT in the diagnosis of fever of unknown originClinical Nuclear Medicine200934128628682013981831KeiaPLKokaTYPadhyaAKNgaDCGohaAS[18F] FDG PET/CT in patients with fever of unknown origin: a local experienceNuclear Medicine Communications20103197887922063476932JasperND britzJFroschMLoefflerMWeckesserMFoellDDiagnostic value of [(18)F]-FDG PET/CT in children with fever of unknown origin or unexplained signs of inflammationEuropean Journal of Nuclear Medicine and Molecular Imaging20103711361451952623433KremMMPanLBlinderMA(18)F-FDG-PET-facilitated diagnosis of lymphoma presenting with fever of unknown origin and cold agglutinationLeukemia and Lymphoma20074836196221745460834BlockmansDKnockaerDMaesAClinical value of [(18)F]fluoro-deoxyglucose positron emission tomography for patients with fever of unknown originClinical Infectious Diseases20013221911961117090735KjaerALebechAMEigtvedAH jgaardLFever of unknown origin: prospective comparison of diagnostic value of 18F-FDG PET and 111 In-granulocyte scintigraphyEuropean Journal of Nuclear Medicine and Molecular Imaging20043156226261473040336Bleeker-RoversCPBredieSJHvan der MeerJWMCorstensFHMOyenWJGF-I8-fluorodeoxyglucose positron emmission tomography in diagnosis and follow-up of patients with different types of vasculitisNetherlands Journal of Medicine200361103233291470891037UmekitaKTakajoIMiyauchiS[18F]fluorodeoxyglucose positron emission tomography is a useful tool to diagnose the early stage of Takayasu s arteritis and to evaluate the activity of the diseaseModern Rheumatology20061642432471690637638FerdaJFerdov EZ hlavaJMatejovicMKreuzbergBFever of unknown origin: a value of F-FDG-PET/CT with integrated full diagnostic isotropic CT imagingEuropean Journal of Radiology201073351852519201122A 54-year-old woman with FUO underwent PET-CT for the diagnosis of underlying disease.']","Figure 1 A 54-year-old woman with FUO underwent PET-CT for the diagnosis of underlying disease. Transaxial slices of CT (a1, b1), fusion (a2, b2), and PET (a3, b3) and anterior MIP image (c) showed accumulation of FDG in the wall of thoracic aorta and the supra-aortal branches. Gigantocellular arteritis was confirmed subsequently by temporal arterial biopsy.",yes
PMC9013596,Figure_7,oa_package/59/38/PMC9013596.tar.gz,[],FIGURE 7 (Case 16): (A) The right ankle ulcer with drawing excision design. (B) The reconstruction with free anterolateral thigh (ALT) flap after 2days,yes
PMC10232246,Figure_7,oa_package/c5/e7/PMC10232246.tar.gz,"['For that, we developed two phosphomutants of G3BP1: a phosphomimetic S149D mutant and a non-phosphorylatable S149A mutant (Supplementary ).', 'For that, lentiviral particles encoding G3BP1 were injected in one hemisphere of the striatum of wild-type C57BL/6 mice, while the contralateral hemisphere was injected with PBS, as control (A).', 'At 4 weeks post-injection, the loss of the neuronal marker DARPP-32 (B) in the hemisphere injected with G3BP1 was significantly lower compared to the control hemisphere injected with PBS (G3BP1: 0.', '035) (B and C).', 'Astrocytes activation was analysed by detection of the GFAP marker and compared between the G3BP1-injected hemisphere and the PBS-injected control hemisphere (D).', 'No differences were found in the immunoreactivity of GFAP between hemispheres (E).', '\nOverexpression of lentiviral vectors encoding G3BP1 in the brain of wild-type mice did not produce neuronal marker loss or inflammation.', 'The fact that G3BP1 expression in wild-type animals did not originate gross alterations indicative of toxicity () suggests that viral delivery of G3BP1 may constitute a reasonable strategy to treat SCA2 and SCA3, worthy of further exploration.']","Figure 7 Mice at 812 weeks of age were stereotaxically injected into the striatum (bilaterally) either with PBS or with lentiviral particles encoding for human G3BP1 and euthanized for tissue collection 4 weeks after injection. ( ) Schematic representation of the injection site in the striatum. ( ) Immunohistochemistry images analysis of DARPP-32 depletion volume (dashed line; ; scale bar = 200 m) and G3BP1 ( ; scale bar = 50 m) labelling in brain sections from mice injected with PBS and in the contralateral hemisphere injected with lentiviral particles encoding for G3BP1. ( ) The total area of DARPP-32 depletion, in mice brain sections, was reduced on injection of lentiviral particles encoding for G3BP1 when compared to the injection with PBS ( = 4; * < 0.05; Student's test). ( ) We also labelled GFAP, a marker of astrogliosis, in brain sections from mice injected with PBS and in the contralateral hemisphere injected with lentiviral vectors encoding G3BP1. ( ) The quantification of GFAP immunoreactivity did not detect significant differences between both hemispheres. Scale bar = 200 m. Values are expressed as mean SEM.",yes
PMC9572898,Figure_2,oa_package/20/25/PMC9572898.tar.gz,"['Stroke is typically divided into ischemic stroke and hemorrhagic stroke ().', 'Difference between ischemic and hemorrhagic stroke.']",Figure 2 Difference between ischemic and hemorrhagic stroke.,yes
PMC11318702,Figure_5,oa_package/09/34/PMC11318702.tar.gz,['.'],Figure 5. Hematoxylin and eosin stain (400) showing sheets of bland cells with smooth nuclear borders and salt and pepper chromatin.,yes
PMC6415517,Figure_2,oa_package/94/5f/PMC6415517.tar.gz,"['\nSurgical planning of the incision.', '\nPlanejamento cir rgico da incis o.']",Fig. 2 Surgical planning of the incision.,yes
PMC8412112,Figure_6,oa_package/ea/12/PMC8412112.tar.gz,[],"FIGURE 6 Stellate astrocytes decrease from subcortical through to subventricular regions, while clasmatodendritic astrocytes increase",yes
PMC10530233,Figure_17,oa_package/e9/07/PMC10530233.tar.gz,[],"Figure 17 The 3D transesophageal echocardiography in demonstrating the deployed transcatheter aortic valve replacement with endocarditis on follow-up: ( , ) the reconstructed aortic valve defects from the 3D TEE data from different viewing angles.",yes
PMC6612190,Figure_5,oa_package/1c/77/PMC6612190.tar.gz,"[' 5a, b) [40].', '5a, c).', '5a, b).', '5c).', '5d-f).', '5g).', 'ACY-738 reduces cytoplasmic FUS levels in Tg FUS+/+ mice.', 'Data are presented as means SEMCollectively, these results indicate that ACY-738 partially prevented cytoplasmic mislocalization of FUS in the remaining motor neurons, which could positively affect their function.']","Fig. 5 ACY-738 reduces cytoplasmic FUS levels in Tg +/+ mice. Nuclear-cytoplasmic fractionation of spinal cord tissues of P60 non-Tg controls, vehicle- and ACY-738-treated Tg +/+ mice. The top 75 kilodalton panel is a lower contrast image of FUS, the second 75 kilodalton panel is a higher contrast image of FUS. Calnexin was used as a cytoplasmic marker and as reference for equal loading. Histone 4 was used as a nuclear marker and as reference for equal loading. Quantification of the ratio of murine FUS (mFUS) and human transgene FUS (hFUS) to calnexin (cytoplasm) or histone 4 (nucleus) and normalization to non-Tg controls. =4, One-way ANOVA with Tukeys multiple comparisons test. Nuclear-to-cytoplasmic ratio calculated from absolute total FUS levels in both fractions. =4, One-way ANOVA with Tukeys multiple comparisons test. Quantitative PCR analysis of mRNA expression levels of murine and human FUS in the spinal cord of P60 non-Tg controls, vehicle- and ACY-738-treated Tg +/+ mice, with Ap3b1 and Mon2 as reference genes and normalization to non-Tg controls. Fold change compared to non-Tg controls (FC). =6, Students t-test. Western blot of FUS in the spinal cord of P60 Tg +/+ mice and non-Tg controls. Calnexin levels were used as reference for equal loading. Quantification of the ratio of Hdac1, Hdac2 and Hdac3 to calnexin and normalization to non-Tg controls. Immunostaining of FUS and neurons (NeuN) in spinal cord of P60 vehicle- and ACY-738-treated Tg +/+ mice. Scale bar=35m. * <0.05. Data are presented as means SEM",yes
PMC6291399,Figure_1,oa_package/36/07/PMC6291399.tar.gz,"['Admission noncontrast head computed tomography (CT) exam demonstrated a 5 3 cm extra-axial mass with heterogeneous hypodensity located in the left cavernous sinus and the CPA cistern, significantly compressing the ipsilateral pons (\n\n).', ""Subsequent magnetic resonance imaging (MRI;\n\n) revealed a solid and cystic mass centered at the cavernous sinus and Meckel's cave with a bulky hemorrhagic extension to the CPA cistern."", '\nPreoperative axial (\nA\n) computed tomography (CT) of the head shows a complex mass within the left cavernous sinus and CPA cistern compressing the left pons.']","Fig. 1 Preoperative axial ( ) computed tomography (CT) of the head shows a complex mass within the left cavernous sinus and CPA cistern compressing the left pons. Magnetic resonance imaging (MRI) ( ) reveals a solid and cystic mass centered at the cavernous sinus and Meckel's cave with a bulky hemorrhagic extension to the CPA cistern. The main mass is isointense on T1-weighted images ( ) and heterogeneously isointense on T2-weighted images ( ) with multilocular small cysts containing hemorrhagic layering. The solid component of the lesion is avidly enhancing on postcontrast images ( ). Signal of diffusion-weighted sequence imaging is lower than the brain parenchyma ( ) with ADC value of 1,300643 ( 10 mm /s; ). CPA, cerebellopontine angle. ADC, apparent diffusion coefficient.",yes
PMC8382575,Figure_5,oa_package/5e/0e/PMC8382575.tar.gz,[],Figure5 B cell receptor (BCR) repertoire analysis. Box plot showing the top 10 high-frequency B cell clonotypes for each sample in the present study. Venn diagrams of the public B cell clonotypes within the Alzheimers disease (AD) and normal controls (NC). Averages of the InvSimpson index and ShannonWeiner index of each sample are used to compare the BCR diversity of the AD group and NC. Distribution of the CDR3 amino acid length of the BCR in the AD group and NC. and genes usage frequency stacked histogram showing the distribution of common and genes of the BCR in the AD group and NC respectively.,yes
PMC6615450,Figure_3,oa_package/65/e7/PMC6615450.tar.gz,['Epiduroscopic findings of inflammation.'],"Fig. 3 Epiduroscopic findings of inflammation. (A) Mild inflammation, (B) moderate inflammation with fibrosis, and (C) extensive inflammation with granulation and fibrosis.",yes
PMC7488891,Figure_5,oa_package/2e/da/PMC7488891.tar.gz,"['1177_2374289520951902-fig5""/>Question/Discussion Points, Part IVWhat Additional Clinically Relevant Information Could Histologic Sections of the Gallbladder Provide, Microscopically?', 'One possible consequences of prolonged inflammation and distension is gangrene (a form of tissue death because of ischemia or complete lack of blood flow and oxygen, ).']","Figure 5. Microscopic image of gangrenous gallbladder mucosa on intermediate power, H&E 20. Coagulative necrosis (gangrene) is indicated by the absence of nuclei (top portion of image) and presence of necroinflammatory debris (neutrophilic infiltrate with associated necrosis) (bottom portion of the image).",yes
PMC9477559,Figure_5,oa_package/02/b5/PMC9477559.tar.gz,['Contrast enhanced CT scan of the chest showing right lower lobe partly spiculated partly lobulated lung lesion in coronal section (A) transverse section (B).'],Figure 5 Contrast enhanced CT scan of the chest showing right lower lobe partly spiculated partly lobulated lung lesion in coronal section (A) & transverse section (B).,yes
PMC10569079,Figure_1,oa_package/f4/f4/PMC10569079.tar.gz,['Axial CT of the abdomen and pelvis with contrast: perigastric fat stranding (white arrows) and concentric gastric wall thickening (red arrow) are both present as a result of inflammation.'],Figure 1 Axial CT of the abdomen and pelvis with contrast: perigastric fat stranding (white arrows) and concentric gastric wall thickening (red arrow) are both present as a result of inflammation. CT: computed tomography,yes
PMC4439742,Figure_5,oa_package/0a/11/PMC4439742.tar.gz,"[""This becomes a major issue while shooting images of depigmented lesions such as vitiligo, nevus depigmentosus, or a hypopigmented patch in Hansen's disease []\nAdditional flexibility in terms of optical zoom, white balance, and exposure settings are not available in all smartphones."", '\nImages of a nevus depigmentosus patch in a child taken in auto flash mode.']",Figure 5 Images of a nevus depigmentosus patch in a child taken in auto flash mode. (a) Smartphone camera (BlackBerry Z10) (b) CANON EOS450D (c) NikonCoolPix 4800. The excessive whiteness of the lesion is evident in the image shot with the smartphone,yes
PMC5459331,Figure_6,oa_package/32/bb/PMC5459331.tar.gz,"['0 cells that were treated with YM155 under conditions similar to those employed in the transcriptome analysis (A 6D).', 'g006Validation of selected genes associated with p53 connection pathway was carried out using quantitative real-time polymerase chain reaction (qRT-PCR) and western immunoblotting.', 'The results, as shown in E, were confirmatory.']",10.1371/journal.pone.0178168.g006,yes
PMC9622766,Figure_6,oa_package/5c/3b/PMC9622766.tar.gz,"['As expected in normal skin, POSTN was produced in papillary dermis and around hair follicles (A).', 'Importantly, the modification of POSTN staining was much more pronounced in group 4, in which the global surface of positive area was 13-fold increased (D).']","FIGURE 6 Impact of the procedures on POSTN deposition. Representative images of POSTN area staining in control and LD skins (Scale bar: 100m). Distribution curves of POSTN ( axis correspond to the distance from the epidermis/dermis junction). The dotted line delineates the junction between papillary and reticular dermis (PD and RD, respectively). Comparison of the distribution curves of POSTN staining in the different groups. Quantification of total labeling area in the dermis determined by measuring the area under the distributions curve (Integrated Area) (Statistical test: two-way ANOVA with multiple comparison Turkeys test, < 0.05: *, < 0.01: **, < 0.001: ***, < 0.0001: ****).",yes
PMC6999640,Figure_5,oa_package/61/cd/PMC6999640.tar.gz,"['We identifed no alteration in dendritic complexity ((b); number of intersections per 10 m sholl: NS; two-way ANOVA), total dendritic length ((a,c); total dendritic length: WT 2807 326.', '87; t-test) nor branch number ((a,d); total dendritic branches: WT 55.', '1615302-F0005.', 'We identified decreased axon length in KO neurons ((e,f): total axonal length; WT 963.', '0055; t-test), and in axon branching ((e,g): number of axonal branches: WT 5.', 'Despite this, at this age in culture nascent dendritic processes of KO neurons were unaltered in their length ((e,h); nascent dendritic length: WT 412 37.', 'Distal generation of axonal swelling, comprised of ER accumulations in AP-4 deficiencyIn examining axonal extension in culture, we noted that KO axons exhibited dramatic distal swellings ((e,j,k); axonal swellings: WT 1.', 'As a result of this transient increase in axonal ATG9A, we found a significant reduction in the number of axonal swellings in KO axons ((l,m); swellings per neuron: UT 8.']",10.1080/15548627.2019.1615302-F0005,yes
PMC6843538,Figure_7,oa_package/b5/18/PMC6843538.tar.gz,"['Mortality in genotype IIR infected fish, first occurred on 19 dpe, peaked on 22 dpe (6 fish) (A), and reached 100% on 28 dpe.', '1, 7 and 29 dpe gill samples were quantified for parasite copy numbers (B, Table S2) revealing similar copy numbers on 1 and 7 dpe for both genotypes.', 'Detection of the parasite in blood (samples quantified on 1, 15 and 29 dpe) was less than 1 copy for both genotypes on 1 and 15 dpe (B).', 'Parasite quantities in the intestine (B, Table S2) followed a similar trend for both types: numbers increased over time, peaking on 22 dpe.', 'shasta Virulent GenotypesComparison of motility gene expression in the intestines of rainbow trout infected with virulent genotype IIR relative to low virulent genotype 0 revealed that four genes were upregulated: -actin, integrin- , talin and RhoA, with the highest fold changes observed for integrin- (up to 54-fold change) and -actin (up to 21-fold change) (C, Table S3).', 'The comparison of gene expression over time between genotypes revealed further differences and opposite trends (C, Table S4).', 'Ceratonova shasta genotypes 0 and IIR experimental infection dynamics in rainbow trout and motility genes expression.']","Figure 7 genotypes 0 and IIR experimental infection dynamics in rainbow trout and motility genes expression. ( ) Mortality curve (%) for type 0 and IIR over the course of the infection. ( ) Parasite SSU rDNA copy numbers (qPCR) in the gills, blood and intestine for genotype 0 and IIR at different days post exposure (dpe). ( ) Parasite motility genes expression in the intestine: Relative change (2 ) for each genotype over time and fold change (2 ) between genotypes (IIR:0).",yes
PMC8559346,Figure_2,oa_package/bd/b3/PMC8559346.tar.gz,"[' 2.', 'Full features extraction and classification work-flowData descriptionWhole Slide Images were collected between July 2011 and February 2015 by physicians from the Department of Emergency and Organ Transplantations of the Bari University Hospital (Italy).']",Fig. 2 Full features extraction and classification work-flow,yes
PMC10403219,Figure_4,oa_package/24/34/PMC10403219.tar.gz,[],"Fig.4. Nxf1 facilitates cytoplasmic translocation of TDP-43 monomers. ( ) Neuro2a cells transiently expressing TDP-43 -mCherry were treated with control siRNA or siRNAs targeting Xpo1 or Nxf1, followed by nucleocytoplasmic fractionation. Representative immunoblots showing the cytoplasmic (C) and nuclear (N) levels of TDP-43 (RFP), Xpo1, and Nxf1 in the Neuro2a cells expressing TDP-43 -mCherry. GAPDH and fibrillarin were used as cytoplasmic and nuclear markers, respectively. ( ) Relative cytoplasmic/nuclear ratios of TDP-43-mCherry in (A) plotted as means SEM. = 3, biologically independent experiments. ( ) Representative images of Neuro2a cells transiently expressing TDP-43 -mCherry treated with control siRNA or siRNAs targeting Xpo1 or Nxf1. Scale bar, 10 m. ( ) Box and whisker plots of the cytoplasmic/nuclear ratios of TDP-43-mCherry fluorescence quantified in 100 cells from three independent experiments in (C). The graph shows the quartiles (boxes), 50th percentiles (center lines), and ranges between the maximum and minimum values (whiskers). ( ) TDP-43-3FLAG WT or 6M mutant was transiently transfected in the Neuro2a cells. An anti-FLAG antibody was used for immunoprecipitation of the lysates. Bound endogenous Nxf1 was detected by immunoblotting with an anti-Nxf1 antibody. ( ) Relative Nxf1 levels bound to TDP-43-3FLAG was normalized to the immunoprecipitated FLAG levels (relative to TDP-43 ) in (E) plotted as means SEM. = 3, biologically independent experiments. ** < 0.01 and **** < 0.0001 [ANOVA with Tukeys test in (B) and (D) and unpaired two-sided test in (F)].",yes
PMC5480280,Figure_3,oa_package/f3/df/PMC5480280.tar.gz,"['3"">ICV-Delivered BMN 250 Results in Complete Clearance of Pathological Heparan Sulfate and Normalization of Lysosomal Storage PathologyAt 16 weeks of age, Naglu / mouse brains (untreated or vehicle-treated) showed highly elevated steady-state levels of total HS and disease-specific HS-chain non-reducing ends (NREs) with the trisaccharide A0I2S028 (N-acetyl glucosamine 2-sulfated iduronic acid N-sulfated glucosamine) compared with normal levels in Naglu+/ and Naglu+/+ controls (untreated or vehicle-treated) ( 3A).', '4% (mean SEM) in total HS and NREs, respectively, relative to the levels of vehicle-treated Naglu / mice 24 hr after the last infusion ( 3A).', 'This reduction persisted for at least 28 days, during which total HS levels were only 2-fold higher than those of unaffected controls, and NRE levels were no longer detectable ( 3A).', ' 3Reduction of Lysosomal Storage and Elevated -hexosaminidase Activity by BMN 250 at 1 and 28 Days following Four 100 g ICV Doses over 2 Weeks(A) Steady-state levels for total HS and disease-specific NREs in brain homogenate, shown as picomoles per milligram of tissue wet weight (mean SEM).', '6-fold) those in Naglu+/ and Naglu+/+ control mouse brains ( 3B).']","Figure3 Reduction of Lysosomal Storage and Elevated -hexosaminidase Activity by BMN 250 at 1 and 28 Days following Four 100g ICV Doses over 2 Weeks (A) Steady-state levels for total HS and disease-specific NREs in brain homogenate, shown as picomoles per milligram of tissue wet weight (mean SEM). (B) -Hexosaminidase enzyme activity (mean SEM) measured in brain homogenate. VEH, vehicle. n= 6 per group, except n= 2 for naive mice. **p< 0.01, ***p<0.001, ****p< 0.0001; ns, not significant.",yes
PMC11634980,Figure_2,oa_package/79/c1/PMC11634980.tar.gz,"['The obtuse angle formed by the intersection of these lines was recorded ().', 'Anteroposterior (AP) shoulder X-ray demonstrating the method for measuring the acromioclavicular (AC) angle.', 'Parameters Measurement Based on CTPatients who underwent cervical CT scans (Brilliance iCT, Philips, Netherlands)\nin Daping Hospital from July 1, 2020, to July 15, 2020, were included in the\nprimary scope of the study, and CT image data of 61 patients was acquired using\n1.', 'The following parameters were measured on the PACS system (Huahai Medical\ninformation corp, Xian, China) by 2 spine surgeons:Pedicle axis length (PAL): the distance from the EP to the anterior border of the\nvertebral body via the AA (PAL) or nAA (nPAL) (A).', 'Pedicle transverse angle (PTA): the angle between the midline of the vertebral\nbody and the AA (PTA) or nAA (nPTA) (B).', 'The purpose of\nthese 2 parameters were to evaluate the proper pedicle screw depth in the\nsagittal plane and width in the coronal plane (C, D).', 'Transverse angle (T-angle) and sagittal angle (S-angle): the angle between the\nnAA and the line connecting the EP and ipsilateral conjunction of the lamina and\nspinal process or the tangent line of the posterior surface of the lateral mass\nand inferior one (E,\nF).', 'ResultsParameters of nAA-CPS and AA-CPS Measured on CTThe parameters are summarized in Table 1, and Supplementary Table 1.']","Figure 2. Illumination of the parameters measured on a CT scan. A, The distancesfrom the EP to the anterior border of the vertebral body via the AA(PAL) or nAA (nPAL): A point is the EP of the nAA and M point is themidpoint of the pedicle. A, B are the distance of the nAA (nPAL); C, Dare the traditional CPS length via the AA (PAL). B, The angle betweenthe midline of the vertebral body and the AA (PTA) or nAA (nPTA): theorange angle (PTA) is the angle between the midline of the vertebralbody and pedicle axis projection and the yellow angle (nPTA) as theangle between the midline of the vertebral body and the nAA pedicle axisprojection. C, Sagittal pedicle screw depth ratio (S-DO): the ratio ofthe pedicle screw length within the vertebral body to the sagittalvertebral length. D, Transverse pedicle screw depth ratio (T-DO): theratio of the projected width of the screw within the vertebral body tohalf of the vertebral body width in the coronal view. E, T-angle: theangle between the nAA and the line connecting the EP and the conjunctionof the ipsilateral lamina and spinal process in the transverse plane ofthe C5 vertebra. F, S-angle: the angle between the nAA and tangent lineof the posterior surface of the lateral mass and the inferior one in theparamedian sagittal plane of the C5 vertebra.",yes
PMC9495364,Figure_7,oa_package/6a/79/PMC9495364.tar.gz,"['Qualitative results for the the object detection pipeline involving semantic segmentation and Grad-CAM on the images of the independent external validation set V4 are depicted in .', 'It can be seen from the images of , taken from the V4 dataset, that precision is very high.', 'Examples of the NDG-CAM method on the data from the Pathology Department of IRCCS Istituto Tumori Giovanni Paolo II.']","Figure 7 Examples of the NDG-CAM method on the data from the Pathology Department of IRCCS Istituto Tumori Giovanni Paolo II. Results are shown for the best architecture (DeepLab v3+ with ResNet18 backbone). ( ) Original images. ( ) Semantic segmentation. ( ) Instance segmentation after detection of centroids of the nuclei, with each color denoting a different nuclear instance.",yes
PMC4229875,Figure_1,oa_package/d2/14/PMC4229875.tar.gz,"['Examples of such lesions are illustrated in \n1 which shows H E-stained sections from a rat that was exposed to single 74.', 'The lesion in \n1 follows the course of a branch of the corticoamygdaloid artery and extends through the agranular insular cortex, external capsule and basolateral amygdala.', 'The lesion produced a misalignment of the superficial cortical layers of the agranular insular cortex ( \n1A, arrows).', 'A cavity deep to the superficial lesion contains tissue that was likely displaced by the blast ( \n1B, arrows).', 'Interestingly, despite the fact that this animal had received its last blast exposure 10 months previously, a focal hemorrhage extending into the central amygdaloid nucleus was present ( \n1D and E, black arrow).', 'The presence of polymorphonuclear leukocytes within this hemorrhage (inset \n1E) indicates that it was of recent origin.', '\nBlast-induced shear-related injuries in brain.', '\nBlast-induced intraventricular and intracerebral hemorrhage 10 months after blast exposure.', 'In contrast, in all the blast-exposed rats many microvessels showed varying degrees of pathology (s \n10,\n11,\n12,\n13,\n14,\n15,\n16 and\n17).', '1\nMicrovascular strictures in the blast-exposed brain.', '2\nBlast-induced degenerative changes in cerebral microvessels.', '3\nAdvanced degenerative changes in blast-exposed microvessels.', '4\nBlast-exposed microvessel with degenerative changes.', '5\nMicrovascular occlusion and degeneration.', 'More severely affected vessels frequently exhibited abnormal strictures where the vascular lumen was narrowed and amorphous material was often present in the lumen at the site of the stricture ( \n11).', ' \n12 shows degenerative changes in microvessel structure.', 'A dysmorphic endothelial cell nucleus is seen in the lumen of the vessel in \n12A.', 'In \n12D degenerative changes are seen within the nucleus of a perivascular cell.', 's 13 and\n14 show more advanced degenerative changes that extend to perivascular cells.', 'In \n13 the blast-exposed microvessel lumens (panels A-C) are irregular with apparent degenerative changes in perivascular cells, which are dysmorphic and display an amorphous chromatin structure.', 'An irregular bulge from the vessel wall is visible in the lumen of one of the microvessels in \n13C.', 'In the microvessel illustrated in \n14, an endothelial cell nucleus has been displaced into the vascular lumen while at the opposite end of this microvessel the smooth muscle (SM) layer is disrupted.', ' \n15 shows additional examples of vascular degeneration associated with luminal accumulation of heterogeneous amorphous material ( \n15A,B) and extensive degenerative changes in the remnant of a microvessel ( \n15C,D).', 'In the microvessel shown in s \n15C,D there is almost complete luminal occlusion where what appears to be a minor luminal opening is the only architectural feature suggestive of a vascular structure while the rest of the vascular and perivascular cell architecture is unrecognizable.', 'Again, despite the extensive degenerative changes in the microvessels in s \n13,\n14 and\n15, the surrounding neuropil in all panels appears normal.', '6\nChronic microvascular pathology following blast exposure.', '7\nChronic pathology in a penetrating cortical vessel following blast exposure.', 'Ultrastructural chronic microvascular pathology following blast exposureTo determine whether chronic microvascular pathology could be found following blast exposure, we examined sections from the frontal cortex of a rat that received three 74.', 'One such example is shown in \n16 in which amorphous material in the lumen of a microvessel creates a near complete occlusion.', ' \n17 shows an example of a penetrating cortical vessel with degenerative changes in the tunica media and adventitia.']","Figure 1 H&E stained sections from a rat sacrificed 10 months after receiving 3 74.5 kPa blast exposures. Panels , and are from serial sections taken 500 m apart. Panel shows a disruption of the normal continuity of the superficial cortical layers of the agranular insular cortex (arrows). The basolateral (BLA) and central amygdaloid (CA) nuclei are indicated. In panel , a lesion contains tissue (arrows) that appears to have been avulsed and displaced. Panels and show two lesions. One appears to follow the course of a branch of the corticoamydaloid artery (white arrow). There is also an area of hemorrhage (black arrow), which is shown at higher power in panel . The inset in panel shows a higher power image of the hemorrhage itself. A polymorphonuclear leukocyte is indicated by an arrowhead. Sections from control animals are shown in panels and . Scale bar: 200 m , and ; 100 m and ; 50 m . Scale bar for inset in panel : 10 m.",yes
PMC9403278,Figure_1,oa_package/39/3d/PMC9403278.tar.gz,"['Findings were suggestive of neoplasia (A-B).', '(A-B): Preoperative MRI imaging showing the Synovial Spindle Cell Sarcoma in cross-sectional and coronal views, respectively.', 'To clearly visualize the situation, a Computed Tomography (CT) scan was performed to exclude metastasis.']","Fig. 1 (A-B): Preoperative MRI imaging showing the Synovial Spindle Cell Sarcoma in cross-sectional and coronal views, respectively.",yes
PMC7526468,Figure_4,oa_package/78/94/PMC7526468.tar.gz,['Post-operative radiograph for Case 2.'],Figure 4 Post-operative radiograph for Case 2.,yes
PMC4912349,Figure_3,oa_package/5f/9b/PMC4912349.tar.gz,"['4 cm was seen on CT (A, 3B).', '.']","Figure 3. ( ) Coronal reformatted CT images obtained approximately 3 and 6 years, respectively, after initial diagnosis of endometrial cancer, demonstrates a new right adrenal nodule (orange arrow).",yes
PMC5995925,Figure_4,oa_package/d8/fe/PMC5995925.tar.gz,"[' 4A and B).', ' 4A and C).', '(A) Immunofluorescence analysis for the expression of ASC protein and Caspase-1 showing increased expression in RCTI group on comparison with control.', 'Immunofluorescence analysis for the expression of (A) NLRP3 and (C) IL-1 showing increased expression in RCTI group on comparison with control.']","Figure 4 ( ) Immunofluorescence analysis for the expression of ASC protein and Caspase-1 showing increased expression in RCTI group on comparison with control. Images in the top row are histological sections of control group, and images in the bottom rows are histological sections of RCTI tendons harvested at 35 days (Group-1), 1012 days (Group-2) and 2224 days (Group-3). Images in the left column show nuclear staining with DAPI; the images in the middle column show expression of ASC protein and Caspase-1 while the images in the right column show overlay of ASC protein and Caspase-1 staining with DAPI. Images were acquired at 20x magnification using CCD camera attached to the Olympus microscope. The image shows quantification of the expression of ASC protein ( ) and Caspase-1 (C). The intensity of gene expression as observed through immunofluorescence was acquired and the mean fluorescence intensity (MFI) was quantified from each contralateral control and RCTI specimen. The graphs represent MFI mean values with standard error. ( < < < ).",yes
PMC7685864,Figure_4,oa_package/e3/51/PMC7685864.tar.gz,"['Furthermore, there was no difference in SOD and GSH levels between the AA-pretreatment MPP+ group and MPP+ group following NF- B inhibition (s 4(e) and 4(f)).', '003""/>Inhibition of NF- B signaling blocks the antioxidative effects of ascorbic acid and perpetuates cytotoxicity in MPP+-treated astrocytes in vitro.']","Figure 4 Inhibition of NF- B signaling blocks the antioxidative effects of ascorbic acid and perpetuates cytotoxicity in MPP -treated astrocytes . Primary astrocytes were copretreated with 1mM of AA and 0.5mM of QNZ (EVP4593) for 24h and/or 1mM of MPP for another 24h. (a) The NF- B-inhibition efficiency was detected by measuring the phosphorylation of p65 by Western blotting ( = 3). (b) Intracellular ROS was measured using the carboxy-H2DCFDA method (scale bar, 100 m) ( = 3). (c) The expression of iNOS was detected by Western blotting ( = 3). (d) The expression of SOD1 was detected by Western blotting ( = 3). (e) The concentration of SOD in the supernatant from primary astrocytes was detected by ELISAs ( = 3). (f) The concentration of GSH in the supernatant from primary astrocytes was detected by ELISAs ( = 3). Data were obtained from three independent experiments. One-way ANOVAs followed by LSD pairwise comparisons were performed. was considered significant compared to control ( < 0.05, < 0.01, and < 0.001). was considered significant compared to MPTP or MPP ( < 0.05, < 0.01, and < 0.001).",yes
PMC8423340,Figure_2,oa_package/5c/3c/PMC8423340.tar.gz,['Histopathological studyImage A (hematoxylin and eosin) shows a histological slide of the right paratracheal lymph node.'],"Figure 2 Histopathological study Image A (hematoxylin and eosin) shows a histological slide of the right paratracheal lymph node. This image illustrates effaced architecture with a nodular pattern. There are large nodules composed mostly of small lymphocytes with occasional large atypical lymphocytes. The atypical lymphocytes show bi- to multilobed nuclei and distinct nucleoli. Occasional small granulomas are noted in the background. No necrosis is noted. Images B, C and D show positive staining for CD20, OCT2 and CD3, respectively.",yes
PMC7744641,Figure_4,oa_package/3d/e3/PMC7744641.tar.gz,"[""A diagnosis of Giant Meckel's diverticulum with intermittent subacute small bowel obstruction was confirmed.""]","Fig. 4 Coronal reformat image of oral contrast phase showing outline of lesion (denoted by red arrows) and its connection with ileal loops in pelvis (green arrow demonstrates ileal loop). Also, note the wide neck of communication (denoted by black arrows).",yes
PMC4633078,Figure_4,oa_package/6e/25/PMC4633078.tar.gz,"['The cells of this layer are nucleated and\ncalled transitional cells(18)\n(A).', 'The other skin layers are similar to the non glabrous skin (B).', 'Glabrous skin.']","Figure 4 Glabrous skin. Histology. Thicker corneous layer and presenceof stratum lucidum (asterisk). HFUS, cross-sectional view.Hyperechoic epidermis with bilaminar appearance (arrow).",yes
PMC3022224,Figure_5,oa_package/fa/0c/PMC3022224.tar.gz,"['The digits (toes) are then removed from the demineralized hind paws by cutting across the metatarsals (the arch of the foot ) midway between the tip of the toes and the hock, after which the paw is divided longitudinally into approximately equal halves by cutting just lateral to the tibia and between the 2nd and the 3rd digits ().', '004""/>Tissue trimming procedure for reliable and reproducible production of high-quality hind paw sections for histopathological analysis of immune-mediated arthritis in rodents.']","Figure 5 Tissue trimming procedure for reliable and reproducible production of high-quality hind paw sections for histopathological analysis of immune-mediated arthritis in rodents. A skeletal schematic diagram (a) shows the location of the cuts required to isolate the tibiotarsal region. As viewed from the lateral side (a (left panel)), the hind paw is separated from the distal limb just above the tibiotarsal (hock (or ankle)) region at the fur line, and the digits (toes) are removed. From the top (a (right panel)), the hind paw is divided longitudinally using a cut placed between metatarsals II and III, which will fall just to the outside of the talus (the uppermost bone to the left of the orange line) and tibia (the distal leg bone (not shown)) that articulates with the talus. The distal tibia ((b), bracketed by forceps) is identified at the proximal cut margin of isolated hind paws as an oval white bone with a yellow/brown core of bone marrow. The longitudinal dividing cut (d) is made from the dorsal side by engaging the blade at the proximal and distal margins of the sample and then cutting straight down; the dashed black oval indicates the position of the tibia behind the razor blade, indicating that the blade is located just to the side of this bone. The microscopic structure of hind paw sections taken through this region from a nonarthritic rat (c) will include the joint spaces most susceptible to induced models of immune-mediated arthritis (asterisks (*)) as well as the most affected tarsal bones (navicular (N) and talus (Ta)), the distal tibia (Ti), and sometimes the calcaneus (C (or heel)). Histologic stain: hematoxylin and eosin (H&E).",yes
PMC7170022,Figure_2,oa_package/4e/61/PMC7170022.tar.gz,['Resected specimen showing central pancreas with the mass (white arrow) and the spleen (black arrow)Case 2A 31-year-old female presented with the symptom of lower abdominal pain for the past eight months.'],Figure 2 Resected specimen showing central pancreas with the mass (white arrow) and the spleen (black arrow),yes
PMC9294466,Figure_2,oa_package/77/22/PMC9294466.tar.gz,['Preoperative CT scan: (A) A 1.'],"Figure 2 Preoperative CT scan: ( ) A 1.7-cm, regional conglomeration of dense, soft tissue is evident. ( ) Lesion in (A) is surrounded by benign-looking adipose tissue.",yes
PMC9652393,Figure_7,oa_package/ca/9a/PMC9652393.tar.gz,"[' 7a).', 'M-DNM2 forms larger oligomer structures, and it is functionally different to Ub-DNM2.', 'Increasing NaCl concentrations triggers dynamin depolymerization45.', ' 7b, Supplementary ', ' 7c, Supplementary ', ' 7d, Supplementary ', ' 7e), even at high ionic strengths (Supplementary ', ' 7f).', ' 7g).', ' 7h, i, Supplementary ', ' 7).', ' 7).', ' 7).']","Fig. 7 M-DNM2 forms larger oligomer structures, and it is functionally different to Ub-DNM2. Image of the elution of M-DNM2 and Ub-DNM2 following gel filtration chromatography. Column S200 10/300 was used, and salt concentration was 1.5M NaCl. Absorbance (Abs) at 280nm is represented as arbitrary units (arb. units). Stain-free gel images for M-DNM2 and Ub-DNM2 fractioned in either supernatant (S) or in the pellet (P) after incubation at several salt concentrations. Right, proportion of DNM2 in S and P was calculated. Stain-free gel images for M-DNM2 and Ub-DNM2 fractioned in either supernatant or in the pellet after incubation with GMP-PCP (PCP) or ( ) after incubation at low salt concentration followed by depolymerization with GTP (2mM GTP+4mM MgCl ) ( =3 independent experiments, ** =0.0091, *** =0.0002, **** <0.0001 by 2-way ANOVA with Sidaks post hoc test). Electron microscopy representative image of Ub-DNM2 and M-DNM2 at physiological salt concentration with or without PCP incubation. Scale bar=100nm. It was repeated in 3 independent experiments (with different protein productions). GTPase activity measured in a phosphate release assay using malachite green dye with a reaction time of 90min at 37C, expressed as absorbance at 650nm. Measure for both DNM2 isoforms at different salt concentrations without lipids or ( ) with 2-Diacyl-sn-glycero-3-phospho-L-serine (PS) lipid (110mM (*** =0.005), 220mM (*** =0.0023), 440mM (*** =0.0006), 880mM (*** =0.0024) by two-tailed unpaired test at each salt condition). Time course of basal GTPase activity at 37.5mM NaCl during a maximum time of 180min. Zoom of the first 30min is shown below (two-tailed unpaired test at each time point). % of DNM2 depolymerization at 37.5mM NaCl followed by addition of GTP (0.5mM GTP+2mM MgCl ) and incubation at different time points. The difference of DNM2 in the pellet between control condition (-GTP) and condition incubated with GTP is represented. , Result reproduced in independent experiments shown in Supplementary Fig. and , respectively. , , n in the figure indicates the number of independent experiments using protein from 2 different productions. Data are represented as mean valuesSEM. Source data are provided as a Source Data file.",yes
PMC8920343,Figure_5,oa_package/71/7d/PMC8920343.tar.gz,"['Several metabolic intermediates in the upstream glycolytic pathway, including glucose-6-phosphate and fructose-6-phosphate, and products of the auxiliary pathways, including ribitol and glycerol-3-phosphate, were lower in Ad-YAP transduced CMs than in Ad-LacZ transduced CMs (, A C).', 'In contrast, metabolic intermediates in the downstream glycolytic pathway, including PEP and 3-phosphoglycerate (3-PG), malate, cystathionine (a serine biosynthetic pathway product), l-alanine, and amino acids synthesized from l-aspartate, including l-lysine, l-methionine, l-threonine, and l-isoleucine, were significantly elevated in Ad-YAP transduced CMs compared with levels in Ad-LacZ transduced CMs (, A C).', 'Phosphorylation of PKM2 at Tyr105, which reduces PKM2 activity and induces cell growth, was significantly induced by YAP in CMs (, D and E).', 'Glucose metabolism is regulated by YAP in CMs.']","Figure 5 Glucose metabolism is regulated by YAP in CMs. ( and ) Metabolomics analysis of glucose metabolism was performed in NRVMs transduced with Ad-LacZ or Ad-FLAG-YAP for 6 days in serum-free DMEM/F12 medium. ( ) Heatmap represents the expression profile of intermediates of glucose metabolism. ( and ) Summary of the metabolomics analysis of glucose metabolism. Black and gray represent detectable and undetectable, respectively ( ). Box plots show a summary of intermediates of glucose metabolism ( ). 6 dishes from 3 independent experiments. See also . ( and ) YAP increased Tyr105 phosphorylated Pkm2 in NRVMs transduced with Ad-LacZ or Ad-FLAG-YAP. Representative immunoblots ( ) and a summary of quantification ( ) are shown. -Tubulin and PKM2 blots, serving as loading controls, were run in parallel and contemporaneously with other blots ( ). 5 dishes from 5 independent experiments. * 0.05, by 2-tailed, unpaired Students test ( and ). Data represent the mean SEM.",yes
PMC9305576,Figure_1,oa_package/8b/cb/PMC9305576.tar.gz,['\nAbdominal computed tomography results.'],Figure 1 A: Plain scan showed a transverse colonic mass; B: Space-occupying lesion showed obvious enhancement.,yes
PMC11558687,Figure_2,oa_package/6d/c9/PMC11558687.tar.gz,"['Because of the larger and more irregular appearance of the pseudoaneurysm as well as worsening parent vessel stenosis (), we decided to treat it before hospital discharge 3 days after the thrombectomy.', 'FIG.']",FIG. 2. Right ICA angiograms demonstrating a pseudoaneurysm in the midcervical segment during the patients first ( ) and second ( ) mechanical thrombectomies. Repeat right ICA angiograms 3 days after the second thrombectomy in posteroanterior ( ) and lateral ( ) projections show increased size and irregularity of the pseudoaneurysm. The PK Papyrus was successfully deployed with immediate occlusion of the pseudoaneurysm ( ).,yes
PMC8553378,Figure_5,oa_package/f5/5f/PMC8553378.tar.gz,['Colonoscopy image.'],Figure 5 Colonoscopy image.,yes
PMC9317097,Figure_6,oa_package/3a/fe/PMC9317097.tar.gz,"['An example of the application of the compared methods is shown in .', 'Stain normalization of source patch (a) to target patch (b) using different methods.']","Figure 4 Boxplot showing the distribution of the average distances between control points after image registration. Each box is drawn from the first to the third quartile. The horizontal line represents the median value, and the triangle represents the mean. The whiskers indicate the minimum and maximum value of the distribution.",yes
PMC8605796,Figure_5,oa_package/5b/71/PMC8605796.tar.gz,"['The expression of ROR- t increased in DNCB-induced model mice with an alleviation in GA-treated group (A C).', 'The expression of SOCS3 showed an opposite change, it decreased in DNCB-induced model group but increased with GA-treatment (D F).', 'Effects of gallic acid on the Th17 mediated immune responses in DNCB induced atopic dermatitis-like mice.']","Figure 5 Effects of gallic acid on the Th17 mediated immune responses in DNCB induced atopic dermatitis-like mice. The ears were excised on day 43 and quantitative real time polymerase chain reaction was used to measure the mRNA expressions of ROR-t ( ) and SOCS3 ( ) in the ear tissues. Western blotting was used to measure the protein levels of ROR-t ( ) and SOCS3 ( ) in the ear tissues. The expressions were normalized to control ( and ). Mean SD was used to shown the data. ##p < 0.01, ###p < 0.001 compared to control, *p < 0.05, **p < 0.01, ***p < 0.001 compared to DNCB group. KruskalWallis test following by MannWhitney -tests.",yes
PMC9470140,Figure_4,oa_package/48/14/PMC9470140.tar.gz,"['Short Variants AnalysisThe short variants analysis (SNV) panel offers comprehensive annotations and several tools for inspection of SNV, insertions, deletions (indels), and frameshifts (A).', 'The first five columns provide standard annotations such as protein sequence, coding sequence, and exon annotations with outlinks to bioinformatic databases (A, columns 1-5).', 'The TCGA prevalence tool shows a histogram of the most frequently mutated amino acid positions within a gene in the TCGA data set (Figs 4C and A, column 7).', 'If no evidence is available, the database links are greyed out (A, column 8).', 'The SMART40 domain viewer shows the position of the alteration within the secondary structure of the protein and conserved domains (Figs 4D and A, column 9).', 'The MTP3D tool displays the special coordinates of the altered amino acid on protein structures of the PDB41 (Figs 4B and A, column 11).', 'FIG 4.']","FIG 4. Features of the single-nucleotide variant panel. (A) The different columns of the panel comprising annotation and links to interactive tools are highlighted by numbers: (1) protein name link to the UniProt entry; (2) variant allele frequency provided by the user; (3) protein mutation; (4) nucleotide alteration; (5) chromosome and exon number of the alteration; (6) TCGA amino acid position prevalence tool; (7) further information such as chromosomal coordinates or amino acid conservation score; (8) evidence inspection tool comprising the databases TCGA, ClinVar, gnomAD, and for BRCA genes, ARUP; (9) visualization of the alteration (red tag) within the 2-dimensional SMART domains of the protein; (10) biologic role of the gene according to KEGG pathways; (11) link to the viewer tool for analysis of alterations on three-dimensional protein structures; and (12) further links to other databases and NGS panels. (B) The viewer tool displays the altered amino acid as a sphere model on the structure with the highest resolution of the affected protein in cartoon representation. Different views such as stick and balls, spheres, or surfaces can be chosen, as well as other structures from the PDB database. In this example, the EGFR alteration C797S is displayed on PDB ID 6SBA. (C) The TCGA prevalence tool shows the frequency of alterations at a given amino acid position for the affected protein (gene ) and highlights the alteration provided by the user in red. In this example, the EGFR E746 position was altered and is highlighted as one of the 20 most common mutated amino acid positions of . (D) The SMART domain tool displays the location of the short nucleotide variant (red line) within the protein domains. In this example, the P53 R196* terminating mutation is shown within the DNA-binding domain of P53. NGS, next-generation sequencing; PDB, Protein Data Bank; TCGA, The Cancer Genome Atlas.",yes
PMC7565020,Figure_4,oa_package/9a/42/PMC7565020.tar.gz,"['ActinomycesSix biopsies from five patients, ranging in age from 54 to 78 years, showed infection with Actinomyces bacteria ().']",FIGURE 4 bacterial infection in an endometrial sample (original magnification 400) from a 57-year-old female who presented with postmenopausal bleeding. The bacteria are seen as the large clumped mass of basophilic filamentous structures.,yes
PMC4270442,Figure_1,oa_package/b2/42/PMC4270442.tar.gz,"['Treatment with the -secretase inhibitor DAPT reduced luciferase expression by approximately 80% (', 'Treatment with 2 structurally distinct BACE1 inhibitors, sI, and IV caused approximately 50% reduction in luciferase expression (', 'TAPI, a broad-spectrum metalloprotease inhibitor which reduces -secretase activity did not reduce but increased luciferase expression (', '', 'This enhanced luciferase expression approximately 10-fold (', 'Site directed mutagenesis of the APP-Gal4 vector to incorporate these point mutations resulted in a 2-fold increase in luciferase expression compared with non-mutated APP, whereas remaining sensitive to DAPT (']","Fig.1 APP-Gal4 driven luciferase gene reporter activity preferentially reports amyloidogenic processing in primary cortical neurons. Five DIV primary mouse cortical neurons chemically transfected with 0.5g pRC-APP-Gal4, pFR-luciferase, and pRL-TK-Renilla plasmids using 1L/well Lipofectamine-2k. (A) Differential effects of alpha, beta, and gamma secretase inhibitors on APP-Gal4 gene reporter indicate assay is sensitive to amyloidogenic processing. ** < 0.01, *** < 0.001, and **** < 0.0001 1-way ANOVA with Bonferroni posttest. (B) Promotion of luciferase signal following cotransfection with 0.5g adaptor protein Fe65. **** < 0.0001 Student t test. (C) Cotransfection with APP-Gal4 plasmid point mutated at Swedish K595N/M596L (APP695 numbering) compared with WT transfection caused increase in processing, sensitive to 10M DAPT. **** < 0.0001 1- way ANOVA with Bonferroni posttest. Abbreviations: ANOVA, analysis of variance; APP, amyloid precursor protein.",yes
PMC11747598,Figure_1,oa_package/d1/85/PMC11747598.tar.gz,"['Physical examination upon admission showed a massively distended abdomen with a diffuse, tough, non-tender mass with unclear margin filling all regions of the abdomen measuring about 40 30 cm (A).', 'A photograph of the patient showing a massive distended abdomen with traditional marks (A); a CT scan of abdomen and pelvis demonstrating a mixed soft tissue density mass occupying the entire abdomen (B).', 'A computed tomographic (CT) scan results of the chest, abdomen, and pelvis suggested a mixed soft tissue density mass occupying the entire abdomen and pelvis with displacement of visceral organs (']",Fig. 1 A photograph of the patient showing a massive distended abdomen with traditional marks (A); a CT scan of abdomen and pelvis demonstrating a mixed soft tissue density mass occupying the entire abdomen (B).,yes
PMC9475469,Figure_5,oa_package/4e/53/PMC9475469.tar.gz,['\nLgr5+ cells predominately undergo symmetric division while FXR activation but turn on asymmetric division while PPAR activation.'],"Figure 5 ISH for combined with IF for GFP and BrdU was performed to confirm expression and stem cell proliferation. Yellow circle depicts the symmetric division, both of the daughter cells with expression, and red circle depicts the asymmetric division, the stem cell with expression (white) and another daughter cell without expression. Scale bar represents 20 m. Quantification of symmetric or asymmetric division in GFP liver stem cell maintained in livers. n = 3 per group. Data were expressed as means SD.",yes
PMC6804320,Figure_2,oa_package/e3/82/PMC6804320.tar.gz,[''],"Fig. 2 CT sagittal view image depicting the foreign body trajectory, circled in red.",yes
PMC4801142,Figure_3,oa_package/2a/7a/PMC4801142.tar.gz,"['(A, B) Coronal thin maximum intensity projection (MIP) CT images showing: aorta (Ao), partially calcified pseudoaneurysm (P), dilated IVC and origin of the right renal artery from the pseudoaneurysm (arrow in B).']","Figure 3 ( ) Coronal thin maximum intensity projection (MIP) CT images showing: aorta (Ao), partially calcified pseudoaneurysm (P), dilated IVC and origin of the right renal artery from the pseudoaneurysm (arrow in ).",yes
PMC7541341,Figure_3,oa_package/08/25/PMC7541341.tar.gz,['.'],"Fig. 3. (A) Weight of hearts of 1-, 2-, 6-, 11- and 15-month-old wild-type (WT) and IF rats. Data are means+s.d. (B) Representative images of hematoxylin-eosin staining of heart sections of 6-month-old wild-type and IF rats. Scale bar: 50m. (C) Representative images of Massons trichrome staining of hearts of 11-month-old wild-type and IF rats. (D) Quantitative analysis of fibrosis area in left and right ventricle wall of 2-, 6- and 11-month-old wild-type and IF rats ( =3 in each group). Different letters indicate significant differences between groups. Data are means.d (A,D). Statistical significance was determined using an unpaired two-tailed Tukey's test ( <0.05).",yes
PMC11470440,Figure_1,oa_package/a8/f5/PMC11470440.tar.gz,['(A) Visualized ipsilateral pelvic lymphatic vessels and lymph nodes.'],Figure 1 Visualized ipsilateral pelvic lymphatic vessels and lymph nodes. Visualized cisterna chyli. Visualization of the total thoracic duct. . Thoracic duct disruption and lipiodol extravasation at the left venous angle (red circles).,yes
PMC11617238,Figure_3,oa_package/d1/32/PMC11617238.tar.gz,"['The surgeon and pathologist met via teleconference to discuss the findings using the visual pathology report ().', 'Three-dimensional (3D) images of the posterior (A) and right (B) aspects of a total laryngectomy specimen for Case #13, Illustrative Case #1.', '']","Fig. 3 Three-dimensional (3D) images of the posterior (A) and right (B) aspects of a total laryngectomy specimen for Case #13, Illustrative Case #1. Inlayed two-dimensional (2D) images (hematoxylin and eosin, H&E), corresponding to the alphabetic gross tissue cassettes, highlight sampling site N where the tumor is close (2mm) to the inked margin, but negative (4 magnification, top-right). A nearby separate section, O, showed tumor focally present at the inked surface, constituting a positive margin (20 magnification, bottom-right).",yes
PMC7554859,Figure_5,oa_package/11/3e/PMC7554859.tar.gz,"['A,B show in transillumination (A) and in polarized light (B) the cortico-medullary transition of a kidney after CM administration.', 'B displays the birefringence of the fibrous collagen constituents of connective tissues accompanying vascular walls (white fiber nets), documenting the effectivity of the polarized light illumination.', 'Cortico-medullary transition of a kidney without ligature of supplying vessels after CM application in transillumination (A) and in polarized light (B).']",Figure 5 Cortico-medullary transition of a kidney without ligature of supplying vessels after CM application in transillumination ( ) and in polarized light ( ). Primary magnification 1:100.,yes
PMC2965244,Figure_5,oa_package/ef/00/PMC2965244.tar.gz,"['Rescue mice have decreased COX activity and decreased ATP productionWe performed COX-succinate dehydrogenase (COX-SDH) double staining on fresh-frozen sections of hearts from 10-week-old cardiomyopathy, rescue and control animals (A).', '.', 'No changes in citrate synthase activity (CS) were found, indicating that the transgenic animals had similar mitochondrial mass as control animals (B).', 'Biochemical measurements demonstrated an isolated COX deficiency at 12 weeks and tendency to reduction at 52 weeks in relation to CS activity (B).', 'We measured the MAPR in freshly isolated heart mitochondria, both in relation to CS activity (C) and in relation to heart tissue mass (Before surgery, all patients received at least 3 months of formal physical therapy or a home exercise program as well as a subacromial corticosteroid injection.']","Figure 1. Flowchart demonstrating patient selection process for study inclusion. MRI, magnetic resonance imaging.",yes
PMC4126113,Figure_4,oa_package/ac/41/PMC4126113.tar.gz,"['On sagittal view, it appeared as a narrow hyperechoic, curved structure casting an acoustic shadow[56789] [].', 'The scan shows hyoid bone (arrowheads) with posterior acoustic shadowingLeft parasagittal view through thyrohyoid membrane using a linear transducer.', '[56789] We could visualize epiglottis in 90% volunteers in transverse view and 70% of volunteers in parasagittal view [].']","Figure 4 Left parasagittal view through thyrohyoid membrane using a linear transducer. The scan shows epiglottis (EPI), preepiglottic space (PES), hyoid bone (HY), strap muscles (SM), air-mucosal interface (arrowheads), and thyroid cartilage (TC)",yes
PMC10292048,Figure_3,oa_package/74/c3/PMC10292048.tar.gz,"['CTA: computed tomography angiographyMRI of the heart with and without contrast: (a) four-chamber view, (b) T2 Haste, (c) T1 pre-contrast, and (d) T1 post-contrast (Gd).']","Figure 3 MRI of the heart with and without contrast: (a) four-chamber view, (b) T2 Haste, (c) T1 pre-contrast, and (d) T1 post-contrast (Gd). Pedunculated left atrial mass corresponding with the mass previously seen on CT demonstrating low signal on T1, high signal on T2, and heterogenous enhancement on T1 C+ (Gd) (arrows). MRI: magnetic resonance imaging, CT: computed tomography, Gd:gadolinium",yes
PMC8392744,Figure_3,oa_package/b1/72/PMC8392744.tar.gz,"['03%, respectively ().', '(a) AI-CAD did not detect microcalcifications on postoperative screening mammography in a 40-year-old woman.']","Figure 3 ( ) AI-CAD did not detect microcalcifications on postoperative screening mammography in a 40-year-old woman. She had a history of breast-conserving surgery on her right breast. ( , ) Left craniocaudal and mediolateral oblique routine mammography showed grouped microcalcifications (arrows). ( ) The left craniocaudal magnification and compression view showed grouped amorphous microcalcifications (arrow). The radiologists categorized the microcalcifications as category 4a. ( ) The malignancy rated by AI-CAD was 1.21% (low, arrow). The adjusted category was downgraded to 3 for all three cutoff values (2%, 10%, and 38.03%). Fibrocystic changes with microcalcifications were confirmed by mammography-guided localization and excisional biopsy. AI-CAD, artificial intelligence-based computer-aided diagnosis.",yes
PMC4916179,Figure_1,oa_package/59/f3/PMC4916179.tar.gz,['Opioid systems effects on immune responses.'],"Figure 1 . Opioid receptors are abundantly expressed on various immune cells and immune function is influenced by these receptors activation/inhibition. Numerous immune factors and immune responses are significantly dampened following opioid receptors activation. Opioid and chemokine receptors interaction on the immune cells surface regulates immune responses; Activation of opioid receptors by their ligands desensitizes chemokine receptors and also activation of chemokine receptor by its ligand desensitizes opioid receptor. Importantly, opioid receptors antagonists stimulates cellular immune responses and shifts immune responses to a Th1 pattern.",yes
PMC6628046,Figure_2,oa_package/03/ab/PMC6628046.tar.gz,"['The increase in the CellROX Green fluorescence level in transfected SOD1G85R cells was significantly decreased by p-CA treatment (A,B).', 'p-CA treatment also obviously decreased the level of MitoSOX Red fluorescence (C,D).', 'p-CA significantly attenuated the signal intensity of hydroxy radicals (E,F).', 'p-CA reduced mutant-SOD1-related oxidative stress.']","Figure 2 p-CA reduced mutant-SOD1-related oxidative stress. ( ) Confocal imaging of CellROX in N2a cells transfected with mCherrySOD1 with p-CA for 24 h. ( ) Quantified analysis of CellROX using Image J. ( ) Confocal imaging of MitoSOX in N2a cells transfected with mCherrySOD1 with p-CA for 24 h. ( ) Quantified analysis of MitoSOX using Image J. ( ) Typical spectra of DMPO-OH spin generated from H O plus Fe in the absence (control) or presence of p-CA. ( ) The amount of hydroxy radicals was semi-quantitatively measured as the formation of DMPO-OH spin adducts by ESR spectrometry. Differences were evaluated by one-way ANOVA (mean SEM, = 3). *** < 0.001 vs. SOD1 , < 0.01, < 0.05 vs. SOD1 . Scale bar: 10 m. p-CA: p-coumaric acid.",yes
PMC8983543,Figure_1,oa_package/03/6f/PMC8983543.tar.gz,"['Papillary neoplasms in the WHO classification of breast tumors 1 provides an overview on papillary neoplasms listed by the 2019 WHO classification of breast tumors with emphasis on special features and differential diagnosis.', 'Staining patterns of myoepithelial markers and hormone receptors in various papillary lesions of the breast.', 'com)Intraductal papillomaIntraductal papillomas are benign intraluminal proliferations consisting of arborizing fibrovascular cores covered by a population of basal and luminal cells [85].', ' 11).', '1Micropapillary and papillary DCIS in a small duct (A) adjacent to invasive papillary carcinoma (not shown).', 'Immunohistochemistry for CK5 shows the lack of basally differentiated cells (D)Invasive micropapillary carcinomaInvasive micropapillary carcinoma (IMPC) is characterized by clusters of cells within clear spaces showing an inside-out pattern.', ' 12) [2].', '2Invasive micropapillary carcinoma characterized by an inside-out growth pattern (A) highlighted by EMA immunoreactivity (B).', 'In contrast, EMA is negative in invasive NST carcinomas with retraction clefts (C, D)IMPC does not show pathognomonic mutations or translocations but distinctive complex patterns of copy number alterations as compared to NST carcinomas, such as 16q losses and 8q, 17q, and 20q gains [55].', ' 13).', '3Secretory carcinoma with papillary architecture in a core needle biopsy (A).', 'Solid areas (B) in transition to microcystic areas with presence of intraluminal eosinophilic secretion (C) and prominent papillary architecture (D) are typical cytoarchitectural featuresAdenomyoepithelioma with papillary growth patternAdenomyoepithelioma (AME) of the breast is characterized by an epithelial-myoepithelial phenotype with heterogeneous architecture including a predominant papillary pattern [29].', ' 14).', ' 15), it is diffusely expanded in papillary AME [33].', '4Adenomyoepithelioma with partial papillary growth pattern showing multinodular architecture with thick fibrovascular septa (A) and presence of expansive nodules of myoepithelial cells (*).', 'The myoepithelial nodules show a mixture of glandular adenosis-like and spindle cell growth patterns (B) Proliferation of myoepithelial cells is confirmed by CK14 (C), calponin (D), and p63 immunostaining (E)5Intraductal papilloma with myoepithelial hyperplasia showing an area with increased cellularity (asterisk) (A) characterized by expansion of myoepithelial cells (B).', 'The retained myoepithelial layer and the expansion of the myoepithelial compartment is highlighted by p63 (C) and CD10 (D) immunoreactivityNipple adenomaNipple adenoma (NA) is a benign tumor originally described as florid papillomatosis of the nipple [78].', ' 16).', '6Nipple adenoma presenting as a mass at the dermo-epidermal junction with a large duct at the periphery (A).', 'Focally, usual ductal hyperplasia with papillary architecture is evident (B)Non-neoplastic lesions with papillary structuresA variety of non-neoplastic lesions may show a papillary or micropapillary pattern.']","Fig. 1 Staining patterns of myoepithelial markers and hormone receptors in various papillary lesions of the breast. The presence of myoepithelial markers is illustrated by bordeaux brown dots. Myoepithelial cells may be present or not in the peripheral wall and/or centrally in association with the branching fibrovascular cores illustrated in pink. In the same fashion, the bright red dots highlight the expression of hormone receptors in the lining epithelium. The lining epithelium is illustrated with a continuous blue line which depending on the degree of proliferation, and malignancy of the lesion becomes thicker with almost disappearance of the pink fibrovascular cores. Each combination of staining patterns is associated to specific lesions for which the B category on CNB, and the differential diagnosis is proposed. (This figure was created with )",yes
PMC7409655,Figure_10,oa_package/80/f8/PMC7409655.tar.gz,[],"Fig. 10 Microglia uptake scFvMC1 in vivo ( P301S were injected intracranially in the CA1 quadrant of the hippocampus using AAV5-GFAP-scFvMC1. Upper panels: Representative confocal images of the cortex: scFvMC1 (Myc, red) co-localizes within the microglia (Iba1, green); nuclei stained with DAPI (blue). Lower panels: higher magnification to better visualize scFvMC1 in microglia positive cells (Zeiss880 confocal laser microscope; upper panels, scale bar: 20m; lower panels, scale bar: 10m). Flow cytometry on microglia isolated from adult P301S mice intracranially injected with AAV5-GFAP-scFvMC1 or AAV5-null ( ) Gating strategy (live, singlets) for subsequent selection of microglia. ( ) Gating strategy to isolate microglia from other monocytes. Representative plot showing microglia population: CD11b and CD45 ; near-complete absence of macrophages: CD11b and CD45 . ( ) Microglia extracted from P301S mice, treated (blue) or not (red) with scFv-MC1: upon permeabilization, anti-Myc-647 detects scFvMC1 in microglia of treated mice (blue)",yes
PMC11299127,Figure_2,oa_package/4c/fd/PMC11299127.tar.gz,"['Massive hematoma(A)-(B) A massive hematoma (red arrow) measuring 105 mm 55 mm in the subchorionic space, with a thickness of 22 mm, located directly beneath the umbilical cord attachmentPathological findings of the placenta (A)-(B) Pathological examination of the placenta revealed a hematoma beneath the chorionic plate, surrounded by numerous microinfarcts.']","Figure 2 Massive hematoma (A)-(B) A massive hematoma (red arrow) measuring 105 mm 55 mm in the subchorionic space, with a thickness of 22 mm, located directly beneath the umbilical cord attachment",yes
PMC9559604,Figure_4,oa_package/f4/53/PMC9559604.tar.gz,"['There was no significant change in spreading of NP-tau pathology into the contralateral hemisphere of female mice and the contralateral HC of male mice (, D, E, and I).', 'Chronic TREM2 agonist antibody administration increases peri-plaque NP-tau pathology, neuritic dystrophy, and loss of peri-plaque synaptic punctaTo confirm that cortical NP-tau pathology was increased after the TREM2 antibody treatment independently of the number of A plaques, we performed confocal analysis and quantified the amount of NP-tau surrounding individual X34+ A plaques.', 'We confirmed on a per-plaque level that NP-tau pathology that represents seeding was increased in the ipsilateral HC of the TREM2 antibody treated mice (, B and H).', 'We did not see a significant difference in the ipsilateral cortex and contralateral regions of our mice, possibly due to lower extent of seeding and spreading NP-tau levels in those regions (, G, I, and J).', 'In the ipsilateral cortex and HC, there was increased BACE1+ neuritic dystrophy around A plaques in the TREM2 antibody treated mice (, A, C, and D).', 'This effect was not seen in the contralateral brain regions (, E and F).', 'These results suggest that in contrast to acute pharmacological stimulation of TREM2, chronically agonizing TREM2 with an anti-TREM2 antibody leads to an increase in A -associated dystrophic neurites that appear to provide a better substrate for tau seeding and spreading (, A J).']","Figure 2. Representative images of A plaques in 5XFAD mice either treated with the IgG control antibody or the TREM2 antibody. Scale bar, 100 m. Quantification of A staining in the ipsi- and contralateral cortices (B and D) and hippocampi (C and E) in female mice. Quantification of A staining in the ipsi- and contralateral cortices (F and H) and hippocampi (G and I) in male mice. Triangle symbol represents female mice, and square symbol represents male mice either treated with the IgG control antibody ( = 14 female, = 12 male) or the TREM2 antibody ( = 13 female, = 14 male). Data are presented as mean SEM. Scale bar, 100 m. Significance was determined using a Students test. ns, P > 0.05.",yes
PMC9586787,Figure_3,oa_package/43/6e/PMC9586787.tar.gz,"['In the 2-year follow-up call after discontinuing minocycline treatment, the dark blue pigmentation in the gums remained ().', '002"" position=""float""/>2-year follow-up after discontinuation of minocycline, dark blue pigmentation is found in the maxillary gingival margins.']","Figure 3 2-year follow-up after discontinuation of minocycline, dark blue pigmentation is found in the maxillary gingival margins.",yes
PMC4496390,Figure_10,oa_package/35/72/PMC4496390.tar.gz,[],"Figure 10 Adenoviral delivery of Fgf21 reversed the impaired liver regeneration in hPPAR mice Adenovirus-mediated expression of FGF21 in hPPAR mouse livers was accomplished via tail vein injection of Ad-Fgf21 or control vector (Ad-Control) followed by PH surgery. Western blot analysis revealed restoration of FGF21 protein levels in hPPAR mice was similar to that in WT (A). Liver-to-body weight ratios were recorded (B). Representative images of hPPAR livers with Ad-control or Ad-Fgf21 injection harvested 2 and 7 days after PH (C). Representative images of H&E staining (10) of hPPAR liver sections with and without Ad-Fgf21 injection harvested 2 and 7 days after PH (D). All values represent mean standard deviation, = 5; <0.05, student's test, hPPAR with Ad-control WT with Ad-Control.",yes
PMC10374160,Figure_5,oa_package/72/06/PMC10374160.tar.gz,[],"Fig. 5. Loss of reverses gliosis and synaptic plasticity deficits in tau mice. ( ) Representative images of GFAP (green), Iba1 (red), and DAPI (blue) staining from 7-mo-old WT, P301S, and P301S; mice. ( ) Quantification of Iba1 intensity [one-way ANOVA, F (2, 93) = 30.78, < 0.0001, post hoc Dunnett, **** < 0.0001, n = 7 to 9 images/mouse from four mice/genotype). ( ) Quantification of GFAP intensity [one-way ANOVA, F (2, 78) = 26.39, < 0.0001, post hoc Dunnett, **** < 0.0001, * = 0.014, n = 5 to 7 images/mouse from four mice/genotype]. ( ) Schematic of electrophysiological recordings from acute brain slices. ( ) PPF showing fEPSP slope as a function of increasing interstimulus intervals from 30 to 300 ms from WT, P301S, and P301S; brain slices [two-way ANOVA, F (2, 2055) = 324.4; post hoc Tukey, main genotype effects, WT vs. P301S: < 0.0001; WT vs P301S; : < 0.0001; P301S vs. P301S; : < 0.0001; n = 33 to 62 slices/genotype from 4 to 7 mice/genotype). ( ) LTP induced by theta burst stimulation, showing fEPSP slope from WT, P301S, and P301S; slices [two-way ANOVA, F (79, 10320) = 5736; post hoc Dunnett, main genotype effects, WT vs. P301S: < 0.0001; P301S vs. P301S; : < 0.0001; n = 29 to 60 slices from 4 to 7 mice/genotype]. ( ) Representative images of the hippocampus stained for synaptophysin (red) from 7-mo-old WT, P301S, and P301S; mice. ( ) Quantification of synaptophysin intensity [one-way ANOVA, F (2, 68) = 13.66, < 0.0001, post hoc Dunnett, **** < 0.0001, * = 0.0316, n = 5 to 7 images/mouse from four mice/genotype].",yes
PMC6702254,Figure_21,oa_package/ea/27/PMC6702254.tar.gz,[],"Fig. 21 Solitary left pelvic kidney in a foetus at 20weeks gestation, following termination of pregnancy for presumed renal aplasia. Post-mortem imaging was obtained 3days after delivery. Transverse post-mortem ultrasound image ( ) demonstrates a soft tissue mass in the left iliac fossa which lacks the normal expected renal corticomedullary differentiation but appears to have a demonstrable hilum (solid arrow). The same finding is demonstrated on the corresponding post-mortem T2 weighted MRI study, obtained in axial plane ( ) and also on photographs obtained at autopsy ( ), acquired after careful dissection of the anterior abdominal wall. The extracted soft tissue mass ( ) was confirmed to be renal in origin",yes
PMC6849156,Figure_1,oa_package/2b/28/PMC6849156.tar.gz,"['The scan showed a vastly dilated stomach with a transition line between the antral and fundic air bubbles due to a volvulus ().', 'CT showing the gastric volvulus.', '']",Fig. 1 CT showing the gastric volvulus.,yes
PMC4008056,Figure_2,oa_package/f0/6e/PMC4008056.tar.gz,[],FIGURE 3 Onychomycosis,yes
PMC11172438,Figure_2,oa_package/82/61/PMC11172438.tar.gz,"['A small gap ought to be visible ().', 'Fluoroscopic check of correct attachment of Amplatzer(-like) occluders (frontal view).']",Figure 2 (frontal view). A small gap (white arrow) between pusher screw collar and screw nut on the device guarantees that the occluder is still safely attached but will be easily detachable once positioned. The inserts show screw positions that should be corrected by turning the pusher cable either clockwise (if too loose) or counterclockwise (if too tight) before advancing the occluder out of the sheath.,yes
PMC7011484,Figure_2,oa_package/49/40/PMC7011484.tar.gz,"['It was hypointense on T1-weighted images and hyperintense on T2-weighted images ().', '001""/>Case 1: sagittal left shoulder MRI showing a 6 cm intramedullary lesion involving the proximal left humerus with associated periostitis.']",Figure 2 Case 1: sagittal left shoulder MRI showing a 6cm intramedullary lesion involving the proximal left humerus with associated periostitis. The lesion was hypointense on T1-weighted images (a) and hyperintense on T2-weighted images (b).,yes
PMC8456088,Figure_4,oa_package/5d/ce/PMC8456088.tar.gz,[],"Figure4 PD-L1 IHC staining for B2 and B3 samples in the cluster C4. Top, hematoxylin and eosin (H&E) staining of the tissues. Bottom, IHC staining of PD-L1 in the cells using antibody 22C3 (Dako). Brown cells are PD-L1 staining cells. The H&E staining was used to estimate the tumor cell percentage (TCP). The expression level of PD-L1 protein is determined by the tumor proportion score (TPS), which is further divided into three types: High expression (TPS 50%), Positive (1% TPS < 50%) and Negative (TPS < 1%).",yes
PMC9491115,Figure_4,oa_package/a2/0e/PMC9491115.tar.gz,[],Figure4 The KaplanMeier PD/RFS curve of all 32 patients. The KaplanMeier OS curve of all 32 patients.,yes
PMC5121547,Figure_1,oa_package/2a/0f/PMC5121547.tar.gz,['Virchows Arch20024415195221244768424WangHWangXJuYWangJZhangXChengYClinicopathological features and prognosis of pseudomyxoma peritoneiExp Ther Med201471851902434878725LamKLoCWatMFanSTMalignant insulinoma with hepatoid differentiation: a unique case with alpha-fetoprotein productionEndocr Pathol2001123513541174005626HughesKKeltySMartinRHepatoid carcinoma of the pancreasAm Surg200470103010331558652127KellyPJSpenceRDasariBVBurtADTaylorMLoughreyMBPrimary hepatocellular carcinoma of the pancreas: a case report and review of the heterogeneous group of pancreatic hepatoid carcinomasHistopathology201260101210152232068128PanerGPThompsonKSReyesCVHepatoid carcinoma of the pancreasCancer200088158215891073821629TakaiEYachidaSGenomic alterations in pancreatic cancer and their relevance to therapyWorld J Gastrointest Oncol201572502582648387930TsimberidouAMIskanderNGHongDSWhelerJJFalchookGSFuSPersonalized medicine in a phase I clinical trials program: the MD Anderson Cancer Center initiativeClin Cancer Res201218637363832296601831JiaoYYonescuROfferhausGKlimstraDSMaitraAEshlemanJRWhole-exome sequencing of pancreatic neoplasms with acinar differentiationJ Pathol201423242843524293293Cytology of pancreatic mass obtained via fine needle aspiration on EUS.'],"Fig. 1 Cytology of pancreatic mass obtained via fine needle aspiration on EUS. The fine needle aspirate of the tumor showed clusters of malignant epithelioid cells with marked nuclear variation. Pap stain. Original magnification, 40.",yes
PMC4429024,Figure_3,oa_package/ca/29/PMC4429024.tar.gz,"['In fact, the frontal bone was visually much more porous, showing less trabecular bone and larger lacunae, at P10 in the MT group comparing to the WT group while the parietal bone was visually similar between the MT and WT ().', 'g003Visual comparison between the frontal (A) and parietal (B) bone in the WT and MT mice at P10 in two samples from each group.', 'The fact that frontal bone was visually much more porous at P10 in the MT group comparing to the WT group () explains that the differences in the elastic modulus of the frontal bone in the WT and MT at P10 is probably a reflection of not only differences in the tissue properties, but also variations in structural properties (bone architecture).']",10.1371/journal.pone.0125757.g003,yes
PMC10580581,Figure_4,oa_package/9a/3f/PMC10580581.tar.gz,"[' 4).', '\nA follow-up CT scan 6 months after discharge.', 'It shows a mosaic attenuation pattern, (hyperinflation (yellow circle) and ground glass opacity (red circle)), atelectasis (blue circle), and interlobular septal thickening (yellow arrow) in both lungs\nA follow-up CT scan 19 months after discharge.']","Fig. 4 A follow-up CT scan 6 months after discharge. It shows a mosaic attenuation pattern, (hyperinflation (yellow circle) and ground glass opacity (red circle)), atelectasis (blue circle), and interlobular septal thickening (yellow arrow) in both lungs",yes
PMC2185514,Figure_5,oa_package/dc/92/PMC2185514.tar.gz,"['In H E stained sections, normal cornea was separated from the lens by a distinct endothelial layer and the corneal epithelium showed well defined, stratified squamous epithelium with a smooth surface (A, A).', 'The defective eye also had a narrow anterior chamber and showed either partially formed or discontinued corneal endothelium (B,C).', ""Unlike the thin monolayer of lens epithelial cells in the lens' anterior surface of wild-type mice, the anterior region of the lens had abnormal multilayer cells in transgenic mice ()."", 'Histopathologic findings of normal eye in wild-type mouse and the defective eye in transgenic mouse at two months of ageStaining was performed with H E in each 4 m thick paraffin section.', 'As shown in the wild-type mouse of , the cornea was separated from the lens by a distinct endothelial layer; this was also the case in the seven-month-old wild-type mouse (E).']",Figure 5 Histopathologic findings of normal eye in wild-type mouse and the defective eye in transgenic mouse at two months of age,yes
PMC5037396,Figure_1,oa_package/c7/5f/PMC5037396.tar.gz,"['ResultsC57BL/6 2GPI deficient mice have significantly higher levels of inflammatory cytokines compared to C57BL/6 wild type (WT) mice at 2 and 6 h post LPS challengeSignificantly higher levels of TNF were noted at both 2 and 6 h post LPS injection in the 2GPI / mice compared to the WT mice (A,B).', 'Significantly higher levels of IL-6, IFN- , MIP-1 , and Eotaxin-1 (C G) were detected in the 2GPI / compared to WT mice 6 h post intravenous LPS administration.', 'Levels of cytokines noted in A G were the same in WT and 2GPI / mice given pyrogen free saline.', '(A G) 2GPI deficient mice had a significant increase in plasma inflammatory cytokines at 2 h and 6 h time points following LPS challenge.']","Figure 1 ( ) 2GPI deficient mice had a significant increase in plasma inflammatory cytokines at 2h and 6h time points following LPS challenge. ( ) TNF at 2h ( ) TNF at 6h. For IL-6, IFN, MIP-1, MIP-1 and Eotaxin ( ) at 6h. ()=WT=Wild Type mice, ()=2GPI/= -glycoprotein I deficient mice. Mann-Whitney test n=5 *p<0.05, **p<0.01, ***p<0.001.",yes
PMC11323557,Figure_2,oa_package/12/64/PMC11323557.tar.gz,"['Imaging examination: The chest CT scan () revealed a nodular soft tissue density shadow in the bronchus of the right middle lobe, accompanied by bronchial obstruction and atelectasis of the right middle lobe.', 'Female, 57 years old, pulmonary angiomatoid fibrous histiocytoma.']","Figure 2 Female, 57years old, pulmonary angiomatoid fibrous histiocytoma. whole body MIP, axial PET, axial CT, axial fusion. The 18F-FDG PET/CT revealed right middle lobe atelectasis and a 2.2cm2.5cm soft tissue density nodule within the bronchus of the right middle lobe of the lung. The density was uneven, with clear boundaries, and there was increased glucose metabolism, with an SUVmax of 11.2 (arrow).",yes
PMC4022760,Figure_1,oa_package/26/59/PMC4022760.tar.gz,"['Surgery was planned with a preoperative diagnosis of a retroperitoneal tumor ().', ""Part 2: pathogenesis, Castleman's disease, and pleural effusion lymphomaLancet Infect Dis200223443521214489712OksenhendlerEDuarteMSoulierJCacoubPWelkerYCadranelJMulticentric Castleman's disease in HIV infection: a clinical and pathological study of 20 patientsAIDS1996106167892425313HerradaJCabanillasFRiceLManningJPughWThe clinical behavior of localized and multicentric Castleman diseaseAnn Intern Med1998128657662953794014CroninDMWarnkeRACastleman disease: an update on classification and the spectrum of associated lesionsAdv Anat Pathol2009162362461954661115McClainKLNatkunamYSwerdlowSHAtypical cellular disordersHematology Am Soc Hematol Educ Program200428329615561688T1-weighted magnetic resonance images showing a well-defined enhanced mass, about 5 cm 4 cm 2.""]","Fig. 1 T1-weighted magnetic resonance images showing a well-defined enhanced mass, about 5 cm 4 cm 2.8 cm, with hypervascular and heterogenic characteristics in the right retroperitoneum, suggesting rule out Castleman's disease. (A) Coronal view, (B) Transverse view.",yes
PMC4993389,Figure_7,oa_package/bf/f5/PMC4993389.tar.gz,"['In addition, slightly enhanced plasma levels of IL-6 (217 105 pg/ml), IL-12p70 (15 3 pg/ml), TNF (48 7 pg/ml) and MCP-1 (160 25 pg/ml) were present (A).', 'In contrast, immunocompetent BALB/c wild-type mice produced significantly increased levels of IFN (191 70 pg/ml) in addition to MCP-1 (197 81 pg/ml) exclusively at day 3 post infection while IL-12p70, IL-6 and TNF were not detectable at all in these mice (A) and GM-CSF was not significantly elevated during the course of infection (Microscopic pathology: H-E stain at 200x at the lesion of the right femur: the specimen demonstrated large osteoclasts and uniform mononuclear cells with ovoid nuclei, giant cells containing about 50 to 100 nuclei, and ill-defined cytoplasm with very few intercellular collagen.']","Figure 7 Microscopic pathology: H-E stain at 200x at the lesion of the right femur: the specimen demonstrated large osteoclasts and uniform mononuclear cells with ovoid nuclei, giant cells containing about 50 to 100 nuclei, and ill-defined cytoplasm with very few intercellular collagen.",yes
PMC11311325,Figure_5,oa_package/22/21/PMC11311325.tar.gz,"['Evaluation of the Colocalization of ATTR and AANF Using Single and Double IHCIn the single immunohistochemistry (IHC) results, some amyloid nodules consisted of both ATTR and AANF deposits; however, the positive areas of ATTR and AANF were observed separately, with no clear colocalization (a d).', 'However, specimens stained with double IHC showed no clear colocalization of ATTR and AANF (e,f).', 'Representative microphotographs of nodular deposits in the LA evaluated using double IHC for ANF and transthyretin.']","Figure 5 Representative microphotographs of nodular deposits in the LA evaluated using double IHC for ANF and transthyretin. ( ) Patient 10. ( , ) pCR staining under bright field ( ) and polarized light ( ). Single IHC for ANF ( ) and transthyretin ( ). Double IHC for ANF and transthyretin ( , ). ( ) Nodular lesions observed in the right half are positive for ANF but negative for transthyretin, whereas those observed in the left half are negative for ANF but positive for transthyretin. ( , ) Double IHC shows no clear colocalization of the two (ANF, brown; transthyretin, green) in the myocardial layer ( ) or endocardial interstitium ( ). Scale bar = 100 m ( ).",yes
PMC7397738,Figure_4,oa_package/4f/44/PMC7397738.tar.gz,"['475 10( 3) mm(2)/sec) (a d).', 'Axial CT scan orbit (a) and sagittal T1 fat suppressed post contrast (b,c) and axial DWI (D) images showing solid lobulated extra-conal orbital lesion arising superiorly along the orbital roof and nasal infiltrating the recti muscles and superior oblique muscle.', 'Pathological examination revealed a highly vascular soft tissue of poorly differentiated malignant cells infiltrating the adipose tissue with few eosinophils suggestive of malignant orbital tumor in the right eye, The tumor cells expressed the following immunohistochemical markers: CD45, 34, 43, 117, Lysozyme, CD68 (KP1) and Myeloperoxidase indicating myeloid lineage but did not show expression of CD20 and CD3 (', 'Diffusion weighted image (DWI) demonstrating high signal at the site of the mass indicating restricted diffusion, likely reflecting increased cellularity ().']","Fig. 4 Axial CT scan orbit (a) and sagittal T1 fat suppressed post contrast (b,c) and axial DWI (D) images showing solid lobulated extra-conal orbital lesion arising superiorly along the orbital roof and nasal infiltrating the recti muscles and superior oblique muscle. The lesion presents marked restricted water diffusion on DWI (c) indicating high degree of cellularity. The lesion abutting the lacrimal gland with no definite line of separation.",yes
PMC4494798,Figure_5,oa_package/77/7d/PMC4494798.tar.gz,"[' 5a).', ' 5b).', ' 5c).', 'Lung and brain parenchyma of BALB/c mice infected with Toxocara canis.', 'Bar = 20 mThe histopathological evaluation of the brains of uninfected animals showed a normal histological appearance (', ' 5d).', ' 5e), and scattered larvae were visualized in the cerebrum and brainstem (', ' 5f).']","Fig. 5 Lung and brain parenchyma of BALB/c mice infected with . Control group: normal lung parenchyma. H&E staining. =50m. 14days post-infection (p.i.): thickening of the septum due to inflammatory infiltrate ( ) and hemorrhagic areas (*). H&E. =50m. Higher magnification of the previous figure (14days p.i.) showing inflammatory infiltrates consisting of eosinophils, lymphocytes, macrophages and the presence of hemorrhagic areas (*). H&E. =20m. Control group: normal brain parenchyma. H&E. =20m. 7days p.i.: brain with hemorrhagic cavities (*). H&E. =20m. 14days p.i.: presence of larvae in the brain ( ). H&E. =20m",yes
PMC9671740,Figure_3,oa_package/52/73/PMC9671740.tar.gz,"['Supplementary : representative micrographs.', '002"" position=""float""/>Inhibition of miR-182/183 cluster improves SZ at different levels: (a) miR-182/183 cluster transfection efficiency (n = 8/group); (b) open field test (left), PPI test (middle), and Morris water maze test (right) in SZ rats (n = 8/group); (c) representative micrographs showing Nissl body in hippocampal tissues from SZ rats.']","Figure 3 Inhibition of miR-182/183 cluster improves SZ at different levels: (a) miR-182/183 cluster transfection efficiency ( = 8/group); (b) open field test (left), PPI test (middle), and Morris water maze test (right) in SZ rats ( = 8/group); (c) representative micrographs showing Nissl body in hippocampal tissues from SZ rats. MK-801-exposed hippocampal neurons were treated with miR-182/183 mimic or AntimiR-182/183; (d) expression of miR-182 and miR-183 in the MK-801-exposed hippocampal neurons determined by RT-qPCR; (e) quantitative analysis of apoptosis of MK-801-exposed hippocampal neurons detected by Hoechst 33342 staining. The data in the figure were measurement data, expressed as the mean standarddeviation, and analyzed by one-way ANOVA. < 0.05 the AgomiR control or miR-182/183 mimic-NC control; < 0.05 . the scrambled control or AgomiR control group. The cell experiments were repeated three times independently.",yes
PMC10017268,Figure_3,oa_package/52/c9/PMC10017268.tar.gz,"['Venography was then performed, which revealed numerous vessels that had developed as collateral blood vessels ().', '.']",Figure 3. Venographic findings at the beginning of endovascular treatments. Venography from the distal external iliac vein at the 30 right anterior oblique view shows numerous collateral vessels (arrows). Note that it could not be confirmed which vessel was connected to the common iliac vein from the external iliac vein.,yes
PMC10742688,Figure_6,oa_package/06/0f/PMC10742688.tar.gz,"['Case 2A biopsy of a large osteolytic bone lesion in the right temporomandibular joint (caput mandibulae) of a 24-year-old man showed a giant cell-containing tumor with multicystic spaces ().', 'Multicystic lesion with osteoclast-like giant-cells.']","Figure 6 Multicystic lesion with osteoclast-like giant-cells. The cystic spaces are sometimes filled with blood and fibrin ( ); hematoxylin and eosin staining, original magnification 100). More solid areas in the lesion were observed, with osteoid, osteoclasts-like giant cells and spindle cells with more pronounced atypia and nuclei varying in size and shape ( , ); hematoxylin and eosin staining, original magnification 200).",yes
PMC8955692,Figure_2,oa_package/8c/a5/PMC8955692.tar.gz,"['[IHC, 400 ](a-d) Ependymoma and chordoma: (a) Squash cytology of ependymoma.']","Figure 2 (a-d) Ependymoma and chordoma: (a) Squash cytology of ependymoma. : Ependymal tumors cells. [H&E, 400]. (b) Histopathology of ependymoma: ependymal canal. : Ependymal pseudorosette. [H&E, 400]. (c) Squash cytology of chordoma. [MGG, 400] : Polygonal tumors cells. [H&E, 400]. (d) Histopathology of chordoma. [H&E, 400]",yes
PMC8635412,Figure_3,oa_package/f5/2d/PMC8635412.tar.gz,"[' 3a) with many exhibiting much higher activity than UBE3A T485A (', ' 3b).', ' 3c), demonstrating that reporter activation resulted from increased ubiquitin ligase activity.', 'Characterization of hyperactivating mutations in UBE3A.', 'We next tested the ability of gain-of-function mutants to self-ubiquitinate, a process shown to be accelerated for the hyperactive UBE3A T485A mutation12 as well as for a variety of active HECT domain ubiquitin ligases36,37.', ' 3d).', ' 3b and Supplementary Data 1) whereas Q588P and Q588R abolished UBE3A ubiquitin ligase activity (Supplementary Data 1).', ' 3b, c.']","Fig. 3 Characterization of hyperactivating mutations in UBE3A. Schematic of UBE3A showing the positions of hyperactivating mutations identified in our screen. , Normalized BAR responses of hyperactivating mutations with ( ) and without ( ) a ligase-dead (LD; C820A) mutation. BAR responses in were re-plotted from the initial screen in Fig. and are shown as the meanSE. Exact numbers of experiments and -values for variants with ligase activity are provided in Supplementary Data . All variants for the LD were tested in three independent experiments and -values calculated using a One-sample -test (two-tailed) with BenjaminiHochberg multiple comparisons correction (FDR=0.05). V133I LD, *** =8.6810 ; Q196P LD, *** =3.5610 ; S33T LD, *** =1.4910 ; E388D, *** =1.4410 ; L390F LD, *** =1.2010 ; D415E LD, *** =1.6810 ; T485A LD, *** =2.3610 ; R516Q LD, *** =1.5510 ; R516W LD, *** =1.5910 ; A521T LD, ** =0.0014; Q588E LD, *** =1.2510 ; N692S, *** =1.3710 ; K701I, *** =1.3710 ; L726 LD, *** =1.0210 ; G755S LD, *** =6.2510 ; R780S LD, *** =8.6810 ; L781H LD, *** =1.4410 ; T787A LD, *** =1.3610 ; T787M LD, *** =1.2510 . In vitro ubiquitination assay was performed using UBE3A mutants expressed and purified from bacteria. Reactions were stopped at the indicated times and the formation of self-ubiquitinated UBE3A was monitored by western blot using an anti-UBE3A antibody. Representative images are shown from three independent experiments that produced similar results.",yes
PMC8713588,Figure_3,oa_package/4c/ad/PMC8713588.tar.gz,"['\n\nHaematoxylin eosin (H E) stain, 4 objective.']","Figure 3 Haematoxylin & eosin (H&E) stain, 4 objective. Histology shows a small, partly-crushed biopsy of ulcerated squamous-lined mucosa with parakeratosis and irregular squamous hyperplasia.",yes
PMC8679056,Figure_7,oa_package/75/b4/PMC8679056.tar.gz,"['\n16\n\nDiagnostic Findings, Part 2A bone resection is performed due to the absence of a nidus on imaging in this\npatient (s 7, 8, 9, and 10).', '.', '1177_23742895211060536-fig7"" position=""float""/>.', '1177_23742895211060536-fig10"" position=""float""/>Questions/Discussion Points, Part 2Describe the Gross and Histologic Findings From a Patient With Osteoid\nOsteoma as Depicted in s 7, 8, 9, and\n10A bone resection from a patient with osteoid osteoma is shown to demonstrate how\nan osteoid osteoma appears on gross exam.', '\n24,25\n\nWithin the section of cortical bone, there is a well-demarcated round to oval\nred-brown lesion (arrow) that represents the nidus surrounded by hemorrhage and\nsclerotic bone (s 7 and 8).', 'Authors Note:\ns 7, 8, 9, and 10 were obtained during the scope\nof US government employment for Dr Conran.']",Figure 7. The section of cortical bone shows a nidus represented by a well demarcatedred oval lesion surrounded by a hypervascular rim (arrow).,yes
PMC10759054,Figure_2,oa_package/56/d5/PMC10759054.tar.gz,['Plain CT images and diagrams of the neck to chest.'],Figure 2 Plain CT images and diagrams of the neck to chest. The images show multiple rib fractures and extensive traumatic haemopneumothorax.,yes
PMC8357321,Figure_3,oa_package/ab/4c/PMC8357321.tar.gz,['27Normal squamous epithelium as imaged by endomicroscopy (a) (d) along with coregistered histological sections (e) (h).'],Fig. 3 Normal squamous epithelium as imaged by endomicroscopy (a)(d)along with coregistered histological sections (e)(h). Scale bars measure . Dashed lines indicate the corresponding imaging depth as quantified in (a)(d). Image (c) and section (g)depict glycogen rich epithelium. Image (d)was captured .,yes
PMC6861366,Figure_1,oa_package/c0/c8/PMC6861366.tar.gz,"[', 2008; A).', 'Tau protein expression and tau phosphorylation in the hippocampus post-status epilepticus.', 'RT-qPCR revealed hippocampal tau mRNA levels to be relatively unchanged compared to control at all time-points analyzed post-status epilepticus (1 h 24 h; B).', '03; ANOVA post hoc Fisher s test; C].', '005; ANOVA post hoc Fisher s test] with this increase tapering off at 24 h post-status epilepticus (D).', 'Western blots using the phospho-tau antibody PHF-1 revealed no significant increase when normalized to total tau levels (E).']","Figure 1 Tau protein expression and tau phosphorylation in the hippocampus post-status epilepticus. Illustration of intra-amygdala injection of Kainic acid (KA) in mice for the induction of status epilepticus and chronic epilepsy. Flouro-jade B (FjB) staining shows neurodegeneration in the ipsilateral hippocampus at 24 h post-KA injection. Note: absence of FjB-positive cells at 4 h post status epilepticus ( = 4 per group). FjB-positive cells are mainly localized to the ipsilateral CA3 subfield of the hippocampus. Scale bar = 200 m. mRNA levels quantified by RT-qPCR showing no changes between control and post-status epilepticus ( = 4 per group). Representative Western blot ( = 1/lane) and corresponding graphs showing an increase in total tau (Tau-1; Ctrl vs. 4 h, = 0.03), AT8/Tau-1 (Ctrl vs. 4 h, = 0.03; Ctrl vs. 8 h, = 0.005) and PHF-1/Tau-1 protein levels in the ipsilateral hippocampus post-status epilepticus. GAPDH is shown as a loading control ( = 4 per group). One-way ANOVA with Fishers test. Data are presented as mean standard error of the mean (SEM). * < 0.05, ** < 0.01, *** < 0.001.",yes
PMC7543687,Figure_18,oa_package/d3/1b/PMC7543687.tar.gz,[],Figure18 A 73-year-old man was admitted with COVID-19. (a) CXR at presentation showed a left hilar mass concerning for lung cancer and bilateral peripheral consolidation typical for COVID-19. (b) Early follow-up staging CT confirmed a left upper lobe tumour and pulmonary emboli and (c) peripheral GGO with inter- and intralobular septal thickening in keeping with COVID-19. RIS codes to be applied in this case would be PCVCT2 + PCVCT4 + PCVPE1.,yes
PMC10546850,Figure_3,oa_package/04/56/PMC10546850.tar.gz,"['During the postoperative follow-up, the abscess was gradually reduced ().', 'At one-year postoperative follow-up, the patient had a relatively satisfactory outcome, with no sensory or motor deficits of the lower extremities ().', 'Repeated CT showed bony fusion of the implanted iliac bone and complete disappearance of the abscess, despite the presence of secondary lumbar kyphosis with spinal stenosis ().', '(a,b) A repeat CT examination two months after surgery showed a significant reduction of the abscess.']","Figure 3 (a,b) A repeat CT examination two months after surgery showed a significant reduction of the abscess. (c,d) Theabscess almost completely disappeared at five months postoperatively with no signs of recurrence. (e-g) One year after surgery,CT showed that bony fusion of the implanted iliac bone and disappearance of the abscess. (h,i) The patient obtained satisfactoryresult.CT: Computed tomography",yes
PMC4023008,Figure_5,oa_package/87/66/PMC4023008.tar.gz,['Ossification of the yellow ligament contributing to both central and bilateral lateral recess stenosis that was also confirmed on the preoperative MRILumbar Preoperative Parasagittal T2-Weighted MRI Study.'],Figure 5 Lumbar Preoperative Parasagittal T2-Weighted MRI Study. The preoperativeT2 MRI similarly documented multilevel stenosis from L2-S1. There was diffuse ventral intrusion from degenerative discal changes accompanied by dorsolateral ossification/hypertrophy of the yellow ligament; these findings contributed to marked lateral recess compromise. Also noted was the Grade I spondylolisthesis at the L45 level and Grade I-II spondylolisthesis at the L5S1 level,yes
PMC3195806,Figure_1,oa_package/d6/91/PMC3195806.tar.gz,"['A small diverticulum was also reported to be present, just above the aortic arch; see .', 'Report of two new cases and review of the literatureJournal of Cardiovascular Surgery198728440341116SmithJMReulGJWurashDCCooleyDARetro-oesophageal subclavian arteries: surgical management of symptomatic childrenCardiovascular Diseases1979633333417Pifarr RDieterRANiedballaRGDefinitive surgical treatment of the aberrant retroesophageal right subclavian artery in the adultJournal of Thoracic and Cardiovascular Surgery1971611154159554045818BaileyCPHiroseTAlbaJRe-establishment of the continuity of the anomalous right subclavian artery after operation for dysphagia lusoriaAngiology1965169509513582997919LemireGGRabbatAGTrudelJDysphagia lusoria: current surgical approachJournal of Cardiovascular Surgery19781931131365950420ShennibHDiethrichEBNovel approaches for the treatment of the aberrant right subclavian artery and its aneurysmsJournal of Vascular Surgery2008475106610701845564721KoppRWizgallIKreuzerESurgical and endovascular treatment of symptomatic aberrant right subclavian artery (arteria lusoria)Vascular2007152849117481369Barium swallow: the characteristic diagonal impression at the level of the third and fourth vertebrae.']",Figure 1 Barium swallow: the characteristic diagonal impression at the level of the third and fourth vertebrae.,yes
PMC8281787,Figure_6,oa_package/1b/5a/PMC8281787.tar.gz,[' FDG-18 PET MIP image showing intense radiotracer uptake in left parotid space mass (black arrow).'],"Figure 6 FDG-18 PET MIP image showing intense radiotracer uptake in left parotid space mass (black arrow). The additional sites of radiotracer uptake in left level II cervical lymph nodes (black circle) consistent with nodal involvement. The patient information at the time of injection: blood glucose -84 mg/dL, weight - 122 lbs, BMI - 22 kg/m , and time to injection - 64 minutes. FDG-18: fludeoxyglucose F-18; PET: positron emission tomography; MIP:maximum intensity projection",yes
PMC11412999,Figure_4,oa_package/ee/e5/PMC11412999.tar.gz,"[' 4A.', ' 4B).', ' 4C).', ' 4D and E), and mRNA expression (', ' 4F) were observed when compared to their control counterparts.', '\nThe study of histopathology and gene expression in animal tissues.', '\nAnimal microbiome studyAlpha diversity, measured using the Shannon index for control rats and urolithiasis rats after four weeks of FMT, showed an elevation in Shannon entropy for both groups.']","Fig. 4 The study of histopathology and gene expression in animal tissues. ( ) Histopathological examination of the kidney under a polarized light microscope, categorized into control rats (a, c, and e) and urolithiasis rats (b, d, and f). ( ) Analysis of mRNA expression in kidney tissue. ( ) Analysis of mRNA expression in jejunum tissue. ( ) Histopathological examination of the jejunum using immunohistochemistry against ZO-1 (ZO-1 stained orange or brown, cytoplasm stained in blue) ( ) Analysis of jejunal ZO-1 protein expression, and ( ) Analysis of jejunal mRNA expression. Statistical significance was denoted as * <0.05 compared to the control group.",yes
PMC7581659,Figure_2,oa_package/8d/bb/PMC7581659.tar.gz,"[' 2 displays median lesion SUVmax on PSMA versus FDG PET by cancer subtype.', '3, Additional file 1: C, D).', 'Box and whisker plot of Median SUVmax for all lesions by thyroid cancer subtype.', 'Diff = differentiatedPatients with metastatic thyroid cancer with greater PSMA uptake than FDG uptake: examples.']",Fig. 2 Box and whisker plot of Median SUVmax for all lesions by thyroid cancer subtype. Diff=differentiated,yes
PMC11518729,Figure_5,oa_package/9b/c0/PMC11518729.tar.gz,[],"Figure5 Gross specimen: A collection of gray-white, gray-yellow, and gray-brown tissue; Hematoxylin and Eosin staining microscopic examination: The tumor consists of morphologically diverse and large atypical cells, with large, deeply stained nuclei, prominent nucleoli, and occasional pathological mitotic figures and tumor giant cells; Immunohistochemical staining shows positive for vimentin; Ki-67 positive in 50% of cells (HE200).",yes
PMC7690166,Figure_4,oa_package/f5/29/PMC7690166.tar.gz,"[' 4).', 'H E stained slides through the gluteus medius bone tendon junction.', 'Samples demonstrate progressive degenerative changesTable 2Histology tendon and enthesis scores for lateral insertion of gluteus mediusTendon (SD)p-value (post hoc)aEnthesis (SD)P-value (post hoc)aComplete Tear8.']","Fig. 4 H&E stained slides through the gluteus medius bone tendon junction. Top images transmitted light, bottom images polarised light. Left - normal sample, middle - area adjacent to partial tear, right - area of complete tear. Samples demonstrate progressive degenerative changes",yes
PMC3335155,Figure_8,oa_package/4b/88/PMC3335155.tar.gz,"['Mouse nestin-positive blood vessels in the tumor were GFAP-negative (A, inset).', 'Large numbers of nestin-positive host cells were found at the immediate tumor border (C), whereas GFAP-positive host cells with typical astrocytic morphology were distributed loosely in a halo more distal to the zone containing mouse nestin-positive cells that surrounded the D566 human glioma implants (', 'Some GFAP-positive cells were adjacent to and showed arbors projecting onto blood vessels (B, inset).', 'In the GBM biopsy xenografts, similar peritumor accumulation of rat nestin positive cells was seen, with significant numbers of cells expressing both rat nestin and GFAP (D).', 'Double-stained cells in the tumor periphery were often elongated with several terminal processes (E); however, plump cells with astrocytic morphology were also seen, possibly representing reactive astrocytes (', 'Rat nestin and GFAP double-positive cells were more numerous in the tumor periphery, but were also found in the tumor core (G).', 'g008Localization of GFAP-expressing host cells and rodent nestin-positive cells in glioma xenografts.']",10.1371/journal.pone.0035150.g008,yes
PMC7813051,Figure_2,oa_package/9d/dc/PMC7813051.tar.gz,[],"FIGURE 2 A and B, showing irregular undermined ulcer with atrophic plaque on dorsum of left foot, (C and D) erythematous plaque with yellowbrown crust, (E and F) onycholysis and subungual hyperkeratosis, (G and H) 1wk after starting treatment, (I and J) 4wk after starting treatment and (MP) 9mo after stopping the treatment",yes
PMC8002116,Figure_1,oa_package/e0/5a/PMC8002116.tar.gz,"['However, vestibule, semicircular canals and cochlear on the right appeared hyperintense on FLAIR images () and on diffusion-weighted images, leading to the diagnosis of right labyrinthitis.', '018700600580102187486Right labyrinthitis: Hyperintensity (arrows) on axial (a) and frontal (b) FLAIR sections in right vestibule, semicircular canals and cochlear.']","Figure 1 Right labyrinthitis: Hyperintensity (arrows) on axial ( ) and frontal ( ) FLAIR sections in right vestibule, semicircular canals and cochlear.",yes
PMC4159772,Figure_7,oa_package/2c/6f/PMC4159772.tar.gz,"['Using this method, we are able to routinely visualize the Wdfy3 400 kDA isoform, but only in WT while in lysates of homozygous disc and lacZ mutants the 400 kDa isoform is always absent (Supplementary b, full size blots in Supplementary ', 'Using primers that span the transgenic gene disruption, we confirmed alternate read-through transcripts in lacZ allele mutants (Supplementary c).', 'Western blot analysis of these lysates showed no significant differences between genotypes when probed with antibodies against LC3 or P62 (Student s t-test, n=3 for either genotype and stage; a, b).', '5 WT and disc/disc embryos showed no significant differences in size or density of P62+ puncta (autophagosomes) between the genotypes (Student s t-test, n=3 for either genotype; c, d).', '5 WT and disc/disc forebrains revealed no significant changes in the total amount of mono- and polyubiquitinated conjugates (Student s t-test, n=3 for either genotype; e, f).', 'No changes in macroautophagic flux of disc/disc embryos(a) Western blot analysis of LC3 and P62 on lysates of WT and disc/disc mutant brains at developmental stages E12.']","Figure 7 No changes in macroautophagic flux of embryos ( ) Western blot analysis of LC3 and P62 on lysates of WT and mutant brains at developmental stages E12.5 and E15.5 demonstrate no changes in expression levels between the genotypes. ( ) Quantification of LC3II/I ratio and P62 levels confirm equal expression levels in the two genotypes. ( ) Immunofluorescent analysis of P62 puncta in primary neuronal cultures from WT and embryos show no significant differences between the genotypes as quantification results in ( ) demonstrate. ( ) Western blot analysis of ubiquitinated proteins shows no differences between the genotypes as the quantification of results in ( ) demonstrates. All analysis by Students -test. Number (n) of samples of either genotype shown in the bar diagrams, which display mean and SEM. Scale bar 20 m.",yes
PMC2890657,Figure_2,oa_package/6a/32/PMC2890657.tar.gz,['Macroscopic aspect of liver and functional hepatic tests.'],"Figure 2 . A, a) a representative image a normal rat liver, b and c shows a recovered hepatic smooth texture in animals treated with the combinatorial gene therapy at 8 and 10 days, respectively, as compared with irrelevant gene therapy (e and f), d) shows a control fibrotic liver injected with saline. Histograms in B, show a significative tendency to normal values of hepatic functional tests (ALT and AST).",yes
PMC2851648,Figure_4,oa_package/85/9c/PMC2851648.tar.gz,"['N-SYN induces DAergic cell apoptosis in a dose-dependent mannerSHSY-5Y cells exposed to SYN and N-SYN were TUNEL labeled to assess cellular apoptosis ().', 'g004N-SYN induces apoptosis of SHSY-5Y cells.']",10.1371/journal.pone.0009956.g004,yes
PMC10419098,Figure_7,oa_package/be/81/PMC10419098.tar.gz,"['ChatGPT-4 also provided answers for all of those questions ().', 'A response from ChatGPT-4 for the question Provide answers to those questions [15].']",Figure 7 A response from ChatGPT-4 for the question Provide answers to those questions [ ].,yes
PMC10613125,Figure_5,oa_package/fa/37/PMC10613125.tar.gz,[],"FIGURE 5 Palmitate and glucose increase EV APP secretion in vitro from cells. (a) Differentiated human HK532 cortical stem cells were treated with increasing palmitate or oleate concentrations (0 [BSA vehicle], 50, 75, 100 and 150M) for 48h. EVs were purified from culture supernatant and analysed by WB for APP and flotillin. (b) Relative levels from WB analysis for APP versus flotillin in 0 or 150 M palmitate or oleate represented in the graph. (c) HK532 cells were treated with BSA (control) or 150M palmitate for 48h. EVs were purified from culture supernatant and analysed by WB for CTF (Cterminal fragment). (d) Relative levels from WB analysis for CTF versus TSG101 is represented in the graph. (e) eCNs were treated with control (media) or glucose (glu; 50mM) for 24h. EVs were purified from cell culture media by ultracentrifugation and analysed by WB for APP versus flotillin and TSG101 as EV markers. (f) Relative levels from WB analysis for EV APP versus flotillin are represented in the graph. Data are presented as meanSEM from at least three separate experiments. * <0.05, ** <0.01, *** <0.001, by Student's test.",yes
PMC9432091,Figure_10,oa_package/7a/d4/PMC9432091.tar.gz,[],"Fig. 10 Out car traffic accidenct in an 83-year-old woman. CT scan shows the superior gluteal artery (white arrows) arising from the posterior division of the IIA. The superior gluteal artery exits the pelvis through the greater sciatic foramen (dotted line), above the P. The superficial branch (arrowhead) runs between the G max and the G med muscles. The deep branch (empty arrowhead) runs between the G med and the G min muscles A focal, highly attenuated area (black boxs) in the arterial phase increased in size in the delayed phase, mainly in the G max muscle. Injury to the superior gluteal artery was considered as the cause of the active bleeding in the left buttock area. Another active bleeding (asterisks) was mentioned in . An angiogram demonstrates active bleeding (black box) from the superior gluteal artery (white arrow). The superior gluteal artery passes the superior portion of the greater sciatic foramen, while the inferior gluteal artery (black arrow) passes the inferior portion of the same foramen. Therefore, the greater sciatic foramen would be located at the dotted circle area. The lateral sacral artery is also visualized (circle). EIA = external iliac artery, G max = gluteus maximus, G med = gluteus medius, G min = gluteus minimus, IIA = internal iliac artery, P = piriformis muscle",yes
PMC4939890,Figure_3,oa_package/5f/1a/PMC4939890.tar.gz,['Signaling between cardiac neural crest cells (CNCCs) and the second heart field (SHF) is necessary for proper development of the aortic valve.'],Figure 3 Signaling between cardiac neural crest cells (CNCCs) and the second heart field (SHF) is necessary for proper development of the aortic valve. Crosstalk between neural crest cells and the SHF ensures the production of fibroblast growth factor 8 (Fgf8) in a Notchdependent manner. This step is essential as it contributes to the production of BMP24 and allows tissue reorganisation and loss of cellular components through apoptosis. Disruption of Notch signaling in the SHF leads to defective neural crest cell patterning and the formation of leaflets with a bicuspidlike morphology in mice.,yes
PMC8648445,Figure_10,oa_package/48/62/PMC8648445.tar.gz,[],"Figure 10 Comparison of conventional MRI, optimized MRI, and pathological results after NCRT. (a) Conventional MRI image diagnosed as TRG2; (b) optimized MRI image diagnosed as TRG2; and (c) pathological results diagnosed as TRG3.",yes
PMC11508230,Figure_7,oa_package/41/1f/PMC11508230.tar.gz,"['On T2-weighted images or short tau inversion recovery (STIR) sequences, VCFs typically show hyperintense signals, reflecting edema or hemorrhage within the vertebral body ().', 'In these sagittal MRIs, the arrows show acute to subacute burst compression fracture of the superior endplate of L1 with accompanying T1 hypointense (top left)/STIR hyperintense (top right) marrow signal abnormality.']","Figure 7 In these sagittal MRIs, the arrows show acute to subacute burst compression fracture of the superior endplate of L1 with accompanying T1 hypointense ( )/STIR hyperintense ( ) marrow signal abnormality. In the axial T2 at this level ( ), the arrow shows 0.6 cm retropulsion of the superior endplate contributing to moderate spinal canal stenosis at this level, with crowding of the cauda equina nerve roots. In the axial post-contrast T1 ( ), the arrow shows heterogeneous enhancement which is likely reactive and expected.",yes
PMC4356726,Figure_2,oa_package/35/99/PMC4356726.tar.gz,[],Fig. (2) Radiographic appearance of an implanted device.,yes
PMC2773124,Figure_4,oa_package/01/66/PMC2773124.tar.gz,"[' 4a).', '', 'Approximate molecular masses are in kilodaltons\nAlthough clearly distinguishable from a type 1 and 2 concurrence, also the PrPSc type 2 profile in VV subjects was sometimes characterized by a doublet comprising a ~18.', ' 4b).']","Fig.4 Western blot profiles of PrP (3F4 antibody) from different brain regions of the sCJD VV 2+1 subject with the more significant type 1 deposition. Areas shown include the middle frontal gyrus ( ), middle temporal gyrus ( ), parietal cortex ( ), lateral occipital cortex ( ), insular cortex ( ), putamen nucleus ( ), medial thalamic nuclei ( ), and cerebellum ( ). Approximate molecular masses are in kilodaltons. Western blot profiles of PrP doublets in sCJD VV2, sCJD MM 1+2 and sCJD MV 2K. and show the association of a ~18.5kDa fragment with the typical 19kDa type 2 band in sCJD VV2 cerebellum ( , =vermis, =hemisphere). Approximate molecular masses are in kilodaltons",yes
PMC11276385,Figure_2,oa_package/4c/17/PMC11276385.tar.gz,"['It is associated with a range of pediatric skeletal conditions () including Slipped Capital Femoral Epiphysis, Blount s Disease (Genu Varum, or bowed legs) and Genu Valgum (knock knees).', 'The possible clinical consequences of childhood obesity.']",Figure 2 The possible clinical consequences of childhood obesity. The figure shows the possible clinical consequences of obesity in children. ( ) Slipped capital femoral epiphysis in which the epiphysis of the femur separates from the metaphysis at the growth plate; ( ) Genu Varum (bowed legs); and ( ) Genu Valgum (knocked knees).,yes
PMC3857900,Figure_2,oa_package/67/65/PMC3857900.tar.gz,"['Anorectal, transrectal, and transvaginal ultrasonography can help to identify a fistulous tract, as well as its relation to the adjacent anatomical structures (s 2(a), 2(b), and 2(c)) [34, 35].', '001""/>Rectovesical fistula: anorectal endosonographic view of a fistulous orifice in the urinary bladder (arrows) (a), transrectal ultrasonographic view of a fistulous orifice (arrows) located 6 mm from the internal outlet of the bladder (crosses) (b), and transrectal ultrasonographic view of a fistulous tract adjacent to the left lobe of the prostate (arrows) (c).']","Figure 2 Rectovesical fistula: anorectal endosonographic view of a fistulous orifice in the urinary bladder (arrows) (a), transrectal ultrasonographic view of a fistulous orifice (arrows) located 6mm from the internal outlet of the bladder (crosses) (b), and transrectal ultrasonographic view of a fistulous tract adjacent to the left lobe of the prostate (arrows) (c).",yes
PMC9441100,Figure_3,oa_package/cd/d4/PMC9441100.tar.gz,"[' 3a b, arrows).', ' 3b, arrowheads).', 'TDP-43 and pTau are co-expressed in necroptosis-positive neurons.', 'We exclude the signal observed being considered as unspecific lipofuscin because such signal usually shows in the blue (Hoeschst) channel as orange granules, which in this case is absentNext, we aimed at investigating the impact of LATE-NC on pMLKL-positive GVD severity, neuronal death, and pTau pathology.']","Fig. 3 TDP-43 and pTau are co-expressed in necroptosis-positive neurons. Triple labeling immunofluorescence with antibodies against pTDP-43 (S409/S410), pTau (S202/T205) and pMLKL (S358) in the CA1 sub-hippocampal field of an AD case. Nuclei are stained with Hoechst solution. Of note, pTDP-43 and pMLKL are co-localized in some GVD granules (arrows) and pTDP-43 and pTau co-localize in nearby neurons (arrowheads). We exclude the signal observed being considered as unspecific lipofuscin because such signal usually shows in the blue (Hoeschst) channel as orange granules, which in this case is absent",yes
PMC5004616,Figure_3,oa_package/16/02/PMC5004616.tar.gz,['.'],"Fig. 3. - (A-C) Morphological characteristics of ethanol-treated and untreated larvae at 6dpf. (D-I) H&E staining of transverse sections at site of abdomen in untreated and ethanol-treated larvae. Boxed areas in D,F,H magnified in E,G,I. (J,L,N) Reconstructed images of vascular structure in untreated and ethanol-treated larvae by confocal imaging. (K,M,O) Tomography of vascular structure in control and alcohol-treated larvae by confocal imaging. Asterisk in N,O indicates destruction in dorsal aorta.",yes
PMC7468111,Figure_2,oa_package/b5/89/PMC7468111.tar.gz,"[' 2B).', ' 2A,C).', ' 2C).', 'Alpha-synuclein protein expression is increased in LRRK2 mutant astrocytes.', 'PD astrocytes manifest a reactive phenotype with increased cytokine secretion after inflammatory stimulationTo assess the effect of inflammatory stimulation on secretion of cytokines from astrocytes, we first exposed the cells to TNF , IL-1 and IFN .']","Figure 2 Alpha-synuclein protein expression is increased in LRRK2 mutant astrocytes. ( ) Representative immunofluorescent images of astrocytes stained for -synuclein and S100B. Nuclei stained with DAPI. Scale bar 20m. ( ) Relative gene expression level of gene in astrocytes over 4-month period shown as fold change to healthy cells. The results are normalized to beta-actin expression. ( ) Protein expression in astrocytes and the released amount of -synuclein to media measured with ELISA. Data is shown as a fold change to control (healthy) and results are normalized to total protein content. Four independent experiments. Bars represents meanSD, **p<0.01, ***p<0.001.",yes
PMC10405242,Figure_9,oa_package/7d/b6/PMC10405242.tar.gz,[],"FIGURE 9 AP chest Xray with representation of chamber anatomy. The ventricular lead (b) is seen coursing along the right lateral aspect of the heart, before eventually bowing at the apex and lodging in the RV. The atrial lead (a) is projected over the right atrium (RA).",yes
PMC10342058,Figure_1,oa_package/ee/f3/PMC10342058.tar.gz,"['For PM, DM, NM and dystrophies, no increase of NLRP3 was noted (A).', 'The mRNA level of NLRC5, a key activator of MHC class I transcription [18], was significantly overexpressed in IBM samples compared to controls (B).', 'Moreover, an increased mRNA expression of NLRC5 in IBM muscle compared to the control could be observed (B).', '00227117419Expression analysis in muscle biopsy material from patients with inclusion body myositis shows an increased expression of NLRP3 and NLRC5.']","Figure 1 Expression analysis in muscle biopsy material from patients with inclusion body myositis shows an increased expression of NLRP3 and NLRC5. Quantification of the mRNA expression level of NLRP3 ( ) and NLRC5 ( ) relative to the endogenous control GAPDH in muscle biopsy material from healthy controls (n = 11), inclusion body myositis (IBM, n = 14), dermatomyositis (DM, n = 10), polymyositis (PM, n = 13), muscular dystrophy (Dys, n = 10), and necrotizing myopathy (NM, n = 10) patients. (ANOVA, levels of significance indicated by * = 0.0492, *** = 0.0006).",yes
PMC10627428,Figure_3,oa_package/78/20/PMC10627428.tar.gz,['Laparoscopic images demonstrating (A) the application of an ENDOLOOP Ligature to the pedicle of the torted right paratubal cyst and isolated fallopian tube and (B) the normal-appearing appendix.'],Figure 3 Laparoscopic images demonstrating (A) the application of an ENDOLOOP Ligature to the pedicle of the torted right paratubal cyst and isolated fallopian tube and (B) the normal-appearing appendix.,yes
PMC6894201,Figure_1,oa_package/dd/15/PMC6894201.tar.gz,"[' 1, 2, 3, and 4.', '0001HR MRI High-resolution magnetic resonance imaging\nA 68-year-old woman with esophagogastric junction cancer was pathologically diagnosed using HR MRI as having stage T1b, Sierwert II.', 'f The lesion and mucosal tissue had similar degrees of enhancement on the delayed phase of the T1W image\nA 48-year-old man with esophagogastric junction cancer was pathologically diagnosed using HR MRI as having stage T4b, Sierwert II.']","Fig. 1 A 68-year-old woman with esophagogastric junction cancer was pathologically diagnosed using HR MRI as having stage T1b, Sierwert II. The axial T2W image shows partial thickening of the cardia wall with a decreased signal (arrow). The axial diffusion-weighted image with b=800s/mm shows restricted diffusion in the cardia. The lesion was not clearly shown on the axial T1W image. Axial and arterial phases of a contrast-enhanced image show significant enhancement of the lesion (arrow). Persistent enhancement of the lesion was observed on the enhanced and venous phases of T1W images. The lesion and mucosal tissue had similar degrees of enhancement on the delayed phase of the T1W image",yes
PMC4873509,Figure_1,oa_package/35/72/PMC4873509.tar.gz,[],"FIGURE 1 The virtual electrodes are shown as blue (anterior), red (center), and green (posterior) dots on sagittal, coronal and axial brain slices (left, middle, and right panel, respectively).",yes
PMC5271427,Figure_2,oa_package/d1/77/PMC5271427.tar.gz,['Representative images of A PP immunolabeling in the brain and retina of 11-month-old WT and Tg mice.'],"Fig.2 Representative images of APP immunolabeling in the brain and retina of 11-month-old WT and Tg mice. In WT parietal cortex (A) and hippocampus (B), APP localizes to the cytoplasm of neurons. In Tg mice, neuronal expression of APP is higher, and numerous dystrophic neurites surrounding amyloid plaques are present within the parietal cortex (C, arrowheads) and hippocampus (D). In the WT retina, APP localizes to the cytoplasm of RGCs (arrow), but is barely detectable within amacrine cells (arrowhead) or other neuronal cell types. In Tg mice, APP expression is markedly higher in RGCs (arrow), and is also clearly detectable in other neuronal populations (arrowhead); however, no APP-positive dystrophic neurites are evident. Scale bar: A, C=100 m; E, G=50 m, B,D,F,H=25 m.",yes
PMC6227800,Figure_1,oa_package/d2/c5/PMC6227800.tar.gz,"['Representative 11 C-PBB3 SUVR images of the AD patients with low and high AS scores were shown in panels 1A and 1B of figure 1, respectively.', 'In line with ROI-based analyses, voxel-by-voxel comparisons of PBB3 SUVR images between the two patient groups by statistical parametric mapping revealed that AD patients with high AS scores presented significantly greater radioligand retention in OFC than patients with low AS scores (figure 1C).', 'Representative11 C-PBB3 SUVR PET images of AD patients with low (A) and high (B) AS scores.', '05 (online supplementary figure 1).']","Figure 1 Representative C-PBB3 SUVR PET images of AD patients with low (A) and high (B) AS scores. High radioligand retention in the superior sagittal sinus (yellow triangles in panels a and b) is a non-specific radioactivity accumulation. AD patient with high AS score showed remarkably increased radioligand retention in the vicinity of OFC (yellow arrows in panel b) relative to the patient with low AS score (A). Statistical parametric map illustrates greater accumulations of PBB3-detectable tau lesions in OFC in AD patients with high AS scores relative to those with low AS scores (C). Data are thresholded at false discovery rate-corrected p value <0.05and extent threshold >600 voxels (C). C-PBB3, C-pyridinyl-butadienyl-benzothiazole 3; AD, Alzheimers disease; AS, Apathy Scale; OFC, orbitofrontal cortex; PET, positron emission tomography; SUVR, standardised uptake value ratio.",yes
PMC5630055,Figure_2,oa_package/ec/25/PMC5630055.tar.gz,"['MRI of pelvis showed two hyper-intense foci (T2 STIR), one intramedullary and one in the subcutaneous plane with diffuse enhancement on contrast suggestive of phlegmonous foci ().']",Fig. 2: MRI-2 hyper-intense foci; intramedullary and in subcutaneous plane with enhancement 99mTc-MDP scan increased tracer uptake in the right posterior ilium.,yes
PMC8889165,Figure_5,oa_package/d7/0d/PMC8889165.tar.gz,"[' presents these histopathological findings.', 'Histological sections of skin biopsy showing subcorneal pustulation and splitting.']","Figure 5 Histological sections of skin biopsy showing subcorneal pustulation and splitting. Epidermal spongiosis, perivascular inflammation, papillary dermal oedema and neutrophilic, eosinophilic and lymphocytic cell infiltrate. No evidence of epidermal necrosis. (H&E stain).",yes
PMC3501973,Figure_9,oa_package/0f/20/PMC3501973.tar.gz,"['5%) did not result in a significant improvement (A).', '4 s) (B).', 'Behavioural analysis of mice treated with the rho kinase inhibitor fasudil for 6 weeks after the initial MPTP application.']","Figure 9 Behavioural analysis of mice treated with the rho kinase inhibitor fasudil for 6 weeks after the initial MPTP application. ( ) Quantification of the percentage of rears against the wall with both paws, only right paw, only left paw or free rears. ( ) Quantification of the average running time out of three runs on an accelerating rotarod. Bars represent means SEM. * < 0.05, ** < 0.01, *** < 0.001. n.s = not significant.",yes
PMC6759112,Figure_4,oa_package/c8/00/PMC6759112.tar.gz,['Cancer-specific survival of small tumor with high-level thrombus group (Group A) and large tumor with low-level thrombus group (Group B).'],Figure 4 Cancer-specific survival of small tumor with high-level thrombus group (Group A) and large tumor with low-level thrombus group (Group B).,yes
PMC9521189,Figure_1,oa_package/c4/58/PMC9521189.tar.gz,"['As illustrated in , phosphorylation of I B by a kinase protein called I B kinas (Ikk), results in the separation of I B from NF- B.']","FIGURE 1 Activation process of NF-B transcription factor through an AGE-RAGE signaling pathway. Activation of RAGE through AGE interaction transduces a signal for the phosphorylation of IkB by IKK. Phosphorylated IkB then could be detached from the cytosolic NF-kB transcription factor. Multiple genes such as cytokines, chemokines, and adhesion molecules are activated when active and free NF-kB translocate into the nucleus. These proteins trigger oxidative stress, inflammation, and cellular damage, all of which contribute to diabetic complications.",yes
PMC7654323,Figure_5,oa_package/0f/05/PMC7654323.tar.gz,"['As shown in the UMAP representation ( 5\nA), untreated and baricitinib-treated RMs had different BAL cellular distribution starting from 4 days after infection, corresponding with the time point of peak inflammation and viremia, including in neutrophils.', 'Analyses of BALs showed an early recruitment of neutrophils in the lung at 4 days after infection during the peak of viremia, particularly in the untreated RMs, which all maintained higher frequencies of neutrophils at later stages of infection (10 11 days after infection) than did baricitinib-treated RMs ( 5B; p = 0.', 'In blood, neutrophils ( 5C) remained relatively stable after infection as compared to before infection and at lower levels in untreated as compared to treated animals at the latest experimental points (p = 0.', 'The levels of CD14+CD16 ( 5D) and CD14+CD16+ monocytes in the BALs were, on average, slightly higher in untreated RMs at 4, 7, and 10 days after infection, with the difference due to 3 of 4 untreated RMs having levels higher than the treated animals at specific time points ( 5D).', 'Given that the flow-cytometry data of BALs show a reduced migration of neutrophils to lung in baricitinib-treated RMs, we next measured neutrophil extracellular trap (NET) activity by quantification of extracellular DNA via Sytox staining, a functional readout of NETosis activity (s 5E and 5F) and by quantification of citrullinated H3 ( 5G), a systemic marker indicating a post-translational modification thought to precede DNA decondensation during NETosis.', 'Baricitinib-treated RMs showed decreased NET formation by blood neutrophils at 4 (more evident for citrullinated H3, 5G; p = 0.', '0571) and 10 (more evident for Sytox staining, 5F; p = 0.', 'Finally, when the formation of NETs was examined directly in the lung by IHC staining for citrullinated H3, 3 out of 4 untreated RMs showed presence of NETs, whereas NETs were virtually absent in treated RMs ( 5H).', ' 5Baricitinib-treated RMs have decreased infiltration of innate immune cells and lowered neutrophil NETosis(A) UMAP analysis of BALs in baricitinib-treated (n = 4) and untreated (n = 4) SARS-CoV-2-infected RMs before infection (D 5 PI; baseline), and at 4 and 10 days after infection.', ' S6Flow cytometry gating strategy for innate and adaptive cells, related to s 5 and 6Representative gating strategy of (A) neutrophils, (B) neutrophil infiltration in BAL at baseline, and 4 and 10 days after infection, and (C) T cell populations analyzed in the study.']","Figure2 Reduced respiratory disease and lower levels of lung pathology in baricitinib-treated RMs (A) Representative ventrodorsal radiograph of an untreated RM before SARS-CoV-2 infection (5days before infection), and at 4, and 7days after infection. Red squares indicate regions of pulmonary infiltrates and opacity. (B and C) Daily (B) and cumulative (C) radiograph scores; ventrodorsal and lateral radiographs were scored for the presence of pulmonary infiltration by a clinical radiologist according to a standard scoring system (0: normal; 1: mild interstitial pulmonary infiltrates; 2: moderate pulmonary infiltrates with partial cardiac border effacement and small areas of pulmonary consolidation; 3: severe interstitial infiltrates, large areas of pulmonary consolidation, alveolar patterns, and air bronchograms). (D and E) Fold change to 2days after infection for ferritin (D) and C-reactive protein (CRP) levels (E). (F and G) Panel (F) shows 100 magnification, and (G) shows 200 magnification (zoomed in from F), representative lung lesions in an untreated SARS-Cov-2-infected RM with focally extensive interstitial pneumonia, type 2 pneumocytes hyperplasia, alveolar septal thickening, syncytia formation (arrow), neutrophils, and macrophages infiltrations (arrowhead). (H) 200 magnification, Thyroid Transcription Factor-1 (TTF-1) staining with prominent type 2 pneumocyte hyperplasia (brown) in a control SARS-CoV-2-infected RM. (I and J) Panel (I) shows 100 magnification, and (J) shows 200 magnification (zoomed in from I), treatment effects of baricitinib in SARS-CoV-2-infected RMs with a reduction in pulmonary lesions, lesser inflammatory infiltrates (arrowhead), and reduced type 2 pneumocyte hyperplasia. (K) 200 magnification, TTF-1 staining with lesser type 2 pneumocyte hyperplasia (brown) after baricitinib treatment. (L) Average pathology score per lobe. (M) Total pathology score. (N) Pathology scores for individual parameters. Scale bar, (F) and (I): 100M; (G), (H), (J), and (K): 50M. Bars in (D), (E), (L), (M), and (N) indicate mean values for baricitinib-treated (blue) and untreated (red) SARS-CoV-2-infected RMs. Each symbol represents individual animals. Statistical analysis in (D), (E), and (L)(N) were performed using non-parametric Mann-Whitney test. Statistical analyses were performed two-sided with p0.05 deemed significant. Ranges of significance were graphically annotated as follows: p< 0.05. See also BS2I.",yes
PMC11535861,Figure_2,oa_package/5a/cb/PMC11535861.tar.gz,['miRNAs involved in cardiac hypertrophy through cardiomyocyte size and fibrosis.'],Figure 2 miRNAs involved in cardiac hypertrophy through cardiomyocyte size and fibrosis.,yes
PMC10720104,Figure_2,oa_package/6c/70/PMC10720104.tar.gz,"['2A).', '2B).', '2C).', 'In CIA FLS, WFR significantly reduced abnormally elevated MMP3 and fibronectin mRNA levels (D).', '2E), and WB detection showed that WFR decreased the protein expression of fibronectin in CIA FLS (p 0.', '2F).', '2G) (p 0.', '01)WFR inhibits the pathological-related genes and the FLS proliferation.', '01Wnt11 significantly higher expression in RAWnt11 has an important promoting effect on cardiac tissue proliferation and tumor cell proliferation [15 19].', 'F Fibronectin (abcam: ab268020).']","Fig. 2 WFR inhibits the pathological-related genes and the FLS proliferation. ELISA showed that WFR effectively reduced TNF-, IL-6, and IL-1 in serum of CIA rats ( ). WFR effectively decreased the expression of MMP3 and fibronectin mRNA in the synovium of CIA rats ( ). The cell dose of WFR was selected according to CCK8 experiment, and WFR significantly inhibited the proliferation of CIA FLS when the dose concentration was 200 nM ( ). WFR effectively decreased the expression of MMP3 and fibronectin mRNA in CIA FLS ( ). Immunofluorescence detection showed that WFR decreased the protein expression of MMP3 in CIA FLS ( ) (scale bar = 50 m). WB detection showed that WFR decreased the protein expression of fibronectin in CIA FLS ( ). CCK-8 detection showed that WFR significantly inhibited the proliferation of CIA FLS ( ). *CIA group vs normal, #CIA group+WFR vs CIA group, = 3. * < 0.01",yes
PMC3720593,Figure_3,oa_package/97/8b/PMC3720593.tar.gz,"['mimicus, can also similarly hydrolyze fibronectin (, Panel A, lane 2).', 'When probed with the scFv Fn52 in a western blot, following SDS-PAGE under reduced conditions, binding of the scFv is seen to occur only with the lower molecular weight fragments of fibronectin, both in the case of whole fibronectin molecule (A, lane 6), as well as collagenase-treated fibronectin (lane 7).', 'Fn52 does not show any binding to the intact molecule of fibronectin (A, lane 6), although the transfer of the protein to the membrane was confirmed by amido black staining of the membrane (A, lanes 4 and 5).', 'g003Fn52 binds to cryptic regions in the fibronectin.', 'g003""/>A western blot of the 70 kDa and 30 kDa fragments of fibronectin sourced commercially (, Panel B, lanes 3 and 4 respectively), using the scFv Fn52, also showed binding of the scFv to the 70 kDa and the 30 kDa fragments independently.', 'No binding of Fn52 was observed with the intact molecule of fibronectin (B, lane 2); however, anti-fibronectin antibody (Sigma Chemicals), was observed to bind to the intact fibronectin (B, lane 1).']",10.1371/journal.pone.0069343.g003,yes
PMC7516190,Figure_2,oa_package/21/37/PMC7516190.tar.gz,"['Robust skipping of exon 23, exons 56+57, exons 58+59, and exon 70 was detected by single round RT-PCR (exon 23) or nested RT-PCR, as shown in 2.', ' 2Analysis of Dystrophin Isoforms Induced by PPMO-Mediated Exon Skipping In VitroSingle-round and nested RT-PCR analysis of DMD transcripts confirming robust skipping of exon 23, exons 56+57, 58+59, and exon 70 in vitro.', 'The skipping of exons 56+57 in C57 mice and the exclusion of exons 23+56+57 in mdx mice was not as efficient as in the in vitro experiments ( 2D).']","Figure1 Dystrophin Exon Map, Downstream Exon 55, and the Dystrophin Glycoprotein Complex (A) The majority of exons downstream of dystrophin exon 55 are out-of-frame. These exons encode important domains for the assembly of the dystrophin glycoprotein complex. SLR, spectrin-like repeats. (B) The distal third of dystrophin interacts, either directly or indirectly with proteins in the dystrophin glycoprotein complex, including / dystroglycans, //// sarcroglycans, / syntrophins, and / dystrobrevins to stabilize the sarcolemma. ABD, actin binding domain; H, hinge; DYG, dystroglycan; SYB, syntrophin binding site; CC1 and CC2, coiled coil 1 and 2 (image not to scale).",yes
PMC6607360,Figure_4,oa_package/e2/1c/PMC6607360.tar.gz,"['In pure ATL, the cerebral cortex overlying the PVWM injury remains normal signal intensity on FLAIR, while in patients with purely HIE the overlying cortex is mildly hyperintense [2] ().', 'A 19 year old male with oral methadone overdose-related ATL presented with acute left sided weakness and upper motor neuron signs.', '4.']","Fig. 4 A 19year old male with oral methadone overdose-related ATL presented with acute left sided weakness and upper motor neuron signs. : The reduced diffusion bilaterally involves the PVWM ( ) on DWI MRI ( ) and ADC map ( ), with bilateral symmetric periventricular hyperintensity on FLAIR ( ). : There is also reduced diffusion within the optic radiations on DWI MRI ( ) and ADC map ( ), also having abnormal signal on FLAIR ( ).",yes
PMC9263923,Figure_4,oa_package/8d/d4/PMC9263923.tar.gz,"['05, B).', '05, C), whereas no obvious hippocampal neuron loss was observed in the PCF group and ABR-215757 group.', 'In this study, the expression level of BDNF was downregulated in the AMI group compared with the sham group and showed an upward trend after administration of PCF and ABR-215757, but there was no statistical significance between groups (D).']","FIGURE 4 The effect of PCF on the neurotransmitter disorder and decreased neurogenesis caused by myocardial infarction. HPLC analysis of 5-HT in the hippocampus of rats ( = 3). Ratio of kynurenine to tryptophan. Nissls staining in the dentate gyrus region of hippocampus from different groups. Western blotting detected the protein expression levels of BDNF in the hippocampus ( = 3). 5-HT: 5-hydroxytryptamine; Kyn: kynurenine; Try: tryptophan; BDNF: brain-derived neurotrophic factor; DG: dentate gyrus. * < 0.05, ** < 0.01, compared with the sham group. # < 0.05, compared with the AMI group. & < 0.05, compared with the AMI group.",yes
PMC4754353,Figure_12,oa_package/39/a7/PMC4754353.tar.gz,[],"Figure 2 GlyRS specifically binds Nrp1 and antagonizes VEGF-Nrp1 interaction , pull-down (PD) of GlyRS proteins by the ectodomain of Nrp1 but not TrkB. , Co-immunoprecipitation (IP) to detect GlyRS-Nrp1 interaction in neural tissues of wild-type (WT) and P234KY- mice (CMT). , Co-immunoprecipitation (IP) to detect GlyRS-Nrp1 interaction in lymphocytes from CMT2D patients carrying the L129P mutation (n=5) and from healthy individuals (n=3). , Domain mapping using IP identifies the b1 domain of Nrp1 as the main binding site of GlyRS . , PD assay showing the competition between P234KY-GlyRS and VEGF-A proteins for Nrp1 (b domains) binding. , Schematic of facial motor neuron migration ( ) and facial motor nucleus ( ) in open-book preparations of WT (left half) and VEGF/Nrp1-deficient mouse hindbrains at E13.5 (right half) . , Fluorescence labeling of facial motor neuron somata and axons by ISL :GFP-F on one side of E13.5 mouse hindbrain of open-book preparation. , Immunostaining of Isl-positive facial nucleus on one side of the E13.5 mouse hindbrain of open-book preparation. Scale bar represents 200m in .",yes
PMC4811858,Figure_9,oa_package/7c/6c/PMC4811858.tar.gz,"['In one case of mucinous cystic neoplasm, microscopic evaluation revealed an associated invasive carcinoma, which was missed both on macroscopic evaluation and on imaging, even retrospectively (a and b).', ')']","Fig. 9 A mucinous cystic neoplasm is shown, presenting a fibrotic area (blue arrow) and no evidence of adenocarcinoma (a) in the macroscopic evaluation. Microscopically (b: H/EX200) an associated invasive carcinoma of less than 0.5cm in size behind the fibrotic area was revealed (yellow arrow). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)",yes
PMC4157958,Figure_2,oa_package/25/27/PMC4157958.tar.gz,"['The papillary dermis showed minimal to focally mild perivascular infiltration of lymphocytes and neutrophils (A).', 'Histological features of clinically healthy, ID and footrot samples.', 'Perivascular immune cell infiltration was seen surrounding dilated superficial dermal blood vessels and resulted in a cell dense dermal epidermal junction, predominantly involving lymphocytes, neutrophils and a few plasma cells (B).', 'Migrating leukocytes in the stratum basale and stratum spinosum represented cells that had moved through the dermal epidermal junction (exocytosis) (B), with neutrophilic degranulation observed in the superficial epidermis.', 'The dermis showed large numbers of perivascular lymphocytes, neutrophils and fewer plasma cells leading to a cell dense migration through the papillary dermis and epidermis (C).', 'Purulence, composed of large numbers of non-viable neutrophils, necrotic debris and plasma proteins, was seen frequently in areas of epidermal degeneration and necrosis and in areas of epidermal dermal clefts (D).', 'Epidermal ballooning, characteristic of hydropic cell degeneration, was noted with marked swelling of the cytoplasm and additional condensation (pyknosis) of the nucleus (D).', 'Increased fibrous tissue proliferation (superficial dermal scarring) occurred in some cases indicating a chronic reaction due to the loss of tissue integrity at the skin hoof interface (E).', 'The papillary dermis showed vascular congestion and dilated lymphatic vessels with dermal oedema (C), as a consequence of the intense epidermal and dermal inflammation.']","Supplementary Fig. S1 TLR2, TLR4 and IL-1 transcript levels for healthy, ID and footrot (FR) samples, pooled by foot disease state for all samples and by foot disease state for each animal disease group (healthy, ID, FR), whereby clinically healthy animals only had healthy feet, ID animals had healthy and ID feet and FR animals had healthy, ID and FR feet.",yes
PMC11602436,Figure_1,oa_package/16/a7/PMC11602436.tar.gz,[],"FIGURE 1 (A) Optical microscopy. HE 20x. Thickening and stiffening of the capillary walls of the ball. (B)Silvermethanamine. Motheaten appearance as vacuoles or bubbles in the capillary wall. (C) Silvermethanamine. Spikes that prolong the glomerular basement membrane. (D)Immunohistochemistry. IgG4 positive, characteristic of primary membranous nephropathy.",yes
PMC10726427,Figure_2,oa_package/05/66/PMC10726427.tar.gz,"['The S163R L-ORD retina and normal ocular tissue from a 79-year old donor were subsequently stained with H E ( 2A and Supplemental S2).', '41 Side-by-side comparison of the RPE/choroid interface in aged albino or pigmented AAV-S163R injected mouse eyes and the 80-year old L-ORD donor retina shows the striking histologic resemblance to human ocular pathology in mice, with large eosinophilic basal deposits emanating from individual RPE cells, merging into a confluent layer ( 2 and Supplemental S3).', 'In advanced aged albino animals (15 months after injection), the prominent deposits consisting of the S163R mutant protein were associated with regions of significant retinal degeneration, similar to the human retina ( 2, B and C, and Supplemental s S4, S5, and S6).', ' 2Histologic comparison of the adeno-associated viral vector (AAV) S163R mouse model to human pathology.', '\nSupplemental S2Histologic examination of human retinal sections by light microscopy following hematoxylin and eosin staining.']","Figure2 Histologic comparison of the adeno-associated viral vector (AAV)S163R mouse model to human pathology. Hematoxylin and eosin (H&E)stained human paraffin retinal section from a previously reported 80-yearold S163R late-onset retinal degeneration (L-ORD) donor, showing the presence of a continuous layer of basal deposits, retinal pigmented epithelium (RPE) atrophy, and severe retinal degeneration. Representative H&E-stained mouse paraffin retinal section from a 17-monthold AAV-S163Rinjected albino mouse eye at 15 months following vector delivery, illustrating the remarkable resemblance to human disease, with similar eosinophilic subRPE deposits accumulating as a confluent layer and advanced retinal degeneration. Detection of the hemagglutinin (HA)tagged S163R mutant (green) in a 17-monthold AAV-S163Rinjected albino mouse eye. The S163R deposit was labeled by immunostaining with an anti-HA antibody followed by immunofluorescence microscopy. DAPI nuclear staining is shown in blue. Images were acquired by using a Zeiss Axiophot microscope. =4 mice ( ). Scale bars = 25 m ( ). D, deposit ( ); INL, inner nuclear layer; ONL, outer nuclear layer.",yes
PMC5963004,Figure_7,oa_package/00/18/PMC5963004.tar.gz,['Postinfection administration of MAb to PNAG reduces conjunctival PMN infiltration due to S.'],"Figure 7 Postinfection administration of MAb to PNAG reduces conjunctival PMN infiltration due to S. pneumoniae (D-39 and ATCC8) and S. aureus (LAC) after 48 hours of conjunctival infection in A/J mice. A total of 10 g of control IgG MAb or MAb to PNAG were injected into the conjunctiva 4, 24, and 32 hours postinfection in mice infected with S. pneumoniae D-39 or ATCC8 or S. aureus LAC. Control MAb-treated mice showed a large infiltrate of inflammatory cells and obvious edema in conjunctival areas, whereas mice treated with MAb to PNAG had only low levels of inflammatory cells and little edema. Arrows point to site of injection in the conjunctiva. Green bars: 10 m. Boxed areas, when shown, indicated magnified area.",yes
PMC4516482,Figure_2,oa_package/de/b6/PMC4516482.tar.gz,[],"Figure 2 p53 inhibition results in increased P2X R expression in primary cultured microglia. (A) Western blot analysis of P2X R protein (24 hrs after plating). (B) Real-time quantitative RT-PCR analysis of P2X R mRNA (6 hrs after plating). The p53 inhibitor pifithrin- (10 M) was pre-incubated with microglia for 30 min. before plating the cells on dishes. Data are mean S.E.M. of six to seven separate experiments (* < 0.05, ** < 0.01 compared with non-treated microglia, by one-way ). (C) Western blot analysis of P2X R protein in microglia treated with fibronectin and pifithrin- (24 hrs after plating). Pifithrin- (10 M) was applied to microglia immediately before plating the cells on dishes coated with fibronectin (FN; 10 g/ml).",yes
PMC8375002,Figure_7,oa_package/3a/c3/PMC8375002.tar.gz,"['27,28,30,61\nN-Bak in 661W cells was first examined by nested RT-PCR, which revealed the absence of the neuronal splice variant and the presence of the full-length variant in these cells (', 'The expression of full-length BAK protein was also confirmed by western blotting extracts from both wild type and Sg1 661W cells (', 'The siRNA significantly reduced the levels of BAK protein in both cell lines relative to cells treated with a scrambled siRNA (Figs.', '7C and 7D) but did not completely eliminate it.', '005, relative to Sg1 cells treated with scrambled siRNA) (', '.', '55,56 When neuroblasts switch to the splice variant is not known, but full-length BAK is expressed by glia in the CNS43,82,84 (note the presence of both the N-Bak and full-length transcripts in mouse brain in ']","Figure 7. Characterization of BAK expression and knock-down in WT and Sg1 661W cells. Nested PCR yields a 200 bp product for transcripts from the full length splice variant ( ), which is present in the liver, brain, and both wild-type (WT) and Sg1 661W cells. The brain sample also shows the neuronal ( ) splice variant containing a weak 20 bp exon. is not detected in 661W cells. Extraneous lanes were removed for presentation of this figure. Western blot showing full length BAK in spleen and both undifferentiated ( ) and differentiated ( ) 661W cells. Full length BAK is virtually undetectable in normal mouse retina. Western blot showing levels of BAK protein in differentiated WT and Sg1 661W cells treated with a scrambled siRNA or an siRNA directed against . Extraneous lanes were removed for presentation of this figure. siRNA results in depletion of BAK protein. Mean ( SD) of 4 independent western blots shown. (* 0.033). Graph showing the level of caspase 3 activation in cells expressing HDAC3-FLAG. All data were collected 24 hours after induction of differentiation. WT cells show a significant increase in activated caspase 3 staining after differentiation, which is not affected by scrambled siRNA. Nucleofection with siRNA; however, results in a significant reduction in cells with activated caspase 3. Sg1 cells also show a significant increase in activated caspase 3 staining after differentiation, but this increase is reduced relative to WT cells (no siRNA treatment, < 0.0001, not indicated on graph) consistent with the results in . Nucleofection of scrambled siRNA does not affect caspase 3 activation in 661W cells, but siRNA induces a further reduction in caspase 3 activation over Sg1 cells alone, and over WT cells nucleofected with the siRNA. Values shown are the mean (SEM) of three independent experiments. At least 120 cells were scored for each treatment group in each experiment. (* = 0.0052; ** 0.0005; *** < 0.0001).",yes
PMC4373156,Figure_1,oa_package/65/18/PMC4373156.tar.gz,"['The anteroposterior chest x-ray depicted a severely enlarged cardiac silhouette, ingurgitated hilar and interstitial pulmonary vasculature, and right pleural effusion ().', 'MorpariaKDickermanMHoehnKSFutility: unilateral decision making is not the default for pediatric intensivistsPediatr Crit Care Med201213e3111522760427.']",Figure 1. Chest X-ray at presentation. Antero-posterior chest-X ray incidence showed a normal cardiothoracic index with mild hilar ingurgitation and right pleural effusion.,yes
PMC10648501,Figure_4,oa_package/fe/ad/PMC10648501.tar.gz,"['As shown in A, strong A expression was found in the APP/PS1 retinas of both ages ((AII,IV,V)).', 'On the contrary, A staining was not observed in the retina of age-matched wt mice ((AI,III)).', 'Intracellular A deposits were detected in retinal ganglion cells (RGCs) but were also detectable in other neuronal classes in the inner nuclear layer (INL) ((AIV,V), arrow heads).', 'In the INL, we could observed A immunoreactivity in cell soma in this layer and a long projection to the ganglion cell layer (GCL) ((AIV,V), arrow heads).', 'As reported in A, immunofluorescent analysis of A staining revealed extensive A accumulation in the soma of RGCs ((AVI), arrow heads).', 'Moreover, co-immuno staining of A and lectin revealed retinal vascular A burden in the vessel wall and lumen area, representing A in blood mainly in the GCL but also in the outer nuclear layer (ONL) ((AVI,VII), arrows).', 'Quantitative analysis of A immunoreactivity confirmed significantly increased A deposits in 6- and 12-month-old APP/PS1mice compared with age-matched controls (B).', 'A deposits in retina of female APP/PS1 mice.']","Figure 4 A deposits in retina of female APP/PS1 mice. ( ) Representative photomicrographs of A deposition in retina of female 6- and 12-month-old APP/PS1 and age-matched wt mice stained with anti-A antibody and visualized with DAB labelling and hematoxylin counterstain ( ). In right panels, representative fluorescent images of retina stained for A (red), blood vessels (lectin, green), and nuclei (DAPI, blue) in female 6- and 12-month-old APP/PS1 and age-matched wt mice ( , ). Scale bar = 10 m. ( ) Quantitative analysis of A immunostaining showing significant increase in retinal A in female 6- and 12-month-old APP/PS1 and age-matched wt mice. Data expressed as media SEM. * < 0.05, *** < 0.001, n = six mice per group, using two-way ANOVA and Bonferronis multiple comparison post-test. wt: wild type, GCL: ganglion cell layer, IPL: inner plexiform layer, INL: inner nuclear layer, OPL: outer plexiform layer, ONL: outer nuclear layer.",yes
PMC5987922,Figure_2,oa_package/98/2f/PMC5987922.tar.gz,"[', 2017), we measured significant levels of IL-1 in serum of 4- to 7-wk-old MefvV726A/V726A mice, but not in heterozygous littermate control mice ( A).', 'Notably, deletion of GSDMD fully abolished in vivo IL-1 production in serum of MefvV726A/V726A mice aged 4 7 wk ( A), which contrasted markedly to the continued release of IL-1 in culture media of BMDMs that had been infected with C.', 'MefvV726A/V726A mice aged 10 wk and older continued to present with high IL-1 concentrations in their blood, whereas pyroptosis-deficient littermate mice had only background IL-1 levels ( A), identifying pyroptosis as the central mechanism of chronic IL-1 release in the circulation of MefvV726A/V726A mice.', 'Consistent with Pyrin inflammasome activation in vivo, we found that caspase-1 and GSDMD were constitutively activated in peritoneal macrophages and circulating monocytes of MefvV726A/V726A mice, but not in cells of MefvV726A/+ littermate mice (, B and C).', '.', 'Pyroptosis causes neutrophilia, runting, and wasting disease in MefvV726A/V726A miceTo further assess the role of GSDMD in IL-1 mediated autoinflammation, we examined signs of systemic neutrophilia, which is typically involved in the clinical manifestation of FMF (Ozen and Bilginer, 2014).']","Figure 2. Levels of IL-1 in serum samples taken from littermate mice of the indicated genotypes that were aged 47 wk and 1014 wk at the time of analysis. Data are presented as mean SEM with = 413 for each genotype. Data points are color coded according to the mouse genotypes shown in the corresponding figure legend. P < 0.05 is considered statistically significant. **, P < 0.01 and ****, P < 0.0001 using unpaired Students test and two-tailed p-values. ns, not significant. Blood monocytes and peritoneal macrophages from littermate mice of the indicated genotypes and age were immunoblotted for caspase-1, GSDMD, and -actin upon collection or after incubation in culture media for the indicated duration. Data are representative for three independent experiments. p in Western blots denotes protein molecular weight. Data are representative of three independent experiments with = 3 in each repeat (B and C).",yes
PMC9733815,Figure_2,oa_package/4a/4d/PMC9733815.tar.gz,"['Chest CT, sagittal viewThe yellow asterisk (*) indicates a large anterior mediastinal mass.']","Figure 2 Chest CT, sagittal view The yellow asterisk (*) indicates a large anterior mediastinal mass. The green arrow indicates a mass effect on adjacent cardiopulmonary structures.",yes
PMC11605016,Figure_3,oa_package/00/86/PMC11605016.tar.gz,"['T2 Axial MRI sequence of the ankle joint, showcasing a complete medial sleeve rupture with visible fluid between the sleeve and medial malleolus.']","Figure 3 T2 Axial MRI sequence of the ankle joint, showcasing a complete medial sleeve rupture with visible fluid between the sleeve and medial malleolus.",yes
PMC3389846,Figure_10,oa_package/62/55/PMC3389846.tar.gz,[],Figure 10. The scheme of pulmonary nodules scoring [ ].,yes
PMC11605744,Figure_1,oa_package/c1/20/PMC11605744.tar.gz,"['The tumour cells are large polygonal with round-to-oval nuclei, vesicular nuclear chromatin, mild nuclear pleomorphism and prominent nucleoli with moderate-to-abundant amounts of fine granular cytoplasm ((a) and (b)).', '.', '1177_2050313X241302255-fig1"" position=""float""/>.']","Figure1. A panel of microphotographs of malignant granular cell tumour: (a) Solid sheets of tumour cells, polygonal with abundant granular cytoplasm (H&E, 100); (b) Tumour cells showed mild-to-moderate nuclear pleomorphism, vesicular nuclei and prominent nucleoli (H&E, 400); (c) Spindle cell differentiation, short fascicles and moderate nuclear pleomorphism (H&E, 200); (d) Large areas of necrosis (H&E, 40).",yes
PMC4811449,Figure_2,oa_package/74/d6/PMC4811449.tar.gz,"['Nuclear SIRT1 (A) and SIRT7 (B) protein immunoexpression in endometrial carcinomas, with marked nucleolar staining in SIRT7 (Bar = 50 m)SIRT1 and SIRT7 protein immunoexpression in endometrial carcinomas and non-neoplastic endometria (Bar = 100 m)SIRT7 IHC expression was localized to the nucleus and presented either as homogeneous or nucleolar as shown in B.']","Figure 2 Nuclear SIRT1 (A) and SIRT7 (B) protein immunoexpression in endometrial carcinomas, with marked nucleolar staining in SIRT7 (Bar = 50 m)",yes
PMC4601438,Figure_2,oa_package/bc/86/PMC4601438.tar.gz,[],Figure 2 Diffuse type,yes
PMC3887218,Figure_3,oa_package/f1/7c/PMC3887218.tar.gz,"['A shows confocal microscopy images of K18*-488 (green) and FM 4-64 (red).', 'B is a comparison of uptake at 37 C (', 'In C, we show that incubating 100 m 1% K18*-488 for 24 h at 37 C does not cause any fluorescence lifetime drop.', '7, which represents the physiological environment inside endo- and lysosomes, we observed the formation of aggregates within 24 h and a corresponding decrease in fluorescence lifetime from 3824 22 ps to 3268 31 ps for K18*-488 and from 3765 53 ps to 3409 189 ps for hTau40*-488 (D), despite the absence of heparin.']","FIGURE 3. , SH-SY5Y cells were incubated with 1 10% K18*-488 ( ) for 24 h, and the lipid marker FM 4-64 ( ) was added during the last 15 min of incubation. The cells were imaged by confocal microscopy. Colocalization between FM 4-64 and K18*-488 is displayed in = 5 m. , SH-SY5Y cells were incubated with 1 10% K18*-488 ( ) either at 37 C ( ) or 4 C ( ). After 1 h, both dishes were trypsin-washed and imaged by confocal microscopy. The images display more internalized K18*-488 when the cells are incubated at 37 C rather than at 4 C. = 5 m. , K18*-488 was incubated for 24 h at 100 in cell culture medium and compared with K18*-488 incubated at 1 in cell culture medium. The fluorescence lifetimes measured were 3765 42 ps and 3664 25 ps, respectively. represent S.D. , K18*-488 was incubated at 1 (10% labeled) in cell culture medium at pH 4.7. hTau40* was incubated at 10 (1% labeled) in BES buffer at pH 4.7. The fluorescence lifetimes of soluble (3824 22 ps) and aggregated K18*-488 (3268 31 ps) and of soluble (3765 53 ps) and aggregated hTau40*-488 (3409 189 ps) indicate that low pH is sufficient to induce aggregation of both K18* and hTau40* in the absence of heparin. represent S.D. Unpaired Student's test statistical analysis was performed. ****, < 0.0001. , Alexa Fluor 488 was dissolved in cell culture medium to 100 n (pH 4.7 or 7.4), and the mean fluorescence lifetime was measured to be 3889 ps 28 ps and 3844 14 ps, respectively. represent S.D.",yes
PMC7977697,Figure_21,oa_package/de/90/PMC7977697.tar.gz,[],"Figure 10b: Probable usual interstitial pneumonia pattern. Axial inspiratory CT images demonstrate peripheral and basilar-predominant reticular abnormality, with mild peripheral traction bronchiectasis or bronchiolectasis, but without associated honeycombing, making this a probable UIP pattern based on Fleischner and American Thoracic Society guidelines. Coronal inspiratory CT scan confirms the lower lungpredominant distribution of disease in the craniocaudal plane.",yes
PMC11301062,Figure_2,oa_package/0b/dc/PMC11301062.tar.gz,"['2Histological analyses of liver, adipose and bone tissuesIn mice on a HFD, an increased average diameter of adipocyte cells, disorganized subcutaneous and visceral adipose tissues, larger quantities of lipid droplet accumulation in liver tissues, and elevated quantity of fat vacuoles in bone marrow were observed ().', 'Histological analyses of subcutaneous and visceral tissues, bone, and liver tissues.', ')']","Fig. 2 Histological analyses of subcutaneous and visceral tissues, bone, and liver tissues. (AD) Hematoxylin and eosin staining showed larger fat cell size in subcutaneous adipose tissues of mice fed a HFD. (EH) Larger fat cell size in visceral adipose tissues of mice fed a HFD. (I, J) More fat vacuoles in the bone marrow of mice fed on a HFD. (K, L) More lipid droplets accumulated in the liver tissues of HFD-fed mice with Oil Red O staining. CON, control; HFD, high-fat diet. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)",yes
PMC2270292,Figure_4,oa_package/56/95/PMC2270292.tar.gz,"['After 2 DSS, Winnie and Eeyore showed earlier faecal occult blood than did wild-type mice (after exclusion of mice with spontaneous rectal bleeding or prolapses), and higher histological colitis scores after 3 d (B), whereas by day 7 colitis scores in wild-type mice were closer to those of mutant mice.', 'Susceptibility of Mice with Muc2 Mutations to Dextran Sodium Sulphate Induced Colitis(A) Wild-type (WT) C57BL/6 and Winnie (Win) mice (n = 12) were given 0.', 'Mean haematocrits on day 7 were 41 for wild-type versus 28 for Winnie mice and 29 for heterozygotes (C).']","Figure 4 Susceptibility of Mice with Mutations to Dextran Sodium SulphateInduced Colitis (A) Wild-type (WT) C57BL/6 and ( ) mice ( = 12) were given 0.5% DSS in drinking water for 63 d. Body weight (mean standard deviation, ANOVA -value shown, Bonferroni's post-hoc test versus wild-type mice, *** < 0.001) and overall survival (Kaplan-Meier survival estimates and Mantel log-rank test [ -value]) are shown. (B) Wild-type C57BL/6 (WT), and ( ), and ( ) mice not manifesting rectal bleeding or prolapse ( = 6) were given 2% DSS in drinking water or water alone for 7 d. Histological colitis scores were determined on days 3 and 7 and faecal occult blood determined daily. Statistics: box plots show median, quartiles, and range; -values for Kruskal-Wallis nonparametric analysis are shown, Dunn's multiple comparison test versus WT, * < 0.05, ** < 0.01, *** < 0.001. (C) Wild-type C57BL/6, , and heterozygous ( /+) mice ( = 5) were given 3% DSS in drinking water or drinking water alone (CON) for 7 d, at which time body weight, colon length, and haematology were assessed. Statistics: box plots show median, quartiles, and range; -values for Kruskal-Wallis nonparametric analysis comparing DSS-treated WT, /+, and mice are shown, Dunn's multiple comparison test versus WT, * < 0.05, ** < 0.01; NS, not significant. Additional data from these experiments are shown in and .",yes
PMC5021458,Figure_1,oa_package/fb/cc/PMC5021458.tar.gz,"['MRI revealed TPS with loss of the posterior pituitary bright spot (s 1(a) and 1(b)).', '61MRI shows absence of posterior pituitary bright spot (a) and thickened pituitary stalk (b).']",Figure 1 MRI shows absence of posterior pituitary bright spot (a) and thickened pituitary stalk (b).,yes
PMC11599454,Figure_4,oa_package/19/00/PMC11599454.tar.gz,"['Immunofluorescence staining of the brain sections showed that the size of A plaques was significantly reduced in the CC-treated AD mice (', ' 4A), and further Western blot analysis showed that the protein level of A was also significantly decreased in brain tissues of CC-treated AD mice (', 'In addition, the A 1-42 and A 1-40 contents in the brain tissue were measured using ELISA and discovered that both A 1-42 and A 1-40 levels in the AD mice were reduced after CC treatment (', 'The protein levels of LC3-II were significantly increased in brain tissues of CC treatment AD mice (', 'Analysis of neuroinflammatory marker proteins also indicated that 12-month-old APP/PS1 mice contained higher levels of NOD-like receptor family pyrin domain-containing 3 (NLRP3) and the proinflammatory cytokine tumor necrosis factor-alpha (TNF- ) in the brain compared to the WT mice, while CC treatment obviously decreased the protein levels of NLRP3 and TNF- in the AD mice (', 'In contrast, the interleukin-10 (IL-10) antiinflammatory cytokine was increased after CC treatment (', "" 4CC treatment of 7-month-old APP/PS1 AD mice promotes clearance of A plaques and improves Alzheimer's disease symptoms."", 'In addition, to verify the effect of CC on the transcriptional activity of TFEB, qRT-PCR was performed using the brain samples, and demonstrated that the CC treatment upregulated the expression of TFEB downstream genes encoding LC3B, p62, LAMP2, CTSB, and CTSD, which are associated with autophagy and lysosomal function (']","Figure4 . , representative images of the hippocampus and cortex in mouse brain sections immunostained with an antibody against A and DAPI. The scale bar represents 50m. , mouse brains were extracted and used for Western blot analysis using antibodies against A, LC3 and NLRP3, -actin was used as a loading control. , the A and A contents in the mice brain tissues were detected using ELISA. The detected protein levels were quantified by densitometric analysis and are represented as mean band intensity normalized to -actin. The levels of the cytokines including TNF- ( ) and IL-10 ( ) in the brain tissues were detected using ELISA. , the mRNA levels of genes encoding LC3B, p62, LAMP2, CTSB, and CTSD in the brain tissue were detected by qRT-PCR analysis (n= 5). Data are presented as meanSD of three independent experiments. A, amyloid-; AD, Alzheimers disease; APP, A precursor protein; CC, clomiphene citrate; CTSB, cathepsin B; CTSD, cathepsin B; DAPI, 4,6-diamidino-2-phenylindole; LC3, microtubule-associated protein light chain-3; NLRP3, NOD-like receptor family pyrin domain-containing 3; qRT-PCR, quantitative reverse transcription PCR; TNF-, tumor necrosis factor-alpha.",yes
PMC6899892,Figure_6,oa_package/8f/e2/PMC6899892.tar.gz,"['Immunocytochemistry: 4 ,6 diamidino 2 phenylindole (DAPI) (blue), vimentin (red), smooth muscle actin ( SMA) (green).']","Figure 6 Immunocytochemistry: 4,6diamidino2phenylindole (DAPI) (blue), vimentin (red), smooth muscle actin (SMA) (green). Ligamentum flavum shows fibroblastlike morphology with vimentin stain (A). The transforming growth factor1 (TGF1)stimulated group shows myofibroblastlike morphology with SMA stain (B). The CCN5+TGF1 group shows similar cell morphology and stain characteristics to control rather than the TGF1 group. [Color figure can be viewed at wileyonlinelibrary.com]",yes
PMC8287342,Figure_1,oa_package/f5/6a/PMC8287342.tar.gz,"['Radiographs showed a calcaneal tuberosity osteolytic lesion with marginal sclerosis\nand no associated periosteal reaction ().', '.', '1177_2050313X211027094-fig1"" position=""float""/>.']",Figure 1. (a) Preoperative soft tissue mass on lateral region of right calcaneus. (b)Preoperative lateral radiograph showing a lytic lesion on calcanealtuberosity without periosteal reaction. No acute fracture was noted.,yes
PMC8206194,Figure_3,oa_package/a1/10/PMC8206194.tar.gz,[],"Fig. (3) Other potential targets for osteoporosis therapy have been identified and against some of them have been produced monoclonal antibodies or recombinant analogues. In addition to anti-sclerostin monoclonal antibody (Anti-sclerostin MoAb) and Anti-tumor necrosis factor- monoclonal antibody (Anti-TNF- MoAb), other relevant checkpoints of the Wingless/Integrated (Wnt) pathway in osteoblasts seem to be promising targets: Dickkopf-related protein (DKK) antagonists, Proteasome inhibitors, Tyrosynkinase inhibitors (TKIs), Recombinant Bone morphogenetic protein (rBMP), Tryptophan hydroxylase 1 (Tph1), Recombinant transforming growth factor- (rTGF-), Recombinant insulin-like growth factor-1 (rIGF-1), Secreted frizzled-related protein 1 (SFRP1) antagonists. Abbreviations: Tumor Necrosis Factor (TNF), Interleukin-1 (IL-1), Receptor Activator of Nuclear-factor k-B (RANK), Receptor Activator of Nuclear-factor k-B Ligand (RANKL), Osteoprotegerin (OPG), Tumor Necrosis Factor receptor-associated Factor 6 (TRAF6), Inhibitor of nuclear factor Kappa-B Kinases (IKKs), Nuclear factor-kB (NF-kB), Nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1). ( ).",yes
PMC4717431,Figure_1,oa_package/47/b3/PMC4717431.tar.gz,"['According to Laur n, nine (56%) RHOA mutant GCs had a diffuse, four (25%) an intestinal, two (13%) an unclassified and one (6%) a mixed phenotype (figure 1).']","Figure1 Histomorphology of gastric cancers with mutation. mutant gastric cancers were most commonly of diffuse type according to Laurn (A). Intestinal (B), unclassified (C) and mixed (D) phenotype were found in a minority of the cases. H&E; original magnification 200-fold.",yes
PMC8591691,Figure_5,oa_package/6b/65/PMC8591691.tar.gz,"['The histopathological examination of the surgical samples confirmed the neoplastic nature of the nodule, mainly organized in microfollicular structures, although an insular, solid and less differentiated pattern occurred in ~30% of thyroid carcinoma (A and B); partial invasion of fibrous capsule as well as of 4 invasive vessels neoplastic foci were recorded.', 'Histopathological analysis.']","Figure 5 Histopathological analysis. The neoplasm revealed a (A) greater extension of microfollicular structures, while (B) solid and less differentiated areas were elsewhere appreciable (hematoxylin and eosin staining; magnification, x240).",yes
PMC6948954,Figure_5,oa_package/e2/84/PMC6948954.tar.gz,[],"Figure 1figure supplement 4. Proximity of VEGF-C expression during murine kidney development. ( ) Wholemount X-gal staining of E15.5 embryonic kidneys from mice. Scant beta-galactosidase (-gal) was observed in control kidneys, whereas a vascular pattern of staining, originating from the renal hilum (white arrow) was observed in kidneys. Scale bar: 500 m ( ) DAB immunohistochemical staining for LYVE-1 of serially sectioned X-gal-stained E16.5 kidneys. Image contrast was adjusted in FIJI to best distinguish the 3,3-DAB (brown) and -gal (blue), by setting display values in all images to a min/max of 33/222. The left panel shows strong -gal activity in the hilum and around arterioles (black asterisk) of E16.5 kidneys. The middle inset shows a high-power image of the region delineated with a black box. Immunoreactivity for LYVE-1 is detectable in the membrane of a lumenized lymphatic vessel which is surrounded by -gal-expressing interstitial cells. This is comparable to the right panel, an unstained serial section of the same lymphatic vessel demonstrating no immunoreactivity. Scale bars: 20 m. A, arteriole; G, glomerulus; H, hilum; L; lymphatic vessel lumen, U, ureter.",yes
PMC4330237,Figure_3,oa_package/b9/9b/PMC4330237.tar.gz,"[' 3), which form from those hard tissues located on the opposite side of the IL between the X-ray source and the centre of rotation.', 'Main anatomical ghost shadows in a PTG: 1, contralateral angle and body of the mandible; 2, cervical spine; 3, contralateral hard palate.', 'Polypoid swelling or retention cyst in the alveolar recess of the right maxillary sinus\nDetailed knowledge of the anatomy is the key to recognising pathology.']","Fig. 3 Main anatomical ghost shadows in a PTG: 1, contralateral angle and body of the mandible; 2, cervical spine; 3, contralateral hard palate. Note missing or extracted dd. 15, 38, 48; persistent d. 65 and peg-shaped d. 22. Polypoid swelling or retention cyst in the alveolar recess of the right maxillary sinus",yes
PMC6555576,Figure_2,oa_package/69/2f/PMC6555576.tar.gz,"['It starts from the fetal\nabdomen, from the infradiaphragmatic region to the upper mediastinum, obtaining\n6 planes, as shown in .', '\n.', 'Both arches should present flow in the same direction, always\ndirected from the heart toward the descending thoracic aorta (.', '\n.', '4), bicaval view\n(.', '\n.', '\n\n.', '\n\n.', '\n\n.', '\n\n.']","Figure 2.2 Aorta and pulmonary artery appear elongated, going toward thedescending aorta. Both converge to the aorta forming an imagesimilar to a V letter. The trachea is to the right of the aorticarch, demonstrating that the latter descends to the left. Duringcolor flow mapping, both arches are observed to have flow in thesame direction, i.e., from the heart toward the descendingthoracic aorta. AoA: aortic arch; DA: ductal arch; SVC: superior vena cava; T:trachea.",yes
PMC7364792,Figure_2,oa_package/a1/aa/PMC7364792.tar.gz,"['However, peripheral sheet-like detachment and desquamation involving 30% of her body surface area were observed, with positive Nikolsky sign ().', '.', '1177_2050313X20940420-fig2""/>The differential diagnosis at this time included classic TEN, TEN-like LE, TEN-like presentation of linear IgA bullous dermatosis (LABD), pseudoporphyria, generalized fixed drug eruption (GFDE) and drug reaction with eosinophilia and systemic symptoms (DRESS).']",Figure 2. Peripheral sheet-like detachment and desquamation on follow-up the next day.,yes
PMC11216641,Figure_4,oa_package/d5/2d/PMC11216641.tar.gz,"['jcvjs_167_23-f004"">T2 axial (a) and sagittal (b) showing calcified sequestrated disc (arrow)The overall mean size of CTDs was 806.']",Figure 4 T2 axial (a) and sagittal (b) showing calcified sequestrated disc (arrow),yes
PMC9741155,Figure_4,oa_package/b5/a7/PMC9741155.tar.gz,"['Based on a radiographic examination, a mild but decreased mineralization of the skeleton and a large, excessively mineralized egg were seen in the left caudal coelomic cavity, while in the right caudal coelomic cavity, the remnants of an eggshell could be noticed (A,B).', 'Ventrodorsal (A) and laterolateral (B) radiograph of a black marsh turtle (Siebenrockiella crassicollis) showing mild generalized decreased mineralization of the skeleton and a large, excessively calcified egg in the left caudal coelomic cavity as well as the remnants of an eggshell (arrow) in the right coelomic cavity.']","Figure 4 Ventrodorsal ( ) and laterolateral ( ) radiograph of a black marsh turtle ( ) showing mild generalized decreased mineralization of the skeleton and a large, excessively calcified egg in the left caudal coelomic cavity as well as the remnants of an eggshell (arrow) in the right coelomic cavity.",yes
PMC11445596,Figure_2,oa_package/5a/67/PMC11445596.tar.gz,"[':Time-of-flight magnetic resonance venography oblique axial view in a 27-year-old male showing persistent occipital sinus (orange arrow), draining into the jugular veins (green arrows) via marginal sinus (yellow arrow).']","Fig. 2 Time-of-light magnetic resonance venography oblique coronal view in a 27-year-old male showing persistent bilateral occipital sinus (orange arrows), draining into the bilateral jugular veins (green arrows) via bilateral marginal sinus (yellow arrows). There is an absence of a bilateral transverse sinus. Bilateral sigmoid sinus (blue arrows), superior sagittal sinus (gray arrow), straight sinus (red arrow), and condylar vein are visualized (purple arrow).",yes
PMC9561687,Figure_4,oa_package/b5/e8/PMC9561687.tar.gz,"[' 4A, Supplementary Data S3).', ' 4B).', ' 4B).', ' 4B).', ' 4B).', ' 4B).', ' 4C,D).', 'Multiple types of programmed necrosis are active simultaneously in the IR retina.', 'Mlkl- and gasdermin- dependent types of programmed necrosis contribute significantly to IR-induced retinal injuryNecroptosis, or Mlkl-dependent programmed necrosis, occurs after an inflammatory cytokine Tumor Necrosis Factor alpha (TNF/Tnf) or DAMPs, bind to the receptors Tnfr1/Tnfrsf1a, Tlr4, and Tlr3 (the latter two are linked to the TRIF/Ticam1 signaling cascade), causing the necrosome (primarily composed of Ripk1, Ripk3, and Mlkl proteins) to form.', ' 4).']","Figure 4 Multiple types of programmed necrosis are active simultaneously in the IR retina. ( ) The results of the pathway analysis revealed that many genes with increased expression in the ischemic retina are involved in programmed (regulated) necrosis (red arrows indicate pro-inflammatory signaling cascades, dark red arrows indicate signaling cascades involved in programmed necrosis). ( ) The expression of many genes regulating necroptosis, pyroptosis, oxytosis/ferroptosis, and parthanatos is significantly increased in the ischemic retina 6 and 24h after reperfusion. We highlighted in dark red those genes whose expression was significantly increased (two-fold or more) in IR retinas. ( , ) The representative KEGG pathway maps show increased expression of necroptosis, oxytosis/ferroptosis, and parthanatos genes in the IR retina. The red box corresponds to increased gene expression in the ischemic retina, the green box corresponds to reduced gene expression in the ischemic retina.",yes
PMC3150139,Figure_3,oa_package/db/9b/PMC3150139.tar.gz,"['01) respectively versus their T/SS lymph infused counterparts (A).', 'In contrast, there was no significant increase in the percentage of EBD in the BALF of TRIFmut mice infused with T/HS lymph relative to their T/SS lymph infused group (B).', 'g003TRIF and Myd88 deficiency confer full and partial protection against T/HS lymph induced microvascular permeability.']",10.1371/journal.pone.0014829.g003,yes
PMC3088377,Figure_11,oa_package/2a/33/PMC3088377.tar.gz,[],"Figure 11. Hemorrhagic brainstem infarct. (A) Gradient echo axial magnetic resonance image depicts a focus of hypointensity due to paramagnetic effect of the hemosiderin, otherwise known as blooming. (B) Axial computed tomography through the brainstem demonstrates a corresponding hyperattenuating focus of hemorrhage (arrow) in the brainstem.",yes
PMC10537798,Figure_1,oa_package/51/62/PMC10537798.tar.gz,"['Skin and MuscleAnalysis of pathoanatomic changes in the skin, subcutaneous tissue, and musculature of cattle infected with various isolates (Dagestan/2015, Udmurtiya/2019, and Saratov/2017) of LSDV shows that, in all cases, there are pronounced focal changes in the skin and subcutaneous tissue (, and ).', 'Lymph NodesThe isolates of Dagestan/2015 and Udmurtiya/2019 caused damage to the lymph nodes, and, in the case of the addition of purulent microflora, purulent exudate is formed (1, 2 and 3).', '\nLumpy skin disease-a manual for veterinariansFAO Animal Production and Health ManualFAORome, Italy2017Volume 20Pathological changes in skin, subcutaneous, and muscle tissues in cattle infected with LSDV Dagestan/2015.', '1Serous lymphadenitis of the pharyngeal lymph nodes with spot hemorrhages.', '2Serous lymphadenitis.', '3Focal purulent lymphadenitis with Udmurtiya/2019.']","Figure 1 Pathological changes in skin, subcutaneous, and muscle tissues in cattle infected with LSDV Dagestan/2015. ( ) Multiple focal necrotic dermatomyositis. ( ) Focal and fine-spotted hemorrhages in subcutaneous tissue. Subcutaneous tissue is moist and shiny, with multiple jagged-edged red foci of various shapes and sizes on the periphery; local, small hemorrhages. Areas with affected outer layers of muscle were observed as red-colored muscle lesions of irregular sizes and shapes.",yes
PMC6769081,Figure_7,oa_package/b7/e7/PMC6769081.tar.gz,"['Interestingly, VCAM1 levels that are enhanced in 5XFAD-WT mice were diminished in 5XFAD-MPO KO mice (E).', 'FIGURE S2Full gel image of APOE western blot analysis shown in A.']","FIGURE 7 Low levels of inflammatory markers in 5XFAD MPO KO mice. mRNA expression analysis of IL1 , CXCL10 , CCL2 , TNF , and VCAM1 in hippocampal samples. Data are mean SEM. < 0.05 and < 0.01. Two-tailed Mann-Whitney test.",yes
PMC7526559,Figure_2,oa_package/76/9e/PMC7526559.tar.gz,"['05, while none of these changes were observed in HF alone (Supplemental and Supplemental Table 9).', 'Similarly, 19 transcripts were dysregulated (6 up and 13 down) in the LA CMN of AF+HF versus NF samples, and the majority of these changes (63%) also were not seen in HF alone (Supplemental and Supplemental Table 9).', 'At baseline, there were no differences in ploidy between the LA and RA in NF hearts, with a distribution of approximately 47% 2n cardiomyocytes, 45% 4n, and 7% 4n at baseline in both atrial chambers (A).', 'Similarly, in HF alone, the ploidy distribution was similar between the RA and LA (B).', 'In contrast, in the setting of AF+HF, there was a relative reduction of 2n nuclei in the LA, with concomitant increases in both the 4n and 4n LA CMN populations (C).', 'Here, we provide a resource detailing AF-associated GWAS loci and TF expression changes specifically within human right versus left atrial CMN, which may be used in guiding future cell type specific experimentation related to AF mechanisms (Supplemental , Supplemental Tables 9 and 10).', 'We provide the first evidence to our knowledge that ploidy is increased in human atrial CMN in the setting of AF+HF, specifically in the LA ().', 'CM ploidy is increased in LA CMs of humans with AF.']","Figure 2 CM ploidy is increased in LA CMs of humans with AF. CMN were isolated from the RA and LA of NF, HF, and AF+HF. Nuclei were sorted based on DAPI and PCM1 staining, and distinct ploidy populations (2n, 4n, >4n) were identified based on differences in DAPI intensity ( ). ( ) CMN ploidy in NF RA versus NF LA is similar. ( ) CMN ploidy in HF RA versus HF LA is also similar. ( ) Comparison of ploidy in AF+HF RA versus AF+HF LA shows loss of 2n nuclei, with concomitant increase in 4n and >4n or higher ploidy nuclei in the LA in the setting of AF. Ploidy experiments were performed on 2 sample batches: batch 1: ( and ) = 9 NF RA, = 9 NF LA, = 4 AF+HF RA, = 6 AF+HF LA; batch 2: ( ) = 3 HF RA, = 3 HF LA. Data are presented as the mean from each group SEM. < 0.05 was considered statistically significant. * < 0.05; NS, not significant. Unpaired Students 2-tailed test with a Welchs correction was performed for all comparisons.",yes
PMC11602985,Figure_1,oa_package/2c/dd/PMC11602985.tar.gz,"['02) (', '', 'Using cross-sectional analyses of APOB mRNA and APOB protein levels in the frontal cortex in asymptomatic, MCI and AD patients from the ROSMAP cohort, we found an inverse relationship between mRNA and protein concentrations as a function of cognitive status (MMSE or Diagnosis, ', 'The increase cortical levels of APOB protein in MCI and AD cases (', 'Therefore, APOB progressively accumulates in cortical structures in late-stage AD (Braak IV-VI) causing a concomitant compensatory reduction in APOB mRNA prevalence (']",Fig.1 Brain APOB protein and mRNA levels exhibit opposite associations with clinical and pathophysiological makers of AD. Bar graph depicting brain APOB protein and mRNA levels taken from ROSMAP cohort. A: Protein levels were normalized by a log2 transformation. B: Messenger RNA levels were measured by fragments per kilobase of transcript per million mapped reads (FPKM). Quantitative measures of AD pathology (Braak and CERAD) were based on pathologic criteria for AD. Error bars represent one standard error.,yes
PMC2193459,Figure_6,oa_package/81/e8/PMC2193459.tar.gz,"['Primary cultures of synovial fibroblasts, derived from clinically unaffected knees of gp130 STAT/ STAT mice, showed an enhanced rate of [3H]thymidine incorporation in response to IL-6 or LIF when compared with cells obtained from gp130wt/wt mice ( A).', 'Furthermore, mutant cells responded to 100- to 1,000-fold lower concentrations of cytokines than wt cells and showed sustained IL-6 dependent activation of gp130-mediated signaling, as demonstrated by autophosphorylation of Jak-2, tyrosine phosphorylation of SHP-2, and phosphorylation of MAPK isoforms Erk1/2 ( B).', 'f5""/>Hyperresponsiveness of synovial cells correlates with sustained Erk MAPK activation.']","Figure 6 Hyperresponsiveness of synovial cells correlates with sustained Erk MAPK activation. (A) [ H]thymidine incorporation by synovial cells prepared from wt and gp130 mice and stimulated with LIF or IL-6. Cells derived from clinically unaffected joints were stimulated for 24 h with the indicated concentration of LIF or IL-6 in the presence of sIL-6R (500 ng/ml). Each point represents the mean SD. * < 0.05 compared with unstimulated cultures of the same genotype. (B) Activation of intermediate signaling molecules in synovial cells stimulated with IL-6 and sIL-6R (both at 500 ng/ml) for the indicated period of time. Cell lysates were immunoprecipitated with either a Jak-2 antiserum and incubated with -[ P]ATP to assess Jak-2 autophosphorylation, or with a SHP-2 antiserum and blotted with antiphosphotyrosine antibodies. Erk MAPK activation was analyzed by directly probing lysates with an antibody specific for phospho-Erk1/2 (Erk-P). The total amount of SHP-2 and Erk proteins was assessed by reprobing the membranes for SHP-2 and Erk1/2, respectively.",yes
PMC4189057,Figure_2,oa_package/02/69/PMC4189057.tar.gz,"['MRI showed a T2 bright left groin mass with edema along the fascial planes, and lower extremity angiogram demonstrated occlusion of the left common femoral artery with distal profunda femoris and mid-superficial femoral artery reconstitution ().', '(A) Left common femoral artery occlusion with distal profunda femoris reconstitution with (B) small and underperfused superficial femoral artery with mid-vessel reconstitution.']",Fig. 2 (A) Left common femoral artery occlusion with distal profunda femoris reconstitution with (B) small and underperfused superficial femoral artery with mid-vessel reconstitution.,yes
PMC5657061,Figure_4,oa_package/30/de/PMC5657061.tar.gz,"[' 4, 5).', '', ' 4A cross-section of the denervated renal artery showing increased inflammation around the intima and destroyed nerves\n']",Fig.4 A cross-section of the denervated renal artery showing increased inflammation around the intima and destroyed nerves,yes
PMC10015402,Figure_17,oa_package/3b/ce/PMC10015402.tar.gz,[],"Figure 17 Upper abdominal and thoracic right para-sagittal bicaval view shows the interrupted inferior vena cava with azygos continuation (normal position of IVC is marked by asterisks). The anterior hook-like structure is the morphological LAAp in left atrial isomerism. (Since the dilated azygos vein draining into the superior vena cava is more medially located, it is not visible in this plane.) The HV is directly connected to the RA. AzV: Azygos vein, DV: Ductus venosus, IAS: Interatrial septum, LA: Left atrium, PV: Pulmonary vein, RPA: Right pulmonary artery, Sp: Spine, UV: Umbilical vein, LAAp: Left atrial appendage, HV: Hepatic veins, RA: Right atrium",yes
PMC7571513,Figure_32,oa_package/a1/72/PMC7571513.tar.gz,[],Figure 32 Trochanteric bursitis. Coronal short-tau inversion recovery magnetic resonance image demonstrating distension of the greater trochanteric (short arrows) and subgluteus medius (long arrow) bursae consistent with bursitis,yes
PMC6066957,Figure_2,oa_package/37/f9/PMC6066957.tar.gz,"['2"">APP Copy Number Has a Significant Impact on the Transcriptome of DS Cortical Neuronal CulturesHierarchical clustering of microarray transcriptome data from DS APP+/+/+ and isogenic euploid day 65 samples shows clustering is dictated by the presence of Hsa21 ( 2A).', 'As expected, Hsa21 genes are highly overrepresented among significantly overexpressed transcripts (green dots in volcano plot in 2D), and day 45 gene expression data show similar trends (s S2D and S2E).', '0002), particularly those in the distal part of the long arm ( 2C).', 'Hierarchical clustering analyses revealed that the impact of the supernumerary APP copy number on the trisomy 21 transcriptome increases significantly from day 45 ( S2D) to day 65 ( 2A).', 'By day 65 about half of all genes downregulated upon inactivation of the supernumerary APP gene in DS neurons were also abnormally upregulated in DS neurons ( 2B).', 'This substantial impact of the APP gene on DS (Hsa21-trisomy)-associated gene expression changes in neurons particularly affects the expression of other Hsa21 genes (s 2C and 2D).', ' 2Transcriptome Profiling of Isogenic iPSC-Derived Neuronal Cultures at Day 65 of Differentiation(A) Hierarchical probe clustering-based heatmaps (with limits set at 1.']","Figure1 Manipulating Levels in Human Pluripotent Stem Cells Using CRISPR/Cas9-Aided Approaches (A) Vector design for targeting exon 3 of the gene. Green is PCR product, yellow rectangle is location of the Southern probe. gRNA, guide RNA; KO, knockout. (B) Targeted allele-specific PCR. (C) Southern blot showing targeted allele in DS18 iPSC. KI, knockin; WT, wild-type. (D and E) (D) Inactivationof one of the three alleles in day 45 DS iPSC-derived neurons reduces APP protein expression to isogenic euploid control levels (quantified in E, N= 3). (F) Doxycycline induced upregulation of HA-tagged dCas9-VP64 in Gen22::TRE-dCas9-VP64 with HA antibody. (G) Doxycycline induced APP protein expression in Gen22::TRE-dCas9-VP64 hESC (APP gRNA#1 shown). p< 0.01, p< 0.001, #non-significant; n= 3, n= 3 for qPCR and western blots. Means SEM values shown.",yes
PMC4230233,Figure_1,oa_package/f0/92/PMC4230233.tar.gz,['Mesenchymal stem cells (MSCs) ameliorate rhabdomyolysis (RM)-induced acute kidney injury (AKI).'],"Figure 1 Compared with animals treated with saline, infused MSCs significantly reduced serum creatinine (SCr ), blood urea nitrogen (BUN ) and serum phosphocreatine kinase (CK ) levels 24, 48 and 72hours after RM, and there were no significant changes following treatment of sham mice. * <0.05 versus sham, <0.05 versus rhabdomyolysis treated with saline at the corresponding times (n=10). MSC therapy markedly reduced acute tubular necrosis (ATN) scores 72hours after rhabdomyolysis. Treatment with the sham-operated control (Sham+NS and Sham+MSCs) had no effect on renal histopathological parameters , while MSC therapy markedly improved tubular injury , and mice treated with saline showed severe tubular injury (HPFs, 400, n=10). HPFs, high-power fields; NS, normal saline.",yes
PMC11157984,Figure_2,oa_package/be/0f/PMC11157984.tar.gz,"['This construct was expressed in HEK293T cells and compared with a construct encoding mCEL, the normal mouse CEL protein (Supplementary A).', 'The mCEL-HYB1 protein was less secreted into the medium than mCEL (Supplementary B).', 'Moreover, the abundance of mCEL-HYB1 was lower in the soluble cell lysate and higher in the insoluble pellet compared with mCEL (Supplementary B).', 'We also measured the enzyme activity in the conditioned media, finding that mCEL-HYB1 exhibited around 50% reduced lipase activity compared to the normal mouse enzyme (Supplementary C).', 'For homozygous Cel-HYB1 male mice, we observed a significant increase in body weight compared to controls at age 6 months but not 12 months (A).', 'Relative pancreatic mass was similar between the genotypes (B).', 'At 6 months of age, a significant increase in the relative mass of both white (epididymal and subcutaneous) and brown adipose tissue was noted in mutant male mice compared to controls (C).', 'Compared to controls, our Cel-HYB1 mice showed differences in the mass of fat depots also outside the pancreas (C, Supplementary ', 'pdf"" id=""d67e1508"" position=""anchor""/>Supp .']",Figure 2. SVC angioplasty and stenting.,yes
PMC9013596,Figure_8,oa_package/59/38/PMC9013596.tar.gz,[],FIGURE 8 (A) Welldifferentiated squamous cell carcinoma (H&E 100X) formed of multiple malignant squamous cell nests with central keratin pearls in more than 75% of tumour. (B) Squamous cell carcinoma of moderate differentiation showing cell nests with keratin pearls in 50% of tumour (H&E 100X). (C) Microscopic pathologic picture of poorly differentiated squamous cell carcinoma showing less than 25% cell nests with keratin pearls (H&E 100X),yes
PMC11384032,Figure_2,oa_package/76/22/PMC11384032.tar.gz,"['Arrow: tumoral necrosis The arrow points tumor proliferation with solid architecture (HE staining, 40) Tumor proliferation with solid architecture, papillary areas of necrosis (HE staining, 100).']","Figure 2 On the left side, inferior, tumoral proliferation with solid architecture with marked cellular pleomorphism (HE staining, 200). Arrow: tumoral necrosis",yes
PMC7535867,Figure_1,oa_package/36/e7/PMC7535867.tar.gz,['Gadolinium-enhanced T1-weighted coronal MRI: subtle right pituitary hypo-enhancement (white arrow) with elevation of diaphragmatic sella (red arrow)MRI: Magnetic Resonance ImagingA sagittal T1 weighted MRI view revealed the usual posterior pituitary high intensity signal ().'],Figure 1 Gadolinium-enhanced T1-weighted coronal MRI: subtle right pituitary hypo-enhancement (white arrow) with elevation of diaphragmatic sella (red arrow) MRI: Magnetic Resonance Imaging,yes
PMC6296180,Figure_3,oa_package/f1/5e/PMC6296180.tar.gz,"['(A) A segment of glomerulus showing endocapillary hypercellularity, capillary wall double contours, and wire loop lesions (H E stained, 200 ).']","Figure 3 ( Lupus nephritis class III. Light micrograph showing a glomerulus with segmental endocapillary hypercellularity, mesangial hypercellularity, and capillary wall thickening (H&E stained, 200). P53 nuclear immunostaining of apoptotic cells in class II of cLN, score I/II (200). Ki-67 nuclear immunostaining of mesangial proliferative glomeruli in class II of cLN, score I/II (200). Mild to moderate mononuclear tubulointerstitial nephritis (H&E stained, 200). cLN, childhood lupus nephritis.",yes
PMC9356240,Figure_6,oa_package/5c/6b/PMC9356240.tar.gz,"['5% in untreated Dup2 (s 6A and 6B; Table S8).', '2% at 6 months in the treated Dup2 (s 6A and 6B; Table S8).', 'Functional correction appeared more complete at the 6-month time point, where mean force loss at the final ECC in ACCA-treated Dup2 mice was not statistically different from that in Bl6 mice, further providing evidence of the longevity of the treatment (s 6C and 6D; Table S8).', ' 6Early systemic delivery of scAAV9.', 'Muscle sections from ACCA-treated animals appeared histologically normal with no significant signs of necrosis or immune cell infiltration, and treated muscle revealed almost no central nucleation, indicating that the early treatment prevented muscle degeneration for at least 6 months (s 6E and 6F).']","Figure5 Early systemic delivery of scAAV9.U7.ACCA in neonatal Dup2 mice drives long-lasting exon 2 skipping and dystrophin expression (A) RT-PCR quantification of tibialis anterior (TA), gastrocnemius (Gas), triceps (Tri), heart, and diaphragm (Dia) RNA from young Dup2 mice treated with 3.210 vg/kg scAAV9.U7.ACCA (ACCA) 1, 3, and 6months post injection. Data are presented as mean SD (n= 49 per group). (B) Western blots of triceps dystrophin in young ACCA-treated Dup2 mice. The standard curve on the right shows incremental dilutions of pooled Bl6 samples in dystrophin-null muscle lysate. (C) Quantification of western blots shown in (B), presented as mean SD with individual points. The dashed line indicates 100% based on the standard curve. Dup2 Tri control group is reproduced from B. (D) Representative images of dystrophin immunofluorescence (IF) in heart and triceps sections, and quantification of fiber dystrophin positivity in triceps. Images were processed identically with shading correction, rolling-ball background subtraction, and denoising (see for unprocessed images). Color-coded heatmaps reflect the percent dystrophin-positive perimeter for each muscle fiber, as indicated by the color scale. Fibers that have dystrophin around 30% of the perimeter are considered dystrophin positive. Scale bars, 200m. (E and F) Quantification of dystrophin IF intensity and percent dystrophin-positive fibers (PDPF) in muscle and heart sections, presented as mean SD with individual points. Untreated Dup2 and Bl6 groups show all tissues together. No statistically significant differences were identified between 3- and 6-month expression by unpaired t test (western blot) and two-way ANOVA (IF).",yes
PMC6209799,Figure_3,oa_package/26/1c/PMC6209799.tar.gz,"['Axial computed tomography scans show (a) the newly diagnosed pulmonic primary lesion, and after (b) one week and (c) two months of crizotinib therapy.']","Figure 3 Axial computed tomography scans show ( ) the newly diagnosed pulmonic primary lesion, and after ( ) one week and ( ) two months of crizotinib therapy.",yes
PMC10390820,Figure_4,oa_package/7e/5f/PMC10390820.tar.gz,"['However, if the biceps pulley tissue is intact (Figs 4 and 5) and the long head is stable, then isolated subscapularis repair is performed.', 'Right shoulder view from posterior portal, with patient in beach-chair position, with loop grasper through anterior working portal, showing full-thickness tear of upper border of subscapularis with retained intact biceps pulley tissue.']","Fig 4 Right shoulder view from posterior portal, with patient in beach-chair position, with loop grasper through anterior working portal, showing full-thickness tear of upper border of subscapularis with retained intact biceps pulley tissue.",yes
PMC4989573,Figure_5,oa_package/83/27/PMC4989573.tar.gz,[],Figure 5 Closer view of left mandibular molar region on sagittal cone-beam computed tomography image revealed radiopaque lesion covered with radiolucent band,yes
PMC10403632,Figure_2,oa_package/a2/ab/PMC10403632.tar.gz,"['M-mode TTE demonstrated the mass obstructing the MV orifice during diastole ( 2A).', 'Similarly, mitral regurgitation (MR) was considered mild to moderate ( 2B).', ' 2(A) Two-dimensional TTE (top) and M-mode imaging line through the MV (bottom) showing echogenic signal posterior to the anterior MV leaflet during diastole consistent with obstruction and protrusion of myxoma into the left ventricular chamber visually (white arrow).']","Figure1 Two-dimensional TTE, apical four-chamber view, visually demonstrating biatrial masses ( , right mass attached to the interatrial septum; , left-sided mass attached to the interatrial septum) with protrusion through the atrioventricular valves during diastole and near obliteration of the atrial cavity during systole . , Left atrium; , left ventricle; , right atrium; , right ventricle.",yes
PMC6024485,Figure_1,oa_package/bc/d8/PMC6024485.tar.gz,['Digital radiography showed L4 vertebral spondylolisthesis and right hip prosthesis.'],Figure 1 Digital radiography showed L4 vertebral spondylolisthesis and right hip prosthesis.,yes
PMC3955308,Figure_2,oa_package/18/59/PMC3955308.tar.gz,"['The teeth were obturated with Gutta-percha after 1 year [].', 'Post-operative periapical radiograph of 11, 12 and 22Panoramic radiograph taken 5 years after the initial traumaPeriapical radiograph of 11, 12 and 22 taken 5 years after the initial traumaPeriapical radiograph of the same teeth taken 7 years after the initial traumaDISCUSSIONThe management of traumatically intruded incisors is challenging.']","Figure 2 Post-operative periapical radiograph of 11, 12 and 22",yes
PMC4928349,Figure_3,oa_package/53/2e/PMC4928349.tar.gz,"[' 3a).', ' 3b).', ' 3c).', 'Plasma phospholipid and CSF DHA at 18 months.', 'A 42 amyloid- 42, CSF cerebrospinal fluid, DHA docosahexaenoic acidAssociation of CSF A 42 with the change in CSF DHA.']","Fig. 3 Plasma phospholipid and CSF DHA at 18months. The distribution of phospholipid and CSF DHA measurements at 18months by baseline CSF A42 tertiles in participants allocated to DHA ( =29) is illustrated. There was no significant difference in plasma phospholipid DHA by A42 tertiles after 18months of DHA. and After 18months of DHA supplementation, there was a significant decrease in CSF DHA levels inparticipants in the the first A42 tertile ( =0.01 for three-way group comparison, =0.01 for difference between T1 and T2, and =0.007 for difference between T1 and T3) and a significantly lower increase in the CSF-to-plasma DHA ratio ( =0.05 for three-way group comparison, =0.05 for difference between T1 and T2, and =0.03 for difference between T1 and T3). The groups were compared using a linear regression model. * <0.05 for group comparison. amyloid-42, cerebrospinal fluid, docosahexaenoic acid",yes
PMC4660376,Figure_1,oa_package/4c/1a/PMC4660376.tar.gz,[],"Fig.1 Axial ( ), coronal ( ) and sagittal ( ) MR images corresponding to a 35-year-old male with a left-sided motor weakness since the age of six months with symptomatic therapy-resistant epilepsy with right-sided lobar hemi-microencephaly of the frontal lobe and insular region, in addition to polymicrogyric pachygyria. The only ipsilateral median nerve (MN) result in the patient group was found in this patient, who also had ipsilateral activation after posterior tibial nerve (PTN) stimulation and for both hand and foot motor responses. : results of MN and PTN stimulation on both sides showing ipsilateral MN and PTN responses. : task-related power decreases in the beta band during self-paced hand and foot movements with localization in the ipsilateral hemisphere for both hand and foot movements. L: left; R: right; A: anterior; P: posterior.",yes
PMC5651405,Figure_1,oa_package/fa/ea/PMC5651405.tar.gz,"['However, a small yellowish granular lesion approximately 10 mm in diameter was noted near the esophageal orifice (), and a biopsy was carried out.', 'Endoscopic findings of esophageal xanthoma.']",Figure 1 Endoscopic findings of esophageal xanthoma. A small yellowish granular lesion is noted in the esophageal mucosa,yes
PMC3436868,Figure_7,oa_package/70/78/PMC3436868.tar.gz,"['This demonstrated that QC is predominantly expressed in neurons in different brain areas (A).', 'g007Ca2+ induced QC and c-fos mRNA expression is neuronal cell type specific.', 'QC is not upregulated by TG treatment in either cell line (B,C).', 'In the Hela cells the response is much lower than in the SK-N-SH cells and in 293 cells TG does not elicit any effect on the c-fos levels (B,C).']",10.1371/journal.pone.0044674.g007,yes
PMC11373119,Figure_1,oa_package/34/d9/PMC11373119.tar.gz,"['1).', '', 'C in situ hybridization of the Epstein-Barr virus (EBER) showing an intense and diffuse nuclear staining in all atypical cellsGiven these findings a full body PET-scan was performed in which aside from the splenic tumor a parasternal adenopathy was found.']","Fig.1 Small magnification (2x) displays the entire width of the pseudocysts capsule. The fibrin deposit can be observed on the top half of the image. H&E stain 40showing groups of large atypical lymphoid cells, high nucleus-cytoplasm ratio, irregular nuclear contour, dense chromatin and clear cytoplasm. in situ hybridization of the Epstein-Barr virus (EBER) showing an intense and diffuse nuclear staining in all atypical cells",yes
PMC8743867,Figure_2,oa_package/38/5f/PMC8743867.tar.gz,"['A repeat coronary angiogram was performed through the right RA, showing 70% stenosis of the proximal LAD and 60% stenosis midvessel ( 2).', ' 2Second Cardiac Catherization in August 2020(A) Diffuse coronary disease with 70% disease of the proximal left anterior descending artery (white arrow), (B) 90% ostial stenosis of the second diagonal, and 80% stenosis of the first marginal branch (black arrow).']",Figure1 First Cardiac Catherization in July 2020 Multivessel coronary disease with 80% stenosis in the right coronary artery and 80% stenosis in the marginal branch . The lesion on the left anterior descending artery had 60% stenosis. Also seen is a lesion of the mid left anterior descending artery .,yes
PMC9479568,Figure_6,oa_package/c7/e7/PMC9479568.tar.gz,"[', rootlet stagnation sign ) [c].', ':Illustrated images of the morphological changes in cervical nerve rootlets in cervical radiculopathy.']","Figure 6: Illustrated images of the morphological changes in cervical nerve rootlets in cervical radiculopathy. (a) The sagittal image shows posterior deviation of the ventral rootlet due to anterior compression. (b) The coronal image shows the ventral rootlet coursing laterally, near the entrance of the intervertebral foramen. (c) The axial image shows the ventral and dorsal rootlets coming close together due to anterior compression at the proximal margin of the compression in the dural canal, and the rootlets appear to be stagnating near the entrance of the intervertebral foramen (rootlet stagnation sign). VR: Ventral root, DR: Dorsal root, and DH: Disk herniation.",yes
PMC6120556,Figure_2,oa_package/bd/3e/PMC6120556.tar.gz,[' 133p53 is increased in glioblastoma with wild type TP53.'],"Figure 2 is increased in glioblastoma with wildtype . (A) Radar plot depicting the frequency of mutations in ALT, TEL, and TELM glioblastoma subtypes. (B) (left) and (right) expression comparison between tumors with wildtype and those with mutations. (C) Heat map and rank hierarchical clustering of the 89 tumors clustered by mRNA expression of , , , , , immune marker CD163, and the telomere maintenance mechanism (TMM) subtype showed five distinct clusters (named groups AE). TMM: dark green = ALTpositive tumor; light green = TELpositive tumor; orange = TELMpositive tumor. (D) Expression of a wellknown p53induced gene, , ( ) amongst the five subgroups identified from the hierarchical clustering. * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001.",yes
PMC3179730,Figure_1,oa_package/90/34/PMC3179730.tar.gz,['Intra-articular IL-1 over-expression in the adult Col1-IL1 XAT transgenic mouse results in joint pathology with behavioral changes.'],"Figure 1 . ( ) Intra-articular injection of FIV(gfp) in Col1-IL1 transgenic ( ) mice (10 L containing a total of 10 infectious particles) had no effect on IL-1 expression in the joints. In contrast, ( ) intra-articular injection of FIV( ) in age matched transgenic mice (10 L containing a total of 10 infectious particles) induced the expression of human IL-1 as detected by immunohistochemistry employing an antibody raised against the mature form of human IL-1. Moreover, ( ) cells infected by FIV(Cre) vector were detected by immunofluorescence (red) utilizing a Texas-Red conjugated antibody raised against the V5 epitope that tagged Cre recombinase in the FIV(Cre) vector (red fluorescence). The reporter gene -galactosidase (the second ORF in the bicistronic Col1-IL1 transgene) was detected by a polyclonal antibody coupled to Alexa Fluor 488 (green fluorescence). Therefore, cells infected by FIV(Cre) appear red and cells expressing -galactosidase appear yellow due to the overlap of green+red. ( ) Col1-IL1 transgenic ( ) mice injected with the control vector FIV( ) (10 L containing a total of 10 infectious particles) did not develop any articular pathology. ( ) Conversely, Tg mice injected with FIV(Cre) intra-articularly developed joint pathology, ( ) characterized by chondrocyte cloning, erosions and fibrillations. ( ) Joint pathology was assessed on histology sections by a 0 - 5 scale. It was found that the mice that received FIV(Cre) intraarticularly ( ) were characterized by a significant degree of joint pathology. ( ) Articular cloning was employed as an additional measure of arthritis, whereby mice with intra-articular FIV(Cre) injection ( ) were characterized by a significantly higher number of cloned chondrocytes in the articular cartilage. Furthermore, mice with arthritis displayed significantly decreased rotarod activity ( ), employed here as a measure of joint dysfunction, as well as ( ) significantly increased body grooming, as a measure of discomfort. *p < 0.05; **p < 0.01; ***p < 0.0001; Bar = 100 M.",yes
PMC4108737,Figure_2,oa_package/b4/98/PMC4108737.tar.gz,[],Figure 2 Aneurysm of anterior connective artery complex,yes
PMC11306801,Figure_5,oa_package/8a/1a/PMC11306801.tar.gz,['The introduction of the sentinel lymph node biopsy as the standard of care for several cancers and the ability of a handheld gamma-detecting probe (HGDP) in direct contact with the tissue led to the development of a wide range of probes and control units ().'],FIGURE 5 Intraoperative Gamma Detection Probes. Three commercially available Bluetooth-enabled low-energy handheld gamma-detecting probes (HGDPs) and a laparoscopic gamma-detecting probe and trocar. Three commercially available high-energy HGDPs with prominent collimators and a drawing of a proposed drop-in gamma detecting probe for robotic minimally invasive surgery (MIS). Newly developed 2nd generation gamma detection probe for detecting high- and low-energy radionuclides and with electronic collimation.,yes
PMC6153806,Figure_3,oa_package/22/08/PMC6153806.tar.gz,"['13) (A and B).', 'Exposure to benznidazole in the absence of infection did not cause myocarditis (A).', 'Baseline levels in noninfected control mice displayed considerable uniformity, with no significant differences between benznidazole-treated and nontreated animals (C and D).', 'However, within this group comparison, approximately half of the JR-infected mice exhibited no evidence of fibrosis, whereas the other half displayed major increases in collagen content (C).', '03) and not significantly different from that in noninfected mice (C and D).', 'Levels were the same as those in the noninfected controls, a pattern similar to that in the JR-C3H combination (C).', 'As expected, we found extensive differences in the level of collagen deposition in the heart muscle of infected nontreated mice (C and D).', 'In the case of myocarditis, there was not a significant association with infection in the JR-C3H model ().']","FIG 3 Curative benznidazole treatment of C3H/HeN mice infected with strain JR prevents the development of cardiac fibrosis. Infected mice (see Materials and Methods) were either nontreated (NT; = 24), or treated (T) with benznidazole for 20 days, as outlined in the legend to , commencing day 14 postinfection ( = 10), or day 73 ( = 12). Groups of noninfected mice, either nontreated (NT; = 16), or treated (T) (starting on day 14, = 8, or on day 73, = 8) were run in parallel. Cardiac tissue was harvested (day 150 to 153) and assessed for inflammation and fibrosis (see Materials and Methods). (A) Quantitative histopathological analysis of inflammation. The number of nuclei per 6 10 m was quantified as a cellular myocarditis index. Data are shown as mean standard deviation. Inflammation in nontreated infected mice was slightly but significantly greater than that in noninfected controls or in drug cured mice. (B) Representative myocardial sections stained with H&E and used to assess inflammation. (C) Quantification of collagen content (blue area in Masson's trichrome-stained sections) as a marker of cardiac fibrosis (see the text for more details and Materials and Methods for description of statistical procedures). (D) Masson's trichrome-stained photomicrographs demonstrating fibrosis in mice heart sections. In panels B and D, the magnification is 400. Bar, 30 m.",yes
PMC3172983,Figure_6,oa_package/46/32/PMC3172983.tar.gz,"['005""/>Cyst with keratinized squamous epithelium and adnexal structures in wall.']",Figure 6 Cyst with keratinized squamous epithelium and adnexal structures in wall.,yes
PMC6892966,Figure_2,oa_package/9f/0d/PMC6892966.tar.gz,"['As shown in A, PBMCs of patients with active TB disease contained significantly elevated percentages of LDNs compared with the PBMCs of control H.', 'B shows a representative analysis of surface markers expression in terms of Geo-MFI in one patient with active TB.', 'As shown in C, both neutrophil populations expressed the myeloid surface markers CD33, CD66b, CD11b, CD10, CD15, CD13, CD16, and HLA-DR, but LDNs showed significantly elevated expression of CD66b, CD33, CD15, and CD16 cell surface molecules, when compared to NDNs population.', 'Frequency and surface molecules expression on neutrophil subsets population.']","Figure 2 Frequency and surface molecules expression on neutrophil subsets population. Box and whisker plots representing cumulative data of LDNs frequencies in active TB patients compared to H.D. Horizontal bars indicate the median, min, and max frequency of each group. Mann Whitney test was used to assess statistically significant differences between the groups, < 0.01. Representative FACS analysis of surface markers expression on circulating neutrophil subsets of one representative active TB patients. Geometric mean fluorescence intensity (Geo-mean) of different surface molecules expressed on LDNs and NDNs of active TB patients ( = 22). Data are expressed as geo-mean SE. Student's test was used to assess statistically significant differences between NDNs and LDNs. < 0.05, < 0.01, < 0.0001. Red histogram represents the Geo-mean of the isotype mAb control. Blue histogram represents the Geo-mean for each myeloid marker expression evaluated with each moAb used.",yes
PMC9719404,Figure_2,oa_package/0b/d9/PMC9719404.tar.gz,"['The numeric results for 1st, 2nd, and 3rd retrobulbar points on the left eye were 5.']","Figure 2 The numeric results for 1st, 2nd, and 3rd retrobulbar points on the left eye were 5.11 mm, 4.88 mm, and 4.70 mm, respectively which were significantly higher when compared with those contralateral glaucomatous eyes This illustration shows a 62-year-old man with bilateral but asymmetric glaucomatous optic nerve damage. The axial diameter of the retrobulbar optic nerve-sheath complex in the middle portion of this patient was 2.90 mm on the right (cup:disc ratio = 0.6) and 2.50 mm on the left (cup:disc ratio = 1.0, total optic atrophy). These measured values for both eyes were clearly thinner than the corresponding mean data of normal control subjects found in this study (mean = 4.15 mm; range 3.50-5.00 mm) and than those of normative values studied before (mean = 4.40 mm; range, 3.20-5.60 mm).",yes
PMC11452191,Figure_3,oa_package/60/7c/PMC11452191.tar.gz,"['We further examined morphological changes in sensory nerves and blood vessels in DIO ear skin (Supplementary a, b).', 'Among the three neurotrophic factors tested, we observed increased expression of Ngf in the epidermis of DIO ear skin compared to control, whereas no significant changes were detected in the expression levels of Bdnf and Ntf3 (a).', 'We next addressed whether the onset of NGF expression in the epidermis correlates with the development of sensory hypersensitivity in DIO mice (b).', 'At 22 weeks-of-age, X-gal staining of ear skin sections from NGF-LacZ mice showed increased expression of NGF in the epidermis of DIO ear skin compared to control (c, d).', 'For quantification measurements, we further validated the enhanced NGF expression in the epidermis of DIO ear skin using a fluorescent version of LacZ detection, SPiDER- gal staining in conjunction with the keratinocyte markers K10 and K14 (e r).', 'Considering that keratinocytes constitute over 95% of the cells in the epidermis, we initially isolated keratinocytes from ear skin of adult mice, cultured them with glucose, insulin, or a combination of both, and subsequently assessed Ngf expression (t, u).', 'A high level of insulin enhances Ngf expression in primary keratinocytes in culture, whereas a high level of glucose did not show any inductive effect on its expression (u).', '603316v1-f0002"" position=""float""/>.']","Fig. 3. DIO enhances NGF expression in the epidermis. ( ) Relative mRNA expression levels of neurotrophic factors ( , , and ) in the epidermis of ear skin from control and DIO mice at 22 weeks-of-age. Expression levels are normalized by those in the epidermis of ear skin from control mice. N=4 in each group. ( ) Schematic diagram of the induction of DIO in mice. ( - ) Representative images of X-gal staining of ear skin sections of control (c) and DIO mouse (d) at 22 weeks-of-age (blue). Scale bars: 100 m. ( - ) Representative double immunofluorescence images of ear skin sections of control (e-g, k-l, and o-p) and DIO mice (h-j, m-n, and q-r) at 22 weeks-of-age. SPiDER-gal (e, h, k, m, o, and q, green; f, i, l, n, p, and r, white) together with antibodies to Tuj1 (e and h, red; g and j, white), K14 (k and m, red), or K10 (o and q, red) were used. Dashed lines (c-d, e-r) indicate the border between the epidermis (e) and the dermis (d). ( ) Total SPiDER-gal fluorescence in the epidermis of ear skin from mice at 18 weeks-of-age (N=4 control, N=4 DIO), 22 weeks-of-age (N=3 control, N=4 DIO), and 30 weeks-of-age (N=4 control, N=3 DIO). See also and . ( ) Schematic diagram of primary mouse keratinocyte culture. Keratinocytes were isolated from adult ear skin and cultured with glucose, insulin, or a combination of both ( ) Relative mRNA expression levels in primary mouse keratinocytes cultured with glucose (+Glu), insulin (+Ins), or a combination of both (+Glu +Ins). Expression levels are normalized by those in primary mouse keratinocytes cultured without glucose and insulin. N=3 in each group. Results are shown as the mean SEM. *p<0.05, **p<0.01; NS, not significant (p > 0.05). values were determined by the parametric two-tailed test. The schematic diagrams were partially created with .",yes
PMC8085788,Figure_2,oa_package/8d/05/PMC8085788.tar.gz,"['Images one year prior and during the course of the admission while in the Covid-19 pandemia shown in demostrate persistent and stable bilateral apical thickening, parenchymal fibrosis, upper lobe volume loss and traction bronchiectasis (', 'Coronal (A and B) and axial (C) unenhanced CT of the chest demonstrating biapical pleural thickening and subpleural fibrosis with elevation of the right hilum (A).', 'DiscussionIdiopathic interstitial pneumonia classification was revised in 2013 and two rare interstitial pneumonias were recognized; idiopathic lymphoid interstitial pneumonia and PPFE (Table 1) [1].']",Fig. 2 Coronal (A and B) and axial (C) unenhanced CT of the chest demonstrating biapical pleural thickening and subpleural fibrosis with elevation of the right hilum (A). Comparison of 1-year prior unenhanced CT in coronal (D and E) and axial (F) planes demonstrate grossly stable findings of PPFE.,yes
PMC9577043,Figure_9,oa_package/b8/05/PMC9577043.tar.gz,[],Fig. A.5 A few example mosaic images build of a balanced set of representative background and foreground (lymph node tissue region) tiles of whole slide images from one scanner to normalize the data of other scanners.,yes
PMC4456805,Figure_5,oa_package/54/ee/PMC4456805.tar.gz,"[' 5a).', ' 5b and c).', ' 5d).', ' 5e-g).', 'E2f8 regulates fabp3 expression in zebrafish and human cells.', 'controlCo-administration of anserine and creatine suppressed obesity-associated phenotypes via downregulation of fabp3To confirm that fabp3 expression is responsible for the improvement of hepatic steatosis, we administered anserine and creatine to DIO-zebrafish as anti-obesity therapeutics.']","Fig. 5 E2f8 regulates expression in zebrafish and human cells. ( ) qPCR analysis of in zebrafish analysed in Fig. . expression was suppressed by MO-e2f8 i.p. n=5, * <0.05 vs. control MO (MO-con). ( ) qPCR analysis of forced expression of ( ) and ( ) in HepG2 cells. n=4, ** <0.01 vs. control. ( ) FABP family gene expression in E2F3-or E2F8-overexpressed (OE) cells. FABP3 were induced only in E2F8-OE cells ( ), while FABP1 ( ), FABP5 ( ) and FABP7 ( ) were not altered in these OE cell lines. n=4, * <0.05 vs. control",yes
PMC9325972,Figure_7,oa_package/f6/58/PMC9325972.tar.gz,[],Figure7 Summary of the ex vivo effects of extracellular vesicles from hyperammonemic rats on cerebellar slices from control rats.,yes
PMC5171776,Figure_3,oa_package/8f/e0/PMC5171776.tar.gz,"['Bisulfide sequencing was performed on control and acrolein treated bladder muscle cells with a focus on the specific CpG islands within the Ogg1 gene promoter and exon1 from 800 bp to +336 bp, including 51 CpG sites (A).', 'What we termed as regions III and IV had a five-fold increase acrolein-induced CpG methylation (A).', 'In examining the loading of the proteins responsible for the observed methylation, DNA methyl transferase (Dnmt1 and Dnmt3b) binding to Ogg1 Region III was greater when treated with acrolein compared to control (B,C).', 'helped prepare and 4.', 'org/1999/xlink"" xlink:href=""srep39257-f2""/>DNA methylation analysis of the Ogg1 gene.']","Figure 3 DNA methylation analysis of the Ogg1 gene. ( ) Bisulfite sequencing was performed on the gene from promoters region (800bp downstream of transcription start site) to exon1 (+336bp up to transcription start site) from cultured mouse bladder detrusor cells treated with vehicle or acrolein. The CpG islands sequenced fall into regions I, II, III, and IV. The open circles represent the unmethylated cytosine, whereas filled circles represent the methylated cytosine. ( ) ChIP analysis targeting DNA methylation binding site within the region III (41bp to 103bp). Dnmt1, Dnmt3A, Dnmt3B, and RNA polymerase II loading onto region III was compared the control and acrolein conditions. Non-immunoprecipitated chromatin was used as total input control. PCR of input DNA shows equivalent starting material for the assay. Normal rabbit IgG antibodies served as the negative control. ( ) Three independent ChIP assays on region III of the promoter were analyzed by quantitative PCR for the occupancy of Dnmt1, Dnmt3a, Dnmt3b and Pol II.",yes
PMC11096686,Figure_7,oa_package/b9/be/PMC11096686.tar.gz,"['A sample prediction for all five properties is shown in ', '']",Fig.7 Predictions made by the AIPS-N model. The AIPS-N model predicted the AIPS-N score of five properties for a CT slice embodying a lung cancer nodule. The AIPS-N scores are used to train the AIPS-M ML and DL models.,yes
PMC5707734,Figure_4,oa_package/1f/42/PMC5707734.tar.gz,"['CaCo-2 cells were transiently transfected with TCF-wt GFP (A).', 'Thanks to the GFP reporter construct, the small percentage of WNT active cells can be easily followed (A).', 'Upon treatment with AAE or LAE (400 mg/L, 48 h), GFP expression decreased in CaCo-2 cells (A,B), indicating that the extracts efficiently inhibited the WNT pathway in these cells.', '0 g/L, (B).', 'AAE and LAE act as WNT inhibitors in CaCo-2 cells.']",Figure 4 AAE and LAE act as WNT inhibitors in CaCo-2 cells. ( ) Activity of the WNT reporter construct TCF-wt GFP in CaCo-2 cells cultivated for 48 h in the presence or in the absence of LAE (400 mg/L) (representative of five experiments) (BF = Bright Field; scale bar is shown); ( ) WNT pathway activity (green bars) and cell viability (magenta bars) of CaCo-2 cells transfected with TCF-wt GFP and cultivated in the absence () or in the presence of the indicated concentration of LAE (or of the corresponding dilution of DMSO). Values on the left axes indicate changes in GFP expression (a.u.). Values on the right axes indicate changes in cell viability expressed as percentage of untreated cells ().,yes
PMC11268842,Figure_3,oa_package/1b/01/PMC11268842.tar.gz,"['7 cm2 and transmitral mean gradient of 13 mmHg, Mild Mitral Regurgitation, Mild Aortic Regurgitation and Moderate Pulmonary hypertension with no left atrial thrombus ().', 'Pre-procedure transesophageal echocardiography.']",Figure 3 Pre-procedure transesophageal echocardiography.,yes
PMC2684428,Figure_4,oa_package/a1/71/PMC2684428.tar.gz,['Microscopic examination revealed a dermal tumor composed of epithelial cell islands surrounded by lakes of mucin consistent with the diagnosis of mucinous carcinoma [].'],Figure 4 Histopathology: H and E (100) (a) Epidermis (b) Dermal adnexa (c) Mucin matrix (d) carcinoma cells. Inset (e) 400 view of carcinoma cells within mucin matrix,yes
PMC11404707,Figure_2,oa_package/4d/fd/PMC11404707.tar.gz,"[', 2023) ().', '.']","Fig. 2. H MRSI of the whole brain. Reconstructed sagittal and coronal T -weighted MPRAGE images from a single subject. The 280 280 135 mm (left-right x anterior-posterior x inferior-superior) field-of-view is superimposed (dotted green lines), along with the 280 280 100 mm excitation slab (solid yellow lines). Four reconstructed axial T -weighted MPRAGE images ( ) and their indicated positions along the sagittal plane (blue arrows). Shown in ( ) is the water reference image, used for image registration, B - and eddy-current correction, and signal normalization; ( ) are the segmented tissue maps of cerebrospinal fluid (CSF), gray matter (GM), and white matter (WM), down-sampled to the MRSI resolution; ( ) are the metabolite maps of choline (Cho), creatine (Cr), glutamate-plus-glutamine (Glx), -inositol (mI), and -acetyl-aspartate (NAA); and ( ) is the quality map, in which red indicates voxels that fulfilled all quality criteria, including metabolite linewidth <12 Hz and signal <3 standard deviations from the mean.",yes
PMC6425074,Figure_2,oa_package/4c/46/PMC6425074.tar.gz,[],Fig. (2) Mechanisms of peroxisome proliferator-activated receptor gamma (PPAR) transrepression- Co repressor interference mechanism- co-repressor complexes occupied on the promoters region of NF-kB is stabilize by PPAR agonist by SUMOylation of PPAR ligand binding domain within PPAR and inhibit proinflammatory inhibitors Cross coupling- direct binding of PPAR to NF-kB forming inactive transcriptional complexes and inhibit proinflammatory inhibitors. ( ).,yes
PMC10394653,Figure_1,oa_package/dc/7e/PMC10394653.tar.gz,[],"Figure1 Scatterplots of potential effects of SNPs on Gut microbiota versus Asthma and its phenotypes Barnesiella; CandidatusSoleaferrea; RuminococcaceaeUCG014(Asthma); Akkermansia; Collinsella; RuminococcaceaeUCG014(Childhood-onset asthma); FamilyXIIIAD3011group; Eisenbergiella; Ruminiclostridium6. Scatter plots presented the per-allele association with outcome risk plotted against the per-allele association with one standard deviation of exposure (with vertical and horizontal purple lines showing the 95% CI for each SNP). Analyses were conducted using the Inverse Variance Weighting (IVW), Weighted median, Wald ratio, Robust Adjusted Profile Score (RAPS), MR Egger, MR-PRESSO, and Maximum likelihood (ML) methods. The slope of each line corresponding to the estimated MR effect per method. CI, confidence interval; SNP, single-nucleotide polymorphism; MR-PRESSO, Mendelian Randomization Pleiotropy RESidual Sum and Outlier.",yes
PMC4910366,Figure_3,oa_package/ea/d9/PMC4910366.tar.gz,['Scoring criteria of DWI.'],"Figure 3 Scoring criteria of DWI. (a and b) Score 1: no reduction in ADC compared with normal glandular tissue. No increase in SI on any high b-value image (b 800); (c and d) score 2: Diffuse, hyper SI on b 800 image with low ADC; no focal features. However, linear, triangular, or geographical features are allowed; (e and f) score 3: intermediate appearances not in categories 1/2 or 4/5; (g and h) score 4: focal area(s) of reduced ADC, but iso-intense SI on high b-value images (b 800); (i and j) score 5: focal area/mass of hyper SI on the high b-value images (b 800) with reduced ADC. DWI: Diffusion-weighted imaging; ADC: Apparent diffusion coefficient; SI: Signal intensity.",yes
PMC10525531,Figure_7,oa_package/b0/2c/PMC10525531.tar.gz,['A schematic showing hepcidin involvement in CAG injury.'],"Figure 7 A schematic showing hepcidin involvement in CAG injury. CAG can increase IL-6 expression and then activate hepcidin via the JAK2/STAT3 signaling pathway. On the one hand, high levels of hepcidin restrain FPN1, causing iron overload and ferroptosis in CAG gastric tissue, as indicated by a higher level of 4-HNE and a lower level of GPX4. On the other hand, hepcidin is expressed and released by parietal cells and flows into the duodenum. Subsequently, high levels of hepcidin inhibit FPN1 expression in the basal membrane of epithelial cells and DMT1 expression in the brush border membrane of the duodenum, resulting in iron uptake deficiency in the duodenum.",yes
PMC9569638,Figure_4,oa_package/ee/e1/PMC9569638.tar.gz,"['A shows the Volcano plot graph of the differentially expressed microRNAs comparing the platelets derived from the blood of pancreatic cancer and healthy subjects.', 'Interestingly, we found that a Principal Component Analysis (PCA) showed a clear separation of platelets microRNA expression between the blood of the cancer samples (blue circles) and those of the cancer, in which two groups are apparent: microRNAs derived from blood platelets (red circles) and those derived from pancreatic juice (green circles) (B).', 'The expression data were then subjected to an unsupervised hierarchical clustering resulting in a heatmap where the samples belonging to the pancreatic cancer patients (both from blood and pancreatic drainage) were clustered together against the control group (C).', 'control in each case (D top and middle panel).', 'Interestingly, when we compared the microRNA expression of platelets obtained from cancer patients blood versus those obtained from pancreatic juice, we found that they differed by clustering separately (D bottom panel).', 'We analyzed the expression of four of these microRNAs in platelets co-cultured with pancreatic cancer cells (E).', 'Differentially expressed miRNAs in platelets derived from pancreatic cancer patients (Tumor Educated Platelets).']","Figure 4 A total of 4 blood samples and 4 pancreatic juice samples were taken from 4 different patients of pancreatic cancer. These samples were paired with 4 blood samples from healthy donors. ( ) Volcano plot that shows statistical ( value) versus magnitude of change (fold change) of differentially expressed platelet transcriptome of patients with tumor cancer and of people without cancer; ( ) Principal component analysis of platelets transcriptome of patients with tumor cancer and of people without cancer; ( ) Unsupervised hierarchical clustering of all samples. Two groups were found that discriminates between pancreatic cancer and control individuals: ( ) Clustering heatmap of pancreatic drainage samples vs. control blood. Top panel: Two groups were found by k-means clustering algorithm. Heatmap of blood samples cancer vs. control by k-means clustering middle panel. Heat map of platelets from blood (Bp) or platelets of pancreatic juice (Pjp) cancer; ( ) Result of the machine-learning signal-noise analysis between cancer samples, in this case we selected the top miRNAs to 8 candidates with an optimal -value; ( ) Top 4 differentially expressed miRNAs quantification by qPCR from platelets trained with direct contact with the BxPC-3 cells against control platelets (* < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001).",yes
PMC10591055,Figure_7,oa_package/7f/d9/PMC10591055.tar.gz,"['3,4 After 2 weeks, the xenograft-implanted mice were randomly divided into four groups and intraperitoneally injected with (1) PBS vehicle, (2) cisplatin (1 mg/kg) alone, (3) GSH inhibitor (BPTES 5 mg/kg, BCNU 5 mg/kg, or BSO 2 mg/kg, or (4) cisplatin plus GSH inhibitor via 10 injection cycles at 3 day intervals, except for daily injection of BSO ( 7A).', '76% in the cisplatin monotherapy group (s 7B and 7C).', '52%) ( 7C).', ' 7GSH interference resensitizes cisplatin-resistant human MIBC cells(A) Overview of the orthotopic BC xenograft model for evaluating the efficacy of monotherapy with cisplatin (1 mg/kg), BPTES (5 mg/kg), BCNU (5 mg/kg), or BSO (2 mg/kg) and that of combination therapy with cisplatin and these GSH dynamics inhibitors.', 'Histological analysis revealed that cotreatment with cisplatin and GSH inhibitors significantly suppressed tumor growth in the bladders, and the inhibitory effect was not detected in animals treated with vehicle, cisplatin monotherapy, or GSH inhibitor monotherapy (s 7D and S7D).', 'Immunofluorescent staining revealed that xenograft tumors with vehicle treatment exhibited higher levels of GLS, GSR, and GCLM proteins relative to bladder tissues in the sham group, and cisplatin alone further increased these proteins in xenograft tumors relative to the vehicle-treatment group (s 7E and S7E).', 'Consistent with the findings of the in vitro cell model assays, cotreatment with GSH inhibitors and cisplatin efficiently repressed expression of BC stem cell markers, including CD44v6 and KRT14 ( 7E).', 'Specifically, some cancer cells with high levels of CD44v6 protein coexpressed GLS protein in xenograft tumors from animals treated with cisplatin alone or vehicle, and the frequency of the CD44v6+/GLS+ coexpressing cells was significantly decreased by combination treatment with both cisplatin + BPTES and cisplatin + BSO ( 7F).']","Figure6 GSH dynamics modulate the chemoresistance of human MIBC cells (AD) Cell viability assays following exposure to the indicated concentrations of cisplatin in Cis_NR T24 cells with gene silencing (A and C) or chemical inhibition (D)of the GSH dynamics modulators as well as in naive T24 human MIBC cells (B)ectopically expressing the indicated genes. NT, nontreated control. (EG) Apoptosis assays based on flow cytometry (E and F) with Annexin-V/PI staining (E)and immunoblotting (G)of cleaved caspase-3 and PARP in Cis_NR T24 cells treated with a suboptimal cisplatin concentration (2g/mL) and the indicated GSH dynamics inhibitors for 24 h. (F) Quantification of apoptotic cells (% of Annexin-V /PI and Annexin-V /PI population). (H and I) Cell viability (H)and apoptosis (I)assays in Gem_NR KU-19-19 cells treated with gemcitabine (20M) and the indicated GSH dynamics inhibitors. NT, nontreated control. Quantitative data are expressed as the mean SEM.",yes
PMC2832341,Figure_3,oa_package/5d/df/PMC2832341.tar.gz,['.'],Figure 3. Heparanase expression in the diagnosis of broncopulmonar carcinoid tumors. Optical microscopy at X400 power: ) negative expression of heparanase (absence of stainingperoxidasein cells cytoplasm) in bronchial mucosa not compromised by neoplasm; ) positive expression of heparanase (presence of cytoplasm full of peroxidasebrownish areas) in broncopulmonar carcinoid tumor. (Adapted from: de Matos et al ).,yes
PMC5370253,Figure_5,oa_package/36/bd/PMC5370253.tar.gz,"['4) Distance from the PSAA to the zygomatic arch (): considering the extent of the zygomatic arch, the most anterior and inferior point of the zygomatic arch was marked first, and then the closest distance from this point to the PSAA was measured using the 3-dimensional distance measurement feature of the CBCT software.', '5) Distance from the PSAA to the nasal septum (): the closest distance from the PSAA to the nasal septum was measured.', 'Distance from the artery to the zygomatic arch (A) and to the nasal septum (B).']",Fig. 5 Distance from the artery to the zygomatic arch (A) and to the nasal septum (B).,yes
PMC10314239,Figure_3,oa_package/07/6d/PMC10314239.tar.gz,"['MRI of the abdomen: (A) coronal T2W; (B) sagittal T2W; (C D) axial T2W; (E) T2W fat saturation (fat sat); (F) T1W fat sat; (G) DWI and (H) ADC images: showing an enlarged transplanted kidney in the right iliac fossa with linear cortical and peri-pyramidal high T2 signal (green arrows); septated peri pelvicalyceal cystic structures (white arrows); septated perinephric fluid (orange arrows) which appear hypointense on T1W fat sat (black arrow), with traces of fluid in the abdominal wall (yellow arrows); mild free fluid in the upper abdomen and pelvis (blue arrows) and no diffusion restriction (brown arrows).']","Figure 3 MRI of the abdomen: (A) coronal T2W; (B) sagittal T2W; (C & D) axial T2W; (E) T2W fat saturation (fat sat); (F) T1W fat sat; (G) DWI and (H) ADC images: showing an enlarged transplanted kidney in the right iliac fossa with linear cortical and peri-pyramidal high T2 signal (green arrows); septated peri pelvicalyceal cystic structures (white arrows); septated perinephric fluid (orange arrows) which appear hypointense on T1W fat sat (black arrow), with traces of fluid in the abdominal wall (yellow arrows); mild free fluid in the upper abdomen and pelvis (blue arrows) and no diffusion restriction (brown arrows). Native kidneys are atrophic (red arrows). T2W: T2-weighted image; T1W: T1-weighted image; DWI:diffusion weighted imaging; ADC:apparent diffusion coefficient",yes
PMC10204786,Figure_3,oa_package/03/78/PMC10204786.tar.gz,[],,yes
PMC9705270,Figure_4,oa_package/c3/f5/PMC9705270.tar.gz,"['The correct orientation of the osteotome has a medial to lateral obliquity so that the lateral portion can slide under the medial portion when unlocking the ramus ().', 'Pelvic models showing correct orientation of superior pubic ramus osteotomy (A) and incorrect orientation (B).']",Fig 4 Pelvic models showing correct orientation of superior pubic ramus osteotomy (A) and incorrect orientation (B). The osteotomy should be performed from the superomedial to inferolateral aspect of the superior pubic ramus to allow for free motion of the articular block. The arrows are directed from inferior to superior.,yes
PMC8356707,Figure_3,oa_package/57/62/PMC8356707.tar.gz,['Few of the major discordances observed in this study are illustrated in .'],"Figure 3 Photomicrograph of few discordant cases. (a) Scanty focus of metastatic carcinoma in lymph node, (b) focal dysplasia at esophageal cut margin, (c) scanty focus of granuloma in lymph node, (d) foci of intra mucosal adenocarcinoma in serrated adenoma which were missed on whole slide imaging diagnosis",yes
PMC4909602,Figure_2,oa_package/2f/14/PMC4909602.tar.gz,"['4 mg/dL, and MR imaging reported bilateral high grade (2 3) hydronephrosis with enlarged pelvic lymph nodes and multiple bone metastases(', 'The histopathology of gastroscopic reported undifferentiated prostate cancer metastasis(', 'Immunohistochemically, the neoplastic cells showed positive stains for alpha methylacyl coenzyme A-reductase (AMACR) (', ' 2a.']","Figure2 a. MR imaging revealing bilateral hydronephrosis of kidney. b. Endoscopic scene of suspicious area in gastric fundus. c. Diffuse AMACR positivity is seen in nearly all neoplastic cells that obtained from gastric biopsy (immunoperoxidase,40).",yes
PMC4220412,Figure_1,oa_package/10/88/PMC4220412.tar.gz,"['Ten eyes (10 patients) were identified [].', 'Five eyes had sutures in-situ at the time of recurrent MK [].', 'At the latest episode of MK, 9 had a BCL, 7 were using topical steroids, 7 were using topical lubricants, 4 had concurrent meibomian gland dysfunction and were taking oral Doxycycline, 8 had occluded eyelid puncti and 5 had sutures at the time of recurrent infection [Table 2 and ].', 'Montage of eyes in this study.']","Figure 1 Montage of eyes in this study. (a) Traumatic lid loss and several eyelid procedures including medial and lateral tarsorraphy showing active microbial keratitis. (b) Keratoconjunctivitis sicca secondary torheumatoid arthritis, a corneal patch graft and active microbial keratitis. (c) Keratoconjunctivitis sicca secondary to icthyosis, tarsorraphies, corneal patch graft and active microbial keratitis. (d) Keratoconjunctivitis sicca secondary to rheumatoid arthritis, lateral tarsorraphy, corneal patch graft and active microbial keratitis (e) Cicatricial phemphigoid, previous corneal graft and active microbial keratitis (f) Patient 6 with atopy and history of herpetic keratitis, corneal graft with active keratitis (g) Corneal graft for PBK and active microbial keratitis. (h) Steven Johnson Syndrome and Graft Vs Host Disease with previous eyelid procedures showing active microbial keratitis.(i) Keratoconjunctivitis sicca secondary to rheumatoid arthritis, previous tarsorraphy and microbial keratitis with drug deposits.(j) Corneal graft for pseudophakic bullous keratopathy, keratoconjunctivitis siccas due to rheumatoid arthritis and active microbial keratitis",yes
PMC3447430,Figure_7,oa_package/db/09/PMC3447430.tar.gz,"['The images from three patients showed fluid filled areas around spine and epidural space corresponding to a par-avertebral abscess () and epidural empyemas ().', '.']","Figure 7. ( ) T1-weighted + CM image, sagittal plane. ( ) T1-weighted + CM image, transverse plane. L2-L3 spondylodiscitis. Prevertebral abscess.",yes
PMC9320164,Figure_2,oa_package/1f/2e/PMC9320164.tar.gz,"['The neuroanatomy of the anterior and lateral thoracic wall is shown in .', 'Neuroanatomy of the anterior and lateral thoracic wall [5].']","Figure 2 Neuroanatomy of the anterior and lateral thoracic wall [ ]. Legend: RI, first rib; RII, second rib; RIII, third rib; RIV, fourth rib; RV, fifth rib; RVI, sixth rib; PMa, pectoralis major muscle; SA, serratus anterior muscle; IC, intercostal nerves; TD, thoracodorsal nerve; LT, long thoracic nerve.",yes
PMC1480599,Figure_7,oa_package/6f/f0/PMC1480599.tar.gz,"['g006""/>The expressions of genes from the IFN-signaling pathway were strikingly similar between HCV-infected mice and patients (A and 7B, respectively), indicating that HCV is triggering the same host innate antiviral response pathways in both populations.', 'Induction of IFN-Signaling Pathways in HCV-Infected Mice and PatientsPathway Builder (Protein Lounge) and the Pathway feature of Resolver were used to visualize the expression of genes from the IFN signaling pathway in HCV-infected mice (A) and patients (B).']","Figure 7 Induction of IFN-Signaling Pathways in HCV-Infected Mice and Patients Pathway Builder (Protein Lounge) and the Pathway feature of Resolver were used to visualize the expression of genes from the IFN signaling pathway in HCV-infected mice (A) and patients (B). The expression profiles of individual genes are shown in boxes, and each bar within the box represents one experiment. Color schemes are as indicated in . The reference for each microarray experiment was a pool of normal, uninfected human liver tissue.",yes
PMC9791432,Figure_3,oa_package/65/d0/PMC9791432.tar.gz,"['Emergent MRI confirmed the diagnosis of pseudoaneurysm (figure 3).', 'Axial MRI with contrast of pelvis showing 1.']",Figure 3 Axial MRI with contrast of pelvis showing 1.41.5cm enhancing structure involving the uterine fundus consistent with uterine artery pseudoaneurysm.,yes
PMC7393746,Figure_2,oa_package/79/9e/PMC7393746.tar.gz,"['The final pathology showed foamy histiocyte aggregation and acute and chronic inflammation with numerous Michaelis-Gutmann bodies, consistent with malakoplakia ().', '.']","Fig. 2. (A, B) Foamy epithelioid histiocytes and some lymphocytes in endomyometrium and pelvic cavity (A: hematoxylin and eosin [H&E] stain, 100, B: H&E stain, 200). (C) Histiocytes with basophilic bodies containing calcium (H&E stain, 400). (D) Michaelis-Gutmann bodies noted (Von Kossa stain, 400).",yes
PMC3995393,Figure_3,oa_package/01/3b/PMC3995393.tar.gz,['Photograph of the lesion taken 2 months after treatment.'],Fig. 3 Photograph of the lesion taken 2 months after treatment. Note the scar formation.,yes
PMC6226296,Figure_1,oa_package/62/f9/PMC6226296.tar.gz,"['A, Left wrist dorsal 4 days post surgery (top) and 21 days post surgery (bottom).']","Figure 1 , Left wrist dorsal4 days post surgery (top) and 21 days post surgery (bottom). , Left cubital fossa4 days post surgery (top) and 21 days post surgery (bottom). , Left wrist volar4 days post surgery (top) and 21 days post surgery (bottom).",yes
PMC6812434,Figure_1,oa_package/77/99/PMC6812434.tar.gz,"['HRCT chest with bleomycin pulmonary toxicity showing multiple nodular ground glass areas diffusely involving both the lungs representing mild and early changes of lung injuryHRCT showing classical appearance of lung toxicity as extensive diffuse alveolar damage in bilateral lungs seen as a) Diffuse ground glass opacities and b) Ground glass opacity and areas of basal consolidation c) NSIP patternHRCT changes representing sequel of bleomycin toxicity, a) Fibrotic type of NSIP b) DAD with bronchiectasis and septal thickening c) UIP patternChest X-ray showing nodular and patchy opacities predominantly in lower zonesDISCUSSIONBleomycin is an antitumor antibiotic which was first extracted from the fungus Streptomyces verticillus in 1966.']",Figure 1 HRCT chest with bleomycin pulmonary toxicity showing multiple nodular ground glass areas diffusely involving both the lungs representing mild and early changes of lung injury,yes
PMC4237202,Figure_6,oa_package/51/00/PMC4237202.tar.gz,"['We found that mutating UbS2 of SCA3-causing ataxin-3 reduces its steady-state levels in cultured mammalian cells (figure 6A).', 'Just as we observed with normal ataxin-3, mutating UbS2 enhances the turnover rate of pathogenic ataxin-3 in cells (figure 6B).', 'Also, proteasomal inhibition diminishes the marked difference in protein levels between ataxin-3 versions that have an intact or mutated UbS2 (figure 6C).', 'As shown in western blots in figure 6D, knocking down Rad23 or removing one copy of this gene leads to lower levels of ataxin-3 in Drosophila eyes.', 'When expressed selectively in fly eyes, this ataxin-3 variant does not greatly impact the external eye compared to wild type controls (figure 6E, compare columns I and II).', 'As shown in figure 6E column III, knockdown of Rad23 dramatically suppresses degeneration caused by pathogenic ataxin-3 in fly eyes.', 'When we express pathogenic ataxin-3 on a Rad23 hemizygous background, we again observe suppressed SCA3-dependent retinal degeneration (columns IV and V in figure 6E).', 'Together, data in figure 6 indicate that pathogenic ataxin-3 protein is also regulated by UbS2 and Rad23.', 'Full western blots for images in figures 1 6 are shown in supplementary figure 6.', 'Turnover of pathogenic ataxin-3 protein is also regulated by UbS2A, C) Left: Western blots from HeLa cells transfected as indicated and treated or not with MG132 (4 hours, 15 M) 24 hours after transfection.']","Figure 6 Turnover of pathogenic ataxin-3 protein is also regulated by UbS2 A, C) Left: Western blots from HeLa cells transfected as indicated and treated or not with MG132 (4 hours, 15M) 24 hours after transfection. Right: Means of ataxin-3 signal quantified from blots on the left and other similar experiments. P values are from Student T-tests. Error bars: standard deviations. N of independently conducted experiments is provided in the respective panels. B) Left: Western blots of HeLa cells transfected as indicated and treated with CHX 24 hours later. Right: Means of ataxin-3 signal quantified from blots on the left and other similar experiments. P values are from Student T-tests. Error bars: standard deviations. N=7 independently conducted experiments. D) Left: Western blots from at least 10 dissected fly heads for each indicated group. Right: Means of ataxin-3 signal quantified from blots on the left and other similar experiments. P values are from Student T-tests. Error bars: standard deviations. N=8 independent experiments. Flies were 12 days old. All flies were heterozygous for UAS-ataxin-3, UAS-Rad23-RNAi (where indicated), and the gmr-Gal4 driver. E) External photos and internal sections of fly eyes expressing UAS-ataxin-3(SCA3) in the isogenic background of the UAS-RNAi line targeting Rad23 (2 column), with UAS-RNAi targeting Rad23 (third column), in the isogenic background of Rad23 deficiency (4 column, which is the control for column 5; see main text for description), or in the presence of one copy of Rad23 (deficiency line; 5 column). Driver: gmr-Gal4. Double headed arrows: ommatidial boundaries. Boxes: proteinaceous inclusions that contain ataxin-3(SCA3) . Control in 1 column contained only gmr-Gal4. The other lines were heterozygous for the gmr-Gal4 driver and UAS-transgenes. Images are representative of sections from at least six flies per group and experiments were conducted independently in triplicate with similar results. Flies were 14 days old. Scale bars in histological sections: 50M.",yes
PMC8415640,Figure_4,oa_package/89/79/PMC8415640.tar.gz,"['The MRI revealed moderate T2 lengthening in the bilateral ION, consistent with bilateral HOD ().', '.', '1177_26331055211007445-fig4"" position=""float""/>Case 5A 44-year-old male presented for outpatient follow-up of a previously diagnosed central pontine hemorrhage in 2013 ().']","Figure 4. (a) A 26-year-old male who presented following remote trauma was found to have multiple lesions consistent with a history of high-grade diffuse axonal injury, including lesions best visualized on susceptibility-weighted sequences located in the right dentate nucleus and (b) increased T2 signal in the bilateral inferior olives.",yes
PMC9513936,Figure_2,oa_package/04/bc/PMC9513936.tar.gz,"[' 2C).', ' 2C, E).', ' 2D, F).', 'Dark vs typical microglia s interactions with dystrophic neurites and A plaques.', 'Purple pseudo-coloring = fibrillar A , pink pseudo-coloring = dystrophic neurites, red outline = plasma membrane, yellow outline = nuclear membrane, orange asterisk = mitochondria, green asterisk = altered mitochondria, blue asterisk = endoplasmic reticulum, purple asterisk = dilated endoplasmic reticulum, red arrow = Golgi apparatus, 2nd = secondary lysosome, A = axon terminalDark microglia interact less with parenchymal elements and the vasculature than typical microglia in aged APP-PS1 miceDark microglia contacted significantly more dystrophic neurites than typical microglia in 20-month-old APP-PS1 mice.']","Fig. 2 Dark vs typical microglias interactions with dystrophic neurites and A plaques. Representative 5nm resolution scanning electron microscopy images captured in the ventral hippocampus CA1 of 20-month-old APP-PS1 male mice. Typical microglia (TM) observed near extracellular fibrillar A (pseudocolored in purple) and dystrophic neurites (pseudocolored in pink). Dark microglia (DM) interacting with several dystrophic neurites along with fibrillar A. Quantitative graphs representing the numbers of contacts with dystrophic neurites ( ) and A plaques ( ) as well as the proportion of microglial cells contacting dystrophic neurites ( ) or A plaques ( ). Data are shown as individual dots of either 0 or 100 values and are expressed as meansS.E.M. * p<0.05, ** p<0.01, using a non-parametric MannWhitney test. Statistical tests were performed on n=912 microglia per animal with N=3 mice/group, for a total of 111 microglial cell bodies analyzed. Purple pseudo-coloring=fibrillar A, pink pseudo-coloring=dystrophic neurites, red outline=plasma membrane, yellow outline=nuclear membrane, orange asterisk=mitochondria, green asterisk=altered mitochondria, blue asterisk=endoplasmic reticulum, purple asterisk=dilated endoplasmic reticulum, red arrow=Golgi apparatus, 2nd=secondary lysosome, A=axon terminal",yes
PMC11452235,Figure_2,oa_package/08/91/PMC11452235.tar.gz,"['Endometrium appeared thickened with a suspicion of a large pedunculated mass filling the whole uterine cavity extending from the fundus to the internal cervical os ().', '001"" position=""float""/>Pelvic MRI with gadolinium revealed an anteverted and bulky due to large fibroids within the posterior wall.']","Figure 2 Pelvic MRI with gadolinium revealed an anteverted and bulky due to large fibroids within the posterior wall. Two intramural (FIGO 4) fibroids measuring 4.6 4.0 and 4.5 3.0cm, respectively, were noted. Endometrium appeared thickened with a suspicion of a large pedunculated mass filling the whole uterine cavity, extending from the fundus to the internal cervical os.",yes
PMC4692518,Figure_1,oa_package/a5/59/PMC4692518.tar.gz,[],Supplementary Table1 Correlations of dMRI measures with immunohistological percentage tau burden.,yes
PMC9629544,Figure_2,oa_package/e8/c3/PMC9629544.tar.gz,"['().', 'g002S-2P/NE01 immunization induces B- cell homing to lungs and spleen.']",10.1371/journal.pone.0272594.g002,yes
PMC7716317,Figure_4,oa_package/9a/46/PMC7716317.tar.gz,['\nPositron emission tomography/computed tomography.'],"Figure 4 Positron emission tomography/computed tomography demonstrated an abnormal increased glucose metabolism nodule in the right atrioventricular groove. The maximum standardized uptake value was 21.1, suggesting that the intracardiac mass in the right atrioventricular groove (arrow) was a malignant tumor.",yes
PMC5535563,Figure_6,oa_package/37/ae/PMC5535563.tar.gz,"['On the right, a laminectomy L3 L5 with medial facetectomy/foraminotomy is shown; undercutting preserved the L4 L5 facetsThis parasagittal 3D-CT demonstrated a solid posterolateral noninstrumented fusion 6 months postoperatively.']",Figure 6 This parasagittal 3D-CT demonstrated a solid posterolateral noninstrumented fusion 6 months postoperatively. Note there is continuity of the bone fragments without intervening lucency of the lamina autograft/Nanoss/BMA fusion mass spanning the L4L5 transverse processes in a patient with grade I degenerative spondylolisthesis,yes
PMC11632525,Figure_5,oa_package/3f/96/PMC11632525.tar.gz,[],Figure 4 Jailed diagonal wire with provisional stenting strategy in LAD. D1 remains patent. ( ) Proximal LAD stent before the bifurcation where the stent is well apposed and the D1 is jailed behind the LAD stent. ( ) The bifurcation with diagonal wire comes from D1 ostium. Arrow indicates the jailed wire position. ( ) 3D reconstruction of ostial LAD stent. Both lad and left circumflex wire are luminal.,yes
PMC7428796,Figure_2,oa_package/e5/27/PMC7428796.tar.gz,['Photomicrographs of immunohistochemical staining for phospho-P65 in human samples of pancreatic normal tissues and pancreatic carcinoma tissues.'],Figure 2 Photomicrographs of immunohistochemical staining for phospho-P65 in human samples of pancreatic normal tissues and pancreatic carcinoma tissues. A: Expression of phospho-P65 in pancreatic normal tissues and pancreatic carcinoma tissues; B: (a) Strongly positive expression of phospho-P65 in pancreatic carcinoma tissue (5 ); (b) Weakly positive expression of phospho-P65 in tumor invading edge sample (5 ); and (c) Negative expression of phospho-P65 in pancreatic normal tissue (5 ); C: (a) Strongly positive expression of phospho-P65 in pancreatic carcinoma tissue (20 ); (b) Positive expression of phospho-P65 in pancreatic carcinoma tissue (20 ); (c) Weakly positive expression of phospho-P65 in pancreatic carcinoma tissue (20 ); and (d) Negative expression of phospho-P65 in pancreatic carcinoma tissue (20 ).,yes
PMC11441907,Figure_4,oa_package/8a/e9/PMC11441907.tar.gz,['.'],"Figure 4. MRI examination on November 17 showed that the surface of the liver was rough with multifocal masses of T1 and T2 signal intensities. The right lobe of the liver exhibited enhancement (A), while the venous phase showed uneven enhancement, which was higher than that in the liver parenchyma (B). The arterial phase exhibited a tubular enhancement shadow proximal to the artery (C). MRI = magnetic resonance imaging.",yes
PMC4408054,Figure_2,oa_package/9c/1b/PMC4408054.tar.gz,"['In control cultures without NMDA (A 2D), drebrin A/E was colocalized with f-actin in NeuN-positive cells, as reported previously [E 2H).', 'g002Losses of f-actin and drebrin occur concomitantly after NMDA treatment.', 'In addition to the loss of drebrin, exposure of NeuN-positive mature neurons to NMDA also caused a reduction in the amount of f-actin ().']",10.1371/journal.pone.0125119.g002,yes
PMC3481930,Figure_4,oa_package/e2/99/PMC3481930.tar.gz,"[""Lupus vulgaris with a large epithelioid cell granuloma in the upper dermis with many Langhan's type giant cells (H and E, 100)High power showing Venetian blinds artifact involving the giant cells (H and E, 400)Case 3 is actinomycetoma, with suppurative nodules in the dermis consisting of collections of neutrophils surrounded by histiocytes.""]","Figure 4 Lupus vulgaris with a large epithelioid cell granuloma in the upper dermis with many Langhan's type giant cells (H and E, 100)",yes
PMC4754353,Figure_11,oa_package/39/a7/PMC4754353.tar.gz,[],"Figure 1 Dispersed CMT2D mutations consistently cause neomorphic structural opening at the dimer interface of GlyRS , Distribution of 15 CMT2D-associated dominant mutations in the three domains of the cytosolic human GlyRS. The 3 strongest pathogenic mutations are highlighted in green. Two mutations identified in mice (*) are labeled with their corresponding residue numbers in the human protein. , Human GlyRS structure (monomeric subunit) viewed from dimer interface. Consensus opened-up areas caused by 5 CMT2D mutations are labeled in red . , Opened-up areas (red) by the P234KY mutation (>10 % increase in deuterium incorporation relative to WT GlyRS.)",yes
PMC7441522,Figure_1,oa_package/54/e9/PMC7441522.tar.gz,"['We found that T 1 partially prevented weight loss (A) and increased survival of DSS-treated mice (B).', 'This was associated with an increased colon length (C and D), reduced inflammatory pathology (E and F), decreased production of myeloperoxidase (MPO), TNF- , IL-1 , IL-17A, and IL-17F (G) and increased levels of IL-10 (H).', 'Of interest, T 1 also promoted IDO1 mRNA expression in the colon (H).', '.', 'Because DSS-induced colitis is primarily mediated by innate immune mechanisms with a limited contribution of adaptive immunity (Kiesler et al, 2015) and the anti CTLA-4 treatment is expected to induce a vigorous T cell response, we asked whether the protective effect of T 1 could be still present in the DSS plus anti CTLA-4 model, in which a concerted action of innate and adaptive immunity contributes to gastrointestinal toxicity.']","Figure 1. Thymosin 1 (T1) protects mice from DSS-induced colitis. C57BL/6 mice were subjected to DSS-induced colitis for 1 wk followed by a recovery period of another week. Fresh DSS solution was added at day +3. T1 was administered every other day at the dose of 200 g/kg, intraperitoneally. Mice were evaluated for (A) % weight change, (B) % survival, (C) gross pathology, (D) colon length (cm), (E) colon histology (hematoxylin and eosin staining), (F) histology score, (G) levels of inflammatory cytokines, and (H) IL-10 in colon homogenates and expression. Cytokines were determined by ELISA and gene expression was performed by RT-PCR (data are presented as mean SD of three independent experiments). Images were taken with a high-resolution microscope (Olympus BX51), 20 magnification (scale bars, 200 m). For histology, data are representative of three independent experiments. Each in vivo experiment includes four mice per group. Weight change and survival are calculated on a total of 12 mice per group. * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001, T1-treated versus untreated (DSS) mice. Two-way ANOVA, Bonferroni or Tukeys post hoc test. None, mice with DSS colitis only. Nave, untreated mice.",yes
PMC7341058,Figure_5,oa_package/9d/ba/PMC7341058.tar.gz,"['APOSEC preconditioning led to a significantly lower number of transmural and nontransmural MRI-derived segments, whereas CDC treatment resulted in a significantly lower number of transmural segments in MRI, compared to the untreated AMI group ( 5).', ' 5Number of Heart Segments in Different Groups with Reduced (50% 75%) or Lack of Viability ( 50%) Assessed by [18F]FDG-PET-MRI and Number of Segments with Transmurality 50% and Nontransmural Scar between 25% and 50% Assessed by Cardiac MRIThe number of transmural and nontransmural segments was lower after apoCDC treatment, with fewer transmural segments after CDC treatment compared to controls.']","Figure4 Segmental Analysis of Transmurality and [ F]FDG Uptake of Infarcted Porcine Hearts, 1 Month after Treatment with apoCDCs, CDCs, or Saline Control (A) Significantly lower transmurality was detected in mid-anteroseptal, inferoseptal, and apical segments in both CDC and apoCDC groups than in the untreated control animals. (B) Significantly higher FDG uptake was found in apical-anterior, apical-septal, and apex regions in apoCDC-treated animals and in the apical-anterior segment in CDC-treated animals compared to untreated controls. Data are mean SEM. p< 0.05 (ANOVA with Bonferroni post hoc) compared to AMI untreated.",yes
PMC6537930,Figure_5,oa_package/74/ea/PMC6537930.tar.gz,"['At 2 hrs postinjection, U87MG tumors and surrounding vessels were clearly resolved (A).', 'Tumor imaging using L1013 NPs as NIR-II fluorophore.', '3, surpassing the Rose criterion of 5, which is the threshold at which it is considered possible to allow exact identification of a tumor (B).', '4,5,12With the aid of sharply demarcated tumor imaging at 72 hrs postinjection of L1013 NPs, we were able to precisely resect subcutaneous U87MG tumors (C).']",Figure 5 Tumor imaging using L1013 NPs as NIR-II fluorophore. ( ) Tumor imaging in U87MG tumor-bearing nude mice using L1013 NPs over 72 hrs. All fluorescence images were obtained using a 1250 nm LP filter for signal collection (excitation at 808 nm with a power density of 4.6 mW cm ). Arrows indicate the subcutaneous U87MG tumor. ( ) Tumor-to-normal tissue (T/NT) imaging ratios in L1013 NPs-based tumor imaging over 72 hrs. Data are plotted as mean SD; n=3. ( ) Fluorescence image of an example U87MG tumor-bearing mouse following L1013 NPs (NIR-II) imaging-guided tumorectomy. Inset shows the resected tumor.,yes
PMC10416515,Figure_5,oa_package/f9/be/PMC10416515.tar.gz,"[' 5A).', ' 5B).', ' 5B), which again supports the fact that the L168S ELOVL4 that is targeted to the ER retains some enzymatic activity in contrast to the 5-bp STGD3 mutant ELOVL4 that lacks VLC-PUFA biosynthesis due to loss of its ER localization signal [31].', ' 5C).', ' 5D).', 'L168S ELOVL4 variant is deficient in VLC-PUFA synthesis.', '0001; ns not significant in comparison with WTDiscussion and conclusionsHere, we report the first case of a childhood-onset SCA34 with retinal dysfunction resulting from a novel ELOVL4 variant (NM_022726.']","Fig. 5 L168S ELOVL4 variant is deficient in VLC-PUFA synthesis. Uptake of 20:5n3 after 72h in HEK293T cells overexpressing WT, L168s and control cells (GFP and UT) supplemented with 20:5n3. Relative mole% of VLC-PUFA levels normalized to WT ELOVL4 and L168S ELOVL4 protein levels after 20:5n3 supplementation. L168S mutation decreased the synthesis of the major VLC-PUFA 34:5n3 followed by 36:5n3 found in human retina. Uptake of 34:5n3 after 72h in HEK293T cells overexpressing WT, L168S and control cells (GFP and UT) supplemented with 34:5n3. Elongated products of 34:5n3 normalized to WT ELOVL4 and L168S ELOVL4 expression levels. L168S ELOVL4 protein was deficient in catalyzing the addition of two carbons to produce 36:5n3 similar to cells treated with 20:5n3. Results are the meanSD (n=3). Statistical significance was assessed for , , , by ANOVA with Tukeys post-hoc test. * <0.05; ** <0.01; *** <0.001; **** <0.0001; ns not significant in comparison with WT",yes
PMC7342106,Figure_2,oa_package/4a/8c/PMC7342106.tar.gz,"['586 2A 60 PTCL-NOS T-bet OS 482 d 819 d P=0.', '309 2B 18 NKT T-bet OS 333 d 93 d P=0.', '004 2C 2T-bet T PTCL A 109 PTCL B 60 PTCL C 18 NK-T OS P 0.']",2 T-betTPTCL A109PTCLB60PTCLC18NK-T,yes
PMC11416214,Figure_1,oa_package/79/cc/PMC11416214.tar.gz,"['There was no aortic thrombus at that time, but there was right inferior renal artery occlusion and chronic appearing occlusion of the left iliac artery (Fig 1).', 'Fig 1(Left) Diffusely edematous pancreas with hypoenhancement of the pancreatic head and body.']",Fig1 Diffusely edematous pancreas with hypoenhancement of the pancreatic head and body. Occlusion of the left common iliac artery.,yes
PMC4468200,Figure_1,oa_package/62/b6/PMC4468200.tar.gz,"['cruzi, evidenced by an increase in blood and tissue parasite burden ().', '5-fold decline in blood and skeletal muscle levels of Tc18SrDNA levels, respectively, in chronically-infected/vaccinated WT mice when compared to that noted in chagasic/non-vaccinated WT mice (A 1B, all #p 0.', '6-8-fold lower level of blood and skeletal tissue parasite burden, respectively, than was noted in infected/WT mice before as well as after therapeutic vaccine delivery (A 1B, **p 0.', '2-fold) was observed in response to therapeutic vaccine in chronically-infected WT and GPxtg mice (C, ##p 0.', 'g001Control of T.', 'Mice (GPxtg and WT) were infected, vaccinated and harvested during chronic disease phase as in .', 'Mice (GPxtg and WT) were infected, vaccinated, and harvested during chronic disease phase as in .', 'cruzi and immunized with the two-component D/P vaccine as in .', 'Mice (GPxtg and WT) were infected and vaccinated as in .', 'Importantly, therapeutic delivery of TcG2/TcG4-encoding DNA-prime/protein-boost vaccine arrested the progression to chronic disease phase, evidenced by up to 10-fold control of peripheral and tissue levels of parasite burden as determined by a highly sensitive qPCR approach (), and a significant decline in tissue infiltration of inflammatory infiltrate (Figs shows a correct orientation with distinct presentation of the radiocarpal joint space in both views.', 'Correct aspect of the radio-carpal and radio-ulnar joint in antero-posterior and lateral views.']",Fig. 3 Correct aspect of the radio-carpal and radio-ulnar joint in antero-posterior and lateral views.,yes
PMC3730937,Figure_1,oa_package/d9/53/PMC3730937.tar.gz,"['6 Primary syphilis presents within 3 months of exposure (on average within 2 3 weeks), with a painless solitary ulcer (chancre, see figure 1) and regional lymphadenopathy that may affect the genitalia, mouth or rectum.']","Figure1 Solitary syphilitic chancre affecting (a) male and (b) female external genitalia. A chancre is the archetypal feature of primary syphilis. These ulcers are classically anogenital, solitary, indurated and painless; however, in atypical cases they may be multiple and painful, and can occur at extra-anogenital sites.",yes
PMC5421196,Figure_1,oa_package/04/d3/PMC5421196.tar.gz,['Schematic diagram of T2-weighted axial (a) and coronal (b) images revealing a nonenhancing hyperintense lesion in the right mesial temporal lobe.'],Figure 1 Schematic diagram of T2-weighted axial (a) and coronal (b) images revealing a nonenhancing hyperintense lesion in the right mesial temporal lobe. Axial (c) and coronal FLAIR sequences (d) showing a hyperintense lesion in the white matter of the right mesial temporal lobe,yes
PMC4353684,Figure_5,oa_package/29/66/PMC4353684.tar.gz,['OR memory deficit returns 5 weeks after ChABC treatment in Tg P301S mice PNNs were visualized by WFA staining in P-nase (A) or ChABC (B) injected mice 5 weeks post-injection.'],"Fig. 5 OR memory deficit returns 5weeks after ChABC treatment in Tg P301S mice PNNs were visualized by WFA staining in P-nase (A) or ChABC (B) injected mice 5weeks post-injection. The PNNs present in the PRh of P-nase (a) or ChABC (b) injected mice were shown at higher magnification. C. Stereological quantification of WFA-positive cells in the PRh of P301S mice following P-nase ( =5) or ChABC ( =5) injection. There was no significant difference between groups ( -test, n.s: not significant, =0.072). D. ChABC and P-nase treated P301S mice showed similar level of deficit 5weeks following ChABC injection. At this time point, ChABC treatment was no longer effective in restoring the OR memory in Tg P301S mice (P-nase =7, ChABC =6, -test, n.s, =0.91). E. The exploration time for sample phase of control and P301S mice at 5weeks after ChABC injection, indicating that each group presented comparable extent of motivation and that lack of motivation was not interfering with the results. Control; P-nase =6, ChABC =5, P301S; P-nase =7, ChABC =6.",yes
PMC4554126,Figure_2,oa_package/c4/3f/PMC4554126.tar.gz,[],FIGURE 2 Hematoxylin and eosin stained sections of tumor tissue showing the classic histologic appearance of a small blue cell neoplasm with nests of small round tumor cells with scanty cytoplasm.,yes
PMC9648657,Figure_8,oa_package/e4/3e/PMC9648657.tar.gz,['He underwent frontal remodeling with box craniotomy (extreme left)Illustrative case 6.'],"Figure 8 Illustrative case 6. A 5-year-old child with Apert syndrome who had an FOAR at 14 months now presented with signs of raised ICP. He would require an MD later. Presently, he has no airway issues",yes
PMC8790164,Figure_1,oa_package/f4/9a/PMC8790164.tar.gz,"['3%) (Table 2, ).', '5%)Dermoscopy findings of the patch tests: (a), Homogeneous erythema; (b), Perifollicular erythema (arrow); (c), Papules (arrow); (d), Crusts; (e), Vesicles; (f), Follicular accentuation (atopic patient); (g), Linear vessels; (h), Petechiae (arrow); (i), Pustules; (j), Pore reaction pattern.', 'The morphological vascular alterations comprised punctiform, linear, polymorphic, petechial, and uncharacterized vessels.', 'The images of the patch test reactions, according to the ICDRG, observed with the naked eye and with dermoscopy, are shown in , .']","Figure 1 Dermoscopy findings of the patch tests: (a), Homogeneous erythema; (b), Perifollicular erythema (arrow); (c), Papules (arrow); (d), Crusts; (e), Vesicles; (f), Follicular accentuation (atopic patient); (g), Linear vessels; (h), Petechiae (arrow); (i), Pustules; (j), Pore reaction pattern.",yes
PMC11059464,Figure_5,oa_package/4a/eb/PMC11059464.tar.gz,"['Thick collagen fibers enveloped the entire structure and separated this layer from both the upper SMAS and the underlying bone structures ().', '.']","Figure 5. Serial light microscopy images of hematoxylin and eosin stained sections ( ) and Alcian blue and fast red stained sections ( ) of the zygomatic area. Note the stratified structure of this area and the important structural differences between the superficial hypodermal tissue and the deep hypodermal tissue beneath the SMAS. In the deep hypodermic tissue, the adipocytes appear larger in size. Thick fibers of connective tissue encapsulate this layer between the SMAS and the underlying muscle.",yes
PMC7401041,Figure_5,oa_package/a5/31/PMC7401041.tar.gz,"['This process is displayed in .', '.', '1177_2058738420933099-fig5""/>In our study, results of FCM revealed that the content of Foxp3+ Treg cells was significantly increased among patients with RHL, and ELISA demonstrated that the levels of TGF- were also significantly increased.']","Figure 5. Divergent differentiation of Th17 and adaptive regulatory T-cell (Treg) lineages, emphasizing distinct pathways leading to Th17 effector cells, Foxp3 adaptive Tregs, or Foxp3 adaptive Treg.",yes
PMC11605749,Figure_1,oa_package/cb/fd/PMC11605749.tar.gz,"['Case reportA 76-year-old Caucasian man presented to the dermatology outpatient clinic in early March 2023 with a 2-month history of widespread, sharply demarcated, erythematous, scaly plaques ().', '.', '1177_2050313X241284003-fig1"" position=""float""/>His medical history was significant for a remote history of mild psoriasis affecting his hands and feet (in remission for many years), hypertension, nonischemic cardiomyopathy, heart failure, severe chronic obstructive pulmonary disease (former smoker), and hearing impairment.']","Figure 1. A 76-year-old male with probable drug-induced psoriasis to dapagliflozin, exhibiting well-defined psoriasiform plaques on the trunk, upper extremities, and scalp.",yes
PMC4263764,Figure_5,oa_package/c5/c9/PMC4263764.tar.gz,"['0019) than untreated controls (A).', '0001) (B).', 'g005Reduced LPS and sCD163 levels in plasma with early natalizumab treatment.']",10.1371/journal.ppat.1004533.g005,yes
PMC6769646,Figure_1,oa_package/29/74/PMC6769646.tar.gz,"['Data on the composition of the liver macrophage population and their phenotypic characteristics are summarized in and Table 1.', '005977123555776Sources of development and immunophenotype of a subpopulation of liver macrophages.']",Figure 1 Sources of development and immunophenotype of a subpopulation of liver macrophages.,yes
PMC6591698,Figure_2,oa_package/c8/8a/PMC6591698.tar.gz,['Postoperative AP and lateral radiographs of left proximal femur valgus Osteotomy demonstrating sliding hip screw fixation.'],Figure 2 Postoperative AP and lateral radiographs of left proximal femur valgus Osteotomy demonstrating sliding hip screw fixation.,yes
PMC6198155,Figure_4,oa_package/eb/88/PMC6198155.tar.gz,"['Few neutrophils were also seen ().', 'Re-epithelialization completing under the remained necrotic epidermis in subgroup P8 (H E; Bar = 100 m).']",Fig. 4 Re-epithelialization completing under the remained necrotic epidermis in subgroup P (H & E; Bar = 100m).,yes
PMC7650832,Figure_1,oa_package/fd/b9/PMC7650832.tar.gz,"['After sample quantification of these 51 proteins, 28 were common proteins, 16 proteins only appeared in the control group and 7 in deaths by drowning (A).', 'B shows the number of proteins grouped by their biological functions.', 'Apo A1 and -1 antitrypsin resulted in being differentially expressed, and being less abundant in drowning, whereas apolipoprotein-A1 seemed to be more expressed in drowning (C).', '01720728291(A).']","Figure 1 ( ). Venn Diagram of proteins grouped by the cause of death (obtained from Scaffold viewer). ( ). Number of proteins (grouped by their annotated biological function) commonly expressed in both drowning and control groups. Protein sizes and chromosome locations are available at . ( ) and ( ) Quantitative view of normalization of differentially expressed ApoA1 and -1 Antitrypsin, respectively (obtained from Scaffold viewer).",yes
PMC11558974,Figure_7,oa_package/57/50/PMC11558974.tar.gz,"[' 7).', ' 7a) than SF.', ' 7b).', ' 7c).', '', '0005DiscussionThe results of the current study firstly highlighted a different expression of CD105 and CD90 proteins in the moderate SV group (KL 3) compared to the severe one (KL 3), suggesting an acquisition of CD90 expression, a typical marker of a major subset of fibroblast-like synoviocytes, during the worsening of OA disease [48, 49].']","Fig.7 Synovial fluids analysis. Evaluation of pre-miR203a-3p, miR-203a-3p and SPARC expression levels through qRT-PCR analysis on synovial fluids isolated from patients with KL3 during surgery and plasma isolated 24h before it. Data are reported as log of relative miRNAs or mRNA expression (2^ Ct ) for experimental samples, normalized for U6 or -actin (Mean SD, n = 6). ELISA assay for: IL-1 and SPARCL1 on synovial fluids isolated from patients with KL>3 during surgery and plasma isolated 24h before it. Data are reported as amount of protein released (pg/mL for IL-1 and ng/mL for SPARC) (Mean SD, n = 6). One-way ANOVA, Student t test, Tukey multiple comparisons: *, p<0.05; **, p < 0.005; ***, p<0.0005",yes
PMC9516921,Figure_2,oa_package/95/8f/PMC9516921.tar.gz,['\nComputed tomography scan of intra- and extra-hepatic biliary ducts demonstrated wider dilatation.'],Figure 2 Computed tomography scan of intra- and extra-hepatic biliary ducts demonstrated wider dilatation.,yes
PMC8587732,Figure_2,oa_package/ae/4e/PMC8587732.tar.gz,"['13,20 All mCRPs are expressed by MNs (A), with the highest expression found for CD59, whereas that of CD55 and CD46 was lower.', 'No expression was found for iC3b receptors CD11b (CR3), and CD11c (CR4) (, A and B).', 'Expression of Complement-Regulatory Proteins and Complement Receptors by MN(A) Expression of mCRPs CD46, CD55, CD59, and C3aR, C5aR, CD35, CD11b, and CD11c.']","Figure 2 Expression of Complement-Regulatory Proteins and Complement Receptors by MN (A) Expression of mCRPs CD46, CD55, CD59, and C3aR, C5aR, CD35, CD11b, and CD11c. GM1 staining is shown in green, the indicated membrane protein in red, and DAPI in blue. Images were obtained using a Zeiss Z1 microscope (Carl Zeiss Microscopy) with Colibri LEDs with the following settings: 20 magnification, 25% LED, 400 ms for Alexa FluorTM 488 channel, 100 ms for APC channel, and 50 ms for DAPI channel. Quantification of microscopic data, including isotype controls, is shown in B (mean SD of at least 4 representative individual pictures). DAPI = 4'6-diamidino-2-phenylindole; MN = motor neuron.",yes
PMC3776555,Figure_2,oa_package/b4/b0/PMC3776555.tar.gz,"['The amount of GFP-SLO associated with flat regions of the PM gradually decreased over time, consistent with a toxin internalization process (A,B).', 'Importantly, during the first 60 s after injury GFP-SLO was increasingly detected on 80 nm vesicles containing Cav1, which are properties of caveolae (A,C).', 'The number of 80 nm vesicles positive for Cav1 alone or SLO alone also decreased over time, simultaneously with an increase in the number of 80 nm vesicles containing either Cav1 alone or both Cav1 and SLO (C,D).', '10.', '005.', 'When cells were incubated with the labeled toxin at 4 C, addition of anti-Alexa Fluor 488 antibodies quenched 90% of the fluorescence (E).', 'When the same amount of labeled toxin was added to cells at 37 C, cells were fully permeabilized and about 30% resealed in the presence of Ca2+ under the assay conditions, as indicated by propidium iodide (PI) exclusion (F).', 'By gating on the PI-negative (resealed) cell population we found that at least 50% of the cell-associated Alexa 488-SLO was protected from quenching (G), indicating that it entered compartments no longer in contact with the extracellular medium.', 'See also and Videos 1, 2, 3.', '011Collectively, our assays showing co-localization of endogenous Cav1 and GFP-SLO on 80 nm vesicles (A D), detection of internalized Alexa 488-SLO after extracellular quenching (E G and 3), live imaging of GFP-SLO entering cells in vesicles containing mRFP-Cav1 (B,C and Videos 1 3) and loss of Cav1 and EHD2 co-localization after exposure to SLO (A C) support the conclusion that the pore-forming toxin SLO is removed from the PM in Cav1-positive, caveolae-derived endocytic vesicles.', 'The new data in E G shows that 90% of the labeled toxin was quenched at 4 C, demonstrating that in the absence of endocytosis any SLO bound to cells, including potentially to the lumen of caveolae (given the role of cholesterol as the SLO receptor) remain fully accessible to quenching antibodies.']",10.7554/eLife.00926.004,yes
PMC5521031,Figure_3,oa_package/32/fc/PMC5521031.tar.gz,"['2High-resolution single point dataAnatomical images, R1obs maps, and PSR maps are displayed in for a healthy control and a patient with MS, where the constraints derived from the patient population were used to generate the PSR values.', 'Anatomical data (a.', ')The Bland-Altman plots for the inter-rater comparison are displayed in ', 'The high resolution PSR data in Table 5 and demonstrated significant differences between healthy WM PSR and NAWM, NAGM, and WM-Ls PSR in patients.']","Fig. 3 Anatomical data (a.), R (b.), and PSR (c.) data for a typical healthy control and patient with MS (using the patient-derived parameters). Notice the decreased PSR over areas where a lesion is present, and in the areas surrounding these lesions (lesions outlined in red). (For interpretation of the references to color in this figure, the reader is referred to the web version of this article.)",yes
PMC5666312,Figure_7,oa_package/f9/ad/PMC5666312.tar.gz,[],Figure 4 1 are superior to other spleen antigenpresenting cells for presentation of A and non A antigens,yes
PMC10375370,Figure_1,oa_package/86/1c/PMC10375370.tar.gz,"['Sagittal and axial T2-weighted MRI of the cervical spine reveals extradural dark signal within the posterior spinal canal from C2-C4.', 'To better evaluate the abnormality within the posterior spinal canal, a CT of the cervical spine was performed which revealed fluffy posterior epidural ossification within the spinal canal from C2-C4 that corresponded to the MRI abnormality (']",Fig. 1 Sagittal and axial T2-weighted MRI of the cervical spine reveals extradural dark signal within the posterior spinal canal from C2-C4.,yes
PMC7577845,Figure_11,oa_package/1c/ce/PMC7577845.tar.gz,[],"Fig. 11 Alveolar and vascular pathology in lung. Large-vessel thrombus (arrows) in lung with DAD picture with hyaline membranes (arrowheads). DAD with hyaline membranes (arrowheads) and thrombosis seen in medium- and small-sized vessels (arrows) highlighted with CD61 immunoreactive platelets ( ). Mononuclear mixed perivascular inflammatory infiltrate composed of CD163-immunopositive macrophages ( ) and CD3-immunopositive T lymphocytes ( ). ( , , and hematoxylin and eosin and CD61, CD163, CD3 immunostains; original magnification 40, 100)",yes
PMC10432495,Figure_1,oa_package/17/60/PMC10432495.tar.gz,"['"" id=""os13534-fig-0001"">Entirely posterior type of LHBT labrum attachment of left shoulder: (A) diagram; (B) arthroscopic view with the camera through the posterior portal in glenoid humeral joint; (C).']",Fig. 1 Entirely posterior type of LHBT labrum attachment of left shoulder: (A) diagram; (B) arthroscopic view with the camera through the posterior portal in glenoid humeral joint; (C). arthroscopic view with the camera through the anteriorlateral portal in subarcromial space,yes
PMC3776555,Figure_6,oa_package/b4/b0/PMC3776555.tar.gz,"['To examine the requirement for caveolae in PM repair, we transcriptionally silenced Cav1 expression (A) and examined the ability of cells to reseal after SLO injury using a live imaging assay that follows the influx of the lipophilic dye FM1 43 (Idone et al.', 'After treatment with SLO in the absence of Ca2+ (a condition that does not allow PM repair) there was massive FM1 43 influx, reflecting rapid PM permeabilization (B, Video 4).', 'Quantification of FM1 43 influx showed that cells treated with control or Cav1 siRNA were similarly susceptible to SLO permeabilization (C).', 'In contrast, FM1 43 flowed rapidly into cells treated with Cav1 siRNA even in the presence of Ca2+, reflecting defective PM repair (B,C).', '10.', '012.', 'RNAi-mediated silencing of Cav1 expression inhibits PM repair, allowing sustained FM1 43 influx into SLO-permeabilized NRK cells (related to ).', '013All 80 nm vesicles with caveolae-like morphology observed along the cell periphery were quantified by TEM, and the results indicated that SLO or SM exposure increases the number of caveolae-like vesicles in control cells, but not after treatment with Cav1 siRNA (D,E).', 'Quantification by cryo-immuno EM revealed that 20% of the Cav1 detected on sections of NRK cells was initially associated with flat PM regions without caveolae, and this fraction was reduced to 9% after 60 s of exposure to SLO+Ca2+, along with an increase in labeling of intracellular 80 nm vesicles (F).']","Video 1. Internalization and lateral movement of SLO/Cav1 carriers, (related to ). HeLa cells expressing mRFP-Cav1 (red) were pre-incubated for 5 min with 800 ng/ml of GFP-SLO (green) at 4C and transferred in cold DMEM+Ca to a live imaging chamber at 37C, to allow for progressive warming, pore formation, and PM repair. Images were acquired for 5 min at 2 s/frame on a spinning disk confocal microscope. The video shows a vesicular structure positive for Cav1 and SLO that appears to separate rapidly from the PM and move laterally along the PM before disappearing into a different focal plane. Video is displayed at 6.67 frames/s. Dotted line: PM. Arrow indicates Cav1/SLO carrier. Bars: 5 m.",yes
PMC4891995,Figure_2,oa_package/54/f9/PMC4891995.tar.gz,"['\nOPA1 downregulation decreases mitochondrial respiration, induces the nuclear translocation of NRF2, and increases both catalase quantity and activity in cortical neurons ex vivo.']","Figure 2 1 downregulation decreases mitochondrial respiration, induces the nuclear translocation of 2, and increases both catalase quantity and activity in cortical neurons ex vivo. (A) Representative immunoblots and histograms showing protein levels of 1 (inner membrane), citrate synthase (matrix), 60 (matrix), (outer membrane), and 20 (outer membrane) relative to actin in si 1 (gray bars) and siCtrltransfected (white bars) neurons. Only 1 protein quantity is drastically decreased (0.23 0.06 AU) in si 1 neurons when compared to controls (1.08 0.16 AU). Results are expressed as mean SEM ( = 58). Statistical significance was determined by Student's paired test, *** < 0.001. (B) Oxygen consumption rates were measured at 6 days in vitro ( 6) in neurons transfected with control small interfering (siCtrl, black line) or small interfering against 1 (si 1, dotted line). Spontaneous mitochondrial respiration is significantly lower in si 1transfected neurons (0.48 0.07 pmol/min per g) than in controls (0.92 0.11 pmol/min per g). After 0.6 mol/L oligomycin injection, cell respiration is also significantly lower in si 1 neurons (0.20 0.03 pmol/min per g) than in controls (0.40 0.06 pmol/min per g). After 6 mol/L injection, maximal respiration is significantly lower si 1 neurons (0.44 0.09 pmol/min per g) than in controls (0.59 0.16 pmol/min per g). Finally, 50 nmol/L rotenone and 0.18 mol/L antimycin A injections inhibit mitochondrial respiration. Results are expressed as mean SEM ( = 3). Statistical significance was determined by a twoway analysis of variance ( ), ** < 0.01 and *** < 0.001. (C) over ratio is unchanged in si 1 neurons (gray bar) when compared to siCtrltreated (white bar) cells. Results are expressed as mean SEM ( = 3). (D) Aconitase activity is lower in si 1treated neurons (2.2 0.4 mU/mg) (gray bars) when compared to control cells (3.0 0.5 mU/mg) (white bars). Results are expressed as mean SEM ( = 5). Statistical analysis was determined by Student's paired test, * < 0.05. Representative immunoblots and histogram showing that 1 downregulation in neurons has no effect on the aconitase quantity relative to actin. Results are expressed as mean SEM ( = 6). Statistical significance was determined by Student's paired test, * < 0.05. (E) a: Representative micrographs of 2 immunolabeling (green) and Hoechst staining (blue) in si 1 and siCtrl neurons. b: Histogram represents fluorescence intensity (pixels sum/ m ) of nuclear 2 determined by Image J software, which is higher in si 1 neurons (471.4 8.5 pixels sum/ m ) (gray bars) than in controls (412.8 6.2 pixels sum/ m ) (white bars). Results are expressed as mean SEM (800 cells per condition, = 5). Statistical significance was determined by a twoway , *** < 0.001. Scale bar: 5 m. (F) Catalase activity is increased in si 1 neurons (5.56 0.66 mol/min per mg) when compared to controls (3.44 0.69 mol/min per mg). Results are expressed as mean SEM ( = 5). Representative immunoblots and protein quantities of catalase in si 1 (gray bars) and siCtrl (white bars) neurons relative to actin. Catalase quantity is increased in si 1 (1.6 0.33 AU) when compared to controls (0.85 0.14 AU). Results are expressed as mean SEM ( = 7). Statistical significance was determined by Student's paired test, * < 0.05, ** < 0.01.",yes
PMC10445669,Figure_6,oa_package/2d/cc/PMC10445669.tar.gz,"['The results showed that a blunt force mechanism using the hammer was possible ().', '(A) 3D-model of the surface scanned reconstructed skull (right lateral view).']","Figure 6 (A) 3D-model of the surface scanned reconstructed skull (right lateral view). (B) Virtual repositioned hammer on the fractured skull. For better visualization, the opacity of the hammer has been reduced.",yes
PMC10614774,Figure_4,oa_package/b5/ca/PMC10614774.tar.gz,"['The average scores for each of the 4 sections per mouse knee were then compared within the same treatment group in ().', 'Whole joint scores for synovial hyperplasia, cellularity, and fibrosis were very consistent and did not vary widely from section to section (A-C).', '562259v1-f0003"" position=""float""/>:(A-C) Histopathologic scores of synovial hyperplasia, cellularity and fibrosis respectively summed for 4 joint areas in mice 12-week after sham and PMX surgery (n=5/group).']","Figure 4: (A-C) Histopathologic scores of synovial hyperplasia, cellularity and fibrosis respectively summed for 4 joint areas in mice 12-week after sham and PMX surgery (n=5/group). 4 mid-joint sections per mouse were used to evaluate synovial changes, sections were H&E stained. 4 sets of data are shown in x-axis, each set represents one section per mouse for n=5 mice/group. Kruskal-Wallis test with Dunns post-hoc. Mean 95% CI.",yes
PMC7399638,Figure_1,oa_package/2c/26/PMC7399638.tar.gz,"['There was high variability in the size and distribution of plaques in both groups and studied group regions (A).', 'The highest average immunosignal was detected in the frontal cortex of FAD as well as of SAD patients, followed by temporal cortex, parietal cortex, and occipital cortex, with the lowest A signal being detected in the cerebellum (B and Table 2), creating a predominantly frontal aggregation pattern (C).', 'Two out of 10 SAD showed temporal cortex predominance while 2/10 FAD showed parietal cortex predominance (D).']","FIGURE 1 Distribution of A pathology in brain regions of SAD vs. FAD PS1 E280A. Immunohistochemical staining for total A with 6E10 in frontal cortex (FC), temporal cortex (TC), parietal cortex (PC), occipital cortex (OC), and cerebellum in patients with SAD and FAD (scale bar = 200 mm). Insets depict A deposits at higher magnification for each region (scale bar = 70 mm). Quantification of A immunosignal present in frontal cortex, temporal cortex, parietal cortex, occipital cortex, and cerebellum. No statistically significant differences in all evaluated areas were observed between SAD and FAD cases. Radar chart for the 6E10 percentage of immunosignal distribution according to brain regions in SAD (blue) and FAD (red) cases. Both groups present a FC-predominant distribution pattern. Radar charts for individual 6E10 immunosignal distribution pattern for all SAD (blue) and FAD (red) cases studied. FC predominant distribution pattern was identified among both groups, with seven FAD cases and five SAD cases. TC and PC patterns were identified in SAD and FAD cases, respectively, with two cases for each. Some cases presented dissimilar patterns in both groups, with three SAD cases and one FAD case.",yes
PMC8290623,Figure_2,oa_package/20/82/PMC8290623.tar.gz,"[' 2a).', '2b), however, the Procr mRNA levels remain unchanged (', '2c).', 'CRISPR-gRNA library screening for upstream regulators of Procr.', ' Five regulatory gene candidates were identifiedTo identify the key effectors responsible for changes in Procr protein level, we isolated Procr+ cells from the third passage culture and performed genomic DNA extraction.', '2d).']","Fig. 2 CRISPR-gRNA library screening for upstream regulators of Procr. Procr cells were sorted by FACS and expanded by serial passage. In third passage, a population with high Procr expression emerged. Western blot analysis confirming high Procr protein level in FACS isolated Procr cells. RT-qPCR analysis showing similar Procr mRNA transcription level between FACS isolated Procr and Procr cells. The genomic DNA of FACS isolated Procr cells were extracted and gRNAs which were integrated in the genome were sequenced.Five regulatory gene candidates were identified",yes
PMC3988348,Figure_3,oa_package/7c/65/PMC3988348.tar.gz,"['The final histopathological report by the pathologist confirmed the previous diagnosis of DCIS ().', 'Microscopic examination of breast tumor.']","Figure 3 Microscopic examination of breast tumor. Cribriform-tumor clusters, with architectural and nuclear atypia, consistent with ductal carcinoma (A, H&E stain, 40; B, H&E stain, 200).",yes
PMC10530206,Figure_3,oa_package/72/48/PMC10530206.tar.gz,"['0001) (A).', '0637) (A).', '0434) (B).', 'With respect to learning, we observe non-significant but lower learning in the fear conditioning paradigm for 5xFAD and FAD/Tet1(+/ ) mice versus control measured by total fear cue time or freezing ratio (C.', '044; D, ', '.']","Fig. 3. Loss of Tet1 Increases AD-associated Pathology in 5FAD Mice. A) Results from the forced-swim assay (FST). We measured a significant difference in immobility between 5FAD ( = 14) mice relative to WT ( = 18; p < 0.0001) and Tet1 ( = 9; p 0.0001) mice relative to 5xFAD/Tet1 ( = 10; p < 0.0001). A near significant difference between 5xFAD and 5xFAD/Tet1 was measured ( = 0.0637); two-tailed unpaired t-test. B) The TST identified significant performances between the WT (n = 18) and Tet1 (n = 9; = 0.0397) and 5xFAD/Tet1 (n = 9; = 0.0434) mice; two-tailed unpaired t-test. C) The fear cue portion of the fear conditioning assay found no significant difference in fear memory between conditions across the total time ( = 0.54; two-way RM ANOVA) and their performance normalized for baseline freezing behavior, but a non-significant towards reduced cognition was noted between 5xFAD ( = 15; = 0.2178; two-tailed unpaired t-test) and 5xFAD/Tet1 (n = 10; = 0.1055; two-tailed unpaired t-test) mice relative to WT (n = 18). The performance between 5xFAD/Tet1 relative to 5xFAD is not significantly different. D) Anti-A42 staining in mouse brain (2.5x magnification). The image brightness and contrast were adjusted to improve visibility for the image sections displayed in this figure. Using raw, unprocessed image files, the plaque burden was calculated with the STARDIST machine learning algorithm and identified a significant difference in A42 abundance between 5xFAD ( = 3) and 5xFAD/Tet1 (n = 3) mice ( = 0.044; two-tailed nested t-test). Error bars indicate mean SD; **** p < 0.0001, *** p = 0.00010.001, ** p = 0.0010.01, ** p = 0.010.05, ns p 0.05.",yes
PMC7094508,Figure_1,oa_package/a7/8e/PMC7094508.tar.gz,"['The mucosal surface of the urinary bladder had moderate petechiation (', '', 'xml"">Multimedia component 1\nSupplementary ']","Fig.1 Urinary bladder, Holstein calf (case 4). Multifocal widespread mucosal petechiation. Bar, 3cm.",yes
PMC5088362,Figure_5,oa_package/76/54/PMC5088362.tar.gz,"['Disappearance of pleural sliding, better demonstrated by video.']","Figure 5 Disappearance of pleural sliding, better demonstrated by video. Which is here showed as a drop in the continuity of the line, not moving side by side (by courtesy of Giuseppe Molino, MD, MCAU Ospedale Civile di Ragusa, Italy).",yes
PMC5494868,Figure_7,oa_package/98/db/PMC5494868.tar.gz,"['Recently, it has been reported that the direct depiction of retroperitoneal positioning of the duodenal third portion or the duodenojejunal junction in the left upper quadrant of the abdomen is an alternative diagnostic indication of intestinal malrotation () [8].', '.']","Fig. 7. Normal retroperitoneal course of the duodenal third portion. A, B. A sonogram (A) and an illustration (B) shows normal retroperitoneal course of the duodenal third portion. T-colon, transverse colon; SMV, superior mesenteric vein; SMA, superior mesenteric artery; IVC, inferior vena cava.",yes
PMC11335266,Figure_2,oa_package/b3/53/PMC11335266.tar.gz,"[' 2A-D).', '\nOverexpression of DUXAP8 inhibits the proliferation, migration, and invasion of HTR-8/SVneo and JAR cells.', 'negative control\nAdditionally, RNA-FISH and RT-qPCR of cytoplasmic and nuclear RNA also revealed that DUXAP8 was primarily expressed in the nuclei of primary EVT cells ( S2A).']","Fig. 2 Overexpression of DUXAP8 inhibits the proliferation, migration, and invasion of HTR-8/SVneo and JAR cells. The effects of knockdown or overexpression of DUXAP8 on the proliferation of HTR-8/SVneo cells and JAR cells as detected by CCK-8 ( =3) and colony formation assays ( =3). The effects of knockdown or overexpression of DUXAP8 on the migration and invasion ability of HTR-8/SVneo ( ) and JAR cells ( ) as detected by Transwell assay (without or with Matrigel) ( =3, scar bar =100m). Data are presented as the meanSD, statistical significance was evaluated using the -test or Mann-Whitney U for 2 comparisons, one-way ANOVA and Tukey test or two-way ANOVA for multiple comparison vs. negative control",yes
PMC11509173,Figure_6,oa_package/9c/7f/PMC11509173.tar.gz,"['3 cm) was extracted during the surgery and delivered to the pathology laboratory ().', 'The tumorous tissue extracted during the surgery.']",Figure 6 The tumorous tissue extracted during the surgery.,yes
PMC9764708,Figure_7,oa_package/5f/55/PMC9764708.tar.gz,"[' 7A-F).', ' 7B).', 'Construction of risk prediction model for poorly differentiated CRC.', 'confuse matrix of the modelValidation of risk prediction model for poorly differentiated CRC.']","Fig.7 Construction of risk prediction model for poorly differentiated CRC. We used the relevant functions in the rminer Package (version 1.4.5) of R language for modeling analysis and used the fit function for modeling the variable importance calculation. are the LR model ( ), RF model ( ), NN model ( ), SVM model ( ), GBDT model ( ) and CatBoost model ( ). The left panel (a1, b1, c1, d1, e1, f1) show the variable importance histogram of the model, the upper right panel (a2, b2, c2, d2, e2, f2) show the AUC curve of the model, and the lower right panel (a3, b3, c3, d3, e3, f3) show the CV. confuse matrix of the model",yes
PMC9396039,Figure_1,oa_package/4b/b0/PMC9396039.tar.gz,"[', 2020), involving photoreceptor cells, retinal ganglion cells, bipolar cells, amacrine cells, horizontal cells, glial cells, endothelial cells, pericytes, and RPE cells ().']","FIGURE 1 The structure of retina. The retina comprises at least 10 distinct layers, including eleven cell types involved in the progress of DR. The factors related to these cells, described in this review are shown. (ILM, internal limiting membranes; NFL, nerve fiber layer; GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; ELM, external limiting membranes; OS, outer segments; PEL, pigment epithelium layer; iBRB, inner blood-retina barrier; oBRB, outer BRB; VEGF, vascular endothelial growth factor; VEGFR2, VEGF receptor 2; SDF1, stromal cell-derived factor 1; CYP1B1, cytochrome P450 1B1; GSK3, glucogen synthase kinase 3; Nrf2, nuclear factor erythroid 2-related factor; TRIB3, tribbles homolog 3; TRPC, transient receptor potential canonical; GABA, -aminobutyric acid; ROS, reactive oxygen species; Glut1, glucose transporter 1; CTRP3, C1q/TNF-related protein 3).",yes
PMC8057286,Figure_5,oa_package/48/8b/PMC8057286.tar.gz,['A female patient with severe symptoms.'],Figure 5 A female patient with severe symptoms. HRCT shows multiple ground-glass opacities mixed with consolidation in bilateral lower lobes (white arrows). HRCT: High-resolution computed tomography.,yes
PMC8544178,Figure_5,oa_package/dd/8e/PMC8544178.tar.gz,"['We show that the Fenchol treatment did not change protein ubiquitinylation in the SK-N-SH cells (A), suggesting that the reduction in A accumulation with Fenchol treatment was not mediated through ubiquitin-dependent protein degradation.', 'Fenchol treatment increased proteasome activity, without impacting protein ubiquitination.']","Figure 5 Fenchol treatment increased proteasome activity, without impacting protein ubiquitination. Total protein ubiquitination in Western blots with anti-P4D1 antibody was not impacted by Fenchol treatment. Proteasome activity was significantly increased in Fenchol treated SK-N-SH cells and . Proteasome activity was higher in Fenchol treated SK-N-SH cells that were also treated with A. Interestingly, Fenchol treatment also increased proteasome activity in APP/PS1 mice cortex and hippocampus regions. Data are the mean and standard error of means from three independent experiments done in triplicate.",yes
PMC9797556,Figure_3,oa_package/f8/1d/PMC9797556.tar.gz,"[' 3a).', ' 3B,E).', ' 3C,D), and number of vertices (MFS-CTRL = 5.', 'Normalization of EC morphology after resveratrol treatment in the ascending aorta in MFS mice.', 'As part of the RESV effect are mediated by eNOS46, we evaluated the expression of phosphorylated eNOS (p-eNOS) at serine 1177 in the ascending aorta.', ' 3f).', ' 3g i).']","Figure 3 Normalization of EC morphology after resveratrol treatment in the ascending aorta in MFS mice. ( ) Representative IF confocal images showing -catenin (green), and DAPI (blue) of ascending aorta from 36weeks old MFS mice treated for 3weeks with either vehicle control or RESV. Region of interest (ROI) highlights EC junctional linearity. Arrow indicates the direction of blood flow. Scale bar: 25m. ( ) Violin plot showing quantification of EC alignment normalized to the direction of flow. Data distribution was analyzed by KolmogorovSmirnov test, * indicates <0.05. ( ) Bar plots of EC morphology parameters in the ascending aorta from 36weeks old MFS mice treated for 3weeks with either vehicle control or RESV. All data are represented as meanSD with indicated datapoints for vehicle treatment (N=6; M/F 0/6) n=24 images (total 360 ECs) and RESV treatment (N=6; M/F 0/6) n=24 images (total 360 ECs). ( ) Representative IF confocal images showing p-eNOS (Ser1177) (red) and DAPI (blue) of ascending aorta from 36weeks old WT, MFS mice treated for 3weeks with either vehicle control or RESV. Line of quantification indicated as white line. ( ) Line plot of p-eNOS quantification over a length of 4m perpendicular over the EC membrane in WT, MFS-CTRL, and MFS-RESV. ( , ) Bar plots comparing peak intensity and area under the curve (AUC) of p-eNOS. Data represents mean meanSD with indicated datapoints for WT (N=4; M/F 0/4) n=16 images (total 80 ECs), vehicle treatment (N=4; M/F 0/4) n=16 images (total 80 ECs), and RESV treatment ((N=4; M/F 0/4) n=16 images (total 80 ECs). * <0.05, ** <0.01, *** <0.001, **** <0.0001 analyzed by students t-test or one-way ANOVA with Holm-Sidak post-test.",yes
PMC2953511,Figure_5,oa_package/ad/2e/PMC2953511.tar.gz,"['Additionally, Double staining combined with ISH and IHC revealed that immune cells in selected tissues involved with H5N1 virus RNA were macrophages (co-labeled with CD68 fig. 5A), T lymphocytes (co-labeled with CD3 fig.', '5B), progenitor cells (co-labeled with CD34 fig. 5C) and follicular dendritic cells (co-labeled with CD35 fig.', 'g005Double-labeled stain combined ISH for HA gene and IHC for the cell superfic markers of macrophage CD68 protein, T lymphocyte CD3 protein, progenitor cells CD34 protein and follicular dendritic cells CD35 protein.']",10.1371/journal.pone.0013315.g005,yes
PMC3448196,Figure_3,oa_package/0e/82/PMC3448196.tar.gz,['Multilocular cyst: cyst with internal structure that was predominantly radiolucent and divided into more than one cavity with thin septa (s).'],Figure 3 Multilocular cyst: cyst with internal structure that was predominantly radiolucent and divided into more than one cavity with thin septa (s).,yes
PMC6178322,Figure_4,oa_package/c2/72/PMC6178322.tar.gz,"['The strength and duration of the diffusion gradient detected are referred to as b-values with higher values resulting in the production of a higher signal intensity (A).', 'Calculation of aforementioned b-values facilitates the construction of an apparent diffusion coefficient (ADC) map that demonstrates tumors as having low signal intensity secondary to the increased cell density, reduced interstitial fluid and reduced free water when compared to normal prostatic tissue such as that in a normal gland or in BPH (B).', 'Utilizing diffusion weighted-imaging.']","Figure 4 Utilizing diffusion weighted-imaging. (A) long b axial image, hyperintense tumour lesion right posterolateral zone, (B) hypointense lesion on ADC image.",yes
PMC11322363,Figure_1,oa_package/97/ad/PMC11322363.tar.gz,"['NLRP1, NLRP3, NLRC4, and AIM2 are well-studied sensors for inflammasome formation ().']","FIGURE 1 Major categories of inflammasome sensors. The inflammasome can be classical or non-classical inflammasome. The classical inflammasome is composed of three parts: sensing proteins, adaptors, and effectors. The sensing proteins mainly include NLRP1, NLRP3, NLRC4, and AIM2. The adapter ASC consists of two parts, PYD and CARD. The effectors in the classical inflammasome are Pro-Caspase-1.",yes
PMC8353499,Figure_9,oa_package/68/8e/PMC8353499.tar.gz,"['Radiological findings could be thinning of the small bowel wall, thickening of the intestinal folds and abdominal effusion ( 9).', ' 9Excessive weight loss in a 40-year-old woman 6 years after MGB/OAGB surgery.']","Figure9 Excessive weight loss in a 40-year-old woman 6 years after MGB/OAGB surgery. Coronal T2 weighted MR image shows thinning of small bowel wall, greater intestinal folds representation and abdominal effusion.",yes
PMC11488108,Figure_1,oa_package/26/4e/PMC11488108.tar.gz,['(A) Size distribution of NPs by DLS.'],Scheme 1 Schematic illustration of the preparation procedure of (A) IR780-Mn@TA-TPL NPs and (B) near-infrared fluorescence/magnetic resonance bimodal imaging-guided combinational therapy by IR780-Mn@TA-TPL NPs in AD model.,yes
PMC8004282,Figure_2,oa_package/7c/45/PMC8004282.tar.gz,"['org/1999/xlink"" xlink:href=""rjab087f1"" position=""float""/>\n\nEUS image: homogeneous and hyperechoic lesion in submucosa layer.', 'Endoscopic ultrasound (EUS) also showed a homogeneous and hyperechoic lesion limited to the submucosa (), supporting lipoma diagnosis.']",Figure 2 EUS image: homogeneous and hyperechoic lesion in submucosa layer.,yes
PMC7640573,Figure_3,oa_package/20/db/PMC7640573.tar.gz,"['Cardiac magnetic resonance imaging (MRI) confirmed the diagnosis of non-viable inferior and lateral wall infarction with no reflow, and a small sequela of subendocardial myocardial infarction in the septal and basal wall ().', 'MRI of non-viable inferior and lat ral wall infarction.']",Fig. 3 MRI of non-viable inferior and latral wall infarction. LV =left ventricle.,yes
PMC8160561,Figure_2,oa_package/9c/f7/PMC8160561.tar.gz,"['US-guided percutaneous biopsy confirmed the diagnosis of lymphomaCT, along with US, is an important imaging modality with widespread use for imaging of the spleen.', ' 22).', '', 'Percutaneous image-guided biopsy revealed non-necrotizing granulomas associated with budding yeast consistent with Histoplasma capsulatum infectionCryptococcus neoformans is a cause of infection mostly in immunocompromised patients, particularly in HIV-infected cases.', ' 23) [70].', '', 'Percutaneous image-guided biopsy from the spleen confirmed cryptococcal infectionInvasive aspergillosis is an important cause of morbidity and mortality, especially in immunosuppressed patients.', ' 24).', '', 'Tissue culture grew Aspergillus sppInvolvement patterns in splenic infectious diseases with differential diagnosesBasically, there are four fundamental imaging patterns of splenic involvement in the course of infectious diseases: (1) Splenomegaly without focal lesion, (2) multinodular pattern (representing micro-abscesses), (3) predominantly cystic lesion (larger abscesses), and rarely (4) mass-forming solid parenchymal lesions [1].', ' 25).', '', 'Note was also made of multiple enlarged lymph nodes in the paracaval and paraaortic regions (arrows)The imaging and clinical features of abdominal sarcoidosis are very similar to patients with lymphoma.', ' 2).', ' 26) [81, 82].', ' 27) [83, 84].', '', 'Axial plane postcontrast CT showed large peripancreatic lymphangioma extending into the gastrohepatic and gastrosplenic ligaments (asterisk) and multiple hypodense subcentimeter nodules representing lymphangiomas scattered throughout the splenic parenchyma (arrows)', 'Splenectomy was performed, and histopathological examination confirmed splenic hemangiomatosisSplenic metastases may also be seen as parenchymal nodules or bulky masses in the spleen (', ' 28).', '', 'First-look surgery, with associated splenectomy, confirmed papillary serous tumor of peritoneal surfacesSystemic inflammatory diseases such as hemophagocytic lymphohistiocytosis (', ' 29) may also cause parenchymal nodules and may closely mimic splenic infections or lymphoma both clinically and radiologically.', '', 'Extensive diagnostic workup diagnosed Still s diseaseDespite the fact that they generally do not pose diagnostic difficulty, Gamna-Gandy bodies should be considered in the setting of multiple splenic nodules.']",Fig.2 Diffuse large B-cell lymphoma: 54-year-old female presented with unintentional weight loss and night sweats. Physical examination revealed massive splenomegaly and enlarged axillary lymph nodes. Gray-scale US image demonstrated multiple subcentimeter hypoechoic nodules scattered throughout the splenic parenchyma (arrowheads). Axial plane postcontrast abdominal CT performed the next day after the initial US examination showed only massive splenomegaly (asterisk) with no discernible parenchymal nodules. US-guided percutaneous biopsy confirmed the diagnosis of lymphoma,yes
PMC7578749,Figure_8,oa_package/6a/2f/PMC7578749.tar.gz,[],"Figure7 p32cKO Mice are Susceptible to Hemolysis Due to Erythroid Differentiation Failure (A) KaplanMeier plot of age-matched WT and p32cKO mice (n= 8 per group) treated with PHZ (80mg/kg). (B) Hb levels in peripheral blood from WT (n= 8) and p32cKO (n= 8) mice treated with PHZ (80mg/kg). (C and D) Representative flow cytometry plots (C), and proportions and absolute numbers (D) of Ly6D CD44 CD51 TNCs in enzymatically digested bone marrow from WT and p32cKO mice after PHZ injection. n= 4 mice per group. (E) Quantification of Ter119 (erythroid) and B220 (B-lymphoid) cells that were differentiated from sorted Ly6D CD44 CD51 TNCs of WT and p32cKO mice seeded in liquid culture with cytokines for 48hr n= 3 mice per group. (FH) Analysis of Torin 1-treated mice after PHZ injection. n= 8 mice per group. Hb levels in the peripheral blood (F) and KaplanMeier plot of WT mice (n= 8) treated with PHZ (80mg/kg) after Torin 1 (20mg/kg) injection (G). Quantification of Ter119 (erythroid) and B220 (B-lymphoid) cells that were differentiated from sorted Ly6D CD44 CD51 TNCs of WT mice (H). Cell numbers in each population were normalized as the percentage of total cells plated per well (% of cells plated). Data are shown as means SD. p< 0.05 versus WT mice or DMSO controls. Data are representative of three independent experiments. See also .",yes
PMC10406435,Figure_15,oa_package/9a/8c/PMC10406435.tar.gz,[],"Figure 9. CAF-382 ( ) did not alter NMDAR-mediated component of fEPSPs. Maximal fEPSPs recorded in stratum pyramidale of CA1 with reduced extracellular Mg demonstrate multiple population spikes; previous studies have demonstrated that secondary population spikes in these conditions are NMDAR-dependent ( ). CAF-382 ( ) (100 nM) did not alter population spike 1 (control 4.057 0.584 mV, CAF-382 ( ) 3.929 0.736 mV, n = 10, p=0.742, RM-ANOVA) or NMDAR-mediated population spike 2 (control 2.093 0.568 mV, CAF-382 ( ) 1.656 0.4442, n = 10, p=0.867, RM-ANOVA). For comparison, the selective NMDAR antagonist D-APV (50 M) completely blocked population spike 2, as previously reported ( ). Stimulation artifact has been removed (arrow) for clarity. fEPSP, field excitatory postsynaptic potentials.",yes
PMC5563341,Figure_1,oa_package/f5/79/PMC5563341.tar.gz,"[' 1a).', '', 'Wilcoxon matched pairs test was used to calculate significance\nEffector memory CD8+ T cells are the main T-cell subset in NAWM and WML of MS patientsTo determine the phenotype and differentiation status of T cells in MS patients ex vivo, paired PB, CSF, and lymphocyte-enriched NAWM- and WML-derived single cell suspensions of 17 MS patients were subjected to multiplex flow cytometric analysis.', ' 1c).', ' 1c) [10].', ' 1d).']","Fig.1 CD8 T cells in normal-appearing and diseased white matter tissues of MS patients preferentially express an effector memory phenotype. , Parenchymal T cells are selectively detected in active MS lesions. 10-m cryostat sections of paired ( ) normal-appearing white matter (NAWM) and ( ) white matter lesion (WML) of two representative patients of five MS patients analyzed. CD3 expressing cells (T cells) were stained with 3-amino-9-ethylcarbazole ( ) and counterstained with hematoxylin ( ). Whereas perivascular T cells were detected in both NAWM and WML, parenchymal T cells were exclusively detected in active WML of MS patients. Percentages of CD4 and CD8 T cells, and CD4 /CD8 T-cell ratio, are shown for paired PB ( ), CSF ( ) and histologically defined as NAWM ( ) and WML ( ). Lymphocytes were isolated from paired peripheral blood (PB), cerebrospinal fluid (CSF), NAWM and WML (lesion) from patients with advanced MS ( =17) and subjected to multiplex flow cytometry. Gating procedure of CD4 and CD8 T cells is shown in Online Resource 3. CD8 T cells were subdivided in nave (CD27 CD45RA ), central memory (CM; CD27 CD45RA ), effector memory (EM; CD27 CD45RA ) and terminally differentiated effector memory (EMRA; CD27 CD45RA ) T cells. Gating procedure is shown for representative paired PB and WML-derived CD8 T cells. The frequency of nave, CM, EM and EMRA CD8 T cells is shown for paired PB, CSF and white matter brain tissues that were immunohistologically classified as NAWM, diffuse white matter abnormalities (DWMA), active lesions (AL), mixed active/inactive lesions (mIAL), inactive lesion (IL) or unconfirmed white matter tissues (UWM) (see Online Resource 3 for criteria applied for MS WM classification). represent the mean frequencies. Wilcoxon matched pairs test was used to calculate significance",yes
PMC8325528,Figure_3,oa_package/67/63/PMC8325528.tar.gz,"['Images of two ductal carcinoma in situ (DCIS) cases.', '3a) involved a 55-year-old white female with a 5.', '3b) involved a 63-year-old black female with a 3.']",Fig. 3 Images of two invasive lobular carcinoma cases. Arrows indicate positive margins identified in volumetric specimen imager (VSI) images,yes
PMC8695471,Figure_2,oa_package/37/50/PMC8695471.tar.gz,"['The FNM cases showed a circular, notch-like corticated defect on the inferior portion of the basiocciput ().', 'Mid-sagittal cone-beam computed tomographic image shows notch-like defect within the pharyngeal portion of the clivus.']",Fig. 2 Mid-sagittal cone-beam computed tomographic image shows notch-like defect within the pharyngeal portion of the clivus. This is one of the classic appearances of fossa navicularis magna.,yes
PMC3511355,Figure_5,oa_package/b8/96/PMC3511355.tar.gz,"['As shown in , decreased fungal burdens (A) and Foxp3+ Treg cells (CD4+CD25+Foxp3+), which were associated with increased numbers of effector CD4 T (CD44highCD62low) cells, were detected at week 2 post-infection (', 'Although less extreme than that observed with the anti-CD25 antibodies, a decreased number of FoxP3+ cells was observed (F H).', 'g005Anti-CD25 treatment depletes Foxp3GFP Treg cells and induces decreased fungal loads in Foxp3GFP C57BL/6 mice.']",10.1371/journal.pone.0051071.g005,yes
PMC9605814,Figure_2,oa_package/1e/74/PMC9605814.tar.gz,['CT scan of the head in the immediate perioperative period after dedicated catheter angiogram study.'],Figure 2 CT scan of the head in the immediate perioperative period after dedicated catheter angiogram study.,yes
PMC5796651,Figure_11,oa_package/a7/91/PMC5796651.tar.gz,[],"Figure 1 Astrocyte morphogenesis occurs in tune with sensory activity , V1 cortex images (layers L1-L6) from Aldh1L1-EGFP mice at postnatal days P1-P21. , Fold change in astrocyte coverage of the neuropil at each cortical layer from P1-21 (normalized to P1 L1). , Fold change in astrocyte coverage of the neuropil from P7 and P21 (normalized to P7). - , n=10 ROI/layer, >3 images/mouse, 3 mice/time point. , Representative images and neuropil infiltration volumes (NIV) of V1 L4 PALE astrocytes from normal (NR) and dark reared (DR) mice at P7 and P21. Astrocytes were electroporated with EGFP (green) and membrane-tagged mCherry (mCherry-CAAX, red) plasmids. , Average NIV of P7 and P21 astrocytes from NR and DR mice. n = 3 NIV/cell, 18-20 cells/condition, 4 mice/condition. One-tailed -test ( ), one-way ANOVA ( ). Data are means s.e.m. Scale bars, 100 m ( ), 10 m ( ).",yes
PMC9592549,Figure_6,oa_package/05/71/PMC9592549.tar.gz,[],"Figure6 Translational studies in healthy and diseased human livers. Normal liver sample Nr. 5 ( ), NAFLD sample Nr. 9 ( ) and viral hepatitis sample Nr. 7 ( ) tissues were stained with ORO for lipid (red) and HE for nuclei (blue). Human livers - as described in Figure6A - were stained with ApoE (red), C1q (white), and DAPI (blue) showing similar staining patterns vs mouse livers. Dotted lines demarcate immune cell infiltration sites from adjacent liver parenchyma. Human livers as described in Figure6A - were stained with C5 (red), CD68 (green) and DAPI (blue) indicating nuclei. Representative images of human samples ( ) are shown. Dotted lines demarcate immune cell infiltration sites from adjacent liver parenchyma. Scale bars 100 m.",yes
PMC10689373,Figure_1,oa_package/fe/16/PMC10689373.tar.gz,"['On microscopy, a well-demarcated spindle cell tumour was observed beneath a grenz zone (figure 1).', 'Grenz zone between epidermis and dermal spindle cell tumour with rim of inflammatory cells (H E 20).']",Figure 1 Grenz zone between epidermis and dermal spindle cell tumour with rim of inflammatory cells (H&E20).,yes
PMC3670857,Figure_6,oa_package/ef/69/PMC3670857.tar.gz,"[', CXCL2 or MIP-2), and other oligodendrocyte mitogens such as FGF-2 and PDGF-BB () show that there is a significant and progressive elevation in their levels with time in the spinal cord of Twitcher mice.', 'g006Altered cytokine and growth factor levels in the spinal cord of Twitcher mice that could possibly compensate for the lack of KC or CXCR2.']",10.1371/journal.pone.0064647.g006,yes
PMC8785281,Figure_3,oa_package/92/d9/PMC8785281.tar.gz,"['DiscussionFascicular-ventricular pathways originate from the fascicles such as the His bundle or bundle-branch and insert into ventricular myocardium\n1\n ().', '\n2\n.', '1177_23247096211073261-fig3"" position=""float""/>Differentiating Pre-excitation Due to Fascicular-Ventricular Connections From Wolff-Parkinson-WhiteA feature of FVP which is not seen in typical AV accessory pathway is the same degree of pre-excitation with a longer PR interval.']","Figure 3. Fascicular-ventricular connections (red arrow) connects one of the fascicles of the conduction system to the ventricular myocardium thus resulting in delta waves (pre-excitation). As this connection is distal to the AV node the P wave and the PR interval are fully inscribed before the delta wave begins. The AV connections responsible for WPW are from atrium to the ventricular myocardium (blue arrow). Abbreviations: AV, atrioventricular; WPW, Wolff-Parkinson-White.",yes
PMC4949397,Figure_4,oa_package/0d/e2/PMC4949397.tar.gz,['Effects of the Hp knockout on anatomical outcomes following CCI in different age cohorts.'],"Figure 4 . The representative microphotographs of cresyl violet-stained brain sections obtained at 48 h after CCI in adult and older adult WT and Hp mice, respectively. In each panel, three examples of the brain sections from three different animals from the same experimental group (marked as Mouse #13). The example brain section from each mouse was cut within coordinates from 1 to 2 mm. Areas filled with red represent cavitation and red dotted lines represent boundaries of cortical areas covered by cortical lesions used for quantitative histopathological analyses. Comparison of the lesion volumes between CCI-injured WT and Hp mice in the adult and older adult age cohorts , and between the CCI-injured WT mice from different age cohorts , respectively. Comparison of relative hippocampal volumes between WT and Hp CCI-injured mice in adult and older adult age cohorts , and between CCI-injured Hp mice of different ages , respectively. The numbers shown on the graphs represents -values from the multi-factor ANOVA an statistical analyses performed using Student's -test to compare values between matched groups ( = 410).",yes
PMC10659529,Figure_2,oa_package/9e/e7/PMC10659529.tar.gz,"['Furthermore, resting membrane potential and AP threshold were significantly different for PV cells when compared by region (Extended Data , bottom).', '4), an isoform with increased expression in human AD24,25 was expressed using the pan-neuronal EF1a promoter (a; AAV.', 'In LEC PV interneurons, we observed a dramatic reduction in PV interneuron firing (b,c) likely related to related to a reduction in input resistance (', 'The presence of hAPP mRNA and protein was confirmed in PV neurons 2 3 weeks after injection (i j; Extended Data ', 'Extended Data .', '.']","Extended Data Figure 2. Passive and active properties of LEC PV interneurons after hAPP injection Graphical summary of AAV.E2.tdTom and AAV.EF1a.hAPP (or for Ctrl, saline) stereotactic injection in the Lateral Entorhinal Cortex. tdTom+ PV interneurons were fluorescently targeted for whole-cell current-clamp recordings. AP waveforms of tdTom+ PV interneurons were compared at 12 pA/pF square pulse injections in WT mice from Ctrl and hAPP injected. Aps from the 1 spike in the train are superimposed for comparison. Relationship between AP amplitude (p=0.8848, df=21) or width (p<0.0001, df=21) in WT mice and AP # during spike trains elicited with a 12 pA/pF current injection. Summary data of AP properties. LEC PV interneurons after hAPP injection 1 AP amplitude (p=0.9365, t=0.0809) and half-width remains unchanged (p=0.5096, t=0.6746, df=21). Summary data of AP properties. LEC PV interneurons after hAPP injection AP threshold (p=0.8471, t=0.1964), dV/dt max (p=0.1228, t=1.624), and AHP (0.6284, t=0.4929) (df=21) remained unchanged. Summary data of AP properties. LEC PV interneurons after hAPP injection show unchanged Resting Membrane Potential (p=0.0898, t=1.788), Rheobase (p=0.0516, t=2.070) and Accomodation Ratio (p=0.6616, t=0.4443), but a reduction in Membrane Tau(p=0.0008, t=3.933) (df=21). For all summary graphs, data are expressed as mean ( SEM). For : Statistical significance is denoted as *=p<0.05, as determined by Two-way ANOVA with Sidaks multiple comparison test. For c, d, e: Individual data points and box plots are displayed. Statistical significance is denoted as *=p<0.05, as determined by two-tailed unpaired t-test.",yes
PMC10628574,Figure_6,oa_package/35/75/PMC10628574.tar.gz,"['Pathological findings: HE staining, 40 ; under the microscope, thyroid tissues were observed within the pancreatic tissues, approximately 2.', 'Postoperative pathological examination demonstrated follicle-like structures of variable size with mild cell morphology in the pancreas, based on which ectopic thyroid tissues were suspected ().']","Figure 6 Pathological findings: HE staining, 40; under the microscope, thyroid tissues were observed within the pancreatic tissues, approximately 2.5cm 2.0cm 1.6cm in size, with follicles of varying sizes and mild cell morphology.",yes
PMC2789962,Figure_1,oa_package/74/09/PMC2789962.tar.gz,"['On fundoscopy, there was a dumbbell-shaped macular bleed with a well-defined margin in the left eye ().', '24th edSt LouisMosby73777415ChoiSWLeeSJRahSHValsalva retinopathy associated with fiberoptic gastroenteroscopyCan J Ophthalmol20064149149316883367A fundus photo (OU) obtained at the initial examination.']",Fig. 1 A fundus photo (OU) obtained at the initial examination. This photo of the left eye demonstrates a preretinal hemorrhage at the fovea.,yes
PMC8629676,Figure_4,oa_package/75/4b/PMC8629676.tar.gz,"['Given the natural history that this AVM is likely to continue to expand over time and would be amenable to occlusion, the patient underwent successful transcatheter occlusion with 17 large detachable coils ().', '003"" position=""float""/>Images from the conclusion of the case demonstrating the mass of coils after occlusion (a) and filling of only normal arterial structures (b) on the postintervention angiogram.']",Figure 4 Images from the conclusion of the case demonstrating the mass of coils after occlusion (a) and filling of only normal arterial structures (b) on the postintervention angiogram.,yes
PMC3980213,Figure_4,oa_package/02/3b/PMC3980213.tar.gz,"['Aurora A was positive both in Alcian blue-negative cells (a) and in cells with initial production of Alcian blue-positive mucins, but lacking of goblet cell morphology (cells with intermediate features between IM and CLO) (b).', '.']","Figure 4. Group C. Mucosa with a few cells with intermediate features between intestinal metaplasia and columnar-lined oesophagus, showing initial production of Alcian blue positive mucins, but lacking of goblet cell morphology. a) Aurora A positivity both in Alcian blue negative cells (arrows); double Aurora A/Alcian blue stain. b) Aurora A positivity in cells with initial production of acid mucins (arrows); triple Aurora A/Alcian blue/PAS stain).",yes
PMC10690071,Figure_9,oa_package/0c/d5/PMC10690071.tar.gz,[],Figure 9 Post-operative follow-up revealing satisfactory wound healing (arrow),yes
PMC6257653,Figure_1,oa_package/d6/2d/PMC6257653.tar.gz,['Pre-operative anterior/posterior X-rays.'],Figure 1 Pre-operative anterior/posterior X-rays. Pre-operative anterior/posterior X-rays demonstrating multi-level disc degeneration and neuroforaminal stenosis most severe in the lower lumbar spine.,yes
PMC4001432,Figure_1,oa_package/af/66/PMC4001432.tar.gz,"['Case reportPatient 1A 33-year-old man injured in a motorcycle accident had sustained compound open fractures of the left tibia and fibula with remarkable backward dislocation of the bone fragments (a).', 'A left lower extremity angiogram showed complete occlusion from the popliteal artery to the tibioperoneal trunk and the origin of the anterior tibial artery (b).', 'Then, stepwise inflation with three balloon catheters of 3-, 4-, and 5-mm diameters and 4-cm length was performed 10 times for 3 min (c).', 'During the procedure, we observed gradual joining of the intimal flap to arterial wall and sufficient peripheral blood flow through the repaired true lumen (d).', '.', '1177_2047981613518772-fig1""/>Satisfactory peripheral blood flow was maintained with anticoagulation therapy (continuous administration of intravenous heparin, 15,000 U/day).', 'Emergency angiography showed re-occlusion of the popliteal artery caused by formation of a thrombus adhering to the intimal flap (e).', 'After the two sessions of endovascular treatment, adequate blood perfusion was maintained (f), and the patient was moved to a rehabilitation hospital 55 days after admission.']",Fig. 1. A 33-year-old man with popliteal artery occlusion due to blunt trauma (Patient 1). (a) Radiograph of the left knee showing compound fractures of the tibia and fibula with remarkable backward dislocation. (b) Left lower extremity angiogram showing complete occlusion from the popliteal artery to the tibioperoneal trunk and origin of the anterior tibial artery. (c) Stepwise balloon angioplasty with long inflation time. (d) Recanalization by joining of the intimal flap to arterial wall. (e) Left lower extremity angiogram showing re-occlusion of the popliteal artery caused by formation of a thrombus adhering to the intimal flap (arrow). (f) Restoration of sufficient blood flow after aspiration thrombectomy.,yes
PMC9705148,Figure_1,oa_package/dd/47/PMC9705148.tar.gz,"['1\nTumors in the body (\n\n) and tail (\n', '\nPancreatic ductal adenocarcinoma.', 'MPD appears normal (\n1\n).', '1\nIntraductal papillary mucinous neoplasm (\nA\n) Axial and (\nB\ncoronal venous phase image showing three well defined cystic lesions in the pancreatic body and tail (\nwhite arrows\n) that are seen communicating with the main pancreatic duct (MPD) (\nred arrow\nin B).', '30\nAdenocarcinoma Arising in IPMN\nInvasive cancer arising from IPMN has distinctive radiologic appearances (\n2\n); risk of malignancy is significantly higher in MD-IPMNs than BD-IPMN.', ' \n31\n2\nIntraductal papillary mucinous neoplasm with adenocarcinoma.', 'Calcifications and heterogeneously enhancing solid areas may be present at the periphery of the mass, which are iso- to hypoattenuating to the normal pancreatic parenchyma on arterial and venous phase (\n3\n).', '33\n3\nSolid pseudopapillary tumor.', 'They appear as well-circumscribed hypodense lesions on CT that are usually hypoattenuated compared to the normal pancreas on CE-CT in case of primary hypovascular tumors (lung, colonic, gastric cancers) (\n4\n) and hyperattenuated in case of primary hypervascular tumors (HCC, thyroid, renal cell cancers).', '4\nPancreatic metastases.']",Fig. 1 Pancreatic ductal adenocarcinoma. Axial ( ) and precontrast ( ) pancreatic parenchymal phase and ( ) venous phase image showing an ill-defined hypodense hypoattenuating exophytic lesion arising from the body of pancreas ( ).,yes
PMC7555214,Figure_5,oa_package/b5/1d/PMC7555214.tar.gz,"['As shows, both drugs were able to reduce CYP2E1 protein levels to control values (A).', 'The protective effect of CYP2E1 inhibition and ROS blockade resulted in an increase in cell survival, reaching the values of the control group (B).', 'This improvement of cell survival correlates with the significant reduction in intracellular superoxide anions (C) and total intracellular ROS (D).', 'However, the current work reveals that EtOH-induced oxidative stress can upregulate CYP2E1 expression and activity by itself, inducing RPE cells alterations ().', 'EtOH-induced oxidative stress promotes CYP2E1 upregulation and cell death.']",Figure 5 EtOH-induced oxidative stress promotes CYP2E1 upregulation and cell death. ( ) CYP2E1 protein levels in ARPE-19 after 600 mM EtOH treatment and 20 mM DAS or 4 M NAC measured by WB. ( ) Cell viability by XTT assay. ( ) Superoxide anions by DHE fluorescence and ( ) Total intracellular ROS by DCFH fluorescence. Protein levels were normalized by -Actin. Values are expressed as mean SEM (N = 3). Statistically significant differences were set at * < 0.05.,yes
PMC7466895,Figure_2,oa_package/12/33/PMC7466895.tar.gz,"['1177_2325967120946312-table2""/>Systemic illnessYesNoStatic and dynamic alignmentYesNoOnsetAbruptProgressiveTime since onsetAcuteChronicType of painSuperficialDeepNeovascularizationYesNoDegenerative areaYesNoSizeFocalizedDiffusedLocationEnthesis, tendonVentral, dorsalIn , for instance, we can see how the ventral surface of a patellar tendon is affected.', '.', '1177_2325967120946312-fig2""/>ConclusionDiagnosing patellar tendinopathies requires application of a complex and thorough assessment process for each individual case and should include all variables that basic sciences have provided.']",Figure 2. Sagittal magnetic resonance image showing injury on the ventral side of patellar tendons proximal insertion.,yes
PMC7993245,Figure_5,oa_package/e0/5d/PMC7993245.tar.gz,"['fig5a""/>b:Examples of examination-level annotations on axial CT images.', 'fig5b""/>c:Examples of examination-level annotations on axial CT images.', 'fig5c""/>d:Examples of examination-level annotations on axial CT images.', 'fig5d""/>e:Examples of examination-level annotations on axial CT images.', 'fig5e""/>f:Examples of examination-level annotations on axial CT images.', 'fig5f""/>g:Examples of examination-level annotations on axial CT images.', 'fig5g""/>h:Examples of examination-level annotations on axial CT images.', 'fig5h""/>i:Examples of examination-level annotations on axial CT images.', 'fig5i""/>j:Examples of examination-level annotations on axial CT images.', 'fig5j""/>k:Examples of examination-level annotations on axial CT images.', 'fig5k""/>l:Examples of examination-level annotations on axial CT images.', 'fig5l""/>m:Examples of examination-level annotations on axial CT images.', 'fig5m""/>Several decisions among a panel of five senior thoracic radiologists (J.']","Figure 3c: Example of CT scans and chest radiographs in the RSNA International COVID-19 Open Radiology Database. Annotated axial CT image shows segmentation of characteristic bilateral multifocal ground-glass opacities in predominantly peripheral distribution (orange regions of interest). The CT image was classified as having typical appearance of coronavirus disease 2019 (COVID-19) pneumonia. Annotated axial CT image shows segmentation of bilateral multifocal ground-glass opacities with diffuse distribution (orange regions of interest). The CT image was classified as having indeterminate appearance of COVID-19 pneumonia. Thoracic CT image shows bilateral nodular and patchy opacities with peripheral and lower lung predominance involving four lung zones, annotated as typical for COVID-19 with moderate severity. Thoracic CT image shows bilateral nodular and patchy opacities with peripheral and lower lung predominance involving more than four lung zones, annotated as typical appearance for COVID-19 and severe lung involvement. Bedside chest radiograph with bilateral patchy and nodular opacities (arrows) with upper lung predominance involving more than four lung zones, annotated as indeterminate appearance for COVID-19 and severe lung involvement. Bedside chest radiograph shows left lower lobe opacities (arrows) with small left pleural effusion involving a single lung zone, annotated as atypical appearance for COVID-19 and mild lung involvement. Bedside chest radiograph shows bilateral patchy and nodular opacities (arrows) with upper lung predominance involving more than four lung zones, annotated as indeterminate appearance for COVID-19 and severe lung involvement. Bedside chest radiograph shows left lower lobe opacity (arrow) with small left pleural effusion involving a single lung zone, annotated as atypical appearance for COVID-19 and mild lung involvement.",yes
PMC4192061,Figure_3,oa_package/88/ab/PMC4192061.tar.gz,"['As was observed in many LSDs, ML1-null muscle exhibited a compensatory increase in the key lysosomal protein Lamp113, as shown by Lamp1 immunofluorescence staining and western blot analysis (Supplementary a,b).', 'However, when compared with the adjacent noninfected and contralateral uninfected muscle fibers, ML1-null Gastroc muscle infected with AAV-GFP-ML1 (localized in Lamp1-positive compartments; see Supplementary c,d) experienced a dramatic AAV-infection mediated decrease of elevated Lamp1 expression (', '2g Supplementary e).', 'However, no obvious decrease in expression was observed for any of core and accessory components of the DGC being examined, which included dystrophin, -DG, integrin 1, and laminin (a,b).', 'Furthermore, the expression of dysferlin, cav-3, and MG53, three proteins known to be involved in sarcolemma repair and human MD4,5,23, also exhibited no decrease in ML1-null muscle (a,b).', 'Rapid FM dye entry and accumulation were observed within seconds after laser irradiation (c Supplementary ', 'However, in WT muscle fibers, dye entry ceased shortly (1 2 min) after irradiation, suggesting successful membrane resealing (c).', 'In contrast, upon identical laser irritation, ML1-null fibers continued FM dye uptake at the injury sites for several minutes (c), suggesting failed membrane resealing.', 'Defective membrane resealing was also observed in ML1-null myotubes that were exposed to mechanical damage elicited by microelectrode penetration into the sarcolemmal membrane (d).', 'No significant FM4 64 dye was seen at injury sites, even with repeated penetrations (e).', 'In the presence of BAPTA-AM to chelate intracellular Ca2+ or glycyl-L-phenylalanine 2-naphthylamide (GPN), a lysosome-targeted cathepsin C substrate, to specifically deplete the lysosomal Ca2+ store13, significant dye entry was observed even under normal extracellular Ca2+ concentrations (2 mM; e).', 'Notably, in experiments performed by researchers who were blind to experimental conditions, significant FM4 64 dye uptake was seen in the presence of three structurally independent ML-SI compounds (e Supplementary ', 'Notably, co-injection of ML-SI3 with CTX markedly increased the percentage of EB-positive muscles in CTX-treated WT mice, to the same level as that of CTX-treated ML1-null mice (f Supplementary ', 'Defective membrane repair capacity in ML1 KO muscle(a) Immunofluorescence of dystrophin, -dystroglycan ( -DG), integrin 1, laminin, caveolin-3 (Cav-3), and dysferlin in ML1-null Gastroc muscle.']","Figure 3 Defective membrane repair capacity in ML1 KO muscle Immunofluorescence of dystrophin, -dystroglycan (-DG), integrin 1, laminin, caveolin-3 (Cav-3), and dysferlin in ML1-null Gastroc muscle. Scale bar = 10 m. Western blotting analysis of the DGC components, Cav-3, dysferlin, and MG53 in ML1 KO mice. Myosin served as a loading control. A membrane repair assay performed on single FDB muscle fibers isolated from WT and ML1 KO mice. Membrane damage was induced with a two-photon laser at t = 0 s. Scale bar = 10 m. The right panel shows the time-dependent changes (F) in FM143 fluorescence intensity normalized to the basal fluorescence (F ) for WT (blue) and ML1 KO (red) fibers. Representative images of differentiated myotubes in response to mechanical damage elicited by a microelectrode (arrows). The lower panel shows the percentage of surviving myotubes in response to microelectrode penetration. The experiments were performed in the presence of extracellular Ca , and defective membrane resealing led to excessive Ca influx that triggered prolonged muscle contraction. The surviving cells are those without prolonged contraction. Scale bar = 50 m. Responses of C2C12-derived myotubes to microelectrode penetration in Tyrode's solution (2 mM Ca ) in the presence of DMSO (0.1%; 1 and 2 penetration), ML-SI3 (20 M), BAPTA-AM (20 M), and GPN (200 M). The right panels show the time course of FM464 accumulation (F/F ) at injury sites following microelectrode penetration. Scale bar = 50 m. Note that the same set of DMSO control data was compared with all groups of drug treatment. The effect of ML-SI3, a TRPML-specific synthetic inhibitor, on EB dye uptake in WT Gastroc muscles injected with the cardiotoxin VII4 (CTX), a cytolytic toxin that disrupts cell membrane in living animals . The lower panel shows that intramuscular coinjection of ML-SI3 with CTX on EB dye uptake in WT and ML1 KO muscle. Scale bar = 10 m. Data are presented as the mean s.e.m, = 3 animals for each condition.",yes
PMC8906146,Figure_2,oa_package/00/d2/PMC8906146.tar.gz,"['Multiple factors may have contributed to this not being\nidentified, including the more prominent findings in the right pelvis (infected\nhaematoma), the distance from the surgical site, the relative lack of adjacent fat\nstranding and the presence of a small surrounding collection mimicking adjacent\nbowel loops ().', '.']","Figure 2. Day eight post op CT ordered for investigation of post-operative sepsis( ) Axial ( ) Coronal planes. A large infectedhaematoma is seen within the right iliac fossa (blue arrow). A retained,migrated appendicolith (white arrow) can be seen within the left anteriorabdomen with a small surrounding collection (red arrows). This was notdetected at the time. The collection is noted to appear similar to closelyadjacent bowel loops ( green arrow), which may havemade detection more difficult, particularly given relative paucity ofintra-abdominal fat.",yes
PMC9332126,Figure_3,oa_package/f7/a0/PMC9332126.tar.gz,"['Our data confirm that treatment with tunicamycin induced ER stress in both LNCaP and CWR22Rv1 cells, as shown by an increase in GRP78 protein levels (D,E).', 'Similarly, we confirmed that thapsigargin induced ER stress in our model, shown through an increase in GRP78 protein levels (F,G).', 'We also detected an increase in EDEM3 in response to thapsigargin exposure (F,G).', 'EDEM3 is associated with ER stress in prostate cancer patients and is ER-stress responsive.']","Figure 3 EDEM3 is associated with ER stress in prostate cancer patients and is ER-stress responsive. ( ) Spearman correlation of EDEM3 gene expression with the three UPR stress sensors ERN1 (IRE-1), EIF2AK3 (PERK) and ATF6. Gene expression values are a part of TCGA prostate adenocarcinoma (PRAD) cohort, accessed through CBioPortal. n = 493 [ ]. mRNA expression values for EDEM3 were correlated with ERN1, EIF2AK3 and ATF6. values were calculated using a two-tailed Spearman correlation with 95% confidence intervals. **** < 0.0001. ( ) Western blot analysis of EDEM3 and GRP78 in LNCaP cells in response to 2.5 g/mL tunicamycin for 24 h. tubulin was used as a loading control. ( ) Western blot analysis of EDEM3 and GRP78 in CWR22Rv1 cells in response to 2.5 g/mL tunicamycin for 24 h. tubulin was used as a loading control. ( ) Western blot analysis of EDEM3 and GRP78 in LNCaP cells in response to 100 nM thapsigargin for 24 h. actin was used as a loading control. ( ) Western blot analysis of EDEM3 and GRP78 in CWR22Rv1 cells in response to 100 nM thapsigargin for 24 h. actin was used as a loading control.",yes
PMC3575255,Figure_6,oa_package/e1/a4/PMC3575255.tar.gz,"['Monomeric Abeta was preferentially accumulated in isolated synaptosomal fraction, from aged PS1/APP mice, whereas APP-C99 fragment preferentially localized in microsomes.']","Figure 6 ) Microsomal and synaptosomal fractions were isolated from 6, 12 and 18 months WT and PS1/APP mice. Using these samples, we tested the distribution of LC3-II autophagosomal marker (a1), the APP-CTF fragments (a2) and the monomeric Abeta peptide (a3 and a4 for a longer exposure). ATP-synthase and b-actin were used as control of synapsomal integrity and protein loading. Quantitative analysis of western blots demonstrated the age-dependent accumulation of LC3-II (compared with the respective fraction in 6 month WT mice) ( ) and C99 (as compared with 6-month-old PS1/APP microsomes) ( ) in microsomal fractions. The monomeric Abeta was preferentially accumulated in the synaptosomal fractions ( ). These experiments were repeated four times. ) Control experiments demonstrating the intrasynaptosomal origin of the monomeric Abeta. The tissue from a 12 month-old PS1/APP mice was divided, homogenized in absence (control) or in presence of 0.5% CHAPS + 0.5% DOC, and synaptosomes were isolated by Ficoll gradients. As shown, the disruption of cell membranes avoided the Abeta flotation on gradients (upper panels). On the other hand, tissue from 6 month-old WT mice were homogenized in absence (minus) of presence (plus) of synthetic Abeta42 and the synaptosomes were isolated. No Abeta were compartmentalized during homogenization.",yes
PMC7571779,Figure_3,oa_package/6a/f0/PMC7571779.tar.gz,"['Histopathological images of the intradural epidermoid tumor A) Squamous epithelial cyst containing abundant keratin (H E stain, original magnification: 100X).']","Figure 3 Histopathological images of the intradural epidermoid tumor A) Squamous epithelial cyst containing abundant keratin (H&E stain, original magnification: 100X). B) Squamous epithelium lacks skin adnexal structures and shows no cytologic atypia (H&E stain, original magnification: 200X).",yes
PMC8525073,Figure_2,oa_package/bc/c1/PMC8525073.tar.gz,[],FIGURE 2 Patient selection and study schema,yes
PMC3084338,Figure_5,oa_package/d9/a5/PMC3084338.tar.gz,"['Similarly, claudin-5 was diffusely expressed in the endothelial lining of unaffected dog arteries and throughout most of the endothelium lining vessels from affected dogs, except in areas of plaque formation where it was absent or highly discontinuous ().', 'Endothelial claudin-5 expression in an untreated MPS-I affected dog is almost completely lost over intimal plaques (arrow), in comparison to adjacent arterial wall, were no plaque is present (insets; claudin-5 IHC stain).']","Figure 5 Endothelial claudin-5 expression in an untreated MPS-I affected dog is almost completely lost over intimal plaques (arrow), in comparison to adjacent arterial wall, were no plaque is present (insets; claudin-5 IHC stain).",yes
PMC4501135,Figure_5,oa_package/7c/84/PMC4501135.tar.gz,"['Myopic foveoschisis is imaged despite the longer axial lengthMacular holeIn cases of the macular hole, while initiating the ILM peeling, surgeons are often concerned about inadvertent nerve fiber layer touch or breaks, which may lead to positive scotomas.']",Figure 5 Myopic foveoschisis is imaged despite the longer axial length,yes
PMC5409378,Figure_3,oa_package/6f/f6/PMC5409378.tar.gz,"['Histopathology examination revealed angioblastic meningioma with necrosis and hemorrhagic centers inside the tumor [].', '1 Hematoma; 2 MassAngiomatous meningioma A meningioma in which many large and small vascular channels predominate, with small intervening nests of meningotheliomatous or fibrous meningioma (three small arrows), hematoma on stroma (long arrow)Postoperative courseControl brain CT Scans confirmed complete removal of the lesion as shown in b, and the patient was discharged from hospital on the 7th postoperative day.']","Figure 3 Angiomatous meningioma A meningioma in which many large and small vascular channels predominate, with small intervening nests of meningotheliomatous or fibrous meningioma (three small arrows), hematoma on stroma (long arrow)",yes
PMC3192513,Figure_2,oa_package/ef/bf/PMC3192513.tar.gz,['The patient was noted to have asymmetrically and atypically thin pedicles at these levels [].'],"Figure 2 (Top) axial CT and T2 MRI at the level of L1 showing bilateral pedicle fractures and subluxation associated with canal compromise. (Middle) axial CT and T2 MRI at the level of L2 showing bilateral pedicle fractures and a dorsally located extradural meningeal cyst. (Bottom) axial CT and T2 MRI at the level of L3 showing aberrantly thin pedicles, anterior displacement of the neural elements, and dorsally located extradural meningeal cyst",yes
PMC3278928,Figure_2,oa_package/5f/d4/PMC3278928.tar.gz,"['Different from the prokaryotic cells, eukaryotic cells were shown to have a variety of small RNAs in their nuclei ().', 'The oligonucleotide peak from the GTP region was digested with snake venom phosphodiesterase and separated by electrophoresis in the first dimension followed by chromatography on second dimension (2).', 'The peak from the GTP region in 1 digested with RNase P1 produced pUm, pA (peak a in 3), and cap core m3\n2,2,7GpppAm (peak b in 3).', ' Table 10 shows the radioactivity distribution in peaks a and b in 3.', 'For the analysis of a number of phosphates in cap core (peak b), the cap core was treated with NaIO4 and aniline to remove m3\n2,2,7G by -elimination reaction (4).', 'Um and Am migrated through the butanol-boric acid while the m3\n2,2,7G and C, which form complexes with borate, were retarded in the butanol-boric acid phase (5).', 'The tritium-labeled compound was identified as a trialcohol derivative of m3\n2,2,7G (6).', 'Oligonucleotides were subsequently digested with snake venom phosphodiesterase, and the resulting 5 nucleotides were separated first by electrophoresis and second by chromatography (7).', 'The remaining oligonucleotide did not have m3\n2,2,7G, indicating that the m3\n2,2,7G is linked by pyrophosphate linkage (8).', 'The [32P]-labeled U3 RNA digested with T2 and U2 RNA produced 2 spots that were separated by two-dimensional electrophoresis (9).', '[32P] AnalysisThe [32P]-labeled U3 RNA digested by T1 RNase and U2 RNase was separated by two-dimensional electrophoresis (9).', '001""/> Polyacrylamide gel electrophoretic separations of nuclear 4 7S RNAs of rat liver and Novikoff hepatoma cells [7].', '021""/>2Nucleotide composition of U1 5 oligonucleotide [16].', '022""/>3Cap core chromatography [16].', '023""/>4The -elimination of cap core [16].', 'The cap core (peak b from 3) was treated with NaIO4 and aniline to remove m3\n2,2,7G by -elimination reaction.', '024""/>5Two-dimensional chromatography in borate system [12].', '025""/>6Fluorograph of [3H]-labeled trialcohol derivatives of m3\n2,2,7G from U2 RNA 5 end [12].', '026""/>7Autoradiograph of nucleotides from [32P]-labeled U2 RNA 5 fragment [12].', '027""/>8The susceptibility of 5 cap to pyrophosphatase [12].', '028""/>92D map of U3 RNA digest [58].', 'The 5 fragment from 9 was eluted and treated with alkaline phosphatase and chromatographed on DEAE-Sephadex A-25 with standards of GMP, GDP, and GTP.', 'The products were separated on a DEAE column (3).', 'The products were separated as in 7.']","Figure 2 Polyacrylamide gel electrophoretic separations of nuclear 47S RNAs of rat liver and Novikoff hepatoma cells [ ]. (a) The 8% gel electrophoretic patterns of 47S RNA from various cell organelles of rat liver. The gel was stained with methylene blue for RNA visualization. (1) Nuclear 47S RNA, (2) ribosomal 47S RNA, (3) mitochondrial 47S RNA, and (4) soluble cytoplasmic sap 47S RNA. (b) The 10% slab polyacrylamide gel electrophoretic separation of [ P]-labeled 47S RNA from Novikoff hepatoma cell nuclei. The gel was autographed with X-ray film.",yes
PMC8225372,Figure_1,oa_package/d7/6a/PMC8225372.tar.gz,['.'],"Fig. 1. Universal bed adaptor and how it was used during the simulation that led to the identification of latent safety threats. A, Universal bed adapter (PDSA1) round 1. B, LST identified with the Universal bed adapter.",yes
PMC10712447,Figure_3,oa_package/1f/75/PMC10712447.tar.gz,[],"Figure3 Transthoracic echocardiography and three-dimensional transoesophageal echocardiography showing a cardiac mass (arrow) on the epicardial free wall of the left atrium and ventricle ( , ). Colour Doppler mid-oesophageal view showing the feeding vessels in the mass ( ).",yes
PMC7149979,Figure_3,oa_package/55/dc/PMC7149979.tar.gz,[],"Fig. 19.3 Macroscopic appearance of multiple sclerosis in the brainstem. Chronic multiple sclerosis lesions (arrows) in the pons ( ) and medulla oblongata ( ) of a 47-year-old female with an 18-year history. Sections through the formalin-fixed brain ( ) and corresponding histologic sections ( , luxol fast blue) show multiple well-demarcated demyelinated plaques (arrows).",yes
PMC5621804,Figure_10,oa_package/67/29/PMC5621804.tar.gz,[],"Fig. 10. Complete and partial tears of the ATFL. A. Long-axis ultrasonography of the ATFL shows hypoechoic discontinuity of the ligament, compatible with a complete tear (arrow). B. An inversion stress image provides more diagnostic confidence regarding the complete tear (arrows). C. Normal appearance of the ATFL (arrowheads) is shown. D. In another patient with lateral ankle pain, the ligament exhibits heterogeneous hypoechogenicity with some calcifications. This finding reveals a chronic partial tear (arrow). ATFL, anterior talofibular ligament; LM, lateral malleolus; Ta, talus.",yes
PMC3216603,Figure_2,oa_package/61/f7/PMC3216603.tar.gz,"[' shows typical results in a normal mouse, starting from the entire cerebellum and then focusing down to local dendrites.', 'The progressive zoom (box in A) clearly reveals the 4-layer arrangement (', '2B) and the network of different types of neurons and glias (C), as well as finely detailed PC dendrites (', 'In addition to the projection images, we performed microtomography of the cerebellar tissue (the yellow box in the C) and could observe in 3-D the realistic neuronal network (', 'org/1999/xlink"" xlink:href=""srep00122-f1""/>Microradiograph of a normal mouse cerebellum.', '(A) 3-D network of cerebellar neurons (the yellow box in the C).']","Figure 2 Microradiograph of a normal mouse cerebellum. (A) Patched image of the entire cerebellum (300 m). The dashed line marks one lobule, consistent with the scheme in (B). (B) Lobule scheme. PC, Purkinje cell; BG, Bergmann glial cell; GC, granule cell; note the 4 layers: 1, molecular layer; 2, PC layer; 3, granular layer; 4, white matter. (C) Magnified image of the box region in (A) (red, orange, and green arrowheads: Purkinje, Bergmann glia, and granule cells). (D) Magnified image of the black box region in (C). (Scale bars: A, 600 m; C, 300 m; D, 50 m.)",yes
PMC8285324,Figure_2,oa_package/17/eb/PMC8285324.tar.gz,"['2) [16 ].', 'Representation of two paradigmatic cases of pancreatic neuroendocrine tumors, one with retained expression of DAXX/ATRX at immunohistochemistry (A hematoxylin-eosin, B DAXX immunostaining, C ATRX immunostaining; original magnification 10) and one with DAXX loss (D hematoxylin-eosin, E DAXX immunostaining, highlighting loss of nuclear expression; original magnification 20)ALT Is a Strong Prognostic Indicator in PanNETIn PanNET, the first description of the poor prognostic role of the activated ALT mechanism has been provided by Marinoni and colleagues that analyzed both cancer-specific and disease-free survival [14 ].']","Fig. 2 Representation of two paradigmatic cases of pancreatic neuroendocrine tumors, one with retained expression of DAXX/ATRX at immunohistochemistry ( hematoxylin-eosin, DAXX immunostaining, ATRX immunostaining; original magnification 10) and one with DAXX loss ( hematoxylin-eosin, DAXX immunostaining, highlighting loss of nuclear expression; original magnification 20)",yes
PMC7470929,Figure_4,oa_package/e6/4a/PMC7470929.tar.gz,"['Hippocampal and cortical neurons (; data not shown) prepared from postnatal Sncawt/GFP and SncaGPF/GFP mice developed normally in culture, with aSyn-GFP detectable by DIV7.', 'aSyn-GFP was mainly enriched in vesicles (A), similar to what is seen in Snca-GFP brains.', 'As previously reported for wt aSyn, aSyn-GFP was also present in the cell body during early developmental stages (Extended Data -1).', 'In mature cultures, aSyn-GFP colocalized strongly with Synapsin 1, a marker of synaptic vesicles (A).', 'In addition, aSyn and aSyn-GFP strongly overlap in Sncawt/GFP neurons (B).', '.', 'Immunocytochemistry showing distribution of aSyn in wt neurons is shown in Extended Data -1.', 'Complete blot is shown in Extended Data -2.', 'Comparison of aSyn and Synapsin 1 redistribution by immunostaining is shown in Extended Data -3.', '10.', 'f4-1Extended Data -1Mature Sncawt/wt primary hippocampal neurons were stained with the same anti-aSyn antibody as in (9027).', 'tif"" content-type=""local-data"">-1, TIF file.', '10.', 'f4-2Extended Data -2Full 9027 Western blotting for C.', 'tif"" content-type=""local-data"">-2, TIF file.', '10.', 'f4-3Extended Data -3Sncawt/GFP neurons were fixed before, after a 2-min exposure to 90 mm KCl, or after a 15-min recovery from HK stimulation and co-stained with an anti-Synapsin 1 antibody.', 'tif"" content-type=""local-data"">-3, TIF file.', 'Immunoblot analysis of hippocampal culture lysates with aSyn and GFP antibodies confirmed the expression of a single major species consistent with intact aSyn-GFP at the expected ratios with respect to the wt protein in each genotype (C; Extended Data ', 'Addition of KCl induced a marked decrease in aSyn-GFP intensity in individual vesicles within 2 min of treatment (D,E), in agreement with the dynamics previously reported for wt aSyn (Fortin et al.', 'Unlike Synapsin 1, aSyn-GFP did not fully re-establish its vesicular localization after a 15-min recovery period following KCl treatment (Extended Data -3).', ', participation in synaptic vesicle cycling) as tagged aSyn disperses after synaptic stimulation (), in agreement with previous studies (Fortin et al.', ' panel B demonstrates significant AS and GFP in the cell body that in intensity not differs very much from the presynaptic signals.']","Figure 4. Expression, subcellular localization and synaptic vesicle cycle of aSyn-GFP in primary neurons. Primary hippocampal neurons were derived from newborn pups and kept in culture for 1421d. , Co-localization of aSyn-GFP with the presynaptic marker Synapsin 1. , Co-localization between endogenous GFP signal and an anti-aSyn antibody (9027) in neurons. Immunocytochemistry showing distribution of aSyn in wt neurons is shown in Extended Data . , Western blotting of lysates from neurons of the indicated genotypes using antibodies against aSyn, GFP, and GAPDH. Complete blot is shown in Extended Data . , Heterozygous primary hippocampal neurons were imaged before and after stimulation with 90 m KCl. , Quantification of fluorescence intensity of GFP positive vesicles before (F0) and following (F1) treatment with control (KRH, Krebs Ringer HEPES) or stimulation buffer (HK, 90 m KCl). The data are expressed as the F1/F0 ratio (mean SEM from two subfields per dish, >70 vesicles per dish, =34 dishes from two to three independent cultures). Asterisks indicate statistical significance ( = 0.0061) as determined by two-tailed unpaired Students test with Welchs correction. Comparison of aSyn and Synapsin 1 redistribution by immunostaining is shown in Extended Data . Scale bars: 20m ( , ), 10m (crop), 10m ( ).",yes
PMC10371829,Figure_6,oa_package/23/56/PMC10371829.tar.gz,"['73,80,81 Significantly decreased intensities of GFAP, a marker for astrocytes, and CD68, a marker for activated microglia, were observed in spinal cord sections of Tmem106b / Grn / mice treated with AAV1- or AAV9-GRN compared to mice injected with buffer control (s 6A and 6B).', 'Analysis of microglial morphology in the spinal cord revealed a significant increase in the number of microglia processes in AAV1- or AAV9-GRN treated Tmem106b / Grn / mice (s 6C and 6D), indicating a reduced microglia activation with restored PGRN expression.', ' 6AAV1/9-mediated PGRN expression partially ameliorates glia activation in the spinal cord of Tmem106b / Grn / mice(A and B) Representative fluorescence images of spinal cord sections (C1-C4) from 5-month-old WT or Tmem106b / Grn / mice injected with buffer control or AAV1/9-GRN viruses at 6 weeks of age were stained with GFAP or CD68 antibodies (A), and the intensity of GFAP or CD68 were quantified (B).']","Figure5 AAV1/9-mediated PGRN expression partially ameliorates neuronal loss in the spinal cord of mice Representative images of spinal cord sections (C1-C4) from 5-month-old WT or mice injected with buffer control or AAV- viruses at 6weeks of age were stained with NeuN (A)or ChAT (C)antibodies. The numbers of NeuN- and ChAT-positive neurons were quantified (B, D). 3 sections/mouse and 36 mice were analyzed for quantification. Data are presented as mean SEM. One-way ANOVA tests with Tukeys multiple comparisons: , p<0.05, , p<0.01, , p<0.001, , p<0.0001; ns, no significance. Scale bar: 100m (A); 20m (C).",yes
PMC11328004,Figure_362,oa_package/e2/f1/PMC11328004.tar.gz,[],Chart 299 Structures of Complexes,yes
PMC4879631,Figure_4,oa_package/61/92/PMC4879631.tar.gz,"['Importantly, memory deficits were observed in conjunction with increased microRNA-146a levels in the Vehicle group compared to Control mice (A,B).', 'The 5xFAD mice treated with antagomir had a higher percentage of spontaneous alternations in the Y-maze, a higher percentage of time spent in the correct quadrant in Morris water maze and a shorter escape latency compared to Vehicle treated 5xFAD mice, indicating that antagomir increased hippocampal memory function (A).', 'Successful inhibition of microRNA-146a was achieved in the hippocampus following bilateral intrahippocampal injections of the microRNA-146a antagomir, while the levels of microRNA-146a in prefrontal cortex remained unchanged (B).', 'The levels of ROCK1 protein and phospho-PTEN protein in the hippocampus but not prefrontal cortex were rescued in 5xFAD mice treated with intrahippocampal microRNA-146a antagomir compared to Vehicle treated 5xFAD mice, and a reduction in phospho-tau was observed (C,D).', 'org/1999/xlink"" xlink:href=""srep26697-f3""/>Intra-hippocampal delivery of antagomir.']","Figure 4 Intra-hippocampal delivery of antagomir. ( ) The Y maze revealed that the percentage of spontaneous alternations was higher in 5xFAD mice treated with antagomir compared with Vehicle treated 5xFAD mice; and the Morris water maze showed shorter escape latency and higher percentage of time spent in the correct quadrant for 5xFAD mice treated with antagomir compared to Vehicle treated 5xFAD mice. ( ) The relative expression of microRNA-146a (miR146a) was significantly inhibited in hippocampus, but not prefrontal cortex, after bilateral intra-hippocampal injections ( =4). ( ) The hippocampal protein levels of the related signaling pathway markers (ROCK1-pPTEN-pTau) were evaluated by Western blotting. A representative gel image is shown from four Control (wild-type), four Vehicle treated 5xFAD, and four 5xFAD with antagomir mice. ( ) The prefrontal protein levels of the related signaling pathways (ROCK1-pPTEN-pTau) were evaluated by Western blotting. A representative gel image is shown from four Control (wild-type), four Vehicle treated 5xFAD, and four 5xFAD mice treated with antagomir mice. The data are shown as meanSE.",yes
PMC3662058,Figure_3,oa_package/39/a7/PMC3662058.tar.gz,['Photomicrograph of liver granulomas of S.'],"Fig. 3 Photomicrograph of liver granulomas of -infected vector-vaccinated control and infected SMALDO vaccinated groups.(A) Vaccinated control group: a large fibrocellular granuloma formed of a central egg surrounded by inflammatory cells and deposited collagen fibers. (B) IP, (C) SC, and (D) IM groups showing small cellular granulomas formed of a central egg, surrounded by lymphocytes, histiocytes, fibroblasts, and thin concentric collagen fibers (Masson's trichrome stain, 200).",yes
PMC8328700,Figure_2,oa_package/ba/6b/PMC8328700.tar.gz,"['Furthermore, the histopathological analysis detected noticeable abnormalities in neither the control nor the treatment groups (s 2 and 3).', '001""/>(a, b) Photomicrographs of liver sections of control rats; (c, d) liver sections of rats treated with 65 mg/kg of essential oil of Thymus schimperi; (e, f) liver sections of rats treated with 130 mg/kg of essential oil of Thymus schimperi; and (g, h) liver sections of rats treated with 260 mg/kg of essential oil of Thymus schimperi.']","Figure 2 (a, b) Photomicrographs of liver sections of control rats; (c, d) liver sections of rats treated with 65mg/kg of essential oil of ; (e, f) liver sections of rats treated with 130mg/kg of essential oil of ; and (g, h) liver sections of rats treated with 260mg/kg of essential oil of .",yes
PMC6134668,Figure_2,oa_package/50/32/PMC6134668.tar.gz,"['5 cm in diameter in the section of the wall, with slightly harder grey and white matter (a).', '.', '1177_0300060518776579-fig2""/>Histochemical staining confirmed a poorly differentiated squamous cell carcinoma, with multiple intravascular tumor emboli.', 'However, there was no cancer in the right iliac bone, right peritoneum of the pelvic wall, omental tissue or intraoperative washings (b).', 'Immunohistochemical analysis was positive for E-cadherin, Ki-67 (positive region 80%) (c) and mutant p53 (d), weakly positive for CK5/6, P63, CK7 and CK20, and negative for P40, HPV16, HPV18 and HPV31.']","Figure 2. Postoperative pathological diagnosis. (a) Gross features of the uterus. (b) Histopathology of ascites. Hematoxylineosin staining, 100. (c) Ki-67 immunohistochemistry (positive region 80%). 3,3-Diaminobenzidine staining, 400. (d) p53 immunohistochemistry. 3,3-Diaminobenzidine staining, 400.",yes
PMC8659713,Figure_6,oa_package/98/8b/PMC8659713.tar.gz,"[', a significantly higher expression of TNF- and IFN- was observed in the SBP-1 KO infected group compared to the WT infected group, while CCL2 and CXCL10 expression remained the same (A 6D).', '(A 6D).', 'The expression of the anti-inflammatory molecules IL-10, ANXA1, DUSP1, GILZ and HO-1 was significantly increased upon infection (E 6I).', 'g006Resolution of inflammatory cytokine expression in lungs of SBP-1 KO infected mice.']",10.1371/journal.ppat.1010114.g006,yes
PMC8297414,Figure_5,oa_package/98/7a/PMC8297414.tar.gz,"['As shown in , no significant differences in trypsin or lipase activities were observed in either treatment group after 30 and 45 days (s 7A,B).']","FIGURE 5 Intestinal goblet cells observations of the largemouth bass intestine (AB-PAS staining) The number and distribution of intestinal goblet cells in fish fed the NC diet for 30 days; proliferation of goblet cells (pentagram), increased mucus secretion (triangles) of fish fed the HC diet for 30 days; number and distribution of intestinal goblet cells in NC-fed fish after 45 days; , the number of goblet cells decreased in HC-fed fish at 45 days, and a large amount of mucus was secreted into the intestinal lumen (triangle); number of intestinal goblet cells in largemouth bass fed the NC and HC diets for 30 and 45 days (*** < 0.001. NC: 0% -starch diet, FC: 22% -starch diet).",yes
PMC4878199,Figure_7,oa_package/31/dd/PMC4878199.tar.gz,['Polymorphism at position 82 of Tlr2 modulates severity and course of EAE.'],"Figure 7 . EAE score was evaluated as described in Section . In all panels, data report the cumulated results of two independent experiments each comprising comparable numbers of mice from the genetic backgrounds examined in each panel; mice were evaluated daily for 60days in a blind fashion with respect to genotype. Closed symbols and bars report data from F1 (SJLB6 ) mice; the open symbols and bars report data from F1 (SJLB6 ) mice. The average disease score in 12 F1 (SJLB6 ) and 17 F1 (SJLB6 ) mice immunized with p139 without treatment with PTx. The average disease score in 10 F1 (SJLB6 ) and 18 F1 (SJLB6 ) mice immunized with p139 and treated with PTx at day 0 and day 3 after immunization. Total score of the diseases described in are reported as boxplot. Maximal decrease of disease score between day 20 and day 30 after immunization are reported as boxplot (two-tailed MannWhitney test, ** <0.01).",yes
PMC2956888,Figure_1,oa_package/82/cf/PMC2956888.tar.gz,"[' 1a inset, 2a).', '', 'Scale bar in inset in (a) 100 m', ' 1b, 2c, d).', ' 1a).', ' 1c, d).']","Fig.1 Cortex in case 1 (day27) in pSAP deficiency. Numerous cortical neurons with a rather normal architecture. Discrete neuronal storage accented in basal layers ( ); H&E. Strong neuronal perikaryal staining for cathepsin D. Paucity of microglial phagocytes demonstrated by CD 68 antibody (clone PGM1), and of astroglia stained with GFAP antibody. correspond to the identical cortical area. Scale bar 200m . Scale bar in inset in (a) 100m",yes
PMC6545524,Figure_3,oa_package/74/90/PMC6545524.tar.gz,"[""Adipose tissueAdipogenesis, osteogenesis, chondrogenesisCsaki et al[46], 2007Adipose tissueAdipogenesis, osteogenesis, neurogenesisKang et al[62], 2008Adipose tissueAdipogenesis, osteogenesis, chondrogenesisNeupane et al[68], 2008Adipose tissueAdipogenesis, osteogenesis, chondrogenesis, myogenesisVieira et al[48], 2010Adipose tissueAdipogenesis, osteogenesis, myogenesisMartinello et al[65], 2011Adipose tissueChondrogenesisReich et al[56], 2012Bone marrowAdipose tissueOsteogenesisKang et al[50], 2012Bone marrowUmbilical cordWharton's jellyAdipose tissueAdipogenesis, osteogenesisKisiel et al[52], 2012Bone marrowMusclePeriosteumAdipose tissueAdipogenesis, osteogenesisTakemitsu et al[64], 2012Bone marrowBone marrowChondrogenesis, cardiogenesisHodgkiss-Geere et al[120], 2012Synovial fluidChondrogenesisKrawetz et al[55], 2012Bone marrowAdipogenesis, osteogenesis, chondrogenesisVolk et al[59], 2012Adipose tissueAdipogenesis, osteogenesis, chondrogenesisGuercio et al[58], 2013Adipose tissueAdipogenesis, osteogenesis, chondrogenesisScreven et al[49], 2014Bone marrowBone marrowAdipogenesis, osteogenesis, chondrogenesisBertolo et al[60], 2015Adipose tissueOsteogenesis, chondrogenesisSullivan et al[118], 2016Bone marrowAdipose tissueAdipogenesis, osteogenesisRussell et al[57], 2016Bone marrowSynoviumAdipogenesis, osteogenesis, chondrogenesis, osteoclast differentiationWijekoon et al[54], 2017Adipose tissueAdipogenesis, osteogenesis, chondrogenesisBearden et al[53], 2017Bone marrowSynoviumBone marrowAdipogenesis, osteogenesis, chondrogenesis Hepatocyte and functional insulin-secreting cell differentiationZhang et al[47], 2018Adipose tissueAdipogenesis, osteogenesis, chondrogenesisSasaki et al[40], 2018Bone marrowSynoviumInfrapatellar fat padBone marrowChondrogenesisEndo et al[61], 2018Trilineage differentiation of canine mesenchymal stem cells.""]",Figure 3 Trilineage differentiation of canine mesenchymal stem cells. A: Adipogenic differentiation confirmed by oil red-o staining. Bar = 50 m; B: Osteogenic differentiation confirmed by alizarin red. Bar = 100 m; C: Chondrogenic differentiation confirmed by toluidine blue staining. Bar = 250 m.,yes
PMC10734879,Figure_2,oa_package/41/5a/PMC10734879.tar.gz,"['8-year-old male with inability to walk, flu-like symptoms, and increased serum CK level.']","Figure 2 8-year-old male with inability to walk, flu-like symptoms, and increased serum CK level. Strain elastography of the symptomatic right side reveals an elastography score of 5 (a), while contralateral side has a score of 3 (b). Also note the increase in muscle thickness and echogenicity on the gray scale ultrasound on the symptomatic right side when compared to contralateral.",yes
PMC10713242,Figure_6,oa_package/6b/47/PMC10713242.tar.gz,"['Nevertheless, an issue arises with cases showing overlapping morphology, such as PM-LBCL with fibrous bands separating the tumor into cellular nodules (mimicking NS-CHL), or with the syncytial variant of NS-CHL showing sheets of HRS cells with variable necrosis (mimicking PM-LBCL) ().', 'Diagnostic challenges of PM-LBCL and NS-CHL.']","Figure 6 Diagnostic challenges of PM-LBCL and NS-CHL. (A) PM-LBCL can have bands of fibrosis separating the tumor cells into nodules resembling NS-CHL at low power (H&E, 20). (B) Other cases have a polymorphic background admixed with large lymphoma cells also mimicking NS-CHL (H&E, 100). (C,D) The syncytial variant of NS-CHL is composed of sheets of large lymphoma cells indistinguishable from LBCL (H&E, 200 and 400, respectively). (E,F) Mediastinal lesions tend to have marked sclerosis that can limit interpretation without the use of immunohistochemical stains. (E) NS-CHL with fibrosis (H&E, 200), (F) PM-LBCL with abundant sclerosis (H&E, 200). PM-LBCL, primary mediastinal (thymic) large B-cell lymphoma; NS-CHL, nodular sclerosis classic Hodgkin lymphoma; H&E, hematoxylin and eosin; LBCL, large B-cell lymphoma.",yes
PMC7643305,Figure_3,oa_package/ff/05/PMC7643305.tar.gz,"[' 3a).', ' 3b).', 'DMR7 and SKT82 bind to discontinuous epitopes of tau.', 'b Schematic of tau constructs and tau mAb bindingTau mAbs inhibit seeded fibrillization of endogenous tau in primary neuronsTo determine whether DMR7 and SKT82 binding to AD-tau would inhibit the seeding of tau aggregates in primary neurons in a previously described assay [21], WT mouse cortical neurons were treated with AD-tau to template fibrillization of endogenous cellular mouse tau into insoluble aggregates.']","Fig. 3 DMR7 and SKT82 bind to discontinuous epitopes of tau. Western blot of tau fragments with DMR7 and SKT82 reveal distinct partial binding patterns to tau fragments. DMR7 and SKT82 detect full length tau isoforms T44, T43, and T44, but not the microtubule binding domain, K18. Loss of the C-terminus in the ABP construct and loss of the proline rich domain in the K18-P construct reduce binding of DMR7 and SKT82, demonstrating a proline-rich domain and c-terminal epitope. Equal loading of tau protein fragments was determined by Coomassie blue stained gel and K9JA total tau antibody, which does not detect the ABP fragment. Schematic of tau constructs and tau mAb binding",yes
PMC7587296,Figure_21,oa_package/a3/c2/PMC7587296.tar.gz,[],"Figure 12a. Mixed chest CT findings in an 86-year-old man. Axialnonenhanced CT image (lung window) obtained at hospital admission showssublobar consolidation (arrow) in the posterior segment of the right upperlobe, a finding more consistent with lobar pneumonia than COVID-19. Axial CT image obtained at a more superior level showsthe presence of ground-glass opacities (arrows). Altogether, the findingswere classified as indeterminate for COVID-19 pneumonia, according to theRSNA chest CT classification system ( ). The RT-PCR test results were positive for SARS-CoV-2.",yes
PMC9153318,Figure_4,oa_package/8e/ce/PMC9153318.tar.gz,"['006, respectively) [Table 9, ].', '000STSG in vitiligo patch on malleoli: (A) Pretreatment and (B) posttreatment at 20 weeksDISCUSSIONDespite the limitations and some side effects, surgical modalities appear to be the method of choice in recalcitrant stable vitiligo.']",Figure 4 STSG in vitiligo patch on malleoli: (A) Pretreatment and (B) posttreatment at 20 weeks,yes
PMC4365874,Figure_2,oa_package/04/2e/PMC4365874.tar.gz,[],"Fig 2 Characterization of P2X7 protein detection specificity. (A) Representative image of Western blot analysis with P2X7 antibody (Synaptic Systems) in protein samples from HEK cells overexpressing P2X7R, in brains and TA muscles from mouse and from two P2X7 knockout strains (Glaxo and Pfizer). Distinct bands of 78 kD are visible in protein samples from cells overexpressing P2X7R and in brain and muscle from mouse but not in knockout samples. The specificity of smaller bands is unclear. (B) PNGase F deglycosylation reduced the main band size to 65 kD in both P2X7R transfected HEK cells and in dystrophic myoblasts; = 3.",yes
PMC5728927,Figure_2,oa_package/19/5a/PMC5728927.tar.gz,['Intraoperative images of left basal interhemispheric approach before (A) and after (B) total resection of the tumor.'],"Figure 2 Intraoperative images of left basal interhemispheric approach before (A) and after (B) total resection of the tumor. 1, Olfactory nerve; 2, optic nerve; 3, tumor; 4, internal carotid artery; 5, anterior cerebral artery; 6, oculomotor nerve. All the vessels and nerves were perfectly preserved in the surgery.",yes
PMC4212118,Figure_5,oa_package/05/4a/PMC4212118.tar.gz,"['Upon MK801 injection, non-transgenic mice showed no indication of hypersynchronicity ( 5A,C and D).', 'In contrast, MK801 injection resulted in increased occurrence of hypersynchronicity in APP23 mice ( 5B, C and D; spike trains per 120 min: n = 5, t = 2.', 'Periodograms show MK801 treatment-induced power and frequency shifts in theta frequencies ( 5A and B).', 'In-depth spectral analysis revealed that, similar to non-transgenic recordings, MK801 induced strong increases in power of theta oscillations in APP23 spectra ( 5E,F; n = 5 animals 8-12 measurements each, F3,186 = 67.', 'Power of gamma oscillations was suppressed to similar extents by MK801 in APP23 and non-transgenic mice ( 5G,H; n = 5 animals 8-12 measurements each, F3,186 = 20.', 'However, strength of gamma oscillations remained still higher in APP23 spectra compared with non-transgenic spectra after MK801 injections ( 5G,H).', 'Calculation of the ratio of spectral power pre and post injection of MK801 in non-transgenic and APP23 recordings showed that MK801 had a significant effect on theta power ratio in APP23 mice ( 5I n = 5 animals 8-12 measurements each, t = 2.', '0175, Student s t-test), yet did not affect gamma power ratios ( 5J n = 5 animals 8-12 measurements each, t = 0.', 'CFC impairments in APP23 mice persisted before and after MK801 injections and coupling strength was not affected by MK801 ( 5K; n = 5 animals 2-3 measurements each, F3,23 = 5.', '\nNMDA receptor inhibition enhances hippocampal spike activity in APP23 mice.', 'MK801 inhibits p38 MAP kinase activity in the APP23 hippocampusPathways downstream of NMDA receptors in hippocampal neurons have been implicated in neurotoxicity, hypersynchronicity and pathology in AD mouse models including the APP23 transgenic mice [2,49].', 'Similar to MK801 ( 5I), SB203580 treatment did not impact on interictal CFC strength in APP23 and non-transgenic mice ( 6L; n = 5 animals 3-4 measurements each, F3,54 = 7.']","Figure 5 Representative EEG trace (LFP) and periodogram (0-60Hz) of non-transgenic and APP23 transgenic mice before and after injection of MK801 (0.4mg/kgi.p.). magnification of EEG traces marked in an by dashed box. (n=5) Spike train statistics for APP23 and non-transgenic (non-tg) recordings after injection of MK801. (n=5) Means.e.m.; ND, none detected Spectral power analysis of APP23 and non-transgenic (non-tg) recordings before and 20minutes after injection of MK801. Dashed box marks theta band. Meanss.e.m. (n=5). Quantification of spectra in . AUC, area under curve. Meanss.e.m. ****p<0.001 ***p<0.005 **p<0.01 *p<0.05 (ANOVA) Spectral power of EEG waves in APP23 transgenic mice and non-transgenic (non-tg) controls before and 20minutes after injection of MK801. Dashed box marks gamma band. Meanss.e.m. (n=5). Quantification of spectra in . AUC, area under curve. Meanss.e.m. **p<0.01 (ANOVA) Ratio of theta power pre/post-injection of MK801. (n=5) meanss.e.m. * p<0.05 (Students t-test) Ratio of gamma power pre/post-injection of MK801. (n=5) meanss.e.m. not significant (Students t-test) Modulation index before and 20minutes after injection of MK801. (n=5) meanss.e.m. **p<0.01 *p<0.05ns, not significant (ANOVA).",yes
PMC4362133,Figure_1,oa_package/0f/2d/PMC4362133.tar.gz,"['7 10 14 cm in size ().', 'An image showing a sagittal view of MRI T2 high signal intensity of the mass in the pelvis.']",Figure 1 An image showing a sagittal view of MRI T2 high signal intensity of the mass in the pelvis. The mass was compressing the rectum and urinary bladder.,yes
PMC5562695,Figure_1,oa_package/be/9f/PMC5562695.tar.gz,"[' 1 illustrates the challenges of relying solely on radiology readers to determine ground truth for disease extent using imaging alone.', 'Illustration of a pair of corresponding in vivo prostate MRI (c) and ex vivo histology images (b).', '\nConsequently, there has been a recent appreciation of the need to identify alternative image representations that can complement intensity information which includes image gradients2, co-occurrence information3, and image segmentations4 for the purposes of multi-modal image co-registration.', '( 1) The shape of the excised prostatectomy specimen tends to substantially change during the process of tissue fixation, slicing and sectioning.']","Figure 1 Illustration of a pair of corresponding prostate MRI ( ) and histology images ( ). On panel one can observe the cancer annotations made by two radiologists (green and red) unblinded to the corresponding histology images. The divergent annotations made by the two radiology readers, in spite of having access to the pathology images, suggests the need for accurate co-registration of pathology and radiology imaging in order to ascertain the ground truth extent for disease on radiology imaging (( ) the overlap registration visualization).",yes
PMC6717619,Figure_7,oa_package/fe/ef/PMC6717619.tar.gz,['Allergic bronchopulmonary aspergillosis (ABPA) bears mention due to the characteristic imaging appearance and need for specific medical management (figure 7).'],"Figure7 A 71-year-old male with a history of asthma presented with a 3-month history of productive cough. This patient had a positive sputum culture for and peripheral blood eosinophilia. Skin-prick test was positive for and serum IgE levels, including -specific IgE levels, were markedly elevated. a) Non-contrast-enhanced CT showshigh-attenuation mucus plugging (>70HU) within dilated central airways (arrow) and atelectasis typical for ABPA. b) Follow-up imaging after antifungal and steroid treatment revealed resolution of the mucus plugging and atelectasis with residual cylindrical bronchiectasis (arrow).",yes
PMC6919407,Figure_3,oa_package/8f/dc/PMC6919407.tar.gz,"['Histopathological examination of the acrometastases: (A) adenocarcinoma infiltration in the nail bed (H E), (B) tumor proliferation with acinar, tubular, and micropapilary pattern (H E, 100), (C) TTF1 100 diffusely positive (nuclear markers).']","Figure 3 Histopathological examination of the acrometastases: (A) adenocarcinoma infiltration in the nail bed (H&E), (B) tumor proliferation with acinar, tubular, and micropapilary pattern (H&E, 100), (C) TTF1 100 diffusely positive (nuclear markers).",yes
PMC4960960,Figure_3,oa_package/8f/81/PMC4960960.tar.gz,"['Alteration of CD8+ T-cells and memory CD8+ T-cellsAs shown in , compared to control (15.', 'Comparison of CD8+ T-cells (a) and memory CD8+ T-cells (b).']",Figure 3 Comparison of CD8 T-cells (a) and memory CD8 T-cells (b). CD8 T-cells and memory CD8 T-cells were sorted by flow cytometry as described in the methods. Vertical axes: percent of CD8 T-cells (%) or memory CD8 T-cells (%). Horizontal axes: Groups * < 0.05.,yes
PMC3497884,Figure_2,oa_package/a0/97/PMC3497884.tar.gz,[],Fig. (2) Magnetic resonance images: ) post-traumatic coronal MRimage of the knee (bone bruise in the lateral condyle); ) post-traumaticsagittal MR image of the knee (bone bruise in the lateralcondyle); ) coronal MR image of the knee obtained at the lastcontrol visit (after nine months); ) sagittal MR image of the kneeobtained at the last control visit (after nine months).,yes
PMC3056210,Figure_2,oa_package/fe/f1/PMC3056210.tar.gz,"['001""/>Abnormal qEEG in TBI patients.']","Figure 2 Abnormal qEEG in TBI patients. (a) Average normalized power spectrum representation of all TBI patients and controls that underwent qEEG. Note the significant increase in delta power and the decrease in the alpha band in TBI patients compared to controls (inset). (b) Power spectrum averages of patient subgroups according to the occurrence of seizures. While both the PTE and the non-epileptic group had elevated delta power compared to controls, only PTE patients had a significant reduction of alpha and increase of theta power. *= < .05.",yes
PMC3443272,Figure_8,oa_package/8a/fa/PMC3443272.tar.gz,"[' 8).', ' 8).', 'a A 3-month-old boy with a fever and reluctance to use his right limb.', 'e Axial T2 FS image confirms the fluid/abscess collection with extension outside the boneComputed tomographyCT is useful in the evaluation of bony destruction (', ' 8, 10 and 11) [19].', ' 8 and 9).']","Fig. 8 A 3-month-old boy with a fever and reluctance to use his right limb. Physical examination shows a swollen, red hip. Conventional imaging shows extensive destruction of the proximal right femur and swollen soft tissue. b Ultrasound of the right hip shows cortical destruction, the formation of a subperiostal fluid collection/abscess and infiltration of soft tissue. Coronal T1 STIR and axial PD images show cortical destruction and fluid collection/abscess formation in bone marrow. No contrast-enhanced imaging was performed in this case. Axial T2 FS image confirms the fluid/abscess collection with extension outside the bone",yes
PMC10070111,Figure_4,oa_package/ce/94/PMC10070111.tar.gz,"['As shown in A, IL13RA2 overexpression suppressed KF proliferation.', 'In addition, Western blot assay also showed that IL13RA2 overexpression decreased the expression of antiapoptotic proteins BCL2 and BCL2L1 (, B and C).', 'Importantly, p-STAT6 expression was decreased in KF-IL13RA2, demonstrating the role of IL-13RA2 in modulating p-STAT6 levels (D).', 'In addition, KF-IL13RA2 were less sensitive to AS1517499 but still more sensitive than NFs, as reflected by their IC50 values (, E and F).', 'Furthermore, Western blot assay confirmed that knockdown of IL13RA2 upregulated the expression of BCL2L1 and BCL2, mesenchymal markers (FN1 and -SMA), as well as COL3A1 (Supplemental ).', 'Overexpression of IL-13RA2 induced KF apoptosis.']","Figure 4 Overexpression of IL-13RA2 induced KF apoptosis. ( ) Growth curves of KFs upon IL-13RA2 overexpression. ( ) Annexin V/7-AAD double staining assay of KF-vector and KF-IL13RA2 cells ( = 5). Quantification of Annexin Vpositive rates, representing apoptotic rates, is shown below. ( and ) Expression of p-STAT6, BCL2L1, and BCL2 in KFs upon IL-13RA2 overexpression. Quantifications of relative gray scale is shown below the blots. ( and ) IC values of AS1517499 in KF-vector and KF-IL13RA2 cells. * < 0.05; ** < 0.01; *** < 0.001 by paired, 2-tailed Students test.",yes
PMC9700094,Figure_4,oa_package/5a/60/PMC9700094.tar.gz,"['New Approaches to Understand Aberrant Cell-Cell Interactions in Alzheimer s DiseaseRecently, we expanded upon existing Drosophila models of AD by creating a two-clone system to study the signaling interactions between A 42-expressing and WT neurons (; Yeates et al.', 'This system uses mitotic recombination by combining flippase/flippase recognition target (FLP/FRT) and the Gal4/UAS/Gal80 system to generate mosaics in developing retinal neurons (; Xu et al.', 'Both the A 42-expressing clone (GFP positive) and WT sister clone (GFP negative) are easily distinguished from the background, which weakly expresses GFP, facilitating quantification of the clone size ().', 'A two-clone system to study interactions between A 42-expressing and WT cell populations.', 'Indeed, in the absence of A 42, sister clones were not significantly different in size ().', 'However, in the presence of A 42 expressing clones, it was wild-type clones that were smaller suggesting a more complex interplay between A 42-expressing and WT cells ().', ', 2020; ).', 'Interactions between these clones lead to sensitization of WT cells and eventually cell death, resulting in smaller WT clones ().']","Figure 4 A two-clone system to study interactions between A -expressing and WT cell populations. (A) Generation of genetic mosaics in eye imaginal discs. By using FLP/FRT system in conjunction with the Gal4/UAS/Gal80 system and GFP expressed under an ubiquitin promoter, labeled clones can be generated (see Yeates et al., 2020). (B) Control clones. Mitotic recombination yields two sister clones: one with two copies of GFP, and one with no GFP expression. Both clones are identifiable against the background, which weakly expresses GFP. GFP-positive and GFP-negative clones were found to be equivalent in size. (C) A expressing experimental clones where GMR-Gal4 driver is used to drive expression of A in the developing retina. Mitotic recombination generates two sister clones: one is GFP-positive and expresses A . The other is GFP-negative and WT, expressing no A due to two copies of Gal4 repressor Gal80 (expressed under a tubulin promoter). Experimental data showed a surprising decrease in the size of WT clones adjacent to A -expressing clones. Evidence from DCP-1 (activated caspase) staining showed cell death in WT clones, suggesting that in this model, WT cells die first. A -expressing cells appeared to die later. JNK activation was observed in A -expressing clones. Blocking JNK activation in A -expressing clones prevented the death of WT cells. AD: Alzheimers disease; A : amyloid beta 42; FLP/FRT: flippase/flippase recognition target; GFP: green fluorescent protein; GMR: glass multiple repeat; JNK: c-Jun N-terminal kinase; WT: wild-type.",yes
PMC11426176,Figure_6,oa_package/f9/65/PMC11426176.tar.gz,"['All the myomatous nodules were totally removed (A, 6B).', '.']","Figure 6. The picture of case 2, including uterus, which has multiple leiomyomas ( ), and parasitic leiomyoma at right trocar site ( ).",yes
PMC6823752,Figure_5,oa_package/d5/d5/PMC6823752.tar.gz,"['A single loaded FiberTak (Arthrex) is inserted into the created hole (Fig 5).', 'The suture anchor is placed through the guide in the anterolateral portal at the superior aspect of the pectoralis major in this left shoulder that is abducted and externally rotated.']",Fig 5 The suture anchor is placed through the guide in the anterolateral portal at the superior aspect of the pectoralis major in this left shoulder that is abducted and externally rotated.,yes
PMC6099078,Figure_3,oa_package/76/6e/PMC6099078.tar.gz,['Representative histopathology images in the (A) cortex and (B) hippocampus regions of the rat brain (n = 3).'],"Figure 3 Representative histopathology images in the cortex and hippocampus regions of the rat brain ( = 3). Abbreviations: a, neuritic plaques and b, neurofibrillary tangles.",yes
PMC10274212,Figure_3,oa_package/6a/af/PMC10274212.tar.gz,"['After 4 months of receiving warfarin, the patient showed a complete resolution of LV thrombus ().', '.']",Figure 3. Contrast echocardiography 4-chamber view showing resolution of the apical thrombus. LV left ventricle.,yes
PMC9557836,Figure_6,oa_package/d1/70/PMC9557836.tar.gz,"['Moreover, the frequencies of endocrine hormone-positive cells within ILD and PDG compartments were not significantly altered in the presence of insulitis or pancreatitis ().', '.']","Figure 6. No significant difference in hormone-expressing endocrine cells in the ILD or PDG compartments of nondiabetes (ND) subjects with or without pancreatitis, or type 1 diabetes (T1D) subjects with insulitis as observed from 2 age-matched cohorts. Frequency of insulin+ (A), glucagon+ (B), somatostatin+ (C), and pancreatic polypeptide (PP)+ cells (D) in ILD and PDG. Data are presented as mean SEM for age-matched T1D insulitis+ donors (black open circles, n=10) and ND insulitis, pancreatitis donors (blue filled squares, n=10). Frequency of insulin+ (E), glucagon+ (F), somatostatin+ (G) and pancreatic polypeptide (PP)+ cells (H) in ILD and PDG. Data are presented as mean SEM for age-matched ND pancreatitis+ donors (blue open squares, n=12) and ND pancreatitis donors (blue filled squares, n=12). Each data point represents an average of 1 to 3 sections per donor (from the pancreatic head, body, and/or tail regions; see Table 1). Unpaired test, >0.05 all.",yes
PMC5552032,Figure_7,oa_package/44/b8/PMC5552032.tar.gz,"['g006""/>Preliminary histological findingsWe detected that both control and teriflunomide treatment arms exhibited demyelinated lesions in the corpus callosum ().', 'g007Solochrome staining TMEV induced demyelination in treated animals.']",10.1371/journal.pone.0182729.g007,yes
PMC4944637,Figure_1,oa_package/e0/02/PMC4944637.tar.gz,"['6 cm mass in the retroperitoneum with some extension into the left groin [].', '(a) Coronal view on computed tomography demonstrates a mass measuring 13.']",Figure 1 (a) Coronal view on computed tomography demonstrates a mass measuring 13.9 cm 14.6 cm 11.6 cm in the retroperitoneum. (b) Axial view on computed tomography demonstrates the mass and the Foley catheter shifted to the right pelvic region,yes
PMC8555694,Figure_3,oa_package/e7/32/PMC8555694.tar.gz,[],"Figure3 Full-field digital mammography. Craniocaudal position and mediolateral oblique position showed multiple high-density irregular masses, some of which were integrated with obscured septa, with multiple lobulations. Note the red arrow in panel A; the margin of the lesion was obscured by surrounding structures. Breast Imaging-Reporting and Data System (BI-RADS) 3.",yes
PMC10270617,Figure_2,oa_package/d2/eb/PMC10270617.tar.gz,"['ref024"" ref-type=""bibr"">24] ().', 'g002Locations of points and measurements in maxillary basal bone width.']",10.1371/journal.pone.0287343.g002,yes
PMC2213052,Figure_2,oa_package/c5/b5/PMC2213052.tar.gz,"[' shows that as was the case with the C57BL/6-Df1 / mice, restimulated lymphocytes from BALB/c-Daf1 / mice displayed increased IFN- and decreased IL-10 production as compared with those of WT control animals (', '.']","Figure 2. Responses of BALB/c WT and Daf1 mouse lymphocytes to antigen restimulation. Spleen and LN cells from each mouse were combined and restimulated with OVA in vitro (in triplicate assays) 12 d after immunization, and cytokine production was determined (each bar represents a single mouse, and four mice were used in each group). The x axis represents OVA concentration used for restimulation. Asterisks designate levels that were below the detection limits. Results are representative of two independent experiments.",yes
PMC8507321,Figure_5,oa_package/99/f3/PMC8507321.tar.gz,"[' 5f), the latter possibly originating in the basal nucleus of Meynert (BN).', ' Arrow in i points to extraneuronal neuromelanin lying free in the neuropil after severe neuronal lossPunch samplesFrom each case, including the negative human control, punch samples were collected (K.', ' 5e).', ' 5a and b.']","Fig. 5 Phospho-tau Histopathology (Part II). A 4mm punch from the IO of (male, 58years, NFT V) included portions of the immediately adjacent intermediate zone (IRZ) with strongly AT8-positive neurons and axons. In the IO itself, a single AT8-immunoreactive axon was detectable and some perivascular ARTAG was present, but none of the neurons there were AT8-immunoreactive. Tau seeding was below threshold (0.24). In at least two additional individuals ( and ), prominent AT8-positive neurons and axons in the IRZ accompanied by IO AT8-negative neurons as well as perivascular ARTAG may have accounted for the tau seeding signals in IO punches (2.29 and 4.74). Framed area in at higher magnification showing AT8-positive tau pathology at the punch edge. A 3mm punch with edges free of AT8 pathology from (female, 84years, NFT V), in which no portions of the IRZ were included. Framed area in at higher magnification displaying a clean punch edge and, directly beyond it, AT8-positive pathology in the IRZ. Tau seeding activity in the IO of this case was below threshold (0.08). AT8-positive axon ( ) in the substantia nigra, pars compacta (SNpc) of (female, 88years, NFT III). Some tau seeding in the SNpc was present in 13/20 individuals (0.8114.20), and was exceeded in the lower brainstem only by 15/20 cases in the LC (1.0019.43). point to AT8-positive axons in the GP of (female, 93years, NFT III) (see also Fig. g, h). The highest propensity for tau seeding (33.77) was detected in the basolateral subnucleus of the amygdala (AMY) of (female, 76years, Stage V), where severe diffuse neuronal AT8-pathology was accompanied by some AT8-immunopositive astrocytes (ARTAG). Tau seeding activity in the LC first became more pronounced during NFT stages III and V, e.g., 11.96 in (female, 93years, NFT III) ( ); 19.43 in (male, 76years, NFT III); and 5.71 in (male, 74years, NFT III) in micrograph . in points to extraneuronal neuromelanin lying free in the neuropil after severe neuronal loss",yes
PMC10158459,Figure_1,oa_package/61/28/PMC10158459.tar.gz,[],"FIGURE 1. Video capsule endoscopy images captured in stomach over time. Active bleeding noted as capsule approaches antrum (A), with erosion and ulcerated mucosa (B). Later, early clot formation noted in antrum with some fresh blood still present (C and D).",yes
PMC7702158,Figure_5,oa_package/c5/e9/PMC7702158.tar.gz,['Elevation of Adam10 and Notch2 promotes renal fibrosis in the CPF group.'],"Figure 5 Elevation of Adam10 and Notch2 promotes renal fibrosis in the CPF group. (A) Masson trichrome staining of kidneys sectioned at 6 and 9 months. Quantification is shown in panels B and C. =6. Scale bars: 50m. (D) 6 and 9 months immunostaining for VIM. Quantification is shown in panels E and F. =6. Scale bars: 50m. (G) Sixmonth kidneys: fluorescence detection of LTL binding (green) and SMA (red), and immunohistochemistry for SMA. Scale bars: 100m. (H) Ninemonth kidneys: fluorescence detection of LTL binding (green) and SMA (red) and immunostaining for SMA. Scale bars: 50m. (I, J) Protein levels of the epithelial cell marker Cdh1 in 6 and 9month kidneys ( =6). MeanSD from three to five experiments. **** <0.0001, *** <0.001, ** <0.01, * <0.05.",yes
PMC8476317,Figure_1,oa_package/42/1f/PMC8476317.tar.gz,"[' Now considered a subtype of endometrioid adenocarcinoma with mucinous differentiation ().', '.']",Fig. 1. Endometrioid adenocarcinoma with mucinous differentiation.,yes
PMC10471314,Figure_3,oa_package/f6/1b/PMC10471314.tar.gz,['Intraoperative picture of the patient s left eye.'],Figure 3 Intraoperative picture of the patients left eye. Intraoperative picture of the patients left eye during pars plana vitrectomy after clearance of the hemorrhage behind the intraocular lens in the posterior chamber. The retina was seen abutting the intraocular lens due to an extensive suprachoroidal hemorrhage anteriorly at the 2-4 and 6-10 oclock positions.,yes
PMC10691880,Figure_6,oa_package/bd/90/PMC10691880.tar.gz,"[', Budapest, Hungary) (Supplementary ).', '026) (A).', '038) (B).', '\nCorpus callosum thickness.']","Figure 5 ( ) Open field test at 24hours and 4 weeks post-impacts. Repeated measures revealed that locomotor activity (all zones) decreased significantly over time for RSHI animals (42.5%) and sham (52.6%), but not controls (27.8%) ( ). After stratification by sex ( , ), this effect remained significant for male RSHI, male sham and female sham, but not female RSHI. Similarly, centre zone locomotor activity decreased significantly over time for RSHI (75.5%) and sham (69.4%), but not controls (14.9%) ( ). After stratification by sex ( , ), this effect remained significant for male RSHI, male sham and female sham, but not female RSHI. Each data point in panels represents the value of an individual animal. ( ) Elevated plus maze assessment at 24hours and 4 weeks post-impacts. Repeated measures revealed that relative to controls, RSHI and sham animals spent significantly less time in the open arms at 4 weeks compared to 24hours post-impacts (65.5% and 81.3%, respectively) ( ). After stratification by sex, significant decrease in open arm exploration was observed in male RSHI ( ) but not female RSHI ( ). Each data point in panels represents the value of an individual animal. ( , ) Novel object placement test at 24hours and 4 weeks post-impacts. At 24hours, there were no group differences in displaced object versus non-displaced object exploration ( ). However, at 4 weeks, RSHI animals spent less time exploring the displaced object than control animals (one-way ANOVA with Tukeys multiple comparisons test) ( ). Each data point in panels and represents the value of an individual animal. < 0.10, * < 0.05, ** < 0.01, *** < 0.001. SEM, standard error of the mean.",yes
PMC10986346,Figure_2,oa_package/9c/17/PMC10986346.tar.gz,[],"Figura 2 Ecocardiograma transtorcico correspondiente al caso 6. Vista paraesternal eje largo que muestra engrosamiento del septum interventricular y pared posterior del ventrculo izquierdo. Vista apical 4-cmaras que muestra engrosamiento de las paredes de ambos ventrculos, del septum interventricular e interatrial, dilatacin biatrial, y engrosamiento de los velos de las vlvulas mitral y tricspide. Strain longitudinal global reducido (-11), con valores conservados en pex y reduccin a nivel basal y medio. Mapa polar que muestra todos los segmentos del ventrculo izquierdo con patrn caracterstico en ojo de buey.",yes
PMC6636370,Figure_2,oa_package/b3/02/PMC6636370.tar.gz,"[' Location: we recommend subdividing the carotids into distal\nand proximal common carotid, bifurcation, external branch, and proximal and\nmedial internal branch ().', '\nRight carotid and its anatomical subdivisions recommended by the\ngroup (adapted from the Mannheim study).']",Figure 2 Right carotid and its anatomical subdivisions recommended by thegroup (adapted from the Mannheim study).9 CC: common carotid; IB:internal branch; BCA: brachiocephalic artery.,yes
PMC9730375,Figure_4,oa_package/2b/82/PMC9730375.tar.gz,"['The intervention was guided by fluoroscopy and transoesophageal echocardiography (figure 4).', 'Stenting of left inferior pulmonary vein (LIPV) (A): severe stenosis of LIPV in fluoroscopy (yellow arrow on upper figure) and transoesophageal echocardiography (red arrows on lower figure): LIPV systolodiastolic flow mean gradient 4.']",Figure 4 Stenting of left inferior pulmonary vein (LIPV) (A): severe stenosis of LIPV in fluoroscopy (yellow arrow on upper figure) and transoesophageal echocardiography (red arrows on lower figure): LIPV systolodiastolic flow mean gradient 4.3mm Hg and maximum gradient 10mm Hg. (B) stenting (Omni elite 8.019mm) of LIPV in fluoroscopy (yellow arrow on upper figure) and transoesophageal echocardiography (red arrows on lower figure). (C) Final result in fluoroscopy (yellow arrows on upper figure) and transoesophageal echocardiography (red arrows on lower figure): left inferior pulmonary vein systolo-diastolic flow mean gradient 2mm Hg and maximum gradient 7mm Hg,yes
PMC10775915,Figure_2,oa_package/4e/a8/PMC10775915.tar.gz,"['Punch biopsy of the lesion on the patient s right arm, H E 200xThe epidermis is seen with full-thickness necrosis, orthokeratosis, and subepidermal cleft formation overlying the dermis with minimal perivascular chronic inflammation, consistent with the clinical impression of SJS.']","Figure 2 Punch biopsy of the lesion on the patients right arm, H&E 200x The epidermis is seen with full-thickness necrosis, orthokeratosis, and subepidermal cleft formation overlying the dermis with minimal perivascular chronic inflammation, consistent with the clinical impression of SJS. H&E: hematoxylin and eosin",yes
PMC8605355,Figure_4,oa_package/31/d4/PMC8605355.tar.gz,"['.', '.']","Fig. 4. A scatter plot showing the amount of DNA detected by ddPCR in MSRE-treated DNA from tumor (red, =48) and non-tumor (blue, =22) lung tissue. The same amount of tissue-derived DNA was used for both the tumor and tumor-free samples. The methylated DNA copy number was normalized to the total amount of input DNA. ( ) A scatter plot showing the amount of DNA detected by ddPCR in MSRE-treated cfDNA from healthy blood donor (blue, =39) early stage NSCLC (red, =48) and late stage NSCLC samples (green, =61). cfDNA was isolated from 1ml of plasma and the same volume of eluted DNA was used in the digestion/ddPCR reaction. The methylated DNA copy number is expressed per ml of plasma used for cfDNA isolation. ( ) Scatter plots showing the stratification of early stage patients into high and low methylated groups based on median methylation levels in tumor DNA (left panel) and the abundance of methylated cfDNA from the same patients from the high and low groups (right panel). ( ) A receiver operator characteristic curve showing the sensitivity and specificity performance of methylated cfDNA on late stage NSCLC patients. ( ) A receiver operator characteristic curve showing the sensitivity and specificity performance of methylated cfDNA on early stage NSCLC patients. The scatter plots show the mean methylated cfDNA copy number per ml of plasma and the standard deviation. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).",yes
PMC10015112,Figure_3,oa_package/bb/6f/PMC10015112.tar.gz,"['4,10,11 Taken together, IL-36 affects both the innate and adaptive immune system leading to an unrelenting, pro-inflammatory cycle ().', 'Interleukin 36 (IL-36) plays key role in the inflammatory cycle of pyoderma gangrenosum.']","Fig 3 Interleukin 36 (IL-36) plays key role in the inflammatory cycle of pyoderma gangrenosum. Upon skin injury, there is RNA release which activates the innate immune response and leads to neutrophil activation and degranulation. At the same time, keratinocyte injury leads to IL-36 release. Simultaneous occurrence of these events leads to activation of IL-36 by neutrophil-derived proteases. Activated IL-36 leads to increased expression of pro-inflammatory, neutrophil recruiting cytokines interleukin 8, TNF, CXCL-8, interleukin 17A, and interleukin 6. Further, IL-36 activation also leads to skewed Th1 differentiation with decreased Treg differentiation; thereby amplifying the inflammatory response and progression of disease. Spesolimab, an IL-36 receptor blocker, inhibits pro-inflammatory downstream effects of activated IL-36; thereby breaking the inflammatory cycle in pyoderma gangrenosum. , CXC-motif chemokine ligand-8; , interleukin 6; , interleukin 8; , interleukin 17A; , interleukin 36; , interferon- ; , interferon-; , T helper 1 cell; , tumor necrosis factor.",yes
PMC9914430,Figure_18,oa_package/cc/8f/PMC9914430.tar.gz,[],"Figure 18 GradCam analysis of four class photos at the final convolution layer in the Xception, VGG19, and Resnet50 models. First row or images ( ): shows superimposed images of different classes in the last layer of the Xception model. Images ( ) or Second row: shows superimposed images of different classes in the last layer of the VGG19 model. Images ( ) or Third row: shows superimposed images of different classes in the last layer of the Resnet model. The first, second, third, and fourth columns display superimposed images of normal. COVID, Lung opacity and non-COVID viral pneumonia classes. Xception and Resnet models classified all the images correctly, but VGG19 misclassified Normal and non-COVID viral pneumonia classes.",yes
PMC7033067,Figure_5,oa_package/98/90/PMC7033067.tar.gz,"['5.', 'Clinical examples illustrating the diffusion levels presented in ']",Fig. 5 Clinical examples illustrating the diffusion levels presented in Fig. and Table,yes
PMC5030675,Figure_5,oa_package/5f/ad/PMC5030675.tar.gz,"[', which corresponds to a dose below the 50% lethal dose of 75 parasites (A D) or a higher dose of 500 blood-trypomastigotes, respectively (', 'The parasitemia did not differ between C57BL/6 and IL-22 / mice during the course of low dose infection (A).', 'Accordingly, parasite burdens in spleens, livers and hearts were decreased at day 28 post infection in the absence of IL-22 (B).', 'IL-22 / mice showed a significantly increased body weight loss during early infection (C), which could be a sign for pathology.', 'However the increased body weight loss in infected IL-22 / mice was only transient and did not influence the mortality rate (D).', 'cruzi infection (E), however they also exhibited an increase in body weight loss (', 'The analysis of tissue burdens during early infection with 500 parasites revealed an unaltered parasite burden in the spleens and the small intestines and increased burdens in the livers of IL-22 / mice (G).', 'Microscopic analysis of hematoxylin/eosin (H E)-stained heart sections revealed that detectable parasite nests were very rare (only present in one of five mice in both groups) and similar in both mouse groups (H).', 'cruzi parasites were also found in intestinal structures (G), we also analyzed H E-stained sections of the colon (', 'cruzi parasites were present in intestinal structures as shown by real-time PCR (G), these structures did not show any signs of pathology in both mouse groups (', 'org/1999/xlink"" xlink:href=""srep32927-f4""/>Undiminished control of T.']","Figure 5 Undiminished control of infection. In ( ) C57BL/6 and IL-22 mice were infected i.p. with a sublethal dose of 50 blood trypomastigotes. Parasitemia ( ), tissue parasite burdens ( ) body weight changes ( ) and survival ( ) were assessed during the acute infection. Results are expressed as meansstandard deviations of 10 mice per group. One out of three independent experiments with comparable results is shown. In ( ) C57BL/6 and IL-22 mice were infected i.p. with a high dose of 500 blood trypomastigotes. Parasitemia ( ), body weight changes ( ) and tissue parasite burdens ( ) were assessed during the acute infection. Results are expressed as meansstandard deviations of 5 mice per group (2 uninfected mice per group). ( ) H&E-stained heart sections were analyzed for the presence of parasite nests (arrows). 5 mice per group were analyzed. Magnification 400x. * and ** indicate statistical significance (p0.05 and p0.01, respectively) compared to C57BL/6 wild-type mice.",yes
PMC10866854,Figure_15,oa_package/36/fb/PMC10866854.tar.gz,[],"Fig. 15 Difference between a type I curve and a type II curve. Same case as illustrated previously: Axial T2-weighted sequence without fat saturation. DCE MRI T1-weighted sequence with corresponding curve of the tissular component showing a type I curve corresponding to a cystadenofibroma. Other case with ( ) Axial T2-weighted sequence without fat saturation. , DCE MRI T1-weighted sequence with corresponding curve of the tissular component showing a type II curve corresponding to a borderline serous cystadenoma",yes
PMC8601609,Figure_3,oa_package/10/6b/PMC8601609.tar.gz,"['Whereas both antioxidants were able to reduce skin neutrophil infiltration (C and 3D), NFKB activity (E and 3F) and morphological alterations (S2 FigRelated to .', ' Quantification of keratinocyte aggregation foci in the tail of lyz:dsRED larvae shown in (A, C, E, G, I) and detailed representative merge images (brightfield and red channel) (B, D, F, H, J) upon their treatment with with vehicle (DMSO), 100 M NAC (A, B), 100 M mito-TEMPO (MT), and 100 nM tempol (T) (C, D), 250 M apocynin (E, F), or upon genetic inhibition of nox1 and nox5 (G, H), and nox4 (I, J).', 's003"" position=""float"" content-type=""local-data"">S3 FigRelated to .', 'For example, C/D, 3E/F, 3G/H, 3L/M, S2C/D, S2G/H, ', 'Authors also did not address the concerns that the representative images were not consistent with the quantification data shown in C/D, 3E/F, 3H/I, 3L/M, S2C/D, S2G/H, ', 'I: protein expression of nox1, nox4 and nox 5 should be checked after genetic inhibition using CRISPR/Cas9 technique.', '2A, C, E, G, I, K, L; A, C, J, E, G, L; ']",10.1371/journal.pbio.3001455.g003,yes
PMC8395232,Figure_4,oa_package/06/0d/PMC8395232.tar.gz,"['In addition, activation of type I IFN signaling by mt-derived nucleic acids may lead to the propagation of an inflammatory response, which would further contribute to secondary damage and neurodegeneration (, Table 1).', 'Mitochondrial nucleic acids-driven inflammation as a critical regulator of disease progression in neurodegenerative processes.']","Figure 4 Mitochondrial nucleic acids-driven inflammation as a critical regulator of disease progression in neurodegenerative processes. Dysregulation of mitochondrial function leads to the activation of type I IFN signaling by mt-derived nucleic acids. The activation and the propagation of the inflammatory response may contribute to secondary damage and neurodegeneration. Besides mitochondrial dysfunction promoting inflammation, inflammation per se can lead to mitochondrial dysfunction, suggesting the existence of a pro-inflammatory loop with mitochondria as a central player.",yes
PMC9581928,Figure_3,oa_package/2b/d7/PMC9581928.tar.gz,"[' 3c, Supplementary Data 2.', ' 3a).', ' 3b, n = 3 per variable).', ' 3c at 15 60 min).', ' 3d1-2, d1 2 ), transmission (TEM, ', ' 3d3, 3 , 5, 5 , 6 , e1-5), and correlative-light (CLEM, ', ' 3d4-5, d4 , e6 9) electron microscopy provided further insight: in controls, PRs were positioned with their soma and nucleus in the ONL below the OLM and their PIS and POS above (Figs.', '3d5 6 ).', '3d3 , 4 ; e1-9) with their nuclei delaminating and passing through the OLM, indicated by hourglass-shaped PR nuclei (validated with RCVRN by CLEM; ', '3e6, e7).', '3e5).', 'HBEGF-TNF treatment induces photoreceptor cell extrusion and photoreceptor inner segment (PIS) defects.', 'HBEGF-TNF induces gliosis, retinal dyslamination, and scar formationBased on our hypothesis, HT might not only induce PR but also MG pathologies (', ' 3b) of whole living HROs, the optical plane was positioned at the PIS level and imaged from above at an orthogonal angle (en-face).', ' 3c and Supplementary Movies 2 4).']","Fig. 3 HBEGF-TNF treatment induces photoreceptor cell extrusion and photoreceptor inner segment (PIS) defects. Live-imaging of HROs. Differential interference contrast. Spinning disk confocal microscopy. , HT-HROs and controls (CTRL) live in culture (Supplementary Data and Movies ). SiR-DNA cell nuclei live-dye labeling (acutely applied for imaging). En-face imaging: ectopic nuclei appeared after 5 days in HT-HRO on its surface, but not in controls (optical plane positioned at the PIS level). Independent experiments: =10 HROs (Supplementary Data ). Cross-sectional image series from Supplementary Movie : cell nuclei move out of the apical organoid boundary onto its surface (yellow line). Cell nuclei in the outer retina appear round in controls and before extrusion, and transiently became hourglass-like shaped during extrusion. Independent experiments of =8 HROs (Supplementary Data ). HT-HROs compared to CTRL analyzed by scanning (SEM) electron microscopy (EM) of (12, 12), transmission EM (TEM) of epoxy-embedded HROs (3, 5, 3, 56), and correlative-light electron microscopy (CLEM) of ultrathin cryosections (4, 4): RCVRN (photoreceptors, P), SLC1A3 (Mller glia, MG), and DAPI (extruded (*) cell nuclei) staining, visualized by immunofluorescence and gold particles (black dots, 10nm gold). =1 independent experiment ( ) with =3 (TEM) and =5 (SEM) HROs ( ) analyzed. TEM image series: photoreceptors (P, green pseudocolor) during or after extrusion (10-days HT). Cell junctions (OLM): red labeled. Photoreceptor nuclei: asterisks. Double asterisk: a dead nucleus ( ). CLEM of HT-HROs ( , : 10-days HT; , : 20 days HT) immunolabeled for RCVRN ( ) or ARR3 ( ) and DAPI confirms that photoreceptors become extruded. Extruded cells (asterisks) and a photoreceptor in the process of being squeezed through the OLM, indicated by hourglass-shaped nucleus and position of cell junctions (white dashed line ( ), red pseudocolor ( )), are visible. Boxed area: higher magnification in ( ). PIS (green) of a cone (ARR3 labeled) in HT-HRO. TEM and CLEM: each =1, =3 HROs/N. Mitochondria (M). Outer (ONL)/inner (INL) nuclear layer. Scale bars: 25m, , , 10m, 50m, ; ROI of ; 5m, , ; 2m, ROI of 500nm, 200nm.",yes
PMC6927526,Figure_11,oa_package/e9/a0/PMC6927526.tar.gz,[],"Figure 4. Fas-FasL interaction between effector CD4 T cells and BMDCs leads to caspase-8 dependent cleavage of pro-IL-1 (a) lL-1, as quantified by ELISA, or (b) detected by immunoblot analysis of cleaved IL-1 (p17) in the supernatants of WT T 0 cells co-cultured with WT or BMDCs in the presence of CD3 Ab and neutralizing FasL Ab (10g/mL) for 18 h. (a) Error bars indicate SEM for n=3 independent experiments. (b) Data are representative of two independent experiments. (c) Immunoblot analysis of cleaved casp-8 (p18) in the lysates of WT BMDCs cultured with WT T 0 cells in the presence of CD3 Ab and neutralizing FasL Ab (10g/mL) or TNF Ab (20g/mL) for 12 h. Data are representative of two independent experiments. (d) Immunoblot analysis of pro-IL-1 (p35) or cleaved IL-1 (p17) in the cell lysates or the supernatants, respectively, of WT BMDCs co-cultured with WT T 0 cells in the presence of CD3 Ab and IETD (10M) for 18 h. Data are representative of two independent experiments. (e) lL-1 was quantified by ELISA in the supernatants of WT T 0 cells co-cultured with WT, , or BMDCs in the presence of CD3 Ab for 18 h. Error bars indicate SEM for n=3 independent experiments. Statistical analysis was performed by paired, one-tailed Students -test. <0.05.",yes
PMC11082483,Figure_2,oa_package/e7/3d/PMC11082483.tar.gz,"['1177/20480040241248924"" ext-link-type=""uri"">Video 1; ) showed severe ostial stenosis of the left anterior descending artery (LAD) and in its middle segment at the bifurcation with the 3rd diagonal (DG) (Medina 1-1-1), subocclusive ostial stenosis of the 2nd DG, mild stenosis in the proximal segment and moderate stenosis in the distal segment of the circumflex artery (Cx) (after the birth of the 1st marginal; Medina 0-1-0) and an anomalous birth of right coronary artery that did not present angiographically significant lesions.', '.', '1177_20480040241248924-fig2"" position=""float""/>Cx: circumflex artery; DG: diagonal artery; LAD: left anterior descending artery; RCA: right coronary artery.']","Figure 2. (a) Moderate stenosis in distal segment of Cx (white thin arrow), after the 1st marginal artery's birth. (b, d) Subocclusive ostial stenosis of the 2nd DG and severe stenosis in mid-segment of LAD at the bifurcation with 3rd DG (Medina 1-1-1) (blue thick arrow). (c) Severe ostial stenosis of LAD (blue arrowhead). (e, f) Anomalous birth of RCA (blue asterisk) without angiographically significant lesions.",yes
PMC11598915,Figure_6,oa_package/a4/6f/PMC11598915.tar.gz,"['The nucleated cells identified were usually a mix of non-degenerate neutrophils, monocytes/macrophages, and small lymphocytes ().', 'Cytology of a feline infectious peritonitis (FIP) effusion.']","Figure 6 Cytology of a feline infectious peritonitis (FIP) effusion. Mixed cell population (non-degenerate neutrophils and macrophages) dispersed in a granular eosinophilic proteinaceous background commonly observed in FIP effusions. May-Grnwald Giemsa, 400.",yes
PMC4572472,Figure_1,oa_package/0e/b4/PMC4572472.tar.gz,"['Cardiac MRI was done which showed diffuse edema on T2 weighted image (), hyperemia on T1 weighted after gadolinium ((a)), and fibrosis on transmural late gadolinium enhancement ((b)) of the wall of the right ventricle, consistent with isolated RV myocarditis.', '0-00226465742420912T2 weighted image showing diffuse RV edema.']",Figure 1 T2 weighted image showing diffuse RV edema.,yes
PMC7587296,Figure_15,oa_package/a3/c2/PMC7587296.tar.gz,[],Figure 7b. Transition from progressive stage to peak stage in a 69-year-old man withCOVID-19. Axial nonenhanced chest CT image (lungwindow) obtained at hospital admission shows bilateral areas ofground-glass opacities and crazy-paving appearance. Axial contrast-enhanced chest CT image (lung window) obtained after 7days shows progression from ground-glass opacities to multifocalorganizing consolidation.,yes
PMC4462811,Figure_4,oa_package/a1/22/PMC4462811.tar.gz,"['[23459101213163060] CT scan, however, may reveal one or more cystic dilatations in the pancreas (branch type) or diffuse or segmental dilatation of the MPD (main duct type) with or without polypoidal lesion in patients with IPMN [].', 'Multiple cystic dilations of the side branches with the largest lesion in the head (curved arrow) and smaller lesions in the body and the tail (straight arrows)CT scan showing a large cystic lesion in the head of the pancreas in a patient with MD-IPMNEUS adds a further dimension in the diagnosis of IPMN by improving the accuracy of the assessment of pancreatic parenchyma.']",Figure 4 CT scan showing a large cystic lesion in the head of the pancreas in a patient with MD-IPMN,yes
PMC10658974,Figure_1,oa_package/eb/d2/PMC10658974.tar.gz,"[' 1).', 'Retrocardiac multicystic mass on CECTOn MRI (']",Fig. 1 Retrocardiac multicystic mass on CECT,yes
PMC6606791,Figure_1,oa_package/03/70/PMC6606791.tar.gz,"['Polydatin structure is illustrated in \nA\n.', '01, \nB\n).', 'TTC staining entirely demarcated the infarcted area from the deep red area of intact tissue (\nC\n), with sham-operated rats showed no infarction.', '01, \nC\n).', 'Effect of polydatin on neurological scores, brain infarction and neurodegeneration.', 'A substantial difference was observed in the neuropil of ischemic cortex as compared with sham-operated control, where ischemia caused robust neuronal cell loss in the cortex and polydatin treatment attenuated this damage (\nD\n, p 0.', 'Moreover, aberrant morphological neuronal features were identified in the MCAO cortex, including alteration in neuronal size and shape (swelling and a scalloped, angular nature), alteration in color staining (cytoplasmic eosinophilia/pyknosis, nuclear basophilia), and vacuolation (\nD\n).']","Figure 1 Effect of polydatin on neurological scores, brain infarction and neurodegeneration. Structure of polydatin. Polydatin attenuated neurological deficits. Neurological test was conducted 24 h after ischemia. Poly+MCAO rats had significantly improvement of neurological deficits ( < 0.01) compared to MCAO group. Brain coronal sections were stained with TTC, which distinguishes between ischemic and non-ischemic areas (n = 510/group). Representative photomicrograph of cresyl violet staining showing the extent of surviving neurons in the cortex, scale bar = 50 m. (a, b) Cytoplasmic swelling and scalloped neurons with intense cytoplasmic eosinophilia and nuclear basophilia. These changes result from neuronal ischemia, which impairs metabolism in the tissue. (c) Cytoplasmic fading of neurons, which invariably occurs in neurons at later stages of necrosis (ghost neurons). (d) Some cells had shrunken appearance along with pyknotic nuclei. Intensive neuropil vacuolation can be seen in ischemic cortex (MCAO). *** < 0.001; **, < 0.01; < 0.05.",yes
PMC4330237,Figure_10,oa_package/b9/9b/PMC4330237.tar.gz,[],Fig. 10 Virtual implant treatment planning with CBCT data: software can help implant treatment planning through simulation and 3D reformation. (Courtesy Jari Mauno.),yes
PMC5517505,Figure_4,oa_package/f7/7f/PMC5517505.tar.gz,"[' 4A D).', 'Validation of key regulatory genes involved in oxygen glucose deprivation (OGD) pathophysiology.', '\nValidation of expression changes of selected key genes in an in vivo ischemic stroke modelAlthough powerful to examine the cell autonomous effects, a key issue for in vitro assays is how the results correlate with the in vivo scenarios.', ' 4] that were up-regulated and down-regulated after OGD, respectively.', ' 4B,C, and Supplementary Tables).']","Figure 4 Validation of key regulatory genes involved in oxygen glucose deprivation (OGD) pathophysiology. ) The selected differentially expressed genes (DEGs) for OGD 45min/R 0h vs OGD 0min/R 0h ( ); OGD 45min/R 6h vs OGD 45min/R 0h ( ); OGD 45min/R 12h vs OGD 45min/R 0h ( ); and OGD 45min/R 18h vs OGD 45min/R 0h ( ) were validated by qPCR. For each time point, neurons were collected and pooled from hippocampal cells isolated from six embryos. Triplicates (from pooled RNAs) were then performed.",yes
PMC8128770,Figure_4,oa_package/ca/d2/PMC8128770.tar.gz,[],"FIGURE 4 Posterior wall of abdomen proper. Crosssection of the lumbar region of abdomen illustrating the posterior wall of abdomen proper (PWAP) consisting of the quadratus lumborum [QL], psoas muscles (only the psoas major [PS] shown in this cross section) and the body and transverse process of the lumbar vertebra. The posterior boundary of the PWAP is constituted by the middle layer of the thoracolumbar fascia and the lateral raphe, separating it from the lumbar region of back and its constitutional parts including the erector spinae. The midaxillary line separates the PWAP from the anterior and lateral abdominal wall. The cross section is from the Visible Human Project (slice afv1588c, courtesy of the U.S. National Library of Medicine)",yes
PMC3083399,Figure_3,oa_package/89/bb/PMC3083399.tar.gz,"['g003Complex I expression in the murine hippocampus.', 'Complex I gene expression in the hippocampusIn the hippocampus we found a highly specific expression pattern during development and analyzed four hippocampal subfields: the CA1, distal and proximal CA3 regions and the dentate gyrus ().', '001; ).']",10.1371/journal.pone.0018897.g003,yes
PMC4190686,Figure_3,oa_package/31/7f/PMC4190686.tar.gz,"['Stimulation and culture of IL-17+ T cells in the presence of IL-2 down-regulated IL-17 and promoted plasticity toward an intermediate Th1/Th17 (IL-17+IFN +) and Th1 (IL-17 IFN +) phenotype (A).', 'IL-23, although important for murine Th17 plasticity, did not promote a Th1 phenotype (C).', 'Most importantly, the IFN +GM-CSF+ T cell population identified in the joint was rapidly induced following culture of IL-17+ cells in the presence of IL-12 (E).']","Figure 3 Transition of Th17 cells toward a GM-CSFexpressing phenotype in response to IL-12. A and B, IL-17+ and IL-17 CD4+ T cells from healthy control PBMCs were sorted by flow cytometry following cytokine capture and analyzed for expression of IFN and IL-17 (A) or IFN and GM-CSF (B) at baseline and after culture for 4 days in the presence of IL-2 (alone or with IL-12) prior to restimulation with phorbol myristate acetate and ionomycin in the presence of brefeldin A and analysis for cytokine expression. Results of a representative experiment (of 5 performed) are shown. C, IL-17+ CD4+ T cells (gray bars) and IL-17 CD4+ T cells (black bars) from healthy control PBMCs were cultured as described in A (as well as with IL-2 plus IL-23), and the proportion of live cells expressing IFN but not IL-17 was determined. D and E, IL-17+ CD4+ T cells (gray bars) and IL-17 CD4+ T cells (black bars) from healthy control PBMCs were cultured as described in B (as well as with IL-2 plus IL-23), and the proportion of live cells expressing GM-CSF (D) or IFN and GM-CSF (E) was determined. Values in CE are the mean SEM. = < 0.05; = < 0.01, by two-way ANOVA with Bonferroni correction for multiple comparisons. See for definitions.",yes
PMC10514485,Figure_6,oa_package/4e/a4/PMC10514485.tar.gz,[],"Figure6 NKG2D mediates acute and chronic neonatal LPS-induced T cell activation and IL-17a production. Nkg2d mRNA expression was increased in neonatal lung after acute and chronic exposure to LPS. LPS induced neonatal lung mRNA expression of the NKG2D ligands Rae1 and Mult1, as well as the master regulator of DNA damage p53. We inoculated immature C57BL/6J mice with LPS or PBS intranasally on DOL 3, 5, 7 and 10. One hour prior to each PBS or LPS treatment, mice were injected with IgG or anti-NKG2D antibody (Clone HMG2D) intraperitoneally. Lungs were examined by flow cytometry after acute and chronic LPS exposure. The NKG2D receptor expression, as a marker of activation, was detected on T cells using a different clone of the anti-mouse NKG2D antibody (clone CX5). NKG2D+ T cells were increased after acute and chronic LPS treatment in the neonatal lung, consistent with T cell activation. IL-17a+NKG2D+ T cells were also increased. Anti-NKG2D antibody treatment attenuated the effects of LPS on the activated NKG2D+ T cells and the fraction of these cells that produced IL-17a. This analysis quantified native IL-17a expression only using a Protein Transportation Inhibitors cocktail. Per cell IL-17a expression (MFI) in NKG2D+ T cells after LPS exposure was quantified and the effect of anti-NKG2D treatment was assessed. Both acute and chronic LPS exposure induced whole lung mRNA expression and this effect was inhibited with anti-NKG2D treatment. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, ns non-significant (one-way ANOVA and unpaired test). Each open circle indicates one baby mouse. One of at least two independent experiments is presented.",yes
PMC3465553,Figure_2,oa_package/6d/12/PMC3465553.tar.gz,"['6 of infection, when approximately 50% of total infiltrating lymphocytes were NK cells (A, left panel).', 'Both in influenza- and in PVM-infected mice, BAL NK cells displayed an activated phenotype (high CD69) and produced IFN upon stimulation ex vivo (B and C), indicating that they were functional.', 'Still, like HKx31, infection with PR8 (150 EID50) induced a prominent early NK cell influx into the airways (D, d.', '(D).', 'ppt""/>Supplementary Pre-existing PVM-specific CD8+ T cells diminish severe cellular infiltration upon intranasal PVM infection.', 'NK cell responses in PVM-infected mice compared to influenza-infected mice.', 'determined by tetramer staining as described in the legend to .']","Fig. 2 NK cell responses in PVM-infected mice compared to influenza-infected mice. BALB/c mice were infected i.n. with approximately 25pfu PVM or 110 EID influenza A/HK-x31 and sacrificed at the indicated days p.i. (A) NK cells (TCR DX5 ) as percentage of total lymphocytes (left panel) or in absolute numbers (right panel) in the BAL, as determined by flow cytometry. (B) Mean fluorescence intensities (MFI) of CD69 expression on NK cells in the BAL. (C) Percentage of IFN producing NK cells in the BAL after restimulation with YAC cells (1:1) in the presence of monensin for 4h. (D) Mice were infected with 150 EID influenza PR8, 25pfu PVM (normal dose) or 1250pfu PVM (high dose) and absolute numbers of NK cells (DX5 NKp46 TCR ) in the BAL were determined. Results are shown as meanSEM for 3 mice per group. n.d., not determined.",yes
PMC6927550,Figure_4,oa_package/c5/78/PMC6927550.tar.gz,"['Immunohistochemical analysis of mucins and trefoil factors in the gastric corpus of WT and Muc5ac / mice and its correlation with Hp infectionAs shown in A, strong Muc5ac immunopositivity was observed primarily in the surface and foveolar epithelial pits of the gastric corpus of uninfected WT stomach, with infrequent staining of the glandular neck region.', 'The corpus of Muc5ac / mice, as expected, did not show any specific Muc5ac immunostaining, although mild non-specific background was observed within the epithelial stroma (B).', 'Muc5ac immunopositivity was seen in the apical foveolar pits of uninfected WT stomach and there was also a reduction in Muc5ac staining within foveolar pits of the gastric corpus overlying hyperplastic and mucous metaplastic regions of Hp-infected WT mice (C, D).', 'Muc1 was highly expressed in foveolar pits and also moderately within deeper glands of the gastric corpus in both uninfected WT and Muc5ac / mice (E).', 'In Hp-infected WT and Muc5ac / mice, Muc1 immunostaining was seen prominently within most hyperplastic foveolar and glandular regions (E,G) but was frequently reduced in areas of mucous metaplasia especially in the corpus of WT Hp-infected stomachs (H).', ':Immunohistochemistry of Muc5ac and Muc1 in the gastric corpusMuc5ac IHC: A-D, Prominent Muc5ac expression in the gastric foveolar region of the WT uninfected control (A) and lack of expression in uninfected Muc5ac / mice at 32 wpi (B).']","Figure 4: Immunohistochemistry of Muc5ac and Muc1 in the gastric corpus Muc5ac IHC: A-D, Prominent Muc5ac expression in the gastric foveolar region of the WT uninfected control (A) and lack of expression in uninfected mice at 32 wpi (B). Muc5ac expression in WT -infected stomach in areas of pseudopyloric metaplasia or mild mucous metaplasia (C, MM mild) compared to a reduction in surface foveolar expression in areas of severe glandular mucous metaplasia (D, MM severe 32 wpi. Bars: 160M, All images Muc1 IHC: E-F: Muc1 expression is robust in the upper 1/3 and lower 1/3 of normal corpus (E, WT uninfected, Bar:160M) and is enhanced in hyperplastic glands of infected WT and Muc gastric corpus (F, G, Bar: 400M). A slight reduction in intensity of Muc1 staining in areas of glandular mucous metaplasia (arrows) as shown in the higher magnification of inset (H, Bar: 80M)",yes
PMC11376318,Figure_3,oa_package/03/ef/PMC11376318.tar.gz,[' Positron emission tomography (PET) scan demonstrating no evidence of metastasisThe final pathology demonstrated pT2a spitzoid melanoma without ulceration or mitoses with scattered spitzoid cells in the sentinel lymph node biopsy of the left inguinal lymph node.'],Figure 3 Positron emission tomography (PET) scan demonstrating no evidence of metastasis,yes
PMC8432422,Figure_2,oa_package/b4/32/PMC8432422.tar.gz,['CT chest demonstrated a new 3.'],"Figure 2 CT chest demonstrated a new 3.9 cm long segment of dissection of the ascending thoracic aorta, originating 3 cm distal to the aortic root. Arrow in figure pointing to dissection.",yes
PMC6261538,Figure_8,oa_package/b6/eb/PMC6261538.tar.gz,"['037) (A), while %BV/TV was maintained (C).', '048) at both time points (E).', 'Interestingly, the CD14-deficient mice maintained baseline levels of BMD (B), % BV/TV (D), as well as Tb.', '(F) after DMM surgery at both 6 and 19 weeks.', 'g008Micro-CT analysis of SCB in na ve and operated (sham or DMM) joints at 6 and 19 weeks post-surgery.', 'CD14 deficiency completely protected mice from post-DMM SCB changes ().']",10.1371/journal.pone.0206217.g008,yes
PMC11444080,Figure_6,oa_package/37/9f/PMC11444080.tar.gz,"['Complete blot images are available in Supplementary .', 'Uncropped blots are included in Supplementary .', 'Uncropped blots are included in Supplementary .', '0555), in the cortex of human patients with epilepsy relative to age-matched control brains (A and B).', '0043) (C and D) and Y18 levels (P = 0.', '0292) in the epileptic brain (E and F).', 'A positive control from an Alzheimer s disease case confirmed the specificity of phospho-tau staining in the human epileptic brain (C F).', '0003) in epileptic human brain relative to controls (G and H).', '\nIncreased pTau (AT8, Y18), pFyn/SFK (Y416) and Fyn-tau and NR2B-PSD95 interactions in human epileptic brain.', 'Uncropped blots are included in Supplementary .', '0167) interactions revealed a significant upregulation compared to controls (I and J).', 'In addition, Co-IP of cortical lysates confirmed increased binding of Fyn to tau, compared to age-matched control, further corroborating our PLA results (K).', '79) (L).', '83) (L).', 'Uncropped blots are included in Supplementary .', 'Furthermore, a representative Fyn-tau Co-IP also confirms the occurrence of such interactions in temporal cortex of an epileptic patient (K).']","Figure 5 ( ) Representative images of PLA for Fyn-tau interactions in DG (white dots like in ) and NR2B-PSD95 interactions in CA3 (cyan dots like in ) co-labelled with a dendritic marker MAP2 (green, in both and ) show a significant increase of Fyn-tau/NR2B-PSD95 puncta/complexes ( ) in KA-treated rats at 3 months post-SE. Repeated measures two-way ANOVA with Sidaks . ( ) Spearman correlation showing positive correlations between hippocampal Fyn-tau/NR2B-PSD95 complexes with the numbers of convulsive SRS [average (Av.) seizure/min] and epileptiform spike rate (Av. spike/min), but not with the duration of seizures (Av. duration of seizures). ( ) Heatmap showing summary of Spearman correlation matrix between Fyn-tau associated proteins, interactions and EEG parameters. Black crosses correspond to non-significant correlations. Scale bar 100m. Bar graphs displayed all data points and expressed as mean SEM. Dots represent individual animals. The red-dotted curvilinear in the correlational plot represents a 95% CI for two means.",yes
PMC6433456,Figure_7,oa_package/ec/da/PMC6433456.tar.gz,[' Picture after three months of treatment with 4.'],"Figure 7 Picture after three months of treatment with 4.5 mg LDN orally In this picture, we can see complete remission of the disease, as pointed to by the arrow after three months of low-dose naltrexone (LDN). Symptom-free (Psoriasis Area Severity Index (PASI) score= 0). The patient was compliant with treatment and did not refer to any side effects of LDN during her treatment. The patient was very pleased with her results.",yes
PMC9805844,Figure_1,oa_package/c9/de/PMC9805844.tar.gz,"['By introducing radiopaque bougies, the diastasis between the proximal and distal pouches of the esophagus was measured ().', '0244_figure1"" position=""float""/>Table 1.']",FIG. 1. Measurement between esophageal ends.,yes
PMC11399754,Figure_3,oa_package/1d/13/PMC11399754.tar.gz,['.'],"Figure 3. Chest radiograph PA view, day 1.",yes
PMC3367920,Figure_3,oa_package/2b/db/PMC3367920.tar.gz,"['Normal fish appeared without any inflammation of the epicardium (a) and infected fish had focal to diffuse lymphocytic infiltration in the epicardium.', 'Inflammatory scores were similar at 7 wpc (b), lower by 8 wpc while at 9 and 10 wpc the inflammatory index increased with mean scores of 1.', 'The main difference between early (6 7 weeks) and late time points (9 10) was that the inflammatory changes extended from the epicardium and into the compact layer of the ventricle, and spreading along small vascular structures (d).', 'g003Histopathological changes in heart.']",10.1371/journal.pone.0037269.g003,yes
PMC9323105,Figure_3,oa_package/1a/3c/PMC9323105.tar.gz,"['Using human DMD muscle samples, we observed even larger reductions of miR-1 (95%), miR-133a (85%), and miR-133b (83%) in dystrophic skeletal muscles (A).', 'In the 6-month-old mdx5cv mouse TA, the mean fold-reduction of myomiRs ranged from 71% to 84% at 6 months old (B).', 'miR-1, miR-133a, and miR-133b are repressed in DMD and mdx5cv (MDX) skeletal muscles.']","Figure 3 miR-1, miR-133a, and miR-133b are repressed in DMD and (MDX) skeletal muscles. ( ) DMD human skeletal muscles show reduced miRNA expression levels by qPCR. ( ) 6-month-old (MDX) mouse tibialis anterior muscles show reduced miR-1, miR-133a, and miR-133b compared with wildtype (WT). Data points show biological replicates for each group. Bars show mean SEM. values: * < 0.05, ** < 0.01, and *** < 0.001. RQ, relative quantity.",yes
PMC11744626,Figure_6,oa_package/62/02/PMC11744626.tar.gz,"['Although grade 4 changes were predominant, grade 6 rats with the most severe cartilage degeneration were observed only in the MME model (\n\n).', '.', '1177_19476035231205680-fig6"" position=""float""/>The OA grade in OARSI score of both MMT and MME was significantly higher than that of the sham (Sham; 1.']","Figure 6. Histological images of the rat knee joint. Histological cartilage degeneration was evaluated by hematoxylin-eosin (HE) staining and Safranin-O staining. ( ) The sham group exhibited grade 1 to 2 changes in the medial articular surface of the tibia, characterized by superficial fibrillation and partial reduction of the matrix. ( ) In the MMT group, significant cartilage degeneration was observed, with the presence of complex cracks and large cavities indicating grade 3 to 4 degeneration and intercellular detachment. ( ) The MME group displayed the most severe degeneration, with extensive loss of cartilage matrix and its replacement by fibrous cartilage tissue. In some rats, there was almost complete loss of cartilage, and the transition to bone tissue appeared irregular. Notably, the MME model had grade 4 changes predominantly, and only in this group, grade 6 rats with the most severe cartilage degeneration were observed. Bar scale is 50 m. MMT = medial meniscus transection; MME = medial meniscus extrusion.",yes
PMC10979756,Figure_11,oa_package/bf/8b/PMC10979756.tar.gz,[],"Box plot 2 Meanstandard deviation of ramus mandibular height (Rm.H)/mandible length (Md.L), mandibular toothrow length (Mdt.L)/Md.L, orbital diameter (Or.D)/condylobasal length (Cb.L), Mt.L/Cb.L, braincase width (Bc.W)/Cb.L, muzzle width (Mu.W)/Cb.L and zygomatic width (Zg.W)/Cb.L ratios in Saanen goats.",yes
PMC6753509,Figure_4,oa_package/a8/09/PMC6753509.tar.gz,"['When a translocation\noccurs, the 2 separate color probes fuse and a third color signal (yellow) is present due\nto the overlapping of the other 2 colors ().', '1177_2374289519875647-fig4""/>How Does the Fusion of These 2 Genes Result in Leukemia?']","Figure 4. Fluorescence in situ hybridization (FISH) for fusion. Whena translocation occurs, the gene on chromosome 15 (red) and the gene on chromosome 17 (green) fuse together to produce a thirdcolor signal (yellow). PML gene indicates ( ) gene; RARA, retinoic acid receptor alpha.",yes
PMC8349650,Figure_7,oa_package/36/8d/PMC8349650.tar.gz,[],"FIGURE 7 The transcripts of inflammationrelated molecules (A), neurotrophic factors and phagocytosisrelated molecules in mice with repopulated microglia at 7 days after MPTP administration. (A) The transcripts of (a), (b), (c), (d), (e), (f), (g), (h), (i), (j) (k) and (l) in the striatum. (B) The transcripts of (a), (b) (c), (d), (e), (f), and (g) in the striatum. (C) The transcripts of (a) and (b) in the striatum. served as the reference gene. NS group: CDNSCD; MPTP group: CDMPTPCD; repopulationMPTP group: PLX3397/21dCD/7dMPTPCD. NS: normal saline; CD: control diet; PLX3397: PLX3397formulated diet. Oneway ANOVA followed by HolmSidaks or Dunns (as in Ab) multiple comparisons test was used for statistical analysis. * <.05, ** <.01, *** <.001. n=4",yes
PMC7690852,Figure_2,oa_package/0c/c2/PMC7690852.tar.gz,['miRNAs regulate different signaling pathways in osteoclastogenesis.'],Figure 2 miRNAs regulate different signaling pathways in osteoclastogenesis.,yes
PMC9672095,Figure_7,oa_package/f6/7d/PMC9672095.tar.gz,"[' 7A,C).', ' 7B,D).', 'Effects of SIRT3 activation by NR in cultured mouse microglial cell line: BV2 and Sirt3-silenced shSirt3BV2 cells were cultured in the absence and presence of increasing concentrations of nicotinamide riboside (NR) for 24 h (A,C) and 48 h (B,D).', 'DiscussionSirt3-/- mouse is a well-studied model to investigate the effects of MetS8,11,20,21.']","Figure 7 Effects of SIRT3activationby NR in cultured mouse microglial cell line: BV2 and Sirt3-silenced shSirt3BV2 cells were cultured in the absence and presence of increasing concentrations of nicotinamide riboside (NR) for 24h ( , ) and 48h ( , ). The cell lysates were prepared and immunoblotted for SIRT3 and IDE ( , ). Representative cropped images are presented for clarity and conciseness while the composite color images of full blots are presented in . The blots were quantitated by scanning and corrected for the levels of actin ( , ). MeanSE of results from 3 independent experiments are presented. <0.05; *, <0.01; **, <0.001 versus untreated control in respective cell lines, compared to Control untreated mice.",yes
PMC10448547,Figure_1,oa_package/fa/19/PMC10448547.tar.gz,"['At presentation the patient had multiple fractures in the wrist, femur, and tibia without any obvious trauma ().', 'Furthermore, the patient did not have any long bone or chest deformities and there was no vertebral compression on his lumbar X-ray (B).', '202240163138A) X-ray radiography of the patient s tibia and fibula, B) X-ray radiography of the patient s lumbar spine and kneesA) LRP5 gene structure and the mutation.']","Figure 1 A) X-ray radiography of the patients tibia and fibula, B) X-ray radiography of the patients lumbar spine and knees",yes
PMC4105768,Figure_2,oa_package/8c/bc/PMC4105768.tar.gz,"['.', 'Gluteal tendinosis or avulsion may present as lateral greater trochanteric pain (), and iliopsoas tendinosis due to impingement can lead to groin pain.', 'USS was able to detect tendon thinning, signal abnormality, and ossification (c) but a larger cohort would be needed to accurately interpret the diagnostic sensitivity.']","Figure 2. Case 2. Radiography, MARS MRI, ultrasound, and intraoperative images of gluteal musculotendinous damage. a. A pelvic radiograph showing a left-sided MOM total hip replacement in situ and highlighting the absence of the greater trochanter region of the left proximal femoral bone. b. A T1-weighted MARS MRI image in coronal section showing left-sided fatty atrophy of the gluteus medius and gluteus minimus muscles (grade 3) and thinning of the gluteus minimus tendon. c. Left lateral USS over the greater trochanter showing thin and hypoechoic tendons for the gluteus medius and gluteus minimus muscles. d. Photograph taken during revision surgery showing erosion of the left greater trochanter and gluteus medius muscle. MOM GT: the MOM femoral component (greater trochanter region); Gmed: gluteus medius tendon. Pathology is indicated by white arrows.",yes
PMC11508844,Figure_5,oa_package/98/8f/PMC11508844.tar.gz,"['However, TEE imaging is helpful in cases of suboptimal or insufficient TTE imaging for diagnosis and is necessary for therapeutic planning ().', 'Tricuspid regurgitation and cardiac implantable electronic devices (CIED).']","Figure 3 Coaptation gap measurement on a multiplanar reconstruction of a 3D trans-esophageal echocardiography dataset red, blue and green lines represent orthogonal reference planes ( ); biplane commissural view ( ); transgastric en-face view ( ).",yes
PMC6048663,Figure_5,oa_package/07/72/PMC6048663.tar.gz,"['\nA compares the mean\nreconstructed Raman spectra of normal tissue (blue line), invasive breast carcinoma (red\nline), and fibroadenoma (black line) and assigns to the Raman signatures of their molecular origin.', 'B shows in the form of\na difference spectrum where the main differences between the Raman spectra of fibroadenoma\nand an invasive breast carcinoma can be found.', '.', '1177_1533033818782532-fig5""/>Table 1.', 'Considering the reconstructed Raman spectra shown in A, several clear spectral features\ndifferentiate between normal breast tissue and tumor tissue, irrespective of whether the\ntumor is fibroadenoma or an invasive breast carcinoma.', 'However, the differences between the 2 tumor tissues, fibroadenoma and the invasive\ncarcinoma, are significantly less pronounced but are made visible in the difference spectrum\nin B.', 'Considering the reconstructed Raman spectra shown in A again, criteria for the differentiation\nbetween fibroadenoma and invasive carcinoma could be for example (1) the small shift of the\nCH3 and CH2 deformation band of protein from 1451 to 1454\ncm 1 (2) or at 1301 cm 1, the lipid peak of the invasive breast\ncarcinoma, which we did not see in the spectra of fibroadenoma.', 'Besides, they showed strong presence of protein\nbands (1246, 1455, and 1656 cm 1) in fibroadenoma than in invasive carcinoma\nwhich reflects our findings in \n5B.']","Figure 5. A, Mean reconstructed Raman spectrum of normal tissue (blue line), invasive carcinoma(red line), fibroadenoma (black line), and formalin (thin gray line) with peak positionassignment to their respective molecular origin. B, Difference spectrum when the meanRaman spectrum of fibroadenoma is subtracted from the mean Raman spectrum of invasivebreast carcinoma.",yes
PMC4980423,Figure_5,oa_package/46/06/PMC4980423.tar.gz,"[' 5).', 'AVM with occluded channels and channels with signs of recanalization and neoangiogenesis; surrounding gliosis is also remarkable (Masson trichrome elastica, magnification 40 )Histology sample 2.']","Fig. 5 Histology sample 1. AVM with occluded channels and channels with signs of recanalization and neoangiogenesis; surrounding gliosis is also remarkable (Masson trichrome elastica, magnification 40)",yes
PMC10503948,Figure_1,oa_package/0a/eb/PMC10503948.tar.gz,['T1-weighted image of MRI thoracic spine with enhancing posterior epidural mass spanning from T1-2 to T3-4.'],Figure 1 T1-weighted image of MRI thoracic spine with enhancing posterior epidural mass spanning from T1-2 to T3-4.,yes
PMC8642678,Figure_1,oa_package/88/8b/PMC8642678.tar.gz,[],"FIGURE 1 (A) Light microscopy of porcine progenitor cells at second passage isolated from (a) pericard, (b) pleura, (c) adipose tissue and (d) trachea. Original magnification 100. (B) Characterization of cells from porcine pericard, pleura, trachea and adipose tissue. Relative expression of mesenchymal stem cell markers CD73, CD90, CD105, CKIT and SOX9 using RTPCR. No significant differences between groups were observed ( <0.05) N=6.",yes
PMC6546218,Figure_3,oa_package/fb/85/PMC6546218.tar.gz,"['Comparison of G0 and G2 exposed podocytes identified 7523 differentially expressed transcripts (A, red dots).', 'Of these transcripts, approximately the same number were found to be up-regulated (A, red points above y = 0, n = 3943) as down-regulated (A, red points below y = 0, n = 3580) in G2 exposed podocytes.', 'Of the 107 transcripts significantly perturbed in endothelial cells, two-thirds (72 of 107) also showed perturbed expression in podocytes (B).', 'g003Comparison of G0 and G2 exposed cells.']",10.1371/journal.pone.0217042.g003,yes
PMC10161303,Figure_2,oa_package/d7/d5/PMC10161303.tar.gz,"['\n10\n.', '1177_20503121231170478-fig2"" position=""float""/>.']",Figure 2. (a) DSA showing angioma in the sphenopalatine artery in a patient suffering from Rendu-Osler-Weber-Syndrome. Anteroposterior view before embolization (arrow pointing at the angioma); (b) DSA showing angioma in the sphenopalatine artery in a patient suffering from Rendu-Osler-Weber-Syndrome. Lateral view before embolization (arrow pointing at the angioma); (c) DSA showing angioma in the sphenopalatine artery in a patient suffering from Rendu-Osler-Weber-Syndrome. Anteroposterior view after embolization; (d) DSA showing angioma in the sphenopalatine artery in a patient suffering from Rendu-Osler-Weber-Syndrome. Lateral view after embolization. DSA: Digital subtraction angiography.,yes
PMC8844236,Figure_2,oa_package/23/f8/PMC8844236.tar.gz,"['819) ().', '001"" position=""float""/>Ultrasonography of the nodules in papillary thyroid carcinoma (PTC) without central lymph node metastasis.']","Figure 2 Ultrasonography of the nodules in papillary thyroid carcinoma (PTC) without central lymph node metastasis. The tumor is located in the middle of the left thyroid lobe, with a maximum diameter of 8mm, solid and very hypoechoic, aspect ratio >1, and unclear margin. The American College of Radiology Thyroid Imaging Reporting and Data System (ACT TI-RADS) score was 8 points. (a) Transverse section; (b) longitudinal section.",yes
PMC8262360,Figure_6,oa_package/82/53/PMC8262360.tar.gz,"['01; , A C).', ')Western blot markers for the amyloidogenic cascade.']","Figure 6 Western blot markers for the amyloidogenic cascade. ( ) BACE1 in the prefrontal cortex and hippocampus as well as representative blots; ( ) total APP in the prefrontal cortex and hippocampus as well as representative blots; ( ) CTF in the prefrontal cortex and hippocampus as well as representative blots. Data analyzed using an unpaired, 2-tailed test. Values are represented as mean SEM. The box plots depict the minimum and maximum values (whiskers), the upper and lower quartiles, and the median. The length of the box represents the interquartile range. Significance indicated by * < 0.05 compared with control. (CON; = 5, WD-AB; = 4.)",yes
PMC8551647,Figure_2,oa_package/13/4f/PMC8551647.tar.gz,[''],Fig. 2 A & 2B: Clinical presentations of one patient with PV before and after 3-month treatment with rituximab.,yes
PMC7993245,Figure_15,oa_package/e0/5d/PMC7993245.tar.gz,[],Figure 4e: Examples of image-level annotations on axial CT images indicated with orange regions of interest. Infectious opacity segmented in the left upper lobe. Infectious tree-in-bud and/or micronodules segmented in the right lower lobe. Infectious cavity segmented in the right upper lobe. Noninfectious nodule or mass segmented in the posterior left pleura. Atelectasis segmented in the left lower lobe. Other noninfectious opacity segmented in the right lower lobe.,yes
PMC3420691,Figure_2,oa_package/da/5e/PMC3420691.tar.gz,"['Malignant cells were infiltrating between pre-existing benign ducts in the periphery of the tumor ().', '001""/>A remaining breast duct in the periphery of the tumor, immunoreactive to keratins AE1/AE3, surrounded by compactly arranged malignant cells, negative to keratins (DAB/Hematoxylin, 400).']","Figure 2 A remaining breast duct in the periphery of the tumor, immunoreactive to keratins AE1/AE3, surrounded by compactly arranged malignant cells, negative to keratins (DAB/Hematoxylin, 400).",yes
PMC4165778,Figure_4,oa_package/a1/41/PMC4165778.tar.gz,"[""T1-weighted images (T1WI) of mice bearing breast cancer xenografts in all four treatment groups, demonstrating homogeneous hypointense tumor masses (arrows) on the animals' unilateral back.""]","Figure 4 T1-weighted images (T1WI) of mice bearing breast cancer xenografts in all four treatment groups, demonstrating homogeneous hypointense tumor masses (arrows) on the animals' unilateral back. The tumor masses become hyperintense after intravenous administration of gadolinium (enhanced T1WI). The follow-up imaging of tumor growth at different time points shows that tumor size in the chemo plus radiofrequency heat (RFH) group (s-x) clearly decreases at week 2 after treatment (arrow on x), in comparison to those in the control (a-f), RFH-only (g-l), and chemo-only (m-r) groups.",yes
PMC10297602,Figure_25,oa_package/54/6e/PMC10297602.tar.gz,[],"Figure 25 Sagittal T1 ( ) and T2 ( ) images of an eosinophilic granuloma of a mid-thoracic vertebra (white arrows). The signal characteristics are usually non-specific, with lesions appearing hypo to isointense on T1 and hyperintense on T2. Pathological collapse, as seen here, is common.",yes
PMC10555573,Figure_1,oa_package/33/99/PMC10555573.tar.gz,"['Ten years earlier, he had undergone a lumpectomy for MPNST in the left lower leg (A).', 'Contrast-enhanced magnetic resonance imaging (MRI) revealed intracranial enhanced mass lesions in the right cerebellum, left insular gyrus, and left superior parietal lobule (B).', 'The tumor consisted of spindle-shaped mesenchymal cells growing in a fascicular or storiform fashion with foci of geographic necrosis (C and D).']","FIG. 1. : Axial postcontrast T1-weighted MRI showing a heterogeneous enhanced mass lesion in the left lower leg ( ). Axial postcontrast T1-weighted MRI showing a heterogeneous enhanced mass lesion in the cerebellum ( ). Histopathological findings of the resected specimen. Spindle-shaped malignant cells grow in a fascicular or storiform fashion, with foci of necrosis, consistent findings with MPNST. Hematoxylin and eosin (H&E), original magnification 40 ( ); H&E, original magnification 200 ( ).",yes
PMC4332639,Figure_3,oa_package/ff/10/PMC4332639.tar.gz,"['Shoulder radiographs revealed an avulsion fracture of the lesser tuberosity ().', '.', '1177_1941738114533657-fig3""/>.']",Figure 3. Axillary view of case 2 demonstrating avulsion fracture of the lesser tuberosity.,yes
PMC9499725,Figure_1,oa_package/e4/f0/PMC9499725.tar.gz,"['An additional finding was observation of increased dimensions of the right lobe of the thyroid, which projected inferiorly to the anterior mediastinum, displacing the trachea to the left ().', 'Computed tomography showing: (A) tumor in intimate contact with the posterolateral portion of the right internal jugular vein, esophageal wall, and right sternocleidomastoid muscle (axial slice); and (B) the same tumor and its relationship to the convergence of the right internal jugular vein and the right subclavian vein (coronal slice).']","Figure 1 Computed tomography showing: (A) tumor in intimate contact with the posterolateral portion of the right internal jugular vein, esophageal wall, and right sternocleidomastoid muscle (axial slice); and (B) the same tumor and its relationship to the convergence of the right internal jugular vein and the right subclavian vein (coronal slice).",yes
PMC4973052,Figure_6,oa_package/9d/f5/PMC4973052.tar.gz,"[' 6).', '', 'Multiple hypo enhancing splenic lesions were seen (curved arrow), likely representing hamartomas as well as subcentimeter fat density lesions within the pancreas (average HU = 70) (straight arrows)Following neoadjuvant chemotherapy, our patient underwent a right modified radical mastectomy with axillary lymph node dissection.']","Fig.6 Axial contrast enhanced CT of the Abdomen. Multiple hypo enhancing splenic lesions were seen ( ), likely representing hamartomas as well as subcentimeter fat density lesions within the pancreas (average HU=70) ( )",yes
PMC7694731,Figure_6,oa_package/f1/b3/PMC7694731.tar.gz,"['Two-stage urethroplasty for excision and reconstruction of the strictures and diverticulum was undertaken [].', '(A-C)Intraoperative photographs and diagram of surgical management in the 45-year-old male patient, diagnosed with right PUJ calculus, complicated by post-obstructive right renal atrophy, urethral strictures, and diverticulum (A) excision of the diverticulum; (B) urethroplasty performed using buccal mucosa re-inforcement; (C) line diagram representing the surgical technique used.']","Figure 6( Intraoperative photographs and diagram of surgical management in the 45-year-old male patient, diagnosed with right PUJ calculus, complicated by post-obstructive right renal atrophy, urethral strictures, and diverticulum (A) excision of the diverticulum; (B) urethroplasty performed using buccal mucosa re-inforcement; (C) line diagram representing the surgical technique used.",yes
PMC3691657,Figure_2,oa_package/e0/14/PMC3691657.tar.gz,['Immunoblot analysis confirms changes in protein expression of CYB5A.'],"Figure 2 representative western blot analysis of CYB5A in HCC, fibrotic liver and HepG2 cell line. For this, 100g of nuclear membrane samples were loaded onto a 12% SDS-PAGE. Expression level was analyzed using the primary antibody CYB5A (1:1000 dilution) and horse raddish peroxidase (HRP) - conjugated secondary antibody (1: 5000 dilution). -actin and GAPDH were used as a loading control. representative graphical expression of validated proteins by western blotting. Digital images were taken by gel documentation system (Bio-Rad). Quantification and intensity measurement of protein bands were analyzed by Quantity One gel analysis software (Bio-Rad). Statistical significance (p- value > 0.05) was calculated using SPSS statistics version 17.",yes
PMC2873913,Figure_6,oa_package/17/2a/PMC2873913.tar.gz,['g006Spleen is required for the onset of malaria-associated ALI.'],10.1371/journal.ppat.1000916.g006,yes
PMC2614950,Figure_2,oa_package/f2/c6/PMC2614950.tar.gz,['Pre-operative coronal computer tomography image of the left shoulder.'],Figure 2 Pre-operative coronal computer tomography image of the left shoulder. It shows the fracture of the acromion with an irregular margin and hypertrophy at the superior aspects of the bony ends. The size of the fragment can also be appreciated.,yes
PMC11502597,Figure_2,oa_package/6a/f5/PMC11502597.tar.gz,"[' 2).', 'Abb.', '2Postmortale Computertomographie (PMCT), Gas im subkutanen Weichteilgewebe (rote Pfeile) bei offener Hautverletzung (gelbe Umkreisung) am Oberschenkel nach Kollision als Fahrradfahrerin mit AutoVon forensischer Relevanz sind zudem sog.']","Abb. 2 Postmortale Computertomographie (PMCT), Gas im subkutanen Weichteilgewebe( ) bei offener Hautverletzung( ) am Oberschenkel nach Kollision als Fahrradfahrerin mit Auto",yes
PMC10402191,Figure_2,oa_package/c2/34/PMC10402191.tar.gz,"['To evaluate this, we utilized an established multi-color flow cytometry panel identifying SSCs18 to analyze skeletal stem/progenitor cells populations in long bones of Col1a2oim/oim mice (A).', 'Interestingly, the abundance of immunophenotypic SSCs was elevated in Col1a2oim/oim mice, yet the amounts of pre-bone cartilage skeletal progenitors (pre-BCSPs) and bone cartilage skeletal progenitors (BCSPs), SSC-derived non-stem progenitors17, was unchanged (B).', 'Consistent with this, the number of CD200 positive cells was obviously increased in the primary spongiosum near the growth plate in Col1a2oim/oim mice, a region characterized as housing more SSCs and more artery/arterioles16,17,27,28 (C).', 'Moreover, delayed osteogenesis attributed to impaired mineralization was observed in Col1a2oim/oim mice evidenced by the whole-mount skeletal staining in neonatal mice (D), with the early emergence of this phenotype being consistent with alterations in the early SSC compartment.', 'Scale bars, 100 mElevated abundance of skeletal stem cells in Col1a2oim/oim mice(A) Flow cytometry gating strategies for analysis of SSC, pre-BCSP and BCSP proportions.']","Figure 2 Elevated abundance of skeletal stem cells in mice (A) Flow cytometry gating strategies for analysis of SSC, pre-BCSP and BCSP proportions. (B)Dot plot for analysis of SSC, pre-BCSP and BCSP proportions in mice and littermate controls. Results represented as mean s.e.m.; *P < 0.05 by an unpaired two-tailed Students t-test in all panels. (C)Representative confocal images (n = 3 total images per group) of femur sections from 3-week-old and male mice stained with CD200 (red) OSX (green) and DAPI (blue). The growth plate is marked with a dashed line. (Top, lower power; Bottom, higher power). Scale bars, 250 m (low power) and 100m (high power). (D)Alcain blue and alizarin red staining of skeletal preparations of newborn mice at postnatal day 3. Scale bars, 250 mm",yes
PMC7666849,Figure_4,oa_package/67/bb/PMC7666849.tar.gz,"['\n\nDifferent kind of splenic tissue alterations are illustrated in: A) Venous sinuses congestion in the red pulp, B) Lymphoplasmacytic cell infiltration in the red pulp, and C) White pulp hyperplasia.']","Figure 4 Different kind of splenic tissue alterations are illustrated in: Venous sinuses congestion in the red pulp, Lymphoplasmacytic cell infiltration in the red pulp, and White pulp hyperplasia. Hematoxylin and eosin stain.",yes
PMC7647562,Figure_3,oa_package/d4/da/PMC7647562.tar.gz,['Hematoxylin-eosin [HE] neuroblast-like clusters of small round cells are present in the fibrous tissue with melanin pigmentation.'],"Figure 3 Hematoxylin-eosin [HE] neuroblast-like clusters of small round cells are present in the fibrous tissue with melanin pigmentation. Immunohistochemical staining revealed positive expression for(C) cytokeratin, (D) EMA, (E) HMB-45, (F) neuron-specific enolase, (G) SYN, and (H) VIM. Glial fibrillary acidic protein (GFAP), S100, CD99, DES, LCA, SOX10, SMA, TDT, and CD34 were negative (data not shown).",yes
PMC6800937,Figure_6,oa_package/14/25/PMC6800937.tar.gz,"['The field of redox biology has extensively addressed the effect of ROS in cell membranes; for that reason, this section will put the effects of plasma treatments on cell membrane components in context with the current knowledge in redox biology ().', '005""/>The cell membrane is the key compartment that plasma-derived ROS need to penetrate or interact with to elicit biological responses.']","Figure 6 The cell membrane is the key compartment that plasma-derived ROS need to penetrate or interact with to elicit biological responses. While some ROS are able to penetrate cellular membranes (e.g., ozone, nitric oxide and atomic oxygen), other more polar ROS cannot (e.g., singlet delta oxygen, nitrite, hydroxyl radical, superoxide anion, hydrogen, and peroxynitrite). Hydrogen peroxide is actively transported into the cells via transporters such as aquaporins.",yes
PMC3303905,Figure_26,oa_package/ba/7f/PMC3303905.tar.gz,[],Fig. 26 Lung biopsy through non-aerated route using mediastinal fat as window. Axial CT image of 68-year-old man shows left upper lobe mass which was abutting mediastinal fat. Biopsy needle was advanced from left parasternal approach lateral to internal thoracic vessels (arrow) using mediastinal fat as window. CT image at caudal level showed needle entering into mass without transgression of aerated lung. Biopsy results were suggestive of adenocarcinoma.,yes
PMC8718151,Figure_5,oa_package/41/1c/PMC8718151.tar.gz,"['In addition, similar patterns of neutrophil and monocyte counts were observed in response to myocardial I/R (, A F).', 'There was no difference in cardiac function 1 week after MI among those that survived (Supplemental , B F).', 'GSDMD is essential for recruitment of neutrophils/monocytes to the I/R heart.']","Figure 5 GSDMD is essential for recruitment of neutrophils/monocytes to the I/R heart. ( ) Flow cytometric analysis and quantification of Cd11b Ly6G neutrophils and Cd11b Ly6C monocytes in heart ( and ), blood ( and ), or bone marrow (BM) ( and ) from WT or mice at different reperfusion time points (3 hours, 6 hours, 12 hours, 24 hours) after I/R or sham surgery. Corresponding values are indicated in the plots. The statistical significance of sham versus 3, 6, 12, or 24 hours is indicated ( 35). ( and ) Flow cytometric analysis ( ) and quantification ( ) of Cd11b Ly6G neutrophils and Cd11b Ly6C monocytes in heart (left), blood (middle), or BM (right) from WT or mice 24 hours after I/R ( = 3). Data are presented as mean SD. * 0.05; ** 0.01; *** 0.001; **** 0.0001 by 1-way ANOVA followed by Bonferronis multiple-comparison test ( , , and ) or multiple 2-tailed Students test ( ). NS, not significant.",yes
PMC9379723,Figure_1,oa_package/f7/bd/PMC9379723.tar.gz,"['Lumbar computed tomography (CT) myelography confirmed the presence of severe left foraminal stenosis at L5 S1 (A C).', '3171/CASE21566"" ext-link-type=""uri"">Supplementary ).']",FIG. 1. Sagittal lumbar CT myelogram revealing severe left foraminal stenosis ( ) compressing the L5 nerve root. Midsagittal view showing severe central spinal stenosis ( ) at L45. Axial view revealed severe left foraminal stenosis ( ).,yes
PMC6554298,Figure_4,oa_package/8b/bd/PMC6554298.tar.gz,"[' 4) are internally consistent with those of ', 'Both constitutive and estrogen-enhanced growth of ESR mutations is dependent upon ER signaling.', 'We next determined if the loss of E2-enhanced growth for the Y537S mutant at zone 3(3 6%) oxygen tension could be rescued by return to culture at zone 1 (12 15%) oxygen levels in both co-culture and LAMPS models.', ' 4).']","Figure 4 Both constitutive and estrogen-enhanced growth of ESR mutations is dependent upon ER signaling. ESR1-expressing cells were grown without (solid bars) or with 5nM E2 (hashed bars) in co-culture, and were maintained at 20% ( ) or 5% ( ) oxygen for 13 days . Cells were treated with vehicle (blue bars) or ER antagonists 1M fulvestrant (red bars) or 1M AZD9496. Graphs are plotted as the mean change in fluorescence intensitySD of three fields. Experiments were repeated 3 times (n=3) per condition. For E2-dependent growth of individual clones maintained at Zone 1 oxygen, an unpaired, 2-tailed -test (black asterisks; ) produced significant p-values (p<0.05) for WT (p=0.008) and Y537S (p=0.01), while no significant increases were observed for clones at Zone 3 oxygen (B). A one-way ANOVA was used to compare constitutive growth of all clones at Zone 1 oxygen ( ) [red asterisks; p=0.01] and Zone 3 oxygen ( ) [red asterisks; p=0.02]. Similar analysis for clones treated with E2 produced significant p-values at Zone 1 oxygen (A; p=0.02) as well as cells maintained at Zone 3 oxygen (B; p=0.03). ANOVA analysis comparing WT clones treated with E2 compared to the constitutive growth of ESR1 mutants did not result in a significant difference at Zone 1 oxygen; ( ) however, for cells maintained at Zone 3 oxygen tension ( ), a significant difference was observed (p=0.04). For cells maintained at 20% oxygen, in addition to significant constitutive growth observed for both mutants, Y537S mutants confer enhanced estrogen-dependent growth, consistent with the results in Fig. . The constitutive and estrogen-enhanced growth for both ESR1 mutants is inhibited by ER antagonist treatment, indicating dependency on ER signaling. As a control, estrogen-dependent growth of WT clones is also inhibited by ER antagonist treatment. For cells maintained at 5% oxygen ( ), enhanced estrogen-dependent growth is lost for Y537S mutants, consistent with previous results; however, growth similar to constitutive levels is maintained and is inhibited with ER antagonist, indicating dependency on ER signaling. Although constitutive and E2-dependent growths requires ER signaling, only the E2-dependent growth is regulated by oxygen tension in co-culture and LAMPS.",yes
PMC8615201,Figure_4,oa_package/57/be/PMC8615201.tar.gz,"['AngII-treated Apoe / Light / mouse AAA displayed decreased mRNA levels of Acta2, Col1a1, and Opn and augmented expression levels of the osteochondrogenic marker Sox9 without changes in the gene expression of Tgfb1, Klf4, Sox2, Oct4, Ckit, Sca, and Bmp2 (a).', 'Both AngII-treated Apoe / Light / and Apoe / mice displayed diminished mRNA levels of Acta2 and Tgfb1 genes (b) compared with vehicle-treated mice.', 'In addition, AngII-treated Apoe / Light / mice showed reduced expression of Klf4 and Sox2, while only AngII-treated Apoe / mice exhibited decreased Oct4, Bmp2, along with augmented Opn mRNA levels compared with vehicle counterparts (b).', 'Comparison between the two vehicle-treated mice groups revealed diminished Tgfb1 and Oct4 gene expression levels and augmented Acta2 mRNA levels in Apoe / Light / mice compared with vehicle-treated Apoe / mouse controls (b).', 'Gene expression analysis in abdominal aortic tissue in vehicle- and AngII treated Apoe / and Apoe / Light / mice.']","Figure 4 Gene expression analysis in abdominal aortic tissue in vehicle- and AngIItreated and mice. mRNA levels of , and in ( ) AngII-treated mice and in ( ) all four mouse groups. mRNA levels were normalized to mRNA levels and relativized to ( ) AngII-treated Apoe mice or to ( ) vehicle-treated Apoe mice. Statistical analyses were: ( ) Students -test and MannWhitney U test ( ) and ( ) Two-way ANOVA followed by Tukeys multiple comparison test. Data are mean SEM. * 0.05, ** < 0.01 *** < 0.001, and **** < 0.0001.",yes
PMC3799706,Figure_3,oa_package/bf/4d/PMC3799706.tar.gz,"['In addition to dilated loops of bowel, the whirlpool sign (bowel loops with accompanying mesentery and vessels wrapping around the main SMA) and the coffee bean or kinked loop sign (which is a distention of a very short segment of bowel, which resembles a coffee bean) are signs of pathognomonic for intestinal volvulus ().', '8 Coffee bean sign at 36 weeks of gestation sonogram.']",Fig. 3 Coffee bean sign at 36 weeks of gestation sonogram.,yes
PMC6098669,Figure_2,oa_package/ee/bf/PMC6098669.tar.gz,['CCA common carotid asrtery; IJV internal jugular vein; LN lymph nodeContrast enhancement image.'],"Figure 2 Contrast enhancement image. The enhanced pattern of the enlarged lymph node (arrow indicates the enhanced lymph node) is homogeneous and intense. TTP=17 s, I=18.",yes
PMC4750554,Figure_1,oa_package/9b/59/PMC4750554.tar.gz,"['The recorded craniocaudal diameter was primarily derived from the macroscopy report and verified by the photograph of the specimen and, if the cervix was embedded completely, from the microscopy report (c).', '5Evaluation of MRIBefore evaluation, a case record form (CRF) was developed including instructions on how to perform the measurements, based on consensus between the two radiologists and the investigator, with specific attention paid to how craniocaudal measurements were spatially made in histopathology (a).', 'The radiologists had knowledge of the patient s medical history up until the time MRI was performed and were blinded to the histopathology findings (b).', '420133124392245(A) Craniocaudal tumour extension of the primary tumour measured parallel to the endocervical channel in sagittal plane.']",Fig. 1 (A) Craniocaudal tumour extension of the primary tumour measured parallel to the endocervical channel in sagittal plane. (B) Example of measuring craniocaudal tumour extension in a 51-year-old woman with FIGO stage IB1 uterine cervical cancer in sagittal plane on MRI and on pathology (C). The two-headed arrow indicates the cranial and caudal extension.,yes
PMC11122514,Figure_5,oa_package/27/6a/PMC11122514.tar.gz,"['For a better assessment of tumor thickness and a clear distinction from other histologic mimics, an immunohistochemical study was performed, which revealed positive markers: SOX10 and Melan A ().', 'Immunohistochemical expression of SOX 10 in the melanocytic lesion (4 ).']",Figure 5 Immunohistochemical expression of SOX 10 in the melanocytic lesion (4).,yes
PMC8604993,Figure_5,oa_package/0e/f5/PMC8604993.tar.gz,"[' 5A) and MDA (51.', ' 5B).', ' 5C).', 'Effects of MAP, L.', '(A) Effect of MAP infection on oxidative stress level in Caco-2 monolayers in the presence of excess level (500 ng/mL) of 5-HT following 24 h of incubation.']","Figure 5 Effects of MAP, and following 24h of infection on LDH Activity ( ) GSH Activity ( ) and MDA level ( ) in fully differentiated Caco-2 monolayers in the presence of different levels of 5-HT (0, 100, 250, and 500ng/mL) following 24h of incubation. LDH and GSH activity values are presented as percentage activity of control group without infection or 5-HT treatment. Values were pre-tested for normal distribution using the KolmogorovSmirnov normality test. Significance among experiments was assessed by repeated measures analysis of variance (ANOVA) followed by Bonferroni correction test. All LDH, GSH and MDA activity experiments were performed in triplicates. Data are presented as MeanSD. * value<0.05.",yes
PMC8473128,Figure_2,oa_package/c5/38/PMC8473128.tar.gz,"['The difference in the medians between the recovered and deceased patients was statistically significant (a).', '6), and a significant difference between the two groups was observed (Table 2; b).', 'The median lymphocyte count was statistically significantly higher in the recovered patients than in the deceased patients (c).', 'The median eosinophil count in the recovered patients was significantly higher than the median in the deceased patients (d).', 'There was a significant difference between the groups (e).', '36 109/L), with no difference between the groups (f).', 'Therefore, in contrast to the monocyte counts, the NLR values and neutrophil, lymphocyte, eosinophil, and basophil counts were significantly different between the groups of patients (a f).', 'The eosinophil counts showed the greatest difference between the recovered and deceased patients compared to the other types of leukocytes (b f).', 'These assertions are based on:\n(1)The fact that, on average, the eosinophil counts were higher in the recovered than in the deceased patients (d);(2)Although both groups of patients presented eosinophil count values of zero in one or more of their WBC differential counts, this situation was more frequently observed in the deceased than in the recovered patients.', 'org/1999/xlink"" xlink:href=""viruses-13-01675-g001"" position=""float""/>Comparison of (a) neutrophil-to-lymphocyte ratios (NLRs) and (b f) white blood cell WBC differential counts of COVID-19 patients with different outcomes.']",Figure 2 Comparison of ( ) neutrophil-to-lymphocyte ratios (NLRs) and ( ) white blood cell WBC differential counts of COVID-19 patients with different outcomes. The median values of 152 WBC differential counts with their respective NLRs from 59 recovered patients were compared with the median values of 126 WBC differential counts and NLRs from 60 deceased patients. Bars indicate standard errors. The dashed lines indicate the means of normal values of NLRs or leukocytes. The analysis was performed using the Wilcoxon matched-pairs test.,yes
PMC34117,Figure_4,oa_package/53/e5/PMC34117.tar.gz,"['Dual color IHC (HLA-DR peroxidase/CD8 FITC) viewed in dark field visible light (A), incident fluorescence (B) and dark field fluorescence (C).']","Figure 4 Dual color IHC (HLA-DR peroxidase/CD8 FITC) viewed in dark field visible light (A), incident fluorescence (B) and dark field fluorescence (C). ) Interface (dashed line) between pb and im layers. White arrowhead shows differentiating DC, yellow arrowhead shows mature DC. Arrow indicates activated T cell with HLA-DR expression (see below). ) White arrow indicates T cell exhibiting unusual elongated shape at the interface. Yellow arrows indicate residual CD8 expression in fragmented T cell among adjacent im epithelial cells. C) Activated T cell with HLA-DR expression (white arrow) interacts with differentiating DC (white arrowhead). Mature DC (yellow arrowhead) accompany T cell fragmentation (yellow arrows).",yes
PMC4629303,Figure_4,oa_package/3a/9e/PMC4629303.tar.gz,"['Also, no differences were observed within the combined regional and global cerebral blood flow levels at all the measured time points in sham rats [e].', '(a-e) Charts show temporal cerebral blood flow changes in different brain regions of sham rats.']","Figure 4 (a-e) Charts show temporal cerebral blood flow changes in different brain regions of sham rats. In sham rats, no apparent differences were observed in the cerebral blood flow (CBF) values at various time points in the structures analyzed.",yes
PMC4165629,Figure_1,oa_package/32/ef/PMC4165629.tar.gz,"[') technology was used to confirm the level of the tumor in the pelvis throughout the whole procedure ().', '0-84862579282Pelvic MRI of the patient.']",Figure 1 Pelvic MRI of the patient. The arrow demonstrates the pararectal GIST.,yes
PMC4666898,Figure_2,oa_package/c7/b8/PMC4666898.tar.gz,"[' 2) is proposed as a guide to decide about the necessary or unnecessary imaging steps.', 'Recommendation for diagnostic imaging in children with disorder of sexual developmentImaging in childhood testicular torsionThere are two age-dependent peaks for testicular torsion: perinatally (within the first month of life; usually occurring prenatally or during birth) and in older children.']",Fig. 2 Recommendation for diagnostic imaging in children with disorder of sexual development,yes
PMC4048180,Figure_3,oa_package/49/7a/PMC4048180.tar.gz,"['(A).', 'rodentium in the spleen, we also observed significant splenomegaly in Nlrp3 / and Asc / mice, but not in WT mice (B).', 'Splenomegaly was associated with greater accumulation of granulocytes (CD11b+GR1high) in Nlrp3 / and Asc / spleens compared to WT spleens (C, D).', '001)C.']","Figure 3 infected and mice cannot restrict bacterial translocation and display systemic inflammation WT, , and mice were orally infected with ~ 10 and sacrificed 8 or 14 days p.i. (A) Splenic bacterial loads 8 days p.i. (B) Spleen weights (C) Representative FACS plots of splenic granulocytes (CD11b Gr1 ) (D) Frequency of granulocytes in the spleen (CD11b Gr1 ) Each symbol represents a single animal and data represent pooled results from at least two independent experiments ( = 9-20). Horizontal bars represent group medians. Statistical significance was determined by the Mann-Whitney test (* = P < 0.05; ** = P < 0.01; *** = P < 0.001)",yes
PMC10469149,Figure_1,oa_package/8a/e2/PMC10469149.tar.gz,"['The right lobe was completely replaced by a spongiform nodule, with increased vascularity (), which was classified as benign by ACR-TIRADS (score 2) (15).', '1349629029377Right thyroid lobe is replaced by a spongiform nodule (transverse plane).']",Figure 1 Right thyroid lobe is replaced by a spongiform nodule (transverse plane).,yes
PMC7205220,Figure_4,oa_package/04/ea/PMC7205220.tar.gz,"['g003""/>We present statistical analyses of the depth of AVNs in .', 'A provides a summary of aligned AVN minimum depth topology.', 'B details AVN depths across the hearts using box plots indicating depth mean and quartile ranges.', 'e002"">Eq 2) on data presented in B revealed an increase in nodal minimal depth with age (R2 = 0.', '018) (C).', 'g004Depth analyses of AVN-His bundle complex.', 'g004""/>Depth analyses of AVN regions are presented in D 4I.', 'D and 4G show depth in the AVN extension region and a logarithmic regression model for the relationship between depth minima and age, respectively.', 'The regression analysis of G did not yield a significance difference to a constant model (P 0.', 'E and 4H show the depth and logarithmic regression analysis of the compact node region, respectively.', 'F and 4I detail the depth range and logarithmic regression of the transition to His bundle region.', 'The analyses yielded a similar depth distribution from the AVN extensions to His bundle across all ages (A).', 'Our work revealed that minimum AVN depth increases logarithmically with age ().', 'This increase in depth occurred for the complete node (C) and in the compact region.']",10.1371/journal.pone.0232618.g004,yes
PMC9723313,Figure_1,oa_package/42/2e/PMC9723313.tar.gz,"['Because this patient population is characterised by increased cortical and medial temporal lobe atrophy and higher white matter hyperintensities load, we identified two analysis steps that required optimisation ().', 'Results of UKB pipeline optimisation for memory clinic population.', 'See Table 3 for original counts for each score and supplementary for boxplots with original scores).']","Fig. 1 Results of UKB pipeline optimisation for memory clinic population. a) Example T1-weighted scan from a BHC patient where WMHs are T1 hypointense, similar in intensity to grey matter (GM). b) Corresponding T2-FLAIR scan showing WMHs. c) Uncorrected GM segmentation (red), with significant periventricular and deep WMHs classified as GM (white arrows). d) GM segmentation corrected with lesion-masking (green). e) Example T1-weighted scan from a different BHC patient, f) zoomed-in around the hippocampus. g) Uncorrected hippocampus segmentation (red) with errors (inclusion of CSF) highlighted by white arrow. h) CSF-masked hippocampus segmentation removed incorrectly labelled CSF voxels. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)",yes
PMC9663268,Figure_6,oa_package/da/26/PMC9663268.tar.gz,['Histopathology microscopic examination consistent with synovial chondromatosis.'],Figure 6 Histopathology microscopic examination consistent with synovial chondromatosis.,yes
PMC8554444,Figure_3,oa_package/e5/16/PMC8554444.tar.gz,['\nPre-operative contrast-enhanced pelvic magnetic resonance imaging.'],"Figure 3 The orange arrow indicates a space-occupying lesion seen on the left wall of the bladder, originating from the bladder wall mucous membrane. It tended to infiltrate peripheral tissue, which was preliminarily suspected to be a desmoplastic fibroma or leiomyoma. A-D: It shows an oval structure that is isointense on T1WI (A) and T2WI (B) sequences; T1WI + fat suppression + enhanced sequence reveals noticeable enhancement (C); DWI sequence revealed limited diffusion (D).",yes
PMC9539766,Figure_9,oa_package/cd/b3/PMC9539766.tar.gz,[],FIGURE 9 The effect of OMT on the cell momhology of LPS-induced RAW264.7 cell inflammation model. Each group was visualized by an ordinary light microscope ( 400 magnification).,yes
PMC3278928,Figure_22,oa_package/5f/d4/PMC3278928.tar.gz,[],"Figure 22 Nucleotide composition of U1 5 oligonucleotide [ ]. The peak from the GTP region was digested with snake venom phosphodiesterase and separated on Whatman 3MM paper by electrophoresis at pH 3.5 (5% acetic acid adjusted pH to 3.5 with ammonium hydroxide) and chromatography in the second dimension with a solvent system consisting of isopropyl alcohol, HCl, and H O in the ratio of 680:176:144 by volume. Autoradiography was performed using X-ray film.",yes
PMC7972297,Figure_2,oa_package/4e/9e/PMC7972297.tar.gz,[],"FIGURE 2 A 7-year-old girl with pilocytic astrocytoma (WHO grade I) in the left basal ganglia region. A, Axial ADC map reveals high ADC values. B and C, Spectrum analysis from the enhancing tumor area shows slightly increased Cho/Cr ratio, and slightly decreased NAA/Cr and NAA/Cho ratios. D, Hematoxylin-eosin staining (400) of pilocytic astrocytoma demonstrates astrocytic cells with loose myxoid matrix and Rosenthal fibers.",yes
PMC4553915,Figure_2,oa_package/aa/18/PMC4553915.tar.gz,['LEC markers are expressed in neovascular hemi-RVO tissue specimen.'],"Fig. 2 LEC markers are expressed in neovascular hemi-RVO tissue specimen. , LYVE-1, PDPN, and nuclear transcription factor Prox-1 were used as markers for LECs. LYVE-1 immunoreactivity was observed (arrow head) ( , middle panel); immunoreactivity of PDPN was not found in any vascular structures, only in the extravascular structures ( , right panel), and corresponding pan-endothelial marker CD31 staining is also shown (arrow head) ( , left panel). Prox-1 positivity was observed in vessel-lining cells and also in circulating bone marrow-derived cells (arrow heads) ( ). Immunostaining of CD31, PDPN, and Prox-1 of the skin sample was used as control. Arrows indicate a blood vessel in the corresponding sections, and arrow heads indicate a lymphatic vessel showing positivity for PDPN and Prox-1. Coimmunostaining of Prox-1 and Ki67 shows active proliferation of Prox-1-positive lumen-lining cells.",yes
PMC6667607,Figure_15,oa_package/74/a4/PMC6667607.tar.gz,[],"Fig. 15 Interphalangeal joint sesamoid. A 33-year-old man referred for flat foot. Incidental finding of an interphalangeal joint sesamoid. Sagittal CT reconstruction better demonstrates its position with respect to the interphalangeal joint (black arrow). A potential complication arises if there is a phalangeal dislocation and the ossicle displaces into the joint space, blocking reduction",yes
PMC7813340,Figure_5,oa_package/dd/e5/PMC7813340.tar.gz,"['Diffuse GGO and interlobular septal thickening were noted, predominantly involving peripheral zones of bilateral lungs, more so in the lower lobes consistent with COVID-19 Reporting and Data System (CORADS) 6 (figure 5).', 'Coronal lung window image of a COVID-19-positive patient showing patchy areas of peripherally distributed ground-glass opacities in bilateral lungs, more so in the lower lobes consistent with COVID-19 Reporting and Data System (CORADS) 6.']","Figure 5 Coronal lung window image of a COVID-19-positive patient showing patchy areas of peripherally distributed ground-glass opacities in bilateral lungs, more so in the lower lobes consistent with COVID-19 Reporting and Data System (CORADS) 6.",yes
PMC3101212,Figure_1,oa_package/50/e7/PMC3101212.tar.gz,"['ResultsMuscle regeneration is deficient in tissue affected by hemorrhageHistological observations showed that all treatments, except PBS, induced evident myonecrosis in the injected gastrocnemius one day after injection ().', 'In the case of muscle injected with Mtx, by 7 days, regenerating fibers were distributed uniformly in the tissue ().', 'In contrast, muscle injected with venom or BaP1 showed areas of regenerating fibers intermixed with areas of remnants of necrotic cells, which had very low numbers of phagocytes, and areas of fibrosis ().', 'Therefore, the histological pattern at 7 days in muscle injected with venom was characterized by heterogeneity, whereas in the case of tissue affected by Mtx, a homogeneous pattern predominated ().', 'Fibrosis was corroborated 7 days after injection, by staining with Sirius Red, in the endomysium and perimysium in muscle injected with venom or BaP1 (), whereas the extent of collagen deposition was less evident in Mtx-injected muscle, being nevertheless higher than in PBS-injected muscle.', 'By 28 days, tissue injected with Mtx presented a pattern of successful regeneration, with abundant regenerating fibers of similar size having centrally-located nuclei, and with little fibrosis, albeit with an increment in the interstitial area as compared with PBS-injected muscle ().', 'In contrast, in the case of muscle injected with venom or BaP1, which induced hemorrhage, regenerating fibers of small size were observed, and were intermixed with areas of fibrosis and, in some cases, with areas in which the remnants of necrotic fibers had apparently become calcified ().', 'g001Light micrographs of sections of mouse skeletal muscle at 1, 7 and 28 days after the injection, in the gastrocnemius muscle, of phosphate-buffered saline solution (PBS), B.']",10.1371/journal.pone.0019834.g001,yes
PMC9330607,Figure_5,oa_package/e5/c5/PMC9330607.tar.gz,"['Visualization shows examples of heatmaps generated by Grad-CAM and Grad-CAM++ using the network activations of GLS_net on images from the IF dataset that contain GLS.', 'Grad-CAM heatmaps in a,b show activations around some of the GLS lesions.', 'However, in c the main activation corresponds to a bright region above the leaf edge, with a weak activation around the lesion on the leaf.', 'However, when applied to diseased maize leaf images, Grad-CAM++ heatmaps tended to activate in regions of high contrast on the images, such as the edges of leaves as can be seen in c.', 'Heatmaps from Grad-CAM and Grad-CAM++ software, which are designed to illustrate image regions detected as GLS positive by a CNN, such as GLS_net, are shown here.']","Figure 5 Heatmaps from Grad-CAM and Grad-CAM++ software, which are designed to illustrate image regions detected as GLS positive by a CNN, such as GLS_net, are shown here. Panels ( ) show three GLS positive representative images from the IF dataset. Each panel shows (from left to right) the input image that was scored as GLS positive by GLS_net, the Grad-CAM heatmap, and the Grad-CAM++ heatmap, respectively. Panel ( ) contains a colour scale to aid in interpretation, blue indicates no activation, while red indicates high levels of activation.",yes
PMC7402742,Figure_3,oa_package/d4/79/PMC7402742.tar.gz,"['Cells are arranged around thin-walled vascular spaces, with a staghorn appearance, [hematoxylin and eosin (H and E stain, original magnification 200] (A and B), confirming the hypervascularity and non-epithelial origin of the neoplasms, which exhibited a mild nuclear pleomorphism, low mitotic rate ( 5 mitoses/10 HPF), and few areas of necrosis.']","Figure 3 Cells are arranged around thin-walled vascular spaces, with a staghorn appearance, [hematoxylin and eosin (H and E stain, original magnification 200] (A and B), confirming the hypervascularity and non-epithelial origin of the neoplasms, which exhibited a mild nuclear pleomorphism, low mitotic rate (<5 mitoses/10 HPF), and few areas of necrosis. Immunohistochemical analysis was positive for CD34 (C) and vimentin (D), but negative for epithelial membrane antigen (E) and glial fibrillary acidic protein (GFAP) (F). H&E = hematoxylin and eosin.",yes
PMC8576648,Figure_3,oa_package/be/af/PMC8576648.tar.gz,"[' displays the anatomy of the chest after surgical resection of the tumor and affected chest wall.', 'The anatomy of the chest after surgical resection of the tumor and the affected chest wall.']",Figure 3 The anatomy of the chest after surgical resection of the tumor and the affected chest wall.,yes
PMC10486883,Figure_10,oa_package/cc/a2/PMC10486883.tar.gz,[],Figure 10 The 6-month postoperative sagittal and coronal MRI scans showing no signs of relapse on the right TMJ.,yes
PMC10831814,Figure_5,oa_package/af/ba/PMC10831814.tar.gz,"['We again examined LPAC activity in layer 4 (A) and identified increased firing in global Fmr1 mutants as compared to both cON-wild-type and cON-PC cohorts (A).', '.']","Figure 5. Cerebellar reduces LPAC hyperexcitability (A) Schematic of electrode placement in LPAC layer 4 and representative traces from cON-wild-type, cON-null, and cON-PC mice. (B and C) Multi- (B) and single-unit (C) analysis of anesthetized recording of the LPAC. The waveforms on the left (C) reflect the first 100 assigned to a single unit (gray) with a superimposed average waveform (red). Two-way ANOVA, Bonferroni post hoc analysis. *p < 0.05 and **p < 0.01. Data are reported as mean SEM. n 10 for all groups. Complete p values and animal numbers can be found in .",yes
PMC9577059,Figure_3,oa_package/61/fc/PMC9577059.tar.gz,"['34To tackle this, we propose adding three extra feature channels (analogous to image size) to both the input (M1) and output (M2) of each generator, with the input zero-padded by three extra channels ().', 'Extra channels for meta-learning.', 'EvaluationOur objective is to enable and improve the applicability of an already existing segmentation model S to arbitrary unknown stains.']","Fig. 3 Extra channels for meta-learning. The proposed incorporation of extra channels into the CycleGAN is exemplified for one of the two translational directions. The input is zero-padded by three channels and the output now includes the translation as well as three additional channels that can be used to implicitly learn meta-information. Both are then translated back for input reconstruction (ignoring in turn its extra channels ). Here, represents the concatenation operation.",yes
PMC5127798,Figure_4,oa_package/38/71/PMC5127798.tar.gz,['Clinical assessment of colitis and macroscopic evidence of inflammation in DSS-treated mice.'],"Figure 4 . Body weight changes were recorded daily in males and females . Lack of AM results in a significant weight loss. KOF experience a higher percentage of weight loss. Colitis symptoms were scored on a 0-12 point scale in males and females . KO animals exhibited severe colitis symptoms reaching higher scores than their WT counterparts. DSS treatment caused local inflammation of the colon in both males and females . There is a significant increase in the weight/length ratio of the colon in all DSS-treated mice when compared with their respective controls. However, this inflammatory response is more pronounced in deficient mice. Data are shown as mean SEM. ANOVA + Tukey's MCT; < 0.05; < 0.01; < 0.001; < 0.0001. Same abbreviations as in Figure .",yes
PMC9400269,Figure_1,oa_package/17/16/PMC9400269.tar.gz,"[' 1a, levels of plasma total apoE were the highest in APOE 2/ 3 subjects and the lowest in individuals with the 4/ 4 genotype.', ' 1a).', ' 1b).', 'Levels of plasma apoE in subjects with different APOE genotypes.', 'Group comparisons were done using the Kruskal Wallis test followed by Dunn s test (a, b) before/after Bonferroni correction for multiple comparisons or ANOVA with Tukey HSD as post hoc test (c)Plasma total apoE levels and effects of sexWith female sex as a strong risk factor for AD [48, 49], we examined whether sex was associated with variations in plasma apoE levels.', ' 1c).', 'Supplementary .']","Fig. 1 Levels of plasma apoE in subjects with different genotypes. Plasma apoE levels as assessed in subjects grouped based on their genotype ( ), 4 status ( ), and in males and females with different genotype ( ). Data are shown as median (minimummaximum). Group comparisons were done using the KruskalWallis test followed by Dunns test ( , ) before/after Bonferroni correction for multiple comparisons or ANOVA with Tukey HSD as post hoc test ( )",yes
PMC6835383,Figure_1,oa_package/f3/7b/PMC6835383.tar.gz,"['Papillary muscle calcification was observed in three rats, two of them belonging to fish oil group and one of them to virgin olive oil group (A).', 'At valvular level, acellular material deposits rich in mucopolysaccharides were seen in almost every animal, but not accompanied by an inflammatory infiltrate (B).', 'One of them, within sunflower oil group, suffered from endomyocardial hyperplasia (C).', 'Associated with chronic progressive cardiomyopathy, vacuolar myocardial degeneration was also observed on a regular time basis (D).', '970Representative images of histopathological features found in the heart of 24-months-old rats.']","Figure 1 Representative images of histopathological features found in the heart of 24-months-old rats. ( ) Papillary muscle calcification of left ventricle (white arrow). H & E 20. ( ) Mucinous degeneration in the cardiac valves. H & E, 4. Insert 20. ( ) Endomiocardic hyperplasia. The proliferation of spindle-shaped cells. H & E, 4. Insert, 20. ( ) Vacuolar degeneration (fat) at intramyocardial level. H & E 20.",yes
PMC5995720,Figure_1,oa_package/59/52/PMC5995720.tar.gz,[],"Figure. The patients left leg on arrival. His left leg showed redness, swelling, and tenderness.",yes
PMC7418370,Figure_4,oa_package/e0/d3/PMC7418370.tar.gz,"[' 4a).', '4b).', '4b, white arrows).', '4c-d).', 'p53 oligomers detected in aged tau mouse models, but not in aged control mice.', '(n = 3 in technical duplicate)Furthermore, Western blot analysis of all four mouse models (', '4e) containing aged mice (C57Bl/6 age:17 months; htau age 13, 14, 19 months; P301L age 16 months; Tau KO age 16 months) demonstrated a significant increase in p53 monomer in P301L mice compared to all other mouse groups (', '4f).', '4f), suggesting that tau overexpression causes high molecular weight p53 to form.', '4g) and demonstrate significantly more MDM2 in the htau and P301L mice as compared to both C57Bl/6 and Tau KO control mice (', '4h).']","Fig. 4 p53 oligomers detected in aged tau mouse models, but not in aged control mice. Representative immunofluorescent confocal images of cortex of 14-month-old htau, 5-month-old P301L, and 9-month-old C57BL/6 mice. Brain tissue probed with anti-p53 (red) and anti-I11 (green; oligomer-specific) in merge panel. Magnified ROI from merged panel in A. (htau ROI 1 and 2) Peri-nuclear colocalization between p53 and I11 is shown with other oligomers in the vicinity. (P301L ROI 1 and 2) Peri-nuclear, circular colocalization between p53 and I11 is shown with other oligomers in the vicinity. (C57BL/6 ROI 1) No colocalization between p53 and I11 is detected in C57BL/6 mice. Keyence Microscope 60X magnification. Scale bar=50m. Graph depicting immunofluorescent intensity for I11 oligomer ( =2 in technical triplicate) and ( ) p53 intensities from htau and C57BL/6 mice ( =2 in technical triplicate). Keyence Microscope 60X magnification. Scale bar=50m. Western blot of aged C57BL/6, htau, P301L, and Tau KO mice probed with anti-p53. Densitometry of p53 bands shows a significant increase in p53 in htau mice at multiple bands compared to C57BL/6 and Tau KO mice ( =3). Western blot of same mice probed with anti-MDM2 (90 kD). Densitometry of MDM2 from G shows significant increase in MDM2 in htau compared to all other mouse models. P301L also show significantly more MDM2 compared to control and Tau KO mice. ( =3 in technical duplicate)",yes
PMC6667521,Figure_2,oa_package/40/96/PMC6667521.tar.gz,"[' 2).', '24-year-old man, history of ankle sprain and persisting pain in the lateral and posterior aspect of the ankle.', 'Injury to the synchondrosis is one of the causes of symptomatic os trigonumSymptoms of os trigonum syndrome may result from all the situations mentioned above and consist of chronic or recurrent pain with stiffness, soft tissue swelling and tenderness to palpation in the postero-lateral aspect [8].']","Fig. 2 24-year-old man, history of ankle sprain and persisting pain in the lateral and posterior aspect of the ankle. Sagittal proton density spectral attenuation inversion recovery (PD SPAIR) image. Note the hyperintense band of fluid between the os trigonum and the posterior aspect of the talus, in keeping with disruption of the synchondrosis (white arrow). Injury to the synchondrosis is one of the causes of symptomatic os trigonum",yes
PMC7738527,Figure_7,oa_package/c3/23/PMC7738527.tar.gz,"[' 7A,B).', ' 7A,B).', 'IL-2R -KO T cells exhibit differential proliferation and apoptosis.', 'While the primary site of hemolytic anemia is the spleen, we also performed staining and functional assessments on lymphocytes as disease in IL-2R -KO and IL-2-KO mice is systemic and lymph nodes are a site of T cell activation.']","Figure 7 IL-2R-KO T cells exhibit differential proliferation and apoptosis. Frequency of ( ) Ki67+or ( ) annexin V+19-day-old and late endpoint (d27+) WT and IL-2R-KO T cells from the spleen are shown. =39 mice per experimental group. Statistics: one-way ANOVA with BenjaminiHochberg FDR correction; *p<0.05, **p<0.01, ***p<0.001. Non-significant comparisons are not shown.",yes
PMC10733535,Figure_2,oa_package/31/62/PMC10733535.tar.gz,"['As injectable biomaterials, hydrogels have strong therapeutic applications and have gained widespread attention in the study of IVDD ().']","FIGURE 2 Normal intervertebral disc, herniation, and treatments ( ).",yes
PMC11376460,Figure_6,oa_package/eb/2e/PMC11376460.tar.gz,"['A summary of the molecular findings is shown in A.', '2]), whereas the number of cases is too small to draw a statistical conclusion (B).', ':Summary of the molecular findings in a multilayer circle diagram.', 'Characteristics of the pedHGG_H3-/IDH-wt Subgroup With GC PhenotypeIn the pedHGG_H3-/IDH-wt subgroup, 29 of the 32 patients (90.', 'pedHGG_A/B) (C), nor the presence of structural alterations of chromosome 6 were associated with survival.']","Figure 6: Summary of the molecular findings in a multilayer circle diagram. (A) In total, molecular data was available of 52 tumors. represents different tumor types according to WHO CNS 2021 in synopsis with methylation array data and exome sequencing. illustrates the DNA methylation-based subtypes according to the MNP12.5 classifier, which clustered to the corresponding WHO CNS types. In addition, the most frequent genetic alterations of the respective subtypes are given. In both circles the (sub-)types are colored as labeled in the given key. Hatched areas represent cases with inconclusive methylation profiling, but subtype allocation was possible through detection of disease-defining genetic alterations in exome sequencing. *adult-type diffuse gliomas, IDH-wild-type ( =2) including 1 case each of the subtypes GBM_MES_ATYP and GBM_RTK2. Nine cases were NEC in methylation analyses, and additionally, in 3 cases, no methylation data were available. represents the relative frequency of chromosome 6 rearrangements sorted by the respective methylome-based subclass. (B, C) KaplanMeier plots of the overall survival in months according to (B) the WHO CNS 2021 and to (C) the methylation-defined subclasses of the pedHGG_H3-/IDH-wt subgroup including pedHGG_A/B. Tumors of the adult-type, IDH-wild-type are not shown in (B).",yes
PMC5061513,Figure_7,oa_package/c6/89/PMC5061513.tar.gz,"['knowlesi (as schematized in ).', 'g007PkSBP1 delineates P.']",10.1371/journal.pone.0164272.g007,yes
PMC6125269,Figure_3,oa_package/be/9f/PMC6125269.tar.gz,"['Second, endoscopic retrieval of the remaining fractured fragments () was done the next day.', 'Endoscopic view of distal fracture fragment (arrow A), intact left PTBD catheter (Arrow B), and temporarily inserted 4 Fr right biliary catheter (arrow C).']","Fig. 3 Endoscopic view of distal fracture fragment (arrow A), intact left PTBD catheter (Arrow B), and temporarily inserted 4 Fr right biliary catheter (arrow C).",yes
PMC5404121,Figure_3,oa_package/ff/cc/PMC5404121.tar.gz,"['Computed tomography (CT) examination, left lateral projection: the 6 segments of the ascending aorta evaluated in the patient in whom preoperative CT was performed are marked']","Fig. 3 Computed tomography (CT) examination, left lateral projection: the 6 segments of the ascending aorta evaluated in the patient in whom preoperative CT was performed are marked",yes
PMC7599077,Figure_9,oa_package/eb/d3/PMC7599077.tar.gz,[],"Figure4 TDP-43 Causes mtDNA Release to the Cytoplasm via the mPTP (A) Plasmids encoding TDP-43 (WT or Q331K) were transiently overexpressed in MEFs that are genetically deficient for Bax and Bak. Expression of and was measured by qPCR after 72 h. (B) Human iPSC-derived motor neurons from healthy controls and ALS patients carrying mutations in TDP-43 (G298S, M337V, and A382T) were treated with mitoSOX red to quantify mitochondrial ROS 2weeks after terminal differentiation and then subjected to fluorescence-activated cell sorting (FACS) analysis (MFI, mean fluorescence intensity). (C) OMX-SR microscopy reveals that TDP-43-induced (FLAG-tagged, red) relocation of DNA (anti-DNA, green) from mitochondria (TIM44, blue; TOM20, cyan) into the cytoplasm was reduced significantly by inhibition of the mPTP (CsA, 12.5M) in TDP-43 mutant (Q331K)-overexpressing MEFs (scale bars, 5m). DMSO was used as a solvent control. Overview images are maximum-intensity projections, and magnified images are 3D surface reconstructions using Imaris software (bottom right) (scale bars, 0.5m). See also . (D) Spatial quantification by Imaris software for the percentage of DNA outside of mitochondria (TIM44); 3040 cells per group. (E and F) Inhibition of the mPTP (CsA, 12.5M) in HEK293T cells prevents mtDNA cytosolic accumulation (cytosolic/total lysis, percent) and (F) prevents gene expression relative to , as induced by TDP-43 transient overexpression (WT, A315T, or Q331K). (G and H) CRISPR-mediated genetic deletion of the mPTP component also abolished mtDNA release into the cytoplasm and (H) downstream gene expression. Data are mean SEM, pooled from 3 independent experiments ([A], [B], and [D][H]) or representative of 3 independent experiments (C). The p values were calculated using unpaired t test in (B) and (D), two-way ANOVA to control in (A) and (E)(H). p< 0.05, p< 0.01, p< 0.001, p< 0.0001. See also .",yes
PMC8406332,Figure_4,oa_package/b6/a3/PMC8406332.tar.gz,"['We found that CCR2 was expressed on the surface of CD45hiCD11b+Ly6C+ IM (', ' S4) but not microglia (CD45lowCD11b+) or T cells (CD45hiCD3+) (', 'CCR2 gene deletion abolished IM accumulation by 95% in the brain of CM mice at 21 dpi (', 'Systemically, infected CCR2 / mice showed a decreased frequency of splenic CD11b+Ly6C+ monocytes but not CD4+ T cells and CD8+ T cells in the spleens compared to those of WT mice (', ' 4D and E), consistent with findings observed in our studies of cryptococcal lung infection, in which CCR2 was shown to mediate mainly Ly-6Chigh monocyte egress from the bone marrow (22, 23).', 'FIG 4CCR2 pathway is essential for the accumulation of inflammatory monocytes (IM) in the C.', 'After 24 h posttransfer, we found that CCR2-deficient monocytes migrated into the brain of the recipient mice with 50% efficiency relative to that of the WT monocytes (']","FIG4 CCR2 pathway is essential for the accumulation of inflammatory monocytes (IM) in the infected brain. Brain leukocytes from WT and CCR2 mice were isolated and analyzed. (A and B) CCR2 is mainly expressed by IM but not microglia and T cells in the brain during CM. The gray area in the flow plot and dotted line in the bar graph represent the signal from isotype control. (C) Accumulation of Ly6C CD11b IM in the infected brain at days 0, 14, 21, and 35 was evaluated. IM were absent until 14dpi and showed peak accumulation on 21dpi in WT mice. Note there was a profound reduction in the total number of IM in the brain of CCR2 mice compared to that in the WT mice. (D and E) The frequencies of CD11b Ly6C monocytes as well as CD4 T cells and CD8 T cells in spleens of WT and CCR2 mice at 21dpi. (F) Sorted monocytes from WT (CD45.1) or CCR2 (CD45.2) mice were mixed at a 1:1 ratio and confirmed by flow cytometry (left panel) and then injected into WT (CD45.1 CD45.2) recipients at 20dpi. Frequency and the total numbers of WT and CCR2 donor monocytes in the brain of recipient mice were analyzed by flow cytometry at 24h posttransfer (right two panels). Representative flow cytometric dot plots are shown after CD11b Ly6C IM gating. Numerical data or bar graphs showed mean SEM from an experiment representative of two to four independent experiments ( >3). *, < 0.05; **, < 0.01; ***, < 0.001.",yes
PMC5265201,Figure_7,oa_package/a0/51/PMC5265201.tar.gz,"[' 7a and b).', ' 7a) [22].', ' 7b) [23].', 'Classic imaging signs of bronchiectasis.', 'b Finger-in-glove sign describes the presence of mucous or fluid impaction within a dilated bronchus (dashed arrow) appearing as a radiopaque finger in the glove of the bronchus\nThere are three main forms of bronchiectasis, characterised by morphological pattern: cylindrical, varicose, and cystic types (']",Fig. 7 Classic imaging signs of bronchiectasis. Signet ring sign is a CT finding referring to a markedly enlarged bronchus (*) mimicking a ring and its adjacent normal accompanying pulmonary artery representing the signet emblem of the ring ( ). Finger-in-glove sign describes the presence of mucous or fluid impaction within a dilated bronchus ( ) appearing as a radiopaque finger in the glove of the bronchus,yes
PMC9742706,Figure_1,oa_package/56/7f/PMC9742706.tar.gz,"[' 1 shows an overview of the training method.', ' 1.', '1177_15330338221142674-fig1"" position=""float""/>We performed slide tiling by extracting square tiles from tissue regions of the WSIs.', 'The models were applied in a sliding window fashion with an input tile size and stride of 224 224 pixels ( 1).']","Figure1. Schematic diagrams of training methods. (A) The simple summary of training method using transfer learning and weakly supervised learning for this study. During training (B), we iteratively alternated between inference and training. During the inference step, the model weights were frozen and the model was used to select tiles with the highest probability after applying it on the entire tissue regions of each hole-slide image (WSI). The top tiles with the highest probabilities were then selected from each WSI and placed into a queue. During training, the selected tiles from multiple WSIs formed a training batch and were used to train the model.",yes
PMC4219597,Figure_2,oa_package/ed/b7/PMC4219597.tar.gz,['Midline dorsal penile shaft incision.'],Figure 2 The operation started with a midline dorsal penile shaft incision to access and remove the scarred tissue between dartos and Bucks fascia dorsally.,yes
PMC9495444,Figure_6,oa_package/d2/62/PMC9495444.tar.gz,"[' presents the histopathological findings of various tissues in the ASFV-infected pigs in this study.', 'Histopathological findings in pigs experimentally infected with African swine fever virus.']","Figure 6 Histopathological findings in pigs experimentally infected with African swine fever virus. Spleen [pig #54: died at 3 dpv [(Group I)]: Severe lymphoid depletion with the presence of pyknosis and karyorrhexis in the lymphoid follicle (Lf); Spleen [pig #60: died at 6 dpv (Group II)]: Mild lymphocytic depletion in germinal center (Gc), The marginal zone (Mz) infiltrated by erythrocytes, severe and diffuse engorgement in the red pulp (Rp); Spleen [pig #48: died at 4 dpv (Group I)]: Necrosis of lymphocytes (arrow) within the lymphoid follicle (Lf); Submandibular lymph nodes (pig #54): Severe congestion (arrows); Submandibular lymph nodes (pig #60): Focal necrosis (arrow); Liver (pig #54): Neutrophils (arrows) in sinusoids. Liver (pig #60): Multifocal congestions (dash circle) and the cytoplasmic vacuoles (arrows); Liver (pig #43: died at 7 dpv [Group II]): Vacuolar degeneration with necrotic hepatocytes (arrow); Lungs (pig #54): Pulmonary hemorrhages with edema (arrows); Lungs (pig #60): Moderate pulmonary hemorrhages with edema fluids (arrows) and interstitial pneumonia; Kidney (pig #48): Congestion (arrows) and interstitial edema (arrowhead) in the renal medulla.; Kidney (pig #60): Multifocal hemorrhage (dash circles) and mononuclear cell infiltration in the renal interstitium (arrows). Hematoxylin and eosin stain (400 magnification; scale bar, 50 m). dpv, days post-viremia; Gc, germinal center; Lf, lymphoid follicle; Mz, marginal zone; Rp, red pulp.",yes
PMC3564530,Figure_4,oa_package/26/7b/PMC3564530.tar.gz,"['Membrane-associated BGIN was incrementally boosted with MG132 treatment in both stably expressing (A) and transiently transfected HeLa cells (Supplemental S4A), indicating that BGIN distribution to membranes can be influenced by poly-Ub accumulation.', 'Moreover, Rac1 was exclusively distributed to cytosolic/membrane fractions and exhibited little change with MG132 treatment (A, adjacent line graphs).', 'We then investigated the effects of uncoupling poly-Ub/BGIN interactions on BGIN subcellular distribution.', 'We subcloned three CIN exon 2 alleles (mut159, 167, and 168) into full-length BGIN and confirmed that the allelic variants lacked the capacity to bind poly-Ub in the absence or presence of MG132 (B).', 'GST-tagged wild-type and mutant BGIN isoforms were expressed in HeLa cells, and their abundances in cytosolic, membrane, and insoluble fractions were determined under steady-state and MG132-treated conditions (C).', 'Of interest, non-poly-Ub binding BGIN variants largely failed to partition to both detergent-soluble membrane and insoluble fractions in comparison to wild-type BGIN under both steady-state and MG132-treated conditions (C), where little difference was seen in cytosolic abundance of wild-type and mutant BGIN alleles.', 'HeLa cells are multipolar and typically spread with numerous F-actin rich apexes (D).', 'Whereas expression of the mut159 allele also results in a multipolar phenotype, expression of wild-type BGIN produces cells that are often elongated and primarily bipolar (D).', 'Wild-type BGIN attenuated cell spreading after 45 min plating on fibronectin, whereas the mut159 allele had little effect on spreading (E).', 'It is striking that many of the mut159 cells protruded with a thick lamellipodial layer similar to cells transfected with the active Rac1 Q61L allele (E).']","FIGURE 4: Interactions between BGIN and poly-ubiquitin promote BGIN partitioning to membrane fractions. (A) Poly-ubiquitin accumulation triggers BGIN distribution to detergent-soluble membrane fractions. Stably expressing BGIN-GFP HeLa cells were treated with MG132, and lysates were separated into cytosolic and detergent-soluble/insoluble membrane fractions. Quantification of Rac1 in cytosolic and detergent-soluble membrane fractions are presented as mean SEM from three independent experiments, where the peak intensity is set to 1.0 (** < 0.001). (B) CIN exon 2 mut159, 167, and 168 alleles were subcloned into full-length GST-BGIN constructs, expressed in HeLa cells, and assayed for poly-Ub interactions under steady-state and presence of MG132 (5 M, 5 h) by GST pull-down assay. (C) GST-tagged wild-type BGIN and two poly-Ub refractory mutants (mut159 and mut167) from B were expressed in untreated or MG132-treated (5 M, 5 h) HeLa cells and separated into the subcellular fractions indicated. Equal protein quantities were immunoblotted from each fraction and quantified as a ratio in comparison to the wild-type BGIN (set to 1.0). Values of mean SEM from three independent experiments are presented (** < 0.003). (D) Morphology of cells stably expressing wild-type or mut159 BGIN-GFP. A total of 10 cells seeded on 15-mm coverslips were stained for F-actin and scored for a multipolar morphology (more than two apexes) or an elongated/bipolar phenotype (primarily two apexes). Infrequent appearance of rounded cells were excluded from scoring. Images shown are inverted phalloidin-stained images. The graph displayed are cumulative percentages of multipolar/bipolar cells from four experiments (mean SD, ** < 0.0005). (E) Cell spreading on fibronectin. HeLa cells transiently expressing GFP alone or constitutively active GFP-Rac1 Q61L or stably expressing wild-type or mut159 BGIN-GFP were seeded onto 0.0001% fibronectin coverslips for 45 min and costained for actin and paxillin. Total cell area was measured under each condition from three independent experiments (mean SD shown in the adjacent graph, ** < 3 10 ).",yes
PMC9644442,Figure_3,oa_package/76/19/PMC9644442.tar.gz,['Ultrasound image using a low frequency curvilinear probe of the aortic aneurysm construct in gelatin mixture in transverse (a) and longitudinal (b) orientation with measurements.'],Figure 3 Ultrasound image using a lowfrequency curvilinear probe of the aortic aneurysm construct in gelatin mixture in transverse (a) and longitudinal (b) orientation with measurements. [Colour figure can be viewed at ],yes
PMC10507329,Figure_2,oa_package/ce/65/PMC10507329.tar.gz,[],"Figure2 Glucose metabolism is restored after ApN treatment in hippocampal slices Hippocampal slices were obtained from WT or APP/PSN1 mice treated with either ApN or resistin and the uptake of glucose, glycolytic flux, ATP/ADP ratio and pentose phosphate flux (PPP) were measured. Means SEM are shown (n = 4-5 mice per condition in 3 technical replicates). Statistical significance was determined by either one-way ANOVA or two-way ANOVA , followed by Bonferronis multiple comparison posthoc test. *p < 0.05, **p < 0.01 and ***p < 0.001, compared with control group; p < 0.05 and p < 0.01, p < 0.001 compared with specified group.",yes
PMC9085623,Figure_2,oa_package/ee/30/PMC9085623.tar.gz,"['The type of ASSD in these cases included tinea corporis (fungal infection) (A), intertriginous dermatitis and eczema (B), cutaneous keratosis (C), and verrucous hyperplasia and ulceration (D).']",FIGURE 2 Representative clinical lesion manifestations and patient distribution of ASSD. Stump tinea corporis with fungal infection of (left) and (right). Intertriginous dermatitis (left) and eczema (right). Cutaneous keratosis. Verrucous hyperplasia (left) and skin ulceration (right).,yes
PMC4849319,Figure_1,oa_package/3e/7a/PMC4849319.tar.gz,"['NCCT head showed infarct of bilateral cerebellar hemispheres and the brainstem [].', 'Bilateral cerebellar hemishpheric and brainstem infarctionFractured right foramen transversarium, pedicle, and lamina of C6 vertebra due to penetrating stab injuryDiscussionIn penetrating trauma, the most commonly injured structures in the neck are the vessels, followed by the spinal cord, the aerodigestive tracts, and nerves.']",Figure 1 Bilateral cerebellar hemishpheric and brainstem infarction,yes
PMC11381350,Figure_5,oa_package/00/07/PMC11381350.tar.gz,"['.', 'DiscussionThe detrimental effects of reduced SMCHD1 levels in mature muscle myofibers have been shown to be at the heart of the FSHD disease pathology in FSHD2 patients.']","Figure 4. Binding regions of SMCHD1 in the human myoblast genome. ( ) The enrichment of H3K9me3, H3K4me1,H3K4me3, H3K27me3 and H3K27ac around SMCHD1 peak centers at SMCHD1 co-activated genes. ( ) Percentage of direct targets-associated peaks that were located at promoters and enhancers. score and -value were calculated by permutation test using regioneR. ( ) UCSC genome browser tracts showing ENCODE regulatory elements, GeneHancer annotated enhancer/promoter regions () and their regulatory interactions, as well as ChIP-Seq (SMCHD1), CUT&Tag (H3K4me3 and H3K27ac) and RNA-Seq reads (control: proliferating myoblasts expressing non-silencing scrambled shRNA, shSMCHD1: proliferating myoblasts expressing shRNA targeting ) near the gene.",yes
PMC10375206,Figure_3,oa_package/21/f7/PMC10375206.tar.gz,['(a) Photograph of the eyelid after incisional biopsy.'],Figure 3 Photograph of the eyelid after incisional biopsy. Tarsal conjunctival photography after the incisional biopsy.,yes
PMC7567048,Figure_1,oa_package/d6/da/PMC7567048.tar.gz,"['The plain radiograph was normal, () and MRI study was unremarkable.', 'Anteroposterior pelvis radiograph of the reported case.', '']",Fig. 1 Anteroposterior pelvis radiograph of the reported case.,yes
PMC3414438,Figure_4,oa_package/76/bc/PMC3414438.tar.gz,"['As shown in A-C, the round apoptosis area could be readily distinguished after 30 min.', 'Apoptotic cells showed green fluorescence (YO-PRO -1; D), and dead cells showed primarily red fluorescence (PI; E,J).', 'Blue-fluorescent Hoechst 33342 brightly stained the condensed chromatin of the apoptotic cells and more dimly stained the normal chromatin of the live cells (F,K).', 'The cell borders and the small, rounded humps mentioned previously could be observed clearly (G).', 'The nuclei of the apoptotic cells moved aside, and round caves were left in the original sites (H, rectangle).', 'After 20 h, the nuclei were invisible under an optical microscope, and there were only round caves in the irradiation area (I, rectangle).', 'However, fluorescent staining showed that all of the LECs were dead, and the nuclei lay adjacent to the round caves (L, rectangle).', 'Imaging of apoptosis of the LECs.']","Figure 4 Imaging of apoptosis of the LECs. The round apoptosis area could be readily distinguished after 30 min ( ). Apoptotic cells showed green fluorescence (YO-PRO -1; ), and dead cells showed primarily red fluorescence (PI; , ). Blue-fluorescent Hoechst 33342 stained the condensed chromatin of apoptotic cells and the normal chromatin of live cells ( , ). : A merging of , , and . : A merging of and . After 30 min, the cell borders and small, rounded humps were clearly observed ( ). After five h, the nuclei of the apoptotic cells moved aside, and round caves were left in the original sites ( ). After 20 h, the nuclei were invisible under an optical microscope, and there were only round caves in the irradiation area ( ). Fluorescent staining showed that all of the cells were dead, and the nuclei lay adjacent to the round caves ( ). : A merging of , , and . High-magnification images of the apoptotic cells are shown in the rectangles. Scale bars: =50 m.",yes
PMC8773081,Figure_1,oa_package/ed/0e/PMC8773081.tar.gz,"['Differences were observed between three categories of disease severity (control subjects, severe COVID-19 patients, critical COVID-19 patients) for activin A, activin B, FLRG, and PAI-1 () (P 0.']","FIG 1 Activin A, activin B, FLRG, and PAI-1 levels versus disease severity in COVID-19 patients prior to dosing and in non-COVID-19 controls. Activin A (A), activin B (B), FLRG (C), and PAI-1 (D) for control subjects, severe COVID-19 patients, and critical COVID-19 patients. Number of patients tested in each group (n) is indicated under each respective plot. Median values for each group are shown above each respective plot. Significant follow-up Dunn pairwise comparisons are shown above each plot; ***, < 0.001, ****, < 0.0001.",yes
PMC11123536,Figure_1,oa_package/39/10/PMC11123536.tar.gz,"['The distal small bowel and colon were divided into 11 different segments (), and the presence of increased mesenteric vascular appearance (comb sign) and contrast loading of the affected mucosa and serosa were noted (yes or no).', '.', '"">0 (0)#Segments divided as denoted in .']",Figure 1. The different segments of the distal small bowel and the colon.,yes
PMC3169809,Figure_4,oa_package/ba/ee/PMC3169809.tar.gz,"['Paul, MN, US) (s 4, 5, and 6).', '003""/>Frontal view of the left and right sides (posttreatment).']",Figure 4 Frontal view of the left and right sides (posttreatment).,yes
PMC3726683,Figure_2,oa_package/75/54/PMC3726683.tar.gz,"['As shown in A and 2B, ELAVL1/HuR protein physically interacts with the SQSTM1/p62 probe, leading to the formation of a stable protein-RNA probe complex.', 'g002\nSQSTM1/p62 transcript as a new target of the RNA-binding ELAVL1/HuR protein.', 'It was found that the MG-132-induced up-regulation of SQSTM1/p62 mRNA in the cytoplasm of ARPE-19 cells (C) was accompanied by a parallel increase of the binding between ELAVL1/HuR protein and SQSTM1/p62 mRNA in the same cellular fraction (']",10.1371/journal.pone.0069563.g002,yes
PMC10691730,Figure_9,oa_package/10/31/PMC10691730.tar.gz,"['mansoni adult worms recovered from infected mice that had been treated with the anti-SmI-CLA-W conjugated nanomicelles (SGIVb) showed structural abnormalities compared to the untreated ().', 'Some worms showed extensive erosion of the tegument, vacuolization in syncytia and disruption of the subtegumental ultrastructure architecture (A and 9B).', 'Extensive lysis of the muscular layers, whorled myelin figures and swollen mitochondria were observed (C).', 'The subtegumental cytons revealed vacuolization of cytoplasm, irregularity in the nuclear membrane and swollen mitochondria (D).', 'The gastrodermis showed oedema and vacuolization in syncytial layer with decreased cytoplasmic extensions (E).', 'The ultrastructure of mature vitelline cell revealed vacuolization and fusion of vitelline droplets (F and 9G).', 'g009TEM of S.']",10.1371/journal.pntd.0011776.g009,yes
PMC5633293,Figure_1,oa_package/19/21/PMC5633293.tar.gz,"['In intraoperative, we found scleral necrosis with small abscess (A and B) at inferotemporal area.', 'Xanthogranulomatous inflammationPathol Res Pract18343954027KossardSWinkelmannRKNecrobiotic xanthogranuloma with paraproteinemiaJ Am Acad Dermatol19803325727074516938RoseGEPatelBCGarnerAWrightJEOrbital xanthogranuloma in adultsBr J Ophthamol1991756806849Sivak-CallcottJARootmanJRasmussenSLAdult xanthogranulomatous disease of the orbit and ocular adnexa: new immunohistochemical findings and clinical reviewBr J Ophthalmol20069056026081662209110GuoJWangJAdult orbital xanthogranulomatous disease: review of the literatureArch Pathol Lab Med200913312199419971996125911RaynerSADuncombeASKeefeMTheakerJMannersRMNecrobiotic xanthogranuloma occurring in an eyelid scarOrbit20082731911941856982712PeymanAWalshNGreenPDoreyMWSeamoneCPasternakSNecrobiotic xanthogranuloma associated with necrotizing scleritisAm J Dermatopathol20123466446472281432013LavricAAgrawalRPavesioCA case of necrobiotic xanthogranuloma presenting as bilateral posterior scleritisJ Immunol Inflamm20161314WilhelmusKRYenMTRiceLFontRLNecrobiotic xanthogranuloma with posterior scleritisArch Ophthalmol2006124515MohseninASinardJHuangJJNecrobiotic xanthogranuloma and chronic lymphocytic leukemia of the conjunctiva masquerading as scleritis and uveitisClin Ophthalmol20126204520472327188416UgurluSBartleyGBGibsonLENecrobiotic xanthogranuloma: long-term outcome of ocular and systemic involvementAm J Ophthalmol200012956516571084405917AlkatanHMAl-AbdullahAEAOrbital necrobiotic xanthogranuloma: a case reportInt J Pathol Clin Res20151218HoffmanGSKerrGSLeavittRYWegener s granulomatosis: an analysis of 158 patientsAnn Intern Med19921166488498173924019IsmailovaDSNovikovPIGrushaYOAbramovaYVBulanovNMMakarovEAThe frequency of ophthalmologic manifestations of granulomatosis with polyangiitis (Wegener s) and their relationship to systemic diseasesTer Arkh2017895697320UrseaRNussenblattRBRonaldRBWegener s granulomatosis, a patient education monograph prepared for the American Uveitis Society2200321PakrouNSelvaDLeibovitchIWegener s granulomatosis: ophthalmic manifestations and managementSemin Arthritis Rheum20063552842921661615122AhmedMNiffeneggerJHJakobiecFADiagnosis of limited ophthalmic Wegener granulomatosis: distinctive pathologic features with ANCA test confirmationInt Ophthalmol20082835461758980723IsaHLightmanSLuthertPJRoseGEVerityDHTaylorSRHistopathological features predictive of a clinical diagnosis of ophthalmic granulomatosis with polyangiitis (GPA)Int J Clin Exp Pathol2012576846892297766524MullerKLinJHOrbital granulomatosis with polyangiitis (Wegener granulomatosis): clinical and pathologic findingsArch Pathol Lab Med20141388111011142507630225KalinaPHLieJTCampbellRJGarrityJADiagnostic value and limitations of orbital biopsy in Wegener s granulomatosisOphthalmology19929911201241741123(A) and (B) show generalized diffuse scleritis with scleral abscess inferotemporal area.', 'Turbid yellowish vitreous fluid from diagnostic vitrectomy.']",Figure 1 ( ) and ( ) show generalized diffuse scleritis with scleral abscess inferotemporal area.,yes
PMC10632943,Figure_2,oa_package/55/bd/PMC10632943.tar.gz,[' Necrotic tissue shows hyphae (HE stain X40).'],Figure 2 Necrotic tissue shows hyphae (HE stain X40).,yes
PMC11577678,Figure_1,oa_package/79/be/PMC11577678.tar.gz,"[' 1A.', ' 1B.', '', 'Data expressed as the means SD (n = 8)The body weights were significantly lower in the CIA group than in the Con group after 5 weeks (P 0.', ' 1C).', ' 1D.', ' 1E).']","Fig.1 The effect of HFD on arthritis response in CIA rat. ( ) Procedure of the experiment. ( ) Representative images of ankle joints within individual groups. ( ) The weight of rats (g). ( ) AI of rats. ( ) The serum concentration of TC and TG as determined by ELISA. <0.05, <0.01 . CON group; <0.05, <0.01 vs CIA group. Data expressed as the meansSD (n=8)",yes
PMC6264593,Figure_7,oa_package/18/4a/PMC6264593.tar.gz,"[' 7), and in the thalamus ( 83%, p = 0.', '001All animals were treated with the same dose (10 mg/kg) of the indicated antibodiesNS not significant, SEM standard error of the meanSAR228810, the humanized version of SAR255952, has consistent activity in immunotolerized APPSL mice.', 'Horizontal black lines and error bars denote mean SEM dataIn the hippocampus, immunotolerization did not affect cystatin F mRNA levels and, compared with control IgG, treatment by SAR255952 or SAR228810 reduced cystatin F mRNA levels by 66% and 70% (p 0.', ' 7c, Table 4), confirming the decrease in plaque-associated inflammation.']","Fig. 7 SAR228810, the humanized version of SAR255952, has consistent activity in immunotolerized APPSL mice. Animals were depleted in immunocompetent cells by treatment with a monoclonal anti-CD4+ antibody before undergoing a weekly treatment with 10mg/kgi.p. of the indicated amyloid (A) antibodies (labeled + in the micrographs). Representative whole-scanned A-immunostained sagittal brain tissue section illustrating the decrease in the extracellular A peptide deposition process in cortical, hippocampal, and thalamic brain areas in representative immunotolerized mice chronically treated either with SAR255952 or SAR228810, in respect to immunotolerized or not Ctrl-igG1-treated mice. Mean values of the total surface occupied by A immunostaining measured in the cortex over eight sections per animal. The immunodepletion has no significant effect on the cortical A peptide deposition process in Ctrl-IgG1-treated mice. MeanSEM real-time PCR amplification readings for the brain inflammation marker cystatin F expression in the hippocampus. Statistical analysis denotes similar significant effects of both SAR228810 and SAR255952. Of note, there was no major effect of the immunotolerization on A peptide deposition in the brain of APPSL mice. ** <0.01, *** <0.001, versus Ctrl-IgG1-treated controls. Horizontal black lines and error bars denote meanSEM data",yes
PMC11452557,Figure_19,oa_package/0e/c4/PMC11452557.tar.gz,[],Fig. 19 Creatine kinase-MB (CK-MB).,yes
PMC11360668,Figure_5,oa_package/69/66/PMC11360668.tar.gz,['Postoperative CT scan of an 81-year-old male showing the performed decompression in the coronal plane (red arrow) and axial plane (white arrow) at the L3-L4 and L4-L5 levels.'],Figure 5 Postoperative CT scan of an 81-year-old male showing the performed decompression in the coronal plane (red arrow) and axial plane (white arrow) at the L3-L4 and L4-L5 levels. A: Coronal plane; B: axial plane at the L3-L4 level; C: axial plane at the L4-L5 level.,yes
PMC6507022,Figure_1,oa_package/de/9c/PMC6507022.tar.gz,"['1).', 'a, b The tumor tissue were composed of oval or round mononuclear cells and mixed osteoclast like multinucleated giant cells, with patchy hemorrhage, a few scattered mononuclear cells, and eosinophilic infiltration.', 'There were many megakaryocytesEstimation of EG incidence based on the hospital databaseBecause this study was conducted on a single hospital where the patients were not limited to a specific geographic region, we could not obtain the population-based incidence.']","Fig. 1 , The tumor tissue were composed of oval or round mononuclear cells and mixed osteoclast like multinucleated giant cells, with patchy hemorrhage, a few scattered mononuclear cells, and eosinophilic infiltration. Bone marrow biopsy can be useful in the diagnosis of eosinophilic granuloma (EG). The granulocytic proliferation was active and can be seen in every stage. The erythroid hyperplasia was active, and red blood cell family can be seen. There were many megakaryocytes",yes
PMC5385764,Figure_5,oa_package/fc/91/PMC5385764.tar.gz,"['T1-weighted axial image (A) of the left mid tibia reveals eroded cortex with adjacent soft tissue mass, which is isointense to the muscle and converts to high-intensity signal on axial T2 (B), with a hypointense septation within (arrow)On surgical excision, a lobulated, well-corticated cystic mass was found attached to the mid portion of the left tibia, arising from beneath the periosteum [].', 'Peroperative appearance of lesion.']",Figure 5 Peroperative appearance of lesion. Finding of a lobulated cystic lesion arising from the periostium of left tibia conformed to the diagnosis of a periosteal ganglion made on imaging studies,yes
PMC10541198,Figure_5,oa_package/0f/82/PMC10541198.tar.gz,"['Purified human QR2 (hQR2) was then cocrystalized in the presence of the water-soluble inhibitor (Supplemental Methods; A).', 'YB-537 binds to the catalytic site through a series of hydrophobic and hydrogen bonds with both FAD and amino acids from both QR2 monomers (B).', 'This clearly showed high conservation among the amino acids interacting with FAD (C), while showing far less conservation with those interacting with YB-537 (C).', 'Superposition of the catalytic site of QR2, in complex with YB-537, with QR1 (PDB-ID code 2F1O, B) revealed that,while some catalytic site amino acids were strictly conserved, others such as I129, F132, and N162 in QR2 are Tyr, Met, and His, respectively, in QR1.', 'This, the additional 43-residues of the C-terminus of QR1 (D), and other contrasting features (Supplemental ) may explain the observed more than 6,000-times higher specificity of YB-537 to QR2 compared with QR1.', ', and peak concentrations of 203 ng/g (equivalent to approximately 500 nM YB-537) were detected in the brain approximately one hour after oral administration (Supplemental , A and B).', 'YB-537 bound to hQR2 shows conserved ligand-target interactions that are absent in the closely related QR1.']","Figure 5 YB-537 bound to hQR2 shows conserved ligand-target interactions that are absent in the closely related QR1. ( ) Ribbon representation of the hQR2 homodimer (dimer A, red and dimer B, blue) with stick representation of FAD (yellow) and YB-537 (green). ( ) Critical interactions with amino acids in hQR2 (blue and red) are absent in hQR1 (cyan and pink) due to differences in the amino acid sequence and structure of the 2 enzymes. Specifically, hQR2 has I129, F132, and N162, the latter of which forms an important hydrogen bond with YB-537, that are replaced with Y129, M132, and H162, respectively, in hQR1. ( ) Consurf analysis ( ) showed that the amino acids interacting with the FAD prosthetic group are highly conserved across hQR1 and hQR2, as indicated by the maroon color in the ConSurf representation. However, the amino acids interacting with YB-537 are less conserved, as indicated by the turquoise color in the ConSurf representation. ( ) Ribbon representation of hQR1 dimer (red and blue) and the FAD molecule (yellow). Additionally, a 43 amino acid residue C-terminus is shown in white, which is absent in hQR2. This C-terminus structure may physically hinder YB-537, shown in green, from accessing the catalytic site. The figures were created using the program PyMOL ( ).",yes
PMC9510667,Figure_10,oa_package/f8/91/PMC9510667.tar.gz,[],"FIGURE 10 Brainstem mRNA transcript copy and vocalization peak frequency correlations. NanoString nCounter gene expression (Log2 Counts, -axis) is upregulated in -/- rats compared to wildtype (WT) controls ( -axis) in both males (*** < 0.001; = 3 WT; = 3 -/-) and females (*** < 0.001; = 2 WT, = 3 -/-). For box plots, the boundary of the box closest to zero indicates the 25th percentile. The line within the box marks the median and the boundary of the box farthest from zero indicates the 75th percentile. Whiskers (error bars) above and below the box indicate the 90th and 10th percentiles. Tuba1c relative mRNA quantity (RT qPCR) in the brainstem of male and female rats (WT and -/-). Asterisk demonstrates significant differences (** < 0.01; *** < 0.001). Error bars are SEM, sample sizes are noted in each bar. Tuba1c relative protein density from western blot analysis. Net protein concentrations were normalized to loading control (Gapdh) for male and female rats (WT and -/-). Error bars are standard error of the mean, sample sizes are noted in each bar. Asterisk demonstrates significant differences (* < 0.05). Representative western blot bands for all groups for Tuba1c and reference control Gapdh. Molecular weight (kDa) and mouse brain lysate shown at left for comparison. The average of the top 10 peak frequency of all ultrasonic vocalizations (hertz, Hz, -axis) is negatively correlated to brainstem transcript copy (Log2 counts, -axis) ( = 11). Colored dots indicate sex (males = black; females = gray) and genotype distinctions (closed circles = male; open circles = female). Red regression line is significant correlation ( < 0.05).",yes
PMC11442150,Figure_4,oa_package/4b/87/PMC11442150.tar.gz,"['Drug repurposing in melanoma patient samples.', 'This heatmap is one of 10 visualizations representing the drug-by-cell matrix generated by DrugReSC, with the remaining nine heatmaps presented in Supplementary .', 'Among 24,659 cells from different cell types (A), 1315 cells were selected by PACSI, which were linked to melanoma pathogenesis (', 'After that, we displayed a heatmap of the drug-by-cell matrix constructed by DrugReSC, illustrating pronounced differences in D2C scores among cells with two phenotypes in positive drugs as compared to negative drugs (C and Supplementary ', 'Our analysis revealed significantly higher importance of positive drugs in melanoma compared to negative drugs (D).', 'We observed a significant positive correlation between the drug scores predicted by DrugReSC and the number of results obtained from the PubMed search (E and Supplementary ', 'For example, as depicted in F, one of the 10 melanoma candidate drug lists predicted by DrugReSC was shown (Supplementary Table 14).']","Figure 4 Drug repurposing in melanoma patient samples. (A) the UMAP plot of single-cell data in the melanoma case. (B) the UMAP visualization of PACSI-identified cells. (C) Heatmap displaying the D2C scores of positive and negative drug instances between melanoma-related cells and other cells. This heatmap is one of 10 visualizations representing the drug-by-cell matrix generated by DrugReSC, with the remaining nine heatmaps presented in . (D) box plot showing the average drug scores of positive and negative drugs within the 10 DrugReSCpredicted candidate drug lists for melanoma. A two-sided Wilcoxon rank-sum test was performed to estimate the significance level. (E) Pearson correlation between drug importance scores predicted by DrugReSC for melanoma and the corresponding number of search results (log2(n+1)) on PubMed. This correlation plot is one of 10 generated to illustrate the association between DrugReSC results and available supporting evidence from the literature, with the remaining nine plots provided in . (F) table of the top 10 candidate drugs identified by DrugReSC for melanoma, including the ID of the L1000 project for each drug instance, treatment information, drug score, and FDA approval status. This table is one of 10 tables representing the output candidate drug lists generated by DrugReSC for melanoma, with complete results reported in .",yes
PMC8758478,Figure_7,oa_package/27/82/PMC8758478.tar.gz,"[' 7B).', ' 7A, B).', 'Identification of the holo-Lf binding sites on APP required for holo-Lf-induced amyloidogenic processing of APP.', 'To confirm the capability of these peptides to block amyloidogenic APP fragment production in a cell model, each peptide was dose dependently added to holo-Lf (500 nM; 2 h) before introduction into the media of wt-APP695 SH-SY5Ys for a further 2 h.', ' 7C and Supplementary ', ' 7A).', ' 7D and Supplementary ', 'tif"">Supplementary \nAnnular lesion on right thigh with surrounding vesicles with string of pearls appearance.']",Figure 2 Annular lesion on right thigh with surrounding vesicles with string of pearls appearance.,yes
PMC2742723,Figure_3,oa_package/17/79/PMC2742723.tar.gz,[],"Fig.3 Demonstration of hormone-producing cells in the normal pituitary glands, human ( ) and rat ( ). H&E stained anterior lobe with many round hormone-producing cells. The same section as . The perivascular eosinophilic cells (in ) are positively stained for GH. GH cells (brown) and ACTH cells (blue) are present in close proximity to one another. FSH/LH cells (brown) and PRL cells (blue) are present in tight conjunction (left). These hormone-producing cells contain many dense-cored secretory granules (right).",yes
PMC5840488,Figure_4,oa_package/99/99/PMC5840488.tar.gz,[],"Supplemental FigureS2 Technical validation of immunofluoresence assay and RNA hybridization (ISH) multiplex technique performed on fixed fresh-frozen rhesus macaques cerebrum ( ) cultured with ( ). All images were taken in the same frame and with the same laser intensities. Gray represents nucleic acids (DAPI), red indicates spirochetes labeled with mouse monoclonal outer surface protein A antibodies, green indicates spirochetes labeled with rabbit-derived polyclonal antibodies, yellow represents colocalization of monoclonal and polyclonal antibodies, and blue represents targeting by the respective RNA ISH probe. Spirochetes are highlighted by an intense blue, indicating strong specificity of probe ( probe targeting the 23S rRNA transcript; Advanced Cell Diagnostics catalog number 468211). Colocalization of both monoclonal and polyclonal antibodies and specific RNA ISH probe indicated by concurrent blue, green, red, and yellow fluorescence. Neuropil displays strong punctate blue fluorescence, indicating strong specificity of the rhesus macaque housekeeping gene , with absence of spirochete labeling (rhesus macaques probe targeting the peptidyl-prolyl - isomerase B transcript, catalog number 457711). Faint rare blue fluorescence of neuropil, indicating minimal nonspecific binding of negative control probe, with absence of spirochete labeling ( probe targeting the DapB transcript, catalog number 320871). Scale bar = 20 m ( ). Original magnification, 63 (additional 2 amplification applied with Leica LAS X software when appropriate).",yes
PMC5161749,Figure_8,oa_package/28/de/PMC5161749.tar.gz,[],"Figure7 rs1964911 Is Associated with Increased Risk of Invasive Aspergillosis in HSCT Recipients (A and B) Cumulative incidence of invasive aspergillosis at 24months in recipients (C/C versus A carriers, p= 0.029) (A) and cumulative incidence of invasive aspergillosis at 24months in recipient/donor combination (B) (full model, p= 0.029). (CG) gene expression (C) and production (D and E), NLRP3 expression (F), and IL-1 production (G) in macrophages isolated from peripheral mononuclear blood cells of subjects carrying diverse genotypes at rs1964911 stimulated with conidia. Normalization was performed on Gapdh for real-time PCR and on human -actin for immunoblotting. For immunofluorescence, nuclei were counterstained with DAPI. Photographs were taken with a high-resolution microscope (Olympus BX51). Scale bars, 25m. Data (mean values SD) represent pooled results or representative images (immunofluorescence and immunoblotting) from three experiments. p< 0.01, p< 0.001, CC versus A+ genotype. None, unstimulated cells. See also .",yes
PMC9415962,Figure_2,oa_package/59/6b/PMC9415962.tar.gz,"['Staining for ZIKV E protein was not detected in uninfected and ZIKV heat-inactivated VK2 cells (A).', '1 percent) at six dpi (B).', 'VK2 cells were later infected with ZIKV-UG, which expresses Venus fluorescent protein, and infected cells were monitored throughout the course of infection by Venus fluorescence (C).', 'Since ZIKV-UG established productive infection in VK2 cells (C), we performed this experiment using this strain.', 'hVECs support ZIKV replication and viral production.']","Figure 2 hVECs support ZIKV replication and viral production. ( ) VK2 cells were infected with ZIKV-PR (MOI: 0.5). Uninfected and heat-inactivated ZIKV cells were used as controls. Viral replication was monitored through the presence of the viral envelope (E) protein using an indirect immunofluorescence assay (IFA) with the 4G2 primary antibody and fluorescein isothiocyanate (FITC)-conjugated secondary antibody. DAPI was used to stain the nucleus. ( ) VK2 was infected with ZIKV-PR (MOI: 0.5). The time course of viral replication was analyzed using IFA, as mentioned above. ( ) VK2 cells were infected with ZIKV-UG (MOI: 0.5). Venus fluorescent protein expression cells were visualized with a BioTek LionHeart FX Automated Microscope. The percentages of cells positive for ZIKV-PR were calculated by dividing the average number of ZIKV-infected cells (GFP signal) by the average number of cells. The calculated percentages are located at the lower right corners of the merged images. The total numbers of VK2E6E7 cells expressing the Venus fluorescent protein through ZIKV-UG were quantified using the BioTek LionHeart FX Automated Microscope.",yes
PMC9327617,Figure_5,oa_package/b6/de/PMC9327617.tar.gz,"['The energy of the emitted photons is specific to the element (C), corresponding to the energy difference of the atom shells (i.', 'Raster-scanning the sample enables generation of elemental (iron) maps (E), and can measure multiple elements but cannot directly measure changes related to oxidation state.', 'The incoming beam energy is modulated around the absorption edge of an element of interest (D).', 'The resulting spectrum can provide extra information such as the oxidation state of iron deposits (F).']","FIGURE 5 X-ray methods working principle and example outputs. X-ray fluorescence (XRF) setup, with incoming beam, sample and photon spectrometer collecting emitted photons of all energies. The same setup can be used in X-ray absorptions spectrometry (XAS) or X-ray absorption near-edge structure (XANES), where the incoming beam energy is modulated and output recorded. Incoming photons interact with the sample, and displace inner shell electrons, which are emitted as photoelectrons. When these are replaced by outer shell electrons, the change of energy of the latter leads to the emission of a photon with energy corresponding to the energy difference of the two shells, which is characteristic of the element. Emitted photons are collected by the photon spectrometer. XRF spectrum collected by the photon spectrometer in . The photon count in each energy bin can provide elemental concentrations in the sample. Typical XAS and XANES spectra corresponding to iron. When the beam raster-scans the tissue section (left) and an XRF spectrum is collected for each point, the output can be an elemental map of the sample (here an iron map of a human hippocampus). XANES spectra can be collected for multiple identified iron deposits.",yes
PMC10326119,Figure_2,oa_package/93/6d/PMC10326119.tar.gz,"[' 2(A/B).', 'A and B Example for quantitative assessment of artifacts/artifact reduction in an area with subjectively the most severe artifact appearance (green ROI) and in a distant muscle (red ROI) not affected by the artifact serving as reference in (A) PC-CTstd and in (B) PC-CT130 keV reconstructions.', 'PC-CTstd = photon-counting CT; PT-CT130 keV = PC-CT with monoenergetic reconstructions at 130 keV; HU = Hounsfield unitRadiation doseAll dose calculations were performed using a commercially available dose management and reporting platform (DoseM, INFINITT Europe GmbH).', ' 2).']",Fig. 2 and Example for quantitative assessment of artifacts/artifact reduction in an area with subjectively the most severe artifact appearance (green ROI) and in a distant muscle (red ROI) not affected by the artifact serving as reference in ( ) PC-CT and in ( ) PC-CT reconstructions. and Mean HU values from the ROIs of all patients were compared between PC-CT and PC-CT in the artifact as well as in distant muscle. PC-CT =photon-counting CT; PT-CT =PC-CT with monoenergetic reconstructions at 130keV; HU=Hounsfield unit,yes
PMC6541100,Figure_4,oa_package/22/df/PMC6541100.tar.gz,"['One of these, a two-year-old female, showed progressively worsening general condition and blindness, histologically, perivascular infiltrations of mononuclear cells and deposits of amorphous, eosinophilic, hyaline material around pathogenic organisms (Splendore-Hoeppli phenomenon) (figure 4), a suspected immune-related process with a previous infection was observed but the agent was not identified.', 'Histological section of brain of two-year-old alpaca with non-purulent encephalitis.']","Figure 4 Histological section of brain of two-year-old alpaca with non-purulent encephalitis. Perivascular cuff, deposits of eosinophilic hyaline material consistent with Splendore-Hoeppli material (arrows). Haematoxylin and eosin (H&E) stained, paraffin embedded section. Size bar=100m.",yes
PMC8865601,Figure_1,oa_package/d1/8a/PMC8865601.tar.gz,[' Contrast MRI of the cervicodorsal spine in neutral and flexion1a.'],Figure 1 Contrast MRI of the cervicodorsal spine in neutral and flexion 1a. Sagittal T1-weighted image of the cervical spine showing linear hypointensity in the anterior portion of the lower cervical spinal cord (denoted by white arrow) with associated cord thinning. 1b. Sagittal T2-weighted image in neutral position showing linear hyperintense signal (denoted by white arrow) in the anterior portion of the cervical spinal cord with associated cord thinning. 1c. Axial T2-weighted image at the level of the lower cervical spine showing hyperintense signal in bilateral anterior horn cells giving a Snake Eye Appearance. 1d. Sagittal T1 post-contrast image in flexion shows prominent enhancing posterior epidural space with a flow void (denoted by white arrow).,yes
PMC4717430,Figure_4,oa_package/8c/69/PMC4717430.tar.gz,"['In four samples, the biopsy was scored negative, whereas the resection was positive (figure 4).']","Figure4 pTEN protein expression in (A) resection and (B) matched biopsy specimen, highlighting the difference in expression between both samples. The resection sample was graded as no loss of expression, whereas the biopsy sample was graded as loss of expression (200 magnification).",yes
PMC7482806,Figure_6,oa_package/8e/f8/PMC7482806.tar.gz,[' DHA decreases the frequency of CD8+ effector T (Teff) cells and CD8+ central memory T (TCM) cells in IMQ-induced psoriasis-like mice.'],"Figure 6 Lymph node and spleen cells were isolated from psoriatic mice on day 7 following various treatments. The percentages of CD8 T cells (CD8 CD44 CD62L ) and CD8 T cells (CD8 CD44 CD62L ) in lymph nodes and spleen of the psoriatic mice were determined via FACS. ( ) Shown are representative dot plots of CD8 T and CD8 T cell populations in lymph nodes and spleen. The percentages ( ) or absolute numbers ( ) of CD8 T and CD8 T cells in lymph nodes and spleen of mice were also determined. ( ) On day 24, PASI scores of the skin lesion in psoriatic mice treated with DHA (50 mg/kg) plus administration of CD4 T cells, CD8 T cells or rIL-15 were observed. Briefly, mice received FACS-sorted CD4 or CD8 T cells on day 20, the beginning day of IMQ re-challenging, while others were administered with rIL-15 on days 0, 3 and 6 during initial IMQ priming and DHA treatment. ( ) The percentages of CD8 T cells in lymph nodes of psoriatic mice treated without or with DHA (50 mg/kg) plus rIL-15. All data of column graphs are presented as the mean SD from three separate experiments (n = 5-6 mice/group, * 0.05 and ** 0.01).",yes
PMC8571908,Figure_3,oa_package/f6/66/PMC8571908.tar.gz,['SM: synthesized mammography; S2DM: standard two-dimensional digital mammographyA 47-year-old woman presented with a right breast lump.'],Figure 3 A 47-year-old woman presented with a right breast lump. Mammography demonstrates architectural distortion in the right breast. The distortion is only seen on SM/DBT (arrows). Biopsy confirmed benign breast tissue. SM: synthesized mammography; S2DM: standard two-dimensional digital mammography; DBT: digital breast tomosynthesis,yes
PMC4297388,Figure_1,oa_package/36/12/PMC4297388.tar.gz,"['Comparison of whole mounts of mouse mammary glands derived from three-week old Sox9 cKO mice revealed clear developmental defects ( 1).', 'Notably, however, cKO mammary glands at 8 weeks (and beyond; not shown) displayed minimal or no defects in ductal branching or the extent of overall growth ( 1).', 'Furthermore, the increased branching and alveologenesis associated with pregnancy and lactation in Sox9 cKO was comparable to that of control mice ( 1).', '\nDeletion of Sox9 results in mammary gland defects in 3 and 5 weeks old mice.', 'Sox9 is essential for mouse mammary stem and luminal progenitor cell functionThe gradual recovery of the developmental phenotype seen in Sox9 cKO mammary glands beyond 8 weeks of age is suggestive of either a transient role of Sox9, or an inability of Sox9-null stem/progenitors to contribute to progeny and compensation by stem/progenitors that did not undergo Cre-mediated Sox9 deletion due to mosaic MMTV-Cre mediated gene deletion.', 'Our findings that mammary gland development is delayed in MMTV-Cre driven Sox9 cKO mice ( 1) strongly suggested a potential role of Sox9 function in MaSCs and/or lineage-restricted progenitors.', 'Our conclusion that Sox9 is an essential component of the mammary gland developmental program is based on a substantial delay in post-natal mammary gland ductal elongation and branching in mice with MMTV-Cre directed Sox9 deletion ( 1).']","Figure 1 Whole mount analysis of mammary glands from control (MMTV-Cre; Sox9 ) and Sox9 cKO (MMTV-Cre; Sox9 ) mice. Mammary glands were harvested at 3, 5, 8weeks, mid-pregnancy, lactation day 1, and lactation day 10 and stained with carmine alum and analyzed under the microscope.",yes
PMC10132855,Figure_19,oa_package/54/fe/PMC10132855.tar.gz,[],"Figure 19 On presentation, the left sole showing ulcer with multiple discharging sinuses and yellow granules (white arrows).",yes
PMC1190207,Figure_3,oa_package/ca/36/PMC1190207.tar.gz,['Astrocyte activation following A infusion.'],"Figure 3 Astrocyte activation following A infusion. WT and IL1raKO mice were infused with A or vehicle, and brains prepared as in Figure 2. A) Levels of the pro-inflammatory astrocyte-derived cytokine S100B showed a similar degree of upregulation in A-infused IL1raKO and WT mice. B) Numbers of GFAP-positive astrocytes were increased to a similar degree in both WT and IL1raKO mice infused with A. (error bars = SEM; p > 0.05 between A-infused IL1raKO and WT mice).",yes
PMC5058734,Figure_1,oa_package/5e/11/PMC5058734.tar.gz,"['Increased xCT expression in active TB patients(A) Expression of xct in peripheral blood samples from HC (n = 20), LTBI (n = 20), and TB (n = 20) was determined by qRT-PCR.', ') (Supplementary ).']","Figure 1 Increased xCT expression in active TB patients ( ) Expression of in peripheral blood samples from HC ( = 20), LTBI ( = 20), and TB ( = 20) was determined by qRT-PCR. Relative gene expression was normalized to GADPH. ( ) xCT protein levels in PBMC from HC ( = 3) and TB ( = 3) were determined by immunoblotting. Bar graphs showing semi-quantification of xCT level relative to -actin level, respectively. ( ) Peripheral blood was stained with anti-CD3, CD14, CD16 and xCT antibody and analyzed by flow cytometry. (C) Histogram showed expression of xCT in different cell types from peripheral blood. (D) xCT expression on CD14 CD16 and CD14 CD16 cells from active TB and HC. (E) The percentage of xCT expressing on CD14 CD16 , CD14 CD16 and PMN cells from active TB ( = 10) and HC ( = 13). ( ) Detection of xCT expression in infiltrated immune cells around tuberculous granuloma of lung tissue from patients with active tuberculosis by immunohistochemistry. The brown cells were the xCT positive cells. The one-way ANOVA NewmanKeuls Multiple Comparison Test was used for statistical analyses to compare the differences among multiple groups. Unpaired -test was used to analyze the difference between two groups. * < 0.05, ** < 0.01, *** < 0.001.",yes
PMC8785419,Figure_2,oa_package/fe/68/PMC8785419.tar.gz,"['Convolutional neural network (CNN) is a popular subgroup of DL networks, widely applied in CMR, as it is designed to work with imaging data ().', 'Pipeline of a convolutional neural network (CNN).']","Figure 2 Pipeline of a convolutional neural network (CNN). A CMR image functions as input to the CNN. The CNN identifies and classifies the various attributes (features) of the image for analysis in a procedure named Feature Extraction, including a stack of convolutions and pooling operations. In the convolution operation different-level features, such as edges, colour, gradient orientation are extracted from the input image. The pooling layer reduces the dimensionality of the convolved features, in order to decrease the computational requirements. The nodes in the fully-connected layer are connected directly to all nodes in the previous layer. This layer compiles the data extracted by previous layers and applies various filters to form the final output.",yes
PMC10366742,Figure_3,oa_package/60/4b/PMC10366742.tar.gz,"['After the placement of the naso-biliary tube, a CT scan with diluted iodinated contrast agent administered through a tube to obtain exclusive enhancement of the intrahepatic biliary tract was performed and direct communication between the biliary branch for the 7th segment and the voluminous subcapsular intrahepatic cyst with fistulization into the right pleural cavity was detected (A,B).', '(A,B) CT performed with diluted iodinated contrast agent administered through the naso-biliar tube to obtain an exclusive enhancement of the intrahepatic biliary tract, and coronal (A) and sagittal planes (B).']","Figure 3 ( , ) CT performed with diluted iodinated contrast agent administered through the naso-biliar tube to obtain an exclusive enhancement of the intrahepatic biliary tract, and coronal ( ) and sagittal planes ( ). CT confirmed the presence of bile in a subcapsular intrahepatic cyst, showing the direct fistula with the right pleural cavity (arrows).",yes
PMC8610120,Figure_7,oa_package/30/de/PMC8610120.tar.gz,"['478E-06) was lower in HCC patients ().', 'The methylation levels of the YTH domain family in HCC tissues.']","Figure 7 ( ) The methylation values of ( ) YTHDC1, ( ) YTHDC2, ( ) YTHDF1, ( ) YTHDF2, ( ) YTHDF3 were evaluated using the Ualcan database.",yes
PMC6108014,Figure_2,oa_package/d8/88/PMC6108014.tar.gz,"['Microscopic examination showed cells with abundant granular cytoplasm arranged in small nests surrounded by a thin fibrovascular stroma ().', '1177_2374289518780500-fig2""/>Questions/Discussion Points, Part 3The gross and histological appearance of the tumor confirms the diagnosis.', 'Polygonal to spindle-shaped chief cells surrounded by sustentacular cells give rise to the nesting pattern ().']","Figure 2. Polyhedral tumor cells with eosinophilic granular cytoplasm and ovoid nuclei are arranged in a characteristic alveolar zellballen or nesting pattern surrounded by thin fibrovascular septa. Residual adrenal cortex is present at the periphery (*). H&E, intermediate magnification.",yes
PMC8543940,Figure_2,oa_package/63/13/PMC8543940.tar.gz,"[' 2a to c).', ' 2d, e).', ' 2g, h).', ' 2f), which was positive for PAS (', ' 2i).', ' 2j).', ' 2k and Additional file 1 ', ' 2l).', ' 2l).', 'Other microscopic observations of the SCA34 patient brain and spinal cordMicroscopic observation of the pons and cerebellum from an autopsy case with SCA34.', 'Scale bar = 5 mm (a and l), 500 m (b, d, and g), 100 m (c, f, h, and k), 50 m (e and l), and 20 m (j)Light microscopic observation of white matter vacuoles in an autopsy case with SCA34.']","Fig. 2 Microscopic observation of the pons and cerebellum from an autopsy case with SCA34. Semi-macroscopic view of the pons after Klver-Barrera (KB) staining. Arrowheads indicate severe atrophy of the pontine base. An arrow indicates demyelination of the transverse fibers. Transverse fibers between the medial lemniscus and pyramidal tract showed severe degeneration (magnified from the rectangle area in ). Further magnified view of the transverse fibers between the medial lemniscus and pyramidal tract (magnified from the rectangle area in B). Very scarce and degenerated fibers were observed. , , and After KB ( and ) and HematoxylinEosin (HE) ( ) staining, the most ventral part of the pontine base also showed severe degeneration of pontocerebellar fibers and neuronal loss. Cells with irregular shaped dense nuclei and violet cytoplasm after KB staining as shown in (magnified from the rectangle area in ) or pale gray after HE staining (arrows in and magnified in its inset) were observed. and immunostaining for CD68. Strongly CD68-positive cells were widespread (white and black arrowheads in ) in the pontine base, excluding the pyramidal tracts. These cells occasionally accumulated around the vasculature (white arrowheads in and ). is a magnified view from the rectangle in . The inset in H illustrates a magnified view from the rectangular area in the center. The cytoplasm of the CD68-positive cells was positively stained with periodic acid-Schiff (PAS) (arrowheads). V denotes vessels. Double staining with PAS (red) and anti-CD68 (brown) illustrates co-staining for PAS and CD68 in the same cells (arrowheads). The double-staining is shown as a dark-brown color, except for wall of the vasculature, which only stained for PAS. These macrophages were positive for Gallyas silver impregnation method. Semi-macroscopic view of the cerebellum showing white matter atrophy and mild and uneven demyelination (arrow). Scale bar=5mm ( and ), 500m ( , , and ), 100m ( , , , and ), 50m ( and ), and 20m ( )",yes
PMC7322487,Figure_2,oa_package/b5/6f/PMC7322487.tar.gz,"['MRI demonstrated a moderate right hip effusion with multiple hypointense intra-articular bodies and no evidence of erosion or donor sites ().', 'Case 2.', ' Case 3: A 38-year-old female with no relevant past medical history presented with a six-month history of progressive left hip pain and limping.']","Fig. 2 Case 2. Pelvic MRI sequences in a 26-year-old female. MRI demonstrates a moderate right hip joint effusion with multiple hypointense intra-articular bodies. (a) Coronal STiR sequence, (b) sagittal PD fat-suppressed sequence of the right hip, (c) axial T2 fat-suppressed sequence of the right hip.",yes
PMC3678744,Figure_3,oa_package/07/9b/PMC3678744.tar.gz,"['We can see that applying an affinity constraint limits the selection of pixels to those which belong to the background, resulting in the shortest path, one which circumvents obstructionsWe can see from the examples in , the end result of our algorithm is a set of connected pixels (shown in red), which travel from the POI q to .', 'The algorithm proceeds by the following:The MS signature overlaid on tumor regions in an (a) ovarian, (b) prostate H, (c) breast HE, and (d) prostate HE image.', 'Table 4Bayesian classifier AUC for ms in distinguishing stromal from tumoral lymphocytes for S1-S3Qualitative EvaluationIn , we have presented MS signatures in red/green overlaid on both tumor and non-tumor regions for representative images from all three datasets.', 'a reveals that, for a very complex region, the MS paths become increasingly tortuous as they adapt to the local heterogeneity.', '(l) illustrates the unique MS signature for the POI located in a stroma region, but bounded on the left and right sides by the tumor.']","Figure 3 The MS signature overlaid on tumor regions in an (a) ovarian, (b) prostate H, (c) breast HE, and (d) prostate HE image. Corresponding results for benign regions are shown in (i-l), respectively. Three rays from each image (e-h) and (m-p) are extracted and illustrated beneath the corresponding images. We can see that in the presented non-tumor regions (i-l), the MS signature has fewer and smaller objects to obstruct its path, and thus the rays are less tortuous, unlike in the corresponding tumoral regions (a-d)",yes
PMC8548695,Figure_4,oa_package/ec/74/PMC8548695.tar.gz,[],"Figure4 macrophages have an inflammatory phenotype. RNAseq data of selected differentially expressed cytokine and cytokine receptor genes identified by Reactome pathway analysis. Heatmaps of differentially expressed interferon cytokines and receptors and IL-12-family of cytokines and receptors from RNAseq analysis, Z-score normalized.",yes
PMC7275990,Figure_2,oa_package/2b/ec/PMC7275990.tar.gz,"['Kidney biopsy (Fig 2) showed 36 glomeruli on light microscopy with 11 globally sclerosed, and 5 others with FSGS with focal collapsing features demonstrating variable collapse of the glomerular tuft with overlying podocyte activation/hypertrophy.', ' 2(A) Low-power view demonstrates diffuse acute tubular injury and interstitial edema.']","Figure2 (A) Low-power view demonstrates diffuse acute tubular injury and interstitial edema. One glomerulus (arrow) demonstrates a collapsing variant of focal segmental glomerulosclerosis (FSGS) (periodic acidSchiff stain; original magnification,100). (B). Higher-power image of a glomerulus shows characteristic features of collapsing FSGS. There is collapse of the glomerular tuft with marked activation/hypertrophy of the overlying podocytes, many of which contain prominent protein resorption droplets in their cytoplasm (periodic acidSchiff stain; original magnification,200). (C). Electron photomicrograph shows global podocyte foot-process effacement (uranyl acetate stain; original magnification,8,000),",yes
PMC8601817,Figure_4,oa_package/f4/21/PMC8601817.tar.gz,"['According to different alpha diversity indices, as shown in s 4(c) and 4(d), compared with the Sham group, the species richness (Ace index) of the I/R group was significantly increased (P 0.', '003"" position=""float""/>Composition of microbiota: (a) OTU numbers, (b) OTU Venn analysis, (c) Ace index ( P 0.']","Figure 4 Composition of microbiota: (a) OTU numbers, (b) OTU Venn analysis, (c) Ace index ( < 0.05 and < 0.01), (d) Shannon index ( < 0.05 and < 0.01), (e) principal component analysis (PCA) for the weighted UniFrac distance of the gut microbiota, and (f) heat map analysis of intestinal flora 16S rRNA detection genus level. The data were presented as meansSD; =6 rats per group.",yes
PMC8077270,Figure_3,oa_package/2a/07/PMC8077270.tar.gz,[],"FIGURE 3 Ascending malignancy: A, 8days postexcisional biopsy with proximal erythema and new mass; B, intraoperative view: subsequent incisional biopsy; C, intraoperative photograph of ELD from receiving tertiary care center",yes
PMC7515510,Figure_1,oa_package/fa/0d/PMC7515510.tar.gz,"['During dilator advancement, seen in , resistance was encountered at the cavoatrial junction.', 'Digital subtraction angiography from operating room fluoroscopy.']",Figure 1 Digital subtraction angiography from operating room fluoroscopy.,yes
PMC5053626,Figure_4,oa_package/fe/0a/PMC5053626.tar.gz,"['The membrane-associated Ccnd1 promotes Ral-GTPase activationA.', 'Similar results were obtained using prostatic cancer cells (Supplementary ).']","Figure 4 The membrane-associated Ccnd1 promotes Ral-GTPase activation HEK293T cells were co-transfected with HA-RalB and either HA-Ccnd1, or HA-Ccnd1-CAAX or an empty vector as a negative control. Twenty-four hours after transfection, active RalB-GTP was affinity purified with RalBP-beads from cell lysates and detected by immunoblotting with anti-HA antibody. HA-D1, HA-RalB and HA-RalB-GTP amount from a representative experiment is shown. RalBP-beads and actin were used as loading controls. The experiment was independently repeated four times. Relative mean values sem for the HA-RalB-GTP/ total HA-RalB ratio are plotted. Significance values were determined by one way ANOVA and Holm -statistic post-test (* < 0.05; ** < 0.01). R3327-5A rat cells were infected with interference shRNA against RalB (shRalB) or with scramble (scr) as a control. Relative invasion values are expressed in mean sem. Data are from three independent experiments. Significance values were determined by one way ANOVA and Holm -statistic post-test (** < 0.01). Immunoblot showing the levels of expression of RalB in (C). Gel stain was used as a loading control.",yes
PMC9403194,Figure_4,oa_package/c9/a6/PMC9403194.tar.gz,"['A further 3 episodes post-operative chemotherapy using the same agents was completed ().', 'Microscopic image showed large amount of well differentiation chondrocytes and osteoblast hyperplagia.', 'The patient had been reassessed at 1-2-3 6-12-24 and 32 month post-operative.']",Fig. 4 Microscopic image showed large amount of well differentiation chondrocytes and osteoblast hyperplagia.,yes
PMC7615119,Figure_16,oa_package/60/36/PMC7615119.tar.gz,[],"Fig.6 Myc loss prevents the endothelial activation and single-cell states but not vascular pathology. , GSEA hallmark analysis for each single-cell cluster. b, GSEA hallmark analysis performed with the bulk proteome and transcriptome. c, Heatmaps showing log(fold change) ofgenes and proteins belonging to different sets. d, Barplot showing the NES in each single-cell cluster for the indicated gene sets. e, Barplot with the top differentially expressed (DE) proteins in livers. f, Enrichment analysis showing a significant positive enrichment in translational initiation-related genes and proteins encoded by genes that are regulated by the Myc-Max transcription factors. g, Micrographs showing immunostainings for the Myc protein, which is upregulated in liver ECs (ERG cells) after deletion. h, mRNA expression (normalized counts from bulk RNA-seq). i, Experimental layout for the inducible deletion of and in Cdh5 and iSuRe-Cre ECs and scRNA-seq analysis. j, UMAPs and barplot showing the ten identified clusters and the percentage of cells for each cluster in the different samples. k, Dot plot of the top upregulated genes in liver ECs belonging to the indicated gene marker groups. l, GSEA hallmark analysis showing the decreased expression of most gene sets in . m, Double deletion of and in ECs results in a significant reversion of proliferation (Ki67 ERG cells) and Esm1 expression (Esm1 ERG ) to control levels. n, 3D confocal micrographs from thick vibratome sections (top) or thin sections (bottom), and liver macroscopic pictures showing vessel enlargement and liver pathology in mutants similarly to mutants. Data are presented as mean values s.d. For statistics, see Source Data File 1. Scale bar, 100 m, except n lower panel, 1 mm.",yes
PMC3522639,Figure_3,oa_package/46/aa/PMC3522639.tar.gz,"['COL1A1 mRNA expression was strikingly different in freshly isolated control and CHF fibroblasts (A, left), consistent with in vivo changes.', 'After culture for 48 hours, the mRNA expression of COL1A1 was comparable for the two groups (A, right).', 'Similarly, COL3A1 mRNA showed 5-fold greater expression for freshly isolated CHF fibroblasts versus controls (B).', 'After 48 hour culture COL3A1 mRNA expression also became indistinguishable between the two groups, although unlike COL1A1, culture appeared to decrease COL3A1 mRNA expression (B).', 'control (C).', 'g003Comparative gene expression studies with freshly isolated versus cultured control (CTL) and CHF dog fibroblasts.', 'We found it interesting that collagen I and collagen III were differentially regulated in cell culture (A/B).', 'Thus, collagen-III expression is not significantly altered after 48 hours in culture () and peaks after 7 days of culture (), whereas collagen-I expression increases significantly after 48 hours () and appears to peak around 4 days of culture ().']",10.1371/journal.pone.0052032.g003,yes
PMC11634337,Figure_3,oa_package/f0/f8/PMC11634337.tar.gz,"['An MRI performed 367 days after IRE revealed no suspicious lesions ().', 'Magnetic resonance imaging (T2-weighted sequence) performed 367 days after irreversible electroporation shows no suspicious lesions.']",Figure 3 Magnetic resonance imaging (T2-weighted sequence) performed 367 days after irreversible electroporation shows no suspicious lesions. The ablation area appears with marked hypointensity (white arrow),yes
PMC10684955,Figure_4,oa_package/88/37/PMC10684955.tar.gz,"[', 2007), qualitative analysis showed that GFAP-labeling (s 4A,E) and 4G8-labeling (s 4B,F) were found in close proximity (s 4D,H) in the dorsal subiculum of both Oil and VCD injected mice.', 'The GFAP-labeled astrocytes mostly surround clusters of 4G8-labeling, especially in the VCD SWDI mice (s 4D,H).', 'Moreover, blood vessels, identified by CD31 immunolabeling (s 4C,G), were closely associated with GFAP-labeled cells in the parenchyma but few blood vessels overlapped regions with dense GFAP and 4G8-labeling (s 4D,H).', 'Qualitative analysis shows A , astrocytes and blood vessels are located in spatial proximity in the caudal subiculum of SWDI mice.']","Figure 4 Qualitative analysis shows A, astrocytes and blood vessels are located in spatial proximity in the caudal subiculum of SWDI mice. Section from SWDI oil-treated mouse shows labeling of 4G8 , GFAP , and vascular marker CD31 . A merged image shows overlapping labeling for 4G8, GFAP and CD31. As shown in the inset (area in dashed box) labeling for 4G8 (green chevron) is seen in proximity to labeling for astrocyte (blue arrow head) and vascular (magenta arrow head) markers. Section from SWDI VCD-treated mouse shows labeling for 4G8 , GFAP , and CD31 . A merged image shows overlap of 4G8, GFAP and CD31 , which can be seen at a higher magnification in the area bounded by the dashed box (inset). Bars =200 m. Bar =75 m.",yes
PMC4647021,Figure_2,oa_package/81/51/PMC4647021.tar.gz,"['Elbow arthroscopy revealed the olecranon fossa covered by the fat pad, and a portion of the fat pad was noted to be detached from the rest of the hypertrophied fat pad (s 2(a) and 2(b)).', '001""/>Posterior arthroscopic view of the left elbow.']",Figure 2 Posterior arthroscopic view of the left elbow. (a) The olecranon fossa is covered by the fat pad (asterisk). (b) The fat pad is partially detached from the rest of the fat pad. (c) The olecranon fossa is visible when the fat pad is pulled upwards. (d) View after debridement of the posterior fossa of the elbow. O: olecranon; H: humerus; T: triceps; F: olecranon fossa. Image views: top: proximal; left: lateral; right: medial; bottom: distal.,yes
PMC5027634,Figure_5,oa_package/44/5b/PMC5027634.tar.gz,"[')When graft-derived cells were found in the vicinity of the central canal or the pia mater, they often formed neural differentiated tissues, which were further classifiable into two groups according to their maturation level (Table 1A and Figs.', ' 5b).', ' 5a).', ' 5c).', ' 5d).', ' 5a.']","Fig. 5 Representative images of DNT, UDNT, and BLT. , Representative images of the DNT with zone formation around the central canal resembling cysts, which were observed in the 1231A3 NR-NSPC transplanted spinal cords 6months after transplantation. The red arrows indicate the cyst, blue arrows indicate the VZ, and black arrows indicate the IZ. Upper panel: H&E, STEM121, hNestin, and hGFAP. (Scale=500m.) Lower panel: STEM121, hNestin, and hGFAP. (Captured in boxed area 1 in the first panel. Scale=100m.) ( ) STEM121+ scattered neural cells (SNCs) observed in the MZ that formed in the rostral end of boxed area 2 in Fig.5A are indicated by white arrows. There were many more migrating neural cells than is indicated. (Scale=50m.) ( ) Representative histologic features of the UDNT, as observed in a 1201C1 EB-NSPCs transplanted spinal cord at the 12th week after transplantation. Upper panel: STEM121 and H&E. (Scale=500m.) Lower panel: H&E, hNestin, hGFAP, hKi67, Alcian Blue, and HNA. (Captured in the boxed area in the upper panel. Scale=50m.) ( ) Representative histologic features of the BLT as observed in a 1210B2 NR-NSPC transplanted spinal cord at the 12th week after transplantation. The low power field views of the STEM121 staining is the same image in Fig. . Upper panel: STEM121 and H&E. (Scale=500m.) Lower panel: H&E, hNestin, hGFAP, and hKi67. (Captured in the boxed area in the upper panel. Scale=50m.)",yes
PMC4484509,Figure_1,oa_package/83/cd/PMC4484509.tar.gz,[],"Fig.1 Histopathological findings of multiple organs from RV-infected fetuses; the liver shows congestion, expansion of the portal area with inflammatory infiltrate and piecemeal necrosis, vacuolar degeneration of epithelial cells in the intrahepatic bile ducts (a, b), necrotizing and inflammatory changes with apoptotic hepatocytes (so called acidophil bodies; arrows) (c), giant cell formation of hepatocytes (arrows) (d), mild deposition of bile pigments into the hepatocytes (arrows) (e) and erythrophagocytosis by Kupffer cell (inset of e). Mild nephritis with partial hemorrhage in the kidney (f), minor pneumonia accompanied by congestion, alveolar hemorrhage and interstitial edema in the lung (g, h), hypoplasia with hemorrhage in the spleen (i) and lymph node (j), mild myocarditis with interstitial edema in the heart (k) can be seen. H&E stain.",yes
PMC10471457,Figure_2,oa_package/dd/c0/PMC10471457.tar.gz,"['Right eye (OD) (2A) and left eye (OS) (2B) on hospital day seven, at the time of initiating antifungal treatmentOD: oculus dexter; OS: oculus sinisterThe corneal labs came back without significant Pseudomonas growth but were positive for Candida albicans in the right eye (oculus dexter (OD)) and Staphylococcus lugdunensis-oxa ss in the left eye (oculus sinister (OS)).']","Figure 2 Right eye (OD) (2A) and left eye (OS) (2B) on hospital day seven, at the time of initiating antifungal treatment OD: oculus dexter; OS:oculus sinister",yes
PMC6827223,Figure_5,oa_package/8e/ad/PMC6827223.tar.gz,"[' 5a).', 'MOG35 55 EAE in C57Bl/6J (n = 17) vs.', ' , generalized estimating equation association coefficientAs we identified the WT C57Bl/6J mice as a very suitable model for studying retinal injury in EAE, we chose to investigate the functional outcome of the structural results.', ' 5c).', ' 5d).', ' 5e).']","Fig. 5 MOG EAE in C57Bl/6J ( =17) vs. sham-immunized ( =12) mice. EAE clinical scores. Decreased visual acuity of EAE mice compared to untreated C57Bl/6J mice. OKR measurement was carried out for 120days after MOG immunization as described above, area under the curve compared by ANOVA with Dunnetts post hoc test. Thickness of retinal layers. RGC count after 9months. Data expressed as meanSEM, * <0.05; ** <0.01; *** <0.001. values for OCT and RGC data obtained from generalized estimating equation models accounting for within-mouse, inter-eye correlations. Linear regression analyses. IRL thickness during EAE is associated with ultimate neuronal loss (top row) and disease severity (bottom row). Similar results were obtained 5 and 7months after immunization (data not shown). , generalized estimating equation association coefficient",yes
PMC9648822,Figure_4,oa_package/06/19/PMC9648822.tar.gz,"['NOX1 can be induced in various cell types and contributes to adhesion molecule expression of endothelial cellsBecause NOX1 regulates endothelial and immune cell functions,31,32 we sorted CD45+ haematopoietic and CD31+ endothelial cells from the heart of WT mice and assessed Nox1 mRNA expression (A and Supplementary material online, S6).', 'Nox1 mRNA was primarily expressed in CD45+ cells, whereas CD31+ cells showed undetectable to more than 5-fold lower levels of Nox1 mRNA (B).', 'Whereas we could detect Nox1 mRNA in CD64 cells, Nox1 mRNA was undetected in CD11b+CD64+ cells (C).', 'Nox1 expression in different cell types ex vivo and upon LPS or PMA stimulation in vitro.', '3 cells and in response to PMA in THP-1 cells, but not in LPS-stimulated HCASMC (C F).', 'This upregulation was significantly reduced upon knock-down of NOX1 through siRNA (G and H).']","Figure 3 Endothelial activation based on VCAM-1 and ICAM-1 expression and cardiac inflammatory response by staining of Mac-2-, IL-1-, and NLRP3 positive cells 44 weeks after STZ/vehicle injection. ( ) Representative microscopic images for myocardial vessels stained with VCAM-1 and ICAM-1, scale bar: 20 m. ( ) Quantification of VCAM-1 and ICAM-1 expression in coronary arteries and venules. ( ) Representative microscopic images for inflammatory cells stained with Mac-2, IL-1, and NLRP3, scale bar: 20 m; ( ) Quantification of Mac-2-, IL-1-, and NLRP3 positive cells per heart section. Groups were compared using two-way ANOVA with Sidak test, * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001, ( = 10 for WT-CTD, 9 for WT-HFHS, 9 for KO-CTD, 9 for KO-HFHS).",yes
PMC6835632,Figure_3,oa_package/35/f8/PMC6835632.tar.gz,"['The animals were fed either with SD or KD diet, or SD combined with daily gavage administration of ketone supplements for seven days, while blood levels were measured after 24 h and after seven days of treatment ().', '0001) had significantly decreased levels of blood glucose after 24 h, compared to control (A).', '0001; A).', '007) groups after 24 h, compared to control (B).', '007) (B).', '0325), compared to control (C).', '0001) at seven days (D).', 'Changes in blood glucose and R- HB levels of 4-months-old SPD rats after 24 h and seven days of treatment, in post-exercise state.']","Figure 3 Changes in blood glucose and -HB levels of 4-months-old SPD rats after 24 h and seven days of treatment, in post-exercise state. ( ) The change in glucose levels in SPD rats, with exercise, for the baseline, 24 h, and seven days post-intervention. ( ) The corresponding percent change in glucose levels. ( ) The resulting blood -HB levels. ( ) The percent change in the blood -HB levels. Abbreviations: SPD: Sprague-Dawley rat; SD: standard diet (control); KD: ketogenic diet; KE: ketone ester; KS: ketone salt; KSMCT: ketone salt and medium chain triglyceride, 1:1 ratio. *: < 0.05, **: < 0.01, and ****: < 0.0001 level of significance.",yes
PMC4003766,Figure_4,oa_package/bd/c2/PMC4003766.tar.gz,"['Surgical pathology confirmed adenosquamous cell metastasis from his lung cancer (diagnosed in 2011) ().', '003""/>Adenosquamous carcinoma involving adenomatoid nodule.']",Figure 4 Adenosquamous carcinoma involving adenomatoid nodule. (a) 4x. (b) 10x. (c) 20x.,yes
PMC3118207,Figure_1,oa_package/66/95/PMC3118207.tar.gz,['Images of structures before and after treatment.'],"Figure 1 . Neurography of the femoral nerve before treatment showing prolonged latency of the left side. CT image of the pelvis showing a cystic structure 4.8 3.7 mm in size, corresponding to (a) the ileopectineal bursa, medial to (b) the musculus iliopsoas, and dorsal to the (d) musculus sartorius, which caused a medial dislocation of (c) the femoral artery, vein, and femoral nerve. Neurography of the femoral nerve four months after treatment, showing normal latency values.",yes
PMC10731363,Figure_1,oa_package/2a/10/PMC10731363.tar.gz,"['Moreover, the finding (9,10) that IVL is consistent with typical uterine smooth muscle tumors in terms of immune expression and this patient s immunohistochemistry data confirm the homology of IVL with uterine smooth muscle tumors (A-1D).', 'Pathological results of intravascular tumor.']","Figure 1 Pathological results of intravascular tumor. (A,B) HE staining shows that the tumor is nodular, partially involving blood vessels, and the tumors nuclear division is visible, HE 40. (C,D) Desmin and SMA were diffusedly expressed in tumor cells, IHC 40. HE, haematoxylin and eosin; SMA, smooth muscle antibody; IHC, immunohistochemistry.",yes
PMC8471761,Figure_2,oa_package/5b/5a/PMC8471761.tar.gz,"['They possess a thick oocyst wall and central morula that contains 4 6 refractile globular internal structures; because the oocysts are shed unsporulated, sporozoites are not present in freshly passed stool (A) [39].', 'Diagnosis by wet mount microscopy can be enhanced by the use of differential interference contrast (DIC) (C) or ultraviolet (UV) autofluorescence (B) [4].', 'cayetanensis will fluoresce blue (B).', ', iron hematoxylin and trichrome) and appear as colorless refractile spheres that may be wrinkled or collapsed and have a ground glass appearance when viewed over multiple focal planes (D).', 'When the oocysts take up the stain with ZN and MAF, they appear pink to red with a wrinkled appearance (E).', 'Unfortunately, a large percentage of oocysts might not successfully take up the stain, resulting in ghost forms that appear similar to those stained with trichrome (E) [4,20,38,39,41].', 'Staining with the hot safranin method results in more uniform staining of oocysts (F), but it is often not preferred as it requires the heating of the stain [20,38,40].', 'org/1999/xlink"" xlink:href=""microorganisms-09-01863-g001"" position=""float""/>Oocysts of Cyclospora cayetanensis in stool specimens observed under different staining methods.']",Figure 2 Oocysts of in stool specimens observed under different staining methods. ( ) unstained concentrated wet mount. ( ) UV autofluorescence. ( ) differential interference contrast (DIC). ( ) trichrome stain. ( ) Kinyouns modified acid-fast. ( ) modified safranin. (Figures courtesy of the CDC-DPDx).,yes
PMC1820611,Figure_3,oa_package/c9/9d/PMC1820611.tar.gz,"['In both cases, a quantitative comparison of protein levels demonstrated that the Trp690Ser and Trp690Gly mutants were expressed in human fibroblasts to similar levels as the wild-type counterpart (A and 3B).', 'Moreover, the repair-deficient mutants with Ala substitutions at codons 531, 542, 585, 690, and 733 were detected in human fibroblasts in nearly identical amounts as wild-type XPC protein (C).', 'Normal Cellular Expression and Localization of Repair-Deficient XPC Mutants(A) Immunoblot analysis of XP C fibroblasts transfected with pcXPC vectors coding for wild-type protein or repair-deficient mutants.', 'A time course experiment with the wild-type sequence demonstrated that expression of the XPC GFP fusion increases during incubation periods of 18 h after transfection, with a cellular localization that is predominantly restricted to the nucleus (D).', 'Control cells transfected with vector pGFP demonstrated that GFP alone displays a more diffuse distribution extending to both the cytoplasma and nucleus (E).', 'However, the strong nuclear localization is reestablished after expression of GFP fused to the Trp690Ser mutant (F).', 'A similar level of fluorescence with the same characteristic nuclear localization was recorded for each of the repair-defective Ala mutants (G).']","Figure 3 Normal Cellular Expression and Localization of Repair-Deficient XPC Mutants (A) Immunoblot analysis of XPC fibroblasts transfected with pcXPC vectors coding for wild-type protein or repair-deficient mutants. Soluble cell lysates (20 g) were separated on polyacrylamide gels and probed for XPC protein using a specific monoclonal antibody directed against the C-terminal sequence of human XPC. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was recorded as the internal standard. Lane 2: XPC fibroblasts transfected with the pcDNA control vector. (B) Densitometric quantification of three to five independent experiments. The intensity of immunoreactive bands corresponding to XPC protein was normalized against the glyceraldehyde-3-phosphate dehydrogenase standard and reported as the percentage of the wild-type signal ( SD). (C) Immunoblot analysis of XPC fibroblasts transfected with pGFP (lane 1), or the vectors coding for wild-type (lane 2) or mutant XPC proteins (lanes 37) fused to GFP. The primary antibody was directed against GFP. (D) Time course of fluorescent fusion protein expression in XPC fibroblasts transfected with pXPCGFP containing the wild-type sequence. Nuclei were stained with Hoechst reagent. (E) Distribution of GFP in XPC fibroblasts. (F and G) Representative images demonstrating the nuclear localization of repair-deficient mutants 15 h after transfection.",yes
PMC10864737,Figure_3,oa_package/66/76/PMC10864737.tar.gz,[],"FIGURE 3 Axial computed tomography scan image of the chest, mediastinal window showing massive right pleural effusion, pleural thickening and left pleural nodule, highly suggestive of metastatic disease.",yes
PMC10825618,Figure_1,oa_package/48/a7/PMC10825618.tar.gz,"['Furthermore, the biopsies were obtained in a standardized protocol from 4 different regions in each lung (): superolateral, superomedial, inferolateral and inferomedial.', '3D volume rendering reformat from a postmortem chest CT from our study (using RadiAnt DICOM Viewer 2022.', '2.']","Fig. 1 3D volume rendering reformat from a postmortem chest CT from our study (using RadiAnt DICOM Viewer 2022.1 Software 3D Preset: Bones and Skin 3) shows how the postmortem expired lungs (in blue) have their anterior lower limits around the 4th or 5th intercostal spaces. From this reconstruction, it is possible to understand why the 2nd anterior intercostal space was used for the tissue sample collection of the upper regions and the 3rd anterior intercostal space was used for the tissue sample collection of the inferior regions. The limit between the lateral and medial regions was the midclavicular line. The 4 regions of access to tissue sample collection are also shown: SL = superolateral; SM = superomedial; IL = inferolateral; IM = inferomedial.",yes
PMC7713145,Figure_2,oa_package/c7/d0/PMC7713145.tar.gz,"[' 2a).', ' 2b, c).', '', '05Increased astrogliosis but not microglial activation in the PD substantia nigraIn the substantia nigra, quantification of the number of enlarged amoeboid HLA-DR+ microglial cells (activated) revealed no significant difference between controls, PDND and PDD brains (']","Fig.2 Quantification of -synuclein, tau and amyloid- pathology in multiple brain regions. There was in increase in -synuclein pathology in PDD compared to PDND cases in the hippocampus (MannWhitney U test, =0.015), entorhinal (MannWhitney U test, =0.015), and occipitotemporal cortex (Unpaired t test with Welchs correction, =0.037). There was no difference in tau pathology between groups in any region (KruskalWallis test with Dunns correction for multiple comparisons, >0.05). There were no between-group differences in amyloid- pathology (KruskalWallis test with Dunns correction for multiple comparisons, >0.05). AMG: Control n=10, PDND n=13, PDD n=11, HIPP/ERC/OTC: Control n=8, PDND n=13, PDD n=10, PFC: Control n=13, PDND n=15, PDD n=7, PPC: Control n=13, PDND n=17, PDD n=11. Parkinson's disease no dementia, Parkinson's disease dementia, amygdala, Hippocampus, entorhinal cortex, occipitotemporal cortex, prefrontal cortex, posterior parietal cortex. * <0.05",yes
PMC5215693,Figure_2,oa_package/db/f6/PMC5215693.tar.gz,['Acute MS cases lack C3d+ microglial clusters.'],"Figure 2 Acute MS cases lack C3d+ microglial clusters. Actively demyelinating lesions in an acute MS case (acute MS#2), showing (A) loss of Luxol fast blue (LFB, asterisk) staining, and (B) accumulation of CD68+ macrophages in the lesion core (asterisk). Lesion edge is indicated by the line in A,B. (C) Immunoreactivity for the myelin oligodendrocyte glycoprotein (MOG) showed a general loss of staining within the lesion (asterisk), (D) with MOG+ myelin degradation products (arrows) at the lesion edge and in the lesion core. (E) Immunostaining for proteolipid protein (PLP), showing a patchy pattern (asterisk), indicative of active demyelination. (FI) Tlymphocyte immunostaining, showing (F) cuffing of CD3+ Tlymphocytes around blood vessels and (G) abundance in the parenchyma. (H) Most of these Tlymphocytes were CD8+ whereas (I) CD4+ staining, although present, was less pronounced and detected only at the perivascular spaces. (J) Myelinassociated glycoprotein (MAG)+ myelin products were found in the lesion core (arrows) where (K) CD68+ cells had phagocytic morphology (arrows), and (L) at the lesion edge (arrows) where (M) we observed CD68+ cells of morphology consistent with activated microglia (black arrow) and phagocytic macrophages (white arrows). (N) Periplaque area with normal appearing white matter (NAWM), showing sparse ionized calciumbinding adapter molecule 1 (IBA1)+ microglia with nonreactive/nonphagocytic morphology, (O) confirmed by the small morphology of CD68 reactive cells in the same area. C3/C3b/iC3b/C3d immunostaining, showing (P) abundant immunoreactivity within macrophages in the lesion core, (Q) in a staining pattern consistent with debris. Scale bars: (A,B) 200 m; (C,E,G,N,P) 50 m; (H,I,K,M,O) 20 m; (F,J,L,Q) 10 m; (D) 5 m. Hematoxylin was used as counterstain in CQ. [Color figure can be viewed at ]",yes
PMC6202881,Figure_1,oa_package/a9/e9/PMC6202881.tar.gz,['MerciecaVGrechVDeGiovanniJVUse of stents for correction of pulmonary artery branch stenosisImages Paediatr Cardiol200463110.'],Figure 1. Transverse view of the thorax showing significant compression of the right pulmonary artery (PA). Superior vena cava (SVC) and pulmonary artery are indicated with arrows.,yes
PMC6883672,Figure_7,oa_package/5d/7b/PMC6883672.tar.gz,"['The effect of BLM and TREP on ERK,\np38 and JNK phosphorylation is presented in (a f).', 'BLM induced phosphorylation\nof I B inhibitory subunit leading to NF- B activation (g and h).', 'JNK and I B \nphosphorylation were inhibited by treprostinil (e and g) and these data were\naccordingly quantified to check statistical significance (', '.', '1177_2045894019881954-fig7""/>DiscussionAs reported, in this study an experimental animal model of IPF was used to test the\nefficacy of a stable prostacyclin analogue when administrated through the inhaled\nroute.']","Fig. 7. Orotracheal administration of treprostinil (TREP) reducesphosphorylation of IB and JNK. (a, c, e, g): Representative Westernblots for p-ERK, p-p38, p-JNK and p-IB in mouse lung homogenatesof samples collected 21 days post bleomycin (BLM)-challenge orvehicle (VEH). Total levels of MAPKs and actin were used as loadingcontrols. (b, d, f, h): Quantification of protein levels afternormalization is shown for each molecule. One-way ANOVA was used forcomparison among groups and was followed by Tukeys multiplecomparisons test. Data are expressed as meansSEM. Differencesbetween BLM/VEH and SAL/VEH groups are shown by ( <0.05); differences betweenBLM/VEH group and BLM/TREP group are shown by *. SAL: saline.",yes
PMC11509894,Figure_4,oa_package/70/04/PMC11509894.tar.gz,"['Available preclinical studies indicate that targeting claudins, especially claudin-1, may be a promising therapeutic approach in thyroid cancer ().', 'Targeting Claudin-1 and Claudin-4 in Other MalignanciesThe therapeutic strategies targeting claudin-1/4 are intensively investigated in experimental oncology ().', 'Therapeutic strategies targeting claudin-1 in thyroid cancer.']",Figure 3 Survival differences in groups of low and high expression of ( ) claudin-1 ( = 0.001) and ( ) claudin-4 ( = 0.07).,yes
PMC7509698,Figure_6,oa_package/a4/31/PMC7509698.tar.gz,"['AP radiograph from pre-magnetic resonance imaging arthrogram injection shows a well-defined ossicle in the pathway of the anterior bundle of the UCL consistent with chronic trauma (arrow)Valgus extension overload syndrome and posteromedial impingement in a 23-year-old professional pitcher, presenting with posteromedial elbow pain, post ulnar collateral ligament (UCL) reconstruction.']","Figure 6 Valgus extension overload syndrome and posteromedial impingement in a 23-year-old professional pitcher, presenting with posteromedial elbow pain, post ulnar collateral ligament (UCL) reconstruction. T1-weighted magnetic resonance imaging shows small posteromedial osteophytes at the olecranon (arrow). Note UCL graft (arrowhead)",yes
PMC8488983,Figure_1,oa_package/d5/28/PMC8488983.tar.gz,"['The area best representing the lesion were annotated as the Region Of Interest (ROI) ().', '3 OD ().', 's and tables.']","Figure 1. Example of quantification, by digital pathology analysis, of smooth muscle actin (A) and desmin (B) expression in a case of AMH. Whole scanned slides were obtained using an objective of 5x using the Aperio LV1 scanner (Leica Biosystems, Inc, Buffalo Grove, IL, USA) and processed in QuPath (V 0.2.0-m8, University of Edinburgh, UK). Regions of interest (ROIs) were selected in fields showing full thickness of the gallbladder wall with diagnostic findings. ROIs (yellow) were analyzed for positive (red) and negative (blue) pixel quantification. Bar: 1 mm.",yes
PMC11590213,Figure_3,oa_package/5d/63/PMC11590213.tar.gz,"[' 3A); treatment with P021 had no effect on progenitor cell proliferation (', ' 3A).', ' 3B,C), and treatment with P021 did not restore the survival of newborn cells (', ' 3B,C).', '', 'Fisher s LSD test after one-way ANOVATo further assess the effect of P021 on neuron survival, we evaluated pyramidal neuron cell density in the CA1 layer of the hippocampus of treated versus untreated mice.', ' 3D), and P021 did not improve the survival of pyramidal neurons in Cdkl5 /Y mice (', ' 3D).', ' 3E,F), Cdkl5 /Y mice had a reduced percentage of mature spines compared to wild-type mice (', ' 3E,G).', ' 3E,G).']","Fig.3 Effect of chronic oral P021 treatment on neuronal survival and dendritic spine maturation in the brain of KO mice. Number of BrdU-positive cells in the subgranular zone (SGZ) of the dentate gyrus (DG) of hippocampal sections from vehicle-treated (+/Y =4,/Y =3) and P021-treated (/Y+P021 =4) male mice. Number of DCX-positive cells in the upper granular layer of the DG of hippocampal sections from vehicle-treated 5+/Y ( =3) and/Y mice ( =3) and from P021-treated 5/Y mice ( =4). Examples of hippocampal sections processed for DCX immunostaining of one animal from each experimental group. Number of NeuN-positive cells in the CA1 field of hippocampal sections from vehicle-treated 5+/Y and/Y mice and from P021-treated 5/Y mice as in B. Examples of Golgi-stained hippocampal pyramidal neurons of one animal from each experimental group; red arrows are examples of mature mushroom-shaped spines. Dendritic spine density in hippocampal pyramidal neurons of vehicle-treated (+/Y =3,/Y =3) and P021-treated (-/Y+P021 =3) male mice. Data are expressed as number of spines per 10m. Percentage of mature dendritic spines in relation to the total number of protrusions in hippocampal pyramidal neurons of 5+/Y and 5/Y mice as in F. Values are represented as meansSEM. ** p<0.01, *** p<0.001. Fishers LSD test after one-way ANOVA",yes
PMC11254415,Figure_3,oa_package/cd/15/PMC11254415.tar.gz,[],"Fig.3. and IL-17A synergistically induce ANGPTL4. (A to D) gene and ANGPTL4 protein expressions in AGS cells (A), human primary GECs (C), or mouse primary GECs (D) infected with WT (MOI = 100) in the presence or absence of IL-17A (100 ng/ml) (24 h), or in AGS cells infected with WT (MOI = 100) in the presence of IL-17A (50, 100, and 200 ng/ml) (24 h) (B) were analyzed by real-time PCR, ELISA, and Western blot ( = 5). (E) IL-17A protein expression in gastric mucosa of -infected ( = 131) and uninfected donors ( = 50) or in gastric mucosa of -infected ( = 74), -infected ( = 57), and uninfected donors ( = 50) was compared. (F) IL-17A protein expression in gastric mucosa of -infected patients with mild ( = 34), moderate ( = 45), and severe inflammation ( = 24) and with normal gastric histopathology ( = 28) was compared. (G) The correlation between ANGPTL4 protein and IL-17A protein in gastric mucosa of -infected patients was analyzed. (H) gene and ANGPTL4 protein expressions in gastric mucosa of WT -infected WT and mice at 12 weeks p.i. were compared ( = 5). (I) gene and ANGPTL4 protein expressions in mouse and human gastric organoids infected with WT (MOI = 100) in the presence or absence of IL-17A (100 ng/ml) (24 h) were analyzed by real-time PCR, ELISA, and Western blot ( = 5). (J) AGS cells were pretreated with signal pathway inhibitors and then infected with WT (MOI = 100) in the presence of IL-17A (100 ng/ml) (24 h). ANGPTL4 protein in AGS cells was compared ( = 5). (K and L) AGS cells were pretreated with or without BAY 11-7082 and then infected with WT (MOI = 100) in the presence or absence of IL-17A (100 ng/ml) (24 h). gene expression and ANGPTL4, p65, and p-p65 proteins were analyzed by real-time PCR, ELISA, and Western blot ( = 5). (M) AGS cells were pretreated with anti-IL-17A and/or aiti-IL-17RA Abs and then infected with WT (MOI = 100) in the presence of IL-17A (100 ng/ml) (24 h). gene expression and ANGPTL4, p65, and p-p65 proteins were analyzed by real-time PCR, ELISA, and Western blot ( = 5). Data are representative of 2 independent experiments. Data are shown as mean SEM and analyzed by Students test, MannWhitney test, and one-way ANOVA. Western blot results are run in parallel and contemporaneously. * < 0.05, ** < 0.01, n.s. > 0.05 for groups connected by horizontal lines.",yes
PMC10044858,Figure_5,oa_package/95/53/PMC10044858.tar.gz,['\nComputerized tomography scan of a 61-year-old male patient with colon carcinoma and liver metastases.'],"Figure 5 The intensity histograms of regions with and without metastases are different; hence, the first order radiomics features[ ], which are based on the intensity histogram will potentially be different.",yes
PMC9301608,Figure_9,oa_package/98/a1/PMC9301608.tar.gz,"['In patients with LFS, particularly close monitoring for osteosarcoma and other sarcomas (), breast cancer, adrenocortical carcinoma, leukemias, and tumors of the central nervous system is recommended.', '47-year-old woman with Li Fraumeni; axial contrast-enhanced fat-suppressed T1-weighted MRI demonstrates a 3 cm heterogeneously enhancing mass in the deep masticator space, which was a recurrent grade 3 myofibrosarcoma.', 'Current international consortium protocols recommend that patients with the LFS-associated gene mutation should undergo abdominal ultrasounds every 3 4 months up to age 10-year-old, as well as annual WB-MRI and annual brain MRI with contrast throughout their entire lives [65], [66].']","Fig. 9 47-year-old woman with Li Fraumeni; axial contrast-enhanced fat-suppressed T1-weighted MRI demonstrates a 3cm heterogeneously enhancing mass in the deep masticator space, which was a recurrent grade 3 myofibrosarcoma.",yes
PMC5523466,Figure_5,oa_package/f3/55/PMC5523466.tar.gz,"['In the saline-treated eyes, characteristics consistent with OIR persisted at P45 in the hyperoxia (s 5(e) and 5(g)) and IH (s 5(i) and 5(k)) groups.', 'These high levels correlated with characteristics consistent with ROP () and are consonant with previous findings of a late reactivation phenomenon of ROP after intravitreal Avastin [17].', '004""/>Retinal flatmounts showing ADPase-stained retinas from 45-day-old rats.']","Figure 5 Retinal flatmounts showing ADPase-stained retinas from 45-day-old rats. Groups are as described in . Images are 10x magnification. Scale bar, 100 m.",yes
PMC5585743,Figure_2,oa_package/ab/aa/PMC5585743.tar.gz,['(A) Hippocampal volume assessment.'],"Figure 2 Hippocampal volume assessment. The gray matter was segmented from T1 images . The hippocampus, isolated using automated anatomical labeling, was interpolated to individual gray matter images . A mean volume index of all voxels of the hippocampal regions was computed for each subject. The main effect of disease on the left hippocampal volume. The aMCI subjects had smaller left hippocampal volumes than the control subjects. The interaction of disease and cognitive change on the left hippocampal volume. The aMCI declining group displayed smaller hippocampal volumes than the aMCI stable group. The aMCI declining group had smaller hippocampal volumes than the control declining group. The interaction of disease and cognitive change on the right hippocampal volume. The aMCI declining group had smaller right hippocampal volumes than the control declining group. The bars are presented with the hippocampal volumes. The error bars represent the standard errors of the means of the hippocampal volumes. * < 0.05. aMCI, Amnestic mild cognitive impairment; RGD, remitted geriatric depression.",yes
PMC3302888,Figure_1,oa_package/5c/73/PMC3302888.tar.gz,"['Visual inspection of co-registered PET/MR images revealed distinct activity retention in the cortex of all transgenic mice corresponding to their study group, whereas for all control animals the cortex appeared to be free of specific activity uptake ().', 'g001Small-animal [11C]PiB PET/MRI overview.', 'g001""/>Time-activity curves (TACs) of target and reference tissues showed characteristics that were common for all study groups ( and Extracerebral tracer retention in proximity to brainWe observed considerable [11C]PiB retention in regions of the mouse head that appeared to be extracerebral, possibly around nasal and eye cavities, but very close to the brain ().', 'To quantify the dynamic data, TACs with high initial time resolution (162 frames: 120 1 s, 24 10 s, 8 30 s, 10 300 s) were used ().', 'The shown coordinates are identical to those shown in .']",10.1371/journal.pone.0031310.g001,yes
PMC9505492,Figure_7,oa_package/76/78/PMC9505492.tar.gz,"['05, a,b).', '05, c e).', '05, g,h).', 'Lactobacillus rhamnosus JYLR-005 decreases intestinal permeability and inhibits LPS/TLRs/NF- B pathway activation induced by HRZE.']","Figure 7 JYLR-005 decreases intestinal permeability and inhibits LPS/TLRs/NF-B pathway activation induced by HRZE. ( ) Serum concentration of FD4 (n = 5 per group); ( ) serum concentration of LPS ( = 6 per group); ( ) TNF-, ( ) IL-1, ( ) IL-6, ( ) TLR2, ( ) TLR4, and ( ) NF-B mRNA levels of liver tissue in each group ( = 6 per group). The data are presented as the means SEM ( = 6 per group). The HRZE + JYLR-005 group and the HRZE + MgIG group shared the control group and the HRZE group. Compared with the control group: * 0.05, ** 0.01, *** 0.001, and **** 0.0001. Compared with the HRZE group: < 0.05, < 0.01, and < 0.0001.",yes
PMC1665232,Figure_11,oa_package/0d/2a/PMC1665232.tar.gz,[],"Figure 11 CT showing very large, well-defined soft tissue tumour arising in the pelvic peritoneum.",yes
PMC10530667,Figure_3,oa_package/d3/f1/PMC10530667.tar.gz,"['After preincubation with 50 M CGS12066B, the rise in [Ca2+]i in HUVEC in response to 30 M BW723C86 increased three five times (A,B).', 'In a separate experiment, the cells were preincubated with different concentrations of CGS12066B for 5 min, and after that, we determined the response to 30 M BW723C86 (C).', 'After preincubation with 50 M CGS12066B, BW723C86 causes an increase in [Ca2+]i already at 5 M, and at 10 M, the effect of BW723C86 reaches its maximum (D).', 'In the presence of CGS12066B [Ca2+]i, elevation occurs at 1 3 M of 5-HT and further increases at 10 100 M (E).', 'Potentiation by CGS12066B of [Ca2+]i rise in HUVEC in response to BW723C86 and 5-HT.']","Figure 3 Potentiation by CGS12066B of [Ca ] rise in HUVEC in response to BW723C86 and 5-HT. ( ) Kinetics of [Ca ] rise in response to BW723C86 (30 M) in the presence or absence of CGS12066 (50 M). Dashed line arrow indicates the addition of CGS12066B or vehicle (0.1% DMSO in PSS). ( ) The values of calcium signals of HUVEC in response to CGS12066B, BW723C86, or both. ( ) Effects of different concentrations of CGS12066B on [Ca ] rise induced by BW723C86 (30 M). [Ca ] rises in response to different concentrations of BW723C86 ( ) or 5-HT ( ) after preincubation without or with CGS12066B (50 M). * < 0.05; ** < 0.01 when compared to CGS12066B and BW723C86 in ( ) and to the corresponding controls in ( ).",yes
PMC10690071,Figure_2,oa_package/0c/d5/PMC10690071.tar.gz,"['Intraoral image of the patient showing lesion in the palatal region (arrow)Coronal section of computed tomography showing uniform hypodense lesion with no erosion of the palatal bone (arrow) Under general anesthesia, incision markings were done for clearance of one centimeter around the lesion ().']",Figure 2 Intraoral image of the patient showing lesion in the palatal region (arrow),yes
PMC4855003,Figure_3,oa_package/c3/a4/PMC4855003.tar.gz,"[' shows the resected colon after total colectomy.', '002""/>Total colectomy.']",Figure 3 Total colectomy.,yes
PMC9648440,Figure_5,oa_package/32/5d/PMC9648440.tar.gz,"[' 5).', ' 5a).', ' 5b, c), presumably due to the lowest viral load among all tested groups.', ' 5b).', 'Heatmap analysis of interferon and inflammation signaling pathways in nasal turbinates.', 'Intranasal immunization of Syrian hamsters with Nsp1-K164A/H165A induced potent humoral response and protected against WA1/2020 challengeBased on the data obtained from above studies, Nsp1-K164A/H165A appears to be the most attenuated recombinant virus and hence was chosen for subsequent evaluation of immunogenicity and efficacy as a LAV candidate.']","Fig. 5 Heatmap analysis of interferon and inflammation signaling pathways in nasal turbinates. Genes associated with inflammation ( ), interferon-alpha response ( ), interferon-gamma response ( ), and TLR responses ( ) are presented in this figure. Each solid square represents one hamster. Five groups (uninfected, WA1/2020, PRRA, Nsp1-K164A/H165A, and Nsp1-N128S/K129E) were coded in light charcoal, blue, red, green, and purple, respectively. The data represents the Z-scores derived from FPKM values of RNAseq transcriptomic analysis. Red, positive Z-Score denotes upregulation and blue, negative Z-score for downregulation. Genes in the yellow box were those that are specifically upregulated in Nsp1-K164A/H165A-infected hamsters. Genes in the green box were those that were expressed to a lesser degree in Nsp1-K164A/H165A group than in WA1/2020 or PRRA or Nsp1-N128S/K129E groups.",yes
PMC3955295,Figure_4,oa_package/c7/9d/PMC3955295.tar.gz,"['H E Staining, 100X.']","Figure 4. Sulphur Granules of Actinomyces With Peripheral Mixed Inflammatory Reaction. H&E Staining, 400X",yes
PMC8584484,Figure_3,oa_package/51/9f/PMC8584484.tar.gz,"['Herein, the imaging findings of a 66-year old male with metastatic NSCLC investigated before (A C) and after 3 cycles of pembrolizumab (D F).']","Figure 3 Herein, the imaging findings of a 66-year old male with metastatic NSCLC investigated before ( ) and after 3 cycles of pembrolizumab ( ). An overall response to treatment is easily visible on MIP (maximal intensity projection) images ( , ), including a complete metabolic remission of all bony lesions (( ); white hollowed arrow). On the contrary, morphological imaging proved the appearance of a new bone lesion in the first lumbar vertebra (( , ); white arrows), which in fact corresponded to a healed metastasis on PET/CT ( , ). Note also the appearance of diffuse thyroid uptake (( ); red arrow), consistent with thyroiditis, one of the irAEs that typically predicts treatment response and good patients outcome.",yes
PMC6900980,Figure_2,oa_package/c7/50/PMC6900980.tar.gz,"['The number of cells isolated per hemisphere were recorded as shown in C.', '6-fold decrease in A (1-42) in PS1-APP-CX3CR1+/ mice compared with PS1-APP mice (A) (7.', 'Levels of A (1-40) were also significantly reduced in PS1-APP-CX3CR1+/ mice (B).', 'Partial CX3CR1 deficiency affects A levels but does not affect total microglial numbers or the numbers of plaque-associated microglia.', 'Partial CX3CR1 Deficiency Does Not Affect Microglial Number and Association With Plaques of the Same SizeTo determine if partial CX3CR1 deficiency affects overall microglial numbers in the brain, we quantified the total number of microglia harvested per hemisphere of PS1-APP-CX3CR1+/ and PS1-APP mice and found no statistically significant differences between the two genotypes (C).']","Figure 2 Partial CX3CR1 deficiency affects A levels but does not affect total microglial numbers or the numbers of plaque-associated microglia. Quantification of A (1-42) and A (1-40) by ELISA in brain homogenates of 10-month old mice (Numbers represent mean ng of A/mg brain SEM). Quantification of total number of microglia isolated per hemisphere. Quantification of the number of CD11b+ cells associated per plaque 75 m in diameter. Representative micrographs of plaques (green) and microglia (brown) in PS1-APP and PS1-APP-CX3CR1 mice. For all measurements in this figure, = 6 for PS1-APP, = 7 for PS1-APP-CX3CR1 . For 80 plaques were counted per genotype. * represent statistically significant.",yes
PMC7665439,Figure_2,oa_package/18/cd/PMC7665439.tar.gz,['Myocardial histology showing active lymphocytic myocarditis with necrosis and areas of organization.'],"Figure 2 Myocardial histology showing active lymphocytic myocarditis with necrosis and areas of organization. The scale bar in the left upper corner is as follows: ( ) 100 m; ( ) 50 m; ( ) 50 m; and ( ) 20 m. ( ) Haematoxylin and eosin (HE) staining, magnification 10. ( ) Azan staining, magnification 20. ( ) HE staining, magnification 20. ( ) HE staining, magnification 40.",yes
PMC5582693,Figure_9,oa_package/11/46/PMC5582693.tar.gz,"['1177_1941738117694841-fig9""/>0.']","Figure 9. Ultrasound of the normal rectus abdominis muscles in short axis, with the muscle belly (R) surrounded by the rectus sheath, with the linea alba seen at the midline (arrow). The transversalis fascia runs along the posterior aspect of the rectus abdominis muscles.",yes
PMC10742296,Figure_2,oa_package/e9/24/PMC10742296.tar.gz,"['We term the tumour cells and TILs located in the inner tumour cluster as intratumoural tumours cells and intratumoural TILs ().', 'Main components of digital NPC-TILs.']",Figure 2 Main components of digital NPC-TILs. Inner and outer tumour cluster. Intratumoural and stromal TILs.,yes
PMC4263445,Figure_2,oa_package/35/f1/PMC4263445.tar.gz,"['In PBS-treated neurons, microtubules and mitochondria can be seen in axons with (A) and without varicosities (, B and C).', 'p- -Syn aggregates in the axons of neurons 14 d after PFF addition (day in vitro [DIV] 19) were visualized using horseradish peroxidase (HRP) conjugated anti-mouse secondary antibody (, D and E) and immunogold (, F I).', 'We found that the -syn aggregates did not fill the width of the axonal cytoplasm with (, D F) or without (, G I) varicosities, suggesting that transport of organelles along the axon could occur.', ' -Syn aggregates do not impair fast axonal transport of synaptophysin or mitochondriaTo test the hypothesis that -syn aggregates do not block axonal transport, we performed live-cell imaging of organelle markers in primary hippocampal cultures derived from wild-type (i.', 'Moreover, abnormal endosomes with nonuniform internal vesicles could also be seen embedded within p- -syn positive aggregates (, B and C, arrows).']","FIGURE 2: Ultrastructure of endosomes in -syn inclusionbearing axons. (A, D, E) Immuno-EM of HRP-labeled p--syn inclusions in axons. (B, C, FI) Immunogold-labeled p--syn inclusions in axons. (AC) Three examples of PBS-treated neurons. No HRP immunoreactivity was found in PBS-treated controls. (DI) Six examples of neurons 14 d after PFF exposure. p--Syn aggregates were visible with HRP (asterisks). Immunogold labeling allowed visualization of the 10- to 15-nm filamentous -syn inclusions. Note that the inclusions did not fill the entire axonal cytoplasm. Arrows point to examples of membrane organelles juxtaposed to the aggregates. Scale bar, 500 nm.",yes
PMC9445312,Figure_3,oa_package/d2/4b/PMC9445312.tar.gz,['PET/CT images of the patient.'],"Figure 3 PET/CT images of the patient. Increased 18F-FDG metabolism showed in the thyroid and cervical lymph nodes on the ( ) transverse, coronal and ( ) sagittal sections. The orange arrow indicated one of the cervical metastatic lymph nodes.",yes
PMC6826269,Figure_1,oa_package/16/6f/PMC6826269.tar.gz,"[' presents the whole intestinal wall at the dimension showing the greatest cancer area.', 'CO;2-C8194005.']","Figure 1. Assessment of a cancer volume on a radiological image. Schematics of (A) the components of the radiological donut-shaped image (gray area), (B) before and (C) after NAC. CT imaging of the radiological donut-shaped image (D) before and (E) after NAC. NAC, neoadjuvant chemotherapy; SD, standard deviation.",yes
PMC8095242,Figure_2,oa_package/ae/28/PMC8095242.tar.gz,"['Axial high resolution computed tomography showed diffuse bilateral ground-glass and alveolar consolidation.', ' CT angiography was performed, which did not show any signs of PTE.']",Fig.2 Axial high resolution computed tomography showed diffuse bilateral ground-glass and alveolar consolidation.,yes
PMC8365522,Figure_4,oa_package/ff/ae/PMC8365522.tar.gz,"['The luciferase activities of the JAZF1 3 UTR wild-type reporter were significantly reduced in 293T cells transfected with miR-182-5p mimic ( 4A).', ' 4JAZF1 is a downstream target of miR-134-5p and circPTK2 and is involved in lipolysis and adipogenesis.', 'Through IHC analysis, we presented that the JAZF1 level was significantly upregulated in the adipose tissues of cachectic patients ( 4B).', 'By contrast, the amount of lipid droplets significantly decreased when JAZF1 was overexpressed ( 4C).', 'Conversely, the adipogenesis markers were downregulated compared to the control group ( 4D).', 'Western blotting analysis further confirmed these results ( 4E).', 'The FFA content in the culture medium significantly decreased when JAZF1 was downregulated and increased when JAZF1 expression was overexpressed ( 4F).']",Figure4 JAZF1 is a downstream target of miR-134-5p and circPTK2 and is involved in lipolysis and adipogenesis. (A) Schematic of the predicted miR-182-5p binding site in the JAZF1 3 UTR. Luciferase activity of wild-type or mutated JAZF1 3 UTR in 293T cells after co-transfection with miR-182-5p or miRNA control. (B) Representative IHC staining of JAZF1 expression in adipose tissues from patients without/with CAC (Scale bar: 150m). (C) Oil Red O staining of lipid accumulation in adipocytes (6d) with knockdown/overexpression of JAZF1 (Scale bar: 360m). (D) qPCR results of the expression of adipose-related markers in adipocytes (6d) with knockdown/overexpression of JAZF1. (E) Western blot analyses of the expression of adipose-related markers in adipocytes (6d) with knockdown/overexpression of JAZF1. (F) Concentration of FFA released in culture medium by adipocytes with knockdown/overexpression of JAZF1.,yes
PMC6570649,Figure_2,oa_package/6c/f9/PMC6570649.tar.gz,"[' 2a), bladders were fixed in formaldehyde, paraffin embedded, sectioned and stained with hematoxylin and eosin.', ' 2b).', 'There is no obvious bladder pathology in 8-months after repetitive moderate TBI.', 'Conscious cystometry indicates overactive bladder phenotype in rmdTBI miceWe evaluated urodynamic function using a conscious cystometry (CMG) approach, which enabled us to perform multiple tests on each animal (']","Figure 2 There is no obvious bladder pathology in 8-months after repetitive moderate TBI. ( ) Two-month-old WT mice were subjected to sham-operation or rmdTBI but were also treated with either mAb or IgG isotype control. Experimental treatment setup (Red arrows, rmdTBI, Black arrows, antibody/IgG injection; green lines, bladder histopathology). ( ) Bladder sections of sham, rmdTBI+IgG, and rmdTBI+ mAb mice were subjected to hematoxylin & eosin staining at 8 months after injury. Inset images are high magnifications of representative areas with corresponding images above. L=Lumen, U=Urothelium, D=Detrusor. Scale bar, 20m.",yes
PMC10899809,Figure_1,oa_package/61/45/PMC10899809.tar.gz,"['In Case 1, lobulated infiltrative soft tissue mass centered within the right upper medial thigh adductor muscles.']","Figure 1 In Case 1, lobulated infiltrative soft tissue mass centered within the right upper medial thigh adductor muscles. (A) The tumor appears hypointense in axial T1WI. (B) Shows heterogeneous hyperintense signal in axial T2WI with FS. (C) Pre-gadolinium axial dry gradient image shows intermediate to low signal intensity of the mass. (D) Post-gadolinium axial image shows avid heterogeneous enhancement of the mass and demonstrates the extension of the lesion into the right side perineum. FS:Fat Suppression",yes
PMC9150229,Figure_3,oa_package/7f/c1/PMC9150229.tar.gz,"['5 cm within the setting of a normal LV ejection fraction of 55% and normal wall motion ().', '1177_23247096221101852-fig3"" position=""float""/>.']",Figure 3. Transthoracic echocardiogram with contrast shows 2 mobile echo-density masses in the left ventricular apical region measuring 1.8 1.2 cm (red arrow) and 1.0 0.5 cm (yellow arrow).,yes
PMC5999462,Figure_5,oa_package/b6/ec/PMC5999462.tar.gz,['Pathological characteristics and immunohistochemical findings in the tumor in the fourth surgery.'],"Figure 5 Pathological characteristics and immunohistochemical findings in the tumor in the fourth surgery. Metastasis tumor of cervical lymph nodes after the fourth operation. (A) Low-power view showing, in some regions, a solid arrangement of tumor cells and a low differentiated carcinoma structure. (B) High-power view showing obvious cell atypia. (C) Positive CK7 adenocarcinoma marker staining. (D) Ki67 staining indicated a high proliferation index, indicating actively proliferating cancer cells. (E) CK5/6 and (F) p63 stainings in the tumor showed negative staining for squamous cell carcinoma markers. (G) The blue arrow shows metastasis of the tumor in the marginal sinus of the lymph node. The red arrow indicates residual lymphoid tissue. (H) The blue arrow indicates a small number of tumor cells that are arranged in a gland tubular structure. The red arrow indicates that most of the tumor cells are flaky. (I) The nucleus is large, and it is vacuolated and polymorphous. The nucleoli are obvious, and mitotic image is visible. The blue arrow shows a mitotic image.",yes
PMC6555498,Figure_2,oa_package/a1/c5/PMC6555498.tar.gz,['Angiography of the left coronary artery (left and middle image) and right coronary artery (right image).'],Figure 2 Angiography of the left coronary artery (left and middle image) and right coronary artery (right image). Left coronary angiography showing spontaneous coronary artery dissection of the distal left anterior descending artery with TIMI 2 flow (arrows). TIMI -Thrombolysis in Myocardial Infarction,yes
PMC3226673,Figure_1,oa_package/46/b7/PMC3226673.tar.gz,['Histomorphological signs for disc degeneration.'],Figure 1 . increase of cell density (chondrocyte proliferation) with moderate clones of chondrocytes (arrows) in the NP of a 42 years old patient (score 4). severe occurrence of granular changes (arrows) in the NP of a 67 years old patient (score 3). structural alterations with tears and clefts (arrows) in the AF of a 54 years old patient (score 3). severe increase in acid mucopolysaccharides (mucous degeneration) with dark blue staining areas around clones of chondrocytes (arrows) in the NP of a 77 years old patient. (a-c H&E stain; d Alcian blue-PAS stain/a-d scale bar 100 m).,yes
PMC8773081,Figure_6,oa_package/ed/0e/PMC8773081.tar.gz,"['To evaluate the efficacy of blocking activin A, we treated SARS-CoV-2 infected hamsters with a combination of REGEN-COV cocktail and the anti-activin A antibody, REGEN-COV cocktail alone and the anti-activin A antibody alone (A).', 'Here, the anti-activin A antibody was used in preventative mode, administration starting 2 days prior to SARS-CoV-2 challenge, while REGEN-COV cocktail was used in treatment mode, administration starting on day 1 following challenge (A).', '00467-21-f006"" position=""float""/>As previously shown, treatment with the low dose of REGEN-COV cocktail (5 mg/kg) was protective and reduced body weight loss in all SARS-CoV-2 challenged hamsters (B).', 'There was no additional benefit of the anti-activin A antibody for the body weight loss prevention (B).', 'Five out of 10 SARS-CoV-2 challenged hamsters that were treated with the isotype control antibodies lost more than 20% body weight and were euthanized unscheduled on day 7 following challenge (D).', 'Treatment with REGEN-COV cocktail alone or in combination with the anti-activin A antibody was fully protective from severe morbidity (D).', 'Treatment with the anti-activin A antibody alone was partially protective from severe morbidity and in this group, three out of 10 hamsters were euthanized unscheduled on day 7 due to the excessive body weight loss (D).', 'Although difference in the survival between the anti-activin A antibody treated group and the isotype control treated group was not statistically significant (D).', 'SARS-CoV-2 induced marked to severe inflammation in the lungs of hamsters treated with the isotype control antibodies (C; Table 4).', 'Severity of lung inflammation was notably reduced in the groups treated with REGEN-COV cocktail alone or in combination with the anti-activin A antibody (C; Table 4); none of the animals in these groups showed severe lung inflammation.', 'The anti-activin antibody did not impact the severity of SARS-CoV-2 induced lung inflammation (C; Table 4).', 'Antibody treatment schedules are schematically shown in A and 7A.', 'At the end of experiments (A and 7A), hamsters were euthanized under sedation with isoflurane followed by tissue collection.', 'Detailed description of these analyses are provided in figure legends ( to 8).']","FIG 6 Efficacy of the anti-activin A antibody alone, REGEN-COV antibody cocktail alone, and the combination of the anti-activin antibody and REGEN-COV cocktail in the golden Syrian hamster model of SARS-CoV-2. (A) Study design overview and the antibody administration schedule. SARS-CoV-2 challenge was administered to groups 1 to 4 on day 0 ( =10 per group). Group 5 received PBS and served as a healthy control ( =6). (B) Daily body weight changes; (C) lung inflammation grading scores; (D) survival curves. In panel B, data are mean standard error of mean (SER). The axis represents percent body weight change from the baseline, 2 days (day -2) prior to SARS-CoV-2 challenge. axis represents days prior and postchallenge. Black asterisks indicate mean body weight differences between groups 1 (black line) and 4 (green line). Red asterisk indicates mean body weight difference between Groups 2 (red line) and 4 (green line). In panel C, the axis represents semiquantitative scores for lung inflammation. Data are mean standard deviation from the mean (SD). Asterisks indicate difference between SARS-CoV-2 challenged groups. In panel D, the axis represents percentage of surviving hamsters. axis represents days following SARS-CoV-2 challenge. Statistical analyses were conducted with a one-way ANOVA followed by Tuckeys multiple-comparison tests. SARS-COV-2 challenged groups were compared. , < 0.05; , < 0.01; , < 0.001; ****, < 0.0001.",yes
PMC3299701,Figure_3,oa_package/9c/c2/PMC3299701.tar.gz,"['SWI of the phase maps derived from T2 W imaging yielded excellent visualization of the small venous irregularities and enhanced contrast between normal tissue, focal inflammatory lesions and the microvasculature as highlighted in \nB\n.', 'Notably, the structural irregularities observed in T2W or T2 W images corresponded with areas of inflammatory pathology both in cerebrum (\nC\n) and cerebellum (\nD\n) as revealed by conventional hematoxylin and eosin (H E) histology.', 'Indeed, the lesions that we could visualize with MRI show the same appearance and localization characteristics of CD4 T cells (\nD\n).', 'g003T2 W hypo-intense regions correspond to cellular infiltrates detected by histology.']",10.1371/journal.pone.0032796.g003,yes
PMC7507173,Figure_1,oa_package/85/cc/PMC7507173.tar.gz,[],"FIGURE 1 A, Appearance of the edema of the left cheek at the initial visit. B, Ultrasound image of the left cheek clearly showing a hypoechogenic area, suspect for abscess and possibly a fistula. C, An abscess and decayed second bicuspid in the left side of the upper jaw. D, Radiological examination of tooth nr 25 showing deep caries with a decayed part of the crown",yes
PMC11389364,Figure_4,oa_package/01/b0/PMC11389364.tar.gz,"['0092) chemokines (A and B) compared to C57BL/6 mice.', 'No differences in the production of TNF- , IL-6, IFN- , and IL-17, cytokines associated with a positive outcome in IRIS patients (22, 23), were detected in brain homogenates of 5-LO / mice compared to C57BL/6 mice at day 35 post-infection (C through F).', 'Chemokines associated with high mortality in C-IRIS patients are reduced in 5-LO-deficient mice during C.']",Fig 4 Chemokines associated with high mortality in C-IRIS patients are reduced in 5-LO-deficient mice during infection. Cytokines within brain homogenates of C57BL/6 and 5-LO mice infected with 52D were measured on day 35 post-infection. Data shown are expressed as mean SEM and are cumulative of three experiments utilizing three to four mice per group. CCL2 and CCL3 were significantly lower in brain homogenates of 5-LO mice compared to C57BL/6 mice. Significant differences were defined as 0.05.,yes
PMC11508844,Figure_8,oa_package/98/8f/PMC11508844.tar.gz,"['The nominal sizes of the Evoque valve are based on the outer frame diameter of the valve; these diameters are compared to annular dimensions calculated at CT using the projected perimeter derived diameter (PDD, ).', 'Additionally, CT scan is used to assess the position and the distance of papillary muscles relatively to the annulus: from one side papillary muscles can interfere with valve positioning if they are too close to the valve; from the other side, they can be considered another retention mechanism, thus they can stabilize the ventricular side of the valve after deployment ().', 'Screening for TTVR, key imaging features.']",Figure 6 Intraprocedural guidance of T-TEER with TEE ( ) or ICE ( ). See text for details.,yes
PMC5642507,Figure_2,oa_package/89/c2/PMC5642507.tar.gz,"['Representative immunohistochemical analysis of -amyloid and phospho-Tau in the OB across AD stages(A) Control: negative staining of -amyloid in the anterior olfactory nucleus (AON).', 'This transcriptomic fingerprint might partially corroborate the unmodified activation state of phosphorylated CREB (Ser133) observed at olfactory level during AD progression (Supplementary ).', 'On the other hand, other hubs proposed by the interaction network analysis such as TGF-beta and c-Jun presented unmodified protein levels across AD grading (Supplementary ).']",Figure 2 Representative immunohistochemical analysis of -amyloid and phospho-Tau in the OB across AD stages ( ) Control: negative staining of -amyloid in the anterior olfactory nucleus (AON). Low AD: mild compact deposits of -amyloid in the AON. Intermediate AD: sparse neuritic plaques of -amyloid in the AON. High AD: mild neuritic and diffuse plaques of -amyloid in the AON. ( ) Control: isolated neuropil threads of phospho-Tau protein in the glomerular layer of the OB. Initial AD: Moderate neuropil threads and tangles of phospho-Tau protein. Intermediate AD: Severe deposit of neuropil threads of phospho-Tau protein. Advanced AD: Severe neuropil threads and tangles of phospho-Tau protein in the AON (All images are 20).,yes
PMC7888446,Figure_1,oa_package/2f/d6/PMC7888446.tar.gz,"['"" xml:lang=""en"" id=""mbt213692-fig-0001"" orientation=""portrait"" position=""float"">\nDictyostelium cells (A) and developmental time course (B, C, D) in the absence of nutrients.']","Fig. 1 cells (A) and developmental time course (B, C, D) in the absence of nutrients. Wildtype AX3 cells grown in shaking axenic culture were plated on glass (A), and on nonnutrient agar plates (B, C, D) and allowed to starve ( =0h) as previously described (Lacal ., ). (A) Image of AX3 cells plated on glass by digital interference contrast microscopy. In the absence of nutrients starvation is imminent, the amoebae stop dividing and activate several genes that will allow them to aggregate by chemotaxis towards cAMP diffusing from centrally located cells. Pictures of developing cells were taken during (B) aggregation ( =6h), (C) mound ( =12h) and (D) after completion of formation of fruiting bodies ( =24h) using timelapse phasecontrast microscopy. Scale bar in (A), 50m, whereas in (B, C, D) represents 1mm.",yes
PMC6763664,Figure_4,oa_package/1b/5f/PMC6763664.tar.gz,"['The immediate post-operative MRI demonstrated an almost complete reduction of the cystic lesion, with some sparse areas of contrast agent enhancement, and normalization in the lateral ventricle size [].', ':The immediate post-operative axial T1WI (a) demonstrated an almost complete reduction of the lesion, with some sparse areas of contrast agent enhancement (yellow arrows).']","Figure 4: The immediate post-operative axial T1WI (a) demonstrated an almost complete reduction of the lesion, with some sparse areas of contrast agent enhancement (yellow arrows). There is normalization in the lateral ventricle size. Coronal T2WI (b) shows post-surgical changes in the left frontal region (inside the yellow circle). There is hypointense material inside the ventricular system consistent with the degradation of hemoglobin (red arrows).",yes
PMC10654673,Figure_3,oa_package/bb/b4/PMC10654673.tar.gz,"['Of note, IL-6 secretion was compromised in TRAF6 knockout cells in WT and STINGgt/gt DCs (H), which is likely due to the additional role of TRAF6 downstream of CD40 signaling.', '.']","Figure 3. T cells induce a non-canonical STING-TRAF6-NF-B pathway in DCs (A) TRAF6 and STING protein expression following co-immunoprecipitation (coIP) of STING in WT BMDCs cultured with STING T cells and anti-CD3 for 1 or 3 h. BMDCs stimulated with DMXAA for 1 h were used as a control for canonical STING activation. (BE) Histograms and median fluorescence intensity (MFI) of phosphorylated IKK/ in BMDCs (B and C) and sDCs (D and E) after 1 h of T with or without anti-CD3. (F) Quantified %pro-IL-1+ WT or STING BMDCs with or withoutTRAF6 sgRNA transfection following culture with WT T cells in the presence of anti-CD3 for 3 h. (G and H) IL-1 or IL-6 was measured by ELISA in the supernatants of WT or STING BMDCs with or without TRAF6 sgRNA transfection cultured with WT T cells in the presence of anti-CD3. Error bars indicate SEM. In (A)(H), 3 independent experiments; in (B)(H), n = 58; two-way ANOVA.",yes
PMC7425722,Figure_8,oa_package/65/79/PMC7425722.tar.gz,"['These structures appeared to consist primarily of glial processes, although some individual cells were observed (', 'Although the processes at the confocal plane posterior to the ELM were extremely disorganized (Figs.', ' 8A, 8C, 8E, plane 1), there was a more organized pattern at the ELM focal plane (Figs.', ' 8B, 8D, 8F, plane 2).', '.', 'Interestingly, some PNA-positive structures were observed at the ELM focal plane internal to the membrane in the center of the atrophic area (see ']","Figure 8. The retina of donor 3, stained with GFAP ( ) and vimentin ( ) and imaged with the ELM en face is shown at higher magnification. ( ) Vimentin/GFAP-double-positive Mller cells have thick processes, which create a sharp border around the atrophic area ( ). Some individual cells are also observed ( ). ( ) In the focal plane of the ELM (13.8 m away), an intact ELM is observed to the right of the glial membrane border ( ). Rather than terminating and making junctions to create a honeycomb-like pattern, glial processes within the atrophic area extend along the retinal surface (i.e. lateral). Connections appear to exist between the Mller cell processes. ( ) Toward the center of the atrophic area, the Mller cells are very dense and appear disorganized. ( ) In the focal plane of the ELM, Mller cells are surrounding isolated PNA-positive surviving cone segments ( ). ( ) In the center of the Mller cell membrane external to the ELM, GFAP and vimentin-positive cells appear disorganized, creating a dense structure. ( ) At the ELM focal plane, Mller cell processes extend horizontally across the posterior retinal surface and having the appearance of fibroblastic scar ( ). indicates 50 m for all images.",yes
PMC8525303,Figure_1,oa_package/0e/52/PMC8525303.tar.gz,"[""References1von HerbayAIllesAWaldherrROttoHFPulmonary tumor thrombotic microangiopathy with pulmonary hypertensionCancer1990866(3)5879221637472UrugaHFujiiTKurosakiAHanadaSTakayaHMiyamotoAPulmonary tumor thrombotic microangiopathy: a clinical analysis of 30 autopsy casesIntern Med201352(12)131723237745403TanakaMTakahashiKKuriharaYYamamoto-RikitakeMOgusuSHirakawaHSignet ring cell carcinoma of unknown primary complicated with pulmonary tumor thrombotic microangiopathy and Krukenberg tumorCase Rep Oncol2018611(2)4127300575354YamauchiYNakanoKMiyagawaIInabaYNawataASatoNAn autopsy case of a patient with systemic sclerosis who developed marked pulmonary hypertension because of pulmonary tumor thrombotic microangiopathy caused by gastric carcinomaMod Rheumatol Case Rep202014(1)5662330869795WakabayashiYIwayaMAkitaMTakeuchiWYamazakiKIijimaAPulmonary tumor thrombotic microangiopathy caused by urothelial carcinoma expressing vascular endothelial growth factor, platelet-derived growth factor, and osteopontinIntern Med201655(6)6516269840856HigashiADohiYUraokaNSentaniKUgaSKinoshitaHThe potential role of inflammation associated with interaction between osteopontin and CD44 in a case of pulmonary tumor thrombotic microangiopathy caused by breast cancerIntern Med201554(22)287780265680027BannoAChibaKKasaiHNagamiKAnte-mortem diagnosis of pulmonary tumor thrombotic microangiopathy in a patient with unrecognized extramammary paget's diseaseBMJ Case Rep20168bcr20162166668OyamaYNishidaHKondoYkusabaTKadowakiHHaradaTPulmonary tumor thrombotic microangiopathy associated with extramammary paget's disease: an autopsy case reportPathol Int2020970(9)6805326384799OharaKFujisawaYYoshinoKKiyoharaYKadonoTMurataYA proposal for a TNM staging system for extramammary paget's disease: retrospective analysis of 301 patients with invasive primary tumorsJ Dermatol Sci2016983(3)23492732900710KatoHWatanabeSKariyaKNakamuraMMoritaAEfficacy of low-dose 5-fluorouracil/cisplatin therapy for invasive extramammary Paget's diseaseJ Dermatol2018545(5)56032944614811KatoHNakamuraMWatanabeSOdaTMoritaACombined serum carcinoembryonic antigen and cytokeratin 19 fragment levels provide a sensitive biomarker for lymph node metastasis in extramammary Paget's diseaseJ Dermatol2020747(7)76393241579912ImakuraTTezukaTInayamaMMiyamotoRAbeAOtsukaKA long-term survival case of pulmonary tumor thrombotic microangiopathy due to gastric cancer confirmed by the early diagnosis based on a transbronchial lung biopsyIntern Med202059(13)1621732612065a Preoperative skin findings: well-defined red to brown plaques are observed.""]","Fig. 1 Preoperative skin findings: well-defined red to brown plaques are observed. Pathologic findings of the primary lesion. Paget tumor cells are located in the epidermis. Computed tomography when dyspnea appeared. No pulmonary embolism is observed, and bilateral diffusely increased leaflet ground-glass-like absorption value is observed. Pulmonary blood flow scintigraphy. Defects scattered on the peripheral side of the lung field. Blood cytology (cell block, hematoxylin and eosin staining. 400) collected from the pulmonary artery. Atypical cells with nuclei of different sizes are observed. Blood cytology (cell block, cytokeratin 7. 400). Tumor cells are diffusely positive.",yes
PMC7500251,Figure_5,oa_package/d4/fa/PMC7500251.tar.gz,"[' 5a, b) [46, 51].', ' 5c) [46, 51].', 'Differential impact on neighboring tissues according the size of amyloid fibrils.', '5 m (b, c)Possible Impact of Nonfibrillar TTRAlthough the concept that amyloid fibrils themselves induce damage to surrounding tissues is widely accepted, recent studies suggest that nonfibrillar TTR also affects tissues in patients with ATTR amyloidosis [2].']","Fig. 5 Differential impact on neighboring tissues according the size of amyloid fibrils. Cross sections of sural nerve biopsy specimens from a patient with early-onset Val30Met ATTR with long and thick (i.e., large) amyloid fibrils ( , ) and a patient with non-Val30Met ATTR amyloidosis with short and thin (i.e., small) amyloid fibrils ( ). Uranyl acetate and lead citrate stain. Large amyloid fibrils seemed to pull surrounding tissues during their maturation, resulting in distortion and atrophy of Schwann cells, particularly those associated with small-diameter nerve fibers, such as unmyelinated fibers indicated by an arrow ( ). A high-powered view of the box in is shown in . Large myelinated fibers indicated by an asterisk are relatively preserved even though they are apposed to large amyloid fibrils. The influence of small amyloid fibrils on neighboring tissues seems to be less conspicuous than that of large amyloid fibrils ( ). An asterisk indicates an axon of an unmyelinated fiber. Scale bars 2m ( ) and 0.5m ( , )",yes
PMC11422786,Figure_5,oa_package/0f/06/PMC11422786.tar.gz,['A CT scan of the chest showing soft tissue density (red arrow) in the right lower bronchus.'],Figure 5 A CT scan of the chest showing soft tissue density (red arrow) in the right lower bronchus.,yes
PMC7594485,Figure_3,oa_package/cd/21/PMC7594485.tar.gz,"['The neoplastic\ncells labeled positive for CD18 antigen (b), consistent with a diagnosis of HS\n(Michigan State University Diagnostic Laboratory).', 'Immunohistochemistry for CD204\nwas also performed and was strongly positive, further confirming the histiocytic\norigin of this lesion (\n3c; Michigan State University Diagnostic Laboratory).', '(a) Final hematoxylin and eosin-stained sections of the splenic mass.', '1177_2055116920957196-fig3""/>Upon consultation with a medical oncologist, chemotherapy with CCNU (lomustine;\nWedgewood Pharmacy) was advised in conjunction with oral prednisolone and iron\ndextran injections.']","Figure 3 (a) Final hematoxylin and eosin-stained sections of the splenic mass. Themajority of the neoplastic cells demonstrate pleomorphism and a sheet-likearrangement with 30 bizarre mitotic figures per high-power field (greenarrow). Neoplastic cells are often seen containing phagocytized red bloodcells or hemosiderin (green arrowhead). . (b) Positive immunohistochemistry staining in tumortissues. Neoplastic cells label positive for CD18 antigen, confirmingleukocyte origin and supporting a diagnosis of histiocytic sarcoma (greenarrow). . (c) Showing strongly positivestaining immunohistochemistry for CD204 in tumor tissues. The neoplasticcells label positive for the CD204 antigen, confirming histiocyte origin(red arrow). There is also hemosiderin present (green arrowhead).",yes
PMC3317071,Figure_2,oa_package/e8/57/PMC3317071.tar.gz,"['Single-port laparoscopy revealed an obstructing lesion around the circumference of the bowel with mesenteric extension at this location (see ).', '001""/>(a) Obvious small bowel pathology seen at laparoscopy (in this case, histopathological of the excised specimen proved small bowel lymphoma).', '(b) The same loop of small bowel as shown in exteriorized via the single SALS incisions to allow formal wedge excision and reanastomosis to be performed extracorporeally.']","Figure 2 (a) Obvious small bowel pathology seen at laparoscopy (in this case, histopathological of the excised specimen proved small bowel lymphoma). (b) The same loop of small bowel as shown in exteriorized via the single SALS incisions to allow formal wedge excision and reanastomosis to be performed extracorporeally.",yes
PMC10532760,Figure_3,oa_package/70/5e/PMC10532760.tar.gz,[],"Figure 2 ( ) Immunohistochemistryantifibrinogen labeling presenting strong positive reaction bond to red blood cell mass (black arrow), 100; ( ) antiplatelets staining (CD61)strong positive reaction arranged in clumps or laminated pattern (black arrow), 100; ( ) antifibrin (ddimer) staining 50laminated disposition resembling to lines of Zahn (arrow); ( ) antiplatelet staining CD61, clumped platelets, 400; ( ) meshwork and rosettearranged strands of fibrin attached to the clumped platelets and nuclear fragments (black arrow); leukocytes (neutrophils are dominant) are entrapped in the fibrin meshwork, 400; ( ) fibrin staining in renal artery thromboembolism presenting strong positive reaction and diffuse, compact pattern (white arrow), 50.",yes
PMC11231514,Figure_1,oa_package/a0/92/PMC11231514.tar.gz,"['Imaging assessmentScreening mammography demonstrated new calcifications in the upper aspect of the right breast and in the upper outer quadrant of the left breasts ().', 'New calcifications on screening mammography.', 'Bilateral magnification views of calcifications.']",Fig. 1 New calcifications on screening mammography. Current screening mammogram (bottom row) demonstrates new calcifications in the upper aspect of the right breast (circle) and upper outer quadrant (arrowheads) when compared with prior mammograms (top row).,yes
PMC11063936,Figure_6,oa_package/a3/43/PMC11063936.tar.gz,"['0125, A and Supplemental A).', '54, B).', 'We next examined bulk RNA-Seq data from TCGA melanoma tumor samples (n = 473), and the relationship between the proliferative, invasive, Epgn1, and Epgn3 signatures with MITF and ITGA3 (C).', '06) (C).', 'To further substantiate the transcriptomic data in cell lines at the protein level, Western blotting for ITGA3 in a panel of primary and metastatic melanoma cells was performed, which confirmed the anticorrelation of ITGA3 with MITF (, D F, and Supplemental , B D).', 'Thus, both at transcript and protein levels, MITF-high cells showed low levels of ITGA3, whereas MITF-low cells expressed high ITGA3 levels (, A F, and Supplemental Table 1.', '7 cells showed a significant increase in tumor growth compared with mice injected with the NTC (G), indicating that loss of ITGA3 confers increased tumorigenesis.', 'SKmel147 luciferase expressing ITGA3 NTC versus KO cells were delivered via tail vein or intracardiac injection of immunocompromised mice (NOD/SCID/IL2 R / ) and analyzed by IVIS imaging, which showed increased seeding and metastatic burden to the lungs or the liver, respectively, in ITGA3-KO cells (, H and I).', 'It is expected that the findings are relevant for male mice (, H and I).', '7 cells (G), since the parental cell line was generated in male mice.', 'ITGA3 is overexpressed in Epgn3 high-risk melanomas and negatively regulates an invasive phenotype.']","Figure 6 ITGA3 is overexpressed in Epgn3 high-risk melanomas and negatively regulates an invasive phenotype. ( ) Heatmaps of proliferation, invasion, and pigment pathway genes and in the metastatic melanoma cell lines. ( ) Heatmaps of proliferation, invasion, and pigment pathway genes and in the CCLE melanoma cell lines. ( ) ssGSEA depicting correlations between proliferation, invasion, Epgn1, and Epgn3 gene signatures with and (upper panel). Dot plot of the expression for AJCC tumor stages, T0-T4 (TCGA melanoma data set, lower panel). ( ) Western blotting for ITGA3, MITF, and actin in the primary ( and ) and the metastatic melanoma cell lines ( ). ( ) Immunoblotting of ITGA3 and actin in the nontargeting control (NTC) and ITGA3-KO YUMMER1.7 cells. Tumor growth assay following the injection of the NTC and ITGA3-KO YUMMER1.7 cells in mice. = 0.05. ( and ) Immunoblotting of ITGA3 and actin in the NTC and ITGA3 KO SKmel147 luciferase cells ( ). Tail vein ( ) and intracardiac metastasis ( ) assays following the injection of the nontargeting control NTC and ITGA3 KO cells in mice, P = 4.33 10 and P = 0.0079, respectively. Mann Whitney test was used ( ).",yes
PMC6144732,Figure_1,oa_package/47/41/PMC6144732.tar.gz,"['3%) patients, respectively (Table I and ).', '0b013e318219ad3121716020.']","Figure 1. Radiological characteristics of GISTs. (A) Computed tomography scan (arterial phase): The lesion is indicated by the arrow. GIST of the stomach demonstrating a thickening of the gastric wall, with slight to moderate inhomogeneous enhancement following contrast agent injection. Lymph nodes around the mass exhibited reactive hyperplasia. (B) Magnetic resonance imaging (T2-weighted): The lesion is indicated by the arrow. GIST of the stomach sized ~97 cm and exhibiting heterogeneous hyperintense T2 signals. GIST, gastrointestinal stromal tumor.",yes
PMC7588822,Figure_2,oa_package/54/1f/PMC7588822.tar.gz,[],"FIGURE 2 Inhibitors of MEK1, VEGFR2, PDK1, PLK1, p38 MAPK, PI3K, and TrkA impair PV IgGinduced cell fragmentation in HaCaT keratinocytes. (a) HaCaT cell sheaths were exposed to defined mechanical stress in the presence of pemphigus vulgaris (PV) IgG. Normal human (NH) IgG served as a negative control. Inhibitors of MEK1 (selumetinib), VEGFR2 (vandetanib), PDK1 (BX795), PLK1 (rigosertib), p38 MAPK (BIRB 796), PI3K (A66), and TrkA (GW441756) impaired the PV IgGinduced fragmentation of the cell sheaths. The remaining 13 compounds (Figure ) had no effect on cell fragmentation. The PI3K inhibitor TGX221 is shown as one example of a compound that had no effect on PV IgGinduced cell fragmentation. All data were normalized to the fragmentation induced by PV IgG. Data are shown as individual values, with means SD from seven to nine independent replicates per group. * <0.05, significantly different, as indicated, from PV IgG; KruskalWallis H test followed by Dunn's post hoc test. (b) All compounds, with the exception of BX795, had no toxic effects at the indicated doses. Data are shown as individual values, with medians SD from three replicates per group and is an exploratory screening of the potential toxicity",yes
PMC5536906,Figure_5,oa_package/75/d1/PMC5536906.tar.gz,['.'],"Fig. 5. (A) In the CCA ligated group, T2 and TTC lesion volume were positively correlated ( =0.97, <0.0001), slope of linear regression=0.79. (B) In the CCA repaired group, T2 and TTC lesion volume were also positively correlated ( =0.75, =0.0012), slope of linear regression=0.89. Dotted lines show 95% confidence band of the best fit line.",yes
PMC2193459,Figure_4,oa_package/81/e8/PMC2193459.tar.gz,"['Unexpectedly, swelling of one or more of the large weight-bearing joints was observed in 60% of adult gp130 STAT/ STAT mice (n = 49, mean age 89 d ranging from 56 412 d), which resulted in flexion deformities of the hind limbs and reluctance of the mice to stand (, a and b).', 'Systematic clinical assessment showed that all gp130 STAT/ STAT mice showed stiffness of one or more limbs with restricted flexion and extension of the ankles as well as dorsiflexion at the wrist joints ( b to e).', 'Each limb of mice in cohorts of gp130 STAT/ STAT and gp130 STAT/wt animals was assessed twice per week for restriction in wrist and ankle movement as illustrated in c to f.', 'f3f""/>Clinical assessment of the joint disease in gp130 STAT/ STAT mice.']",Figure 4 Clinical assessment of the joint disease in gp130 mice. Swollen ankle joints and characteristic flexion deformities of hind legs in bilaterally affected gp130 mice (a). The restriction of ankle movement (b and c) and fixed flexion deformation of front paws (d and e) were consistent findings in gp130 mice but were never seen in gp130 littermates.,yes
PMC10366931,Figure_18,oa_package/b1/b3/PMC10366931.tar.gz,[],"Figure 18 Cholesterol granuloma. ( ) Axial fat-suppressed T2W and ( ) noncontrast T1W images show a lobulated, expansile lesion in the right petrous apex with intrinsic high signal intensity on T1 and mixed signal intensity with smooth hypointense margins on T2. The lesion is in close proximity with the petrous ICA (white arrow). ( ) Axial CISS image shows extension of the lesion into the right cerebellopontine angle with mild mass effect on the pons and abutment of the basilar artery (blue arrow). The lesion effaces right Dorellos canal, where there is mass effect with effacement of the cisternal segment of the right sixth cranial nerve (red arrow). ( ) Axial fat-saturated postcontrast T1-weighted image demonstrates no significant enhancement of the lesion, although this is difficult to evaluate due to intrinsic T1 hyperintensity.",yes
PMC10730449,Figure_13,oa_package/b5/cb/PMC10730449.tar.gz,[],"FIGURE 13 An 83-year-old female with gastrointestinal bleeding, haemorrhagic shock and renal dysfunction. Unfortunately, the patient expired within a few hours of imaging. The CT axial and sagittal images show a markedly flattened inferior vena cava (white thin arrows).",yes
PMC4606741,Figure_1,oa_package/e0/ea/PMC4606741.tar.gz,"['ResultsK18-P301L induced intracellular tau aggregationExogenous tau aggregates were prepared by using a truncated tau fragment, K18 (A).', 'ThS assay indicated that K18-P301L has higher propensity to form -sheet aggregates than K18-wt does (D).', 'To evaluate the prion-like activity of K18-wild type and K18-P301L, each of the pre-aggregates and aggregates were treated to the medium of tau-BiFC cells (C).', '5-fold increase respectively (F).', 'K18-wt, regardless of its aggregation status, did not induce tau BiFC fluorescence response up to 24 hrs (E).', 'To specify further the infectious forms of K18-P301L, we divided the aggregates into soluble and insoluble fractions by centrifugation (B).', 'The soluble and the insoluble fractions were evaluated by ThS assay (D).', '2-fold increase of tau BiFC fluorescence (F).', 'Evaluation of prion-like activity of tau aggregates.']","Figure 1 Evaluation of prion-like activity of tau aggregates. ( ) Tau40 is a full-length human tau and K18 is a microtubule-binding domain containing four repeated regions (R1-R4). K18-P301L contains a point mutation in the R2 region. ( ) A schematic diagram indicates preparation of tau aggregates ( ) Tau-BiFC fluorescence turns on only when tau assembles together. ( ) Thioflavin S (ThS) assay indicates the relative level of tau aggregation. Error bar represents S.D. of triplicate experiments. ( ) To identify prion-like tau aggregates, K18-wt or K18-P301L aggregates (10g/mL) were treated to Tau-BiFC cells for 24hrs. Then, Tau-BiFC cells were imaged by using Operetta High Content Imaging System. Scale bar=50m. ( ) The intensity of BiFC fluorescence was quantified by using Harmony software. Error bar indicates S.D. of triplicate experiments. Pre, pre-aggregates; Agg, aggregates; Sol, soluble; Ins, insoluble.",yes
PMC6837997,Figure_5,oa_package/04/e1/PMC6837997.tar.gz,"[', as previously published (31), although this occurred in lower amounts in mice infected with PRF sporozoites compared to mice that received the WT parasite (Supplementary ), as observed for total CD8+ T cells (A).', 'Cerebral myeloid populations were defined as CD45med/loCD11b+ cells, which excluded lymphocytes (A).', 'Based on their CD45 expression, myeloid cell populations were separated into CD45lo microglia and CD45med microglia, monocytes or macrophages, which are difficult to distinguish further (B).', 'Although it has been reported recently that non-ECM animals display lower numbers and less activation of microglia (32), we found that the CD45lo microglia number was not significantly different between na ve, WT sporozoite- and PRF sporozoite-infected animals (C).', 'There was a comparable increase in the number of CD45med microglia, monocytes and macrophages in both groups of infections (D).', 'Quantification of myeloid cells after infection.']","Figure 5 Quantification of myeloid cells after infection. Representative contour plots from day 8 after infection showing CD45 vs. CD11b expression on live cells obtained from nave mice (left), and mice infected with WT (center) and PRF (right) sporozoites. Indicated are gated populations of lymphocytes, CD45 CD11b cells (gray circles) and myeloid cells, CD45 CD11b cells (red circles). CD45 vs. CD11b expression on the Ly6G myeloid population. CD45 CD11b cells (orange circles) represent microglia and CD45 CD11b cells (green circles) activated microglia, monocytes and macrophages, respectively. Quantification of CD45 microglia. Shown are percentage (left) and absolute numbers (right) of CD45 Ly6G CD11b microglia. Quantification of CD45 Ly6G CD11b cells, which correspond to CD45 microglia, monocytes and macrophages. Shown are percentage (left) and absolute numbers (right) of CD45 microglia/monocytes/macrophages in the brain. The scatter dot plots in represent mean values ( ) from samples ( = 47) isolated 8 days after infection from two independent experiments. n.s, non-significant; < 0.05; < 0.01 (Mann-Whitney test). Representative images from the IBA-1 microglial cells of brain histological cross-sections. Cerebral vessels are indicated by arrows. The IBA-1 staining reaction was visualized with diaminobenzidine (DAB), highlighting some microglial cells with thin processes in nave mice, while they are more prominent in WT sporozoite-infected mice and they also tend to cluster around vessels. This feature is also pronounced in PRF sporozoite-infected mice, dark brown. Scale bar, 50 m.",yes
PMC11494294,Figure_2,oa_package/0e/bd/PMC11494294.tar.gz,"['Liver sections of uninfected mice showed normal hepatocytic architecture with no inflammatory cells in between or surrounding the central vein, normal hepatic lobules, and bile ducts (A).', 'mansoni caused marked granulomatous inflammation (B; black arrows).', 'mansoni-induced pathological alterations with marked reduction of granuloma size (F; black arrows).', 'Pre-infection with T.']","Figure 2 Pre-infection with reduced the size and number of granulomas in infected mice. Liver sections of different animal groups were stained with H&E stain. Representative photomicrograph of liver sections of uninfected mice showing preserved hepatic lobular architecture with small portal tracts (PT), central veins (CV), and intact hepatocytes (200x). Representative photomicrograph of liver sections of mice infected only with showing severe granulomatous inflammation around egg (black arrow) (100x). Representative photomicrographs of liver sections of mice infected only with showing thickened portal tracts with mononuclear cellular infiltration (blue arrows) severe granulomatous inflammation around egg (black arrow) (200x). Representative photomicrographs of liver sections of mice pre-infected with followed by showing markedly reduced granuloma size (black arrows) (100x). Representative photomicrographs of liver sections of mice pre-infected with followed by showing cellular egg granulomas (black arrows) with intact central ova with markedly reduced granuloma size (200x). Representative photomicrograph of liver sections of mice pre-infected with followed by showing mild granulomatous inflammation around egg (black arrow) (400x).",yes
PMC8659713,Figure_3,oa_package/98/8b/PMC8659713.tar.gz,"['(A).', '(B).', ', body weight decrease was largely similar in both groups (C).', ', resulting in significant differences in the clinical score (D 3F).', 'As described previously for the WT parasites, both WT and SBP-1 KO parasites show preference for reticulocytes and invade predominantly reticulocytes early during the infection (G and 3H) [Adipose tissue HO-1 upregulation increases anti-inflammatory adiponectin and mitochondrial fusion-associated proteins, while decreasing proinflammatory NOV and the mitochondrial fission-associated protein, FIS1.']","Figure 7 Adipose tissue HO-1 upregulation increases anti-inflammatory adiponectin and mitochondrial fusion-associated proteins, while decreasing proinflammatory NOV and the mitochondrial fission-associated protein, FIS1. (a) Representative western blots, and densitometry analysis of (b) NOV, (c) MFN1, (d) MFN2, (e) FIS1, and (f) adiponectin. (g) HO-1 mRNA levels in NOV overexpressing 3T3-L1 derived adipocytes. Results are mean SE, n=4, p<0.05 versus lean mice/control, #p<0.05 versus HF fed mice, and ##p<0.05 versus HF fed mice + CoPP.",yes
PMC6025801,Figure_6,oa_package/1f/57/PMC6025801.tar.gz,"[' 6a).', 'Identification of microglial proteins with increased expression in 5xFAD mouse microglia with relevance to human AD pathology.', '005Since App was identified as the most highly differentially expressed and human AD-relevant protein in 5xFAD microglia, we further investigated individual App peptide-level expression to determine whether this finding was driven by de-novo App synthesis or A phagocytosis by microglia.', ' 6b) of which 2 mapped to the A sequence, 2 mapped to the C-terminal YNPTY endocytosis motif and 1 mapped to N-term residues 439 450.', ' 6a) with increased expression in 5xFAD microglia and post-mortem human AD brain, Apoe and Cotl1 were also highly expressed at the transcriptional level by microglia [8, 29].']","Fig. 6 Identification of microglial proteins with increased expression in 5xFAD mouse microglia with relevance to human AD pathology. Scatter plot showing relative expression of proteins that were differentially expressed in both 5xFAD (vs WT) mouse microglia as well as a published human AD (vs non-AD non-disease control) whole brain proteomics dataset [ ]. Pearsons correlation coefficient was determined to assess overall concordance. The top 5 concordantly increased (red) and decreased (blue) proteins are highlighted. Proteins shown in red indicate increased expression while blue proteins indicate decreased expression in both datasets. Peptide-level analysis of all App peptides identified in the microglial proteome. Each unique peptide sequence is shown with a distinct color and each peptides expression across all 3 mouse groups (relative to global internal standard) is shown. * <0.05, ** <0.01, *** <0.005",yes
PMC7891317,Figure_5,oa_package/f7/cb/PMC7891317.tar.gz,"['STORM images of the same areas (C4, C5 and C6) highlight the distinct internal organization of the protein aggregatesColocalization and ultrastructural analysis of Lewy bodies and Lewy Neurites.']",Figure 5 Colocalization and ultrastructural analysis of Lewy bodies and Lewy Neurites. Conventional fluorescence microscopy image of a cortical Lewy body immunostained for p.syn and p.Tau. TwoColour STORM image of the same area revealing the internal architecture of the lesion and allowing to distinguish one protein from the other. Ultrastructural analysis of a cortical Lewy body using STORM (B2). Areas of the unstained cores and widths of p.syn branches were measured on superresolved images. Error bars indicate means with standard deviations. Conventional fluorescence microscopy image of a Lewy neurite (black arrowhead) immunostained for p.syn and neurofilaments (NF). TwoColour STORM image of the same area showing p.syn dense aggregates bounded by neurofilaments (enlarged in 3). Note the unaffected axon with normal calibre (white arrowhead),yes
PMC10355794,Figure_2,oa_package/c2/64/PMC10355794.tar.gz,"['Our cohort included 390 tumors diagnosed as GBM, 72 (18%) of which were histologically classified as another entity by DNA methylation (A).', '.', 'Ependymomas represented 6% of our cohort (109 cases).', 'DNA methylation reclassified 25 (23%) cases diagnosed as ependymoma while 9 tumors (8%) histologically considered ependymoma showed no matching DNA methylation class (B).', '(C).', 'DNA methylation analysis found that 15% of tumors histologically diagnosed as oligodendroglioma were reclassified as a different tumor (N = 10) (D).', 'Our study included 117 (6%) cases of medulloblastoma, the most common malignant brain tumor of children, 110 (94%) of which were concordant and accurately classified into 4 prognostically relevant subgroups (F),.', '18,19 IDH mutant astrocytomas accounted for 5% of our cohort (N = 96) and DNA methylation modified the diagnosis in 31% of cases (N = 29) (E).', 'DNA methylation, upgraded histologic astrocytoma IDH mutant WHO grade 2 to the DNA methylation class high-grade IDH mutated astrocytoma in 13% of cases, and downgraded astrocytoma IDH mutant WHO grade 3 and 4 to a low-grade IDH astrocytoma in 43% and 28% of cases, respectively (E).', '2 In our cohort, all 75 tumors classified as ependymoma were successfully subclassified by DNA methylation into established prognostic subgroups (B).']","Figure 2. Diagnostic utility for accurate diagnosis and prognostic stratification. Six tumor groups with the highest yield of DNA methylation included GBM, ependymoma, glioneuronal tumors, oligodendroglioma, astrocytoma IDH mutant, and medulloblastoma. GBM ( =390) were a complete match in 82% of cases, a diagnostic mismatch in 13% of cases, and did not classify with any entity by DNA methylation in 5% of cases (no match). Most misdiagnosed GBMs were reclassified as diffuse midline glioma K27 altered (31%), anaplastic pilocytic astrocytoma (10%), and pleomorphic xanthoastrocytoma (7%). Ependymoma (N=109) had a complete match rate of 69%, a diagnostic mismatch rate of 23%, and a no-match rate of 8%. Ependymomas were most commonly reclassified as myxopapillary ependymoma (24%) and subependymoma (28%), Glio-neuronal tumors ( =160) had a diagnostic complete match rate of 61%, a diagnostic mismatch rate of 27%, and a no match rate of 12%. Pilocytic astrocytoma ( =80) were reclassified by DNA methylation in 20 cases (28%) and DNA methylation upgraded the diagnosis in 11% of these cases. Ganglioglioma ( =32) had a diagnostic mismatch rate of 31% and DNA methylation upgraded the diagnosis in 15% of cases. Oligodendroglioma ( =66) had a complete match rate of 83%, a diagnostic mismatch rate of 15%, and a no-match rate of 2%. Tumors diagnosed histologically as oligodendroglioma are most often reclassified as astrocytoma (10%), glioblastoma (2%), and DNET (3%). Astrocytoma IDH mutant ( =96) were a complete match in 65% of cases, a diagnostic mismatch in 31% of cases, and a no match in 4% of cases. Astrocytoma IDH mutant World Health Organization (WHO) grade 2 was most reclassified as a higher-grade IDH mutant astrocytoma (11%), astrocytoma IDH mutant WHO grade 3 was most commonly reclassified as a lower-grade IDH mutant glioma in 48% of cases, and astrocytoma IDH mutant WHO grade 4 most commonly reclassified as a lower grade IDH mutant astrocytoma in 28% of cases. While medulloblastoma is rarely misdiagnosed (3% of cases) DNA methylation provides prognostic information by stratifying tumors into established molecular subgroups including Shh, Wnt, group 3, and group 4.",yes
PMC3621222,Figure_4,oa_package/82/82/PMC3621222.tar.gz,[],Figure 4 Levoposition of the heart,yes
PMC4890939,Figure_5,oa_package/da/4a/PMC4890939.tar.gz,"[' 5).', 'Antibiotic resistance profiles (%) among isolated Pseudomonas strainsOn the other hand, international data demonstrate variable resistance: one study revealed strains isolated from chronic wounds resistant to piperacillin/tazobactam and aztreonam [18], another study found P.']",Fig. 5 Antibiotic resistance profiles (%) among isolated strains,yes
PMC5746521,Figure_1,oa_package/4e/5b/PMC5746521.tar.gz,"['Use of T2A sequences to express galactosidase, luciferase and human chorionic gonadotropin (hCG) from the endogenous Hmox1 promoter\nA, schematic diagram of the engineering strategy used to create the Hmox1 multireporter allele.', '1APAP induces reporter activity in the liver that is mitigated by the antidote NACTriplicate reporter mice were treated with vehicle, 300 mg kg 1 APAP, 300 mg kg 1 NAC, or a combination of 300 mg kg 1 APAP + 300 mg kg 1 NAC, and necropsied 24 h later.', '2Ionizing radiation does not alter bioluminescence in reporter miceTriplicate reporter mice were sham irradiated (0 Gy) or exposed to 4 Gy of IR.', '3Ionizing radiation induces p21 in liver and LI, even at low dosesTriplicate p21 reporter mice were sham irradiated or exposed to 1 , 2 , or 4 Gy of IR.']","Figure 1 Use of T2A sequences to express galactosidase, luciferase and human chorionic gonadotropin (hCG) from the endogenous promoter , schematic diagram of the engineering strategy used to create the multireporter allele. See text for further details. , triplicate WT (+/+), heterozygous reporter (+/r), or homozygous reporter (r/r) mice were treated with 30mgkg haemin and killed 18h later. All mice were male and aged between 12 and 13weeks. , pooled liver, lung, or large intestine (LI) whole lysates were prepared and blotted for Hmox1, galactosidase and firefly luciferase. GAPDH was used as a loading control. Molecular weights are noted on the right. The asterisk indicates the slowermigrating 2Atagged form of Hmox1. , levels of hepatic and mRNA relative to 18s rRNA. Data are presented as arbitrary units and have been transformed so that the median level of mRNA in WT mice is set to a value of 1. The median level of mRNA in heterozygous reporter mice was set to 1. , bioluminescence images of mice before and after treatment with haemin (hemin). Note that one of the homozygous reporter mice (asterisked) displayed an atypically low luminescence posthaemin. Data points from this mouse are highlighted in graphs , and by increased transparency. , quantification of images in . , firefly luciferase activity per mg protein was measured in lysates prepared from lung, liver, or LI. Data are presented in arbitrary units. In order to highlight the fold difference in activity between heterozygous and homozygous reporter mice, data have been transformed so that the median activities in organs from heterozygous reporter mice are set to a value of 1.",yes
PMC8023077,Figure_3,oa_package/97/af/PMC8023077.tar.gz,[],Figure3. T2 images on sagittal and axial plane that show flow void in the left cavernous body.,yes
PMC4260288,Figure_1,oa_package/c1/82/PMC4260288.tar.gz,"['6 mm, hypoechotic mass about 8 cm in the lower uterine segment with necrotic change and increased vascularity with low resistance was seen around it [].', 'Sonography view of hypoechotic mass in the uterusUterus with lower segment massHistopathology examination demonstrates trophoblastic invasion into the myometriumCase 2A 32-year-old gravid 3, para 2 woman with a history of one prior cesarean section was referred to the Department of Obstetrics and Gynecology because of continuous vaginal bleeding from 3 weeks ago.']",Figure 1 Sonography view of hypoechotic mass in the uterus,yes
PMC8516024,Figure_3,oa_package/8c/3d/PMC8516024.tar.gz,['Giant Touton-like histiocytes comprised of multinucleated cells.'],Figure 3 Giant Touton-like histiocytes comprised of multinucleated cells.,yes
PMC11283088,Figure_2,oa_package/4c/a5/PMC11283088.tar.gz,"[""Comprising four distinct primary domains as shown in , the tau protein's destiny is sculpted by alternative splicing, particularly within the N-terminal projection region and the microtubule-binding domain (MBD)."", 'The structure and isoforms of tau.', 'Tau stabilizes microtubules by binding and promoting microtubule assembly [22].']","Fig. 1 Tau pathology and nanotechnology applications (a) Pathology of Alzheimer's disease in a healthy brain, tau proteins stabilize the microtubules, which are responsible for keeping the structure and function of nerve cells. In AD, tau proteins become abnormal and begin to accumulate in the brain. This accumulation results in the formation of NFTs. (b) The application of nanotechnology in treating tau pathology in Alzheimer's disease includes regulating tau phosphorylation, inhibiting tau aggregation and diffusion, stabilizing microtubules, and promoting tau clearance. Created with .",yes
PMC10667107,Figure_12,oa_package/69/93/PMC10667107.tar.gz,[],"Extended Data Fig. 6 Microglia density and their levels of identity markers do not change in HD mice and striatal astrocytes do not engulf a greater amount of synaptic material. Confocal images of microglia in the dorsal striatum of 7 mo zQ175 mice and WT littermates co-stained with antibodies to Iba1 and putative microglia identity markers and TMEM119. Scale bar = 50m Bar charts show the % of and TMEM119 immunoreactive area above a set threshold (determined using an algorithm developed by Costes and Lockett ) that colocalizes with the area of Iba1 staining for the images in , for and Iba1 n=4 WT and 5 zQ175 mice (2F and 2M for WT and 3F and 2M for zQ175); for TMEM119 and Iba1 n=3 WT and 4 zQ175 mice (2F and 1M for WT and 2M and 2F for zQ175). Unpaired two-tailed t-test for comparison of WT and zQ175 mice, for and Iba1 p=0.819 and for TMEM119 and Iba1 p=0.968. Bar charts show the Pearsons correlation coefficient for Iba1 and and Iba1 and TMEM119 for the images shown in , for and Iba1 n=4 WT and 5 zQ175 mice (2F and 2M for WT and 3F and 2M for zQ175); for TMEM119 and Iba1 n=3 WT and 4 zQ175 mice (2F and 1M for WT and 2M and 2F for zQ175). Unpaired t test for comparison of WT and zQ175 mice and Iba1 p=0.113 and for TMEM119 and Iba1 p=0.736. Bar chart shows quantification of microglial cell density in the dorsal striatum of 4 mo zQ175 mice and WT littermates, n=4 WT and 4 zQ175 mice (2F and 2M for both genotypes). Unpaired two-tailed t-test p=0.610. Bar chart shows quantification of microglial cell density in the striatum of 7 mo Q175mice and WT littermates, n=4 WT and 5 zQ175 mice (2F and 2M for WT and 3F and 2M for zQ175). Unpaired two-tailed t-test p=0.263 Bar chart shows the level of transcripts in striatal extracts from 7 mo zQ175 mice and WT littermates, n=3 WT and 3 zQ175 mice (3F and 3M for both genotypes). Unpaired two-tailed t-test p=0.135. Bar chart shows the level of Iba1 transcripts in striatal extracts from 7 mo zQ175 and mice and WT littermates, n=3 WT and 3 zQ175 mice (3F and 3M for both genotypes). Unpaired two-tailed t-test p=0.141. Representative orthogonal view of an Iba1 stained microglia in the dorsal striatum of a 4 mo zQ175 mice that had previously received a motor cortex injection of pAAV2-hsyn-EGFP at P1/2. Scale bar = 10m This experiment was repeated 6 times. Representative orthogonal view of an Iba1 stained microglia in the dorsal striatum of a 4 mo zQ175 Homer-GFP mouse. Scale bar = 10m. This experiment was repeated 5 times. Representative orthogonal view of an S100 stained astrocyte in the dorsal striatum of a 4 mo zQ175 Homer-GFP mouse. Scale bar = 10m. This experiment was repeated four times. Representative surface rendered images of S100 stained astrocytes (red) and engulfed Homer-GFP inputs (green) in the dorsal striatum of 4 mo zQ175 Homer-GFP mice and WT Homer-GFP littermates. Scale bar = 10m. Bar chart shows quantification of the relative % astrocyte engulfment of Homer-GFP (the volume of engulfed Homer-GFP expressed as a percentage of the total volume of the astrocyte) in 4 mo zQ175 Homer-GFP mice relative to that seen in WT Homer-GFP littermate controls, n=6 WT Homer-GFP and 4 zQ175 Homer-GFP mice (3F and 3M for WT Homer-GFP and 2F and 2M for zQ175 Homer-GFP mice). Unpaired two-tailed t-test, p=0.485. For bar charts, bars depict the mean. All error bars represent SEM. Stars depict level of significance with *=p<0.05, **p=<0.01 and ***p<0.001.",yes
PMC9667791,Figure_4,oa_package/f6/19/PMC9667791.tar.gz,"['073) (A).', '"" rowspan=""1"" colspan=""1"">5The cohort was further stratified by tumour site within the PSC cohort (iCCA or pCCA) (B).']",FIGURE 4 Kaplan-Meier curve detailing the survival of patients with PSC all the other indications for LT. Kaplan-Meier curve detailing the survival of patients with PSC grouped by site (iCCA v pCCA). Logrank test.,yes
PMC3226467,Figure_3,oa_package/f9/f2/PMC3226467.tar.gz,"['In HeLa cells, the ECL1 site is both core and complex glycosylated and trafficked to the cell surface in CFTR containing or lacking the natural ECL4 sites (B).', 'Core and complex glycosylation of the ECL1 site containing proteins was verified by electrophoretic mobility shifts after treatment with specific glycosidases (C).', 'Of importance, the introduced ECL1 site is efficiently core glycosylated with no nonglycosylated band detected, suggesting that this variant is efficiently integrated into the ER membrane (, B and C, A, and Supplemental S2).', 'FIGURE 4:Experimental TM1 ER luminal integration profile edges for WT and CF mutant CFTR.', 'By contrast, the original TM1 prediction used as a starting point in development of the ECL1 site indicated the TM1 ER luminal boundary was at residue 102 (A).', 'Thus both the experimentally identified TM2 integration profile edge and the original predicted TM2 boundary are at residue 118 (A), as are many of the other predictions ().']","FIGURE 3: An artificial glycosylation site introduced in ECL1 between TM1 and TM2 is glycosylated and trafficked to the cell surface. (A) Schematic of the predicted TM1 and TM2 ER luminal boundaries (large circles) with the introduced artificial glycosylation site. Residues are shown with CFTR residues as small circles and introduced residues as small squares. Twelve residues are designed to be between the core glycosylation site (NST) and the predicted TM boundaries, which are shown as large circles. (B) Cellular trafficking of CFTR containing the natural ECL4 glycosylation sites and/or the artificial ECL1 site in HeLa cells was analyzed by Western blot analysis. Top, total cell CFTR; middle, cell surface CFTR identified by biotinylation; actin is a control. (C) Core and complex glycosylation of CFTR containing the ECL1 site in the presence or absence of the ECL4 glycosylation sites was verified by digestion with glycosidase selective for core and complex glycosylation (PNGaseF) or core glycosylation (Endo H). The positions of CFTR with no glycosylation (band A), core (band B), and complex glycosylation (band C) are marked.",yes
PMC4135345,Figure_4,oa_package/0e/47/PMC4135345.tar.gz,['Ectopic expression of a CGG90 repeat results in Purkinje cell loss.'],"Figure 4 Cerebellum of control mouse without a CGG90 repeat (that is, L7 ) showing normal distribution of Purkinje cells in the Purkinje cell layer. Higher magnification of the Purkinje cell layer in control mouse. Selective Purkinje cell loss in 32-week-old mouse expressing a CGG90 repeat under the L7 Purkinje cell-specific promoter (that is, L7CGG90 ). Purkinje cell loss in shown at higher magnification in L7CGG90 mouse. gl, granule cell layer; ml, molecular layer; pcl, Purkinje cell layer. (Adapted from [ ]).",yes
PMC4994244,Figure_8,oa_package/e8/57/PMC4994244.tar.gz,"[' 8).', ' 8a, d) nor in fractions of striatal tissue containing the terminals of transduced nigral neurons (', ' 8b, e).', ' 8a).', ' 8d).', ' 8b, e).', ' 8c, f).', 'Assessment of S129 phosphorylated human alpha-synuclein in rat brain tissue.', '01; two-tailed Mann-Whitney test; Error bars indicate SEMAlterations in phosphorylation state of human alpha-synuclein (h-asyn) in Parkinson s disease (PD) patients with and without dementiaFirst, we set out to evaluate the sample reproducibility for the AlphaLISA duplexing assay.']","Fig. 8 Assessment of S129 phosphorylated human alpha-synuclein in rat brain tissue. Quantification of pS129 h-asyn species after overexpression from rAAV6 vectors in the substantia nigra ( , , , ) or striatum ( , ). Tissue pieces ( =6 per group) were processed sequentially with 1% Triton (about 300ng for striatal and 90ng for VM samples of protein loaded) ( ) followed by 1% SDS (about 600ng of protein loaded) ( ) containing lysis buffer. Total (open) and pS129 (filled) h-asyn levels are presented in overlapping bars. Numbers inside the bars show the percent of pS129 to total h-asyn. ** <0.01; two-tailed Mann-Whitney test; indicate SEM",yes
PMC11533988,Figure_2,oa_package/c1/30/PMC11533988.tar.gz,"['Ongoing studies in the field of spatial transcriptomics are poised to provide invaluable working foundations using carefully characterised patient data (figure 2).', 'Advancements in biomarkers and molecular imaging are crucial in this field and are likely to revolutionise our understanding of the disease by providing insight into its dynamic progression in the same patient (or animal) over time (figure 2).']","FIGURE 1 Continued. d) Lungs from a patient with PH associated with heart failure with preserved ejection fraction (group 2) showing lung oedema, hemosiderin-laden macrophages (HM) and important muscularisation of PV and venules that may reach the degree of arterialisation on PVs. e) In PH associated with idiopathic pulmonary fibrosis (IPF), lung parenchyma shows extensive collagen-rich fibrosis, destruction of the elastic fibres and distortion of the alveoloseptal architecture (upper image; collagen is orange, elastic fibres are black;Elasticavan Giesonsaffron staining). Smooth muscle actin staining highlights the increase in interstitial smooth muscle cells and myofibroblasts, as well as heavily remodelled PA in this area (lower left image); note that interstitial and PA remodelling are often associated in IPF. Staining with collagen 1A1 highlights typical fibroblastic foci in this same area, which are frequently located in the interstitial space of small airways and considered to be the activity hotspots of IPF. Note their vicinity to remodelled PA. f) Lungs from chronic thromboembolic pulmonary hypertension (CTEPH) patients typically show PA with thrombotic lesions that are partially organised/recanalised (upper image). PV may show remodelling, too, which is probably due to functional bronchopulmonary anastomoses and increase in venous drainage of systemic blood from bronchial arteries and vasa vasorum, in analogy to PAH (see earlier). Of note, pre- and post-capillary microvessels (arterioles and venules, lower image) show secondary remodelling that is not due to embolism or thrombosis, but to increase of shear stress and pressure. g) An example of PH group 5 (sarcoidosis-associated PH (SAPH)). Several mechanisms are responsible for the increase of pulmonary vascular resistance in this condition, including hypoxaemia, interstitial fibrosis, vascular wall remodelling resembling PAH and PVOD, but also compression of pulmonary vessels by chronic granulomatous inflammation, as shown. Note at least three granulomas with epithelioid histiocytes and Langhans giant cells () surrounding the PA, which displays medial thickening and near-occlusive intimal fibrosis. The lower image depicts an endothelial staining focusing on a PV visibly compressed by granulomas. All photographed cases were collected from different institutions and with courtesy of Anton Vonk-Nordegraaf (University of Amsterdam, Amsterdam, the Netherlands), Marc Humbert (University Paris-Saclay, Le Kremlin-Bicetre, France) and Werner Seeger (University of Giessen, Giessen, Germany), and their pathology departments. LHD: left heart disease; CLD: chronic lung disease.",yes
PMC10449997,Figure_7,oa_package/8f/66/PMC10449997.tar.gz,"[' 7).', ' 7).', 'Co-transfection strategy to characterize the dominant phenotype of TRPC6 mutants.', 'Scale bar 100 mCo-transfection strategy to characterize the dominant phenotype of TRPC6 mutants.']","Fig. 7 Co-transfection strategy to characterize the dominant phenotype of TRPC6 mutants. Cells were transfected with mixtures of TRPC6 Wild Type (WT) C-terminally fused to YFP and mCherry, G757D C-terminally fused to YFP and P112Q fused to mCherry (mCh+). Representative flow cytometry plots show the gating strategy to sort double-positive cells expressing both YFP and mCherry (YFP+mCh+, top right) for each co-transfected cells and the remaining 3 gates to sort cells expressing only mChr+(Top left), YFP (bottom right), and non-transfected cells (bottom left). Microscopic images of ( ) sorted cells expressing both WT-YFP/P112Q-mCh represented in combined image as YFP+mCh+, ( ) sorted cells expressing both G757D-YFP/WT-mCh represented in combined image as YFP+mCh+, ( ) sorted cells expressing both G757D-YFP/P112Q-mCh represented in combined image as YFP+mCh+. Scale bar 100m",yes
PMC4161480,Figure_2,oa_package/ef/0a/PMC4161480.tar.gz,"['001) was practically abolished in the absence of a functional SPI1 system ( invC) (A C).', 'In accordance with these results, immunostaining (D), plating of isolated epithelial cells after gentamicin-treatment to remove extracellular Salmonella (', '2E F) and flow cytometry (G) detected enterocytes infected by WT but not SPI1-deficient Salmonella.', 'The reduced total small intestinal organ counts at later points after low dose infection may result from the lack of intraepithelial bacteria (E F).', 'g002Comparative analysis of WT and invasion-deficient Salmonella.', 'g002""/>Requirement for enterocyte invasion in the absence of M cells in the neonate intestineIn contrast to the critical role of SPI1 in neonate mice, intestinal colonization but also spread to spleen, liver and MLN in adult streptomycin-pretreated animals was largely SPI1-independent (H and Increased Synthesis and Secretion of DPPC in Ig-Hepta / MiceMacrophages seem to be functioning normally and taking up surfactants as much as possible, as evidenced by their large and foamy structure (D F), but the amount of the surfactants secreted exceeds the phagocytic ability of macrophages.']",10.1371/journal.pone.0069451.g006,yes
PMC3701681,Figure_2,oa_package/c6/60/PMC3701681.tar.gz,"['All bacteria-treated groups demonstrated increased CD11c+ MHC II+ dendritic cell frequencies in the PP as compared to the medium treated mice (A).', 'salivarius UCC118 administration (B).', 'Although no effect of probiotic treatment was observed on % CD80+ DCs in the PP (C), the treatment with L.', 'lactis MG1363 did increase the activation status of the dendritic cells in the PP as demonstrated by increased frequencies of CD86+ DCs (D).', 'g002Effects of three bacterial strains on Peyer s Patch dendritic cells.']",10.1371/journal.pone.0068952.g002,yes
PMC10435320,Figure_1,oa_package/cc/b7/PMC10435320.tar.gz,[],"Figure1 Hepatic cavernous hemangioma and changes. taken in January 2022, shows the radiological presentation of a large hepatic cavernous hemangioma on the right lobe. taken in June 2022 after switching from Camrelizumab to Tislelizumab, demonstrates the changes observed, with a significant reduction and near disappearance of the hepatic cavernous hemangioma.",yes
PMC5709977,Figure_5,oa_package/34/b0/PMC5709977.tar.gz,"[' 5a) in fAD, sAD, and control neural cultures at different time points of TD.', ' 5b, Additional file 6: S3a).', ' 5c, Additional file 6: S3b).', ' 5d, Additional file 6: S3c).', ' 5e and f, Additional file 6: S3d and e).', 'Western blot analysis of total TAU and phosphorylated TAU (pTAU) protein.', '05)\nGSK3B activation in AD-derived neuronsScientific evidence suggests that GSK3B is involved in many pathological hallmarks of AD, including hyperphosphorylation of TAU [58], increased A production [59], memory impairment, and neuronal loss [60].', ' 5a): Ser262, Ser202/Thr205, Ser396, Ser400/Thr403/Ser404, Thr181, and Thr231 in both fAD and sAD neurons.']","Fig. 5 Western blot analysis of total TAU and phosphorylated TAU (pTAU) protein. Schematic representation of human TAU isoform (441 amino acids) with the functional projection and microtubule-binding domains. Projection domains including a proline-rich region and N-terminal part interact with cytoskeletal elements and are involved in signal transduction. Microtubule-binding domains with a C-terminal part regulate the microtubule polymerization and bind to proteins such as presenilin 1 (PSEN1). Epitopes of pTAU antibodies analyzed in this study are indicated on the scheme. Densitometric analysis of TAU phosphorylated at different epitopes: S262, S396, S202/T205, T181, and S400/T403/S404. All samples were analyzed at day 70 of terminal differentiation. The amount of pTAU relative to total TAU levels in the lysates was measured. as the loading control was used to normalize the data. All values are the meanSEM ( =3). Dunnetts test was performed to evaluate the significance of groups compared with control (* <0.05)",yes
PMC6754864,Figure_1,oa_package/03/06/PMC6754864.tar.gz,"['Four hours after induction, the patient was noted to have developed marked swelling in the chest, neck, and face, particularly in the periorbital region ().', 'Massive subcutaneous emphysema and hypercarbia: complications of carbon dioxide absorption during extraperitoneal and intraperitoneal laparoscopic surgery-case studiesAANA Journal200270645646112526151Massive subcutaneous emphysema with swelling of the face and periorbital area.']",Figure 1 Massive subcutaneous emphysema with swelling of the face and periorbital area. The emphysema was caused by laparoscopic robotic myectomy.,yes
PMC8808045,Figure_5,oa_package/1f/7b/PMC8808045.tar.gz,"['Typical dermoscope pictures of SK before and directly after HIFU treatment are shown in .', 'Typical dermoscope appearance of seborrheic keratosis.']",Figure 5 Typical dermoscope appearance of seborrheic keratosis. ( ) Before HIFU treatment. ( ) Directly after treatment. Whitening of the epidermis and denaturation of the superficial skin structure as a reaction to the thermal and mechanical effects of HIFU can be observed.,yes
PMC7568066,Figure_1,oa_package/37/06/PMC7568066.tar.gz,"['Kidney parenchyma was obliterated by an extensive mixed lymphoid infiltrate composed of small cells, spindled cells, and a predominance of large lymphoid cells with abnormal nuclei (A).', 'The infiltrating cells were diffusely positive for B-cell markers CD20 (B), PAX5 (Paired Box 5), and CD79a, characteristic of a large B-cell lymphoma.', 'There was also positivity for Bcl6 (C) and negativity for CD10 and MUM1 (Multiple Myeloma 1), indicating germinal center phenotype by the Hans classification.', '6 The Ki-67 proliferative rate was 70% to 80% (D).', ' 1An extensive infiltrate obliterated the kidney parenchyma (A, hematoxylin and eosin; original magnification, 50), composed of a mixture of small lymphoid-appearing cells, some spindly cells, and cells with enlarged atypical nuclei (B, hematoxylin and eosin; original magnification, 200; inset; original magnification, 400).']","Figure1 An extensive infiltrate obliterated the kidney parenchyma (A, hematoxylin and eosin; original magnification,50), composed of a mixture of small lymphoid-appearing cells, some spindly cells, and cells with enlarged atypical nuclei (B, hematoxylin and eosin; original magnification,200; inset; original magnification, 400). Atypical cells were diffusely positive for CD20 (C) and Bcl6 (D, top of the picture) with 70% to 80% Ki-67 positivity (D, bottom of the picture; original magnification,10; insets; original magnification, 40).",yes
PMC6698274,Figure_1,oa_package/17/00/PMC6698274.tar.gz,"['Exploration revealed a huge cyst with dimensions of 24 cm 20 cm 16 cm, arising from the retroperitoneum, projects into transverse mesocolon and displacing loops of the small intestine (, ).', 'Per op findings of cyst (case 1).', '']",Fig. 1 Per op findings of cyst (case 1).,yes
PMC9397543,Figure_3,oa_package/ac/3a/PMC9397543.tar.gz,"['PI - pneumatosis intestinalisCT of the abdomen without contrast, supine position, displaying PI (black arrows), diffuse ascites (white arrowheads), and PI (black arrows), atrophy of intestinal villi (pinheads), and pneumoperitoneum (white arrows).']","Figure 3 CT of the abdomen without contrast, supine position, displaying PI (black arrows), diffuse ascites (white arrowheads), and PI (black arrows), atrophy of intestinal villi (pinheads), and pneumoperitoneum (white arrows). PI - pneumatosis intestinalis",yes
PMC9720051,Figure_2,oa_package/93/fc/PMC9720051.tar.gz,['MRI of the pelvis showing thickened endometrium measuring 17 mm with intermediate signals on axial and sagittal T2WI (a and c).'],"Figure 2 MRI of the pelvis showing thickened endometrium measuring 17 mm with intermediate signals on axial and sagittal T2WI (a and c). Minimal myometrial invasion (<50%) is seen on sagittal T2WI (c) with disruption of the junctional zone. Minimally enhancing endometrial tissue (b). No pelvic lymphadenopathy. MRI was staged as T1a N0. MRI: magnetic resonance imaging, T2WI: T2-weighted imaging",yes
PMC7059022,Figure_1,oa_package/23/63/PMC7059022.tar.gz,"[' shows FCM images with the corresponding image of H E-stained tissue section of CNB obtained from bone that was correctly recognized by both pathologists.', 'Additional FCM and corresponding H E-stained images of 6 optimal and 3 nondiagnostic and suboptimal cases of CNBs obtained from different sites that were correctly recognized by both pathologists are shown as e and e in the Supplement.', '.', 'e.']",Figure 1. Images of Core-Needle Biopsy Specimen Obtained From Bone Grayscale and digitally pseudocolorized fluorescence confocal microscopy images of interventional radiologyguided core-needle biopsy specimens and corresponding hematoxylin-eosinstained tissue section from bone that were accurately diagnosed by both study pathologists independently using fluorescence confocal microscopy. The fluorescence confocal microscopy images resemble the hematoxylin-eosinstained images and demonstrate the presence of poorly differentiated carcinoma. Scale bar indicates 1500 m in A and B and 100 m in C and D. Original magnification 100 in E.,yes
PMC8857170,Figure_1,oa_package/db/0a/PMC8857170.tar.gz,"[' 1A), which paralleled the accumulation of LBs.', ' 1B).', ' 1A, C).', '\nAccumulation of p62 aggregates over time in malinKO mice.', '0041p62 Is Essential for LB Formation in Muscle and Heart Tissue But Not In the BrainTo evaluate the impact of p62 deletion on LB formation and LD progression, we generated malinKO mice devoid of p62 (malinKO + p62KO).', 'pdf"">Supplementary file1 (PDF 3492 KB)Supplementary .']","Fig. 1 Accumulation of p62 aggregates over time in malin mice. Representative images of the progressive accumulation of p62 in the cortex and hippocampus of malin mice. DAPI: 4 ',6-diamidino-2-fenilindol; WGA: wheat germ agglutinin.Scale bar: 50m. Quantification of the number of p62-positiveLBs per area (n of particles/mm ) in the prefrontal cortex or hippocampus (B) and skeletal muscle (C). =712 mice. For comparisons between groups, one-way ANOVA was performed using Prism7 software (GraphPad). * <0.05, <0.01, <0.001, <0.0001. : control 4m vs. control 11m =0.9997; control 4m vs. malin 4m =0.7191; control 4m vs. malin 11m =0.0010; control 11m vs. malin 4m =0.7829; control 11m vs. malin 11m =0.0013; and malin 4m vs. malin 11m =0.0001. : control 4m vs. control 11m >0.9999; control 4m vs. malin 4m =0.9929; control 4m vs. malin 11m =0.0109; control 11m vs. malin 4m =0.9936; control 11m vs. malin 11m =0.0111; and malin 4m vs. malin 11m =0.0005. : control 4m vs. control 11m =0.9556; control 4m vs. malin 4m =0.5429; control 4m vs. malin 11m =0.0020; control 11m vs. malin 4m =0.7784; control 11m vs. malin 11m =0.0014; and malin 4m vs. malin 11m =0.0041",yes
PMC8507512,Figure_2,oa_package/02/d0/PMC8507512.tar.gz,"[' 2 would be in line with such assumptions and data presented by Faucheux et al.', 'Detection of iron deposits (Fe III) using Berlin blue reaction: intracytoplasmic fine granules in neuromelanin containing neurons of SN both in controls (A, B) and in PD cases (D F) as well in glial cells in controls (C) (thin arrows).', 'G-I Iron-free vessels in controls, J-L iron deposits in vessels of PD brainsPathological events triggering Parkinson s diseaseConclusions from this part areIRON increases with age.']","Fig. 2 Detection of iron deposits (Fe III) using Berlin blue reaction: intracytoplasmic fine granules in neuromelanin containing neurons of SN both in controls ( , ) and in PD cases ( ) as well in glial cells in controls ( ) (thin arrows). Coarsely deposits in capillary walls in PD cases (e.g. ) (thick arrow), whereas in controls ( ) such reaction is missing (200magnification, scale bar 50m). Iron-free vessels in controls, iron deposits in vessels of PD brains",yes
PMC8715831,Figure_4,oa_package/97/81/PMC8715831.tar.gz,[],"FIGURE 4 . (A) Chemical classification of identified metabolites in mouse caecum provided by HMDB ( ). A total of 256 compounds (91%) are categorized into nine super classes. (B) The score scatter plot of 282 caecal metabolites by principal component analysis (PCA). Most variation was imposed by PC1 (30.8%) and PC2 (12.3%). * <0.05 as compared with WD. (C) Overview of the metabolic features. Pie charts present the number of metabolites that were significantly different in other groups, respectively compared to WD (Student's test, <0.05). Red and blue colours present significantly higher or lower abundance in other groups, respectively compared to WD ( <0.05). The network is constructed based on chemical structural similarity (Tanimoto score) and KEGG reaction pair (substrateproduct relation), which results in distinctive metabolic modules indicated by box. Red and blue colours present significantly higher or lower abundant in NC, LL, and PP groups, respectively compared to WD (Student's t test, <0.05). Node sizes are determined by the ratios. HMDB, human metabolome database; KEGG, Kyoto encyclopedia of genes and genomes; LL, ; NC, normal control; PC1, Climatic index 1; PC2, Climatic index 2; PP, WD, Western diet",yes
PMC9586657,Figure_3,oa_package/7d/f8/PMC9586657.tar.gz,"['Likewise, in L02 cells, ISG15 and HERC5 expressions were elevated (A-E) and -catenin was decreased ( C, F) at the protein level.', '\n.', 'The changes in levels of SREBP-1, PPAR- (G-H), and TG (I) were consistent with the AML-12 cells and the previously described EtOH-fed mouse liver tissues.']",Figure 3. ISG15 and HERC5 levels were increased and -catenin levels were decreased in EtOH-stimulated L02 cells. (A-B) The expression levels of ISG15 and HERC5 were detected by qRT-PCR. (C-E) The expression levels of ISG15 and HERC5 were detected by western blotting. (F-G) The expression levels of -Catenin were detected by qRT-PCR and western blotting. (H) The expression levels of SREBP-1 and PPAR- were detected by qRT-PCR. (I) The expression levels of SREBP-1 and PPAR- were detected by western blotting. (J) The expression of TG levels in cell supernatants. versus control group. Data represent the meanSD for 34 independent experiments.,yes
PMC6891921,Figure_5,oa_package/bc/9a/PMC6891921.tar.gz,['Ang Tie2 Vegf signaling largely regulates microvessel morphology.'],"Figure 5 AngTie2Vegf signaling largely regulates microvessel morphology. a) Comparison of parameters between triculture microvessels treated with various inhibitors. Shown is mean s.e.m. b) Comparison of control and Tie2 inhibited live microvessels. ECs are shown in red and HPPs in green. HLFs are unlabeled. Scale bar is 200 m. c) Representative histograms showing expression of Tie2 on ECs and HPPs. d) Ang1 and Ang2 expression across individual samples and e) from pooled (> = 5) samples. Significance is indicated by * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001, ***** < 0.00001, with oneway ANOVA and Tukey test.",yes
PMC8376382,Figure_3,oa_package/5d/e8/PMC8376382.tar.gz,['Results of gastroscopy (descending duodenum).'],Figure 3 Results of gastroscopy (descending duodenum). A large number of mature mature adipose tissues are seen in the submucosa.,yes
PMC6679414,Figure_3,oa_package/10/35/PMC6679414.tar.gz,['.'],"Fig. 3. (A) Light microscopy of retinal semi-thin sections of B6/J +/+, +/N and N/N mice aged 1821months. OS, outer segments; IS, inner segments; ONL, outer nuclear layer; OPL, outer plexiform layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars: 20m. (B) Ultra-thin sections showing the RPE layer with associated POS; red arrows (inset), laminar infoldings; blue arrows, cytoplasmic vesicles. Scale bars: 5m. BM, Bruchs membrane. (C) Quantification of RPE thickness in +/+, +/N and N/N mice. Retinae were divided in ten sections anterior and posterior of the optic nerve head (ONH), ending at the ora serrata (OS). The means.d. is given for each sample; =25 mice per genotype, two retinal sections were analyzed per mouse. (D) Quantitative real-time expression of genes involved in apoptosis and/or the activation of microglia and macrophages. , Annexin 1; , Caspase 1; , Caspase 8; , C-C motif chemokine ligand 2; , chemokine C-C motif ligand 6; , CD68 antigen. Expression was normalized to Hprt1 (hypoxanthine phosphoribosyltransferase 1). RNA was extracted from the RPE/retina complex isolated from eyes of two B6/J +/+ and three +/N and N/N mice, respectively (each animal 1213months of age). The means.d. is given for each sample. All samples were performed in triplicates. Also, see results for genes , , and in . (E) Measurements of DHA levels were performed by GC-mass spectrometry (MS) of retinal lysates from 1012month-old CD-1 +/+, +/N and N/N mice. =812 mice per genotype (F) Levels of total A2E, atRALdi-PE and A2-DHP-PE as a function of age were examined by HPLC-analysis in 9- and 20-month-old B6/J +/+, +/N and N/N mice. Data points represent values from six pooled eye cups per time point and genotype.",yes
PMC11328004,Figure_399,oa_package/e2/f1/PMC11328004.tar.gz,[],"Figure 75 Real-time tracking of necrosis of HeLa cells prestainedwith complex (15 M, 4 h). = 405 nm, = 500540nm (green luminescence) and 590630 nm (red luminescence).Adapted with permission from Ref ( ). Copyright 2022 Wiley-VCH.",yes
PMC9553857,Figure_6,oa_package/16/64/PMC9553857.tar.gz,['Multiple pleural metastases (arrows) were noted in a 60-year-old woman with type B2 thymoma.'],"Figure 6 Multiple pleural metastases (arrows) were noted in a 60-year-old woman with type B2 thymoma. Pleural lesions were ipsilateral to the primary tumor (star). A pleural plaque (blue arrow) had lower ADC than the primary tumor (0.5 10 and 1.11 10 mm /sec, respectively).",yes
PMC7463502,Figure_1,oa_package/ca/50/PMC7463502.tar.gz,"['At the age of 20 days (without the manifestations of AD-like pathology), the expression of six genes (Camk2b, Gadd45g, Map3k5, Mapk14, Mapk8ip2, and Mapkapk3) was higher and Map3k7 gene expression was lower in OXYS rats than in Wistar rats (a).', 'Among the genes with increased expression, we noted Calm3, Ccm2, Dlk1, Gadd45g, Irf1, Map3k10, Mapk14, and Traf2, and five genes (Dusp10, Dusp16, Map3k1, Spag9, and Tab2) were downregulated (a).', '3390/antiox806017731208023The impact of age on the number of differentially expressed genes (DEGs) involved in the p38 mitogen-activated protein kinase signaling pathway (MAPKsp) in OXYS rats (a).']",Figure 1 The impact of age on the number of differentially expressed genes (DEGs) involved in the p38 mitogen-activated protein kinase signaling pathway (MAPKsp) in OXYS rats ( ). The Venn diagram shows overlapping sets of DEGs among 20-day-old and 5- and 18-month-old OXYS rats compared to age-matched Wistar rats ( ).,yes
PMC3407004,Figure_2,oa_package/6d/1a/PMC3407004.tar.gz,['LPS but not intraplantar capsaicin results in significant changes in spinal tight junction protein occludin.'],"Figure 2 Intrathecal LPS injection ( ) but not intraplantar capsaicin ( ) induces disturbed occludin morphology in lumbar spinal cord. Number of intact vessels are significantly decreased in ipsilateral dorsal, ipsilateral ventral, contralateral dorsal, and contralateral ventral spinal cord quadrants after intrathecal LPS, but not at various timepoints after capsaicin in either male ( ) or female ( rats. intrathecal (IT), lipopolysaccharide (LPS), von Willebrand Factor (VWF), occludin (OCC) ipsilateral dorsal (ID), ipsilateral ventral (IV), contralateral dorsal (CD), contralateral ventral (CV), others as in Figure .",yes
PMC6283105,Figure_4,oa_package/2d/f8/PMC6283105.tar.gz,['Low power view of tumor showing moderately differentiated squamous cell carcinoma cells (hemotoxylin and eosin 100).'],Figure 4 Low power view of tumor showing moderately differentiated squamous cell carcinoma cells (hemotoxylin and eosin 100).,yes
PMC5362896,Figure_5,oa_package/88/17/PMC5362896.tar.gz,"['Abdominal radiographs confirmed left renomegaly (), and on thoracic radiographs the mass in the dorsal aspect of the left caudal lung lobe remained the same and had not increased in size.', 'Abdominal radiographs.', '1177_2055116916659516-fig5""/>Due to lack of evidence supporting an infectious etiology, the results obtained on the initial fine-needle aspiration of the pulmonary mass, and the strong suspicion of a neoplasm, it was decided to perform vitreocentesis, ultrasound-guided aspiration of the left kidney and repeat the aspiration of the pulmonary mass.']",Figure 5 Abdominal radiographs. (a) Ventrodorsal and (b) lateral radiographic views of the abdomen taken on day 22 following initial presentation. Enlargement of the left kidney was noted (arrows). The mass in the dorsal aspect of the left caudal lung lobe remained the same and had not increased in size,yes
PMC9498659,Figure_7,oa_package/d6/38/PMC9498659.tar.gz,"['The moderate clinodactyly of the 5th finger and the shortening of 4 5 fingers as well as 4 5 metacarpal and metatarsal bones led to the characteristic picture of brachymetaphalangism and were noticed in all the patients of this group (misdiagnosed as Albright osteodystrophy in one of the patients) ().', 'Photos and radiographs of the hands of the patients with different variants in the SLC26A2 gene.']","Figure 7 Photos and radiographs of the hands of the patients with different variants in the gene. ( ) Patient with the homozygous c.1957T > A (p.Cys653Ser) variant. ( ) Patient with the compound heterozygous variant, c.1957T > A (p.Cys653Ser) and c.26 + 2T > C. ( ) Patient with the compound heterozygous variant, c.835C > T (p.Arg279Trp) and c.26 + 2T > C.",yes
PMC7666369,Figure_5,oa_package/50/d8/PMC7666369.tar.gz,"[':The patient underwent a lumpectomy of the left breast, including a resection of the left breast cancer as well as a portion of the underlying pectoralis major given its proximity to the cancer.']",Fig. 5 CT axial (D) and sagittal (E) images of the chest show the left breast mass with calcifications of dense bone density.,yes
PMC9472137,Figure_4,oa_package/44/7e/PMC9472137.tar.gz,"['By special von Kossa stains, rare Michaelis-Gutmann bodies were identified as well as many eosinophilic globules (figure 4).', 'Von Kossa-positive Michaelis-Gutmann bodies (von Kossa, 400 ).']","Figure 4 Von Kossa-positive Michaelis-Gutmann bodies (von Kossa, 400).",yes
PMC8626479,Figure_5,oa_package/04/9f/PMC8626479.tar.gz,"[' 5A.', ' 5B; Kruskal Wallis test p 0.', ' 5C).', ' 5D; Kruskal Wallis test p 0.', 'TBI and A 42 protofibril exposure cause mitochondrial damage in astrocytes.', 'Upregulated autophagy upon A 42 protofibril exposure or TBI, but not following the combined exposureAutophagy, the conserved pathway for the elimination of aberrant proteins and organelles, such as damaged mitochondria, has been linked to neurodegenerative diseases and TBI24,38 40.']","Figure 5 TBI and A protofibril exposure cause mitochondrial damage in astrocytes. ( )The health of mitochondria in astrocytes (GFAP) in response to the TBI and A protofibril exposure was investigated. Representative images show mitochondria labeled with CellLight Mitochondria-GFP and cell nuclei with DAPI. The sites of the injury are indicated by the dashed lines. ( )Mitochondrial network fragmentation was assessed and quantified. While control cells possess a predominately healthy-looking mitochondrial network with strands of branching mitochondria, exposed cells display various states of mitochondrial network disruption. Increasing fragmentation can be observed and at late stages defective mitochondrial membranes causes the release of labeled mitochondrial proteins that become visible in the cytosol. Fifteen images per independent cell culture (n=3) and experimental group were analyzed and reported. ( )Representative TEM images showing examples of mitochondria observed in control cells and cells undergone TBI, A protofibril exposure or the combination of both. Abnormal fusion events were primarily observed in exposed cells and some mitochondria possessed marked lesions. ( ) Quantification of the aspect ratio (mitochondrial length/width) confirmed these observations. Five cells per experimental group were analyzed; quantifying the mitochondria in a total of 5568 TEM images per group. Statistical analyses were performed with the KruskalWallis test followed by Dunns multiple comparisons test, *p0.05 **p0.01 ***p0.001. Scale bars 50m( ) and 20m ( ).",yes
PMC10725308,Figure_7,oa_package/37/42/PMC10725308.tar.gz,[],Figure7. 15_CD8_IM showing the deviation from the central alignment.,yes
PMC5996523,Figure_2,oa_package/b3/40/PMC5996523.tar.gz,"['2, 3).', '2CT scan showing pulmonary right upper lobe noduleCT scan showing pulmonary right lower lobe nodule']",Fig. 2 CT scan showing pulmonary right upper lobe nodule,yes
PMC11233802,Figure_2,oa_package/1a/9e/PMC11233802.tar.gz,['Each identified feature was annotated with the aid of binary masks (see ).'],"FIGURE 2 Annotation methodology for histology patches (top row) depicting features associated with a blood vessel regeneration (replacement of a biodegradable polymer by formed vascular tissue). Histological annotations delineated with segmentation masks (bottom row) include arteriole lumen (red), arteriole media (pink), arteriole adventitia (light pink), venule lumen (blue), venule wall (light blue), capillary lumen (brown), capillary wall (tan), immune cells (lime), and nerve trunks (yellow).",yes
PMC2823048,Figure_1,oa_package/c6/0a/PMC2823048.tar.gz,"['The posteroanterior and lateral chest radiograph revealed a round mass lesion in the left anterior chest ().', 'European Musculo-Skeletal Oncology SocietyActa Orthop Scand1999703533601056926515GibbsJFHuangPPLeeRJMcGrathBBrooksJMcKinleyBMalignant fibrous histiocytoma: an institutional reviewCancer Invest200119232711291552The posteroanterior (A) and lateral (B) chest radiograph revealed a round mass lesion (arrows) in the left anterior chest.']",Fig. 1 The posteroanterior (A) and lateral (B) chest radiograph revealed a round mass lesion (arrows) in the left anterior chest.,yes
PMC3725171,Figure_3,oa_package/be/b8/PMC3725171.tar.gz,['Coronal contrast enhanced computed tomography images demonstrate ruptured hydatid lesion within right liver lobe with perihepatic free fluid.'],Figure 3 Coronal contrast enhanced computed tomography images demonstrate ruptured hydatid lesion within right liver lobe with perihepatic free fluid.,yes
PMC5036965,Figure_15,oa_package/18/61/PMC5036965.tar.gz,[],10.7554/eLife.17047.021,yes
PMC3524565,Figure_5,oa_package/88/cb/PMC3524565.tar.gz,"['5 (A).', '5 +/c and c/c embryos, and expression was limited to hypertrophic chondrocytes (B, arrows).', 'Tgcog expression was not detected in +/+ controls (B).', 'Tgcog expression did not alter the localization of collagen X in +/c and c/c compared with +/+ embryos (C).', '5 (D).', '5 +/+, +/c, and c/c embryos (E).', '.', '1369_0022155412458436-fig5""/>E15.', '5 embryos ().', '5 in the HZ of the developing growth plate (B,C), demonstrating that the ColXTg\ncog transgene, with its relatively long Col10a1 promoter, recapitulated the expression of the endogenous Col10a1 gene.', '5 was not sufficient to provoke a UPR in terms of the upregulation of BiP expression (D) in contrast to the m/m Col10a1 p.']","Figure 5. Histological and in situ characterization of E14.5 embryonic growth plates. (A) Hematoxylin and eosin (H&E) staining on sections from the tibial growth plate of wild type (+/+), hemizygous (+/c), and homozygous (c/c) embryos for the transgene. (B) Immunohistochemistry (IHC) for Tg using a myc antibody on sections from the embryonic tibial growth plate of +/+, +/c, and c/c embryos. The dark purple staining indicates the intracellular accumulation of Tg protein in the hypertrophic zone (HZ) of +/c and c/c embryos (indicated by the arrows). (C) IHC for collagen X on sections from the tibial growth plates of +/+, +/c, and c/c. The dark purple staining indicates collagen X localization within the extracellular matrix. The HZ is indicated by the vertical lines. (D) In situ hybridization (ISH) for mRNA on sections from the tibial growth plate of +/+, +/c, and c/c embryos. Levels of mRNA expression were below the level of detection at E14.5 in all embryos. (E) IHC for BiP on sections from the tibial growth plate of +/+, +/c, and c/c embryos. Levels of accumulated BiP protein were below the level of detection at E14.5 in all embryos. Scale bar = 100 m.",yes
PMC2727051,Figure_1,oa_package/18/df/PMC2727051.tar.gz,"['To create an understanding of cell-type specific changes as SLE progresses, we profiled the cytokine response of multiple major immune cell subsets (A) in two genotypes: lpr as the disease model and MRL as control.', '(B) Stat2 and Stat4 phosphorylation was not measured due to their limited activation by most cytokines in our profile.', 'Examples of signaling responses that showed progressive changes over the time course are shown (C).', 'g001Experimental design for profiling changes in the murine immune signaling network during SLE progression.', 'In the absence of exogenous stimulation there was no detectable activation of Stat3 or Stat5 over the disease course, though there was low but detectable increase in staining for pStat1 and pStat6 that was larger in B cells than in T cells (C, 2B, C, D and CT AnalysisCT scans were retrospectively and independently read by two chest radiologists (J.']","Figure 2: Example of indeterminate CT imaging features for COVID-19 in a 36-year-old female patient. Chest CT image shows bilateral multifocal ground-glass opacities (arrows), which were mainly located in the right upper lobe. There was no posterior part/lower lobe predilection, and there was also no peripheral/subpleural distribution of lung abnormalities.",yes
PMC9431694,Figure_4,oa_package/a5/70/PMC9431694.tar.gz,"['Brain imaging of Anxa6gfp mice using anti-GFP antibodies detected annexin A6GFP protein, largely restricted to the plasma membrane, and this was well seen in cortical neurons, as marked by NeuN positivity (, A and B).', 'As neuron maturation progressed from day 4 to day 10 in culture, annexin A6GFP levels significantly increased (C; see complete unedited blots in the supplemental material).', 'Genomically encoded annexin A6GFP localized to the site of neuron injury, forming a repair cap visible within 1 2 seconds of injury, which persisted through the 60 seconds of imaging (D and Supplemental Video 3).', 'Annexin A6GFP localizes at the site of neuron membrane injury.']","Figure 4 Annexin A6GFP localizes at the site of neuron membrane injury. ( ) Anti-GFP (shown in green, indicated by arrows) antibody detects genomically encoded annexin A6GFP protein in adult cortex and midbrain but not in WT mice. DAPI (blue) marks nuclei. Anti-NeuN (red) marks mature neurons. ( ) Anti-GFP (green, arrow) antibody, which detects genomically encoded annexin A6GFP protein, localized to the peripheral membrane of NeuN cortical neurons as visualized with high-magnification confocal imaging. ( ) Embryonic neurons were isolated from mice. Genomically encoded annexin A6GFP expression increases with maturation. After 10 days, neurons expressed 2-fold more annexin A6GFP than at 4 days. ( ) Isolated neurons were injured with a confocal laser. Genomically encoded annexin A6GFP (green) quickly localized into a repair cap (white arrow) visible 4 seconds postinjury. Multiple cells from = 3 mice. * < 0.05 by 1-way ANOVA.",yes
PMC11315576,Figure_3,oa_package/8d/c7/PMC11315576.tar.gz,['.'],Figure 3. Immunohistochemistry for cervical and breast cancer-related pathology. (A) Cytokeratin 7 (B) Gata binding protein 3 (C) Grosscystic disease fluid protein-15 (D) Mammaglobin.,yes
PMC8425173,Figure_4,oa_package/33/d1/PMC8425173.tar.gz,"[' 4 shows the distribution for each case.', 'In the case in which the patch labels are true (', 'In the case in which the patch labels are random (', 'FIGURE 4.']",FIGURE4. Prediction ability of nonrandom (blue) and random (red) classifier. (A) 224 224: histogram of the number of patches derived from non-EoE images vs. the probability that they will be classified as active EoE by the nonrandom classifier. (B) 224 224: histogram of the number of patches derived from active EoE images vs. the probability that they will be classified as active EoE by the nonrandom classifier. (C) Random 224 224: histogram of the number of patches derived from non-EoE-labeled images vs. the probability that they will be classified as active EoE by the random classifier. (D) Random 224 224: histogram of the number of patches derived from active EoE-labeled images vs. the probability that they will be classified as active EoE by the random classifier.,yes
PMC9104584,Figure_3,oa_package/8b/b5/PMC9104584.tar.gz,"['Based on developmental and morphological studies, it has been postulated that abnormalities in each of the above steps of the outflow tract development process lead to the following mechanisms of congenital heart disease (): (1) double outlet right ventricle (DORV): impaired leftward migration of conotruncus and/or abnormal persistence/absorption of subarterial conus, (2) persistent truncus arteriosus (PTA): insufficient formation of the conotruncal septum, (3) transposition of the great arteries (TGA): insufficient twisting of the conotruncal septum and/or abnormal persistence/absorption of subarterial conus, and (4) tetralogy of Fallot (TOF): hypoplasia of subpulmonic conus and/or malalignment of aorta and the left ventricle12).', 'Normal development and congenital defects of the cardiac outflow tract.']","Figure 3 Normal development and congenital defects of the cardiac outflow tract. Based on developmental and morphological studies, abnormalities in each step of the outflow tract development (upper figures) lead to the spectrum of congenital heart disease involving the outflow tract (lower figures). Bold arrow represents leftward movement of conotruncus (CT). RV: right ventricle, LV: left ventricle, IVS: interventricular septum, Ao: aorta, PA: pulmonary artery, DORV: double outlet right ventricle, PTA: persistent truncus arteriosus, TGA: transposition of the great arteries, and TOF: tetralogy of Fallot.",yes
PMC7750402,Figure_7,oa_package/81/9a/PMC7750402.tar.gz,['Within the operation an intralesional curettage and defect reconstruction with bone substitute were performed.'],Fig. 7 Within the operation an intralesional curettage and defect reconstruction with bone substitute were performed.,yes
PMC9312477,Figure_2,oa_package/6c/f5/PMC9312477.tar.gz,"['Collectively, these results indicate that IFN- production by dermal pDCs is a key element in the early phase of psoriatic skin lesion induction [18] ().', ', who demonstrated that TNF- regulates IFN- production (), and blocking endogenous TNF- maintained IFN- production by pDCs [51].', 'Pathogenesis of psoriasis.']","Figure 2 Pathogenesis of psoriasis. The characteristic TNF-IL-23-Th17 pathway in psoriasis is illustrated. In the early phase of psoriatic skin lesion, pDC-IFN pathway plays a role. TNF- inhibits IFN- production by pDCs. pDC, plasmacytoid DC; cDC, conventional DC.",yes
PMC2777255,Figure_9,oa_package/f7/55/PMC2777255.tar.gz,[],"F Myelin-enriched fraction can be isolated from myelinating cultures and used for myelin protein kinetic studies. (A) The data shown indicate the average protein yield (28.9 2.2 g) of the myelin fraction obtained from three experimental repeats for myelinating cultures (MC) as compared with the yield (6.7 0.9 g) from the myelin-like fraction isolated from oligodendrocyte cultures (OC). Each preparation was from one litter of five to seven embryos or five or six pups. Each tissue source yielded sufficient material to generate 6 35-mm Petri dish cultures. (B) The average protein recovered from the myelin fraction for each preparation is expressed relative to the total protein content of the homogenate. (C) Representative examples of western blot analysis of the two myelin proteins proteolipid protein (PLP)DM20 and myelin-associated glycoprotein (MAG) detected in the total homogenate and myelin fraction from each culture type. The total homogenate (T) and the myelin fractions (M) were loaded onto the gel at a ratio of 20 : 1 (typically 20 g and 1 g respectively). Western blot staining for neuronal proteins (phosphorylated neurofilament) showed that there was no detectable contamination of the myelin fraction harvested from the myelinating cultures. It also showed that a significant proportion of the protein in the total cell homogenate of myelinating cultures is neuronally derived. The blots were stripped and reprobed with -actin to assess equivalence of loading. (D) The intensity of the protein in the myelin fraction was corrected for the amount loaded onto the gel and expressed as the fold enrichment relative to the total homogenate intensity. (E) Nascent PLP and DM20 isoforms were incorporated into the myelin fraction. Representative phosphorimages of nascent PLP and DM20 immunoprecipitated from the myelin fraction and total homogenate are shown for each culture type. The nitrocellulose membranes were analysed by western blotting to compare the levels of PLP and DM20 isoforms recovered from each fraction. (F) The western blots were then quantitatively analysed by assessing the intensity of the signal for nascent PLP and DM20 isoforms relative to the total cellular homogenate. Note that the quantification is of the actual signal intensities in these blots, which were derived from loading 20 times more total cell homogenate then myelin fraction. Thus, if this 20-fold factor is taken into account, the absolute yield of PLP and DM20 in the myelin fraction is much higher than in the total cell homogenate.",yes
PMC9732680,Figure_4,oa_package/d3/89/PMC9732680.tar.gz,"['pTDP 43 aggregates identified in lymph node parenchyma, endothelial cells, and chondrocytes long before symptom onset of ALS.']","Figure 4 pTDP43 aggregates identified in lymph node parenchyma, endothelial cells, and chondrocytes long before symptom onset of ALS. (A) H&E (left) and pTDP43 (right), low power (top) and high power (bottom) images of an active lymph node germinal centre with evidence of cells containing pTDP43 aggregates (white arrows) within the mantle zone rim of the lymphoid follicle. (B) Low power (left) and high power (right) images of the paranodal tissue demonstrating blood vessel pTDP43 aggregation (white arrows) in adjacent feeder vessels. (C) H&E (left) and pTDP43 (right) images showing no evidence of pTDP43 aggregates in a muscle biopsy taken at point of diagnosis from patient 1.",yes
PMC5117226,Figure_4,oa_package/78/bc/PMC5117226.tar.gz,['.'],"Fig. 4. (A) AMPK activity is not altered in PD lymphoblasts. Each PD ( =30) and control ( =8) cell line was assayed in duplicate in each of at least two independent experiments and means were calculated. AMPK activities in the PD cells were not elevated significantly (single-sided Welch -test). Quantitative western blots also revealed no significant change in the ratio of activated AMPK (phosphorylated 1 subunit) to total AMPK in PD cells ( ). (B) Mitochondrial mass is not elevated in PD lymphoblasts. Mitochondrial mass was measured in lymphoblasts from PD and control individuals using MitoTracker Green fluorescence. Each PD ( =30) and control ( =9) cell line was assayed in at least three independent experiments and means were calculated. The fluorescence in the PD lines was not significantly elevated (single-sided Welch test). (C) Mitochondrial genome copy number is unchanged in PD lymphoblasts. Relative mitochondrial genome copy number was measured in duplicate in semi-quantitative RT-PCR as the difference in qPCR threshold cycle number between the mitochondrial genes encoding ND1 and ND4 (mitochondrially encoded subunits of complex I) and the nuclear gene encoding 2-microglobulin. Each PD ( =30) and control ( =8) cell line was assayed in two or three independent experiments and means were calculated. The mitochondrial genome number was not significantly different in the PD and control cell lines (single-sided Welch tests). Very similar results were obtained using the nuclear 18S rDNA genes as the genome loading control (not shown). (D) Mitochondrial gene expression is elevated in PD lymphoblasts. Quantitative reverse transcription PCR was used to assay ND1 and ND4 mRNAs (mitochondrially encoded subunits of complex I) relative to the nuclear-encoded cytoplasmic 18S rRNA (which provided the internal loading control). Each PD ( =26) and control ( =7) cell line was assayed in duplicate in one to five independent experiments and mean threshold cycle numbers (C ) were calculated. For all cell lines the differences between mRNA expression levels for both subunits and the corresponding averages for the control cell lines were determined. These were found to be elevated significantly in the PD cells (single-sided Welch test). Similar results were found in a subset of samples using the 2-microglobulin mRNA as the internal control (not shown). (E,F) Steady-state levels of OXPHOS proteins are elevated in PD lymphoblasts. (E) Crude lymphoblast protein extracts were separated on SDS-PAGE and western blotted using commercial antibodies. The blot shown is an example with fluorescence signals as shown for the ATP5A and SDHB bands, normalized to the corresponding -tubulin signal in the same track and expressed relative to the average control values. (F) Background-subtracted fluorescence signals for each band were normalized against the background-subtracted -tubulin signal in the same track and expressed relative to the average control value. The ATP5A and SDHB levels were significantly higher in PD cells ( =11 PD and =6 controls, single-sided Welch test). Similar results were found in experiments with 19 PD samples and four control samples using an anti--actin antibody to detect actin as the internal loading control ( ). Error bars are s.e.m.",yes
PMC7690745,Figure_10,oa_package/15/c7/PMC7690745.tar.gz,[],"Figure 10 Immunohistochemically stained sections of mouse brains; regions of interest (ROI) in cortical and hippocampal areas are marked in whole brain images (100 magnification) and depicted separately for further semi-quantification of amyloid plaques; 35 weeks of age, 30 weeks on experimental diet. ( ) Exemplary section of a C57BL/6J wild type animal. ( ) Exemplary section of an knock-in animal.",yes
PMC5664416,Figure_2,oa_package/40/18/PMC5664416.tar.gz,"[' 2 provides an overview on the hypothetic action of CD8+ T cells, TH1 and TH17 cells in defense against rickettsiae.', '', 'Inhibition of either TNF or IL-17A in this situation renders TH17 cells as protective as TH1 cells (c)\nT cell-mediated cross-protection and T cell antigensCross-protective immune response between different rickettsial species has been described for animals as well as for humans.']","Fig.2 Mechanisms of T cell-mediated protection. CD8 T cells differentiate in the infection with rickettsiae to cytotoxic T cells that induce apoptosis in infected cells via the release of perforin and granzymes. In addition, CD8 T cells produce IFN and TNF. These cytokines induce the production of NO by M and other cells and, thus, enable bacterial killing. The release of cytokines by CD8 T cells is sufficient for protection at least in the infection with some rickettsial species such as where the cytotoxic activity is not essential for bacterial defense ( ). CD4 T cells usually differentiate into T 1 cells that produce IFN and TNF in the infection with rickettsiae. T 1 cells are protective by the induction of NO and bacterial killing by M and other cells ( ). In the absence of IFN, CD4 T cells develop into T 17 cells in the infection with rickettsiae. These cells release IL-17A, IL-22 and TNF. IL-17A and TNF synergistically induce the production of NO und ROS by M and other cells. In addition, these cytokines induce the release of proinflammatory cytokines. IL-17A further induces the production of chemokines, leading to the recruitment of neutrophils that contribute to local inflammation. IL-22 does not act on immune cells but various tissue cells. IL-22 induces the release of antimicrobial peptides and other factors and can contribute in this way to bacterial elimination. At least in the infection with T 17 cells can be protective. However, the combined release of TNF and IL-17A is non-beneficial and exerts pathological effects. Inhibition of either TNF or IL-17A in this situation renders T 17 cells as protective as T 1 cells ( )",yes
PMC3693953,Figure_24,oa_package/ad/7f/PMC3693953.tar.gz,[],"Figure 24 (Top) Activation of glial cells in the retina. Astrocytes connecting vessels with neurons (left) and irregular pattern of activated astrocytes (right). (Middle and bottom) Activated astrocytes increase light scattering, visible in red-free light (right), but not visible in colour photos (left). (From [ ] (top), [ ] (middle) and [ ] (bottom), with permission).",yes
PMC2270292,Figure_3,oa_package/56/95/PMC2270292.tar.gz,"['\nMuc2 Mutant Mice Develop Spontaneous ColitisThe progressive incidence of rectal prolapse and colitis-associated mortality in Winnie and Eeyore mice is shown in A.', 'Proximal and distal colon were thickened and colon weight was greater in Winnie and Eeyore than in wild-type mice, and the thickening and weight increased progressively from 6 to 18 wk of age (B).', 'Colitis was assessed histologically in Winnie mice at 6, 12, and 18 wk of age, revealing mild inflammation in the large intestine (C).', 'Classical signs of murine colitis, including crypt elongation, neutrophilic infiltrates, goblet cell loss, crypt abscesses, and focal epithelial erosions were present, particularly in the distal large intestine (examples shown in F 3K).', 'Spontaneous Colitis in Mice with Muc2 Mutations(A) Incidence of premature death or colitis-associated pathology requiring humane killing (chiefly rectal prolapse) in Winnie (n = 309) and Eeyore (n = 355) mice.']","Figure 3 Spontaneous Colitis in Mice with Muc2 Mutations (A) Incidence of premature death or colitis-associated pathology requiring humane killing (chiefly rectal prolapse) in ( = 309) and ( = 355) mice. Mice entering experiments or killed for other reasons were treated as censored observations (designated by upward ticks), and the number of uncensored mice remaining at 50 d intervals is included underneath the graph. Incidence rates in the two strains were compared using the Mantel Log-rank test. (B) Weight of the colon after removal of luminal faecal material in C57BL/6 (WT), ( ), and ( ) mice at 6 ( 69 wk), 12, and 18 (WT and only) wk of age, = 49; box plots show median, quartiles, and range. (C) Histological colitis scores (see ) in WT and mice at 6, 12, and 18 wk of age, = 46; scores from individual mice are shown. (DK) Histology of normal distal colon from a C57BL/6 mouse (D and E) and examples of inflammation in the rectum (FI) and distal large intestine (J and K) of untreated mice showing leukocytic infiltration (G and I), occasional branching crypts (F and H), crypt abscesses (J and K) and focal ulcerations (I); scale bars = 20 m. Note the layer covering the mucosal surface in (F) is a granulocytic serous exudate. Statistics (B and C): -values for Kruskal-Wallis nonparametric analysis are shown, Dunn's multiple comparison test versus wild type, ** < 0.01, *** < 0.001; versus at 6 wk, < 0.05; versus at 6 wk, < 0.05.",yes
PMC6636106,Figure_4,oa_package/4a/17/PMC6636106.tar.gz,"[' 4).', '4) were present in 20/31 MCNs, while 2 septa were found in 11/31 cases.', 'Mucinous cystic neoplasm with round shape and unilocular cyst']",Fig. 4 Mucinous cystic neoplasm with round shape and unilocular cyst,yes
PMC6704256,Figure_7,oa_package/be/c8/PMC6704256.tar.gz,"[' 7a).', ' 7c, d zoomed image of CAA in D).', ' 7a) a formulation that provides robust peripheral CSF1R inhibition without microglial elimination17.', ' 7b), and accordingly, microglia numbers were unchanged relative to untreated animals (', ' 7e i).', ' 7f, h, j k).', 'Administration of an analogous CSF1R inhibitor, PLX3397 (75 ppm and 600 ppm), to 5xFAD mice.', 'Error bars indicate SEMGene expression analyses across three brain regionsGiven the prolonged absence of microglia throughout the adult life of CSF1R-inhibitor-treated wild-type and 5xFAD mice, the stark reduction in plaque formation in the absence of microglia, and the differences in pathology deposition and microglial number in various brain regions (i.']","Fig. 7 Administration of an analogous CSF1R inhibitor, PLX3397 (75 ppm and 600 ppm), to 5xFAD mice. Experimental design. Terminal PK of wild-type and 5xFAD groups treated with PLX3397. , Confocal images of tissue stained for dense-core plaques (Thio-S in green) and immunolabeled for microglia (IBA1 in red) in 600 ppm PLX3397-treated and control mice. Scale bar=75m. Sections of the retrosplenial (RS) and somatosensory (SS) cortex, respectively, stained for dense-core plaques (Thio-S in green) and immunolabeled for microglia (IBA1 in red) in mice treated with control or 75 ppm PLX3397. Scale bar=75m. Quantification of IBA1 cell number in the RS and SS cortex. (SS Cortex: PLX5622 v 5xFAD+PLX5622, =0.045) Two-way ANOVA with Tukeys post hoc test; =4 for Wild-type, =6 for PLX3397, =6 for 5xFAD, =4 for 5xFAD+PLX3397. , Quantification of cortical plaque number and volume, respectively, revealing no change in these measures with 75 ppm PLX3397 treatment in 5xFAD mice. Two-tailed independent -test; =45 for 5xFAD, =45 for 5xFAD+PLX3397. Statistical significance is denoted by * <0.05. Statistical trends are denoted by <0.10. Error bars indicate SEM",yes
PMC11506919,Figure_3,oa_package/61/88/PMC11506919.tar.gz,"['9% in the spleen (A).', '062) compared to Lal KO mice (B).', 'No alterations in the Lin- Sca-1+ c-Kit+ fraction was observed in any of the genotypes (C).', 'The only significant change was an increase in the Lin- c-Kit+ compartment of Lal KO mice (C).', 'The common myeloid progenitors as well as the megakaryocyte erythrocyte progenitor fraction was comparable in all genotypes (D).', 'In agreement with previous data [3], the granulocyte macrophage progenitor fraction was significantly increased in Lal KO mice, and comparably elevated levels were found in Lal/Mmp12 DKO mice (D).', 'Reduced CD11b+ Ly6G+ counts in blood, bone marrow, and spleen, but no changes in the hematopoietic progenitor compartment of Lal/Mmp12 DKO mice.']","Figure 3 Reduced CD11b+ Ly6G+ counts in blood, bone marrow, and spleen, but no changes in the hematopoietic progenitor compartment of DKO mice. ( ) Representative flow cytometric plots and their quantification, depicting a decreased CD11b+ Ly6G+ fraction in peripheral blood, bone marrow, and spleen of DKO compared to KO mice. ( ) Spleen weight normalized to brain weight; WT mice were arbitrarily set to 1. ( ) The content of Lin- Sca1+ c-Kit+ (LSK) and Lin- c-Kit+ (LK) and ( ) progenitor cells [common myeloid progenitors (CMPs), granulocytemacrophage progenitors (GMPs), megakaryocyteerythrocyte progenitor cells (MEPs)] shown as % of 7-AAD lineage-negative bone marrow cells. Data are shown as means (n = 410) + SD. ** < 0.01 and *** 0.001 for the comparison between WT and Lal KO mice; 0.05 and 0.01 for the comparison between KO and DKO mice.",yes
PMC10407717,Figure_4,oa_package/61/76/PMC10407717.tar.gz,"['In May 2022, the whole abdominal enhanced CT scan was performed and it was found that the mass in the abdominal and pelvic cavities had decreased ().', '.']","Figure 4. Abdominal enhanced computed tomography scan illustrating multiple masses, such as fat and soft tissue foci, in abdominal and pelvic cavities. The head of the pancreas was partly involved (May 27, 2022). (A) Abdominal mass (10.296.24 cm). (B) Pelvic mass (17.1513.00 cm).",yes
PMC10619492,Figure_1,oa_package/58/3f/PMC10619492.tar.gz,"['We found that Bmal1, Clock, and Per2 were downregulated in aged (older than 12 months) skin compared with young (approximately 3 6 months) skin (A).', 'Serial passaging of primary human keratinocytes, which acts as a surrogate for human skin aging (15), showed that circadian transcriptional activity decreased with increasing passage numbers (B).', 'Using quantitative real-time PCR (qRT-PCR) and immunofluorescence, we found that mRNA and protein levels of AVPs (Oas1a, Oas2, Ifitm1) were significantly reduced in aged murine skin compared with young skin (, C and D) and visualized in epidermal structures and sebaceous glands (17).', 'We observed that wounding significantly elevated AVP induction at 24 hours after wounding in young and old skin; however, the magnitude of induction was significantly higher in wounds of young skin compared with that of aged skin (E).', 'To address if aging affects CD301b+ signaling, we examined murine skin across ages for the presence of CD301b+ cells via immunostaining and found that CD301b+ cells were reduced in the skin of aged compared with younger mice (F).', 'Flow cytometry analysis revealed that wounded aged skin had a decreased influx of CD301b+ cells and expression of IL-27 (, G and H) (the gating strategy is shown in Supplemental ; supplemental material available online with this article; 11752295Version 109/19/2023In-Press PreviewVersion 210/23/2023Electronic publicationAging skin exhibits diminished circadian, AVP, and IL-27 transcription.', 'The flow cytometry gating strategy is included in Supplemental .']","Figure 1 Aging skin exhibits diminished circadian, AVP, and IL-27 transcription. ( ) Quantitative PCR (qPCR) of , , and in aged ( = 1416, >1 year old) and young ( = 3234 1 month old) male murine skin. Graphs represent averages of relative mRNA SEM with GAPDH used for an internal control. values were obtained via 2-tailed Students test. ( ) qPCR of , , and in human primary keratinocytes over serial passaging ( = 23 donors/passage). Graphs represent averages of relative mRNA SEM with GAPDH used for an internal control. ( ) qRT-PCR of , , and in aged and young murine back skins as described in . values were obtained via 2-tailed Students test. ( ) Immunostaining for OAS1a (orange) and nuclei (blue) in aged and young nonwounded skin. Scale bar: 25 m. ( ) qRT-PCR of AVP in young and old skin 24 hours after wounding. Graphs represent averages of relative mRNA SEM with GAPDH used for an internal control. values were obtained via 2-way ANOVA with multiple comparison. NW, nonwounded skin; W, wounded skin. ( ) Immunostaining for CD301b (green) and nuclei (blue) in aged and young unwounded skin. Scale bar: 25 m. ( ) Flow cytometry showing reduced numbers of CD301b cells in aged skin compared with young skin ( = 4 mice/group) as a percentage of total harvested live, single cells. values were obtained via 2-way ANOVA with multiple comparisons. ( ) Histogram displays IL-27 production from CD11b (yellow) and CD301b (blue) cells compared with CD45 (red). The flow cytometry gating strategy is included in . * 0.05, ** 0.01, *** 0.001, 0.0001.",yes
PMC10218270,Figure_2,oa_package/f6/a4/PMC10218270.tar.gz,"['Clinical images of patient 5, (a) before starting treatment with vismodegib, (b,c) during follow-up visits, and (d) after completion of treatment.']","Figure 2 Clinical images of patient 5, ( ) before starting treatment with vismodegib, ( , ) during follow-up visits, and ( ) after completion of treatment.",yes
PMC10419562,Figure_1,oa_package/fb/dc/PMC10419562.tar.gz,['9).'],Figure 1. Classification of galactography.,yes
PMC11353024,Figure_10,oa_package/1b/19/PMC11353024.tar.gz,[],"Figure 9 Effect of AVR-123 treatment on mouse splenic immune cells from the OIR mice. ( ) The splenic immune cell populations from OIR mice injected IP with AVR-123 (P7P12) were collected at P18, and the cells ( = 3 mouse spleens) were stained with immune cell-specific antibodies and analyzed via flow cytometry. ( , ) The splenic immune cell populations from OIR mice treated with AVR-123 (ED) during P12P17. Treatment with AVR-123 reduced populations of macrophages (F4/80+CF11b+), CD8 T cells (CD3+CD8+), neutrophils (Ly6G+CD11b+), and dendritic cells (CD11c+MHCII+) in mouse pup spleens compared to the hyperoxia mouse group. = 3. * < 0.05, -test.",yes
PMC8018011,Figure_2,oa_package/ef/d1/PMC8018011.tar.gz,['\nCharacterisation of huMG using mass cytometry.'],"Figure 2 . Donor (for brain autopsies) and/or patient (for biopsy tissues) cohorts including the control group are selected depending on accessibility, ethics and the experimental questions to be investigated. HuMG and other immune cells (optional) of interest can be isolated from different brain regions/compartments. At least 10 cells per aliquot is recommended. A cell isolation strategy is chosen based on tissue types and cell type abundance and/or vulnerability (e.g. to enzymatic digestion). To minimise the contamination of isolated huMG fraction by remaining myelin and cell debris, an additional step of cell enrichment such as the fluorescence or magneticactivated cell sorting can be included. Isolated cells can be either analysed directly or cryopreserved as either live or fixed cells. In the case of immediate analysis, a workflow/protocol for sample normalisation is required. Live/dead fixable staining can be performed prior to cell fixation (optional) and cryopreservation. To reduce interrun and technical variation, samples can be barcoded, pooled, stained and acquired as a single sample. It is advisable to include anchor samples to facilitate the data normalisation, and negative and positive control cells to enable the validation of antibody affinity and specificity.",yes
PMC6105147,Figure_2,oa_package/0e/24/PMC6105147.tar.gz,"[' 2) or markedly increased tracer uptake on 18F-FDG PET/CT (', 'Note the lack of extension posterior to the epidural spaceA 75-year-old man (patient 2).']","Fig. 2 A 70-year-old man (patient 3). Sagittal T2, T1 pre-contrast and T1 post-contrast sequences demonstrating increased T2-weighted signal centred on the L4/5 disc with anterior paravertebral extension corresponding to a peripherally enhancing abscess. Axial T1 pre-contrast and T1 post-intravenous contrast medium at the L4/5 disc level show contiguous peripherally enhancing disc/anterior paravertebral abscess. Note the lack of extension posterior to the epidural space",yes
PMC10139823,Figure_4,oa_package/13/02/PMC10139823.tar.gz,['CT image of a healthy lungCT image of healthy lungs showing normal CT ratio.'],Figure 4 CT image of a healthy lung CT image of healthy lungs showing normal CT ratio. Normal bronchovascular markings can be visualized. The image is adapted from (free access). Image source: Reference no. [ ],yes
PMC11566127,Figure_2,oa_package/48/d1/PMC11566127.tar.gz,['MRI/US-guided prostate biopsy.'],"Figure 2 MRI/US-guided prostate biopsy. (A) MRI/US-guided biopsy of the prostate involves initially performing MRI scans of the prostate and rendering a 3D model of the prostate (blue spherical structure), including the location of the suspected tumour using these data. (B) Diffusion-weighted and ADC images can then further elucidate the position of the tumour (coloured green and red). (C) Subsequently, this 3D model, including the tumours position, is fused in real time with US imaging, allowing the operator to precisely sample the tumour. The orange line demarcates the outline of the prostate, and the suspected tumour outlined by a white line. Fusing the images allows the operator to accurately pinpoint the needle projection, as shown by the green and orange circles. ADC: apparent diffusion coefficient; MRI: magnetic resonance imaging; US: ultrasound.",yes
PMC7338986,Figure_1,oa_package/43/d1/PMC7338986.tar.gz,"['Noncontrast axial head CT, bone window demonstrates groundglass mineralization of the central skull base (arrows) with subtle cortical irregularity (arrowhead) suggesting an underlying inflammatory or proliferative process.', ' Noncontrast coronal head CT, in a bone window, demonstrates a left middle ear effusion (black arrows).']","Fig. 1 Noncontrast axial head CT, bone window demonstrates groundglass mineralization of the central skull base (arrows) with subtle cortical irregularity (arrowhead) suggesting an underlying inflammatory or proliferative process.",yes
PMC10395478,Figure_5,oa_package/0b/35/PMC10395478.tar.gz,"['The above results indicate that the intervention of RGFP966 activates the HDAC3/Nrf2 signaling pathway and increases the expression of antioxidant factors ().', '3DiscussionOur research proved that the high selective HDAC3 inhibitor RGFP966 [25]had an inhibitory effect on HDAC3 in experimental rat SBI models, it could significantly reduce nerve function injury, brain edema, nerve cell apoptosis and neurodegenerative death induced by SBI in rats.', 'By reducing the action of HDAC3, RGFP966 increased the expression of Nrf2, improved nerve function, reduced brain edema, apoptosis and oxidative stress, thereby reduced the brain damage of SBI ().']","Fig. 5 Mechanism diagram RGFP966 plays a neuroprotective role in surgical brain injury through the HDAC3/Nrf2 pathway. SBI induces a rapid increase of endogenous HDAC3 expression and blocks the Nrf2 pathway. RGFP966 reversed the trend of increased oxidative stress and apoptosis of nerve cells after SBI, and alleviated nerve injury. Arrows indicate activation; Bars represent suppression.",yes
PMC6055756,Figure_4,oa_package/55/58/PMC6055756.tar.gz,"['Mass spectrometric imaging of the amyloid deposits.', '7 from the mouse intestine tissue section presented in \n S2.', '7 from the mouse intestine tissue section presented in .', 'Same section stained with Congo red after MALDI IMS (left); this Congo red panel is the same as shown in .']","Figure 4 Mass spectrometric imaging of the amyloid deposits. (A) Correlation between Congo red and MALDI images of a mouse intestine tissue sample. MALDI IMS distribution of specific ion peptides identified as Apoe with values 1075.6, 1239.7, 1510.9, 1599.9, and 1743.9; Apoa4 with values 1131.7, 1231.7, 1424.8, and 1461.8; and Apoa1 with value 1299.7 correlates with Congo red positive staining. (B) MALDI IMS distribution of the same ion peptides detected on a different mouse intestine tissue sample. A predictive peptide ( 764.4) for Apoe protein was also detected correlating exclusively with the amyloid deposits. Overlay of Congo red and MALDI images shows colocalization of the different species with extensive overlap with the amyloid deposits. MALDI IMS analysis was performed on the adjacent section to the Congo red staining section. The chromatic scale color bar indicates the relationship between the color of a peak displayed and the peak intensity in arbitrary units, with red and blue indicating the highest and lowest intensity color, respectively.",yes
PMC7736862,Figure_3,oa_package/ab/f4/PMC7736862.tar.gz,"[' 3a).', ' 3b).', ' 3c), while N-cadherin protein expression majorly increased upon treatments with all holo-Tf concentrations (p 0.', ' 3d).', 'Effect of holo-transferrin on metastatic marker N-cadherin.', 'Iron induced the expression of mesenchymal marker vimentinLike N-cadherin mRNA (CDH2) expression, vimentin mRNA expression increased upon 1 g/L and 2 g/L treatments after 24 h (', ' 3a,d), suggesting a time-delay between their mRNA and protein expressions.', ' 3) (as hypothesised) and E-cadherin responses (', ' 3b).', ' 3c,d) and vimentin (']","Figure 3 Effect of holo-transferrin on metastatic marker N-cadherin. HepG2 cells were treated with holo-Tf for 24h ( , ) and 48h ( , ). Gene expression of N-cadherin ( , ) and levels of N-cadherin protein ( , ) were determined by real-time PCR and western blot, respectively. Images are cropped versions. Full length images are in Supplementary Fig. . Protein intensity was analysed by the Image-J software available at the National Institute of Health (USA), downloadable from as of August 2020. Data is presented as meanSEM (n=34). * <=0.05 and ** <=0.03 compared to untreated control (0g/L).",yes
PMC9682730,Figure_4,oa_package/af/a4/PMC9682730.tar.gz,"[' 4), suggesting that partial loss of spastin function contributes to the toxicity of CHMP2BIntron5 in vivo.', '0001 by chi-square analysisLoss-of-function mutations in SPAST cause spastic paraplegia 4 (SPG4) [20, 21], the most common autosomal dominant form of HSP, which can be associated with clinical dementia [25].']","Fig. 4 Functional significance of the interaction between spastin and CHMP2B in a model of FTD3. Representative images of fly eyes with different genotypes. Quantification of the retinal degeneration phenotypes in expressing flies with or without knockdown. The number of flies of each genotype is shown under the -axis. The percentages of flies with severe, medium, or weak eye phenotypes are shown in the columns. **** <0.0001 by chi-square analysis",yes
PMC11278456,Figure_2,oa_package/6e/d3/PMC11278456.tar.gz,"['By integrating clinical manifestations, laboratory test results, and imaging findings, clinicians can more accurately diagnose and manage UE, reducing the risk of misdiagnosis and ensuring better patient outcomes ().', 'FLAIR images on the axial plane show the anatomical structures involved in UE and their main clinical presentations.']",Figure 2 FLAIR images on the axial plane show the anatomical structures involved in UE and their main clinical presentations.,yes
PMC5775400,Figure_8,oa_package/2e/93/PMC5775400.tar.gz,"['46 Intersection of the P21 DEGs with 75 DEGs previously reported in mdm mice46 revealed a significant overlap of 26 genes (a).', '28 Periodic acid Schiff (PAS) staining revealed abnormal glycogen deposits in TA and soleus fibers of Spin1M5 mice (b, black triangles).', 'Therefore, we analyzed neuromuscular junctions in the diaphragm of newborn and adult Spin1M5 mice by electron microscopy (c).', 'At both time points, the analysis revealed defects of the synaptic membrane (white triangles) and an abnormal appearance as well as a reduced number of synaptic vesicles (arrows) in Spin1M5 mice (c).', 'Furthermore, we observed the presence of vacuoles at nerve terminals of adult Spin1M5 mice (c, right columns (asterisks)).', 'org/1999/xlink"" xlink:href=""cddis2017468f7""/>Differential gene expression in surviving Spin1M5 mice correlates with SkM disease patterns.']","Figure 8 Differential gene expression in surviving Spin1 mice correlates with SkM disease patterns. ( ) Intersection of DEGs observed for the TA of Spin1 mice at P21 (blue) and SkM of mice (gray) representing a model for tibial muscular dystrophy (TMD) (R: enrichment factor; p: -value calculated for the intersection). ( ) Periodic acidSchiff (PAS) staining for glycogen accumulation in TA and soleus muscle (SOL) of Spin1 and control mice at 30 weeks of age. Fibers showing abnormal glycogen accumulation are marked with black triangles. ( ) Electron microscopy (EM) images of neuromuscular junctions in the diaphragm (DP) of newborn and 15-week-old Spin1 and control mice. Membranes at the synaptic cleft are marked with white triangles, synaptic vesicles with arrows, and vacuoles observed at neuromuscular junctions of Spin1 mice with asterisks",yes
PMC8493969,Figure_5,oa_package/5c/4f/PMC8493969.tar.gz,[],"FIGURE 5 Representative traces of shortcircuit current in CFTR F508del and G542X sheep tracheal epithelial cell cultures. Primary cultures of sheep tracheal epithelial cells were seeded onto permeable filter supports and maintained at the ALI for 6weeks. At the time of measurement, the filter inserts were placed in Ussing chambers and bathed on both sides with KrebsRinger bicarbonate solution bubbled with 95% O /5% CO and maintained at 37. (A) Effect of CFTR correctors on F508del cells. Cultures were pretreated with VX809, VX661, or VX445 at 3.3M for 24h. At the indicated times, amiloride (Amil, 100M, apical), forskolin (For, 10M, basolateral), VX770 (1M, apical & basolateral), genistein (Gen, 30M, apical & basolateral), and GlyH101 (20M, apical) were added for acute treatment. (B) Effect of G418 and SMG1i on G542 cells. Cells were pretreated with SMG1i (1M) plus G418 (50M) for 24h with or without VX661 (3.3M), followed by acute treatment as described above",yes
PMC11333867,Figure_12,oa_package/e3/99/PMC11333867.tar.gz,[],Figure 12 A medical image from Pathology Education Informational Resource-Gross data set.,yes
PMC7352008,Figure_1,oa_package/6e/1f/PMC7352008.tar.gz,"['MAs were defined as hyperfluorescent dots on the FA images () and visible aneurysms on the OCTA images ().', 'MAs in eye with BRVO.']","Figure 1 MAs in eye with BRVO. ( ) Early-phase FA. ( ) OCTA. The MAs (yellow circles in panel ) appear as dot-shaped hyperfluorescent spots on the FA image. It is difficult to classify the MAs morphologically using FA. In contrast, OCTA enables morphologic analysis of MAs (yellow circles in panel ).",yes
PMC11353024,Figure_4,oa_package/1b/19/PMC11353024.tar.gz,"['AVR-123 Inhibits VEGF-Induced Vascular Tube and Microcapillary FormationHuman retinal endothelial cells (HRECs) were used to demonstrate the effect of AVR-123 on neovascularization in an in situ model ().', 'As shown in A, we anticipated that inhibiting TLR2 signaling would reduce angiogenesis and new tube formation.', 'Evaluation of anti-angiogenic activity in human retinal endothelial cells (HRECs) following Matrigel tube formation assay.']","Figure 3 Receptor inhibition and cytokine assays. Briefly, compounds AVR-121 and AVR-123 (1, 10, and 100 M) were dosed with PMA (200 ng/mL)-activated THP-1 cells and were incubated for 48 h. ( , ) TLR2 and TLR4 protein levels in cell lysate were probed via ELISA. ( , , ) Activated THP-1 cells were treated with either LPS or PAM CSK or in combination with AVR-121 and AVR-123 for 48 h, and cytokines TNF- and IL-1 were assessed in the supernatant via ELISA. ( ) CBMCs were treated with AVR-123 (100 M), LPS, and TAK-242 (TAK) for 24 h and assessed for cytokines IL-6, IL-1, and TNF- in the cell supernatant via ELISA. N = 3, data are presented as mean SEM, * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001. One-way ANOVA, Graph Pad Prism.",yes
PMC10730449,Figure_4,oa_package/b5/cb/PMC10730449.tar.gz,"['20 Imaging features are not characteristic, appearing echogenic with significant vascularity on ultrasound and as homogeneous masses with intense enhancement on CT ().']","FIGURE 4 A 49-year-old male with non-specific abdominal pain was found to have a filling defect (arrowhead) in the inferior vena cava on the contrast-enhanced coronal CT image (a) and axial (b), which was histopathologically proven to be papillary endothelial hyperplasia.",yes
PMC7528343,Figure_5,oa_package/19/e8/PMC7528343.tar.gz,"['5a).', '5b], indicating that the Dnmt3bMommeD14 variant does not have a major impact on histone modifications of the D4Z4 repeat array.', '5c].', 'DNA methylation at the D4Z4 repeat array, but not the chromatin compaction score, is affected by the Dnmt3bMommeD14 variant.', 'The error bars represent the SEM for two biological replicatesSpecific immune cell populations in the secondary lymphoid organs of D4Z4-2.']","Fig. 5 DNA methylation at the D4Z4 repeat array, but not the chromatin compaction score, is affected by the Dnmt3b variant. The average DNA methylation level of 10 CpG dinucleotides just distal to the D4Z4 repeat (the FasPas region) was not changed in tail DNA of D4Z4-2.5/Dnmt3b mice (postnatal day 15). In contrast, in spleen DNA a significant reduction in DNA methylation levels was detected in D4Z4-2.5/Dnmt3b mice compared to D4Z4-2.5 mice ( = 0.026). Each dot represents the average DNA methylation level of 10 CpG sites per mouse. Differences between groups were compared using a Students test. * < 0.05. n.s. = not significant. 2.5 = D4Z4-2.5; MD14 = Dnmt3b . The chromatin compaction score (H3K9me3 level corrected for H3K4me2 level) was not different in the spleen of D4Z4-2.5/Dnmt3b mice compared to D4Z4-2.5 mice (postnatal day 15) as measured by ChIP-qPCR analysis. Each dot represents one mouse. Statistical analysis was performed using a Students test. n.s. = not significant. 2.5 = D4Z4-2.5; MD14 = Dnmt3b . Dnmt3b is not enriched at the D4Z4 repeat in the spleen of D4Z4-2.5 mice (postnatal day 15) as determined by ChIP-qPCR. The error bars represent the SEM for two biological replicates",yes
PMC8041673,Figure_6,oa_package/49/b4/PMC8041673.tar.gz,"['In case of graft instability, the option of using one or two screws to stabilize the graft always remainsClosure of the distal tibial osteotomy and incision layers.']","Fig. 6 Once the optimal fitting shape has been reached, the autograft is transported and placed into the host site( ). By means of an impactor the inserted autograft can be fitted exactly 12mm underneath the level of the talar cartilage. The curvature of the iliac crest accurately matches the talar curvature( ). As this is apress-fit technique, no additional screws are necessary in order to fixate the autograft. In case of graft instability, the option of using one or two screws to stabilize the graft always remains",yes
PMC11233046,Figure_3,oa_package/c9/cb/PMC11233046.tar.gz,['Giemsa stain was positive for Donovan bodies [].'],Figure 3 Giemsa stain was positive for Donovan bodies (100),yes
PMC10419317,Figure_12,oa_package/24/d9/PMC10419317.tar.gz,[],"Figure 12 ( ) Delimiting region between a parathyroid adenoma (adm) and the peripheral compressed parathyroid gland (prt). ( , ) TCs/CD34+ SCs (brown, arrows) are seen around vessels (v) and parenchymal nests in areas of the adenoma immediately adjacent to the delimiting region. Note the absence of TCs/CD34+ SCs around small vessels (arrowheads) away from this region ( ). ( ) Little or no presence of collagen I is observed where TCs/CD34+ SCs are absent. ( , ) Double immunochemistry for CD34 (brown) and SMA (red). ( ) Immunochemistry for CD34 (brown). ( ) Hematoxylin counterstain. ( ) Double immunofluorescence for CD34 (green) and collagen I (red). DAPI counterstain. Scale bars: ( ) 120 m, ( ) 15 m, ( ) 50 m, ( ) 20 m.",yes
PMC10159294,Figure_4,oa_package/b6/84/PMC10159294.tar.gz,"['8%) [].', ':A 63-year-old woman with coronavirus disease 19 pneumonia who presented with severe dyspnea and fever.']","Figure 4: A 63-year-old woman with coronavirus disease 19 pneumonia who presented with severe dyspnea and fever. High resolution computed tomography (CT) image on coronal plane shows bilateral parenchymal consolidation occupying >75% of lung parenchyma, with CT total severity score Grade 4.",yes
PMC10795236,Figure_5,oa_package/47/ef/PMC10795236.tar.gz,"['\nPathological examination revealed a papillary polypoid\nlesion with fibrotic and vascularized stroma and\nciliated columnar and squamous metaplastic\nepithelium and was reported as mixed bronchial\npapilloma\n(\n\n,\n\n).', '\nPresence of papillary projections in the polyp.']",Figure 5 Ciliated and squamous epithelial transition on thepolyp surface.,yes
PMC10591407,Figure_3,oa_package/94/a2/PMC10591407.tar.gz,"['db/db group\nAct protected podocytes and inhibited podocytes apoptosis in db/db mice and MPC5 cellsWe then determined whether Act exerted a protective effect on podocytes in db/db mice and on MPC5 cells ( 4).', ' 3d-f).', '\nAct protected renal podocyte in db/db mice.', 'docx"">.']","Fig. 3 Act protected renal podocyte in db/db mice. ( ) Immunohistochemistry of synaptopodin and podocin in mice (Scale bar =50m); ( ) The western blot consequences of synaptopodin and podocin. The results were shown as meansSD. #P<0.05, ##P<0.01, and ###P<0.001 vs. db/m group; *P<0.05, **P<0.01, and ***P<0.001 vs. db/db group",yes
PMC9670392,Figure_2,oa_package/4d/c7/PMC9670392.tar.gz,"[' 2).', 'Fixed deformation during surgery.', 'Note the wedge removalPlantigrade foot after wedge resectionPostoperatively, patients were hospitalized for a few days to a week to avoid long-distance travel immediately after the procedure and evaluate short-term complications.']",Fig. 2 Fixed deformation during surgery. Note the wedge removal,yes
PMC9483895,Figure_10,oa_package/aa/f9/PMC9483895.tar.gz,[],"Fig. 10 Assessments of potential phenotypic impacts of LTR5_Hs elements based on GSEA of 486 high-fidelity down-steam target genes employing the Transcription Factors (TF) perturbations followed by expression database ( ; , left panel), the Cell Marker Augmented 2021 database ( ), the Human RNA seq Automatic GEO Signatures database ( ; , right panel). In panels results illustrating the top 10 significantly enriched records are reported. In (D), top 30 significantly enriched records of gene sets are reported. All reported significantly enriched records were identified at the significance threshold of adjusted value<0.05 by the GSEA of 486 LTR5_Hs-regulated genes employing the corresponding genomic databases (Methods)",yes
PMC8790165,Figure_8,oa_package/a5/41/PMC8790165.tar.gz,"['32 Type I is related to lymphoproliferative disorders of B lymphocytes, such as multiple myeloma, and manifests as a thrombotic vasculopathy ().', 'Cryoglobulinemic thrombotic vasculopathy.', 'Hypocomplementemic urticarial vasculitis presents with urticarial plaques that last for more than 24 hours, are often more painful than pruriginous, and leave local pigmentation.']",Figure 8 Cryoglobulinemic thrombotic vasculopathy. Type 1 cryoglobulinemia coursing with thrombotic vasculopathy: erythematous and purpuric macules with jagged outline and central necrosis.,yes
PMC6259024,Figure_2,oa_package/fc/31/PMC6259024.tar.gz,['Intraoperative views.'],Figure 2 Intraoperative views. The image of the anastomosis formed with circular stapler between the third part of the duodenum and proximal jejunum after the resection. Circumferential serosal sutures with prolyene were placed to reinforce the anastomosis.,yes
PMC11357072,Figure_2,oa_package/3e/0b/PMC11357072.tar.gz,"['During tissue collection from 16-month-old offspring, we observed that approximately 9% of offspring had developed macroscopic liver tumours (a), which was more than 3-times higher than reported rates of spontaneous tumour development across the lifespan of C57BL/6 mice [32].', 'Pathological characterization confirmed the majority of these tumours as HCC (a).', '89]) (a).', '01; b).', '04, c), while rates of HCC in female offspring were lower in HF/C and HF/HF mice compared to C/C, although this difference was not statistically significant (p = 0.', '10, d).', '(a) Gross morphology of a liver without any abnormalities (normal) and with large, macroscopic tumours (HCC).']","Figure 2 ( ) Gross morphology of a liver without any abnormalities (normal) and with large, macroscopic tumours (HCC). H & E staining showing normal cellular architecture in non-tumour tissue and enlarged trabeculae with steatosis in an HCC tumour. Quantification of the total number of male and female offspring with and without HCC. ( ), quantification of HCC by diet group ( ) and within male-offspring ( ) and female-offspring ( ) diet groups. Inset numbers represent the percentage of animals with HCC in each group. Scale bars = 20 m. * < 0.05, ** < 0.05, Fishers exact test.",yes
PMC4692518,Figure_3,oa_package/a5/59/PMC4692518.tar.gz,[],"Supplementary Fig.2 ROI quantification of FA, MD (10 m /s), ODI, NDI and IsoVF for each animal based on distinct anatomical regions (=p<0.05, =p< 0.01, =p<0.001).",yes
PMC4153268,Figure_3,oa_package/50/a5/PMC4153268.tar.gz,"['Cavitating lesion was not observed ( 6).', 'Hilar and carinal lymph node calcification (open arrow) and an extending mass to the left lung (filled arrow) in an anthracofibrosis patient.', 'Anthracotic mass lesions (, 4 and 6), which were proven to be free from pathological lesions, such as malignancy or tuberculosis (TB) by trans-thoracic needle biopsy, were observed in 14-17% of patients depending on the type of anthracosis (Tables 2 and 3).']",Figure 3 Hilar and carinal lymph node calcification (open arrow) and an extending mass to the left lung (filled arrow) in an anthracofibrosis patient.,yes
PMC5505051,Figure_7,oa_package/6f/9e/PMC5505051.tar.gz,['90 Days post-embolization.'],"Figure 7 90 Days post-embolization. (a) Unloaded control beads (yellow arrows) with interlobular fibrosis (blue arrows) and bile duct hyperplasia (green arrows) around reduced size lobules (x 1 magnification, scale bar = 2 mm). (b) VERB100 beads (yellow arrows) in the intermediate treated liver with mild bile duct hyperplasia (green arrows) (x 1 magnification, scale bar = 2 mm). (c) VERB100 beads (yellow arrows) integrated into the intermediate treated liver tissue with no signs of reaction or inflammation and mild interlobular fibrosis (blue arrows) (x 4 magnification, scale bar = 500 m). (d) Untreated portion of the liver of an VERB100 group animal for comparison, showing normal connective tissue separating lobules and normal liver tissue appearance (x 2 magnification, scale bar = 1 mm).",yes
PMC9514915,Figure_12,oa_package/1e/46/PMC9514915.tar.gz,[],"Fig. 12 A 70-year-old male patient with bronchogenic cyst. Axial chest computed tomographic (CT) images (top left and right) and coronal CT images (bottom left and right) show extension of bronchogenic cyst ( ) along the right lower mediastinal visceral space and anterior to the carina. The lower mediastinal visceral space narrows at this point ( ) and deforms the soft bronchogenic cyst, esophagus ( ).",yes
PMC4232697,Figure_6,oa_package/01/cb/PMC4232697.tar.gz,"['By quantitative analysis, we observed fewer amyloid plaques in both cortical and hippocampal regions of the brain in the 500 mkd Thiamet-G treated group ( 6).', 'Interestingly however, in the cortex we found that 200 mkd was sufficient to reduce the number of amyloid plaques to the same level as seen in mice treated with 500 mkd Thiamet-G despite this dose being unable to significantly reduce levels of A 42 as assessed by ELISA ( 6B).', '\nThiamet-G reduces plaque load in the TAPP mice.', 'Thiamet-G treatment does not influence release of A 42 from cellsIn an effort to address the question of how O-GlcNAc might affect -amyloid peptide formation we turned to using a well-established cell culture model used to evaluate molecular pathways influencing APP processing [35 38].']","Figure 6 . Representative 6E10 immunohistochemical analysis used to assess amyloid plaque load in both the cortical and hippocampal regions. . Quantitative assessment of 6E10 IHC analysis reveals that both 200 and 500 mkd Thiamet-G is sufficient to reduce the number of amyloid plaques in the cortex. . 500 mkd Thiamet-G significantly reduced the number of amyloid plaques in the hippocampus whereas 200 mkd Thiamet-G was ineffective. Error bars represent standard error of the mean ( S.E.M) and p-values result from students unpaired two-tailed t-tests. For all panels, N =12 in each group.",yes
PMC9463753,Figure_2,oa_package/5e/80/PMC9463753.tar.gz,['The patient underwent an MRI which was also suggestive of a malignant bone tumor ().'],"Figure 2 coronal T2 (A), STIR (B) and axial T1 weighted MRI without (C) and with contrast (D) showing metaphyseal-diaphyseal femoral process extending to the soft tissues and to the distal epyphysis",yes
PMC9395958,Figure_1,oa_package/45/8e/PMC9395958.tar.gz,"[' 1.', '0)DLE discoid lupus erythematosus, CHLE chilblain lupus erythematosus, LEP lupus erythematosus profundus, n number of lesionsClinical presentation of discoid lupus erythematosus (DLE) plaques on the a nose, b ear and c back.', 'f Videodermoscopy of the DLE plaques on the back: peripheral white scaling (black arrowhead) and white-yellowish scales (yellow arrowhead) in the center of the plaque, white pink structureless areas (black asterisks) and linear and linear curved (hairpin) vessels at the periphery (blue arrows)TrichoscopyTrichoscopic features of a total of 22 DLE lesions, including 18 lesions located on the scalp and 4 plaques within the eyebrows, were analyzed (Table 3).']","Fig. 1 Clinical presentation of discoid lupus erythematosus (DLE) plaques on the nose, ear and back. Corresponding videodermoscopic images. Videodermoscopic findings in the lesion on the nose: white scales ( ), follicular plugs ( ), white perifollicular halo ( ), pinkish structureless areas ( ) and peripheral gray-brown dots ( ). Videodermoscopic features of the DLE involving the ear: white scaling ( ) and multiple rosettes ( ). Videodermoscopy of the DLE plaques on the back: peripheral white scaling ( ) and white-yellowish scales ( ) in the center of the plaque, whitepink structureless areas ( ) and linear and linear curved (hairpin) vessels at the periphery ( )",yes
PMC5044858,Figure_2,oa_package/bd/d6/PMC5044858.tar.gz,"['Biochemical analysis of tissue homogenates from Tg(M83+/ :Gfap-luc+/ ) mice intraperitoneally injected with -synuclein fibrils showed that diseased mice had accumulated Sarkosyl-insoluble aggregates of phosphorylated -synuclein in their brains and spinal cords as determined by probing with the EP1536Y antibody, which recognizes phosphorylation at Ser129 of -synuclein (A).', 'In contrast, the brains and spinal cords of PBS-injected Tg(M83+/ :Gfap-luc+/ ) mice remained free of Sarkosyl-insoluble aggregates and showed staining only for the monomeric form of phosphorylated -synuclein (A).', 'Also, the mouse that died 285 days after intraglossal injection with -synuclein fibrils showed higher-molecular-weight bands characteristic of phosphorylated -synuclein pathology, but the mice that did not develop disease and the control mice that were injected with PBS did not (B).']","FIG 2 Biochemical analysis of phosphorylated -synuclein in the CNS of peripherally injected Tg(M83 : -luc ) mice. (A) Detection with the EP1536Y antibody, which recognizes phosphorylation at Ser129 of -synuclein, showed that diseased mice accumulated high-molecular-weight species of Sarkosyl-insoluble aggregates of phosphorylated -synuclein in their brains and spinal cords, whereas control mice challenged with PBS did not and showed bands only for the monomeric form of phosphorylated -synuclein. (B) After intraglossal challenge with -synuclein fibrils, only one mouse, animal 121, accumulated Sarkosyl-insoluble aggregates of phosphorylated -synuclein in the brain and spinal cord, whereas all other intraglossally inoculated animals remained healthy. Molecular masses are shown in kilodaltons. Sample loading in each lane is shown by detection of actin.",yes
PMC6659525,Figure_4,oa_package/bd/4f/PMC6659525.tar.gz,"['Where fluoroscopy is based on anatomical landmarks, USG provides real-time visualization of needle and CT confirms the location of needle tip in relation to surrounding structures [].', '[4142434445]Computed tomography-guided celiac plexus neurolysis: Needle is placed at the level of celiac axis (arrow) under computed tomography guidance.']",Figure 4 Computed tomography-guided celiac plexus neurolysis: Needle is placed at the level of celiac axis (arrow) under computed tomography guidance. Uniform spread of contrast-mixed neurolytic agent is seen (arrowheads),yes
PMC5964101,Figure_1,oa_package/1c/fc/PMC5964101.tar.gz,"[' 1a).', 'Pneumococcal secretome possesses DNase activity.', 'To test whether the DNase activity is conserved across pneumococcal serotypes, secretome from strains D39 (serotype 2; positive control), TIGR4 (serotype 4), ATCC 6301 (serotype 1), ATCC 6314 (serotype 14) and WU2 (serotype 3) was subjected to DNase assay (', ' 1b).', ' 1c e).', ' 1f).', ' 1g) revealed the presence of 39 proteins (Supplementary Table S2).', ' 1f) are subject of independent studies.', ' 1f).']","Figure 1 Pneumococcal secretome possesses DNase activity. ( ) Calf thymus DNA (CT DNA) was incubated in the presence or absence of secretome from deficient mutant of strain D39 grown in THY medium (D39 sec). The samples were incubated at 37C for 30min. The processed samples were resolved on an ethidium bromide stained agarose gel (0.8%; uncropped image). Bovine pancreatic DNase I (BP DNase) was used as a positive control. Secretome only and THY medium was taken as negative and vehicle control, respectively. ( ) DNase activity of the secretome (sec) derived from pneumococcal strains D39 (1; served as the positive control), TIGR4 (2), ATCC 6301 (3), ATCC 6314 (4) and WU2 (5). The DNase activity observed with the secretome obtained from D39 propagated in chemically defined medium (CDM) is heat labile ( ), and sensitive to treatment with proteinase K ( ) and EDTA ( ). Linear double-stranded plasmid DNA was used as the substrate in panels c-e. The molecular mass marker (in Kb) is shown. Heat, proteinase K and EDTA treatment is indicated by HT, PK and EDTA, respectively. ( ) Secretome from D39 was resolved on SDS-PAG bearing calf thymus DNA (30g/ml) followed by ethidium bromide staining as described in the . Three dark bands corresponding to DNA degradation were observed against fluorescent background (indicated using arrowheads). A portion of the gel has been enlarged to highlight the three bands. ( ) The bands corresponding to the three zones of DNA degradation were excised from a silver stained preparative polyacryamide gel, tryptic digested and subjected to mass spectrometric analysis. The molecular mass marker (in kDa) is shown on the right. The full-length gels for panels b-g can be viewed in Supplementary Fig. .",yes
PMC4182477,Figure_4,oa_package/a0/77/PMC4182477.tar.gz,"['As shown in A, the total levels of either Akt or ERK1/2 were similar between control and diabetic mice.', '01) (B) as compared to control, but disruption of GIGYF2 gene expression in hippocampus of diabetic mice resulted in a significant increase in the levels of phosphorylated ERK1/2 as compared to DM group (p 0.', 'g004The expression levels of Akt and ERK1/2.', 'Herein, we further examined the effects of downregulation of GIGYF2 expression in diabetic mice on the activation of AKT and ERK1/2 () and found that the decreased levels of phosphorylated Akt and ERK1/2 were correlated with the decreased level of phosphorylated IGF1R in diabetic mice, while down-regulation of GIGYF2 expression to normal endogenous levels in hippocampus of diabetic mice augmented ERK1/2 activation (', '4B), but did not modulate Akt activation (A).']",10.1371/journal.pone.0108559.g004,yes
PMC8820480,Figure_1,oa_package/6b/f3/PMC8820480.tar.gz,['Pelvic MRI images.'],"Figure 1 Pelvic MRI images. A: Axial T2-weighted image shows multicystic neoplastic masses (white arrows) with solid components in both ovaries. B: A peritoneal implant (blue arrow) with similar characteristics to the ovarian lesions is seen in the peritoneal cavity. C and D: T2-weighted images of the uterine cervix in the axial and sagittal plane reveal the bulky cervical tumor. The cervical stromal ring is well preserved (arrowhead). However, the anterior rectal wall continuum is not very well seen.",yes
PMC4518739,Figure_2,oa_package/f0/a9/PMC4518739.tar.gz,"['ResultsThe proposed method was evaluated on two separate image sets, that is, the STEI test set and the WSI test set (figure 2).']","Figure2 Flow chart of the main principle of the tumour viability assessment. The support vector machine (SVM) model is trained and optimised with the training set of single-tissue entity images (STEI), representing the different tissue categories of interest. The discrimination of the model is evaluated in parallel in test set of STEIs and in whole-slide images (WSIs). On the test STEI set, the agreement to classify a test image into viable or non-viable tumour category is evaluated by comparing result to manual labelling. Similarly on the WSI test set, the agreement in tissue segmentation and finally tumour viability assessment are evaluated by comparing obtained results with expert annotations.",yes
PMC11588180,Figure_4,oa_package/7a/bc/PMC11588180.tar.gz,"[':Examples of Medical Extended Realities Technology Used in Witowski et al.', 'Intraprocedurally, operators wearing HoloLens head-mounted displays were able to look at and interact with the 3D rendered digital images (developed from the pre-procedural imaging dataset) throughout the duration of the procedure using voice commands and hand gestures ().']",Figure 4: Examples of Medical Extended Realities Technology Used in Witowski et al.,yes
PMC4533565,Figure_2,oa_package/f7/f6/PMC4533565.tar.gz,"['(c) Hypomelanotic, confetti-like maculesHistopathologyHematoxylin-eosin (H and E)-stained sections prepared from the face, revealed a normal looking epidermis, small pilo-sebaceous units, and an evidence of increased dermal collagen [a].', 'At higher magnification, onion-peel - like concentric bands of collagen surrounding abortive hair follicles [b] were seen.', 'Concentric bands of fibrosis were also seen around some of the eccrine ducts [c], appearing to constrict their lumina.', '(a) Section, exhibiting a normal epidermis and small pilosebaceoous units (H and E, 40).']","Figure 2 (a) Section, exhibiting a normal epidermis and small pilosebaceoous units (H and E, 40). (b) Onion-peel-like concentric bands of collagen surrounding a hair follicle (H and E, 200). (c) An eccrine duct surrounded by concentric fibrosis (H and E, 400)",yes
PMC7485250,Figure_2,oa_package/9f/2e/PMC7485250.tar.gz,"['4Comparison of models trained with different FOVsWe trained six models using 10, 20 and 40 FoV tiles with sizes of 640 640 and 320 320 pixels, as illustrated in figure 2A.', 'In addition, with the help of larger tile size, the model trained with 10 FoV and 640 640-pixel tile size outperformed others on the validation set, shown in figure 2B.', '(A) An example of tiles in 10, 20 and 40 FoVs; (B) Tile-level classification accuracy on the validation set; (C) relative computing time of a WSI on different FoVs.', 'The inference time of different FoVs was given in figure 2C, all numbers were normalised by the time taken at 40.']","Figure 2 (A) An example of tiles in 10, 20 and 40 FoVs; (B) Tile-level classification accuracy on the validation set; (C) relative computing time of a WSI on different FoVs. FoVs, for different field of views.",yes
PMC4874602,Figure_10,oa_package/80/30/PMC4874602.tar.gz,[],"Figure 10 MALDI-MS time courseimages of BDQ and M2 in BALB/c mice acquired 8168 h followinga single oral 25 mg/kg dose of BDQ. Cellular, non-necrotizing lesions(type III) are outlined in white. Images depict a single lung lobeobtained from a representative mouse ( = 4 miceper time point). A serial section was stained with hematoxylin andeosin (H&E) for comparison.",yes
PMC3659080,Figure_1,oa_package/8d/3d/PMC3659080.tar.gz,['The levels of the presynaptic gutamatergic transporter VGlut in hippocampal neurons of 4-month-old apoE3 and apoE4 mice.'],Figure 1 VGlut1 immunohistochemistry. Representative images (X20 magnification) of the indicated hippocampal subfields are presented on the left. Quantification of the results (mean SEM: n=20 per group) of apoE3 mice (white bars) and apoE4 mice (black bars) was performed by computerized image analysis as described in Materials and Methods and is shown on the right. *p<0.001 (for comparison of the results of the apoE4 and apoE3 mice). Scale = 120 . VGlut immunoblot. Representative immunoblots of homogenates of whole hippocampi of apoE3 and apoE4 mice are presented on the left together with the GAPDH standard. Quantification of the results (mean SEM; n=10 per group) of apoE3 mice (white bars) and apoE4 mice (black bars) is depicted on the right. *p<0.001.,yes
PMC11292067,Figure_5,oa_package/ec/87/PMC11292067.tar.gz,"['The tumor was composed of very pleomorphic cells, and the cell nuclei were large, hyperchromatic with mitotic figure (staining method: HE, magnification: 4).']","Figure 5 The tumor was composed of very pleomorphic cells, and the cell nuclei were large, hyperchromatic with mitotic figure (staining method: HE, magnification: 4). HE, hematoxylin-eosin.",yes
PMC7734064,Figure_2,oa_package/91/18/PMC7734064.tar.gz,"['The surgical tumor tissues were subjected to target capture-based clinical next-generation sequencing (NGS) test targeting 31 cancer-relevant genes (Berry Oncology), and three independent driver mutations had been found in three nidi, respectively ().', '2240 2260delinsCAAGAG) (A) was detected at a mutant allele frequency (MAF) of 1.', '2311 2312delinsGGGTT) (A) was identified at a MAF of 5.', 'And EZR exon 10-ROS1 exon 34 (EZR-ROS1) rearrangement (B) was detected at DNA level in the other nodule (0.', 'NGS targeting gene test on the trilateral surgical tumor tissue.']","Figure 2 NGS targeting gene test on the trilateral surgical tumor tissue. ( ) Schematic diagram of 19del and 20ins. ( ) Schematic diagram of fusion. ( ) Integrative Genomics Viewer (IGV) snapshot of fusion regions, which was detected by NGS sequencing reads.",yes
PMC11312861,Figure_4,oa_package/0b/0c/PMC11312861.tar.gz,"['Clinically, within the left side of the body, epidermal (sebaceous) nevus syndrome on the trunk, and speckled lentiginous nevus on the head, neck, and chest were present (a,b).', 'We performed videodermoscopic examination with mapping of all types of secondary skin tumours (c), and, subsequently, 14 punch biopsy excisions were carried out of larger tumours with aim of ruling out syringocystadenocarcinoma.', 'Smaller diameter lesions, dermoscopically correlating with syringocystadenoma papilliferum, were treated with plasma device under local anaesthesia, with good aesthetic results, and NRS 6/10 (d).', 'The case of Phakomatosis pigmentokeratotica in lateralisation pattern.']","Figure 4 The case of Phakomatosis pigmentokeratotica in lateralisation pattern. ( ) Clinical presentation of the left side of bodythe segmental nevus spilus on the chest, and the segmental sebaceous nevus on the trunk; ( ) the surface of the sebaceous nevus was covered by numerous, ulcerated tumours, bleeding upon touchvascular malformations and syringocystadenoma papilliferum; ( ) the videodermoscopic examination revealed all components: nevus sebaceousagminated yellow clods surrounded by linear vessels ( ); syringocystadenoma papilliferumnodular lesion with pinkish and white background, and pearl-like structures at the rim of the centrally located ulceration ( ); and seborrheic keratoseskeratotic plugs on the cerebriform surface ( ) and keratotic and milia-like cysts within structureless brow area ( ); (Fotofinder Medicam HD 1000 Systems GmbH, Bad Birnbach, Germany); ( ) clinical presentation directly after the plasma device procedure.",yes
PMC10455608,Figure_3,oa_package/82/73/PMC10455608.tar.gz,"['Total body PET-FDG images showing pathologic uptake in septum and cardiac apex, also seen on cardiac PET-FDG in patient S.']","Figure 3 Total body PET-FDG images showing pathologic uptake in septum and cardiac apex, also seen on cardiac PET-FDG in patient S.Y. (case #10).",yes
PMC7543793,Figure_6,oa_package/a2/e5/PMC7543793.tar.gz,"['In \n, the learning curve and the loss curve obtained from the train and validation sets respectively show the stability of our model.', 'From we can observe that the training and validation losses decrease to a point of stability, and have a small gap between them.', '97Learning curve of our proposed model.', '\nExperiment 1: As shown in Table 2 for the segmentation and Table 3 for the classification, the best dice coefficient = 88%, accuracy (ACC = 0.']",Fig. 6 Learning curve of our proposed model. Left is the model loss and right is the model accuracy per epoch.,yes
PMC7571907,Figure_2,oa_package/ef/2a/PMC7571907.tar.gz,['Gastrointestinal endoscopy.'],Figure 2 Gastrointestinal endoscopy. Colonoscopy showing the dark-purple appearance of the mucosa of the ascending and transverse colon without ulcers.,yes
PMC5498906,Figure_5,oa_package/f1/ee/PMC5498906.tar.gz,"['Histologically, the lesion was consistent with a typical cholesterol granuloma; the granulation tissue contained clefts of cholesterol crystals, giant cells, and infiltration with inflammatory cells ().', '004""/>The pathological specimen showing a typical cholesterol granuloma.']","Figure 5 The pathological specimen showing a typical cholesterol granuloma. Granulation contains clefts of cholesterol crystals, giant cells, and infiltration of inflammation tissue (arrow). HE stain, 100x.",yes
PMC6079308,Figure_1,oa_package/25/df/PMC6079308.tar.gz,"['In the first cycle of ovarian stimulation, metformin and Gonal-f 75 IU for six days were prescribed () and then continued for two days.', 'Sonography before induction of ovulation-normal ovary without dominant foliculs.']",Figure 1 Sonography before induction of ovulation-normal ovary without dominant foliculs.,yes
PMC11460375,Figure_4,oa_package/2e/42/PMC11460375.tar.gz,"['In this case, a postoperative CT scan showed successful ablation of the liver and kidney lesions (A).', '(A-B): CT scan during the coaxiales needles control and after ablation.', '(A axial view) CT scan during the coaxiales needles control and after ablation (B axial view) with corresponding necrosis zones in liver segment VIII.', 'Notice the hemothorax (B) on the right basal side of the Lung (white arrow).', 'However, an active extravasation of contrast media adjacent to the right basal pleura was detected, resulting in hematothorax of the ipsilateral side (B).']",Fig. 4 (A-B): CT scan during the coaxiales needles control and after ablation. (Figure 4A axial view) CT scan during the coaxiales needles control and after ablation (Figure 4B axial view) with corresponding necrosis zones in liver segment VIII. Notice the hemothorax (Figure 4B) on the right basal side of the Lung (white arrow).,yes
PMC5200898,Figure_2,oa_package/60/ff/PMC5200898.tar.gz,['.'],"Fig. 2. (A) A normal, healthy kidney (left), and a magnified view of the structure of a tubule and its associated vasculature (right). (B) A chronically diseased kidney, showing the processes that lead to tubulointerstitial fibrosis. (C,D) Histological sections of an adult rat kidney, stained with Masson's trichrome (20 magnification; scale bars: 50m). (C) The glomerular and tubular architecture of a normal adult rat kidney, and (D) glomerulosclerosis (#) and tubulointerstitial fibrosis (*) in a 12-month-old hydroxysteroid dehydrogenase 2 ( )-knockout rat exhibiting end-stage renal disease.",yes
PMC10978067,Figure_1,oa_package/49/94/PMC10978067.tar.gz,['EKG on PresentationEKG: Electrocardiogram.'],Figure 1 EKG on Presentation EKG:Electrocardiogram.,yes
PMC8524180,Figure_3,oa_package/eb/c8/PMC8524180.tar.gz,"['Schematic for procedural steps of implantation.', '35]) ().']",Figure 3 Schematic for procedural steps of implantation.,yes
PMC8345115,Figure_10,oa_package/db/3d/PMC8345115.tar.gz,[],"Figure 10 SMLM of miR-21 aggregates in the nuclear area of a carcinoma cell within a human colorectal cancer tissue sample. Smaller blinking events were detected in the cytosolic area as well. ( ) Widefield and ( ) super-resolved reconstruction images. Two ROIs are highlighted in white ( , ) showing miR21 aggregates. Scale bars 3 m. Insets scale bars 200 nm.",yes
PMC11598987,Figure_5,oa_package/1e/23/PMC11598987.tar.gz,"[' shows the five most frequent malignant tumors in the six most common dog breeds.', 'The five most frequent diagnosis of malignant tumors in the six most common dog breeds with a diagnosis of malignancy.']",Figure 5 The five most frequent diagnosis of malignant tumors in the six most common dog breeds with a diagnosis of malignancy. ( ) German shepherd; ( ) Pinscher; ( ) Labrador retriever; ( ) Boxer; ( ) Beagle; ( ) Yorkshire terrier.,yes
PMC3193666,Figure_1,oa_package/9b/53/PMC3193666.tar.gz,"['Staining was considered diffuse if more than 50% of peritubular capillaries (PTC) were positive and focal if less than 50% peritubular capillaries were positive [].', '(a) Diffuse positivity in peritubular capillaries (C4d HRP Polymer, 100) (b) Focal positivity in peritubular capillaries (C4d HRP Polymer, 200)Grades of C4d positivity Grade 1 (a), Grade 2 (b) and Grade 3 (c) (C4d HRP Polymer, 100) (d) Glomerular positivity for C4d (C4d HRP Polymer, 200)The association of C4d immunostaining with various histological features was studied by 2 test using SPSS software.']","Figure 1 (a) Diffuse positivity in peritubular capillaries (C4d HRP Polymer, 100) (b) Focal positivity in peritubular capillaries (C4d HRP Polymer, 200)",yes
PMC10789373,Figure_52,oa_package/bf/e5/PMC10789373.tar.gz,[],Figure 3.4 Echocardiographic monitoring during trastuzumab therapy. Echo: echocardiography. GLS: global longitudinal strain; LVEF: left ventricular ejection fraction.,yes
PMC4557097,Figure_4,oa_package/1a/02/PMC4557097.tar.gz,['Cathepsin B gene deletion reduces neuronal loss after TBI.'],"Figure 4 . Quantitative image analyses of brain sections evaluated for Lesion Volume were also analyzed for neuronal cell density in the CA3 region of the hippocampus, which is distal to the impact site. TBI WT, but not TBI KO, mice had lower neuronal density than Sham WT and Sham KO animals. Thus, cathepsin B knockout resulted in reduced neuronal loss. Representative micrographs from the brains of Sham WT, Sham KO, TBI WT, and TBI KO animals are shown in , respectively (meanSEM, Bonferronis multiple comparison test <0.05, =10 animals/group, * TBI WT vs. Sham WT, Sham KO and TBI KO) ( ). Data from cited publication adapted for graphic display.",yes
PMC11296295,Figure_3,oa_package/ac/1d/PMC11296295.tar.gz,"['In some areas, there were also aggregates of eosinophilic granular cells (A-B).', '3Surgical procedureThe patient was planned for surgery under general anesthesia.']","Fig. 3 Photomicroscopic images of the lesion showing islands of epithelial cells with central edema and loose arrangement, bordered by a rim of palisading columnar cells. The morphology is consistent with ameloblastoma; H&E staining at 40 original magnification (A) and high magnification at 200 original magnification (B).",yes
PMC10008093,Figure_2,oa_package/8a/a3/PMC10008093.tar.gz,"['In all cases, confirmation should be made using CMR based on the modified Lake Louise criteria,43 which have elevated sensitivity and specificity in the acute phase for clinical features with considerable clinical suspicion (figure 2\n).', 'Examples of myocarditis after mRNA vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) confirmed with cardiac magnetic resonance imaging and endomyocardial biopsy.']","Figure 2 Examples of myocarditis after mRNA vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) confirmed with cardiac magnetic resonance imaging and endomyocardial biopsy. Involvement of the basal lateral face (arrow) meets T (A: prolongation of native T time; E: late enhancement) and T (B: prolongation of T time; C: hyperintense signal in T -weighted images) criteria and is visible in the cine sequence performed after contrast administration (D). Biopsy (F) shows mild lymphoplasmacytic inflammation with eosinophils, interstitial edema, and minimal necrosis of cardiomyocytes. Post-GD SSFP, steady-state free precession after administration of contrast agent (gadolinium); PSIR, phase-sensitive inversion recovery; STIR, short tau inversion recovery.",yes
PMC5091233,Figure_3,oa_package/12/7d/PMC5091233.tar.gz,"['Pathology slide with hematoxylin and eosin stain, consistent with diffuse large B-cell lymphoma.']","Fig. 3 Pathology slide with hematoxylin and eosin stain, consistent with diffuse large B-cell lymphoma.",yes
PMC11502597,Figure_6,oa_package/6a/f5/PMC11502597.tar.gz,"[' 6), Scharnierbr che der Sch delbasis (bei beidseitiger stumpfer Gewalteinwirkung, z.', '6Postmortale Computertomographie (PMCT), cinematographische 3D-Rekonstruktion eines Globusbruchs mit Biegungsbr chen (rote Umkreisung) und Berstungsbruch (gr ner Pfeil) infolge Sturz auf den HinterkopfAbb.']","Abb. 6 Postmortale Computertomographie (PMCT), cinematographische 3D-Rekonstruktion eines Globusbruchs mit Biegungsbrchen( ) und Berstungsbruch( ) infolge Sturz auf den Hinterkopf",yes
PMC9411153,Figure_1,oa_package/13/37/PMC9411153.tar.gz,"['Magnified view of a tissue patch (right) extracted from one core (middle) of a TMA (left) from the TNBC cohort.', 'Deep learning has gained recognition as a method of developing fast and accurate computational models to perform complex real-world tasks at the same level of performance as an expert human in that field.', '1) was acquired from St.']",Figure 1 Magnified view of a tissue patch (right) extracted from one core (middle) of a TMA (left) from the TNBC cohort. On average each core is 1.25 mm in diameter. All slides were scanned at 0.25 m/pixel resolution.,yes
PMC9733150,Figure_3,oa_package/b2/45/PMC9733150.tar.gz,"['A repeat CT scan of the abdomen and pelvis with intravenous, oral, and rectal contrast () showed findings concerning for a 2.', '.']","Figure 3. Computed tomography of the abdomen and pelvis with intravenous, oral, and rectal contrast showing reduction of intussusception and an arrow pointing to the intra-luminal mass within the sigmoid colon.",yes
PMC9929938,Figure_1,oa_package/d9/cb/PMC9929938.tar.gz,"['Aortic dissection (AD) () is among the most serious complications of TAA, and while modern CT angiography provides nearly perfect detection (15), reliable availability of contrast-enhanced imaging is not universal in some settings.', 'Volume rendering of an acute Type A aortic dissection as seen on CTA.']",Figure 1 Volume rendering of an acute Type A aortic dissection as seen on CTA.,yes
PMC11085018,Figure_5,oa_package/22/10/PMC11085018.tar.gz,"['A brain MRI was performed as part of the epilepsy investigation, which showed thickening of the right frontal and occipital bones, as well as the bones of the facial skeleton, along with exophthalmos and meningeal enhancement ().', '1177_2333794X241251746-fig5"" position=""float""/>.']","Figure 5. Sagittal and axial sections showing bone thickening of the right frontal, occipital, and right facial bones with iso-signal on T1 (a and b), heterogeneous hyperintensity on T2 Flair (c), enhancing heterogeneously after contrast injection with enhancement (d).",yes
PMC10386209,Figure_2,oa_package/0a/b3/PMC10386209.tar.gz,"[' 2A) and ~ 100 150 mV/mm in the cortex (', ' 2G).', '\n\nGES decreased A 42 and A 40 in the hippocampus and cortex of 5xFAD mice\nA, G.', 'sham\nAfter the 4-week stimulation program (', ' 2B H).', ' 2C, D and I J).', '( 2E F and 2 K-2 L).']","Fig. 2 EF distribution to the hippocampus ( ) and cortex ( ) simulated by the FEM. Following the 4-week GES, the hippocampus ( , red box) and cortex tissues ( , red box) of 5xFAD mice from each group were separately collected for A42/ A40 ELISA assay and immunofluorescence microscopy. ELISA assay showed that the GES treatment significantly decreased A42 and A40 concentrations in the hippocampus ( ,) and the cortex ( ) following GES in 25, 50, 100, and 200 A groups. Typical example immunofluorescence images show significantly decreased A42 (red in and ) and A40 (red in and ) labeling in the dentate gyrus (DG) of the hippocampus ( ) and in the cortex ( ) following 200 A GES. NeuN (green) and DAPI (blue) were used to label neurons and nuclei. Scale bars: 50m Data were presented as meanSEM. * P<0.05, ** P<0.01, and *** P<0.001 were considered as significantly different for GES groups vs. sham",yes
PMC10680640,Figure_7,oa_package/8e/d9/PMC10680640.tar.gz,"['Consistent with the previous studies, the protein levels of lysosomal enzymes Cathepsin D (CathD) and Cathepsin L (CathL) were slightly increased in the brain lysates of Tmem106b / mice compared to the WT mice [29], but it was not altered in PS19 mice (Supplementary A and 7B).', 'Levels of CathD and CathL are not further increased in the brain lysates of Tmem106b / PS19 mice compared to Tmem106b / mice (Supplementary A and 7B).', 'However, this decrease was not observed in Tmem106b / PS19 mice (A and 7B).', 'p62 level was not altered in the sarkosyl-insoluble fraction in all the mice analyzed (A and 7B).', '5-month-old Tmem106b / PS19 mouse brain when compared to PS19 mice (A and 7B), with slight upregulation seen in PS19 mice compared to WT mice, but no changes are detected in sarkosyl-soluble fractions(', '5-month-old Tmem106b / PS19 mice (C).', '5-month-old Tmem106b / PS19 mice compared to PS19 mice, but the the increased LC3 signals were not colocalized with CathD in CA3 neuronal soma (D and 7E),TMEM106B ablation upregulates lysosomal CathD in microglia through TFE3/TFEB in PS19 miceTo further determine the sources of the CathD-positive clusters in the CA3 region (', 'Consistently, we found increased accumulation of p62 and ubiquitinated proteins in the brain of Tmem106b / PS19 mice and co-localization between hTau aggregates, p62 and ubiquitinated proteins (A C), indicating that loss of TMEM106B results in impaired degradation of hTau and ubiquitinated substrates via autophagy-lysosome pathway.', '566707v1-f0006"" position=""float""/>.']","Figure 7. Genetic deletion of TMEM106B upregulated the level of p62 and ubiquitinated proteins in PS19 mice Western blot analysis of p62 and Ub in sarkosyl-soluble fraction and sarkosyl-insoluble fraction extracted the brain of 8.5-month-old WT, , PS19, and PS19 mice. GAPDH was used as the internal control in the sarkosyl-soluble fraction. The relative level of indicated proteins was quantified in B. n=46. Data presented as mean SEM. One-way ANOVA tests with Bonferronis multiple comparisons: *, p<0.05; **, p<0.01; ***, p<0.001. Representative image of Ub, p62, and hTau co-immunostaining in CA1 regions in the hippocampus of 8.5-month-old PS19 and PS19 mice. Scale bar: 20 m. Representative image of LC3 and hTau co-immunostaining in CA3 regions in the hippocampus of 8.5-month-old PS19 and PS19 mice. The relative fluorescence intensity of LC3 in CA3 somas was quantified in E. n=34. Data presented as mean SEM. Unpaired Students t-test: **, p<0.01. Scale bar: 20 m.",yes
PMC10468306,Figure_1,oa_package/45/39/PMC10468306.tar.gz,"['Large portions of the head, as well as the left side of the neck, exhibited extensive alopecia and severe multifocal ulcerative and purulent dermatitis, occasionally accompanied by partially detachable dark brown crusts up to 1 cm thick (a).', 'Additionally, there was a prolapse of the rectum (b) as well as an invagination in the colon involving 3 cm of the large intestine with venous infarction of the invaginated part (intussusceptum).', '.', 'Three additional affected kittens from the following litters showed similar dermatological lesions as the first kitten (c and d).', 'It is also possible that the rectal prolapse (b) represented a consequence of the collagen disturbances due to dermatospraxis EDS but might also have resulted from the colo-colic intussusception.']","Fig. 1. Gross condition of the deceased affected kittens (a and b initial case; c and d from second and third litter). a) Almost the entire head and the left neck showed extensive alopecia and severe multifocal ulcerative and purulent dermatitis occasionally accompanied by barky, dark-brown crust formation (arrow). The oral mucosa was moderately anemic. b) A rectal prolapse was also present in the initial case (arrow). c) After surgical treatment: severe loss of the epidermis especially in the cranial body regions (head, neck, forelimbs) with severe ulcerative partly crustose dermatitis and fragile skin, that tore at light touch (arrow); d) Kitten with a milder course, gross lesions were found exclusively on the head (temples and mucocutaneous junctions) with mild to moderate ulceration and crusting (arrow).",yes
PMC10653039,Figure_7,oa_package/6c/e7/PMC10653039.tar.gz,"['Notably, hypoxic regions consistently appeared outside of the lesions, in the parenchyma ( 7A).', ' 7.', ' 7To investigate if larger lesions resulted in more hypoxia and how far hypoxia extended from lesions, we cleared the brains of wild-type and Ccm3-iECKO mice ( 7C and supplemental Movies 1 and 2).', 'Hypoxia only appeared near the lesions of Ccm3-iECKO mice ( 7C, lower panel, and 7D).', 'We selected 262 random lesions from 3 Ccm3-iECKO mice and measured their lesion area, the associated hypoxic area, and the furthest hypoxic distance from the lesion ( 7D, right panel).', 'The graph in 7D shows the fluorescence intensity of hypoxyprobe (gray value) along the indicated blue line drawn across the lumen of the lesion.', 'The fluorescence intensity of hypoxyprobe (y-axis) and the lesion distance (x-axis) for the 262 lesions were plotted ( 7E).', '49, 7Fi) and lesions 5000 to 10 000 m2 wide gave a maximum mean AU of 2.', '20 ( 7Fii).', '49, 7Fiii), and the group with the largest lesions ( 50 000 m2) resulted in a different hypoxic pattern with a maximum hypoxyprobe signal of 2.', '0 AU at a 40- m distance from the lesion ( 7Fiv).', 'The lesion areas and their corresponding hypoxic areas were plotted and a moderate positive correlation was seen ( 7G).']","Figure6. (A) Kinetic expression of thrombomodulin at P6, P7, and P8 in the cerebellum (cb) shows thatthrombomodulin expression decreased in wild-type mice (red line) but remained high in mice (blue line). Thrombomodulin was significantly higher in at P7 ( = .0190). and P8 ( = .0043) when compared with wild-type mice. (B) Representative images of the cerebellum of wild-type (upper panel) and (lower panel) P8 mice stained with isolectin (green) and thrombomodulin (red). A merged image is shown in the right panel. (C) Images of the heterogeneous expression of erythrocytes (green) and thrombomodulin (red) show how (i) lesions filled with erythrocytes have a lower expression of thrombomodulin, whereas lesions that are hallowed (ii) and (iii) have a high expression of thrombomodulin. (D) Images showing the heterogeneous expression of VWF (green) and thrombomodulin (red) showing how some regions express high levels of vascular and extracellular VWF with thrombomodulin (dashed box, split channels to the right), and other vessels express low levels of VWF (asterisks). White arrows highlight secreted VWF, and yellow arrows highlight thrombomodulin/VWF colocalization. In panel A, the mean of n= 12 mice in each group are shown. Bars indicate the standard deviation in each group. A Mann-Whitney test was used to compare wild-type mice with mice; the corresponding values are indicated on each graph.",yes
PMC10544197,Figure_5,oa_package/2a/89/PMC10544197.tar.gz,"['Although they were not specific for increased BBB permeability, we identified several abnormalities across all 4 patients ().', 'We also observed hyperintensities in the bilateral putamen with a gradation pattern showing greater intensity in the anterior putamen fading into the posterior ().', 'MRI analysis of BBB breakdown in individuals with ASLD.']","Figure 5 MRI analysis of BBB breakdown in individuals with ASLD. Comparison of neuroimaging findings among patients with ASLD. Abnormal hyperintensities are best seen on axial T2 FLAIR MRI for patient 1, patient 2, patient 3, and patient 4. Bilateral hyperintensities can be seen in the putamen, globus pallidus, heads of the caudate nuclei, and insular cortices. A gradation pattern (anterior to posterior) of hyperintensity can be seen in the bilateral putamen (arrow).",yes
PMC10602106,Figure_3,oa_package/9f/6d/PMC10602106.tar.gz,"['To investigate further whether the amphipathic domain of -synuclein is responsible for the interaction with anionic nanoplastic and the promotion of -synuclein aggregation, we created two truncated -synuclein proteins, one with half of the amphipathic domain removed and the other with the entire domain deleted (A).', 'Our results show that without the amphipathic domain, the truncated proteins fail to interact with the anionic nanoplastic (B).', 'Although the truncated variants aggregate faster than the full-length -synuclein protein, the addition of anionic nanoplastic particles did not significantly stimulate their aggregation (C), indicating the necessity of the amphipathic domain both for binding and for interaction in aggregation.', 'Full-length human -synuclein monomer has an exceptionally high affinity of ~2 nM Kd with anionic nanoplastic at physiological buffer and pH conditions (D).', '.']","Fig. 3. Anionic nanoplastic tightly binds to the -synuclein amphipathic domain to initiate fibrillation A) Domain structure of -synuclein. Aligned truncated variants in green (36140) and purple (60140) are aligned to full-length protein in gray (1140). B) Column graphs showing the percentage of full-length, or truncated, monomeric -synuclein binding to nanoplastic particles (anionic or cationic). Dynamic light scattering (DLS) resolves complexes at a stoichiometry of 10:1 protein to nanoplastics. Bars represent mean values of 20 independent acquisitions (SEM shown). *p<0.01, ****p<0.0001, one-way ANOVA with Dunnets post-hoc test. C) Spontaneous full length or truncated -synuclein aggregation assessed through thioflavin-T (ThT) fluorescence over time, with or without 2.5 nM anionic nanoplastic particles as indicated. Curves represent mean values from quintuplicate independent reactions (SEM shown). Black dashed horizontal line indicates 10% ThT maximum fluorescence used to calculate threshold (C ). Bar graph shows mean fold change from control with nanoplastic addition (C ). Means are from quintuplicate independent reactions (SEM shown). ****p < 0.0001 from one-way ANOVA with Tukeys post-hoc test. D) Representative surface plasmon resonance (SPR) sensorgrams with full length -synuclein (1140, left panel), truncated 36140 (middle panel), or 60140 (right panel), with a range of nanoplastic contaminants in solution. Sensorgrams are globally fit to heterogeneous ligand models repeated in triplicate for each protein variant. Average K and X values (1140, full length protein), or estimated values (low confidence X for the truncated proteins), are shown from three independent array runs (SEM indicated).",yes
PMC4266037,Figure_9,oa_package/b8/7b/PMC4266037.tar.gz,"['.', ' Photomicrographs from kidneys, liver and pancreas from the same patient shown in figure 9.']","Figure 9. Nonobstructive renal dysplasia-polysplenia syndrome. (A) The only apparent body anomaly was a distended abdomen, but the child did not have a hypoplastic thorax. (B) There were several small splenicula at the left upper quadrant. The liver was moved up to allow better visualization and the forceps are holding the splenicula. (C) Both kidneys were dysplastic; however, the ureters were of normal caliber and the renal pelvis was not dilated. (D) Close-up view from the left kidney discloses lack of demarcation between cortex and medulla and several small cysts scattered throughout the parenchyma.",yes
PMC5651883,Figure_1,oa_package/e7/54/PMC5651883.tar.gz,"[' 1a), characterized by few regenerative fibers (Supplementary ', ' 1b and Supplementary ', ' 1b and Supplementary ', 'Lack of Nfix improves signs of muscular dystrophy.', ' 1 for the analysis of central nucleation in tibialis anterior and diaphragm muscles at 3 and 5 weeks\nMuscular dystrophy amelioration in absence of Nfix persists up to 6 months.']",Fig. 1 Lack of Nfix improves signs of muscular dystrophy. Hematoxylin and eosin (H&E) and Milligans trichrome staining of tibialis anterior (left) and diaphragm (right) muscles at 3 weeks of age; =12 null and 6 null: null mice. Scale bar 100m. Hematoxylin and eosin (H&E) and Milligans trichrome staining of tibialis anterior (left) and diaphragm (right) muscles at 5 weeks of age; =5 null and 5 null: null mice. Scale bar 100m. See also Supplementary Fig. for the analysis of central nucleation in tibialis anterior and diaphragm muscles at 3 and 5 weeks,yes
PMC6083599,Figure_4,oa_package/d1/c0/PMC6083599.tar.gz,"['003""/>\nUltrastructure of preimplantation kidney biopsy from deceased donor exposed to Takotsubo cardiomyopathy (TCM).']","Figure 4 Glomerular endothelial cells are swollen, disintegrated, and partly detached from glomerular basement membrane. Swollen endothelial cell and extracellular debris fill the lumen of glomerular capillary. Swollen endothelial cell in peritubular capillary. Their ytoplasmic membrane forms endothelial projections (microvilli). Tubular epithelial cells are preserved but attenuated. Desmosomes are clearly seen. Apical brush border is thinner. Arteriole with swollen and partly detached endothelial cells.",yes
PMC10450110,Figure_9,oa_package/45/95/PMC10450110.tar.gz,"['This undue retraction and lack of proper exposure often lead to undue handling of the already friable and inflamed tissues in the right iliac region, leading to further post-operative complications like faecal fistula[1920] [].', 'Fistulogram showing the fistulous tract of faecal fistula in a case of complicated appendicitis operated elsewhere using a McBurney s incision in an 8 years old boy.']",Figure 9 Fistulogram showing the fistulous tract of faecal fistula in a case of complicated appendicitis operated elsewhere using a McBurneys incision in an 8 years old boy. The fistula had been present for 2 years at the time of presentation at our center. Complete excision of the fistulous tract with repair of caecal wall was done with omental patching. 1. Fistulous tract extending from skin to the caecum. 2. Collection of contrast seen in the caecum,yes
PMC3339103,Figure_3,oa_package/8c/16/PMC3339103.tar.gz,"[' highlights our IHC results, including the compartmentalization of vimentin around several skin appendices where the DIF autoreactivty was detected.', '(DIF in b, c and d, H E in a, e, g and bleached H E in h, IHC in f) .']","Fig. 3 . Some alterations in dermal sweat glands (black arrows), and their respective nerves (red arrows), under the area where the viral infection was detected in the epidermis. . Positive staining of the sweat glands (white arrows, green staining) and the nerves (red arrows, green staining with FITC conjugated anti human IgG and C3 -FITC antibodies). . The positive nerve, at higher magnification (green staining, red arrow). . Similar to and , but in this case, to confirm the identity of the nerve, we co-localized the nerve by staining with Cy3 conjugated anti-human glial fibrillary acidic protein (GFAP)(white staining, red arrow). A nearby sweat gland is indicated by the white arrow. . Some rarefaction of the sweat glands on H&E at higher magnification. . IHC positive staining of Complement/C5-C9/MAC on sweat glands (brown staining; black arrow). and . Melanin bleaching confirmed the presence of melanin in those pigmented areas (H&E and melanin bleaching, respectively; black arrows). Shows the clinical generalized vesicles and bullae on the upper arm (black arrow).",yes
PMC3683175,Figure_1,oa_package/37/1c/PMC3683175.tar.gz,"['[50] Whereas classic chordoma displays typical physaliphorous cells [], chondroid chordomas exhibit typical chordoma architecture with areas of cartilaginous hyaline stroma,[34] but with positive cytokeratin and epithelial membrane antigen immunoreactivity.', '[15]Histologic specimen stained with hematoxylin and eosin stain demonstrating the classic lobular architecture of chordoma, 10 (a), composed of physaliphorous cells, 60 (b)Systemic metastases may occur in as many as 30% of patients,[1426] although they tend to be more frequent in sacral than skull base chordomas.']","Figure 1 Histologic specimen stained with hematoxylin and eosin stain demonstrating the classic lobular architecture of chordoma, 10 (a), composed of physaliphorous cells, 60 (b)",yes
PMC8253882,Figure_1,oa_package/d1/6d/PMC8253882.tar.gz,"['Physical examination demonstrated a tightly adherent, thickened, and hyperpigmented plaque over the anterior aspect of the right shin ().', 'Hyperpigmented, thickened, and sclerotic plaque over the right shin.']","Fig 1 Hyperpigmented, thickened, and sclerotic plaque over the right shin.",yes
PMC9228914,Figure_1,oa_package/84/d5/PMC9228914.tar.gz,"['(A) CT scout view (topogram, instead of X-ray kidney, ureter and bladder) revealing diffuse alveolar calcification with kidney shape in right renal region and a well-positioned pigtail catheter; (B) computed tomography image showing the densely calcified right kidneyCite this article: Savas Deftereos et al.']","Figure 1 (A) CT scout view (topogram, instead of X-ray kidney, ureter and bladder) revealing diffuse alveolar calcification with kidney shape in right renal region and a well-positioned pigtail catheter; (B) computed tomography image showing the densely calcified right kidney",yes
PMC7550887,Figure_3,oa_package/56/f8/PMC7550887.tar.gz,"['A chest radiograph demonstrated bilateral parenchymal opacities which can be seen with COVID-19, as well as extensive subcutaneous emphysema, pneumomediastinum, and trace apical pneumothoraces (\n).', 'Portable radiograph of Patient 3.', '']",Fig. 3 Portable radiograph of Patient 3. Bilateral peripheral airspace opacities are suggestive of COVID-19 infection. There was suspicion for pneumomediastinum given a continuous diaphragm sign (white arrow) and associated subcutaneous emphysema (black arrow).,yes
PMC9492358,Figure_6,oa_package/47/6d/PMC9492358.tar.gz,"['The tumor cells were polygonal, with pale to amphophilic cytoplasm, as well as round, deeply stained nuclei with nuclear pleomorphism ().', '005"" position=""float""/>Photomicrograph of left adrenal tumor of Patient B.', '(a) Tumor cells with round nuclei and finely vacuolated cytoplasm that were arranged in anastomosing cords with delicate vasculature, resembling the previous left adrenal tumor () (H E 400).']",Figure 6 Photomicrograph of left adrenal tumor of Patient B. (a) Tumor cells in trabecular pattern (H&E100). (b) Tumor cells demonstrate nuclei of variable size and eosinophilic granular cytoplasm (H&E400).,yes
PMC4636375,Figure_2,oa_package/0e/e5/PMC4636375.tar.gz,"['g002MCV sT induces hyperproliferaton of acral skin.', '1% (11/18) of Ubc\nCreERT2; ROSA\nsT mice (A).', '8%, 9/11) ().']",10.1371/journal.pone.0142329.g002,yes
PMC4265693,Figure_2,oa_package/47/f0/PMC4265693.tar.gz,"[""We had a recent case in a young woman with fatal Goodpasture's syndrome showing diffuse pulmonary hemorrhage and hemosiderosis ()."", ""The deceiving factor in our current case with microscopic polyangiitis of lung and the Goodpasture's syndrome presented in was the fact that renal function appeared relatively normal in both patients during the early course of their disease."", '001""/>Goodpasture\'s syndrome in a 26-year-old woman.']","Figure 2 Goodpasture's syndrome in a 26-year-old woman. The patient presented with rapid progressive dyspnea over 2 weeks. Twice bronchoalveolar lavages showed hemosiderin-laden macrophages in the cytology specimens. Later she was found to have positive serum level for antiglomerular basement membrane antibody, although her renal function was not obviously compromised. The patient expired despite intensive care. Microscopically, there was diffuse alveolar hemorrhage and hemosiderosis in the bilateral lung sections at the autopsy (panel (a), 100, and panel (b), 400). The hemosiderosis was confirmed by diffuse positive iron staining in hemosiderin-laden macrophages in panel (c) ((c), 400). In panel (d), glomeruli were positive for linear IgG staining on immunofluorescent section, confirming antiglomerular basement membrane disease ((d), 400), but no crescent formation was identified in the glomeruli. Overall autopsy findings were consistent with Goodpasture's syndrome with dominant pathologic changes in her lungs.",yes
PMC4890852,Figure_4,oa_package/ec/06/PMC4890852.tar.gz,"['As shown in A, NMNAT2 complexes with HSP90 but not HSP70, HOP or CHIP in NMNAT2-transfected HEK293-tau cells, via its C-terminus (S6 Fig) in rTg4510 cortex (B) but also bind to hTau (B).', 'g004NMNAT2 complexes with HSP90 to refold aggregated proteins.', 'ref082"" ref-type=""bibr"">82] (C).', 'We found that decreasing HSP90 function severely impaired NMNAT2 s ability to reduce p-hTau (D; p 0.', 'The cells were transfected with Luciferase and NMNAT2-WT together with scrambled or HSP90 siRNA (E).', '001) prevented NMNAT2 s facilitation of the recovery of aggregated luciferase, but these treatments have a minimal impact on NMNAT2 s holdase activity (E).', 'Hence, reduction or inhibition of HSP90 promotes the heat shock response (C, Localization and distribution of viral antigens and lesions in Class-IA and Class-IB brains.']","Figure 5 Localization and distribution of viral antigens and lesions in Class-IA and Class-IB brains. Representative images of mouse brain coronal sections marked to demonstrate the localization of viral antigens ( ) and pathological lesions ( ) observed in the brains of infected mice. Larger dots represent more intense signals or greater lesion size. Shown are the cerebellar cortex ( ) ( ); hypothalamus ( ) ( , ); hippocampus ( ) ( , ); thalamus ( ) ( ); midbrain ( ) and pons ( ) ( ); and cerebellum ( ) and medulla oblongata ( ) ( ). Templates of coronal sections were downloaded from and .",yes
PMC2849938,Figure_2,oa_package/d9/63/PMC2849938.tar.gz,"[' 2a, b, black arrows).', ' 2c, d).', 'Scale bar 33 m\nThe presence of intraneuronal A in the hippocampal region of sporadic AD cases was confirmed qualitatively by staining using the OC antibody recognizing A fibrils and fibrillar oligomers [32].']","Fig.2 Double labeling of A in (DAB) using A[N] antibody and astrocytes in (Histogreen, ) using a GFAP antibody in paraffin-embedded sections. The highly granular staining pattern surrounding the smaller nuclei was due to astrocytes accumulating A and was found in CA4 ( ) as well as in CA3 ( ) of many sporadic AD cases. However, in some AD cases, astrocytes without granular staining were found close to neurons in both CA4 ( ) and CA3 ( ). 33m",yes
PMC10613125,Figure_3,oa_package/fa/37/PMC10613125.tar.gz,[],"FIGURE 3 Palmitate induces insulin resistance in vitro in oligodendrocytes. (a) Human oligodendrocytes were treated with control (BSA) or palmitate (150M) for 48h followed with/without insulin (20nM) for 30min and cell lysates were analysed by WB for pAkt and pERK. The same blots were stripped and reprobed for total Akt and ERK, and tubulin. Relative levels from WB analysis of (b) pAkt versus total Akt and (c) pERK versus ERK in control versus palmitatetreated oligodendrocytes. Data are presented as meanSEM from at least three separate experiments. ** <0.01, by Student's test.",yes
PMC3170936,Figure_1,oa_package/23/fb/PMC3170936.tar.gz,[],"Fig. (1) A3D brain imagesof the same subject are acquired using CT (left), MR (middle) and PET-FDG (right).",yes
PMC5139081,Figure_8,oa_package/dd/eb/PMC5139081.tar.gz,"[' 8).', 'CF airway epithelium and pathogen adaptation.', 'aeruginosa also releases outer membrane vesicles containing CF inhibitory factor (Cif), a protein that further inhibits the recycling of CFTR in the host further contributing to the cycle of hyper-inflammation and bacterial colonization\nNeutrophils also use their extracellular host defense armamentarium, also known as neutrophil extracellular traps (NETs) [162] and the intracellular stored anti-microbial effector proteins such as defensins and proteases for killing pathogens.']","Fig. 8 CF airway epithelium and pathogen adaptation. Defective CFTR leads to decreased airway surface liquid (ASL) layer. This facilitates microbial colonization and airway inflammation. Pathogen-associated molecular patterns (PAMPs) activate Toll-like receptor (TLR) signaling to activate Interleukin-8 (IL-8) and therefore to recruit polymorphonuclear neutrophils (PMNs). The increasing PMNs result in oxidative stress within the airways by forming reactive oxygen species (ROS). The increased oxidative stress further activates the mitogen activated protein kinase pathway, activating IL-8 and thus recruiting more PMNs. Mutated CFTR in the epithelial cells is unable to channel the antioxidants Glutathione (GSH) and thiocyanate (SCN ) into the airway, limiting their ability to counteract the oxidative stress. TLR expression and signaling is also altered in CF epithelium. Expression of TLR2 and TLR5 at the apical surface is increased, whereas TLR4 expression is limited to endosome (not shown here). NF-B in CF airway epithelial cells is constitutively activated, resulting in the production of inflammatory cytokines including IL-8 and granulocyte macrophage colony stimulating factor (GM-CSF). This also leads to recruitment of PMNs independent of TLRs interaction with the adaptor protein MyD88. Bacterial PAMPs further increase NF-B signaling through activation of TLR-MyD88 signaling. The inhaled bacteria start interacting and aggregating to form biofilms. also releases outer membrane vesicles containing CF inhibitory factor (Cif), a protein that further inhibits the recycling of CFTR in the host further contributing to the cycle of hyper-inflammation and bacterial colonization",yes
PMC10649397,Figure_2,oa_package/62/fb/PMC10649397.tar.gz,"['This results in pre-sinusoidal dilation, decreased hepatic artery blood flow, and decreased arterial oxygen saturation, which can ultimately lead to irreversible congestive liver fibrosis and cardiac cirrhosis ().', 'Physiopathological changes in congestive hepatopathy.']","Figure 2 Physiopathological changes in congestive hepatopathy. CH is characterized by pre-sinusoidal dilation, decreased () hepatic artery blood flow and decreased () arterial oxygen saturation, which can ultimately lead to irreversible congestive liver fibrosis and cardiac cirrhosis. Key to the colors used: green for bile ducts, red for hepatic arterial vessels, purple for portal vessels. CH: Congestive hepatopathy.",yes
PMC11618731,Figure_4,oa_package/54/14/PMC11618731.tar.gz,"[' shows histologic characteristics of the lesion resected in Case 1.', ':Histopathology of primary intracranial sarcoma in Case 1.']","Figure 4: Histopathology of primary intracranial sarcoma in Case 1. (a) Intracranial sarcoma showing pleomorphic features and prominent vasculature. Hematoxylin and eosin (H&E) stain 20 objective, 200 augments; (b) cytoplasmic hyaline globules in pleomorphic sarcoma. H&E stain 40 objective, 400 augments; (c) atypical multipolar mitosis and marked nuclear anaplasia with the presence of cytoplasmic hyaline globules. H&E stain 40 objective, 400 augments; and (d) intracranial sarcoma showing prominent vasculature and tumoral cells cytoplasmic vacuolization. H&E stain 20 objective, 200 augments.",yes
PMC11424899,Figure_3,oa_package/91/39/PMC11424899.tar.gz,"['MRI of the nasopharynx revealed a confined nasopharyngeal mass centered in the left Rosenm ller fossa with intermediate signal on T1,T2 weighted images and high signal intensity on Fat-satT2-weighted image, it presented intense and homogeneous enhancement on Fat-sat T1-weighted images ().', '(A) and (B) Axial fat-sat T2 weighted and axial fat-sat postgadolinium T1-weighted images, (C) Coronal T2 weighted image, (D) Sagittal T1 weighted image.', ':The patient received exclusive radiotherapy, with a total dose of 50 Gy delivered in 25 fractions of 2 Gy each.']","Fig. 3 (A) and (B) Axial fat-sat T2 weighted and axial fat-sat postgadolinium T1-weighted images, (C) Coronal T2 weighted image, (D) Sagittal T1 weighted image. Confined nasopharyngeal mass centered in left Rosenmller fossa with high signal intensity on T2, It demonstrated intense and homogeneous enhancement (arrowhead).",yes
PMC9101064,Figure_5,oa_package/33/35/PMC9101064.tar.gz,"['All fractures were classified by the study coordinator as incomplete/buckled (discontinuous fracture line), dislocated (offset bone width), or undislocated (offset bone width with continuous fracture line; ).', 'Fracture patterns.']","Figure 5 Fracture patterns. Fractures were classified by the study coordinator as incomplete/buckled ( ), undislocated ( ), or dislocated ( ).",yes
PMC2808388,Figure_6,oa_package/b4/1d/PMC2808388.tar.gz,"['Expression was transient, so by 8 weeks, only 2 out of 5 animals showed few MHC II cells in SNc (Table 1 A).', 'In animals of the -syn-neurodegeneration group, we observed at 4 weeks a significant robust up-regulation of MHC II expression in the ventral midbrain, where the area immunostained for MHC II covered the full SN (both SNc and SNr) (Table 1 A).', 'The MHC II cells remained numerous throughout the SN at 8 weeks, then decreased and became restricted to SNc at 15 weeks (Table 1 A).', 'Expression remained elevated at the level of the SNc in 3 out of 5 animals at 15 weeks (Table 1 A).', 'g006MHC II microglial expression.', 'g006""/>It is interesting to note that we observed round small cells, which stained positive for MHC II (C).', 'In addition, at 4 8 weeks, we observed MHC II cells associated with and within blood vessels in both -syn groups (D E).']",10.1371/journal.pone.0008784.g006,yes
PMC8379469,Figure_3,oa_package/4c/f8/PMC8379469.tar.gz,"['In the present study, the R6/2 (CAG 242-257) mice display a significant loss of the number of orexin ( 3A), MCH ( 3B) and CART ( 3C) neurons localized in the lateral hypothalamic area, in addition to the loss of oxytocin neurons in the PVN ( 3D) at 12 weeks of age.', 'The vasopressin-positive cell number is significantly reduced in R6/2 mice only at 6 weeks of age ( 3F), while the number of A13 TH-positive neurons is comparable between R6/2 (CAG 242-257) mice and controls at both 6 and 12 weeks ( 3E).', ' 3Stereological assessment of hypothalamic populations in the R6/2 mouse model expressing 242 257 CAG repeats in 6- and 12-weeks old mice.']","Figure3 Stereological assessment of hypothalamic populations in the R6/2 mouse model expressing 242257 CAG repeats in 6- and 12-weeks old mice. The number of (A) orexin (n = 6/genotype, p = 0.0260, Mann-Whitney test), (B) MCH (n = 6/genotype, p = 0.0022, Mann-Whitney test), (C) CART (n = 6/genotype, p = 0.0022, Mann-Whitney test) and (D) oxytocin (n = 6/genotype, p = 0.0043, Mann-Whitney test) positive cells were reduced at 12-week-old R6/2 mice compared to their WT littermate controls. (E) The number of A13 TH positive cells was comparable at both 6 and 12 weeks. (F) The vasopressin expressing cell numbers were significantly reduced in R6/2 mice compared to WT littermates at 6 weeks (n = 10/genotype, two-tailed unpaired t-test, p = 0.0341). (F) Representative photomicrographs of hypothalamic sections processed for orexin, MCH, CART, oxytocin, TH, and vasopressin immunohistochemistry in 12 weeks old WT and R6/2 mice. Data are represented as scatter dot plots, and bars represent mean SEM. Scale bar = 200 m.",yes
PMC6305935,Figure_7,oa_package/29/58/PMC6305935.tar.gz,"['Red: left right, green: anterior posterior, blue: superior inferiorStructural connectivity correlated with tau deposition in orbitofrontal lobe.']","Figure 7 Structural connectivity correlated with tau deposition in orbitofrontal lobe. The CN group shows decreased connectivity (FDR=0.0064), whereas the ADspectrum group shows increased connectivity (FDR=0.0017). Red: leftright, green: anteriorposterior, blue: superiorinferior",yes
PMC5977127,Figure_3,oa_package/c7/60/PMC5977127.tar.gz,"[' demonstrates active extravasation in the arterial and delayed phases.', 'Axial images of active extravasation of contrast into a medial left thigh hematoma following traumatic injury to the left leg: (a) Small volume of contrast extravasation seen during the arterial phase (arrow); (b) Increase in size of the collection of extravasated contrast in the delayed phase imaging (arrow).']",Figure 3 Axial images of active extravasation of contrast into a medial left thigh hematoma following traumatic injury to the left leg: ( ) Small volume of contrast extravasation seen during the arterial phase (arrow); ( ) Increase in size of the collection of extravasated contrast in the delayed phase imaging (arrow).,yes
PMC6667427,Figure_1,oa_package/e2/aa/PMC6667427.tar.gz,"[' 1, Online Table 1).', '99mTc-nanocolloid SPECT/CT in sentinel node lymphoscintigraphy.', 'New York: Springer; 2017:1363 1400]Similarly, SLN mapping has been validated in patients with intermediate-thickness melanoma.', ' 11), CT information helps to obtain a more robust spatial normalization of SPECT data [232 234].', '1123I-FP-CIT SPECT/CT in a patient with parkinsonism.', 'Duccio Volterrani, Regional Center of Nuclear Medicine, University of Pisa, Italy]Gastrointestinal tractActive gastrointestinal (GI) bleeding is detected by contrast angiography or endoscopy if present at the time the study is performed.', ' 12) [247].', '2Bone SPECT/CT diagnosis of spondylolysis in an adolescent patient.', 'Delayed WB-BS, anterior and posterior views (b) show a focus of abnormal 99mTc-MDP uptake in left aspect of L5 vertebra, localized by SPECT/CT (c) to the left L-5 articular facet, consistent with spondylolysisIn paediatric solid tumours, SPECT/CT is used mainly with neuroblastic tumours and thyroid cancer.', ' 13) and reduces the incidence of equivocal planar findings [250].', '3123I-mIBG SPECT/CT in neuroblastoma.', 'SPECT/CT (b) localizes this abnormal tracer uptake to the primary lesion in a large mass in the right adrenal seen on CT (C)DTC, although uncommon, has a rising incidence in children who tend to have a more aggressive type as compared to adults [252].']","Fig. 1 Tc-nanocolloid SPECT/CT in sentinel node lymphoscintigraphy. Preoperative sentinel lymph node (SLN) mapping in a 63-year-old woman with cancer of the left breast following intratumoral injection of Tc-nanocolloidal albumin. Planar imaging with body contouring ( ), with a Co flood source beneath the patients body, visualizes lymphatic drainage to SLNs in the left axilla, periclavicular area, and internal mammary chain. 3D surface volume rendering SPECT/CT ( ) identifies the anatomical correlates of the SLNs localized on transaxial SPECT/CT and CT slices to the second intercostal space and level I of the left axilla, respectively ( , ), as well as behind the left clavicle ( , ), as indicated by dashed yellow circles.",yes
PMC8602207,Figure_7,oa_package/dc/30/PMC8602207.tar.gz,[],"Figure7 The differences in immunocyte infiltration degree and enrichment plots of immune-related gene sets from Gene Set Enrichment Analysis (GSEA) between the high- and low-risk TCGA cohorts. The correlation with immunocyte infiltration was performed by using Pearson correlation analysis. M2; Tregs; NK cells; CD4+T cells. , Correlation with immune-checkpoint expression. PD-1; CTLA4. , GSEA analysis revealing immune-related biological processes correlated with the signature. ***P < 0.001.",yes
PMC2615016,Figure_3,oa_package/5f/ff/PMC2615016.tar.gz,['Electron micrographs demonstrating internalization of A.'],Figure 3 . NCI-H292 cells were infected with 05KA103 at an MOI of 100 for 5 h. (A and B) SEM images showing interaction of with NCI-H292 cells. The cell membrane was extended to and wrapped around (arrow). Bar represents 2 m. (C to E) TEM images. (C) loosely anchored to the cell surface (arrow) and small protrusion from the cell was appeared (arrow head). (D) The cell membrane was fused after the bacterial internalization (arrow). (E) Intracellular bacteria were surrounded by a membrane-bound vacuole (arrow head). Bar represents 1 m.,yes
PMC6102422,Figure_12,oa_package/8f/64/PMC6102422.tar.gz,[],"Fig. 12 (A): Midsagittal computed tomography myelography in a 5-year-old female pug with T10-T11 dorsal arachnoid diverticulum (arrow). (B): Left parasagittal multiplanar reformatted bssfp MRI image in a 10-year-old male pug with left dorsolateral T12-T13 arachnoid diverticulum (arrow). MRI provides optimal morphologic details of the arachnoid layer, without the need of subarachnoid contrast medium administration. Moreover, MRI images permit the assessment of the presence and extension of compressive myelopathy, seen as intramedullary hyperintensity on bright-fluid sequences (B, asterisk), which is of important prognostic value. CTM does not provide information on parenchymal attenuation changes secondary to spinal cord compression.",yes
PMC9694487,Figure_1,oa_package/31/78/PMC9694487.tar.gz,"['Given that this has already been extensively reviewed in the literature [3,9,10,11], this section discusses updates on potential targets and provides a framework to investigate the potential targets for disease-modifying therapy (, Table A1).', '1177/1759720X20969261The effect of T cells on the pathologics of pSS.']",Figure 1 The effect of T cells on the pathologics of pSS.,yes
PMC7799199,Figure_2,oa_package/37/2e/PMC7799199.tar.gz,"['Initial electrocardiogram sinus rhythm, heart rate: 116 b.']","Figure 2 Initial electrocardiogramsinus rhythm, heart rate: 116 b.p.m., normal QRS axis, PQ: 200ms, QRS: 80ms, discrete ST depression across the inferior leads, J-point elevation across the anterior leads, 1-1 isolated ventricular extrasystoles.",yes
PMC4834154,Figure_1,oa_package/45/99/PMC4834154.tar.gz,['0-6524917887619092109Initial (a) MRI with unremarkable diffusion-weighted imaging (DWI) and perfusion-weighted imaging (PWI) as well as FLAIR images.'],Figure 1 Initial (a) MRI with unremarkable diffusion-weighted imaging (DWI) and perfusion-weighted imaging (PWI) as well as FLAIR images. Follow-up MRI (b) shows small ischemic lesions (arrow) in the left MCA territory on DWI. PWI is unremarkable. FLAIR images demonstrate gadolinium enhancement in the subarachnoid space in the left MCA territory (arrows).,yes
PMC9501595,Figure_3,oa_package/6a/49/PMC9501595.tar.gz,"['At 14 dpi, the main histological findings included locally extensive neuronal necrosis and loss in the pyramidal layers of CA1 and CA2 (A E).', 'Microglial/macrophage reactivity was more pronounced than at 4 dpi (F J), and neuroparenchymal and perivascular microglia/macrophages were observed.', 'Relative to 4 dpi, TMEV mRNA expression was more limited to the medial area of field CA1 and sparsely distributed throughout field CA2 (K O).', 'Cross sections of the hippocampal formation at level B of CC mice infected with Theiler s murine encephalomyelitis virus (TMEV) and euthanized at 14 dpi.']","Figure 3 Cross sections of the hippocampal formation at level B of CC mice infected with Theilers murine encephalomyelitis virus (TMEV) and euthanized at 14 dpi. ( ) (Hematoxylin and eosin stain) Locally extensive areas of neuronal necrosis and loss were observed in CA1 and CA2 pyramidal layers with granular mineralization of the stratum oriens above CA1. The stratum radiatum and stratum lacunosum moleculare exhibited moderate to marked gliosis and perivascular cuffing. ( ) (Iba-1 immunolabeling) Increased numbers of Iba-1 positive microglia/macrophages were widely distributed throughout the hippocampal formation. In the CC023 strain, the gliosis encompassed the dentate gyrus. ( ) (TMEV RNA in situ hybridization) TMEV mRNA expression was confined to the medial area of fields CA1 and CA2. In the CC023 strain, TMEV mRNA expression was observed in the dentate gyrus and underlying thalamus. Bar = 200 m.",yes
PMC6806458,Figure_1,oa_package/05/ce/PMC6806458.tar.gz,"['She had also undergone an MRI/MRCP at the outside hospital that revealed multiple cystic loculations of the pancreas that appeared to be possibly in communication with the main pancreatic duct ().', 'MRCP showing innumerable saccular dilations (A) associated with dilation of the main pancreatic duct and a 1.', '']",Fig. 1 MRCP showing innumerable saccular dilations (A) associated with dilation of the main pancreatic duct and a 1.2cm stone in the pancreatic head (B).,yes
PMC7431620,Figure_6,oa_package/1e/b1/PMC7431620.tar.gz,"['qRT-PCR analysis revealed a higher IFN mRNA expression level in the CP of APP / or APP+/+ mESC-TEP-transplanted AD mice, compared with control cell-treated mice (A).', 'Flow cytometric examination confirmed a significantly higher percentage of IFN -producing CD4+ immune cells in this compartment in APP / or APP+/+ mESC-TEP-transplanted AD mice (B).', 'Transplantation of APP / or APP+/+ mESC-TEPs increases IFN availability and leukocyte trafficking molecule expression in the CP.', 'As shown in C, the mRNA expression levels of these leukocyte trafficking molecules in the CP of APP+/+ mESC-TEP-treated AD mice were higher than those in control cell-treated mice.', 'The mRNA expression levels of these molecules (except vcam1) in the CP of APP / mESC-TEP-treated AD mice were higher than those in APP+/+ mESC-TEP-treated mice (C).']","Figure 6 Transplantation of APP or APP mESC-TEPs increases IFN availability and leukocyte trafficking molecule expression in the CP. 3XTg-AD mice were injected i.t. with APP mESC-TEPs, APP mESC-TEPs, or control cells as in . Two and half months later, the CP was harvested. mRNA levels of IFN were analyzed by qRT-PCR. Flow cytometric analysis for the percentage of IFN-producing cells in CD4 cells, (left) representative flow cytometric profiles, and (right) statistical analyses. mRNA levels of icam1, ccl2, vcam1, cxcl10 were analyzed by qRT-PCR. The expression levels of the genes in control cell-treated mice are defined as 1. The data are expressed as mean SD from one of three independent experiments with similar results (48 mice per group in each experiment). * < 0.05 vs. control cell group, ** < 0.05 vs. APP mESC-TEP group.",yes
PMC5652621,Figure_4,oa_package/e1/71/PMC5652621.tar.gz,"['Immunohistochemical studies revealed that the dendritic cells in the germinal centres were positive for HIV-1 p24 antigen, suggesting that they were HIV-infected (figure 4).', 'Immunohistochemical test results revealed HIV-1 p24 antigen in the follicular centre, and the dendritic cells in the follicular centres were positive for HIV-1 p24 antigen (original magnification was 100 (A) and 400 (B)).']","Figure 4 Immunohistochemical test results revealed HIV-1 p24 antigen in the follicular centre, and the dendritic cells in the follicular centres were positive for HIV-1 p24 antigen (original magnification was100 (A) and400 (B)).",yes
PMC6178320,Figure_5,oa_package/5d/45/PMC6178320.tar.gz,"[' illustrates an example of prostate cancer with fused glands graded as GP 4.', 'Prostate cancer Gleason pattern 4 with fused glands.']",Figure 5 Prostate cancer Gleason pattern 4 with fused glands. Note group of glands that are no longer completely separated by stroma. Bar 50 m; H&E stain.,yes
PMC9358607,Figure_7,oa_package/2a/af/PMC9358607.tar.gz,"['The shapes of the SF were classified as oval, round, and heart (A-C).', '\nThe shapes of Stenson foramen in axial section.']",Figure 7 The shapes of Stenson foramen in axial section. A = round; B = oval; C = heart.,yes
PMC9016092,Figure_4,oa_package/59/2e/PMC9016092.tar.gz,[],"FIGURE 4 Experimental approaches for dissecting the activity of regulatory lncRNAs. (a) ASO gapmers act co or posttranscriptionally to trigger RNAse Hdependent degradation of target RNAs (Lai et al., ; Lee & Mendell, ); (b,c) Insertion of short genetic elements such as a PAS or selfcleaving ribozyme permits the stable, specific knockdown of host lncRNAs (Engreitz et al., ; Latos et al., ; Sleutels et al., ; Tuck et al., ); (d,e) CRISPRi, based on recruitment of catalytically inactive dead Cas9 (dCas9), may disrupt transcription by sterically blocking transcriptional elongation by Pol II (Dahlman et al., ). This inhibitory effect can be augmented by fusing Cas9 to repressive chromatinmodifying proteins such as the KRAB domain (Gilbert et al., ). Analogously, CRISPRa, exploits the ability of dCas9 to target fused activating domains to promoters, thereby boosting transcription (Gilbert et al., ); (f) Various genetic approaches, such as sequence inversion (Mohammad et al., ), 5 splice site (SS) or downstream exon deletions (Allou et al., ; Engreitz et al., ) have been used to investigate the importance of specific sequence elements. Table indicates whether the approach is expected to affect DNA elements (DNA), act of transcription (TXN), or the RNA molecule (RNA); Yes, the element is affected, No, the element is not affected, and ? the effects are unknown or contextspecific",yes
PMC5478206,Figure_1,oa_package/ab/69/PMC5478206.tar.gz,"['We now analyzed lung tissue of male and female heterozygous Slc40a1wt/C326S and homozygous Slc40a1C326S/C326S mice and demonstrate that iron accumulates in an age- and genotype-dependent manner (A).', 'Slc40a1C326S mice show increased pulmonary iron content.', 'Under physiological conditions, elevated cellular iron levels inactivate the iron-responsive element/iron-regulatory protein (IRE/IRP) system.', 'Accordingly, mRNA and protein expression of TfR1 and mRNA expression of the IRE-containing transcript of DMT1 are decreased in iron loaded lungs of Slc40a1wt/C326S and Slc40a1C326S/C326S mice (B, C, D), while FtL protein expression is increased (', 'Furthermore, FPN expression shows a genotype-specific increase both at mRNA and protein levels (D, E).', 'Consistently, increased pulmonary iron levels in Slc40a1C326S/C326S mice correlate with increased heme oxygenase 1 (HO-1) mRNA expression, a marker for oxidative stress (F), and with elevated lipid peroxidation (']","Fig. 1 Slc40a1 mice show increased pulmonary iron content. A) Total lung non-heme iron levels measured in male and female 10-, 24- and 36-week old mice. B) qRT-PCR analysis of TfR1 mRNA expression in total lung of female 36-week old mice. C) qRT-PCR analysis of DMT1-IRE mRNA expression in total lung of female 36-week old mice. D) Western Blot analysis of TfR1, FtL and FPN protein expression in total lung of female 36-week old mice. -actin was used as loading control. E) qRT-PCR analysis of FPN mRNA expression in total lung of female 36-week old mice. F) qRT-PCR analysis of HO1 mRNA expression in total lung of female 36-week old mice. G) Lipid peroxidation measured by the TBARS assay in total lung of female 36-week old mice.",yes
PMC2838913,Figure_2,oa_package/2c/72/PMC2838913.tar.gz,"['Saggital and coronal T2/STIR-weighted magnetic resonance scan of the foot demonstrating calcaneal edema and edema within the tarsal tunnel, with a tense adjacent subtalar joint effusion.']",Figure 2 .,yes
PMC7841211,Figure_1,oa_package/70/ee/PMC7841211.tar.gz,"['Abdominal ultrasound and computed tomography (CT) scan revealed a perforated appendix containing two dense structures that were considered appendicoliths and two small abscesses ().', 'CT scan showing perforated appendix containing two appendicoliths.', 'The procedure was performed by a senior resident with five years of experience.']",Fig. 1 CT scan showing perforated appendix containing two appendicoliths.,yes
PMC6513907,Figure_5,oa_package/8c/0a/PMC6513907.tar.gz,"[' 5a and b, a patient with severe left-sided idiopathic EH and a clinical diagnosis of left MD, the left ES terminated prematurely in the operculum, and the entire eES portion was missing (', ' 5a; arrow marks the distal end of the ES in the operculum).', ' 5b).', ' 5c, open circles indicate specimens with ES hypoplasia; other data points as shown in ', ' 5a, b, with left ES hypoplasia) or bilateral pathology and was always associated with an ipsilateral MD diagnosis and ipsilateral EH.', '', 'Scale bars: a, b 50 mLoss or absence of ALDO-regulated proteins in degenerative and hypoplastic ES pathologyTo investigate expression patterns of ALDO-regulated proteins in ESs affected by degenerative or hypoplastic pathology, we immunostained ES epithelia from selected human cases.']","Fig.5 ES hypoplasia in idiopathic EH cases without degenerative ES pathology. , ES morphology in the right ( ) and left inner ear ( ) in a patient with left severe idiopathic EH and a diagnosis of left definite MD; note the hypoplastic left ES, with the distal end of the ES in the operculum (arrow in ). Among the 14 specimens with idiopathic EH that did not show eES degeneration (data points in box), 11 specimens (78.6%) demonstrated ES hypoplasia (open circles). Same data as presented in Fig. a. Scale bars: , 50m",yes
PMC5519492,Figure_2,oa_package/b1/49/PMC5519492.tar.gz,"[', 2010) ( 2A).', ' 2Inducible-Conditional Depletion of TDP-43 from Microglia Induces Enhanced Phagocytosis of A 42 Oligomers Administered by Stereotactic Injections(A) Schematic representation of mouse breeding strategy for microglia-specific inducible conditional line, to obtain Cx3cr1CreER;Tardbp+/+ (WT) and Cx3cr1CreER;Tardbpfloxed/floxed (cKO) experimental subjects.', ', 2010) and quantifying amyloid uptake 24 hr later (s 2B 2E).', 'Interestingly, we observed a significant increase of microglia cells in close proximity to the amyloid core in cKO mice compared to WT littermates, whereas no differences occurred in the area surrounding the A core (s 2D and 2F).']","Figure2 Inducible-Conditional Depletion of TDP-43 from Microglia Induces Enhanced Phagocytosis of A42 Oligomers Administered by Stereotactic Injections (A) Schematic representation of mouse breeding strategy for microglia-specific inducible conditional line, to obtain Cx3cr1 ;Tardbp (WT) and Cx3cr1 ;Tardbp (cKO) experimental subjects. (B) Timeline for stereotactic injections of A oligomers upon tamoxifen treatment in WT and cKO mice and relative coordinates of injections. (C and D) 3D reconstruction of confocal stack acquisition in the somatosensory cortex of WT and cKO mice, 24hr after the injection of 100M A42 oligomers (C).Dashed-yellow frames enclosing A core are zoomed in (D), showing a representative reconstruction of Iba1-positive microglia processes in green, surrounding (WT) or infiltrating (cKO) the 6E10-positive amyloid core in red. Increased engulfment of amyloid is appreciable in cKO microglia cells compared to WT controls. (E and F) Quantification of microglia processes and A engulfment surrounding or within amyloid core injection. Data are shown as mean SEM from WT, n= 8, and cKO, n= 7, stacks, acquired from n= 3 animals per genotype, p< 0.05, p< 0.01, using two-way ANOVA followed by uncorrected Fishers LSD test.",yes
PMC9731827,Figure_6,oa_package/08/2e/PMC9731827.tar.gz,"['Fluoroscopy confirms additional Tornado and Nester coil placement in the dominant inflow vessel and the second inflow vessel.', 'Angiography demonstrating no filling of AVM through the second inflow vessel after embolization with Nester coil.']",Fig. 6 Fluoroscopy confirms additional Tornado and Nester coil placement in the dominant inflow vessel and the second inflow vessel.,yes
PMC7585435,Figure_5,oa_package/28/14/PMC7585435.tar.gz,"['5A, B).', '5C) and vimentin (', '5D).', '5A) and did not co-localize with aggresomal markers (', '5C, D), suggesting that MRPs and RBPs sequester TDP-43 into their aggregates through distinct mechanisms.', 'MRPs-induced TDP-43 aggregates are dependent on microtubules and co-localized with aggresome markers.', 'Histone deacetylase 6 is associated with the co-aggregation of MRPs and TDP-43Histone deacetylase 6 (HDAC6) recognizes misfolded proteins and transports them to aggresomes through microtubules28.']","Fig. 5 MRPs-induced TDP-43 aggregates are dependent on microtubules and co-localized with aggresome markers. Nocodazole, a microtubule destabilizing reagent, dissociated TDP-43 co-aggregates with the MRP (PFN1) but not with the RBP (FUS). Arrowheads indicate co-aggregates of the MRP/RBP and TDP-43 -EGFP ( ). The ratio of the cells with TDP-43 aggregates was quantified by counting more than 100 cells in each trial ( ). Data are expressed as meanSEM ( =3). , Representative immunofluorescence images showed that the MRPs (PFN1 and TUBA4A) induced TDP-43 co-aggregates were co-localized with the aggresomal markers -tubulin ( ) and vimentin ( ). Arrowheads indicate the co-localization of MRPs, TDP-43, and -tubulin in or vimentin in . Note that there were no aggregates in RBPs, represented by FUS, because the aggregates of RBPs were not observed in this immunocytochemical condition. Scale bars=20m.",yes
PMC6327279,Figure_3,oa_package/36/1c/PMC6327279.tar.gz,"['Therefore, the preprocessing stage is necessary to obtain the binary image of the intimal layer (the most inner layer of three layers building the vessel wall) without artifacts from the diagnostic catheter and improve the quality of the image for further analysis ().', '002""/>Output of the preprocessing step: (a) OCT input image, (b) artifact removal, (c) OCT image after polar transformation, and (d) primary segmentation of the lesion in polar coordinates.']","Figure 3 Output of the preprocessing step: (a) OCT input image, (b) artifact removal, (c) OCT image after polar transformation, and (d) primary segmentation of the lesion in polar coordinates.",yes
PMC3691657,Figure_3,oa_package/e0/14/PMC3691657.tar.gz,['Antibody based identification of CYB5A as S-nitrosylated protein.'],"Figure 3 2DE Spot representative differential expression level of CYB5A as S-nitrosylated protein in HCC compared with respective fibrotic liver and HepG2 cell line. The proteins were immuno-precipitated with anti SNO-Cys antibody, focused on IPG strips (pI 3-11NL; 7 cm) and separated on 12.5% gel followed by silver staining. Representative spots of CYB5A immuno-precipitated with anti SNO-Cys antibody. 100 g proteins were focused using pI 3-11NL (7 cm) IPG strips and separated on 12.5% gel followed by western blotting described in material and methods. Densitrometric analysis was performed using Progenesis SameSpots v4.5 (Nonlinear Dynamic, UK).",yes
PMC8645473,Figure_3,oa_package/ec/4f/PMC8645473.tar.gz,"['The lesion showed significant ring enhancement with the passage of the contrast medium [].', ':Brain magnetic resonance imaging in sagittal slices where intra-axial image is observed in the left precuneus.']",Figure 3: Brain magnetic resonance imaging in sagittal slices where intra-axial image is observed in the left precuneus. T1 with gadolinium (a) and T2 (b).,yes
PMC10206376,Figure_1,oa_package/0d/56/PMC10206376.tar.gz,"['Contrast extravasation had pooled into a loculated area superior and anterior to the left ventricle, stemming from the anterior margin of the interventricular septum (, ).', 'Sagittal Chest CT emphasizing the penetrating trajectory of the knife, coursing posteriorly above the left fifth rib and through the anterior wall of the left ventricle (LV).', 'Axial MIP Chest CT identifying the communicating defect of the contained pseudoaneurysm (PSA) involving the right ventricle (RV) and left ventricle (LV).']","Fig. 1 Sagittal Chest CT emphasizing the penetrating trajectory of the knife, coursing posteriorly above the left fifth rib and through the anterior wall of the left ventricle (LV). The penetrating wound traveling from the anterior chest wall to the left ventricle measured 10 cm in length. The large ventricular pseudoaneurysm (PSA) communicates with the left ventricle (LV).",yes
PMC6142361,Figure_2,oa_package/5b/6e/PMC6142361.tar.gz,"[' 2a), and if patients needed an IVR, the table could be rotated (', '2c).', 'a shows that C-arm and CT gantry are located at the opposite side.', 'd shows intervention mode in which surgeries and interventional radiology are performed after rotating the patient tableBasically, severe trauma patients are directly transported to this hybrid ER system by the emergency medical services.', '2b).', '2d).']",Fig. 2 shows that C-arm and CT gantry are located at the opposite side. shows CT mode in which primary survey and CT are performed. shows rotating patient table. shows intervention mode in which surgeries and interventional radiology are performed after rotating the patient table,yes
PMC3512286,Figure_10,oa_package/ac/eb/PMC3512286.tar.gz,[],Figure 10 Testicular seminoma: the relationship between the tumour and the tunica albuginea is well evident.,yes
PMC6538732,Figure_1,oa_package/cf/37/PMC6538732.tar.gz,"[' 1.', 'Example images from two subjects with complete PCL tears.', '0 mm; FOV: 125 mm; TA: 3 min 19 s)The remaining 6 subjects, who had continuous-appearing PCLs, were analyzed.']",Fig. 1 Example images from two subjects with complete PCL tears. Subject A had a chronic injury in which the PCL was detached from the femoral attachment (white arrow). Subject B had a chronic injury in which the PCL was partially torn at its tibial attachment (black arrow). Images shown are from three consecutive slices of i) a PD FS TSE sequence (sagittal plane; TR/TE: 5300/40ms; interpolated resolution: 0.190.19mm; slice thickness: 3.0mm; FOV: 120mm; TA: 2min 50s) and ii) a PD TSE sequence (sagittal plane; TR/TE: 2500/32ms; interpolated resolution: 0.160.16mm; slice thickness: 3.0mm; FOV: 125mm; TA: 3min 19s),yes
PMC11315307,Figure_3,oa_package/40/dc/PMC11315307.tar.gz,"['001) (A).', 'Additionally, tolerance to methylglyoxal and H2O2, which are substrates and reaction products of the enzyme, respectively, were significantly decreased for the MaGlox mutant (B and 3C).', 'In contrast, the MaGlox-OE strain demonstrated significantly increased tolerance to H2O2 compared to the wild type (B and 3C).', 'Staining for cellular ROS levels with the peroxide indicator dihydroethidium (DHE) revealed strong fluorescence in the MaGlox strain, whereas only a weak signal was observed in the hyphae of the wild type and MaGlox-OE strains for cells grown for 20 h, and fluorescence was strongly increased at the 36 h growth time point for all three strains (D).', '01, E and 3F).', '01, G).', 'Furthermore, intracellular H2O2 levels increased 10 12 fold in the MaGlox mutant as compared to the wild type strain (H).', 'g003MaGlox is essential for maintaining intracellular balance of redox.', 'Expression of autophagy-related (atg1, atg3, atg4, atg8, atg11, atg12, atg13, atg17) and apoptosis-related genes (Cas, CasA1, CasA2) were also significantly decreased when CAR and MESNA were added during growth of the MaGlox mutant (S A and 3B).']",10.1371/journal.ppat.1012431.g003,yes
PMC2972610,Figure_6,oa_package/4e/f0/PMC2972610.tar.gz,"['Also, in another two patients referred with polytrauma, R II data, which were found to be more statistically significant, were scored with 3 points higher on average than R I in the aspects of orientation, short processing and adaptation time (5 mins versus 11 mins) for acquiring critical information (, ).', 'Cranial trauma.']","Figure 6 Cranial trauma. ) Cerebral herniation from the defect in the left parietal area. b) All drainage tubes, catheters and the defect area can be seen in different 3D Windows ( , ). In the same patient it was also detected the abdominal catheters (drainage tubes, gastrostomy tube, ) and buried calvarium in the abdominal wall ( ).",yes
PMC6783181,Figure_3,oa_package/cb/64/PMC6783181.tar.gz,"['Musculoskeletal ultrasound (MSUS) findings 1 year after starting ADA, in November 2018.']","Figure 3 Musculoskeletal ultrasound (MSUS) findings 1 year after starting ADA, in November 2018. There was no active synovitis in the (A) right and (B) left first metatarsophalangeal (MTP) joint and (C) right knee joint. (D) There was no active tenosynovitis in the right bicep tendon.",yes
PMC6907722,Figure_5,oa_package/d5/93/PMC6907722.tar.gz,['Bladder TCC (MRI)(A-D) Selected axial MR urography images of the same patient demonstrate a lobulated mass (arrow) arising from the right lateral wall of the urinary bladder which extends to the trigone and invades into the urinary bladder wall to involve the outer layer without extension into the perivesical fat.'],"Figure 5 Bladder TCC (MRI) (A-D) Selected axial MR urography images of the same patient demonstratea lobulated mass (arrow) arising from the right lateral wall of the urinary bladder which extends to the trigone and invades into the urinary bladder wall to involve the outer layer without extension into the perivesical fat. The mass is of T1 low and T2 high signal intensity. The mass demonstrates relatively homogeneous, diffuse contrast enhancement and prominent diffusion restriction (b50/400/800).The constellation of findings reflects a bladder tumour which is probably staged as T2b No Mx. TCC:Transitional cell carcinoma",yes
PMC9452647,Figure_1,oa_package/4b/06/PMC9452647.tar.gz,[],Figure1 Preoperative echocardiography demonstrated a large lobulated mass (arrows) attached by a pedicle to the posterior wall of pulmonary truck. Transverse CT angiogram revealed a diffused occlusion of both pulmonary arteries (arrows).,yes
PMC7606448,Figure_4,oa_package/23/12/PMC7606448.tar.gz,"[' 4 describes the survival analysis results for each cohort using the top two traits by test performance from Table 1 in each (MKI67 and miR-17 for breast and miR-17 and KRAS for lung).', ' 4a).', ' 4c).', 'Survival analysis with respect to HTI derived from two traits in breast and lung cancer.', 'DiscussionThis work offers a method for analyzing tumor heterogeneity from the rich spatial data available in H E WSIs.']","Figure 4 Survival analysis with respect to HTI derived from two traits in breast and lung cancer. For each trait combination in ( ) and ( ), slides were split into high and low HTI ( and or blue and orange respectively). In ( ) slides were split into and HTI. Each survival curve is shown with a 95% confidence interval.",yes
PMC10634831,Figure_3,oa_package/ac/92/PMC10634831.tar.gz,"['Using whole-mount immunofluorescence, we confirmed that ENS macrophages express C1q protein and that expression is increased in PFF-injected mice (A,B).', 'Interestingly, we observed C1q+/pSer129 -syn+ punctae within ENS macrophages, and these were also increased in PFF-injected mice (C) indicative of complement-mediated internalization of -syn by ENS macrophages.', 'These findings were associated with increased deposition of C1q on myenteric neurons (D,E), which, taken together, suggest that myenteric macrophages utilize complement to engulf syn from enteric neurons.', 'To validate that complement dependent engulfment -syn was a protective mechanism against enteric synucleinopathy, we repeated our gut PFF injections in C1qa / mice and WT controls (F).', 'Loss of C1q resulted in a further increase in PFF-induced enteric neuronal pSer129 -syn+ staining compared to WT controls (G, H).', 'The exacerbated neuronal pathology was accompanied with a decrease in the number of internalized pSer129 -syn+ punctae within macrophages (I, J), supporting interpretation that C1qa / mice were less efficient at clearing -syn from neurons, leading to spread of pSer129+ pathology.', 'Indeed, we found decreased fecal output in our PFF-injected mice relative to saline controls that was partially rescued by loss of C1q (L).', '563832v1-f0002"" position=""float""/>:ENS-resident macrophages engulf -syn from neurons via a C1q-dependent mechanism.']","Figure 3: ENS-resident macrophages engulf -syn from neurons via a C1q-dependent mechanism. (A) Representative confocal images of myenteric macrophages (MHCII, blue) at 30dpi saline or PFFs showing expression of C1q (magenta) and co-localized punctae of C1q with pSer129 -syn (magenta, white arrowheads) in PFF mice. (B) Quantification of C1q+ area and intensity within myenteric macrophages showing upregulation in PFF-injected mice Mann-Whitney test, p<0.05, n=35 animals/group). (C) Quantification of the number of C1q+/pSer129 -syn+ punctae within myenteric macrophages showing an increase in PFF mice (unpaired t-test, p<0.01, n=35 animals/group). (D) Representative confocal images of myenteric ganglia (peripherin, cyan) at 30dpi with either saline or PFF showing pSer129 -syn+ soma (yellow), and deposition of C1q+ punctae (magenta) on neuronal soma. (E) Quantification of the C1q+ area (left) and intensity (right) on myenteric neurons in saline and PFF mice showing an increase with PFF injection (unpaired t-test, p<0.05, n=34 animals/group). (F) Cartoon depicting experimental design for C1qa experiments. (G) Quantification of the number of pSer129 -syn+ neuronal soma expressed as a percentage of total peripherin+ soma within the myenteric plexus of saline- and PFF-injected mice at 30dpi in both C1q and WT controls showing a PFF-induced increase in pathology that was further enhanced in the C1q mice (two-way ANOVA p <0.0001, p <0.01, p <0.05. Tukeys test for multiple comparisons WT-saline vs WT-PFF p<0.05, WT-saline vs C1q -saline p=0.9987, C1q -saline vs C1q -PFF p<0.0001, WT-PFF vs C1q -PFF p<0.001). (H) Representative confocal images of myenteric ganglia (peripherin) showing qualitatively increased pSer129 -syn+ (color) neurons in C1q -PFF animals. (I) Representative confocal images of myenteric macrophages (MHCII, color) with internalized pSer129 -syn+ punctae (color, white arrowhead) in PFF injected C1q mice and WT controls 30dpi. (J) Quantifcation of the number of internalized pSer129 -syn+ within MHCII+ myenteric macrophages showing decreased punctae in C1q macrophages (unpaired t-test, p<0.01). (K) Linear regression analysis of 139 myenteric ganglia comparing the percentage of pSer129+ neurons against the neuron-macrophage contact showing a statistically significant positive correlation that did not differ between genotypes (p=0.5609, left). Average contact size between macrophages and myenteric ganglia in PFF-injected WT and C1q mice revealing no significant difference between groups (unpaired t-test, right). (L) Fecal output measured by the number of pellets or the total weight of pellets after a 30-minute period showing a decrease in the PFF group that was partially rescued by loss of C1q. Pellet count (left): two-way ANOVA p <0.001, p <0.05 with Tukeys test for multiple comparisons WT-saline vs WT-PFF p<0.01, WT-saline vs C1q -saline p=0.9819, C1q -saline vs C1q -PFF p=0.100, WT-PFF vs C1q -PFF p<0.05. Pellet weight (right): two-way ANOVA p <0.0001, p <0.001 with Sidaks test for multiple comparisons WT-saline vs WT-PFF p<0.01, WT-saline vs C1q -saline p=0.2319, C1q -saline vs C1q -PFF p<0.05, WT-PFF vs C1q -PFF p<0.01.",yes
PMC8499537,Figure_9,oa_package/b0/72/PMC8499537.tar.gz,"[' 9a c).', ' 9b and c, in addition to the alteration of microtubules (green signal), the treatment with ZnO NPs caused the degradation of F-actin structures (red signal), when compared to untreated cells (', 'c Cells treated with 20 g/cm2 ZnO NPsIn particular, the incubation with 5 g/cm2 ZnO NPs for 24 h (', ' 9a c).', ' 9b).', ' 9c).']",Fig. 9 Effects of ZnO NPs treatment for 48h on actin cytoskeleton and morphology of A549 cells. Confocal microscopy analysis and scanning electron microscopy observations. Control cells. Cells treated with 5g/cm ZnONPs. Cells treated with 20g/cm ZnO NPs,yes
PMC7993245,Figure_21,oa_package/e0/5d/PMC7993245.tar.gz,[],Figure 5e: Examples of examination-level annotations on axial CT images. Ground-glass opacities surrounding a nodular opacity (arrow) in the left lower lobe (halo sign). Bilateral ground-glass opacities (arrows) with central clearing (reversed halo sign). Reticular pattern without parenchymal opacity in the left upper lobe (arrows). Perilesional vessel enlargement associated with bilateral ground-glass opacities (arrows). Bronchial wall thickening most evident in the right lung (arrows). Bronchiectasis in the left upper lobe (arrows). Bilateral subpleural curvilinear lines (arrows). Small bilateral pleural effusions (arrows). Right pleural thickening (arrows). Right pneumothorax (arrows). Pericardial effusion (arrow). Mediastinal lymphadenopathy (arrows) in the prevascular and bilateral lower paratracheal stations. Pulmonary emboli (arrows) in the right lower and middle lobar pulmonary arteries.,yes
PMC5608709,Figure_6,oa_package/1d/86/PMC5608709.tar.gz,"[' 6A).', ' 6B).', ' 6C).', ' 6D).', ' 6E), and the adherent ratio was remarkable higher at 8 h.', ' 6F).', ' 6G, AIIB2 treated mice had significantly lower fecal counts of C.', 'S100A4 increases C.', '\nDiscussionIn this study, we demonstrated that S100A4 promotes colitis development via increasing the adherence of C.']","Figure 6 S100A4 increases adherence to CT26 cells. ( ) FISH analysis of conventional WT and mice colon on day 0 and day 7. Colon tissues were probed with a universal bacterial FISH probe (green) and counterstained with DAPI (blue), (scale bars, 20m). Representative images of the distal colon were shown ( =3). ( ) Real-time quantitative PCR analysis of the expression of mRNA encoding adhesion molecules in WT and mice colons on day 7 p.i. GAPDH was used as the reference control, ( =4); ** <0.01. The mRNA level of WT mice is set as 1.00 to calibrate the relative level of mice. ( ) Real-time quantitative PCR analysis of the expression of mRNA encoding adhesion molecules in CT26 cells treated for 0 or 8h with S100A4. GAPDH was used as the reference control, ( =4); ** <0.01. The mRNA level of 0h is set as 1.00 to calibrate the relative level of 8h. ( ) Real-time quantitative PCR analysis of the expression of mRNA encoding adhesion molecules in CT26 cells treated for 0 or 8hours with S100A4 after stimulating with FPS-ZM1 (10g/ml) for 1h. GAPDH was used as the reference control, ( =4); * <0.05. The mRNA level of cells that are not administrated with inhibitor FPS-ZM1 is set as 1.00 to calibrate the relative level of cells administrated with inhibitor FPS-ZM1. ( ) The adherent ratios of to a CT26 cell monolayer were quantified by calculating CFU. CT26 cells were pretreated with S100A4 (1000 ng/ml) for 08h before infection with , ( =4); * <0.05. ( ) The adherent ratio of to CT26 cells was quantified after stimulating with integrin blocker AIIB2 (2.5g/ml) for 1h and S100A4 for 8h, ( =4); ** <0.01. ( ) Bacterial titers in fecal homogenates from AIIB2 treated WT mice and control WT mice on day 7 after . infection ( =5); * <0.05.",yes
PMC10455446,Figure_4,oa_package/81/33/PMC10455446.tar.gz,"['041, Tukey s test, BH-corrected) (C).', '0398, Tukey s test, BH-corrected) (C).', '029, Tukey s test, BH-corrected) (C).', '0256, Tukey s test, BH-corrected) (C).', 'Spine microglial density changes in siponimod-treated TMEV animals: Spine tissue sections were stained for Iba1 and DAPI to label microglial cells and all cell nuclei, respectively.']","Figure 4 Spine microglial density changes in siponimod-treated TMEV animals: Spine tissue sections were stained for Iba1 and DAPI to label microglial cells and all cell nuclei, respectively. Images ( ) are representative images of the spine white matter region, collected from healthy control, vehicle-treated, and siponimod-treated animals, respectively. ( ) are representative images of the spine gray matter region collected from healthy control, vehicle-treated, and siponimod-treated animals, respectively. ( , ) are graphs presenting the density of Iba1-labeled cells in white matter and gray matter, respectively. ( , ) are graphs presenting percent fraction of the Iba1-labeled cells presenting type 1, 2, and 3 morphology. The inset in ( ) contains representative images for normal morphology (type 1), activated branched morphology (type 2), and (type 3) amoeboid morphology. Green, blue, and yellow circles identify Iba1-labeled cells presenting type 1, 2, and 3 morphology, respectively, in ( ). * < 0.05, for BonferroniHochberg-corrected value for individual timepoint comparison with HC animal data. The red bar represents animals treated with siponimod at 3 mg/kg, the olive-green bar represents vehicle-treated, and the blue bar represents healthy controls. The color assignments for the different treatments are outlined in the figure. The scale bar refers to 25 m length. The distance between the two whiskers represents inter-quartile distance from first to third quartile.",yes
PMC4500065,Figure_2,oa_package/06/5d/PMC4500065.tar.gz,['21).'],Fig. 2. A cyst of in the brain (case No. 50),yes
PMC8414134,Figure_1,oa_package/be/57/PMC8414134.tar.gz,"['The predictive power of LAVi has been enhanced by the advent of three-dimensional echocardiography (3DE) (6, 7), which allows a more precise evaluation of the left atrial volume (LAV) without geometric assumptions and foreshortening (8) ().', 'Values from 3DE better correlate with the volume obtained with3D echo reconstruction of the left atrium (LA) and left ventricle (LV).']",Figure 1 3D echo reconstruction of the left atrium (LA) and left ventricle (LV). LA is shown at its end-diastolic phase in order to appreciate left maximal atrial volume.,yes
PMC10720202,Figure_1,oa_package/f5/b8/PMC10720202.tar.gz,"[' 1) can be applied to identify the presence and severity of left ventricular hypertrophy (LVH) that occurs in athletes [10].', 'Representation of 2D echocardiography: A B Parasternal long-axis view in diastole; C Apical 4-chamber view in diastole; D Apical 4-chamber view in systole.', 'From these views, detailed assessment of left ventricular dimensions, wall thickness, and volumes can be performedA study by Kreso et al.']","Fig. 1 Representation of 2D echocardiography: Parasternal long-axis view in diastole; Apical 4-chamber viewin diastole; Apical 4-chamber viewin systole. From these views, detailed assessment of left ventricular dimensions, wall thickness, and volumes can be performed",yes
PMC5957065,Figure_5,oa_package/bb/56/PMC5957065.tar.gz,"['H E stained corneal sections (A) were scored by a blinded veterinary pathologist utilizing a scoring matrix.', 'Each sample received a score as described in Table 1 (B).', 'The presence of epithelial mitotic figures in injured cornea sections indicated increased cellular proliferation and re-epithelialization and was not significantly different among injured groups (C).', 'Histological analysis of guinea pig eyelid skin.']","Figure 5 Histological analysis of guinea pig corneas. Representative images of hematoxylin and eosin stained cornea sections ( ). Comparison of key pathology observations between treatment groups ( ). Comparison of total number of epithelial mitotic figures per group ( ). No clinically relevant findings or any significant differences were observed. HPMC, hydroxypropyl methylcellulose.",yes
PMC9714778,Figure_1,oa_package/ca/10/PMC9714778.tar.gz,"['The second positive test occurred between 91 and 672 days after the initial test, with a median of 377 days and a peak approximately 1 year from the initial test (A).', 'Initial tests in patients who would later have a post 90-day positive test were primarily from March to May of 2020 and the winter of 2020/2021, whereas the post 90-day positive samples were largely collected in late 2021 and early 2022, when Omicron was the dominant variant (B).', '0005, Welch s t test) (, A and C).', 'While the number of samples that could not be sequenced in the group of post 90-day positive samples did increase along with increased testing toward the end of 2021, this was a small increase compared with the large increase in successfully sequenced isolates after 90 days starting in December of 2021 (D).', '3%) had sequence-validated reinfection based on identification of 2 high-quality genomes from different time periods that matched to different clades (E and Supplemental Table 1; supplemental material available online with this article; 29790939Version 109/01/2022In-Press PreviewVersion 210/24/2022Electronic publicationRepeat positive SARS-CoV-2 tests greater than 90 days apart.']","Figure 1 Repeat positive SARS-CoV-2 tests greater than 90 days apart. ( ) Kernel density estimator (KDE) plot showing the days from initial positive test. Color indicates whether sequencing was attempted or successful (gray, not attempted; blue, pass; red, fail) ( = 920 samples). ( ) KDE plot of date of initial positive and post90-daypositive tests ( of post90-day positive = 920). ( ) Number of tests that failed sequencing or provided high-quality genomes ( = 231). ( ) KDE plot showing sample collection dates of sequences that failed or provided high-quality genomes ( = 920). ( ) Bar plot showing persistence of initial genomes, inferred reinfection, or sequence-confirmed reinfection ( = 124 patients). ( ) Violin plot showing age and reinfection status in individuals with a post90-daypositive test. ( ) Bar plot showing days from initial positive test to post90-daypositive test. Color represents reinfection status ( = 127 samples). ( ) Bar plot showing sample collection date of post90-daypositive tests. Color represents reinfection status ( = 127 samples). ( ) Bar plot showing sex and reinfection status.",yes
PMC8622815,Figure_1,oa_package/ea/66/PMC8622815.tar.gz,"['In the canonical pathway (), the AHR functions as a ligand-activated transcription factor that directly regulates the expression of a wide range of target genes, named the AHR gene battery such as CYP1A, CYP1A2, and CYP1B1 enzymes of the CyP family [27,28,29,30] AHR repressor (AHRR) [31], and TCDD Inducible Poly (ADP-Ribose) Polymerase (TIPARP) [32,33].', 'In the absence of ligands, the AHR is confined in the cytosol that is associated with diverse chaperones, including a dimer of 90 kDa heat shock protein (HSP90), the co-chaperones p23, the AHR-interacting protein (AIP) (also known as ARA9 or X-Associated Protein-2 (XAP-2)), and the protein kinase Src () [41,42,43,44].', 'Upon agonist binding, the AHR changes its conformation, translocates to the nucleus, dissociates from its chaperone complex, and forms a heterodimer with a constitutively expressed nuclear factor knowns as an AHR nuclear translocator (ARNT) or as HIF-1 () [44,46,48].', 'LAT1 also mediates the L-Kyn efflux or influx in the blood barrier and immune cells, thus playing a major role in the regulation of AHR activation [84,85] ().', '1343729528494AHR genomic signaling pathway.']","Figure 1 AHR genomic signaling pathway. Before ligand binding, AHR is bound by a chaperone complex (described in the text and the figure), which maintains its localization in the cytoplasm. Cells are exposed to different AHR ligands, such as bioproducts of microbiota, phytochemicals, xenobiotics, or endogenous ligands, mostly derived from L-tryptophan (L-Trp). When a ligand binds, the AHR changes its conformation and c-Src and AHR-interacting proteins (AIP) are released, resulting in the exposure of the nuclear localization signal (NLS) in the AHRs N-terminus, that allows docking of importin and mediates nucleocytoplasmic shuttling. Once in the nucleus, the ligand-activated AHR heterodimerizes with its protein partner, the AHR nuclear translocator (ARNT), at the time it dissociates of cytoplasmic chaperone complex. ( ) The ligandAHRARNT heterodimeric complex binds specific DNA sequences located in the promoter regions of target genes, named xenobiotic responsive elements (XRE), and recruits additional coactivators and components of the transcriptional machinery (described in the text) that are required to initiate transcription of the target gene. ( ) The ligandAHRARNT complex can also interact with non-canonical AHR partners and regulate additional target genes. ( ) Canonical genes include enzymes of the cytochrome P450 (CyP) family and AHR repressor (AHRR). CyP enzymes metabolize AHR ligands and the AHRR competes with the AHR for interaction with the ARNT and DNA binding. After transcription, the AHR is exported out of the nucleus and is rapidly degraded by the proteasome. PAHpolycyclic aromatic hydrocarbon; HAHhalogenated aromatic hydrocarbon; ROSreactive oxygen species; HSP9090 kDa heat shock protein; EDCepidermal differentiation complex; TFtranscriptional factor. Figure was created with BioRender.com.",yes
PMC7692619,Figure_5,oa_package/17/1d/PMC7692619.tar.gz,"['As expected, we found activity in all tissues analyzed and the relative order of GLO1 activity was retina liver kidney brain heart RPE/choroid lens (A).', 'Regarding ocular tissues, we observed the highest activity of GLO1 in the retina of mice compared to the lens or RPE/choroid (B).', 'Glyoxalase activity is clearly tissue-dependent and retinal activity was about 9-fold and 13-fold greater than in the RPE/choroid and lens, respectively (C,D).', '5-fold greater than liver, heart, kidney, and brain, respectively (E H).', 'Evaluation of glyoxalase system activity in ocular and non-ocular tissues.']","Figure 5 Evaluation of glyoxalase system activity in ocular and non-ocular tissues. Glyoxalase I activity was determined spectrophotometrically using 1 mL quartz cuvettes by following the initial rate of formation of S-D-lactoylglutathione. The assay mixture containing the glycating reagent MG and reduced GSH was equilibrated at room temperature for 10 min, to ensure hemithioacetal formation. The reaction was initiated by the addition of 20 g of cytosolic extract and the A240 was monitored immediately and over the course of 5 min. The reaction rate was determined by following the increase in absorbance at 240 nm for which 240 = 2.86 mM cm . (A,B) Glyoxalase I activity was assayed in ( ) non-ocular tissues and ( ) ocular tissues and activity was expressed as milliunits per milligram of protein where one unit of GLO1 activity was the amount of enzyme which catalyzes the formation of 1 mol S-D-lactoylglutathione per min under assay conditions. ( ) Retinal glyoxalase I activity was compared to ( ) RPE/choroid, ( ) lens, ( ) liver, ( ) heart, ( ) kidney, and ( ) brain. Fold change was calculated relative to each tissue and values represent the mean standard error of the mean of 4 independent experiments from the GLO1 activity assay.",yes
PMC11509173,Figure_7,oa_package/9c/7f/PMC11509173.tar.gz,"['Thorough histopathological analyses highlighted the moderate formation of cellular material, consisting of spherical cells with pronounced nuclear polymorphism, multinucleated tumor cells, and an area of vascular proliferation ().', 'Microscopic image of histopathological analysis, showing spherical cells with pronounced nuclear polymorphism, multinucleated tumor cells, and an area of vascular proliferation.']","Figure 7 Microscopic image of histopathological analysis, showing spherical cells with pronounced nuclear polymorphism, multinucleated tumor cells, and an area of vascular proliferation.",yes
PMC11326701,Figure_2,oa_package/e8/54/PMC11326701.tar.gz,"['These cells fused with cells expressing ACE2 at 8 hpc, as indicated by the appearance of multinucleated cells (A).', 'Importantly, the fused cells exhibited enhanced SA- -gal and p21 staining, with increased transcription of the TNF, IL6, IL8 and CDKN1A genes (B), features that are typical of senescence.', 'The appearance of senescent syncytia began to occur at 12 h (C and 2D).', 'Eventually, approximately 70% of syncytia exhibited positive SA- -gal staining at 48 h upon S-EVs addition (C).', 'g002SARS-2-S delivery by EVs or mRNA triggers syncytial senescence.', 'Significant upregulation of senescence-associated genes supported the presence of a senescence phenotype in SWT -mRNA-LNP (F).', 'Consistent with previous studies, since the S sequence of the COVID19 mRNA vaccine contains two prolines mutations, which prevents cell fusion formation, and we observed that the SV-mRNA-LNP could not induce cell senescence (E).', 'RNA sequencing (RNA-seq) of liver samples revealed the upregulated expression of cellular senescence-related genes (H).', 'Finally, multiplex protein analysis of serum samples from these animals revealed that S-mRNA-LNP strongly induced SASP-reminiscent cytokines (I).']",10.1371/journal.ppat.1012291.g002,yes
PMC8679042,Figure_2,oa_package/d0/e7/PMC8679042.tar.gz,"['The patient returned approximately 6 weeks later with the similar exophytic nodule in the\ndorsal tongue, approximately 1 cm anteriorly to the previously noticed nodule ().', '.', '1177_2050313X211065884-fig2"" position=""float""/>.']",Figure 2. Recurrent exophytic nodule on dorsal tongue.,yes
PMC11308241,Figure_1,oa_package/1d/4c/PMC11308241.tar.gz,"['One case in this study, illustrated in 1, highlights the effectiveness of intraoperative TEE in manipulating catheters and for recognizing complications such as embolization.', ' 1Illustrative intraoperative transesophageal echocardiogram.']",Figure1 ( ) Panel A shows the right atrial mass attached to the junction of the inferior vena cava (IVC) and right atrium (RA). ( ) Panel B demonstrates the AngioVac catheter engaging the mass in the RA. ( ) Panel C shows the mass attached to the tip of the AngioVac catheter while withdrawing into the superior vena cava (SVC) during attempted extraction. ( ) Panel D shows the mass dislodging from the AngioVac catheter. ( ) Panel E demonstrates the mass mobilizing around the RA. ( ) Panel F shows the mass no longer present in the RA suggesting embolization.,yes
PMC4520273,Figure_3,oa_package/44/5e/PMC4520273.tar.gz,"[' 3).', 'Scale bar = 50 mTo further assess the involvement of LPS contamination in driving the formation of S pathology, we stereotaxically injected homozygous M83 S Tg mice in the hippocampus with 10 g of purified LPS, with an activity of ~0.']","Fig. 3 Immunofluorescence analysis of S pathology in M83 S Tg mice following injection of S fibrils. Representative immunofluorescent images of the cortical, hippocampal, and brainstem regions of hemizygous M83 S Tg mice injected with S or cation exchanged S fibrils, stained with antibodies to phosphorylated S (pSer129/81A; red) or S (SNL4; green) and DAPI (blue). Individual images were overlaid to show colocalization (Merge). Scale bar=50m",yes
PMC5342341,Figure_3,oa_package/84/fc/PMC5342341.tar.gz,['Microstructure defects in RFP mouse heartA.'],"Figure 3 Microstructure defects in RFP mouse heart Microstructural features of LV tissue sampled from 2-month-old wild-type and RFP+/+ mouse. (a) Wild-type myocardium shows organized myofilaments with clusters of normal mitochondrion aligned between myofibrils. (b-f) RFP+/+ myocardium: (b) Electron-dense residual bodies were detected among clustered mitochondrion with the contrast reduced. The myofilaments, although remain as organized array, appear less compact than wild-type myofilaments. (c) Widespread vacuoles between interrupted myofilaments. Large vacuoles contain damaged mitochondria. Degradation of mitochondrial matrix, disruption of cristae and lipid droplets was also shown. (d) Autophagic vacuoles with typical double membranes contains cytoplasmic material. (e) Large vacuoles of autophagosome (or lysosome) engulfed material of heterogeneous origins. (f) Macrophage in which the cytoplasm contains phagosomes, residual bodies and lysosomes with digested materials. A, autophagosome; L, lipid droplet; Ly, lysosome; M, mitochondria; N, nucleus; R, residual body; Z, Z-line. Black scale bar = 2 m; white scale bar = 0.5 m. The protein of EGFP, RFP, p62, LC3-I and LC3-II, LAMP1, and actin in 2- month-old wild-type, CALELD, RFP+/, and RFP+/+ heart. Representative images are shown. p62 and LC3 in wild-type, RFP+/, and RFP+/+ heart at 4 h after receiving intraperitoneal injection of 10 mg/Kg chloroquine (CQ) or saline. Representative images are shown. RFP/LAMP1/DAPI, RFP/LC3B/DAPI, and RFP/p62/DAPI triple staining on 2-month-old RFP+/+ heart sections. Top, white dashed line circled a large area containing large RFP aggregates but absent of LAMP1 staining. In contrast, LC3B and p62 are mostly colocalized with large DsRed aggregates (asterisks). Scale bar = 25 m.",yes
PMC10390345,Figure_4,oa_package/86/3c/PMC10390345.tar.gz,"['Intraoperative image showing large perforation at the lesser curvature of the stomach (the white elliptical area)The gastric mucosa was hemorrhagic (totaling almost 1300ml blood loss) and the cardia, fundus, and body of the stomach were noted to be gangrenous.']",Figure 4 Intraoperative image showing large perforation at the lesser curvature of the stomach (the white elliptical area),yes
PMC7322480,Figure_1,oa_package/cc/84/PMC7322480.tar.gz,"['Fundoscopy allows inspection of the optic disc (papilloedema and optic atrophy are easily appreciated; 1, 2\n) and retinal vessels, which may harbour retinal emboli or have bled.', ' 1Papilloedema.']",Figure1 Papilloedema.,yes
PMC10569753,Figure_1,oa_package/c9/f3/PMC10569753.tar.gz,['2 mg/dLTransverse section from the initial brain CT without contrast done in the emergency department showing a right occipital hematoma measuring 1.'],Figure 1 Transverse section from the initial brain CT without contrast done in the emergency department showing a right occipital hematoma measuring 1.1 x 1.8 x 1.6 cm (3.2 mL),yes
PMC11010354,Figure_2,oa_package/2e/45/PMC11010354.tar.gz,"[' 2).', '\nSoftware images of respiratory cases the software can display transaxial and sagittal images.', '782 cm3) and density (-104 Hu) of the lesions, and presented the suggestions on the follow-up to the lesions\n\nSoftware images of head and neck vascular cases.']","Fig. 2 Software images of respiratory cases the software can display transaxial and sagittal images. The AI circles marked the position (dorsal inferior lobe of right lung), size (1.4cm1.2cm), volume (1.782 cm ) and density (-104 Hu) of the lesions, and presented the suggestions on the follow-up to the lesions",yes
PMC4457974,Figure_3,oa_package/70/aa/PMC4457974.tar.gz,"[' 3a, b).', 'Effects of macrophage soluble factors on primary astrocytes.', 'c MTT assays on macrophage-stimulated astrocytes again show a proliferative effect of M1-, but not M2-, conditioned mediumM1 and M2 macrophages have opposite effects on astrocyte reactivityThe reactive marker expression of primary astrocytes was investigated by qPCR for genes known to be strongly upregulated in reactive astrocytes [26].']","Fig. 3 Effects of macrophage soluble factors on primary astrocytes. Primary astrocytes incubated with control or macrophage-conditioned medium show high levels of cell division as seen by EdU incorporation. Quantification of number of EdU+ cells shows a significantly higher percentage of cell division in M1- but not M2-stimulated cells at all time points. MTT assays on macrophage-stimulated astrocytes again show a proliferative effect of M1-, but not M2-, conditioned medium",yes
PMC8790214,Figure_3,oa_package/1d/b3/PMC8790214.tar.gz,"['One example of treatment effect is shown at baseline () and 16 weeks (', '16w after Dupilumab treatment (SCORAD 16).']",Figure 3 Baseline state on one patient in our series (SCORAD 64).,yes
PMC8626234,Figure_3,oa_package/09/c0/PMC8626234.tar.gz,"['A brain MRI performed 15 days after full recovery, was completely normal (\n).', 'Brain MRI, Axial FLAIR-weighted image, showing the disappearance of the hyperintense lesions previously noted.']","Fig. 3 Brain MRI, Axial FLAIR-weighted image, showing the disappearance of the hyperintense lesions previously noted.",yes
PMC4422728,Figure_4,oa_package/eb/97/PMC4422728.tar.gz,"['s002"" ref-type=""supplementary-material"">S2D Fig), 139 5 seemed to recognize only dense-core plaques (D; .05. Each group of animals was composed of 10 rats SpragueDawley rats and compared using analysis of variance followed by the MannWhitney or Dunnett test for group pairwise comparisons. Data represent mean SEM; NS, not significant; *, < .05; **, < .01; ***, < .001.",yes
PMC6234750,Figure_2,oa_package/fa/f8/PMC6234750.tar.gz,['MRI of endometrial cancer with cervical involvement.'],Figure 2 MRI of endometrial cancer with cervical involvement.,yes
PMC5582204,Figure_5,oa_package/74/cc/PMC5582204.tar.gz,['Circular gastric ulcer.'],Figure 5 Circular gastric ulcer.,yes
PMC8328747,Figure_3,oa_package/ee/70/PMC8328747.tar.gz,"['org/1999/xlink"" xlink:href=""10-1055-s-0041-1729130_8_3720_02""/>\nMusculocutaneous nerve neurogenic tumor.']",Fig. 3 Musculocutaneous nerve neurogenic tumor. Sagittal ultrasound image ( ) at distal arm shows a well-defined hypoechoic lesion (asterisk) along the musculocutaneous nerve. Axial T2-weighted ( ) and sagittal postcontrast T1-weighted ( ) magnetic resonance imagings show the well-defined intensely enhancing oval lesion (arrow in B and C) along the course of musculocutaneous nerve between the brachialis and biceps brachii muscles.,yes
PMC6050042,Figure_6,oa_package/69/34/PMC6050042.tar.gz,"['We then deleted each of the seven regions defined in this manner, including two that had been identified as controlling nuclear localization in previous studies, and assessed their localization by transfection in rodent primary cortical neurons followed by fluorescence microscopy (A).', '10.', '013.', '10.', '015 source data 1.', 'Survival data for E and nucleocytoplasmic localization data for F and figure supplement 1B.', '10.', '014 figure supplement 1.', 'Deletions of putative NLS (pNLS) 1, 2, 3, 5, 6, and 7 had little to no effect on neuronal MATR3 distribution (B).', 'These studies demonstrated that only the N-terminal arm is necessary for nuclear localization, as MATR3( pNLS4N)-EGFP exhibits nuclear clearing and punctate distribution in the cytoplasm and neuronal processes, while MATR3( pNLS4C)-EGFP has the same distribution as MATR3(WT)-EGFP (C D).', 'To test whether pNLS4N was sufficient for nuclear localization, we generated a construct in which the eight amino acids corresponding to pNLS4N were appended to EGFP, and compared the subcellular distribution of this construct in primary neurons to EGFP alone or EGFP fused to the canonical NLS from the SV40 large T antigen ( figure supplement 1) (Kalderon et al.', 'Automated survival analysis of neuronal populations expressing these constructs demonstrated that the pNLS4N mutation and resulting cytoplasmic localization significantly reduced MATR3-dependent toxicity compared to the MATR3(WT)-EGFP (E).', 'com/elifesciences-publications/nuclear-fractionation) to draw ROIs around the nucleus and soma of each neuron, measure MATR3-EGFP content separately within each compartment, and calculate a nucleocytoplasmic ratio for MATR3-EGFP in individual cells (F).', 'In agreement with single-cell data from transfected primary neurons, we noted no difference in the nucleocytoplasmic distribution of any of the MATR3-EGFP variants tested here (G).', 'Even so, the magnitude of the effect was relatively small, making it unlikely that differences in protein turnover fully explain the reduced abundance of MATR3(S85C)-EGFP noted in cell lysates (G).', 'In stark contrast to mild conditions (G), harsher lysis resulted in equivalent levels of all MATR3 variants on Western blot, suggesting that the S85C mutation reduced MATR3 solubility (E).', 'We also observed small, mobile MATR3 granules in the cytoplasm and neuronal processes when the bipartite NLS was disrupted (D).', 'For example, while amino acids 701 718 are essential for nuclear localization of rat MATR3 in Ac2F cells, deletion of the homologous sequence (amino acids 701 720) in human MATR3 has no effect on neuronal distribution (B).', 'As depicted in A, the NLS4N sequence (designated pNLS4N in the revised manuscript) begins downstream of RRM2.']",10.7554/eLife.35977.011,yes
PMC10689209,Figure_2,oa_package/4c/9f/PMC10689209.tar.gz,['Coronary angiogram.'],Figure 2 Coronary angiogram. Arrow points at the distal right coronary artery with 99% stenosis of 12 mm length.,yes
PMC5467963,Figure_4,oa_package/16/bf/PMC5467963.tar.gz,['4 pav.'],4 pav. vairios hemafagocitozs formos K aspirate (7),yes
PMC7359998,Figure_2,oa_package/45/cd/PMC7359998.tar.gz,[' 2Overall survival according to clinical pathological features by sexOnly 1.'],Fig.2 Overall survival according to clinicalpathological features by sex,yes
PMC9815792,Figure_2,oa_package/6c/c5/PMC9815792.tar.gz,"['Arteriogram of the right external iliac with selective catheterization and embolization of the AUF between the grafted right pancreatic artery and ureter.', ""Coronal and Sagittal CT imaging of the patient's abdomen and pelvis following AUF embolization.""]","Fig. 2 Arteriogram of the right external iliac with selective catheterization and embolization of the AUF between the grafted right pancreatic artery and ureter. (a) The initial run of the right iliac arteriogram. (yellow arrow) pointing to the truncated pancreatic artery. (b) Yellow arrow demonstrates the persistence of the truncation with an additional run in closer proximity to the pancreatic artery after catheter retraction. (c) Selective catheterization was performed. Yellow arrow points out the narrow tract that moves towards the ureteral stent, correlating to the right ureter. (d) After additional contrast delivery, the contrast migrates cephalad to further outline the fistulous communication with the ureter. Yellow arrow points toward the distal area of migration. (e) Embolization coils are deployed distally within the tract. Yellow arrow shows contrast halting proximal to the tract. (f) Additional coils were placed proximally after distal coils were appropriately placed. Yellow arrow highlights the embolized tract, representing the prior communication between the pancreatic artery and the right ureter.",yes
PMC7239073,Figure_4,oa_package/09/20/PMC7239073.tar.gz,"['The learned behavior of the baseline trained VGG-16 model with the pediatric CXR collection is interpreted through Grad-CAM visualizations and is shown in .', 'Unlike the degraded performance of the model trained on non-augmented data that failed to localize salient ROI in a test CXR showing COVID-19 viral pneumonia, as observed from , the model trained on the augmented baseline with COVID-19 CXRs from one collection delivered superior localization performance with the test CXR samples from the other collection.', '20090803-f0003""/>.']",Figure 4. Original CXRs and their salient ROI visualization: ( ) and ( ) shows a CXR with bilateral bacterial pneumonia and the corresponding Grad-CAM visualization; ( ) and ( ) shows a CXR with viral pneumonia manifestations and the corresponding salient ROI visualization; ( ) and ( ) shows a sample CXR from the test set of Montreal COVID-19 CXR collection with GT annotations and the corresponding salient ROI visualization.,yes
PMC4531783,Figure_2,oa_package/d8/8a/PMC4531783.tar.gz,"['Hepatic steatosis and cellular ballooningLevels of fasting glucose were significantly elevated in ApoE / mice compared to wt littermates with normal chow (A).', 'By contrast, mice fed with MCD diet for seven weeks showed significantly decreased levels of serum fasting glucose (A).', 'Hepatic triglycerides were increased in mice fed with WD compared to mice fed with normal chow, with only a slight, but not significant, trend towards higher levels in ApoE / mice (B).', 'In mice fed with MCD diet were measured the highest levels of hepatic triglycerides (B).', 'The serum lipoprotein profiles showed profound changes between ApoE / mice and wt mice fed with WD, with a marked shift towards increased VLDL levels in ApoE / mice fed with WD (C).', 'ApoE / mice fed with WD experienced an increase in levels of total and free hepatic cholesterol compared to all other observed groups (D,E).', 'Components of cholesterol biosynthesis, as exemplary shown by total levels of desmosterol, were significantly increased in ApoE / mice fed with WD compared to wt littermates (F).', 'Hepatic steatosis was further quantified by Oil Red O staining, a histological marker for accumulation of fat in hepatocytes (G,H).', 'Comparing all groups, ApoE / mice fed with WD showed the highest amount of Oil Red O positive staining (G,H).', 'Compared to mice with normal chow, mice fed with MCD diet had significantly increased Oil Red O staining, however no differences between wt and ApoE / mice were found (G,H).', 'Interestingly, in ApoE / fed with WD microvesicular steatosis was predominant, while in mice fed with MCD diet mainly macrovesicular steatosis was observed (G).', 'org/1999/xlink"" xlink:href=""srep12931-f1""/>Hepatic steatosis in wt and ApoE / mice with or without WD or MCD diet.']","Figure 2 Hepatic steatosis in wt and ApoE mice with or without WD or MCD diet. ( ) Levels of fasting glucose. ApoE mice fed with Western Diet (WD) showed abnormal fasting glucose levels compared to animals fed with normal chow or methionine and choline deficient (MCD) diet. ( ) Hepatic triglyceride content expressed as percentage of wt mice. Levels of hepatic triglycerides were increased in ApoE mice fed with WD compared to mice fed with normal chow, but lower than in mice fed with MCD diet. ( ) Serum lipoprotein profiles of mice fed WD. ApoE mice fed with WD showed shift in serum lipoprotein profile with high amount of very low density lipoproteins (VLDL) compared to wt. Levels of total ( ) and free ( ) hepatic cholesterol. ApoE fed with WD showed increased levels of total and free hepatic cholesterol in comparison to the other observed groups. ( ) Desmosterol/cholesterol ratio was significantly increased in ApoE mice compared to the other observed groups. ( ) Representative sections and ( ) quantification (% of positive stained area) of Oil Red O histological staining, as marker of fat accumulation in the liver. ApoE mice fed WD showed significantly higher Oil Red O positive staining than wt mice. In mice fed with MCD diet slightly less Oil Red O positive staining was observed. Graphs are expressed as means standard deviation. The scale bar is 100m. p<0.05 was considered significant.",yes
PMC8994011,Figure_6,oa_package/1d/05/PMC8994011.tar.gz,"[' 6).', 'Large alveolar echinococcosis lesion of the right liver in a 34-year-old man diagnosed with jaundice.', 'Percutaneous biliary drainage to achieve regression of jaundice in preparation for liver surgerySerologyAE serology is efficient and is performed either to confirm the diagnosis, when it is suspected on imaging, or to rule out the diagnosis, if there is diagnostic uncertainty [10].', ' 6, 10).', ' 6).']",Fig. 6 Large alveolar echinococcosis lesion of the right liver in a 34-year-old man diagnosed with jaundice. CT image in the axial plane obtained at portal phase of enhancement showing alveolar echinococcosis of the right liver (arrow) associated with infiltration up to the right pedicle (arrowheads) and the biliary convergence. Coronal T2-weighted MR images. Multiple microcysts (arrowheads) with biliary convergence involvement leading to biliary dilation in the left lobe (arrow). Cholangiography images. Percutaneous biliary drainage to achieve regression of jaundice in preparation for liver surgery,yes
PMC10435640,Figure_3,oa_package/24/5a/PMC10435640.tar.gz,"[' 3) [5, 13, 21].', 'The four pulmonary veins: the pulmonary veins (*) drain the blood from the lungs, each through their own ostium in the posterior wall of the left atrium separately.', 'RA right atrium; LA left atrium; RV right ventricle; LV left ventricleThe extrapulmonary segments of the fetal pulmonary veins are supplied by nerves extending from three epicardiac ganglionated subplexuses: the left dorsal subplexus and the left and middle dorsal subplexuses [23].']","Fig. 3 The four pulmonary veins: the pulmonary veins (*) drain the blood from the lungs, each through their own ostium in the posterior wall of the left atrium separately. right atrium; left atrium; right ventricle; left ventricle",yes
PMC10509632,Figure_2,oa_package/b2/9c/PMC10509632.tar.gz,['Reconstruction of the mesenteric artery after resection of the tumorProcedure of bypass to intraperitoneal arterial blood flow after resection of the tumor.'],"Figure 2 Reconstruction of the mesenteric artery after resection of the tumor Procedure of bypass to intraperitoneal arterial blood flow after resection of the tumor. Categorical data are presented as number (%). CHA, common hepatic artery; SA, splenic artery; LGA, left gastric artery; SMA, superior mesenteric artery",yes
PMC9805223,Figure_1,oa_package/aa/5f/PMC9805223.tar.gz,"['Primary melanoma tumor of the leg.', '1Repositionable guidewire device.']","Figure 1 Primary melanoma tumor of the leg. Clinical photograph ( ). Highresolution, Bmode scan showing a homogeneously, hypoechoic thick lesion ( ). Superb microvascular imaging scan demonstrating an intense tumor vascularization ( ).",yes
PMC9649549,Figure_1,oa_package/c0/95/PMC9649549.tar.gz,['\nContrast-enhanced computed tomography and contrast-enhanced magnetic resonance imaging reveal multiple low-density lesions in the liver with slightly low enhancement.'],Figure 1 A: Contrast-enhanced computed tomography; B: Contrast-enhanced magnetic resonance imaging.,yes
PMC6034824,Figure_5,oa_package/6f/70/PMC6034824.tar.gz,"['Scoring of histopathological changes in the gillsGill lamellar thrombiLamellar thrombi () were seen in the gills from 1 dphe onwards; thrombi scores ranged from 0 to 3.', 'g005Gill filament of Atlantic salmon showing a lamellar thrombus after exposure to hydroids (H E and MSB stained).']",10.1371/journal.pone.0199842.g005,yes
PMC10728248,Figure_7,oa_package/74/9d/PMC10728248.tar.gz,"[' 7).', 'Ex vivo microscopic images.']","Fig. 7 Comparison of fluorescence signals between compounds. Images of the brains of 1112month-old amyloid- precursor protein knock-in (APP-KI) mice acquired after intravenous administration of THK-565, THK-265, and vehicle. Fluorescence signal intensities of the heads of 1112month-old APP-KI mice as a function of time after the intravenous administration of THK-565, THK-265, or vehicle. Average fluorescence intensity of THK-565, THK-265, and vehicle between 0 and 60min after injection in APP-KI mice.",yes
PMC10976582,Figure_3,oa_package/87/d4/PMC10976582.tar.gz,['Best practices for femoral arterial access and potential vascular complications.'],"Figure 3 Best practices for femoral arterial access and potential vascular complications. ( ) The optimal technique for ultrasound-guided femoral arterial access requires the use of a 5- to 10-MHz linear ultrasound probe which is held such that the vertical marker is facing upward from the femoral artery to facilitate a longitudinal view of the common femoral artery and its branches. The access needle should then be used at a 45 degree angle to access the common femoral artery. ( ) Longitudinal view of the femoral vasculature demonstrates the posterior dive that the common femoral artery takes below the inguinal ligament as the probe is advanced cranially. ( ) Rotating the ultrasound proble 90 degrees allows for cross sectional view of the femoral bifurcation, including anterior or posterior vessel wall calcification. ( ) Vessel puncture cranial to the inferior epigastric artery is associated with increased risk for retroperitoneal bleeds. ( ) Vessel puncture below the common femoral artery bifurcation and involving the superfical femoral artery is associated with increased for pseudoaneurysm formation. Pseudoaneurysms can present clinically as pulsatile hematomas, painful ecchmyoses or as frank bleeding. They typically consist of 1 or 2 layers of the vessel wall, and are therefore associated with increased risk for rupture, embolization, and limb ischemia. They typically demonstrate to-and-fro patterns of flow with pulsed Doppler assessment.",yes
PMC6511210,Figure_4,oa_package/57/94/PMC6511210.tar.gz,"['4a.', '4b.', 'a.', 'The image of removed inferior vena cava and right atrium thrombosis after the operationDiscussion and conclusionPheochromocytoma represents very significant challenges to the anesthetist, which are not uncommon in anesthetic practice.']",Fig. 4 . The image of resected gigantic pheochromocytoma. . The image of removed inferior vena cava and right atrium thrombosis after the operation,yes
PMC11476796,Figure_5,oa_package/bc/15/PMC11476796.tar.gz,"['While the effect was compatible between quercetin mono-treatment and albendazole mono-treatment, a better response was seen in the albendazole-quercetin co-therapy group compared to that in the albendazole or quercetin monotherapy groups (A and 5B).', 'Apoptotic neurons were also decreased in different brain parts, with mice receiving albendazole-quercetin co-treatment showing the most significant improvement (A and 5C).', 'While albendazole and quercetin mono-treatment have minimal effect against neuronal cell death, albendazole-quercetin co-therapy resulted in better, dose-dependent protection of the neurons (D and 5E).', 'g005Brain histopathology of mice treated with albendazole and different doses of quercetin.', 'g005"" ref-type=""fig"">).', 'This result was similar to the results of the Nissl-stained sections (D and 5E).', 'Therefore, the suppression of brain IL-5 levels may suggest a lesser eosinophils recruitment and, thereby, lesser inflammation, corroborating what we have seen in the histology ().']",10.1371/journal.pntd.0012526.g005,yes
PMC9114469,Figure_10,oa_package/8c/dc/PMC9114469.tar.gz,[],Figure 10 Trunk torsion.,yes
PMC7699768,Figure_16,oa_package/33/a2/PMC7699768.tar.gz,[],"Figure 16 Computed tomography pulmonary angiography (CTPA) in the detection of main pulmonary embolism with different scanning protocols. ( , ) When pitch was increased to 3.2, image noise was increased with 70 and 80 kVp protocols; ( , ) in contrast, no significant change of image quality was noted with 100 and 120 kVp protocols, regardless of pitch values. Arrows refer to the thrombus in the main pulmonary arteries. Reprinted with permission under open access from Aldosari et al. [ ].",yes
PMC4275226,Figure_8,oa_package/37/c7/PMC4275226.tar.gz,"['Incidence and severity as well as time of onset of CIA were similar in WT and CRAMP / mice (A C).', 'Histomorphometry revealed no differences between CRAMP+/+ and CRAMP / mice regarding inflammation area, cartilage degradation, or bone erosions (D E).', 'g008Comparison of collagen-induced arthritis (CIA) in WT and CRAMP-deficient mice.']",10.1371/journal.pone.0115474.g008,yes
PMC7583720,Figure_1,oa_package/a2/91/PMC7583720.tar.gz,"['There was a significant induction of cytokines like IL-12 (AI) and TNF-alpha (AII) in macrophages, and as expected in negative controls, cytokine levels were at best negligible.', '7 macrophage cells and RAW- TLR2 (A).', 'Rv1954A enhances TLR4-mediated production of pro-inflammatory cytokines in macrophages.', '7 and RAW- TLR2 cells with Ms_Rv1954A compared to Ms_Vc (B).', 'When there was no rRv1954A protein added, such red puncta were not observed (C, row 1).', '7 cells and RAW- TLR2 incubated with Rv1954A protein (C, row 2 and row 3).', 'However, these structures were not observed on the surface of RAW- TLR4 cells and RAW- TLR2 TLR4 cells even in the presence of Rv1954A protein (C, row 4 and row 5).', 'Pretreatment of anti-TLR4 resulted in no complex formation on the surface of the cells (D, row 1) whereas no pretreatment of anti-TLR4 resulted in complex formation (D, row 2).']","Figure 1 Rv1954A enhances TLR4-mediated production of pro-inflammatory cytokines in macrophages. RAW264.7, RAW-TLR4, and RAW-TLR2 cells were treated with purified Rv1954A protein (2, 5, 10 g/ml). Autoclaved (AC) protein and proteinase K (PK)-treated protein served as negative controls. LPS treatment served as a positive control. Levels of IL-12 and TNF- were estimated using ELISA . Representative data from three experiments show the concentration of IL-12 and TNF- as mean SEM. Statistical significance was determined with the student -test. RAW264.7, RAW-TLR4, and RAW-TLR2 were infected with Ms_Vc and Ms_Rv1954A at an MOI of 1:10. Supernatants were collected after 24 h of infection, and secretion of cytokine levels was estimated through ELISA. Representative data from three experiments show the concentration of IL-12 and TNF- as mean SEM. Statistical significance was determined with the student -test. RAW264.7, RAW-TLR4, RAW-TLR2, and RAW-TLR2TLR4 cells were cultured on coverslips followed by incubation with Rv1954A protein 10 g/ml for 6 h. The cells were fixed and incubated with anti-Rv1954A followed by staining with Alexa Flour 594 nm and DAPI and visualized by a fluorescent microscope (magnification is 40, scale bar represents 20 m) . RAW 264.7 cultured on the coverslips were pretreated with or without rat anti-mouse anti-TLR4 for 90 min. Cells were stimulated with 10 g/ml rRv1954A protein for 6 h followed by fixing and treatment with anti-Rv1954A antibody raised in rabbit tagged with Alexa Flour 594 and DAPI followed by mounting and visualization under a fluorescent microscope (magnification is 40, scale bar represents 20 m) .",yes
PMC4150474,Figure_5,oa_package/a3/6d/PMC4150474.tar.gz,"['Results showed that the dendritic spine density of the frontal cortex decreased in the diabetic rats at 4 months after STZ injection compared with that in the age-matched control rats (s 5(a) and 5(c)).', 'Consistently, the synaptophysin expression of the frontal cortex was lower in the diabetic rats than in the control rats (s 5(d) and 5(f)).', 'Similarly, the dendritic spine density of the hippocampus decreased in the diabetic rats at 4 months after STZ injection compared with that in the age-matched rats (s 5(b) and 5(c)).', 'The synaptophysin level in the hippocampal tissues decreased in the diabetic rats compared with that in the age-matched control rats (s 5(e) and 5(f)).', '004""/>Decline in dendritic spine density in the frontal cortex and hippocampus of rats at 4 months after STZ injection.']","Figure 5 Decline in dendritic spine density in the frontal cortex and hippocampus of rats at 4 months after STZ injection. (a) Representative images of the dendritic spine of the pyramidal neurons in cortical layers II/III. (b) Representative images of the dendritic spine of the pyramidal neurons in the CA1 region of the hippocampus. (c) Dendritic spine density in the frontal cortex and hippocampus was measured (see Materials and Methods for procedure details). * < 0.05, significant difference compared with the age-matched control rats; = 4. (d) Representative images of synaptophysin expression in the frontal cortex using western blot analysis. (e) Representative images of synaptophysin expression in the hippocampus using western blot analysis. (f) Relative expression of synaptophysin in the frontal cortex and hippocampus was determined. * < 0.05, significant difference compared with the age-matched control group; = 4.",yes
PMC11519952,Figure_6,oa_package/2a/10/PMC11519952.tar.gz,"[' 6a displays five nodules that demonstrate complete agreement with the sonographer s diagnosis.', ' 6b.', ' 6c).', ' 6b, the nodule s composition is categorized as solid, leading to a score of 2, while its echogenicity is classified as hypo, also resulting in a score of 2.', 'Scoring and classification of thyroid nodules based on ACR-TIRADS.', 'According to doctor s evaluation, 17 individuals were found to have nodules, while 2 individuals showed no presence of nodules.', ' 6b illustrates a sample outcome obtained from the ACR-TIRADS calculation:Echogenicity, composition and echogenic foci: to assess the echogenicity, composition, and echogenic foci of the thyroid nodule, we analyze the distribution of pixels in the thyroid gland and the nodule, as depicted in ', ' 6c.']","Fig. 6 Scoring and classification of thyroid nodules based on ACR-TIRADS. Indicative nodules identified with FARUS and the respective US images provided by doctors. Examples of two separate nodules, with accompanying explanations of their TIRADS scores. Correlation between number of pixels over brightness in thyroid image. Source data are provided as a Source Data file.",yes
PMC5535563,Figure_2,oa_package/37/ae/PMC5535563.tar.gz,['Lateral 3D-CT study showing grade I spondylolisthesis at the L4 L5 level with marked degenerative changes involving the L4 L5 facet joint and narrowing of the L4 L5 foramenAxial illustration (Joseph A.'],Figure 2 Lateral 3D-CT study showing grade I spondylolisthesis at the L4L5 level with marked degenerative changes involving the L4L5 facet joint and narrowing of the L4L5 foramen,yes
PMC7821587,Figure_7,oa_package/8e/d5/PMC7821587.tar.gz,"['After 2 hours of being exposed to CPB condition, THP-1 cells were changed to fresh media, and strikingly, the cell death could be rescued; the percentage of live cells almost doubled, while the percentage of necrotic cells drastically reduced (, A and B).', 'Removing the remnants from the sheared media or adding the remnants to the fresh media did not alter the percentage of cell death (B).', 'Indeed, naive static THP-1 cells treated with the sheared media, with and without the remnants, experienced a marked increase in cell death (Supplemental , A and B).', 'Sheared THP-1 cells treated with the humanized TNF- antibody, equivalent to adalimumab (Humira), experienced approximately 24% less necrotic cell death compared with the cells treated with the isotype control antibody (C).', 'CRISPR/Cas9-mediated KO of TNFR1, the receptor mediating cellular effects of TNF- , reduced necrotic cell death by approximately 41% (D) and decreased cleaved caspase-3 and p-RIPK1 levels (E).', 'Furthermore, when naive static THP-1 cells were treated with human recombinant IL-8, TNF- , or both for 24 hours, the combination of IL-8 and TNF- induced more cell death than TNF- treatment alone, as shown by the cellular morphology (Supplemental C) and confirmed by the increased levels of cleaved caspase-3, p-RIPK1, and p-RIPK3 (Supplemental D).', 'CPB-induced necroptosis is partly mediated by TNF- .']","Figure 7 CPB-induced necroptosis is partly mediated by TNF-. THP-1 cells were subjected to CPB conditions for 2 hours, recovered for 24 hours, and cell death was analyzed by flow cytometry. ( and ) When sheared THP-1 cells were changed to fresh media, the percentage of live THP-1 cells significantly increased, while the percentage of necrotic cells significantly decreased. Removing insoluble remnants of ruptured cells from the sheared media was not sufficient to rescue the cell death, while adding the remnants to the fresh media did not change the percentage of live or necrotic THP-1 cells ( = 3 replicates/group). ( ) Treatment of sheared THP-1 cells with humanized TNF- neutralizing antibody significantly increased the percentage of live cells and reduced the percentages of both early apoptotic and necrotic cells ( = 3 replicates/group). ( ) CRISPR/Cas9-mediated knocking out of TNFR1 receptor significantly increased the percentage of live cells and reduced the percentages of both early apoptotic and necrotic cells compared with the control cells ( = 3 replicates/group). ( ) Western blot showed reduction of cleaved caspase-3 and p-RIPK1 in sheared TNFR1-KO cells compared with control cells. These blots were run in parallel and contemporaneously on separate gels. * < 0.05, 1-way ANOVA and post hoc Dunnetts test ( ), 2-tailed Students test ( and ).",yes
PMC8311746,Figure_4,oa_package/16/f6/PMC8311746.tar.gz,"['Ulnar nerve gap was restored by harvesting the homolateral sural nerve, which was duplicated and end-to-end sutured, covered by the amniotic membrane ().']","Figs 4A to G The reconstructive orthoplastic strategy was planned: the primary purpose was to provide an adequate soft tissue and skin coverage. The patient underwent plastic surgery to provide soft tissue coverage with a musculocutaneous latissimus dorsi rotational flap. (A). After VAC and hyperbaric therapy, a good and clean granulation tissue was achieved and definitive skin coverage could be provided. Skin elbow situation before skin coverage surgery; (B). Ulnar nerve gap (7 cm); (C). Harvesting of homolateral sural nerve; (D). Ulnar nerve gap was restored by harvesting homolateral sural nerve, which was duplicated and sutured end to end using microsurgical technique. It was covered by amniotic membrane; (E). Musculocutaneous latissimus dorsi rotational flap preoperative drawing; (F). Musculocutaneous latissimus dorsi rotational flap was harvested and rotated to cover the receiving area; (G). Final outcome",yes
PMC11717497,Figure_4,oa_package/36/1b/PMC11717497.tar.gz,"['Representative images and quantification of positive area (%) of Gpx4 (A, B) and 4HNE (C, D) of thoracic aortic sections for CKD vehicle, 3 mg/kg iron and 10 mg/kg iron treated CKD rats.']","Figure 4 Representative images and quantification of positive area (%) of Gpx4 (A, B) and 4HNE (C, D) of thoracic aortic sections for CKD vehicle, 3mg/kg iron and 10mg/kg iron treated CKD rats. Gpx4 and 4HNE positivity is shown as brown deposits. Data are presented as individual values (dots) and median (line). KruskalWallis with Dunn's multiple correction for the CKD groups versus CKD vehicle group. Significance versus CKD vehicle group: * <0.05. Correlation between (E) Gpx4 expression and (F) 4HNE presence versus % calcified area (on Von Kossastained aortic sections) in iron (left graph) and vehicle treated CKD rats (right graph). Spearman's correlation analysis and simple linear regression were performed. =Spearman's rank correlation coefficient. 4HNE, 4hydroxy2nonenal; CKD, chronic kidney disease; Fe, iron, Gpx4, glutathione peroxidase 4; veh, vehicle.",yes
PMC5475416,Figure_5,oa_package/11/80/PMC5475416.tar.gz,"['s 3 shows the aesthetic outcome with periocular rhytides after four HPPL /IFL sessions; s 4 the rapid improvement of skin texture after four sessions of HPPL /IFL skin rejuvenation and six weeks of follow-up; the dramatic long-term improvement of dermal atrophy in a patient with severely excoriated acne as result of repeated picking at skin inflammatory blemishes.', '.']","Figure 5. Improvement of dermal atrophy in an adolescent girl with excoriated acne, monthly HPPL/IFL treatment, 5 sessions. Settings: 3 single-flash passages, 35-45 msec, 30-55 J; cut-off filters at different passages, 520, 590 and 650 nm.",yes
PMC7185769,Figure_15,oa_package/f2/8f/PMC7185769.tar.gz,[],"Fig.15 Aspiration pneumonia with severe, multiple, necrotic cavitations.",yes
PMC6098207,Figure_2,oa_package/1a/cc/PMC6098207.tar.gz,"['Radiographic honeycombing was present in 6/14 (43%) and traction bronchiectasis was seen in 8/14 (57%) (A).', 'Ground glass opacities was the most frequently observed radiographic finding, present in 13/14 (93%) (B).', 'Small nodules were common, found in 9/14 (64%) (C).', 'Small cysts were uncommon and found in only 3/14 (21%) (D).', 'Radiographic and pathologic manifestations of hard metal pneumoconiosis.', 'The expressions of RANK, RANKL, BMP2, BMP7, RUNX2, and OPG were visualized by Western blotting (3).', '05; 4 A,B).', '05; 4 C,D,E,F).', 'g0133Western blot results.', 'g0144Quantitative results of Western blot analysis for the expression of RANK, RANKL, BMP2, BMP7, RUNX2, and OPG.']",10.1371/journal.pone.0096361.g001,yes
PMC8601817,Figure_2,oa_package/f4/21/PMC8601817.tar.gz,"['001"" position=""float""/>TTC.']","Figure 2 TTC. (a) Representative TTC staining of rat brain slices. Unstained areas indicate tissue damage. (b) QSW alleviates cerebral infarction. The data were presented as meansSD; =6 rats per group. Compared with the I/R group, < 0.05 and < 0.01.",yes
PMC10516498,Figure_4,oa_package/12/41/PMC10516498.tar.gz,[],"Fig.4. Comparison of SARS-CoV-2 Omicron BA.1 and BA.5 genome sequences. ( ) Full genome comparison of Omicron BA.5 to BA.1. In red are amino acid differences, ** represent mutations unique to our viral stock, and represent a deletion. ( ) S protein comparison between Omicron BA.5 and BA.1. ( ) Omicron BA.1 S protein [structure source Protein Data Bank (pdb): 7tgw]. Blue, NTD; grey, RBD; yellow, Receptor-binding motif; red, mutated residues in Omicron BA.5.",yes
PMC5467773,Figure_3,oa_package/5a/18/PMC5467773.tar.gz,['High-resolution computed tomography (HRCT) showing fibrosis and scarring more marked on the left than on rightDiscussionThe diagnosis of drug-induced lung disease (DILD) depends upon establishing a definite temporal relation between exposure to the causative agent and the development of the respiratory symptoms and signs.'],Figure 3 High-resolution computed tomography (HRCT) showing fibrosis and scarring more marked on the left than on right,yes
PMC8671092,Figure_6,oa_package/76/7f/PMC8671092.tar.gz,"['The brain CT scan showed subarachnoid bleeding ().', 'Right fronto basilar subarachnoid hemorrhage with ventricular flooding.', 'The conduct was stopping anticoagulants with neurological monitoring.']",Fig. 6 Right fronto basilar subarachnoid hemorrhage with ventricular flooding.,yes
PMC10747232,Figure_9,oa_package/60/ea/PMC10747232.tar.gz,"['The results showed that all four kinds of probiotics, Bifidobacterium, Limosilactobacillus, Adlercreutzia, and Akkermansia, were positively correlated with the results of the water maze test, suggesting that the relative abundance of intestinal probiotics in APP/PS1 mice was significantly increased after EGb treatment, which further affected their learning and memory behavior, and there was a correlation between intestinal probiotics and cognitive dysfunction ().', 'Correlation between gut microbiota and behavior results.']","Figure 9 Correlation between gut microbiota and behavior results. Correlation analysis between ( ) , ( ) , ( ) , and ( ) and time in target quadrant of APP/PS1 mice before and after EGb treatment.",yes
PMC7653202,Figure_1,oa_package/73/5f/PMC7653202.tar.gz,"['His 12-lead electrocardiogram (A) showed deep symmetrical T wave inversions at the inferior leads.', 'The right sided coronary angiogram (B) depicted occlusion at the mid segment of the right coronary artery.', 'A subsequent carotid angiogram (C) illustrated a 1.', '(A) 12 lead electrocardiogram showed deep symmetrical T wave inversion at the inferior leads.', 'At the cardiology department, a carotid wallstent was deployed to the left internal carotid artery by the interventional cardiologist.', 'The peripheral blood film (D) was consistent with hemolytic anaemia.']","Fig. 1 (A) 12 lead electrocardiogram showed deep symmetrical T wave inversion at the inferior leads. Right sided cardiac angiogram showed occlusion at mid right coronary artery. Carotid angiogram showed left internal carotid artery thrombus. Peripheral blood film showed polychromasia, reticulocytosis and microspherocytes.",yes
PMC6775665,Figure_6,oa_package/72/89/PMC6775665.tar.gz,"[' 6).', '', '01\nThe expression of 209 miRNAs was significantly different in tumors according to size, using 20 mm in diameter as cutoff (Additional file 1: Table S1).']","Fig.6 Expression levels of miRNAs in the miR-17-92 and miR-106b-25 clusters according to molecular subtype. MiRNA expression in breast cancer and benign tissue is presented with microarray expression levels given as mean (standard error) of log2 transformed intensity values (Hy3). *False discovery rate (FDR) adjusted -value<0.05, ** -value<0.01",yes
PMC11084666,Figure_1,oa_package/5a/e3/PMC11084666.tar.gz,"['In summary, it was found that the activation of the GLP1R alleviated apoptosis, necroptosis, ferroptosis, and pyroptosis ().', '1139/cjpp-2022-053436848640\nThe mechanism of GLP-1-induced prevention of myocardial remodeling.']","Figure 1 The mechanism of GLP-1-induced prevention of myocardial remodeling. DPP4, dipeptidyl peptidase 4; AT1R, angiotensin-1 receptor; GLP1R, glucagon-like peptide-1 receptor; K , ATP sensitive potassium channel; FGF-2, fibroblast growth factor-2; MKK3, mitogen-activated protein kinase kinase-3; MEK1/2, mitogen-activated protein kinase kinase-1/2; MMP2/9, matrix metalloproteinase 2/9; GSK3, kinase glycogen synthase 3 ; Smad3, Suppressor of Mothers Against Decapentaplegic 3; PPAR-/, peroxisome proliferator-activated receptor; Nrf2, nuclear factor erythroid 2-related factor 2; CREB, cAMP response element-binding protein; STAT3, signal transducer and activator of transcription 3; SIRT-1, silent information regulator-1; PARP1, poly(ADP-ribose) polymerase 1; MPT-pore, mitochondrial permeability transition pore.",yes
PMC7600008,Figure_3,oa_package/ed/92/PMC7600008.tar.gz,"['Giant cells containing multiple nuclei and reorganization of the actin cytoskeleton were noticed in the Cbl-deficient culture, unlike the control (A).', 'As presented in B, cobalamin deficiency in cultured astrocytes was related to a significant increase in cell size.', 'Hypovitaminosis B12 results in astrocytes hypertrophy.']","Figure 3 Hypovitaminosis B12 results in astrocytes hypertrophy. ( ) Representative confocal images of control and cobalamin-deficient normal human astrocytes; arrows indicate multinucleation; scale bar shows 100 m. ( ) Cell size (area) was evaluated from the confocal images. Data acquired from at least three independent experiments are presented as box plots. The boxes show, from bottom to top, the 25th percentile, median, and 75th percentile values, and the whiskers indicate the maximum and minimum values. More than 300 cells from different randomly selected microscope fields are analyzed for each condition. The cells that were fully visible were bordered manually and the area of the cell body was determined using ImageJ software. Statistically significant differences are designated as ** < 0.01 for comparisons between groups using unpaired -test.",yes
PMC11452557,Figure_18,oa_package/0e/c4/PMC11452557.tar.gz,[],Fig. 18 Interleukin 6 (IL-6).,yes
PMC4270229,Figure_1,oa_package/25/0f/PMC4270229.tar.gz,[],Figure 1. Morphometric analysis demonstrating marked collagen fiber deposition and SMAexpressing myofibroblasts within the lungs of bleomycinchallenged rats. Sections of lung tissue isolated from groups of saline or bleomycinchallenged rats ( = 8) at various time points were stained with either PicroSirius red (PSR) for collagen or with a SMAspecific antibody to detect the presence of contractile myofibroblasts within fibrotic foci. Sections were counterstained blue with hematoxylin. Representative samples are shown at 10 and 20 magnification.,yes
PMC11412753,Figure_3,oa_package/92/ef/PMC11412753.tar.gz,"['Surgical InterventionFollowing a thorough diagnostic arthroscopy, the patient was found to have medial and lateral meniscal root tears as well as a midsubstance ACL tear (s 3(a), 3(b), and 3(c)).', '002"" position=""float""/>Arthroscopic view from the anterolateral portal of the (a) medial and (b) lateral meniscal root tears and (c) a midsubstance ACL tear.']",Figure 3 Arthroscopic view from the anterolateral portal of the (a) medial and (b) lateral meniscal root tears and (c) a midsubstance ACL tear.,yes
PMC9327355,Figure_3,oa_package/9d/42/PMC9327355.tar.gz,"['The technique of closing the PPF is the same as that of ECF closure ().', '.']","Fig. 3. Closure of a postoperative pancreatic fistula A. Enhanced computed tomography (CT) performed 9 days after pancreaticoduodenectomy showed fluid collection at the pancreatojejunal anastomosis (arrow). B. Percutaneous drainage was performed, but pancreatic juice was continuously discharged through the catheter, although fistulography showed no opacification of the pancreatic duct. C. The discharge continued and enhanced CT performed 43 days after surgery showed a small amount of fluid at the anastomosis site (arrow). The arrowhead indicates the drainage catheter. D. On day 50 after surgery, a fibrin glue was injected into the fistula using two 4-F catheters. The fistula was closed after the procedure. E. Unenhanced CT performed 1 month after the procedure showed granulation at the anastomosis site (arrow).",yes
PMC6238660,Figure_5,oa_package/41/89/PMC6238660.tar.gz,['Immunization with different A.'],"Figure 5 Immunization with different extracts had distinct effects on leukocyte influx into the airways following infection. Inflammatory cells in the bronchoalveolar lavage (BAL) after immunization with the extracts and challenge with were quantified using a Neubauer chamber and cytospin preparations. Total leukocytes. Eosinophils. Neutrophils. Macrophages. Lymphocytes. Total protein. Statistically significant differences ( < 0.05) were plotted compared to the control groups PBS-NI and PBS-ASC in which < 0.05, < 0.01, < 0.001.",yes
PMC5572024,Figure_1,oa_package/e4/56/PMC5572024.tar.gz,['A nodule (early imaging standardized uptake value [SUV]max = 2.'],"Figure 1 A nodule (early imaging standardized uptake value [SUV] =2.80, delayed imaging SUV =5.51) was detected in the middle lobe of right lung on the PET-CT. This nodule is quite difficult to distinguish with other pulmonary nodules on CT scan. CT=computed tomography, PET-CT=positron emission tomography-computed tomography.",yes
PMC4869455,Figure_15,oa_package/15/5f/PMC4869455.tar.gz,[],"Figure 15 Histopathological picture of phakomatous choristoma showing cataractous lens tissue surrounded by epithelium, embedded in fibrous tissue (H and E, 40)",yes
PMC5984465,Figure_4,oa_package/d2/83/PMC5984465.tar.gz,"[' 4b, e, f and 5) were performed.', ' 4a, b).', '4c, d), MAP2 area (', '4c, e) or neuron number (', '4c, f).', 'LRRK2 inhibition does not reduce pathological -synuclein in wildtype hippocampal neurons.', 'Scale bars = 50 mWe then tested whether inhibition of LRRK2 activity can alter -synuclein pathology induced by a means other than PFFs.']","Fig. 4 LRRK2 inhibition does not reduce pathological -synuclein in wildtype hippocampal neurons. Primary cortical neurons were treated with LRRK2 inhibitors PF-475, PF-360, or MLi-2, at 5 DIV and fed with media containing inhibitors each week for 16days. Cell lysate was run by Western blot to detect LRRK2 and pS935 LRRK2, which is indicative of LRRK2 activity. Images shown are representative of =412 biological replicates. Quantification of Western blot of LRRK2 and pS935 LRRK2. LRRK2 levels were not significantly altered by one-way ANOVA, but pS935 levels were reduced to near undetectable levels (* <0.05, ** <0.01, Kruskal-Wallis test with Dunns multiple comparison test). Primary hippocampal neurons from CD1 pups were transduced with -synuclein PFFs and allowed to age a further 14days prior to fixation and staining for pS129 -synuclein (magenta), MAP2 (gray) and NeuN (blue). The neurons were additionally treated with LRRK2 inhibitors PF-475, PF-360, or MLi-2, 2days prior to transduction and fed with media containing inhibitors each week thereafter. No large differences can be observed in the type or abundance of -synuclein pathology. Quantification of -synuclein pathology reveals no change in response to LRRK2 inhibition, while PBS-treated neurons have no pathology (**** <0.0001 by Dunnetts multiple comparison test). MAP2 area ( ) and neuron number ( ) are also not altered in response to LRRK2 inhibition. No significant response was seen when compared with vehicle-treated neurons by one-way ANOVA with Dunnetts multiple comparison test ( ) or by Kruskal-Wallis test followed by Dunns multiple comparison test ( ) and ( ). ( =9 biological replicates). Means + s.e.m.; all values are normalized to neurons treated with -synuclein PFFs and DMSO. Scale bars=50m",yes
PMC10901953,Figure_8,oa_package/84/89/PMC10901953.tar.gz,"[' 8 and ', '']","Fig.8 Representative short-axis view cine magnetic resonance images. Control ( , ), 3days after the onset of myocardial infarction ( , ), and 9days after the onset of myocardial infarction ( , ). End-systolic phase of the rat heart ( , , ) and end-diastolic phase of the rat heart ( , , ). White dotted circles: infarcted area (cited from Tomography 2023, 9(2), 871882, Fig. )",yes
PMC4259387,Figure_2,oa_package/53/d2/PMC4259387.tar.gz,"['0114041-Torres1"" ref-type=""bibr"">[22] (see A), the monomeric and oligomeric Abeta were preferentially observed in membrane/plaque associated fractions, extracted with 2% SDS and 4% SDS/8M urea.', '0114041-Torres1"" ref-type=""bibr"">[22], in 6 12 months old PS1xAPP mice, these species were absent (or below the detection limit of our western blots) in the TBS soluble fraction or in the microsomal fraction (see A).', 'The lack of oligomeric Abeta in the TBS fraction was also probed by using a 6E10-6E10 oligomeric ELISA (AlphaLisa, Perkin Elmer) assay (B).', 'As shown, the TBS soluble fraction was contaminated with relatively high levels of monomeric Abeta (A).', 'Furthermore, we also observed the presence of different putative Abeta oligomers, such as dimers, trimers, and low molecular weight-Abeta in these contaminated soluble fractions (the presence of such oligomers was really high after 8 pulses of sonication, see A).', 'These Abeta oligomers were also clearly detected by 6E10-6E10 oligomeric Abeta ELISA (B).', 'g002The homogenization procedure has high impact on the integrity of Abeta plaques with the consequent solubilization and redistribution of Abeta in different fractions.', 'As shown in C, different amounts of Dounce-derived soluble fractions induced absolutely no microglial response, as determined by the expression of TNF-alpha, IL-6 and IL-1beta.', '0114041-Jimenez1"" ref-type=""bibr"">[9], the Dounce-derived TBS fraction from 12 month-old PS1xAPP produced no toxicity to N2a cells, whereas sonication-derived fractions produced a clear toxic effect (D).', 'However, sonication disrupted the Abeta plaques producing (after centrifugation) soluble monomers and, in minor extent, dimeric, trimeric and hexameric Abeta (see E, longer exposure).', 'This suggestion is based on: i) the soluble Abeta42 is detected predominantly in samples from demented individuals (Braak V VI), whereas similar Abeta40 levels are clearly observed in samples with few or no Abeta plaques (Braak II), moderate Abeta pathology (Braak III IV) and high pathology (Braak V VI); ii) as showed (see A), sonication produced a redistribution of Abeta from 2% SDS or 4% SDS/8M urea fractions (mostly Abeta plaques) to soluble fractions; iii) we observed a strong effect of sonication treatment on the integrity of biochemically isolated Abeta plaques.']",10.1371/journal.pone.0114041.g002,yes
PMC2941968,Figure_1,oa_package/d1/7d/PMC2941968.tar.gz,"['For example, when six practicing pathologists were asked to all photograph the same region on a glass slide with similar microscopes that had the same attached digital cameras, they all provided dissimilar images [].', '[3]Different digital images of the same region on a glass slide photographed at the same magnification by six different pathologists, each using similar microscopes and the same attached digital cameras (HER-2/neu immunohistochemical stain)WHOLE SLIDE IMAGINGWhole slide imaging (WSI), also referred to as virtual or wide-field microscopy, involves digitization of glass slides, which simulates light microscopy (i.']","Figure 1 Different digital images of the same region on a glass slide photographed at the same magnification by six different pathologists, each using similar microscopes and the same attached digital cameras (HER-2/neu immunohistochemical stain)",yes
PMC4028922,Figure_4,oa_package/15/c2/PMC4028922.tar.gz,"['As C-arm CT gives soft tissue information, the findings of C-arm CT and MDCT or magnetic resonance imaging (MRI) can be easily correlated and this helps both lesion targeting and follow-up comparison [].', 'C-arm CT detects lesions in difficult locations like segments 7 and 8 which can be obscured on DSA due to respiratory movement artifacts [].', 'Contrast-enhanced C-arm CT coronal MIP reformatted image shows the enhancement at recurrence site (arrowheads, B) and tumor feeder (arrow, B)(A-C)A 68-year-old male with segment 8 HCC.']","Figure 4(A-C) A 68-year-old male with segment 8 HCC. The lesion was not seen on DSA (A). Contrast-enhanced C-arm CT coronal MIP reformatted image shows the tumor (arrowheads, B) and tumor feeder (arrow, B). TACE was performed with lipidiol and adriamycin. One year follow-up CECT coronal reformatted image shows lipidiol uptake in lesion (arrowheads, C)",yes
PMC7412172,Figure_15,oa_package/40/e3/PMC7412172.tar.gz,[],"Figure 15 The schematic representation of the design and fabrication of the proposed microfluidic channel. Reprinted from Ref. [ ], Copyright 2019, with permission from Elsevier.",yes
PMC6006698,Figure_2,oa_package/f0/62/PMC6006698.tar.gz,"[' 2a).', 'TWF9 preferentially labels neurons in AD brain tissue.', 'neg ctrl negative controlTo determine if TWF9 immunoreactivity was specific to AD versus non-AD tissue, we performed fluorescent immunohistochemistry in AD tissue (n = 13), MCI (n = 8), and non-AD tissue cases (n = 7) (Table 1).', ' 2b, c).']","Fig. 2 TWF9 preferentially labels neurons in AD brain tissue. Alzheimers disease (AD) and mild cognitive impairment (MCI) cases stained with TWF9 (green) and counterstained with the nuclear marker Hoechst (blue). Immunostaining in AD and MCI tissue reveals TWF9 preferentially labels neurons. There are intercase differences in TWF9 immunoreactivity between AD and MCI cases. In AD#1 and MCI#1, TWF9 equally labels both the nucleus and cytoplasm in various neurons. TWF9 predominantly labels the nucleus in AD#3, and the cytoplasm in MCI#2 and AD#2. The scale bars located in the higher magnification inserts are 50m. Representative images of TWF9 immunostaining in AD, MCI, and nondemented age-matched controls. Quantification of fluorescent immunostaining with TWF9 in AD, MCI, and nondemented age-matched controls. Case breakdown is detailed in the Methods section. Data are expressed as meanSD. * <0.05, AD vs nondemented age-matched control by one-way ANOVA test with Tukeys post-hoc multiple comparison analysis. neg ctrl negative control",yes
PMC4055090,Figure_5,oa_package/5d/ca/PMC4055090.tar.gz,['sNEP- NSCs do not alter established tangle pathology but increase synaptic density and differentiate primarily into astrocytes.'],"Figure 5 Aged 3xTg-AD mice develop both A plaques and neurofibrillary tangles. We, therefore, examined whether sNEP-NSCs modulate tau pathology . Immunofluorescent labelling and quantification of phosphorylated tau (AT8 epitope: ser199/202) within the hippocampus revealed no significant differences between sNEP-NSC and control-NSC treated sides (n=5). This finding is in line with previous reports that decreasing A in aged 3xTg-AD mice does not reduce established insoluble tangle pathology. To determine whether sNEP expression leads to a functional effect on neuronal connectivity, we examined synaptophysin immunoreactivity. As shown, sNEP-NSCs increase synaptic density within the subiculum by more than 31% versus control-NSCs ( , =0.009, paired t-test). To examine the differentiation of transplanted NSCs, double-labelling for neuronal and glial markers was performed. In agreement with previous studies, very few NSCs (green) transplanted into the aged brain co-express the neuronal marker NeuN (blue, ). In contrast, the great majority of NSCs co-express the astrocytic marker GFAP (red, ). Scale Bar=100m in A-B, 15m in C, 6m in D-E, 15m in F, 300m in G-I, and 15m in J-L. A, beta amyloid; GFAP, glial fibrillary acidic protein; NSCs, neural stem cells; sNEP, secreted neprilysin.",yes
PMC10725466,Figure_3,oa_package/be/0c/PMC10725466.tar.gz,"[' 3A)73.', 'The metabolic engine of lymphatic endothelial cells.', 'Despite the well-established role of FAO in energy production in proliferative cells, pioneering work by Schoors et al.', ' 3A)64.', ' 3A).', ' 3B).', ' 3A).', ' 3A).', ' 3A).', ' 3A).', ' 3A).', ' 3A).', ' 3A, B).', ' 3A), controls LEC proliferation, migration, tube formation and invasion91.', ' 3A).', ' 3C).', ' 3C).']","Fig. 3 The metabolic engine of lymphatic endothelial cells. Central metabolism. Lymphatic metabolism is primarily driven by fatty acid (FA) metabolism (yellow) and the tricarboxylic (TCA) cycle, ketone bodies oxidation (KBO) (pink) and glucose metabolism (green). FAs are incorporated from the lymph fluid via CD36 and are transported into the mitochondria through CPT1A. Acetyl-CoA produced by FAO enters the TCA cycle to be oxidized. Acetyl-CoA can also be diverted towards FA synthesis. FAs can be stored in lipid droplets in the form of triacylglycerols (TAG) and can be released via lipophagy. Oxalacetate obtained in the TCA cycle, together with glutamate, can produce aspartate (Asp), which contributes to dNTP synthesis. In KBO pathway, -hydroxybutyrate (-OHB) produced by hepatocytes can be incorporated by LECs, rendering acetoacetyl-CoA upon the consecutive reactions catalyzed by BDH1 and OXCT1 enzymes. In the mitochondria, acetoacetyl-CoA is converted into acetyl-CoA, which enters the TCA cycle. By producing acetyl-CoA, FAO and KBO support the TCA cycle and thereby contribute to dNTP synthesis and ATP production via oxidative phosphorylation (OXPHOS). Acetyl-CoA can be also transported to the cytosol through the SLC25A1 transporter, and then it can enter the nucleus. Moreover, malonyl-CoA produced during FA synthesis can be imported into the nucleus in the form of acetyl-CoA after decarboxylation by the malonyl-CoA decarboxylase (MCD). The complex III of the mitochondrial respiratory chain regulates the PROX1-VEGFR-3 feedback loop. Another important metabolic pathway in LECs is glycolysis. LECs incorporate glucose (Glc) from the lymph fluid and exhibit high glycolytic rates that contribute to ATP production, amino acid synthesis and pentose phosphate pathway (PPP). Moreover, upon interaction with FGFR-1/3, FGF-2 upregulates hexokinase-2 (HK2; rate-limiting enzyme of glycolysis) through the c-MYC transcription factor. Transcriptional regulation of lymphatic metabolism. By acting as a p300 substrate, acetyl-CoA produced by FAO, in conjunction with PROX1, promotes lymphatic gene expression (VEGFR-3, PROX1 and CPT1A). Moreover, upon FGF/FGFR axis blockade, the impairment of the ERK signaling pathway triggers increased CPT1A expression through the activation of the nuclear receptor PPAR-, finally promoting CPT1A transcription to stimulate FAO as a compensatory mechanism. Amino acid metabolism. Tryptophan (Trp) metabolism involves the conversion of Trp to L-kyrunenine by indoleamine 2,3-dioxygenase (IDO1). L-kyrunenine can then be converted to 3-hydroxyl-L-kyrunenine, which can ultimately generate quinolinic acid (canonical pathway) or it can render the biogenic amine 3-hydroxy-L-kynurenamine (3-HKA). 3-HKA exerts an anti-inflammatory role by the activation of pro-inflammatory pathways within dendritic cells (DC). Cystathionine--synthase (CBS) modulates lymphatic function through the induction of VEGFR-2/3 and the hydrogen sulfide (H2S) production during amino acids transulfurylation. This process involves the generation of cystathionine, H S and water from methionine (Met)-derived homocysteine, cysteine (Cys) and serine (Ser). Further metabolization of cystathionine forms cysteine (Cys) and glutamate (Glu). ACC acetyl-CoA carboxylase, Asp aspartate AST aspartate aminotransferase, -OHB -hydroxybutyrate BDH1 D--hydroxybutyrate dehydrogenase, CBS cystathionine--synthase, Cys cysteine, DCs dendritic cells, FAO fatty acid oxidation, FAs fatty acids, FASN fatty acid synthase, G6P glucose-6-phosphate, Glu glutamate, GLUT glucose transporter, H S hydrogen sulfide, HK2 hekoxinase-2, IDO1 indoleamine 2,3-dioxygenase, KBO ketone body oxidation, Lac lactate, LDH lactate dehydrogenase, MCD malonyl-CoA decarboxylase, MCT1/2 proton-linked monocarboxylate transporter, Met methionine, OAA oxalacetate, OXCT1 3-oxoacid CoA-transferase 1, OXPHOS oxidative phosphorylation, PDH pyruvate dehydrogenase, PPP pentose phosphate pathway, Pyr pyruvate, Ser serine, TAG triacylglycerols, TCA tricarboxylic acid, Trp tryptophan. The asterisks (*) indicate the unique molecular and metabolic peculiarities of the lymphatic endothelium. Figure was created with Adobe Illustrator and the DNA strand was created with BioRender.com.",yes
PMC9793177,Figure_4,oa_package/fb/18/PMC9793177.tar.gz,['The patient underwent four cycles of Pemetrexed-Cisplatin protocol according to the biopsy result.'],"Fig. 4 Histopathology from biopsy (A) with 40 magnification showed tumor growth in a glandular pattern, which consists of the proliferation of anaplastic cells with round-oval, pleomorphic, and rough chromatin nuclei, consistent with adenocarcinoma. IHC smear (20 magnification) with Napshin-A positive within the tumor cell cytoplasm (B) and WT-1 negative in tumor cell nuclei (C), suggesting tumor origin from the lung.",yes
PMC10400774,Figure_7,oa_package/de/68/PMC10400774.tar.gz,"['The immunohistochemical staining pattern of activated complement deposition also differs: a rosette-like staining around blood vessels is seen in AQP4 + NMOSD (49, 87, 88), while in MOGAD, perivascular complement deposition is much less () but stained on myelinated fibers and in myelin degradation products within macrophages (; Tables 2, 3) (37, 38, 40).', 'Comparison of the deposition pattern of activated complements in MOGAD and AQP4 + NMOSD.']","Figure 7 Comparison of the deposition pattern of activated complements in MOGAD and AQP4+NMOSD. Only mild perivascular depositions of complements were seen in MOGAD even in the active lesion where perivascular cuffing was evident. In active demyelinating lesions, complement staining was detected on myelin debris phagocytosed by macrophages (red arrow). Multiple rosette-like stainings of complement deposition were seen in the NMOSD lesion. Perivascular complement depositions were seen within AQP4-loss lesions. C9neo. MBP/CD68, AQP4. AQP4, aquaporin 4; MBP, myelin basic protein; MOGAD, myelin oligodendrocyte glycoprotein antibody-associated disease; NMOSD, neuromyelitis optica spectrum disorders.",yes
PMC3369494,Figure_5,oa_package/e3/47/PMC3369494.tar.gz,"['The patient was followed up 3, 6, 12 and 24 months after the prosthetic rehabilitation (s 5 and 6).', '004""/>Radiological view after prosthetic rehabilitation.']",Figure 5 Radiological view after prosthetic rehabilitation.,yes
PMC2765174,Figure_10,oa_package/3f/36/PMC2765174.tar.gz,[],"Figure 10 (A,B) Axial T2W image (A) demonstrates a post-lumpectomy seroma cavity (arrow) in this patient with positive margins after conservation therapy for breast cancer. The axial DCE-MRI subtracted image demonstrates minimal thick, nodular enhancement in the posteromedial and lateral walls of the lumpectomy cavity (arrows). In this patient, re-excision of the cavity with attention to the areas noted on the DCE-MRI yielded negative margins",yes
PMC10583284,Figure_6,oa_package/75/a4/PMC10583284.tar.gz,"['.', 'Our analyses demonstrate that while wild-type and S15Y variant signal peptides successfully dock in the SRP54 hydrophobic pocket crucial for signal peptide recognition, W8R does not (B D, right panels).']","Figure 6. molecular modeling of SRP54 interactions with signal peptides. ( ) Positions of mutations W8R (PPV, marked in red) and S15Y (NPV, marked in black) in the signal peptide of ALK receptor tyrosine kinase. Signal peptide was predicted by SignalP6.0. (BD) Modeling of the wild-type ALK signal peptide ( ) and the mutants, S15Y ( ), W8R ( ). The hydrophobicity of each signal peptide was determined using the Kyte-Doolittle scale and shown in the left panels. Signal peptide mutants (red line) are compared to the WT ALK signal peptide (black line) to determine the predicted change in hydrophobicity. The top 100 (pink) and top 1000 (blue) models of the corresponding signal peptides and SRP are created using ROSIE (Rosetta Online Serve that Includes Everyone) and their distribution is shown in the central panels. The top models, which are most minimized, are labeled. These top models were selected and visualized by the use of PyMol and shown in the right panels. Wild-type signal peptide (WT) is shown in green, mutated signal peptides are in orange, and M-domain of SRP54, a subunit of SRP, is shown in light blue.",yes
PMC5033939,Figure_2,oa_package/f9/d8/PMC5033939.tar.gz,"['Initial biodistribution studies using Evans blue dye illustrated that intravenous injection via the facial vein led to superior systemic distribution compared with intraperitoneal injection (Supplemental ); intravenous injection was therefore utilized for subsequent gene therapy delivery.', 'Intravenous injection of AAV9-UBA1 at P1 into control healthy mice led to significant increases in UBA1 protein levels in the spinal cord, muscle, heart, liver, lung, and kidney by P7 (, A and B).', 'qRT-PCR analysis using primers designed to specifically detect human or mouse UBA1 revealed that the UBA1 increase seen in tissue from AAV9-UBA1 injected mice was exclusively attributable to virally delivered human UBA1 cDNA expression, as mouse Uba1 mRNA levels remained unchanged (, C and D).', 'Neuropathological analyses of AAV9-UBA1 treated control mice at P9 showed no detectable changes in spinal motor neuron number or morphology (, E and F), neuromuscular junction (NMJ) integrity (, G and H, and Supplemental , A and C), or muscle fiber diameter (Supplemental , B and D).', 'In a longer-term safety study, the appearance (I), weight (J), survival (K), and motor performance (L) of AAV9-UBA1 mice remained indistinguishable from uninjected control mice throughout (endpoint of P180).', 'As the heart had the highest fold changes in UBA1 protein levels following gene therapy delivery (, A and B, and , B and C), we chose to conduct these investigations in heart tissue from control and SMA mice.', 'As intravenous injections were found to be superior at achieving systemic distribution, all subsequent delivery of gene therapy was given via injection into the facial vein in TTG or CD1 wild-type mice, conducted under chilled anesthesia using a 33-gauge needle and Hamilton syringe (Supplemental ).', 'Visualization of the facial vein for intravenous injection was aided by using a Wee Sight Transilluminator LED light (Philips) (Supplemental ).', 'AAV9-UBA1 gene therapy delivers safe systemic increases in UBA1 expression.', 'As part of a different comparative analysis, weight, survival and righting times (A C) for uninjected controls are also shown in , J L.', 'As part of a different comparative analysis, uninjected littermate control data motor neuron cell body counts (B) and NMJ axonal input (D) is also shown in , F and H, respectively.']","Figure 2 AAV9-UBA1 gene therapy delivers safe systemic increases in UBA1 expression. ( and ) Adeno-associated virus serotype 9ubiquitin-like modifier activating enzyme 1 (AAV9-UBA1) delivered at P1 significantly increased UBA1 protein levels in spinal cord, gastrocnemius muscle, heart, liver, lung, and kidney in P7 control mice ( = 3 mice per group; unpaired 2-tailed Students test). Lanes were run on the same gel but were noncontiguous. ( ) PCR products following mouse, human, and UBA1 viral cDNA amplification using primers detecting both mouse and human cDNA (top row), unique to mouse cDNA (middle row), and unique to human cDNA (bottom row). ( ) Significant increase in AAV-expressed human mRNA, but not endogenous mouse mRNA, in the hearts of P7 AAV9-UBA1treated mice ( = 3 mice per group; unpaired, 2-tailed Students test). ( ) Representative Nissl-stained spinal cords (ventral horn) from uninjected control and AAV9-UBA1treated control mice at P9 (scale bar: 250 m). ( ) No significant change in the number of motor neurons in the spinal cords of AAV9-UBA1treated control P9 mice ( = 4 mice per group; unpaired, 2-tailed Students test). ( ) Representative confocal micrographs of neuromuscular junctions (NMJs) in the external oblique from uninjected control and AAV9-UBA1treated control mice at P9 (axonal inputs in green; postsynaptic endplates in red; scale bar: 25 m). ( ) No significant change in axonal inputs at the NMJ in the external oblique muscle of AAV9-UBA1treated control mice at P9 ( = 4 mice per group; unpaired, 2-tailed Students test). ( ) Uninjected control and AAV9-UBA1treated control mice at P180 (scale bar: 2 cm). ( ) No change in AAV9-UBA1treated controls from P1 to P180 with respect to ( ) weight (at P180 = 4 mice per group), ( survival (Kaplan-Meier survival analysis), or ( ) righting reflex test performance (from P1 to P12P14; unpaired, 2-tailed Students test at each time point). ns (not significant) > 0.05 * 0.05, ** 0.01, *** 0.005, **** 0.001. As part of a different comparative analysis, uninjected littermate control data concerning motor neuron cell body counts ( ) and NMJ axonal input ( ) is also shown in , respectively. Weight, survival and righting times ( ) for uninjected controls are also shown in .",yes
PMC7563035,Figure_3,oa_package/7d/29/PMC7563035.tar.gz,['Immunohistochemical assay for a) aggrecan (AGG) and b) collagen-II (Col-II) in each group.'],"Fig. 3 Immunohistochemical assay for a) aggrecan (AGG) and b) collagen-II (Col-II) in each group. c) Intensity of optical density (IOD) values of AGG in different groups. d) IOD values of Col-II in different groups. Data are presented as the mean (SD), all p-values were measured using one-way analysis of variance (ANOVA). Thin bars = 100 m. Thick bars = 50 m. OA, osteoarthritis; PTH, parathyroid hormone.",yes
PMC9621137,Figure_2,oa_package/a1/37/PMC9621137.tar.gz,"['A83G splice donor site mutation resulted in complete skipping of exon 2, effectively removing 106 bp including the start codon from the mRNA transcript, by which amino acids 1 67 of METTL23 would not be translated (, A and B).', 'A83G mutation and maintained pluripotency as evidenced by the expression of typical pluripotent stem cell markers (, C and D, and Supplemental ).', 'A83G iPSCs by TA cloning following Sanger sequencing (E).', 'The first skipped exons 2 and 3, while the other skipped exon 2 and inserted the initial 35 bp of intron 1 (, E G).', '84+60delAT) was found in 14 individuals, reported to be unrelated, from a collection of 1,029 Japanese NTG cases and in 8 of 1,402 age-matched Japanese controls (Table 2, B, and Supplemental Tables 5 and 6).', 'The METTL23 c.']","Figure 2 The c.A83G mutation leads to splicing in transfected HEK293T cells and iPSCs. ( ) The splicing construct minigene was generated by incorporating the genomic region of the gene into the pSpliceExpress vector via XhoI and XbaI restriction sites. Vector exons are depicted as black boxes, and the exon 2 is shown as a gray box. The locations of the mutations are marked by arrowheads (red: c.A83G; yellow: c.84+60delAT). ( ) Gel electrophoresis of RT-PCR products from transfected HEK293T cells. The primers are indicated by arrows in . EV, empty vector; PBS, cells transfected with PBS only; PCR-n, PCR negative control. WT and mutant transcript contents were determined by Sanger sequencing and are depicted to the right of the gel image. ( ) Graphic display of iPSC preparation. Collected samples are marked with a red box in A. Scale bar: 200 m. ( ) Characterization of iPSCs. A83G-iPSC colonies (glaucoma a and b) were derived from lymphocytes from patients with NTG. There was no difference between iPSC controls (normal a/b) and A83G-iPSCs (glaucoma a/b) during induction and cultivation. Scale bar: 200 m. ( ) Gel electrophoresis of RT-PCR products from iPSCs. TA-cloning analysis following Sanger sequencing identified 4 splicing variants in A83G-iPSCs (glaucoma a/b). ( ) Schematic representation of the splicing variants. ( ) Predicted protein structures of the splicing variants (red: p.E28G). The 2 predicted proteins lack motif 1 and motif post 1, suggesting that the mutant alleles are functionally null. C-term, C-terminal; N-term, N-terminal.",yes
PMC9790902,Figure_3,oa_package/b5/a3/PMC9790902.tar.gz,"['As expected, commercial PVA bead (100 300 m) is exclusively observed within the scope of VX2 carcinoma, 24 h after embolotherapy (A).', 'Although its is size only around 1 m, at both time points (1 h in B, 24 h in C) we have not observed it overflow from the VX2 carcinoma into the adjacent liver lobe, as LPM-10 did.']",FIGURE 3 Representative slices of commercial bead trapped VX2 carcinoma. Representative slices of sevelamer trapped VX2 carcinoma and their local magnifications 1 and 24h after the procedure. Some sevelamer particles were aggregated within the big or small vessels due to their deformability. The vessels with a size around or below 40m are also found to be fully embolized by the aggregates . Hematoxylin-eosin staining of pure sevelamer was set as control .,yes
PMC11419762,Figure_3,oa_package/ff/af/PMC11419762.tar.gz,"['51\n52\n53\n54\n55\n\nAmong the central nervous system (CNS) complications, the most frequently reported adverse event is cerebral venous sinus thrombosis, with superior sagittal and sigmoid sinus being the most commonly thrombosed sinuses seen on MR or CT venography (\n\n).', '41\n42\n58\n59\n60\n61\n62\n63\n64\n65\n66\n67\n68\n\nA 33-year-old woman presented with severe headache 3 days after the first dose of ChAdOx1 vaccination.']",Fig. 3 A 33-year-old woman presented with severe headache 3 days after the first dose of ChAdOx1 vaccination. There was hemorrhagic infarct in the left thalamus extending to the midbrain (not shown). Magnetic resonance (MR) venography revealed nonvisualization of the left sigmoid sinus ( ) indicating venous thrombosis. The right sigmoid sinus was normally seen ( ).,yes
PMC6783265,Figure_13,oa_package/3b/a2/PMC6783265.tar.gz,[],10.7554/eLife.48378.016,yes
PMC10398960,Figure_1,oa_package/e4/0a/PMC10398960.tar.gz,"['Using an upright light microscope and an AFM positioned on a motorized XY-stage (A), we generated high resolution (30 m steps) mechanical maps of freshly cut medial hippocampal slices of 500 m thickness.', 'Mechanical maps (1,500 450 m) that spanned ten clearly identifiable subregions of the hippocampus (B) were generated for comparisons between age and genotype.', ', the pyramidal and granule cell bodies) were labeled as regions 2, 6, and 9 (B) and were analyzed separately to the molecular layer of the dentate gyrus (region 5) and the stratum lacunosum-moleculare (region 4).', '0001) (F).', '0001) (F).', 'Interestingly, this decrease in GFAP fluorescence intensity coincided with an increase in tissue stiffness (F).', '(B) GFAP fluorescence intensity was quantified in the ten hippocampal subregions and grouped as described in B.', 'The significant differences found in F and panel (B) have been highlighted with directional arrows for clarity.', 'Ten distinct hippocampal cell layers were chosen for analysis (B).']","FIGURE 1 Hippocampal CA1 and dentate gyrus soften in aged mice with Alzheimers disease like amyloid pathology. Schematic of the combined AFM, upright camera, and perfusion chamber set-up used to measure the microscale stiffness of acute hippocampal slices. Cross section of the medial hippocampus showing the 10 subregions and the locations from which stiffness maps were extracted. Representative hippocampal stiffness maps of 3-month wild-type in , 18-month wild-type in panel and 17-month APP in panel . The elastic moduli of individual indentation points are color-coded, with dark red colors representing softer areas and bright yellow colors depicting stiffer tissue regions. Scale bars = 450 m. Stiffness of the hippocampal substructures depicted in panel . The stratum oriens/radiatum (SO/SR) of the CA1 are grouped and represented as regions 1 and 3, respectively. The Hilus, CA3 stratum oriens and CA3 stratum radiatum are also grouped and represented by the numbers 7, 8, and 10, respectively. The stratum lacunosum-moleculare (SLM) is represented by 4, and the molecular layer (ML) of the dentate gyrus is represented by 5. Finally, the three main neuronal cell body regions of the hippocampus, i.e., the granule cells of the dentate gyrus (DG), and the pyramidal cells of the CA3 and CA1 regions, are represented by the numbers 6, 9, and 2, respectively. AFM experiments were conducted on hippocampal slices from 3-month wild-type ( = 12), 18-month wild-type ( = 11), and 17-month APP mice ( = 4). The vertical bars represent the means, the horizontal lines represent the medians, and the whiskers are the standard deviations of all experimental data points. Gray dots represent the individual data points for each AFM measurement. The black dots represent the average of the AFM measurements for each animal. X indicates the absence of some data points larger than 800 Pa (outliers were less than 0.5% of all data points). *** < 0.001.",yes
PMC9763373,Figure_1,oa_package/75/ae/PMC9763373.tar.gz,[],Image 1 Axial (left) and coronal (right) CT scan - sigmoid mass (black arrow).,yes
PMC9457692,Figure_3,oa_package/9f/74/PMC9457692.tar.gz,"[' -Amyloid (1-42) Immunostaining in the Hippocampal Dentate Gyrus of SAMP8 MiceThe intraneuronal accumulation of -Amyloid (1-42) peptides was also observed in the hippocampal dentate gyrus of control mice, specifically in the granule neurons layer and hilar neurons (a,c).', 'However, the levels of -Amyloid (1-42) decreased in both types of neurons in the melatonin-treated mice (b,d).', 'We observed statistically significant differences in the levels of -Amyloid (1-42) peptides between control mice and melatonin-treated mice (e, p 0.', ' -Amyloid (1-42) immunostaining in the hippocampal dentate gyrus of SAMP8 control mice (a,c) and SAMP8 mice treated with melatonin (b,d).']","Figure 3 -Amyloid (1-42) immunostaining in the hippocampal dentate gyrus of SAMP8 control mice ( , ) and SAMP8 mice treated with melatonin ( , ). Bar chart ( ) shows quantification of the DAB signal with respect to the total number of nuclei at 400 magnifications (in percentages with respect to the control). Data are expressed as means SEM. ** < 0.01 vs. control. ML, the molecular layer; GNL, the granule neurons layer; SGZ, the subgranular zone. Statistical analysis was always performed in 10 images obtained from each control mice ( = 4) and mice treated with melatonin ( = 4).",yes
PMC8640699,Figure_3,oa_package/10/38/PMC8640699.tar.gz,"['2ALP (U/L)240236234 217 187SGPT (U/L)319224132 67 69SGOT (U/L)275254169 52 54Chest X-ray PA viewFew patchy areas of ground-glass opacities in B/L lungs; mild B/L pleural effusions (black arrow); calcified mediastinal and hilar lymphadenopathy (white arrow)PA: posteroanterior; B/L: bilateralAfter the initial evaluation, he was put on an oral steroid, antihistaminics, topical oral antifungal suspension, and artificial tears.']",Figure 3 Chest X-ray PA view Few patchy areas of ground-glass opacities in B/L lungs; mild B/L pleural effusions (black arrow); calcified mediastinal and hilar lymphadenopathy (white arrow) PA: posteroanterior; B/L: bilateral,yes
PMC10638434,Figure_4,oa_package/18/b2/PMC10638434.tar.gz,"[' 4a.', ' 4b).', ' 4b).', ' 4b).', 'Amyloid pathology in brain of 12-month-old APPNL-G-F/NL-G-F mice.', 'We also investigated the deposition of A plaques in various brain regions such as mPFC, HPC, RSA, PRhC, and CAA as these brain regions are involved in various cognitive functions including learning and memory (', ' 4ci v).', ' 4cii), RSA (P 0.', ' 4ciii), PRhC (P 0.', ' 4civ) and CAA (P 0.', ' 4cv) when compared to APP-NT mice.', ' 4ci v).', ' 4ci).', ' 4d.', ' 4e).', ' 4f).']","Fig. 4 Amyloid pathology in brain of 12-month-old APP mice. Photomicrographs of amyloid plaques stained with methoxy-XO (green) in two brain sections (top: bregma +1.94mm; bottom: 3.08mm). Summary results of amyloid plaque area in the brain. Amyloid plaques area in medial prefrontal cortex (mPFC)(i), hippocampus (HPC)(ii) retrospenial area (RSA)(iii), perirhinal cortex (PRhC)(iv), and cortical amygdalar area (CAA)(v). Scale bars represent 1mm for section +1.94mm and 2.5mm for section 3.08mm. Photomicrographs of amyloid plaques stained with methoxy-XO (green) in MSDB complex. Total amyloid plaque number in MSDB complex. Amyloid plaque area in MSDB complex. Scale bar500m for whole sections. Data is presented as meanSEM. * <0.05, ** <0.01, ** <0.001; as compared to APP-NT group. as compared to the APP-ST group. APP-NT group ( =4)APP with no training. APP-ST group ( =3)APP mice exposed to single-domain cognitive training (ST). APP-MT group ( =3)APP mice exposed to multi-domain cognitive training (MT).",yes
PMC7573563,Figure_1,oa_package/e1/f0/PMC7573563.tar.gz,"[' summarizes the effects of DENV infection during ADE and non-ADE conditions.', 'Innate immune response during ADE and non-ADE dengue infection.']","Figure 1 Innate immune response during ADE and non-ADE dengue infection. Canonical non-ADE mediated entry occurs via receptor-mediated endocytosis. Upon entry, the DENV particles are internalized in endosomes and are recognized by the pathogen recognition receptors TLR-3 and 7. Release of viral RNA from endosomes is recognized by RIGI and MDA5 which triggers production of pro-inflammatory cytokines IFN- and IL-8. This activates the JAK/STAT pathway resulting in expression of IFN-, IL-12, and Nitric Oxide radicals. Virus entry via FcR-antibody in dengue-ADE caused expression of TANK and SARM which inhibits TLR signaling. Production of anti-inflammatory cytokines IL-10 and IL-6 ensues and expression of SOCS3 as a result inhibits JAK/STAT pathway. This results in inhibition of pro-inflammatory cytokine production and causing a TH-2 biased immune response and increased burst size.",yes
PMC4520273,Figure_2,oa_package/44/5e/PMC4520273.tar.gz,"[' 2a) revealed the presence of inclusions that were immunoreactive for antibodies to S (Syn506), Ser129 phosphorylated S (pSer129/81A) and a general inclusion marker (p62) in mice injected with fibrils produced from S or cation exchanged S.', ' 2b) showed a high concentration of inclusions at the site of injection in both sets of mice and quantification of Syn506 staining in this region revealed no difference between the two groups (', ' 2c).', ' S pathology in M83 S Tg mice following injection of S or cation exchanged S fibrils.', 'Error bars represent the standard error of the meanImmunofluorescence analysis of S pathology in M83 S Tg mice following injection of S fibrils.']","Fig. 2 S pathology in M83 S Tg mice following injection of S or cation exchanged S fibrils. Representative immunohistochemical images of cortical, hippocampal, brainstem, and spinal cord sections of hemizygous M83 S Tg mice stereotaxically injected in the hippocampus with S or LPS purified (cation exchanged) S. Antibodies to S (Syn506), to S phosphorylated at Ser129 (pSer129/81A), and a general inclusion marker (p62) were used. Scale bar=50m. Schematic representation of the distribution of S inclusion pathology in the neuroaxis of hemizygous M83 S Tg mice injected with S or cation exchanged S fibrils. Red dots indicate the sites and amounts of S pathology. Blue arrows indicate the site of injection. Quantification of Syn506 staining in the hippocampus of mice injected with S or cation exchanged S fibrils. The p-value was calculated using a two-tailed -test. Error bars represent the standard error of the mean",yes
PMC11520109,Figure_4,oa_package/bf/17/PMC11520109.tar.gz,['(i-k) The appearance of functional recovery of the affected limb was evaluated as good 1 year after surgery\n\nA 28-year-old male with left RHF and CCI.'],"Fig. 4 A 65-year-old female with left RHF and CCI. ( , ) Preoperative X-ray radiographs showed RHF. ( ) Preoperative CT showed Mason Type II RHF. ( , ) Preoperative MRI showed RHF with CCI. ( ) Intraoperative exploration showed RHF with Type III CCI (blue arrow). ( , ) X-ray radiographs at 3 months after surgery showed fracture healing. ( - ) The appearance of functional recovery of the affected limb was evaluated as good 1 year after surgery",yes
PMC9204035,Figure_3,oa_package/08/87/PMC9204035.tar.gz,"['In , the oxygen supply mask (indicated by a white arrow in the image) was cropped to produce a clearer lung field.', 'Comparison of images before and after manual cropping.']",Figure 3 Comparison of images before and after manual cropping.,yes
PMC10226183,Figure_4,oa_package/27/79/PMC10226183.tar.gz,[],Fig. (4) DOS inhibits intestinal UA reabsorption. ( ) Representative photomicrograph of GLUT9 protein expressions in duodenum and ileum tissues by IHC staining observed at 400. ( & ) Semi-quantitative analysis of GLUT9 protein expressions in duodenum and ileum presented as IOD/Area. IOD-integrated optical density; NC-normal control group; MC-model control group; DOS-H-high dose DOS group; DOS-M-middle dose DOS group; DOS-L-low dose DOS group. The data are expressed as mean SD. <0.05; <0.01 the NC group; * <0.05; ** <0.01 . the MC group.,yes
PMC3868479,Figure_3,oa_package/5f/be/PMC3868479.tar.gz,"['g003Bacterial invasion in shed urothelial cells.', '(C) A Z-axis profile plot was produced\nas in .']",10.1371/journal.pone.0083637.g003,yes
PMC5748183,Figure_4,oa_package/62/70/PMC5748183.tar.gz,[],"Fig. 4. Inhibition of calcineurin and FKBP12 by low doses of Tacrolimus protects against -syn toxicity in vivo. ( ) Diagram representing the rat in vivo experiment; briefly, = 2530 rats were injected unilaterally in the SNc with an AAV1/2 carrying either A53T -syn or empty vector (CT). Four days after injection, rats were treated with different s.c. amounts of Tacrolimus on a weekly basis for 6 wk. Before the animals were killed, ( ) the behavioral test for paw asymmetry was performed. Subsequently, striatal neurochemistry was performed. * < 0.02 (one-way ANOVA, Dunnetts multiple comparison test); ** < 0.007 (one-way ANOVA, Dunnetts multiple comparison test). ( ) DAT assayed by autoradiography at the striatum and normalized to its non-syninjected contralateral side. * < 0.03 (one-way ANOVA, Dunnetts multiple comparison test); **** < 0.0001 (one-way ANOVA, Dunnetts multiple comparison test). ( ) DA measured by HPLC. ** < 0.0016 (one-way ANOVA, Dunnetts multiple comparison test); **** < 0.0001 (one-way ANOVA, Dunnetts multiple comparison test). ( ) Striatal samples from CT, -syn, and -syn with <5 ng/mL of Tacrolimus were subjected to TMT MS ( ), and phosphopeptides that were significantly rescued by Tacrolimus are shown. These phosphosites belong to two proteins: GAP-43 and BASP1. The phosphorylation site identified is highlighted in red. = 3 rats. * < 0.05 (two-tailed test).",yes
PMC5306191,Figure_5,oa_package/4c/fd/PMC5306191.tar.gz,"[' 5).', '', 'Reprinted with permission from the American Association for the Advancement of Science (AAAS)\nWhether there are differences in the glycosylation of the MUC2 secreted at the surfaces of the crypts, from either the sentinel cell or those neighbouring goblet cells, has not yet been examined.']",Fig.5 Sentinel goblet cells in the human colon. Goblet cells responsive to Toll-like receptor ligands (TLR ligands) are located in the upper crypt. Cryosections in colonic explants treated with TLR ligands and visualized by confocal microscopy. MUC2; DNA. Upper crypt ( ) or lower crypt ( ). A shows the epithelial surface. 20mm From Birchenough et al. Science 352:15351542 ( ). Reprinted with permission from the American Association for the Advancement of Science (AAAS),yes
PMC2954927,Figure_1,oa_package/f9/11/PMC2954927.tar.gz,['Medial aspect of the knee.'],"Figure 1 . (A). Photograph showing the medial side of the right cadaveric knee joint. (B). the distances from the centroid of femoral insertion and tibial insertion to the joint line. sMCL = superficial medial collateral ligament, dMCL = deep medial collateral ligament, POL = posterior oblique ligament. ME = medial epicondyle, MGT = medial gastrocnemius tubercle, MAT = medial adduct tubercle.",yes
PMC7718647,Figure_3,oa_package/2e/58/PMC7718647.tar.gz,"['001, a and b); meanwhile, there were no significant differences between the NC and pcDNA 3.', 'The cell apoptosis rate of difference groups by flow cytometry in HeLa and SiHa cell lines.']","Figure 3 The cell apoptosis rate of difference groups by flow cytometry in HeLa and SiHa cell lines. NC: normal control group; pcDNA 3.1: the cells were transfected with pcDNA3.1; lncRNA: the cells were transfected with UBE2R2-AS1 by pcDNA3.1. (a) The cell apoptosis rate of difference groups by flow cytometry in HeLa cell. *** < 0.001, compared with NC group. (b) The cell apoptosis rate of difference groups by flow cytometry in SiHa cell. *** < 0.001, compared with NC group.",yes
PMC4367107,Figure_2,oa_package/55/a8/PMC4367107.tar.gz,['.'],Fig. 2. Immunohistochemical expression of wild-type HSP110 (HSP110wt) and thymidylate synthase (TS) in microsatellite instabilityhigh colorectal cancers. (A) A case showing high-level expression of HSP110wt (score 3+). (B) A case showing HSP110wt-negativity (score 0). (C) A case showing high-level expression of TS (score 3+). (D) A case showing low-level expression of TS (score 0).,yes
PMC4819645,Figure_3,oa_package/87/5a/PMC4819645.tar.gz,"['MRI scan findings of high signal of, microtearing of,33 or separation of the PA from the pubic bone (figure 3).']","Figure3 Fat suppressed sagittal view of the groin. SCF, subcutaneous fat; RA, rectus abdominis; P, pubic bone; ALT, adductor longus tendon; PAD, pubic aponeurosis defect.",yes
PMC11635299,Figure_5,oa_package/48/18/PMC11635299.tar.gz,"['Additionally, the embryos displayed hemorrhagic lesions and enlarged livers that exhibited either yellow to reddish foci or discoloration ().', 'Gross lesion in the embryos, CAM, liver, heart, and kidney.']","Figure 5 Gross lesion in the embryos, CAM, liver, heart, and kidney. Normal embryo (black arrow), accumulation of edematous fluid in the IBHV infected embryos (black arrow), development of pock lesion on the CAM of IBHV infected embryos (black arrow), normal structure of the liver of control embryos (black arrow), white nodular lesion on the liver of virus infected embryos (black arrow) and accumulation of excess fluid in the pericardium of the virus infected embryos (upper black arrow), and development of white nodular mass in the kidney of virus infected embryos (black arrow).",yes
PMC7394155,Figure_2,oa_package/a7/50/PMC7394155.tar.gz,"['HE 200 , bar = 50 mFemale rat administered a high-dose acitretin solution.']","Figure 2 Female rat administered a high-dose acitretin solution. HE 100, bar = 100 m",yes
PMC6246744,Figure_3,oa_package/8e/de/PMC6246744.tar.gz,[],"FIGURE 3 Soluble fractions (S1) from aged ThyTau22 mice were toxic for BV2 microglial cells whereas S1 from aged P301S mice had no effect in the viability of microglial cells. Representative Annexin V/propidium iodide (PI) double staining of BV2 cells treated with S1 fractions at 0.1 ug/ul for 12 h. Quantitative analysis of cell viability after exposure to S1 fractions. Data is shown as the mean + SD ( = 35). < 0.05. Significance was determined by ANOVA and Tukey post-hoc test. Caspase activation following microglial treatment with soluble phospho-tau from 12 month-old ThyTau22 mice. Representative western blots showing caspase 3, 8, and 9 activation in BV2 cells treated with S1 from aged ThyTau22 mice (0.1 ug/ul). B-actin (low panel) was used as the loading control. The relative abundance of cleaved caspases was determined by densitometry analysis. Data were normalized by positive control (staurosporine 1 M), and significance was determined by ANOVA and Tukey post-hoc test ( = 3). No differences in ATP levels were observed in BV2 cells treated with S1 fractions from 12 month-old ThyTau22 mice (0.1 ug/ul). Data is shown as the mean + SD ( = 3).",yes
PMC7425448,Figure_2,oa_package/ac/79/PMC7425448.tar.gz,"['To explore whether Mino exerted a protective role on the dopaminergic neurons, we quantified the TH+ cells bodies in the SN and striatal fibers after Mino treatment (A, 2B).', 'Mino administration after AAV injection prevented the loss of dopaminergic cell bodies in the substantia nigra (SN) and striatal fibers.']","Figure 2 ( ) Immunohistochemical staining of the tyrosine hydroxylase (TH) cells in the striatum and SN. ( , ) Quantification of TH cells in SN and fibers in striatum. Data are expressed as the mean standard deviation (SD). * < 0.05; ** < 0.01.",yes
PMC10449997,Figure_8,oa_package/8f/66/PMC10449997.tar.gz,"[' 8a).', ' 8g).', ' 8h).', '0001In line with our previous findings [19], cells transfected with TRPC6 G757D/wild-type mixture showed reduced responses to the carbachol (', ' 8b) and DOG (', ' 8e), while their results are comparable with G757D alone.', ' 8g, h), thereby suggesting that the LOF response is lower than the TRPC6 wild-type response; however, such a low response is not sufficient to make an impact on the functionality of the channel in comparison to GOF phenotype showing a higher response.', ' 8c, g) and DOG (', ' 8f, h).']","Fig. 8 Co-transfection strategy to characterize the dominant phenotype of TRPC6 mutants. Changes in intracellular calcium concentration ( / ) were measured in Fluo-4-AM-loaded HeLa cells expressing wild-type (WT), P112Q/WT, G757D/WT, or P112Q/ G757D TRPC6 C-terminally fused to YFP and mCherry treated with doxycycline (Dox) (0.5g/mL) 24h before measurement. Carbachol (Cch: 100M) was added about 20s after the start of the measurement. Effects of SAR7334 (SAR; 100M) and SH045 (100M) are observed in each TRPC6 mutant. DOG (100M) was added about 20s after the start of the measurement. Effects of SAR7334 (SAR; 100M) and SH045 (100M) are observed in each TRPC6 mutant. Statistical analysis of data obtained from at least three independent experiments is presented as bar graphs. The graphs show the differences in amplitudes of the responses on Cch application Statistical analysis of data obtained from at least three independent experiments is presented as bar graphs. The graphs show the differences in amplitudes of the responses to the DOG application ( =3, meanSEM). *** 0.0001",yes
PMC5297263,Figure_9,oa_package/58/eb/PMC5297263.tar.gz,"['BMZ staining in the split skin may take either a roof pattern (band is seen toward the epidermal side of the split) or floor pattern (band is seen on the dermal side of the split) or a combined pattern (BMZ deposits on either side of the split) [].', 'Salt-spit technique showing IgG staining on the epidermal side of the split skin in bullous pemphigoid (a) and staining with IgG on the dermal side in epidermolysis bullosa acquisita (b) (fluorescein isothiocyanate, 200)Antigen identification using epidermolysis bullosa skinIn sAIBD, autoantibodies are produced against the constituents of BMZ; the same molecule may be deficient in hereditary epidermolysis bullosa (EB).']","Figure 9 Salt-spit technique showing IgG staining on the epidermal side of the split skin in bullous pemphigoid (a) and staining with IgG on the dermal side in epidermolysis bullosa acquisita (b) (fluorescein isothiocyanate, 200)",yes
PMC11510559,Figure_7,oa_package/c5/ff/PMC11510559.tar.gz,"['HE staining results (B) indicated that the WT group displayed no significant pathological changes in the hippocampus, with densely packed pyramidal cells, normal morphology, a large number of cells, normal staining, uniform distribution, orderly arrangement, and intact structure without necrotic or degenerated neurons.', 'Nissl staining (C) showed densely packed pyramidal cells in the CA1, CA3, and cortical areas, with intact structures and abundant dark blue Nissl bodies in the cytoplasm.', 'As shown in D, numerous SYN-positive cell bodies and neurites were abundant and regularly arranged in the hippocampal region of WT mice.', 'Immunofluorescence labeling for PSD95 (E) revealed a decrease in PSD95-positive cells in the hippocampal neurons of FAD4T mice compared to WT mice.', 'The Western blotting results (E,F) indicate that OUA upregulated Arg-1 expression and downregulated iNOS expression, thereby modulating the polarization phenotype of microglia in FAD4T mice.', 'OUA attenuates neuronal and synaptic damage and pathological changes and effects on signaling pathways in FAD4T mice.']","Figure 7 OUA attenuates neuronal and synaptic damage and pathological changes and effects on signaling pathways in FAD mice. ( ) Schematic diagram of mouse hippocampal structure. ( ) HE staining of the hippocampus (200); scale bar = 100 m. ( ) Nissl staining of the hippocampus (200); scale bar = 100 m. ( ) Immunofluorescence detection of SYN expression (400); scale bar = 20 m. The black boxes in the figure indicate areas of magnification or focus. ( ) Immunofluorescence detection of PSD98 expression (400); scale bar = 20 m. ( ) Quantitative analysis of SYN and PSD95 fluorescence intensity. ( ) Representative Arg-1, iNOS, TREM2, PI3K, p-PI3K, AKT, and p-AKT protein levels determined using Western blot analysis. ( ) The levels of Arg-1, iNOS, TREM2, PI3K, p-PI3K, AKT, and p-AKT. The data are presented as the means S.E.M.s, n = 3. ** < 0.01, *** < 0.001 and **** < 0.0001 compared with the corresponding control group; < 0.05 and < 0.001 compared with the corresponding model group.",yes
PMC10387951,Figure_1,oa_package/5d/17/PMC10387951.tar.gz,"['For inconclusive cases, a specific marker (CD34) was utilized (A, 1B).', '0000000000008490\n2909530929\n\nLauriaR\nPerroneF\nCarlomagnoC\nDe LaurentiisM\nMorabitoA\n\n\nThe prognostic value of lymphatic and blood vessel invasion in operable breast cancer\nCancer\n1995\n76\n10\n1772\n1778\ndoi\n8625046Lymphovascular invasion (black arrow) seen in hematoxylin-eosin (A) and CD34 (B) stained sections of breast tumors from the same case; 400.']",Figure 1 Lymphovascular invasion (black arrow) seen in hematoxylin-eosin (A) and CD34 (B) stained sections of breast tumors from the same case; 400.,yes
PMC10405251,Figure_1,oa_package/fc/03/PMC10405251.tar.gz,[],"FIGURE 1 Expression of OPN in oligodendrocytes. (A) Left panel: A representative image of fluorescent double staining of Olig2 (green) and OPN (red) with nuclear counterstain (DAPI) in the corpus callosum (delineated area) of wild type mice brain. Right panel: A magnification picture of selected area from the image in the left panel. Arrowheads: Oligodendrocytes expressing OPN. (B) Representative images of O4 oligodendrocytes isolated from control mice at P5, stained using antibodies against OPN (red) and Olig2 (green), and nuclear counterstain (DAPI). (C) Representative images of oligodendrocytes isolated from control, OPNKO, and OLsiOPNKI (iOPNKI) mice at P5 showing the expression of OPN in oligodendrocytes that were costained using antibodies against Olig2 or the endoplasmic reticulum marker calreticulin. Scale bars are 100m in (A), 50m in (B), and 10m in (C).",yes
PMC11496649,Figure_6,oa_package/85/40/PMC11496649.tar.gz,"['6a and Supplementary ', 'Effect of high-fat diet consumption on regulation at the maternal fetal interface.', 'We then compared the data from the CD and HFD groups at E14.', '6b, Supplementary ', '6c and Supplementary ', '6d and Supplementary ', '6e, f and Supplementary Table S13).', '6e, f), which have been reported to play important roles in vascular remodeling and maintenance of pregnancy at the interface62 66.', '6g).', '6e).', '6g).', '6h).', '6i), and its top target genes included several top genes belonging to factor1 (S100a8), factor8 (Jam2), and factor18 (Isg15) (', '6j and Supplementary Table S14).']","Fig. 6 Effect of high-fat diet consumption on regulation at the maternalfetal interface. Schematic diagram showing the HFD treatment schedule and time points for sampling. UMAP representation of 20 factors in the CD and HFD groups at E14.5. Spatial distributions of factors 1, 8, 17, and 18 are shown, and colors from light blue to red indicate low to high coefficients for each bin50. Proportions of immune cell types at the isolated maternalfetal interface; E10.5 S1, E10.5 S2, E10.5 S3, H10.5 S1, E14.5 S1, E14.5 S2, and H14.5 S1 sections were used for this analysis. Functional enrichment based on the top 50 weighted genes associated with factors 1, 8, 17, and 18. Top ten genes defining the indicated NNMFs (factors). RT-qPCR analysis of the expression of genes associated with factor 1 ( and ), factor 8 ( and ), factor 18 ( and ) and factor 17 ( and ). Genes that were differentially expressed in the decidual and JZ of the maternalfetal interface are shown. Gene expression level and regulon activity of in the decidua of the interface region. Top 15 target genes of in the CD and HFD groups at E14.5 are shown, and genes that were associated with factor 18 are labeled in red.",yes
PMC7642215,Figure_9,oa_package/2b/d6/PMC7642215.tar.gz,"[' shows five cases of our experimental results, in five columns.']","FIGURE 9 The patch-level visualization of image generation results. Here we list five cases in five columns. At each column, the top image is the HE pathology patch, the middle image is the corresponding Ki-67 pathology patch, and the bottom one is the synthetic Ki-67 pathology patch generated with our proposed method.",yes
PMC10646495,Figure_7,oa_package/a0/ca/PMC10646495.tar.gz,"[' Ultrasound images of the thyroid one and a half years after the chemotherapy The thyroid gland hash slight heterogeneity of the parenchyma with small focal hypoechogenicityDuring an 18-month follow-up period, no recurrence of lymphoma was reported.']",Figure 7 Ultrasound images of the thyroid one and a half years after the chemotherapy The thyroid gland hash slight heterogeneity of the parenchyma with small focal hypoechogenicity,yes
PMC9793584,Figure_3,oa_package/6c/8a/PMC9793584.tar.gz,"[' 3) [17].', 'a: A score of 0 indicates normal vertebral bodies without formation of new bone; b: A score of 1, anterior new bone formation without a solid bony bridge OR a connection between two adjacent vertebral bodies without abundantly formed bone;c: A score of 2, near complete bridging by the anterior new bone formation with less than 2 mm of distance between the bony structures or a full connection of the bone in a maximum of two sagittal or coronal CT sections; d: A score of 3, complete bridging between the vertebral bodies above and below the disc with abundant new bone formation in more than two sagittal or coronal CT sectionTwo authors, who were blinded to the patients, diagnosed DISH according to the Resnick and Niwayama s criteria: At least four contiguous vertebral segments was affected , sacroiliac joint was not affected, the absence of apophyseal joint ankyloses, and preservation of intervertebral disc spaces [3].']","Fig. 3 The bridge scores were evaluated in sagittal CT image. A score from 0 to 3 was assigned for each vertebral segment. a: A score of 0 indicates normal vertebral bodies without formation of new bone; b: A score of 1, anterior new bone formation without a solid bony bridge OR a connection between two adjacent vertebral bodies without abundantly formed bone;c: A score of 2, near complete bridging by the anterior new bone formation with less than 2mm of distance between the bony structures or a full connection of the bone in a maximum of two sagittal or coronal CT sections; d: A score of 3, complete bridging between the vertebral bodies above and below the disc with abundant new bone formation in more than two sagittal or coronal CT section",yes
PMC3372551,Figure_1,oa_package/d3/0e/PMC3372551.tar.gz,"['A lumbar magnetic resonance imaging (MRI) demonstrates an well defined round intrathecal mass, very well depicted in the T1 and T2 WI (A).', 'Axial MR slice (B) show significant compression of thecal sac at the level of L4-L5, with a central slightly hyperintense signals changes.', 'A report of two casesSpine J2007711111717197344(A) T2-weighted saggital magnetic resonance imaging at L4-L5 level of a 30 years old male shows thecal sac compression and hyperintense signals inside the sac (arrows).']",Fig. 1 T2-weighted saggital magnetic resonance imaging at L4-L5 level of a 30 years old male shows thecal sac compression and hyperintense signals inside the sac (arrows). T2-weighted axial section shows slightly hyperintense mass in central position in the dural sac (arrow).,yes
PMC2895168,Figure_1,oa_package/40/f8/PMC2895168.tar.gz,['A propos de deux casChirurgie19991243073121042930614ScerpellaPRBudensteinerJAGiant sigmoid diverticulaArch Surg198912412441246280299015NaberASliutzAMFreitasHGiant diverticulum of the sigmoid colonInt J Colorectal Dis1995101691727561437Double-contrast enema.'],Fig. 1 Double-contrast enema. A giant diverticulum arising from the mid-sigmoid colon.,yes
PMC5968303,Figure_3,oa_package/81/76/PMC5968303.tar.gz,['.'],"Fig. 3. Immunohistochemical staining of the ampullary tumor, with benign tissue on the left border of each image. Positive staining for CK-19 ( ), CK-20 ( ), MUC-1 ( ), MUC-2 ( ), CDX-2 ( ), DPC-4 ( ), and negative for MUC-5ac ( ).",yes
PMC8626648,Figure_3,oa_package/e9/38/PMC8626648.tar.gz,"['Apical 4-chamber view revealed a large cavity (30 21 mm in diameter) adjacent to the mitral valve communicating with the left ventricle via single neck, but not with the left atrium, clogged with thrombus and moderate pericardial effusion not compromising right ventricular function ().', 'TTE; A: apical four chamber showing the aneurysm (red arrow), the thrombus (blue arrow) and the pericardial effusion (white arrow).', ')']","Fig. 3 TTE; A: apical four chamber showing the aneurysm (red arrow), the thrombus (blue arrow) and the pericardial effusion (white arrow). B: parasternal long axis view demonstrating the submitral aneurysm. LV: left ventricle, LA: left atrium. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)",yes
PMC9689675,Figure_1,oa_package/b4/7c/PMC9689675.tar.gz,"['Additionally, there were large numbers of CD68+ macrophages ().', '202100593035443023Histopathological features of muscle biopsy.']",Figure 2 This figure represents gender and age distribution among the described cases.,yes
PMC6738441,Figure_1,oa_package/2c/ff/PMC6738441.tar.gz,"['7%) had other combinations of the three biomarkers (A).', '2%) had other combinations (B).', '5%) were triple-negative (C).', 'The triple-positive group had favorable prognosis, whereas the TERT mutation group had a dismal survival expectancy (D, univariate analysis in Supplementary Table 2), although this relationship was not found for Grade IV gliomas (E, Supplementary Table 5).', 'Proportion and Kaplan-Meier survival analyses of molecular groups in diffuse infiltrative gliomas.', 'In contrast, none of the hematological markers impacted OS in the triple-positive group of lower-grade gliomas (Supplementary A 1D).', 'Likewise, no prognostic significance was found for PLR and AGR in the IDH and TERT mutation group (Supplementary E, 1F), MLR and AGR in the IDH mutation only group (Supplementary G, 1H), MLR and AGR in the TERT mutation only group (Supplementary A, 2B), and PLR, MLR, and AGR in the triple-negative group (Supplementary C 2E) of lower-grade gliomas.']","Figure 1 Survival proportions in infiltrative (Grade II-IV) gliomas ( ), lower-grade (Grade II-III) gliomas ( ), and Grade IV glioma ( ). ( ) Kaplan-Meier OS curves in lower-grade gliomas. OS estimates for the 5 molecular groups are significantly different (P < 0.001). ( ) Kaplan-Meier OS curves in Grade IV glioma. No differences in OS were detected for the 3 molecular groups (P = 0.285).",yes
PMC4882386,Figure_4,oa_package/ba/16/PMC4882386.tar.gz,"['Control chest X-ray revealed reduced size of the pseudoaneurysm and proper apposition of the vascular coil ().', 'Right angiogram: the metal coil (white arrow) in the perforated artery occluding the feeding vesselCase 1.']",Figure 4 Case 1. Chest X-ray (A-P position). Allocation of the metal coil (white arrow) and reduction of the pseudoaneurysm,yes
PMC4635572,Figure_5,oa_package/e2/3d/PMC4635572.tar.gz,"[' 5).', '001 relative to sham ratsTop row shows EPIC micro-computed tomography ( CT) images of the articular cartilage covering the medial tibial plateau from rats assign to study group 5A to 5D.', 'Second row depicts large cartilage defects extending over the lateral half of Zone 1 and medial half of Zone 2, as indicated by arrows in three-dimensional CT color thickness ( heat ) map of the articular cartilage at the medial tibial plateauHistologyHistological evaluation of the articular cartilage revealed classic images of cartilage degradation caused by the MM surgery, including a thinning to complete absence of the cartilage due to chondrocyte death or atrophy, cartilage fibrillation and the presence of osteophytes.']","Fig. 5 Top row shows EPIC micro-computed tomography (CT) images of the articular cartilage covering the medial tibial plateaufrom rats assign to study group 5A to 5D. indicate cartilage defects present in all three groups of operated rats. Second row depicts large cartilage defects extending over the lateral half of Zone 1 and medial half of Zone 2, as indicated by in three-dimensional CT color thickness (heat) map of the articular cartilage at the medial tibial plateau",yes
PMC6018778,Figure_3,oa_package/2a/ad/PMC6018778.tar.gz,"['MRI ImagesAll 16-wk group animals had no persistent signs of cerebral compression, CSF leakage, hydrocephalus, infection, or hemorrhage ().', 'FIGURE 3.', 'Macroscopic Tissue EvaluationThere were no abnormal macroscopic findings associated with either dural substitute at any time point.']","FIGURE 3. MRI of dogs treated with DuraGen XS, a collagen dural substitute, or Hemopatch, a polyethylene glycol-coated collagen dural sealant and substitute. Serial coronal and sagittal T1- and T2-weighted images were obtained to investigate adverse effects on the brain. Images were obtained preoperatively and postoperatively, and at weeks 1, 8, and 16. Neither collagen duraplasty material is present on MRI. Cerebral compression secondary to the surgical procedure is seen on postoperative images in both groups, which resolves in subsequent MRI. Neither duraplasty material was associated with persistent signs of CSF leak, hydrocephalus, infection, or hemorrhage up to 16 wk after application to the durotomy.",yes
PMC6614545,Figure_5,oa_package/c4/ae/PMC6614545.tar.gz,"['Immunohistochemistry was as follows:Estrogen receptor (clone SP1, Cell Marque) - IS +++, 3 points; PS 40%, 4 points; TS = 7 points; Progesterone receptor (clone Y85, Cell Marque) - IS +++, 3 points; PS 40%, 4 points; TS = 7 points ().', 'Immunohistochemical study (diaminobenzidine, hematoxylin), x200 magnification.', '3DiscussionM.']","Fig. 5 Immunohistochemical study (diaminobenzidine, hematoxylin), x200 magnification. Tumor cells express the progesterone receptor (Y85 clone, Cell Marque).",yes
PMC5508651,Figure_1,oa_package/aa/47/PMC5508651.tar.gz,"['1) [32].', 'Sexual stage commitment of P.', '05\nABA reduced Plasmodium infection in the mosquito by increasing TAK1-regulated host immunityWe previously showed that ABA supplementation increased expression of defensin, apl1, and nos in the midgut of A.', '11).', '1Model of ABA signaling within the mosquito midgut upon ingestion of a P.', 'Reduced levels of ilp3 and ilp4 are conducive to increased nos expression and reduced infection, but are short-lived and have no effect on fecundity\nWithin 30 min to 1 h post-feeding, ABA induced changes in phosphorylation and activity of major immune and metabolic regulators in the mosquito midgut, including TAK1, AMPK, and GSK-3.']",Fig. 1 Sexual stage commitment of is restricted by ABA treatment within 24h of mosquito infection. Expression levels of genes and in ABA-supplemented parasite-fed mosquito midguts were normalized to mosquito gene and parasite gene expression. Each dot represents one replicate of 10 pooled midguts. Data are shown as fold change relative to control and were analyzed by Students t-test. * 0.05,yes
PMC4212118,Figure_3,oa_package/05/4a/PMC4212118.tar.gz,"['On noise-filtered interictal recordings, APP23 mice showed markedly fewer events of CFC coinciding with peaks of the gamma amplitude envelope and the theta phase, as compared with non-transgenic mice ( 3A).', 'Comodulogram plots of non-transgenic mice show several frequency pairs with significant coupling, in particular at ~6 Hz ( 3B).', 'In contrast, APP23 comodulogram plots showed markedly lower coupling intensities across theta frequencies ( 3B).', 'To quantify CFC in relation to theta phase, we plotted phase-amplitude distributions for 1-minute segments of hippocampal recordings for APP23 and non-transgenic controls during the active phase of the light cycle ( 3C).', 'Non-transgenic phase-amplitude distributions peak in synchrony with theta phase indicative of significant CFC ( 3C).', 'However, phase-amplitude distributions in APP23 recordings did not show a significant peak during the theta phase ( 3C).', 'APP23 recordings used to calculate phase-amplitude distributions had a significantly lower modulation index compared to recordings from non-transgenic mice ( 3D; n = 5 animals 2-3 measurements each, t = 2.', '\nGamma amplitude modulation by theta phase is impaired in APP23 mice.', 'Riluzole-induced acute hippocampal hypersynchronicity in APP23 miceRiluzole injection was shown to induce cortical hypersynchronicity in APP transgenic J20 mice [34].']","Figure 3 Raw EEG (LFP), band pass filtered signals for theta (4-12Hz) and gamma (25-100Hz) oscillations, gamma amplitude envelope (green) and theta phase in APP23 and non-transgenic (non-tg) mice. Representative signals from 5 animals per genotype are shown. Representative phase-amplitude comodulograms computed for hippocampal LFPs recorded in non-transgenic (non-tg) and APP23 mice. Phase-amplitude plot computed for hippocampal LFPs recorded in non-transgenic (non-tg) and APP23 mice. (n=5) meanss.e.m. Modulation index computed for the phase-amplitude distributions shown in . (n=5) meanss.e.m. *p<0.05 (Students t-test).",yes
PMC5183928,Figure_1,oa_package/e6/f6/PMC5183928.tar.gz,['Secondary middle turbinate is apparent on the right side of the figure.'],Figure 1 Secondary middle turbinate is apparent on the right side of the figure. smt: Secondary middle turbinate; mt: Middle turbinate.,yes
PMC10680192,Figure_5,oa_package/47/ff/PMC10680192.tar.gz,"[' 5.', 'Post-mortem casesCAA, cerebral amyloid angiopathy; PSEN, presenilin; AD, Alzheimer s disease; FTLD, frontotemporal lobar degeneration; CBD, corticobasal degenerationDiscussionIn the current work, we investigated longitudinal clinical outcome measures in a population consisting primarily of early-onset dementia patients who underwent amyloid-PET imaging during their initial diagnostic work-up.']","Fig. 5 Post-mortem cases CAA,cerebral amyloid angiopathy; PSEN,presenilin; AD, Alzheimers disease; FTLD,frontotemporal lobar degeneration; CBD, corticobasal degeneration",yes
PMC9453041,Figure_11,oa_package/ba/61/PMC9453041.tar.gz,[],"Figure11 Expression of vWF in livers of mice infected by , identified by immunofluorescence. Detection of more than one megakaryocyte among the hepatocytes in the same area of analysis at 45 dpi; Blood vessel vWF-positive with inflammatory cells in the periphery situated between the endothelium and the hepatocytes at 45 dpi; Megakaryocytes (arrow) are still found in the liver of infected animals during the acute phase of the disease, close to the peripheral zone of the granulomas, but still in contact with hepatocytes, and close to a vessel with surrounding hematopoietic cells at 50 dpi; central vein (CV), vessels associated with the periphery of the granuloma and some sinusoids express the protein at 60 dpi. Bars, : 50 m, 20 m, D: 10 m. DAPI: white, Evans blue: red, vWF: green.",yes
PMC2704894,Figure_2,oa_package/91/ef/PMC2704894.tar.gz,"['Characteristic images of various findings are shown in .', 'Representative sections of the radial artery showing different histopathological findings encountered in the study.']","Figure 2 Representative sections of the radial artery showing different histopathological findings encountered in the study. IH, intimal hyperplasia; M, media.",yes
PMC6002328,Figure_3,oa_package/8b/8c/PMC6002328.tar.gz,"[' 3).', 'Each branch of the hepatic artery, portal vein, and biliary duct were ligated together as the Glissonean bundle (so-called FSTG) (arrow)Three months later, dynamic MRI was performed to check for intrahepatic recurrence, and no imaging findings of recurrence were observed.']","Fig. 3 Intraoperative findings of FSTG. Anterior segmentectomy was performed. Each branch of the hepatic artery, portal vein, and biliary duct were ligated together as the Glissonean bundle (so-called FSTG) (arrow)",yes
PMC5577311,Figure_3,oa_package/fc/e1/PMC5577311.tar.gz,"['Immunohistochemical analysis demonstrated the localization of multiple BLBP-positive stem cells in the SGZ of both WT and APP/PS1 mice at 4-, 6- and 12-months of age (Figs 3A and 4A).', ' 3).', ' 3A a4 6 and ', ' 3B).', 'BLBP-positive stem cells in the SGZ in WT and APP/PS1 mice.', '\nBLBP-positive stem cells are reduced in aging and in APP/PS1 mice.']",Figure 3 BLBP-positive stem cells in the SGZ in WT and APP/PS1 mice. ( ) Light microscopic images of the dentate gyrus of WT (a1a3) and APP/PS1 (a4a6) mice immunostained for BLBP. ( ) Double confocal images of GFAP (b13) and BLBP (b46) markers demonstrate the presence of radial glial-like stem cell type-1 progenitors in both WT and APP/PS1 mice as shown in the co-localization images (b79). g: granular cell layer; h: hilus; m: molecular cell layer. Scale bars: 25m (a1a6 and b1b9).,yes
PMC8544838,Figure_3,oa_package/54/6a/PMC8544838.tar.gz,"['IFA on fixed cells demonstrated a punctate distribution of KAHRP in cells infected with both wild type and mutant cell lines (A).', 'ref033"" ref-type=""bibr"">33] revealed that structures labelled for KAHRP were equally numerous but statistically larger in diameter in RBCs infected with PFA than with CS2 (B and 3C).', 'Surprisingly, considering the relatively low resolution of live cell epifluorescence microscopy, (but preserving membrane integrity and cell morphology), structures labelled with KAHRP::mCherry could be seen to emerge from the surface of RBCs infected with PFA, possibly representing eKnobs (D, Representative composite fluorescence images of the uptake of the perfusion marker Hoechst 33342 in whole tumour sections from orthotopic PC3 prostate xenografts treated with (A) vehicle or (B) 25 mg kg 1 saracatinib daily over 5 days.']","Figure 2 Representative composite fluorescence images of the uptake of the perfusion marker Hoechst 33342 in whole tumour sections from orthotopic PC3 prostate xenografts treated with ( ) vehicle or ( ) 25mgkg saracatinib daily over 5 days. The mean Hoechst 33342 perfused area was 5.140.9% and 4.840.8% for the saracatinib-treated and vehicle cohorts, respectively, and there was no significant difference between them. ( ) Representative high power and whole section images showing immunohistochemical staining for pFak Y861, Fak, pPaxillin Y31 and paxillin in orthotopic PC3 prostate xenografts from mice treated with vehicle or 25mgkg saracatinib daily over 5 days.",yes
PMC10543728,Figure_2,oa_package/65/22/PMC10543728.tar.gz,"['The time-course changes of Morrbid in the infarcted mouse hearts within 24 hours after AMI was shown in Supplemental .', 'As shown in , A and B, the Ad-Morrbid treated group had better ejection fraction (EF) and fractional shortening (FS), as well as smaller left ventricular end-diastolic dimension (LVEDD)and left ventricular end-systolic dimension (LVESD), compared with the Ad-GFP treated group.', 'In contrast, cardiomyocyte-specific Morrbid-KO (Morrbidfl/fl/Myh6-Cre) mice had the worse EF and FS, and larger LVEDD and LVESD, compared with the control Morrbidfl/fl group (, C and D).', 'Representative echocardiographic images obtained from 4 groups of mice were shown in , A and C.', 'The effect of Morrbid on cardiac function at 24 hours after AMI.']","Figure 2 The effect of on cardiac function at 24 hours after AMI. ( and ) Representative echocardiographic images obtained from 4 groups of mice. ( ) Echocardiographic measurements for Ad- treated mouse hearts and for Ad-GFPtreated control mouse hearts: EF ( < 0.001), FS ( < 0.001), LVEDD ( = 0.0015), LVESD ( < 0.001). ( ) Echocardiographic measurements for -KO mice ( /Myh6-Cre) and their control mice ( ): EF ( < 0.001), FS ( < 0.001), LVEDD ( = 0.1618), LVESD ( = 0.0332). Ad-GFP, = 24; Ad- , = 20; , = 12; /Myh6-Cre, = 10. * < 0.05; ** < 0.01; *** < 0.001 by unpaired 2-tailed Students tests.",yes
PMC11298331,Figure_1,oa_package/37/21/PMC11298331.tar.gz,['Radiological image after DJ ureteral stentingDJ: Double-JThe patient was informed about the condition of his right kidney.'],Figure 1 Radiological image after DJ ureteral stenting DJ: Double-J,yes
PMC11581895,Figure_4,oa_package/7b/19/PMC11581895.tar.gz,"['After further multidisciplinary discussions, the decision was made to proceed with primary radiotherapy (A).', '(A) Primary radiotherapy for ependymoma contours and isodose distribution.', 'Repeat MRI with perfusion-weighted imaging was performed on postoperative day 100 (B) and indicated a combination of residual/recurrent enhancing tumor and necrosis.', 'Subsequent imaging on postoperative day 195 (B) revealed an increased extent of enhancing tissue along with central enhancement, FLAIR abnormality, and mass effect, highly suspicious for tumor progression.', 'Postoperative MRI revealed expected postoperative changes and significant residual disease (B).']",Figure 4 Primary radiotherapy for ependymoma contours and isodose distribution. Ependymoma post-operative imaging.,yes
PMC8536526,Figure_1,oa_package/d7/c7/PMC8536526.tar.gz,"['9 cm thick, and the left ovary with normal shape and size (A).', '5 cm, according to the picture of a solid ovarian neoplasm, possibly a GCT (B).', 'Oncology ultrasound results showed (A) retroflexed uterus 7.', 'The patient then underwent a whole abdomen CT scan (']","Fig. 1 Oncology ultrasound results showed (A) retroflexed uterus 7.94.25.3cm in size, 97.7mL in volume, homogeneous myometrium with a regular endometrial line with a thickness of 11.9mm. (B) Solid mass with a regular outer surface, increased vascularity color score 3, with an acoustic shadow, the overall size of the tumor mass is 10.412.913.5cm, volume is 966.7mL possibly a granulosa cell tumor.",yes
PMC5573450,Figure_4,oa_package/9f/5b/PMC5573450.tar.gz,['.'],Figure 4. Sinusitis of frontal sinus.,yes
PMC6765162,Figure_8,oa_package/39/a7/PMC6765162.tar.gz,"['66T1 and T2 weighted MRI show a hypointense lesion with septal enhancement ().', '66X-ray of the (left- 2 red arrows) shoulder show opacified cystic, lobulated peri-articular lesions in Tumoral Calcinosis.']","Figure 8 X-ray of the (left- 2 red arrows) shoulder show opacified cystic, lobulated peri-articular lesions in Tumoral Calcinosis. Coronal MRI T2 sequencing (right-red arrow) reveals hypointense lesions with septal enhancement, hyperintense fluid filled cavities and fluid fluid levels consistent with sedimentation.",yes
PMC10837233,Figure_5,oa_package/21/b7/PMC10837233.tar.gz,"[' 5A, B).', 'aTotal43557492145/492 (29%)29%131/145 (90%)131/435 (30%)14/57 (24%)Preoperative neurological deficits were present in the vast majority of cases (A), which showed heterogeneous development over time after surgery (B).', 'No significant differences were seen in age dependent distribution of postoperative neurological deficits, however with relatively lower rates of progressive or persistent neurological deficits in infants at an age 1 year and relatively higher rates in children with the age between 1 and 3 years (E)Postoperatively, 474 cases (89.', ' 5C, D).']","Fig. 5 Preoperative neurological deficits were present in the vast majority of cases ( ), which showed heterogeneous development over time after surgery ( ). New neurological deficits after surgery were observed in 10.7% of cases ( ), which developed postoperatively more favorable compared to preexisting neurological deficits with 5.3% resolving, 2.7% regressive, and 2.8% persistent deficits ( ). No significant differences were seen in age dependent distribution of postoperative neurological deficits, however with relatively lower rates of progressive or persistent neurological deficits in infants at an age<1year and relatively higher rates in children with the age between 1 and 3years ( )",yes
PMC2629687,Figure_3,oa_package/01/09/PMC2629687.tar.gz,"['Microscopic examination revealed that the anterior chamber was filled with serosanguinous fluid ().', 'Hemorrhagic RPE detachment was found.']","Fig. 3 Hemorrhagic RPE detachment was found. Blood also filled the subretinal space (asterisk) (hematoxylin-eosin stain: original magnification, 400).",yes
PMC6883668,Figure_2,oa_package/20/8e/PMC6883668.tar.gz,"['Final pathology of the biopsy was consistent with a high-grade HCCC with ductal and\nmucinous differentiation and necrosis ().', '.', '1177_2152656719889030-fig2""/>Given the diagnosis and positive margins, the patient underwent intensity-modulated\nradiation therapy of 66 Gy targeting the nasopharynx and regional lymphatics over 6\nweeks of duration.']","Figure 2. Tumor histology. A, H&E 40 low-power view demonstrating an infiltrativecarcinoma growing in solid nests and cords with areas of glandular formationin a background of hyalinized stroma. B, H&E 100 multiple areas ofglandular formation (ductal differentiation) present in a background ofhyalinized stroma. Areas of cytoplasmic clearing more typical of HCC can beappreciated in the more solid growth pattern. C, H&E 100 focal areas ofthe tumor demonstrated more classic morphology, with clear cells juxtaposedto peritumoral hyaline stroma. D, H&E 200 minor component consisting ofclear cells juxtaposed to peritumoral hyaline stroma. E, H&E 200evidence of mucinous differentiation with mucin production.",yes
PMC9340122,Figure_5,oa_package/28/3d/PMC9340122.tar.gz,['Histologic examination showed numerous nonanastomosing blood vessels as well as endothelial proliferation ('],"Fig. 5 Postembolization angiogram. (A) Magnified lateral view of distal left external carotid artery showing embolic material (black arrow) without any hypervascular blush. (B) Unmagnified left common carotid artery angiogram, lateral view, showing reduction of preoperative blush without any off-target embolization.",yes
PMC2039791,Figure_4,oa_package/35/f2/PMC2039791.tar.gz,"[' 4).', '', 'Marker bar in (d) = 500 mOn microscopy, regressive changes are present in all, with the vermis relatively spared.', ' 4).']","Fig.4 Vermis cerebelli, case 5. Stains, HE, GFAP, neurofilament protein, vimentin. Adjacent sections with circumscript segments of loss of all layers and glial reaction. in ( ) point to circumscript areas of lost cortex. Sharply demarcated foci and replacing bands of astrocytes possibly modified Bergmann cells. in ( )=500m",yes
PMC7586607,Figure_2,oa_package/c8/cb/PMC7586607.tar.gz,"['Consistency was soft, tenderness was present, and no bleeding or pus discharge on manipulation; similar swelling of smaller size was present 1 cm anterior to it [].']",Figure 2 Two swellings present in the lower right chin region,yes
PMC9656965,Figure_5,oa_package/96/23/PMC9656965.tar.gz,"['For further tests, models of three different RFA catheter positions were created, see .', 'Three Gaussian-type UWB pulses (1 4 GHz, 1 6 GHz, and 1 10 GHz) using the heterogeneous model with the catheter in position 1 according to were tested, and the 2D reconstructions were carried out.', 'Numerical Catheter Position StudyThe modified system was tested on the heterogeneous model for three different catheter positions in the following study, according to .', 'Tested positions of the RFA catheter within the heterogeneous numerical model.']",Figure 5 Tested positions of the RFA catheter within the heterogeneous numerical model. The solid yellow line represents the RF catheter.,yes
PMC3137188,Figure_5,oa_package/72/1a/PMC3137188.tar.gz,[],"Fig. (5) Representative examples of lanthanum extravasation across the blood-brain (a) and blood-spinal cord (b) barriers following 48 h after spontaneous morphine withdrawal in dependent rat. Infiltration of lanthanum is seen (arrowheads) across the endothelial cell of one microvessel from the cerebral cortex (a) and from the cervical spinal cord (b). Occurrences of many microvesicular profiles are clearly evident in the spinal cord endothelial cells (b). The tight junctions appear to be closed for lanthanum (arrows) in the cerebral (c) and spinal cord (d) microvessels. Edematous swelling of perivascular astrocytes is clearly visible (a-d). Bar = 500 nm (a); 600 nm (b), 300 nm (c); 400 nm (d).",yes
PMC7044467,Figure_4,oa_package/e4/d4/PMC7044467.tar.gz,"['However, the MRI failed to classify the patient s M llerian anomaly and was read as a right enlarged pelvic mass, possible endometrioma of ovary, with displaced uterus to left pelvic area ().', 'The coronary plane of MRI with IV contrast: A.', 'The patient underwent an exam under anesthesia, diagnostic hysteroscopy as well as diagnostic laparoscopy.']","Fig. 4 The coronary plane of MRI with IV contrast: A. Bladder, B. Hematometra in the rudimentary non-communicating horn with functioning cavity, misread as right ovarian cyst structure, C. Left unicornuate uterus.",yes
PMC11221180,Figure_6,oa_package/41/40/PMC11221180.tar.gz,"[' 6) were compared with the EfficientNet-B0-V1 model as shown in Table 4.', '889\n\nConfusion matrix of ResNet18, MobileNet-V2, and EfficientNet-B0-V2 models (from left to right) on the validation set\nThe overall accuracy of a whole image can be obtained by combining the diagnostic results of each partitioning by Table 5.']","Fig. 6 Confusion matrix of ResNet18, MobileNet-V2, and EfficientNet-B0-V2 models (from left to right) on the validation set",yes
PMC7444851,Figure_3,oa_package/b8/c1/PMC7444851.tar.gz,['CT shows near resolution of retroperitoneal fibrosis depicted with the arrow.'],Figure 3 CT shows near resolution of retroperitoneal fibrosis depicted with the arrow.,yes
PMC11717790,Figure_5,oa_package/82/a9/PMC11717790.tar.gz,"[' 5).', 'This technique is particularly helpful in patients with a high or very thick palate, who would normally need 2 or even more axial images to obtain the indexResults3T MRI of the midface was successfully performed in 30 patients (24 93 years; 15 females, 15 males; mean age 54 17).', ' 5).', ' 5).']","Fig.5 Displays the curved MPR reconstruction of the mid-palatal suture in one plane . This technique is particularly helpful in patients with a high or very thick palate, who would normally need 2 or even more axial images to obtain the index",yes
PMC3631315,Figure_4,oa_package/99/7e/PMC3631315.tar.gz,"[' 4a, S4).', ' 4b).', '', 'Values represent mean SEM\nTo determine whether these morphological changes were associated with structural changes in htauWT, we performed additional biochemical analyses on whole brain extracts from aged flies expressing TH::htauWT.', ' 4c).', ' 4d), but not in identically prepared sarkosyl-insoluble fraction from age-matched nontransgenic control brains.', ' 4d), resembling those PHFs found in AD [12].', ' 4e).', 'Related to .']","Fig.4 Expression of htau produces AD-like abnormal tau phosphorylation and NFT-like pathology. Single cell of PPL1 and PPM3 DA neurons from fly brains at 6weeks of age. Compared to the age-matched control DA neurons from PPL1 cluster labeled with GFP ( ), immunostaining of three antibodies that recognize AD-like hyperphosphorylated tau (AT8 , AT100 , AT180 ), and a polyclonal tau antibody ( ) reveal tangle-like pathology in the degenerating DA neurons from PPL1 ( ) and PPM3 ( ) clusters. The degenerating DA neurons from both PPL1 and PPM3 clusters are stained with Congo red. The time course of pathological tangle-like structure formation in htau brains during the aging process is presented in Fig. S3. Representative immunoblot of polyclonal anti-htau shows that a substantial amount of htau proteins are converted from monomer tau (75kDa) to the oligomeric Tau species (>400kDa). Anti--tubulin serves as a loading control. Protein expression levels from four independent immunoblots with monomeric and oligomeric htau protein levels calibrated against the loading control are shown as relative expression. Values shown represent meanSEM. Electron micrographs of abnormal filaments extracted from tangle-rich preparation (sarkosyl-insoluble pellets) of fly brains expressing htau . Images show several PHF-like filaments are bundled ( ), a single PHF ( ), and a straight filament ( ). 100nm. Representative immunoblot from sarkosyl-insoluble pellets reveals monomer tau ***(75kD) and the htau-containing species with molecular weights range between 75 and 400kD (~90, ~160 and ~240kD). Anti--tubulin also serves as a loading control. Monomeric and polymeric htau protein levels are quantified by analyzing three independent immunoblots and shown as relative expression. Values represent meanSEM",yes
PMC9768537,Figure_2,oa_package/88/b9/PMC9768537.tar.gz,"['(A) preoperative forntal CT indicating that there were multiple cystic low-density shadows in the lower part of the right femur, with clear borders; (B) preoperative horizontal cross-sectional CT indicating that there were multiple cystic low-density shadows in the lower part of the right femur, with clear borders, sclerosis margins around, and no obvious abnormalities in adjacent soft tissues.']","Figure 2 ( ) preoperative forntal CT indicating that there were multiple cystic low-density shadows in the lower part of the right femur, with clear borders; ( ) preoperative horizontal cross-sectional CT indicating that there were multiple cystic low-density shadows in the lower part of the right femur, with clear borders, sclerosis margins around, and no obvious abnormalities in adjacent soft tissues.",yes
PMC10491916,Figure_4,oa_package/67/2c/PMC10491916.tar.gz,"['58 McElroy et al found that exogenous treatment of Nrg4 could reduce the severity of necrotizing enterocolitis (NEC) in rats, and the intraperitoneal injection of Nrg4 could completely block the drug-induced NEC (', ' 4The effects of Nrg4 on inflammatory diseases, obesity, insulin resistance, diabetes, and cardiovascular diseases.', '19 Nrg4 was able to reduce TNF , Cxcl1, and IL-1 expression in macrophages (', '19 The above pathway requires the involvement of the hydrolyzed ICD of ErbB4 receptor, which binds to mitochondria and thus stimulates apoptosis in M1 macrophages (', 'Nrg4 can inhibit JNK phosphorylation and reduce chondrocyte apoptosis (', 'Nrg4-mediated inhibition of JNK signaling also resulted in the reduction of the matrix metalloproteinase 13 (MMP-13, an enzyme for the degradation of COL II), thus helping to elevate the level of COL II (', '2"">Obesity, insulin resistance, and diabetesNrg4 is an adipokine that is sensitive to metabolic conditions and has a potential role in the combat against overweight and obesity (', '64 The expression of NF- B and its downstream inflammatory factors (including TNF , IL-1 , IL-6, and IFN- ) was significantly elevated in Nrg4 KD (shNrg4) 3T3-L1 adipocytes, and treatment with recombinant Nrg4 can suppress the inflammation in adipocytes (', '57 Taken together, by using cellular and animal models, Nrg4 was shown to ameliorate insulin resistance (', '3"">Cardiovascular diseasesNeuregulins have been shown to be beneficial in the prevention of cardiovascular disease (CVD), and Nrg4 also appears to have a similar function (']","Figure4 The effects of Nrg4 on inflammatory diseases, obesity, insulin resistance, diabetes, and cardiovascular diseases. In inflammatory bowel disease, Nrg4 can activate PI3K/AKT signaling pathway, which in turn promotes colonic epithelial cell survival. In addition, Nrg4 can promote M1 macrophage apoptosis through ErbB4 ICD binding to mitochondria, which reduces the production of TNF, Cxcl1, and IL-1 from macrophages. In osteoarthritis, Nrg4 inhibits the activation of JNK phosphorylation and reduces chondrocyte apoptosis. Moreover, Nrg4-mediated inhibition of JNK signaling also results in the reduction of the matrix metalloproteinase 13 (MMP-13), an enzyme for the degradation of type II collagen (COL II), thereby inhibiting the degradation of COL II in chondrocytes. Nrg4 may have an anti-obesity effect. In addition, Nrg4 can increase Glut4 levels on the plasma membranes to promote glucose transport and uptake in 3T3-L1 adipocytes. Nrg4 suppresses the activation of the transcription factor NF-B and the expression of its downstream inflammatory factors (including TNF, IL-1, IL-6, and IFN) in 3T3-L1 adipocytes. Besides, Nrg4/ErbB4 signaling leads to increased adipose tissue angiogenesis to improve adipose tissue function and preserve metabolic health. These effects above help to ameliorate insulin resistance. The beneficial role of Nrg4 in diabetes needs further exploration. In cardiovascular disease, BAT-derived Nrg4 activates Akt signaling through ErbB4 receptor, which inhibits the NF-B activity to reduce endothelial inflammation and injury, thereby ameliorating atherosclerosis. In addition, the levels of circulating Nrg4 were significantly elevated after exercise, suggesting the potential roles of Nrg4 in exercise-mediated metabolic health.",yes
PMC11342184,Figure_2,oa_package/53/68/PMC11342184.tar.gz,"['The femoral vessels are displaced posteromedial ().', '']",Fig. 2 Intramuscular lipoma of the proximal right thigh ( ).,yes
PMC4608380,Figure_7,oa_package/f8/f3/PMC4608380.tar.gz,"['13 16, 50 150 kDa) tau levels (Supplementary ).', 'Phosphorylation of tau correlates with neuronal uptakeWe tested the idea that tau phosphorylation or, simply, size of tau, was important for cellular uptake by preparing a monomer-dimer-oligomer tau mixture from recombinant human WT full-length tau (441 aa), separated by SEC (a).', 'We then incubated each SEC fraction of this non-phosphorylated tau mixture (b) with mouse primary neurons.', 'No uptake was observed in primary neurons even from HMW tau fractions (c).', 'Phosphatase treatment dephosphorylated tau in rTg4510 brain extract (d) without changing HMW tau levels (', '7e), resulting in a significant reduction of cellular uptake of tau (f).', 'Phospho-tau specific antibodies were less efficient at immunodepletion than the total tau antibody (HT7) (g); however, some phospho-tau antibodies (pS199, pT205 and pS396) more efficiently reduced the neuronal tau uptake (', 'Three lines of data support the idea that phosphorylation is important: uptake efficiency correlates with extent of phosphorylation (Figs 2 and 6); enzymatically dephosphorylating the tau blocks uptake (d f); and some phospho-tau specific antibodies are able to block uptake despite being relatively ineffective in IPing tau from the rTg4510 brain extract (', 'In addition, recombinant non-phosphorylated tau, even when prepared as a HMW oligomer, is not taken up efficiently by neurons (a c).', '35 mg ml 1) with 2 mM DTT for 2 days at 37 C, followed by SEC separation and ELISA measurement of tau (a).', 'org/1999/xlink"" xlink:href=""ncomms9490-f6""/>Tau phosphorylation correlates with cellular uptake.']","Figure 7 Tau phosphorylation correlates with cellular uptake. ( ) Non-phosphorylated HMW tau was not taken up by neurons. ( ) Tau oligomer mixture solution was prepared from recombinant human tau, followed by SEC and tau ELISA. ( ) Phospho-tau levels in SEC fractions and brain extracts (pS396 tau ELISA). ( ) Each SEC fraction was incubated with primary neurons. Neurons were immunostained at day 2. ( ) Dephosphorylation reduced tau uptake. ( ) Immunoblot analysis of total (Tau13)- and phospho-tau (pS396) levels in rTg4510 (12-month-old) brain extracts treated with lambda phosphatase. ( ) SDD-AGE analysis of brain extracts treated with phosphatase. ( ) Tau uptake assay. Phosphatase-treated brain extract was applied to HEK-tau-biosensor cells. ( =3, ** <0.01), unpaired -test. ( ) Immunodepletion of phospho-tau reduced neuronal tau uptake. rTg4510 (12-month-old) brain extracts were immunodepleted with total- or phospho-tau specific antibodies. ( =5). ( ) Total tau levels in tau-immunodepleted samples (ELISA). ** <0.01 versus control IgG. ( ) Tau uptake in primary neurons (day 2). * <0.05 versus control IgG. ( ) Blocking efficiency was defined as the percentage of tau-uptake reduction (versus control IgG) multiplied by tau levels in the immunodepleted brain extracts (% control IgG). * <0.05 versus total tau (HT7). One-way ANOVA and a subsequent Tukey-Kramer test. ( ) Representative images of tau uptake in primary neurons. Scale bar, 50m.",yes
PMC5460083,Figure_2,oa_package/a4/e0/PMC5460083.tar.gz,"['Symptoms included inflammatory cell infiltration into the lung parenchyma and interstitium, thickening of bronchial epithelium, and alveolar expansion (A).', 'Unlike WT mice, IL-1R1-/- mice had less inflammatory cell aggregation in the bronchial walls and alveolar spaces (A).', 'At the late stage of infection (9 dpi), lung inflammation had gradually regressed in the surviving WT mice, with the alveolar structure tending to be complete and a small number of inflammatory cells aggregating around a portion of the lung parenchyma (A).', 'However, both disruption of the alveolar structure and formation of a hyaline membrane were seen in the lungs of dying IL-1R1-/- mice during late infection, with congestion and swelling of the lung interstitium (A).', 'Histological scores demonstrated that IL-1R1-/- mice exhibited less severe lung pathology than that of WT mice during the early infection stage, but significantly more severe lung pathology during the late infection stage (A).', 'The lung wet-to-dry ratios of IL-1R1-/- mice were significantly higher than those of WT mice at 9 dpi, suggesting that the IL-1R1-/- mice suffered more severe lung edema during late infection (B).', 'Analysis of the total protein concentrations and inflammatory cytokine levels in the BALF from infected mice showed that the protein concentration and IL-1 , IL-6, and TNF- levels were significantly higher in WT mice than in IL-1R1-/- mice at 2 dpi (C).', 'At 8 dpi, the levels of IL-6 and TNF- were significantly higher in IL-1R1-/- mice than in WT mice (C).', 'Increased lung inflammation and damage in IL-1R1-/- mice after H1N1 influenza virus infection\nA: Histopathology of H E stained lung sections from WT and IL-1R1 sup -/- /sup mice at 3 and 9 dpi.']",Figure 2 Increased lung inflammation and damage in IL-1R1 mice after H1N1 influenza virus infection,yes
PMC3668897,Figure_4,oa_package/78/99/PMC3668897.tar.gz,['Intranuclear Inclusions.'],"Figure 4 ( ) A pyramidal neuron and an astroglial cell in the frontal cortex containing intranuclear inclusion bodies. Note the pyramidal cell nucleolus adjacent to the intranuclear inclusion and the heterochromatin of the astroglial cell gathered up against the edges of the nucleus. ( ) Granule neuron in the dentate gyrus of the hippocampus with an intranuclear inclusion body. ( ) Granule neuron in the cerebellar granule cell layer with an intranuclear inclusion. Note the heterochromatin is pushed away from the inclusion, forming a dense gathering at the nuclear envelope. Scale bar=50m applies to all plates.",yes
PMC4574454,Figure_3,oa_package/72/63/PMC4574454.tar.gz,[],Fig. 3 Color image of the sentinel nodes under white light source shows visible blue dye,yes
PMC6259469,Figure_3,oa_package/1c/de/PMC6259469.tar.gz,['Steps for protease-K assisted homogenization process.'],Figure 3 Steps for protease-K assisted homogenization process.,yes
PMC9699888,Figure_3,oa_package/b9/c0/PMC9699888.tar.gz,"['When the skin over the depressed area was turned over, there was an area of 6 10 cm in the right parietal bone that was thinned out and was creating a small crater in the calvaria [a].', ':Intraoperative image (a) showing depressed and deformed bone.']","Figure 3: Intraoperative image (a) showing depressed and deformed bone. The outer layer of the excised bone is pigmented and depressed (b), but the inner layer looks intact (c).",yes
PMC5649214,Figure_4,oa_package/3b/dc/PMC5649214.tar.gz,[],"FIGURE 4 Glycolysis stress test after cultivation of neurons in Neurobasal medium. Addition of B27 acutely inhibited glycolytic pathways by decreasing glycolysis as well as the glycolytic capacity (circles), while addition of N2 (triangles) did not further inhibit metabolic function. Compared to GS21 (squares), glycolytic capacity of neurons was substantially decreased with B27 or N2. Respiratory activity was increased after cultivation with GS21 and after its addition to neurons undergoing metabolic flux analysis compared to cultivation with or addition of B27 or N2. Cell mito stress test after cultivation in Neurobasal medium. Maximal respiration was highest after cultivation with GS21 but measurements were performed in the absence of the supplement (open squares). Glycolysis was not affected under these conditions. Open symbols indicate that neurons were cultured in the presence of the respective supplement but that was not present during measurements. Solid symbols indicate that it was also present during measurements. The time points of addition of the injection of the respective reagents after start of the metabolic flux assay are indicated with dotted lines.",yes
PMC9144079,Figure_1,oa_package/f7/40/PMC9144079.tar.gz,"['MDT/MB/WHO alone is effective in most lobomycosis cases (A,B).', 'MDT/MB/WHO associated with surgery can even lead to cure in the disease s disseminated forms (C,D).', 'Thus, this triple drug therapy in combination with surgery not only leads to remission of skin lesions, but also does so at reduced cost ().', 'Institutional Review Board StatementThe Ethical Research Committee of the Acre State Health Department approved the randomized clinical trial [71] whose outcomes are highlighted in and Table 1 (No.', '10157633857497WHO/MDT/MB standard protocol for lobomycosis treatment, SDPA, Acre state, 2020 [71].']","Figure 1 WHO/MDT/MB standard protocol for lobomycosis treatment, SDPA, Acre state, 2020 [ ]. ( ) localized lobomycosis before and ( ) after WHO/MDT/MB treatment for four years. ( ) disseminated lobomycosis before and ( ) after WHO/MDT/MB treatment for four years plus lesion resection twice a week for one year.",yes
PMC10426265,Figure_3,oa_package/e8/cb/PMC10426265.tar.gz,"['071Targeted biopsy tissues from the PCa case with ISUP grade of 2, stained for CD31.']","Figure 3 Targeted biopsy tissues from the PCa case with ISUP grade of 2, stained for CD31. (a) Abundant microvessels in tumor tissues (red box). (b) Tumor cell nuclei (blue) were small and poorly differentiated. The numbers of mature vessels (red arrow) and immature vessels (yellow arrow) were almost equal.",yes
PMC11339124,Figure_5,oa_package/3d/dd/PMC11339124.tar.gz,[],"FIGURE 5 T1w comparison. Shown are the denoised T1w images from the MP2RAGE acquisition from UMB (a), NINDS (b), UPenn (c), MNI (d), and BWH (e). Differences in graywhite contrast, brain coverage, and field inhomogeneity can be seen between sites.",yes
PMC3717027,Figure_8,oa_package/30/76/PMC3717027.tar.gz,['Nuclear localisation of PCOLCE is enriched in OPMD compared to other muscular dystrophies.'],"Figure 8 . Sub-cellular localisation of PCOLCE in DMD, FSHD and DM1 patient muscle. Cryosections were stained for PCOLCE and Dysferlin. Dysferlin shows a sarcolemmal localisation. In DMD and FSHD biopsies there is no clear reduction of PCOLCE at the ECM. In DM1 areas with reduced PCOLCE at the ECM are indicate with arrows. Scale bar equals 10m. . Accumulation of PCOLCE in myonuclei was quantified using overlap in intensity distribution between DAPI (blue) and PCOLCE (red). Plots show two nuclei without enrichment with nuclear PCOLCE (i) or with PCOLCE enrichment (ii). Histogram (iii) shows the quantification of 100 nuclei from FSHD, DMD, DM1 and OPMD muscle biopsies.",yes
PMC4242621,Figure_4,oa_package/ef/ec/PMC4242621.tar.gz,"['Livers from PbNK65-infected mice 10 days after infection contained large clusters of Hz, which were larger and more abundant than those observed in PbANKA (day 8) or PcAS-infected mice (A), corroborating the Hz quantification data (see ).', 'These Hz clusters were mainly observed in sinusoidal macrophages, whereas only limited numbers of Hz-containing neutrophils were observed, which were exclusively found in the lumen of blood vessels (A).', 'g004Hepatic hemozoin is localized in macrophages.', 'Hz crystals (brown) colocalized with F4/80 staining (red), indicating that Hz is mostly situated inside macrophages (B).']",10.1371/journal.pone.0113519.g004,yes
PMC6636106,Figure_5,oa_package/4a/17/PMC6636106.tar.gz,"[' 5) were found in 10/31 of MCNs, and thick walls were detected in 21/31 cases.', '2 mm)Mucinous cystic neoplasm with solid componentCompared with the imaging features of SCNs and MCNs, there were significant differences in the location (P = 0.']",Fig. 5 Mucinous cystic neoplasm with thick wall (3.2mm),yes
PMC5609390,Figure_3,oa_package/4e/5d/PMC5609390.tar.gz,"['7, respectively, (e-g) degraded images with gamma value of 3, 6, and 9, respectivelyCompression ratesAcquired images of each breast cancer HER2 stain were successively compressed in JPEG2000 (JP2) ranging from compression rates of 3200 200 [].', 'Different compression rates.']","Figure 3 Different compression rates. (a) Original image, (b-f) images with a compression rate of 200, 400, 1200, 2200, and 3200, respectively",yes
PMC4456368,Figure_2,oa_package/fb/e2/PMC4456368.tar.gz,"['Time course of VIC activation in DMEMFreshly isolated primary VICs were plated on coverslips in DMEM and fibroblast media separately and fixed over time intervals to assess the level of activation of VICs (A).', 'g002Confocal microscopy of cultured VICs in DMEM and FIB stained with -SMA and doubly stained with SM22 (green) and EDA-fibronectin (red) at 2, 7 and 12 days after isolation (A).', 'VIC myofibroblasts could be dedifferentiated by incubating in fibroblast media for 2 weeks (B) After one week, there was a significant reduction in -SMA, EDA-fibronectin and calponin with a reduction in size and aspect ratio as depicted in Supplementary ).']","FIG. 5. Cytokine expression in the diabetic retina is regulated by pHBSP : IL-10 was decreased in the diabetic rat with scrambled pHBSP relative to the control with scrambled pHBSP (*** < 0.001). The level of , the anti-inflammatory cytokine, was elevated again with pHBSP in the diabetic rat when compared with the scrambled pHBSP (+ < 0.05). : was increased in the diabetic rat, which received 10 g/kg of scrambled pHBSP, relative to the control, which received the scrambled pHBSP (*** < 0.001). This elevated level in the diabetic rat was significantly decreased with the active pHBSP (+++ < 0.001). : IL-6 was increased in the diabetic rat, which received 10 g/kg of scrambled pHBSP, relative to the control, which received the scrambled pHBSP (*** < 0.001). This elevated level in the diabetic rat was significantly decreased with the active pHBSP (+++ < 0.001). : STZ-induced diabetes caused a reduction in levels when compared with control tissue treated with scrambled peptide (*** < 0.001). Treatment with pHBSP caused a reduction in levels both in nondiabetic tissue and diabetic tissue (*** < 0.001; +++ < 0.001). The number of animals in each group was six. Cntl, control; Sc, scrambled pHBSP; and Db, diabetic.",yes
PMC4656239,Figure_2,oa_package/7a/04/PMC4656239.tar.gz,"[' 2).', 'A 31-year-old female: Motor vehicle accident and compression fracture of T12: axial CT (a) and coronal reformats (b) show misplaced bilateral transpedicular screws at L1 abutting the medial cortex of pedicles']",Fig. 2 A 31-year-old female: Motor vehicle accident and compression fracture of T12: axial CT ( ) and coronal reformats ( ) show misplaced bilateral transpedicular screws at L1 abutting the medial cortex of pedicles,yes
PMC7863914,Figure_5,oa_package/29/8f/PMC7863914.tar.gz,"['Patient neutrophils had impaired bacterial killing of Streptococcus pneumoniae S14 ( 5A) and S.', ' aureus SH1000 ( 5B), with defective LPS survival responses ( 5C).', 'In keeping with a dysregulated metabolic status, COPD neutrophils had significantly lower ATP levels at baseline when compared to healthy controls ( 5D) and a reduced glycolytic response to SH1000 (s 5E and 5F).', 'Diminished glycolytic capacity occurred despite preservation of glucose uptake with equivalent tracing of U-13C glucose into glucose-6-phosphate ( 5G) and retained expression of the dominant neutrophil glucose transporter GLUT1 ( S6A) in unstimulated and LPS-stimulated states.', 'Initial baseline phenotyping of peripheral blood neutrophils revealed an impaired ability of COPD neutrophils to increase glycogen levels in response to LPS ( 5H) and to increase transcription of the key gluconeogenic enzyme PEPCK2 ( 5I) and the glycogenic enzyme GBE1 ( 5J).', 'COPD neutrophils displayed significantly lower lactate levels with normoxic culture alone and following LPS stimulation ( 5K).', 'This was associated with a decreased level of lactate in the medium of COPD neutrophils cultured with LPS ( 5L) and reduced levels of the PPP substrates ribose-5-phosphate ( 5M) and sedoheptulose-7-phosphate ( 5N).', 'COPD neutrophils also demonstrated a failure to generate the metabolites required for effective redox buffering capacity (NADH) ( 5O) and pathogen sensing responses (UDP-glucose and UDP-GlcNAc) (s 5P and 5Q) (Jha et al.', 'Finally, tracing of U-13C glutamine into F1,6BP ( 5R) demonstrated that COPD neutrophils have a diminished capacity to utilize glutamine for gluconeogenesis.', ' 5COPD Peripheral Blood Neutrophils Are Unable to Regulate Their Glycogen Synthesis, Resulting in Diminished Intracellular Glycogen Stores, Defective Bacterial Killing, and Survival(A C) Neutrophils from healthy control subjects (HC; open black circle) or patients with COPD (COPD; open blue square) were challenged with either opsonized serotype 14 Streptococcus pneumoniae (S14) (A; n = 7) or Staphylococcus aureus (SH1000) (B; n = 4), at a multiplicity of infection (MOI) of 10 and bacterial killing assessed.']","Figure4 Glycogen Storage Capacity Dictates Neutrophil Function and Survival in Gys1 MRP8-cre Mice and Healthy Human Volunteers Exposed to Altitude-Induced Hypoxia (A and B) Neutrophil glycogen content following 6 (A) or 4h (B) normoxic culture in glucose-deplete media with the glutaminase inhibitor BPTES preventing glutamine breakdown (A; n= 4) and the gluconeogenesis inhibitor MB05032 (B; n= 3). (CE) Assessment of apoptosis rates using flow cytometry (C) and cellular morphology (D and E) following culture in glutamine-deprived (C) or glucose-deplete media (D and E) under conditions of normoxia and hypoxia LPS for 6 (D) and 20h (C). Data shown as mean SEM (C and D) and fold change from DMSO vehicle control. n= 4. (F and G) Neutrophils were challenged with (SH1000) at a multiplicity of infection (MOI) of 10 and bacterial killing assessed by flow cytometry. Data shown as fold change from DMSO (F; n= 7) and untreated (G; n= 3) controls. (H) Glycogen content of neutrophils in the bone marrow, blood, and bronchoalveolar lavage (BAL) of untreated and 24h post-LPS challenge of wild-type mice (WT). n= 3. (I) Glycogen content of circulating and BAL neutrophils of WT and knockout mice. n= 3. (J) Assessment of apoptosis rates using flow cytometry following 24h of culture under standard media conditions. n= 5. (K) challenge of BAL neutrophils with (SH1000) (MOI:10) and bacterial killing assessed by flow cytometry. n= 3. (LN) MRP8-Cre knockout ( KO) and MRP8-Cre WT mice were inoculated with 5 10 CFU of (SH1000) and rectal temperatures (L), total abscess CFU counts (M), and blood neutrophil counts (N) obtained 24h post-subcutaneous infection. (OR) Intracellular glycogen levels (O; n= 6), UGP2 (P; n= 8) and GYS1 (Q; n= 7) relative transcript abundance, and apoptosis rates (R; n= 8) were measured in blood neutrophils isolated from healthy human volunteers at baseline (BL) and 3months post-altitude-induced hypoxia (PA), following culture with LPS and hypoxia. Data represent mean SEM. Statistical significance was determined by paired t tests (A, B, DF, K, P, and Q), two-way ANOVA with Sidaks multiple comparisons test (C, O, and R), one-way ANOVA with Tukeys multiple comparisons test (C, G, and H), and unpaired t tests (I, J, and LN). p< 0.05, p< 0.01, p< 0.005, p< 0.001.",yes
PMC11377372,Figure_4,oa_package/94/d5/PMC11377372.tar.gz,"[' 4A, B, C).', ' 4D, E, F).', '', 'Clustering and annotation of single-cell transcriptomesAfter downloading and cleaning the single-cell data, 86,090 cells were obtained.']","Fig.4 Comparison of immune infiltration between MPLC and SPLC for both mGGN and pGGN. , , comparison of differences in tumor immune infiltration between the pGGN nodules in MPLC and SPLC (assessed using the CIBERSORT algorithm, the MCPcounter algorithm and the ESTIMATE algorithm). , , comparison of differences in tumor immune infiltration between the mGGN nodules in MPLC and SPLC (assessed using the CIBERSORT algorithm, the MCPcounter algorithm and the ESTIMATE algorithm). * indicates <0.05, ** indicates <0.01, *** indicates <0.001, and **** indicates <0.0001.",yes
PMC8752195,Figure_9,oa_package/78/cc/PMC8752195.tar.gz,"['5\n\nAfter blocking blood flow, the bladder should be pinched and elevated manually, and the bladder should be separated from the uterine wall by using an electric cautery device (\n\n).', '\nSeparation of the bladder.']","Fig. 9 Separation of the bladder. If an arterial balloon occlusion catheter is in place, blood flow should be blocked at this point to initiate separation of the bladder. The bladder should be pinched and elevated (), and separated quickly using an electric cautery device. Blood vessels should be ligated as much as possible, and bleeding from the surface should be controlled by applying towel pressure to facilitate quick separation. (Reproduced with permission from Takeda S. Murayama Y. Cesarean hysterectomy. In: Hiramatsu Y, Konishi I, Sakuragi N, Takeda S, eds. Surgery for pregnancy with placenta previa and placenta accrete: Careful preparation and critical management. OGS NOW, No.9. [Japanese]. Tokyo: Medical View; 2012:122133. Copyright Medical View).",yes
PMC6556525,Figure_1,oa_package/38/c0/PMC6556525.tar.gz,"['The left eye fundus had multiple peripheral retinal hemorrhages and a feather-shaped white preretinal membrane in the left eye (A).', 'Handheld optical coherence tomography (OCT) of the left eye was remarkable for a preretinal membrane ().', 'Preoperative fundus photo (Box A) and OCT shows preretinal membrane.', '', 'The left eye fundus exam revealed no evidence of recurrent sub-ILM membrane (B).', 'OCT confirmed the absence of the sub-ILM membrane, epiretinal membrane and macular edema 1 month after the surgery (B).']",Fig. 1 Preoperative fundus photo (Box A) and OCT shows preretinal membrane. Postoperative fundus photo (Box B) shows absence of the sub-ILM membrane one month after the surgery.,yes
PMC9485677,Figure_8,oa_package/89/81/PMC9485677.tar.gz,[],Figure8 ROC curves of the 5 models in the training and validation sets.,yes
PMC6836709,Figure_5,oa_package/7c/3f/PMC6836709.tar.gz,"['xlsx"" id=""d35e1016"" content-type=""local-data""/>.', 'Source Data for Fc = 3.093]). On day 22 after immunization, general motor activity was assessed using the EthoVision XT 13.0 Open Field Activity System (Noldus) ( , heatmap images representing overall motor activity; , distance traveled; , rearing; , velocity; , acceleration) and ( ) Rotarod testing. Footprint analysis ( , stride length; , print length; , sway length; , toe spread) was also performed. ( ) CIA was induced in male DBA/1J mice by bovine type II collagen immunization, and from 29 dpi, mice were treated with WT TIDM and mTIDM peptides (1 mg/kg BW/d) via intraperitoneal injection. Mice ( = 6/group in 2 independent experiments) were scored daily. Repeated-measures, 1-way ANOVA showed that the WT TIDM peptide significantly protected against CIA (F = 4.927 [>Fc = 3.093]). On day 60 after immunization, general motor activity was assessed using the EthoVision system ( , heatmap images representing overall motor activity; , distance traveled; , rearing; , velocity), Rotarod testing ( ), and grip strength testing ( ). Footprint analysis ( , stride length; , print length; , sway length; , toe spread) was also performed. Six mice ( = 6/group) were used in 2 independent experiments. < 0.001 and < 0.05 versus control; < 0.001 and < 0.05 versus EAE or CIA by 2-sample test Data represent the mean SEM.",yes
PMC5155175,Figure_2,oa_package/15/21/PMC5155175.tar.gz,['Goblets per colon (A) and cecum gland (B) counted from cross sections stained with Alcian blue and nuclear fast red (C).'],"Figure 2 Goblets per colon (A) and cecum gland (B) counted from cross sections stained with Alcian blue and nuclear fast red (C). Each point in panels A and B represents a separate gland. Between 3-15 and 7-14 glands (obtained from 8 WT and 9 Nox1-KO mice) were counted from colon and cecum, respectively. Scale bar approximately 0.5 mm. ""a"" indicates a statistically significant difference between the 2 groups.",yes
PMC10747561,Figure_3,oa_package/a2/c0/PMC10747561.tar.gz,"[', inflammatory cells, mainly lymphocytes, infiltrated the epithelium with exfoliated epithelial cells and epithelial degeneration ().', 'Trachea H E staining on dpi 5.']","Figure 3 Trachea H&E staining on dpi 5. ( ): challenge groups AClymphocytic inflammation with epithelial degeneration (see arrows) 20, bar = 100 m. ( ): control groupintact ciliated epithelial cells, 40, bar = 50 m.",yes
PMC6342746,Figure_3,oa_package/b8/ad/PMC6342746.tar.gz,"[' 3), in which no compression is applied.', 'Ultrasound (US) image fused with a previously acquired computed tomography (CT).', 'Interposed colon on the CT image is displaced on US by the strong compression applied by the probeConclusionUltrasound-guided percutaneous intervention for pancreatic diseases is increasingly used and is now part of clinical practice in high-volume centres all over the world.']",Fig. 3 Ultrasound (US) image fused with a previously acquired computed tomography (CT). Target lesion easily is identified and marked () on both sides. Color Doppler confirms the major vessels relationship of the lesion well visualised on the CT on the left. Path of the needle precisely planned ( ). Interposed colon on the CT image is displaced on US by the strong compression applied by the probe,yes
PMC4270229,Figure_8,oa_package/25/0f/PMC4270229.tar.gz,[],"Figure 8. Application of the bleomycin model in evaluating the efficacy of therapeutic treatment with compounds targeting key components of signaling pathways involved in the pathogenesis of pulmonary fibrosis. (A) Lung function measurements for (A) total lung capacity (B) dynamic resistance, (C) dynamic compliance, (D) tissue damping as an index of inhomogeneity in airflow in peripheral airways, and (E) tissue elastance in the peripheral airways were performed in order to investigate the impact of therapeutic treatment with the ALK5 small molecule inhibitor compound: SB525334 (3, 10 mg/kg/day) or vehicle alone, on an established fibrotic response in the bleomycin model. Animals were dosed with compound or vehicle, from day 7 onwards and after the appearance of pathological fibrotic lesions. The pulmonary pathology was assessed by morphometric analysis of PicroSirius red (PSR)stained tissue sections using the Ashcroft scoring system (F), gene transcript levels for collagen 3 1 as determined by quantitative TaqMan PcR (G) and hydroxyproline content (H) in lung tissue. The data are presented as mean SEM for each group ( = 8), with statistical significance (* < 0.05, ** < 0.01, *** < 0.001), as determined using oneway ANOVA.",yes
PMC8057286,Figure_4,oa_package/48/8b/PMC8057286.tar.gz,"['A middle-aged female presented with cough, fever, and body aches on the fourth day of symptoms.']","Figure 4 A middle-aged female presented with cough, fever, and body aches on the fourth day of symptoms. HRCT scan illustrates bilateral areas of ground-glass opacities (black arrowheads), the subpleural atelectatic band in right lower lobe (white arrow), and prominent bronchovascular markings in the left lower lobe (black arrows). HRCT: High-resolution computed tomography.",yes
PMC10542668,Figure_3,oa_package/20/06/PMC10542668.tar.gz,"[' 3).', ' 3).', 'Amplification of ACTB in extracted DNA samples.', 'PCR products (135 bp) were visualized using 1% agarose gel electrophoresisDiscussionViral persistence in human heart tissue is a well-recognized phenomenon.', ' 3).']","Fig. 3 Amplification of ACTB in extracted DNA samples. Homogenized and non-homogenized heart tissues were incubated at 55C in the presence of proteinase K for 1, 2, 4 and 24h. PCR products (135bp) were visualized using 1% agarose gel electrophoresis",yes
PMC5445699,Figure_3,oa_package/67/53/PMC5445699.tar.gz,[],"Fig. (3) Immunohistochemistry analysis of BACE1 expression in brain sections following chronic RF-EMF exposed Tg-5xFAD and WT mice. Arrow indicates BACE negative A plaques. Scale bar: 100m. Western blotting of BACE1 in the hippocampus and cortex extract in Tg-5xFAD and WT mice following RF-EMF exposure. Values are presented as the mean SEM and analyzed by ANOVA (analysis of variance) and Bonferroni post-hoc test (# p < 0.05 . WT-RF(-), * p < 0.05 WT-RF (+), + p < 0.05 Tg-5xFAD-RF (-)).",yes
PMC10192583,Figure_3,oa_package/30/7b/PMC10192583.tar.gz,['The present study hypothesizes that tumor sEV CXCL8 mRNA levels may be used to monitor recurrence risk during remission and monitor tumor senescence ().'],"FIGURE 3 JAK/STAT pathway. An intracellular pathway (JAK/STAT) consists of a class of intracellular molecules (Janus Kinase) that respond to upstream receptor signals and the activated JAK catalyzes tyrosine phosphorylation of the receptor by rapidly recruiting to the receptor and activating it. In addition to binding to the receptor molecule, phosphorylated tyrosine on it is a binding site for signaling molecules, such as SH2, that bind to the receptor and phosphorylate tyrosine, forming a dimer that enters the nucleus, and STAT (signal transducer and activator of transcription). Regulation of the JAK/STAT pathway is mediated by interferon binding and activation of its receptor.",yes
PMC11327311,Figure_7,oa_package/d8/07/PMC11327311.tar.gz,"['7A).', '7B, Supplementary ', '7C), especially regulation of calcium ion transport into cytosol (', '7D).', '7E) into the cerebellar vermis and examined fluorescence signals in slices 3 weeks later (', '7F).', '7F, G).', 'PN-specific deletion of TLR4 reduced cytosolic calcium concentration (cytoCa2+), damaged mitochondria, and disrupted associations between mitochondria and endoplasmic reticulum (ER).', 'A loss-of-function BK channel mutation (BKG354S) was found to cause mitochondrial impairment and progressive cerebellar ataxia in patients [26], so we examined if mitochondria are impaired by PN-specific TLR4 knockout by transmission electron microscopy.', '7H and Supplementary ', '7H and Supplementary ', '7H).']","Fig. 7 PN-specific deletion of TLR4 reduced cytosolic calcium concentration (cytoCa ), damaged mitochondria, and disrupted associations between mitochondria and endoplasmic reticulum (ER). Top KEGG pathways downregulated in TLR4 cerebellum. GSEA visualization of calcium signaling pathways. The top calcium signaling-related GO pathways downregulated in TLR4 cerebellum. GSEA visualization of regulation of calcium ion transport into cytosol. Schematic illustration showing injection of a pAAV encoding the cytoCa indicator jRGECO1a into the cerebellar vermis. Representative confocal images of pAAV-L7-NES-jRGECO1a labeling, indicative of cytoCa , in PNs of a cerebellar sagittal section. The magnification images show the cytoCa signal in soma and dendrite. Decreased MFI of jRGECO1a indicating reduced cytoCa in soma and dendrites of TLR4 PNs. Each point in the histogram represents a mouse. Representative transmission electron images of PNs in four-month-old TLR4 cerebellum showing somal shrinkage, increased cytoplasmic density, increased electron density, nucleolar fragmentation, nucleoplasmic concentration, mitochondrial swelling, and degeneration of mitochondrial double membranes and cristae (red arrow). Mitochondria in TLR4 PNs also showed weakened ERmitochondria tethering (dotted green lines indicate ER in contact with mitochondria, dotted blue lines indicate ER not in contact with mitochondria). Schematic illustration of these results. Scale bar=20m in lower magnification and 5m in higher magnification images ( ), 5m, 500nm, 250nm, 100nm in order of increasing magnification images ( ). The significance threshold was set to an adjusted <0.05 ( ). CMC cardiac muscle contraction (in ); ER endoplasmic reticulum, Mito mitochondria.",yes
PMC4186498,Figure_5,oa_package/6b/98/PMC4186498.tar.gz,"['The pathology was again consistent with the diagnosis of metastatic adenocarcinoma () and immunohistochemical staining of the core biopsy samples revealed a cytokeratin 7 (CK7)-negative/CK20-positive pattern, which is typical of colorectal primary carcinoma.', 'Core biopsy of the mediastinal lymph node showing adenocarcinoma (stain, hematoxylin and eosin; magnification, 40).']","Figure 5 Core biopsy of the mediastinal lymph node showing adenocarcinoma (stain, hematoxylin and eosin; magnification, 40).",yes
PMC6745076,Figure_3,oa_package/22/09/PMC6745076.tar.gz,"[' 3 and Table 3).', 'CSF endo-lysosomal proteins and ubiquitin concentrations in clinical study I.', 'Statistics were calculated using Kruskal-Wallis test with Dunn s test for multiple comparisons and the graphs show Tukey boxplotsThe finding in clinical study I that proteins had lower concentration in PD and no alterations in prodromal AD or AD dementia called for an additional investigation in a clinical cohort.']","Fig. 3 CSF endo-lysosomal proteins and ubiquitin concentrations in clinical study I. Clinical study I included cross-sectional samples from clinically characterized subjects with AD ( =6) and PD ( =10) as well as longitudinal samples from subjects with prodromal AD ( =10) and stable MCI ( =15). A significant decreased concentration of AP2B1_868878 ( <0.05), CTSF_103116 ( < 0.05), LAMP1_357363 ( <0.05), LAMP2_133144 ( <0.05), LAMP2_153161 ( <0.05), LAMP2_281289 ( <0.01), Ubiquitin_1227 ( <0.05), and Ubiquitin_6472 ( <0.05) was identified in PD compared to prodromal AD. Statistics were calculated using Kruskal-Wallis test with Dunns test for multiple comparisons and the graphs show Tukey boxplots",yes
PMC5072139,Figure_1,oa_package/c7/44/PMC5072139.tar.gz,"['The tumor contained calcifications along with heterogeneous cystic lesions measuring 210 154 mm (B).', 'Finally a 35 35 cm mass was totally excised and sent to the lab for pathologic evaluations (A).', '(A) Gross view of the resected tumor showing various tissue types within the mass.', '']",Fig. 1 (A) Gross view of the resected tumor showing various tissue types within the mass. (B) Axial view of the CT scan showing a large retroperitoneal tumor posterior to the pancreas and anterior to the left kidney pushingthe stomach and the pancreas forward and compressing on the small bowel loops. The tumor contained calcifications along with heterogeneous cystic lesions measuring 210154mm.,yes
PMC6689122,Figure_3,oa_package/c5/af/PMC6689122.tar.gz,['\nVideo 1The heterogeneous texture and the mobile nature of the fibroelastoma are illustrated in this video.'],Figure 3 Three-dimensional (3D) TOE reconstruction of an view of the orifice of the LAA and left upper pulmonary vein (LUPV). The fibroelastoma (arrow) is seen to originate from the orifice of the LAA at the Coumadin ridge.,yes
PMC2719355,Figure_8,oa_package/52/48/PMC2719355.tar.gz,"['One possible explanation of the observed attenuation effects is that both rBChE-U and the BSP peptides can induce a shift of A accumulation toward a stabilized A oligomer, as large as 100-mer, if judged by the 1:100 molar ratio of effective inhibition ().']","FIGURE 8. The A aggregation pathway involves a set of mutually dependent reactions in complex equilibria (monomer dimer oligomer fibril). The transition from helical structure to -sheet conformation was studied by CD, oligomerization was followed by cross-linking, and fibril formation was tracked by ThT fluorescence and TEM. These reactions along the A aggregation pathway are differentially affected by rBChE-U, BSP-U, and BSP-K.",yes
PMC7155095,Figure_1,oa_package/4f/31/PMC7155095.tar.gz,['NRP1 expression correlates with VEGFR2/NRP1 complex formation.'],"Figure 1 NRP1 expression correlates with VEGFR2/NRP1 complex formation. (A) Schematic figure of endothelial expressed VEGFR2 (red) and NRP1 (green) expressed either on endothelial or perivascular tumor cells, forming VEGFR2/NRP1 complexes in (left panel) and configurations (right panel). (B) Representative images of RCC patient biopsies immunostained for VEGFR2 (red), CD34 (cyan), and NRP1 (green) and counterstained using Hoechst33342 (blue). Top row shows an overview of merged immunostaining; boxed regions are shown as magnified individual channels below. Left column shows examples of NRP1 expressed by endothelial cells (white open arrows); middle column shows a tumor sample with low NRP1 expression. Right column shows NRP1positive perivascular tumor cells (white arrowheads). Scale bars, 100m (top row) and 40m. (C) PLA for VEGFR2/NRP1 complex formation on sections consecutive to (B), highlighting the relationship between NRP1 expression pattern and complex formation in and . VEGFR2/NRP1 complexes are detected as red punctuates. Blood vessels were stained for CD34 (green) and nuclei counterstained using Hoechst33342 (blue). Left column shows complexes, localized within the endothelium (white open arrows). Middle column shows a tumor lacking VEGFR2/NRP1 complexes. Right column shows complexes, located adjacent to the endothelium (white arrowheads). Scale bars, 40m.",yes
PMC3548646,Figure_2,oa_package/4e/88/PMC3548646.tar.gz,['Foci of ameloblastomatous epithelium in a dental follicle (H.'],Figure 2 Foci of ameloblastomatous epithelium in a dental follicle (H.E.; orig. magn. x25).,yes
PMC6637537,Figure_3,oa_package/46/6c/PMC6637537.tar.gz,"[' 3).', 'Arrows are pointing to site of lesionA referral was made to Otolaryngology- Head and Neck Surgery.']","Fig. 3 CT Head following initial presentation to the emergency department, with axial and coronal views. Arrows are pointing to site of lesion",yes
PMC9564154,Figure_1,oa_package/e5/18/PMC9564154.tar.gz,"['Serum biomarkers can be categorized as protein markers, growth factors and circulating nucleic acid ().', 'Neutrophil-to-Lymphocyte RatioNeutrophil-to-lymphocyte ratio (NLR) is a simple biochemical parameter initially proposed as a marker of inflammatory status and disease-related inflammation () [81].', 'ProteoglycansProteoglycans, a group of highly glycosylated proteins mainly located in cell membranes and the extracellular matrix, are implicated in the modulation of neoplastic progression due to their role in signaling activities that can influence the availability of growth factors involved in stromal environment remodeling, angiogenesis and tissue regeneration () [98].', '04) suggesting a possible role of these genes as future biomarkers in the prediction of HCC response to chemotherapy () [107].', 'Organoid CulturesThe organoids (tumoroids/organoid cultures) derived from primary liver cancers have an enormous potential as prognostic biomarkers in drug screening or resistance detection, due to their characteristic mirroring of parental tumor histology, genetic and transcriptome patterns () [108].', '147935582676Many heterogeneous categories of biomarkers are being investigated as possible prognostic parameters in predicting the therapeutic efficacy and survival in advanced HCC.']","Figure 1 Many heterogeneous categories of biomarkers are being investigated as possible prognostic parameters in predicting the therapeutic efficacy and survival in advanced HCC. AFP: alpha-fetoprotein, PD-L1: programmed cell death-1 ligand, DCP: des--carboxy prothrombin, sBTLA: soluble B and T lymphocyte attenuator, ADAM9: a-disintegrin-and-a-metalloprotease-9, HGF: hepatocyte growth factor, VEGF: vascular endothelial growth factor, PDGF: platelet-derived growth factor, FGF-19: fibroblast growth factor 19, TGF-1: transforming growth factor beta 1, miR: microRNA, hTERT: human telomerase reverse transcriptase.",yes
PMC3745117,Figure_16,oa_package/9d/cf/PMC3745117.tar.gz,[],Figure 16 A case of Crouzon's syndrome operated for fronto-orbital advancement earlier (upper row) underwent mid-face advancement at le fort 1 and 3 levels (middle row). Lower row shows 2 years post-operative appearance,yes
PMC11567857,Figure_4,oa_package/5a/9a/PMC11567857.tar.gz,[],"FIGURE 4 Multiomics analyses suggest that miR33 may regulate microglial migration and immune response. A, Volcano plot visualizing the 882 DEGs identified by bulk RNAsequencing (RNAseq) from cortical tissue between ; (WT) and ; (KO) mice. B, Volcano plot visualizing the 125 DAPs identified by mass spectrometry (MS/MS) from cortical lysate between ; (WT) and ; (KO) mice. C, Pathway Map enrichment analysis was performed with the DEGs (red) and DAPs (blue) identified between ; and ; mice using the MetaCore software. The top 10 Pathway Maps are shown with the vertical red line denoting significant Pathway Maps identified. D, The shared response are the common DEGs and DAPs between RNAseq and MS/MS visualized as a function of their statistic. E, GO enrichment analysis was performed with the shared response common DEGs and DAPs using the MetaCore software. The top 10 GO Processes are shown with the vertical red line denoting significant GO Processes identified. DAPs, differentially abundant proteins; DEGs, differentially expressed genes; GO, Gene Ontology; KO, knock out; WT, wild type.",yes
PMC8563784,Figure_6,oa_package/20/a9/PMC8563784.tar.gz,[],"Figure6 Late-onset of berberine adjunctive therapy against infection in C57BL/6 mice. Animals were infected with (100CFU) aerosol inhalation. After 3 weeks of infection, mice were either left untreated or treated with berberine (1 mg/ml) or rifampin/isoniazid (0.1 mg/ml) and rifampin/isoniazid in combination with berberine in drinking water for 4 weeks as shown in the layout. Mycobacterial burdens in the lung and spleen. Representative lung section for pathology. Quantification of free alveolar air spaces in the lungs at 4 weeks post-treatment. Data is shown representative of two experiments (n = 5 per group) and the line denotes mean value, analysed by Brown-Forsythe and Welch ANOVA test with Tamhane T2 test defining differences in all groups as significant *P 0.05; ***P 0.001. Asterisks without the line below show the significance compared to the untreated group.",yes
PMC3177420,Figure_4,oa_package/5a/e5/PMC3177420.tar.gz,['Sonographic image of a healthy infant shows the transverse portion of the duodenum positioned normally between superior mesenteric artery (SMA) and abdominal aorta.'],Figure 4 Sonographic image of a healthy infant shows the transverse portion of the duodenum positioned normally between superior mesenteric artery (SMA) and abdominal aorta.,yes
PMC4913180,Figure_2,oa_package/f1/58/PMC4913180.tar.gz,"['Magnetic resonance imaging (MRI) showed a well-defined lesion in the left sub-diaphragmatic area attached to the lower face of the diaphragm, which had compressed the spleen (a d).', '']",Fig. 1 (a) An axial enhanced CT image shows that a well-defined mildly hypodense solid mass contains several foci of faint calcifications. (b) A thin band formation between the mass and left lobe of the liver is clearly seen in a coronal reformatted CT image.,yes
PMC3214038,Figure_6,oa_package/55/59/PMC3214038.tar.gz,"['01) generated 2D (A) and 3D (', 'Region-specific metabolic activity differed between groups in both age groups, bilateral hypometabolism (depicted in blue in B) was already uncovered in PLB1Triple cf.', 'wild-type mice at 5 months (Ai and Bi), particularly in the occipital and parietal cortices, while ventral elements, encompassing the basal forebrain, striatum, thalamus and pons were metabolically hyperactive (', 'Though a precise identification of anatomical details is difficult, structural information obtained from the Digimouse atlas and alignments with Paxino-Watson coordinates indicated that hypometabolic cortical regions extended with age to reach the rostral tip of the prefrontal region as well as the cerebellum in 17 months old PLB1Triple mice (blue areas in B).', 'Furthermore, areas of hypermetabolism in transgenic mice also expanded, and stretched from the posterior end of the olfactory bulb, via ventral orbital cortex, continuing throughout the basal forebrain nuclei, basal ganglia including caudate and putamen, ventral thalamus and hypothalamus, pons and continuing into the brain stem (red areas in B).', 'g006Brain metabolism: FDG PET/CT phenotype of PLB1 mice.']",10.1371/journal.pone.0027068.g006,yes
PMC8287342,Figure_4,oa_package/f5/6a/PMC8287342.tar.gz,"['.', '1177_2050313X211027094-fig4"" position=""float""/>As a cavitary defect was generated, two-stage wound closure was designed.']","Figure 4. (a) The histologic specimens showed the appearance of into the cytoplasm of macrophages (arrow),hematoxylin and eosin staining (a) (400), and periodic acidSchiff (PAS)staining (inset) (400) (b).",yes
PMC11693861,Figure_2,oa_package/37/61/PMC11693861.tar.gz,"['As shown in\nA, a gentle dilution curve confirmed that the amount of sequencing data was saturated.', '\n\nGut microbiota dysbiosis in mice induced by arsenic(A) Accumulation curves and alpha diversity.', '\nPrincipal component analysis (PCoA) of the gut flora in the mice from different arsenic supplementation groups demonstrated that distinct separations were observed in the PCoA among the NC, L-As, M-As, and H-As groups, indicating a significant change in the gut microbiota composition induced by arsenic (\nB).', 'The relative abundances of the top 30 intestinal microbes were shown in\nC.', 'At the genus level (\nD)\n, the gut flora species among the groups were not obviously the same, arsenic increased the abundances of\nEscherichia-Shigella,\nBacteroides ,\nBlautia, and\nParasutterella in the mice.', 'E showed the relative abundance of altered gut bacterial genera and the indicators.', 'Arsenic induced changes in membrane transport, amino acid metabolism, lipid metabolism, energy metabolism, and cellular processes and signaling (\nF).', '05) (\nG).']",,yes
PMC11351481,Figure_3,oa_package/ee/19/PMC11351481.tar.gz,"['Our results showed that treatment with RvD5 restored catalase activity (A).', 'Exposure to UVB irradiation increased superoxide anion production comparable with non-irradiated control (B).', 'UVB irradiation increased the formation of LOOH and treatment with RvD5 30 pg/mouse reduced this production (C).', 'RvD5 inhibited UVB irradiation-induced decrease of skin catalase activity, and induction of superoxide anion production and lipid peroxidation.']","Figure 3 RvD5 inhibited UVB irradiation-induced decrease of skin catalase activity, and induction of superoxide anion production and lipid peroxidation. Protocol was followed as depicted in A to investigate ( ) catalase activity, ( ) superoxide anion production, and ( ) lipid peroxidation end-product LOOH. Bars are representative of two separate experiments and represent means SEM of 6 mice per group per experiment. Statistical analysis was performed by one-way ANOVA followed by Tukeys test [* < 0.05 compared to the non-irradiated control group; < 0.05 compared to the irradiated control group (vehicle)].",yes
PMC6220320,Figure_6,oa_package/ab/d4/PMC6220320.tar.gz,['Vesicle cycling in MOG/CFA injected mice (injected with commercial MOG/CFA suspensions; Hooke kit) at day 9 after injection is strongly reduced in comparison to control mice\nA CRetinal slices from MOG/CFA injected and CFA injected SypHy mice were stimulated by applying a 25 mM K+ containing depolarization solution for 1 min (A).'],"Figure 5 Optic nerve parameters are unaltered in early (9days after injection) Data information: Scale bars: 1m (A, B, E, F). Error bars are SEM; statistical test: MannWhitney test (Origin Pro).",yes
PMC5634503,Figure_4,oa_package/91/a4/PMC5634503.tar.gz,"[' 4).', ' -Syn impairs ferritinophagy following light-induced photoreceptor damage: (A) Western blot image demonstrating expression of ferritin, LC3, -syn and -actin in retinal lysates of light-exposed and control -syn+/+ and -syn / mice.', '\nProbing for ferritin revealed significantly less expression in -syn / samples relative to -syn+/+ controls as noted in ', ' 4A, lanes 1 2 vs.', ' 4B).', ' 4A, lanes 1 2; ', ' 4B).', ' 4A, lanes 3 4; ', ' 4B).']","Figure 4 -Syn impairs ferritinophagy following light-induced photoreceptor damage: ( ) Western blot image demonstrating expression of ferritin, LC3, -syn and -actin in retinal lysates of light-exposed and control -syn and -syn mice. ( ) Quantification by densitometry after normalization with -actin. n=3, Values are meanSEM of the indicated . ** <0.01, # <0.05). Asterisk (*) indicates comparison of untreated -syn with untreated -syn samples, and hash (#) indicates comparison of light-exposed -syn with untreated -syn controls.",yes
PMC6667607,Figure_18,oa_package/74/a4/PMC6667607.tar.gz,[],"Fig. 18 Os intermetatarseum. A 36-year-old man, investigated for pain in the Achilles. Radiographs demonstrate a spur projection in the dorsum of the foot, overlying the base of the metatarsal (not shown). Coronal FSE T1 demonstrates how this articulates with the base of the second metatarsal, with a synchondrosis (white arrowhead). Axial three-dimensional gradient echo water selective/fluid (WATSf) demonstrates this exostosis arises from the base of the first metatarsal, and extends over the joint with the base of the second metatarsal (white arrow). These ossicles rarely represent a cause or pathology, unless compression irritates the superficial and deep peroneal nerves",yes
PMC5977133,Figure_2,oa_package/b5/83/PMC5977133.tar.gz,"['Patients may present with acute bleeding or with bleeding years after the initial placement () [26].', 'Delayed hepatic artery bleeding can occur secondary to PTBD stent erosion into the hepatic artery (see ).', 'Embolization is more commonly performed, most often with coils (see and ).', '71-year-old female with PTBD erosion into hepatic artery and subsequent massive upper gastrointestinal bleed.']","Figure 2 71-year-old female with PTBD erosion into hepatic artery and subsequent massive upper gastrointestinal bleed. Patient had a PTBD in place for 7 years following a common bile duct (CBD) injury sustained during cholecystectomy with subsequent hepaticojejunostomy and anastomotic narrowing. This stricture failed benign biliary stricture protocol dilation. She presented to the emergency department with hematemesis, hematochezia and hypotension. ( ) Initial angiogram showed extravasation from the hepatic artery (black arrows) and into the small bowel (dashed black arrows). ( ) After coils (white arrows) were placed distally and proximally, repeat hepatic artery angiogram showed cessation of extravasation. ( ) During a routine biliary tube exchange 7 months later, some of the coils (curved white arrows) were noted to have migrated into the bile duct and along the biliary drainage catheter. ( ) These coils were percutaneously removed using a snare.",yes
PMC4832891,Figure_2,oa_package/a3/88/PMC4832891.tar.gz,"['81% (269) cases [Table 2 and ].', '(d) Cut surface showing uterine leiomyoma with secondary change and lipomatous areaTable 2Histopathological within leiomyomas(a) H and E histopathology section showing leiomyoma with hyalinization.']",Figure 2 (a) H and E histopathology section showing leiomyoma with hyalinization. (b) Calcification (c) Cystic change (d) Red degeneration,yes
PMC3716220,Figure_5,oa_package/c1/2f/PMC3716220.tar.gz,"['Echo, Pre-systolic.']","Fig. 5 Echo, Pre-systolic.",yes
PMC5705684,Figure_6,oa_package/40/4f/PMC5705684.tar.gz,"[' 6a,b).', ' 6c, top panel graph).', ' 6c, lower panel).', ' 6c graph).', ' 6d, top panel graph).', ' 6d graph).', ' 6d, lower panel).', ' 6d).', 'Biochemical profile of -Synuclein in PFF-injected animals.', '\nPFF injected mice exhibit different innate immune cell responses in the CNSIt is possible that differences in the phosphorylation state of fibrils may lead to differences in their uptake or clearance by immune cells, such as macrophages and/or microglia18.']","Figure 6 Biochemical profile of -Synuclein in PFF-injected animals. ( ) Midbrain Triton-X soluble samples of injected animals showed no difference in -Synuclein levels between the ipsi-and contralateral side in all fibrilar types and the control PBS- injected animals (-Synuclein monomer is shown in cropped gel/blot). GAPDH was used as a loading control (cropped gel/blot is shown) (n=57 brains/group). Similar in ( ) no differences were found for the Triton-X soluble fraction in the area of the cortex (-Synuclein monomer is shown in cropped gel/blot) (n=4 brains/group). ( ) P- and wt-PFF injections resulted in a shift of the SDS-soluble -Synuclein in higher molecular weight species ipsilaterally in the midbrain. These SDS-soluble -Synuclein fraction was significantly enriched in the P-PFF injected side compared to the wt-PFF treatment (n=5 brains/group). High molecular weight species in both treatments were also positive for the phospho Ser 129 -Synuclein antibody. No difference was observed in the SDS-soluble -Synuclein monomer levels between the ipsi- and the contralateral side for the P-, wt-, S129A-PFF and the PBS-injected animals (-Synuclein monomer is shown in cropped gel/blot) (n-67 animals/group). -tubulin was used as a loading control (cropped gel/blot is shown). ( ) Immunoblot for the SDS-soluble fraction extracted from the cortex of injected animals showed that -Synuclein SDS-soluble high molecular weight species are formed readily in P-PFF-injected animals in both sides compared to the wt treatment. Densitomentry of the ipsilateral -Synuclein levels confirmed the significant difference between the treatments (n=5 animals/group). -Synuclein null mice (/) injected with P-PFF did not show any positive signal with the Syn-1 antibody. The observed -Synuclein species were phosphorylated in nature as seen following immunoblotting with the phospho Ser 129 antibody. No difference was observed in the SDS-soluble -Synuclein monomer levels between the ipsi- and the contralateral side for all types of injected animals (-Synuclein monomer is shown in cropped gel/blot) (n=56 animals/group). -tubulin was used as a loading control (cropped gel/blot is shown). Data represent mean valuesSEM. Differences were estimated using one-way ANOVA followed by Tukeys post-hoc test and paired two-tailed Students t-test. ( ) p=0,0037 ( ) p=0,0053.",yes
PMC5538435,Figure_4,oa_package/63/d6/PMC5538435.tar.gz,"['We identified hundreds of misregulated CE events (A) such as the abnormal inclusion of Bin1 exon 7 (', 'Misregulated CEs were validated using RT PCR (C; Supplemental Table S5) were identified as being misspliced in P0 double-knockout and CDM muscle, with many showing concordant misregulation (E).', '59 in CDM and double-knockout muscle, respectively (F).', 'Although double-knockout mice present with CDM phenotypes and spliceopathy, combined loss of MBNL1 and MBNL2 was insufficient to model the extensive gene expression changes occurring in CDM (G).']","Figure 4. Congenital spliceopathy in double-knockout mice. ( ) Scatter plot comparing CE between P0 wild-type (WT) and double-knockout (DKO) muscle. Significant events are highlighted in orange. ( ) RNA-seq read coverage across exon 7 in wild-type and double-knockout muscle. ( ) Representative RTPCR for exon 7 in P0 quadriceps muscle ( ) and quantification based on replicates ( ). (***) < 0.001, ANOVA. ( ) Total spliceopathy (mean ||) based on 10 CEs in (2KO), ; ; ; (1KO), and double-knockout muscle compared with wild-type controls. (****) < 0.0001, ANOVA. ( ) Scatter plot of values for double-knockout and CDM orthologous CEs. Events misspliced in double-knockout muscle that correlate with CDM are highlighted in orange. The theoretical perfect correlation (dashed gray line) is also shown. ( ) Quantification of exon 19 in CDM and double-knockout muscle. (***) Monotonicity -score >2.0. ( ) Pie chart of total gene expression changes identified in CDM and double-knockout muscle.",yes
PMC2765174,Figure_3,oa_package/3f/36/PMC2765174.tar.gz,"['[78] In it can be seen that the DCE-MRI of a patient with heterogeneous breast tissue and known lobular carcinoma better depicts the extent of disease as compared to the mammogram.', ' (A,B)Mediolateral oblique projection mammogram (A) and fat-saturated axial T1W postcontrast (B) images.']","Figure 3 (A,B) Mediolateral oblique projection mammogram (A) and fat-saturated axial T1W postcontrast (B) images. In this 60-year-old woman who desired breast conservation, DCE-MRI not only demonstrated extensive disease laterally (arrow in B) at the site of the mammographic finding (arrow in A) but also in the medial aspect of the breast (arrowhead in B). The medial breast was biopsied under MRI guidance and there proved to be an additional focus of lobular carcinoma. The surgical management in this case was mastectomy with breast reconstruction",yes
PMC11328984,Figure_1,oa_package/09/66/PMC11328984.tar.gz,['The pelvic inlet angle was measured on the midsagittal reconstruction (top) as shown by the red and green lines.'],"Fig. 1 The pelvic inlet angle was measured on the midsagittal reconstruction (top) as shown by the red and green lines. The gray line (far right) is parallel to the CT table, and the green forms a 90 angle with the gray line. The red line on the axial section (bottom) shows the level where the angle was measured. S = superior, I = inferior, and IA = inferior anterior.",yes
PMC5031259,Figure_4,oa_package/1d/e4/PMC5031259.tar.gz,"[' 4a and Additional file 10: Table S7).', ' 4b).', 'Inhibition of mTOR corrects translational but not transcriptional dysfunction.', 'f Molecular mechanisms of the neuropathology of TSC and new treatment options to be consideredIn accordance with our previous results, these transcripts were essentially all implicated in protein synthesis and mostly coding for ribosomal proteins.', ' 4c and Additional file 11: Table S8).', ' 4d).', ' 4e).', ' 4f).', ' 4).', ' 4b).']","Fig. 4 Inhibition of mTOR corrects translational but not transcriptional dysfunction. Number of upregulated ( ) or downregulated ( ) genes at the level of translation or transcription in cells treated with rapamycin or AZD-8055 as compared to vehicle-treated cells. for genes that change in gene expression in opposite directions by loss ( ) and by compound treatments ( , ), respectively. Overlap highlights how many genes deregulated in TSC2 depleted cells (upregulated on , downregulated on ) can be reset to control levels by inhibitor treatment. Thresholds of differentially expressed gene calling: abs(logFC) >= 0.5, counts per million no less than 1, and FDR < 0.05. of translational efficiency for transcripts of ribosomal proteins in control cells and -deficient cell line (n = 2) after six weeks of differentiation. Vehicle (DMSO)-treated samples reveal the induced TE in depleted cell lines that is largely reversed after treatment with either rapamycin or AZD-8055. displaying fold changes in transcription, translation, and total protein output for genes associated with proteins synthesis, angiogenesis, and inflammation in control and -deficient cell lines. While different levels of translation of protein synthesis factors between vehicle-treated control and mutant cells are balanced out by mTOR inhibitor treatment, the different transcriptional and protein output for angiogenesis and inflammation genes remains unchanged. Values are log2-transformed and normalized to controls. Statistical significance was determined by a two-tailed paired t-test, *** < 110 . for STAT3 or its phosphorylated form (p-STAT3) from neural cultures after six weeks of differentiation; cultured in the presence (+) or absence () of growth factors or rapamycin for the last 12 h before harvest. Elevated phosphorylation even in the presence of rapamycin indicates continued increased pathway activity. Molecular mechanisms of the neuropathology of TSC and new treatment options to be considered",yes
PMC9569960,Figure_10,oa_package/5f/c3/PMC9569960.tar.gz,[],"Figure 10 Insoluble tau levels are highest for cells co-expressing S262D and GSK3. ( ) CHO cells transfected with the tau S262A or S262D variant with or without co-transfection of GSK3 were lysed and the soluble portion of cell lysates were immunoblotted with anti-total tau antibody, Tau-5, and endogenous control protein, -actin, and a representative Western is shown. ( ) The ratio of Tau-5 to -actin was analyzed for the different cell lysates. Total protein loaded was equivalent, though similar -actin levels were present in each sample. Relative soluble tau was determined by quantifying Tau5+ band intensity relative to -actin to quantify the soluble tau content (* represents < 0.05 between two different means, = 3, with error shown as the SEM). ( ) Crude cell lysates from different groups were sequentially extracted by RAB, RIPA, and 70% FA, as indicated, and evaluated by total tau ELISA assay. A significant decrease was observed in soluble tau levels in RAB and RIPA fractions and an increase in the 70% FA fraction for cell lysates from S262D with GSK3 co-expression as compared to the other three cell lysates. Cell lysates for tau S262A without (unshaded) or with (light gray) co-transfection of GSK3 or S262D variant without (medium gray) or with (dark gray) co-transfection of GSK3 (* represents < 0.05 between means, = 3, with error shown as the SEM; ns = not significant).",yes
PMC3193630,Figure_13,oa_package/6b/a2/PMC3193630.tar.gz,[],Figure 13 Semioblique lateral view. The central beam is centred on the web space between the thumb and the index finger,yes
PMC11035018,Figure_3,oa_package/d9/73/PMC11035018.tar.gz,"['Upon follow-up 4 months later, the patient s MCCs exhibited a significant decrease in size with a remaining nonraised area of postinflammatory hyperpigmentation, and pembrolizumab therapy was restarted at this time ().', 'Merkel cell carcinoma of scalp postradiation therapy on 4-month follow-up.']",Fig 3 Merkel cell carcinoma of scalp postradiation therapy on 4-month follow-up.,yes
PMC9505176,Figure_4,oa_package/d9/d5/PMC9505176.tar.gz,"['These procedures were performed on both the extrajunctional (a d) and junctional (e h) regions of the sarcolemma.', 'Importantly, with the complete inhibition of the Na,K-ATPase activity by 500 M ouabain, the same RMP (~ 61 mV) was established in all experimental groups (a h).', 'Measurement of the resting membrane potential (RMP) and estimation of the electrogenic contribution of 2 and 1 Na,K-ATPase isozymes with the sequential addition of 1 M and 500 M ouabain to the bath solution (indicated by arrows).']","Figure 4 Measurement of the resting membrane potential (RMP) and estimation of the electrogenic contribution of 2 and 1 Na,K-ATPase isozymes with the sequential addition of 1 M and 500 M ouabain to the bath solution (indicated by arrows). Measurements were performed in the extrajunctional ( ) and junctional ( ) regions of the sarcolemma of diaphragm muscles. Rats were subjected to 6 days of injections of either 1 mL sterile 0.9% NaCl (vehicle) or ouabain (1 g/kg body weight) dissolved in 1 mL sterile 0.9% NaCl. ( , ) Control group, where rats were subjected to injections of vehicle (0.9% NaCl). ( , ) OUA group, where rats were subjected to injections of ouabain. ( , ) IR group, where rats were subjected to vehicle (0.9% NaCl) and exposed to a single total-body X-ray irradiation (10 Gy). ( , ) OUA + IR group, where rats were chronically treated with ouabain and exposed to IR. Each data point corresponds to the averaged RMP measured in at least 100 muscle fibers of 67 diaphragm muscles.",yes
PMC4797513,Figure_2,oa_package/bd/28/PMC4797513.tar.gz,"['RESULTSEvaluation of rat carotid artery balloon injury modelAfter balloon injury, the injured left CCA of rats showed Evans blue staining for denuded endothelium, with the uninjured right CCA not stained [].', 'Evaluation of vascular balloon injury model.']","Figure 2 Evaluation of vascular balloon injury model. Normal blood vessels (a and c) and injured blood vessels (b and d) (a, b: Evans blue staining, original magnification, 15; c, d: Hematoxylin-eosin staining, original magnification, 200).",yes
PMC2630984,Figure_3,oa_package/3c/ff/PMC2630984.tar.gz,['Validation of G72 primer sets using real-time RT-PCR.'],"Figure 3 . (A) Primer sets G72 #1, #2, #3, #4 and #5, which were designed within the most common G72 exons (see table and Fig. ) were validated using human genomic DNA. Light grey: primer sequence within exon 4. Dark grey: primer sequence within exon 7. Hatched: primer sequence within exon 2. All primer sets gave a strong signal using human genomic DNA (A) and no signal using rat genomic DNA (not shown). (B) Primers sets within exon 2 (hatched) or exon 4 (light grey) readily detected a signal in cDNA that was isolated from HEK-293 cells expressing a His-tagged G72 cDNA, but not in mock-transfected cells (values within range of empty controls). The exon 7 reverse primer annealing sequence was not contained in our G72 cDNA construct and did thus not detect His-G72 (not shown).",yes
PMC6607360,Figure_7,oa_package/e2/1c/PMC6607360.tar.gz,"['In the current study, only one such patient was diagnosed with cocaine-related ATL ().', 'A 44 year old male presented with an altered and decreased level of consciousness for 2 3 days following a reported large amount of crack cocaine inhalation, which caused ATL.', '4.']","Fig. 7 A 44year old male presented with an altered and decreased level of consciousness for 23 days following a reported large amount of crack cocaine inhalation, which caused ATL. An initial MRI demostrated bilateral, symmetric reduced diffusion throughout the PVWM ( ) on DWI MRI ( ) and ADC map ( ), also with PVWM hyperintensity on FLAIR ( ). Four months later, the areas of reduced diffusion within the PVWM evolved into predominantly T2 shine-through (i.e. bright on ADC), as shown on DWI MRI ( ), ADC map ( ), and FLAIR ( ).",yes
PMC4582643,Figure_2,oa_package/d8/a8/PMC4582643.tar.gz,"[' 2a).', '2b and representative images ', ' 2c).', '', '05)Further evidence of cardiac remodeling in the B6 HFD mice comes from additional echocardiography morphometric characteristics.', ' 2d, e; Table 1).']","Fig.2 The hearts from HFD B6 mice display morphologic pathologies. The echocardiography determined left ventricular (LV) mass normalized to tibia length is larger in the HFD B6 mice when compared to the CD B6 mice ( =0.015, N>8). Similarly, the immunofluorescent determined cell size is larger in the HFD B6 mice when compared to the three other mouse groups (B6 CD versus B6 HFD, =0.025, B6 HFD versus MRL HFD =0.032, N>3). Representative cell sizes visualized by -sarcoglycan staining. , Echocardiography also revealed increases in left ventricle anterior wall during diastole and systole in the HFD B6 hearts compared to the CD B6 hearts (N>8, indicate <0.05)",yes
PMC3185270,Figure_3,oa_package/19/a0/PMC3185270.tar.gz,"['002""/>ESD specimens: (a) and (b) macroscopic and microscopic views of the resected specimen; (c) microscopic view of the resected mucosal and submucosal layer and (d) microscopic view of the layer beneath the submucosal dissection demonstrating with the muscular integrity that there were no perforations.']",Figure 3 ESD specimens: (a) and (b) macroscopic and microscopic views of the resected specimen; (c) microscopic view of the resected mucosal and submucosal layer and (d) microscopic view of the layer beneath the submucosal dissection demonstrating with the muscular integrity that there were no perforations.,yes
PMC10474779,Figure_4,oa_package/0e/8e/PMC10474779.tar.gz,"[' 4A, B) and in particular, there was a significant reduction in DBH in both the DH and VH of EAE animals relative to the na ve mice (DH: p 0.', ' 4C), which was reversed in the lumbar spinal cord DH by LC activation (p 0.', ' 4C).', ' 4E).', ' 4G).', 'The effect of early chronic chemogenetic LC activation on noradrenergic projections.', 'Scale bars: A, B, D, F, 100 mThe behavioral effects of rM3D(Gs)-DREADDpeak-mediated LC activationThe effect of chemogenetic LC activation was explored from the peak of motor symptoms (~ 17 dpi), when animals exhibited robust hind limb paralysis (']","Fig. 4 The effect of early chronic chemogenetic LC activation on noradrenergic projections. Representative images showing DBH-positive fibers in the dorsal and ventral horns (DH, VH) of the lumbar spinal cord, PL/IL and M2/M1 cortices, and , , the quantification of DBH immunoreactivity (IR) in arbitrary units (AU). The data represent the mean+SEM and each point corresponds to an individual mouse ( =45 per group). #p<0.05, ###p<0.001 EAE versus nave; * <0.05, ** <0.01, EAE-rM3D versus EAE (Additional file : Table S5). Scale bars: , , , , 100m",yes
PMC6149231,Figure_2,oa_package/99/61/PMC6149231.tar.gz,[':(a) A completely resected large osteochondroma measuring 17 17 cm2 with ribs and the left upper lobe.'],Figure 2: ( ) A completely resected large osteochondroma measuring 17 17 cm with ribs and the left upper lobe. ( ) Low power view of cartilaginous cap with chondrocytes are arranged in an orderly fashion.,yes
PMC8295117,Figure_17,oa_package/45/51/PMC8295117.tar.gz,[],"Fig. 17 Angiocentric glioma. The tumor (arrows) is hyperintense on T1-weighted ( ), T2-weighted ( ), and T2 FLAIR ( ) images, without appreciable enhancement on the post-contrast T1-weighted image ( ). Also, there is no diffusion restriction as seen on the diffusion-weighted image ( ) and ADC map ( )",yes
PMC3177420,Figure_3,oa_package/5a/e5/PMC3177420.tar.gz,['Sonographic image of a healthy infant shows the retroperitoneum in a transverse plane.'],Figure 3 Sonographic image of a healthy infant shows the retroperitoneum in a transverse plane.,yes
PMC9506680,Figure_5,oa_package/b3/b9/PMC9506680.tar.gz,[],Figure 5 Identification of ventral dural defect once intradural hematoma evacuated,yes
PMC3716220,Figure_4,oa_package/c1/2f/PMC3716220.tar.gz,[],"Fig. 4 Echocardiogram, pre-diastolic.",yes
PMC9871613,Figure_3,oa_package/9c/c0/PMC9871613.tar.gz,"['On the day after sclerotherapy, post-interventional swelling was observed in all patients as expected (pre- and post-treatment examples shown in ).']","FIGURE 3 Pre-procedural images of a 2-year-old girl who presented to the VA clinic with left cheek swelling due to a VM of the left cheek and face with involvement of the lateral border of the left eye. Post-interventional swelling occurred in this patient as expected. Pre-procedural images of a 3-year-old girl with a VM of the right cheek, the patient underwent prior traditional medicine treatment leaving extensive scars on the right cheek. Post-interventional swelling occurred as expected in this patient. Pre- and intra-procedural images of a 2-year-old girl with a VM on the upper lip extending up to the right nostril. After the procedure a swelling occurred, and a slight dark discoloration demarcated on the lip. The small necrosis healed quickly and the proportion below the right nostril already shrunk significantly. All patients need further treatment sessions and close follow-up. VA = vascular anomalies.",yes
PMC7552251,Figure_4,oa_package/aa/cd/PMC7552251.tar.gz,"['Horses with Consensus Change of Some KindFor the 54 horses where the radiologists agreed there was at least one radiographic nasal bone change, the radiologists agreed about the presence or absence of bone deposition in n = 47 (87%; see ), the presence or absence of bone thinning in n = 45 (83%; see ), the presence or absence of loss of bone homogeneity in n = 43 (80%), and the presence or absence of soft tissue swelling in n = 49 (91%).', 'Radiographs showing the nasal bones of a horse in which radiologists (n = 2) agreed there was bone deposition that was: (a) typical of affected horses and (b) moderate.']",Figure 4 Radiographs showing the nasal bones of a horse in which radiologists ( = 2) agreed there was bone deposition that was: ( ) typical of affected horses and ( ) moderate.,yes
PMC4682951,Figure_1,oa_package/72/68/PMC4682951.tar.gz,"['tuberculosis\nIn this study, we examined the bacterial burdens in the lung during early (by 14 days post-infection) and late infection (14 112 days post-infection) according to Mtb virulence ().', '01), respectively (A).', 'The CFU values of Mtb K and H37Rv approached similar levels at 112 days post-infection (B).', 'g001Comparative growth profiles of tested Mtb strains in the lungs during a 112 day-infection.', 'In contrast, Mtb H37Ra was the least virulent strain and did not reach optimal growth; its bacterial burden decreased after 28 days post-infection ( and ).', 'Clearly, progressive pulmonary TB is not a result of increasing numbers of viable bacilli in mice but rather a result of an excessive host response to virulent Mtb infection ( and ).']",10.1371/journal.pone.0145234.g001,yes
PMC9484175,Figure_3,oa_package/72/da/PMC9484175.tar.gz,"['3).', 'Microscopic aspects of HCC, NOS.', 'k HCC, NOS associated with calcified bilharzial ova (arrows) (IHC, 100 )Clinical factors affecting HCV-related HCCRegarding age, a significant association was found only between a young age ( 40 years) and a high serum AFP level (p = 0.']","Fig. 3 Microscopic aspects of HCC, NOS. A well differentiated HCC showed thin trabaecule (IHC, 100). A moderately differentiated HCC showed wide acini filled with eosinophilic to bile secretion (IHC, 100). A moderately differentiated HCC showed mixed acinar and trabecular pattern (IHC, 100). A poorly differentiated HCC showed solid pattern with marked nuclear atypia (IHC, 100). HCC, NOS with mild intra-tumoral lymphocytes (IHC, 100). HCC, NOS with hemangiopericytoma like pattern (IHC, 100). HCC, NOS with prominent osteoclast like giant cells (IHC, 100). HCC with prominent lymphovascular invasion (arrows) (IHC, 100). HCC with bile duct invasion with attached tumor emboli to the epithelial cells (arrows) (IHC, 100). HCC with perineural invasion (arrows) (IHC, 100). HCC, NOS associated with calcified bilharzial ova (arrows) (IHC, 100)",yes
PMC11289473,Figure_8,oa_package/97/30/PMC11289473.tar.gz,"[' 8A).', ' 8A).', ' 8B and C).', ' 8D), while actively confirming the labels of these new glomeruli, as determined by our pathologist.', 'Glomerular sub-classification using HistoLens via active collaboration between HistoLens and an expert pathologist.', 'With a larger set of labeled glomeruli, we designed and trained a simple multilayer perceptron in HistoLens using all hand-engineered features and achieved 0.', ' 8E)29s.', ' 8F, which depicts the histopathological differences between respective glomeruli.']",Figure 8 Glomerular sub-classification using via active collaboration between and an expert pathologist. ( ) Initial feature selection and manual annotation of selected glomeruli. ( ) Feature visualization showing regions of image included in calculating feature value. ( ) Screenshot of interactive session between pathologist and first author. ( ) Using relative positioning of labeled examples for bulk annotation. ( ) Performance of multilayer perceptron classifier on validation set of labeled images. The trained model was applied to unlabeled glomeruli for labeling. ( ) Merging disease and nodularity labels to examine differential appearances of nodular glomeruli in DN and AN. This study highlights how facilitates slide-level annotations to dataset-level observations and hypothesis testing. Scale bars indicate 50m.,yes
PMC5972287,Figure_1,oa_package/d3/01/PMC5972287.tar.gz,['Increased density of macrophages in the red pulp (RP) after experimental stroke.'],"Figure 1 Increased density of macrophages in the red pulp (RP) after experimental stroke. Immunofluorescent labeling of monocyte-derived RPM (blue, CD11b) or both tissue resident and monocyte-derived RPM (red, F4/80) in the splenic RP from nave mice, mice recovered 1 to 7days after middle cerebral artery occlusion (MCAO) or sham-operated mice. Quantification of immunolabelling shows increased percentage area of CD11b and F4/80 immunolabelling per section in mice 15 days or 17 days, respectively, after experimental stroke in comparison to sham-operated or nave controls ( = = = = = = ). Flow cytometry analysis of the spleen shows that the absolute number of CD11b and F4/80 cells is reduced 2 days after stroke in comparison to sham-operated animals ( = = ). Immunofluorescent labeling of CD11b (blue) alongside markers for macrophages (red, F4/80), monocytes (red, Ly6C), and neutrophils (red Ly6G; SJC4) in spleens from mice recovered 2days after MCAO ( = ). Immunofluorescent labeling of CD11b (blue) and SJC4 (red) in the splenic RP from nave mice, mice recovered 1 to 7days after MCAO or sham-operated mice. Quantification of CD11b and SJC4 shows the percentage of CD11b immunolabelling that co-localizes with SJC4 is not significantly altered 17 days after MCAO ( = = = = = = ). Analysis of spleen microarray data shows significantly increased transcription of at 15 days and at 2 days after MCAO in comparison to sham-operated controls ( = = = ). RT-qPCR analysis confirmed a trend for upregulation of mRNA after experimental stroke. RT-qPCR analysis of mRNA confirmed upregulation at 25days after MCAO in comparison to sham operated animals ( = = = = = = ). Scale bars 200m, 100m. Data show meanSD * <0.05; ** <0.01; *** <0.05 one-way analysis of variance with Bonferonni correction; unpaired -test.",yes
PMC7283950,Figure_7,oa_package/6e/48/PMC7283950.tar.gz,"['DiscussionSSP is most likely to develop in patients with COPD, malignancy, pulmonary fibrosis, bronchial asthma or tuberculosis [2].']",Fig. 7 Chest x-ray was taken two and a half years following the procedure showing complete lung expansion with no evidence of pneumothorax.,yes
PMC10693379,Figure_1,oa_package/71/3e/PMC10693379.tar.gz,[' Chest X-ray showing implant in the left lung (arrow).'],Figure 1 Chest X-ray showing implant in the left lung (arrow).,yes
PMC7509698,Figure_4,oa_package/a4/31/PMC7509698.tar.gz,"['One study found that asymptomatic baseball pitchers commonly had ligamentous thickening, bone osteophytes, and tendinopathy in the posteromedial compartment of the elbow [52] ().', 'Adaptive changes in throwing elbow.']",Figure 4 Adaptive changes in throwing elbow. 36-year-old asymptomatic professional baseball pitcher with adaptive ulnar collateral ligament (UCL) thickening. Coronal oblique proton density-weighted fat-suppressed magnetic resonance imaging shows thickening of the anterior bundle of the UCL (arrow),yes
PMC6098965,Figure_6,oa_package/35/f7/PMC6098965.tar.gz,"['05 compared with A and A -vehicle groupHistological verification of cannula of amyloid-beta (A ) injection sites in the CA1 regions of the hippocampus (Left side).', '\nHistology\n\n at right side presents the photomicrograph of needle trace for hippocampus CA1 area microinjection of A or vehicle.', 'Left side of shows the coronal section schematic illustration, which was taken from the Paxinos and Watson rat brain atlas ().']",Figure 6 Histological verification of cannula of amyloid-beta (A) injection sites in the CA1 regions of the hippocampus (Left side). Right side of the Figure shows the approximate location of the injection needle tip in the CA1 region (indicated by the black arrows),yes
PMC9358607,Figure_6,oa_package/2a/af/PMC9358607.tar.gz,"['The number of SF was defined in the axial section (A-D).', '\nThe numbers of Stenson foramen in axial section.']",Figure 6 The numbers of Stenson foramen in axial section. A = one; B = two; C = three; D = four.,yes
PMC9206249,Figure_3,oa_package/21/d8/PMC9206249.tar.gz,"[' 3a).', '3c d).', '3b.', 'a x-ray pelvis showing mild bilateral acetabular dysplasia with a shallow acetabulum, (b) x-ray pelvis showing short and broad femoral neck with coxa vara and a minimal sclerosis of both acetabular roofs (c) US right hip shows a moderate effusion (d) US right knee shows a moderately large effusion in the suprapatellar pouchGiven the combination of camptodactyly, non- inflammatory arthropathy and previous pericarditis a diagnosis of Camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP) was considered and targeted exome sequencing revealed a homozygous PRG4 pathogenic variant (c.']","Fig. 3 x-ray pelvis showing mild bilateral acetabular dysplasia with a shallow acetabulum, ( ) x-ray pelvis showing short and broad femoral neck with coxa vara and a minimal sclerosis of both acetabular roofs ( ) US right hip shows a moderate effusion (d) US right knee shows a moderately large effusion in the suprapatellar pouch",yes
PMC2683749,Figure_3,oa_package/2b/d1/PMC2683749.tar.gz,"['Mitochondrial ATP-Production Rate and Respiratory Chain Enzyme ActivityWe found a decreased rate of ATP production in isolated muscle mitochondria from affected animals (A; One of the five affected animals that contributed to the data presented in did neither show decreases in ATP production nor in respiratory chain enzyme activities, and was therefore not possible to distinguish from the healthy controls with these assays.']",10.1371/journal.pgen.1000499.g003,yes
PMC7433065,Figure_2,oa_package/02/fd/PMC7433065.tar.gz,"['2).', 'a Right breast lump.', 'b Radiological image of the breast lesionIn the histological study of the microbiopsy of the breast lesion, a malignant neoplasm compatible with high-grade sarcoma with areas of necrosis was observed.']",Fig. 2 Right breast lump. Radiological image of the breast lesion,yes
PMC8064403,Figure_6,oa_package/2f/5d/PMC8064403.tar.gz,"['Effect of cell line was determined using an unpaired two-tailed t-test (I L).', 'Immunoblotting of cell lysates revealed that while SH-SY5Y and N2A cells express similar levels of FUS protein, SH-SY5Y cells express much higher levels of total DNA-PK compared to N2A cells (A).', 'Treatment of either cell line with CLM did not change the total levels of DNA-PK (A).', 'In contrast, we did not detect phosphorylation of DNA-PK or FUS in N2A cells via immunoblot at any dose of CLM tested (B).', 'The overall fluorescent intensity for DNA-PK was significantly higher in SH-SY5Y compared to N2A cells, confirming our western blot results (C, E).', 'Intriguingly, the DNA-PK signal appeared more diffuse throughout the cytoplasm and the nucleus of N2A cells, while DNA-PK immunoreactivity in SH-SY5Y cells was more predominant in the nucleus (C).', 'Quantification of immunofluorescence confirmed the presence of significantly more DNA-PK in the nucleus of SH-SY5Y compared to N2A regardless of treatment (F).', 'Furthermore, CLM treatment for either SH-SY5Y or N2A cells did not change the cellular localization of DNA-PK (F).', 'In line with this, the proportion of DNA-PK signal remained unchanged (around ~1) between control and treatment for both SH-SY5Y and N2A cells when examining the whole cell (I) and nucleus (', 'In untreated control cells, p-DNA-PK staining in both N2A and SH-SY5Y cells was weak and diffuse throughout the nucleus and cytoplasm (D).', 'As expected, SH-SY5Y cells had robust DNA-PK activation following CLM treatment, as measured by phosphorylation of the S2056 (S2053 for N2A cells) residue on DNA-PK (D, G).', 'Unexpectedly, N2A cells exhibited an increase in p-DNA-PK whole cell signal (G) and nuclear signal (', '0442; K) and the nucleus (p = 0.', '0389; L).', '.']","Fig. 6. Compared to human cells, mouse cells have decreased levels of DNA-PK and activation following double strand DNA breaks induced by CLM treatment. SH-SY5Y cells show a distinct increase in activated DNA-PK whereas N2A cells lack a significant DNA-PK response following CLM treatment by western blot. SH-SY5Y and N2A cells were treated with increasing concentrations of CLM for 2 h. Following treatment, RIPA extracted whole cell lysates were analyzed for (A) total and (B) activated DNA-PK signal using the following antibodies: DNA-PK, p-DNA-PK, FUS, p-FUS (Ser30), and GAPDH. (C/D) N2A cells have lower total and activated DNA-PK following CLM as compared to SH-SY5Y cells by immunofluorescence. SH-SY5Y and N2A cells were treated with DMSO (control) or CLM (20 nM CLM) for 2 h and stained for (C) total and (D) activated DNA-PK. Nuclei were counter-stained with DAPI. Four images with an average of 527 cells each were used for quantification per replicate (n = 3). Total and activated DNA-PK signal was quantified for both the (E/G) whole cell and (F/H) nucleus. SH-SY5Y cells show robust (E/F) total and (G/H) activated DNA-PK (p-DNA-PK, S2056) signal following CLM while N2A cell signal remains modest in presence of CLM. (I/J/K/L)The ratio of the signal from treated (20 nM CLM) to the signal from untreated (control) cells was calculated for each graph. Error bars on graphs indicate mean SEM.",yes
PMC8304165,Figure_7,oa_package/54/b8/PMC8304165.tar.gz,"['The subsequent immunostaining performed in hSC lines also showed no detection of CaSR protein in satellite cells (A,B).', 'Human PTcs were used as a positive control, to validate the method and anti-CaSR antibody (C,D).', 'Immunostaining of CaSR in hSC lines.']","Figure 7 Immunostaining of CaSR in hSC lines. The analysis of CaSR protein in hSC lines: ( ) negative control with only secondary antibody and ( ) with primary anti-CaSR antibody. Results are representative of experiments carried out in three established hSC lines. The analysis of CaSR protein was also performed in human parathyroid cells, used as positive control, ( ) negative control with only secondary antibody, and ( ) with primary anti-CaSR antibody. LSCM conventional colors: green for CaSR protein and red for nuclei, original magnification: 20, scale bar: 50 m.",yes
PMC4553688,Figure_3,oa_package/04/a9/PMC4553688.tar.gz,"['Histologic findings reveal numerous foci of lymphocytic inflammatory cells and necrotic myocytes in the atrial and ventricular walls which were compatible with fulminant lymphocytic myocarditis of Dallas criteria (5) ().', 'Histologic findings of the explant heart.']","Fig. 3 Histologic findings of the explant heart. ( ) Left ventricle, ( ) Right ventricle, ( ) Left atrium, and ( ) Right atrium. Both atrial and ventricular walls show fulminant lymphocytic myocarditis. There are no giant cells or evidence for vasculitis or granulomas (H&E stain 200).",yes
PMC9754254,Figure_3,oa_package/2d/18/PMC9754254.tar.gz,"['Patch classifier model was trained to accurately infer three correct labels (classes M, D, and N) for each patch image ().', 'g003Concept of patch classifier training.']",10.1371/journal.pone.0278542.g003,yes
PMC5467636,Figure_1,oa_package/de/d9/PMC5467636.tar.gz,"['In post-traumatic cases, if radiographs do not show abnormalities, a bone scan can be performed and positive scans can be followed by CT ().', '160055"" ref-type=""bibr"">6MRI illustrating os trigonum and intra-operative view after release of symptomatic os trigonum.']",Fig. 1 MRI illustrating os trigonum and intra-operative view after release of symptomatic os trigonum.,yes
PMC11423784,Figure_2,oa_package/3b/8d/PMC11423784.tar.gz,[],"Figure 2 Preoperative T1-weighted MR image with contrast in sagittal view (arrow showing the intraspinal mass, compressing the spinal cord at the T4 level) MR:magnetic resonance",yes
PMC11345806,Figure_3,oa_package/b8/31/PMC11345806.tar.gz,"['Sub-pleural dot-like hemorrhagesThe heart was enlarged, with mild hypertrophy of the left ventricle.']",Figure 3 Sub-pleural dot-like hemorrhages,yes
PMC10741119,Figure_8,oa_package/77/90/PMC10741119.tar.gz,"['a c show the images of a malignant lesion using different imaging methods on the same patient.', '(a c) ABUS and CEM images show verified right breast cancer.']","Figure 8 ( ) ABUS and CEM images show verified right breast cancer. ( ) Coronal and transverse planes, visible hypoechoic mass between the lines. ( ) Subtraction CEM image in RCC and RMLO projections, visible oval enhancing mass in the upper outer quadrant. ( ) FFDM image in RCC and RMLO projections, mass not visible.",yes
PMC3661554,Figure_2,oa_package/94/90/PMC3661554.tar.gz,"['The protein profiles obtained by native PAGE (, Upper panel) showed a gradual fading of the red NAF-1 and concomitant appearance of red holo-Fd over time, indicating [2Fe-2S] cluster transfer from NAF-1 to apo-Fd with no changes in the respective protein levels, as shown by Coomassie Blue staining.', 'The progress of the transfer to apo-Fd was assessed spectrophotometrically and is depicted in \nLower panel as % of cluster transfer CT (%) with time of incubation (illustrative absorption spectra of apo-Fd, NAF-1 and their interaction are given in [9] as an [2Fe-2S] cluster transfer protein ().']",10.1371/journal.pone.0061202.g002,yes
PMC4307678,Figure_8,oa_package/22/0d/PMC4307678.tar.gz,"['In May 2008, her vision worsened again in her right eye, and she complained of blurred vision without diplopia.']","Figure 8 Abbreviations: o.n., optic nerve; s, suction.",yes
PMC7650138,Figure_3,oa_package/7b/d4/PMC7650138.tar.gz,"[' 3.', '4)Phosphorylated neurofilament heavy chain (pNfH) (pg/ml) (median, 25 75 percentile)663 (480 1022)Zona occludens 1 (ZO-1) (pg/ml) (median, 25 75 percentile)1005 (783 1377)White matter hyperintensities (WMH) on FLAIR imaging and corresponding optical coherence tomography (OCT) scans and intensity graphs.', 'e and f intensity graphs showing the inner wall s (red line) and the outer wall s peak (blue line) with space in between (orange line) corresponding to the lumenRetinal vessel parameters in CSVDVessel parameters assessed in our CSVD patients are shown in Table 2.']","Fig. 3 White matter hyperintensities (WMH) on FLAIR imaging and corresponding optical coherence tomography (OCT) scans and intensity graphs. and Examples of two patients with different extent of WMH on FLAIR imaging (yellow arrows), left Fazekas grade 3, right Fazekas grade 1, respectively. and OCT scans showing superior temporal branch of the retinal artery (red arrow: inner wall. Blue arrow: outer wall. Orange line: lumen). and intensity graphs showing the inner walls (red line) and the outer walls peak (blue line) with space in between (orange line) corresponding to the lumen",yes
PMC4624747,Figure_1,oa_package/fa/e2/PMC4624747.tar.gz,['A An elderly woman taking ramipril with pemphigus vulgaris (PV) at active Th2 mediated stage.'],Figure 1 An elderly woman taking ramipril with pemphigus vulgaris (PV) at active Th2 mediated stage. Suprabasal acantholysis of PV. Row of tombstones appearance of basal layer (H + E stain). Indirect immunofluorescence revealing serum IgG4 pemphigus antibodies. An elderly woman taking indapamide with pemphigus foliaceus (PF) relapse at active Th2 mediated stage. Subcorneal acantholytic separation of PF (H + E stain). Direct immunofluorescence of the perilesional skin revealing IgG4 pemphigus deposits in interfollicular and intrafollicular epithelium,yes
PMC5568620,Figure_4,oa_package/3d/ef/PMC5568620.tar.gz,"['5 cm in diameter, using mechanical instruments and/or bipolar electrodes ().', '003""/>Evacuation of hypothesized superficial adenomyotic cysts with a 5-Fr bipolar electrode (KARL STORZ, Germany).']","Figure 4 Evacuation of hypothesized superficial adenomyotic cysts with a 5-Fr bipolar electrode (KARL STORZ, Germany). Panoramic image of the small cystic lesion (a). Incision and drainage of the cystic lesion (b-c).",yes
PMC10506826,Figure_2,oa_package/5a/f9/PMC10506826.tar.gz,[],"Figure 2 Lymphangitis over upper limbs after (a) intradermal tuberculin antigen, (b) spider bite, and (c) mosquito bite",yes
PMC9738282,Figure_2,oa_package/90/01/PMC9738282.tar.gz,"['Insulin lowers the blood glucose level and glucagon raises it; glucagon receptors are found in liver cells, which break down stored glycogen into glucose and release glucose in the blood, the glucose-dependent stage in human insulin regulation that does not work correctly in T2DM () [4,5].', 'Regulation of blood glucose occurs through insulin.']",Figure 2 Regulation of blood glucose occurs through insulin.,yes
PMC5009287,Figure_7,oa_package/45/c7/PMC5009287.tar.gz,"['These interactions were monitored in our MD simulations () except the interaction involving the hydroxyl group of Hyp (interaction 7 in the list), which is not included in the complexes because Hyp is absent in our recombinant bacterial collagen.', 'It appears that the local perturbations in the triple helix near Ser-502 affect the relation between the collagen A and B chains and the integrin side chains ().']","FIGURE 7. , the key interactions that participate in the integrin-collagen binding and the residues involved in each interaction. The residues from integrin and chains A and B of collagen are colored in , , and , respectively. All these interactions are observed to be stable in the 100-ns MD simulation of integrin with WT collagen (indicated by + ). The van der Waals interaction of collagen Phe-503 with integrin Gln-215 and Asn-154 is disrupted by the Gly to Ser mutations at position 502 (indicated by the ). and , the simulation structures of integrin with WT collagen ( ) and integrin with G502S mutant ( ). Chains A, B, and C for collagens are colored in , , and , respectively. The Mg ions are represented by . The substituted Ser residues are shown in . The disrupted van der Waals interaction is illustrated.",yes
PMC11337201,Figure_3,oa_package/23/de/PMC11337201.tar.gz,"['We have showcased some images from these publicly available datasets in .', 'Partial examples of images from each exposed data set.', 'It can be clearly found in the that the most obvious problem of the psoriasis image is the lack of standardization of the data.']","Figure 3 Partial examples of images from each exposed data set. Pso, Psoriasis. Reprinted with permission of six watermarked images from the DermNetNZ dataset, which is labeled as Guttate Pso, Chronic plaque Pso, Flexural Pso, Scalp Pso, Sebopsoriasis, and Nail Pso, are from , DermNet , licensed under CC BY-NC-ND 3.0 NZ. For the DermNetNZ dataset, the links to the individual images are as follows: Guttate Pso, ; Chronic plaque Pso, ; Flexural Pso, ; Scalp Pso, ; Sebopsoriasis, ; Nail Pso, . Reprinted with permission of three watermarked images from the Dermatology Atlas dataset, which is labeled as Artropathic Pso, Pso After Erysipelas, and Pustular Pso, are from . For the Dermatology Atlas dataset, the links to the individual images are as follows: Artropathic Pso, ; Pso After Erysipelas, ; Pustular Pso, . Reprinted with permission of three images from the Hellenic Dermatology Atlas dataset, which is labeled as Generalized Pso, Guttate Pso, and Palque Pso, are from . For the Hellenic Dermatology Atlas dataset, the links to the individual images are as follows: Generalized Pso, ; Guttate Pso, ; Palque Pso, .",yes
PMC3905351,Figure_4,oa_package/90/5c/PMC3905351.tar.gz,[],"Fig. (4) Follow-up axial nonenhanced soft tissue window CT ( ) of the head showing complete resolution of the soft tissue thickening andfluid collection in the left temporal bone region. Axial bone window CT ( ) shows interval remineralization of the mastoid bone(arrowheads), including the area surrounding the semicircular canals and facial nerve canal (arrow). On the right, the fluid in the mastoid aircells has resolved and interval mineralization is noted.",yes
PMC8554238,Figure_1,oa_package/d1/68/PMC8554238.tar.gz,"['cruzi Infection in C2C12 Myoblasts and Macrophages Through NO-Dependent and -Independent Mechanisms, RespectivelyNone of the stimuli used altered the cell viability, of myoblasts and macrophages at the tested concentrations (Supplementary ).', 'cruzi TCTs (pretreatment) significantly reduced the number of infected cells and intracellular parasites in the C2C12 myoblasts and bone marrow-derived macrophages ().', 'This reduced parasite infection was abrogated when the cells were preincubated with 9CI, a neutralizing antibody against IL-9 ().', 'rIL9 decreased the number of infected cells and internalized parasites in C2C12 cells and macrophages, and IL-9 neutralization (9CI) reversed the effect.']","Figure 1 rIL9 decreased the number of infected cells and internalized parasites in C2C12 cells and macrophages, and IL-9 neutralization (9CI) reversed the effect. C2C12 cells and macrophage were pretreated for 24 h with rIL9 (25 ng/mL or 10 ng/mL) or 9CI (1.25 g/mL) and then infected with strain Y for 3 h. The cells were fixed with Bouins solution and then Giemsa stained. Graphs show the percentage of infected cells and number of internalized parasites . Students t-tests **p = 0.0172, *p < 0.001. Control: cells infected and cultured just in culture medium.",yes
PMC10590653,Figure_2,oa_package/de/7a/PMC10590653.tar.gz,"['A high-grade SBO was noted, possibly secondary to stricture, with transition point in the right lower quadrant ().', 'CT Angiography showing SBO with transition point in the right lower quadrant.']",Figure 2 CT Angiography showing SBO with transition point in the right lower quadrant. A loop of thickened ileum measures 30.4mm. The white arrow points to a stricture.,yes
PMC4392949,Figure_4,oa_package/1a/1f/PMC4392949.tar.gz,"['One specimen with diaphragmatic impressions, that is, Netter type 7 [4] liver, was also noted in the present study ().', '003""/>Shows diaphragmatic impressions on superior surface of right lobe of liver.']",Figure 4 Shows diaphragmatic impressions on superior surface of right lobe of liver.,yes
PMC9431809,Figure_8,oa_package/31/4a/PMC9431809.tar.gz,"[' (cor triatriatum sinister) (fibromuscular membrane) ().', 'LA = left atrium, LV = left ventricleCor triatriatum sinister in a 21-year-old woman.']","Fig. 8 Cor triatriatum sinister in a 21-year-old woman. CT angiography reveals markedly enlarged LA with a thin fibromuscular membrane (arrows) bisecting the cavity. The proximal chamber is contiguous to the pulmonary venous confluence, and bulging distal chamber is connected to an enlarged LAA. The RA is also enlarged. A small fenestration (13 mm, arrowhead) is detected at the lower portion of the membrane, allowing decompression of the pulmonary veins. LA = left atrium, LAA = left atrial appendage, LV = left ventricle, RA = right atrium",yes
PMC2766270,Figure_2,oa_package/9a/1c/PMC2766270.tar.gz,"['Distribution of DCX+ cells in the temporal lobe cortical areas surrounding the amygdala (A K), in the insular (Ins) and secondary somatosensory (S2) cortex (L), and in the ventral prefrontal cortex including the medial and lateral orbital gyri (MOG, LOG) (M O) in a 22-year-old rhesus monkey.']","Figure 2 . Framed areas in lower magnification panels are enlarged sequentially as indicated. DCX+ cells in the amygdala (Amyg) are located near the white matter deep to the entorhinal cortex (Ent) as a cellular band, which continues into the adjoining Ent and further dorsally into the inferior temporal gyrus (ITG) around the border of layers I and II . Labeled cells are mostly small and bipolar, often arrange as clusters and chains with cells seemingly migrate outside-in intracortically . However, in the ITG a large number of cells are associated with tangentially arranged migratory chains that can extend very long . Some of these chains appear to extend from layer I and then enter the cortical plate, with cells dispatching or being dispersed around the end of the chains ( , green arrows). Note that labeled cells are also fairly common in the MOG and LOG , but fewer in the Ins cortex and S2 . A few relatively large cells with reduced DCX reactivity are present in layers II/III (indicated with black arrows in ). rf, rhinal fissure; LF, lateral fissure. Arab numbers indicate cortical layers. Scale bar=3mm in applying to , equivalent to 1mm for , 500 m for , 100 m for and 50 m for .",yes
PMC11512103,Figure_3,oa_package/c3/44/PMC11512103.tar.gz,['.'],"Fig. 3. (A) Representative immunofluorescence images showing the NMJ innervation pattern categorised as full partial and denervated junctions. Sections were stained with BTX (red) and a combination of anti-neurofilament (NF; green) and anti-synapsin-1 (Syn; green) as detailed in . (B) Quantification of the relative percentage of endplates exhibiting full, partial and denervated junctions in 10-month-old LKO animals compared to age-matched WT control animals. Each bar represents the means.e.m.; 70-100 NMJs were analysed per animal, and three animals were used for each genotype (WT and LKO) (two-way ANOVA with Sidak's multiple comparison test; **** <0.0001). (C-E) Immunofluorescence images representing the phenomenon of collateral sprouting at NMJs. (C) In normal innervated NMJ, the presynaptic axon terminal innervates only one motor endplate without collateral sprouting. (D) In the case of preterminal sprouting, the motor endplate is innervated by a newly formed thin axonal branch originating from an area immediately proximal to the NMJ (white arrow). (E) In the case of ultra-terminal sprouting, new axonal sprouts emanate from but grow beyond the endplate region to innervate the nearby denervated junction (white arrow). (F) Relative percentage of endplates with collateral sprouting in 10-month-old LKO animals compared to age-matched WT control animals. Note the increased frequency of collateral sprouting in the 10-month age group compared with WT control animals. Each bar represents the means.e.m.; 70-100 NMJs were analysed per animal, and three animals were used for each genotype (WT and LKO) (unpaired two-tailed Student's test; * <0.05). Scale bars: 10m.",yes
PMC9420090,Figure_4,oa_package/f5/fb/PMC9420090.tar.gz,[' 4).'],Fig. 4 Results of the molecular and immunohistochemical study of alterations of cancer-related genes in the four morphologically distinct lesions find in the ovary and in the endometrium (see text for details),yes
PMC3691094,Figure_4,oa_package/02/59/PMC3691094.tar.gz,"['The treatment included a resection with wide margins and a reconstruction of the femur with a contralateral vascularized fibula, a homologous allograft and a plate ().']",FIGURE 3A The tumour is composed of a well differentiated fibroblastic component entrapping bony . The spindle cells are set in a collagenous matrix (H&E stain).,yes
PMC7719782,Figure_1,oa_package/c3/34/PMC7719782.tar.gz,"['Representative immunostaining of NFs in the myenteric plexus is shown in .', '017) (E).', '281) (F).', 'Immunostaining of neurofilaments (NFs) in the myenteric plexus.']","Figure 1 Immunostaining of neurofilaments (NFs) in the myenteric plexus. Immunostaining of NFs in the myenteric plexus. Representative image of the myenteric nerve plexus located between the two muscle layers (longitudinal and circular muscles), comprised of many nerve plexus sections on the cut surface . Fine staining of NF-positive profiles (brown color) could be seen in the perikaryon of neurons in the myenteric plexus. The nuclei of the neurons were lightly stained and some nucleoli could be seen (black arrows in ). Hematoxylin counterstain, scale bars = 100 m , 50 m . Quantitative analysis of the NF-positive neuron count in the myenteric plexus. The count is subdivided into the ileum (Il), right colon (RC), left colon (LC), and rectum (RE). Inter-group differences are reported as per one-way ANOVA ( = 3.564, = 18, = 0.040) followed by an LSD-t test (RC group 1.07 0.82 . the LC group 3.66 0.62, = 0.010; RC group 1.07 0.82 . the RE group 3.11 1.35, = 0. 017). Data are shown as NF positive neurons per visual field for individual cases and the group mean SD is also shown. Scatter plot of the NF-positive neuron count within the myenteric plexus with age. No significant correlation was observed (Pearson correlation, = 0.499). * = < 0.05.",yes
PMC10721145,Figure_3,oa_package/e6/5a/PMC10721145.tar.gz,"['Both groups had preserved ejection fraction (, A and B, and Supplemental Table 2) and experienced increases in systolic blood pressure compared with STD-fed controls (Supplemental A).', 'Whereas 5 weeks of H/L induced, as expected, impaired relaxation in WT mice as measured by the slope of the end-diastolic pressure volume relationship (EDPVR), Tcra / mice did not show such an increase, demonstrating preserved cardiac relaxation compared with WT (C).', 'Moreover, the cardiometabolic stress induced by H/L resulted in LV and cardiomyocyte hypertrophy in WT mice exclusively, and not in Tcra / mice, as shown by gross organ weight and wheat germ agglutinin staining of cardiac sections, respectively (, D F).', 'To uncover the mechanisms by which T cells contribute to diastolic dysfunction in response to H/L, we next focused on investigating the protein expression of key mediators of cardiomyocyte relaxation: sarco(endo)plasmic reticulum Ca++-ATPase (SERCA), responsible for cytoplasmic calcium import into the sarcoplasmic reticulum to perpetuate relaxation; and phospholamban (PLN), an inhibitor of SERCA when in an unphosphorylated state (20) (G).', 'Strikingly, the decrease in the phosphorylated to total PLN was not observed in Tcra / mice fed H/L compared with STD controls, whereas SERCA was also unaltered in Tcra / mice fed H/L or STD (, H and I).', 'OTII mice also had increased renal CD4+ T cell abundance in response to H/L compared with STD controls (Supplemental , D and E).', 'As expected, the expression of myocardial Xbp1s was reduced in mice subjected to 5 weeks of H/L, compared with STD and TAC mice, which presented with reduced ejection fraction, LV hypertrophy, and thickening of both the anterior and posterior walls (Supplemental , F I, and Supplemental Table 4), reinforcing that downregulation of myocardial UPR is HFpEF specific.', 'In contrast, no changes in the expression of any of these UPR genes were observed in total peripheral blood mononuclear cells (PBMCs) between H/L-fed mice and STD-fed controls (Supplemental K).', 'Interestingly, after 5 weeks of H/L, splenic CD8+ T cells from H/L-fed mice also manifested significantly decreased expression of Xbp1s, but a complete preservation of Atf4 (Supplemental L).', 'Furthermore, splenic CD4+ T cells from female WT mice given H/L did not have significant downregulation of spliced or total Xbp1 (Supplemental M).', 'Diastolic dysfunction and cardiac hypertrophy are not induced by H/L in T cell deficient mice.']","Figure 3 Diastolic dysfunction and cardiac hypertrophy are not induced by H/L in T celldeficient mice. ( ) The ejection fraction ( and ) and end-diastolic pressurevolume relationship (EDPVR; ) were measured in WT or mice fed H/L or STD for 5 weeks using echocardiography and invasive hemodynamic analysis, respectively. ( ) The LV weight of WT or mice was measured and normalized to tibia length (LV/TL). ( and ) LV cryosections from these mice were stained with wheat germ agglutinin and analyzed for cardiomyocyte area using ImageJ. Scale bars: 50 m. ( ) The expression of SERCA, phospho-PLN, and total PLN was measured in LV samples using Western blotting. = 512. Error bars represent the mean SEM. Two-way ANOVA with idks multiple-comparison test. * 0.05, ** 0.01, *** 0.001. This figure was created using Biorender.com.",yes
PMC5642507,Figure_9,oa_package/89/c2/PMC5642507.tar.gz,"['Olfactory bulb protein expression of EGFR, CREB1, TGF-beta, c-Jun and STAT3 across proteinopathiesOB Protein expression was documented by Western blot.']","Figure 9 Olfactory bulb protein expression of EGFR, CREB1, TGF-beta, c-Jun and STAT3 across proteinopathies OB Protein expression was documented by Western blot. ( ) EGFR expression, ( ) TGF-beta expression, ( ) c-Jun expression, ( ) STAT3/phospho-STAT3 (Y705) expression, and ( ) CREB/phospho-CREB (S133) expression, in PSP, FTLD, and mixed dementia subjects. Graphs represent histograms of band densities. Data are presented as mean SEM from: Controls ( = 4 cases), PSP ( = 9 cases), FTLD ( = 6 cases), and mixed dementia (mix AD VD) ( = 9 cases). * < 0.05 vs control group; ** < 0.01 vs control group.",yes
PMC9320967,Figure_1,oa_package/f0/61/PMC9320967.tar.gz,['785 ej \n\n Secukinumab TC in function of time on secukinumab treatment.'],"Figure 1 Secukinumab TC in function of time on secukinumab treatment. The dots of the same colour represent the concentrations of the same individual. The black dashed line represents the mean TC time course of a typical patient (BMI 27.1kg/m ). The mathematical equationof the mean TC time course of a typical patient is shown. BMI, body mass index; TC, trough concentration. [Colour figure can be viewed at ]",yes
PMC5426035,Figure_2,oa_package/fe/3a/PMC5426035.tar.gz,"['Microscopic evaluation revealed granulomatous inflammation of the testis and tunica albuginea with abscess formation due to a degenerating filarial nematode (', ' 2Granulomatous inflammation and abscess formation due to degenerating filarial nematode.']",Figure2 Granulomatous inflammation and abscess formation due to degenerating filarial nematode. Seminiferous tubules can be seen in the bottom right.,yes
PMC7601842,Figure_6,oa_package/f6/f4/PMC7601842.tar.gz,"['In 16/21 CBs, we were able to perform immunocytochemistry to subclassify NHL according to the 2017 WHO classification; in four cases, FISH was also performed and allowed to confirm MCL (cases 20, 47; , case 20) and to classify diffuse large B-cell lymphoma as post germinal center type (case 21), respectively.', 'Mantle Cell lymphoma, case 20.']","Figure 6 Mantle Cell lymphoma, case 20. HematoxylinEosin CB-stained section shows small monomorphic lymphocytes (20) ( ). CD5 stains small lymphocytes (20) ( ). CyclinD1 expression in neoplastic cells (20) ( ). FISH analysis showing the t(11;14)(q13; q32) translocation. The red arrow indicates neoplastic cells containing single red and green signals (corresponding to the normal CCND1 and IGH loci, respectively) together with two fused signals (corresponding to the genes fused by the translocation). Normal cells are indicated by the white arrow ( ).",yes
PMC2853529,Figure_1,oa_package/57/c6/PMC2853529.tar.gz,['Identification of serum IL-6 as a marker of elevated ICP by antibody arrays.'],"Figure 1 . . Time course of ICP in patients classified as having elevated ICP (ICP 25 mm Hg) versus that recorded in patients whose ICP remained below 20 mm Hg for the duration of the sampling period. . Representative picture of a Ray Biosciences Cytokine array showing the immunoreactivity of interleukin family members in pooled samples (n = 7/pool) from healthy volunteers, TBI patients with elevated ICP, and TBI patients whose ICP remained below 20 mm Hg for the duration of the 5 day sampling period. . Summary data (presented as % healthy volunteer(HV)) showing that the relative signal of IL-6 is significantly increased in patients with ICP 25 mm Hg compared to patients whose ICP remained below 20 mm Hg throughout the study period. Data is presented as mean SEM. , significant difference by two-way ANOVA.",yes
PMC4414289,Figure_6,oa_package/2a/11/PMC4414289.tar.gz,"['After co-culture with MSCs for two days, the expression of FoxP3 slightly changed ( 6A).', 'Mechanically, phosphorylation-Smad2 was activated after 30 minutes treatment with MSCs while JNK and p38 signaling showed no change ( 6B and C).', 'MSCs increase the expression of FoxP3 in naive CD4+T cells.', 'DiscussionAs it is difficult to cure tumors directly, many reports have focused on anti-inflammation to inhibit the initiation and progression of tumors [20].']","Figure 6 MSCs increase the expression of FoxP3 in naive CD4 T cells. Naive CD4 T cells co-cultured with MSCs (ratio 1:5)/TGF- (5 ng/ml) or without MSCs/TGF- in the presence of anti-CD3/CD28 (2 g/ml each). T cells were harvested from different treatment groups to assess Foxp3 expression by flow cytometry. MSCs conditioned medium was collected after 48 hours and 72 hours, and then used to stimulate Jurkat cells for different times. were harvested to examine TGF- signaling by Western blotting of Smad2 and phospho-Smad2. TCR signaling was examined by Western blotting of JNK, phospho-JNK, p38 and phospho-p38. The protein level of p-Smad2 was analyzed using Quantity One. Values are expressed as meansSEM. * <0.05 or ** <0.01. MSCs, mesenchymal stem cells; SEM, standard error of the mean; TCR, T cell receptor; TGF-, transforming growth factor-.",yes
PMC9206905,Figure_5,oa_package/bc/96/PMC9206905.tar.gz,[''],"Fig. 5 noncaseating granuloma. (A) tuberculoma in the frontal and suprasellar regions, hypointense in the T1-weighted axial image, (B) hyperintense in the T2-weighted axial image, and (C) ring contrast enhancement of the tuberculoma on the post-contrast image.",yes
PMC9468358,Figure_2,oa_package/99/01/PMC9468358.tar.gz,"['.', 'Immunohistochemical characterization of lymphoplasmablastic lymphoma, with positive staining for CD38 (A) and restriction of Kappa light chains (B) vs.', 'The patient is currently undergoing follow-up and treatment by hematology and oncology, who defined no indication of adjuvant therapy at the moment for adenocarcinoma but started treatment for lymphoma with CHOP therapy (Cyclophosphamide, Doxorubicin, Vincristine, and prednisone).']","Fig. 2 Immunohistochemical characterization of lymphoplasmablastic lymphoma, with positive staining for CD38 (A) and restriction of Kappa light chains (B) vs. Lambda (C).",yes
PMC3271449,Figure_4,oa_package/19/6a/PMC3271449.tar.gz,[],Figure 4 Immunohistochemical examination of the tumor was positive with Desmin immunostaining,yes
PMC8018033,Figure_4,oa_package/a8/ec/PMC8018033.tar.gz,['\nPericyte vascular network in cleared brain samples.'],"Figure 4 . and 3Drendering of stitched and processed twophoton images from two cleared specimens, one nondemented control case ( ) and one AD case ( ) showing PDGFRimmunoreactive (red) and Aimmunoreactive (green) signals. The spurious green signal in the surface of represent contaminating particles of the tissue piece. The density of PDGFRimmunoreactive structures is similar between the control and AD tissue. raw scanning images of the signal corresponding to PDGFRimmunoreactivity. Under the imaging protocol employed for the sampling of large tissue volumes, twophoton imaging did not resolve the fine cellular structures of pericytes. However, the staining revealed unambiguously the lining of PDGFRimmunoreactive mural cells in arterioles, capillaries and venules. Scale bars: 400m.",yes
PMC8770606,Figure_1,oa_package/35/8b/PMC8770606.tar.gz,[],"FIG 1 Feature map of synuclein, cAbl, and parkin. ( ) Synuclein is a small, irregularly structured protein of 140 amino acids, which is comprised of a 61 amino acid amphipathic helical bundle where both the cAbl phosphorylation site at Tyr (Y39) and one of six disease associated point mutants (A53T) is located and associated with inherited Parkinson's disease. The nonamyloidcomponent (NAC) is followed by an irregularly structured acidic region where both Tyr and Ser reside, whereas Tyr resides in the amphipathic coiledcoil region. Above these chemical modification sites are the putative kinases implicated in phosphorylation of these sites. ( ) cAbl is a large, multidomain protein of 1142 amino acids, wherein the regulatory regions of the myristylation cap, SH3, and SH2 domains reside in the Nterminal half of the protein, followed by tyrosine kinase domain. The kinase core of the cAbl protein has a domain organization similar to that of the Src family kinases, with sequential Src homology (SH) 3 and SH2 domains, an SH2/kinase linker, and a bilobed kinase domain. This core is flanked by an Nterminal cap (Ncap) region with a signal sequence for myristylation, which serves dual roles in regulation of kinase activity and in membrane localization. Cterminal to the kinase domain is a long region of >600 amino acids encoded by a single exon, which controls interaction of Abl with other SH3containing proteins and the actin cytoskeleton. This region also regulates nuclearcytoplasmic shuttling of the kinase. ( ) Parkin is a protein of 465 amino acids consisting of a Ubiquitinlike domain (Ubl) at its N terminus and four zinccoordinating RINGlike domains: RING0, RING1, inbetween RING (IBR) and RING2, and a repressor element of parkin (REP) domain. It is classified as a RINGbetweenRING or RBR E3 ubiquitin ligase. More than 120 pathogenic PD mutations are spread throughout parkin domains, attesting to critical functions for each of the individual domain. The Ubl domain is involved in substrate recognition, binding SH3 and ubiquitin interacting motif (UIM) domains, proteasome association, and regulation of cellular parkin levels and activity.",yes
PMC3551781,Figure_7,oa_package/2e/b4/PMC3551781.tar.gz,['Follicular renewal in adult human ovaries.'],"Figure 7 ) Cytokeratin (CK) positive (brown color) cells of fibroblast type (fb) in tunica albuginea (ta) exhibit mesenchymal-epithelial transition into OSC (osc). Inset shows a transitory stage (fb/e). ) The CK+ epithelial nest (n) inside of the venule (v) in deep ovarian cortex, which extends an arm (a) to catch the oocyte (o, dashed line) from the blood circulation. e = endothelial cell. ) The nest body (n) and closing ""gate"". A portion of the oocyte (dashed line) still lies outside of the complex, and is expected to move inside (arched arrow). The oocyte contains intraooplasmic CK+ extensions from the nest wall (arrowheads), which contribute to the formation of CK+ paranuclear (Balbiani) body (asterisk). The oocyte nucleus is indicated by a dotted line. ) The occupied ""bird's"" nest type indicates a half way oocyte-nest assembly. CK indicates cytokeratin staining of primitive granulosa cells and ZP indicates zona pellucida expression in the assembling oocyte. ) Segments of OSC show cytoplasmic PS1 (meiotically expressed carbohydrate) expression. Asymmetric division of OSC gives rise to cells exhibiting nuclear PS1 (+ nuclei vs. - cell daughters) and descending from the OSC into tunica albuginea (ta). ) In tunica albuginea, the putative germ cells increase in size, show a symmetric division (black arrow) and exhibit development of cytoplasmic PS1 immunoexpression when entering (white arrow) the upper ovarian cortex (uc). ) Association of primary follicles (arrowhead) with the cortical epithelial crypt (ec). Dashed boxes indicate unassembled epithelial nests. Inset shows origination of germ-like cells among CK+ cells (CK) in epithelial crypt. Note ZP+ segment (white arrowhead) associated with unstained round cell nucleus (asterisk). ) Migrating germ cells with tadpole shape (dashed line), unstained nucleus (dotted line) and ZP+ staining of the intermediate segment (arrowhead). ) Some medullary vessels (v) show accumulation of ZP+ (dark color) degenerating oocytes with unstained nuclei (arrowheads). Arrow indicates ZP release. Adapted from[ ], Antonin Bukovsky.",yes
PMC7663268,Figure_1,oa_package/cd/4e/PMC7663268.tar.gz,"['All non-lesional control cases displayed preservation of myelin throughout the periventricular region and an intact ependymal lining (A,B,D,E).', 'In contrast, all PVL cases were characterised by a reduction in luxol fast blue (LFB) staining, indicative of myelin attenuation (C), and partial denudation of the ependyma in four out of seven of the cases (F).', 'Seven cases of radiologically and histologically-rated non-lesional control white matter contained low levels of MHC-II immunoreactive microglia throughout the periventricular region (G); however, four cases contained high levels of MHC-II expression (H).', 'Seven cases of radiologically and histologically-identified PVL contained high levels of MHC-II+ microglia (I).', '01326687838Histological characterisation of periventricular white matter.']","Figure 1 Histological characterisation of periventricular white matter. Luxol fast blue (LFB) ( , ) and H&E staining ( , ) of radiologically normal appearing white matter demonstrated a regular pattern of myelin staining across the periventricular region. In contrast, LFB ( ) and H&E staining ( ) of radiologically identified lesional cases displayed a band of periventricular demyelination. MHC-II immunostaining of radiologically normal appearing white matter identified cases with minimal MHC-II microglia (( ), control) but also cases with high levels of MHC-II immunoreactivity (( ), pre-lesional). Radiologically identified periventricular lesions (PVL) contained high levels of MHC-II microglia ( ). Scale bar represents 500 m in ( ) and 100 m in ( ).",yes
PMC6852495,Figure_2,oa_package/34/46/PMC6852495.tar.gz,['BMPR2 is degraded via lysosomes in an autophagy related fashion.'],"Figure 2 BMPR2 is degraded via lysosomes in an autophagyrelated fashion. (A, B) Western blot analysis of BMPR2 protein levels after autophagy activation. Left panel: (A) PAECs and (B) HMEC1HaloBMPR2 were treated for 6h with rapa (10 ) and pp242 (1 ). Tubulin was used as a loading control. Representative results of at least three independent experiments are shown. Right panels: quantification of BMPR2 levels normalised to tubulin is presented as meanSEM. * <0.05; ** <0.005. BMPR2 protein levels at the plasma membrane decrease after autophagy activation. (C) Flow cytometrybased analysis of HaloBMPR2 by means of the nonpermeable Halo Alexa Fluor 488 ligand after HMEC1HaloBMPR2 were treated with pp242 (1 ) for 16h. Left panel: flow cytometry plots. Right panel: quantification of the fold Alexa Fluor 488 ligand mean fluorescence intensity upon pp242 (1 ) treatment. The data are presented as fold increases relative to untreated control. Data of four independent experiments are presented as meanSEM. ** <0.005. (D) knockdown efficacy was examined. Left panel: mRNA expression was analysed by RTqPCR. Data of three independent experiments performed in duplicates are presented as meanSEM. *** <0.001, normalised to . The data are presented as fold increases relative to the control. Right panel: western blot showing ATG7 and MAP1LC3B protein expression. GAPDH was used as a loading control. Representative results of at least three independent experiments are shown. (E) Left panel: western blot showing BMPR2 protein levels after blocking autophagy. PAECs were transfected with control or siRNA followed by 6h BafA1 treatment. Tubulin was used as a loading control. Representative results of at least three independent experiments are shown. Right panel: quantification of BMPR2 levels normalised to the loading control is presented as meanSEM. * <0.05.",yes
PMC10906230,Figure_3,oa_package/f3/a8/PMC10906230.tar.gz,"['By RNA in situ hybridization (RISH), we verified enriched expression of IGF1 and CXCL13 in the inner stroma adjacent to BPH hubs, including among hubs that were microdissected and transcriptionally profiled (exemplified by Hub-1 in , A D, and quantified in Supplemental Table 4), as well as in additional independent hubs (exemplified by Hub-7 in , E K).', 'Importantly, enriched expression did not merely reflect an enrichment of prostate fibroblasts, since interstitial prostate fibroblasts identified by expression of complement factor 7 (C7) (9) were observed in both the inner and outer stroma (, D, H, and K).', 'RISH verifies IGF1 and CXCL13 expression in inner (inductive) stroma.']","Figure 3 RISH verifies and expression in inner (inductive) stroma. ( ) RISH exemplified for a laser-microdissected hub, Hub-1. ( ) RISH exemplified for an independent hub, Hub-7. ( and ) H&E stain. Inner stroma (yellow star) and outer stroma (blue star) are indicated; scale bar is 500 m, and all figure insets are an additional 2.7 magnification. ( , , and ) expression by RISH (brown staining). ( , , and ) expression by RISH (brown staining). ( , , and ) expression (marks interstitial prostate fibroblasts) by RISH (brown staining); arrows indicate fibroblasts interspersed within the outside stroma. Note, RISH for , , and was conducted on 7 additional BPH hubs ( ).",yes
PMC8715125,Figure_2,oa_package/8a/8e/PMC8715125.tar.gz,"['The data indicate minimum degradation over a 4 h time course with 95% of [18F]F-DCP remaining intact at the 4 h time point (A).', 'Primary characterization of [18F]F-DCP compatibility with in vivo applications.', 'Given the richness of information available for this system matching general molecular features that distinguish radiation resistant cells and tumors across HNSCC, the SCC-61 and rSCC-61 cells were utilized in the initial studies to evaluate the capability of [18F]F-DCP to preferentially label radiation sensitive cells and tumors.', '5-, and 2-fold higher, respectively, in SCC-61 compared to the rSCC-61 cells (B), consistent with the higher ROS and protein sulfenylation in SCC-61 cells [10].', 'Pre-treatment of SCC-61 cells for 15 min with non-radioactive F-DCP decreased the radioactive signal by 60%, while no significant blocking of [18F]F-DCP was observed in the rSCC-61 cells (C), presumably due to the already low [18F]F-DCP signal in these cells.', 'To further provide evidence that the uptake of the [18F]F-DCP is dependent on protein oxidation, we quantified cellular [18F]F-DCP radioactivity in rSCC-61 cells treated with tBuOOH (300 M, 15 min) relative to vehicle control treatment (D).', 'The radiotracer uptake was calculated as percentage of injected dose per gram of biological specimen (tumor, organ, tissue, bone and blood; %ID/g) (E G).', 'Focusing on the tumor, the data showed 60% higher [18F]F-DCP in the radiation sensitive SCC-61 relative to the radiation resistant rSCC-61 tumors (H).', 'The results also showed good tumor-to-muscle ratio with 80% enrichment in the tumor (I) encouraging further investigations.', 'To quantify the level of radioactivity more precisely across the mouse anatomy, we conducted biodistribution studies similar to those shown in , with the difference that the analysis was performed at a single time point, 60 min post-injection of [18F]F-DCP (', '2A D) and in vivo (E I, Figs.', 'As described in the Results, the [18F]F-DCP shows very good serum stability (A) and ability to distinguish between radiation sensitive and resistant cells both in cell culture (', '2A C) and in xenograft mouse models of HNSCC (E I), and to respond to oxidative challenge (', 'The time course biodistribution analysis of [18F]F-DCP in SCC-61 and rSCC-61 xenograft mice (E I) and the single time point analysis in an extended panel of radiation sensitive and resistant HNSCC tumors (']","Fig. 2 Primary characterization of [ F]F-DCP compatibility with applications. ( ) Serum stability assay for [ F]F-DCP performed by incubating the radiotracer with human serum and quantifying by HPLC analysis over a 4h time course (n=2). ( ) cell accumulation of [ F]F-DCP in SCC-61 and rSCC-61cells after 5min, 30min and 60min of radiotracer exposure (n=3). ( ) assessment of binding specificity using blocking with non-radioactive F-DCP analog in SCC-61 and rSCC-61cells. Blocking of sulfenylated proteins was demonstrated by exposing the cells to 50x excess F-DCP, 15min prior to adding the [ F]F-DCP radiotracer for 5min, 30min, and 60min (n=3). ( ) Control experiments showing increased cellular [ F]F-DCP with oxidant treatment (tBuOOH: -butyl hydroperoxide; 300M, 15min followed by radiotracer for 30min) (n=3). ( )( ) Biodistribution of [ F]F-DCP in matched xenograft animal models of radiation response: radiation sensitive SCC-61 ( ), radiation resistant rSCC-61 ( ), and control non-tumor carrying animals ( ) (n=4 for each group). ( ) Analysis of biodistribution data shows accumulation of [ F]F-DCP in SCC-61 tumors 23 fold more than in rSCC-61 tumors, a ratio stable up to 90min post injection. ( ) Tumor to muscle ratios extracted from the biodistribution data show [ F]F-DCP accumulation in SCC-61 tumors compared to rSCC-61 tumors. The data in ( )( ) were expressed as % injected dose (ID)/mg of protein present in each well with p values0.05 considered statistically significant. Statistical analysis ( -test) was performed using Microsoft Excel and all results are displayed as meanstandard deviation. Asterisks indicate statistically significant changes [=0.05, p values of 0.010.05 (*), 0.0010.01 (**), or <0.001 (***)].",yes
PMC9738228,Figure_4,oa_package/ad/ab/PMC9738228.tar.gz,"['33 10 16) (B).', '3A-K27Q, -K27R, and -K27M mutants, we did not detect significant differences in the climbing distance (A,C).', '7928 mm/s) (E).', '2163 mm/s) (F), while we did not detect significant differences in climbing speed in the case of His3.', '3A-K27R and -K27M mutants (D,F).', 'Adult expression of K14Q mutant H3.']","Figure 4 Adult expression of K14Q mutant H3.3 ameliorates, while K14R mutant exacerbates motor phenotypes in HD flies. ( ) Vertical distances climbed during a 10 s time period by male flies co-expressing in the adult nervous system under the influence of ; with heterozygous background. Graphs show the average climbing distance in mm and the error bars represent standard error; a two-way ANOVA was used for the statistical analysis of climbing curves. ( ) In -expressing flies, climbing ability improved (genotype/height interaction = 3.79 10 ) compared with the unmodified expressing controls, while that of -expressing flies was significantly worse (genotype/height interaction = 3.33 10 ). No significant differences were observed in HD flies carrying His3.3A transgenes with mutations affecting the K9 ( ) or K27 ( ) residues. ( ) Climbing speed between the 2nd and 3rd seconds of male flies co-expressing in the adult nervous system under the influence of ; with heterozygous background. ( ) Point mutations of affecting lysine K9 do not affect the climbing speed compared with the control. ( ) Climbing speed of -expressing flies improved, while -expressing flies climbed slower compared with the control. ( ) Climbing speed of -expressing flies also improved compared with the control. Boxplots show the distribution (first quartile, median, third quartile, and 10th and 90th percentiles as whiskers). 60, * 0.05, *** 0.001, ANOVA.",yes
PMC10362807,Figure_2,oa_package/de/e4/PMC10362807.tar.gz,[],Figure 2 Extensive mesangial sclerosis of right kidney hematoxylin & eosin stain 400x magnification,yes
PMC6713087,Figure_2,oa_package/f1/cb/PMC6713087.tar.gz,"['In the asymmetrical septal variant of HCM, thickening of the basal anterior septum with subaortic bulging leading to left ventricular outflow tract obstruction (), with or without septal endocardial plaques ( friction lesion ), is observed.', '.']","Figure 2. Arrhythmic sudden death in a 23-year-old man due to hypertrophic cardiomyopathy [ ]. (A) Long axis view of the left ventricular outflow tract: the bulging of septal hypertrophy creates subaortic stenosis, which is aggravated by a fibrous plaque superimposed to the septal endocardium facing the anterior leaflet of the mitral valve; (B) Mid-apical cross-section of the same heart: note the asymmetric septal hypertrophy with reduced left ventricular cavity and the presence of white scars in the septum; (C) Fascicular disarray of the myocardium (Trichrome Heidenhain, 120); (D) Disarray of single myocytes (Trichrome Heidenhain, 30).",yes
PMC11329188,Figure_3,oa_package/ac/f8/PMC11329188.tar.gz,['Right ventricular pressure before the patent foramen ovale (PFO) closure and after the PFO closure device implanted.'],"Video 3 Transesophageal echocardiography maximal bubble study video in the left lateral position. The entire left atrium was filled with bubbles, and more bubbles were observed than in other positions.",yes
PMC2719085,Figure_2,oa_package/1f/63/PMC2719085.tar.gz,"['As in human AIP, the inflammatory infiltrates were composed predominantly of macrophages and T cells with sparing of the endocrine pancreas (fig 2A and B, respectively).', 'Further evidence for a non-fibrotic pancreatitis phenotype in Tgfbr2fspKO mice was demonstrated by the lack of expansion of nestin-positive stellate cells (fig 2C).', 'As in human AIP, endocrine function did not seem to be overtly impaired in Tgfbr2fspKO mice, as determined by insulin (fig 2D) and somatostatin expression in the islets and serum (data not shown).', 'Pancreatitis in Tgfbr2fspKO mice resembles human autoimmune pancreatitis (AIP).']","Figure 2 Pancreatitis in Tgfbr2 mice resembles human autoimmune pancreatitis (AIP). Representative immunohistochemistry stains of pancreas from Tgfbr2 mice using antibodies against (A) F4/80 (macrophage), (B) CD3 (T cells), (C) nestin (stellate cells) and (D) insulin (n=6, scale bar represents 10 m). Asterisks indicate islets and arrows indicate areas of pancreatitis. Arrowheads indicate nestin-positive cells.",yes
PMC6660770,Figure_2,oa_package/e4/d9/PMC6660770.tar.gz,"['Moreover, using a filtration method with spin filters, presented in , and SEC, we confirm that such isolation of lipoproteins enriched with monomeric SN does not affect the interaction between monomeric SN and lipoproteins (\nPancreatic ductal adenocarcinomas.']",Fig. 2 Pancreatic ductal adenocarcinomas. Axial ( ) and precontrast ( ) pancreatic parenchymal phase and ( ) venous phase image showing an ill-defined hypodense hypoattenuating lesion arising from the tail of pancreas ( ). Well-defined peripherally enhancing hypodense liver lesions are seen ( ) suggestive of liver metastases.,yes
PMC5530091,Figure_2,oa_package/73/38/PMC5530091.tar.gz,"[' summarizes the location of retroperitoneal paragangliomas in all 34 patients.', '.']","Figure 2. Locations of retroperitoneal paragangliomas in 34 patients in the coronal plane. The number corresponds to the patient number ( ) and the circle around the number represents the size of the tumor. Retroperitoneal paragangliomas were primarily located close to the renal arteries and veins, surrounding the abdominal aorta and inferior vena cava.",yes
PMC9613377,Figure_2,oa_package/2c/2f/PMC9613377.tar.gz,"['0 cm from ostium3) 90% occlusion, distally, at the origin of the posterior coronary arteryThe shows the gross images of the left circulation with marked aneurysmal dilation and severe coronary artery atherosclerosis.', '\nA Fresh magnified gross view of the serial sections of the left anterior descending coronary artery (base of the heart and mid proximal left anterior descending coronary artery in the left side of the photo and apex and mid distal left anterior descending coronary artery in the right side of the photo) with variable degrees of coronary artery aneurysms up to 1.']","Figure 2 Fresh magnified gross view of the serial sections of the left anterior descending coronary artery (base of the heart and mid proximal left anterior descending coronary artery in the left side of the photo and apex and mid distal left anterior descending coronary artery in the right side of the photo) with variable degrees of coronary artery aneurysms up to 1.8 cm in diameter (scale bar= 1cm); 1 x view of microscopic slides showing dilation of the circumference of the left anterior descending coronary artery (scale bar= 1cm); and 10 x and 40 x view of Verhoeff-van Gieson (VVG) stain of the left anterior coronary artery showing destruction of the tunica media with loss of smooth muscle, disarray pale degenerated areas, and elastic media fragmentation.",yes
PMC10146901,Figure_4,oa_package/bb/ee/PMC10146901.tar.gz,"[' shows sample images from a patient with a discrepancy between the original report and tumor board review.', 'Images from a 71 year-old man with IDH wild-type glioblastoma.']","Figure 4 Images from a 71 year-old man with IDH wild-type glioblastoma. Images from 4/23/2018 (middle row, white box) were reviewed at tumor board. Compared with prior study (top row), both FLAIR (white arrow) and enhancing tumor volume were increasing (yellow arrows). While the radiology report called this study a 3b, the tumor board presenter and the tumor board consensus felt the increase was sufficient to score it as disease progression (BT-RADS 4). Subsequent follow-up (bottom row) showed further increase (arrows) and the patient underwent re-irradiation of recurrent disease.",yes
PMC7734283,Figure_3,oa_package/18/df/PMC7734283.tar.gz,"['However, the percentage of CD45RA-FoxP3lo non-suppressive cells was significantly higher in SLE than the control (A).', 'IFN- producing-T-bet+Foxp3lo non-suppressive cells were increased, whereas IFN- nonproducing-T-bet+Foxp3hi activated-Treg cells were decreased in patients with SLE.', 'Phosphorylation of mTOR was significantly correlated with T-bet expression in CXCR3loT-bethiCD4+ cells, but not in total CD4+ cells (D).', 'In the present study, CD45RA-Foxp3lo non-suppressive T cells were increased in patients with SLE as previously reported (A).']","Figure 3 IFN- producing-T-bet Foxp3 non-suppressive cells were increased, whereas IFN- nonproducing-T-bet Foxp3 activated-T cells were decreased in patients with SLE. PBMCs were obtained from 16 HDs and 18 SLE patients, and CD3 CD4 T cells were gated. Expression of Foxp3 and CD45RA in CD4 T cells were analyzed by intracellular staining using flow cytometry and shown in the representative dot plots (left panels) and scatter plots of percentage of CD45RA Foxp3 cells (nave-Treg), CD45RA Foxp3 cells (non-suppressive cells), and CD45RA Foxp3 cells (activated-Treg) (right panels). Double-staining of T-bet and IFN- expression in CD4 T cells and gated in CD45RA Foxp3 cells (nave-Treg), CD45RA Foxp3 cells (non-suppressive cells) and CD45RA Foxp3 cells (activated-Treg) in HDs and SLE patients. Data are representative dot plots (left panels) and scatter plots of percentage of T-bet IFN cells among Foxp3 CD45RA CD4 cells (right panel). Double-staining of T-bet and CXCR3 expression in CD4 T cells and gated in CD45RA Foxp3 cells (activated-Treg) in HDs and SLE patients. Data are representative dot plots (left panels) and scatter plots of percentage of CXCR3 cells among CD4 CD45RA Foxp3 cells (right panel). The MFI/isotype MFI of mTOR phosphorylation in CD4 T cells from HDs and SLE patients was analyzed by flow cytometry and shown in the scatter plots. Right panel: Correlation between T-bet/isotype MFI and p-mTOR/isotype MFI in CD4 T cells and CD4 CXCR3 T-bet cells from SLE patients. Data are mean SD. Data are mean SD. *p < 0.05, ****p < 0.0001. n.s., not significant.",yes
PMC4640715,Figure_5,oa_package/24/e5/PMC4640715.tar.gz,"['The overall nephropathy score was not remarkably different following macrophage depletion (E).', 'A minimal decrease in glomerular sclerosis was observed in KO+CL mice when compared to KO mice, however there was no reduction when compared with the KO+PBSL mice (E).', 'g005Macrophage depletion did not improve renal pathology in Col4a3KO mice.']",10.1371/journal.pone.0141231.g005,yes
PMC6883876,Figure_6,oa_package/9f/20/PMC6883876.tar.gz,['Defect was packed by Gauze pack intraorally and closure done [].'],Figure 6 Immediate postoperative,yes
PMC9887356,Figure_2,oa_package/52/b3/PMC9887356.tar.gz,"[' illustrates the staining results of MMR-deficient tumours.', 'Whole slides of immunohistochemical staining of MMR-proteins MLH1, MSH2, MSH6 and PMS2.']","Figure 2 Whole slides of immunohistochemical staining of MMR-proteins MLH1, MSH2, MSH6 and PMS2. Note the strong staining of inflammatory and stromal cells and additionally the strong nuclear expression of tumour cells for MLH1 and PMS2. In contrast, immune and stromal cells are strongly stained (internal positive control), but the tumour cells are showing a nuclear loss of expression for MSH2 and MSH6. MLH1, mutL homologue 1; MMR, mismatch repair; MSH2, mutS homologue2; MSH6, mutS homologue 6; PMS2; PMS1 homologue 2.",yes
PMC9579545,Figure_4,oa_package/54/90/PMC9579545.tar.gz,"[' 4a).', ' 4b).', ' 4c).', ' 4d), H E staining of fixed lung lobes (', ' 4e and ', ' 4f and Table S2) demonstrated that juvenile and adult hamsters with early IFN- 2b treatment had significantly less lung injury than those without treatment.', ' 4g i), suggesting strong suppression of viral replication and accelerated viral clearance induced by IFN treatment.', 'Early IFN- 2b treatment efficiently suppressed SARS-CoV-2 Omicron BA.', '001; ns nonsignificant)DiscussionThe evolving SARS-CoV-2 mutates to adapt to its hosts, resulting in the emergence of new variant strains with varied disease outcomes after infection.']","Fig. 4 Early IFN-2b treatment efficiently suppressed SARS-CoV-2 Omicron BA.1 replication and lung pathogenesis. Juvenile and aged male hamsters were intranasally inoculated with 110 PFU of SARS-CoV-2 Omicron BA.1 and then administered three doses of recombinant IFN-2b treatment at 24, 25, and 26h post-infection. The body weight changes of SARS-CoV-2 Omicron BA.1-infected ( ) juvenile, ( ) adult and ( ) aged hamsters and hamsters with early IFN-2b treatment were measured from 0 to 5 dpi ( =4/group). All hamsters were euthanized at 5 dpi. Gross lung images, representative H&E staining of lung lobes and comprehensive pathological scores are shown (for each group, 16 lung lobes collected from four hamsters were evaluated). The viral RNA loads in respiratory tract organs, including the ( ) turbinates, ( ) trachea and ( ) lungs, were measured by RTPCR ( =4/group). We analyzed the differences between the hamsters with and without early IFN-2b treatment in each age group. Significance was calculated using an unpaired two-tailed test (* <0.05; ** <0.01; *** <0.001; ns nonsignificant)",yes
PMC11294153,Figure_6,oa_package/83/8e/PMC11294153.tar.gz,"['Exposure to WD promoted a gradual increase in body weight in both CT and EC-Myc OE mice (A).', '035) (B).', '028) (C).', '001) (D).']","FIGURE 6 Gross phenotype analysis of control and endothelial c-Myc overexpression mice under exposure to western diet. Longitudinal analysis of body weight and weight gain for a total period of 10 weeks. White and black symbols represent animals exposed to control and western diet, respectively. Analysis of body weight at 5- and 10-weeks endpoints. Quantification of visceral (VAT), epididymal (EAT) and brown (BAT) adipose tissue mass at 10-weeks endpoint. Quantification of systemic leptin levels at 10-weeks endpoint. In A, results are represented as mean standard error. In all other graphs, dots represent individual animals, filled circles represent the mean standard deviation. CT, Control; OE, Endothelial c-Myc overexpression; CTD, control diet ( = 1422); WD, western diet ( = 2231); ns, non-significant. (* < 0.05, *** < 0.001).",yes
PMC8994011,Figure_2,oa_package/1d/05/PMC8994011.tar.gz,"[' 2) and treatment.', ' Western blotting is the more specific test, used as a confirmatory test in addition to ELISA and IHA (sensitivity varies according to CE stage)Schematic structure of a cystic echinococcosis cystAlveolar echinococcosisAlveolar echinococcosis (AE) is a rare but invasive disease, caused by infection with the larval stage (metacestode) of the parasite Echinococcus multilocularis.']","Fig. 2 Role of imaging in the positive diagnosis of CE and AE. First-line tests generally comprise EgHF-ELISA or indirect hemagglutination. The confirmation test in combination with the Em2-and rec-Em18-ELISA enables diagnosis with sensitivity of almost 100%. Western blotting is the more specific test, used as a confirmatory test in addition to ELISA and IHA (sensitivity varies according to CE stage)",yes
PMC7722332,Figure_1,oa_package/cb/dc/PMC7722332.tar.gz,"['1a, b) was significantly higher in Nrf2tKO mice on the 3rd day after muscle damage.', '1c) was increased in Nrf2tKO animals on day 1 after injection, a statistically significant difference between WT and Nrf2tKO mice was not evident.', '1d) was significantly elevated in mice of both genotypes, and additionally, it was much higher in Nrf2tKO animals in comparison to WT on the 1st day after CTX injection.', '1e) and mRNA level of Hmox1 (', '1f), Il1b (', '1g) and Il6 (', '1h) on the 1st day after CTX injection in both genotypes.', '1i, j).', 'CTX-induced injury in GM of WT and Nrf2tKO animals.', 'The scale bars represent 100 mMuscle regeneration is not affected in the absence of transcriptionally active Nrf2 following CTX-induced injuryTo assess the role of Nrf2 during muscle regeneration following the acute muscle injury caused by CTX injection, we examined the mRNA level of Myh3 and the number of eMyHC+ myofibers.', '11a).', '11b, c, respectively).', '1Muscle degeneration and inflammation of WT, Nrf2tKO, mdx, and Nrf2tKOmdx mice after the long-term treadmill.', 'The scale bars represent 100 mThe number of necrotic fibers (', '11d, e) was significantly higher in GM of mdx mice in comparison to WT, and interestingly, it was further increased in Nrf2tKOmdx animals in comparison to mdx (', '11d, e).', '11f, g).', '11h, i), but not in the diaphragm (', '11j, k).', '12a, b) nor the diaphragm (', '12c, d).', '2Fibrosis in WT, Nrf2tKO, mdx, and Nrf2tKOmdx hind limb muscle and diaphragm after the long-term treadmill.', '0001, one-way ANOVA with Tukey s post hoc test; the scale bars represent 100 mInflammation and fibrosis are not affected in 24-week-old dystrophic mice by the lack of transcriptional activity of Nrf2To assess the role of Nrf2 transcriptional deficiency on inflammation and fibrosis processes in old mice, we have performed H E and Masson s trichrome staining on GM and diaphragm of 24-week-old animals.', '13a, b) and diaphragm (', '13c, d) muscles in mdx mice in comparison to their healthy counterparts; however, it was not further changed by the lack of transcriptionally active Nrf2.', '13e, f) and diaphragm (', '13g, h) of dystrophic mice was demonstrated, but it was not altered by the lack of Nrf2 transcriptional activity in both muscles.', '3Inflammation and fibrosis in muscles of non-exercised 24-week-old WT, Nrf2tKO, mdx, and Nrf2tKOmdx mice.', '0001, one-way ANOVA with Tukey s post hoc test; the scale bars represent 100 mDiscussionDMD is still an incurable disease with very limited treatment possibilities, including corticosteroids [38].', 'pdf"">Additional file 1: Supplementary .']","Fig. 1 CTX-induced injury in GM of WT and Nrf2 animals. ( ) Representative photos and ( ) semi-quantitative assessment of GM damage based on H&E staining; = 4-6. The activity of ( ) CK and ( ) LDH in plasma; activity assay; = 4-5. ( ) MCP-1 protein level in GM, Luminex ; = 4-5. ( ) , ( ) , ( ) level in GM; qRT-PCR; = 4-6. ( ) Representative photos of microscopic assessment of myofiber necrosis in GM and ( ) quantification of the staining; = 4. The data are presented as mean +/ SEM; * 0.05; ** 0.01; *** 0.001; **** 0.0001 vs. day 0; # 0.05; ### 0.001 vs. WT, one-way ANOVA with Tukeys post hoc test. The scale bars represent 100m",yes
PMC9583263,Figure_2,oa_package/a9/62/PMC9583263.tar.gz,[],"Figure2 H7N9 avian influenza viral localisation in tissues of 16 week-old chickens inoculated the ONO route. Kidney (Chicken 11), showing viral antigen in renal tubules (arrowheads). Viral infection is also associated in some regions with localised necrosis of renal interstitium and tubules (*). Kidney (Chicken 11), higher magnification of the same tissue, showing viral antigen in the epithelium of the tubules. While some tubules appear healthy (arrows), others are distended with necrotic material (arrowheads). Heart (Chicken 11), showing that viral antigen is mainly within capillary endothelium (arrowheads) and in one cardiomyocyte (arrow). Heart (Chicken 11), higher magnification of the same tissue, showing detail of viral antigen in capillaries. Enucleated erythrocytes can be seen lined up within some of the capillaries. Lung (Chicken 5), showing viral antigen within single cells, presumed to be macrophages, within the lung parenchyma. Viral antigen is present in the yolk material (*) and inflammatory exudate within the airsac overlying the pancreas (bottom right of the image) and in the airsac membrane overlying the inflamed serosal surface of the duodenum (arrow) (Chicken 11). Immunohistochemistry for influenza nucleoprotein (red-brown pigment), counterstained with haematoxylin.",yes
PMC3611060,Figure_3,oa_package/14/98/PMC3611060.tar.gz,"['EMG and NCV studies indicated a left ulnar neuropathy below the elbow level, and ultrasound scanning of the left ulnar nerve along its expected course from the elbow level distally was performed, revealing a mass lesion surrounding the left ulnar nerve at the mid-forearm level (A, B, G).', '5 cm tubular enhancing mass along the left ulnar nerve (C-F), consistent with neurogenic tumor (neurofibroma or schwannoma).', 'Transverse 13-7 MHz ultrasound image over the volar side of the mid forearm.']","Fig. 3 Transverse 13-7 MHz ultrasound image over the volar side of the mid forearm. Right (A) and left (B) : The intact ulnar nerve (arrowheads) and ulnar artery (black arrows) are seen on the right side. On the left side, a hypoechogenic mass is detected instead of the ulnar nerve. C and D are transverse T1-weighted magnetic resonance (MR) images from the same site. An enhancing round-shaped mass is seen near the ulnar artery. Sagittal T1-weighted MR images (E and F) and longitudinal ultrasound image (G) show the whole length of the mass lesion (white arrows) and they show a corresponding contour with each other. US : ulnar shaft.",yes
PMC6296098,Figure_1,oa_package/23/28/PMC6296098.tar.gz,"[' 1).', 'Intrinsic and extrinsic apoptosis pathway.', 'Adapted and partially modified from [23]Need for epigenetic analysis on the effect of butyric acid on HGFsButyric acid is known to act as an HDAC inhibitor [12, 26].']",Fig. 1 Intrinsic and extrinsic apoptosis pathway. Adapted and partially modified from [ ],yes
PMC6887874,Figure_2,oa_package/47/3b/PMC6887874.tar.gz,"[' summarized the selected images of 2 patients (A B) demonstrating abnormal HSG findings confirmed on subsequent MRI.', 'Selected images of 2 patients (A B) demonstrating abnormal hysterosalpingography (HSG) findings confirmed on subsequent MRI: A1- Flattened contour of uterine cavity is seen on HSG in a secondary infertility patient.', 'Differentiation of certain entities such as septate and bicornuate uterus can be sometimes difficult on HSG study, and may require further imaging such as 3-dimensional sonography or MRI for confirmation ().']",Figure 2 Selected images of 2 patients (A & B) demonstrating abnormal hysterosalpingography (HSG) findings confirmed on subsequent MRI: A1- Flattened contour of uterine cavity is seen on HSG in a secondary infertility patient. A2- Selected sagittal reformat CT image of same patient showing a large degenerated fundal fibroid (star) compressing upon the uterine cavity (arrow). B1- Suspicion of septate uterus with two uterine cavities (arrow)on HSG in a primary infertility patient. B2- Selected T2W coronal reformat image of the same patient showing completely septate uterus with myometrial tissue between the 2 uterine horns (arrow).,yes
PMC4479763,Figure_1,oa_package/eb/b7/PMC4479763.tar.gz,"['Palatal examination disclosed a localized circumscribed swelling of the marginal gingiva, which appeared cyanotic (a).', 'Further, on periodontal probing, a 10 mm pocket could be probed on the mid palatal aspect of the root (b).', 'Intraoral peri-apical radiograph of the tooth showed a lateral peri-apical radiolucency and a dark radiolucent line superimposed over the canal space (c).', 'Gutta-percha tracing of the periodontal pocket pointed towards the periapical area (d).', 'Preoperative images, (a) Clinical palatal view showing swelling of marginal gingiva, (b) probing depth of 10 mm seen at the cingulum, (c) intra oral periapical (IOPA) radiograph showing lateral peri apical radiolucency with a thin radiolucent line superimposed on the canal space and (d) IOPA radiograph showing gutta percha cone tracing.']","Figure 1 Preoperative images, (a) Clinical palatal view showing swelling of marginal gingiva, (b) probing depth of 10 mm seen at the cingulum, (c) intra oral periapical (IOPA) radiograph showing lateral peri apical radiolucency with a thin radiolucent line superimposed on the canal space and (d) IOPA radiograph showing gutta percha cone tracing.",yes
PMC10705747,Figure_3,oa_package/16/fe/PMC10705747.tar.gz,"['Our data indicate that CBD counteracts the A 1 42-induced decline in these mRNA levels in the mouse cerebral cortex (A E).', 'However, no recovery of Camk2 levels was observed following CBD treatment (F), suggesting that CBD may possess specific synaptic repair effects.', 'In A 1 42-induced mouse models, their expression was significantly reduced, but CBD treatment markedly elevated their levels (G,H).', 'CBD s neuroprotective role and synaptic dysfunction alleviation in A 1 42-induced mice.']",Figure 3 CBDs neuroprotective role and synaptic dysfunction alleviation in A -induced mice. ( ) Quantified image showing the mRNA expression level of Glua1. ( ) Quantified image showing the mRNA expression level of Glua2. ( ) Quantified image showing the mRNA expression level of CamKII. ( ) Quantified image showing the mRNA expression level of CamKII. ( ) Quantified image showing the mRNA expression level of Syp. ( ) Quantified image showing the mRNA expression level of Dlg4. ( ) Quantified image showing the mRNA expression level of BDNF. ( ) Quantified image showing the mRNA expression level of GDNF. Data are shown as mean SEM ( = 4). * < 0.05 vs. sham + Veh group; < 0.05 vs. AD + Veh group.,yes
PMC4349663,Figure_4,oa_package/61/ec/PMC4349663.tar.gz,"['05) [ 4A, B].', '05) [ 4B].', 'The levels of serum anti-ANT autoantibody levels in the IL-21 mAb, isotype control and PBS groups were elevated dramatically, compared with those in the normal group, especially in the isotype control and PBS groups [ 4C].', '05) [ 4C], which indicated that blockade of IL-21 can reduced circulating level of anti-ANT autoantibodies.', '\nTfh cell proportions and circulating level of anti-ANT autoantibodies decreased after IL-21 inhibition.', 'Positive correlation of Tfh cell proportions and IL-21 with levels of Anti-ANT autoantibodyFCM results suggested that Tfh cells and IL-21 increased in VMC mice, and blockade of IL-21 reduced Tfh cell proportions.']","Figure 4 . The percentages of Tfh cells in each groups investigated by flow cytometry. Tfh subsets were gated with CXCR5 ICOS /CD4 cells. Numbers in upper right quadrants represents an average number of positive cells in each group. All staining samples were restimulated with PMA/ionomycin for 4h before analysis. . The results of the Tfh cells statistical analysis. The percentages of Tfh cells in IL-21mAb group was fewer than control and PBS group. <0.05, versus normal group. <0.05, versus isotype control mice. <0.05, versus PBS group mice. . Anti-ANT autoantibody levels in the different groups. <0.05, versus normal group. <0.05, versus isotype control mice. <0.05, versus PBS group mice.",yes
PMC11281792,Figure_7,oa_package/44/2a/PMC11281792.tar.gz,[],"Figure 6. Altered pathways in senescence-enriched COPD SAF. Samples from FSC FITC () and FSC FITC () sorted COPD SAF were analyzed by RNA sequencing ( = 6 donors). : volcano plot of differentially expressed genes in sorted senescence-enriched populations. Labeled are some of the top genes most upregulated and downregulated in senescence-enriched SAF. Horizontal dashed line shows adj = 0.05. Vertical dashed lines show a fold change of 1.5 ( = 6 donors). : summary of top 5 upregulated and downregulated genes based on Log2 fold change. : ingenuity pathway analysis of significantly enriched canonical pathways based on differentially expressed genes from RNA-seq data ( = 6 donors). : enrichment for previously published gene sets altered in senescence in this RNA dataset. : differentially expressed genes involved in the fibrosis pathway enrichment. : expression of fibrosis-related genes , , and in sorted COPD SAF ( = 6 donors). Data are represented as individual data points and/or mean. Data were analyzed using a Wilcoxon test. * < 0.05. COPD, chronic obstructive pulmonary disease; SAF, small airway fibroblast.",yes
PMC9765427,Figure_6,oa_package/2f/88/PMC9765427.tar.gz,"['In stationary-phase cells, XOG1 and ENG1 transcript levels were decreased following Mig1/2 inactivation, suggesting that Mig1 and Mig2 are required for normal levels of expression under these conditions (', ' 6a and b).', 'Sin3 inactivation did not affect XOG1 and ENG1 transcript levels dramatically, but the loss of PKA (tpk1 tpk2 ) reduced XOG1 transcript levels in stationary-phase cells and increased XOG1 and ENG1 levels in exponential-phase cells, whether or not lactate was present (', ' 6a and b).', 'FIG 6Expression of genes encoding -1,3-glucan shaving enzymes is modulated by PKA, Mig1/2, and Sin3.', 'For example, the inactivation of PKA led to elevated XOG1 mRNA levels (', 'The levels of secreted Xog1 were dramatically reduced in tpk1 tpk2 cells and elevated in sin3 cells (', 'The levels of secreted Eng1 were reduced in mig1 mig2 cells (', 'Therefore, the levels of Xog1 and Eng1 in the secretome correlated with their roles in -1,3-glucan masking, but their transcript levels did not (', ' 6a and b).', 'For example, Xog1 levels in the secretome were elevated in sin3 cells (', 'However, the inactivation of PKA, Sin3, and Mig1/2 had differential effects on XOG1 and ENG1 transcript levels (', ' 6a and b), the levels of these -1,3-glucanases in the secretome (', 'Rather, Sin3 appears to regulate Xog1, while Eng1 seems to be modulated by Mig1/2 (', ' 6a and b).', 'PKA regulates growth, stress resistance, morphogenesis, and candidalysin production (73, 75, 99 101) as well as -1,3-glucanase levels in the secretome (', 'Mig1 and Mig2 influence hyphal development and biofilm formation and regulate carbon metabolism (50), in addition to Eng1 levels (', ' 7c), as well as Xog1 levels (']","FIG6 Expression of genes encoding -1,3-glucan shaving enzymes is modulated by PKA, Mig1/2, and Sin3. (a and b) (a) and (b) transcript levels measured relative to the and internal controls. The following strains were grown overnight to stationary phase on GYNB (gray) or for 60min on GYNB (Glu; red) or GYNB containing lactate (GluLac; blue): WTm (SN250), mutant, mutant, WTt (SN152HLA), and mutant. (c) Relative levels of Xog1 and Eng1 in the secretome as quantified by LC-MS/MS. Cells were grown in GYNB plus lactate for 5 h. The level of the target protein in the mutant secretome is divided by the level in the secretome of the corresponding isogenic parental strain; strains include WTm (SN250), mutant, mutant, WTt (SN152HLA), and mutant. The data, which represent means of results from three replicate experiments, were analyzed using two-way ANOVA with Tukeys multiple-comparison test (*, <0.05; **, <0.01; ***, <0.001).",yes
PMC6262067,Figure_8,oa_package/1d/ef/PMC6262067.tar.gz,"['The transcystic portal is created by direct visualization with a localizing needle () placed percutaneously from the most superficial location of the cyst, preplanned with the MRI scan (Fig 9).', 'Arthroscopic image viewed from the posteromedial portal of needle localization for a transcystic portal.']","Fig 8 Arthroscopic image viewed from the posteromedial portal of needle localization for a transcystic portal. This is placed percutaneously at the most superficial location of the cyst, which is preplanned with the magnetic resonance imaging scan. This portal is often directly posterior, and transillumination may be helpful to locate this portal and to avoid superficial calf veins. The cyst walls can be removed using a shaver via this portal.",yes
PMC6581016,Figure_7,oa_package/8f/b5/PMC6581016.tar.gz,"['The axons and synaptic terminals of MB neurons form a pair of bilaterally arranged and symmetrically lobed structures which show low basal Ca2+ fluorescence levels (A left panel) which, in response to depolarizing high [K+] saline, show a large peak in Ca2+ fluorescence levels (A right panel).', 'The increase in neuronal activity can be expressed as the relative fluorescence change ( F/F0) over time (B) showing that high K+ causes a rapid peak Ca2+ influx that returns to baseline after washing.', 'MB wide overexpression of human mutant APP (A 42), MAPT (0N4R Tau) or mis-expression of Ank2 all caused a similar decrease in peak Ca2+ influx of memory neurons (C), suggesting a reduction in neuronal excitability.']","FIGURE 7 The effect of expression of human mutant (A42), (Tau 0N4R), and on activation of mushroom body neurons. Exemplary control 25 day old fly MB expressing the calcium reporter GCaMP6f shows a big increase in relative fluorescence in response to elevated KCl (100 mM, bath-applied). Images show the same brain before (left) and during (right) KCl application; scale bar 50 m. The increase in neuronal activity is expressed as the relative change in fluorescence (F/F0) over time showing that high KCl (indicated by bar) causes peak Ca influx that returns to baseline after washing. Quantitative analysis of the maximal response for the indicated genotypes shows a reduced responsiveness of all mutants. Data was analyzed with one-way ANOVA with Tukeys and error bars are standard deviation, 5 brains.",yes
PMC10730449,Figure_10,oa_package/b5/cb/PMC10730449.tar.gz,[],FIGURE 10 (a) Contrast-enhanced CT demonstrates preferential enhancement of the central liver of liver (white arrowhead) in a patient with Budd-Chiari syndrome. (b) Sagittal CT abdomen showing narrowing of the infrahepatic inferior vena cava (white arrowhead) and an enlarged caudate. (c) Venogram confirms the diagnosis. (d) Venogram placement of porto-systemic venous shunt.,yes
PMC5630242,Figure_3,oa_package/c1/db/PMC5630242.tar.gz,"['Nodular SCIS is located in the dermis and/or subcutis with or without surface epithelial involvement, has a multilobulated (A C) or solid nodular configuration (A C).', '](A) Low power view of sebaceous carcinoma in situ, multilobulated nodular type (H E stain).']","Figure 3 Low power view of sebaceous carcinoma in situ, multilobulated nodular type (H&E stain). Intermediate power view of sebaceous carcinoma in situ, multilobulated nodular type (H&E stain). High power view of sebaceous carcinoma in situ, multilobulated nodular type (H&E stain). [Copyright: 2017 Komforti et al.]",yes
PMC11634889,Figure_2,oa_package/cc/77/PMC11634889.tar.gz,"['2a).', '2a, b).', '2c, d).', '2e, f), but also after probing with anti-mASYN (', '2g, h).', '2i, j).', 'AAV-induced hASYN overexpression in the dMO.', 'Overexpression-induced ASYN aggregation was assessed in medullary tissue sections collected from control and L444P/wt mice at 6 weeks post-AAV treatment and stained with combinations of 3 different antibodies.', '2a).']","Fig. 2 AAV-induced hASYN overexpression in the dMO. All mice received an intravagal injection of hASYN-delivering AAVs and were sacrificed 6 weeks later. , MO tissue sections were stained with a specific antibody against hASYN. Representative brightfield images of the dMO from a control (Ctr) and an L444P mutant (Tg) mouse. Boxes in the low-magnification images encompass areas of the DMnX that are also shown at higher magnification. Scale bars: 250m (low magnification) and 20m (higher magnification) ( ). The number of hASYN-immunoreactive (IR) neurons was counted in sections of the left (ipsilateral to the AAV injection) DMnX of control ( =5) and L444P mutant ( =5) mice ( ). , Representative fluorescent images of neurons in the left DMnX stained with anti-hASYN. Scale bar: 20m ( ). Fluorescent intensity (expressed as arbitrary units) of hASYN-positive neurons in the left DMnX of control ( =6) and L444P mutant ( =8) mice ( ). , Western blots from homogenates of the dMO showing immunoreactivity specific for hASYN ( ), mASYN ( ), total (human plus rodent) ASYN (tASYN) ( ) and -tubulin. Semiquantitative analyses of band optical densities. Analyses were made in samples from control and L444P mutant mice ( =5/group). Data were calculated as hASYN/-tubulin ( ), mASYN/-tubulin ( ) and tASYN/-tubulin ( ) ratios and expressed as percentage of the mean value in the respective control group. Plots show median, upper and lower quartiles, and maximum and minimum as whiskers.",yes
PMC3352898,Figure_2,oa_package/ec/68/PMC3352898.tar.gz,"['5X was higher in the wild-type than in each of the mutants (A).', 'While the number of genes altered in the D380N (2,237) and K423E (2,283) mutations were pretty similar to those changed by the stop mutation, the mutation R342K induced only 803 changes in the HTM transcriptome (A).', 'The highest difference was observed in the R342K mutant, which had a higher number of downregulated genes (274 genes up- and 529 genes downregulated) (A).', 'g002Wild-type MYOC and different MYOC mutants alter different gene numbers on the trabecular meshwork transcriptome.', 'g002""/>When comparing the number of genes altered in the mutants with those altered in the wild-type, we found that the stop mutation Q386X had the highest difference with a total of 6,716 (3,342 up and 3,374 down) (B).', 'The mutation R342K, whose absolute number of altered genes is the lowest of the mutants studied (), contained 542 non overlapping genes, which amounts to a still high percentage of the total (56 ) (A).']",10.1371/journal.pone.0036301.g002,yes
PMC7118982,Figure_4,oa_package/ea/de/PMC7118982.tar.gz,"['A functional PDZ-domain binding motif at the C-terminal end of the HCoV-OC43 E protein is critical for efficient virus production and spread in neuronal cells.', 'To investigate whether the ability to infect susceptible cells, replicate and disseminate is affected by the putative C-terminal PBM in the context of the CNS, LA-N-5 or mixed primary cultures of murine CNS cells were infected with single or double mutant PBM viruses and viral titers and propagation were analyzed.', 'In LA-N-5 cells, after 18 hpi, the titers of rOC/E-PBMD82A-V84A were significantly decreased in the cell-free and cell-associated virus fraction compared to single PBM mutants or reference viruses and total infectious virus titers of the double mutant was severely altered over 72 h (B).', 'This trend was exacerbated in primary mixed murine CNS cultures, which showed no detection of infectious rOC/E-PBMD82A-V84A compared to single mutant PBM and reference viruses (C).', 'Immunofluorescence analysis indicated that cells could be infected by all viruses but we detected a significant difference in propagation for both LA-N-5 cells (D, S2A) and primary mixed murine CNS cultures ( S2B), showing a significantly reduced propagation for the double PBM mutant rOC/E-PBMD82A-V84A.', 'Through the replacement of the key amino acids of this motif by alanines, we demonstrated its importance in infectious virion production in the epithelial and neuronal cells tested ().', 'rOC/E-PBMD82A-V84Ais able to produce infectious virions in the brain and to propagate to the spinal cord after an infection at a higher dose.', '\nAcknowledgementsWe thank Jessie Tremblay for excellent technical assistance with confocal microscopy.']","Fig. 4 A functional PDZ-domain binding motif at the C-terminal end of the HCoV-OC43 E protein is critical for efficient virus production and spread in neuronal cells. (A) Production of infectious virus after transfection of various E protein mutants with fully or partially abrogated predicted PBM after transfection in BHK-21 cells (BHK 0) and amplification on HRT-18 cells (HRT 1). The results show a representative experiment. Cell-free and cell-associated infectious virus production were determined at indicated timepoints after infection at an MOI of 0.05 on (B) LA-N-5 cells and (C) mixed primary cultures of mouse CNS cells. The results show a representative experiment. Statistical significance was tested at 72hpi (** P < 0.01; *** P < 0.001). (D) The percentage of LA-N-5 cells infected by the transmembrane mutant (representative of viral propagation) was quantified by immunofluorescence and cell profiler software and compared to the reference virus over 72h post-infection. LOD, limit of detection.",yes
PMC7615119,Figure_11,oa_package/60/36/PMC7615119.tar.gz,[],"Fig.1 deletion leads to EC activation and proliferation only in some vascular beds. , , Notch1 signaling activity (cleaved Val1744 N1ICD) in quiescent endothelium (DAPI Endomucin , abbreviated as EMCN). , Schematic representation to illustrate the bulk RNA-seq experiment performed with adult ECs isolated by FACS. , Heatmap with RNA-seq reads per kilobase per million mapped reads (RPKM). , Experimental layout for the inducible deletion of in Cdh5 ECs with , Expression of Dll4 protein in CD31 EMCN vessels. , , deletion significantly reduces Notch signaling activity (cleaved Val1744 N1ICD) in all quiescent vascular beds. In brain micrographs, white arrowheads indicate ECs and yellow arrowheads indicate non-ECs. Note that whereas N1ICD is maintained in non-ECs, most N1ICD signal disappears from the ECs in brains. , Schematic representation to illustrate the bulk RNA-seq experiment performed with adult ECs. Below, a heatmap showing the relative expression of all Notch pathway components and canonical target genes in control and mutant ECs. , Unsupervised hierarchical clustering showing stronger gene expression changes in liver ECs compared with the other organs. -score lcpm, -score of the logarithmic counts per million. , Unsupervised hierarchical clustering showing strong upregulation of Myc target genes in liver ECs compared with the other organs. - , deletion results in increased EC proliferation (Ki67 ERG cells) in some organs but not others. , 3D reconstruction images from thick vibratome sections show vessel (CD31 EMCN ) enlargement in heart and liver but not in brain. Data are presented as mean values s.d. For statistics, see Source Data File 1. Scale bars, 100 m.",yes
PMC11604286,Figure_2,oa_package/46/c2/PMC11604286.tar.gz,"['DSA with subtraction imaging of the left kidneyDigital subtraction angiography (DSA) with subtraction imaging reveals multiple areas of extravasation in the left kidney, denoted by the arrows.']","Figure 2 DSA with subtraction imaging of the left kidney Digital subtraction angiography (DSA) with subtraction imaging reveals multiple areas of extravasation in the left kidney, denoted by the arrows. Areas of pseudoaneurysm formation with possible extravasation (arrowheads).",yes
PMC7270622,Figure_4,oa_package/e0/5a/PMC7270622.tar.gz,"['The orientation of septa was recorded as having a mediolateral or antero-posterior direction ().', '200-fig4""/>Statistical analysisStatistical analysis was performed using the IBM SPSS for Windows, version 20 (IBM Corp, Armonk, NY, USA).']",Figure 3. Axial CBCT views of two patients showing bilateral complete septa (left) and bilateral partial septa (right).,yes
PMC2627904,Figure_3,oa_package/3d/cd/PMC2627904.tar.gz,"['g002""/>HemorrhagesThere were significantly more hemorrhages in CM patients than in patients with non-malarial disease (Table 1)(A).', 'g003A.', 'The retinal hemorrhages were usually located in the inner layers, but when numerous and large, involved all layers and were associated with subretinal hemorrhage and a shallow retinal detachment (B).']",10.1371/journal.pone.0004317.g003,yes
PMC11526661,Figure_6,oa_package/52/13/PMC11526661.tar.gz,"[' 6A).', ' 6B).', ' 6B; Supplementary Table 19).', ' 6C).', ' 6D).', ' 6E; Supplementary Table 20).', '\nFunctional characterization of APOE 4 resiliency-associated proteins.', 'FB, Perivascular Fibroblast; PM, Perivascular Macrophage; TC, T-cell; T-PC, Solute transport-Pericyte; VEN, Venous; VINE, Vessel Isolation and Nuclei Extraction for Sequencing\nWe identified 25 molecules downstream of top candidate proteins ANGPTL4, PTX3, and NCR1 using Ingenuity Pathway Analyses (', ' 6F).', ' 6F).']","Fig. 6 Functional characterization of 4 resiliency-associated proteins. GTEx database gene expression in brain, whole blood, and other tissues using data available from postmortem samples [ ]. The expression of cognate genes coding for proteins associated with non-resilient versus resilient status are displayed in transcripts per million. Genes and tissues are grouped on the y- and x-axis based using hierarchical clustering. . Brain and vascular cell-specific expression of genes coding for proteins associated with non-resilient versus resilient status [ ]. Values are expressed in terms of average normalized counts. . - . Brain and vascular cell-specific expression of genes coding for proteins associated with non-resilient versus resilient status. Expression values are presented stratified by Alzheimers disease verses control status [ ]. Using Ingenuity Pathway Analysis (IPA), we identified molecules downstream of top candidate proteins associated with resilient versus non-resilient status among e4 carriers. Relationships between molecules (edges) were defined based on activation, causation, chemical-chemical interactions, chemical-protein interactions, inhibition, modification, molecular cleavage, phosphorylation, protein-DNA interactions, protein-protein interactions, protein-RNA interactions, regulation of binding, RNA-RNA interactions, transcription, translocation, and ubiquitination. We identified the top three canonical pathways (based on number of overlapping molecules) among the candidate proteins and downstream molecules (left) and included three canonical pathways implicated in Alzheimers disease and related dementia (right). Bolded molecules are downstream of one or more candidate protein and implicated in AD. : aaSMC, Arteriolar Smooth Muscle Cell; ART, Arterial; aSMC, Vascular Smooth Muscle Cell; AST-Ctx, Astrocyte-Cortex; AST-Hpc, Astrocyte-hippocampus; CAP, Capillary; EPEN, Ependymal; M.FB, Meningeal Fibroblast; MCR, Motoric Cognitive Risk; MG, Microglia; M-PC, ECM-regulating Pericyte; NEU, Neuron; OL, Oligodendrocyte; OPC, Oligodendrocyte Precursor Cell; P.FB, Perivascular Fibroblast; PM, Perivascular Macrophage; TC, T-cell; T-PC, Solute transport-Pericyte; VEN, Venous; VINE, Vessel Isolation and Nuclei Extraction for Sequencing",yes
PMC4488692,Figure_2,oa_package/85/eb/PMC4488692.tar.gz,"['The fever (B) and heart rate (C) curves, shown here as individual animals, were averaged and shown as the controls in of reference [21].', 'Table 2 and show changes observed in CBC; Table 3 and show clinical chemistries.', 'Hematologic changes in individual macaques.', 'Abnormalities were observed in both the WBC and RBC compartments (Table 2, ).']",Figure 2 Hematologic changes in individual macaques. White cell counts ( ) and red cell indices ( ) are compared prior to and 2022 h following ricin exposure.,yes
PMC10610814,Figure_1,oa_package/80/23/PMC10610814.tar.gz,"['Design and Construction of the OMV-Based SARS-CoV-2 VaccineAs shown by the 3D structure of the SARS-CoV-2 RBD in complex with ACE2, the RBM of the spike protein is organized in two regions spanning 5 and 6 strands, which include a majority of the spike residues contacting ACE2 (A).', 'To this end, the nucleotide sequence encoding the strands 5 (RBM438 462) and 6 (RBM467 509) or a combination of the two (RBM438 509) were fused to the 3 end of the sequence encoding FhuD2 in plasmid pET-FhuD2 [9] (B).', 'SDS-PAGE analysis revealed protein species with molecular weights corresponding to the designed fusion proteins (C).', '1007/s00281-023-00996-237436465Construction and production of OMVs carrying SARS-CoV-2 RBM antigens.']","Figure 1 Construction and production of OMVs carrying SARS-CoV-2 RBM antigens. ( ) Topology of the interaction between SARS-CoV-2 RBD and ACE2 with the indication of the two RBM polypeptides tested in this study. ( ) Schematic representation of the pET21-based constructs expressed in BL21(DE3) to decorate OMVs. Ferric hydroxamate receptor 2 (FhuD2) was fused to one copy of RBM , RBM and RBM SARS-CoV-2 epitopes. ( ) SDS-PAGE of purified OMVs derived from BL21(DE3) cultures expressing pET21-FhuD2-RBM , pET21-FhuD2-RBM and pET21-FhuD2-RBM plasmids or the control pET21-FhuD2 or empty pET21 plasmids. Stars indicate the predicted migration of the fusion proteins.",yes
PMC6269335,Figure_30,oa_package/0c/9a/PMC6269335.tar.gz,[],Fig. 30 An algorithm of the management of fibrous dysplasia,yes
PMC11548667,Figure_3,oa_package/36/5d/PMC11548667.tar.gz,"['The images were then subjected to further augmentation including rotation, contrast adjustments, and brightness modifications to enrich data diversity ().', '(a d) are the images obtained as a result of image enhancement.']",Figure 3 ( ) are the images obtained as a result of image enhancement.,yes
PMC8320059,Figure_3,oa_package/a3/da/PMC8320059.tar.gz,"[' 3, 4 and 5).', 'Ethmoid sinus volume anterior limit.', 'On the corresponding coronal view (right image), region of interest (ROI) tool is used to mark out the ethmoid sinus volumeEthmoid sinus volume posterior limit.']","Fig. 3 Ethmoid sinus volume anterior limit.On the axial view (left image), anterior limit is set at level of posterior lacrimal crest. On the corresponding coronal view (right image), region of interest (ROI) tool is used to mark out the ethmoid sinus volume",yes
PMC6606718,Figure_4,oa_package/bc/c5/PMC6606718.tar.gz,"['M Activation Profile in Vaccinated Mice ( Tc)The splenic and LN profile of M are presented in and S2.', 'non-vaccinated controls, A).', '01, A), while booster immunization with fTr or fTr+QA (gp5 and gp6) resulted in increased expression of CD205 only (vs.', 'normal controls, A).', 'Antigen presenting capacity and functional profile of splenic macrophages (M ) in vaccinated mice ( T.', 'Except for an increase in CD206hiCD11b+ M population (C), inclusion of fTr booster had non-significant effects on TcG2/TcG4-induced M activation before or after challenge infection, and co-delivery of QA had an overall suppressive effect on the splenic CD11b+ M in vaccinated mice (s 4C F).', 'Non-vaccinated mice exhibited an increase in CD206lo M sub-population post-challenge infection (F).', 'Together, the results presented in and S2 suggest that (a) TcG2/TcG4 DNA vaccine induced M depot was capable of responding to T.', '7 22%) indicative of T cell recruitment and early activation (E).']","Figure 4 Antigen presenting capacity and functional profile of splenic macrophages (M) in vaccinated mice ( ). Mice were vaccinated and challenged with . trypomastigotes as in , . Splenocytes were labeled with fluorochrome-conjugated antibodies and analyzed by flow cytometry. Bar graphs show percentages of the splenic Ly6G CD11b M that exhibited surface expression of markers of maturation, antigen uptake, and antigen presentation (CD205 , CD209 , MHCI , MHCII , CD80 ) in vaccinated and vaccinated/infected mice. Splenic cells from vaccinated and vaccinated/infected mice were stimulated for 48 h in the presence of soluble lysate (TcL), and then labeled with fluorochrome-conjugated antibodies. Shown are the mean percentages of CD11b M that responded to TcL stimulation with expression of markers of classical/proinflammatory (IL-1 , TNF- ) and alternative/immunomodulatory (CD200 , CD206 ) phenotype. Splenic cells from non-vaccinated/non-infected and non-vaccinated/infected mice were used as controls. Data (mean SD) are representative of two independent experiments ( = 3 mice per group per experiment, duplicate observations per mouse), and significance is annotated as *none vs. vaccinated or infected vs. vaccinated/infected, and comparison of vaccinated groups (*, < 0.05, **, < 0.01, and ***, < 0.001).",yes
PMC5990000,Figure_3,oa_package/80/2f/PMC5990000.tar.gz,"['The M portal is used to obtain direct and best access for the extraction of the loose bodies that are located in the subcoracoid bursa (A).', '(A) The patient is in beach-chair position, right shoulder.', 'With a spinal pincer grasp (Intervertebral Disc Pincer; Stryker), loose bodies are removed gradually from the medial portal (B, Video 1).']","Fig 3 (A) The patient is in beach-chair position, right shoulder. Through the I portal, the coracoid process (black arrow), conjoint tendon (white arrow), and pectoralis minor are visualized. (B) With good visualization from the I portal (performed in front of the tip of the coracoid process), a disc pincer is introduced through the M portal (transpectoral medial portal) to extract the loose bodies located in the subcoracoid bursa, directing the pincer toward the lateral side to avoid injuring the plexus (asterisk). The black arrow indicates the coracoid process; white arrow, conjoint tendon.",yes
PMC2653195,Figure_2,oa_package/b1/76/PMC2653195.tar.gz,"['001)(a i, k m, Table 1).', 'The fiber cross-sectional area (CSA) distribution of WT and 10 30 chimeric diaphragms co-peaked at 500 m2 (mode) and the values were higher than those of the mdx or 5 chimeric diaphragms (300 m2 and 400 m2 respectively), probably due to the presence of small caliber, regenerating ( 300 m2) fibers in the mdx or 5 muscles (j and Table 1).', 'Interestingly, all the chimeric and the mdx diaphragms exhibited a high percentage of fibers with CSAs greater than 900 m2 (26 for 10 30 chimeras, 56 5 chimeras and 17 for mdx compared to 6 for WT)(j), suggesting the presence of hypertrophic fibers in the chimeric muscles (P 0.', 'g002WT/mdx chimeric muscle exhibits improved pathology in a dose-dependent manner.']",10.1371/journal.pone.0004759.g002,yes
PMC9705147,Figure_3,oa_package/e4/3f/PMC9705147.tar.gz,"[""Malignant lesions involving the hip include primary bone tumors such as osteosarcoma, bone metastases, and plasmacytoma, depending on the patient's clinical history (\n\n)."", '\nBone metastasis in a 40-year-old male patient presenting with hip pain.']","Fig. 3 Bone metastasis in a 40-year-old male patient presenting with hip pain. Sagittal proton density fat-suppressed (PD FS) ( ), axial T1 ( ), and axial PD FS magnetic resonance arthrogram images ( ) show an irregular aggressive acetabular lesion ( ) with an associated soft tissue component ( ).",yes
PMC7849772,Figure_2,oa_package/f0/57/PMC7849772.tar.gz,"['Inhibited miR-494-5p or elevated TIMP3 alleviates pathology and decelerates cell apoptosis in nucleus pulposus tissues of IDD miceResults of RT-qPCR and Western blot analysis (a) suggested that miR-494-5p expression was increased, and TIMP3 expression was decreased in mice s nucleus pulposus tissues of the IDD model.', '.', 'Data are expressed as mean standard deviation and one-way ANOVA was used for comparisons among multiple groupsThe HE staining showed that ((d)) there were more notochord cells or chondrocyte-like cells, and the cells evenly arranged in the normal mice; in the IDD modeled mice, the nucleus pulposus cells were sharply decreased and disorderedly arranged.', 'The outcomes of TUNEL staining (e) mirrored that IDD modeled mice had more TUNEL positive cells in nucleus pulposus tissues than normal ones; inhibition of miR-494-5p or overexpression of TIMP3 decreased the TUNEL positive cells in nucleus pulposus tissues of IDD mice; effect of suppressed miR-494-5p on TUNEL positive cells was reversed by inhibition of TIMP3.']","Figure 2. Inhibited miR-494-5p or elevated TIMP3 alleviates pathology and decelerates cell apoptosis in nucleus pulposus tissues of IDD mice. (a), miR-494-5p expression in mices nucleus pulposus tissues of each group; (b), TIMP3 mRNA expression in mice nucleus pulposus tissues of each group; (c), protein expression of TIMP3 in mice nucleus pulposus tissues of each group; (d), representative images of HE staining in nucleus pulposus tissues of mice in each group; (e), representative images of TUNEL staining in nucleus pulposus tissues of mice in each group; (f), statistical results of TUNEL staining in nucleus pulposus tissues of mice in each group; n=8, a <0.05 the normal group, b <0.05 the NC group, c <0.05 the anti-miR-494-5p group. Data are expressed as mean standard deviation and one-way ANOVA was used for comparisons among multiple groups",yes
PMC8745457,Figure_5,oa_package/3e/11/PMC8745457.tar.gz,"['When the values describing density modification before and long after the procedure were compared, they showed a statistically significant difference, which supports the thesis that a successful treatment can destroy the pathological tissue and induce a restitutio ad integrum of the affected bone segment (, and ).', 'An OO of the distal diaphysis in an 8-years old girl treated successfully with MRgFUS.']",Figure 5 An OO of the distal diaphysis in an 8-years old girl treated successfully with MRgFUS. A complete restitution ad integrum was observed (follow-up time: 24 months).,yes
PMC10749125,Figure_2,oa_package/46/b9/PMC10749125.tar.gz,"['The KI-67 index varied but was up to 10% (figure 2).', 'Immunohistochemical staining revealed expression of (A) ISLET-1 and dot-like positive stain for pan cytokeratin (AE1/3) ( 20) and (B) synaptophysin ( 2) in the peripheral part of the tumour, (C) CD56 in the centre of the tumour and (D) SOX-10 ( 2) and (E) S-100 ( 2) in the peripheral part as well as the centre of the tumour.', 'The stains for neuroendocrine cells, CD56 and Synaptophysin (figure 2B,C) were performed twice due to lack of staining central in the tumour; however, these did not differ from the first stain.']","Figure 2 Immunohistochemical staining revealed expression of (A) ISLET-1 and dot-like positive stain for pan cytokeratin (AE1/3) (20) and (B) synaptophysin (2) in the peripheral part of the tumour, (C) CD56 in the centre of the tumour and (D) SOX-10 (2) and (E) S-100 (2) in the peripheral part as well as the centre of the tumour. The Ki-67 index (20) varied between the border (F) and the centre (G) of the tumour but was up to 10%.",yes
PMC10381547,Figure_1,oa_package/ff/c6/PMC10381547.tar.gz,"['The most common clinical forms are diffuse scleritis and nodular scleritis [3] ().', '1186/s13643-020-01314-932164765Anterior segment picture of diffuse scleritis of the temporal part of the left eye.']",Figure 1 Anterior segment picture of diffuse scleritis of the temporal part of the left eye.,yes
PMC8712948,Figure_2,oa_package/68/75/PMC8712948.tar.gz,[],"Figure2 Strategy for the generation of an inducible Ndufa4l2 knockdown ES cell line. Several shRNA sequences were cloned into the L3GGEPPIR vector, and evaluated in gene-silencing assays using a luciferase reporter system. The most potent hairpin (shNdufa4l2.304) was cloned into the cTGME vector for genomic targeting into the modified Col1a1 locus of the KH2 ES cell line. Ndufa4l2 gene silencing assessed in polyclonal cell lines with specific shRNA expression. The knockdown potency of individual shRNAs was assessed by transient introduction of an Ndufa4l2-tagged psiCHECK2 dual luciferase reporter. In brief, a dox-inducible shRNA (top left) targets the Ndufa4l2 region of the LucR-Ndufa4l2 fusion transcript, thereby decreasing the expression of LucR protein. The LucR activity was normalized to the activity of LucF expressed from a constitutive active promoter (HSV-TK). Dox-inducible shRNA expression was confirmed by visualization of EGFP in more than 95% of the stably transfected cells. Generation of a stable cell line and the mating scheme for breeding the triple-transgenic TANdu mouse line. ES cells were nucleofected with plasmid constructs cTGME (targeting vector) and pCAGs-Flpe (expression vector) for the targeted integration into the recipient FRT-site in the locus. Correct integration introduces expression of the HygR selection marker, and is confirmed using Col1A1(+), Col1A1 (), and SAdpA () primers (red, green, and blue triangles, respectively). Validated ES cells were injected into blastocysts to generate shNdufa4l2 transgenic animals. Three validated shNdufa4l2 males were mated with females carrying the transgenic HIF1 (TRACK) and a ggt-driven tet-Transactivator (ggt tTA). Ndufa4l2 protein levels are decreased in the shNdufa4l2 mice (shNdu) relative to the TRACK ccRCC model (to 57 7%). Kidney cortex protein was isolated from kidneys of three male TANdu mice (6 months old, ID#19-259). In addition, protein was isolated from age-matched male WT and TRACK mice (one and three mice, respectively). The antibodies used are specified in the Method section.",yes
PMC2726921,Figure_4,oa_package/a2/bc/PMC2726921.tar.gz,"['01) in animals null for Irs2 compared to wild-type animals (A,C).', '05) (B).', 'Increase in insulin degrading enzyme and in transthyretin in mice lacking Irs2.']",Fig. 4 Increase in insulin degrading enzyme and in transthyretin in mice lacking . Amyloid turnover is regulated by proteolysis through proteases such as insulin degrading enzyme (IDE) and by increased clearance following binding to proteins such as transthyretin; both known to be altered in response to insulin signalling. IDE mRNA was unaltered in IRS2/ mice (A) but protein levels in the membrane-bound fraction were modestly increased (B). Transthyretin mRNA was increased substantially (3.9-fold; <0.02; SEM 1.05) in both genotypes lacking .,yes
PMC6134924,Figure_2,oa_package/fd/11/PMC6134924.tar.gz,"[' In CMV+ people, CD28null CD8 T-cell numbers are about twice as high as in CMV- people.']","Figure 2 Dotplots show CD27 versus CD28 expression on CD8 T-cells in CMV- and CMV+ individuals. CD28 cells are indicated (circle). The CD28 CD8 T-cell frequency distribution (log10-transformed CD8 T-cell fractions) is shown in the whole cohort (top, n=242), CMV- (middle, n=106) and CMV+ individuals (bottom, n=136). Scatterplots show log10-transformed fractions of CD28 CD8 T-cells. Scatterplots show log10-transformed fractions of the CD27+ and CD27- subsets of CD28 CD8 T-cells. Error bars in scatterplots show median, upper, and lower quartiles. The ROC curve shows the separation of CMV- and CMV+ populations by CD28 CD8 T-cell frequencies.",yes
PMC9552802,Figure_1,oa_package/9f/fb/PMC9552802.tar.gz,"['ResultsSpecificity of anti pThr509-CRMP1 antibodyTo specifically investigate the phosphorylation status of CRMP1 in ALS spinal cords, we used anti pThr509-CRMP1 antibody raised against mouse/rat CRMP1 (503 515) peptide (A) instead of the commonly used phospho-antibody against Ser522-CRMP1/2, which is an identical phosphorylation consensus motif for CRMP1 and CRMP2.', 'The reactivity of our antibody with human CRMP1 was verified by western blotting using HEK 293T cell lysate transfected with human CRMP1, either with or without exogenous Cdk5 expression (B).', 'Using the brain tissues of Crmp1 knockout mice, the specificity of this antibody for CRMP1 was further confirmed by negative results with immunoblotting of brain lysate (C).', 'pThr509-CRMP1 immunoreactivity was observed in areas corresponding to the dendrite-rich molecular layer of the hippocampal dentate gyrus in WT mice, but not in CRMP-1KO mice (D).', 'Specificity of anti pThr509-CRMP1 antibody.', 'In this study, an anti pThr509-CRMP1 antibody was raised against the mouse/rat CRMP1 peptide (A), and Thr509 in CRMP1 is reported to be directly phosphorylated by Cdk5 in rodents (33).', 'As shown in B, we demonstrated that anti pThr509-CRMP1 antibody successfully detected human pCRMP1, and Cdk5 phosphorylated CRMP1 in human cells (HEK 293T) without the need for GSK3 .']","Figure 1 Specificity of antipThr509-CRMP1 antibody. Sequence comparison of the C-terminal domain in human and mouse CRMP1. Underlined letters indicate the antigen peptides comprising the antipThr509-CRMP1 antibody. Phosphorylation of Thr509 in CRMP1 by Cdk5. HEK 293T cells were transfected with human CRMP1 and Cdk5. The antipThr509-CRMP1 antibody successfully recognized pThr509 in human CRMP1. Immunoblotting of brain lysates of WT and Crmp1 knockout KO mice with antipThr509-CRMP1 antibody. pThr509-CRMP1 signals are lost in Crmp1 KO mice. DAB labeling of hippocampi of WT and Crmp1 KO mice with an antipThr509-CRMP1 antibody. The lower panels show enlargement of the area enclosed by the dotted line in the upper panels. Brown DAB chromogen is observed in the dendrite-rich molecular layer of the hippocampal dentate gyrus in WT mice, but is not seen in Crmp1 KO mice. Scale bars = 100 m (top); 20 m (bottom). DG, dentate gyrus; G, granule cell layer; M, molecular layer.",yes
PMC11276268,Figure_4,oa_package/29/4a/PMC11276268.tar.gz,"['In this research, the Canny edge detection [17] is used to first extract the edges from the images, which potentially contain the marker edges (a).', 'It is possible that not all the targets were detected because no edge points were found (for example, two markers, as shown in a.', 'b shows the markers extracted from the image with increased intensity.', 'Target-extracting illustration; (a) edge detected from the image; (b) complete targets extracted.']","Figure 4 Target-extracting illustration; ( ) edge detected from the image; ( ) complete targets extracted. Yellow lines: detected edges, red lines: target ellipses, and red dots: target centers.",yes
PMC7086115,Figure_2,oa_package/2f/de/PMC7086115.tar.gz,[],Figure 2 Prepyloric deformity (red arrow),yes
PMC9549961,Figure_1,oa_package/f8/e8/PMC9549961.tar.gz,"['5 cm in diameter was observed, containing a central orifice (A).', 'Pet cat treated at the dermatology service of the Veterinary Hospital of Federal Rural University of Rio de Janeiro naturally infested by Dermatobia hominis.', 'hominis from the tail, and a second larva, still alive, from the base of the tail (B).', 'The use of topical isoxazolin was recommended, along with sanitizing lesions with 1% chlorhexidine until total healing (C and 1D).']","Figure 1 Pet cat treated at the dermatology service of the Veterinary Hospital of Federal Rural University of Rio de Janeiro naturally infested by . (A) Purulent secretion at the base of the tail; (B) Maggot collected from the cat by digital compression, where the larger larva with darker coloration was dead at the time of removal; (C) Ulcerated nodular lesion in the tail; (D) Ulcerated nodular lesion located at the base of the tail. Source: Personal archive.",yes
PMC2566566,Figure_2,oa_package/f4/fb/PMC2566566.tar.gz,['Abdominal Plain Film.'],Figure 2 Black arrow showed a large mass (the fecal calculus) with clear border on the left upper quarter of the abdomen. White arrow head show two vapor-liquid levels.,yes
PMC5697377,Figure_5,oa_package/5b/3a/PMC5697377.tar.gz,"['This loss was significantly inhibited by OSS treatment at 50, 100, and 200 mg/kg/day in the CA3 region of the mouse hippocampus ().', '004""/>\nEffect of OSS on hippocampal cell death induced by A O\n1 42\n toxicity.']","Figure 5 Mice were treated with vehicle or OSS for 14 days, starting from 5 days before stereotaxic injection of A O . Hippocampal cell loss was determined using cresyl violet staining. Quantification was performed by measuring the cell intensity of stained cells in the GCL (a) and in the CA3 (b). Representative photomicrographs are shown for the GCL (cg) and CA3 (hl) from each group (400x magnification). Scale bar = 50 m. (c, h) Sham-operated group; (d, i) A O only treated group; (e, j) A O + OSS 50mg/kg/day group; (f, k) A O + OSS 100mg/kg/day group; (g, l) A O + OSS 200mg/kg/day group. Data are expressed as percentages relative to sham-operated group. Values are indicated as the mean SEM. < 0.05 and < 0.001 compared to sham-operated group; < 0.001 compared to the A O -only treated group.",yes
PMC5447273,Figure_1,oa_package/77/f0/PMC5447273.tar.gz,['173Rat retinal neurons and glial cells express IL-1 and IL-1RI.'],"Figure 1 Rat retinal neurons and glial cells express IL-1 and IL-1RI. IL-1 (a) and IL-1RI (b) immunostaining (both in green) can be observed in rat retinal neurons (TUJ-1; red) and microglia (CD11b or iba-1; red) and macroglial cells (Mller cells and astrocytes; GFAP; red), indicating that retinal neural cells express IL-1 and can be responsive to it, since they express IL-1RI. Scale bar: 50 m.",yes
PMC10528153,Figure_6,oa_package/12/2d/PMC10528153.tar.gz,"['In LUHS DTI and white matter, tractography is performed for surgery planning when tumoral involvement of major white matter tracts is expected ().', 'DTI image data superimposed on axial T1W postcontrast image for anatomic localization.']",Figure 6 DTI image data superimposed on axial T1W postcontrast image for anatomic localization. Invasion of the left corticospinal tract is seen by a left frontal glioblastoma (arrow). The image used for publication is taken from LUHS Radiology Clinic archives servers.,yes
PMC8595014,Figure_4,oa_package/03/fe/PMC8595014.tar.gz,"['The nodules were further evaluated with a dedicated CT chest, abdomen, and pelvis with intravenous contrast that showed two nodular masses along the anterior surface of the diaphragm, suspicious for a mesothelial process () and thickened, enhancing omentum and ascites ().', '003"" position=""float""/>(a) Axial CT with intravenous contrast of the chest demonstrating pleural-based nodules.']",Figure 4 (a) Axial CT with intravenous contrast of the chest demonstrating pleural-based nodules. (b) Coronal CT with intravenous contrast of the chest demonstrating pleural nodules.,yes
PMC5128518,Figure_3,oa_package/99/bb/PMC5128518.tar.gz,"[""Conventional radiography plain films of the patient's knees revealed Erlenmeyer flask deformity, characterized by marked femorotibial metaphyseal flaring, and associated cortical bone thinning ("", '']","Fig.3 Knee radiographs of patient 1. Conventional knee radiographs in anteroposterior view showing Erlenmeyer flask deformity, characterized by femorotibial metaphyseal flaring, cortical bone thinning, and genu valgum.",yes
PMC7752607,Figure_1,oa_package/64/cd/PMC7752607.tar.gz,[],"FIGURE 1 Before treatment with tofacitinib 5mg/BID; previous treatments included topical corticosteroids, tacrolimus ointment, moisturizers, prednisone, methotrexate, azathioprine, cyclosporine, antihistamines, antidepressants, thalidomide, and phototherapy",yes
PMC8907432,Figure_3,oa_package/88/4f/PMC8907432.tar.gz,[],Image 3 Pelvic lymphocele after drainage,yes
PMC11056332,Figure_3,oa_package/8f/cf/PMC11056332.tar.gz,[' 3A B Transthoracic Apical five-chamber (A5C) view with color Doppler compare on initial presentation in Case-5 showing multiple intracardiac rhabdomyomas in the left ventricle (LV) crowding the intraventricular cavity and left ventricular ouflow tract (LVOT) and a large tumour in the right atrium partially obstructing the right ventricular inflow'],Fig.3 & Transthoracic Apical five-chamber (A5C) view with color Doppler compare on initial presentation in Case-5 showing multiple intracardiac rhabdomyomas in the left ventricle (LV) crowding the intraventricular cavity and left ventricular ouflow tract (LVOT) and a large tumour in the right atrium partially obstructing the right ventricular inflow,yes
PMC11670280,Figure_7,oa_package/19/40/PMC11670280.tar.gz,['Inflammatory markers and ECM proteins were quantified using immunohistochemistry on non inflamed (LPS ) and inflamed (LPS +) tissues.'],"Figure 7 Inflammatory markers and ECM proteins were quantified using immunohistochemistry on noninflamed (LPS ) and inflamed (LPS +) tissues. Fluorescent images were created through a 2D maximum intensity projection of a 70m thick tissue where the mean fluorescence intensity was measured for at least four different ROIs per chip. Each data point represents one individual chip. Data presented as mean SD (ns = not significant, < 0.01, < 0.001, = 10). Scale bars: 100m.",yes
PMC5129924,Figure_6,oa_package/5e/cb/PMC5129924.tar.gz,['Delay in neuronal migration by Prrt2 knockdown during developmentA.'],"Figure 6 Delay in neuronal migration by Prrt2 knockdown during development Coronal sections of the mouse brains 3 and 5 days after electroporation of shControl or shPrrt2 constructs at E14.5. Three days after electroporation, cells expressing EGFP (green) and shControl had started migration, with many cells having already reached the CP. More cells were restricted to the VZ and IZ in the brains electroporated with each of the shPrrt2. This migration delay was rescued by co-transfection of shPrrt2 and Prrt2 constructs (upper panel). When brain sections were examined 5 days post electroporation, most cells in both shControl and shPrrt2 groups had reached the CP (lower panel). All sections were stained with DAPI (blue) to show the cell nuclei. Scale bars = 100 m. Statistical analysis showed significant differences in cell distribution in the CP, IZ and VZ at E17.5for the four conditions. Error bars represent SEM; *: < 0.05, ** : < 0.01.",yes
PMC6156255,Figure_6,oa_package/05/8f/PMC6156255.tar.gz,['Visualization of dendritic cell diversity.'],"Figure 6 Visualization of dendritic cell diversity. To avoid the cross-batch effect, we used a single experiment including 15 samples of virgin uterus, decidua and placenta across the whole murine pregnancy. t-SNE map generated from pre-gated lin1-I-A/I-E+ cells from murine virgin uterus, decidua and placenta across gestational age data sets (top) and manually gated subsets overlaid onto total lin1-I-A/I-E+ cells (bottom). Hierarchical clustering of median surface marker expression levels of clusters identified by DensVM. Total of 16 clusters were categorized in 6 groups, group AF. Pie chart of different group proportions of total DC population. Hierarchical clustering of cluster frequency within lin1-I-A/I-E+ from murine virgin uterus, decidua and placenta across gestational age. Separate visualization of cluster in different murine tissues across gestation using t-SNE map generated from the merged data set.",yes
PMC3050766,Figure_2,oa_package/e8/e9/PMC3050766.tar.gz,['Reduction of hippocampal A in AAV2/1-mTNF expressing TgCRND8 mice.'],"Figure 2 . . 4 month old TgCRND8 mice were stereotaxically injected in the hippocampus with either AAV2/1-mTNF or AAV2/1-EGFP and sacrificed after 6 weeks ( = 5-6/group). Representative brain sections stained with 33.1.1 antibody (pan A 1-16) depict attenuation of A deposition in mTNF expressing mice (C-D) compared to controls (A-B) in the immediate vicinity of the injection site. , 150 m. . A plaque burden image analysis shows a significant decrease in amyloid deposition in mTNF injected mice compared to control EGFP injected mice. At least three sections per sample, spaced 30 m apart, were used for the analysis. (* = 0.033; test; n = 5/group). . Quantitation of Congo Red stained compact A plaques in mTNF injected mice compared to control EGFP injected mice. Hippocampal compact plaques were counted from coronally dissected paraffin embedded representative brain sections of mTNF and EGFP expressing mice. (* = 0.05; test; n = 5/group). . Biochemical analyses of A42 and A40 levels by ELISA show significantly reduced SDS (G) and formic acid (H) extractable A levels in mTNF injected mice compared to controls (* = 0.05; two way ANOVA; n = 4/group).",yes
PMC8320374,Figure_2,oa_package/32/82/PMC8320374.tar.gz,[],"Figure2 ST2 deficiency amplified the susceptibility to DSS-induced colitis. DSS was orally administered to mice and BALB/c WT mice (n = 10-14 mice per group) for 6 days, followed by one day of normal drinking water. Body weight changes relative to the initial weight and disease activity index were monitored daily. Histopathological score (n = 6) and representative Hematoxylin and Eosin (H&E)-staining pictures of healthy control (ctrl) and inflamed colons (DSS) were evaluated on day 7. Scale bars represent 100 m. Secreted levels of IL-33, TNF- and IL-6 were determined in the supernatants of colonic explants Luminex technology. All data are presented as mean SEM. Statistical analyses were performed using two-way ANOVA, followed by Tukeys multiple comparison test , unpaired Students t-test , or one-way ANOVA followed by Dunns or Dunnetts multiple comparison test . * < 0.05; *** < 0.001.",yes
PMC10946294,Figure_3,oa_package/2a/39/PMC10946294.tar.gz,"['CT scan in portal-venous phase for further pre-operative investigation showing the appendicolith in coronal and axial view (the latter in different grey-level mapping), in concave shape and measuring 4.']","Figure 3 CT scan in portal-venous phase for further pre-operative investigation showing the appendicolith in coronal and axial view (the latter in different grey-level mapping), in concave shape and measuring 4.0 cm 1.5 cm 1.9 cm here.",yes
PMC6610396,Figure_1,oa_package/57/51/PMC6610396.tar.gz,"['Computed tomography (CT) performed for evaluation of the laryngeal lesion confirmed\nthe mandibular tori (\n1 Left).', '.', '1177_1179547619856599-fig1""/>In the operating room, attempts to visualize the upper border of the lesion with\nmultiple laryngoscopes were limited.', 'CT exam with 3D reconstruction confirmed the\nbilateral mandibular tori (\n1 Right).']",Figure 1. (Left) 3-D reconstruction of CT Neck showing bilateral mandibular tori fromcase 1 (Right) 3-D reconstruction of CT Neck showing bilateral mandibulartori from case 2.,yes
PMC10716465,Figure_3,oa_package/fd/6f/PMC10716465.tar.gz,[],"Figure3 The long-term vascular responses to bacterial peritonitis are exacerbated in animals routinely exposed to PD solutions. C57BL/6 mice (n=6/group) were fitted with a peritoneal catheter, given a 7-day recovery period and instilled once daily with 2ml PBS or PDF for 14 day. Mice were then i.p. injected with or PBS (Day 0) and culled at Day 1 or further exposed daily to PBS or PDF, prior to culling at Day 28. Peritoneal lavages, blood and aortas (Day 28 only) were collected and proportions of innate immune leukocytes in blood and lavages were determined by flow cytometry and plasma and peritoneal levels of cytokines and endothelial activation markers (plasma only, ) were quantified by ELISA 0.05 0.01 0.005, ordinary one-way ANOVA (normal distribution) or Kruskal-Wallis test (non-normal distribution). Volcano plots compare the effect of , PDF exposure + and PDF exposure to PBS control on atherosclerosis-associated gene expression in aortas at Day 28. Red (upregulated, fold change 2) and green (downregulated, fold change 0.5) circles represent single genes significantly affected ( 0.05, represented by the horizontal line) compared to PBS control.",yes
PMC2803955,Figure_3,oa_package/97/93/PMC2803955.tar.gz,['Inmunohistochemistry presented positivity high molecular weight cytokeratins (A)( 40) and CEA (B) ( 40).'],Figure 3 .,yes
PMC8628155,Figure_6,oa_package/14/f0/PMC8628155.tar.gz,"['8%) () [129].', 'Indeterminate LNs are defined as LNs that do not have imaging features of suspicious or benign LNs (Supplementary ).', 'pdf"" id=""d64e10534"" position=""anchor""/>Supplementary Indeterminate lymph nodes on CT.', 'Ultrasonography feature of extrathyroidal extension of thyroid cancer to the trachea.']",Fig. 6 Ultrasonography feature of extrathyroidal extension of thyroid cancer to the trachea. An obtuse angle (white line) was formed between the surfaces of the trachea and thyroid cancer. Diagnosis: gross extrathyroidal extension to the tracheal wall. Adapted from Chung et al. Korean J Radiol 2020;21:1187-1195 [ ].,yes
PMC9827764,Figure_1,oa_package/27/e1/PMC9827764.tar.gz,"['For processing, tissue samples were transferred to a 90 mm petri dish containing chilled Hibernate-A, and the dimensions of each fragment were measured to estimate tissue volume ().', 'Schematic diagram showing the production of organotypic neural slices, derived from cerebellar tonsillar tissue resected during decompressive surgery for Chiari malformation.', ', 2014), a penetrating, transecting injury was induced in a subset of slices at 8 days post slice derivation with the tissue from within the lesion removed using an aspirator (: inset).']","Figure 1 Schematic diagram showing the production of organotypic neural slices, derived from cerebellar tonsillar tissue resected during decompressive surgery for Chiari malformation. Arrows denote transecting injury within neural slices; inset shows the removal of tissue from lesion site following transecting injury.",yes
PMC6704256,Figure_1,oa_package/be/c8/PMC6704256.tar.gz,"[' 1a, b; as well established16), we also observed non-plaque-associated microglia, including ramified microglia, accumulating aggregates within lysosomal compartments (', ' 1c, d), and microglia containing intracellular aggregates the size of small plaques (', ' 1e, f).', ' 1g j).', 'Plaque-distal microglia contain aggregated A .', 'Scale bars = 40 m for g, i 10 m for h, jDevelopment of a CSF1R inhibitor for microglial eliminationTo explore the roles of microglia in the initial development of plaque pathology, we required a method that allowed for the extended and specific elimination of microglia throughout the plaque forming period, using non-invasive/non-stressful approaches.', ' 1), as well as the human brain (Supplementary 1).']","Fig. 1 Plaque-distal microglia contain aggregated A. 15-month-old 3xTg-AD mice were stained for dense core deposits with Thio-S (in green), and immunolabeled for microglia (IBA1 in red) and macrophage lysosomes (CD68 in blue; , , and ) with zoomed image ( ) of Thio-S material within microglia and within lysosomes, separately. Scale bars=20 m for , 5m for ,10m for . , Three-dimensional reconstruction of microglia (IBA1 in red), the microglial lysosome (CD68 in purple), and fibrillar A (Thio-S in green), demonstrating the localization of A to the microglial lysosome in non-plaque associated microglia. Scale bars=7m. 5xFAD animals stained for dense-core deposits (Thio-S in green) and immunolabeled for microglia (IBA1 in red; and ), with zoomed images ( , ) demonstrating Thio-S aggregates in microglial cell bodies in 4- and 7-month-old 5xFAD mice. Scale bars=40m for , 10m for ,",yes
PMC6939074,Figure_4,oa_package/10/5c/PMC6939074.tar.gz,"['Horizontal histological sections of the debulking (hematoxylin-eosin, 20).']","Figure 4 Horizontal histological sections of the debulking (hematoxylin-eosin, 20). Top left image corresponding to the surface incision and lower right image to the deepest incision. Highlighted lateral margin involvement (red rectangle).",yes
PMC10373723,Figure_3,oa_package/c9/e1/PMC10373723.tar.gz,"['In the two situations depicted in figure 3A, B the model accurately identified the cancerous region in both instances.', 'In figure 3A, the lung tissue beneath the cancerous spot is more constricted and the staining is deeper than in the surrounding normal lung tissue.', 'Model prediction examples.', 'In figure 3C, there are six components, indicating an adenocarcinoma case with complicated components.']",Figure 3 Model prediction examples.,yes
PMC10533866,Figure_3,oa_package/76/8b/PMC10533866.tar.gz,"['Oftentimes, if a ramp lesion is present, there will be a clear meniscocapsular disruption with probing of the undersurface of the posterior horn (A).', 'Arthroscopic views of a probe reflecting the medial meniscal posterior horn revealing (A) meniscotibial and (B) meniscocapsular disruptions (yellow arrows) indicative of a medial meniscal ramp lesion.', 'Once in the posterior aspect of the knee, the camera is turned towards the posterior medial meniscus to evaluate for a ramp lesion (B).']",Fig 3 Arthroscopic views of a probe reflecting the medial meniscal posterior horn revealing (A) meniscotibial and (B) meniscocapsular disruptions (yellow arrows) indicative of a medial meniscal ramp lesion. View B is obtained via the Gillquist maneuver to visualize posterior to the medial femoral condyle (left knee).,yes
PMC10630515,Figure_3,oa_package/5b/49/PMC10630515.tar.gz,"[' 3).', 'Reasons for using urine for pediatric brain tumors diagnostic.', 'Our laboratory has had a longstanding interest in the development of non-invasive biomarkers designed to aid in the diagnosis, prognosis and therapy of tumors and cerebrovascular disease, including biomarker fingerprints that can distinguish between central nervous system tumors, moyamoya disease and arteriovenous malformations, including the first report of successfully applying this novel methodology specifically to brain tumors in a multicenter trial1 3,5,6,13 19.']",Figure 3 Reasons for using urine for pediatric brain tumors diagnostic.,yes
PMC6968900,Figure_2,oa_package/4e/23/PMC6968900.tar.gz,['Sigmoid isolation for urinary conduit.'],Figure 2 Sigmoid isolation for urinary conduit.,yes
PMC11512103,Figure_8,oa_package/c3/44/PMC11512103.tar.gz,['.'],"Fig. 8. (A) Representative TEM images of longitudinal gastrocnemius muscle section illustrating myofibril organisation. Note a well-organized sarcomeric structure in the gastrocnemius muscle of 10-month-old WT animals, with the parallel alignment of the multiple myofibrils with distinguishing features being labeled. The A-band (dark band) consists mainly of thick myosin filaments, whereas the I-band (light band) is composed of thin actin filaments. Each sarcomere is flanked by a Z-disc region. Conversely, a disorganised sarcomeric structure is observed in the gastrocnemius muscle of 10-month-old LKO animals. (B) GA muscle mass normalised to body weight in 10-month-old LKO animals. Each bar represents the means.e.m. ( =8 WT and =16 LKO; unpaired two-tailed Student's tests; ** <0.01). (C,D) Animals were subjected to inverted screen and pole tests to evaluate muscle strength and motor coordination. (C) In the inverted screen test, there was a significant reduction in latency to fall in 10-month-old LKO animals compared to age-matched WT control animals. (D) Similarly, 10-month-old LKO animals took more time than WT animals to climb down to their home cage from the top of the pole, measured as latency to climb down after T turn. Each bar represents the means.e.m. [ =9-11 animals for each genotype (WT and LKO); unpaired two-tailed Student's tests; * <0.05, *** <0.001]. Scale bars: 2m.",yes
PMC10206380,Figure_4,oa_package/1d/90/PMC10206380.tar.gz,"['Case 3A 3-month-old male infant from a rural area, the youngest of 3 children born to an elderly mother, with no history of congenital malformations.']","Fig. 4 (A-E) An MRI of the entire spine using T1-T2 sequences reveals a failure of lumbar segmentation or the presence of hemivertebrae (A) and partial sacral agenesis (B and C) consistent with Pang type IV. This malformation is indicated by the low-lying conus and sign of syringomyelia (B-D). In this case, the retained medullary cord with the conus located below the lumbar was found to be attached to fatty structure (D and E).",yes
PMC3306944,Figure_2,oa_package/a0/a6/PMC3306944.tar.gz,"['Thus, a possible role of dysfunction glial cells in AD pathogenesis should be considered, especially in the early stages of the disease process ().', '001""/>A diagram depicting a potential role of glia in mediating the synaptic toxicity of A .']","Figure 2 A diagram depicting a potential role of glia in mediating the synaptic toxicity of A . A oligomers presumably secreted from the presynaptic neuron could bind to its putative receptor on the postsynaptic cell, and this could then initiate a signaling cascade leading to activation kinases such as MARK, which then acts on tau, PSD-95, and possibly other synaptic substrates to affect AMPAR removal from the synaptic surface, leading to synapse and spine loss. Alternatively, A could act on glial cells near neuronal synapses, which then release factors such as cytokines to activate signaling molecules such as MARK and cause synapse and spine loss. These two mechanisms are not mutually exclusive and could in fact occur simultaneously to mediate A toxicity.",yes
PMC7035234,Figure_1,oa_package/7e/30/PMC7035234.tar.gz,"[' 1.', 'Findings of case 1.', '2 mV/division, sweep speed 10 ms/division)Two years later he developed postural instability, a cerebellar gait, dysphagia and REM sleep behaviour disorder (RBD).', ' 1).']","Fig. 1 Findings of case 1. Pathological findings. Coronal section of the left cerebral hemisphere showing atrophy and dark discolouration in the putamen (arrows) ( ). Moderate loss of dopaminergic neurons in the substantia nigra ( ). Moderate depletion of neurons with rarefaction of the transvers fibres in the pons (black asterisk) ( ). Moderate loss of Purkinje cells along with loss of myelinated fibres (white asterisk) ( ). Glial cytoplasmic inclusions immunopositive for -synuclein (arrowheads) ( ). Haematoxylineosin staining, -synuclein immunohistochemistry. Bars=50m ( , ); 100m ( , ). Urodynamics findings showing normal compliance, normal bladder capacity, stable detrusor during fill, normal bladder sensation during fill, and acontractile detrusor during the voiding phase. intra-abdominal pressure, intravesical pressure, detrusor pressure, infused volume, urine flow. Concentric needle EMG of the external anal sphincter. Duration of the recorded motor unit is 38.54ms, which is prolonged and suggests chronic reinnervation. The mean duration of MUPs during the study was 19ms (normal<10ms) and the EMG was compatible with a diagnosis of multiple system atrophy (gain 0.2mV/division, sweep speed 10ms/division)",yes
PMC9620665,Figure_1,oa_package/34/8a/PMC9620665.tar.gz,"[' 1a c) using a SegNet model architecture, detailed in Signaevsky et al.', ' 1a) using Aperio ImageScope software.', 'Detection of neurofibrillary tangles (NFT) in phospho-tau (AT8) immunohistochemically stained whole slide images (WSI).', 'Each pixel value corresponds to the probability that it represents an NFTMean clustering coefficient calculationTo estimate the degree of NFT clustering for a given WSI, we represented the spatial distribution of NFTs as a network and calculated the mean clustering coefficient.']",Fig. 1 Detection of neurofibrillary tangles (NFT) in phospho-tau (AT8) immunohistochemically stained whole slide images (WSI). Example of a hippocampal WSI immunohistochemically stained for phosphorylated-tau (AT8). The hippocampus proper (blue) and entorhinal region (red) were manually segmented. High-power (20x) representative image of the hippocampal CA2 subfield showing p-tau positive neurofibrillary tangles. Corresponding output of above image passed through semantic segmentation model that identifies NFT. Each pixel value corresponds to the probability that it represents an NFT,yes
PMC7653145,Figure_8,oa_package/0c/f4/PMC7653145.tar.gz,"['Our team has also conducted a study to identify a lymph node metastasis of lung cancer using a deep learning platform, where false positive segmentation was successfully excluded through the combination of two deep learning platforms, explained in (36).', 'Detection of lymph-node metastasis using a two-step deep learning approach.']","Figure 8 Detection of lymph-node metastasis using a two-step deep learning approach. From original H&E images (A, B), the initial detection of the deep learning algorithm shows both the tumor (arrowhead) and false positive areas (arrow) (C, D). The final detection with application of a two-step approach can eliminate this error by disregarding the germinal center while remaining a true tumor (arrowhead) detection (E, F). Red: tumor identification, blue: others. Magnification: 10.",yes
PMC6238735,Figure_1,oa_package/68/55/PMC6238735.tar.gz,"['Cultured cells were transfected (Supplemental ) with a miR-21 mimic or inhibitor (locked nucleic acid 21 [LNA-21]) and respective controls (n = 4 per group).', 'qPCR analysis of miR-21 levels confirmed a significant and specific effect of the transfections (A and Supplemental ).', 'No significant differences were observed for decorin and laminin 1 (B and Supplemental ).', 'Importantly, secretome levels for the 20 proteins with the highest number of identified spectra, which includes periostin, did not significantly differ after miR-21 mimic or inhibitor transfection (C).', 'First, platelet counts were determined by flow cytometry using allophycocyanin-CD41 staining (Supplemental 1).', 'Two independent lines with 95% megakaryocyte purity (Supplemental 3A) were transfected with a nontargeting control or a miR-21 inhibitor, resulting in a significant decrease of miR-21 levels (E).', 'No significant effect was seen on megakaryocyte purity or maturity (Supplemental 3, B D).', 'Similarly, platelet levels of WASp and TGF- 1 were also unaffected (Supplemental 4).', '03626254008Version 111/02/2018Electronic publicationTransfections of cardiac fibroblasts with miR-21 mimic and inhibitor.']","Figure 1 Transfections of cardiac fibroblasts with miR-21 mimic and inhibitor. ( ) Cardiac fibroblasts (CFs) were isolated from wild-type mice and transfected with miR-21 mimic or LNA-21 (inhibitor), followed by stimulation with TGF-1 or control treatment. Overexpression and inhibition were confirmed by qPCR ( = 8 for each transfection condition; Wilcoxon matched-pairs signed-rank test; lines and error bars represent median [IQR]; note that in 3 samples miR-21 was undetectable after transfection with LNA-21). ( ) Immunoblotting for several extracellular matrix (ECM) proteins showed effects of TGF-1 treatment but not of miR-21 mimic or inhibitor transfection ( = 4 for each condition). Ponceau S staining was used as loading control. C, control mimic/LNA; 21, miR-21 mimic/LNA-21; M , relative mass. TGF-1 +/ indicates treatment 48 hours prior to conditioned media collection. ( ) Proteomic analysis of the CF secretome after transfections with miR-21 mimic or inhibitor identified no significant changes in the 20 most abundant ECM proteins. Four biological replicates were analyzed for each transfection type in the presence or absence of TGF-1 treatment. No statistically significant difference was seen between miR-21 mimic or inhibitor and its respective control for any of the shown proteins, using a FDR < 0.05, calculated with the Empirical Bayes method.",yes
PMC4600255,Figure_3,oa_package/b3/82/PMC4600255.tar.gz,"[' 3(a)), and whilst there were only occasional FUS-positive NCIs and GCIs present in the motor cortex (confirmed by 2 FUS antibodies) the p62 revealed much more extensive immunopositivity.', ' 3(b)).', 'Scale Bar (a)-300 m, a-inset 100 m (b)-50 m, (c)-150 m, (c) inset-80 m, (d) 200 m, (e)-50 m, (f)-200 m, (f) inset-80 m, (g)-30 mv) Unusual p62 staining pattern: Case 6 showed p62 positivity in the hippocampus (but not the dentate gyrus), mainly in the form of neurites with only small numbers of NCIs (', ' 3(c)), there was also mild p62 positivity in the amygdala and basal ganglia (', ' 3(d)-(e)).', ' 3(f) and (g)).']","Fig. 3 Case 6 (p.P525L and p.Y374X mutation) ( ) and illustrates marked microglial activity in the mid-deeper laminae (between ) of the motor cortex. Anti-CD68. Showing the unusual neuronal staining for p62 in the motor cortex including processes (arrows). - revealing the sparse but unusual neuronal p62 immunopositivity in the hippocampal region ( ) and ( ) inset including occasional NCIs ( ), and ( ) the putamen revealing occasional NCIs ( ). Reveals one of the few p62 immunopositive neurons in the putamen, this is also apparently labelling the corresponding axon/dendrites ( ) and appears different in pattern to the occasional FUS positive NCIs. P62 immunopositivity in the frontal neocortex ( ) revealing dot-like positivity and neuronal inclusions ( and ). p62 immunopositive neuron in the temporal neocortex. These inclusions are negative for FUS and TDP-43 and appear to label the nuclear/ perinuclear region and processes ( ) the latter indicating likely axonal/dendritic positivity. Anti-p62. ( )-300m, - 100m ( )-50m, ( )-150m, ( ) -80m, ( ) 200m, ( )-50m, ( )-200m, ( ) -80m, (g)-30m",yes
PMC2587915,Figure_4,oa_package/f9/b9/PMC2587915.tar.gz,"['To assess lung damage and pulmonary inflammation throughout the course of virus infection in WT and MyD88 / mice, we evaluated hematoxylin and eosin stained lung tissue sections from 2, 4 and 6 dpi ().', 'In contrast, WT mice at 2 dpi exhibited pronounced lung inflammation characterized by perivascular cuffing, endothelial and epithelial atypia, and peribronchivascular immune cell infiltration, without the severe denuding bronchiolitis seen in MyD88 / mice ().', 'At 4 dpi, MyD88 / mice continued to exhibit a denuding bronchiolitis, epithelial/endothelial atypia, and the added phenotype of PBV edema without immune cell infiltration around the airway though perivascular infiltration of immune cells was observed ().', 'g004Lung pathology in rMA15 infected WT and MyD88 / mice.']",10.1371/journal.ppat.1000240.g004,yes
PMC8692180,Figure_4,oa_package/ed/ee/PMC8692180.tar.gz,[],FIGURE 4 Abdominal radiographs of a cat with a partial and positional right nephrostomy tube kink (A: Standard Xrays; B: Positional Xrays). A white arrow is pointing at the kink in the nephrostomy catheter,yes
PMC10638592,Figure_1,oa_package/b1/c4/PMC10638592.tar.gz,"['The surgeon utilized a three portal technique: anterior, posterior, and bursal (indirect) arthroscopic portals to access the ACJ (', ' 1Intraoperative image demonstrating anterior, posterior, and bursal portal sites.']","Figure1 Intraoperative image demonstrating anterior, posterior, and bursal portal sites.",yes
PMC11021087,Figure_4,oa_package/57/f8/PMC11021087.tar.gz,"['At two months follow-up, he had no neuro deficits and magnetic resonance imaging of the brain was suggestive of near-total excision of the epidermoid cyst with resolving left parafalcine frontoparietal bleed [].', 'Magnetic resonance venography was suggestive of a patent superior sagittal sinus [].', ':(a-f) At two months follow-up, magnetic resonance imaging of the brain suggestive of near total excision of the epidermoid cyst with resolving left parafalcine frontoparietal bleed.']","Figure 4: (a-f) At two months follow-up, magnetic resonance imaging of the brain suggestive of near total excision of the epidermoid cyst with resolving left parafalcine frontoparietal bleed. Magnetic resonance venography was suggestive of a patent superior sagittal sinus.",yes
PMC10644142,Figure_21,oa_package/c9/5c/PMC10644142.tar.gz,[],"Figure 21 Stener lesion. (A) Longitudinal ultrasound in a 57-year-old female skier showing a yoyo (circle) on string (small arrows) corresponding to the retracted UCL and adductor aponeurosis respectively. (B) Coronal FS-T2-WI in another middle-aged skier shows a retracted and entrapped UCL (red arrows) proximal to the adductor aponeurosis (small arrows). Also note bone marrow edema at the ulnar side of the proximal phalanx. UCL, ulnar collateral ligament; FS, fat suppressed; WI, weighted image.",yes
PMC10803787,Figure_1,oa_package/35/27/PMC10803787.tar.gz,"['Pretreatment CT image.', 'CT images during the RFA procedure.']","Fig. 1 Pretreatment CT image. A nodule 20 mm in diameter (arrowhead) is seen on the mediastinal aspect of the left lung. The lesion abuts the aorta (A). FDG-PET/CT shows radionuclide uptake in the same lesion (B, arrowhead).",yes
PMC3731238,Figure_5,oa_package/31/8a/PMC3731238.tar.gz,"['N2 medium was completely changed after 24 hours and cells were harvested at different time points to analyse PrPSc accumulation ().', 'PrPSc was able to replicate in both cellular types since PrPSc was detected at 6 dpi for cells derived from the hippocampus (A) and 8 dpi for NSC derived from the lateral ventricles (B).', 'Infected KOPrP cells were used as negative control of the experiment in order to detect the remaining inocula (C).', 'g005Infection of NSC during differentiation.']",10.1371/journal.ppat.1003485.g005,yes
PMC11312861,Figure_7,oa_package/0b/0c/PMC11312861.tar.gz,"['Brooke Spiegler Syndrome (BSS)A 59-year-old female patient was referred for non-invasive examination and treatment of multiple skin-coloured tumours present in the head and trunk area (a,b).', 'The videodermoscopy enabled us to reveal of different aspects of cylindromas and helped rule out the presence of basal cell carcinomas (c).', 'The case of Brooke Spiegler syndrome: (a) clinical presentation multiple skin-coloured nodules within the area with a high distribution of sebaceous glands on the face; (b) clinical presentation multiple nodules of divert size, pink and skin-coloured, and solid in palpation, located on the trunk; (c) videodermoscopy reveals different aspects of cylindromas pinkish and yellowish structureless areas with blue globule aside of nodule, white blotches and strands on its surface, and short curved or irregular vessels mainly at the rim of nodule (upper left), structureless pink and white area with white strands and linear vessels (upper right), pink nodule with the centrally yellow structureless area, white strand and peripherally visible short linear vessels (lower left), larger nodule, pink and white in colour with large arborising vessels (lower right) (Fotofinder Medicam HD 1000 Systems GmbH, Bad Birnbach, Germany).']","Figure 7 The case of BrookeSpiegler syndrome: ( ) clinical presentationmultiple skin-coloured nodules within the area with a high distribution of sebaceous glands on the face; ( ) clinical presentationmultiple nodules of divert size, pink and skin-coloured, and solid in palpation, located on the trunk; ( ) videodermoscopy reveals different aspects of cylindromaspinkish and yellowish structureless areas with blue globule aside of nodule, white blotches and strands on its surface, and short curved or irregular vessels mainly at the rim of nodule ( ), structureless pink and white area with white strands and linear vessels ( ), pink nodule with the centrally yellow structureless area, white strand and peripherally visible short linear vessels ( ), larger nodule, pink and white in colour with large arborising vessels ( ) (Fotofinder Medicam HD 1000 Systems GmbH, Bad Birnbach, Germany).",yes
PMC9712280,Figure_1,oa_package/6f/76/PMC9712280.tar.gz,"['1).', 'Schematic illustration of the important neuroanatomical structures investigated in this study.', 'ResultsOlfactory bulb pathologyVantaa datasetOf the 124 Lewy-body positive cases in the Vantaa dataset, 27 were early cases with pathology in only 1 3 CNS locations (Table 1).', '1.']","Fig. 1 Schematic illustration of the important neuroanatomical structures investigated in this study. Amygdala-predominant cases had Lewy pathology in 13 structures predominantly within the red circle (in all cases including the amygdala), but limited or no pathology in brainstem or autonomic nervous system. In the brainstem-/PNS-predominant category (blue ellipse and circle), most cases had Lewy pathology in 13 structures in the brainstem (blue ellipse), except 9 cases with pathology restricted to the sympathetic trunk and/or heart (blue circle). AMY amygdala, CING cingulum, DMV dorsal motor nucleus of vagus, LC locus coeruleus, NBM nucleus basalis of Meynert, OB olfactory bulb, SN substantia nigra, SP-SA sacral spinal cord, SP-TH thoracic spinal cord, TOX transentorhinal cortex.",yes
PMC10206383,Figure_3,oa_package/6a/c8/PMC10206383.tar.gz,"['Clinical progression and development of peritonitis on day 3 (A D) Consecutive axial postcontrast abdominal CT images from superior to inferior show intraabdominal free fluid (arrows, A, B, and C), peritoneal thickening (arrowheads, A, B, and D), extensive pelvic fat stranding (D), and bilateral cystic adnexal lesions with enhancing thick walls (asterisks, C and D).', 'Because of the clinical progression and radiological findings, initial antibiotherapy was switched to the meropenem + vancomycin combination with broader-spectrum coverage.']","Fig. 3 Clinical progression and development of peritonitis on day 3 (AD) Consecutive axial postcontrast abdominal CT images from superior to inferior show intraabdominal free fluid (arrows, A, B, and C), peritoneal thickening (arrowheads, A, B, and D), extensive pelvic fat stranding (D), and bilateral cystic adnexal lesions with enhancing thick walls (asterisks, C and D).",yes
PMC10591112,Figure_3,oa_package/a0/a9/PMC10591112.tar.gz,['Role of artificial intelligence in the annotation of dental X-rays.'],"Figure 3 Role of artificial intelligence in the annotation of dental X-rays. (a) Segmentation of the different tooth structures and (b) details on the penetration level of the tooth decay. Image Credits: Dirk Neefs, DD-Care, Belgium",yes
PMC8665300,Figure_2,oa_package/15/76/PMC8665300.tar.gz,"['On examination, we noted a tyndall in the anterior chamber, an intraocular pressure of 38 mm Hg; more numerous goniosynechia over 360 ; a vitreous tyndall, blood vessels in the superior temporal region of the fundus and an optic atrophy ().', 'Disinhabited blood vessels temporally superior to the fundus.', 'On otoscopic examination, a pocket of antero-inferior retraction was found in the right ear; the eardrum had a purplish appearance.']",Fig. 2 Disinhabited blood vessels temporally superior to the fundus.,yes
PMC8529809,Figure_2,oa_package/f7/39/PMC8529809.tar.gz,"[' 2).', 'DL accurately identifies tissue regions and AT8-stained aggregates.', 'b Aggregate segmentation: twofold cross validation was used and performance is measured in terms of fraction of pixels that are correctly classifiedWe first trained DL models to segment tissue regions as cortex, WM or background, based on pathologist demarcations of these regions in 37 AT8 stained WSI.', ' 2a).', ' 2b, Additional file 1: S3a).']","Fig. 2 DL accurately identifies tissue regions and AT8-stained aggregates. For both region and aggregate models, a cross-validation scheme was used to generate multiple models, each trained on part of the data and evaluated on the rest. We report performance (left column) using confusion matrices which show how image areas with known true labels (rows) are assigned to different predicted classes (columns). Values denote fractions of patches/aggregates belonging to true class (i.e., rows add up to one) assigned to corresponding predicted class averaged across cross-validation models, with standard deviations shown in parenthesis. Right column shows sample classification results. Note: BG=Background. Region segmentation: threefold cross validation was used and performance is measured in terms of fraction of image-patches from a region that are correctly classified (diagonal entries in dark blue). Aggregate segmentation: twofold cross validation was used and performance is measured in terms of fraction of pixels that are correctly classified",yes
PMC5717126,Figure_1,oa_package/71/20/PMC5717126.tar.gz,"[' 1.', '0)0 [0]0 [0]1 [5]1 [5]4 [21]11 [58]0 [0]0 [0]0 [0]2 [11]2 [11]2 [11]\nFBP filtered back projection, HIR hybrid iterative reconstruction, MIR model-based iterative reconstruction, BR body routine, ST soft tissue, Obs observer\nExample of decreased sensitivity for stone detection.', 'From left to right the stone at routine dose reconstructed with FBP (a), the FBP reconstruction at the lowest dose level on which the stone was missed (b) and the IR reconstructions (HIR, MIR Body Routine and MIR Soft Tissue) at the same dose level on which the stone is clearly visible\nExtra-urinary tract pathologyResults of the assessment of extra-urinary tract pathology are displayed in Table 2.', ' B-C).', 'gif""/>Effects of KUSs on ATPase activities, ATP levels, and acetyl-CoA levels.']","Figure 3 Effects of KUSs on ATPase activities, ATP levels, and acetyl-CoA levels. (a) Inhibition of ATPase activity in clarified whole cell lysates by KUS121, KUS187, and KUS94, but not KUS11. Total ATPase activities in clarified whole cell lysates from differentiated PC12 cells were measured in the absence and presence of KUSs. Relative ATPase activities are shown with values in the absence of KUS (D: DMSO) set at 100%. (b) HeLa cells were cultured in medium with low glucose (0.25g/l) for 20hours, with or without KUSs, and ATP levels were measured with luciferase assays. (c) HeLa cells were cultured in medium with low glucose (0.25g/l) for 20hours, with or without KUSs, and acetyl-CoA levels were measured. * < 0.05, ** < 0.01, *** < 0.001 vs. DMSO control (Dunnett's test, n = 3).",yes
PMC9527564,Figure_1,oa_package/af/01/PMC9527564.tar.gz,['Magnetic resonance imaging scan sagittal view.'],Figure 1 Magnetic resonance imaging scan sagittal view. Revealed multiple level of cervical spine degenerative changes more at C5/6 causing sever canal stenosis and myelomalacia.,yes
PMC11657319,Figure_7,oa_package/e0/e5/PMC11657319.tar.gz,"['65%\nMicroscopic view of a spinal metastasis from a moderately differentiated adenocarcinoma of the right colon: (A) At the edge of the vertebral tumor there were small areas of carcinoma made of moderately differentiated gland with marked desmoplasia, osteolytic changes of the vertebral bone in the upper right corner; (B) In the center of the tumor there were sheets of cells with a cribriform pattern; small gland lumen was filled with necrotic debris (dirty necrosis) (arrow); (C) Tumor cells exhibited strong cytoplasmic positivity for CK AE1/AE3; (D) Tumor cells exhibited strong cytoplasmic positivity for CK20; (E) Tumor cells exhibited strong nuclear positivity for p53; (F) Tumor cells exhibited a very high Ki67 LI demonstrating an aggressive evolution (anti-Ki67 antibody, x40).', 'CK: Cytokeratin; HE: Hematoxylin Eosin; LI: Labeling index SMs originating in BCs exhibited two histological types: infiltrating ductal carcinoma, not otherwise specified (NOS) in 10.']","Figure 7 Microscopic view of a spinal metastasis from a moderately differentiated adenocarcinoma of the right colon: (A) At the edge of the vertebral tumor there were small areas of carcinoma made of moderately differentiated gland with marked desmoplasia, osteolytic changes of the vertebral bone in the upper right corner; (B) In the center of the tumor there were sheets of cells with a cribriform pattern; small gland lumen was filled with necrotic debris (dirty necrosis) (arrow); (C) Tumor cells exhibited strong cytoplasmic positivity for CK AE1/AE3; (D) Tumor cells exhibited strong cytoplasmic positivity for CK20; (E) Tumor cells exhibited strong nuclear positivity for p53; (F) Tumor cells exhibited a very high Ki67 LI demonstrating an aggressive evolution (anti-Ki67 antibody, x40). HE staining: (A) 100; (B) 200. Anti-CK AE1/AE3 antibody immunomarking: (C) 400. Anti-CK20 antibody immunomarking: (D) 100. Anti-p53 antibody immunomarking: (E) 400. Anti-Ki67 antibody immunomarking: (F) 400. CK: Cytokeratin; HE: HematoxylinEosin; LI: Labeling index",yes
PMC5688437,Figure_14,oa_package/f3/5b/PMC5688437.tar.gz,[],Fig. 14 Radiographs of a cat from Group B: Day 240. (See Fig. caption for details),yes
PMC2291447,Figure_3,oa_package/cf/a8/PMC2291447.tar.gz,"['However, to formally test the role of nTreg, mice were treated with a cocktail of anti-CD25 antibodies (previously shown to give optimal depletion of CD4 CD25hiFoxp3 cells; 25) 3 days prior to infection with PyL ().', 'Nonetheless, neither 7D4 treatment nor combined 7D4 PC61 treatment significantly altered the course of parasitaemia, anaemia or survival of PyL infection in C57BL/6 mice (A C).', 'g003Natural Treg do not significantly contribute to the virulence of PyL infection.', 'The proportion of Foxp3 cells fell from 10 15 in unsorted CD4 T cells to 1 2 in the CD25 CD4 population, whereas CD25 cells were highly enriched for Foxp3 cells (70 80 ; D).', '1000004-Couper1"" ref-type=""bibr"">[22], we found that control (unreconstituted) RAG / mice succumbed to PyL infection with the same kinetics as WT mice (compare E, F with ).', 'Furthermore, the course of infection was virtually indistinguishable in RAG / mice reconstituted with CD4 CD25 , CD4 CD25 or a 10 1 ratio of CD4 CD25 /CD4 CD25 T cells (E, F).']",10.1371/journal.ppat.1000004.g003,yes
PMC4059305,Figure_1,oa_package/b4/01/PMC4059305.tar.gz,"['brasiliensis in tissue fragments ().', 'Histological section of a lesion in the oral mucosa stained with Gomori-Grocott.']",Figure 1 Histological section of a lesion in the oral mucosa stained with Gomori-Grocott. Black dots indicate the presence of multiple budding blastoconidia characteristic of (400x).,yes
PMC8403399,Figure_2,oa_package/d1/e4/PMC8403399.tar.gz,"['Relative motilities of molecular mass markers are indicated on the left side of each blotOther Syn antibodies2H6, 3H11 and 94-3A10 are mouse monoclonal anti- Syn antibodies with the epitopes including residues 2 21, 43 62 and 130 140, respectively [12, 13].', ' 2) and show that they demonstrate minimal cross-reactivity to other proteins in soluble extracts from human brain (Additional file 1: S2).']","Fig. 2 Western blot analyses to demonstrate the specificities of new monoclonal Synantibodies. Immunoblot analyses was performed using 1102, 1103, 1115, 1119, 1122, 1125, 1129, and full-length (FL; 1140) recombinant Syn protein as indicated above each lane and as described in Material and Methods. 210ng of recombinant protein was loaded per lane and membranes were probed with the primary antibodies listed above each panel. Relative motilities of molecular mass markers are indicated on the left side of each blot",yes
PMC3447430,Figure_13,oa_package/db/09/PMC3447430.tar.gz,[],Figure 13. Follow-up CT after surgery. MPR reconstruction.,yes
PMC5679632,Figure_6,oa_package/5d/ef/PMC5679632.tar.gz,"['Here we compared mutations in these regions of cMyBP-C and the three domains of -MHC, including motor, actin-binding, and coiled-coil/rod (), to echocardiographic outcome.', 'g006Schematic diagram of cardiac -myosin heavy chain ( -MHC) and cardiac myosin binding protein-C (cMyBP-C) proteins.', 'Thus, the M domain allows force transmission on the Z-lines and, in turn, the whole myocyte for contraction ().', 'To achieve this, cMyBP-C can be broadly divided into three functional clusters: C0-C2 that can differentially interact with the myosin motor heads and actin filament, C3-C6 that acts as a neck/tether, and C7-C10 that intertwines with -myosin coiled-coil tail to anchor in the thick filament ().']",10.1371/journal.pone.0187948.g006,yes
PMC4195840,Figure_14,oa_package/19/f3/PMC4195840.tar.gz,[],"Fig. 14 Axial PET/CT image demonstrates slight asymmetrical uptake in the anatomic location of the longus colli muscles ( ) in a patient previously treated by right partial parotidectomy and radiation therapy for a lymphoepithelial SCC of the right parotid gland. Note hypometabolism of the residual right parotid gland ( ) and focal uptake in the left parotid gland. Corresponding axial CECT ( ) and contrast-enhanced fat suppressed T1-weighted MR ( ) images illustrate a necrotic metastatic retropharyngeal lymph node on the right side ( ),mistaken on PET/ CT for minor prevertebral muscle activity and therefore yielding a false negative evaluation. Note the diffuse and heterogeneous enhancement of the remaining right parotid gland following surgery and radiotherapy ( ) better depicted on MRI as on CECT. There is no morphological abnormality in the left parotid gland. Left parotid gland uptake seen on PET/CT was attributed to compensatory hyperactivity",yes
PMC10453870,Figure_5,oa_package/88/e0/PMC10453870.tar.gz,"['001; ).', '01) in comparison to controls ().', 'Illustrative micrographs of FJC and TUNEL staining in rat brain sections from the DG area of the hippocampus.']","Figure 5 Illustrative micrographs of FJC and TUNEL staining in rat brain sections from the DG area of the hippocampus. ( ) Fluorescent microphotographs of the hippocampus show the control and MDG groups at low magnification in the first line, and the second line signifies the corresponding groups at high magnification (rectangles). Arrows indicate FJC/TUNEL positivity. Scale bars = 500 and 50 m; = 8 per group. ( ) The number of FJC+ neurons in the DG region of the hippocampus for the control and MDG groups. The FJC+ significance of differences in group means was assessed using an unpaired -test. The results are expressed as the mean SD for each group, with a sample size of = 8, *** < 0.001 compared to the control group. The coronal rat brain section depicted in ( ) has been redrawn from Paxinos and Watson [ ], illustrating the DG photographed area (red rectangle). ( ) The number of TUNEL+ neurons in the DG region of the hippocampus for the control and MDG groups. The TUNEL+ significance of differences in group means was assessed using an unpaired -test. The results are expressed as the mean SD for each group, with a sample size of = 8, ** < 0.01 compared to the control group.",yes
PMC8023876,Figure_3,oa_package/a2/c5/PMC8023876.tar.gz,"['0 mm diameter) were attached to the acetabulum (), part cranially of the cup, part in the ischium bone.', '.']","Figure 3. Phantom: sawbone of the hemipelvis, with the Allofit acetabular cup with a polyethylene liner (size 54cm diameter) with tantalum markers attached around the acetabular bone.",yes
PMC6290305,Figure_4,oa_package/e6/21/PMC6290305.tar.gz,"['Postoperatively, the patient did well without complication ().', 'Post-operative photo of the patient in the posterior view, demonstrating the final closureDISCUSSIONThe patient in this case had not accessed the health care system for over 20 years and initially presented to a new primary care physician for evaluation of this mass.']","Fig. 4 Post-operative photo of the patient in the posterior view, demonstrating the final closure",yes
PMC11429506,Figure_4,oa_package/ef/1c/PMC11429506.tar.gz,"['In particular, pathologists need to learn how to interpret evRCM images due to color differences in skin adnexal structures and lymphocytic infiltration, the frequent lack of reference to the epidermis, and artifacts ().', 'Comparison of a sclerodermiform BCC between evRCM and pathology.']","Figure 4 Comparison of a sclerodermiform BCC between evRCM and pathology. ( ) Due to the lower contrast and the lack of epidermis, it is difficult to distinguish between sclerodermiform tumor nests and skin adnexal structures in the evRCM images. ( ) The sclerodermiform BCC is easy to recognize on the histological slide.",yes
PMC11325563,Figure_5,oa_package/bc/43/PMC11325563.tar.gz,"[' 5).', '', ' 5Moulage of a squamous cell carcinomaTip 5: Be inclusive and not exclusiveWith an ever-growing movement to promote Equality, Diversity and Inclusion (EDI) and decolonise the health profession curricula, moulage provides an excellent opportunity to review and evaluate how we assess various dermatological conditions [15, 16].']",Fig.5 Moulage of a squamous cell carcinoma,yes
PMC7557710,Figure_2,oa_package/5d/32/PMC7557710.tar.gz,"['This is probably explained by the fact that the patients with chronic PMVTs more frequently displayed an impaired venous inflow (), since 12/13 had no visible native branches of the SMV on CT prior to recanalization and TIPS.', '.', '1177_2058460120964074-fig2""/>The results of the present study support the necessity of placing a TIPS to maintain portal flow after PVT recanalization also in patients without cirrhosis.']","Fig. 2. Preoperative computer tomography (CT) and periprocedural portography images of a patient in whom reocclusion occurred (a), and of another patient with subsequent good patency (b), demonstrating the importance of venous inflow to the transjugular intrahepatic portosystemic shunt (TIPS).",yes
PMC6834541,Figure_4,oa_package/54/20/PMC6834541.tar.gz,"['01 for each of the brain tissue controls in unpaired Student s t test; total number of samples n = 12, n = 4 per tissue; C57BL6 (C57) or FVB mouse tissue was the control for Ki91 mouse tissues; error bars: SEM)Table 3Table summarizing qPCR validation of RNAseq results using brain tissue from 2- and 4-month-old Ki91 SCA3/MJD versus two control mouse strains (C57BL/6 and FVB)GeneTissuep value C57 vs SCA3Fold change C57 vs SCA3p value FVB vs SCA3Fold change FVB vs SCA354], the protection resulting from treatment with the SMA was associated with SF hypo-responsiveness, in terms of spontaneous, IL-1 and BLP-stimulated IL-6 release, ex vivo\n(M).', 'g008Exposure of CIA-mice to SMA 12b in vivo results in SF hypo-responsiveness ex vivo.']",10.1371/journal.ppat.1010069.g008,yes
PMC4531783,Figure_6,oa_package/d8/8a/PMC4531783.tar.gz,"['Renal pathologyMacroscopically, no pathological changes where observed in the kidneys apart smaller size of kidneys from mice fed with MCD diet (A).', 'Detailed analyses of Periodic Acid Schiff s (PAS) and Acidic Fuchsin Orange G (AFOG) stainings did not reveal any obvious pathological alterations in glomeruli (B,D), tubulointerstitium (', '6C) or vessels (C with asterixes).', 'No pathological infiltrates of F4/80 positive cells were observed in any of the groups apart from a significant increase in F4/80 positive cells in ApoE / mice fed with MCD diet (Table 2 and E).', 'Similarly, collagen type I immunohistochemistry confirmed the findings from histology with no observable fibrosis in neither of the groups apart from significantly increased deposition in ApoE / mice fed with MCD diet (Table 2 and F).', 'org/1999/xlink"" xlink:href=""srep12931-f5""/>Kidney pathology in wt and ApoE / mice with or without WD or MCD diet.']","Figure 6 Kidney pathology in wt and ApoE mice with or without WD or MCD diet. Kidney morphology and detailed analyses of ( , ) Periodic Acid Schiffs (PAS) and ( , ) Acidic Fuchsin Orange G (AFOG) stainings did not reveal any obvious pathological alterations in glomeruli ( , ), tubulointerstitium ( ) or vessels (asterixes in ). No macroscopic differences were observed between the groups except of smaller kidney size in mice fed with MCD diet. ( ) Immunohistochemistry for collagen type I, as a fibrosis marker and ( ) F4/80, as a marker for renal monocyte/macrophage/dendritic cells showed normal findings comparable to wildtype mice fed with normal chow in all groups, apart from increased inflammation and fibrosis in ApoE mice fed with MCD diet. Scale bars are 2.5mm for A, 25m for B,D and 50m for C,E,F.",yes
PMC3988139,Figure_3,oa_package/49/ae/PMC3988139.tar.gz,"['Mouse susceptibility to hydrosalpinx induction is independent of live organism recovery from the lower genital tract or mouse H-2 haplotypesTo search for factors or correlates associated with the mouse susceptibility to hydrosalpinx development, we compared both live chlamydial organism shedding time courses and mouse H-2 haplotypes among the 11 strains of mice ().', 'g003Live C.', 'The live organism shedding was monitored post infection as described in legend and expressed as Log10 IFUs (shown along the Y-axis of each panel).']",10.1371/journal.pone.0095076.g003,yes
PMC8276319,Figure_2,oa_package/d9/b5/PMC8276319.tar.gz,"['Follow-up cardiac computed tomography angiography was performed after 3 months, showing resolution of the dissection ().', 'Curved multiplanar reconstruction on cardiac computed tomography with contrast 3 months after invasive coronary angiography showing resolution of the spontaneous dissection of the left internal mammary artery bypass graft.']",Figure 2 Curved multiplanar reconstruction on cardiac computed tomography with contrast 3months after invasive coronary angiography showing resolution of the spontaneous dissection of the left internal mammary artery bypass graft.,yes
PMC11543643,Figure_2,oa_package/e2/2b/PMC11543643.tar.gz,"[' 1a and b) and a computed tomography scan (CT) (', ' 2Preoperative (a) axial and (b) coronal images of CT scan two years after first ballistic injury showing femoral neck nonunion (white arrow) and avascular necrosis of the femoral head (red arrow).']","Figure2 Preoperative (a) axial and (b) coronal images of CT scan two years after first ballistic injury showing femoral neck nonunion (white arrow) and avascular necrosis of the femoral head (red arrow). CT, computed tomography.",yes
PMC7666474,Figure_9,oa_package/c1/87/PMC7666474.tar.gz,"[' 9).', '', 'Of note, type 3 MNV are very difficult to visualize on en face and often appear only as small, white tufts as depicted in the above example (e)Visualization of MNV on OCTA is helpful for diagnosis and classification.']","Fig.9 Macular neovascularization types. 3x3mm en face images and corresponding B-scans for the 3 MNV types seen in wet AMD. Segmentation was manually adjusted to reveal the best en face view for each lesion. , Type 1 MNV: distinct vascular inlets at the level of the choriocapillaris are seen on en face ( ), B-scan reveals flow below the RPE ( , yellow arrow). , Type 2 MNV: en face view shows vascular branching suspicious for an MNV ( ), B-scan shows flow between the retina and the RPE ( , yellow arrow). , Type 3 MNV: en face view shows a small bright tuft ( , white arrowhead), B-scan view shows intraretinal flow trailing downwards, towards the choriocapillaris ( , yellow arrow). Of note, type 3 MNV are very difficult to visualize on en face and often appear only as small, white tufts as depicted in the above example ( )",yes
PMC6588579,Figure_1,oa_package/66/a7/PMC6588579.tar.gz,"[' 1, top left), and employs a droplet-flow regime which gives optimal temporal response in microfluidic analysis systems19 22.', ' 1 shows the simplest fluidic setup where analysis is via a simple mix-and-read colorimetric assay.', 'Schematic diagram illustrating the operation of the device.', 'An image illustrating a sensor being used in a clinical setting to monitor tissue is shown top-leftTraditionally, microdialysis utilises low perfusion flow rates (0.', ' 1, detailed design shown in Supplementary ', ' 1) as opposed to the more typical planetary rollers24, to allow much slower flow rates at a given motor speed important for maximising diffusion time across the probe membrane and boosting recovery.', ' 1 and shown in detail in Supplementary ', ' 1) and reacts to yield a red/purple coloured product which is quantified by a downstream flow cell31 as an absorbance measurement (']","Fig. 1 Schematic diagram illustrating the operation of the device. The screw-driven peristaltic pump simultaneously feeds perfusate into a microdialysis probe and withdraws the resulting dialysate into the device. From the pump, dialysate is delivered into a microfluidic chip, where an analyte-specific reagent is added, and the resultant flow immediately segmented into a stream of droplets by the addition of an immiscible oil. Within the droplets, the reagent reacts with the analyte to produce a measurable optical response. The droplets flow out of the chip into low-volume PTFE tubing and downstream to an optical flow cell where the product of the reaction is quantified . A microcontroller (not shown) saves the result to a micro SD card and relays it via Bluetooth to an external device. The analysed droplets are collected in a waste sachet for later disposal. An image illustrating a sensor being used in a clinical setting to monitor tissue is shown top-left",yes
PMC2776973,Figure_3,oa_package/29/d6/PMC2776973.tar.gz,"['Cells were then re-suspended in fresh, rich media containing extra sulfur metabolites and their cell cycle progression was monitored (A).', 'The reported growth rates (B) are the summary of many repeats of individual experiments (20% of change in shape, size and height of vertebral body. ( ). Normal vertebrae (L2) without pathological changes (green lines). T, thoracic vertebrae; L, lumbar vertebra.",yes
PMC8564909,Figure_7,oa_package/b8/d1/PMC8564909.tar.gz,"['Consistent with these findings, pretreatment of keratinocytes with a cPLA2 inhibitor (AACOCF3) or sPLA2 inhibitor (LY333013 and S3319) suppressed induction of proinflammatory genes (IL1B, CXCL1, and CCL20) and skin barrier genes (S100A7 and IVL) by TNF plus IL-17A stimulation (Supplemental , A and B).', 'Application of a topical sPLA2 inhibitor over a 5-day period resulted in decreased ear thickness, epidermal thickness, and inflammatory infiltrates (, A C).', 'qRT-PCR analysis of inflamed skin from IMQ mice treated with the sPLA2 inhibitor revealed significantly lower mRNA expression of proinflammatory genes (Il1b, Il36g, Defb4, and Ccl20) and differentiation-related genes (Dsg1, Krt1, Krt16, and Ivl), in addition to suppression of Pla2g2f expression itself (Supplemental 0) compared with control-treated mice (D).', 'Furthermore, similar responses were seen in cPLA2 inhibitor treated IMQ mice, including decreased ear thickness, acanthosis, and inflammatory cell infiltrates (, E H).', 'However, the impact on gene expression was more modest in cPLA2 inhibitor treated IMQ mice compared with sPLA2 inhibitor treatment, and was limited to differentiation and antimicrobial genes, including Defb4, Dsg1, Krt1, Krt16, and Ivl (H).', 'PLA2 inhibition alleviates psoriasis-like inflammation in vivo.']","Figure 7 PLA2 inhibition alleviates psoriasis-like inflammation in vivo. Chemical inhibitors targeting sPLA2 and cPLA2 were topically applied to IMQ-induced psoriasis-like mice ( = 5) to block PLA2 function. ( ) H&E staining of ear sections from IMQ-induced mice with sPLA2 inhibitor treatment on day 6. Scale bar: 50 m. ( ) Dynamic changes in ear thickness at the indicated time points. ( ) Epidermal thickness was evaluated based on the data in . ( ) qRT-PCR results showing mRNA expression of various cytokines and differentiation-related genes in the ears of IMQ mice on day 6. ( ) H&E staining of ear sections from IMQ-induced mice with sPLA2 inhibitor treatment on day 6. Scale bar: 50 m. ( ) Dynamic changes in ear thickness at the indicated time points. ( ) Epidermal thickness was evaluated based on the data in . ( ) qRT-PCR results showing mRNA expression of and differentiation-related genes in the ears of each indicated group on day 6. Two-way ANOVA. Data are presented as mean SEM. * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001.",yes
PMC10339210,Figure_5,oa_package/a4/01/PMC10339210.tar.gz,"['In this situation, engineered meshes have been developed by making the non-absorbable meshes as the basis and combining one or more synthetic or natural components such as ePTFE, polyvinylidene fluoride (PVDF), PLA, PGA (', '', 'In most situations, a functional hydrogel can be coated onto the hernia mesh to prevent the visceral adhesions (', 'Direct drug loading onto the hernia meshes is one of the practical approaches to achieving anti-bacterial functionalities (', 'Extracellular matrix components, biocompatible hydrogels (', ' 5A), fibrous membranes (', ' 5C), and functional agents (', 'To command post-implantation foreign body reactions, biologically derived components are subsequently investigated by various research groups hoping that cells should enhance the bio-compatibility and reduce foreign body reactions (']","Fig.4 Adsorbable hernia meshes. A. Surgical procedure performed for large-size abdominal wall defect reconstruction with a poly- -dioxanone mesh [ ]. B. Schematic diagram of a heat-shrinkable electrospun fibrous tape for reconstructing soft tissue both structurally and functionally in a rat abdominal wall hernia [ ]. C. Representative images of Masson's trichrome and dystrophin staining images of explanted grafts after 30 days of implantation. The human acellular collagen matrix group showed no adhesion with or without MSCs. There were different angiogenesis and musculogenesis between groups regarding the matrix loading with different cells [ ]. D. Schematic illustration of the small intestinal submucosa (SIS) membrane that modified with fusion peptide-mediated extracellular vesicles which can promote cell migration and spreading, achieving a more successful abdominal wall tissue regeneration [ ]. E. Representative pictures of the decellularized and crosslinked bovine pericardial implants, the full-thickness abdominal wall defects induced by sectioning ventral muscular tissue, and the defect reconstructing with implant [ ].",yes
PMC5946008,Figure_6,oa_package/c1/50/PMC5946008.tar.gz,['Six randomly selected examples of the conversion from sagittal to coronal radiographic projections of the trunk.'],Figure 6 Six randomly selected examples of the conversion from sagittal to coronal radiographic projections of the trunk. input: label data provided as input; output: image created by the generative model; target: ground truth.,yes
PMC9526636,Figure_4,oa_package/5b/7a/PMC9526636.tar.gz,"['003"" position=""float""/>SYVN1 controls ubiquitination and proteasomal degradation of Drp1 in KGN cells.']","Figure 4 SYVN1 controls ubiquitination and proteasomal degradation of Drp1 in KGN cells. (a) KGN cells were either transfected or not transfected with empty or SYVN1 overexpression plasmids, and western blotting was performed to examine protein expression of SYVN1 and Drp1 ( = 3). < 0.01 and < 0.001. One-way ANOVA with Tukey's multiple comparison test. SYVN1: (2, 6) = 75.35, < 0.0001. = 0.0001, < 0.0001; Drp1: (2, 6) = 24.44, = 0.0013. = 0.0017, = 0.0031. (b) Western blot analysis of the expression of SYVN1 and Drp1 in KGN cells transfected with control siRNA or SYVN1 siRNA ( = 3). < 0.05 and < 0.01. Two-tailed unpaired Student's -test. SYVN1: = 0.0049, = 5.625, df = 4; Drp1: = 0.0183, = 3.843, df = 4. (c) SYVN1 binding to Drp1 was detected by immunoprecipitation. (d) Western blotting was used to detect the effect of SYVN1 on Drp1 ubiquitin modification. (e) Western blot analysis of the expression of Drp1 in KGN cells transfected with either empty or SYVN1, in the presence or absence of the proteasome inhibitor MG132 ( = 3). All blots used -actin as loading controls. < 0.001. Two-way ANOVA with Sidak's multiple comparisons test. (5, 10) = 31.29, < 0.0001. (f) A cycloheximide (CHX) chase assay was performed to assess the half-life of Drp1. KGN cells were transfected with empty or SYVN1 for 24h. Cells were then treated with CHX (100 g/ml) for the indicated hours, and western blotting was performed. Relative Drp1 protein levels in KGN cells were quantified and plotted with the length (days) of CHX treatment on the axis. The data are expressed as mean standarddeviation.",yes
PMC10759054,Figure_3,oa_package/56/d5/PMC10759054.tar.gz,['Plain CT images and diagrams of the neck to chest.'],Figure 3 Plain CT images and diagrams of the neck to chest. The images show multiple rib fractures and extensive traumatic haemopneumothorax.,yes
PMC6820383,Figure_1,oa_package/d9/e6/PMC6820383.tar.gz,"['[3]Imaging techniquesChest X-ray is the initial investigation tool for PHC [].', '[12]Chest X-ray showing hydatid cyst of right lungThe radiological features of a PHC are sharply defined, round-to-oval homogenous opacity of variable size [].']",Figure 1 Chest X-ray showing hydatid cyst of right lung,yes
PMC2748684,Figure_3,oa_package/40/70/PMC2748684.tar.gz,"['The dramatic nearly complete elimination of pyramidal CA neurons was confirmed by Nissl staining (data not shown) and by IHC for NeuN as marker for neuronal nuclei (A, lower panels).', 'g003Dose-dependence and time-line of AAV-Tau.', 'Tau-mediated neurodegeneration is rapid and closely associated with microgliosisLower doses of AAV-Tau-P301L resulted in less neurodegeneration with marked thinning of CA1/2 in injected mice (A), coinciding spatially with expression of protein tau.', 'The relation was actually inverse: more extensive neuron-loss contrasted with less human tau (A), which is attributed to diminished or abolished protein synthesis in degenerating neurons.', 'At the later time-points neuro-degeneration was extensive also in the cortex (B, panels marked NeuN).', 'P301L injected mice (B, middle panels).', 'At later time-points, neuronal FJB staining decreased in parallel with NeuN (B), while reaction was also noted with micro- and astroglia, that were clearly activated and indicative of inflammation (B).', 'Inflammation was confirmed by IHC for activated microglia and astroglia (B).', '(B, utmost right panels marked MHCII).', 'Conversely, some astrogliosis was evident throughout the observation period (B, panels marked GFAP).', 'P301L mice (B).']",10.1371/journal.pone.0007280.g003,yes
PMC8120356,Figure_1,oa_package/2f/bb/PMC8120356.tar.gz,['Shear wave elastography.'],Figure 1 Shear wave elastography. Shear wave elastography was performed on the right lobe of the liver through intercostal spaces. A sample box of 2.02.0cm was placed. A 1.0cmdiameter circular region of interest was placed on the propagation map.,yes
PMC8715243,Figure_6,oa_package/78/e0/PMC8715243.tar.gz,"['7Patient #6 4R-Predominant PSP tauopathy Neuropathology, Steele-Richardson progressive supranuclear palsy syndromeMRI results in our standardized sampling regions identified a distinct pathological feature in primary motor cortex, which was notable for a large irregular hypointense smudge in the middle layers of cortex with some irregular extension of mild hypointensity into the subjacent WM relative to nearby gyri (, solid blue arrowheads), similar to GGT.', 'FTLD-Tau PSP 4R tauopathy (patient #6) primary motor MRI and pathology.', 'Corresponding to the deep-layer hypointensity on MRI, iron stain showed clustered deposits resembling activated microglia in layers III-V and adjacent WM (, solid blue arrowheads; ', 'On pathology, this was associated with a mixture of activated astrocytes and dystrophic microglia processes that were also evident in juxtacortical WM (, ).']","Fig. 5 FTLD-Tau GGT 4R tauopathy (patient #5) primary motor MRI and pathology. T2*w MRI of the inferior aspect of the primary motor region (including sampled BA 4). low-magnification (1x) view of tissue sample stained for iron (Fe) and myelin (LFB), with corresponding T2*w MRI slice. high-magnification (20x) tissue sample stained for tau pathology (Tau), Fe, and LFB (scale bar=100m). Rows depict view in upper cortical layers (GM II-III), deep cortical layers (GM IV-VI), directly adjacent white matter enriched for cortical U-fibers (WM-U) and relatively deeper white matter (WM) from boxes outlined in low magnification view (orange box=cortical layers, green box=deep WM). There is widespread tau pathology (black arrowheads, Tau) across cortical layers and in white matter along with severe neuronal loss and vacuolization across layers (LFB). T2*w MRI shows a large irregular hypointense band (blue arrow heads, T2*w) across mid to lower cortical layers. This coincided on histopathology with clusters iron deposits resembling hypertrophic appearing microglia (blue arrow heads, Fe). There was no apparent MRI signal corresponding to the band of Baillarger and adjacent white matter was striated with large hypointensities near blood vessels. This pattern corresponded on pathology to an absence of intracortical white matter and severe myelin loss in adjacent white matter, along with large clusters of iron deposits resembling glia surrounding larger blood vessels (blue arrow heads, Fe). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)",yes
PMC7805798,Figure_7,oa_package/a3/74/PMC7805798.tar.gz,"['Degeneration of dopamine neurons by wild-type -synuclein was very moderate in DAT-Cre;Oga+/+ mice, but the protective effect from Parkinson s disease-like pathology by upregulated O-GlcNAc was again evident in DAT-Cre;Ogafl/fl mice (Supplementary ).', '\nElevated O-GlcNAcylation mitigates impairments in dopamine transmission and motor learning caused by -synuclein.']","Figure 7 ( ) Schematic illustration depicting the injection of -synuclein AAV virus (A53T) into SNc (left hemisphere). ( and ) Quantification and summary statistics of dopamine release evoked by one pulse or five pulse stimulation in DLS from DAT-Cre;Ai32; and DAT-Cre;Ai32; mice injected with -synuclein (A53T) virus. ( ) Examples of movement path in the open field test in DAT-Cre; and DAT-Cre; mice injected with either PBS or -synuclein (A53T) virus. ( and ) Locomotive behaviour by total distance travelled and total moving time in the open field test. ( and ) Summary statistics for rearing behaviour in the cylinder test. ( ) Performances in the wire-hanging test. ( and ) Motor learning in the rotarod test during training days and test days. Blue bar indicates 450 nm light stimulation. Data were analysed by repeated measures two-way ANOVA followed by Sidaks test ( , , and ) and one-way ANOVA followed by Sidaks test ( and ). Data are represented as mean SEM. * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001.",yes
PMC9456133,Figure_1,oa_package/09/41/PMC9456133.tar.gz,"['hMIKO-1 Effectively Reduced Fibrosis in ILD LungAn increased collagen deposition caused fibrosis in the BLM-ILD lungs, and hMIKO-1 administration reduced this fibrosis (A).', 'The fibrosis score was significantly higher in the BLM-alone group than in the normal group but significantly lower in the BLM + hMIKO-1 group than in the BLM-alone group (B).', 'Collagen content was significantly higher in the BLM-alone group than in the normal group but significantly lower in the BLM + hMIKO-1 group than in the BLM-alone group (C).', '2144/00011272918476815Human MIKO-1 reduces lung fibrosis in the lungs of mice 28 days after bleomycin administration.']","Figure 1 Human MIKO-1 reduces lung fibrosis in the lungs of mice 28 days after bleomycin administration. ( ) Collagen deposition areas. Scale bars: 100 mm. ( ) Fibrosis scores. ( ) Collagen contents. Data are shown as mean SD (N = 10 mice per group). *** < 0.001, significant differences between the linked groups. NS: not significant.",yes
PMC8699760,Figure_5,oa_package/2d/1a/PMC8699760.tar.gz,"['The imagistic investigation was continued with a Chest, Abdomen and Pelvic CT Scan with IV contrast, which led to identification of multiple hepatic cysts (more than 30) ().', 'Abdominal CT Scan with IV contrast.']",Figure 3 Cranial computed tomography (CT) scans with intravenous (IV) contrast. ( ) axial; ( ) coronal.,yes
PMC6587644,Figure_2,oa_package/f9/93/PMC6587644.tar.gz,"['99mTc-sestamibi parathyroid (A, B) and autograft (C) dual-phase scan and SPECT/CT (CT images: D, G; SPECT images: E, H; fusion images: F, I) at 2 hours after administration indicated a nodule (1.']","Figure 2 Tc-sestamibi parathyroid (A, B) and autograft (C) dual-phase scan and SPECT/CT (CT images: D, G; SPECT images: E, H; fusion images: F, I) at 2hours after administration indicated a nodule (1.51.2cm) in suprasternal fossa and a nodule (2.51.0cm) in autografted site of right forearm, accompanied with intense radioactivity (arrow). SPECT/CT=single positron emission tomography/computed tomography.",yes
PMC7756068,Figure_5,oa_package/e1/42/PMC7756068.tar.gz,"['The C-terminal region of the SOX10 protein was predicted to be stabilized through interactions between the K2 and transactivation (TA) regions (A, left panel).', 'The frame shift region, shown in red, causes new contacts with the HMG domain, pushing one of the three helices into a more compact configuration (A, right panel).', 'Tyr460Leufs 42 mutation are less substantial compared to the Phe392Cysfs 117 variant (B).']","FIGURE 5 Structural models of Phe392Cysfs*117 and Tyr460Leufs*42 SOX10 frame-shift (fs) variants. The position of Phe392 and Tyr460 are indicated with arrows in the WT structure model (left panel). The positions of substituted residues are indicated with arrows in the mutant structures. Amino acid residues that result from the frame shift mutation are shown in red. Model of Phe392Cys fs*117 Sox10, the HMG domain is compacted due to the presence of the fs region and may impact DNA binding. The Tyr460Leu fs*42 model predicts a change in the TA structure with additional contacts between the TA/fs region and the HMG domain. This structural change may reduce activity of the TA region.",yes
PMC6617386,Figure_6,oa_package/df/44/PMC6617386.tar.gz,"['To test if this resistance can be modeled in mice, we assessed whether primary infection protects from secondary challenge (see schematic outline in 6A).', ' tyzzeri infection demonstrated significantly lower infection upon secondary challenge (s 6B and 6C shows a summary of multiple experiments, p 0.', 'To assess whether a cryptic infection contributed to the resistance here, mice were treated for 2 weeks with paromomycin to eradicate potential persisting parasites (see 6A in brackets).', 'Even after this drug treatment, previously infected mice were resistant to secondary challenge (s 6D, S4C, and S4D).', ' 6Infection Results in Protective Immunity That Can Be Induced with an Attenuated Vaccine(A) Schematic outline of challenge experiments for B, C, and D.', 'Mice that received parasites attenuated with irradiation at 300gray demonstrated significant protection when rechallenged with transgenic Ct-LC parasites (s 6E, 6F, and S5B S5F).', 'In contrast to wild-type C57BL/6, Rag1 and Ifn mice showed no protection from challenge following multiple vaccine exposures (s 6G and 6H).', 'These results underscore the central role of IFN and T cells in resistance to Cryptosporidium ( 6I) and they show that this natural model provides an experimental system to rigorously define the host and parasite factors required for a vaccine to generate the pathogen-specific T cells necessary to protect from cryptosporidiosis.']","Figure5 Interferon and T Cells Govern Susceptibility and Resolution of Murine Cryptosporidiosis (AC) Immunocompetent C57BL/6 mice infected with 10,000 oocysts of ( ) or ( ). Infection was monitored by whole animal imaging following parasite luciferase (A, quantified over entire infection in B) or by qPCR detecting parasite shed in the feces (C). Note that Cp produces robust infection in Ifn . For A and B, n= 9 mice total over 2 experiments, images shown are representative, and data represent mean SD with significance determined using a two-tailed t test ( p< 0.05). For C, n= 12 mice total, 4 per group, with qPCR performed on pooled fecal material with two technical replicates. (D) C57BL/6 wild-type mice (WT) and strains lacking IFN (Ifn ), mature B cells (Mt ), or mature B and Tcells (Rag1 ) were infected with 10,000 oocysts and infection was monitored by qPCR from collected feces. n= 16 mice total, 4 per group, with qPCR performed on pooled fecal material with two technical replicates, and data represent mean SD. (E) Homozygous (Nude) and heterozygous (Nude Hets) Foxn1 mice were infected with 10,000 transgenic oocysts and monitored for one month. n= 6 mice total; quantification of luminescence and fecal parasite shedding by qPCR in . Mice lacking Tcells (Rag1 and Nude) fail to clear the infection.",yes
PMC9426960,Figure_2,oa_package/5b/ce/PMC9426960.tar.gz,['The postoperative magnetic resonance imaging (MRI) scan showed that the external ventricular shunt was correctly placed and revealed non-communicating hydrocephalus due to probable extrinsic compression of the floor of the third ventricle by dolichoectasia of the basilar stem ().'],"Figure 2 Brain MRI (T1-weighted image with contrast, sagittal view) showing dolichoectasia of the basilar artery (yellow arrow)",yes
PMC9520103,Figure_5,oa_package/ec/4a/PMC9520103.tar.gz,"[' 5) and periodic-acid Schiff stains (PAS) [109].', ' 5).', 'Photomicrographs showing broad hyaline aseptate hyphae with irregular and right-angle branching, a H E 400; b Gomori methenamine silver 400; c d melanized hyphae and sporangia in tissue section (H E 400); e f melanin pigment in the fungal cell walls and sporangia confirmed and highlighted on melanin stain (Masson Fontana 400)Sporangia are more commonly reported in colonizing Aspergillus spp.']","Fig. 5 Photomicrographs showing broad hyaline aseptate hyphae with irregular and right-angle branching, H&E400; Gomori methenamine silver400; & melanized hyphae and sporangia in tissue section (H&E400); & melanin pigment in the fungal cell walls and sporangia confirmed and highlighted on melanin stain (Masson Fontana400)",yes
PMC10371051,Figure_4,oa_package/e5/29/PMC10371051.tar.gz,"['This supported a diagnosis of a high-grade sarcoma with epithelioid features consistent with epithelioid angiosarcoma (see ).', 'Surgical pathology.']",Figure 4 Surgical pathology. ( ) Surgical pathology showed pericardial thickening with ropy collagen fibres and tumour composed of epithelioid cells. ( ) Immunohistochemical staining showed positivity for endothelial markers found in sarcomas (cytokeratin and vimentin) and negative for epithelial markers seen in mesotheliomas (calretinin and Wilms' tumor 1 [WT-1]).,yes
PMC10644142,Figure_19,oa_package/c9/5c/PMC10644142.tar.gz,[],Figure 19 Tennis elbow with proximal extensor tendinopathy in a middle-aged tennis player. (A) Longitudinal gray scale ultrasound showing thickening and hypoechogenicity of the proximal extensor tendon. (B) Longitudinal ultrasound with power doppler shows hypervascularity of the affected tendon. (C) Longitudinal gray scale ultrasound showing a normal fibrillar structure of the extensor tendons (red arrows) in a normal individual for comparison with (A). No increased power doppler was seen (not shown).,yes
PMC4654517,Figure_4,oa_package/97/9c/PMC4654517.tar.gz,"['Bronchiolar epithelial lesions were severe at 1 dpi and characterized by marked degeneration, blebing of cytoplasm, necrosis of bronchial and bronchiolar epithelium (A).', 'Moderate to severe vacuolar degeneration and mild necrosis of bronchial and bronchiolar epithelial cells at hilus and almost healthy alveoli were the prominent findings (B).', 'About 80% of lining epithelial cells were positive for EIV antigen (C).', 'g004Pathogenicity of eq/J-K/08 H3N8 EIV in mice:Lung (H E):\nA: Early interstitial thickening with mild perivascular cuffing by inflammatory cells (arrow) at 1 dpi X100.', 'C: Degenerated and necrosed bronchial epithelial cells (C section) reveals EIV positive antigens in cytoplasm (arrow) at 12 hr post infection (IIPT) X400 (n = 6).', 'E: Immunohistochemical staining for EIV positive antigens (D section) in degenerated and denuded bronchiolar epithelial cells (arrow) and interstitial macrophages at 2 dpi (IIPT) X400 (n = 6).', 'g004""/>Vascular and alveolar parenchymal changes were severe at 2 dpi and characterized by severe degeneration and necrosis of bronchial epithelium, margination, perivascular cuffing of neutrophils and vascular congestion along with EIV antigens distributions in bronchial epithelium (D and 4E and 25 mm ). Contralateral ventricle volume (red arrowhead) was used as a measure of post-traumatic hydrocephalus severity. Threefold-to-tenfold enlargement of the contralateral ventricle occurred in 17/20 rats with TBI. Only 3/20 rats with lateral FPI had ventricle volumes corresponding to that in sham-operated experimental controls (3.10.4 mm at 1month, 3.30.4 mm at 3months, 3.50.4 mm at 6months). Ventricle enlargement was observed also in the lateral horn of the lateral ventricle (white arrow) and in the 3rd ventricle (open arrowhead)",yes
PMC10584764,Figure_7,oa_package/7a/e6/PMC10584764.tar.gz,"[' 7).', 'Instances that IVC filters are detected accurately (TP).', ', Table3)Table 2Filter candidate localization stage.', ' 7.']","Fig. 7 Instances that IVC filters are detected accurately (TP). Green bounding box (BB) is the ground truth, red BB is the prediction, and associated yellow text is the confidence of the prediction. The performance of detection stage is measured based on bounding boxes overlap (c.f., Table )",yes
PMC8299493,Figure_6,oa_package/6a/64/PMC8299493.tar.gz,"['\nImages need to be labeled appropriately indicating modality, plane of imaging, and specific window/sequence.']","Fig. 6 Images need to be labeled appropriately indicating modality, plane of imaging, and specific window/sequence. This would complement the oral presentation and will aid better time management. Ensure that every slide has a running title.",yes
PMC6584807,Figure_4,oa_package/a3/6b/PMC6584807.tar.gz,"['As shown in , treatment of neuronal cultures with A was found to increase p-Tau immunoreactivity in dendritic puncta (A,B).', 'The latter was abrogated when cultures were simultaneously exposed to Pio (A,B).', 'More detailed analysis of the data, in which the number of PSD-95/synapsin-positive synapses labeled with pTau were quantified, confirmed that Pio can effectively prevent the A -induced mislocalization of pTau in PSD-95/synapsin-positive puncta (C).']","FIGURE 4 Prevention of A-driven synaptic missorting of phospho-Tau by Pio. Representative immunofluorescent images of 14 DIV neurons stained with antibodies against pTau (pSer396-Tau, ) and PSD-95 ( ) after treatment with A (1 M, 24 h) and/or Pio (10 M). Examples of apposed PSD-95- and pTau-positive staining are marked with white arrows. Density of pTau-positive puncta in dendrites increases after exposure to A ( < 0.0001 vs. control) in a Pio-reversible fashion ( = 0.0002 vs. A alone) [1-way ANOVA, = 18.02; < 0.0001]. The percentage of PSD-95-labeled synaptic puncta that are pTau-positive is higher in A- vs. vehicle-treated cells ( = 0.0002), indicating missorting of pTau to synapses. In the presence of Pio, A exhibited a weaker effect on pTau missorting ( = 0.002) [1-way ANOVA, = 11.79; < 0.0001]; = 15 non-overlapping fields/condition; different cultures. Means S.E.M. are depicted. Scale bar: 10 m.",yes
PMC9634967,Figure_4,oa_package/29/0f/PMC9634967.tar.gz,"['Consequently, LYTL tubules are formed almost exclusively in the perinuclear area (B), where the ER is densely packed, as opposed to the peripheral ER, which forms a clear and distinct monolayer (A).', 'Thus, to ensure that the colocalization of the ER at the constriction site is not a random event, we compared the fluorescence intensity of the ER at the constriction site in the original picture and after the ER has been rotated 90o to the right (, C E) to randomize its position.', '7 fold) of the ER intensity in the constriction site in the randomized group compared with the original group (, D and E), indicating that the presence of the ER at the constriction site is not a random event.', 'To show fluorescence intensity in C and Supplemental S4, the Fire LUT was used.']",,yes
PMC10967391,Figure_4,oa_package/65/e6/PMC10967391.tar.gz,"['We performed scRNA-Seq on purified CD45-negative cells from 3 HS lesional skin specimens and 6 healthy skin specimens, which clustered into subsets composed of fibroblasts (marked by PDPN, DCN, and PDGFRA) and endothelial cells (marked by PECAM1, CDH5, and TIE1) (A).', 'Given the potential role of keratinocytes in HS pathology, we additionally sequenced CD45-negative cells from epidermal preparations of 2 HS specimens and 2 healthy skin specimens (B).', 'CellPhoneDB analysis among HS skin endothelial cell clusters, fibroblast clusters, keratinocyte clusters, and B cells identified numerous interactions between stromal cell receptors/ligands and B cells (C).', 'While most interaction partners expressed by endothelial cells were also expressed in healthy skin (Supplemental A), fibroblasts in HS skin upregulated numerous molecules versus healthy controls (D).', 'Expression of these factors was not confined to a single fibroblast cluster and was lower or absent from healthy skin (E).', 'Although keratinocytes also upregulated some chemokines in HS skin, these were limited to lower abundance subsets (Supplemental B).', 'As 3 separate groups of stromal cells were assessed with CellPhoneDB in , a more stringent P value (P 0.', 'Fibroblasts are primed to support and recruit B cells in HS skin.']","Figure 4 Fibroblasts are primed to support and recruit B cells in HS skin. ( ) UMAPs of stromal cell scRNA-Seq clusters from HS lesions and normal skin showing expression of endothelial and fibroblast signature genes. Data represent samples from 3 HS donors and 6 normal skin donors. ( ) UMAPs of scRNA-Seq data of epidermal cells from HS lesions and healthy skin showing expression of keratinocyte signature genes. Data represent samples from 2 HS donors and 2 normal skin donors. ( ) Significant ( < 0.005) ligand-receptor interaction partners identified by CellPhoneDB between clusters of stromal cells (left partner) and B cells (right partner). Numbers of clusters involved are normalized to the total number of clusters involved per cell type. B cell data were utilized from 5 HS donors, keratinocyte data from 2 HS donors, and endothelial and fibroblast clusters from 3 HS donors. ( ) Row-normalized heatmap depicting pseudobulk scRNA-Seq counts of significantly upregulated (adjusted < 0.05, Wald test) genes in HS fibroblast clusters versus healthy skin fibroblast clusters. Data represent samples from 3 HS donors and 6 normal skin donors. ( ) UMAPs of stromal cell scRNA-Seq clusters from HS lesions and healthy skin showing expression of select differentially expressed genes. Data represent samples from 3 HS donors and 6 healthy skin donors.",yes
PMC10972650,Figure_3,oa_package/93/df/PMC10972650.tar.gz,"['24\n25\nUninterrupted T2 hypointense junctional zone and early phase subendometrial enhancement are imaging signs of intact myometrium (\n\n).', 'org/1999/xlink"" xlink:href=""10-1055-s-0043-1777355-i2382906-2""/>\nEndometrial biopsy of a 57-year-old female with postmenopausal bleeding showed grade 1 endometroid endometrial carcinoma.']","Fig. 3 Endometrial biopsy of a 57-year-old female with postmenopausal bleeding showed grade 1 endometroid endometrial carcinoma. Magnetic resonance imaging high-resolution T2 weighted ( ) sagittal and ( ) axial images showed intermediate signal intensity mass in the endometrial cavity in the fundus region with the intact T2 hypointense junctional zone. ( ) Sagittal and axial early post-contrast images show uninterrupted smooth sub-endometrial enhancement consistent with tumor confined to the endometrial cavity. In line with the new staging system, this would be staged 1A1 endometrial cancer.",yes
PMC7600691,Figure_11,oa_package/8a/8a/PMC7600691.tar.gz,[],"Figure 11 Schematic of the PDMS-based microfluidic device consisted of a cell channel ( ), thin PDMS membrane ( ), and air channel ( ). Air pumped into the bottom channel deflects the PDMS membrane upwards to create a 3D constriction (artificial atherosclerosis plaque) in the stenosis region of the top channel. Adapted from Menon et al. [ ].",yes
PMC11412808,Figure_1,oa_package/3a/b8/PMC11412808.tar.gz,[],Figure1 68Ga-DOTA-NOC PET/CT scan before treatment showing the positive uptake in the pancreatic tail (SUV max 11.45). 68Ga-DOTA-NOC PET/CT scan after surgery showing no hyperfixation foci.,yes
PMC10998380,Figure_3,oa_package/02/71/PMC10998380.tar.gz,"[' 3a).', ' 3b).', 'Examples of generative SSL pretext tasksPredictive methodsMany custom pretext tasks have been proposed for computer vision that involve learning a specific transformation applied to images.']",Fig. 3 Examples of generative SSL pretext tasks,yes
PMC5108591,Figure_7,oa_package/8d/92/PMC5108591.tar.gz,"['RT-qPCR revealed that both Ret isoforms were expressed in primary murine satellite cells (A).', 'However, over-expression of either RET isoform significantly reduced caspase 3/7 release, implying that DUX4-mediated up-regulation of RET does not contribute to the apoptotic phenotype (B).', '10.', '008.', ' (G) Plot of probability that a cell has MyHC immunoreactivity derived from binomial models ( source data 1).', '009 source data 1.', '009DUX4 also suppresses Pax7 and myogenic regulatory factor (MRF) gene expression and inhibits proliferation and myogenic differentiation (Mitsuhashi et al.', 'Myoblasts transduced with control or DUX4 encoding retrovirus were additionally transfected with 20 nM control or Ret siRNA for 48 hr in proliferation medium and pulsed with EdU for two hours (C).', 'Knockdown of Ret did not rescue the reduced number of eGFP+/Pax7+ myoblasts in the presence of DUX4 (D).', 'We then tested whether knockdown of Ret by siRNA could rescue myoblast differentiation in the presence of DUX4 (E).', '06 10 4, E, F and G, source data 1).', 'Additionally although expression of Ret in satellite cell-derived myoblasts was not apoptotic (B), it was important to understand whether Sunitinib affects apoptosis in DUX4-expressing cells.']",10.7554/eLife.11405.008,yes
PMC5077970,Figure_2,oa_package/65/15/PMC5077970.tar.gz,"['Percentage of patients with distant lymph node metastases according to the number of positive lymph nodes in primary tumorSelection of patients with more than ten lymph nodes examined (N = 1024 pts; autopsy cohort = 258 pts; clinical cohort = 766 pts), p 0.']","Figure 2 Percentage of patients with distant lymph node metastases according to the number of positive lymph nodes in primary tumor Selection of patients with more than ten lymph nodes examined ( = 1024 pts; autopsy cohort = 258 pts; clinical cohort = 766 pts), < 0.001.",yes
PMC7666849,Figure_11,oa_package/67/bb/PMC7666849.tar.gz,[],Figure 11 Normal cerebral cortex. Cerebral cortex outermost layer showing different kinds of neuronal bodies; More internal part of the cortex where the integrity of the neuropil is observed. Hematoxylin and eosin stain.,yes
PMC5797461,Figure_3,oa_package/ef/a6/PMC5797461.tar.gz,"['14Table 1Comparison of the characteristics of pure GGNs and mixed GGNsGGN categorypGGNmGGNCommon pathology typeAAH, AIS, and MIAMIA, invasive adenocarcinomaPleural and lymphatic invasionRareMore frequentMicropapillary componentRareMore frequentCTR0Between 0 and 1Spiculated borderLess frequentMore frequentRecurrence rateLowerHigherAbbreviations: AAH, atypical adenomatous hyperplasia; AIS, adenocarcinoma in situ; CTR, consolidation/tumor ratio; GGN, ground-glass nodule; MIA, minimally invasive adenocarcinoma; pGGN, pure GGN.']","Figure 3 Schematic diagram of trailing method for deploying the microcoil. ( ) The distance between needle tip and outside the parietal pleura was measured and marked on the guide wire; ( ) the guide wire was inserted into the needle and advanced to the marked location. The distal part of the microcoil was deployed and coiled in the lung parenchyma; ( ) the guide wire was held in place, and the needle was withdrawn slowly. When the needle was withdrawn beyond the parietal pleura, the needle and guide wire were withdrawn simultaneously; ( ) the microcoil was deployed with the proximal part coiling beyond the parietal pleura and the distal part anchoring in the lung parenchyma. From Sui X, Zhao H, Yang F, Li JL, Wang J. Computed tomography guided microcoil localization for pulmonary small nodules and ground-glass opacity prior to thoracoscopic resection. . 2015;7:15801587. With permission from AME Publishing Company.",yes
PMC2853529,Figure_2,oa_package/57/c6/PMC2853529.tar.gz,['Early increase in serum IL-6 can be used to stratify TBI patients based on risk for developing elevated ICP.'],"Figure 2 . . Time course of ICP in patients classified as having elevated ICP (ICP > 25 mm Hg) versus that recorded in patients whose ICP remained below 20 mm Hg for the duration of the sampling period. . Standard curve showing the relationship between increasing IL-6 concentrations and the mean fluorescent intensity (MFI) detected by Luminex analysis. . Summary data showing the levels of IL-6 detected in the serum of healthy volunteers (HV), from TBI patients with elevated ICP (ICP > 25 mm Hg), and from patients whose ICP remained below 20 mm Hg throughout the study period. . Luminex data organized based on time of sample withdrawal relative to the subsequent increase in ICP. Day 0 represents the time point at which the ICP was recorded to surpass 25 mm Hg. Each patient with an elevation in ICP was paired with a patient whose ICP remained 20 mm Hg throughout the study period. Data is presented as mean SEM. , significant difference by two-way ANOVA.",yes
PMC10417292,Figure_2,oa_package/0b/61/PMC10417292.tar.gz,"['Tilting the perpendicular plane of the z-axis showed no relevant impact on the presence of the criteria defining the Darth Vader sign (), which was identified only in the spondylolysis case ().', 'Independence of the Darth Vader sign from plane angulation.']","Figure 2 Independence of the Darth Vader sign from plane angulation. Defects of the pars interarticularis (arrows) and the respective criteria of the Darth Vader sign are noted in axial (red), as well as in axial oblique of +20 (yellow) and 20 (green) degrees.",yes
PMC2721572,Figure_6,oa_package/20/3f/PMC2721572.tar.gz,"['The retained urine immediately dilutes the contrast that enters into the dilated left renal pelvis\naKUB, CT scan, Retrograde Pyelogram, Antegrade Nephrostogram and Rotational Fluoroscopic Tomography.', 'Image demonstrates extravasation of contrast from the distal right ureter\n(b-c)Axial sections of CT scan demonstrating normal ureteral anatomy in the abdomen (b), but the pelvic portion of the right ureter has contrast extravasating at the level of the right UVJ (c)\ndRetrograde pyelogram demonstrates complete separation of the distal right ureter from the UVJ\neAntegrade contrast study performed via right nephrostomy tube, demonstrating the atretic narrow lumen of the distal right ureter.', 'This study was performed in order to aid the preoperative planning\nfThree-dimensional rotational fluoroscopic tomography image demonstrating the anatomy of the distal right ureter in relationship to the bladder.', 'The radiation exposure of an RFT study is one-sixth that of a CT scan [f and 9b].']",Figure 3 VCUG. Male infant with prenatally detected bilateral hydronephrosis. VCUG demonstrates bilateral Grade 5/5 VUR. Additional information includes intra-renal reflux in the right kidney and also a prominent utricle with reflux of contrast into it. (a) VCUG with bilateral Grade 5/5 VUR and right intra-renal reflux. (b) VCUG demonstrating reflux into the utricle,yes
PMC11231514,Figure_2,oa_package/a0/92/PMC11231514.tar.gz,"['On magnification views performed for diagnostic mammography (), there are new groups of coarse heterogeneous and amorphous calcifications in the upper aspect of the right breast and a new group of coarse heterogeneous calcifications in the upper outer aspect of the left breast.', 'Multiple tissue cores were obtained from a single site of biopsy in the right and left breast using digital breast tomosynthesis with a 9 gauge vacuum assisted device.']",Fig. 2 Bilateral magnification views of calcifications. Craniocaudal (A) and mediolateral (B) magnification views of the right breast demonstrating new groups of coarse heterogeneous and amorphous calcifications in the upper aspect of the right breast (arrows). Craniocaudal (C) and mediolateral (D) magnification views of the left breast demonstrating a new group of coarse heterogeneous calcifications in the upper outer aspect of the left breast (arrow).,yes
PMC11128201,Figure_2,oa_package/77/4d/PMC11128201.tar.gz,"['HSPA12A downregulation contributed to MI/R injuryTo investigate whether downregulation of HSPA12A contributed to MI/R injury, we subjected HSPA12A-KO mice (Hspa12a / ) and WT littermates to MI/R surgery (A).', 'Ablation of HSPA12A in hearts of Hspa12a / mice was shown in B.', 'At basal (sham) levels, cardiac systolic function (EF% and FS%), left ventricle chamber size (left ventricular internal diameter at diastolic phase [LVIDd], left ventricular internal diameter at systolic phase [LVIDs], left ventricular end-diastolic volume [LVVd], and left ventricular end-systolic volume [LVVs]), and left ventricle wall thickness (interventricular septum thickness at diastolic phase [IVSd], interventricular septum thickness at systolic phase [IVSs], left ventricular posterior wall thickness at diastolic phase [LVPWd], and left ventricular posterior wall thickness at systolic phase [LVPWs]) were similar in Hspa12a / and WT mice (C and Supplemental s 2 and 3).', 'However, cardiac function measured 3 hours after reperfusion demonstrated that the MI/R-induced decreases of EF% and FS% were further exacerbated in Hspa12a / mice relative to WT mice (C).', 'Consistent with compounded cardiac dysfunction in Hspa12a / MI/R mice, infarct size was larger in Hspa12a / MI/R mice relative to WT controls (D).', 'Moreover, MI/R-induced cardiomyocyte apoptosis (TUENL+/ -actinin+) was increased in Hspa12a / mice relative to WT mice (E).', 'Downregulation of HSPA12A contributed to MI/R injury.']","Figure 2 Downregulation of HSPA12A contributed to MI/R injury. ( ) Mouse experimental protocol. ( ) The absence of HSPA12A expression in hearts of mice was demonstrated by immunoblotting. = 10 mice/group. ( ) Cardiac function was evaluated 3 hours after MI/R by echocardiography. = 6/group. ( ) Infarct size was examined 24 hours after MI/R. The infarct regions were shown as pale white with TTC staining. The ischemia risk area was illustrated by Evans blue nonstained areas. = 6 mice/group. ( ) Apoptosis in cardiomyocytes was examined 3 hours after MI/R by TUNEL assay on the paraffin-embedded sections that prepared from cardiac tissues at papillary muscles. -Actinin was used to stain cardiomyocytes, and DAPI was used to counterstain the nuclei. Scale bars: 50 m. = 5/group. Data are shown as mean SD. *** 0.001, ** 0.01, and * 0.05 by 2-way ANOVA followed by post hoc test ( and ) or Students 2-tailed unpaired test ( ).",yes
PMC11403037,Figure_7,oa_package/a8/c2/PMC11403037.tar.gz,[],Figure6 (a) Kaplan Meier estimate of renal survival after lung transplantation (months). (b) Kaplan Meier estimate of renal survival after lung transplantation by pulmonary diseases. Survivals were compared with the log-rank test ( = NS).,yes
PMC9437541,Figure_1,oa_package/e3/52/PMC9437541.tar.gz,"['Severity of PVS was defined as none/mild (rating scale 0 1), moderate (rating scale 2), and severe (rating scale 3 4) ().', 'Representative axial T2-weighted images of enlarged PVS in centrum semiovale (ePVS-CSO) and basal ganglia (ePVS-BG).']",Figure 1 Representative axial T2-weighted images of enlarged PVS in centrum semiovale (ePVS-CSO) and basal ganglia (ePVS-BG). Indicate ePVS-CSO with red squares corresponding to degree of severity. Indicate ePVS-BG with yellow squares corresponding to degree of severity.,yes
PMC5015953,Figure_6,oa_package/a4/10/PMC5015953.tar.gz,"['The BR1 horse expressed the wildtype transcript, but also a transcript that lacked 96 nucleotides (nt) consisting of the entire exon 10 and the first 20 nt of exon 11, confirming the existence of an aberrantly spliced MBTPS2 transcript in the BR1 horse ( and Source Data Unprocessed images and western blots.']","Fig. 4 EVs secreted by senescent BMMs propagate senescence and aging, whereas YBMM-EVs partially rejuvenate aging. , Experimental setup for EV transplantation. BMMs were isolated from young mice (3months old, male) and aged mice (20months old, male), and EVs secreted by BMMs were collected (YBMM-EVs, ABMM-EVs) and subsequently transplanted into young recipients (3months old, male mice). , Immunofluorescence staining for p53 in muscle (scale bar, 50m; =4 mice) (upper left), adipose tissue (scale bar, 50m; =4 mice; 56 images per mouse) (middle left) and bone sections (scale bar, 50m; =6 mice; 56 images per mouse) (bottom left) of young mice treated with YBMM-EVs or ABMM-EVs, and their quantitative analysis (right). , GTT and ITT were performed on young mice receiving adaptive transfer of EVs ( =6 mice). , Phosphorylation level of insulin signaling in liver (left) and quantitative analysis (right) ( =3 mice). , Representative CT images ( =6 mice). , Outline of the studies. BMMs were isolated from young mice (3months old, male) and aged mice (20months old, male), and EVs secreted by BMMs (YBMM-EVs, ABMM-EVs) were collected and subsequently transplanted into aged recipients (20months old, male mice). , Immunofluorescence detection of p53 in liver (upper left) and femur (middle left) (scale bar, 50m; =4 mice; 56 images per mouse) and H2A.X in muscle (scale bar, 50m; =4 mice; 56 images per mouse) (lower left) and brain (scale bar, 50m; =5 mice; 56 images per mouse) (bottom left) of aged mice after YBMM-EV or ABMM-EV transplantation, and their quantitative analysis (right). , Protein levels of senescence-related markers in liver (upper left), muscle (middle) and adipose tissue (upper right), and their quantitative analysis (bottom) ( =3 mice). , , GTT ( ) and ITT ( ) ( =5 mice). The colored shading in , and represents the area under the curve. , Representative CT images (left) and quantitative analysis (right) of bone volume/tissue volume (BV/TV) ( =5 mice). Data are presented as means.e.m. * <0.05, ** <0.01, *** <0.001 and <0.0001, as determined by one-way ANOVA followed by Tukeys multiple comparison test. mo, months;",yes
PMC3857475,Figure_4,oa_package/46/20/PMC3857475.tar.gz,"['Remarkably, endogenous APC, detected using two different antibodies, as well as GFP-APC (not depicted), are efficiently recruited into mutant Fus granules ( a; and ', 'The Wnt pathway components -catenin and Axin and the P-body marker Dcp1a were absent ( a).', '.', 'Similarly, APC was recruited into mutant Fus granules in primary neuronal cells ( b).', 'These inclusions are absent from healthy donors and, significantly, are also immunoreactive for APC ( c).', 'S1, e and f) was robustly recruited into mutant Fus granules, whereas the control RhoA mRNA was recruited to a much lesser extent ( d), indicating that APC-RNPs are preferentially affected by ALS-associated Fus mutants.']","Figure 4. (a) NIH/3T3 cells were transfected with GFP-Fus(R521C) (top panels). Middle panels show distribution of coexpressed fluorescently tagged proteins or immunostaining of endogenous proteins. (b) Primary hippocampal neurons were transfected with GFP or GFP-Fus(R521C) and were immunostained at DIV5 to detect APC. (c) Hippocampal sections from a patient with FTLD-Fus (sporadic NIFID) and a control donor were stained for Fus and APC. Nuclei were stained with Draq5. Arrows point to Fus granules. Note that likely both glial cells and neurons are present in these sections. (d) NIH/3T3 cells transfected with GFP- or RFP-Fus(R521C) were analyzed by FISH to detect the Ddr2 or RhoA mRNAs or were cotransfected with DsRed-MS2 and an MS2 reporter RNA carrying the Pkp4 3UTR (24bs/Pkp4). Graph shows relative fluorescence intensities of Ddr2 and RhoA mRNAs within the cytoplasm or Fus granules. P-value is by Students test. Bars, 8 m.",yes
PMC4227105,Figure_3,oa_package/c6/30/PMC4227105.tar.gz,['Hierarchical Euclidean based clustering of the 2000 most variable loci.'],Figure 3 Samples split into 2 major cluster groups containing either CIMP or CIMP samples (major cluster 2 and 1 respectively). Major cluster 1 split into four sub-clusters. mutated samples are highlighted with a black box. sGBM samples are labeled with their pair number (P#) followed by L to denote late lesion. pGBM samples are labeled with respective TCGA sample names.,yes
PMC11524894,Figure_2,oa_package/e5/a0/PMC11524894.tar.gz,"['89; 2A).', ' 2Validation of multi-class tissue segmentation accuracy and example of WSI processing by the backbone algorithm of platform(A) Segmentation accuracy was tested using an external cohort.', 'The example of WSI processing is presented in 2B.', 'Same color coding as in 2B.', 'Other colors as in 2B.', 'Other colors as in 2B.', '885) using a fully independent test dataset ( 2A) by a significantly more difficult task.']","Figure1 Development of computational platform for non-small cell lung cancer (A) Types of lung cancer specimens and principles of processing in pathology department. Digital pathology allows for diagnostic sign out of cases on the computer monitor and broad application of supportive AI-based tools for automatized slide analysis. (B) Training and external test study cohorts. The slides that were manually annotated and used for development of pixel-wise segmentation algorithms (main algorithm for tissue segmentation and subtyping algorithm) are outlined in Training cohorts as MAIN and SUBTYPE, respectively. UKK L1 SEGM is a manually annotated dataset that was used for formal validation of segmentation accuracy. Further datasets were used for validation of non-small cell lung cancer (NSCLC) subtyping algorithm (at slide level). Abbreviations: MAIN, main dataset for multi-class tissue segmentation algorithm; SUBTYPE, NSCLC subtyping algorithm dataset; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; Magn, magnification; Scanner, Le Leica Aperio; Ham, Hamamatsu Nanozoomer; 3D Hist, 3D Histech; UKK, University Hospital Cologne; AAC, University Hospital Aachen; WNS, Hospital Wiener Neustadt; KAM, Kameda Medical Hospital; ESS, University Hospital Essen; CPTAC, Clinical Proteomic Tumor Analysis Consortium cohort. 107 slide were fully annotated as shown in (D), 61 slide were additionally annotated for underrepresented tissue classes; most of the slides were 40x; some of the slides were scanned under 20x magnification. (C) The main segmentation algorithm was developed using a high-quality large manually annotated dataset. This can be used for quick, primary processing of the whole-slide images and creation of precise, fully quantitative tissue maps. These allow a multiple number of downstream applications, diagnostic (e.g., subtyping) or prognostic/predictive. (D) Principles of high-precision, extensive manual annotations with representative examples of 11 tissue classes are presented. The figure was prepared with BioRender. See also .",yes
PMC8433273,Figure_3,oa_package/3f/30/PMC8433273.tar.gz,"['A CT scan showed almost complete reabsorption of the pelvic hematoma, and a platelet count was 320 103/L under Agrylin treatment ().', 'CT showing resolution of lower pelvic cavity hematoma with intact anastomotic stapling line.', '3DiscussionA systematic literature review was conducted using EMBASE, Medline, and PubMed databases to detect relevant English language articles.']",Fig. 3 CT showing resolution of lower pelvic cavity hematoma with intact anastomotic stapling line.,yes
PMC11510175,Figure_6,oa_package/99/16/PMC11510175.tar.gz,['(a) Nail psoriasis in female at baseline.'],"Figure 6 ( ) Nail psoriasis in female at baseline. PGA-F: 3. ( , ) After 36 weeks of bimekizumab treatment- PGA-F: 0.",yes
PMC4501135,Figure_3,oa_package/7c/84/PMC4501135.tar.gz,"['At the end of surgery, it helps determine if any residual traction exists in the form of secondary or tertiary membranes, as well as in determining the adequacy of membrane peeling [].']",Figure 3 Assessing the adequacy of membrane peeling,yes
PMC8588432,Figure_4,oa_package/4b/c7/PMC8588432.tar.gz,"['Histograms of the fluorescence intensity above the background for V3M2 and V5M2 are shown in A,B, respectively.', 'Dose-dependent binding of selected motifs.']",Figure 4 Dose-dependent binding of selected motifs. Biotin conjugated V3M2 and V5M2 were incubated with vimentin-expressing IGROV cells at various concentrations and followed by streptavidin-FITC staining. Their binding affinity was analyzed by flow cytometry. Histograms presenting the fluorescence intensity above the background were shown for V3M2 ( ) and V5M2 ( ). A scrambled control aptamer with non-specific and low binding affinity is also assessed ( ).,yes
PMC8691626,Figure_4,oa_package/1f/85/PMC8691626.tar.gz,"['g004"" ref-type=""fig"">).', 'Cell lysates from all patients with PLS lacked NSP activity and there were no apparent differences between samples from family A and B ().', 'g004Fluorescence-based protease activity assay for NSP activity of neutrophil lysates.', ', PR3 activity among healthy control neutrophils (C and 4D) is the result of differences in cellular PR3 amounts, different PR3 variants, or levels/activity of endogenous PR3 inhibitors we cannot say, but it is intriguing that a set number of neutrophils from healthy blood donors can display such wide variation in terms of protease activity.', '- In figure 4, why was comparison to PLS3 and 4 not presented for all experiments?', 'C): It seems that CD95 is not increasing much apoptosis (over basal level of death in untreated cells).', '- In figure 4, why was comparison to PLS3 and 4 not presented for all experiments?', 'C): It seems that CD95 is not increasing much apoptosis (over basal level of death in untreated cells).']",10.1371/journal.pone.0261724.g004,yes
PMC9602230,Figure_4,oa_package/f0/05/PMC9602230.tar.gz,['\n\n99mTc-methylene diphosphonate whole-body bone scan.'],"Figure 4 Multiple high radioactive lesions were demonstrated along the right ilium, acetabulum and proximal femur, which were correlated with computed tomography and magnetic resonance imaging findings.",yes
PMC9610451,Figure_1,oa_package/51/ec/PMC9610451.tar.gz,"['Postprocedural imaging of LPS placement was verified with X-ray or computed tomography imaging and graded into four categories: (1) upward placement (correct placement), (2) downward placement, (3) hyperflexion, and (4) subcutaneous migration [].', ':Classification of the spinal catheter position: (a) upward placement (correct placement), (b) downward placement, (c) hyperflexion, and (d) subcutaneous migration.']","Figure 1: Classification of the spinal catheter position: (a) upward placement (correct placement), (b) downward placement, (c) hyperflexion, and (d) subcutaneous migration.",yes
PMC6667521,Figure_9,oa_package/40/96/PMC6667521.tar.gz,"[' 9).', '40-year-old man, incidental finding of a small os supratalare (white arrow), as well as an os trigonum.', 'Patient was referred for radiographs with the suspicion of Achilles tendinopathy in the contralateral legSeveral rare accessory bones in the hindfoot have been described, such as an accessory calcaneus, by Krause and Rouse [30], and bipartite configurations of the talus that can be mistaken by fractures [31 33].']","Fig. 9 40-year-old man, incidental finding of a small os supratalare (white arrow), as well as an os trigonum. Patient was referred for radiographs with the suspicion of Achilles tendinopathy in the contralateral leg",yes
PMC10550728,Figure_3,oa_package/be/1c/PMC10550728.tar.gz,"['', 'In control HepG2 cells, nSMase2 was seen predominantly in the cytoplasm following a punctuated pattern (', 'In PAL-treated cells, the cytosolic fluorescence seemed to decrease and nSMase2 became distinctly visible on the PM (', 'In contrast, OLE treatment had no effect on nSMase2 cellular distribution (', 'This apparent translocation of nSMase2 happened as early as 6 h following PAL treatment (', ' 3B) and was similarly seen in primary mouse hepatocytes (', ' 3C) and in AML-12 cells, a normal, mouse-derived hepatic cell line (', 'Colabeling with the Golgi marker, GM130, showed that in control cells, nSMase2 exhibits some Golgi localization (', 'Costaining for GM130, F-actin, and nSMase2 further confirmed that the effects are indeed caused by translocation of nSMase2 form the Golgi to the PM: In control cells, the predominant site for colocalization (indicated in yellow) was between the GM130 and nSMase2 (upper leftmost panel, ', ' 3I), while in the PAL-treated cells, a colocalization was seen almost only between F-actin and nSMase2 (lower leftmost panel, ', 'Costaining of AML-12 cells confirmed the observations from HepG2 cells, namely that in the PAL-treated cells, nSMase2 localized significantly to the PM (', 'Primary hepatocytes were then isolated and immunolabeled with antibodies against nSMase2 (', ' 3E) and costained with BODIPY, a fluorescent label for lipid droplets (', ' 2\nSupplemental ']","Fig.3 Subcellular localization of nSMase2 in hepatic cell lines or primary hepatocytes following palmitate treatment ex vivo or in hepatocytes from mice with diet-induced obesity. A-F: Palmitate-induced translocation of nSMase2 to the plasma membrane. Various hepatic cell lines and primary hepatocytes were treated with PAL or OLE as indicated. Immunolabeling was done with our custom-made polyclonal anti-nSMase2 antibody (green in A-E, red in F) while nuclei were stained with DAPI (blue). For super resolution microscopy, BODIPI was used to stain lipid droplets (F). A: HepG2 cells treated with PAL or OLE for 18xA0h; (B) HepG2 cells treated with PAL for the indicated times; (C) primary mouse hepatocytes treated with PAL ex vivo for 18xA0h; (D) AML-12xA0cells were treated with PAL for 18xA0h; (E, F) primary hepatocytes isolated from mouse with diet-induced steatosis analyzed by traditional confocal microscopy (E) or super-resolution microscopy (SIM) (F). G-I: Effects of PAL on the colocalization of nSMase2 with Golgi and the plasma membrane markers. HepG2 and AML-12xA0cells were treated with PAL or vehicles as indicated. G: AML-12xA0cells labeled with anti-CD44 antibody (red) or anti-nSMase2 antibody (green) and costained with DAPI (blue). H: HepG2 cells immunolabeled with anti-nSMase2 antibody (green), mouse anti-GM130 antibody (red) and costained with DAPI (blue). I: HepG2 cells immunolabeled with anti-nSMase2 antibody (red), anti-GM130 antibody (purple) and costained for F-actin with Alexa Flour 488 phalloidin (green) and for the nuclei, with DAPI (blue). All pictures shown are representative of multiple independent experiments (n=5 for panel A, 2 for panel B, 2 for panel C, 3 for panel D, 3 for panel F, and 1 for panels G and H). nSMase2, neutral sphingomyelinase 2; PAL, palmitic acid; OLE, oleic acid.",yes
PMC10205740,Figure_2,oa_package/a9/8b/PMC10205740.tar.gz,"['2b we observe that sample noise, though seldom, does occur for segmentation annotations as well.', '2c we showcase a sample where the model gives an almost perfect prediction, with the exception of a small area, where healthy epidermis is predicted right next to the damaged segment.', '2a.', '2a, unlike most samples in the dataset, shows very low contrasts.', '\nQualitative examples from the segmentation model highlighting different aspects: (a) in case of low contrast, the performance drops considerably.', '\nDiscussionIn this work, we evaluate different approaches to estimate cell damage within the epidermis.']","Figure 2 Qualitative examples from the segmentation model highlighting different aspects: ( ) in case of low contrast, the performance drops considerably. ( ) shows robustness to missing annotations (considered as label noise). Figure ( ) shows a case where the metric (macro-average IoU) drops considerably (although qualitatively almost perfect) due to small predictions for absent classes (in this case for healthy epidermis (green) although completely damaged (red)).",yes
PMC5909341,Figure_3,oa_package/1f/e7/PMC5909341.tar.gz,"[' 3).', 'Contrast-enhanced axial CT image one day after TAE illustrating numerous hypoattenuating foci consistent with devascularised metastases\n']",Fig. 3 Contrast-enhanced axial CT image one day after TAE illustrating numerous hypoattenuating foci consistent with devascularised metastases,yes
PMC6773205,Figure_2,oa_package/fa/49/PMC6773205.tar.gz,['A Lack of CD200R1 exacerbates eosinophilia inflammation during fungal infection without altering pathogen clearance.'],"Figure 2 A Lack of CD200R1 exacerbates eosinophilia inflammation during fungal infection without altering pathogen clearance. Wildtype and CD200R1 mice were infected intranasally with . The total cell numbers in the airways (A) and lung tissue (B) were measured at day 7, 14 and 35 post infection. Total alveolar CD11c macrophages in the lung (C) and airways (D) were analysed by flow cytometry. Total eosinophils in the airways and in the lung (E) were analysed by flow cytometry. Data presented as box and whisker plots with median. Open box, wildtype; filled box, CD200R1 (CE). Total CFUs were determined by plating out BAL fluid (F), lung (G), brain (H) and spleen (I) homogenates from wildtype and CD200R1 mice. Open circle, wildtype; Closed triangle, CD200R1 (A, B, FI). Figures present data from 3 independent experiments with 45 mice per group in each experiment. Statistical significance was determined by MannWhitney test, * <0.05; ** <0.01, *** <0.001.",yes
PMC7769104,Figure_1,oa_package/b1/33/PMC7769104.tar.gz,"['Computed tomography (CT) further identified a duplicated odontoid process in association with the previously noted C2 C3 fusion, incomplete anterior arch of C1, variant bony process of the transverse process of C1, and enlarged right jugular foramen ().', '--PMC4813060-->27081234Three-dimensional reconstructed computed tomography of the craniocervical junction in the patient presented herein.']","Fig. 1 Three-dimensional reconstructed computed tomography of the craniocervical junction in the patient presented herein. Note the two ossification centers (arrow heads) of the duplicated odontoid process via a posterior view through the foramen magnum and from a posterior view (left). On this same image, the right jugular foramen (arrow) was significantly enlarged compared to the left. From an anterior view (right), note again the two ossification centers for the apical parts of the odontoid processes (arrow heads), the split anterior arch of C1 (asterisks), and the fused C2 and C3 vertebrae (black arrow). Also, note the right-sided inferior bony extension from the transverse process of C1 (white arrow).",yes
PMC10858775,Figure_4,oa_package/de/ce/PMC10858775.tar.gz,"['The tumor was resected by bifrontal craniotomy [a].', 'Following this, cranioplasty was done using titanium mesh (Johnson and Johnson, India), fixed with miniplate and screw [b].', ':(a) Intraoperative image after skin flap elevation, (b) after resection and placement of mesh over the craniectomy defect.']","Figure 4: (a) Intraoperative image after skin flap elevation, (b) after resection and placement of mesh over the craniectomy defect.",yes
PMC11415918,Figure_1,oa_package/80/1e/PMC11415918.tar.gz,"['Nonfunctional ovaries and regressed ovarian follicles were visible changes in ovaries ().', '.']",Fig. 1. gross lesions from pigeons (3 years old) naturally infected with lymphoid leukosis. A) Liver showing whitish nodules about 12 mm in diameter scattered all over the surface. B) Liver showing petechial hemorrhages all over the surface. C) Spleen showing severe enlargement with large hemorrhagic areas on the surface. D) Kidney shows enlargement and mottling (green arrow). Nonfunctional ovaries and regressed ovarian follicles (black arrow).,yes
PMC7734064,Figure_1,oa_package/91/18/PMC7734064.tar.gz,"['Chest computed tomography (CT) revealed one lesion in the right upper lobe and two lesions in the right middle lobe on June 16, 2019 (A, C and E).', 'Immunohistochemical staining in the post-operative pathology indicated that three nodules of the patient all was clinically diagnosed with stage IA minimally invasive lung adenocarcinoma, T1N0M0 (B, D and F).', 'Chest computed tomography (CT) for the nodules in the lung lobes and the hematoxylin-eosin staining revealed the lung adenocarcinoma cells in the three primary sites (100 m).', '5 cm in diameter) of the right upper lobe (A).', '4 cm in diameter) in the right middle lobe (C).', '6 cm in diameter) in the right middle lobe (E) and confirmed by amplification RNA sequencing with 7574 mutation fragments (C).']","Figure 1 Chest computed tomography (CT) for the nodules in the lung lobes and the hematoxylin-eosin staining revealed the lung adenocarcinoma cells in the three primary sites (100m). Three light red circles show the schematic location of the nodules. ( ) and ( ) for the nidus in the right upper lobe carrying 19del, ( ) and ( ) for the nidus in the right middle lobe carrying 20ins, and ( ) and ( ) for the nidus in the right middle lobe carrying fusion.",yes
PMC9509584,Figure_3,oa_package/6b/f2/PMC9509584.tar.gz,"[' 3).', 'Mechanisms involved in apoE-related neurodegeneration.', ""APOE4 will contribute to additional pathological features characteristic of AD, but also present in other CNS pathologies such as, for instance, impairment of the autophagy/endosomal system, altered microglial reactivity and neuroinflammation, and blood brain barrier disruptionParkinson s diseaseParkinson's Disease (PD) constitutes the second most prevalent neurodegenerative disease.""]","Fig. 3 Mechanisms involved in apoE-related neurodegeneration. The influence of the genotype has been associated with CNS diseases such as Alzheimers Disease (AD), Parkinsons Disease (PD), Traumatic Brain Injury (TBI), Stroke, Amyotrophic Lateral Sclerosis (ALS), and Multiple Sclerosis (MS). In particular, promotes synaptic impairment, amyloid- (A) protein seeding, aggregation, A-plaque formation and defective protein clearance in AD. will contribute to additional pathological features characteristic of AD, but also present in other CNS pathologies such as, for instance, impairment of the autophagy/endosomal system, altered microglial reactivity and neuroinflammation, and bloodbrain barrier disruption",yes
PMC11506826,Figure_9,oa_package/fe/70/PMC11506826.tar.gz,"['Between hyalinized collagen bundles, cells with enlarged hyperchromic and pleomorphic nuclei () were also found.', 'Microscopic aspects of ancient schwannomas HE-stained slides: (a) adjacent to the striated muscle, ob.']","Figure 9 Microscopic aspects of ancient schwannomasHE-stained slides: ( ) adjacent to the striated muscle, ob. 4; ( ) hyalinization areas and hemorrhages, ob. 4; ( ) myxoid areas, ob. 5; ( ) cyst formation, ob. 10; ( ) cyst formation and hemorrhage, ob. 5; and ( ) enlarged hyperchromic and pleomorphic nuclei, ob. 40.",yes
PMC7710042,Figure_9,oa_package/14/31/PMC7710042.tar.gz,"['001, for all sites; ) as well as for the sub-adult/adult killer whales (p 0.', 'g009Blubber thickness in relation to body length.', 'Note: edits discussed below include additions to materials and methods (lines 190-199; 203-207; 223-237), results (lines 478-491; 520-536), description (lines 511-513), ']",10.1371/journal.pone.0242505.g009,yes
PMC3276803,Figure_2,oa_package/3b/eb/PMC3276803.tar.gz,"['Case 2A 79-year-old man presented with an oval shaped, erythematous plaque of 7 cm in diameter on the right buttock area, respectively, following ingestion of a 500 mg tablet of ciprofloxacin ().', 'Case 2.']",Fig. 2 Case 2. Ciprofloxacin-induced fixed drug eruption on lumbar 2-maximal point. Right chronic pyelonephritis (corresponding Head's zones for kidney: Th9-L3).,yes
PMC8040537,Figure_3,oa_package/4a/10/PMC8040537.tar.gz,"['Adem s de cuantificar la extensi n de la afectaci n radiol gica en la primera radiograf a de t rax patol gica de cada paciente, tambi n se han valorado diferentes hallazgos patol gicos, como la presencia de afectaci n alveolar y la de opacidades lineales (especialmente la presencia de atelectasias laminares; fig. 3\n).']",Figura 3 Mujer de 52 aos con infeccin por SARS-CoV-2. Detalle de la radiografa de trax posteroanterior que muestra dos opacidades lineales perifricas (flechas).,yes
PMC6929054,Figure_4,oa_package/a6/3f/PMC6929054.tar.gz,"['To determine if protection\nwas antibody-mediated and to minimize\npotential nonspecific effects by the vaccination, we performed challenge\nexperiments following serum transfer and following active vaccination\n4 weeks, instead of 2 weeks, after the third immunization (A).', 'Mice that received 13 mounted significant IgG responses to 2, whereas\ncontrol mice immunized with CRM197 did not (B).', 'As expected, mice immunized\nwith 13 were significantly protected against colonization\nby VPI 10463 (C).', 'difficile, compared to\nmice that received anti-CRM197 serum (D).', 'Therefore, we repeated transfer experiments using pooled sera obtained\nfrom mice immunized with CRM197 or 13 (E).', 'Six of 10 mice that received 13 mounted an IgG response against 2 detectable\nin serum diluted 1:100 (F).', 'Pooled sera of mice immunized with 13 significantly protected\nmice from colonization by VPI 10463 after serum transfer (G).', 'Challenge experiments\nin mice following serum transfer.']","Figure 4 Challenge experimentsin mice following serum transfer. (A) Mice( = 8 per group) were immunized with CRM or three times. (B) 25 days after the third vaccination,sera were subjected to microarray IgG analysis. (C) 28 days afterthe third vaccination, mice were challenged with VPI 10463 and intestinalcolonization was analyzed. (D) Pooled sera from the same mice wereused for transfer experiments into 3 recipient mice per group. (E)Mice ( = 10 per group) were immunized with CRM , , or PBS as control. (F) Immune sera obtained21 days after the third vaccination were subjected to microarray IgGanalysis. (G) Pooled sera from the same mice and mAbs were used fortransfer experiments into 68 recipient mice per group. Notethat - serum was pooled only from 9 of 10 micewith a signal against by microarray (see panel F thatwas performed at 1:100 serum dilution; three additional mice showeda signal at 1:10 serum dilution). Bars in all panels show median +interquartile range. Significance in panels (B), (C), and (F) wasinferred by two-tailed MannWhitney tests[(*) 0.05; n.s. = not significant ( > 0.05)]. Significance in panel (G) was inferred byDunn-correctedKruskalWallis tests [(*) 0.05;(**) 0.01].",yes
PMC6711657,Figure_1,oa_package/32/8f/PMC6711657.tar.gz,"[""ResultsAntigen characterizationAll patients' specimens yielded the same synaptic immunofluorescence pattern of IgG binding, with prominent staining of the basal ganglia and related nuclei, to a lesser extent the hippocampus, and faint staining of the cerebellar granular layer (figure 1, A C)."", 'Detection of the patients with phosphodiesterase 10A autoantibodyIndirect immunofluorescence assay performed on murine tissue with patient serum demonstrates synaptic staining of the basal ganglia (A), more prominent than the hippocampus (B) and to a lesser extent the granular layer of the cerebellum (C).', ""WB of patients' sera using pig basal ganglia extracts revealed a common band (approximate MW 75 kDa) in 3 patients (figure 1D)."", ""IgG eluted from the nitrocellulose-corresponding band demonstrated the same staining pattern by tissue IFA as the patients' serum (figure 1E), but not IgG eluted from a control band ( 150 kDa)."", ""The 2 patients whose serum lacked 75 kDa reactivity in WB testing with pig basal ganglia extracts (figure 1D) were positive by recombinant WB but yielded a less intense signal than the other patients' sera."", 'Both patients had hyperkinetic movement disorders and basal ganglial fluid-attenuated inversion recovery (FLAIR)/T2 hyperintensities (nonenhancing) on MRI that recapitulated the appearance of patient IgG staining observed on mouse brain by IFA (figure 1F).']","Figure 1 Detection of the patients with phosphodiesterase 10A autoantibody Indirect immunofluorescence assay performed on murine tissue with patient serum demonstrates synaptic staining of the basal ganglia (A), more prominent than the hippocampus (B) and to a lesser extent the granular layer of the cerebellum (C). (D) Western blot probing of lysed membrane fraction of pig basal ganglia with serum immunoglobulin G (IgG) of 3 patients identified an immunoreactive band 75 kDa and another >200 kDa that were not seen with normal control serum IgG. Elution of patient 1's IgG from the corresponding 75 kDa excised nitrocellulose band reproduced the original tissue staining pattern when applied to mouse brain as the patient's serum (E), while an IgG elution from a control band (150 kDa) did not (not shown). (F) T2 fluid-attenuated inversion recovery MRI of patient 4 with bilateral basal ganglial hyperintensities. GL = granular layer; GP = globus pallidus; Hi = hippocampus; ML = molecular layer; SN = substantia nigra; Str = striatum.",yes
PMC6452215,Figure_2,oa_package/da/ca/PMC6452215.tar.gz,[],"FIGURE 2 Representative images for linear type of monoclonal TBMID. This case (Patient 9) showed that linear IgG2 and light chain depositions in TBM (yellow arrow). IgG1, IgG3, IgG4, IgA, IgM and light chain were negative in TBM.",yes
PMC8260203,Figure_3,oa_package/6c/21/PMC8260203.tar.gz,[],Figure 3 Picture taken from left main angiography showing patent coronary arteries and prominent Thebesian veins as demonstrated by the red arrows,yes
PMC7763900,Figure_2,oa_package/31/00/PMC7763900.tar.gz,"['PN21Absolute heart mass (A) of the PGR group (males: 0.', 'When heart mass was standardized to body surface area (B), no significant (p = 0.', '63 mm, C) when compared to the CON group (males: 15.', '991) differences in absolute heart mass (A) between the PGR group (male: 0.', 'When hearts were standardized to body surface area (B), there were no significant (p = 0.', '001, C) than the CON group (male: 18.', 'Absolute heart mass of PGR mice was significantly less than controls at PN21, but not at PN80, suggesting heart mass was recuperated even though body mass was reduced ().', 'Effects of PGR on heart mass and tibia length: (A) absolute heart mass (PN21/PN80): The PN21-PGR group (n = 6) was significantly reduced compared to CON (n = 6); (*: p = 0.']","Figure 2 Effects of PGR on heart mass and tibia length: ( ) absolute heart mass (PN21/PN80): The PN21-PGR group ( = 6) was significantly reduced compared to CON ( = 6); (*: = 0.01). PGR-F were significantly less than CON-M (#: = 0.05) but not less than CON-F ( = 0.09). PN80-PGR was not significantly ( > 0.05) different from the CON group, and no sex effect was present. ( ) Standardized heart mass (PN21/PN80): no significant differences ( < 0.05). ( ) Tibia length (PN21/PN80): PGR significantly reduced average tibia length compared to the CON group at PN21 (CON: = 6, PGR: = 6) and PN80 (CON: = 12, PGR: = 7); (*: < 0.001), with no sex effect present at PN21. At PN80, a diet x sex interaction (#: = 0.04) revealed the tibias of PGR-M and -F were smaller than CON-M but not CON-F (^: < 0.01). Values are mean SD.",yes
PMC8576671,Figure_2,oa_package/ee/47/PMC8576671.tar.gz,"['But for small nodes beyond PET resolution and hard to observe CT sign, missed diagnosis is common in both criteria ().', 'A malignant lymph node of Oesophageal squamous cell carcinoma.']","Figure 2 A malignant lymph node of Oesophageal squamous cell carcinoma. Images obtained with PET/CT showed a small lymph node in Group 2L (arrow), 0.3 cm in diameter, too small to reach the revolution of PET and hard to observe CT sign, no increased FDG uptake, both criteria missed diagnosis. Histological section showed a metastatic squamous cell carcinoma (haematoxylin-eosin stain; original magnification, 100).",yes
PMC10667534,Figure_1,oa_package/43/be/PMC10667534.tar.gz,"[' 1a and b.', ' 1a) nor HIF-2 (', ' 1b) protein in 2-week Roxa-treated mice was significantly different from control mice (p = 0.', ' 1c).', 'Verification that Roxadustat stabilizes HIF1- in the retina.', 'Roxa increases glycolysis and reduces glycolytic reserveTo confirm an impact of Roxadustat on glycolytic cellular metabolism, we cultured primary M ller glia, treated the cells with Roxadustat for 48 h, then used the Seahorse Bioanalyzer to examine glycolysis.']","Figure 1 Verification that Roxadustat stabilizes HIF1- in the retina. ( , ) Protein analysis of retinal lysates shows that I.P. injection of Roxadustat increased HIF-1 and HIF-2 protein in the retina. HIF-1 was significantly higher (F2,9=80, ****p<0.0001) in 4-week Roxa retinas compared to 2-week Roxa and control retinas. HIF-2 was significantly higher (F2,9=77.23, ****p<0.0001) in 4-week Roxa retinas compared to 2-week Roxa and control retinas. Tukey's multiple comparisons test showed a significant increase in HIF-1 and HIF-2 after 4weeks of Roxa treatment (****p<0.0001) compared to 2-week Roxa (****p<0.0001) and control (****p<0.0001) retinas. There was no difference in HIF-1 and HIF-2 in 2-week Roxa and control retinas (p=0.8989, p=0.7366, respectively). Values shown are meanSEM of per group. C) Retinal cross-section from CAG-CreERT2-ODD x ROSA26 floxd STOP tdTomato mice 3days after injection with Roxa and no OHT showing tdTomato (tdT, red) expression in cells with stabilized HIF-1. RGCs (green) and Mller glia (blue, right panel) indicate that the primary tdTomato immunolabel is in Mller glial cell somata (arrowhead), and Mller glial basal processes (arrow). The right panel of (c) is the same view as the left, though showing Vimentin immunolabel in lieu of DAPI. GCL: ganglion cell layer; INL: inner nuclear layer. Scale bar: 25m.",yes
PMC1872031,Figure_7,oa_package/14/be/PMC1872031.tar.gz,['1.'],"Figure 7 1. Smear showing myeloma cells. [MGG; 400]. 2. Histopathology section showing dispersed tumor cells many having a ""clock-face"" condensation of chromatin. [H&E 200].",yes
PMC6039179,Figure_12,oa_package/79/4a/PMC6039179.tar.gz,[],10.7554/eLife.35449.014,yes
PMC9391516,Figure_4,oa_package/99/49/PMC9391516.tar.gz,"['Six hemi-sections along the rostral-caudal axis were analyzed ( 4).', ' 4Quantification of vector genome copies following intracisternal injection of multiple AAV serotypesqPCR was performed to assess distribution of vector genome copies in the brain.']",Figure3 Transduction of other brain structures by multiple AAV serotypes following intracisternal injection Normal 4-week-old cats (n= 2 for AAV9; n= 3 for all other vectors) received injections of 110 vg vector encoding EGFP. GFP-expressing cells in specific structures within the brain were counted following DAB immunohistochemistry for EGFP at 4weeks post-injection. Values are expressed as number of GFP-expressing cells per square millimeter. Bars represent means+ SEMs.,yes
PMC9434002,Figure_6,oa_package/fe/f6/PMC9434002.tar.gz,['BREAST lumpectomy block diagnosed with focus of invasive carcinoma.'],"Fig. 6 BREAST lumpectomy block diagnosed with focus of invasive carcinoma. (a)Photograph of specimen. (b)Average projection along -axis. (c)H&E histology slide of block. (d)OCT representation of nodule containing reactive stroma with neovascularity and mild chronic inflammation (left) and well-differentiated IDC with reactive stroma (right). Insets show that reactive stroma is more heterogenous and lowly reflective in OCT compared with bright, homogenous cancer. (e)Corresponding H&E histology. (f)OCT B-scan through nodule showing that stroma shows heterogeneous texture and deeper depth penetration (left) compared with highly reflective and lower depth penetration cancer (right). (a)(c) ; (d)(f) .",yes
PMC10470673,Figure_3,oa_package/3c/c6/PMC10470673.tar.gz,['.'],"Figure 3. Brain MRI imaging before and after treatment in case 3. The lentiform nucleus is symmetrically swollen on both sides with long T1 and T2 signals, which indicate LFS characteristics. (A) T1 image of the brain shows symmetrical low-intensity signals in bilateral basal ganglia (arrow), while (B) symmetrical high signal intensity in bilateral basal ganglia can be seen on T2 images of the brain (arrow) before LFS treatment. No abnormal signal was found on (C) T1 (arrow) and (D) T2 (arrow) signals after gamma globulins pulse therapy. LFS = lentiform fork sign, MRI = magnetic resonance imaging.",yes
PMC3032862,Figure_5,oa_package/b2/bc/PMC3032862.tar.gz,"[' 5).', '', '\nThe fracture is highly specific for child abuse in infancy, ie, when a patient is nonambulatory, usually in the first year of life.']",Fig.5 An AP radiograph of the knee of a 4-month-old infant shows a CML at the lateral aspect of the distal femoral metaphysis (arrowhead). Note periosteal reaction (curved arrow) seen more proximally and ending at the level of the physis. Also note a healing corner fracture of the proximal tibia (arrow).,yes
PMC9655885,Figure_3,oa_package/18/b1/PMC9655885.tar.gz,"['Necrotic death occurred in 50% of the cell population (A,B, red columns), whereas in the control without glutamate exposure, cellular death was recorded in no more than 8 12% of the cells.', 'However, a significant redistribution regarding the death pathways was observed due to a decrease in the rate of late stages of apoptosis (21%) and an increase in the rate of the early stages (23%) without a significant decrease in necrotic death (A, blue symbols).', 'The culture preincubation with 100 M 3-EA increased the percentage of viable cells to 29% and reduced the number of cells in the late stages of apoptosis (16%) and the percentage of necrotic death (18%) (A, green symbols).', 'Ischemia-like conditions for 40 min in vitro caused a biphasic increase in [Ca2+]i in neurons (A) and astrocytes (B) of the cerebral cortex, which correlated with necrotic death cells detected by the appearance of propidium iodide fluorescence in the cellular nuclei (C, OGD).', 'An effect of 24 h preincubation of the cortex cells with various concentrations of 3-EA on the viability of the cells during 24 h glutamate excitotoxicity (GluTox): (A) Cytogram showing the viability of cerebral cortex cells (horizontal axis propidium iodide fluorescence intensity; vertical axis Hoechst 33342 fluorescence intensity).']","Figure 2 An effect of 24 h preincubation of cortical cells with various concentrations of 3-EA on the increase in [Ca2+]i upon induction of glutamate excitotoxicity for 50 min (GluTox, 100 M glutamate + 20 M glycine in Mg -free medium). ( , )averaged Ca signals of neurons ( ) and astrocytes ( ) of the cerebral cortex during the induction of glutamate excitotoxicity. Short-term (30 s) applications of KCl (35 mM) and ATP (10 M) were performed to detect neurons and astrocytes, respectively. The experiments were performed in triplicate on three cell cultures of different passages.",yes
PMC8042417,Figure_3,oa_package/c1/d7/PMC8042417.tar.gz,"['After coil embolization, occlusion of the pathological vessels was achieved ().', '.']",Figure 3. Catheter angiogram of the splenic artery via the coeliac axis shows a distal pseudo-aneurysm marked in ( ) by white arrow. ( ) Catheter angiogram post-coil embolization: the pseudo-aneurysm was successfully occluded.,yes
PMC7509431,Figure_5,oa_package/eb/24/PMC7509431.tar.gz,[],"FIGURE 5 Prickle2 treatment reduces Tau phosphorylation in 3 Tg mice. Representative IHC images of WT, 3 Tg and 3 Tg/Prickle2 treatment mice stained with anti-Tau (phospho S202) antibodies. The proportions of pS202-positive cells were calculated in cortical and hippocampal, respectively ( = 6 per group). Representative IHC images of WT, 3 Tg and 3 Tg/Prickle2 treatment mice stained with anti-Tau (phospho S396) antibodies. The proportions of pS396-positive cells were calculated in cortical and hippocampal, respectively ( = 6 per group). Western blots detected the phosphorylated Tau using pS202 and pS396 antibodies in the brain tissues from WT, 3 Tg and 3 Tg/Prickle2 mice. Quantification of the protein levels of phosphorylated Tau by densitometric analyses ( = 6 per group). ANOVA followed by Bonferronis post hoc test. < 0.01; < 0.001; < 0.0001.",yes
PMC6098145,Figure_1,oa_package/43/5c/PMC6098145.tar.gz,"[' 1A C).', '1/166/151/164/1619%Klebsiella oxytoca is increased in tumor-bearing mice with cachexia independently of anorexia.', 'Undernutrition and malnutrition have been shown to be associated with microbial dysbiosis20.', ' 1D).']","Figure 1 is increased in tumor-bearing mice with cachexia independently of anorexia. ( ) Cecal . levels in three independent cohorts of sham-injected mice (CT) and C26-injected mice (C26), n=78 for each group of mice. ( ) Cecal . levels in sham-injected mice (CT), C26-injected mice (C26), healthy mice pair-fed to sham-injected mice (PFtoCT) and healthy mice pair-fed to C26-injected mice (PFtoC26), n=78. Data are presented as whiskers plots with minimal and maximal values. *p<0.05; **p<0.01; ***p<0.001.",yes
PMC4912437,Figure_5,oa_package/1b/2d/PMC4912437.tar.gz,"['To accomplish this, we set out to modify standard motor neuron differentiation protocols (schematic in 5A).', 'When analyzed by immunocytochemistry at day 20 of differentiation, cells in spheres expressed ISL1, MAP2, ChAT, and SYNAPSIN1 (s 5B, S6A, and S6B).', 'Immunocytochemistry revealed that up to 85% of the dissociated cells expressed ISL1, with consistent results obtained in different cell lines and low experiment-to-experiment variability (s 5C and 5D; Table S3).', 'We also found that the vast majority of cells in the cultures were immunopositive for ChAT (s 5E and 5F; Table S3).', ' 53D Neuronal Spheres from Human PSC Lines Differentiate into Motor Neurons in the Presence of Patterning Factors(A) Schematic illustration of differentiation strategy used to obtain motor neurons from 3D spheres.']","Figure5 3D Neuronal Spheres from Human PSC Lines Differentiate into Motor Neurons in the Presence of Patterning Factors (A) Schematic illustration of differentiation strategy used to obtain motor neurons from 3D spheres. CNTF, ciliary neurotrophic factor. (B) Representative images of immunohistochemistry on sections of 1016A-derived spheres differentiated towards the motor neuron fate and stained with antibodies specific for MAP2 (red) and ISL1 (green) at day 20 of differentiation. Nuclei were counterstained with DAPI (blue). Scale bar represents 50m. (C) Representative images of immunocytochemistry of 1016A-derived motor neurons from dissociated spheres plated and cultured for 7days after dissociation. Cells were stained using antibodies specific for MAP2 (purple) and ISL1 (green). Nuclei were counterstained with DAPI (blue). Scale bar represents 50m. (D) Quantification of the relative proportion of the different motor neurons subtypes obtained from experiments in different hPSC lines. Cells were dissociated and plated at day 15, cultured for 1week, and processed for immunocytochemistry with an antibody specific for ISL1. Error bars indicate SD from three independent experiments. (E) Representative immunocytochemistry of 1016A-derived neurons from spheres stained with antibodies specific for MAP2 (purple), ISL1 (green), and ChAT (red). Nuclei were counterstained with DAPI. Scale bar represents 50m. (F) Quantification of the relative proportion of ChAT-positive cells obtained from experiments in two different hPSC lines. Cells were dissociated and plated at day 15, cultured for 1week, and processed for immunocytochemistry with an antibody specific for ChAT. Error bars indicate SD from three independent experiments. See also ; ; .",yes
PMC9066474,Figure_2,oa_package/4f/35/PMC9066474.tar.gz,"['After excision of the metal struts, the stented region (figure 2B), with reference segments (figure 2A, C) within 5 mm of both the proximal and distal ends, was processed for H E staining or immunohistochemical staining.', 'Left panel: The extracted proximal LCX segment with a stent.', 'Light microscopy of cross-sections of the artery, stained by H E, revealed eccentric arteriosclerotic plaques in reference segments (figure 2 A# C#) and stented segment (figure 2 B#).', 'On higher magnification, the stented segment appeared hypocellular, with scant inflammatory cells including lymphocytes or neutrophils observed around the stent struts (figure 2D, E).']","Figure 2 Left panel: The extracted proximal LCX segment with a stent. Blue lines A, B and C correspond to the sites of cross-sectioning of the artery. Middle panel: Cross-sectional images of the proximal LCX (A#), stented segment (B#) and distal LCX (C#) stained for H&E. Right panel: D, E represent high magnification H&E-stained images of the stented segment around the stent struts. LCX: left circumflex.",yes
PMC4127522,Figure_1,oa_package/64/96/PMC4127522.tar.gz,['Abdominal computer tomography showing the lesion in the head of the pancreas.'],Figure 1 Abdominal computer tomography showing the lesion in the head of the pancreas.,yes
PMC2092377,Figure_3,oa_package/2b/e4/PMC2092377.tar.gz,"['g002""/>Congenital LCMV Infection of Humans Induces Periventricular CalcificationsThe neonatal rat model demonstrates that periventricular neurons are selectively vulnerable to infection with LCMV.', 'In the cerebellar ventral lobules, LCMV induces a neuronal migration disturbance, which causes cerebellar granule cells to be permanently ectopically located within the molecular layer ().']","Figure 3 Congenital LCMV Infection of Humans Induces Periventricular Calcifications The neonatal rat model demonstrates that periventricular neurons are selectively vulnerable to infection with LCMV. (A) Head CT scan from an infant with congenital LCMV infection. The scan reveals microencephaly and prominent periventricular calcifications (arrows). In addition, this scan reveals an abnormal cortical gyral pattern (arrowheads), suggestive of disturbed cortical neuronal migration. (B) Horizontal section (50-m-thick) through a 49-d-old rat brain immunohistochemically stained for LCMV. The rat was inoculated as a neonate with LCMV. Infection is localized to the periventricular region (arrows). L = lateral ventricle, S = septum, BG = basal ganglia. (C) Higher magnification of the boxed area in (B) shows that the infected cells are neuronal in morphology. Viral antigen is present in neuronal cell bodies (arrows) and neurites (arrowheads). Magnification bars represent 500 um in (B) and 100 um in (C).",yes
PMC3925676,Figure_6,oa_package/41/5e/PMC3925676.tar.gz,"['We were unable to detect K1 or K10 expression in the NT pools which has been adapted in low Ca2+ media, with the expression of these two proteins being detected only when the cells were grown in high Ca2+ media that induces differentiation (c and d).', 'Growth of NT HaCaT cells in the high Ca2+ media also increased the expression level of matriptase itself as well as involucrin (a and e), while having minimal impact, if any, on the expression level of HAI-1.', 'After exposing the cells in high calcium media for one day, the K1 and K10 expression levels were significantly lower in the MTP KD HaCaT pools as compared to the NT control pools (c and d).', 'Similarly, the 24 hour-exposure to high Ca2+ media failed to increase the expression level of involucrin in the MTP KD pools (e).', 'As observed for the NT HaCaT pools, higher matriptase expression was observed in MTP KD clones grown in high Ca2+ media versus low Ca2+ media (a), while the expression of HAI-1 was not affected by the calcium concentration of the growth media (', 'This phenomenon can be seen not only for the NT cells but also for MTP KD cells (f, g, and h).', 'Role of matriptase in keratinocyte differentiationThe mRNA levels of matriptase, HAI-1, K1, K10, and involucrin were analyzed by real-time PCR in matriptase knockdown pools 52 (MTP KD) and the non-target control (NT).']","Figure 6 Role of matriptase in keratinocyte differentiation The mRNA levels of matriptase, HAI-1, K1, K10, and involucrin were analyzed by real-time PCR in matriptase knockdown pools 52 (MTP KD) and the non-target control (NT). The cells were grown either in low Ca or high Ca media for 24 hr (panels ae), as indicated. In the panels f, g, and h, the cells were grown in low Ca for 1 day or 5 days, as indicated. The fold changes of mRNA levels were quantified by normalization relative to the controls indicated. Mean values SD (n=4) are plotted. The results presented are from one experiment representative of the four performed.",yes
PMC6243870,Figure_1,oa_package/a6/da/PMC6243870.tar.gz,"['Fibrous connective tissue hyperplasia and residual gelatin sponge were observed (A).', 'Large necrotic areas and gelatin sponge residue were observed (B).', 'Source of support: This study was supported by the Research Fund Youth Scientific and Technological Creative Team from the Sichuan Office of Technology (2016TD0008)References1NiezgodaJASerenaTECarterMJOutcomes of radiation injuries using hyperbaric oxygen therapy: An observational cohort studyAdv Skin Wound Care2016291219266500922BeumerJHarrisonRSandersBKurraschMOsteoradionecrosis: Predisposing factors and outcomes of therapyHead Neck Surg198468192767066233SchoenPJRaghoebarGMBoumaJRehabilitation of oral function in head and neck cancer patients after radiotherapy with implant-retained dentures: Effects of hyperbaric oxygen therapyOral Oncol20074337988169967834OguzEEkinciSErogluMEvaluation and comparison of the effects of hyperbaric oxygen and ozonized oxygen as adjuvant treatments in an experimental osteomyelitis modelJ Surg Res2011171e6168219205515NolenDCannadySBWaxMKComparison of complications in free flap reconstruction for osteoradionecrosis in patients with or without hyperbaric oxygen therapyHead Neck20143617014241236576EspositoMGrusovinMGPatelSInterventions for replacing missing teeth: Hyperbaric oxygen therapy for irradiated patients who require dental implantsCochrane Database Syst Rev200817123347WangZShiBLuDSongQA histological study on healing process of palatal wound with denuded bone restored with transplanted buccal or palatal mucosaWest China J Stomatol200220326288YangFHuDBaiXEffects of vacuum sealing drainage on chemotaxis of inflammatory cells and secretion of inflammatory cytokines in skin wound of rabbitsChongqing Med201241686889FreedmanJEVitsevaOTanriverdiKThe role of the blood transcriptome in innate inflammation and strokeAnn NY Acad Sci20121207414510InceBArslanADadaciMThe effect of different application timings of hyperbaric oxygen treatment on nerve regeneration in ratsMicrosurgery201636586922677327611WangWJinLWangGEffect of hyperbaric oxygen on survival rate of rats after resuscitation from traumatic shockChin J Naut Med Hyperb Med200815192412MarxREAmesJRThe use of hyperbaric oxygen therapy in bony reconstruction of the irradiated and tissue-deficient patientJ Oral Maxillofac Surg19824041220704530313KangTSGortiGKQuanSYEffect of hyperbaric oxygen on the growth factor profile of fibroblastsArch Facial Plast Surg2004631351473264214ValarmathiMTDavisJMYostMJA three-dimensional model of vasculogenesisBiomaterials20093010981121902715415KasuyaATokuraYAttempts to accelerate wound healingJ Dermatol Sci201476169722546835716LancerottoLOrgillDPMechanoregulation of angiogenesis in wound healingAdv Wound Care201436263417BilicIPetriNMBezicJEffects of hyperbaric oxygen therapy on experimental burn wound healing in rats: A randomized controlled studyUndersea Hyperb Med200532191579630918SheikhAYRollinsMDHopfHWHuntTKHyperoxia improves microvascular perfusion in a murine wound modelWound Repair Regen20051330381595305019LinSShyuKGLeeCCHyperbaric oxygen selectively induces angiopoietin-2 in human umbilical vein endothelial cellsBiochem Biophys Res Commun2002296710151217604020SanoHIchiokaSSekiyaNInfluence of oxygen on wound healing dynamics: Assessment in a novel wound mouse model under a variable oxygen environmentPLoS One20127e5021223209678Images of tissue sections stained with hematoxylin-eosin from the HBO group (A) and control group (B) on postoperative day 3.']",Figure 1 Images of tissue sections stained with hematoxylin-eosin from the HBO group ( ) and control group ( ) on postoperative day 3. Image at 400 magnification.,yes
PMC9047774,Figure_1,oa_package/52/c2/PMC9047774.tar.gz,"['His\ninitial chest radiograph (CXR) showed extensive bilateral pulmonary parenchymal disease\n().', '.', '1177_23247096221095426-fig1"" position=""float""/>Computed tomography (CT) of the chest performed showed patchy, confluent, extensive\nbilateral consolidative, and interstitial ground-glass opacities, increased from the prior\nCXR.', 'Her initial CXR demonstrated patchy, bilateral airspace disease ().', 'His CXR showed patchy parenchymal airspace disease ().', 'Initial CXR showed patchy airspace disease more pronounced at the\nbilateral periphery and bases (\n1).', 'Initial CXR showed patchy airspace disease involving bilateral\nbases ().', 'His CXR revealed moderate to\nsevere diffuse bilateral patchy pulmonary opacities, left greater than right ().', '9 cm in the right lower lung\nfield, without any other parenchymal changes ().', 'His initial CXR showed moderate to large regions of ground-glass\nopacification with increased thickening of the interlobular septa in the periphery ().', 'His initial CXR showed\nbilateral basilar and peripheral infiltrates, without cystic disease ().']","Figure 1. Initial presentation chest radiographs portraying diffuse bilateral opacities,compatible with COVID-19. Number on image corresponds, respectively, to patient case. Abbreviation: COVID-19, coronavirus disease 2019.",yes
PMC8397610,Figure_1,oa_package/8a/af/PMC8397610.tar.gz,"['1.', 'GMV in TN patients compared to HC subjects evaluated by MRI.', 'Images show reduced GMV in TN1 (top 2 rows), TN2 (middle 2 rows), and TN3 (bottom 2 rows) compared to HCsComparisons of altered brain regions between TN groups and HCs.']","Fig. 1 GMV in TN patients compared to HC subjects evaluated by MRI. Images show reduced GMV in TN1 (top 2 rows), TN2 (middle 2 rows), and TN3 (bottom 2 rows) compared to HCs",yes
PMC9497849,Figure_5,oa_package/2c/a7/PMC9497849.tar.gz,"['2% of cases (18/23) (Nioi-Napoli sign I Stade- for more details see ).', 'Nioi Napoli sign.']","Figure 5 NioiNapoli sign. The NioiNapoli sign is a morphological feature occurring in the posterior part of the cornea in the postmortem period. This sign is likely due to two different phenomena that take place in distant portions of the cornea: the dehydration of the anterior tissues and the edema of the posterior part (which has repercussions in all contiguous tissue of the stroma, epithelium included. From the morphological point of view, it consists of different phases or stages: ( ) Stade I: initial posterior waving. ( ) Stade II posterior waving with 1 peak. ( ) Stade III posterior waving with multiple peaks and initial stromal swelling. ( ) Stade IV posterior waving and multiple peaks with extreme stomal swelling.",yes
PMC4451739,Figure_5,oa_package/03/e7/PMC4451739.tar.gz,['Platforms for complement activation.'],"Figure 5 . Properdin is released from activated neutrophils and is bound to the cell membrane where it recruits C3b to form the alternative pathway C3 convertases. C5a then activates additionally neutrophils and they secrete more properdin. This installs a vicious cycle of neutrophil and complement activation. Properdin released from neutrophils or in the plasma binds to activated platelets promoting C3(H O) recruitment and complement activation. Stimulation of endothelial cells with C3a, heme, or other agonists induces expression of P-selectin. P-selectin contains CCP domains and binds C3b, promoting formation of C3 convertases that generate more C3a to stimulate cells.",yes
PMC11128249,Figure_4,oa_package/64/2f/PMC11128249.tar.gz,['Right anterior oblique (RAO) view of left heart catheterization (LHC) with no significant left coronary artery diseaseLeft anterior oblique (LAO) view of the left heart catheterization (LHC) with no significant right coronary artery diseaseOur patient was placed on cardiopulmonary bypass and underwent sternotomy with successful resection of the right atrial singular circular mass.'],Figure 4 Right anterior oblique (RAO) view of left heart catheterization (LHC) with no significant left coronary artery disease,yes
PMC7779532,Figure_1,oa_package/7a/e8/PMC7779532.tar.gz,"['At birth, she had absence of skin on the anteromedial aspect of the lower portion of the left leg extending down through the dorsum of the foot and toes ().', 'Skin findings during infancy.']",Fig 1 Skin findings during infancy. Absence of skin along the left lower extremity at birth.,yes
PMC4628268,Figure_7,oa_package/66/a0/PMC4628268.tar.gz,"[' 7a d, both AACOCF3 and pyrrophenone dose-dependently inhibited LPS- or IFN -induced ROS production where measured at 12 h post-stimulation.', ' 7e, f).', 'ROS production in BV-2 cells after LPS or IFN stimulation was inhibited by cPLA2 pharmacological inhibitors or siRNA knockdown.', '001cPLA2 inhibition prevents morphological changes associated with activation in primary microgliaIn order to visualize the morphological changes of microglia under activation by LPS or IFN , primary microglia were cultured in coverslips, followed by fixation and immunostaining with Iba-1 (red), marker for microglial cells and phalloidin (green) for actin filaments.']","Fig. 7 ROS production in BV-2 cells after LPS or IFN stimulation was inhibited by cPLA pharmacological inhibitors or siRNA knockdown. BV-2 cells were starved for 4h in serum-free DMEM. One hour prior to stimulation, cells were pretreated with the indicated concentrations of cPLA inhibitors: , AACOCF3 or , pyrrophenone. Cells were then stimulated with , 200ng/mL LPS or , 20ng/mL IFN. Alternatively, BV-2 cells were transfected with siRNA against cPLA 24h before being stimulated with LPS or IFN. For all experiments, ROS production was measured 12h post-stimulation by CM-H2DCFDA fluorescence as described in the text. Results were expressed as the meanSEM ( =3) and significant difference between the respective groups was determined by one-way ANOVA followed by Dunnetts post-tests, ** <0.01, *** <0.001",yes
PMC6939189,Figure_3,oa_package/9a/50/PMC6939189.tar.gz,['Details of the lesions.'],Figure 3 Details of the lesions.,yes
PMC5102912,Figure_2,oa_package/5f/5d/PMC5102912.tar.gz,"['HER2 = human epithelial growth factor receptor 2Triple negative breast cancer (Ki67 = 60%, HER2 negative) presenting as mass with circumscribed margin on mammogram.']","Fig. 2 Triple negative breast cancer (Ki67 = 60%, HER2 negative) presenting as mass with circumscribed margin on mammogram. HER2 = human epithelial growth factor receptor 2",yes
PMC4641346,Figure_5,oa_package/90/5d/PMC4641346.tar.gz,['view (upright standing) throughout the 3 year follow-upPre- and postoperative conventional X-rays in the lateral view (upright standing) throughout the 3 year follow-up.'],Fig. 5 Pre- and post-operative conventional X-rays in the a.p. view ( ) throughout the 3year follow-up,yes
PMC7431516,Figure_3,oa_package/f4/ae/PMC7431516.tar.gz,"['3).', 'A 57-year-old male with pain in the medial malleolus without a history of trauma.', 'There is a minor irregularity of the cartilage signal in the subchondral central part of the talar trochlea, probably after previous injuriesThe distraction of bone, which is visible in an avulsion injury, causes a linear trabecular disruption in a limited area (', '33).', '3A 7-year-old girl with chronic pain in both feet.', 'MRI shows a developmental variation in the bone marrow signal in the calcaneus (arrows)In the region of the sinus tarsi in the calcaneus, a cystic structure is often observed which is a vascular remnant (', '34).', '4A 30-year old patient after ankle joint sprain.', '(2) BME was seen at the level of the Lisfranc jointIn the ankle region, MR artifacts are typically located at the level of the lateral malleolus and may imitate BME.', '35).', '5An 18-year-old runner with Achilles tendon pain.', 'There were no changes in the Achilles tendonConclusionThe distribution of BME seen in specific types of injury represents one of the most useful differential diagnostic clues in ankle MRI.']","Fig. 3 A 57-year-old male with pain in the medial malleolus without a history of trauma. Radiograph, mortise view, PD FS coronal image, and T1-weighted coronal image. MRI showed an occult fracture of the medial malleolus (arrow) which in retrospect was visible on the radiographs. There is a minor irregularity of the cartilage signal in the subchondral central part of the talar trochlea, probably after previous injuries",yes
PMC10520382,Figure_20,oa_package/39/3a/PMC10520382.tar.gz,[],"Figure 20 Inflammatory infiltrate also contained a small number of CD20-positive B-cells. Anti-CD20 antibody immunomarking, 200",yes
PMC10731157,Figure_10,oa_package/61/5e/PMC10731157.tar.gz,[],Figure 10 Contrast enhanced computed tomography in the sagittal plane with Maximum Intensity Projection reconstruction demonstrates venous stenosis (arrowhead) at the anastomotic site. The pancreatic graft is enlarged with inhomogeneous enhancement which likely reflects graft dysfunction.,yes
PMC11587679,Figure_2,oa_package/ec/8e/PMC11587679.tar.gz,"[' 2A-D for males) alongside their respective controls.', ' 2A).', ' 2B-C).', '001) analyzed by two-way ANOVA\nMitochondrial proteinsIn an effort to investigate how oxidative phosphorylation (OXPHOS) machinery is affected by sex and age during normal aging and the progression of AD, we examined several mitochondrial-associated proteins in the hypothalamus.']","Fig. 2 Oxygen consumption rates (OCR) in hypothalamic mitochondria, comparing 3xTg males with control males. Representative measurements of OCR in isolated hypothalamic mitochondria from 3xTg and age-matched control males at three different ages: 2 months ( ), 6 months ( ), and 13 months of age ( ). Calculated parameters, which were recorded at baseline and following the addition of ADP, oligomycin, FCCP, and rotenone/antimycin . Table provides a detailed summary of the bioenergetic parameters, including basal, coupled, maximal respiration, spare respiratory capacity, and coupling efficiency for both 3xTg and aged-matched control males at the ages of 2, 6 and 13 months. No significant variations were found in the bioenergetic profiles across different ages or between the 3xTg and control strains. All data are expressed as OCR in pmoles/min, and values are expressed as meanSD of =5 replicates (* 0.05, ** 0.01, and *** 0.001) analyzed by two-way ANOVA",yes
PMC9925838,Figure_2,oa_package/32/f4/PMC9925838.tar.gz,"['Two years after initiating dupilumab, she progressed to erythroderma of the trunk and extremities, clinically concerning for possible cutaneous T-cell lymphoma (CTCL) (, A and B).', 'Several months after starting methotrexate, dupilumab was discontinued, and within 6 months, there was a gradual complete clearance of lesions (, C).', 'Suspected drug hypersensitivity reaction to dupilumab.']",Fig 2 Suspected drug hypersensitivity reaction to dupilumab. A 72-year-old female with atopic and allergic contact dermatitis presented with widespread erythematous-violaceous indurated plaques on the trunk ( ) and extremities ( ) 2years after initiating dupilumab. Biopsies at 4 different sites showed a lichenoid granulomatous pattern. She was initially treated with acitretin and then methotrexate. Eventually dupilumab was discontinued and she had complete clearance approximately 6months later without relapse ( ).,yes
PMC10721326,Figure_3,oa_package/65/64/PMC10721326.tar.gz,"['In contrast, no correlation was observed between CcO activity and synaptosomal A 40 (Supplemental , A C), brain A 42 (Supplemental , D F), or brain A 40 (Supplemental , G I) in any tested group.', 'As expected, ELISAs for A 40 and A 42 in brain homogenates showed the expression of human form A 40 (A) and A 42 (B) with nondetectable murine A in hA -KI mice.', 'In contrast to no age effect on brain A 40 (A), hA -KI mice surprisingly displayed an age-dependent decrease in brain A 42 (B).', 'Synaptic mitochondria in hA -KI mice had a stable amount of A 40 during aging (C).', 'However, in contrast to the age-dependent reduction in brain A 42 (B), synaptic mitochondrial fractions from hA -KI mice demonstrated increased A 42 in an age-dependent manner (D).', 'Notably, nonsynaptic mitochondria from hA -KI mice did not exhibit any age-related changes in A 40 (E) or A 42 (F), indicating that accumulation of A 42 predominantly develops in synaptic mitochondria.', 'In addition, as compared with their nonTg counterparts, synaptic mitochondria from hA -KI mice had increased A oligomerization, determined by dot-blotting with A11 (, G and H).', 'Although nonsynaptic mitochondria from younger hA -KI mice did not differ from their nonTg counterparts in A oligomerization (, I and J), elevated A oligomerization in nonsynaptic mitochondria from hA -KI mice became evident with mouse aging (, I and J).', 'Brain and mitochondrial A in hA -KI mice at ages 12 14 months and 20 22 months.']","Figure 3 Brain and mitochondrial A in hA-KI mice at ages 1214 months and 2022 months. ( ) A40 levels in brain homogenates from hA-KI mice. Two-tailed test with Welchs correction. Age 1214 months, = 5; 2022 months, = 6. ( ) A42 level in brain homogenates from hA-KI mice. Two-tailed Mann-Whitney test. Age 1214 months, = 5; 2022 months, = 6. ( and ) A40 ( ) and A42 ( ) levels in synaptic mitochondria from hA-KI mice. Unpaired 2-tailed test. Age 1214 months, = 9; 2022 months, = 7. ( and ) A40 ( ) and A42 ( ) levels in nonsynaptic mitochondria from hA-KI mice. Unpaired 2-tailed test ( ) and 2-tailed test with Welchs correction ( ). Age 1214 months, = 9; 2022 months, = 7. ( and ) A oligomers in synaptic mitochondria. ( ) Representative images of A11 and Tom40 dot blotting; ( ) A oligomers labeled by A11 Ab. Age 1214 months: unpaired 2-tailed test, nonTg, = 7; hA-KI, = 5. Age 2022 months: 2-tailed test with Welchs correction, = 6 each group. ( and ) A oligomers in nonsynaptic mitochondria. ( ) Representative images of A11 and Tom40 dot blotting; ( ) A oligomers labeled by A11 Ab. Unpaired 2-tailed test. Age 1214 months: nonTg, = 7; hA-KI, = 5. Age 2022 months: nonTg, =5; hA-KI, = 6. * < 0.05, ** < 0.01.",yes
PMC5198848,Figure_2,oa_package/45/71/PMC5198848.tar.gz,"['A new CT scan performed seven days later showed complete resolution, with total absence of gas within the portal derivations both extra- and intra-hepatic ().']","Fig. 2 (A and B) Abdominal CT scan (sagittal and coronal view), performed seven days later shows total absence of gas within the portal derivations both extra- and intra-hepatic.",yes
PMC5519492,Figure_4,oa_package/b1/49/PMC5519492.tar.gz,"['Here again, we found a significant decrease in vGlut1 and PSD95 (s 4A 4C).', 'Since demyelination can occur in many neurodegenerative disorders, we also assayed levels of myelin-binding protein (MBP) isoforms and found a significant decrease (s 4A and 4D).', 'The decrease in PSD95 was significant despite no changes in MAP2 levels, indicative of a selective synapse loss rather than general neuronal death (s 4A and 4E).', 'Consistent with these findings, we observed a significant decrease in cortical dendritic spine density in cKO mice (s 4F and 4G).', 'vGlut1 immunohistochemistry also revealed a significant decrease in mice depleted of microglial TDP-43 compared to controls (s 4H and 4I).', ' 4Selective Depletion of TDP-43 from Microglia Results in Enhanced Synaptic Loss in Mice Even in the Absence of Amyloid(A E) Representative blots of synaptic markers in the motor/somatosensory cortex of WT and cKO 8-month-old mice (A) and relative quantification for vGlut-1 (B), PSD95 (C), MBP (D), and MAP2 (E) normalized to -actin reference gene.', 'There was a significant increase in the fraction of synaptic marker engulfed by TDP-43 depleted microglia compared to WT controls (s 4J and 4K).', 'We also observed a significant increase in the phagocytic marker CD68 ( 4L).', 'The cells had increased size and total volume of CD68-positive structures, despite no change in number of structures (s 4M 4O).']","Figure4 Selective Depletion of TDP-43 from Microglia Results in Enhanced Synaptic Loss in Mice Even in the Absence of Amyloid (AE) Representative blots of synaptic markers in the motor/somatosensory cortex of WT and cKO 8-month-old mice (A) and relative quantification for vGlut-1 (B), PSD95 (C), MBP (D), and MAP2 (E) normalized to -actin reference gene. Mean SEM, n= 34 mice per genotype, p< 0.05, p< 0.01, unpaired two-tailed t test. (F and G) Representative confocal micrograph of dendritic spines from motor/somatosensory cortex of WT and cKO mice (F) (scale bar: 10m), and relative quantification (G) (WT n= 53, cKO n= 47 segments, from 4 animals per genotype). (H and I) Representative 3D reconstruction of vGlut1 immunoreactive puncta in the somatosensory cortex of WT and cKO mice (H) (scale bar: 15m), and relative quantification (I) (WT n= 8, cKO n= 10 acquisitions from 3 animals per genotype; p< 0.05, using two-tailed t test). (J and K) Representative 3D reconstruction of single microglia cells engulfing PSD95 (J) and quantified as fraction of engulfed PSD95 normalized to microglia volume (K) (means SEM, WT n= 12 and cKO n= 12 cells from 3 animals per genotype; p< 0.05, using two-tailed t test). (LO) Representative 3D reconstructions showing CD68-positive structures within Iba1-microglia cells (L) (scale bar: 10m). Quantification of CD68 structures total volume per cell (M), average size per CD68-structure (N), and number of CD68-positive structures per cell (O) (WT n= 16 and cKO n= 20, from 3 animals per genotype).",yes
PMC6906797,Figure_2,oa_package/15/55/PMC6906797.tar.gz,"['It was hypermetabolic with an SUV of 19 on PET CT ().', '001""/>PET CT showing a hypermetabolic right upper lobe nodule.']",Figure 2 PET CT showing a hypermetabolic right upper lobe nodule.,yes
PMC3863500,Figure_6,oa_package/45/40/PMC3863500.tar.gz,"['Arthroscopic-assisted and all-arthroscopic (s 6 and 7) technique [3, 62, 63] for snapping scapula syndrome rely on similar principles as the open procedures, and a comprehensive understanding of the anatomy described above is critical to avoid iatrogenic damage to the periscapular neurovascular structures or underlying chest wall.', '005""/>Arthroscopic images ((a), (b), and (c)) demonstrating arthroscopic bursectomy for snapping scapula syndrome with the use of an arthroscopic shaver (asterisks represent areas of inflamed bursa).']","Figure 6 Arthroscopic images ((a), (b), and (c)) demonstrating arthroscopic bursectomy for snapping scapula syndrome with the use of an arthroscopic shaver (asterisks represent areas of inflamed bursa).",yes
PMC4899427,Figure_2,oa_package/49/f3/PMC4899427.tar.gz,"['Final pathology returned as moderately differentiated clear cell carcinoma of the ovary limited to the left ovary with ovarian surface involvement (A-C).', 'The immunohistological features of the tumor including hyalinized papillary architecture lined by cytologically atypical cells with clear cytoplasm with lack of immunohistochemical evidence of p53 mutation or estrogen receptor (ER) expression, are typical of clear cell carcinoma (B-C).', 'Incidentally, endometriosis was noted on the ovaries bilaterally (D).', 'Photomicrographs of left ovarian clear cell carcinoma (A C) and right ovarian endometriosis (D).']","Fig. 2 Photomicrographs of left ovarian clear cell carcinoma (AC) and right ovarian endometriosis (D). Prominent hyalinized stroma is often seen in clear cell ovarian carcinomas. Neoplastic cells are noted to have distinct cell borders and a wide range of nuclear and cytologic atypia. , 400 H&E. , 400 p53 immunohistochemistry (negative). , 400 estrogen receptor immunohistochemistry (negative). , 400 H&E.",yes
PMC11260612,Figure_2,oa_package/83/be/PMC11260612.tar.gz,[],"Figure2 Chest CT of the patient. Plain CT on November 18, 2021 and contrast enhanced CT on November 28 . Patches of ground glass opacity can be observed around the lesion on image .",yes
PMC3303905,Figure_10,oa_package/ba/7f/PMC3303905.tar.gz,[],Fig. 10 Sterile drape (arrow) was placed to support needle in proper angulation during biopsy. Biopsy revealed squamous cell carcinoma.,yes
PMC10190694,Figure_6,oa_package/5f/47/PMC10190694.tar.gz,['\nRejection cholangiopathy.'],Figure 6 A: Inflammatory damage of interlobular bile duct (arrow) in acute T cell-mediated rejection; B: Senescence-related changes of the interlobular bile duct in chronic rejection. Haematoxylin and eosin; bar corresponds to 50 m (A) and 25 m (B).,yes
PMC6691096,Figure_2,oa_package/1f/d0/PMC6691096.tar.gz,"['The vagal nerve afferents tend to relay pro-inflammatory responses cells from organs in the periphery to the CNS () (68).', 'Increased pro-inflammatory molecules in the brain.']","Figure 2 Increased pro-inflammatory molecules in the brain. Schematic of vagal afferent and efferent modulation of inflammation. The vagal afferents mediate pro-inflammatory signals, such as IL-1 and TNF- from the periphery including the peritoneum and organs such as the lung intestine, hear, spleen, liver, and lung to stimulate inflammatory cytokines in the nucleus tractus solitarius (NTS). The NTS has projections to multiple brain areas where this pro-inflammatory signal that originates in the periphery leads to enhanced pro-inflammatory cytokine expression in brain areas that affect fatigue and sleep. Conversely, stimulation of the vagal efferents, such as that occurring from cholinergic mechanisms in the brain, such as muscarinic acetylcholine receptors (mAChR) acting through the, NTS, dorsal motor nucleus (DMN), and nucleus ambiguus (NA) can lead to anti-inflammatory reactions in peripheral tissues. The vagal afferents could serve to transfer enhanced inflammatory signals in the periphery occurring from autoimmune and related pathologies into dysregulated inflammatory regulation in the brain to induce fatigue-like behavior. Additionally, abnormal vagal efferent activity could server to affect physiological functions mediating fatigue-like behavior, such as heart rate, bronchoconstriction, and gluconeogenesis.",yes
PMC4349956,Figure_3,oa_package/34/5e/PMC4349956.tar.gz,"[' 3).', '3Numbers of scientific abstracts accepted by EANM 2014 per country in Europe, normalized per 10 million inhabitants\nRelative change (%) in number of scientific abstracts accepted by EANM for European countries over the past decade\n']","Fig. 3 Numbers of scientific abstracts accepted by EANM 2014 per country in Europe, normalized per 10 million inhabitants",yes
PMC9590410,Figure_5,oa_package/9f/13/PMC9590410.tar.gz,"['0001), see .', '6 years\n\nCranium morphology\nDolicho-/mesocephalic1562017610121122Brachycephalic1411520020Transverse plane MR images of various dog (A D) and cat (E H) brains at the level of the rostral commissure with bilateral dilated VRS (yellow arrowheads).', 'We furthermore noticed a less evident FLAIR hypointense signal intensity of the described dilated VRS in 1 Tesla acquired FLAIR images compared to 3 Tesla FLAIR sequences, see .']","Figure 5 Transverse plane MR images of various dog and cat brains at the level of the rostral commissure with bilateral dilated VRS (yellow arrowheads). T2-W and corresponding FLAIR sequences are shown. The top line demonstrates sequences acquired with a 3 Tesla scanner and the bottom line presents 1 Tesla studies. Note the hypointense signal intensity in FLAIR, confirming CSF-isointensity. Due to the decreased image resolution of the 1 Tesla FLAIR studies, the CSF-isointense foci cannot be detected .",yes
PMC8659598,Figure_12,oa_package/88/28/PMC8659598.tar.gz,[],"Figure 12 Ablation monitoring in a beating heart. ( ) 2 PA images before, during and after ablation, available as Movie 2. ( ) I790 equivalents. 2 PA data confirm lesion formation. ( ) Photograph of lesion made. ( ) Video endoscopy frame confirming a lesion was made. ( ) Sketch of instruments positions. Round inset: ICE-C and RFPA-C relative to the valve, oriented as in the images in ( , ). ICE catheter (ICE-C); PA-enabled ablation catheter (RFPA-C). Mitral valve (MV). Cyan arrows indicate indentation formed by ablation. Reprinted from [ ] with permission.",yes
PMC1805068,Figure_3,oa_package/2b/ca/PMC1805068.tar.gz,"['Second, by more accurately defining disease extent, it may obviate the need for expensive or morbid confirmatory tests that may otherwise be required when using a less accurate staging technique ().', 'Equivocal enlargement of mediastinal lymph nodes on diagnostic CT usually requires mediastinal lymph node sampling by various approaches including mediastinoscopy.']","Figure 3 Equivocal enlargement of mediastinal lymph nodes on diagnostic CT usually requires mediastinal lymph node sampling by various approaches including mediastinoscopy. These add to the cost and potential morbidity of the staging process. By identifying nodes that are more likely to be involved based on increased FDG uptake, and particularly more easily accessed, PET may improve the selection of the best nodal sites to sample in order to confirm, or exclude, metastatic involvement. In this case extrathoracic non-small cell lung cancer was confirmed by ultrasound-guided biopsy on a neck node identified on PET/CT and thus the patient was spared a surgical procedure.",yes
PMC8138723,Figure_2,oa_package/47/13/PMC8138723.tar.gz,"['Histopathologic examination revealed dense suppurative and granulomatous dermal inflammation containing hyphal forms ().', '']","Fig. 2 Histopathology demonstrating suppurative and granulomatous inflammation within the dermis with several fungal forms (hematoxylin-eosin, original magnification x100).",yes
PMC3955295,Figure_1,oa_package/c7/9d/PMC3955295.tar.gz,"['Sonography showed the heterogeneous mass with size of 124 61 mm expanding from the umbilicus to the bladder ().', '.']","Figure 1. Heterogeneous Mass in the Sonographic Examination M: Mass, B: Bladder",yes
PMC4283694,Figure_2,oa_package/e2/5b/PMC4283694.tar.gz,"['AIA and NBQX influence GluR expressionKA1 and AMPAR2 proteins were expressed in chondrocytes and synovial lining cells (not shown) in all rats, and localised to remodelling bone in AIA and AIA+NBQX (figure 2).', 'Osteocytes and other mononuclear cells in remodelling bone expressed AMPAR2 in AIA and AIA+NBQX (figure 2K,L).', 'NBQX reduced the extent of remodelling, with an apparent reduction of GluR positive cells (figure 2).', 'Neither AMPAR2 nor KA1 localised to mononuclear bone cells in naive animals (figure 2).', 'TRAP positive osteoclasts in AIA coexpressed KA1 and AMPAR2 in consecutive sections (figure 2).']","Figure2 KA1 and -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor 2 (AMPAR2) immunohistochemistry and tartrate resistant acid phosphatase (TRAP) staining in the lateral femoral condyle of naive, antigen-induced arthritis (AIA) and AIA+NBQX rats. Chondrocytes in all animals expressed KA1 and AMPAR2 (AC, GI, respectively, black arrows). Neither proteins localised to osteocytes or mononuclear bone cells (D, J, red arrow heads) in naive rats; however, in AIA and AIA+NBQX rats, AMPAR2 was expressed in osteocytes, mainly in areas of bone remodelling (K, L, red arrow). In AIA rats, mononuclear bone cells and areas of bone remodelling stained intensely for KA1 and AMPAR2 (B, E, H, K). AIA+NBQX rats showed less bone remodelling and subsequently less staining of both proteins (C, F, I, L, black arrow heads). Abundant TRAP staining was found in AIA rats (N) indicating the presence of more osteoclasts compared with naive (M) and AIA+NBQX rats (P). Consecutive sections showed expression of KA1 (E) and AMPAR2 (K) in TRAP positive osteoclasts (O) in AIA rats (blue arrows). Black boxes are shown at 40 in images underneath. (O) 40 Image of boxed area in N. Corresponding negative controls (no primary antibody) and rabbit IgG controls were negative for KA1 and AMPAR2 (see online S1). Scale bars: (AC, GI, M, N, P), 100m; (DF, JL, O), 50m.",yes
PMC3665260,Figure_2,oa_package/36/e4/PMC3665260.tar.gz,"['Thus, cystoid spaces on SDOCT may not necessarily correspond to fluorescein leakage on FA, but may represent structural defects indicating chronic disease ().', '001""/>Discrepancy between imaging modalities regarding identification of signs considered to indicate CNV activity: color fundus photography (a) demonstrates fibrosis and RPE changes, fluorescein angiography (b) demonstrates staining of CNV, and SDOCT (c) shows intraretinal cystoid spaces.']","Figure 2 Discrepancy between imaging modalities regarding identification of signs considered to indicate CNV activity: color fundus photography (a) demonstrates fibrosis and RPE changes, fluorescein angiography (b) demonstrates staining of CNV, and SDOCT (c) shows intraretinal cystoid spaces.",yes
PMC11006902,Figure_4,oa_package/b3/0e/PMC11006902.tar.gz,"[' 4 provides a visualization of the different types of processing and how they are combined to create input images for training and evaluation.', 'The three types of image processing used in the varied-input-processing ensemble models.', 'The two additional variations go an additional step by utilizing the pixel-level segmentation information from the U-Net from the previous pre-processing stage.', ' 4, requiring at minimum three trained models trained on each of the input processing variations.', ' 4).', ' 4 and varied input processing [*] uses one from each input processing type.', ' 4.', ' 4.']","Figure 4 The three types of image processing used in the varied-input-processing ensemble models. ( ) normal-cropped histogram equalized 3x-stacked images; ( ) segmentation-masked adaptive thresholding 3x-stacked images (binary); ( ) segmentation-masked raw, histogram equalized, and bilateral low-pass filtered (blended).",yes
PMC9482741,Figure_2,oa_package/5a/c8/PMC9482741.tar.gz,"[' 2).', 'Table 4Demographic, clinical, imaging, and management characteristics of the benign diseasesS noAgeGenderDiagnosisSurgeryMorphology of lesion in CECT Abdomen Constitutional symptoms Anorexia/ Weight lossGallstoneBile duct resection152MXanthogranulomatous cholecystitisRCMass formingNoYesN249FXanthogranulomatous cholecystitisRCThickeningNoNoY360FXanthogranulomatous cholecystitisRCMass formingYesYesY448FXanthogranulomatous cholecystitisRC + segmental colectomyThickeningNoNoN559FXanthogranulomatous cholecystitisRCMass formingYesYesN670MXanthogranulomatous cholecystitisRCThickeningNoNoN760MXanthogranulomatous cholecystitisRC + D1 duodendectomyThickeningNoYesN830MXanthogranulomatous cholecystitisRCThickeningNoNoN960FXanthogranulomatous cholecystitisRCThickeningYesYesN1070MChronic cholecystitisRCThickeningNoYesN1152FChronic cholecystitisRCThickeningNoYesY1234FIgG4 related cholecystitisRCThickeningyesYesN1348MIgG4 related cholecystitisRCMass formingNoYesY1468MTuberculosisRC + D1 duodenectomy + segmemtal colectomyMass formingYesYesN1542FTuberculosisRCThickeningYesYesYRC Radical cholecystectomyPicture collage showing benign mimickers of the carcinoma gall bladder.', 'j Photomicrograph (40 ) reveals granulomas with caseous necrosis (yellow arrowhead) and multinucleated giant cells (black arrow)On the other hand, there were 38 (25.']","Fig. 2 Picture collage showing benign mimickers of the carcinoma gall bladder. . Contrast-enhanced computed tomogram (CECT) of the abdomen shows irregular heterogeneously enhancing thickened gall bladder wall forming a mass with loss of interface with liver (Yellow arrow). Resected specimen showing irregular thickened gall bladder infiltrating the liver bed (yellow arrow). Photomicrograph (400) reveals dense infiltration with plasma cells and lymphocytes. These plasma cells have eccentrically placed cartwheel-like nuclei with perinuclear hof. Immunohistochemistry image shows characteristics cell with strong cytoplasmic positivity for IgG4. : CECT of the abdomen shows irregular hypodense thickened gall bladder wall forming a mass with loss of interface with liver (Yellow arrow). Resected specimen showing irregular thickened gall bladder wall (yellow arrow). Photomicrograph (40) reveals Aschoff-Rokitansky sinus (thick yellow arrow), thickened gallbladder wall with myofibroblastic proliferation (black arrow) with infiltrates of lympho-histiocytes and chronic inflammatory cells (thin yellow arrow). CECT of the abdomen shows irregular heterogeneously enhancing thickened gall bladder wall with loss of interface with liver (Yellow arrow). Specimen showing multi-visceral resection including gall bladder mass (yellow arrow) with a colon (black arrow) and omentum. Photomicrograph (40) reveals granulomas with caseous necrosis (yellow arrowhead) and multinucleated giant cells (black arrow)",yes
PMC3551781,Figure_16,oa_package/2e/b4/PMC3551781.tar.gz,[],"Figure 16 ) Four cell embryo. and ) morula. and ) Blastocyst consisting of blastocoele (bc), trophectoderm (te) and inner cell mass (icm) releasing (arched arrow) DAZL+ embryonic stem cells (esc). Scale in C for A-C. A, C and F - Dazl staining, B, E and G - DAPI. Adapted with permission from[ ], Cambridge University Press.",yes
PMC6762198,Figure_4,oa_package/bf/3a/PMC6762198.tar.gz,"['s006"">S2 Data), Nlrp3A350V/+CreT+ macrophages that expressed Nlrp3A350V secreted high levels of IL-1 (A and S3 Data) and IL-18 (B and S3 Data) and IL-18 (B and S3 Data) secretion and Nlrp3A350V-driven cleavage of Casp1 and IL-1 (C and LPS + ATP and LPS + nigericin potently triggered IL-1 and IL-18 secretion and maturation of Casp1 and pro-IL-1 from both Nlrp3A350V/+CreT+ and Nlrp3A350V/+CreT control BMDMs (D 4I and Our results in Nlrp3A350V macrophages showed that this mutation subtly altered the ability of MCC950/CRID3 to inhibit inflammasome activation with potent inhibition seen only in response to LPS but not following signal 2 triggers such as ATP and nigericin ().', 's007"">S3 DataNumerical data underlying A, 4B, 4D, 4E, 4G and 4H.']",10.1371/journal.pbio.3000354.g004,yes
PMC8613496,Figure_1,oa_package/7b/14/PMC8613496.tar.gz,"['The abdomino-pelvic computed tomography scan and the magnetic resonance imaging (MRI) [] described a polylobulated pelvic mass originated in the right adnexal area, measuring 7.', 'T2-weighted magnetic resonance imaging revealing an ovoid solid tumor of the ovary (arrow) on sagittal section (a), axial section (b), and coronal section (c)A diagnostic laparoscopy was proposed and accepted by the patient.']","Figure 1 T2-weighted magnetic resonance imaging revealing an ovoid solid tumor of the ovary (arrow) on sagittal section (a), axial section (b), and coronal section (c)",yes
PMC11685000,Figure_1,oa_package/b7/27/PMC11685000.tar.gz,"[""IFN driven cutaneous manifestations of dermatomyositis (A D) and cutaneous lupus erythematosus (E H) are pictured: (A) widespread erythematous to violaceous papules and plaques on extensor surfaces; (B) heliotrope sign; (C) poikilodermatous erythema on anterior neck in V distribution; (D) Gottron's papules overlying joints; (E) psoriasiform SCLE; (F) malar erythema of acute LE; (G) annular erythematous plaques of SCLE; (H) dorsal hand erythema sparing joints of LE.""]","Figure 1 IFNdriven cutaneous manifestations of dermatomyositis (AD) and cutaneous lupus erythematosus (EH) are pictured: (A) widespread erythematous to violaceous papules and plaques on extensor surfaces; (B) heliotrope sign; (C) poikilodermatous erythema on anterior neck in V distribution; (D) Gottron's papules overlying joints; (E) psoriasiform SCLE; (F) malar erythema of acute LE; (G) annular erythematous plaques of SCLE; (H) dorsal hand erythema sparing joints of LE. IFN, interferon; LE, lupus erythematosus; SCLE, subacute cutaneous lupus erythematosus.",yes
PMC11557572,Figure_6,oa_package/b8/e5/PMC11557572.tar.gz,"['6a-f, miR-26a-5p, miR-320a-3p, and miR-106a-5p are significantly lower expressed in the mesenchymal cells, while miR-21-5p and miR-34a-5p are increased.', '6g).', '\nmiRNAs associated with the EMT process.', '\nThese results led us to modulate miR-26a-5p in these cellular models to test whether this miR could reverse the CDH1-CDH2 switch.']","Fig. 6 miRNAs associated with the EMT process. Cluster expression levels of ( ) miR-26a-5p, ( ) miR-21, ( ) miR-34a-5p, ( ) miR-320a-5p, ( ) miR-106a-3p and ( ) miR-424-5p as analyzed by using qRT-PCR. Data are shown as meansSD ( =4) ( ) Pearsons correlation matrix of EMT markers and the miRNA cluster. <0.05 is considered statistically significant.",yes
PMC11379749,Figure_3,oa_package/8d/b5/PMC11379749.tar.gz,"['3 and 4).', '', ')']",Fig.3 Adult patient s/p Rastelli procedure with prosthetic pulmonary valve. Cardiac CT video clip demonstrating prosthetic pulmonary valve with mobile vegetation. Chest CT with multiple septic pulmonary emboli. (Courtesy of Dr. Dan Wallihan.),yes
PMC9577133,Figure_2,oa_package/92/01/PMC9577133.tar.gz,"['Representative examples of annotation for various images were shown in A C.', 'Table 1Number of picturesNumber of annotationsBenignNon-invasive carcinomaInvasive carcinomaTotalBenignNon-invasive carcinomaInvasive carcinomaTotalTraining60833741613613516148512946295Test164401113158531542471254Examination set-114151443 Examination set-214151443 Example images of annotation by pathologists and detection by the trained SSD model.', 'Then, a single-label diagnosis of the whole image (image label) was provided for each image.', 'An example image of accurate detection by the trained model was shown in A.', ' Detection with higher confidence score was used for diagnosis and that with lower confidence score was not used, if some boxes with different labels were detected in the same region (B).', 'For example, the model accurately detected the benign area by bounding boxes with different shapes against the annotation boxes as shown in C.']","Fig. 2 Example images of annotation by pathologists and detection by the trained SSD model. Examples of the annotation (left) and the model detection (right). The blue, red, and green boxses indicated benign, non-invasive carcinoma, and invasive carcinoma, respectively. : The image of accurately detection by the model. : The image that some boxes with different labels were detected in same region. : The image that the model accurately detected the benign area by bounding boxes with different shape against the annotation.",yes
PMC10509342,Figure_5,oa_package/dd/42/PMC10509342.tar.gz,[],FIGURE 5 PET scan showing 18fluorodeoxyglucose uptake along the middle skull base and extending toward the left side (simple arrow). Note that posteriorly the cerebellum is highlighted by a normal 18fluorodeoxyglucose uptake (double arrows).,yes
PMC6751859,Figure_2,oa_package/52/b6/PMC6751859.tar.gz,"[' 2a).', ' 2b) [52].', ' 2c).', '', 'The phenotype and ADCC function is then assessed via surface markers, CD107a as a marker for degranulation and IFN by intracellular cytokine staining\nRecently, it was demonstrated that adaptive NK cells correlated with protective malaria phenotypes, including decreased parasitaemia and reduced symptoms [35].', ' 2d) [35].']","Fig.2 Assays to look at Natural Killer cell function. Model of the subset of adaptive NK cells that lack Fc receptor chain. These cells are particularly skilled at degranulating and producing IFN that can help activate the immune response to Growth inhibition antibody dependent cellular cytotoxicity assay (Alternative GI-ADCC assay). Synchronized late stage parasite culture is incubated with or without natural killer cells and with or without immune plasma or antibodies. In this assay, the NK cells can kill the infected RBCs but the antibody is still there to allow for growth inhibition via neutralizing activity against merozoites. The resulting inhibition can then be quantified in all groups and compared by looking at parasitaemia. Growth inhibition antibody dependent cellular cytotoxicity assay (GI-ADCC assay). In this assay late stage purified (>95% pure) infected RBCsare mixed with NK cells with or without immune plasma or antibodies. After 5h the antibodies are washed out, then uninfected RBCs are added at 100 fold excess. The infected RBCs then rupture and the resulting parasitaemia the next day is used to assess growth inhibition. Functional analysis of NK cells in mixed PBMCs. PBMCs are incubated at a 1:1 ratio with late stage purified infected RBCs then immune or naive plasma is added. The phenotype and ADCC function is then assessed via surface markers, CD107a as a marker for degranulation and IFN by intracellular cytokine staining",yes
PMC6051668,Figure_4,oa_package/d7/d7/PMC6051668.tar.gz,"['5 control and Nkx2-5 TrbE10 hearts by PH3 immunofluorescence on sections (A and 4B).', 'Using Cx40-Cre::R26-YFP genetic tracing of the same fetal hearts, we observed no difference in the distribution of YFP+ cells in mutants in either atria and ventricles (B).', '5 control and Nkx2-5 TrbE10 hearts (C).', 'g004Analysis of proliferation and the compact trabecular boundary in fetal mutant hearts.']",10.1371/journal.pgen.1007502.g004,yes
PMC6421483,Figure_4,oa_package/1a/93/PMC6421483.tar.gz,[],"Figure 4 Clear keratinocytes, Hematoxylin-eosin staining, 20x",yes
PMC5630294,Figure_7,oa_package/d2/49/PMC5630294.tar.gz,['The expressions of apoptosis-related proteins in the hippocampus tissues of rats in each group were detected by qRT-PCR and Western blottingNotes: A.'],"Figure 7 The expressions of apoptosis-related proteins in the hippocampus tissues of rats in each group were detected by qRT-PCR and Western blotting Notes: , The expressions of apoptosis-related proteins in the hippocampus tissues of each group of rats were detected by Western blotting; , The quantification of the western-blotting; , The expressions of the mRNA of apoptosis-related proteins in the hippocampus tissues of each group of rats were detected by qRT-PCR; *, < 0.05 as compared with the normal group; #, < 0.05 as compared with the model group; &, < 0.05 as compared with the empty vector group; $, < 0.05 as compared to the NC group; NC = negative control; qRT-PCR = quantitative real-time plymerace chain reaction.",yes
PMC10683101,Figure_1,oa_package/35/da/PMC10683101.tar.gz,"[' 1a).', ' 1b).', 'Clinical images.', 'd A nodular tumor approximately 3 cm in size appeared in the right pleura nine months from the first biopsyThe pleural effusion decreased after 5 months from the first biopsy (', ' 1c).', ' 1d).']","Fig. 1 Clinical images. Chest computed tomography showed a moderate amount of right pleural effusion with a slight bilateral pleural thickening. A video-assisted thoracoscopic surgery biopsy of the right parietal pleura showed plaque-like pleural thickening, but no visible tumor. The right pleural effusion decreased within five months from the first biopsy. A nodular tumor approximately 3cm in size appeared in the right pleura nine months from the first biopsy",yes
PMC9502604,Figure_2,oa_package/cd/7b/PMC9502604.tar.gz,"[' 2.', 'Image preprocessing on CT images for the deep learning analysis.', 'Finally, these processed images were fed into the data augmentation process with image rotation and/or a shift for training dataFor the preparation of the invasion-positive group in the training dataset, we included only the specific slices that were judged as invasion-positive in the slice-based evaluation (see above: Determination of the final diagnosis); this dataset consisted of 408 images from 56 lesions.']","Fig. 2 Image preprocessing on CT images for the deep learning analysis. First, a square region of interest (ROI) was manually placed to fully include the orbital wall on coronal CT images (red arrow). Next, segmented images by the ROI were extracted as continuous slices including the tumor. Then, right-side lesion images were inverted to the left side (flipped horizontally) and all images were aligned, as the lesion is shown at the left side. Thereafter, the top and outer areas in the image were masked (white asterisk). Finally, these processed images were fed into the data augmentation process with image rotation and/or a shift for training data",yes
PMC7399600,Figure_2,oa_package/cf/a6/PMC7399600.tar.gz,"['2, 3, 4) were the most frequent abnormal findings.', ' 2), pneumothorax (2%) (Figs.', 'Within the subpleural consolidation there were air bronchograms (arrow) and normal flow on colour DopplerChest CTTable 3 summarizes chest CT findings in 24 children with confirmed COVID-19.', '2), as a follow-up diagnostic procedure after CT (']","Fig. 2 A 4-year-old girl presented after 2days of fever and cough. Anteroposterior chest radiograph shows bilateral perihilar peribronchial thickening along with left upper and lower lobe focal airspace consolidations and moderate left pleural effusion ( ). , Coronal lung ultrasound image ( ) and coronal colour Doppler image ( ) show extensive subpleural consolidation within the posterobasal area of the left lung, as well as a simple pleural effusion ( ). Within the subpleural consolidation there were air bronchograms ( ) and normal flow on colour Doppler",yes
PMC8306898,Figure_2,oa_package/a5/fc/PMC8306898.tar.gz,"['A shows a significant increase of cell death in the presence of A 25 35 as compared to control cells, whereas URB597 reversed A 25 35-induced cell death.', 'Treatment with URB597 alone did not interfere with cell survival and did not prevent A 25 35 challenge at the mitochondrial level (B).', 'Effects of A 25 35 in the presence or absence of URB597 on BV-2 cells.']","Figure 2 Effects of A in the presence or absence of URB597 on BV-2 cells. ( ) Trypan blue exclusion test. Cell count was determined in BV-2 cells exposed for 24 h to 30 M of A in the presence or absence of 5 M of URB597 and expressed as cell death (cell death/cell death + living cell). Data are reported as percentage versus CTRL. ( ) Analysis of cell viability was evaluated by MTT assay. MTT reduction was analyzed in the same samples of treated cells at 24 h and 48 h. Data are expressed as percentage versus CTRL. The values are the mean SEM of triplicate determination from independent experiments. < 0.05, < 0.01, < 0.0001.",yes
PMC4867056,Figure_2,oa_package/65/6d/PMC4867056.tar.gz,"['As shown in , there was significant difference between normal and abnormal groups.', '001""/>Bone microstructure changes of tibia after OVX or ORX.']","Figure 2 Bone microstructure changes of tibia after OVX or ORX. Histomorphological photomicrographs: (a) control mice, (b) model mice; The CT-scanning images: (c) control mice, (d) model mice.",yes
PMC9705147,Figure_7,oa_package/e4/3f/PMC9705147.tar.gz,"['9\n\nOn imaging, ischiofemoral impingement manifests as a reduction in the ischiofemoral space measured between the ischium and lesser trochanter, with edema within the quadratus femoris muscle\n10\n(\n\n).', '\nIschiofemoral impingement.']",Fig. 7 Ischiofemoral impingement. Axial proton density fat-suppressed image showing marked narrowing of the ischiofemoral space ( ) with mild edema in the quadratus femoris muscle ( ).,yes
PMC5059137,Figure_10,oa_package/9a/15/PMC5059137.tar.gz,[],10.7554/eLife.15461.012,yes
PMC4433140,Figure_6,oa_package/08/3c/PMC4433140.tar.gz,"['g005""/>Histologic and molecular phenotyping show similarities in FGF9 overexpressing mouse lung and human Type I PPBThe pathologic findings in Type I PPB showed a characteristic expansion of primitive or uncommitted mesenchyme (A) associated with a benign-appearing Nkx2.', '1 positive epithelium (B).', 'g006Type I PPB and induced late gestation expression of epithelial FGF9 in mice have similar histopathology and cell differentiation.', 'However, vascular SMA expression appeared normal in both mouse and human tissue (I, black arrowhead).']",10.1371/journal.pgen.1005242.g006,yes
PMC7647030,Figure_2,oa_package/fa/eb/PMC7647030.tar.gz,"['Hippocampal tissues in the Sham group demonstrated normal histology with palely stained neuronal cells and round nuclei (A).', 'Compared with the Sham group, various histopathological changes were observed in the CIRI group, such as nuclear pyknosis, a decrease in neuronal numbers and neuron atrophy (B).', 'When mice were treated with a L-dose of Pic, the number of hippocampal neurons increased and neuronal atrophy was alleviated, although it did not reach the level of the Sham group (C).', 'Moreover, hippocampal tissues in the Pic-H treated group had less pathological impairment (D).', '.']","Figure 2. Histological analysis of the effects of Pic on neuronal injury induced by CIRI in mice. Hematoxylin and eosin staining was performed on tissue sections from the hippocampal Cornu Ammonis 1 regions (magnification, 400; n=5 per group). (A) Neurons with normal histology were observed in the Sham group. (B) Altered neurons characterized by nuclear pyknosis, neurons decrease and neuron atrophy were found in the CIRI group. (C) Neuronal alterations were slightly eliminated in the Pic-L treatment group. (D) Neuronal alterations were significantly eliminated in the Pic-H treatment group. CIRI, Cerebral ischemia-reperfusion injury; Pic, Piceatannol; L, low; H, high.",yes
PMC8160561,Figure_22,oa_package/9c/f7/PMC8160561.tar.gz,[],Fig.22 Histoplasmosis: 62-year-old female patient receiving methotrexate and TNF-alpha antagonist for treatment-resistant rheumatoid arthritis presented with fever and left upper quadrant pain. Axial plane T2W-fat-suppressed MR image showed several subcentimeter hypointense lesions scattered throughout the spleen (not shown). These lesions did not demonstrate any obvious enhancement in axial plane postcontrast T1W image (arrowheads). Percutaneous image-guided biopsy revealed non-necrotizing granulomas associated with budding yeast consistent with infection,yes
PMC6190812,Figure_2,oa_package/0c/80/PMC6190812.tar.gz,"['Biopsy showed mature adipose tissue with synovial lining, revealing the mass to be an intraarticular synovial lipoma ().']","Figure 2 Hematoxylin and eosinstain section of the left-knee mass The mass (pale tan tissue measuring 0.4 0.4 0.1 cm) was biopsied anteriorly from between the anterior cruciate ligament and the posterior cruciate ligament. This was one of three masses removed during knee arthroscopy, synovial biopsy, and synovectomy. The section shows mature adipose cells with overlying synovial lining. The infiltration and presence of inflammatory cells in this specimen is consistent with an inflamed synovial lipoma.",yes
PMC4664485,Figure_7,oa_package/8d/eb/PMC4664485.tar.gz,"['In the areas of florid hyperplasia, the epithelium piled up into multiple disorganized layers and formed small nests and acini without breaching the basement membrane (dysplasia; ).', 'g007Photo of hematoxylin and eosin stained histopathology slide of ceruminous gland dysplasia in a Santa Catalina Island fox (main image scale bar = 200 m, inset = 50 m).']",10.1371/journal.pone.0143211.g007,yes
PMC9632977,Figure_2,oa_package/36/c8/PMC9632977.tar.gz,['Magnetic resonance imaging (MRI) demonstrated jejunal intussusception of the left-sided upper abdomen with concentric circle-like changes.'],"Figure 2 Magnetic resonance imaging (MRI) demonstrated jejunal intussusception of the left-sided upper abdomen with concentric circle-like changes. ( ) T1 FS (arrow); ( ) RTr OAx T2 fs (arrow); ( ) WATER: Ax LAVA-Flex (arrow); ( ) InPhase: Ax LAVA-Flex (arrow); ( ) BH Cor 2D Fiesta Shim showed the intussusception of the long segment of jejunum from the pelvis to the left-sided upper abdomen, with concentric circle-like changes about 14.2cm (arrows); ( ) BH Cor 2D Fiesta Shim showed the thickening of the proximal jejunum intestinal wall with the thickest part about 2.6cm, and the lumen was narrowed with peripheral exudation (arrow). The distal segment of the jejunum and ileum were dilated, with fluid accumulation and slightly thickened walls (circle).",yes
PMC9380890,Figure_3,oa_package/f7/2b/PMC9380890.tar.gz,"['We performed double immunofluorescent staining for ChAT to visualize Vmo neurons and for VGluT1 (A).', 'As shown in B, the density of VGluT1-immunopositive axon varicosities in the dorsolateral Vmo was significantly reduced in 3 Tg-AD mice compared to NonTg mice, suggesting that information input from Vmes neurons to Vmo neurons is reduced.', 'The density of VGluT1-immunopositive varicosities in the Vmo.', 'Indeed, the density of VGluT1-positive axon terminals derived from the Vmes was reduced in the Vmo of 3-month-old 3 Tg-AD mice ().']","Figure 3 The density of VGluT1-immunopositive varicosities in the Vmo. Representative confocal image of the center of dorsolateral Vmo. Motor neurons in the Vmo were labeled with ChAT immunoreactivity (green). In the Vmo, VGluT1-immunopositive varicosities (magenta) derived from the Vmes are located only in the dorsolateral part of the Vmo innervating the jaw-closing muscles. The density of VGluT1-immunopositive varicosities appeared to be decreased in 3 Tg-AD mice than in NonTg mice. The 3-month-old 3 Tg-AD mice ( = 5) showed a significant reduction in the density of VGluT1-immunopositive varicosities compared to NonTg mice ( = 4) (* = 0.0402, two-tailed unpaired -test). Scale bar: 10 m .",yes
PMC7588970,Figure_6,oa_package/62/90/PMC7588970.tar.gz,"['sGFP was not detected in the ToTVpJL-Kra- or mock-infected plants ().', 'Detection of sGFP accumulation in Nicotiana benthamiana infected with ToTVpJL-KraGFP or ToTVpJL-Kra.']","Figure 6 Detection of sGFP accumulation in infected with ToTV -Kra or ToTV -Kra. Plant material infected with ToTV -Kra (1), ToTV -Kra (2), or mock-treated plants (3) were collected and subjected to anti-GFP immunoprecipitation (IP) using GFP-Trap Magnetic Agarose. Protein samples eluted from agarose as well as protein input collected before and after GFP-IP were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by protein staining ( ) and immunoblot ( ) analyses. kDakilodalton.",yes
PMC11471187,Figure_3,oa_package/f7/55/PMC11471187.tar.gz,"['However, lesions on the medial side of the pulvinar nucleus is to be surgically resected through supra-cerebellar-infratentorial approach9 ().', 'An artistic depiction of the supero-lateral view of the right AC showing the neurovascular structures and the related brainstem parts.', '7Imaging of AWCThe wing of the ambient cistern (AWC) can be pictured using multiple imaging modalities, surprisingly many of the old imaging techniques that are no longer used were able to clearly visualize it.']","Fig. 3 An artistic depiction of the supero-lateral view of the right AC showing the neurovascular structures and the related brainstem parts. (Basilar artery), (Oculomotor nerve), (Trochlear nerve), (Midbrain), (anterior pontomesencephalic artery), (Superior cerebellar artery rostral and caudal trunks), (Superior cerebellar artery), (Tentorial edge), (Trigeminal nerve).",yes
PMC10421797,Figure_26,oa_package/1f/fe/PMC10421797.tar.gz,[],"Fig. 26 Sagittal magnetic resonance images of the spine in a 5-year-and-3-month-old girl with tuberculous meningitis andprogressive lower limb weakness. T2 image shows a hypointense posterior epidural mass extending from T2 to T9 ( ) andcompressing the cord anteriorly. There is cord oedema proximal to the mass ( ). , T1 pre- ( )and post- ( ) gadolinium show the mass to be homogeneously enhancing ( ). There is also nerve root enhancement ( )",yes
PMC8362913,Figure_1,oa_package/cd/fc/PMC8362913.tar.gz,"['According to the Savary Miller classification of the oesophagus severity, a fibroesofagoscopy revealed catarrhal oesophagitis in 84 (66%) patients, traces of erosion in 12 (9%) and fibrinous erosive oesophagitis in 18 (14%) children [].', 'Erosive oesophagitis fibroesophagoscopy: (a) Expansion of the cardiac outlet zone (b) Traces of erosionPeptic stricture of the oesophagus.']",Figure 1 Erosive oesophagitis fibroesophagoscopy: (a) Expansion of the cardiac outlet zone (b) Traces of erosion,yes
PMC8321042,Figure_3,oa_package/b1/86/PMC8321042.tar.gz,"[' shows an example of the decomposition of two slides into tiles.', 'An example of slides from our dataset in which we applied a rectangular grid (shown with the continuous black lines) to show the division of a slide in tiles.']",Figure 3 An example of slides from our dataset in which we applied a rectangular grid (shown with the continuous black lines) to show the division of a slide in tiles.,yes
PMC6137340,Figure_4,oa_package/c4/02/PMC6137340.tar.gz,"['An unruptured left M1 middle cerebral artery aneurysm was additionally identified ().', 'Following endovascular coiling, the patient was transferred to theatre for extraventricular drain insertion by the on-call neurosurgical team.']",Fig. 4 Coronal planar cerebral DSA following coiling. The arrow identifies an incidental left M1 middle cerebral artery aneurysm.,yes
PMC3617144,Figure_1,oa_package/af/11/PMC3617144.tar.gz,"['ResultsReduction of the asthmatic phenotype in 5i KO mice in experimental OVA/Alum modelTo investigate the role of 5i subunit of the immunoproteasome, C57BL/6N (Wt) and 5i KO mice were sensitized and challenged either with PBS/OVA or OVA/OVA as shown in A.', 'In comparison to Wt mice, 5i KO mice demonstrated reduced recruitment of inflammatory cells as shown by the lower number of eosinophils, lymphocytes and neutrophils in the BAL (B).', 'Further, OVA specific IgG1 titers (C) and the Th2 cytokines IL-4 and IL-13 produced by BALs were significantly decreased in 5i KO mice as compared to Wt controls (D).', 'g001OVA/Alum acute asthma is reduced in 5i KO mice.']",10.1371/journal.pone.0060565.g001,yes
PMC7588064,Figure_4,oa_package/d3/37/PMC7588064.tar.gz,"['TiPV replication and distribution in naturally infected tilapiaThe localization of viral mRNAs of TiPV in infected tilapia was investigated by In situ hybridization (ISH) and shown in .', 'The strongest signals were observed in the kidney and spleen (A and 4B).', 'No signal was detected in negative control samples (Aa and 4Ba).', 'g004TiPV replication and viral protein expression in naturally infected tilapia.', 'As results show in , strong signals could be detected in kidney and spleen tissues, and the TiPV infected cells were antigen-positive and exhibited bright red fluorescence in these tissues (C and 4D).', 'In addition, no specific fluorescent signal was observed in uninfected tissues and the cell nuclei stained uniformly with DAPI (Ca and 4Da).']",10.1371/journal.ppat.1008765.g004,yes
PMC6744721,Figure_1,oa_package/a9/bc/PMC6744721.tar.gz,"['Computer tomography and magnetic resonance findingsThe brain computer tomography (CT) revealed a suprasellar cistern and anterior interhemispheric SAH with IVH; this required placement of a right ventricular drain [].', ':(a) Axial noncontrast head computer tomography showing significant bilateral frontal horn, third and fourth ventricular hemorrhage.']","Figure 1: (a) Axial noncontrast head computer tomography showing significant bilateral frontal horn, third and fourth ventricular hemorrhage. (b) Sagittal noncontrast head computer tomography showing third and fourth ventricular hemorrhage. The lateral ventricles are not well visualized here, but some intraventricular hemorrhage is still seen.",yes
PMC3579986,Figure_9,oa_package/02/80/PMC3579986.tar.gz,"[' 9).', 'Imaging also confirms the presence of hepatic metastases which were covert on a previous contrast enhanced CT scan\nTherapeutic options in the treatment of DSRCTs are limited.']","Fig. 9 Coronal F-FDG PET/CT imaging in a 21-year-old male patient with DSRCT. This image demonstrates the extent of disease with multiple areas of FDG uptake within the abdomen, which correspond to peritoneal deposits and retroperitoneal lymph node disease on the CT component. Imaging also confirms the presence of hepatic metastases which were covert on a previous contrast enhanced CT scan",yes
PMC8817813,Figure_5,oa_package/5a/7a/PMC8817813.tar.gz,"['org/1999/xlink"" xlink:href=""10-1055-s-0041-1735916-i2120025-4""/>\nBreast metastasis.']",Fig. 5 Breast metastasis. Anteroposterior radiograph ( ) and positron emission tomographycomputed tomography (PET-CT) ( ) showing left acromial metastasis with increased uptake on PET-CT (arrow).,yes
PMC3313852,Figure_1,oa_package/6c/56/PMC3313852.tar.gz,['L.'],"Figure 1 . Sera were collected at the time points indicated from -inoculated pigs and IgA and IgG was measured by ELISA (diluted 1:12.5 and 1:50, respectively). Values from individual pigs in group RE, TC, and CC are expressed as OD% of positive control sera of individual animals. Arrows indicate inoculation with at day 0 and at day 49.",yes
PMC10623738,Figure_1,oa_package/d4/f6/PMC10623738.tar.gz,"[' 1).', 'Transverse view of the right globe demonstrating a hyperechoic density, as seen at the arrow tip, consistent with a cataractOphthalmology was consulted, and the case was reviewed; they believed that given the patient s history, clinical presentation, physical examination, and ultrasound findings, symptoms were most likely secondary to a mature cataract of the right eye.']","Fig. 1 Transverse view of the right globe demonstrating a hyperechoic density, as seen at the arrow tip, consistent with a cataract",yes
PMC7653184,Figure_5,oa_package/0e/04/PMC7653184.tar.gz,['\nFluoroscopic views of the rectal region status post anastomosis.'],"Figure 5 A tiny focal area of extraluminal contrast (blue arrow), noted in the right posterior aspect of the rectum near the suture line.",yes
PMC7666849,Figure_13,oa_package/67/bb/PMC7666849.tar.gz,[],Figure 13 Percentage frequencies of different abnormalities evaluated in tissues from fatal cases,yes
PMC9484175,Figure_5,oa_package/72/da/PMC9484175.tar.gz,"['5a, b).', 'Microscopic aspects of special HCC variants.', 'i A FLC showed intra-tumoral pale bodies (IHC, 200 )Clear cell HCCThis variant is defined as 80% of tumor cells being clear cells.', '5c, d).', '5e, f).', '5g).', '5h, i).']","Fig. 5 Microscopic aspects of special HCC variants. A macrotrabecular massive subtype showed trabeculae > 10 cells in thickness (IHC, 100). A clear cell subtype showed sheets of hepatocytes contained high glycogen and lipid content (IHC, 100). A clear cell subtype showed focal fatty changes (IHC, 100). A clear cell subtype showed severe fatty changes (IHC, 100). A steatohepatitic subtype showed a triad of fatty change, intra-tumoral fibrosis, and inflammation (IHC, 100). A steatohepatitic subtype showed Mallory hyaline bodies (IHC, 200). A scirrhous subtype showed compressed cords of hepatocytes within desmoplastic stroma (IHC, 100). A FLC variant showed hepatocytes with abundant eosinophilic cytoplasm, prominent eosinophilic nucleoli separated by lamellated collagen bundles (IHC, 100). A FLC showed intra-tumoral pale bodies (IHC, 200)",yes
PMC7553959,Figure_1,oa_package/42/19/PMC7553959.tar.gz,"['1, 2a, b, d, 3 to 6, and iPSC line 2 was used in the caspase 3 activity assay shown in ', 'Characterization of human-induced pluripotent stem cells (iPSCs) and iPSC-derived cerebral organoids over the two-month differentiation process from iPSCs to cerebral organoids.', 'Characterization of ethanol-induced apoptosis and ultrastructure changes in cerebral organoids derived from human iPSC line 1 (B and D) and line 2 (C).', '1) as we and other described previously51,62 and in detail in Supplemental Materials and Methods.', '1A).', '1B), and an emergence of neuroepithelial stem cells and neurons.', '1C).', '1D-a).', '1D-b).', '1D-c) were also seen in 2-month-old cerebral organoids throughout the tissue.', '1E-a).', '1E-b and 1E-c, respectively).', '1F-a and b, respectively).', '1A).', '1B).', '1) as previously reported91,105,106.', 'pdf"">Supplemental 0.05; ***, P > 0.1. (E) Images show the distribution of internalized b-Cy3BTX (left) and mAb 210 antibodystained surface AChR (right) in cells transfected with Rac1 N17 (outlined) with respect to untransfected control cells. (F) BTX incubation of AChR-expressing CHO-K1/A5 cells with or without 10 M PP2 were lysed, and activated Rac1 was precipitated using PAK-PBD; total Rac1 and PAK-PBDprecipitated Rac1 were analyzed by Western blots from the same cell lysates. Note that BTX binding induces PP2-inhibitable Rac1 activation. Bars: (C) 20 m; (E) 10 m.",yes
PMC10436077,Figure_3,oa_package/44/f4/PMC10436077.tar.gz,[],"Figure3 Box and whisker plots of MRI markers of proton density fat fraction (PDFF, expressed as percentages) and transverse relaxometry (R2*, expressed as s ) versus histologic grades. Dark-yellow boxes correspond to liver PDFF and light-yellow boxes to pancreas PDFF. Dark-blue boxes correspond to liver R2* and light-blue boxes to pancreas R2*.",yes
PMC6747090,Figure_3,oa_package/07/3e/PMC6747090.tar.gz,"['Class IIb (HDAC 6 and 10) are primarily found in cytosol (A).', 'After 72 h, mRNA expression of each of HDACs was evaluated (B).', '(C,D).', 'Immunoblot analysis was followed to investigate tau acetylation levels upon HDAC6 knock-down (E).', 'Similar to the treatment of the pan-HDAC inhibitors, HDAC6 knockdown increased total tau, but not total tubulin (E,F).', 'Increase of tau acetylation by the inhibition of cytosolic HDAC6.']","Figure 3 Increase of tau acetylation by the inhibition of cytosolic HDAC6. ( ) Cellular localization of HDAC enzymes. A total of 11 HDACs belonging to three classes were mainly distributed in nucleus, cytosol, and both nucleus and cytosol (shuttle). ( ) mRNA expression levels of HDACs in siHDACs-transfected tau-BiFC cells. Tau-BiFC cells were transfected with HDAC3, HDAC5, or HDAC6 siRNA. After 72 h of transfection, total mRNA was extracted and then cDNA synthesized from total mRNA was analyzed by q-PCR with HDAC3, HDAC5, or HDAC6 specific primers. ( ) BiFC fluorescence images of tau-BiFC cells transfected with siRNA targeting HDAC3, HDAC5, or HDAC6. Nuclei were counterstained with Hoechst. Scale bar, 50 m. ( ) Quantification of BiFC fluorescence intensities. ( ) Immunoblot analysis of HDAC6, acetylated and total tubulin, and acetylated, phosphorylated, and total tau with anti-HDAC6, anti-ac--tubulin, anti-/-tubulin, anti-ac-Tau(K280), anti-p-Tau(S199), and Tau5 antibodies. Green arrows indicate Tau-VN173 and Tau-VC155. ( ) Quantification of HDAC6, acetylated and total tubulin, and acetylated, phosphorylated, and total tau levels. ( , , ) Data represent the mean S.D. of replicate experiments. * < 0.05. ** < 0.01, *** < 0.001.",yes
PMC7170022,Figure_3,oa_package/4e/61/PMC7170022.tar.gz,"['CECT showing the mass in the body and tail of the pancreas with solid areas alternating with cystic spaces (white arrows)CECT, contrast-enhanced computed tomographyPreoperatively, a mass with solid and cystic components was seen arising from the body and tail of the pancreas.']","Figure 3 CECT showing the mass in the body and tail of the pancreas with solid areas alternating with cystic spaces (white arrows) CECT, contrast-enhanced computed tomography",yes
PMC6754832,Figure_5,oa_package/72/97/PMC6754832.tar.gz,['The immunohistochemical study confirmed the angiosarcomatous nature of proliferation since there was a diffuse and intense positivity to anti-CD34 and anti-CD31 antibodies ().'],Figure 5 Microphotography showing intense positivity to anti-CD34 antibodies,yes
PMC6348748,Figure_6,oa_package/f3/68/PMC6348748.tar.gz,"['The IL10 RNA-interference to some extent attenuated inflammatory process in the lung tissue ( and ), and we observed mild alveolar and peribronchial inflammatory cellular infiltration as well as some reduction of thickness of interalveolar septa were shown.', ':Lung of the OVA-induced asthma + IL10 shRNA-interference rats.']","Figure 6: Lung of the OVA-induced asthma + IL10 shRNA-interference rats. Extravasated RBCs and cellular infiltrate in the alveolar spaces with dilatation and rupture of other alveoli is seen. Dilated and congested blood vessel is present. Alveoli (A), bronchioles (B), and blood vessels (bv), mononuclear cellular infiltration (I), extravasated RBCs (R) are indicated, 100.",yes
PMC6902725,Figure_6,oa_package/c3/15/PMC6902725.tar.gz,"['The up-regulation of GM-CSF was transient, and the levels equalized to those observed in control mice 24 h after AnxA1 treatment (A).', 'As shown in B, GM-CSF expression was clearly up-regulated in mFPR1-KO mice, suggesting that the up-regulation of lung GM-CSF is independent of mFPR1 activation.', 'AnxA1 induces differential pulmonary expression of the colony-stimulating factors via FPR2.', 'Treatment with WRW4 only had no effect on CSF expression levels in the lungs (C).', 'However, a combined treatment of AnxA1 and its antagonist WRW4 abrogated the effect of AnxA1 on GM-CSF up-regulation (C), indicating that GM-CSF expression was mediated via AnxA1-induced activation of mFPR2.', 'GM-CSF mRNA levels were significantly increased in primary lung cells but not in BAL cells, which are predominantly AMs, upon stimulation with AnxA1 for 8 h (D).']","Figure 6 AnxA1 induces differential pulmonary expression of the colony-stimulating factors FPR2. ) qPCR analysis of expression levels of GM-CSF, G-CSF, and M-CSF in lungs of AnxA1-treated mice compared with control animals at the indicated time points postinjection; 8 h, = 8; 24 h, 48 h, = 5 mice/group (Mann-Whitney test). ). AnxA1-induced changes in expression levels detected at 8 h postinjection in lungs of FPR1 KO mice. ) CSF expression in mice treated either with a single dose or a combination of AnxA1 and the FPR2 antagonist WRW4. Note that WRW4 abrogates the AnxA1-mediated effect on GM-CSF up-regulation. ) qPCR analysis of GM-CSF expression levels in primary lung cells and BAL fluid cells treated or not for 8 h with 400 nM AnxA1. For all graphs, bars represent fold change of relative expression levels and dashed (dotted) lines indicate no change (expression ratio = 1).",yes
PMC7585049,Figure_1,oa_package/97/53/PMC7585049.tar.gz,"['2%) with hand-sewn, of which eight were side-to-end (), four were end-to-end and one case with a colonic J pouch.', 'a) Residual inferior rectal cancer after neoadjuvant treatment.', 'Two cases (10.']",Fig. 1 a) Residual inferior rectal cancer after neoadjuvant treatment. b) Hand-sewn coloanal anastomosis.,yes
PMC6305895,Figure_5,oa_package/5b/b1/PMC6305895.tar.gz,['Arthroscopic photograph with the arthroscope inserted through the anterior portal with the patient in the beach-chair position.'],Fig 5 Arthroscopic photograph with the arthroscope inserted through the anterior portal with the patient in the beach-chair position. The arthroscope has been driven into the posterior paralabral cyst after the limited capsulectomy. This view shows the extensive nature of the cyst and confirms that adequate decompression has been performed.,yes
PMC9712688,Figure_6,oa_package/e7/8e/PMC9712688.tar.gz,"[' 6A, 4D and S9).', ' 6A).', ' 6A) but negative on the native livers (', ' 6B), suggesting HTLV-1 infection after surgery, probably donor-derived.', '6B).', 'HTLV-1 specific Tax protein detected on liver graft, native liver, and bone marrow samples from aGVHD patients by IMC.', 'DiscussionIn this study, we detected HTLV-1 cDNA and HTLV-1 specific Tax proteins from peripheral blood samples, fresh tissue sections, and formalin-fixed and paraffin-embedded (FFPE) tissue sections of all aGVHD-diagnosed patients by pre-synthesized TargetSeq, CyTOF, and IMC.']","Fig. 6 HTLV-1 specific Tax protein detected on liver graft, native liver, and bone marrow samples from aGVHD patients by IMC. Representative Tax images of two independently stained liver graft and native liver show two overlap patterns. Each row of figures display the same tissue type. Displayed channels for liver graft samples from left to right are: CD68 (green)/Tax (red)/EIF1AY (white)/DNA (blue), CD68 (green)/Tax (red)/DNA (blue), CD68 (green)/Tax (red)/EIF1AY (white)/DNA (blue). Displayed channels for native liver samples are CD68 (green)/Tax (red)/DNA (blue). Red arrows pinpoint the multi-signal overlap position. Representative Tax IMC images of two independently stained bone marrow and native liver sections show an overlap of Tax (red) and DNA (blue). Scale bar=50 or 100m, shown in the images, respectively.",yes
PMC8289960,Figure_3,oa_package/5a/fc/PMC8289960.tar.gz,"[' 3a i) in the blast-exposed ear did not change at any frequency region in any group (Supplemental ', 'Changes in the organ of Corti after shock-wave exposure.', 'Subsequently, we focused on the synapses between the IHCs and cochlear nerve fibers.', ' 3a f) in the shock tube and LISW groups decreased significantly compared to the control group at all frequencies tested (2-way ANOVA, F2, 72 = 315.', ' 3k).', ' 3g j).']","Figure 3 Changes in the organ of Corti after shock-wave exposure. ( ) Confocal image of immunohistochemical analysis of the organs of Corti ( , blue, anti-myosin 7a; red, CtBP2), enlarged image of the inner hair cells seen in the white square in ( , blue, anti-myosin 7a; red, anti-CtBP2; anti-CtBP2 also stains the IHC nuclei) at 16kHz. ( ) A typical example of synaptic complexes from the non-exposed ( , ) and shockwave-exposed ( , ) z-stacks, red, anti-CtBP2; green: anti-GluA2. Orphan ribbons, lacking apposed glutamate receptor patches, were observed in the blast-exposed ears ( ). The white arrowhead indicates orphan ribbons ( ). The quantification of the synaptic ribbons from a single IHC, observed 28days after exposure and in the controls. The number of synaptic ribbons decreased in both the shock-tube and LISW groups compared to the control group at all tested frequencies. The number of synaptic ribbons was significantly fewer in the shock-tube group at the lower frequencies (5.6 and 8.0kHz) and fewer in the LISW group at a higher frequency (32.0kHz): these trends were similar to the ABR threshold shift shown in Fig. g. ( ) The histograms compare the % of orphan ribbons per single IHC at the frequencies at which the ABR and DPOAE were measured, as compiled for all control and exposed (shock tube and LISW) cases. All data were gathered 28days post-blast exposure. The asterisks indicate significant differences ( <0.05) (2-way ANOVA, followed by Bonferroni correction for multiple comparisons). The error bars indicate the SEs of the means (n=5 in each group). The scale bar represents 5m. The scale bar in panel b, which represents 50m, is applicable for panels . The scale bar in panel , which is 5m, is applicable from panels , and . The scale bar in panel , which represents 0.5m, applies from panels . ABR, auditory brainstem response; DPOAE, distortion product otoacoustic emission; IHC, inner hair cell; LISW, laser-induced shock wave; OHC, outer hair cell.",yes
PMC6842214,Figure_5,oa_package/ef/7a/PMC6842214.tar.gz,"['5).', 'Disorganisation of axonal MTs upon loss of different MT regulators in Drosophila primary neurons.', ""Scale bar in A represents 10 m for the two neurons and 4 m in close-upsAs an attempt to explain the occurrence of this MT phenotype across mutant conditions and animal groups, we developed the model of 'local axon homeostasis' [37, 89], based on two fundamental elements:(1) The model proposes that MTs in axons show a strong bias to become disorganised and curl up."", '5; [87, 88]) - and below 1 m MTs are believed to break [128, 129].', '5).', '5e).', '5B [75]).', '5e) - and the same is true for functional loss of its two mammalian homologues ACF7 and dystonin in culture and in vivo [57, 59, 87, 90].', '5d [74]).', '5b, e) does not occur when removing actin from axon shafts [21, 75, 77].']","Fig. 5 Disorganisation of axonal MTs upon loss of different MT regulators in primary neurons. Normal neuron (wild-type, wt) with soma (asterisk), axon shaft (curved arrow) and growth cone (tip of most distal MT indicated by arrow head). mutant neuron where the area of MT disorganisation is framed by a red stippled box and shown as close-up on the right. Similar close-ups shown for , and mutant neurons. Note that the four mutated factors perform fundamentally different molecular functions, with Eb1 being a MT plus-end binder ('8' in Fig. ), Efa6 a cortical collapse factor ('4' in Fig. ), Khc a kinesin-1 motor protein ('A-E' in Fig. ) and Shot a multi-functional cross-linker ('1-3, 5, 11' in Fig. ). All neurons were derived from wild-type or homozygous mutant embryos, mechanically and chemically dissociated, kept for 7 days in pre-culture in a centrifuge tube to deplete any maternal gene product, mechanically and chemically dissociated again, cultured on concanavalin A-coated glass coverslips for 1day at 21C, fixed and stained with anti--tubulin (DM1A, Sigma; procedures detailed elsewhere: [ ]); images were taken by A.V. using STED (stimulated emission depletion) microscopy. Scale bar in A represents 10 m for the two neurons and 4 m in close-ups",yes
PMC11407995,Figure_5,oa_package/61/79/PMC11407995.tar.gz,"['Over two years, the lesion increased in size, so we integrated the diagnostic process with mammography that showed a radiopaque lesion with clear-cut margins of 22 mm in the upper-outer quadrant of right breast ().', 'Mammography: cranio-caudal view showing a radiopaque lesion with clear-cut margins of 22 mm in the upper-outer quadrant of right breast.', 'Subsequently, we performed breast surgery with the excision of the lesion and the histological examination confirmed the diagnosis of dermatofibrosarcoma.']",Fig. 5 Mammography: cranio-caudal view showing a radiopaque lesion with clear-cut margins of 22mm in the upper-outer quadrant of right breast.,yes
PMC7422076,Figure_7,oa_package/67/e6/PMC7422076.tar.gz,"['After verification with DSA, the vessel was treated with coil embolization (s 7(a) and 7(b)) thrombosing the PSA.', '006""/>(a, b) Pre- and postembolization of the final inferior PDA feeding the bilobed PSA (arrowheads) which finally led to complete thrombosis.']","Figure 7 (a, b) Pre- and postembolization of the final inferior PDA feeding the bilobed PSA (arrowheads) which finally led to complete thrombosis.",yes
PMC10693286,Figure_5,oa_package/dd/4b/PMC10693286.tar.gz,"[' Histopathological slide of the leiomyoma The arrow depicts the spindle-shaped smooth muscle cellsThe intra-operative blood loss was around 100-120 milliliters, which is permissible given the anemic condition of the woman.']",Figure 5 Histopathological slide of the leiomyoma The arrow depicts the spindle-shaped smooth muscle cells,yes
PMC5709977,Figure_4,oa_package/34/b0/PMC5709977.tar.gz,"[' 4, Additional file 5: S2a).', ' 4 and Additional file 5: S2b, remarkably higher APP-CTF protein levels were detected in fAD and sAD neurons.', 'Characterization of amyloid precursor protein (APP) and amyloid precursor protein carboxy-terminal fragment (APP-CTF) expression during neuronal differentiation.', '05)\nEpitope-specific TAU hyperphosphorylation was detected in fAD and sAD linesOne of the proposed mechanisms for the TAU protein pathomechanism in AD are posttranslational modifications through abnormal phosphorylation (hyperphosphorylation) (reviewed in [55]).']","Fig. 4 Characterization of amyloid precursor protein (APP) and amyloid precursor protein carboxy-terminal fragment (APP-CTF) expression during neuronal differentiation. Representative immunoblots of ( ) APP and APP-CTF in control neurons (ctrl-1), early-onset familial Alzheimers disease (fAD)-derived neurons (fAD-1fAD-4), and ( ) sporadic Alzheimers disease (sAD)-derived neurons (sAD-1sAD-6) at terminal differentiation day 14 (TD14), TD28, TD42, TD56, and TD70. Quantification of ( ) APP and ( ) APP-CTF signals at TD70 was normalized to . Data are presented as meanSEM ( =3). Dunnetts test was performed to evaluate the significance of groups compared with control (* <0.05)",yes
PMC6752298,Figure_1,oa_package/28/ba/PMC6752298.tar.gz,"[' 1a,b).', ' 1c,d), which may underlie its agonistic action\nmechanism.', 'Volatile anesthetics activate TRPV1 channels heterologously\nexpressed in HEK-293 cells and in flexor digitorum brevis (FDB)\nisolated fibers.', '0001TRPV1 is a SR Ca2+ channel activated by VAs in\nskeletal muscle cellsWe have previously shown that Trpv1 is a functional SR\nCa2+-leak channel in adult mouse skeletal muscle\ncells.', ' 1e shows that Fluo-4-loaded cells undergo massive\nincrease of cytosolic [Ca2+] after isoflurane\nperfusion (median of max = 1.', ' 1e,f).', ' 1g).', ' 1h).']","Fig. 1 Volatile anesthetics activate TRPV1 channels heterologouslyexpressed in HEK-293 cells and in flexor digitorum brevis (FDB)isolated fibers. ( , ) Original recordings of thebaseline TRPV1 current (I ) (acquiredat point 1 of the time course in ( )) and isoflurane-activated (0.125mM)(acquired at point 2 of the time course) in response to pulseprotocol with voltage-ramp portion shown above the recordings.Isoflurane activated a membrane current with biophysicalproperties, such as a prominent outward rectification and closeto 0mV reversal potential, which is similar to the currentactivated by the established TRPV1 stimulus, capsaicin (data notshown) ( =7). ( ) Examples of the baseline andhalothane-activated I in response tothe depicted voltage-clamp protocol, which were used to measurevoltage dependence of TRPV1 channel open probability ( in( ); experiments wereperformed at 20C); arrows in ( ) point to the I tail currents at +60mV. Tail currents were measured during thefirst millisecond of the final step +60mV and normalized to themaximal tail current. Normalized amplitude as a function ofconditioning depolarizing pulse (ranging from 120 to +160mV)corresponds to the apparent (meanS.E.M., =3). Membrane currentswere recorded in the whole cell configuration using the Axopatch200B amplifier (Molecular Devices, Union City, CA).Extracellular solution containing (in mM): 150 NaCl, 1MgCl , 5 glucose, 10 HEPES, pH 7.3.Intracellular solution containing (in mM): 150 NaCl, 3MgCl , 5 EGTA, 10 HEPES, pH 7.3.( ) Traces showrepresentative curve obtained after stimulation of single fiberswith isoflurane (6mM; black line) or in presence of capsazepine(CPZ; 100M; light gray line). ( ) Changes in fluorescence ratio F/F0(peak-resting) induced by drugs as indicated in table abovegraphs. Capsazepine was added 25min prior to isoflurane.Corresponding scatterplots of max expressed as median; dataare from 16 cells (without CPZ) and 6 cells (with CPZ) from atleast 4 independent fibers preparations. Changes in fluorescenceratio F/F0 (peak-resting) induced by ( ) capsaicin (100M) or by ( ) isoflurane (0.5mM; 1mM; 6mM) inC57Bl6J (black) or mice (red).Corresponding scatterplots of max value expressed as median; forthe capsaicin response, data are from 42 cells (wild-type, WT),7 cells (WT+CPZ), and 10 cells ( ) from atleast 4 independent fibers preparations. For the isofluraneresponse, data are from 6 and 5 cells (0.5mM isoflurane, WT andTRPV1 respectively); 9 and 8cells (1mM isoflurane, WT andTRPV1 respectively); and 12and 10 cells (6mM isoflurane, WT andTRPV1 respectively) from atleast 4 independent fiber preparations. MannWhitney tests wereused: * <0.05,** <0.01,*** <0.001,**** <0.0001",yes
PMC10629324,Figure_7,oa_package/a0/9a/PMC10629324.tar.gz,"['We performed embolization using PVa 355-500 mm in the ECA branch to the occipital and superficial right temporalis arteries [].', ':(a) Pre Embolization and (b) Post embolization intra-arterial embolization were done at the maxillary artery, middle deep temporal branch, meningeal accessories artery, and occipital artery use 250 micron 355 microparticle.']","Figure 7: (a) Pre Embolization and (b) Post embolization intra-arterial embolization were done at the maxillary artery, middle deep temporal branch, meningeal accessories artery, and occipital artery use 250 micron355 microparticle.",yes
PMC8691626,Figure_7,oa_package/1f/85/PMC8691626.tar.gz,"['Control neutrophils typically form NETs from 2 hours after stimulation, but up until 3 hours of incubation with PMA only very few PLS neutrophils from family A had produced NETs as visualized microscopically (A).', 'The Sytox green assay (B) confirmed this observation, showing that PMA-triggered NET formation was virtually absent 3 h.', 'PLS+PMA, 3h, n = 2) more NETs than unstimulated PLS cells (B).', 'g007NETosis of neutrophils upon PMA stimulation.']",10.1371/journal.pone.0261724.g007,yes
PMC10413489,Figure_1,oa_package/c3/a7/PMC10413489.tar.gz,"['RPOC, retained products of conception\n 1 reveals an ultrasound scan with a 7 mm postpartum RPOC in an asymptomatic patient, with a positive pathological result confirming the presence of intrauterine residual remnant.', '\nSonographic scan for RPOC with positive pathology result.', 'An ultrasound scan which reveals a 7 mm postpartum RPOC in an asymptomatic patient, with a positive pathological result confirming the presence of intrauterine residual remnant\nWe evaluated the PPV and NPV for the maximum size of each dimension measured on ultrasound scan for predicting positive pathology results for RPOC (Table 3).']","Fig. 1 Sonographic scan for RPOC with positive pathology result. An ultrasound scan which reveals a 7mm postpartum RPOC in an asymptomatic patient, with a positive pathological result confirming the presence of intrauterine residual remnant",yes
PMC2919420,Figure_5,oa_package/7f/4c/PMC2919420.tar.gz,"['The pattern of insoluble proteins in young-adult animals subjected to daf-2 RNAi (from the first larval stage) resembled that of wild type (A).', 'Similarly, Western blot analysis showed that daf-2 RNAi treatment greatly reduced the extent of both RHO-1 and DAF-21 insolubility with age and moderately reduced the extent of KIN-19 and PAR-5 age-dependent insolubility (B).', 'g005Reduced insulin/IGF-1-like signaling protects against age-dependent protein insolubility and aggregation.', '4-fold) (C).', 'Moreover, the puncta in the daf-2( ) mutant tended to contain mobile KIN-19 (even in very old Day 38 animals) (E and Table 1).']",10.1371/journal.pbio.1000450.g005,yes
PMC10700051,Figure_7,oa_package/ec/cd/PMC10700051.tar.gz,['Temporization of the prepared posterior teeth.'],Figure 7 Temporization of the prepared posterior teeth.,yes
PMC8675570,Figure_3,oa_package/a1/5b/PMC8675570.tar.gz,[],Figure 3 Transverse view: Right ureterovesical junction stone,yes
PMC6188173,Figure_5,oa_package/5f/18/PMC6188173.tar.gz,['Parasagittal MRI postsurgical resection.'],"Figure 5 Parasagittal MRI postsurgical resection. T1 weighted enhanced MRIof the cervical spine four weeks after resection, showing gross total resection with associated postoperative changes in the parasagittal plane.",yes
PMC10749789,Figure_5,oa_package/3f/49/PMC10749789.tar.gz,"['We used the spatial transcriptomics annotations generated by pathologists as the gold standard to assess the gene expression predicted by THItoGene, HisToGene and His2ST using K-means clustering ().', 'The spatial clustering analysis compares gene expression predictions from three different methods: THItoGene, Hist2ST and HisTGene.']","Figure 5 The spatial clustering analysis compares gene expression predictions from three different methods: THItoGene, Hist2ST and HisTGene. Histological images annotated by the pathologist are displayed in the first column. The second column presents the observed gene expression clustering results, while the last three columns show the clustering outcomes for gene expression as predicted by different methods.",yes
PMC3223696,Figure_1,oa_package/b4/af/PMC3223696.tar.gz,"['Proteolytic Cleavage of HTTHTT is susceptible to proteolysis by a number of proteases ().', 'The modulation of autophagy is therapeutically promising in HD: the inhibition of TOR by rapamycin enhances the clearance of mutant HTT-containing aggregates via the autophagy-lysosome pathway ().', '38 As a result of extensive mitochondrial depolarization, neurons exposed to prolonged Ca2 rise become vulnerable to excitotoxic insults ().', '40, 103, 104 In HD, mutant HTT affects glutamatergic signals as a result of altered neurotransmitter release and activity of the glutamate-ionotropic receptors at the plasma membrane ().', ""elegans mutant that lives twice as long as wild typeNature19933664614648247153MorleyJFBrignullHRWeyersJJMorimotoRIThe threshold for polyglutamine-expansion protein aggregation and cellular toxicity is dynamic and influenced by aging in CaenorhabditiselegansProc Natl Acad Sci USA2002991041710422HsuALMurphyCTKenyonCRegulation of aging and age-related disease by DAF-16 and heat-shock factorScience20033001142114512750521PouladiMAXieYSkotteNHEhrnhoeferDEGrahamRKKimJEFull-length huntingtin levels modulate body weight by influencing insulin-like growth factor 1 expressionHum Mol Genet2010191528153820097678MitchellGCFillingerJLSittadjodySAvilaJLBurdRLimesandKHIGF1 activates cell cycle arrest following irradiation by reducing binding of DeltaNp63 to the p21 promoterCell Death Dis20101e5021480565van HamTJHolmbergMAvan der GootATTeulingEGarcia-ArencibiaMKimHEIdentification of MOAG-4/SERF as a regulator of age-related proteotoxicityCell201014260161220723760GiacomelloMDragoIPizzoPPozzanTMitochondrial Ca2 as a key regulator of cell life and deathCell Death Differ2007141267127417431419LiZOkamotoKHayashiYShengMThe importance of dendritic mitochondria in the morphogenesis and plasticity of spines and synapsesCell200411987388715607982YoungKWBamptonETPinonLBanoDNicoteraPMitochondrial Ca2 signalling in hippocampal neuronsCell Calcium20084329630617764739CostaVGiacomelloMHudecRLopreiatoRErmakGLimDMitochondrial fission and cristae disruption increase the response of cell models of Huntington's disease to apoptotic stimuliEMBO Mol Med2010249050321069748SongWChenJPetrilliALiotGKlinglmayrEZhouYMutant huntingtin binds the mitochondrial fission GTPase dynamin-related protein-1 and increases its enzymatic activityNat Med20111737738221336284SassoneJColciagoCMarchiPAscardiCAlbertiLDi PardoAMutant Huntingtin induces activation of the Bcl-2/adenovirus E1B 19-kDa interacting protein (BNip3)Cell Death Dis20101e721364626BanoDNicoteraPCa2 signals and neuronal death in brain ischemiaStroke200738(2 Suppl67467617261713AnkarcronaMDypbuktJMBonfocoEZhivotovskyBOrreniusSLiptonSAGlutamate-induced neuronal death: a succession of necrosis or apoptosis depending on mitochondrial functionNeuron1995159619737576644CuiLJeongHBoroveckiFParkhurstCNTaneseNKraincDTranscriptional repression of PGC-1alpha by mutant huntingtin leads to mitochondrial dysfunction and neurodegenerationCell2006127596917018277ZhuSZhangYBaiGLiHNecrostatin-1 ameliorates symptoms in R6/2 transgenic mouse model of Huntington's diseaseCell Death Dis20112e11521359116ZhouRYazdiASMenuPTschoppJA role for mitochondria in NLRP3 inflammasome activationNature201146922122521124315RuizAMatuteCAlberdiEIntracellular Ca2 release through ryanodine receptors contributes to AMPA receptor-mediated mitochondrial dysfunction and ER stress in oligodendrocytesCell Death Dis20101e5421364659GlassCKSaijoKWinnerBMarchettoMCGageFHMechanisms underlying inflammation in neurodegenerationCell201014091893420303880SappEKegelKBAroninNHashikawaTUchiyamaYTohyamaKEarly and progressive accumulation of reactive microglia in the Huntington disease brainJ Neuropathol Exp Neurol20016016117211273004RodriguezJJWittonJOlabarriaMNoristaniHNVerkhratskyAIncrease in the density of resting microglia precedes neuritic plaque formation and microglial activation in a transgenic model of Alzheimer's diseaseCell Death Dis20101e121364611ShinJYFangZHYuZXWangCELiSHLiXJExpression of mutant huntingtin in glial cells contributes to neuronal excitotoxicityJ Cell Biol20051711001101216365166ChouSYWengJYLaiHLLiaoFSunSHTuPHExpanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytesJ Neurosci2008283277329018367595NicolaiJBurbassiSRubinJMeucciOCXCL12 inhibits expression of the NMDA receptor's NR2B subunit through a histone deacetylase-dependent pathway contributing to neuronal survivalCell Death Dis20101e3321364640YongVWRivestSTaking advantage of the systemic immune system to cure brain diseasesNeuron200964556019840549Representative intracellular events in neurons expressing mutant HTT.""]","Figure 1 Representative intracellular events in neurons expressing mutant HTT. In HD, processing of mutant HTT by caspases, calpains and MMPs facilitates the formation of intracellular aggregates, which are mainly degraded by autophagy. Failure in the clearance of HTT proteolytic fragments eventually results in excessive cytosolic Ca concentration and organelle dysfunctions",yes
PMC10407070,Figure_2,oa_package/ca/75/PMC10407070.tar.gz,"[' ().', 'A 43-year-old male showing a shattered spleen after a car accident.']","Fig. 2 A 43-year-old male showing a shattered spleen after a car accident. CT shows high-grade splenic laceration with contrast extravasation (arrow). Splenic angiography confirms a shattered spleen with multifocal contrast leakage and pseudoaneurysms. Residual normal parenchymal enhancement was not detected on splenic angiogram; thus, embolization of the main splenic artery was done using an N-butyl-2-cyanoacrylate and lipiodol mixture. A follow-up angiogram after embolization reveals no residual bleeding but shows patent pancreatic arteries arising from the proximal portion of the splenic artery (arrows). CT after two months shows viable splenic parenchyma after embolization of the main splenic artery (arrow).",yes
PMC4151867,Figure_2,oa_package/b9/91/PMC4151867.tar.gz,"['Benazepril affects the TGF- 1, ILK and -SMA expression in renal tissue and glomeruli.']","Figure 2 Immunohistochemistry staining of TGF- in the rat kidneys of the NC, DN and ACEI groups. Immunofluorescence staining of ILK in rat kidneys of NC, DN and ACEI group. There are significant increases in ILK expression in DN rat compared with control mice. Rabbit IgG was used as a negative control and shown in the right corner of each figure. Immunofluorescence staining of -SMA in the rat kidneys of the different groups. There are significant increases in -SMA expression in DN rat compared with control mice. Mouse IgG was used as a negative control and is shown in the right corner of each figure. Bars=50m.",yes
PMC4471193,Figure_2,oa_package/a3/26/PMC4471193.tar.gz,"['7 cm) (A).', '2 mg / g) (B).', '03) compared to the untreated group (B).', '3 cm) (A) or relative colon weight (8.', '5 mg / g) (B).', 'g002Effect of fucoidan extracts on colon and spleen.']",10.1371/journal.pone.0128453.g002,yes
PMC7710717,Figure_3,oa_package/4f/d3/PMC7710717.tar.gz,"['3a).', '3b).', '3c), whereas silencing HOXA-AS2 resulted in increased expression of CD11b and CD14 (', '3d).', '3e).', '3f).', 'Effect of HOXA-AS2 on AML cells differentiation.', 'HOXA-AS2 regulated LATS2 transcription in AML CellsIn order to study the role of LATS2 in AML, we next explored the relationship between HOXA-AS2 and LATS2.']","Fig. 3 Effect of HOXA-AS2 on AML cells differentiation. , The differentiation ability of NB4 and THP-1 cells transfected with HOXA-AS2 plasmid or transfected with si-HOXA-AS2#1, si-HOXA-AS2#2 was determined by flow-cytometry analysis. , The levels of CD11b and CD14 protein in NB4 and THP-1 cells transfected with HOXA-AS2 plasmid or with si-HOXA-AS2#1 and si-HOXA-AS2#2 were detected by western blotting assays. The differentiation ability of NB4 and THP-1 cells transfected with si-HOXA-AS2#1, si-HOXA-AS2#2 was assessed by evaluating NBT reduction ability. The CD11b and CD14 expression of tumors from sh-HOXA-AS2 and sh-NC groups were determined by immunohistochemical staining. * <0.05, ** <0.01 versus control groups.",yes
PMC8544166,Figure_18,oa_package/e3/f3/PMC8544166.tar.gz,[],"Figure 18. Submandibular skin (mental) gland, H&E.",yes
PMC8891206,Figure_8,oa_package/04/96/PMC8891206.tar.gz,"[' 8).', 'Idiopathic juvenile osteoporosis,Bruck syndrome type 1 and type 2a A stillborn with osteogenesis imperfecta.', 'Radiographs show multiple Wormian bones (arrow) due to defective calvarial ossification and generalized osteoporosis with a healing fracture of the right femoral shaft and a healed fracture of the left femoral shaftThe differential diagnosis between non-accidental injury and mild osteogenesis imperfecta can be challenging.']","Fig. 8 A stillborn with osteogenesis imperfecta. Radiograph shows a beaded appearance of the ribs and an accordion-like wavy appearance of the long bones as a consequence of in utero multiple fractures. , A 2-year-old patient with osteogenesis imperfecta. Radiographs show multiple Wormian bones (arrow) due to defective calvarial ossification and generalized osteoporosis with a healing fracture of the right femoral shaft and a healed fracture of the left femoral shaft",yes
PMC10546696,Figure_3,oa_package/28/f0/PMC10546696.tar.gz,"[' 3A).', ' 3B and C).', ' 3C and D, arrow in each figure).', 'Abdominal and pelvic CT findings.', 'The solid nodular mass showed a heterogeneous rim-like enhancement pattern with attenuated central areas, unlike the other cystic or solid compartments of the tumorOpen laparotomy was conducted and revealed a multicystic exophytic tumor measuring 11.']","Fig. 3 Abdominal and pelvic CT findings. Coronal image. The multicystic mass changed in position to the caudal direction in comparison with the MRI findings, and a jejunal origin was suggested. No dilatation of the intestine or intraluminal component was found. Plain image of the tumor. Contrast-enhanced image of the tumor. The multicystic tumor showed a thin wall and multiple septations, with low-density liquid showing no enhancement and an enhanced solid compartment. The thickened portion of the left ventral region is the jejunum. On the back side of the solid compartment, there was a round solid nodular mass, measuring 18mm in maximal diameter, showing strong enhancement in the early period of the contrast-enhanced sequence, unlike the cystic tumor component. Sagittal contrast-enhanced CT image. The solid nodular mass showed a heterogeneous rim-like enhancement pattern with attenuated central areas, unlike the other cystic or solid compartments of the tumor",yes
PMC6422394,Figure_5,oa_package/8f/3c/PMC6422394.tar.gz,"['Western blot analysis of protease resistant PrP and PrP gene analysisWestern blot analysis indicated the presence of type 1 PrPSc, with a relative molecular mass of the unglycosylated band of approximately 21 kDa ().', '1545525-F0005.']",10.1080/19336896.2018.1545525-F0005,yes
PMC1860060,Figure_1,oa_package/e4/df/PMC1860060.tar.gz,['1186/ar1169s and TablesSchematic diagram of the putative interactions of pathogenic Th17 cells in the synovial microenvironment.'],"Figure 1 Schematic diagram of the putative interactions of pathogenic Th17 cells in the synovial microenvironment. Induction of T-cell responses in rheumatoid arthritis (RA) is initiated by T-cell receptor (TCR) interaction with shared epitope major histocompatibility complex class II (MHCII-SE) and peptide on antigen-presenting cells (APCs) either systemically or in the synovium. Accessory molecules expressed by APCs, including ICAM-1 (intercellular adhesion molecule-1) (CD54), OX40L (CD252), inducible costimulator (ICOS) ligand (CD275), B7-1 (CD80), and B7-2 (CD86), participate in T-cell activation by binding lymphocyte function-associated antigen (LFA)-1 (CD11a/CD18), OX40 (CD134), ICOS (CD278), and CD28. Activated fibroblast-like synoviocytes (FLS) may also participate in antigen presentation and have additional accessory molecules such as LFA-3 (CD58) and ALCAM (activated leukocyte cell adhesion molecule) (CD166) which interact with T cell-expressed CD2 and CD6, respectively. Cytokines interleukin (IL)-6 and transforming growth factor-beta (TGF-), most likely derived from activated APCs, signal the T cell to differentiate into IL-17-producing Th17 cells. IL-17 has independent and synergistic effects with other proinflammatory cytokines (tumor necrosis factor-alpha [TNF-] and IL-1) in the synovium to induce further cytokine release, matrix metalloproteinase production, RANK/RANK ligand (CD265/CD254) expression, and osteoclastogenesis. CD40L (CD154) interaction with CD40 also leads to activation of synovial monocytes/macrophages (Mo/Mac), FLS, and B cells. Although present in the synovia of most patients with RA, CD4 CD25 regulatory T (Treg) cells are ineffective at controlling inflammation and may be deactivated by synovial TNF-. IL-10 is abundant in synovial fluid but its effect on Th17 regulation has yet to be determined. Expression of accessory molecules on Th17 cells, as denoted in the figure, are speculative and are inferred from expressions found on non-subdivided T-cell populations in animal models. Further investigation is necessary to directly demonstrate expression of these structures on the Th17 cell subset in human RA synovium. DC, dendritic cell; RANK, receptor activator of nuclear factor-kappa B.",yes
PMC8929144,Figure_2,oa_package/17/28/PMC8929144.tar.gz,"['SARS-CoV-2 did not result in a productive infection of primary human monocytes as indicated by a lack of an increase in genomic RNA levels (A).', 'However, SARS-CoV-2 infection resulted in increased SIRP levels in primary monocytes (B, s S4 and S5).', '1)-infected Caco2 cells as determined by flow cytometry; S4: Uncropped Western blots to ; S5: Quantification of SIRP levels in SARS-CoV-2-infected primary human monocytes; Table S1: Literature search for CD47 aging .', 'SARS-CoV-2 infection increases SIRP in primary human monocytes.']","Figure 2 SARS-CoV-2 infection increases SIRP in primary human monocytes. ( ) SARS-CoV-2. The (MOI 1) infection of primary human monocytes does not result in the production of genomic viral RNA, as detected by PCR. ( ) SARS-CoV-2 strain FFM7 (MOI 1)-infected primary human monocytes display enhanced SIRP levels. Uncropped blots are provided in . Quantification of the protein levels is provided in .",yes
PMC10769877,Figure_10,oa_package/1b/1d/PMC10769877.tar.gz,[],"Extended Data Fig. 4 UMAP showing the expression of genes used to identify the different cell population clusters in the scRNA-seq dataset. Expression levels of cell type defining markers are shown as blue dots overlapping the original UMAP used to determine the clusters of cells (n=5, each group; samples pooled from one experiment).",yes
PMC9870076,Figure_5,oa_package/7e/a0/PMC9870076.tar.gz,"['Analysis of circulating autologous mouse anti-sheep antibodies revealed similar levels of total IgG, as well as IgG subclass antibodies, ruling out a B cell mediated effect of GC treatment (Supplemental ).', 'On day 10 of cGN, the chemokine receptor CXCR3 was highly expressed on renal CD4+ T cells, followed by CCR5, and both are markers of Th1 cells (A and Supplemental ).', 'This result correlates with the finding that the 2 main renal CD4+ T cell clusters identified by scRNA-Seq differed in their relative chemokine receptor mRNA expression in a manner allowing the assumption to be made that the predominantly occurring CXCR3-expressing memory CD4+ Th1 cells fail to infiltrate the kidney after high-dose steroid treatment (B).', 'Likewise, in the experimental setting of a short high-dose steroid treatment regimen (C), we found that IFN- producing CD4+ T cells were predominantly reduced in kidneys of nephritic steroid treated mice, while the numbers of IL-17 producing CD4 + T cells and Tregs were unchanged (D and Supplemental , A and B).', 'In addition, the corresponding Th1-associated cytokine and chemokine mRNA levels, most importantly Cxcl9 and Cxcl10, were specifically and significantly downregulated after steroid treatment, whereas other targets such as Il-17a and Ccl2 mainly remained unchanged (E and Supplemental C).', 'Next, we performed in situ hybridization analyses (RNAscope) to localize the renal production of the CXCR3-attracting chemokines CXCL9 and CXCL10, and this production was primarily detectable in cells corresponding to the proximal tubular epithelium in the periglomerular and tubulointerstitial spaces (F).', 'In support of this finding, in vitro experiments showed that the coapplication of steroids abolished, in a dose-dependent manner, the cytokine-induced production of CXCL9 and CXCL10 by proximal tubular epithelial cells (pTECs), further corroborating the role of steroids in changing the local proinflammatory environment as a means of regulating the renal T cell infiltrate (G and Supplemental 0).', 'According to the previously used experimental setup, we then treated them with PBS or steroids 2 days before sacrificing them for analyses (H).', 'Flow cytometric analyses of renal CD4+ T cells revealed comparable numbers of cells in PBS- and steroid-treated animals and similar numbers of CD3+ T cells as a result of the quantification of IHC CD3 staining of kidney sections from the groups mentioned above (, I and J).', 'The recruitment of CXCR3+ Th1 cells in murine cGN is attenuated by glucocorticoids through the dampened production of the corresponding chemokines by tubular epithelial cells.']","Figure 5 The recruitment of CXCR3 Th1 cells in murine cGN is attenuated by glucocorticoids through the dampened production of the corresponding chemokines by tubular epithelial cells. ( ) Representative plots showing mean percentages of CD4 and CD8 T cells, respectively, and percentage CXCR3 positivity of CD4 T cells, as well as relative chemokine receptor expression of CD3 CD4 T cells obtained from nephritic animals at day 10 of cGN determined by flow cytometric analyses. ( ) Analysis of the single-cell RNA-Seq data of renal CD3 T cells of nephritic mice analyzed for relative chemokine receptor expression of the CD4_naive and CD4_memory clusters. ( and ) Schematic representation of the experimental setup, and quantification of flow cytometric analyses of absolute numbers of CD3 CD4 IFN- T cells isolated from kidneys of untreated and steroid-treated nephritic mice. ( ) Reverse transcription PCR (RT-PCR) analyses of indicated mRNA expression from whole renal cortices of kidneys collected from the groups mentioned before. ( ) RNA scope analyses show the preferential localization of and mRNA to the tubulointerstitial compartment and markedly reduced expression in steroid treated mice. Scale of images is indicated by 1 cm in width equaling 15 m. ( ) Heatmap of chemokine protein levels in supernatants of proximal tubular epithelial cells after stimulation with either medium alone, IL-17A, IFN-, or TNF- under increasing concentrations of prednisolone. ( and ) Schematic representation of the experimental setup, and numbers of recovered renal CD4 T cells from PBS-treated and steroid- pulsed nephritic Rag1 mice after eleven days following i.v. transfer of CXCR3 CD4 T cells into both groups. ( ) Semiquantitative analysis of intrarenal T cell numbers derived from kidney sections IHC stained for CD3 for the groups mentioned before. Symbols represent individual data points, with the mean as a bar graph. Data were analyzed using a 2-tailed test. * < 0.05, ** < 0.01.",yes
PMC8611162,Figure_3,oa_package/e1/23/PMC8611162.tar.gz,[],Fig. 3 Tumoral MF mimicking angular cheilitis in a 73-year-old man,yes
PMC10070111,Figure_8,oa_package/ce/94/PMC10070111.tar.gz,"['We established a keloid implantation model in BALB/c nude mice using fresh keloid tissues to assess the effect of p-STAT6 inhibition on keloid progression in vivo (A).', 'Importantly, keloid implantations revealed uniform pathological characteristics similar to that of patient keloids confirmed by H E, Masson s trichrome staining, and CD163 IHC staining (B).', 'Moreover, several papillary dermis cells underwent apoptosis, while only a few reticular fibroblasts underwent apoptosis, in line with what is seen in patients with keloids (Supplemental ).', '05), while no significant change was observed in the control group (C).', '0001), indicating that suppression of p-STAT6 inhibited proliferation of KFs in vivo (D).', 'H E staining showed higher immune cell aggregation in the AS1517499 group, consistent with an observed increase in local tissue necrosis (E).', 'In addition, TUNEL staining showed that AS1517499 induced more fibroblast apoptosis in the whole layer of keloid-implanted dermis (F).', 'Moreover, AS1517499 treatment inhibited M2 macrophages, with CD163 staining virtually negative (, G and I).', 'IHC staining of -SMA showed that AS1517499 treatment reduced the intensity and positivity rate of -SMA, indicating a potent antimyofibroblast effect of p-STAT6 inhibition with AS1517499 treatment (, H and I).', 'AS1517499 inhibited fibrosis in keloid implantation model.']","Figure 8 AS1517499 inhibited fibrosis in keloid implantation model. ( ) Schematic diagram of animal experiment. ( ) Representative images of H&E, Massons trichrome, and IHC staining for CD163, CD31, -SMA, and Ki67 on day 0. Scale bars: 50 m. ( ) Images of transplantations after 10-day treatments. Quantification of volumes of xenografts in AS1517499-treated and control group is shown on the right (after dissection) and below (in vivo). ( ) Representative images of IHC staining of Ki67 in AS1517499-treated and control group. Quantification of the percentage of Ki67 cells is shown on the right. Scale bar: 100 m. ( ) Representative images of H&E staining of xenograft tissues in AS1517499-treated and control group. Scale bars: 50 m. ( ) Representative images of whole-slide scan of TUNEL staining in AS1517499-treated and control group. Cell nuclei were stained with DAPI (blue), and TUNEL-positive nuclei are stained in green. Scale bars: 100 m. ( and ) Representative IHC staining of CD163 and -SMA in AS1517499-treated and control group. Scale bars: 50 m. ( ) Quantification of percentage of CD163 and -SMA staining intensity. Error bars represent SD. * < 0.05; ** < 0.01; **** < 0.0001 by 2-tailed Students test. NS, not significant ( > 0.05).",yes
PMC8404898,Figure_8,oa_package/6b/07/PMC8404898.tar.gz,"['1 (614G) 21 days later (A); another group of hamsters (Table 1) was infected with B.', '427 21 days later (B).', 'Viral titer and vRNA levels in lungs were determined for all groups and timepoints ().', 'All animals had high levels of sgRNA and infectious virus in lungs at day 2 PI, but no infectious virus and only very low levels of sgRNA were detected in animals at day 21 PI (), confirming that the animals had been infected by the initial inoculum and then cleared the infection.', 'Two days after re-challenge (day 23 PI), we could not isolate virus or detect sgRNA in the lungs of any of the animals ().', '457626v1-f0007"" position=""float""/>.']",Figure 8. Prior infection with B.1 (614G) protects hamsters from subsequent challenge with B.1.427 or B.1.429. gRNA levels and infectious virus titer in hamster infected with B.1 (614G) and then challenged 21 days later with homologous B.1 (614G). sgRNA levels and infectious virus titer in hamster infected with B.1.427 and then challenged 21 days later with homologous B.1.427. sgRNA levels and infectious virus titer in hamster infected with B.1 (614G) and then challenged 21 days later with heterologous B.1.429. sgRNA levels and infectious virus titer in hamster infected with B.1 (614G) and then challenged 21 days later with heterologous B.1.427.,yes
PMC7436641,Figure_4,oa_package/01/91/PMC7436641.tar.gz,[],Figure 4 repair of rectal mucosa,yes
PMC10322670,Figure_2,oa_package/47/95/PMC10322670.tar.gz,"['Biopsy of the left lower leg (A) Scanning power demonstrates a dense lymphoid infiltrate involving the reticular dermis and subcutaneous tissue (H E, x 20).']","Figure 2 Biopsy of the left lower leg (A) Scanning power demonstrates a dense lymphoid infiltrate involving the reticular dermis and subcutaneous tissue (H&E, x 20). (B) Higher magnification shows medium to large atypical cells with irregular nuclei (H&E, x 400). Immunohistochemistry shows the neo-plastic cells diffusely positive for CD3 (C; x 400) and CD56 (D; x 400). (E) Ki-67 proliferation index is high (~90%) (Ki-67, x 400)",yes
PMC5181642,Figure_2,oa_package/ed/10/PMC5181642.tar.gz,"['We confirmed that SMN was significantly overexpressed in SMN1SC positive control mice (control + SMN1SC) compared with wild-type mice (control) ( 2000-fold) (E and F).', 'There was no qualitative or quantitative difference in myelin sheath thickness in mice expressing SMN1SC (A and B).', 'There was also no change in the number of non-myelinated large diameter ( 1 m) axons (C) or animal weight (', '.', 'We confirmed that SMN was significantly overexpressed in SMN1SC positive mice ( 2000-fold in control + SMN1SC; 3000-fold in SMA + SMN1SC mice compared with non-SMN1SC carrying control and SMA mice (E and F).']","Figure 2. Overexpression of in Schwann cells is not detrimental. ( ) Representative electron micrograph of a large diameter myelinated axon from the intercostal nerve of a mouse expressing . ( ) Intercostal nerves from control mice expressing SMN had no difference in -ratio measurements compared with control mice (two-tailed, unpaired test; = 3 mice per genotype, > 50 independent nerve fibre measurements per genotype). ( ) No significant increase in numbers of unmyelinated large diameter (>1 m) axons at P7 in control mice expressing ( = 3) ( ) Restoration of SMN to Schwann cells had no detrimental effect on the weight of mice ( > 35 mice per genotype). All tests two-tailed, unpaired tests; = 3 mice per genotype, > 50 independent nerve fibre measurements per genotype. Scale bar = 1 m (A). ( ) Representative immunoblot of intercostal nerve tissue from control, transgenic control mice (control + ), Taiwanese SMA mice (SMA) and SMA rescue mice (SMA + ) demonstrating selective SMN1 overexpression in mice carrying the p - construct. ( ) SMN1 levels were normalized using tubulin levels as a loading control and demonstrated statistically significant SMN1 overexpression in mice carrying the p - construct.",yes
PMC5561130,Figure_2,oa_package/ee/55/PMC5561130.tar.gz,"[' 2, the chorionic membrane and the decidual region of infected mice presented higher expression of all monitored TLRs in comparison with those from non-infected mice.', 'TLRs immunohistochemistry.', 'Placental vascular space impairment is associated with TLR4 activationNext, we sought to determine if iRBCs with P.']","Figure 2 TLRs immunohistochemistry. TLR2, TLR4, and TLR9 immunolabeling in placentas of non-infected and infected mice. Pregnant mice were i.v. infected or not with 110 . NK65 iRBCs on the 13 gestational day. Scores of TLR2, TLR4, and TLR9 expression were defined by using protein expression level of positively stained cells (absent=0, faint=1, moderate=2, intense=3). We considered as low expression TLR intensity scores of 0, 1 or 2, and as high expression scores of 3. 200 magnification, 400 inset. Bar=100m. Dec=decidua; St=Spongiotrophoblast.",yes
PMC10436500,Figure_2,oa_package/49/e3/PMC10436500.tar.gz,"['The main ways that the microbiota can influence the development and function of the nervous system are biological networks, including direct and indirect transmission via chemical transmitters, the immune system, neuronal pathways, and endocrine pathways, as shown in .', 'Communication pathways between the brain and gut microbiota.']","Figure 2 Communication pathways between the brain and gut microbiota. The interaction between the central nervous system (CNS) and gut microorganisms is mediated via several direct and indirect gut-brain axis mechanisms. They include the immune pathway (including cytokines), short-chain fatty acids and microbial metabolites; the neuroactive pathway, including neurotransmitters and neuroactive metabolites; the neural pathway [enteric nervous system, vagus nerve, and spinal nerves ( ); and the endocrine pathway, hypothalamic pituitary adrenal axis (HPA) ( )]. HPA axis response that involves neurons of the hypothalamus that release hormones such as corticotropin receptor hormone (CRH) into the portal circulation or the brain, causing the release of the hormone adrenocorticotropic hormone (ACTH), which starts the production of cortisol and its release. The neuroimmune signaling reactions are regulated by cortisol.",yes
PMC11431435,Figure_8,oa_package/b2/5c/PMC11431435.tar.gz,"['Identification of Cartilage in the Pubic Plate Using Large Microscope Images of Coronal SectionsIn the superior part of the pubis, Safranin O- and Alcian blue-positive cartilage structures were observed at the center (pubic symphysis) in the middle to posterior parts (B,C, red arrows).', 'In addition, a safranin O-positive cartilage structure in the muscle tendon junction that continued from the pubic symphysis was identified in the middle part (B, black arrows).', 'A similar trend was found in the middle and inferior parts (E,F,H,I, red arrows), except for the addition of a cartilage structure in the muscle tendon junction in the inferior anterior part (G, black arrows).', 'Large composite images of coronal sections of the pubis without cleft signs: (A C) Safranin O, Alcian blue, Herovici s, and H E staining of the superior part of the coronal section at the anterior, middle, and posterior levels.']","Figure 8 Large composite images of coronal sections of the pubis without cleft signs: ( ) Safranin O, Alcian blue, Herovicis, and H&E staining of the superior part of the coronal section at the anterior, middle, and posterior levels. ( ) Safranin O, Alcian blue, Herovicis, and H&E staining of the middle part of the coronal sections at the anterior, middle, and posterior levels. ( ) Safranin O, Alcian blue, Herovicis, and H&E staining of the inferior part of the coronal sections at the anterior, middle, and posterior levels. Red arrows indicate cartilage at the pubic symphysis. The black arrows indicate cartilage in the muscletendon junction. Scale bar = 3.2 mm.",yes
PMC7728015,Figure_4,oa_package/62/45/PMC7728015.tar.gz,['RB seeds were seen in 14 specimens stained with fluorescein stain and documented [].'],Figure 4 Showing retinoblastoma vitreous seeds in yellow to orange color with fluorescein stain. Seeds had intra-seed honeycomb appearance unlike drusens (40),yes
PMC6337684,Figure_7,oa_package/cc/ac/PMC6337684.tar.gz,"['The results demonstrated that simultaneous incubation with the PLC inhibitor U73122 (A,E), PKC inhibitor GF109203X (B,F), AC inhibitor MDL12330A (C,G), PKA inactivator H89 (D,H), not only could attenuated the stimulatory action on SL mRNA expression induced by NKB or EGF treatment alone (p 0.', '05), but also notably suppressed the synergistic effect of SL mRNA expression caused by EGF and NKB co-treatment in grass carp pituitary cells (I L).', 'Signal transduction mechanisms of EGF potentiation of NKB-induced SL mRNA expression.']","Figure 7 ( ) Effects of 24-h treatment with the PLC inhibitor U73122 (10 M) or PKC inhibitor GF109203X (10 M) on EGF (10 nM), and NKB (1 M) + EGF (10 nM)-induced SL mRNA expression were examined in grass carp pituitary cells. ( ) Effects of 24-h co-treatment with AC inhibitor MDL12330A (10 M) or PKA inhibitor H89 (10 M) on NKB (1 M) + EGF (10 nM)-induced SL mRNA expression in grass carp pituitary cells. After drug treatment, total RNA was isolated for real-time PCR of SL mRNA expression. In these experiments, the two-way ANOVA was used to test for significant differences among various groups. The asterisk was used to show the difference between single-treating groups and the control group (* < 0.05; ** < 0.01; *** < 0.001; **** < 0.0001). The octothorpe was recruited to present the significant difference between the EGF- or EGF+NKB-treated group and EGF+ or EGF+ NKB+ signal pathway inhibitors combined groups (# < 0.05; ## < 0.01; ### < 0.001; #### < 0.0001).",yes
PMC11460918,Figure_2,oa_package/e4/ff/PMC11460918.tar.gz,['.'],Figure 2. The histopathological images of UCS showing a sarcomatous component (SC) with spindle cells (case 1); (H&E 400).,yes
PMC11327668,Figure_2,oa_package/8f/a0/PMC11327668.tar.gz,['Peroperative image during the first surgery.'],Figure 2 Peroperative image during the first surgery. Visualization of the venous malformation compressing the sciatic nerve.,yes
PMC9468783,Figure_5,oa_package/12/f0/PMC9468783.tar.gz,[],"Figure5 Binding site of core-miRNAs and their effects. The expression of core-miRNAs in paired LC samples in TCGA was shown, and the effects of miRNA/mRNA on the overall survival of LUAD patients were calculated by Kaplan-Meier method. The binding sites of miRNAs were shown. Heatmap shown the expression correlation Coefficient-R of miRNA-mRNA calculated by ENCORI across pan-cancer. The functional enrichment analysis of core-miRNA target genes in LUAD was performed by GSEA through the LinkCompare module of LinkedOmics.",yes
PMC3448180,Figure_10,oa_package/d2/e5/PMC3448180.tar.gz,[],Figure 10 Case 1. 7. Sinus membrane integrity as established by endoscopy.,yes
PMC2409333,Figure_2,oa_package/77/8a/PMC2409333.tar.gz,['Intraoperative images show a large mass within the abdomen and the displacement of the small bowel (left).'],Figure 2 The mass originated from the greater omentum as can be seen on the right.,yes
PMC3519908,Figure_4,oa_package/18/25/PMC3519908.tar.gz,"['Humoral immune response in serum, lungs and nasal swabLung homogenates of Nano-KAg immunized pigs contained significantly higher levels of virus specific IgA and IgG antibodies compared to unvaccinated and K-Ag vaccinated, MN184 challenged pigs (, A B).', 'In the serum samples of Nano-KAg vaccinated pigs increased IgA antibody levels at PC 0 (samples collected on the same day as viral challenge but before inoculating the challenge virus) with a significant increase at PC 15 compared to either unvaccinated or K-Ag vaccinated, MN184 challenged pigs was detected (, D).', 'The PRRSV specific IgG antibody levels in serum (, E) and both IgA and IgG levels in the nasal swab (, G H) were significantly higher in Nano-KAg vaccinated, compared to unvaccinated and K-Ag immunized pigs at DPC 15.', 'In the lungs, a similar trend of increased (but not significant) titers of neutralizing antibodies in Nano-KAg immunized pigs was seen (, C F).', 'g004Enhanced PRRSV specific IgA and neutralizing antibody response in Nano-KAg vaccinated virus challenged pigs.']",10.1371/journal.pone.0051794.g004,yes
PMC5735134,Figure_2,oa_package/bc/65/PMC5735134.tar.gz,"[' 2A D) as well as a corresponding decrease in production of the anti-inflammatory cytokine IL-10 (', ' 2E) as compared to either H.', ' 2F) in H.', 'The resident microbiota acted synergistically with the pathobiont to induce a pro-inflammatory immune response.', '\nThe adaptive immune system is required for severe pathobiont-mediated intestinal inflammationTo assess the role of the adaptive immune response in pathobiont mediated-disease, we switched our mouse model to the C57BL/6 background to compare disease severity for the H.']","Figure 2 The resident microbiota acted synergistically with the pathobiont to induce a pro-inflammatory immune response. ( ) Pro- and anti-inflammatory cytokines present in cecal explants from germ-free (GF), ASF-bearing, mono-associated and colonized ASF bearing mice treated with 1.5% DSS or left untreated ( =912 animals per treatment). ( ) Proportion of effector memory (EM) CD44 CD62L CD4 T cells in mesenteric lymph nodes (each dot represents a pool of 23 animals) of ASF-bearing mice colonized with or without and either treated with DSS or left untreated. ( ) Horizontal bars represent treatment means in all graphs. Asterisks depict the degree of significance for differences as determined by a non-parametric unpaired Mann-Whitney test using a two-tailed distribution for -value calculations (* <0.05, ** 0.01, *** 0.001 and **** 0.0001). Only significant differences between treatments are presented in the graphs. Experiments were performed using male and female C3H/HeN mice at 8-10 wks of age; mice were colonized with for 3 wks.",yes
PMC5031259,Figure_3,oa_package/1d/e4/PMC5031259.tar.gz,"[' 3a).', 'TOP motif mediated enhancement of growth factor synthesis.', '001To explore such regulatory control, we performed motif discovery within the 5 UTR of affected transcripts, because mTOR responsiveness of transcripts can be regulated by presence of a terminal oligopyrimidine (TOP) motif in the 5 UTR [46].', ' 3b), but not in heterozygous cells.', ' 3c).', ' 3d, Additional files 7 and 8: S3A and Table S5).', ' 3e).', ' 3f).', ' 3g and Additional file 9: Table S6).', ' 3d).', ' 3f).']","Fig. 3 TOP motif mediated enhancement of growth factor synthesis. representation of the number of transcripts showing a significant change on the level of translation only ( ; translation changes normalized by transcript level changes) or also on the transcriptional level ( ). Detected de novo motif enrichment using the tool ( < 0.05) within the 5UTR of genes that showed significant TE changes in -deficient cells. for log2 changes of TE comparing heterozygous ( ) and homozygous cells ( ) with control. indicate transcripts with a known 5-TOP motif and indicate all other detected transcripts. of gene set enrichment analysis (GSEA) significance scores comparing transcriptional ( ) and translational ( ) changes in heterozygous ( ) and homozygous cells ( ) versus control. Multiple pathways associated with protein synthesis ( ) are positively regulated specifically on the translational level (FDR < 1E-4). Measurement of translational activity in control, heterozygous, and homozygous mutant cells after six weeks of differentiation. Protein synthesis was measured by flow cytometric detection of incorporation of FITC labeled amino acid analog O-propargyl-puromycin into newly synthesized protein. Histograms display the cell count for different levels of OPP incorporation. Network of vasculature development and tissue remodeling related transcripts that show induction (logFC 2) of translation in the absence of as determined by Fishers exact test (FDR < 0.01). represent transcripts and indicate either gene-biological process associations or protein-protein interactions. Detection of angiogenic growth factors in cultures of -deficient and control cell lines (n=3) using protein arrays. Expression levels in control cells are set to 1 and significance of differential expression is determined by Students two tailed t-test, * <0.05, ** <0.01, *** <0.001",yes
PMC4252312,Figure_5,oa_package/e9/69/PMC4252312.tar.gz,['HLHS after second-stage palliation.'],"Figure 5 HLHS after second-stage palliation. A veno-venous collateral drains to the coronary sinus (stars). In the left pulmonary artery the previously implanted Palmaz Gensis stent can be seen along with the Amplatzer Vascular Plug (black arrow, St. Jude Medical) already deployed in the veno-venous collateral. After release of the second device (white arrow)",yes
PMC5325372,Figure_2,oa_package/4b/10/PMC5325372.tar.gz,['HuR expression in thyroid cell linesPanel A.'],"Figure 2 HuR expression in thyroid cell lines Western blot analysis of HuR expression in a non-tumorigenic thyroid cell lines (Nthy-ori-3.1) and in six tumorigenic ones (SW1736, FTC133, WRO, FRO, BCPAP and TPC1). Densitometric analysis of HuR protein levels in thyroid cell lines. Immunocytochemical staining of Nthy-ori-3.1 and BCPAP cells. The brown signal indicates HuR positivity. Results are shown as mean SD. * p < 0.05, ** p < 0.01, **** p < 0.0001 by ANOVA test.",yes
PMC6826123,Figure_6,oa_package/5d/68/PMC6826123.tar.gz,['Lifelong choline supplementation alters the expression of the Sigma 1 receptor ( 1R) within microglia.'],"Figure 6 Lifelong choline supplementation alters the expression of the Sigma1 receptor (1R) within microglia. (ab) Representative Western blot of 1R levels. Quantitative analysis of 1R protein levels reveals a significant reduction with Ch+ ( <.05; =5/APP group; =4/NonTg group). (cd) Quantitative analysis reveals a significant main effect of genotype, where the APP/PS1 mice have a significantly higher intensity of yellow pixels of 1R/Iba1 colocalization than the NonTg mice ( <.05, =6 APP/PS1 CTL, =5 APP/PS1 Ch+, =6 NonTg CTL, =6 NonTg Ch+). Additionally, we find a significant genotype by diet interaction where the APP/PS1 Ch+ mice show a significant reduction in 1R/Iba1 colocalization than the APP/PS1 CTL mice ( <.001). Photomicrographs depicting the Cornus Ammonis 1 (CA1) of the hippocampus from APP/PS1 and NonTg mice fluorescently stained for the1R and Iba1; images taken at 40; scale bar=25m. Data are presented as box plots. The center line represents the median value, the limits represent the 25th and 75th percentile, and the whiskers represent the minimum and maximum value of the distribution. * <.05, *** <.001",yes
PMC2364763,Figure_2,oa_package/c4/1b/PMC2364763.tar.gz,"['Using MRI, 33 out of 39 (85 ) unfavourable prognosis tumours were correctly identified (example shown in Unfavourable prognosis tumour.']","Figure 2 Unfavourable prognosis tumour. High-resolution T2-weighted fast spin-echo image and corresponding histological (H&E stained) wholemount section. The MRI scan shows widespread discontinuous tumour deposits (arrows) (representing either nodes replaced by tumour or tumour satellites) within the mesorectum, but not extending to the mesorectal fascia (arrow heads). This is confirmed as node-positive disease on corresponding wholemount histology section.",yes
PMC5497981,Figure_4,oa_package/2d/b0/PMC5497981.tar.gz,"['2, respectively), declining towards virus Ct levels around 30 from 8 WPC and onwards (A, Similar to findings in infected rainbow trout, intracellular inclusions containing viral proteins were observed in Atlantic salmon erythrocytes (C), indicating that PRV-Om infects Atlantic salmon and rainbow trout erythrocytes in the same manner as PRV-Ss.', 'However, when using antibodies against dsRNA, positive staining was detected in the cytoplasm of single erythrocytes in lumen of the heart ventricle in one fish (D).']",10.1371/journal.pone.0180293.g004,yes
PMC4115229,Figure_1,oa_package/f9/b0/PMC4115229.tar.gz,"['A histological\nexamination revealed chorionic villi and decidua tissue with fibrinoid necrosis\nfoci and small calcifications in all biopsy specimens\n().', '\nMicrograph of one of the studied placental biopsy\nsamples.']",Fig. 1 Micrograph of one of the studied placental biopsysamples. Hematoxylin- and eosin-stained.,yes
PMC8955340,Figure_1,oa_package/52/9b/PMC8955340.tar.gz,"[' reports the average scores assigned by the two experts after the weighing process, both for each parameter and averaged for all the parameters.', '0328Output from the qualification process.']",Figure 1 Output from the qualification process.,yes
PMC4517882,Figure_4,oa_package/04/da/PMC4517882.tar.gz,"['As previously reported, under growth-promoting conditions, DAF-28::GFP was hardly detected in ASI and ASJ neurons (a and 4e), and it accumulated in coelomocytes of wild-type animals at the adult stage (f and 4h) [g and 4h).', 'The accumulation of DAF-28::GFP was scarcely observed using transgenic animals that expressed gon-1p::gon-1(sig_GON) or daf-28p::gon-1(sig_GON) (c, 4d and 4e).', 'g004Depletion of gon-1 causes DAF-28 secretion defects.']",10.1371/journal.pone.0133966.g004,yes
PMC8145351,Figure_1,oa_package/a2/c2/PMC8145351.tar.gz,"['Another distinctive feature of SARS-Cov-2 pneumonia is large pulmonary vessel involvement with extensive thrombosis into larger vessels ().', '02432425265Summarizes the initial inflammatory activation leading to both coagulative cascade activation and vascular damage, two processes which lead to thrombotic hyperactivity with disseminated intravascular coagulation (DIC) in pulmonary and other districts.']","Figure 1 Summarizes the initial inflammatory activation leading to both coagulative cascade activation and vascular damage, two processes which lead to thrombotic hyperactivity with disseminated intravascular coagulation (DIC) in pulmonary and other districts. Because the infective process is primarily located in the lung, pulmonary circulation is prone to macroscopic thrombus formation and consequent pulmonary embolism (PE).",yes
PMC3377135,Figure_1,oa_package/e6/30/PMC3377135.tar.gz,"['5 mm) [a].', 'The materials and rabbit model with bone defect: (a) materials; (b) model of rabbit femoral condyle bone defect; (c) defects were grafted by CS/PAADegradation studies in vitroPBS (pH=7.']",Figure 1 The materials and rabbit model with bone defect: (a) materials; (b) model of rabbit femoral condyle bone defect; (c) defects were grafted by CS/PAA,yes
PMC9335990,Figure_2,oa_package/d9/d7/PMC9335990.tar.gz,"[' 2A, B), 1 isolated splenic diffused lymphangiomatosis (', ' 2C, D), and 5 cases of splenic focal lymphatic malformation.', ' 2A).', ' 2E, F).', '', 'F D2-40 immunohistochemical staining of Case 20 labels lymphatic endothelium']","Fig.2 Splenic vascular malformations. CT of splenic diffused lymphangiohemangiomatosis (white arrow shows the accessory spleen involved, Case 25). Intraoperative picture of Case 25. MRI of splenic diffused lymphangiomatosis (Case 20). Intraoperative picture of Case 20. HE staining of Case 25 shows lymphatic malformation space filled with eosinophilic amorphous proteinaceous fluid (black arrow) and capillary malformation space filled with blood (hollow arrow). D2-40 immunohistochemical staining of Case 20 labels lymphatic endothelium",yes
PMC10506008,Figure_3,oa_package/10/ef/PMC10506008.tar.gz,['\nContrast-enhanced computed tomography scan of extrahepatic biliary adenoma.'],"Figure 3 A-C: Contrast-enhanced computed tomography scan shows dilatation of the intrahepatic or extra-hepatic bile duct with a slightly enhancement of the mass in left hepatic duct (A), hilar bile duct (B) and distal common bile duct (C). The arrow symbols point to adenomas.",yes
PMC5621192,Figure_7,oa_package/10/2b/PMC5621192.tar.gz,"['74) cases [a], soles in 42 (20.', '(a) Psoriasis over the palms; (b) Trophic ulcer over the solesWe had 6 patients of hand foot mouth diseases with palmoplantar involvement in the form of vesicles with fever, sore throat, and ulcers in the mouth.']",Figure 7 (a) Psoriasis over the palms; (b) Trophic ulcer over the soles,yes
PMC7327932,Figure_1,oa_package/ca/03/PMC7327932.tar.gz,[],"Fig. (1) Schematic illustration of a hypothesized influence of A on GPR39-mediated signaling pathways, which lead to down-regulation of expression of clusterin a protein implicated in Alzheimers disease. In normal, physiological conditions (right) Zn activates GPR39, which leads to up-regulation of clusterin expression Gq PLC - ERK 1/2. Additionally, cross-inhibition of PI3K Akt and cytosolic PKA by ERK 1/2 causes pathway specificity of GPR39 ligand-dependent signaling. In the presence of A (left) ligand-independent activity of GPR39 is promoted. Here, GPR39-bound PKI cannot inhibit cytosolic PKA and activates PI3K Akt, which leads to Raf ERK 1/2 - clusterin inhibition. Red arrow activation, blue T inhibition, AKT protein kinase B, DAG diacylglycerol, ERK 1/2 - extracellular signalregulated kinase 1/2, IP3 - inositol 1,4,5-triphosphate, PI3K - phosphatidylinositol-3-kinase, PIP2 - phosphatidylinositol 4,5-bisphosphate, PIP3 - Phosphatidylinositol (3,4,5)-trisphosphate, PKA protein kinase A, PKC protein kinase C, PKI protein kinase inhibitor , PLC phospholipase C , Raf - proto-oncogene serine/threonine-protein kinase. (Figure composed using Servier Medical Art templates available at: ).",yes
PMC3521634,Figure_2,oa_package/66/70/PMC3521634.tar.gz,"['Cytokeratin 7 (CK7) () and musin 1 (MUC1) () were found to be extensively strongly positive, Cytokeratin 20 (CK20) was negative () in the immunohistochemical staining of the biopsy obtained from rectosigmoid area.', '001""/>Immunohistochemical (IHC) staining of rectal biopsy taken during colonoscopy.']","Figure 2 Immunohistochemical (IHC) staining of rectal biopsy taken during colonoscopy. Positive staining was detected in tumoral glandular structures by immunohistochemical examination of CK7. (CK7 is represented by light brown staining.) (IHC, CK7, 100).",yes
PMC5688437,Figure_1,oa_package/f3/5b/PMC5688437.tar.gz,"[' 1).', 'Lung of cat in Group B before pressure perfusion fixation.', 'No adult HWs were present\nAll the positive control cats (Group C) were found to be infected with adult HWs upon necropsy at the end of study on Day 245 (Table 1).', 'The heart and main pulmonary artery was not enlarged\n6Radiographs of a cat from Group C: Day 110.', '15 caption for details)\n7Radiographs of a cat from Group C: Day 175.', '15 caption for details)\n8Radiographs of a cat from Group C: Day 175.', '15 caption for details)\n9Radiographs of a cat from Group C: Day 240.', '15 caption for details)\n0Radiographs of a cat from Group C: Day 240.', '15 caption for details)\nLung pathologyCats in both Groups B and C had abnormal lungs with turgidity on gross examination (Figs.']",Fig. 1 Lung of cat in Group B before pressure perfusion fixation. The lungs are turgid and especially the right and left caudal lung lobes will not deflate. Discoloration of surface can be visualized in the right caudal lung lobe. No adult HWs were present,yes
PMC3087873,Figure_4,oa_package/24/e4/PMC3087873.tar.gz,"[' 4).', '\nTherapeutic Response can be Successfully Evaluated in the mdx Mouse with CTSB ImagingWe tested the sensitivity of our method to detect therapeutic benefits induced by prednisone, the current standard of treatment for DMD patients, in the mdx model.', ' 4).']","Fig.4 Activated CTSB primarily localizes within regenerating fibers and macrophages. Gastrocnemious muscles taken 5days post-notexin injection revealed co-localization of ProSense, which detects activated CTSB, within newly regenerating fibers (eMyosin), as well as within the cytosol of infiltrating monocytes ( ).",yes
PMC3543763,Figure_4,oa_package/f3/45/PMC3543763.tar.gz,"[' 4).', 'RT-PCR was used to detect changes in the mRNA expression of 5-HT1AR at the different time points after SPS stimulation (A).', 'control group\nMorphological Change of the Oculomotor Nucleus by TEMAs shown in ']","Fig. 4 RT-PCR was used to detect changes in the mRNA expression of 5-HT1AR at the different time points after SPS stimulation ( ). From to , the PCR bands are the following groups: , marker; , control group; , SPS 1d group; , SPS 4d group; , SPS 7d group; and , SPS 14d group. -Actin served as a loading control. Relative amounts of 5-HT1AR mRNA ( ). Data represent the meansSD ( =5, each). denotes <0.05 vs. control group",yes
PMC3485172,Figure_6,oa_package/8d/61/PMC3485172.tar.gz,['Placental gene expression of inflammatory factors.'],"Figure 6 Placentas were collected at G18 (K173 and NK65) or G19 (ANKA ) and RNA expression of Ccl2, Ccl3, Tlr2, Tlr4 and Tnf were evaluated by qReal Time PCR. Relative quantification (RQ) was obtained with normalization by GAPDH. Results are plotted as fold change against non-infected controls collected at the same gestational day (G18 versus K173 or NK65 and G19 versus ANKApm4); Line refers to median values. Kruskal-Wallis Test ** <0.01; *** <0.001 refers to differences between different parasite lines; Unpaired t test # <0.05; ## <0.01; ### <0.001 compares each parasite line to its respective non-infected control.",yes
PMC5106496,Figure_1,oa_package/8f/51/PMC5106496.tar.gz,"['1; unpublished data and data reported in La Morgia and colleagues [113].', '', '90 109, 2015\nShortly thereafter, a study by Alexandrov et al.']","Fig.1 Flat-mount retinas from AD patients exhibit the accumulation of A deposits. Representative microscopic images from a definite AD patient (74years) and a matched control individual (CTRL; 71years) stained with anti-A C-terminal-specific antibody (12F4) and visualized with peroxidase-based labeling (DAB). Blood vessel structures seen as lighter lanes. Classical mature A plaques observed along a retinal blood vessel. , Fluoresence labeling of A -containing depositsdetected in retina of AD patient ( ), using curcumin ( ), 12F4 antibody ( ), and DAPI nuclear staining ( ). Sudan Black B (SBB) is used to quench non-specific autofluorescent signal. Compact extracellular A plaque and cytosolic A accumulations observed following curcumin and anti-A C-terminal-specificantibody (11A5-B10) staining in post-mortem retinas of AD patients. indicate various types of A plaques Images - adopted from La Morgia et al., Annals of Neurology, vol. 79, no. 1, pp. 90109, 2015",yes
PMC11306345,Figure_2,oa_package/3e/69/PMC11306345.tar.gz,"[' 2 summarizes the details.', 'Pathological morphology of the heart, liver, spleen, lungs, and kidneys of each group of mice (HE 400).', 'Content of copper, lead, cadmium, mercury, and arsenic in Indocalamu Iatifolius McClur leavesThen, the content of lead, cadmium, mercury, and arsenic in ""regreened Indocalamu Iatifolius McClur leaves"" and freshly harvested Indocalamu Iatifolius McClur leaves were measured (Table 3).']","Figure 2 Pathological morphology of the heart, liver, spleen, lungs, and kidneys of each group of mice (HE400).",yes
PMC8634449,Figure_1,oa_package/31/fc/PMC8634449.tar.gz,"['The experimental results suggest that we have established a new method of extracellular vesicles-encapsulated EGCG for arthritis treatment ().', '.']",Figure 1. Schematic representation of the assessment of EVs-EGCG regulation of cartilage repair.,yes
PMC9578054,Figure_1,oa_package/d3/49/PMC9578054.tar.gz,"['A non-contrast computed tomography (CT) scan of the brain showed lesions with vasogenic edema in the right parietal lobe ().', '4\nS209\n19\n23717792.']","Figure 1. ( ) Computed tomography of the brain (non-contrast) showed vasogenic edema at the right frontoparietal lobe, right high parietal lobe, and right basal ganglia associated with a 3-mm of midline shift to the left side.",yes
PMC2661536,Figure_1,oa_package/e5/fc/PMC2661536.tar.gz,"['9 cm mass arising from the anterior inferior aspect of the pancreatic head ().', 'CT images from the described case demonstrating metastatic hemangiopericytoma involving the pancreas.']","Figure 1 CT images from the described case demonstrating metastatic hemangiopericytoma involving the pancreas. During the parenchymal phase (A) the mass appears well defined and heterogeneously enhancing. Multiple, prominent feeding vessels surround the mass. Subsequent venous phase imaging (B) further defines the mass which is clearly delineated from the pancreatic parenchyma and has central areas of low attenuation consistent with necrosis. The adjacent duodenum is compressed by resultant mass effect. Curved axial reformatting in the venous phase (C) and curved coronal reformatting in the parenchymal phase (D) better define the position of the mass relative to the pancreatic head. The mass again appears well defined and associated mass effect leads to pancreatic ductal dilatation and compression of the second portion of the duodenum. Numerous dilated, feeding vessels are seen as foci of high attenuation throughout the mass on the parenchymal phase image (D).",yes
PMC7685864,Figure_7,oa_package/e3/51/PMC7685864.tar.gz,"['AA pretreatment in MPTP-treated mice ameliorated MPTP-induced behavioral deficits compared with performances in vehicle-treated MPTP mice (s 7(a) 7(d)).', '006""/>Ascorbic acid pretreatment ameliorates PD-like behavioral deficits and pathophysiology in an MPTP-induced mouse model of PD.']","Figure 7 Ascorbic acid pretreatment ameliorates PD-like behavioral deficits and pathophysiology in an MPTP-induced mouse model of PD. (a) Holding times in the rotarod test were recorded after training mice for three days ( = 6). (b) Grasping strengths were measured using a grip meter after training mice for three days ( = 6). (c) Pole-climbing times were recorded after training mice for three days ( = 6). (d) Motion tracking was monitor in the open-field test ( = 6). (e) The expression of TH in the midbrain was detected by immunohistochemistry (scale bar, 50 m) ( = 3). (f) The expression level of TH in the midbrain was detected by Western blotting ( = 3). Data were obtained from three independent experiments. One-way ANOVAs followed by LSD pairwise comparisons were performed. was considered significant compared to control ( < 0.05, < 0.01, and < 0.001). was considered significant compared to MPTP or MPP ( < 0.05, < 0.01, and < 0.001).",yes
PMC11436413,Figure_2,oa_package/52/be/PMC11436413.tar.gz,"[' 2).', '5 mm) (arrows in c and d)Considering the excellent response achieved with TACE for the two intrahepatic HCC nodules, alongside the persistent contraindications to surgical intervention, multidisciplinary consensus favored attempting TACE treatment for the extrahepatic nodule.']",Fig.2 Contrast-enhanced computed tomography (CT) imaging shows a 5mm peritoneal nodular lesion along the right lateroconal fascia in both the arterial (arrow in ) and portal-venous (arrow in ) phases. The nodule may represent an extrahepatic HCC metastasis possibly secondary to tumor seeding following a prior radiofrequency ablation (RFA). Contrast-enhanced CT performed 3months after the transarterial chemoembolization of the two intrahepatic nodules demonstrates a notable growth of the extrahepatic deposition (10mm vs. 5mm) (arrows in and ),yes
PMC8409052,Figure_2,oa_package/19/2d/PMC8409052.tar.gz,"['Optical coherence tomography (OCT) demonstrated corresponding parafoveal foci of outer nuclear layer hyperreflectivity with granularity of the underlying ellipsoid ().', ')3DiscussionAMN is an uncommon retinal vascular disorder that predominantly affects young, non-Latino white women.']","Fig. 2 . Near-infrared scanning laser ophthalmoscope (SLO) fundus image and Optical Coherence Tomography (OCT) of the right and left eye on post-vaccination day 6, 13, and 31. There are triangular dark lesions (yellow chevron) in the superior macula with corresponding bilateral focal ellipsoid zone loss (blue chevron) worse in the right eye. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)",yes
PMC5606113,Figure_5,oa_package/72/73/PMC5606113.tar.gz,"['5,\na-c).', '5d, time-dependent changes of these two markers exhibit similar patterns.', '5, a and b).', '5e) showed the same trend of the Western blot data.', 'Changes of LC3-II and CHOP protein levels during PR degeneration in rd10 retinas with or without S1R.', 'Immunostaining of LC3 on retinal sections collected at indicated time points\nBiphasic effect of S1R knockout in rd10 mice on rod PR electrophysiologyThe foregoing opposite outcomes at early and late time points prompted us to determine a 3 W 6 W full-spectrum impact of S1R knockout on rods, considering that rod degeneration occurs earlier and consequently leads to cone death [25].']","Fig. 5 Changes of LC3-II and CHOP protein levels during PR degeneration in rd10 retinas with or without S1R. and . Western blots detecting LC3-I (uncleaved), LC3-II (cleaved and lipidated, marker of autophagosomes), and CHOP in retinal homogenates collected at indicated time points. The postnatal day-19 experiment (see ) was performed about 6months after that in , triplicate samples (each from two retinas in one mouse) were used for the same blot. and . Quantification of Western blots shown in and , respectively. For the method of quantification refer to Fig. ; meanSE; =4 mice; * <0.05, ** <0.01, *** <0.001. . Immunostaining of LC3 on retinal sections collected at indicated time points",yes
PMC7064898,Figure_4,oa_package/39/3e/PMC7064898.tar.gz,['\nSOM positive interneurons are highly affected in the perirhinal cortex of AD patients.'],"Figure 4 Double immunolabeling for SOM (dark brown) and the neuronal marker NeuN (orange) in the perirhinal cortex; a1) panoramic view of the perirhinal cortex showing SOMpositive neurons distribution in layers IVI in Braak II (agematched controls); a2a4) higher magnification of the deep cortical layers (IVVI) in Braak II (a2), Braak IIIIV (a3) and Braak VVI (AD patients, a4). A decrease in the number of SOMimmunostained somata (blue arrows) is observed in Braak IIIIV and Braak VVI samples along with a reduction of the SOMpositive axonal plexus. Cell morphology details of SOM neurons in the perirhinal cortex of Braak II (a5) and Braak VVI (a6 anda7) cases are shown in higher magnification images. SOMimmunoreactive dystrophic neurites (purple arrows) are detected in Braak VVI samples associated with plaques, as demonstrated by the confocal image (a8, SOMdystrophies are pointed out with white arrows). The numerical density (cells/mm ) of SOMpositive neurons in the perirhinal cortex of Braak II (n=5), Braak IIIIV (n=3) and Braak VVI (n=5) cases was determined by stereology. Quantitative data confirm the existence of an extensive significant loss of SOM cells in Braak IIIIV and Braak VVI patients. Data (meanSD) were analyzed by oneway ANOVA <0.001 ( (2, 10)=14.428 followed by Tukey post hoc multiple comparison test. Significance * <0.05, ** <0.01) is indicated in the figure. . Scale bars: a1, 200m; a2a4, 100m; a5a7, 25m; a8, 10m.",yes
PMC11235567,Figure_2,oa_package/7d/02/PMC11235567.tar.gz,"['Intraoperative findings show a distinct, well-demarcated round mass (black arrow) after dissecting the descending colon (white arrow).']","Figure 2 Intraoperative findings show a distinct, well-demarcated round mass (black arrow) after dissecting the descending colon (white arrow).",yes
PMC4152129,Figure_4,oa_package/23/ad/PMC4152129.tar.gz,"['0104416-Akiyama1"" ref-type=""bibr"">[26]) were more abundant in the optic nerve pre-laminar region of LPS-treated compared to control mice (\na f\n) and their number correlated with RGC loss (\ng\n) suggesting that peripheral LPS administration leads to local activation of microglia in the ONH.', 'g004Peripheral LPS administration leads to microglial cell changes in the ONH and retina.', 'To detect subtle changes in microglial activation, we performed Sholl (\nh,i\n), skeleton (\nj\n) and morphological ( S5 in Additional file 1: Supplementary .']","Fig. 1 Steps of semi-automatic white matter (WM) lesion segmentation in one of our patients (central coronal slice): Lesions in the original fluid-attenuated inversion recovery (FLAIR) image appear as hyperintensities. After threshold adjustment (green voxels), WM lesions could be segmented (light red voxels)",yes
PMC9302378,Figure_8,oa_package/32/ce/PMC9302378.tar.gz,[],"FIGURE 8 Effect of systemic LPS treatment on prion disease-specific PrP accumulation in the brain. Mice were first injected with ME7 scrapie prions directly into the brain by IC injection. Thirty five days later the mice were given a either single IP LPS injection (prions + 1xLPS) or four consecutive daily IP LPS injections (prions + 4xLPS) to induce innate immune training or tolerance, respectively. A parallel group of mice were given four consecutive daily IP PBS injections (prions + 4xLPS) as a control. Brains were collected at the terminal stage. Western blot analysis of prion disease specific PrP accumulation in half brains from each group. PK, proteinase-K. Upper panel, total PrP; middle panel, relatively PK-resistant prion disease-specific PrP ; lower panel -actin. Quantitation of the relative abundance of PrP in half brains from each group. The abundance of PrP in each sample was expressed as the% relative to the mean value in the 1xPBS-treated controls. = 56 mice/group; horizontal bar in histograms, median.",yes
PMC5507798,Figure_5,oa_package/32/21/PMC5507798.tar.gz,"['Cultures for AFB from cervical lymph nodes were reported positive for Mycobacterium tuberculosis complex identified by direct molecular detection real-time PCR; pathology stain demonstrated AFB ().', '.']",Figure 5. Cervical lymph node acid fast stain showing acid fast bacillus in the multinucleated giant cell (black arrow).,yes
PMC8719289,Figure_2,oa_package/ca/ba/PMC8719289.tar.gz,[],Fig. (2) Axial T2-weighted fluid-attenuated inversion recovery image in a patient 3 weeks after trauma shows hyperintense anterior frontal lobe parenchymal lesions that are consistent with hemorrhagic contusions (arrows). These lesions also demonstrate hyperintense signal on T1-weighted images (arrows) and a rim of susceptibility artifact on susceptibility-weighted imaging (arrows). ( ).,yes
PMC8041575,Figure_3,oa_package/52/42/PMC8041575.tar.gz,['.'],Fig. 3. Brain computed tomography scan images of a patient who received the Sengstaken-Blakemore tubes. (A) Inflated esophageal balloons (*) in the nasal cavity. (B) inflated gastric balloon (+) in the oral cavity. Informed consent for publication of the clinical image was obtained from the patient.,yes
PMC8630953,Figure_6,oa_package/11/3e/PMC8630953.tar.gz,"['On MRI,\nspinal cord schistosomiasis typically presents as conus medullaris expansion,\nwith a signal that is hypointense on T1-weighted images and hyperintense on\nT2-weighted images, with contrast media uptake (), allowing the differential diagnosis to be made with\nanti-MOG antibody-associated demyelinating disease.', 'Spinal cord schistosomiasis.']","Figure 6 Spinal cord schistosomiasis. Sagittal T2-weighted MRI sequence (A)showing a central area of increased hyperintensity with poorly definedborders, together with an increase in the volume of the conus medullaris(arrow), resulting in obliteration of the corresponding anterior andposterior cerebrospinal space. Contrast-enhanced T1-weighted sequence(B) showing a heterogeneous tree-in-bud enhancement pattern (arrow).Courtesy of Dr. Gustavo Balthazar.",yes
PMC5613452,Figure_1,oa_package/f9/94/PMC5613452.tar.gz,"[' 1).', 'Calcaneal region of interest (ROI) constructed on various cross sections\nAn open-source platform, OsiriX MD (v.', ' 1).']",Fig. 1 Calcaneal region of interest (ROI) constructed on various cross sections,yes
PMC9503863,Figure_4,oa_package/a0/73/PMC9503863.tar.gz,"['Six out of nine animals inoculated with ASFV-Kenya-IX-1033- CD2v had mild to severe fibrinous pericarditis at post-mortem investigation, whereas no pericarditis was observed in the PBS group ().', 'Gross-pathology after immunisation with ASFV-Kenya-IX-1033- CD2 and challenge with ASFV-Kenya-IX-1033.']",Figure 4 Gross-pathology after immunisation with ASFV-Kenya-IX-1033-CD2 and challenge with ASFV-Kenya-IX-1033. ( ) Representative pictures of hearts of pigs after mock immunisation with PBS and challenge with ASFV-Kenya-IX-1033. ( ) Representative pictures of hearts of pigs after immunisation with ASFV-Kenya-IX-1033-CD2 and challenge with ASFV-Kenya-IX-1033.,yes
PMC5642267,Figure_2,oa_package/72/55/PMC5642267.tar.gz,"['In several adenomas with a mixed cell composition of oxyphilic and chief cells, we observed that the inflammation was predominantly located to the oxyphilic areas ().', 'Photomicrographs of routine histology (Htx-eosin, left) and immunohistochemistry for CD45 (right) of a parathyroid adenoma with mixed cell type.']","Figure 2 Photomicrographs of routine histology (Htx-eosin, left) and immunohistochemistry for CD45 (right) of a parathyroid adenoma with mixed cell type. Inserts show histologically evident presence of diffusely infiltrating lymphocytes in areas with oxyphilic-(black arrow) but not chief cell differentiation. Immunohistochemical staining for CD45 also revealed presence of lymphocytes in the chief cell areas. There were also prominent perivascular infiltrates of lymphocytes, with one small aggregation of CD20 and CD8+ cells (red arrow, CD8 and CD20 staining not shown).",yes
PMC10577053,Figure_1,oa_package/c5/4d/PMC10577053.tar.gz,"['0 cm intraabdominal abscess (see ', '']","Fig.1 Axial CT angiogram of the abdomen in the arterial phase demonstrating large rim-enhancing fluid collection (blue arrow) in the right upper quadrant consistent with abscess. This collection abuts both the colon (yellow arrow) and duodenum (white arrow). There is no visualized site of bowel perforation, although this is not excluded.",yes
PMC11430971,Figure_1,oa_package/ca/f5/PMC11430971.tar.gz,"['1 compared with HOME1 or HOME50 for both cell lines (A C).', 'Likewise, EPC1 and EPC2 formed organoids poorly in ADF-based medium referred to as advanced DMEM+/+/+ (herein ADF3+) containing 50 ng/mL EGF [40] (A C, Table 3) that was utilized to generate 3D organoids from human oral mucosa consisting of non-cornifying stratified squamous epithelium.', '1 was comparable with that in KSFMC (A C, Table 3).', 'HOME0 Permits Normal Esophageal Organoid Formation from Patient BiopsiesGiven improved OFR and the differentiation gradient formation for EPC1 and EPC2 cells in HOME0 ( and ), we further tested HOME0 on PDO formation with normal esophageal endoscopic biopsies.', 'Given the oncogenic role of EGFR in esophageal carcinogenesis, normal human esophageal cells may negate malignant transformation via degenerative changes in response to excessive mitogenic stimuli as observed in HOME50 ().', '1038/s41467-023-41039-637669937\nLow EGF concentrations ( 0.']","Figure 1 Low EGF concentrations (0.1 ng/mL) may facilitate organoid formation in ADF-base medium. EPC1 and EPC2 cells were seeded at a density of 5000 cells per 50 L Matrigel and fed with KSFMC, HOME, or ADF3+, containing human recombinant EGF at indicated final concentrations and allowed to form organoids. ( ) Resulting structures were captured by Keyence and photomicrographed. Scale bar, 200 m. ( , ) OFR was determined and plotted in graphs. *, < 0.05 vs. 0 ng/mL EGF in HOME (i.e., HOME0); NS, not significant vs. HOME0, n = 3 per group. One-way ANOVA was used for the overall comparison.",yes
PMC8291943,Figure_2,oa_package/b1/14/PMC8291943.tar.gz,"['7 cm revealed the pathological findings shown in figure 2.', 'Nodular lymphoid hyperplasiaHE staining of a nodular lesion showed advanced inflammatory cell infiltration (figure 2a and b).', 'Immunostaining showed that the lesions consisted of mixed CD3- and CD20-positive T- and B-cells (figure 2b).', 'The ratio of kappa- and lambda-positive cells in CD79 -positive cells was not significant, suggesting no monoclonality of B-cells (figure 2c).', 'Although CD79 -positive cells were also positive for IgG, almost no IgG4-positive cells were present (figure 2d).']","Figure2 Macroscopic, pathological and immunohistochemical analyses of lung nodules. a) Macroscopic appearance of the resection of segment 9 of the right lower lobe. b) Haematoxylin and eosin (HE) stain, CD20 and CD3 immunohistochemistry (IHC) staining (Magnification of 40x). c) CD79a, kappa and lambda light-chain IHC staining (Magnification of 40x). d) CD79a, IgG and IgG4 IHC staining (Magnification of 40x). Scale bars=200 m.",yes
PMC7475504,Figure_3,oa_package/56/63/PMC7475504.tar.gz,[],"Figure S1 Immunohistochemical staining for ER, PR, HER2 Ki-67 and fluorescence hybridization (FISH) for HER2 (100).",yes
PMC8028859,Figure_4,oa_package/6c/0d/PMC8028859.tar.gz,['Morphology and immunohistochemistry of neuroglial cells in totally demyelinated multiple sclerosis optic nerve (MSON) with demyelination lesion type A (DMA).'],"Figure 4 Morphology and immunohistochemistry of neuroglial cells in totally demyelinated multiple sclerosis optic nerve ( with demyelination lesion type ( ). A, B, DH. Luxol fast blue ( )stained sections at overview (A) to high power magnification show the presence of cells with small round nuclei (arrows in D, F) that are closely associated with thin ( , C) myelin sheaths in some fascicles (outlined in red on overview, A and boxed in red; BD, H) but not in others (outlined in green on overview, A and boxed in green; EG). IL. Immunohistochemistry with labeled almost all of these cells, resulting in extensive staining throughout the demyelinated fascicles (I). JL. At high magnification, staining was seen in two distinct patterns. J, K. In the majority premy ls (individual images boxed in green), radiating processes were seen extending out from perinuclear cell bodies to sometimes end in periaxonal cuffs (arrows), whereas remy ls (individual images boxed in red) lacked visible processes such that their perinuclear positivity was separated from nearby periaxonal rings (arrows in L). Further analysis showed a similar discriminative pattern with the markers (M, N) and (O, P). C. Immunohistochemistry with was restricted to the thin myelin sheaths of remy ls. QR. In addition to premyOls and remyOls, antigenic phenotyping identified occasional OPC and OP. R. Following the SIMPLE (Sequential Immunoperoxidase Labeling and Erasing) protocol, an OPC nucleus at the top of the outlined example is faintly stained with haematoxylin remaining from the previous imaging stage and encircled by Vim+ cytoplasm that extends distally to wrap around nearby axons, (Vim preceding HNK and final counterstain). Q. 2 cells/ were identifiable by nuclear lig1 expression (arrowhead) in contrast to premy ls or remy ls that had weak cytoplasmic staining (arrow). S. Dual staining identified multiple neuroglial cell types simultaneously within a single preparation. lig2 combined with (brown) and contrasted with Vim (red), separated premy ls or remy ls with small round (weakly) lig2 nuclei and cytoplasm ( lig2 / /Vim ; arrows) from 2 cells/ that had intense lig2 nuclei but no cytoplasmic staining (Olig2 / /Vim ; arrowhead), astrocytes with large nuclei and coarse straight processes ( lig2 / /Vim ; open arrows) and immunonegative microglia ( lig2 / /Vim ; circled). Scale bars=1mm (A); 50m (E, I); 20m (F); 10m (PR); 5m (G, H, JS).",yes
PMC3891989,Figure_3,oa_package/e3/19/PMC3891989.tar.gz,['Laryngoscopic findings.'],"Figure 3 The pharyngeal space was narrowed, and the larynx was severely compressed by the hematoma.",yes
PMC10629042,Figure_3,oa_package/5f/f8/PMC10629042.tar.gz,"[' 3).', 'B Axial section of T2 weighted magnetic resonance imaging gradient echo displaying anterior cervical epidural hematoma at the level of C6 (right, red arrow)Therapeutic intervention/outcomesThe neurosurgical service was consulted, and the patient was evaluated by their team.']","Fig. 3 Axial section of T2 weightedmagnetic resonance imaging gradient echo displaying anterior cervical epidural hematoma at the level of C3 (left, red arrow). Axial section of T2weighted magnetic resonance imaging gradient echo displaying anterior cervical epidural hematoma at the level of C6 (right, red arrow)",yes
PMC9399815,Figure_1,oa_package/0c/d4/PMC9399815.tar.gz,"['A loss of neutralization capacity can ensue, as well as facilitation of infection of susceptible immune cells (A; Halstead and O Rourke, 1977; Halstead, 1979; Dejnirattisai et al.', ', 1999, 2002b,1999), can lead to overabundant Fc-mediated recruitment of immune cells, inflammation, Th2-biased responses and increase antibody production (Anderson and Mosser, 2002), subsequently enhancing the disease (B).', ', 2015) following vaccination with the FI-RSV and FI-MV vaccines (C).']","FIGURE 1 Proposed mechanisms leading to vaccine-associated enhancement of disease. Antibody-dependent enhancement (ADE), in which cross-reactive vaccine-elicited antibodies fail to neutralize the dengue virus and instead promote viral replication in FcR-bearing cells. Immune complex accumulation and deposition in the lungs, promote (1) an inflammatory environment, (2) the production of antibodies, and (3) activation of the complement cascade. Skewing of the immune response toward a Th2 phenotype, followed by eosinophil infiltration and accumulation in the lungs. .",yes
PMC4444170,Figure_4,oa_package/80/98/PMC4444170.tar.gz,"['Static UT, ).', 'The CM from sheared osteoblasts (FFS UT) induced up-regulation of collagen X (Col10a1), alkaline phosphatase (Alpl), and Mmp13 mRNAs in chondrocytes relative to CM obtained from static control cells ().', 'chondrocytes treated with CM from FSS UT ().', 'g004The early OA-like phenotype of chondrocytes is attenuated when treated with conditioned media derived from osteoblast sheared in the presence of the T-VSCC inhibitor, NNC55-0396 (NNC).']",10.1371/journal.pone.0127290.g004,yes
PMC7766006,Figure_2,oa_package/f2/00/PMC7766006.tar.gz,"['In the presence of IGF-I (10 nM), hepatocytes accumulated significantly more fluorescence, suggesting a stimulatory action of IGF-I on A uptake by these cells (A).', 'Moreover, liver IGF-I deficient (LID) mice with a 70% reduction in circulating IGF-I [30] showed reduced liver accumulation of tagged A after intravenous injection, while blood levels were increased, as compared to controls, indicating reduced liver clearance (B).', 'Of note, IGF-I treatment of LID mice ameliorated these deficits (B).', 'However, as in overweight mice, LID mice did not show changes in brain A 1-40 levels (C).', 'Modulation by IGF-I of A uptake by hepatocytes.']","Figure 2 Modulation by IGF-I of A uptake by hepatocytes. ( ) IGF-I induces uptake of A by hepatocytes ( = 6). Representative micrograph of cultured hepatocytes with internalized fluorescent A (green). Cell nuclei stained with Hoescht. Lower histograms: Quantification of intracellular fluorescent A after IGF-I treatment. ( ) Serum IGF-I deficient mice (liver IGF-I deficient (LID) mice) show reduced A uptake by the liver, which was ameliorated by systemic IGF-I treatment ( = 5 control/6 LID/5 LID + IGF-I). ( ) Brain A levels did not change in LID mice ( = 8). * < 0.05 and *** < 0.001.",yes
PMC4113646,Figure_2,oa_package/7b/46/PMC4113646.tar.gz,"['Fibrosis and inflammatory cell infiltration were not observed, with apoptosis present only in small areas (A).', 'In groups 2 and 3, the tumor tissues of the nude mice exhibited a small area of tumor necrosis (B and C); however, necrosis was observed in the majority of the regions in group 4.', 'Tumor cell structures were unclear and inflammatory cells were observed in the necrotic tissues (D).', 'Pathological sections of nude mouse liver metastatic tumor tissues in groups (A) 1, (B) 2, (C) 3 and (D) 4 (hematoxylin and eosin stain; magnification, 200).']","Figure 2 Pathological sections of nude mouse liver metastatic tumor tissues in groups (A) 1, (B) 2, (C) 3 and (D) 4 (hematoxylin and eosin stain; magnification, 200).",yes
PMC7758145,Figure_7,oa_package/39/00/PMC7758145.tar.gz,"['The tumor growth rates in mice injected with siRNAs-COL17A1 cells were slower than in the control mice (s 7(a) 7(d)).', '006""/>Downregulated COL17A1 reduced the tumor growth in vivo.']","Figure 7 Downregulated COL17A1 reduced the tumor growth in vivo. (ac) The volume of the tumor xenograft of nude mice in the si-COL17A1 group was smaller than that in the control group. (d) Tumor xenograft growth of nude mice in the si-COL17A1 group was slower when compared to the control group ( < 0.05, < 0.01, and < 0.001).",yes
PMC10873109,Figure_3,oa_package/8b/21/PMC10873109.tar.gz,['.'],"Figure 3. APOE4 promotes acetylation of HMGB1 and decreases levels of SIRT1 deacetylase, and removal of neuronal APOE4 reduces HMGB1 acetylation and increases SIRT1 levels (A) Representative images of anti-acetyl-HMGB1 (green) and anti-GAPDH (red) western blots in hippocampal tissue lysates. (B) Quantification of acetyl-HMGB1 levels relative to GAPDH. (C and D) Quantification of acetyl-HMGB1 levels relative to GAPDH in the nuclear fraction (C) and cytoplasmic fraction (D). (E) Representative images of anti-SIRT1 (green) and anti-TUJ1 (red) western blots in hippocampal tissue lysates. (F) Quantification of SIRT1 levels relative to TUJ1. (G) Representative images of neurons stained with anti-SIRT1 in the hippocampus (scale bar, 60 m). (H) Quantification of the integrated density of SIRT1 in hippocampal neurons. (I) Representative images of immunostaining with anti-HMGB1 and DAPI in hippocampal neurons (scale bar, 40 m). (J) Representative high-magnification images of immunostaining with anti-HMGB1 and DAPI in hippocampal neurons (scale bar, 10 m). (K and L) Quantification of the nuclear integrated density (K) and extranuclear integrated density (L) of HMGB1 immunostaining in hippocampal neurons. (M and N) HMGB1 protein levels measured by ELISA in all hippocampal interstitial fluid (ISF) fractions (M) and in each collected ISF fraction (N). (O) Representative images of anti-acetyl-HMGB1 (green) and anti-TUJ1 (red) western blots in hippocampal tissue lysates. (P) Quantification of acetyl-HMGB1 levels relative to TUJ1. (Q) Representative images of anti-SIRT1 (green) and anti-TUJ1 (red) western blots in hippocampal tissue lysates. (R) Quantification of SIRT1 levels relative to TUJ1. (S) Representative images of neurons stained with anti-SIRT1 in hippocampus (scale bar, 60 m). (T) Quantification of the integrated density of SIRT1 in hippocampal neurons. Data in (B), (C), (D), (F), (P), and (R) are quantified by western blot analysis of hippocampal tissue lysates. For all representative images and quantified data, mice were 10 months of age and belonged to PS19-E4, PS19-E3, or PS19-E4/Syn1-Cre group as indicated. Quantified data in (B), (C), (D), and (F) (PS19-E4, n = 7; PS19-E3, n = 7); in (H) (PS19-E4, n = 12; PS19-E3, n = 14); and in (K), (L), (P), (R), and (T) (PS19-E4, n = 6; PS19-E4/Syn1-Cre, n = 6) are represented as the mean SEM, unpaired two-tailed t test. Fractions 1 and 2 were excluded from analyses in (O) and (P). Quantified data in (M) and (N) (PS19-E4, n = 4; PS19-E4/Syn1-Cre, n = 3) are represented as the mean SEM, unpaired two-tailed t test.",yes
PMC5447637,Figure_4,oa_package/64/62/PMC5447637.tar.gz,"['When the active tip of an electrode ablates the feeding artery, intranodular linear echogenicities are generated, which then spread to the periphery of the target nodule ().', 'RFA = radiofrequency ablationArtery-first ablation technique.']","Fig. 4 Artery-first ablation technique. Intranodular linear echogenicities (arrows) spreading to periphery of target nodule can be seen, which may be microbubbles filling arterioles.",yes
PMC9003763,Figure_5,oa_package/83/61/PMC9003763.tar.gz,"['1177_15385744211068648-fig5"" position=""float""/>.']","Figure 5. Single stent, isolated common femoral artery. Initial angiogram demonstrated moderate stenosis in the common femoral artery with severe proximal stenosis in the proximal superficial femoral artery (A). The common femoral artery was pre-dilated with an 8-mm angioplasty balloon with subsequent deployment of a 7 x 40mm SUPERA stent in the common femoral artery (B). Post-angioplasty angiogram revealed adequate angiographic results (C).",yes
PMC3519908,Figure_2,oa_package/18/25/PMC3519908.tar.gz,"['Potential of PRRSV Ags entrapped nanoparticle (Nano-KAg) as a candidate vaccineIn a pre-challenge study, intranasal delivery of Nano-KAg resulted in induction of innate immune response at both mucosal and systemic sites, indicated by a significant increase in the frequency of NK cells, DCs, and T cells in the lung MNC (, A C); and T cells and DCs in the PBMC compared to K-Ag vaccinated pigs (, H I).', 'Immune cells involved in adaptive arm of the immune response, such as CD4+ CD8+ T cells (Th/memory) and CD8+ T cells were increased significantly in the lung MNC of Nano-KAg compared to K-Ag vaccinated pigs (, D E).', 'Further, lung MNC and PBMC from Nano-KAg immunized pigs secreted significantly reduced levels of the cytokine, IL-10, and higher amounts of IL-6 in a recall response (, F, G, J).', 'In addition, innate cytokine IFN- was secreted at significantly higher levels in pigs vaccinated with Nano-KAg compared to both the control groups (, K).', 'g002Nano-KAg elicited enhanced innate and suppressed regulatory response in a pre-challenge study.']",10.1371/journal.pone.0051794.g002,yes
PMC7790158,Figure_4,oa_package/80/1c/PMC7790158.tar.gz,"['18,19Registered field of view in CT data of patients A and B (Appendix source data 2.', 'Input for F and G.', ' source data 3.', ' figure supplement 1.', 'The marked difference in sphingomyelin partitioning between the two granule types was validated in both purified granule fractions and intact cells using a secretory pathway fluorescent biosensor, EQ-SM-Kate (Deng et al.', 'mKate and GFP fluorescence of purified granules from INS 832/13 cells that had been co-transfected with both biosensors was measured before and after immunodepletion with antibodies against either syt7 or syt9 (B).', 'In contrast, the SM-Kate signal only decreased when syt7 granules were depleted (B and C).', 'Punctate fluorescence signals were observed for both constructs (D).', 'The EQ-SM-Kate signal predominantly colocalized with the GFP signal while there were numerous GFP-positive puncta that lacked the EQ-SM-Kate signal (D and E).', 'The relative abundance ratios of all detected lipid species in syt9 over syt7 granules were determined along with their significance values ( source data 2) and plotted in a log-log volcano plot (F).', '05 (F).', ""When sphingomyelin 42:1, which likely contains the two predominant sphingomyelin tails, 18:0 in the sphingosine and 24:1 in the acyl chain positions (O'Brien and Rouser, 1964), is visualized in a bar graph and compared to all other pooled lipid species in each headgroup class, it is apparent that sphingomyelin 42:1 is 3-fold enriched in syt7 compared to syt9 granules (G)."", 'The striking difference in lipid composition between the two granule subpopulations, especially with regard to sphingomyelin and cholesterol content (), almost certainly contributes to the specific sorting of the fusion regulatory proteins and transporters ().', 'The amount of cholesterol or sphingomyelin per amount of protein in the sample was determined for control and immunodepleted samples ( figure supplement 1).', 'The relative amounts of cholesterol and sphingomyelin as compared to the control sample are shown (A) by normalizing the immunodepleted samples to equal amounts of samples that were not immunodepleted.', 'The normalized data was then log2 transformed and the data represented in a bar-graph (G).', '05) of individual lipid species were included and compared to average class changes in G.']","Figure 1figure supplement 3. Averaged FCS correlation curves along with curve fits. ( of insulin granules purified from palmitate-treated (FFA), cytokine-treated, and Doc2B-expressing cytokine-treated GRINCH cells or immunodepleted with antibodies against syt7 or syt9, and controls without treatments or immunodepletion are averaged from 20, 30 s runs. The hydrodynamic radius for each curve takes into account variations in temperature and viscosity with errors reported between fits. Fractional triplet component is reported where necessary.",yes
PMC1524979,Figure_3,oa_package/a9/30/PMC1524979.tar.gz,"['Atrophy-like pattern (ASC-H, favor atrophy).']","Figure 3 (ASC-H, favor atrophy). . Single cell pattern. Isolated cells with hyperchromatic atypical nuclei with smudgy chromatin). (a & b- Cervical smear [Papanicolaou stained SurePath Preparation]). . HCG of parabasal cells. Cohesive hyperchromatic crowded groups of small parabasal cells with high N/C ratio. The nuclei are relatively small and show nucleoli (arrow). (a & b- Cervical smear [Papanicolaou stained SurePath Preparation], c- Cervical biopsy [Hematoxylin-eosin stained section]).",yes
PMC8715830,Figure_4,oa_package/a7/f5/PMC8715830.tar.gz,[],"FIGURE 4 HSC70 accelerates myosin binding protein C3 (MYBPC3) protein degradation via lysosomal pathway in Ang IItreated induced pluripotent stem cellderived cardiomyocyte (iPSCCM) overexpressing MYBPC3 truncation mutations. (A) Western blot analysis of MYBPC3 expression in WT and mutant iPSCCMs after YM1 treatment. GAPDH is used for the loading control. (B) Bar graph to compare the MYBPC3 expression between different groups in A. =34 culture replicates. <0.05. (C) Western blot analysis of MYBPC3 expression in WT and mutant iPSCCMs after VER155008 treatment. GAPDH is used for the loading control. (D) Bar graph to compare the MYBPC3 expression among different groups in C. =35 culture replicates. <.05. (E) Bar graph to compare the MYBPC3 expression between different groups in Figure . =35 culture replicates. <.001 and <.0001. (F) Bar graph to compare the MYBPC3 expression between different groups in Figure . =45 culture replicates. <.01, <.001 and <.0001. (G) Proposed work model. Ang II stress in iPSCCMs overexpressing MYBPC3 truncation mutations (L460fs and G853fs) causes a variety of deleterious phenotypes, including reduced MYBPC3 expression, hypertrophy, arrhythmia and elevated diastolic [Ca ] . HSC70 plays a pivotal role in the turnover of MYBPC3 via lysosomal pathway, which can be inhibited by chloroquine (CQ). The manipulation of HSC70 activity by YM1 (HSC70 activator) or VER155008 (HSC70 inhibitor) can regulate the content of MYBPC3 protein. In Ang IItreated mutant iPSCCMs, the upregulated HSC70 accelerates the MYBPC3 degradation and results in the deficiency of MYBPC3 protein, which can be partially rescued by VER155008. The reduced MYBPC3binding ryanodine receptor 2 (RYR2) caused by insufficiency of MYBPC3 protein may give rise to excessive free destabilized RYR2, which in turn causes larger RYR2mediated Ca leak. The resultant elevated Ca loading may trigger the development of both hypertrophy and arrhythmogenesis, particularly under stress conditions",yes
PMC7743069,Figure_2,oa_package/a3/e1/PMC7743069.tar.gz,"['At 24 hpi with Spp lentivirus, many pathological changes in the lungs were evident, including multifocal lesions, inflammatory cell infiltrations, thickened alveolar walls, peri-vascular and peri-bronchial infiltrations and fibroplasia with exudation of fibrin and proteins ().', 'We observed only mild inflammations such as mildly thickened alveolar walls in the lungs in the VSV-G group at 24 hpi ().', '414706-f0001"" position=""float""/>Mice acquires acute pneumonia 24 hours post-infection (hpi) of Spike protein-pseudotyped (Spp) lentivirus.']","Fig 2 Mice acquires acute pneumonia 24 hours post-infection (hpi) of Spike protein-pseudotyped (Spp) lentivirus. Histological analysis of lungs in control mice (A), mice being administrated with lentivirus carrying vesicular stomatitis virus glycoprotein (VSV-G) (B), or Spp lentivirus (C and D) shows acute and diffuse inflammatory responses in the lungs after 24 hpi of Spp. Right panel is the magnification of boxed area in the corresponding left panel. Scale bar: 500 m (left panel) and 100 m (right panel).",yes
PMC10247946,Figure_5,oa_package/a7/46/PMC10247946.tar.gz,"[' 5), or Amplatzer plugs.', 'Splenic artery aneurysm managed by front and back door embolisation using vascular plug for the front door and coils for the back door.', 'D 2 months follow up arterial phase post contrast CT confirming exclusion of the aneurysm from the circulation with less than 20% infarction of the splenic parenchyma without clinical significanceHepatic Artery Aneurysms (HAAs)HAAs are the second most common true VAA.']",Fig. 5 Splenic artery aneurysm managed by front and back door embolisation using vascular plug for the front door and coils for the back door. Axial arterial phase post contrast CT showing 5.5cm main artery SAA. Coeliac angiogram confirms the SAA. Embolisation using vascular plug for the front door and coils for the back door. 2months follow up arterial phase post contrast CT confirming exclusion of the aneurysm from the circulation with less than 20% infarction of the splenic parenchyma without clinical significance,yes
PMC4986551,Figure_4,oa_package/77/e0/PMC4986551.tar.gz,[],"Figure4. Immunohistochemistry in midbrain at ages 1517 months. Bright-field immunohistochemical stainings were performed in age-matched WT-F1-hybrids, PrPmtA and DM mouse (for , see lower panels) midbrain (focused on a region between VTA and SNc, with ventral surface below and midline to the left) in comparison. Protein aggregation was detected by antibodies against phospho-Serine129-alpha-synuclein (pSer129-SNCA), p62 and ubiquitin. In WT spinal cord at old age, no protein aggregates were detected. In PrPmtA, the pSer129-SNCA antibody again detected few neuronal nuclei owing to the overexpression of SNCA in the basal brain (4D, open arrow). In DM, strong immunoreactivity throughout these areas in neuronal cytoplasm with extension along neurites can be observed. In high-magnification panels (panel row below), the granular and thread-like composition of the signals and the corkscrew-appearance of some immunopositive neurites become apparent (4J, black arrows), as well as the higher sensitivity of the pSer129-SNCA antibody for this pattern.",yes
PMC4153268,Figure_7,oa_package/50/a5/PMC4153268.tar.gz,"['4])Bronchial calcification (high attenuation) was the second common finding () observed in anthracosis patients group and it was significantly more than in the control (X2=14.', 'Calcification in the bronchial wall of the right middle lobe (arrow) in an anthracofibrosis patient.']",Figure 7 Calcification in the bronchial wall of the right middle lobe (arrow) in an anthracofibrosis patient.,yes
PMC10565790,Figure_3,oa_package/66/3c/PMC10565790.tar.gz,"['R337Q Tregs also recovered FoxP3 and CD25 expression to levels closer to those of WT Tregs ( S3A); R337Q mice also included a sizable proportion of FoxP3intCD25 cells, as was already described in full FoxP3 deficiencies3,8,10,41 (A).', 'In non-lymphoid tissues, mutant males had normal Treg proportions, except for R337Q, which had an increase in gut, lungs, and skin (E), a mirror image of the paucity of tissue Tregs observed in R337Q females.', 'Finally, and perhaps most striking, was the subset distribution among colonic Tregs of R337Q males: a high proportion of Helios+ Tregs and fewer RORg+ Tregs (F) again, the exact opposite of the ratio observed in females (G).', '.']","Figure 3. Treg phenotypes in hemizygous males (A) Representative CD25/FoxP3 plots of gated CD4 TCRB Thy1.1 T splenocytes from hemizygous males; the two WT littermates shown are from B6 and B6. backgrounds. (B) Proportions of Tregs expressing the mutant FoxP3 among CD4 TCR cells in spleen from hemizygous males. (C) Representative CD44/CD62L plots of splenic Tregs from the different mutants and two WT control littermates (quantified at bottom). (D) Cumulative heatmap of the change in proportion of different markers in the splenic Treg from the mutants, each normalized to its WT littermate. (E) Proportions of Tregs expressing the mutant FoxP3 in different tissues. (F) Representative RORg/Helios plots of colonic Tregs expressing the different mutant FoxP3s or from WT control littermates (quantification from multiple experiments at right). All results are from 23 independent experiments; each dot is an individual mouse; error bars indicate mean SD; *p < 0.05, **p < 0.01, ***p < 0.0001, from Mann-Whitney test.",yes
PMC9320483,Figure_3,oa_package/72/e8/PMC9320483.tar.gz,['Representative tiles of size 1024 m 1024 m from a WSI from Center 2 that were used for evaluating the established algorithm.'],Figure 3 Representative tiles of size 1024 1024 from a WSI from Center 2 that were used for evaluating the established algorithm.,yes
PMC10493862,Figure_7,oa_package/10/58/PMC10493862.tar.gz,['MRI of the cervical spine showed an altered signal intensity involving the intervertebral disc at C3-4 with areas of T1 and T2 hypointensity consistent with calcificationMagnetic resonance examination of spine.'],"Figure 7 Magnetic resonance examination of spine. PT 33 3-year-old boy with haematoma arrow. A) T1-weighted FS sag, B) T1-weighted FS post contrast sag, C) T2-weighted sag. Mass effect extradural haematoma in spinal canal. High signal on T1- and T2-weighted imaging",yes
PMC4423886,Figure_8,oa_package/38/d0/PMC4423886.tar.gz,"['In order to find a possible explanation for this behavior, we built a model of the sequence of apoA-I involving helix 6 and 7 (A).', 'ref037"" ref-type=""bibr"">37] that replacement by Pro (in light blue in B) places a polar group in the bottom hydrophobic cluster and disrupts the native conformation around this region and -as a consequence- a significant alteration of the subtle interactions with neighboring helices that help to stabilize the entire molecule.', 'g008Model of the apoA-I peptide probably involved in the heparin binding site.', 'ref015"" ref-type=""bibr"">15] and -in addition- helix 7 contains 3 positive residues as well (Arg 171, Arg 177 and Lys 182: ).']",10.1371/journal.pone.0124946.g008,yes
PMC10286685,Figure_4,oa_package/a6/65/PMC10286685.tar.gz,"['Type of imaging being rejected, as reported by respondents MRI: magnetic resonance imaging; CT: computed tomography; PET: positron emission tomographyThis study demonstrated various situations when the test requests of the respondents were rejected by the imaging center.']","Figure 4 Type of imaging being rejected, as reported by respondents MRI: magnetic resonance imaging; CT: computed tomography; PET:positron emission tomography",yes
PMC7551280,Figure_4,oa_package/01/b2/PMC7551280.tar.gz,"['The accumulation of total, MNs and PMNs leukocytes in the intraperitoneal cavity was slightly reduced under WEB-2086 pre-treatment (A).', 'Moreover, WEB-2086 treatment did not change the production of LTB4 and PGE2, but effectively induced a decrease of LTC4 and TXB2 (B).', 'The local (C) and systemic (D) production of IL-6 was significantly reduced with WEB-2086 treatment, whereas the reduction of MCP-1 was less significant.', 'WEB-2086 treatment decreases the production of LTC4, TXB2 and IL-6 induced by BaV.']","Figure 4 WEB-2086 treatment decreases the production of LTC , TXB and IL-6 induced by BaV. One hour before i.p. BaV inoculation (0.5 mg/kg), C57BL/6 mice were s.c. treated with WEB-2086 (5 mg/kg). After selected periods of BaV inoculation, peritoneal exudate was harvested to evaluate: ( ) accumulation of total, mononuclear (MNs) and polymorphonuclear leukocytes (PMNs) after 4 h; ( ) the production of eicosanoids LTB , LTC , PGE and TXB , after 30 min; and ( ) the production of local IL-6 and MCP-1 after 4 h. ( ) Plasma samples were used to detect the production of systemic IL-6 and MCP-1 after 4 h of BaV inoculation. (*) Differences between BaV and control; (#) differences between mice pre-treated or not with WEB-2086. Results were expressed as mean SD (45 mice) of three reproducible assays. (* or #) < 0.05, (** or ##) < 0.01, (***) < 0.001 and (****) < 0.0001.",yes
PMC11384437,Figure_3,oa_package/2a/06/PMC11384437.tar.gz,"['The patient s chest CT showed a reduction in lung metastatic lesions, and cranial MRI revealed multiple abnormal signals within the skull, with some lesions showing reduction in size and significant improvement in surrounding edema zones ().', 'CT/MRI scans evaluate the progression of disease.']","Figure 3 CT/MRI scans evaluate the progression of disease. (A,B) CT scan of the thorax reveals an enlarged anterior mediastinal mass and multiple metastatic lung nodules after the several cycles of chemotherapy. (C) MRI shows brain edema and lesions after chemotherapy. (D,E) Both mediastinal and pulmonary lesions are partially reduced after four cycles of tislelizumab. (F) MRI shows brain edema and lesions after four cycles of tislelizumab. (G) MRI shows brain edema and lesions after six cycles of tislelizumab. CT, computed tomography; MRI, magnetic resonance imaging.",yes
PMC9488916,Figure_5,oa_package/4a/84/PMC9488916.tar.gz,"['Exogenous lipoid pneumonitis is a peculiar variant of aspiration pneumonitis due to prolonged aspiration of mineral oils leading to a pulmonary histological lesion identical to the subcutaneous paraffinoma with areas of dense fibrosis including clear vacuoles of different size, often surrounded by foamy macrophages (figure 5).']","FIGURE5 a) Lipoid exogenous pneumonia on bronchoalveolar lavage is characterised by macrophages with several intracytoplasmic vacuoles of various sizes; b) in chronic phases, the lesion is partially substituted by fibrosis and chronic inflammation, leading to a nodular mass known as paraffinoma.",yes
PMC3846897,Figure_2,oa_package/08/04/PMC3846897.tar.gz,"['Muscles were sectioned longitudinally in order to differentiate TUNEL positive myonuclei from non-muscle nuclei (A).', 'Both the quadriceps and gastrocnemius muscles of Nol3-/-\nSgcd\n-/- mice had significantly more TUNEL positive nuclei per fiber than Sgcd\n-/- mice (B).', 'Cleaved poly(ADP-ribose) polymerase (PARP) was elevated in Nol3\n-/-\nSgcd\n-/- muscle when compared to both Nol3\n-/- and Sgcd\n-/- (C and D).', 'Additionally, a significant increase in both cleaved caspase 8 (E and F) and caspase 3 (G and H) was detected in Nol3\n-/-\nSgcd\n-/- muscle when compared to Nol3\n-/- but not Sgcd\n-/- muscle.', 'g002Arc deficiency in Sgcd-/-Nol3-/- mice alters some markers of muscle apoptosis.']",10.1371/journal.pone.0082053.g002,yes
PMC10457148,Figure_6,oa_package/08/bd/PMC10457148.tar.gz,"['Pneumothoraces were correctly flagged by the AI algorithm (C)AI: artificial intelligence; AP: anteroposterior; COVID-19: coronavirus disease 2019Subsequent axial CT of a 76-year-old female with acute hypoxic respiratory failureSubsequent axial CT of the chest (A, B) redemonstrates pneumomediastinum (arrowheads in A) and subcutaneous emphysema (asterisk in A) with enlarging left pneumothorax (arrow in A).']","Figure 6 Subsequent axial CT of a 76-year-old female with acute hypoxic respiratory failure Subsequent axial CT of the chest (A, B) redemonstrates pneumomediastinum (arrowheads in A) and subcutaneous emphysema (asterisk in A) with enlarging left pneumothorax (arrow in A). A left-sided chest tube is also seen (arrow in B) CT: computed tomography",yes
PMC6706415,Figure_5,oa_package/4a/ed/PMC6706415.tar.gz,"[' 5a) post A injection.', ' 5a).', 'Mitigation of A toxicity in zebrafish larvae with Cas AuNPs.', 'Source data are provided as a Source Data fileMicrotome slices of the brain tissues of zebrafish larvae, treated with A and Cas AuNPs, were prepared on the fifth day post A treatment and stained with Congo red.', ' 5b), indicating elimination of A species by Cas AuNPs.', ' 5c) and polarized light microscopy (', ' 5d) further confirmed deposition of A amyloids in the brain tissues of zebrafish larvae, but not in Cas AuNPs-treated or untreated control larvae.']","Fig. 5 Mitigation of A toxicity in zebrafish larvae with Cas AuNPs. A peptide was injected into the cerebroventricular space at 10, 50, and 100fM concentrations ( =20, meanSD). Zebrafish larvae were monitored on an automated behavior monitoring system at fifth day post A treatment and parameters of total distance traveled, movement frequency and trajectory path were observed for 1h. Significant ( <0.005) difference in the behavior of the larvae was observed on the fifth day post treatment. Cas AuNPs injected, via the intracardiac route 2 and 6h after the A treatment, rescued the larvae from A toxicity and from developing Alzheimers-like symptoms. Representative trajectories of the larvae are displayed in the far-right column. Treating the larvae with Cas AuNPs, 12 and 24h post A treatment, failed to protect the larvae from developing A toxicity. Zebrafish larvae, treated with Cas AuNPs 2h after A treatment were fixed, sliced and stained with Congo red to image any A fibrils that could have formed. Tissue slices of the brain section did not present any red fluorescence, indicating no A fibril formation in Cas AuNPs treated larvae (scale bars: 200M; inset scale bar: 20M). Furthermore, immunohistochemistry (IHC) ( ) and polarized light microscopy (apple green birefringence of amyloid) ( ) revealed deposition of aggregated A in the larval brain while no A deposition was observed in Cas AuNPs or buffer treated larvae (Scale bars: IHC, 30M; polarized light microscopy, 50M). Error bars represent the standard deviation. Source data are provided as a Source Data file",yes
PMC3465941,Figure_1,oa_package/98/82/PMC3465941.tar.gz,"['The CT showed moderately dilated small bowel loops with gas within the intestinal wall ().', '0-0017114229CT abdomen and pelvis showing moderately dilated small bowel loops and pneumatosis intestinalis.']",Figure 1 CT abdomen and pelvis showing moderately dilated small bowel loops and pneumatosis intestinalis.,yes
PMC5548792,Figure_5,oa_package/ee/d3/PMC5548792.tar.gz,"[' 5a, b).', ' 5b d).', ' 5b, e).', ' 5f, g).', 'Fine-mapping of the chromosome 7 locus linked to parasitemia and host mortality.', '001\nThe 7.']","Fig. 5 Fine-mapping of the chromosome 7 locus linked to parasitemia and host mortality. Plot of peak LOD scores at the chromosome 7 locus, showing the site with peak LOD score ( ). Expanded view of the chromosome 7 locus showing candidate genes and crossover sites for three progenies with genetic recombination within the locus. Genetic markers in a 7.3kb segment has the highest LOD score defined by crossovers in progenies Cl.13 and Cl.35. Plots of the mean parasitemia with SD from three to five mice infected with three progeny as indicated. , Cytokine/chemokine levels (means and SDs) from five mice infected with the parents and three progenies, measured using a mouse magnetic 20-plex kit (Invitrogen) in a Luminex-200 machine according to the manufacturers instructions. Two-sided -test, ** <0.01; *** <0.001",yes
PMC10598507,Figure_2,oa_package/40/04/PMC10598507.tar.gz,['.'],"Figure 2. Metastasis of thyroid papillary carcinoma in the brain. Asterisk on the left shows brain parenchyma. The neoplasm exhibits papillary pattern and typical morphological nuclear features of thyroid papillary carcinoma. Hematoxylin and eosin (H&E). Magnification, objective 100.",yes
PMC4501135,Figure_6,oa_package/7c/84/PMC4501135.tar.gz,"['It is possible to do away with staining using vital dyes as the peeled ILM is well visualized as a scrolled membrane [].', 'Peeled internal limiting membrane seen as a scroll providing a visual cue of completionRolled edges of the retinal break helps localization of the lesion during surgeryRetinal detachmentIn cases of retinal detachment, MIOCT can assist during induction of posterior vitreous detachment, detection of any residual subretinal fluid at the posterior pole and completion of fluid air exchange.']",Figure 6 Peeled internal limiting membrane seen as a scroll providing a visual cue of completion,yes
PMC6782001,Figure_5,oa_package/0e/46/PMC6782001.tar.gz,"['When tested in the 20 weeks old animals, no significant tip threshold difference, in line with the ABR threshold measurement, was found between two genotypes (A, P 0.', 'Q10, also known as the sharpness of tuning and an indicator of cochlear amplifier function, was significantly reduced in the homozygotes (B, *P= 0.', 'ABR forward masking tuning curves and outer hair cell (OHC) patch clamp recordings in KI mice at 20 weeks.', 'Results of NLC measurements detected no differences (C 5H, all P 0.']","Figure 5 ( ) Homozygous mice exhibited no significant ABR threshold elevation (P>0.05, two-way ANOVA followed by Bonferroni post-test). Averaged ABR forward masking tuning curves of 11 kHz probe tone were also presented as a function of masker frequency. The averaged tip threshold of homozygous mice showed no significant difference compared to those of wild-type mice (P>0.05, Students unpaired t-test with Welchs correction). The tail portions of the FMTC overlapped between the two genotype groups. Averaged audiograms from the same tested animals were provided for reference. ( ) Significantly lower Q values measured in homozygous mice compared with their wild-type counterparts (6.228 0.3705 and 4.712 0.3353, Mean SEM for wild-type and homozygotes, respectively, *P=0.0204, Students unpaired t-test with Welchs correction). ( ) Representative OHC NLC traces from 20 week-old wild-type and homozygous mice. ( ) No significant difference was found between the Z value, V , Q and C of both genotypes (all P>0.05, Students unpaired t-test with Welchs correction). ( ) Normalized Prestins charge density Q , derived from Q /C , showing no difference between the two groups (P>0.05, Students unpaired t-test with Welchs correction).",yes
PMC4921666,Figure_5,oa_package/51/7a/PMC4921666.tar.gz,"['Examination of HE-stained sections of heart revealed mild lymphocytic myocarditis (panel A in ) at 4 8 DPI.', 'In addition to the described lesions, at 2 4 DPI, infected gulls had moderate to marked multifocal to confluent necrotizing pancreatitis (panel B in ) that was accompanied by heterolymphoplasmacytic serositis at 6 21 DPI (panel C in ', '(A) Mild lymphocytic myocarditis at 4 DPI.']",Fig. 5 (A) Mild lymphocytic myocarditis at 4 DPI. (B) Necrotizing pancreatitis at 4 DPI. (C) Heterolymphoplasmacytic serositis of the pancreas at 8 DPI. H&E stain. 40 (AC).,yes
PMC4488339,Figure_5,oa_package/be/88/PMC4488339.tar.gz,"['To determine the effect of Gal-8 treatment on the local retinal immune environment, we analyzed T cell subsets in the retina of vehicle- and Gal-8-treated mice by flow cytometry (A).', 'Compared to the vehicle-treated control mice, there was a 25% increase in Treg cell frequency and a 50% increase in Treg number in the retina of Gal-8-treated mice (A).', 'The frequency of TH2 cells was 50% higher in Gal-8-treated versus vehicle-treated retinas, which resulted in a 34% increase in total retinal TH2 cells (A).', 'There was no significant difference in the number or frequency of TH1 cells in the retinas of Gal-8-treated mice, but the frequency of TH17 cells was reduced by 37% compared to vehicle-treated mice (A).', '7-fold in the retinas of Gal-8-treated mice compared to vehicle treatment (B).', 'The anti-inflammatory cytokine IL-10 was increased by 34% in retinal extracts from the Gal-8-treated group compared to the vehicle-treated control group (C).', 'In contrast, there was a dramatic decrease in soluble retinal IFN and IL-17A in Gal-8-treated mice (C), consistent with the reduced pathology of these mice.', 'g005Galectin-8 inhibits inflammation of the retina.']",10.1371/journal.pone.0130772.g005,yes
PMC4275788,Figure_1,oa_package/2c/62/PMC4275788.tar.gz,"['He presented to a local hospital with acute pain; computed tomography (CT) imaging demonstrated a 28 cm complex cystic tumor with septal enhancement, nodularity, and inferior vena cava (IVC) compression ().', 'Undifferentiated embryonal sarcoma of the liver in an adult patient: case reportTurk J Gastroenterol23June (3)201227928322798120CT scan of primary liver sarcoma.']",,yes
PMC9076020,Figure_3,oa_package/d6/16/PMC9076020.tar.gz,[],"FIGURE 3 CFUs in the mouse superficial skin wound infection model. Mice were treated twicedaily for 3 days with vehicle only, an ATx201 dose range or licensed products Fucidin or Bactroban following inoculation with (A) MRSA 43484 (FUS and MUP susceptible), (B) MRSA 88779 (FUS resistant) or (C) MRSA 86446 (MUP resistant). The data are representative of five (A) or two (B and C) independent experiments. **** = < .0001, *** = < .001, ** = < .01, * = < .05, Dunnett's multiple comparison test. CFU, colonyforming unit; FUS, fusidic acid; MRSA, methicillinresistant ; MUP, mupirocin",yes
PMC10580452,Figure_1,oa_package/e6/a1/PMC10580452.tar.gz,[],"Figure1. Thyroid ultrasound. A, Right superior-middle pole solid isoechoic thyroid nodule (4.8 3.6 5.1cm). B, Right lower solid hypoechoic thyroid nodule (2.0 2.0 1.6cm).",yes
PMC8296685,Figure_7,oa_package/03/3e/PMC8296685.tar.gz,"[' 7A, B).', 'A, B Immunoblot images and quantification of GAPDH- normalized molecules involved in endoplasmic reticulum stress (pIRE1 /IRE1 ; pEIF2 /EIF2 ) from hippocampal extract (n = 4 6 independent samples per group).', '0001Regarding to mRNA expression, different transcript factors located downstream of IRE1 and eI2F in the UPR pathway were analyzed.', ' 7C).']","Fig. 7 , Immunoblot images and quantification of GAPDH- normalized molecules involved in endoplasmic reticulum stress (pIRE1/IRE1; pEIF2/EIF2) from hippocampal extract (n=46 independent samples per group). mRNA expression profile (n=46 independent samples per group, with 3 technical replicates per sample) of and from hippocampal extract. Statistical analysis was carried out through two-way ANOVA ( , diet variable: # denotes p<0.05) and Tukeys post-hoc where * denotes p<0.05; ** denotes p<0.01; *** denotes p<0.001 and **** denotes p<0.0001",yes
PMC7881375,Figure_11,oa_package/4f/81/PMC7881375.tar.gz,[],Figure 11 Radiographic image showing one year follow up (Case 2),yes
PMC5099138,Figure_5,oa_package/35/68/PMC5099138.tar.gz,"['Fe65 expression (A) in mean values SD and individual values for controls and patients [(A,D), respectively] and immunohistochemistry images from controls (B) and patients (C).']","Figure 5 . Patients displayed a statistically significant (*) decrease in Fe65 expression compared to controls, which indicates that Fe65 levels are lower in our patient sample. Photomicrographs show the differences in labeling between controls and patients; Fe65 expression is greater in patients than in controls , who display mild, diffuse labeling (arrow) and show nuclear expression (red arrow). Shows individual data for patients and controls: controls display increased Fe65, and all patients show values below the statistical mean. Scale bar: 50m. The dotted line indicates the significance threshold. The graphs present the percentage of cells with immunopositive inclusions in 500 .",yes
PMC10603218,Figure_1,oa_package/81/9d/PMC10603218.tar.gz,"[' Computerized tomography of the abdomen and pelvis with intravenous contrast, coronal view.']","Figure 1 Computerized tomography of the abdomen and pelvis with intravenous contrast, coronal view. Red arrow:intussusception and obstruction; white arrow: abrupt decrease in bowel caliber in the mid-ileum",yes
PMC2587915,Figure_2,oa_package/f9/b9/PMC2587915.tar.gz,"['As shown in , in situ signal was not observed in lung sections derived from mice that received intranasal administration of PBS alone (top panels).', 'However, by 3 to 4 dpi, the distribution and intensity of rMA15-specific in situ signal had greatly diminished in lung tissue of WT mice, while the rMA15-specific signal in MyD88 / mice was more intense and much more broadly distributed ().', 'g002Virus replication is sustained and distribution is more widespread within the lungs of MyD88 / mice as compared to WT mice.']",10.1371/journal.ppat.1000240.g002,yes
PMC8598118,Figure_2,oa_package/7c/f5/PMC8598118.tar.gz,"[' shows an axial image of the bladder tumour.', '\n\nAn axial image of the bladder tumour.']",Figure 2 An axial image of the bladder tumour.,yes
PMC8576671,Figure_3,oa_package/ee/47/PMC8576671.tar.gz,['A benign lymph node in Group 4R.'],"Figure 3 A benign lymph node in Group 4R. PET/CT image showed a node with FDG uptake higher than background with holistic high density (arrow). Criterion 1 diagnosed as malignant while Criterion 2 as benign. Histological section showed extensive collagenous fibrous tissue deposition surrounded by proliferating fibroblasts (haematoxylin-eosin stain; original magnification, 100).",yes
PMC9305576,Figure_3,oa_package/8b/cb/PMC9305576.tar.gz,['\nTumor.'],"Figure 3 A: The tumor was outside the anus; B: The tumor was removed surgically, in addition to about 3 cm of the affected bowel.",yes
PMC7387399,Figure_5,oa_package/ad/c3/PMC7387399.tar.gz,"['5a, b) [193].', 'uPAR in inflammatory bowel disease.']",Fig. 5 uPAR in inflammatory bowel disease. Macrophage uPAR D1-D3 expression plays a significant role in the bacterial removal while ( ) in inflammatory bowel disease macrophage differentiation is altered with as consequence an increase in uPAR D2-D3 expression and inadequate microbial maintenance. uPAR is represented by the red 3-domain structure as described in Fig. on the cell membrane of uPAR expressing cells,yes
PMC10412971,Figure_2,oa_package/df/f4/PMC10412971.tar.gz,"['Among the various IHC markers, AI-based interpretation of PD-L1 is important in the histopathologic diagnosis of GC ().', 'Representative image of DL-based PD-L1 CPS quantification from PD-L1 IHC slides of gastric tubular adenocarcinoma.']","Fig. 2 Representative image of DL-based PD-L1 CPS quantification from PD-L1 IHC slides of gastric tubular adenocarcinoma. PD-L1 IHC exhibits heterogeneous immunoreactivity by tumor and mononuclear cells, with (A) CPS <1, (B) CPS 149, and (C) CPS 50. The DL-model can distinguish PD-L1 positive (purple dot) vs. negative (sky-blue dot) tumor cells and PD-L1 positive (yellow dot) vs. negative (red dot) lymphoplasma cells (D-F). DL = deep learning; PD-L1 = programmed death-ligand 1; CPS = combined positive score; IHC = immunohistochemistry.",yes
PMC5034810,Figure_1,oa_package/bc/fa/PMC5034810.tar.gz,['Congo red staining and polarized microscopic examinations of sural nerve and skin biopsies from patients with familial amyloid polyneuropathy (FAP).'],"Figure 1 Congo red staining and polarized microscopic examinations of sural nerve and skin biopsies from patients with familial amyloid polyneuropathy (FAP). Sections of sural nerve (A, B) and skin (CF) biopsies were stained with Congo red (A, C, and E) and observed under a polarized microscope (B, D, and F). (A, B) Congo redstained materials in an arteriole of the sural nerve exhibited applegreen birefringence under polarized microscopy. (C, D) Secretory coils of sweat glands were stained with Congo red but did not show birefringence under polarized microscopy. (E, F) In a dermal nerve bundle from an FAP patient, Congo red materials were detected surrounding a small arteriole , which showed birefringence under polarized microscopy. Scale bar=50m for A and B, 200m for C and D, and 25m for E and F.",yes
PMC8525841,Figure_7,oa_package/12/20/PMC8525841.tar.gz,"['When the model eye was photographed with the Vistaro (', '.']","Figure 7. ( ) A model eye captured with a FOV of up to 70 using the Vistaro. The concentric arcs mark an increase in measurement by 10, with the first one corresponding to 30. ( ) Real eye single-shot image captured on the Vistaro showing a FOV of up to 65. The measured FOV of a single-shot image on the Vistaro was 56.36 compared with that of a single-shot image from the EIDON ( ).",yes
PMC9780325,Figure_6,oa_package/13/ab/PMC9780325.tar.gz,"[' 6.', ' 6d).', ' 6e).', ' 6f), and lower mineralization volume ratios of lung lesions (HIMB: 5%, 95% CI 2 12, P 0.', ' 6g).', 'Lung pathology assessed by computed tomography (CT) of intratracheally formalin perfused lungs.', 'In pulmonary LN, the median of TB lesions volumes was also significantly higher in the unvaccinated group (51.']","Figure 6 Lung pathology assessed by computed tomography (CT) of intratracheally formalin perfused lungs. Example of the images obtained from the lungs of an unvaccinated control and two animals vaccinated with HIMB and HIMC, respectively. ( )Larger images on the left show the tracheobronchial tree and the TB lesions. ( ) The top right miniatures show the TB lesion (whitish) superimposed onto the lung volume (yellowish). ( ) The bottom right miniatures show (in red) the mineralized portion of the TB lesion. ( ) Volumes of lung TB lesions in each group expressed in cm . ( ) To evaluate the lesion dispersion within the lungs in each group, the number of lobes with TB lesions in each animal is plotted against the number of animals. The control group has a higher number of animals with a higher number of affected lobes than the vaccinated groups. ( ) Volume of lesion mineralization expressed in cm . ( ) Ratio between the volume of mineralization and the volume of lung lesions expressed in %. Horizontal lines in ( ), ( ) and ( ) represent the median values. Groups: Control (n=7, green), HIMB (n=7, blue), HIMC (n=7, red). * <0.05, ** <0.01, KruskalWallis test followed by one-tailed Dunns test for multiple comparisons.",yes
PMC5396526,Figure_3,oa_package/00/f5/PMC5396526.tar.gz,"['To put the extent of glucocorticoid excess into context, we compared glucocorticoid output in the primary aldosteronism patients to that in patients with adrenocortical adenomas that were either endocrine inactive or fulfilled the diagnostic criteria for subclinical and clinically overt Cushing s syndrome (, SI Appendix, and Supplemental Table 2).', 'A detailed pathway analysis of the steroid metabolome in the 4 different disease groups revealed distinct differences (SI Appendix and Supplemental ).', 'Their androgen production largely resembled those in controls but showed an increased excretion of the major adrenal androgen metabolite 11 -hydroxyandrosterone (SI Appendix and Supplemental A).', 'This was found in primary aldosteronism, but none of the other groups (D).', 'By contrast, the overt Cushing s group (SI Appendix and Supplemental B) showed decreased androgen output in the presence of increased glucocorticoid and mineralocorticoid precursor metabolites.', 'The subclinical Cushing s groups (SI Appendix and Supplemental , C and D) showed a similar pattern to overt Cushing s, albeit attenuated with only 2 of 9 glucocorticoid metabolites significantly increased.', 'Patients with primary aldosteronism and overt Cushing s patients showed significant increases in all 9 glucocorticoid metabolites (SI Appendix and Supplemental , A and B).', 'Steroid metabolite excretion in primary aldosteronism in comparison to healthy controls and patients with endocrine-inactive and cortisol-producing adrenal adenomas.']","Figure 3 Steroid metabolite excretion in primary aldosteronism in comparison to healthy controls and patients with endocrine-inactive and cortisol-producing adrenal adenomas. The panels show the 24-hour urinary excretion of tetrahydroaldosterone ( ), cortisol ( ), total glucocorticoid metabolites ( ), and the major adrenal androgen metabolite 11-hydroxyandrosterone ( ) in primary aldosteronism patients (PA; = 174) in comparison to healthy controls (Co; = 162), patients with endocrine-inactive adrenal adenoma (EIA; = 56), patients with subclinical Cushings (differentiated into 2 groups: SC1 ( = 55), morning cortisol after 1 mg dexamethasone overnight > 50 and < 138 nmol/l; SC2 ( = 49), morning cortisol >138 nmol/l), and overt adrenal Cushings syndrome patients (Cu; = 47). Boxes represent median and interquartile range, whiskers represent 5th and 95th centiles. ** < 0.01 versus controls, *** < 0.001 versus controls. Comparisons between groups were made with linear regression models to adjust for age and sex in comparisons between all 6 groups.",yes
PMC3691657,Figure_5,oa_package/e0/14/PMC3691657.tar.gz,['Evidence of S-nitrosylation in HCC: Increased S-nitrosylation is evident in the fibrous portion and tumor cells in HCC.'],Figure 5 Anti SNO-Cys antibody was used to reveal the distribution of S-nitrosylation signals in the HCC and controls. Significant increase CYB5A is also evident in both fibrous and tumor cells similar to S-nitrosylation. Figure & represents control liver on the other side explained strong S- nitrosylation signals in HCC.,yes
PMC9977490,Figure_2,oa_package/7e/b8/PMC9977490.tar.gz,"['The PF-07321332, but not MK-4482, monotherapy treatment group s viral load was also significantly lower at 4 dpi (A).', 'By 2 dpi, the differences between treated and untreated animals were increased, and a larger subset of animals in each treatment group had no detectable infectious virus (B).', 'There were no significant differences in the levels of viral RNA detected in the oral swabs at any time point examined (C), but AUC analysis performed for the entirety of the study revealed a significant difference between the combination therapy and the vehicle control (Supplemental A; supplemental material available online with this article; Immunohistochemistry analysis with 3 different markers (400x magnification) showed strong cytoplasmic expression of Cytokeratine (a), which was as strong as Vimentin (b), Meanwhile, S100 demonstrated a slightly weaker expression (c), (inset : 200x magnification).']","Figure 3 Immunohistochemistry analysis with 3 different markers (400x magnification) showed strong cytoplasmic expression of Cytokeratine (a), which was as strong as Vimentin (b), Meanwhile, S100 demonstrated a slightly weaker expression (c), (inset : 200x magnification).",yes
PMC5526151,Figure_2,oa_package/33/77/PMC5526151.tar.gz,"['Neurons derived from the control SAMR1 group appeared to be normal with slender and smooth projections (Aa) whereas, neurons extracted from SAMP8 were in a poor state, with fragmented or bead-like processes (', '5 M rapamycin, a well-known mTOR inhibitor, the neurons from SAMP8 exhibited an improved appearance when compared with the non-treated SAMP8 group, with smooth and slender projections in some of the neurons (Ac).', '0 M rapamycin, the neurons exhibited a poorer cell state when compared with the untreated SAMP8 group, with most neurons lacking projections (Ad).', '05; B).', '.']","Figure 2. Rapamycin treatment may promote cell morphology and alleviate the Tau phosphorylation of neurons from SAMP8. (A) Cell morphology (magnification, 200). The neurons derived from SAMR1 appear to be in a normal state (a, Neurons-SAMR1). The neurons extracted from SAMP8 were in poor state with most processes broken and exhibited bead-like changes (indicated by the white arrows) (b, Neurons-SAMP8). When pretreated with 0.5 M rapamycin, neurons from SAMP8 were partially improved with some smooth and slender processes (indicated by the white arrows; c, Neurons-SAMP8 + 0.5 M rapamycin). When pretreated with 1.0 M rapamycin, the cell state was worse than the untreated group and most neurons lacked projections (d, Neurons-SAMP8 + 1.0 M rapamycin). (B) Western blot analysis was used to investigate Tau phosphorylation. In the neurons-SAMP8 group, the protein expression levels of Tau (pS199) and Tau (pS396) were significantly increased when compared with the control neurons-SAMR1 group. When pretreated with 0.5 M rapamycin for three days, Tau (pS199) and Tau (pS396) exhibited significantly decreased levels of protein expression when compared with the neurons-SAMP8 group. *P<0.05 vs. neurons-SAMP8 + Rapamycin group; P<0.01 vs. neurons-SAMP8 group. SAMP8, senescence accelerated mouse prone 8; SAMR1, senescence accelerated mouse resistant 1. SAMP8 group, brain tissue of SAMP8; SAMR1 group, brain tissue of SAMR1; neurons-SAMP8 group, neurons of SAMP8; neurons-SAMR1 group, neurons of SAMR1.",yes
PMC10789373,Figure_58,oa_package/bf/e5/PMC10789373.tar.gz,[],"Figure 8.2 Longitudinal 2D strain assessment in the right ventricle of a patient with cardiac transthyretin amyloidosis, showing a lower absolute global value (RVGLS = 9.9%) and an absolute reduction in mean free wall strain (FWS) (11.4%), with lower values in middle and basal segments and higher values in apical segments. TAPSE: tricuspid annular plane systolic excursion.",yes
PMC11524542,Figure_1,oa_package/46/2b/PMC11524542.tar.gz,"['Images A and B show axial magnetic resonance imaging (MRI) scans from an examination of an 82-year-old male with no acute abnormalities, demonstrating age-related involutional changes.']","Figure 1 Images A and B show axial magnetic resonance imaging (MRI) scans from an examination of an 82-year-old male with no acute abnormalities, demonstrating age-related involutional changes.",yes
PMC11616529,Figure_4,oa_package/82/b9/PMC11616529.tar.gz,"['Medium sized neoplastic cells with epithelioid/rhabdoid morphology on hematoxylin/eosin staining (left, x400).', 'The patient was referred to an oncology department where she underwent whole neuraxis, chest/abdominal CT imaging for disease staging (negative for secondary foci) and received combined chemotherapy with cisplatin and etoposide, and whole brain radiotherapy with VMAT protocol of 60 Gy in 30 fractions.']","Fig. 4 Medium sized neoplastic cells with epithelioid/rhabdoid morphology on hematoxylin/eosin staining (left, x400). Immunostaining (right, x400) showing typical loss of nuclear expression of SMARCB1/INI-1 in the neoplastic cells. Retain of the protein expression in the endothelial cells (positive internal control).",yes
PMC6170493,Figure_5,oa_package/0b/b3/PMC6170493.tar.gz,"[' 5b).', ' 5b).', ' 5c).', 'Involvement of ALS proteins in response to hypoxic insult.', 'DNA damage repairBased on the canonical pathways that are highlighted as significantly covered by ALS proteins, maintaining the stability of DNA appear to be an important task.']","Figure 5 Involvement of ALS proteins in response to hypoxic insult. ( ) Bar graphs of canonical pathways involved in response to hypoxic insult, as highlighted by ALS protein interactions (p(log value)) and their overlap (ratio, yellow line). ( ) Image of NRF-2 mediated oxidative stress response pathway, representing the extent of ALS protein involvement. ALS proteins with higher binding partners are marked with increasing color intensity. ( ) Circular representation of ALS proteins that are commonly present among different canonical pathways.",yes
PMC3188523,Figure_1,oa_package/72/4c/PMC3188523.tar.gz,"['mansoni, MOLF mice were debilitated, as defined by hunched posture, reduced activity and scruffy appearance, and exhibited significantly enhanced hepatic egg-induced immunopathology when compared with BL/6 mice, with granulomatous lesions in some instances larger than those seen in the high-pathology control CBA mice (\nA\n).', 'Individual granulomas from MOLF mice consisted of significantly larger perioval aggregates of macrophages/histiocytes and lymphocytes admixed with eosinophils and neutrophils, with a greater tendency to infiltrate the surrounding hepatic parenchyma than in BL/6 mice (\nB\n).', 'However, the number of schistosome eggs present in the livers did not significantly differ between the mouse groups, indicating that the parasite load did not correlate with the extent of pathology (\nC\n).', 'Analysis of antigen specific cytokine production by schistosome egg antigen (SEA)-stimulated draining mesenteric lymph node (MLN) cells (MLNC) from infected mice revealed that MOLF mice produced strikingly higher amounts of IL-17 than BL/6 and even CBA mice (\nD\n).', 'There also was a significant, but less pronounced, increase in IFN- , IL-6 and TNF (\nE G\n).', 'However, there were no significant differences in IL-4, IL-5 or IL-10 between BL/6, CBA and MOLF mice (\nH J\n).', 'g001Schistosome-infected wild-derived MOLF mice develop severe egg-induced immunopathology and a proinflammatory cytokine profile.', 'Similar to the MLNC, MOLF GC produced very high amounts of IL-17 compared with both BL/6 and CBA mice (\nK\n).', 'BL/6 mice, but only in the case of TNF was the difference statistically significant (\nL N\n).', 'Analysis of cytokines involved in the development of Th17 cells revealed that IL-1 , as well as IL-23p19 and IL-12p40, the subunits that make up IL-23, were expressed at much higher levels in the livers of MOLF mice than BL/6 and CBA mice (\nO Q\n), whereas the difference in the IL-12-specific subunit IL-12p35 was less striking (\n']",10.1371/journal.ppat.1002272.g001,yes
PMC7492852,Figure_2,oa_package/81/8f/PMC7492852.tar.gz,"[' 1More so, due to the large surface area of nanomaterials, they can be employed as site directed targeted therapy in cancer when functionalized or conjugated with peptides, antibodies, DNA or RNA strands, aptamers and other small molecules ( 2).', ' 2Schematic diagram showing the mechanisms of action of targeted functionalized nanoparticulate system.']","Figure2 Schematic diagram showing the mechanisms of action of targeted functionalized nanoparticulate system. The anticancer drug is loaded into the nanoparticles, and the surface of nanoparticles is engineered with cell-specific ligand that can recognize and target cancerous cell. The engineered nanoparticles then localize and binds to the receptors found on the surface of cancer cell, thereby resulting in internalization of the nanoparticles by endocytosis. Inside the cancerous cell, the nanoparticles undergo endosomal escape, which lead to the release of the drug for its cytolytic activity that causes cell death [ ].",yes
PMC11247583,Figure_1,oa_package/7c/1e/PMC11247583.tar.gz,"['Additionally, a soft tissue mass was detected in the left sphenoid sinus-floor/ethmoid sinus region with a maximum diameter of approximately 30 mm ().', 'The coronal (A), sagittal (B), and axial (C) planes of pre-operative brain and pituitary MRI with GBCAs.']","Figure 1 The coronal (A), sagittal (B), and axial (C) planes of pre-operative brain and pituitary MRI with GBCAs. It showing a small nodular abnormal signal on the left side of the pituitary, approximately 7 mm in diameter, and a cystic-solid mixed soft tissue mass in the left sphenoid sinus and anterior cranial fossa, with a maximum diameter of approximately 3 cm. MRI, magnetic resonance imaging; GBCAs, gadolinium-based contrast agents.",yes
PMC5968118,Figure_2,oa_package/c7/37/PMC5968118.tar.gz,"['Comparison between (A) total alpha-synuclein ( -syn), (B) exosomal -syn, (C) exosome concentration, ratio of exosome-associated -syn to (D) exosome concentration and (E) total -syn in controls and PD patients.']","Figure 2 Comparison between total alpha-synuclein (-syn), exosomal -syn, exosome concentration, ratio of exosome-associated -syn to exosome concentration and total -syn in controls and PD patients. Exosomal/total -syn ratio and disease severity correlation. ** < 0.01, *** < 0.001 Students -test.",yes
PMC6335931,Figure_4,oa_package/4f/0e/PMC6335931.tar.gz,"['Histologically, PMAs display a marked myxoid matrix and monomorphous bipolar cells that often radiate from vessels in an angiocentric pattern ().', 'Histological features of PMA.']","Figure 4 Histological features of PMA. Monomorphous bipolar tumor cells in an expansive myxoid matrix (H&E, original magnification 200) (a,b). Tumor cells arranged in a radiating angiocentric pattern around vessels (H&E, original magnification 400) (c).",yes
PMC4651348,Figure_5,oa_package/e4/fe/PMC4651348.tar.gz,"['StimuliA total of 40 region-of-interest images cropped from anonymized mammograms approved for research use by the University of Arizona IRB, consisting of 20 samples with malignant masses and 20 samples with benign masses were used (see for representative images).', 'g005Examples of benign (left) and malignant (right) masses in mammograms.', 'Experiment 3: Mammograms with massesAs can be appreciated by scrutinizing the representative images depicted in , discriminating benign from malignant masses is very challenging indeed; the distinguishing features are extremely subtle.', 'In contrast, evaluating the malignant potential of detected breast masses () can be very challenging [B) stages in MuLV infection when immune pathology partially or fully manifests IDO1 transcripts were detected exclusively in sorted spleen cells expressing CD11c and CD19 (CD19+ DCs).', 'At early times in MuLV infection (28dpi), ecotropic helper (GAGEco) and pathogenic (GAGDef) MuLV retrovirus genes were transcribed at high levels in sorted cells expressing neither CD19 or CD11c (CD11cnegCD19neg) and to lesser extents in CD19+ DCs, relative to sorted conventional DCs and B cells (A).', 'At later stages in infection (56dpi), GAGDef transcription was still relatively high in CD11cnegCD19neg and CD19+ DCs, though GAGEco transcription was relatively high in conventional and CD19+ DCs but not in CD11cnegCD19neg cells (B).', 'Kyn levels increased significantly in cultures containing sorted CD19+ DCs and OT-2 T cells but Kyn was not detected in cultures containing sorted DCs alone, or sorted DCs cultured with OT-1 T cells (C).', 'Consistent with these findings, anti-CD4 mAb treatments to deplete CD4 cells in vivo reduced IDO activity (D) and IDO1 transcription (E) in spleen to basal levels observed in na ve mice.']",10.1371/journal.ppat.1005615.g002,yes
PMC11554683,Figure_3,oa_package/2a/17/PMC11554683.tar.gz,"['3A).', 'Elevated GNG5 levels aggravate amyloid pathology in 5 FAD and FAD4T mice.', 'GNG5@EVRVG was secreted and enriched from supernatant of 293T-RVGOE-GNG5OE simultaneously overexpressing RVG-Lamp2b and GNG5, the cell was constructed by sequentially stabilizing transfection of pcDNA GNSTM-3-RVG-10-Lamp2b-HA vector and LvCP06-GNG5 vector (', '3A).', '3B and S5C).', '3A, C).', '3B), and particle diameters (', '3D) and hippocampus (', '3E, F) and hippocampus (', '3E, F and S5G, H).', '3G, H).', '3I, J).', '3A).', '3, 4).', 'tif"">Supplementary \n\n.']",Figure 1. (a) Anteroposterior and (b) lateral plain radiographs showing an osteolytic lesion with a pathological fracture in T5,yes
PMC7315981,Figure_11,oa_package/34/74/PMC7315981.tar.gz,[],"Fig. 6b Convex transducer; small subpleural consolidation () and a vertical artifact behind the bottom of the lesion (the so-called: C line, ).",yes
PMC3465761,Figure_4,oa_package/02/5f/PMC3465761.tar.gz,"[""An example of the images from 'unrecognizable, too poor for diagnosis' group.""]","Fig. 4 An example of the images from 'unrecognizable, too poor for diagnosis' group.",yes
PMC11241908,Figure_6,oa_package/ae/4d/PMC11241908.tar.gz,"[' 6).', '\nProposed model for Lin Body interaction with glia.', 'Finally, microglia and peripheral macrophages are recruited to restore homeostasis, resulting in an increased population of IBA1+ cells surrounding pTDP-43+ vessels (G)\nIn a healthy brain, astrocytes have many functions, including maintaining the blood-brain barrier (BBB) and coordination of the cerebral blood flow.', ' 6).']","Fig. 6 Proposed model for Lin Body interaction with glia. This figure illustrates our hypothesis for the potential sequala occurring with Lin Bodies and the resulting glial changes observed in and around capillaries throughout the brain. Vascular pathologies, such as arteriolosclerosis, occur in neurodegenerative diseases such as LATE-NC. However, it is now understood that TDP-43 within blood vessels is also associated with vascular changes, including breakdown and increased permeability of the BBB, resulting in TDP-43 leakage and microhemorrhages . In response, reactive astrocytes attempt to sequester leaking TDP-43, resulting in localization within the endfeet . Following intracerebral hemorrhaging, processes such as erythrophagocytosis lead to increased intracellular levels of iron and iron storage proteins, including ferritin . Once internalized, TDP-43 and increased levels of iron lead to astrocyte morphological changes without an apparent increase in the number of cells . Typically, cerebral vasculature is covered by astrocytic endfeet, which may represent a first response to leakage of vascular TDP-43. Nonetheless, should astrocytes become incapable of an adequate response, we might observe an accumulation of perivascular Lin bodies not colocalizing with either GFAP or IBA1. . Finally, microglia and peripheral macrophages are recruited to restore homeostasis, resulting in an increased population of IBA1 cells surrounding pTDP-43 vessels",yes
PMC6058401,Figure_5,oa_package/92/b9/PMC6058401.tar.gz,"['3) through the septal perforation ( 5, Video 5, Video 6).', ' 5Cardiac magnetic resonance imaging of the LVA, site of left ventricular (LV) rupture, and communication with the right ventricle on a right ventricular (RV) stack image (A) and two-chamber stack (B).']","Figure4 Cardiac computed tomography of the LVA communicating with the right ventricle (RV) and its location in relation to the LAD stent on off-axis imaging . , Left ventricular.",yes
PMC6812745,Figure_5,oa_package/d3/e8/PMC6812745.tar.gz,"['ref026"" ref-type=""bibr"">26] Germinal centers demonstrated normal architecture in syngeneic and untreated groups but was completely disrupted in APRIL/BLyS blockade treated animals ().', 'g005Spleen PAX5 significantly decreased in APRIL/BLyS blockade treated mice.']",10.1371/journal.pone.0223889.g005,yes
PMC5036965,Figure_7,oa_package/18/61/PMC5036965.tar.gz,['10.'],10.7554/eLife.17047.010,yes
PMC11524894,Figure_7,oa_package/e5/a0/PMC11524894.tar.gz,"['Red arrows represent identified best risk stratification cutoffs for corresponding parameters used later for prognostic analyses ( 7).', 'Importantly all four parameters show independent prognostic value (when analyzed together with common pathological variables pT and pN) for cancer-specific and progression-free survival (PFS), for both LUAD (s 7A 7H) and LUSC (s 7I 7P).', 'As for TLS-TD, it allows for the identification of a smaller subset of patients with unfavorable outcomes in LUAD (TLS-TD low) and with favorable outcomes in LUSC (TLS-TD high) (s 7A 7E, 7I, and 7M).', 'Notably, the combination of the two density parameters (TLS-TD, NECR-TD) either as a direct ratio (T/NR) or as a stratification measure (T + NR; PG 1 3) allows for even better stratification of patients with good, moderate, and poor prognosis, which is independently valuable for prognosis estimation with cancer-specific survival (CSS) and PFS as endpoints in multivariate Cox analysis (s 7C, 7D, 7G, 7H, 7K, 7L, 7O, and 7P).', ' 7Evaluation of prognostic role of AI-based prognostic parameters for cancer-specific and progression-free survival endpoints(A H) Lung adenocarcinoma cohort: (A and E) TLS-TD, (B and F) NECR-TD, (C and G) T/NR, (D and H) [T + NR].', 'However, different thresholds might be necessary for these subtypes reflecting a differing biology and aggressivity ( 7D).', 'To the best of our knowledge, we present the first AI-based algorithm for necrosis density quantification in lung cancer and show its independent prognostic value in LUAD (PFS, CSS, OS) and LUSC (CSS; PFS and OS statistical trend) (s 7 and S13).', 'Moreover, in combination with TLS ( 7), either as a direct T/NR ratio or using a cumulative prognostic grouping (T + NR), necrosis appears as a valuable marker for prognostic patient stratification in both LUAD and LUSC.']","Figure6 Development of AI-based, quantitative prognostic parameters (A) AI-based quantification of tertiary lymphoid structure (TLS) density. Three lung cancer cases are shown with yellow regions corresponding to detected TLS objects. Other colors as in B. (B) AI-based quantification of intratumoral necrosis density. Three lung cancer cases are shown with navy blue regions corresponding to detected necrosis regions. Other colors as in B. (C) Principle of quantification for four new prognostic parameters: TLS tumor density (TLS-TD), necrosis tumor density (NECR-TD), TLS/necrosis ratio (T/NR), and cumulative score with three prognostic group based on combination of TLS-TD and NECR-TD. T/NR and cumulative scores are compound parameters based on TLS and NECR quantification in the tumor. The parameters are fully explainable and do not involve any black box features from deep learning algorithm. The parameters TLS-TD, NECR-TD, and T/NR are dichotomized using identified optimal cutoff to derive prognostic subgroups. (D) Distribution of TLS-TD, NECR-TD, and T/NR values among LUAD and LUSC cases of the prognostic test cohort. In each plot each measurement is a case-level value of the corresponding parameter. TLS-TD for LUAD/LUSC and NECR-TD for LUSC are linearly upscaled using x1,000 multiplication (NECR-TD for LUSC using x100) for easiness of perception. Red arrows represent identified best risk stratification cutoffs for corresponding parameters used later for prognostic analyses ( ). See also .",yes
PMC7533078,Figure_1,oa_package/79/62/PMC7533078.tar.gz,"['The authors experience at a tertiary care hospital in New Delhi suggests that there is a set of typical findings on MRI which results in a differential of IGM, tuberculous mastitis, or malignancy most particularly the finding of extensive NME with areas of clustered ring-like pattern [].', '[28]:T1 post-contrast subtraction magnetic resonance images with clustered ring enhancement of differing etiologies (arrows).']","Figure 1: T1 post-contrast subtraction magnetic resonance images with clustered ring enhancement of differing etiologies (arrows). (a) 35-year-old female with palpable left breast lump post-fine- needle aspiration at an outside hospital, found to have idiopathic granulomatous mastitis. (b) 39-year-old female with palpable right breast lump and bloody discharge, as well as prior history of uterine tuberculosis, found to have infiltrating ductal carcinoma. (c) 31-year-old female with enlargement of the left breast 1 year after ceasing lactation, found to have tuberculous infection of the breast.",yes
PMC11577142,Figure_1,oa_package/28/83/PMC11577142.tar.gz,['Transverse view CT depicting the appendix fistulized to the iliopsoas accompanied by abscess formation.'],Figure 1 Transverse view CT depicting the appendix fistulized to the iliopsoas accompanied by abscess formation. White dotted line: border of the appendix. Red line: border of the iliopsoas.,yes
PMC3769355,Figure_1,oa_package/00/81/PMC3769355.tar.gz,"['The liver of p19 / and p35 / mice, however, demonstrated less portal inflammation and biliary cell damage compared with WT mice (A and 1B).', 'These studies indicate the presence of both CD4+ and CD8+ T cells infiltrate in affected portal tracts but there was no detectable difference in the phenotype of cellular infiltrates between the WT and IL-23p19 / mice (C).', 'g001IL-12p40 is required for 2OA-BSA-induced cholangitis.']",10.1371/journal.pone.0074225.g001,yes
PMC10700051,Figure_11,oa_package/ec/cd/PMC10700051.tar.gz,[],Figure 11 Posterior disocclusion (canine-guided occlusion) on lateral eccentric movements and protrusion.,yes
PMC11573172,Figure_1,oa_package/0f/bb/PMC11573172.tar.gz,"['ESI 1 or 2 category patients who were 18 years of age or older, who were not pregnant, and who had not received cardiopulmonary resuscitation were included in the study [].', 'tjem_45_24-f001"">Flowchart.']",Figure 1 Flowchart. ESI: Emergency Severity Index,yes
PMC2831459,Figure_3,oa_package/b0/fb/PMC2831459.tar.gz,"['Histological section showed a papillary tumor with fibrovascular cores covered by uniform cells with granular apical accentuation characteristic of acinar cell carcinoma ().', '002""/>Photomicrograph showing papillary projections lined by cuboidal cells.']",Figure 3 Photomicrograph showing papillary projections lined by cuboidal cells.,yes
PMC8714048,Figure_6,oa_package/6a/c5/PMC8714048.tar.gz,['Diagnosis of carcinoma of the lung with tumour markers.'],Figure 6 Diagnosis of carcinoma of the lung with tumour markers. A: Cytokeratin 7 positive lung carcinoma.B: Cytokeratin 20 negative lung carcinoma.C: Thyroid transcription factor 1 positive lung carcinoma.,yes
PMC6879761,Figure_2,oa_package/6e/de/PMC6879761.tar.gz,"['2a, b) suggesting that TNF may modulate the intestinal stem cell niche in different ways within the proximal SI compared with other intestinal regions.', 'TNF causes Paneth cell degranulation in proximal SI but does not change the rate of cell proliferation.', 'Proliferation in the SI in vivo occurs within the transit-amplifying region of the crypt; a distinctly different part of the crypt-villus axis to the villus tip, which is the region most susceptible to apoptosis in response to systemically administered TNF.', '2c, d).', 'png"">Supplemental \n\nA 49-year-old with bleeding and fowl smelling vaginal discharge was referred with clinically suspected carcinoma cervix.']","Fig. 8 A 49-year-old with bleeding and fowl smelling vaginal discharge was referred with clinically suspected carcinoma cervix. Biopsy revealed grade 3 endometroid endometrial carcinoma and molecular profile showed p53 abnormality. Magnetic resonance imaging high-resolution T2 weighted ( ) sagittal and ( ) axial and ( ) diffusion-weighted imaging images with apparent diffusion coefficient map showed polypoidal intermediate signal intensity growth lower uterus and cervix infiltrating the cervical stroma, vagina, and the left parametrium, and associated with a large hydrometra. There was no evidence of metastases on computed tomography (not shown). ( ) Histopathology slides of immunohistochemistry tests show positive staining for ( ) estrogen receptors, ( ) progestin receptors and ( ) vimentin, thus proving endometrial origin of the tumor and was also positive for p53 mutation ( ). The patient underwent neoadjuvant chemotherapy followed by radical hysterectomy and adjuvant radiotherapy.",yes
PMC5391900,Figure_1,oa_package/c6/1c/PMC5391900.tar.gz,"['The CDS intervention consisted of four successive screens to capture data to determine the utility of the study according to the OAR (A D), and one educational screen (E).', 'Multicentre Ankle Rule Study GroupBMJ1995311700559477663253A EClinical decision support screens for the Ottawa ankle rule integrated in the computerized physician order-entry system.']",Figure 1AE Clinical decision support screens for the Ottawa ankle rule integrated in the computerized physician order-entry system. emergency department,yes
PMC3636968,Figure_2,oa_package/7b/a4/PMC3636968.tar.gz,['Hypercellular lamina propria with mast cells infiltration.'],Fig. 2 Hypercellular lamina propria with mast cells infiltration.,yes
PMC6014451,Figure_3,oa_package/5d/4c/PMC6014451.tar.gz,['Running exercise reduces expression of caspase 1 and IL 1 in a coronary vessel.'],"Figure 3 Running exercise reduces expression of caspase1 and 1 in a coronary vessel. Dual fluorescence combining caspase1 (red, ad) or 1 (red, ad) with (green, eh) with the use of specific antibodies against followed by fluorescentlabeled secondary antibodies in mouse coronary arterioles (A and C). Merged images (il) show the expression of 1 (red) or caspase1 (red) in coronary arterioles (A and C). Quantification graphs display protein expression of caspase1 and 1 in the whole coronary vessel and endothelial cells, showing that running exercise reduced induced 1 expression in coronary vessels and endothelial cells (B and D). Data shown are representative of three separate experiments. =9 field of view/three animals/group. * < 0.05 versus ; < 0.05 versus . IL, interleukin; LFSED, lowfat diet with sedentary; HFSED, highfat diet with sedentary.",yes
PMC9309237,Figure_1,oa_package/69/6f/PMC9309237.tar.gz,"['1a).', 'Generation and characterization of VSV-vectored COVID-19 vaccine candidates.', 'BHK-G43 cells are transgenic BHK-21 cells, which express the VSV G protein in a regulated manner17.', '1b).', '1c, -G/M blot).', '1c, -S blot).', '1c, -S blot).', '1d, left panels).', '1d, central panels).', ' 1d, right panels).', '1a).', '1c, d).']","Fig. 1 Generation and characterization of VSV-vectored COVID-19 vaccine candidates. Genome maps of recombinant VSV vectors. The original VSV contains five transcription units encoding the N, P, M, G, and L genes. The VSV vector was modified by replacing the G gene with a modified SARS-CoV-2 spike gene and by inserting an additional transcription unit encoding GFP. M denotes a quadruple mutant M gene encoding an M protein that lacks host shut-off activity. G denotes a synthetic membrane protein presenting the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein. Virus yield on Vero E6 and BHK-G43 cells. Vero E6 cells, BHK-G43 cells expressing the VSV glycoprotein (+), and BHK-G43 cells lacking VSV glycoprotein expression () were infected with either VSV*M G-S (S ), VSV*M G-S (M -S ), or VSV*G-G (G ) using 0.1 ffu/cell. At the 24hours pi, infectious virus released into the cell culture was titrated on Vero E6 cells. Mean values and standard deviations of 3 infection experiments are shown. Western blot analysis of recombinant VSV vector particles. VSV*G-S (S ) and VSV*M G-S (M -S ) were propagated on both Vero E6 and BHK-G43 cells, while VSV*G-G (G ) were propagated only on BHK-G43 helper cells. At 24hours pi, the virus particles were concentrated from the cell culture supernatant by ultracentrifugation and dissolved in SDS sample buffer. The viral proteins were separated by SDS-PAGE under non-reducing conditions and blotted onto nitrocellulose membrane. Antigens were detected with a COVID-19 convalescent serum (-S) and a rabbit polyclonal immune serum directed to the VSV G and M proteins (-G/M). The blots were derived from the same experiment and were not further processed. Inhibition of virus entry using neutralizing antibodies directed to either the VSV G protein or the SARS-CoV-2 spike protein. Vero E6 cells were inoculated in the absence or presence of the indicated neutralizing antibodies with VSV*M G-S produced on either Vero E6 or BHK-G43 cells and with VSV*G-G grown on BHK-G43 cells. Infected cells were detected 24h pi taking advantage of the GFP reporter. Bar = 100m.",yes
PMC4195902,Figure_4,oa_package/c7/e5/PMC4195902.tar.gz,"[' 4A-B shows representative perforin/GrzB flow cytometry dotplots ( 4A) and the mean distribution of intracellular perforin/GrzB subpopulations ( 4B) for memory T cells and NK cells (n = 4).', '\nComparison of GrzB and perforin expression between memory CD4, memory CD8, and natural killer cells.', 'Despite little to no intracellular perforin expression by memory CD4 T cells, perforin was secreted with GrzB by stimulated memory CD4 T cells as determined by ELISA, but in a pattern distinct from memory CD8 T cells ( 4C-D).']","Figure 4 Representative GrzB/perforin dotplots of memory CD4, memory CD8, and NK cells. Cells were purified from peripheral blood, and 510 cells (in 1 ml medium) were untreated (UT) or stimulated with IL2 (100 ng/ml) or anti-CD3 only mabs (1 g/ml) for 24 hrs +/- brefeldin. Mean (n=4) distribution of perforin/GrzB subsets of memory CD4, memory CD8, and NK cells. Extracellular GrzB and perforin production by memory CD4, memory CD8, and NK cells after 24 hrs culture +/- brefeldin (meansem, n=3-4, p<0.05 comparing UT NK cells to UT memory CD4 and memory CD8 T cells, p<0.05 comparing IL2-treated NK cells to IL2-treated memory CD4 and memory CD8 T cells, p<0.05 comparing UT or IL2-treated NK cells to UT or IL2-treated memory CD4 T cells).",yes
PMC9468748,Figure_3,oa_package/a8/e4/PMC9468748.tar.gz,[],"Figure3 Imaging and histologic findings in a 63-year-old man with primary mediastinal MTWLS (case 3). Axial, coronal and sagittal chest CT images with contrast enhancement reveal a 7.37.09.0-cm multiple cystic region and a round or oval middle anterior mediastinal mass (white arrow) adjacent to the mediastinum pleura and large blood vessels. The measurable solid components showed significant enhancement. Hematoxylin-eosin (H-E)stained section of the mass reveals spindle cells forming multiple micronodules separated by abundant interstitial lymphocytes. Cyst walls (yellow arrow) were covered with nodular tumor. Lymphocytes had diffusely strong positive immunostaining for CD20 (= B cells), and the epithelial cell nests were negative for CD20.",yes
PMC9614018,Figure_1,oa_package/13/fa/PMC9614018.tar.gz,['SP improves cardiac functions in the T2D mouse model.'],"Figure 1 SP improves cardiac functions in the T2D mouse model. Db/db mouse was selected as the research object of the T2D model, and the related indexes were determined at week 12. Blood glucose concentration, insulin resistance test, serum insulin concentration, triacylglycerol, total cholesterol, echocardiography, left ventricular ejection fraction, left ventricular fractional shortening, left ventricular internal dimension systole, and left ventricular internal dimension diastole * < 0.05 . Control group; < 0.05 . T2D group ( = 8).",yes
PMC6290765,Figure_2,oa_package/3f/33/PMC6290765.tar.gz,"[' 2a).', ' 2c).', ' 2b d).', ' 2-6 Sialylation of endometrial cells is increased by TGF- 1 treatmentIshikawa, THESCs, or 12Z cells were incubated with TGF- 1 for 48 h.', '001 compared with the control groupThere are more than 20 sialyltransferases that link sialic acid via their second carbon (C2) to the carbon atom at position C3 ( 2 3 sialyltransferase) or C6 ( 2-6 sialyltransferase) of Gal/GalNAc glycan or to C8 ( 2-8 sialyltransferase) of another sialic acid22.']","Fig. 2 2-6 Sialylation of endometrial cells is increased by TGF-1 treatment Ishikawa, THESCs, or 12Z cells were incubated with TGF-1 for 48h. The expression of 2-3 or 2-6 sialic acid epitopes in endometrial or endometriotic cells was determined by lectin blot , or lectin FACS analysis , using biotin-labeled MAL II and SNA, respectively. Data from the lectin FACS analysis are expressed as the meanSD of three independent experiments. Red asterisk indicates increased protein expression in the TGF-1-treated group compared with the control group. * <0.05 and *** <0.001 compared with the control group",yes
PMC11485213,Figure_5,oa_package/74/79/PMC11485213.tar.gz,"['Two examples in which the interobserver concordance improved with the aid of the AI solution are illustrated in .', 'FIG 5.']","FIG 5. Examples of the AI algorithm's effect on pathologists' review. Shown are slides with borderline HER2 IHC scores of 0/1+ (A) without AI and (B) with AI (total percentage of stained cells is 3.6% per the AI), both at 18 magnification (0.56 m/pixel) with higher magnification of 40 (0.25 m/pixel) insert so that the cell overlays provided by the AI solution can be visualized, and slides scored HER2 1+/2+ (C) without AI and (D) with AI (percentage of faint stained cells is 23.2% and moderate complete and incomplete 2% per the AI) at 18 (0.56 m/pixel) with higher magnification of 40 (0.25 m/pixel) insert; (B and D) red contour line marks the invasive tumor area detected by the AI, with (D) DCIS correctly excluded by the AI from the area of interest; (B) HER2 score and detected % for different cell staining patterns are provided in the AI slide report, with cell overlay (small colored circles) indicating different HER2 cell staining patterns detected by the AI (eg, empty bluenot stained, greenfaintly stained, etc). AI, artificial intelligence; DCIS, ductal carcinoma in situ; HER2, human epidermal growth factor receptor 2; IHC, immunohistochemistry.",yes
PMC3467239,Figure_1,oa_package/64/8d/PMC3467239.tar.gz,"['Incubation of all bacteria with BMDCs led to cellular activation, as shown by an increased frequency of BMDCS expressing high levels of the activation markers CD86 and CD40 (gate) as compared to medium stimulated BMDCs (A).', 'All three bacterial strains induced similar levels of TNF (B).', 'Similarly, all bacteria induced IL10 (B), but the IL10 response was highest for L.', 'salivarius-treated BMDCs, albeit not statistically significant (B).', 'g001Probiotic-induced dendritic cell activation in vitro.']",10.1371/journal.pone.0047244.g001,yes
PMC7430587,Figure_5,oa_package/09/47/PMC7430587.tar.gz,"['Histologically, all three AGMs euthanized at 5 dpi developed mild multifocal neutrophilic bronchointerstitial pneumonia (A E, O).', 'Polymerized fibrin, highlighted by IHC, colocalized with acute inflammation within the bronchial lumen, alveolar spaces, alveolar walls and ulcerated regions of the trachea and bronchus (C).', 'Trichrome stain of representative lung sections identified modest collagen deposition within multifocal regions of alveolar septae (D).', 'Positive IHC labeling was noted diffusely within the cytoplasm of respiratory epithelium of the bronchus (O) and less in type I and type II pneumocytes (E).', 'Histologic features include expansion of alveolar septae with macrophages, lymphocytes, and very rarely neutrophils (F, G).', 'Wispy, pale eosinophilic, acellular material also multifocally expanded the alveolar walls and stained as immature collagen with trichrome staining (I).', 'Polymerized fibrin was present within medium and small caliber vessels but was not associated with an obvious adherent thrombus (H).', 'No immunolabeling for SARS-CoV-2 was noted with IHC in any of the examined tissue sections from this 34 dpi cohort (J).', 'Comparative pulmonary histologic lesions in AGMs infected with SARS-CoV-2.']","Figure 5 Comparative pulmonary histologic lesions in AGMs infected with SARS-CoV-2. Representative tissues of AGM from 5 dpi (A-E & O) and 34 dpi (F-J). SARS-CoV-2 nave tissues from an AGM (K-N). H&E staining at low magnification (20x) (A, F, & K) and higher magnification (40x) (B, G, L, and F inset) of pulmonary alveolar septae and alveolar spaces. Moderate neutrophilic bronchiolitis and alveolitis and mild interstitial pneumonia with congestion (A & B) Moderate lymphohistocytic interstitial pneumonia with congestion, mild alveolar wall fibrosis (F & G) and moderate perivascular lymphocytic cuffs (F inset). No significant lesions (K & L). IHC for anti-fibrin antigen (red) (C, H & M). Alveolar spaces are partially to completely flooded with fibrin (C) Minimal intravascular fibrin labeling (H) and no significant fibrin immunolabeling (M). Trichrome special stain for collagen (blue) (D, I & N). Minimal to mild alveolar wall collagen deposition (D) moderate alveolar wall collagen deposition (I) and minimal collagen staining of alveolar wall basement membranes (N). IHC labeling for anti-SARS-CoV2 antigen (red) (E, J & O). IHC positive type I pneumoncytes (black arrows) and type II pneumoncytes (white arrow) localized with alveolar inflammation (E), No immonolabeling (J) and IHC positive labeling of respiratory epithelium of the bronchus (O). Images captured at 20x (M, D, I, N, & J) and 40x (C, H, E, & O).",yes
PMC6413539,Figure_3,oa_package/9d/3a/PMC6413539.tar.gz,"['The TOF measurement volume covered the hippocampal area, which allowed us to achieve very high spatial resolution while keeping the acquisition time as short as possible ().', 'A) 7 T T1 sequence (grayscale background), 7 T T2 sequence (red), 7 T TOF sequence (blue), hippocampus (yellow).', '2.', 'An overview of the basic visualization during the analysis task is provided in D, where the hippocampus was visualized in addition to the vessels of interest; the PCA was the most prominent with its branches penetrating the hippocampus.']","Fig. 3 A) 7TT1 sequence (grayscale background), 7T T2 sequence (red), 7T TOF sequence (blue), hippocampus (yellow). B) 7T TOF MRA. C) 7T TOF on 7T MPRAGE. D) Mevislab reconstruction of 7T TOF MRA (with the hippocampal mask).",yes
PMC3554372,Figure_5,oa_package/e4/1e/PMC3554372.tar.gz,"['Staining of hippocampal sagittal sections clearly showed a robust increase in phospho- immunoreactivity at the AT8 epitope in NODH mice and to a much lesser extent in the NODG group in comparison with ICR controls (A C).', 'However, we did not detect significant changes in total or phospho- cellular localization, as revealed by AT8, total , and DAPI staining patterns (D I).', 'Amyloid precursor protein metabolism in NOD mice.']","FIG. 5. Regional anatomical localization of phosphorylated protein in adult NOD mice. Unmixed fluorescent photomicrographs of hippocampal sagittal sections are shown with AT8 (Red, ), Total Tau (Green, ), or merged with DAPI ( ), for the following conditions: control (ICR mice, ), glycosuric NODG mice ( ), or glycosuric and hypothermic NODH mice ( ). All images were taken at original magnification 5.",yes
PMC5718516,Figure_7,oa_package/fe/bb/PMC5718516.tar.gz,"['g006""/>Inhibitor LY294002 and RSV block the Cav-1 expression in HEK293 cells cultured in DMEM with high glucoseAs shown in , HG up-regulated the expression of Cav-1 mRNA and protein levels.', 'g007Inhibitor LY294002 and RSV block the Cav-1 expression in HEK293 cells cultured in DMEM with high glucose.']",10.1371/journal.pone.0189156.g007,yes
PMC3348685,Figure_1,oa_package/08/5f/PMC3348685.tar.gz,"['Despite postoperative radiotherapy and chemotherapy, the patient who was operated on for breast cancer metastasis (40/F) died during postoperative month 16; patients who were operated on for lung cancer metastasis (65/F) () and bladder cancer metastasis (55/F) died during postoperative month 12; the patient who was operated on for renal cancer metastasis (57/M) died in postoperative year 3.', '\nA 65-year-old female patient with lung cancer metastasis in her L5 vertebra (patient #3).']",Figure 1 A 65-year-old female patient with lung cancer metastasis in her L5 vertebra (patient #3). A) Preoperative sagittal T2-weighted MR imaging of the patient; B) Preoperative axial T2-weighted MR imaging of the patient; C) Lateral radiographic view after the operation; D) Antero-posterior radiographic view after the operation.,yes
PMC7367569,Figure_1,oa_package/c3/58/PMC7367569.tar.gz,['[56]Treatment response to WHO MDT.'],Figure 1 Treatment response to WHO MDT. (a and b) Lepromatous patient before treatment and after 1 year of MDT-MB. (c and d) Tuberculoid patient before treatment and after 6 months of MDT-PB,yes
PMC7817172,Figure_4,oa_package/e8/67/PMC7817172.tar.gz,"['There was necrosis of the epithelium with presence of necrotic materials and mononuclear inflammatory cells in the gastric lumen (A).', 'The IP assessment revealed strong positive stain that concentrated especially on the epithelial cells of the stomach (B).', 'Fascinatingly, foetuses of group 4 developed severe and generalised necrotic gastritis leading to severe haemorrhages into the lumen with moderate congestion and intense mononuclear inflammatory reaction (C).', 'The IP staining revealed intense brown staining of the entire stomach tissue (D).', '.']","Figure 4. Histology section of foetal stomach of group 3 (A, B) and 4 (C, D). (A) Section of foetal stomach of group 3 that was infected for 30days showing moderate necrosis and inflammation (long arrows). Note the necrotic remnant in the lumen (short arrows). HE x200. (B) Mild positive immuno-peroxidase staining mostly at the epithelial cells of the stomach (arrows). IP x200. (C) Foetal stomach section of group 4 that was infected for 60days showing extensive necrosis and inflammation of the stomach. HE x200. (D) Intense and widely distributed immuno-peroxidase staining in the entire stomach section. IP x200. (E) Normal histological feature of the stomach of a foetus of non-infected group 1. HE x200. (F) Negative immune-peroxidase staining of the stomach of a foetus of non-infected group 1. IP x200.",yes
PMC8688964,Figure_3,oa_package/99/e7/PMC8688964.tar.gz,"['3Loop snare being deployed distal to free end of PICC, with distal end of PICC being grasped by loop snare and being pulled into vascular access sheath En bloc retrieved PICC line grasped by loop snare The procedure was performed with continuous cardiac monitoring and in presence of an intensivist.']","Fig. 3 Loop snare being deployed distal to free end of PICC, with distal end of PICC being grasped by loop snare and being pulled into vascular access sheath",yes
PMC8407264,Figure_4,oa_package/1f/99/PMC8407264.tar.gz,"['One of the main advantages of HFUS is the visualization with an option of direct internal skin structure measurements (), and the detection of micro-morphological changes in different skin pathologies, including skin neoplasms ().', '75 MHz HFUS image of normal skin taken from the inner forearm.']","Fig. 4 75 MHz HFUS image of normal skin taken from the inner forearm. e epidermis, pd papillary dermis, rd reticular dermis, bv blood vessels, fat subcutaneous fat tissue, fas fascia, msc muscle, hf hair follicle, map musculus arrector pili",yes
PMC10917200,Figure_1,oa_package/5f/1f/PMC10917200.tar.gz,"[' 1A).', ' 1B).', ':Samples of behavioral tests.', 'MRI data acquisitionA 3Telsa MRI system (EXCITE, General Electric, Milwaukee, Wisc.', ' 1 and Table 1), which echoed previous findings.']",Figure 1: Samples of behavioral tests. (A) stimulus used in Cambridge Face Memory Test; (B) software interface for the Radiological Expertise Task.,yes
PMC5534204,Figure_6,oa_package/e9/a3/PMC5534204.tar.gz,"[' 6a shows that concentration-dependent bradykinin-induced dilations were significantly decreased in MetS pig coronary arteries pre-contracted with 100 M histamine (n = 10) as compared to those from Lean pigs (n = 7).', ' 6a).', ' 6b shows that similar results were obtained in 30 mM KCl pre-contracted coronary artery rings (MetS, n = 5; Lean, n = 8, and MetS, n = 5).', '', 'The two-way ANOVA test followed by the Student Newman Keuls post hoc test\nPattern of TRPC immunostaining in the coronary artery wallTo determine whether the MetS-associated increases in TRPC1 and TRPC6 protein expression occur locally or globally within the coronary wall immunohistochemical studies were performed.']","Fig.6 Assessment of endothelial function in coronary arteries from the three tested groups using isometric tension measurements. , Concentrationresponse curves for bradykinin-induced dilation in histamine- ( ) and 30mM KCl- ( ) pre-contract coronary artery rings from Lean, MetS and MetS-SN pigs. Sample traces of bradykinin-induced coronary ring dilations are shown in . * <0.05, ** <0.01, *** <0.001, *MetS vs. Lean; <0.05, <0.01, <0.001, MetS vs. MetS-SN. The two-way ANOVA test followed by the StudentNewmanKeuls post hoc test",yes
PMC8523912,Figure_1,oa_package/c4/c6/PMC8523912.tar.gz,"['Using this algorithm, the hippocampus was accurately segmented into the following subfields: subiculum, presubiculum, parasubiculum, molecular layer, cornu ammonis (CA)1, CA2/3, CA4, hippocampal tail, hippocampal fissure, fimbria, hippocampus-amygdala transition area (HATA) and molecular and granule cell layer of the DG (GC-ML-DG) ().', 'Atlas-based segmentation of the hippocampus.']",Fig. 1 Atlas-based segmentation of the hippocampus.,yes
PMC5094992,Figure_7,oa_package/b7/c8/PMC5094992.tar.gz,['Exogenous overexpression of AIM2 suppressed xenotransplant tumor growth in nude miceBEL7402 cells and SMMC7721 cells (1 107) were subcutaneously injected into the left upper flank of the nude mice.'],"Figure 7 Exogenous overexpression of AIM2 suppressed xenotransplant tumor growth in nude mice BEL7402 cells and SMMC7721 cells (1 10 ) were subcutaneously injected into the left upper flank of the nude mice. When visible tumor appeared, tumors were injected with 30 ug of AIM2 plasmid or empty vector. ( ) The photo of the excised tumors from sacrificed nude mice of AIM2 plasmid transfected group and mock control group. ( ) qRT-PCR was performed to detect and compare the AIM2 mRNA in the AIM2 overexpressed group and mock group. *** < 0.001. ( ) Statistical analysis of the tumor volume (C) and tumor weight (D) was performed between the AIM2 transfected group and mock group. ( ) Western blot was performed to detect the activation of mTOR-S6K1 pathway in the AIM2 transfected group and mock group (E). Band intensities were analyzed for the presented bands and statistical differences were shown among indicated groups (F). * < 0.05, ** < 0.01, and *** < 0.001 for statistical differences in the indicated groups.",yes
PMC7522591,Figure_1,oa_package/53/99/PMC7522591.tar.gz,"['PET SCAN positron emission tomography: Shows multiple metastatic hypermetabolic lymphadenopathies at the level of the spleen, pancreas, right intercostal space adjacent to the xiphoid appendix, skin, and subcutaneous cell tissue adjacent to the right deltoid and in the mammary regions.', 'Chest radiograph: There are no signs of nodules, masses, consolidation, cavitation in the lung parenchyma or signs of pleural effusion.']","Fig. 1 PET SCAN positron emission tomography: Shows multiple metastatic hypermetabolic lymphadenopathies at the level of the spleen, pancreas, right intercostal space adjacent to the xiphoid appendix, skin, and subcutaneous cell tissue adjacent to the right deltoid and in the mammary regions. Hypermetabolic compromise in the soft tissues, adjacent to the right hip and another in the supraclavicular region.",yes
PMC7379707,Figure_5,oa_package/03/35/PMC7379707.tar.gz,['Primed factor XII (FXII) accelerates contact system activation in plasma.'],"Figure 5 Primed factor XII (FXII) accelerates contact system activation in plasma. Factor XII was pretreated with neutrophil elastase or vehicle, and afterwards reconstituted in FXIIdepleted plasma. FXIIaC1INH (A) and plasma kallikrein (PKa)CINH (B) complexes were measured after 15 min in the presence or absence of 11.3 nmol L PKa. The prognosed cooperative activity of both enzymes is indicated by the striped bars. (C) Highmolecularweight kininogen (HK) consumption in plasma was determined by western blotting after 15 min exposure to a concentration range of PKa. (D) Densitometric determination of cleaved HK (cHK)/HK ratio after 15 min exposure to 11.3 nmol L PKa. The prognosed cooperative activity of both enzymes is indicated by the striped bar. Data represent means SD of three separate experiments, all performed in duplicate. Data were analyzed by oneway (** < 0.005, **** < 0.0001).",yes
PMC6559703,Figure_5,oa_package/2a/32/PMC6559703.tar.gz,"['This phagocytosis enhancing effect of the SRF was higher than that caused by the known enhancer of the phagocytosis process (triamcinolone, TC) and showed no additive or cumulative effect upon TC+SRF treatment ().', 'g005Phagocytosis study of macrophages (M ) engulfing dying ARPE-19 cells upon treatment with conditional medium containing 50% subretinal fluid, as well as triamcinolone (TC).']",10.1371/journal.pone.0217548.g005,yes
PMC10504474,Figure_1,oa_package/87/46/PMC10504474.tar.gz,"['A pelvic MRI () was suggestive of penile skin inversion vaginoplasty without inguinal or pelvic lymphadenopathy.', 'Preoperative sagittal MRI of pelvis showing the bladder, the prostate and the rectum, with hyperintense lesion (circled) on anterior wall of neo-vagina.', 'The patient was taken to the operating room for wide local excision of her verrucous lesion (']","Fig. 1 Preoperative sagittal MRI of pelvis showing the bladder, the prostate and the rectum, with hyperintense lesion (circled) on anterior wall of neo-vagina. Imaging suggests penile skin inversion vaginoplasty.",yes
PMC5491844,Figure_6,oa_package/14/e2/PMC5491844.tar.gz,"['Effect of prostaglandin (PG) and endothelium-derived hyperpolarization (EDH) inhibition or superoxide scavenging on responses to methylcholine (MCh) in mesenteric arteries from advanced maternal age postpartum rats at 3 months postpartum, or equivalent age in never pregnant (virgin) controls (~13.']","Figure 6 Effect of prostaglandin (PG) and endothelium-derived hyperpolarization (EDH) inhibition or superoxide scavenging on responses to methylcholine (MCh) in arteries from advanced maternal age postpartum rats at 3 months postpartum, or equivalent age in never pregnant (virgin) controls (~13.5 months old). Relaxation of mesenteric arteries from virgin rats to MCh was reduced by Apamin and TRAM-34 (Apa, TRAM; EDH inhibitors) but was unaffected by indomethacin (indo; PG inhibitor). In postpartum rats, inhibition of MCh-induced relaxation by Apa, TRAM was reduced compared to the virgin control incubation while indo significantly enhanced MCh-induced relaxation. The effect of parity and Apa, TRAM on vasodilation to MCh (pEC ) were analyzed by two-way ANOVA: drug effect < 0.0001, with Sidak's post-test, < 0.01, = 56. Concurrent incubation with pegSOD (superoxide scavenger) did not affect MCh-induced vasodilation in either virgin or postpartum rats; however, the variability of MCh-induced relaxation was increased, < 0.001 by Bartlett's test, in the presence of pegSOD in mesenteric arteries from postpartum rats. The effect of parity and oxLDL on vasodilation to MCh (pEC ) were analyzed by two-way ANOVA with Sidak's post-test, = 5-6.",yes
PMC9553250,Figure_4,oa_package/31/9a/PMC9553250.tar.gz,[],"FIGURE 2 (AC) Mitochondrial respiration in aorta (Aac) and carotid (Bac), lipid metabolism, and aorta protein expression of mitochondrial complexes (Cae). Permeabilized vessels were exposed to substrates and inhibitors mimicking lipid metabolism and background oxygen consumption or leak state (state 2), oxidative phosphorylation (+ADP, state 3), maximum oxidative phosphorylation [succinate, state 3S (A and Ba)], state 4 [+oligomycin (A and Bb), and uncoupled respiration [+FCCP (A and Bc)] were determined. Respiration rates were normalized to tissue dry weight ( =78). Effect of temperature less than 0.05, less than 0.08, two-way ANOVA. For (Cae), aorta tissue was processed for protein analysis via western blot analysis ( =8). Blots were probed for mitochondrial complexes IIV using a single antibody containing subunits of all complexes. =0.08 interaction (long bar) and temperature (elongated bracket) effect, two-way ANOVA; (Cae) data are meanSEM. ANOVA, analysis of variance.",yes
PMC10589586,Figure_3,oa_package/c8/45/PMC10589586.tar.gz,['.'],"Figure 3. Collage of microscopic images of the most relevant cases, in usual Hematoxylin-Eosin staining, objective x100. (A) Nonspecific chronic sialadenitis. (B) Pleomorphic adenoma. (C) Warthin tumor. (D) Polymorphous adenocarcinoma. (E) Acinic cell carcinoma. (F) Salivary duct carcinoma. (G) Non-Hodgkin lymphoma. (H). Squamous cell carcinoma metastasis. (I) Malignant melanoma metastasis.",yes
PMC10491916,Figure_5,oa_package/67/2c/PMC10491916.tar.gz,"['44 These data suggest that Nrg4 may induce autophagy through the AMPK and mTOR signaling pathway, which in turn attenuates hepatic steatosis in aged mice (', ' 5The potential roles of Nrg4 in NAFLD, cancer, and thyroid disorders.', ' 5In vivo, Nrg4 was able to suppress the expression of lipogenic genes (Srebf1, Acaca, Fasn, and Scd1) in the liver (', 'A study has shown that transgenic expression of Nrg4 inhibits the expression of PPAR and its target genes (Cd36, Mgat1, and Fabp4) in the liver (', '5"">CancerNrg4 is involved in the occurrence and development of many cancers (']","Figure5 The potential roles of Nrg4 in NAFLD, cancer, and thyroid disorders. Nrg4 has a protective role against non-alcoholic fatty liver disease (NAFLD). In hepatocytes, Nrg4 may induce autophagy through the activation of AMPK signaling and the inhibition of mTOR signaling. This in turn attenuates hepatic steatosis in aged mice. Nrg4/ErbB4 signaling can increase phosphorylation of endogenous ErbB3 and STAT5 proteins, and activated STAT5 inhibits LXR transcriptional activity, which inhibits the expression of , the gene encoding Srebp-1c that promotes lipogenesis. Moreover, Nrg4 inhibits the expression of PPAR and its target genes ( , , and ) in the liver, thus preventing hepatic steatosis. Nrg4 is able to suppress the expression of lipogenic genes ( , , , and ) in the liver. Whether Nrg4 could ameliorate NAFLD by promoting fatty acid -oxidation needs further investigation. Furthermore, Nrg4 can inhibit the JNK1/2 phosphorylation by activating AKT and then reduces the ubiquitination and proteosomal degradation of FADD-like apoptosis regulator (c-FLIP ) to exert cytoprotective effects, which hinders the development of hepatic steatosis to NASH. Nrg4 is shown to play potential roles in cancer, thyroid disorders, diabetic nephropathy, and polycystic ovary syndrome. In cancer, Nrg4 is highly expressed in melanoma, prostate cancer, and malignant lymphoma in the gastrointestinal tract, and it can promote the proliferation of the above cancer cells. However, Nrg4 is down-regulated in a mouse model of NASH-related hepatocellular carcinoma (HCC). Nrg4 can inhibit NASH-related HCC by impairing tumor-prone liver immune microenvironment. In addition, Nrg4 and its receptor ErbB4 were significantly reduced in cancerous tissues from patients with bladder cancer and gastric cancer. The exact roles of Nrg4 in different cancers need further exploration. In thyroid disorders, Nrg4 levels were positively correlated with serum-free T3, free T4, thyroid peroxidase antibody (TPOAb), and thyroglobulin antibody (TGAb) levels, but negatively correlated with thyroid stimulating hormones (TSH). In diabetic nephropathy, Nrg4 can attenuate renal function injury, tubulointerstitial fibrosis, inflammation and suppress the expression levels of advanced glycosylation end products (AGEs). In polycystic ovary syndrome, serum Nrg4 levels are significantly elevated in patients with polycystic ovary syndrome, and the role and mechanism of Nrg4 in polycystic ovary syndrome need to be further explored.",yes
PMC11577142,Figure_3,oa_package/28/83/PMC11577142.tar.gz,['Coronal view CT depicting the appendix fistulized to the iliopsoas accompanied by abscess formation.'],Figure 3 Coronal view CT depicting the appendix fistulized to the iliopsoas accompanied by abscess formation. Whitedotted line: border of the appendix. Red line: border of the iliopsoas.,yes
PMC7930738,Figure_1,oa_package/ca/15/PMC7930738.tar.gz,"['These GPCRs comprise protease-activated receptor-2 (PAR-2), neurokinin-1 receptor (NK1R), histamine receptors H1 and H4 (H1R/H4R) and mas-related G-protein coupled receptors (MRGPRs) as well as tropomyosin receptor kinase A (TrkA) receptor (, Table 1).', 'Involvement of different receptors/channels in neuro-immune interactions in pruritus.', 'Potential mediator and promising receptor therapeutic targets in the skin as well as in peripheral nerves comprises TRPV1, TRPA1, IL-31RA, TSLPR, PAR-2, NK1R, H1R and H4R, MRGPRs and TrkA, which are highlighted in this review (, Tables 1, 2).']","Figure 1 Involvement of different receptors/channels in neuro-immune interactions in pruritus. There is a complex interplay between neurons and immune cells in transmission of pruritus and inflammation. Several receptors act as a bridge between the neuronal and immune network and function as pruritus mediators. These receptors are located on neurons, but also expressed by different non-neuronal cells (e.g., basophils, dendritic cells, eosinophils, keratinocytes, mast cells, macrophages and monocytes, neutrophils or T cells): Transient receptor potential vanilloid 1 (TRPV1) and ankyrin 1 (TRPA1), IL-31 receptor A (IL-31RA) and the oncostatin-M receptor (OSMR), thymic stromal lymphopoietin receptor (TSLPR), protease-activated receptor 2 (PAR-2), neurokinin-1 receptor (NK1R), histamine receptors H1/H4 (H1R/H4R), mas-related G-protein coupled receptor X2 (MRGPRX2), tropomyosin receptor kinase A (TrkA).",yes
PMC4229875,Figure_11,oa_package/d2/14/PMC4229875.tar.gz,[],"Figure 11 Longitudinal sections of microvessels from animals that were exposed to either one or three 74.5 kPa blasts and were sacrificed 24 hours later. Strictures where there is narrowing of the vascular lumen are indicated by arrows. The dendrite (D) of a nearby neuron is indicated in panel . Panel shows a region exhibiting a microvascular stricture (box in ). A higher power image shows that the lumen of this microvessel has been occluded by amorphous material and opposing endothelial cell walls appear to have fused . Panel shows complete luminal occlusion by amorphous material. The boxed region in panel is illustrated at higher power in panel . Note that despite the destruction of the microvessel architecture at the site of the strictures, the surrounding neuropil appears normal. Panels and illustrate longitudinally cut microvessels from non-blast exposed control brains. Scale bar 1 m - ; 1.2 m ; 0.2 m ; 2.5 m ; 0.5 m ; 3.5 m - .",yes
PMC7577845,Figure_10,oa_package/1c/ce/PMC7577845.tar.gz,[],Fig. 10 Multisystem inflammatory syndrome in children (MIS-C). Superficial and deep scant perivascular inflammation (arrows) with microthrombi in the papillary dermal capillaries (arrowheads) (features of thrombotic microangiopathy). The perivascular inflammatory infiltrate (arrows) consists mostly of lymphocytes and some histiocytes (hematoxylin and eosin; original magnification 100; 400) (courtesy of Drs Michael Occidental and George Jour),yes
PMC11482725,Figure_3,oa_package/3b/21/PMC11482725.tar.gz,"['Trimetazidine (TMZ), also indicated for angina, targets 3-Keto Acyl Thiolase (Acaa), a key enzyme in fatty acid -oxidation, facilitating a metabolic shift towards glycolysis over FAO for energy needs (A).', 'g003Regulation of bacterial growth in neutrophils by mitochondrial fatty acid metabolism.', 'Inhibition of fatty acid metabolism using ETO, Ran, Mil, or TMZ led to a dose-dependent decrease in both MFI and bacterial CFU at 24 hpi (B 3E).', 'For this purpose, octanoic acid, a medium-chain fatty acid, was administered alongside ETO treatment (F).', 'In contrast, treatment of neutrophils with UK5099 or R162 did not affect the bacterial load within these cells, suggesting that the observed effects on bacterial survival were specifically due to the disruption of fatty acid oxidation as a critical energy source (F).', 'Analysis of Mtb infection in neutrophils isolated from these genetically modified animals demonstrated a significant reduction in both the MFI of smyc ::mCherry and the CFU counts, in comparison to their littermate controls (G).', '(F) Flow cytometry analysis of neutrophil cell death post treatment with mitochondrial metabolism inhibitors (Etomoxir, Oxfenicine, UK5099, R162, as used in F) or with Etomoxir after supplementation with the medium-chain fatty acid, Octanoic acid, at 24 hours pi (% FVD- cells).', 'Regarding the mouse model that I mentioned above, in G, they only showed the genotyping results.', '25 x 10^6 Mtb/well obtained from mock-treated cells in B.', 'Is there a reason why B data is shown in a different graph format than Figs.', '- F: Octanoic acid alone improves Mtb growth, which makes it somewhat difficult to draw conclusions regarding the phenotype observed with ETO + octanoic acid.']",10.1371/journal.ppat.1012188.g003,yes
PMC7573241,Figure_3,oa_package/8e/63/PMC7573241.tar.gz,"['The bacillary layer measured spanning from the outer nuclear layer until the outer segment of the photoreceptors (A).', '005; B).', 'Going into detail, by assessing only the bacillary layer thickness at 4 days (C), a significant reduction was detected in controls compared to native samples (p = 0.']","FIGURE 3 Total and bacillary layer thickness measurement in stained retinas. Representative images of H&E-stained retinas for all three explantation methods and the native group in 400 (upper panel) and 630 magnification (lower panel). At 4 days, a significant reduction of the total retinal thickness in control compared to native samples ( = 0.005) was observed, while a preservation was detected in the novel techniques filter and tweezers when compared to native samples. Comparing tweezers to control samples, a significantly thicker retinal thickness was noted ( = 0.009), while a similar thickness was observed between filter and control retinas. Similar results were found at 8 days. A reduction of the total retinal thickness was revealed comparing control to native samples ( = 0.04). A conservation of the total retinal thickness was detected comparing filter and tweezers to native or control retinas. A significant thinning of the bacillary layer was seen in the control group compared to native ( = 0.04) and filter samples ( = 0.046). However, a better-preserved bacillary layer was noted in the tweezers method compared to the native and control samples at 4 days. A reduction in the bacillary layer thickness was measured at day 8 comparing the control and native samples ( = 0.001). The novel methods filter and tweezers maintained the bacillary layer thickness better than did control retinas. OS, photoreceptor outer segments; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer. Scale bars: 20 m, values are mean SEM. = 910/group. * < 0.05 and ** < 0.01 vs. native group; < 0.05 and < 0.01 vs. controls.",yes
PMC8524197,Figure_2,oa_package/a4/c8/PMC8524197.tar.gz,"['The immunostaining with the most frequently used A plaque-specific antibodies, 6E10 and 4G8, revealed multiple plaque subtypes in A O-monkeys as in patients with AD, such as compact plaques mostly having clear-cut outlines (s 2A 2D, left panels), cotton-wool plaques ( 2A, right panel), cored plaques with miliary focus ( 2C, right panel), as well as diffuse plaques usually displaying ill-defined surfaces and flake-like deposits (s 2B and 2D, right panels), indicating that A plaques in the cortex of A O-monkeys morphologically and immunoreactively resembled those in patients with AD.', 'In addition, the A plaques in A O-monkey brain were recognizable by other standard measurements that are usually performed to detect A plaques in the patient brain, including silver ( 2E), Thioflavin S ( 2F), and Congo Red staining ( 2G).', 'Aligned with the immunostaining results, the western blot with 6E10 detected A -positive bands in FC and TC of A O-monkeys, which was similar to the band from 5XFAD mouse brain with A plaques ( 2H).', 'There was no detectable A -band from brain tissues of control monkeys ( 2H), which was consistent with the previous findings (Oikawa et al.', ' 2The characterization of developed A plaques in A O-monkeys(A and B) The representative subtypes of A plaques detected by immunohistochemical (A) and immunofluorescent (B) analysis with 6E10.']","Figure1 The development of A plaques in AO-monkeys (A) Schematic diagram of delivering AOs into the parenchyma of cynomolgus monkeys (n= 7). (B) Western blot analysis of synthetic AOs with the monoclonal antibody 6E10 immediately prior to each injection. (C) Dot blot analysis of synthetic AOs with A oligomeric and fibrillary antibodies 6E10, 4G8, and OC. (DG) Immunohistochemical analysis with 6E10 for detecting A plaques in brain sections from anterior to posterior cerebrum of AO-monkeys and representative whole brain sections containing striatum and prefrontal cortex (D); frontal cortex, striatum, and temporal cortex (E); parietal cortex, hippocampus, and entorhinal cortex (F); and parietal cortex, hippocampus, and temporal cortex (G). In the right panel, enlarged views of the outlined regions clearly indicate the massive A plaques in different brain regions. (H) Immunohistochemical analysis with 6E10 for detecting A plaques in representative whole brain section from cerebrum of the control monkey. In the bottom panel, enlarged views of the outlined regions indicate that A plaques are absent or very sparse in different brain regions. (I) Quantification analysis of the numbers of A plaques in the cerebrum of control (n= 7) and AO- (n= 7) cynomolgus monkeys (p= 0.0345). (J) Quantification analysis of the burden of A plaques in the cerebrum of control (n= 7) and AO- (n= 7) monkeys (p= 0.0394). Data are represented as mean SEM. Each symbol represents an individual cynomolgus monkey. Statistical differences are evaluated with two-tailed unpaired Student's t test. p< 0.05. Scale bars: 5mm (whole brain images in CG), 200m (enlarged views in CG). Abbreviations: PFC, prefrontal cortex; FC, frontal cortex; TC, temporal cortex; PC, parietal cortex; HPC, hippocampus; and EC, entorhinal cortex. See also and .",yes
PMC5436503,Figure_5,oa_package/5a/12/PMC5436503.tar.gz,[' [18F]GE-180 ex vivo autoradiography of 8.'],"Figure 5 (A) Representative autoradiography images and Nissl staining of the same brain sections show a similar pattern of uptake to that observed in [ F]GE-180 PET/MR images. White and red arrows point to cortex and hippocampus respectively. (B) Mean signal intensity for specific brain regions normalized to rostral thalamus (rThal) for all four groups of mice ( = 7 per group). *p-value <0.05, **p-value <0.01, ***p-value <0.005.",yes
PMC11095255,Figure_6,oa_package/65/60/PMC11095255.tar.gz,"['The scan showed development of venous collaterals, which drain the distal splenic vein and inferior mesenteric vein through the lesser omentum ().', 'CT scan at 3 years, with development of venous collaterals that drain distal splenic vein and inferior mesenteric vein through the lesser omentum.']","Figure 6 CT scan at 3 years, with development of venous collaterals that drain distal splenic vein and inferior mesenteric vein through the lesser omentum. PV portal vein, SMV superior mesenteric vein, SV splenic vein, IMV inferior mesenteric vein, CV collateral veins.",yes
PMC4334633,Figure_5,oa_package/93/1f/PMC4334633.tar.gz,"[""Cyst's view after removal shows cysts inner wall and internal area.""]",Fig. 5 Cyst's view after removal shows cysts inner wall and internal area.,yes
PMC7988374,Figure_2,oa_package/13/f8/PMC7988374.tar.gz,"[' 2a, b) and a lung carcinoid (', 'Histopathology is consistent with an ovarian carcinomaAxial non-contrast CT shows a 9-mm nodule in the right lower lobe.']",Fig. 2 Axial non-contrast CT shows a large soft tissue mass in the right pelvis. Subsequent pelvic ultrasound confirms a mixed solid/cystic mass arising from the right ovary. Histopathology is consistent with an ovarian carcinoma,yes
PMC8578039,Figure_6,oa_package/4e/ac/PMC8578039.tar.gz,['3DiscussionSLD most of the time is a unilateral injury because the probability of sustaining an identical complex ligament injury to both wrists would be extremely small [5].'],Fig. 6 Right wrist X-ray after K-wire removal.,yes
PMC4042636,Figure_1,oa_package/79/a4/PMC4042636.tar.gz,"['Neither strain exhibited an inflammatory phenotype in the absence of LACK sensitization ( A).', ', PBS)-sensitized CX3CR1+/+ mice, LACK-sensitized CX3CR1+/+ mice exhibited a significant skin inflammatory response, characterized by a 50% increase in epidermal thickening ( B) associated with more pronounced hyperkeratosis, spongiosis, and dermal cellular infiltrates, including mast cells, eosinophils, MHC-II+, and CD4+ T cells (', 'In sharp contrast, CX3CR1gfp/gfp mice did not develop a skin inflammatory response upon LACK sensitization (, A and B).', 'Compared with PBS-sensitized CX3CR1gfp/gfp mice, only MHC-II+ cell numbers were increased, but to a lesser extent than in LACK-sensitized CX3CR1+/+ animals ( C).', 'Humoral response was also altered in CX3CR1gfp/gfp compared with CX3CR1+/+ mice, with decreased total Th2-associated IgE concentrations (but not IgG1 titers), as well as decreased Th1-associated antigen-specific IgG2a titers ( D).', '.', 'As in the human pathology, epicutaneous sensitization also induced lung inflammation and airway hyperresponsiveness (AHR) after a single antigenic airway challenge.', 'Airway resistance upon LACK sensitization was significantly lower in CX3CR1gfp/gfp compared with CX3CR1+/+ animals ( E).', 'Furthermore, cellular inflammatory infiltrates in the bronchoalveolar lavage fluid (BALF) of LACK-sensitized CX3CR1gfp/gfp mice were decreased by 32% for macrophages, 70% for lymphocytes and eosinophils, and 40% for neutrophils compared with BALF from CX3CR1+/+ mice ( F).', '(C) AD was induced in WT mice as described in the legend of , and equal numbers of LACK-specific CX3CR1gfp/gfp and CX3CR1+/gfp Th1 (top) or Th2 (bottom) cells were coinjected at day 41.', '(E G) AD was induced as described in the legend of .']","Figure 1. AD was induced on abdominal skin in CX CR1 and CX CR1 mice by epicutaneous LACK sensitization for three 1-wk periods, with a 2-wk interval between applications. At day 49, sera were collected and animals were challenged by LACK nebulization. At day 50, AHR to increasing concentrations of methacholine was measured by invasive plethysmography. Then, mice were sacrificed and BALF was collected and analyzed on cytospin preparations. Skin samples were collected at the site of sensitization. (A) May-Grnwald Giemsa staining of skin sections. Black arrows indicate mast cells and, in the inset, eosinophils. (B) Epidermal thickness. (C) Eosinophil, mast cell, MHC II , and CD4 T cell numbers in dermis. (D) Ig concentrations in serum. Total IgE (left), LACK-specific IgG (middle), and LACK-specific IgG (right) concentrations are shown. Horizontal bars indicate mean. (E) AHR to increasing methacholine concentrations. Resistance was evaluated by invasive plethysmography. (F) Lung inflammatory response: total number of cells, macrophages, neutrophils, lymphocytes, and eosinophils in BALF. Data are expressed as mean SEM ( = 610 animals per group). One out of two independent experiments is shown for each panel. *, P < 0.05; ** P < 0.01.",yes
PMC7334550,Figure_2,oa_package/f1/d9/PMC7334550.tar.gz,"['The tip of a radiofrequency (RF) needle is positioned under both intravascular ultrasound (IVUS) guidance and biplane fluoroscopic guidance A: IVUS imaging confirms adequate tenting position with the RF needle B: Illustration of tenting position with the RF needle C: Angiogram shows dilatation of the created re-entry tear using a balloon catheter D.', 'Symptoms of leg ischemia disappeared dramatically after the procedure.']",Fig. 2 The tip of a radiofrequency (RF) needle is positioned under both intravascular ultrasound (IVUS) guidance and biplane fluoroscopic guidance A: IVUS imaging confirms adequate tenting position with the RF needle B: Illustration of tenting position with the RF needle C: Angiogram shows dilatation of the created re-entry tear using a balloon catheter D.,yes
PMC11351501,Figure_2,oa_package/2a/45/PMC11351501.tar.gz,"['The diagnosis of primary membranous nephropathy was established after analyzing the pathology specimen through basic stains, immunohistochemistry, and electron microscopy (figure 2).', '\nRenal biopsy.']","Figure 2 Renal biopsy. Twenty glomeruli, one sclerosed, are observed. The glomeruli show marked podocyte activation associated with diffuse thickening of the glomerular basement membranes in the hematoxylin-eosin staining (40X). Evidence of spikes and holes in the methenamine silver stain (40X). The interstitium shows few areas of interstitial fibrosis and tubular atrophy. Direct immunofluorescence stains: Intense granular staining is observed in the basement membranes with IgG (4+), C3 (2+), kappa (4+), lambda (4+) (40X), and anti-PLA2R (2-3+). 2C. IgG subclasses show a predominance of IgG4 (4+) over IgG3 (3+), IgG2 (1+), and IgG1 (2+). Electron microscopy showing electron-dense deposits of mesangial and intramembranous locations (arrows), some with peaks (5,000X",yes
PMC6342896,Figure_4,oa_package/be/07/PMC6342896.tar.gz,"[' 4).', 'Irregular skin lesions of the arm produced by ant feeding activity on the cadaver.', 'Bassi)Post-mortem injuries caused by Formicidae [35, 36] can be easily misinterpreted as ante-mortem lesions, such as abrasions, cigarette or strong acids scars, patterned abrasion due to the imprinted effect of a blunt or offending object and marks from manual or even ligature strangulation, especially when located on the neck and restricted by the collar line of a T-shirt or pullover.']",Fig. 4 Irregular skin lesions of the arm produced by ant feeding activity on the cadaver. Ants can affect the interpretation of the tanatological data both removing fly larvae and producing post-mortem lesions orange-pink to yellow in colour diffusely scattered over the skin surface (Photo by ),yes
PMC4228332,Figure_6,oa_package/b0/5b/PMC4228332.tar.gz,"['495) (A).', 'Unexpectedly, we did not observe any significant changes in the number of bacteria between na ve and DSS-treated wild-type and PGRN / mice ( B E).', 'org/1999/xlink"" xlink:href=""srep07023-f5""/>Intestinal barrier function and the microbiota are not altered in PGRN / mice.']",Figure 6 Intestinal barrier function and the microbiota are not altered in / mice. (A) Intestinal permeability was examined by detection of a FITC-dextran in the serum from DSS-induced mice. (BE) Q-PCR analysis of intestinal microbiota in nave or DSS-treated wild-type and / mice. Values are shown as a relative ratio to total bacterial 16 s rDNA measured by 2ct method. All data represent means SE of three independent experiments. * < 0.05; ** < 0.01.,yes
PMC10520433,Figure_1,oa_package/e1/a5/PMC10520433.tar.gz,"['Computed tomography (CT) demonstrated a noncontrast-enhancing para-aortic fluid collection (Fig 1).', 'Fig 1Axial reconstruction of computed tomography angiography (CTA) of patient A demonstrating the proximal endograft in the infrarenal segment and para-aortic abscess (black arrow).']",Fig1 Axial reconstruction of computed tomography angiography (CTA) of patient A demonstrating the proximal endograft in the infrarenal segment and para-aortic abscess ( ).,yes
PMC3625775,Figure_1,oa_package/92/dd/PMC3625775.tar.gz,"['The cosmetic outcome was evaluated and scored by two physicians, a surgeon who carried out the operation and a radiation oncologist, based on the Harvard Breast Cosmesis Scale: excellent (almost identical to untreated breast), good (minimal difference between breasts, satisfactory symmetry and distortion), fair (obvious difference, acceptable asymmetry and distortion), and poor (major difference, noticeable asymmetry and distortion) () [20].', 'Therefore, though the cosmetic outcome may be scored as good, a lump may be palpable in the mesh implanted breast (A).', 'Long-term cosmetic resultsArch Surg1989124153157291693521KlingeUSchumpelickVKlosterhalfenBFunctional assessment and tissue response of short- and long-term absorbable surgical meshesBiomaterials200122141514241133631622KimHOHwangSIYomCKParkYLBaeWGThe use of absorbable surgical mesh after partial mastectomy for improving the cosmetic outcomeJ Breast Cancer20091215115523BourneRBBitarHAndreaePRMartinLMFinlayJBMarquisFIn-vivo comparison of four absorbable sutures: Vicryl, Dexon Plus, Maxon and PDSCan J Surg1988314345282787524EomTIKimBSKooBYKimJWLimYALeeHHThe use of a corrective procedure with Vicryl mesh for oncoplastic surgery of the breastJ Breast Cancer200912364025G esJCLandeckerALyraECHenr quezLJG esRSGodoyPMThe application of mesh support in periareolar breast surgery: clinical and mammographic evaluationAesthetic Plast Surg2004282682741566604226G esJCPeriareolar mammaplasty: double skin technique with application of polyglactine or mixed meshPlast Reconstr Surg199697959968861899927WhitfieldGAHoranGIrwinMSMalataCMWishartGCWilsonCBIncidence of severe capsular contracture following implant-based immediate breast reconstruction with or without postoperative chest wall radiotherapy using 40 Gray in 15 fractionsRadiother Oncol2009901411471897754728Hill-KayserCEVachaniCHampshireMKDi LulloGAMetzJMCosmetic outcomes and complications reported by patients having undergone breast-conserving treatmentInt J Radiat Oncol Biol Phys2012838398442213702229LiviLBorghesiSSaievaCMeattiniIRampiniAPetrucciARadiotherapy timing in 4,820 patients with breast cancer: university of florence experienceInt J Radiat Oncol Biol Phys20097336536918715726Cosmetic outcomes and sonographic findings.']","Figure 1 Cosmetic outcomes and sonographic findings. (A, D) Good cosmetic outcome. Sonography indicated echogenic materials (in a 51-year-old woman with infiltrating ductal carcinoma [IDC] at 20 months after operation). (B, E) Fair cosmetic result showed mild asymmetry and nipple deviation, but no contracture. Sonography indicated parenchymal distortion (in a 42-year-old woman with IDC at 24 months after operation). (C, F) Poor cosmetic result was expressed by severe contracture, asymmetry and pain. Sonography indicated seroma (in a 54-year-old woman with IDC at 18 months after operation).",yes
PMC9683396,Figure_4,oa_package/81/ba/PMC9683396.tar.gz,"['Neither p-tau 217 nor p-tau 231 was detected in the LC of all genotypes, suggesting that either ageing or A pathology in the cortex was not sufficient to induce tau phosphorylation at these sites in the LC (Supplementary A and B).', 'To determine whether p-tau 181 and total tau were accumulated and formed tau pathology in the noradrenergic neurons of the LC, brain sections of aged AppNLGF, AppNL and WT mice were stained with antibodies against p-tau 181 and total tau, but there were no differences in their staining patterns in any of these mice (Supplementary C and D).', 'None of these marker proteins colocalized with p-tau 202/205/208 (AT8), although a cross-sectional view from 3D reconstruction revealed that p-tau 202/205/208 (AT8) signals were often adjacent to those of Bassoon, a marker for presynaptic terminals (B).', '\nColocalization of p-tau 217, 231 and 202/205/208 (AT8) with a postsynaptic marker, PSD95.', 'Although the signals of p-tau 202/205/208 (AT8) and MAP2 did not clearly overlap (D), p-tau 202/205/208 (AT8) and PSD95 signals showed colocalization (', 'A cross-sectional view of a 3D reconstruction confirmed that p-tau 202/205/208 (AT8) and PSD95 signals overlapped (C) and were often adjacent to MAP2 signals (', '5% of p-tau 202/205/208 (AT8)-positive punctate signals colocalized with PSD95 (A and E).', '7% of p-tau 181 signals did (F).', 'The p-tau 181 signals were well colocalized with NfL signals in all genotypes (G, inset with orange squares), and these structures were disrupted around A plaques in AppNLGF mice (', 'In contrast, p-tau 231-positive punctate signals were not colocalized with NfL, as expected (H).']","Figure 3 Representative images of cortices from frozen coronal brain sections immunostained with ( ) antibodies against p-tau (217, 202/205/208:AT8 or 181) and LAMP1, a marker of dystrophic neurites. A plaques are stained with FSB. ( ) antibodies against p-tau AT8 and the glutamatergic presynaptic marker VGLUT1, the GABAergic presynaptic marker VGAT, the cholinergic terminal marker VAChT or the serotonergic terminal marker 5-HT. Scale bars, 20m in each left panel. Each right panel shows a higher magnification view of the corresponding dashed orange squares; scale bars, 2.5m.",yes
PMC6749096,Figure_4,oa_package/28/da/PMC6749096.tar.gz,"['5 fold higher than control adrenal tissue), but not in the left gland, which was mainly composed of myelolipoma ().', ""In the patient's BMAH/myelolipomas tissues, no increased expression of LHCGR nor GnRHR was found, despite in vivo stimulation of cortisol by LH ()."", 'Messenger RNA (mRNA) expression levels for the receptors of GIP, LHCG, GNRH expression compared to control (pool of 5 control adrenals from Clontech) in the left and right adrenal gland tissue extracts of the patient, as determined by real-time quantitative PCR.']","Figure 4 Messenger RNA (mRNA) expression levels for the receptors of GIP, LHCG, GNRH expression compared to control (pool of 5 control adrenals from Clontech) in the left and right adrenal gland tissue extracts of the patient, as determined by real-time quantitative PCR. When compared to pool of normal adrenal glands, GIPR overexpression was found by RT-PCR in the right adrenal gland [10.5 fold higher than control adrenal tissue (*** < 0.05)]. In the patient's BMAH/myelolipomas tissues in the left adrenal gland no increased expression of GIPR was found (* > 0.05) and reduced expression of LHCGR and GnRHR (** < 0.05) were found when comparing with those in the control tissues.",yes
PMC9715374,Figure_5,oa_package/43/8d/PMC9715374.tar.gz,"['8 and 11 [], and 12), in which the lesions increased in size (Supplementary Table 1), as was seen in the IOPAR observations.', 'Increased lesion size and gray values in No.', '11 in PRP group, with an increase in lesion size (, Supplementary Table 1).']","Figure 5 Increased lesion size and gray values in No. 11 of the platelet-rich plasma group. HU, Hounsfield unit; SD, standard deviation.",yes
PMC10339097,Figure_3,oa_package/79/58/PMC10339097.tar.gz,"['"" id=""jbm410754-fig-0003"">Physiologic response to leptin infusion in F344BN F1 hybrid rats.']","Fig. 3 Physiologic response to leptin infusion in F344BN F1 hybrid rats. (A) Plasma leptin concentrations before (pooled baseline) or after insertion of miniosmotic pumps filled with 1g/mL recombinant rat leptin (leptin pump) or saline (saline pump). Arrows show timing of four serial pump replacements over a 23week period (i.e., 35 to 42day intervals). Based on a diffusion rate of 0.15L/h, leptin pump rats received 3.6g leptin per day, which significantly increased plasma leptin over time. values show post hoc agematched paired comparisons between leptin and saline pump groups. Data points represent mean95% CI ( =8 per group). (B) Weekly body weights did not differ between rats in saline and leptin pump groups. Data points represent mean95% CI ( =8 per group). (C) Average daily food consumption was modestly lower in leptin pump rats versus saline pump rats following third and fourth osmotic pump replacements. Data were collected over a 5day period to generate a daily average at indicated intervals. values show post hoc agematched paired comparisons between leptin ( =8) and saline ( =8) pump groups. (D) Tricep muscle mass was measured by gross dissection following euthanasia and was not altered by leptin infusion. Individual animal data are shown as closed circles. Boxes represent 25th to 75th percentiles, horizontal line indicates median, and whiskers span minimum to maximum values. (E) Gonadal fat pad mass, which was measured by gross dissection following euthanasia, was also similar between leptin and salinetreated animals. (F) Nonperitoneal adipose tissue volume was quantified by MRI 16weeks after initiation of leptin infusion. Data are reported as fraction of adipose to nonadipose tissue volume using watersuppressed image sequences and automated segmentation procedures of userdefined anatomic regions. No differences were observed between leptin and salinetreated groups.",yes
PMC6831494,Figure_2,oa_package/e5/77/PMC6831494.tar.gz,"['b, d, and\nf.', 'The results of the quantitative\nanalysis showed that the area (number) of the bile duct increased in correlation with the\ndegree of bile duct proliferation graded by pathologists (g).']","Fig. 2. Quantification of the bile duct area in the liver. (a, c, e) Original images. (b,d, f) Segmented images of bile duct (green), infiltrating cells (light blue), redblood cells (orange), and other regions (pink) using the tissue classifier module.Grade of proliferation of bile duct evaluated by pathologists: (a, b) no change, ;(c, d) slight, +; (e, f) moderate, ++. Bar = 100 m. (g) Quantitative results forbile duct area (% of the whole area of the liver section).",yes
PMC8544166,Figure_5,oa_package/e3/f3/PMC8544166.tar.gz,"['Mineralization, Aorta/Medial or Mural Artery(.', 'Mineralization(55.', 'Vacuolation(66.']","Figure 5. Aorta, Mineralization, H&E.",yes
PMC10401014,Figure_4,oa_package/63/f9/PMC10401014.tar.gz,[],"Fig. 4. DSF modifies Cys133 of MD-2. ( and ) iBMDMs were pretreated with the indicated doses of DSF in the presence or absence of Tiron ( ) or N-acetyl-L-cysteine (NAC) ( ) for 0.5 h before stimulation or not with LPS (1 g/mL) for 4 h. mRNA levels of the indicated inflammatory cytokines were assessed by qRT-PCR, normalized to and relative to unstimulated cells. ( ) , Tiron 1 mM vs. 0 mM = 0.0005, 2 mM vs. 0 mM = 0.0029; , Tiron 1 mM vs. 0 mM = 0.0012, 2 mM vs. 0 mM < 0.0001, , Tiron 1mM vs 0 mM <0.0001, 2 mM vs. 0 mM < 0.0001; ( ) , NAC 1 mM vs. 0 mM = 0.0105, 2 mM vs. 0 mM = 0.0012; , NAC 1 mM vs. 0 mM < 0.0001, 2 mM vs. 0 mM < 0.0001, , NAC 1 mM vs. 0 mM < 0.001, 2 mM vs. 0 mM < 0.0001. ( ) Recombinant mouse MD-2 protein was incubated with DSF or DMSO for 1 h before mass spectrometry analysis. Cys133 on MD-2 modification by DSF is highlighted. The mass-tocharge ratio (m/z), nuclear charge (z), and theoretical masses of indicated peptides are shown in red. The spectra at the bottom plot relative abundance vs. m/z ratio. ( ) Quantitative analysis of internalization of Alexa Fluor 488-labeled LPS by iBMDMs pretreated or not with DSF using flow cytometry. ( ) Representative flow cytometry histograms showing TLR4/MD-2 complex expression on the surface of iBMDM cells treated or not with DSF. ( ) iBMDMs, pretreated with DSF for 0.5 h and extensively washed to remove DSF, were treated with increasing concentrations of recombinant MD-2 and stimulated with LPS for 4 h before measuring inflammatory cytokine mRNA levels by qRT-PCR. mRNA level was normalized to and relative to unstimulated cells. Graphs in , , and show mean SD; data are representative of three independent experiments. Data were analyzed using two-way ANOVA ( and ) or a two-tailed Students test ( ). * < 0.05; ** < 0.01; *** < 0.001.",yes
PMC8955356,Figure_9,oa_package/c0/d9/PMC8955356.tar.gz,"['The other augmentation techniques (see ) applied to the dataset include multiplication, which multiplies all pixels in an image by a random value sampled uniformly from the interval [0.', 'Augmentation applied to images; (a) original image (b) contrasted image (c) multiplied image (d) blurred image (e) sharpened image.']",Figure 9 Augmentation applied to images; ( ) original image ( ) contrasted image ( ) multiplied image ( ) blurred image ( ) sharpened image.,yes
PMC9929755,Figure_1,oa_package/88/9c/PMC9929755.tar.gz,"['He had some retinal pallor and edema in the posterior pole and cherry red spot (CRS) appeared in the fovea ().', 'Wide-field fundus photography of the patient s right eye.']","Figure 1 Wide-field fundus photography of the patients right eye. His right eye had a clear optic disc, and his C/D was about 0.3. His retinal arteries were thinned, A:V was about 1:2. He had some retinal pallor and edema in the posterior pole and CRS was observed in the fovea. C/D, cup to disk ratio; A:V, arterial/venous; CRS, cherry red spot.",yes
PMC5319922,Figure_1,oa_package/26/6a/PMC5319922.tar.gz,"['The selected fiber tracts were identified within the probabilistic JHU fiber tract ROI atlas, and the connected brain areas in the DMN were determined as illustrated in ', 'The thus produced DMN mask was superimposed onto the JHU fiber tract atlas in MNI space (', ""The intersection between the dilated fiber tracts and the DMN mask defined then the tract's cortical projection area ("", 'To provide larger coverage of the projection area within the DMN, the intersecting region was dilated by another 6 mm within the boundaries of the DMN mask, thus yielding the final DMN ROI for a particular tract (', ""The ROI was used to extract the mean ROI value from a subject's DMN map for each tract, which was then analyzed in relation to the WMH volume within the same tract ("", '']","Fig.1 Schema of successive steps involved in generating the default mode network (DMN) regions of interest (ROI) connected by a tract (here, the cingulum) and the WMH located in that tract. A three-dimensional rendering of the location of the cingulum (red) and the DMN (blue) is shown (A, left panel), where after different processing steps, the final cingulum-connected ROI of the DMN (green) and the WMH (yellow) located in that fiber tract ROI are yielded (A, right panel). (BE) Illustrate the different processing steps to produce those final ROIs. In the first step, the DMN mask (blue) obtained from a previously published DMN template and the fiber tract mask (red) obtained from the probabilistic JHU fiber tract atlas are fused in MNI space (B). Next, the fiber tract was dilated by 6mm (meshed red) to determine the area of spatial overlap between the tract and the DMN mask (green, [C]). The green area was dilated in another iteration by 6mm within the boundaries of the DMN, thus yielding a larger projection area of the fiber tract within the DMN (white arrows pointing to extended green area outside the red meshed sphere, D). This was done to ensure sufficient coverage of the DMN for a representative sampling of DMN FC values within the projection area of the tract. Finally, the spatially normalized WMH map (yellow) was superimposed onto the fiber tract, and the WMH volume within the fiber tract was calculated (E).",yes
PMC7394107,Figure_5,oa_package/f2/0c/PMC7394107.tar.gz,"['In punch elevation or punch flotation, the depressed scar is lifted from its bed by breaking the adhesions, which bind down the scar [].', 'Punch elevation in grade 4 acne scarsIf the surface of the scar is atrophic and it is less than 3.']",Figure 5 Punch elevation in grade 4 acne scars,yes
PMC5585498,Figure_9,oa_package/7a/2e/PMC5585498.tar.gz,['HE staining.'],"Figure 9 HE staining. Infiltration of abundant inflammatory cells and atypical lymphoid cells (ALC) arranged in an angiocentric pattern. A, HE staining (20) with dilated vessels observed (black arrow heads). B, HE staining (40) showing the infiltration of small-to-medium sized cells with irregular nuclei.",yes
PMC5166487,Figure_7,oa_package/76/98/PMC5166487.tar.gz,"[' Representative micrographs of immunohistochemical technique against iNOS antibody in spleen, thymus and lungs from BTV-4-infected and Mock animals.']","Figure 7 The number of iNOS+ cells was highly increased in spleen from BTV-infected animals and these cells were mainly located in necrotic areas surrounding the white pulp (asterisk). In the thymus of infected mice, immunoreactivity against iNOS antibody was higher than mock-infected individuals and was randomly distributed throughout the parenchyma. A few number of iNOS+ cells was present in the inflammatory infiltrate of the lung of infected mice.",yes
PMC10321170,Figure_2,oa_package/de/c3/PMC10321170.tar.gz,['Shear wave velocity measurement in a patient with SCA.'],"Figure 2 Shear wave velocity measurement in a patient with SCA. Parenchymal stiffness measured in the ROI on the left lobe was 11.45 kPa. ROI, region of interest; SCA, sickle cell anemia.",yes
PMC9436684,Figure_2,oa_package/a2/b5/PMC9436684.tar.gz,"['"" id=""iju512493-fig-0002"">The pathologic findings of the needle biopsy core.']","Fig. 2 The pathologic findings of the needle biopsy core. Magnification, 100. Hematoxylin and eosin staining.",yes
PMC2992046,Figure_4,oa_package/d6/83/PMC2992046.tar.gz,['Using Luminex Multiplex Bead Arrays to Measure Intracellular PD Markers is More Sensitive than Conventional Western Blot Analysis.'],"Figure 4 . Using the 60 day CIA mouse paws we extracted the paw supernatants as described in the materials and methods and tested for phospho-STAT3 using either conventional western blot analysis with densitometry or multiplex bead analysis. Extracts from two different mice per group were run on a SDS-PAGE gel and transferred onto a nitrocellulose membrane and exposed to film for the final image. Western blot was probed with anti-pSTAT3 (79 kDa) and (86 kDa) subunits, stripped then re-probed for total STAT3, (76 kD) and (86 kDa) subunits as shown. Below graph shows densitometry analysis from above blot **ns p = 0.2037 versus vehicle, *ns p = 0.1226 versus CIA + vehicle group, N = 3 per group shown. Luminex phospho-STAT kit analyzed paw extractions from CIA mice treated with vehicle alone or dexamethasone. Samples were those same as used for the W.blot above, *p = 0.034 compared CIA + vehicle group, **p = 0.017 compared to vehicle alone. For both graphs, statistical test used for p-values: two-tailed paired Student's t-test, N = 3-4 mice per group shown. All graphs show Mean SEM, ns = not significant.",yes
PMC10466011,Figure_4,oa_package/90/31/PMC10466011.tar.gz,"['()S-100 immunostain showed strong, diffuse deposition of nuclear and cytoplasmic brown, confirming the presence of a neural tumor of Schwann cell origin.', 'The blue-colored center of rosettes and the periphery of the tumor confirm the presence of collagen fibers (MT staining, 4).']","Fig. 4 The blue-colored center of rosettes and the periphery of the tumor confirm the presence of collagen fibers (MT staining, 4).",yes
PMC7394136,Figure_5,oa_package/e4/0b/PMC7394136.tar.gz,"['A sinogram was performed by injecting contrast through the catheter, showing passage into the ascending colon, confirming the initial diagnosis ().', 'Sinogram obtained by anterioposterior x-ray of segment of lower chest/abdomen following injection of contrast into the percutaneous pigtail catheter, demonstrating passage of contrast into the hepatic flexure of the colon via an apparent fistulous connection.']","Figure 5 Sinogram obtained by anterioposterior x-ray of segment of lower chest/abdomen following injection of contrast into the percutaneous pigtail catheter, demonstrating passage of contrast into the hepatic flexure of the colon via an apparent fistulous connection.",yes
PMC10740299,Figure_1,oa_package/77/87/PMC10740299.tar.gz,"[' 1A).', ' 1B).', '', 'Statistical analysis was conducted by Student s t testTo determine the relationship between cell types, we plotted the data across the three tissue types in patients with NMOSD and HCs.', ' 1C).', '1D).', '1E).', 'We found that BTK, BCL2, PIK3CA and CD40 were up-regulated and mRNA of LYN was decreased in NMOSD (', ' 1G, H).', 'Since the level of pBTK fluctuated among patients, we compared the ratio of pBTK stim/pBTKunstim between HCs and NMOSD patients and found that this ratio was significantly increased in total blood B-cell na ve B cells and class-switched memory B cells in NMOSD (', ' 1I).', ' 1J).']","Fig.1 Single-cell RNA-sequencing and flow cytometry characterization of BTK expression in NMOSD. Visualization of clustering by t-distributed stochastic neighbor embedding (t-SNE) plot of all B cells obtained from blood, CSF and bone marrow. Heat map of scaled gene expression for top ten differentially expressed genes identifying each cluster, with selected genes listed. Average proportion of each cell type derived from healthy controls (HCs, blood, =3; bone marrow, =2) and NMOSD (blood, =6; bone marrow, =2; CSF, =4) samples. Volcano plot of differential expression genes of B cells between blood of patients with NMOSD and HCs or CSF/bone marrow from patients with NMOSD. Green, downregulated; red, upregulated. Heatmap of differential expression genes (DEGs) involving -related genes in B cells from different sources. Expression of BCR signaling-related genes mRNA in peripheral blood B cells from HCs and patients with NMOSD was analyzed using real-time PCR. (HCs =9 and NMOSD, =18); (HCs, =8 and NMOSD, =15); (HCs, =6 and NMOSD, =15), (HCs, =5 and NMOSD, =15) and (HCs, =7 and NMOSD, =15). Representative flow plots show BTK protein expression in a NMOSD patient, a healthy control, and a fluorescence minus one (FMO). BTK protein expression was measured by intracellular flow cytometry in total B cells and B-cell subpopulations in HCs and NMOSD patients, normalized to BTK expression in total B cells of HCs, which was set to 1.0. ( =16 per group). Phospho-BTK (pBTK) expression in total B cells and the indicated B-cell subpopulations that were either left unstimulated (pBTK ) or were stimulated (pBTK ) for 30s with anti-IgM (20g/ml) in blood of NMOSD patients and HCs ( =16 per group) or in blood and CSF from NMOSD patients ( (blood, =16; CSF, =6). Data are displayed as meansSEM. Statistical analysis was conducted by Students t test",yes
PMC3524726,Figure_1,oa_package/bf/d3/PMC3524726.tar.gz,"['Although headache was also the most common symptom in the study group, sometimes serious pathology of the sphenoid sinus remained totally asymptomatic until complications emerged ().', 'A review of 30 casesN Engl J Med1983309114954662166114PearlmanSJLawsonWBillerHFIsolated sphenoid sinus diseaseLaryngoscope19899971620274739515G ven MGKaytazAOzbilen AcarGAdaMCurrent management of isolated sphenoiditisEur Arch Otorhinolaryngol2009266987921905276516BatraPSCitardiMJLanzaDCIsolated sphenoid sinusitis after transsphenoidal hypophysectomyAm J Rhinol200519185891592121917NicholasBDBhargaveGHatipogluAPreoperative prevalence of methicillin-resistant Staphylococcus aureus (MRSA) colonization in patients undergoing intranasal surgeryMed Sci Monit2010168CR365682067161218SantamariaFDe StefanoSMontellaSNasal nitric oxide assessment in primary ciliary dyskinesia using aspiration, exhalation, and hummingMed Sci Monit2008142CR808518227765Meningocele the cause of recurrent meningitis CT, axial plane.']","Figure 1 Meningocele the cause of recurrent meningitis CT, axial plane.",yes
PMC10999957,Figure_1,oa_package/23/9e/PMC10999957.tar.gz,['\nCraneocaudal and Mediolateral Oblique projections of mammography.'],"Figure 1 The breast tissue is extremely dense (category D of the American College of Radiology, 2013). In the right breast exists a conglomerate of nodules (arrow) localized at the third posterior of the union of lower quadrants, they are two isodense and oval nodules with obscured margins.",yes
PMC3095625,Figure_4,oa_package/b8/53/PMC3095625.tar.gz,"['We determined that induction of FRG1-FLAG in iC2C12-FRG1 myoblasts has a negative effect on their proliferation rate, as shown by the increased doubling time when grown in doxycycline (\nA\n).', 'g004Characterization of proliferation defect in iC2C12-FRG1 myoblasts.', 'Subsequent flow cytometry analysis of the cells for DNA content indicated an increased fraction of FRG1 overexpressing iC2C12-FRG1 myoblasts in G1-phase, coupled with a corresponding decrease in the fraction of cells in S-phase (\nA\n).', 'We observed that FRG1 overexpressing cells exhibited a consistent 1 2 hour delay in exit from G1 and entry into S-phase (\nB\n\n \n\nTable 2\n).', 'One disparity in our data may be the difference observed between the proliferative defect described in mass culture (A) and that seen by mitotic shakeoff analyzed by flow cytometry (B).']",10.1371/journal.pone.0019780.g004,yes
PMC10724056,Figure_4,oa_package/e3/f3/PMC10724056.tar.gz,"['Interestingly, in SORL1+/ and in two of the variant lines (Y141C and E270K) we did not observe enlarged lysosomes (s 4A and 4B).', 'However, in a different variant cell line, G511R, we did document a significantly increased lysosome size that is reduced after TPT-260 treatment (s 4C and 4D).', 'In TPT-260 treated neurons, we document a complete rescue of this phenotype (s 4E and 4F), suggesting that retromer enhancement can promote this pathway, even in the absence of SORL1.', ' 4Retromer enhancement with TPT-260 rescues enlarged endosome phenotype in G511R+/ hiPSC-derived neurons and enhances lysosomal degradation in SORL1 KO neurons(A and B) Lysosome size measured using lysosome-specific marker LAMP1 in WT, SORL1Var, and SORL1+/ hiPSC-derived neurons.', 'In this study, we found that only one variant of SORL1, G511R, demonstrated enlarged lysosomes and that this phenotype was rescued with TPT-260 (s 4C and 4D).', 'When we probed further into the effects of retromer enhancement on lysosomal degradation using the DQ-Red-BSA assay, we observed that TPT-260 enhances lysosomal degradation in SORL1 KO cells, bringing this function to WT levels (s 4D and 4E).', 'This is the second example of phenotypic differences between variants in our study, as only G511R variant cells showed changes in lysosomal morphology (s 4C and 4D).']","Figure3 Retromer enhancement with TPT-260 reduces AD pathological phenotypes (A and B) Levels of secreted A 140 (A)and A 142 (B)are reduced with TPT-260 treatment in all genotypes, compared with DMSO, except secreted A 142 in KO neurons (indicated by asterisks). The % decrease in A 140 and A 142 levels of TPT-260-treated cells is greater in and neurons as compared with KO neurons (indicated by hashmarks). For all experiments, one to three clones and three replicates per clone per genotype were used. Each data point represents A levels measured from the media per clone/per well. Data represented as mean SD. (CE) TAU phosphorylation on three epitopes was examined in response to TPT-260 treatment. (C) Thr 231 epitope levels, as measured by ELISA assay, is reduced in all the genotypes treated with TPT-260 relative to WT DMSO controls. Each datapoint represents total TAU or phospho-TAU levels measured from the cell lysate per clone/per well. (D) The PHF-1 (Ser396/Ser404) and the (E) AT8(Ser202/Thr305) epitopes as measured by western blot is decreased in TPT-260 treated KO hiPSC-derived neurons relative to WT DMSO controls. One to three clones were analyzed per genotype. Each datapoint represents total TAU or phospho-TAU levels measured from the cell lysate per clone/per well. Data represented as mean SD. Normally distributed data were analyzed using parametric two-way ANOVA. One to three clones were analyzed per genotype. Significance was defined as a value of / p<0.05, / p<0.01, / p<0.001, and / p<0.0001. ns, not significant.",yes
PMC9428845,Figure_1,oa_package/3e/45/PMC9428845.tar.gz,"['66 ng/mLAbdomen and pelvic CT scans (, ) were performed without IV contrast given his acute kidney injury.', 'Coronal (a) and sagittal (b) views showing fecal impaction of the splenic flexure and descending colon.', '']",Fig. 1 Coronal (a) and sagittal (b) views showing fecal impaction of the splenic flexure and descending colon. Wall thickening can be appreciated in both images.,yes
PMC8718151,Figure_1,oa_package/41/1c/PMC8718151.tar.gz,"['Hierarchical clustering demonstrated samples from different experimental groups (sham, 1-day, and 7-day post-AMI groups) to be well separated from each other, while biological replicates from the same group clustered together well (Supplemental A; supplemental material available online with this article; Laparoscopic resection of the left subdiaphragmatic mass was performed under general\nanaesthesia on 28 June 2022.']",Figure 1. Computed tomography images of a haemangioma of the diaphragm found in a45-year-old male patient that was admitted to hospital after the incidentalidentification of a left subdiaphragmatic mass during a preoperative chestcomputed tomography examination. The arrows show the haemangioma.,yes
PMC8613281,Figure_4,oa_package/fb/97/PMC8613281.tar.gz,"[' 4A).', ' 4B).', ' 4B).', 'Chronic gOHT results in optic nerve head tissue remodeling and neuroinflammation.', 'Eye growth does not contribute to increased IOP in the gOHT modelWe were curious as to the mechanism underlying the gradual suture tightening over time and evaluated eye growth as a potential explanation.', ' 4B), we also compared this signal in the two models.']","Figure 4 Chronic gOHT results in optic nerve head tissue remodeling and neuroinflammation. ( ) Representative phalloidin staining (red) highlights an intact pseudolaminar region in control (CON) tissue (arrows), which was disrupted in gOHT eyes (scale bar indicates 50m). ( ) Representative immunostaining panels of CON and gOHT in sections of optic nerve head tissue. For orientation of all images, the white arrow indicates DAPI stained retinal layers on either side, * indicates the vitreous cavity adjacent to optic nerve head, and # indicates the optic nerve). There was mildly increased staining for MMP-9, and strongly increased CD68 in gOHT compared to CON tissues. There were no notable differences for any other marker (scale bar indicates 50m).",yes
PMC10540831,Figure_8,oa_package/e4/e4/PMC10540831.tar.gz,[],"FIGURE 8 Pain markers: (A) nerve growth factor ( ) and (B) brainderived neurotrophic factor ( ) gene expression normalized to respective SHAM controls for Scleraxis ( ) and Mohawk ( ) transfected cells at 48h, 7days, and 14days, respectively, for (right) autopsy and (left) surgical cells. <0.05 compared to SHAM (pCMV6), * <0.05.",yes
PMC11191389,Figure_6,oa_package/4d/45/PMC11191389.tar.gz,[],Figure 5 First obtuse marginal artery with two drug-eluting stents placed,yes
PMC4689401,Figure_4,oa_package/f0/76/PMC4689401.tar.gz,"['A) Four and one-half months post-infection, Western blots developed with mouse monoclonal anti-I1\nPP2A (5G6) showed a significant increase in the level of the transgene (normalized with actin as loading control; actin blot the same as shown in A) in the ventricular area, the cerebellum, the cerebral cortex and the hippocampus in AAV1-I1\nPP2A rats as compared with that in AAV1-GFP control rats.', 'We found that tau hyperphosphorylation at Ser-199, Thr205, Thr212, Ser-214, Thr231/Ser-235, Ser-262/Ser-356, Ser-396 and Ser-422 in the ventricular area, the cerebellum, the cerebral cortex and the hippocampus of AAV1-I1\nPP2A rats, was markedly increased when compared with AAV1-GFP control animals (A and 4B).', 'g004Memantine inhibits I1\nPP2A-induced tau hyperphosphorylation.', 'g005""/>Memantine treatment markedly inhibited tau hyperphosphorylation except some tau sites, which showed a trend of decrease (A and 4B).']",10.1371/journal.pone.0145441.g004,yes
PMC10640698,Figure_2,oa_package/87/20/PMC10640698.tar.gz,['Standard echocardiographic views are used to assess a pericardial effusion.'],"Figure 2 Standard echocardiographic views are used to assess a pericardial effusion. As seen in the figure above, the pericardial effusion is impeding normal myocardial contractility. The emergent transthoracic echocardiography (TTE) demonstrated a large pericardial effusion measuring 5.4 cm posterior to the left ventricle.",yes
PMC5648881,Figure_2,oa_package/70/cf/PMC5648881.tar.gz,"['\nMorphology and structure of stromal striae in normal corneaStriae in normal cornea were few, short and oblique (']","Figure 2 Striae in corneas with edema: Fuchs dystrophy (left panels), bullous keratopathy (right panels). Viewed with (clockwise) confocal microscopy (CM), OCT, FFOCM cross-sectional xz and en face xy views. Arrowheads indicate striae. Striae in edematous corneas were few and oblique. Scale bars show 100m.",yes
PMC8393596,Figure_1,oa_package/15/14/PMC8393596.tar.gz,"['The endometriotic 12-Z cell line was treated with TNF or IL1 at 10 ng mL 1 for 48 h (A).', '1) revealed 437 and 35 significantly regulated proteins after treatment with TNF or IL1 , respectively, while 27 protein groups were regulated in both treatments (B).', 'The most prominent regulations confirmed a successful inflammatory stimulation of the cells by upregulating IL-12 and NF- B signaling pathways (A).', ', SOD2, SERPINB2) as exemplified in the heatmap in C.', 'We found a strikingly similar protein signature corresponding to this intermediate monocyte state for the TNF -treated, but not the IL1 -treated 12-Z cells (D).', '1177/193371911039127921160085(A) Schematic representation of endometriotic 12-Z cells treated with TNF (red) or IL1 (blue).']","Figure 1 ( ) Schematic representation of endometriotic 12-Z cells treated with TNF (red) or IL1 (blue). IL-12 and NF-B signaling was induced in both treatments while neuroangiogenesis was much more pronounced for TNF stimulation. ( ) Venn-Diagram showing the number of significantly regulated proteins (FDR = 0.05, S0 = 0.1) for TNF (red) and IL1 (blue) compared to the control state. Twenty-seven protein groups were significantly regulated in both treatments. ( ) Heatmap highlighting proteins involved in NF-B and Il-12 signaling and downstream targets. ( ) Protein signature characteristic for the phenotype of intermediate monocytes upon treatment of epithelial-like 12-Z cells with TNF (red) and not with IL1 (blue). Asterisks (*) show multi-parameter corrected significant regulations of protein abundance (FDR = 0.05, S0 = 0.1) compared to untreated controls (Con).",yes
PMC2740225,Figure_1,oa_package/f5/d6/PMC2740225.tar.gz,"['Abdominal computed tomography (CT) showed ascites, splenomegaly and heterogeneous liver nodules, filled with contrast-like agent ().', '.']","Figure 1. First abdominal computed tomography of our patient showing ascites and diffuse heterogeneous liver nodules, filled with contrast-like agent.",yes
PMC6716316,Figure_1,oa_package/3b/52/PMC6716316.tar.gz,"['8 mm [].', 'Coronary angiogram showing the coronary artery fistula originating from the left main coronary artery.']","Figure 1 Coronary angiogram showing the coronary artery fistula originating from the left main coronary artery. LAD: Left anterior descending artery, LCX: Left circumflex artery",yes
PMC6269335,Figure_18,oa_package/0c/9a/PMC6269335.tar.gz,[],Fig. 18 Malignant transformation of the fibrous dysplasia (FD) lesion. A patient with known craniofacial FD ( ) presented with enlarging left jaw mass ( ). CT of the facial bones demonstrated an aggressive lytic lesion with soft tissue component involving the left aspect of the mandible ( ) ( ). The pathology showed malignant transformation of the mandibular FD lesion,yes
PMC11318702,Figure_4,oa_package/09/34/PMC11318702.tar.gz,['.'],Figure 4. Hematoxylin and eosin stain (100) showing nests and sheets of well-differentiated neuroendocrine tumor.,yes
PMC5641442,Figure_2,oa_package/d4/ee/PMC5641442.tar.gz,['.'],"Figure 2. A: Abnormal uterine position on HSG shows a spindle shaped uterine cavity deviated towards left with patent left fallopian tube. Right fallopian tube is not seen. Findings are suggestive of a unicornuate uterus on HSG. B:VRT image from MDCT-HSG shows a normal uterine cavity, right terminal hydrosalpinx with fimbrial block, left fallopian tube is patent. However, non-filling of right tube led to the misinterpretation of a unicornuate uterus on HSG",yes
PMC8600730,Figure_1,oa_package/fa/7f/PMC8600730.tar.gz,"[' 1).', 'Magnetic resonance imaging of a 22-year- old woman with choriocarcinoma.', 'Tortuous flow voids consistent with vessels can be observed in the parametrium, indicating tumor hypervascularityWhile profuse bleeding may occur after biopsy, a histological diagnosis is important and may be lifesaving for patients with any of the aforementioned conditions.']","Fig. 1 Magnetic resonance imaging of a 22-year- old woman with choriocarcinoma. Sagittal T2-weighted image ( ) and axial T2-weighted image ( ) demonstrate a heterogeneous, hyperintense, focal mass located in the posterior cervical lip. Coronal ( ) contrast-enhanced T1-weighted images show heterogeneous enhancement of the tumor. Tortuous flow voids consistent with vessels can be observed in the parametrium, indicating tumor hypervascularity",yes
PMC4062494,Figure_4,oa_package/22/08/PMC4062494.tar.gz,"['05) (A).', 'However, less fibrous septa and collagen were found in BSE-CD-treated mice (A).', '05) (B).', 'g004Effect of late phase BSE-CD treatment on hepatic fibrosis of liver tissue from mice that had been infected with S.', 'Mice were treated as described in .', 'japonicum egg-induced fibrosis ().', 'Our data show that BSE-CD could markedly reduce HSC activation (), which is expected to contribute to the suppression of hepatic fibrosis formation.']",10.1371/journal.pone.0100129.g004,yes
PMC11388015,Figure_1,oa_package/2c/64/PMC11388015.tar.gz,"['No light spot separation was seen in the collecting system of both kidneys (A).', 'Enhanced urinary CT detected a cystic low density shadow in the right kidney (B).', 'Enhanced CT of the cyst at arterial phase was about 17 Hu (C).', 'Preoperative imaging images.', '3Treatment and outcomePreoperative diagnosis of the patient was right renal cyst, and laparoscopic decompression of the right renal cyst was performed.']","Fig. 1 Preoperative imaging images. A. Right kidney color ultrasound examination of low density cystic shadow of right kidney. B. CT scan of the kidney showed a cystic shadow of the right kidney, most of which protruded from the kidney surface. C. Enhanced CT examination of the kidney revealed cyst compartmentation and weak enhancement of the cyst wall. Right renal cyst was classified as Bosinak II grade F.",yes
PMC10536253,Figure_5,oa_package/a7/51/PMC10536253.tar.gz,['Pulmonary parenchyma consolidation infiltration and alveolar septal infiltration observed as well as interstitial pneumonia manifested by alveolitis ().'],"FIGURE 5 Lung pathology was of greater severity in donor and direct contact ferrets in the A/aquatic bird/South Korea/A/SW1/18/2018 (A/SW1/18) group compared to the A/California/04/2009 (A/CA/04) group. Compared to direct contact ferrets in the PBS groups , lung pathology in the A/SW1/18 groups was more severe, characterized by hematoxylin and eosin staining and immunohistochemical staining with monoclonal antibody of influenza A virus N protein. Pathology in the A/CA/04 groups was more severe compared to the PBS groups but less severe compared to the A/SW1/18 groups and was characterized by hematoxylin and eosin staining. Compared to respiratory contact ferrets, lung pathology in the A/CA/04 groups was only severe compared to the PBS and A/SW1/18 groups groups, characterized by hematoxylin and eosin staining and immunohistochemical staining with monoclonal antibody of influenza A virus N protein. Images are representative of = 3 ferrets. Magnification 100.",yes
PMC6054740,Figure_1,oa_package/d9/d8/PMC6054740.tar.gz,"[' 1); dystrophic neurites were less frequent [1, 11, 16, 17, 21, 26].', 'Morphology of poly-GR and aDMA pathology.', 'FCtx = frontal cortex, MCtx = Motor cortex, DF = dentate fascia, CA = hippocampal subfields, Cbl = cerebellar granular cell layerPoly-GR inclusion distribution among clinicopathologic subgroupsTo evaluate whether poly-GA, poly-GP or poly-GR pathology differs among different clinicopathologic subgroups, we quantified lesion burden with image analysis.', ' 1).', ' 1).', ' 1).']","Fig. 1 Morphology of poly-GR and aDMA pathology. Immunohistochemistry for poly-GR reveals neuronal cytoplasmic inclusions in frontal cortex ( ), CA2/3 ( ), CA4 ( ), DF ( ), and cerebellar granular layer ( ). Inclusions in glial cells were very rare ( ). Immunohistochemistry for aDMA shows neuronal cytoplasmic inclusions, as well as variable nuclear immunoreactivity, which was dense in some neurons ( ), moderate in others ( ) and minimal in others ( ); inclusions in CA2/3 ( ), DF ( ) and ( ) cerebellar granular layer ( ); neuronal nuclear inclusions (NII) ( ); and rare glial cytoplasmic inclusions ( ). Scale bar represents 20M. FCtx=frontal cortex, MCtx=Motor cortex, DF=dentate fascia, CA=hippocampal subfields, Cbl=cerebellar granular cell layer",yes
PMC6109099,Figure_3,oa_package/57/4c/PMC6109099.tar.gz,"[' 3a, b).', ' 3b and Supplementary 4A).', ' 3b).', 'Impaired wound healing and stress response in JunB cKO mice.', 'Scale bars, 50 mSimilarly, micro-injuries due to hair plucking significantly induced hyperplasia of the epidermis and sebaceous glands in JunB cKO (', ' 3c).', ' 3c).', ' 3d), thus confirming its anti-proliferative, possibly tumor suppressing effects.', ' 3c).']","Fig. 3 Impaired wound healing and stress response in JunB cKO mice. JunB cKO mice display delayed wound healing compared to wild type mice following 6mm full-thickness circular biopsy punch excision. ** <0.001, one-way ANOVA ( =7) Representative H&E photomicrographs of a 10 days wound depicting a hyperplastic and thickened epidermis and enlarged sebaceous glands in JunB cKO mice compared to wild type mice. Scale bars, 50m. Enhanced epidermal and sebaceous gland hyperplasia in JunB cKOs at day 5 following hair plucking. Inset image depicts opposing role of JunB (red) on proliferating cells marked in green with Ki67. Quantification of the size of sebaceous glands following hair plucking, *** <0.001, -test ( =3). The topical application of TPA, a strong enhancer of proliferation, for 7 days markedly triggered epidermal and sebaceous gland hyperplasia. Quantification of the size of sebaceous glands after TPA application, *** <0.001, t-test ( =3). E, epidermis; D, dermis; HF, hair follicle; SG, sebaceous gland; SC, subcutaneous layer; PC, panniculus carnosus. Scale bars, 50m",yes
PMC5089525,Figure_1,oa_package/87/ed/PMC5089525.tar.gz,"['In ADNI, plasma tau was higher in patients with AD dementia (figure 1A) compared with CNs and patients with MCI.', ""Plasma tau, diagnosis, and CSF -amyloid-42 (A 42)Baseline data from the Alzheimer's Disease Neuroimaging Initiative (ADNI)."", 'High plasma tau correlated weakly with low CSF A 42 in ADNI (figure 1B, table 2).', 'Patients with AD dementia had higher plasma tau than all other groups, and A -positive patients with MCI had higher plasma tau than A -negative patients with MCI (figure 1C).']","Figure 1 Plasma tau, diagnosis, and CSF -amyloid-42 (A ) Baseline data from the Alzheimer's Disease Neuroimaging Initiative (ADNI). (A) Plasma tau and diagnosis. Values from a linear regression model with plasma tau as the dependent variable and diagnosis as a categorical predictor, adjusted for 4, age, and sex. Cognitively healthy controls (CNs) had lower levels than patients with Alzheimer disease (AD; = 0.48, 95% confidence interval [CI] 0.19 to 0.78), and patients with mild cognitive impairment (MCI) had lower levels than patients with AD ( = 0.38, 95% CI 0.65 to 0.098). (B) Plasma tau and CSF A . The trend line is from a linear regression model with plasma tau as the dependent variable and CSF A as the predictor, adjusted for diagnosis, 4, age, and sex. The shaded area indicates the 95% CI. In a sensitivity analysis, we removed the 2 bottom-right observations. This affected the slope, but plasma tau and CSF A were still correlated ( = 3.99, 95% CI 7.68 to 0.30, = 0.034 compared to = 4.86, 95% CI 8.38 to 1.33, = 0.0071 with all observations included). The coefficients are on the original scale of CSF A ; see for coefficients using standardized data. (C) Plasma tau in diagnostic groups stratified by CSF A (A-positive, CSF A <192 ng/L, closed circles; A-negative, CSF A >192 ng/L, open circles). Values from a linear regression model testing the effect of the combination of diagnostic group and A status to predict plasma tau, adjusted for 4, age, and sex. Patients with AD had higher plasma tau levels than A-negative CNs ( = 0.62, 95% CI 1.070.17), A-positive CNs ( = 0.67, 95% CI 1.160.18), A-negative patients with MCI ( = 0.90, 95% CI 1.37 0.42), and A-positive patients with MCI ( = 0.43, 95% CI 0.780.081), and A-positive patients with MCI had higher plasma tau levels than A-negative patients with MCI ( = 0.47, 95% CI 0.900.031).",yes
PMC8602824,Figure_4,oa_package/46/51/PMC8602824.tar.gz,[],"Figure4 The responses of serum immunoglobulin after rAlt a 1 treatment. T-IgE levels in serum after challenges. Serum -sIgE, -sIgG1, -sIgG2a, and -sIgG2b levels. The expressions of rAlt a 1-sIgE, rAlt a 1-sIgG1, rAlt a 1-sIgG2a, and rAlt a 1-sIgG2b. The levels of neutralizing antibodies in serum (n = 6 per group). Values are means SEMs. * < .05, ** < .01 compared to the PC group. NC, negative group; PC, positive group; 5 g, 50 g, 100 g, 150 g: 5 g, 50 g, 100 g, 150 g rAlt a 1 SCIT group.",yes
PMC10899128,Figure_3,oa_package/34/4f/PMC10899128.tar.gz,"[' 3 illustrates the difference between [18F]-FDG PET/MRI and other traditional imaging methods for GTV delineation.', 'Sixty nine-year-old female with nasopharynx cancers.', 'e The yellow line represents GTV-PETIn addition to the abovementioned effects of PET/MRI on the target area, manual delineation of tumor volume introduces considerable potential for inter-observer variability [63], which can be mitigated through the application of standardized segmentation techniques.']",Fig. 3 Sixty nine-year-old female with nasopharynx cancers. The blue line represents gross tumor volume (GTV)-PET/CT. The pink line represents GTV-PET/MRI. The red line represents GTV-CT. The green line represents GTV-MRI. The yellow line represents GTV-PET,yes
PMC10511090,Figure_4,oa_package/46/36/PMC10511090.tar.gz,"['In situ hybridization (ISH) results in the lymph nodes for JUNV and GTOV infected animals were similar in severity, spatial and temporal distribution across days (A and 4B).', 'g004Temporal severity of ISH/viral RNA labeling in select organs for both JUNV and GTOV groups.', 'In situ hybridization findings in the thymus includes viral RNA labeling at days 7, 10 and 12, increasing in severity over time, and following a similar temporal pattern across JUNV and GTOV infected animals with some slight variations in severity (D).', 'In situ hybridization findings in the bone marrow included viral RNA labeling of the day 7, 10 and 12 animals for both JUNV and GTOV (E).', 'Labeling was seen in a similar temporal pattern between JUNV and GTOV infected animals, but day 12 animals in the JUNV group had consistently higher severity scores (G).', 'Viral RNA labeling in the lung is discussed above in reference to specific lesions, but was also noted in other areas throughout the lung and ranged in severity from minimal to marked (H).', 'Severity increased over time with marked labeling present in day 12 JUNV infected animals (H).', 'Temporal labeling was similar and increased over time for both JUNV and GTOV infected animals with the day 12 JUNV infected animals receiving a slightly higher severity score (H).', 'g010"" position=""float""/>Viral RNA labeling was seen on days 10 and 12 in the esophagus and stomach and in days 7, 10 and 12 in the esophagus, stomach and small intestine (I).', 'The labeling patterns (temporal and spatial) between JUNV- and GTOV-infected animals were similar but the severity scores in the JUNV day 12 animals were higher than those of the GTOV-infected animals (I).', 'The overall labeling pattern shows an increase over time from day 7 to 12 (I).']",10.1371/journal.pntd.0011620.g004,yes
PMC5342686,Figure_2,oa_package/05/6b/PMC5342686.tar.gz,['BEA inhibited PMA-induced up-regulation of inflammatory mediators of lymphocytesThe levels of TNF- A.'],"Figure 2 BEA inhibited PMA-induced up-regulation of inflammatory mediators of lymphocytes The levels of TNF- , IL-12 and IFN- released by lymphocytes under different treatments were examined using ELISA kits as described above. Data are presented as meanSD of triplicates. Statistical analysis between PMA group and CsA or BEA treatment groups was determined by student's t-test. Significance level was labeled as following: ** <0.01; *** <0.001.",yes
PMC8692797,Figure_8,oa_package/5e/59/PMC8692797.tar.gz,"['EGF, HGF, IGF-BP2, IGF-BP6, and FGF2 were detected in the dot blot of the KORSCM (A).', 'In the HHFDPC and KORS extracts, HGF (full length) and VEGF were detected (B).', 'Protein array analysis of HDMEC extracts revealed the presence of VEGFR2, c-Met, and FGFR2 receptors and the presence of FGF2 and PDGF-BB GFs (C).', '(E) A reverse immunoprecipitation assay, corresponding to the co-immunoprecipitation shown in D, was conducted from HDMEC whole cell protein extracts using anti-VEGFR2 or c-Met antibody.', 'After GPC1 precipitation, c-Met, the c-MET precursor form (pro-c-Met) and VEGFR2 were detected by immunoblotting (D).', 'Moreover, conversely, after precipitation with c-Met or VEGFR2 antibody, GPC1 was detected by immunoblotting (E).']","FIGURE 8 HDMEC express the receptors for VEGF and HGF, which are secreted by KORS. Growth factors secreted by KORS analyzed by protein array from their conditioned medium (KORS ) and compared to the basal cell culture medium (EC ) without cells. Green frames: positive control spots; red frames: negative control spots; black frames: spots corresponding to the indicated target. The production of HGF and VEGF in the KORS were compared to that of the HHFDPCs by Western immunoblotting. Growth factors and receptors of HDMECs analyzed by protein array. Green frames: positive control spots; red frames: negative control spots; black frames: spots corresponding to the indicated target. The direct interaction between GPC1 with VEGFR2 or c-Met was analyzed by co-immunoprecipitation. A whole cell protein extract (25g) from the HDMECs was precipitated using anti-GPC1 antibody. Anti-c-Met or anti-VEGFR2 antibody was used to reveal the membrane. A control experiment without antibody was performed. A reverse immunoprecipitation assay, corresponding to the co-immunoprecipitation shown in , was conducted from HDMEC whole cell protein extracts using anti-VEGFR2 or c-Met antibody. Then, the isolated immunocomplexes were immunoblotted using anti-GPC1 antibody.",yes
PMC10663703,Figure_3,oa_package/b9/8f/PMC10663703.tar.gz,"['The top markers of C2-HPGD, C5-AHCY, C6-HPN, C9-FTX, C10-KLK12 and C12-TPPP3 were highly expressed in tumorous prostates (A).', 'While the gene expression characteristics of C1-MT1M, C3-KRT15, C4-GADD45B, C7-SCD, C8-MGP and C11-TGM4 were more similar to normal tissues (A).', 'Consistently, abundant upregulated genes in PCa tissues were also detected in C2-HPGD, C5-AHCY, C6-HPN, C9-FTX, C10-KLK12 and C12-TPPP3 (B).', 'Identification of malignant cells by clinical correlation analysis of each subpopulation in PCa progression.', 'To further validate the clinical correlation of each subpopulation, we examined the expression levels of PCa-upregulated genes in each subpopulation (Table S3) and found that these genes were mostly expressed in the potentially malignant subpopulations we identified earlier (', 'Despite moderate expression of PCa-upregulated genes in cells from normal samples was detected, the positive cell counts were small and the expression levels were relatively low (C, D).']",Fig. 2 Epithelial cell reclustering and the characterization of each subpopulation. (A) Epithelial cells reclustered into 12 subpopulations. (B) The expression levels of representative markers of each epithelial subpopulation. (C) Normal and tumorous tissue distribution of each subpopulation. (D) Cell proportion of each subpopulation in tumorous vs normal tissue. (E) The expression levels of PCa progression-related genes in each subpopulation. (F) The pathway enrichment analysis of each subpopulation evaluated by GSVA.,yes
PMC7102211,Figure_7,oa_package/65/5a/PMC7102211.tar.gz,"[' 7a e).', '', '01\nDiscussionThe Kunming mouse strain is widely used in China comprising about 70 % of the mice used in research, as it is easy to handle and produces large litter sizes.']","Fig.7 Cytokine profiling of the colon: Up-regulated expression of inflammatory cytokines in the colon of mice in response to the DSS challenge relative to the wt control. Colon extracted total RNA was used for RT-qPCR employing exonexon primers specific for murine , , , - , and - . was chosen as endogenous reference. Data is displayed as meanstandard deviation; * <0.05, ** <0.01",yes
PMC9289277,Figure_5,oa_package/e3/53/PMC9289277.tar.gz,"['The results demonstrated that the acne images were mainly shown in the form of fake cysts, hair follicles, fistulas, and calcifications (), which is in line with some previous studies.']","FIGURE 5 HFUS in identifying the different types of acne. A false cyst-type dermis and subcutaneous tissue within a low-echo nodule; the arrow indicates the rich blood flow around the nodule; The hair follicle type between the two arrows shows a slightly tilted low echo across the dermis; A fistula type low-echo structure of the belt between the two arrows is located in the dermis and subcutaneous tissue layer; The calcification arrow shows the dot calcification stove in the dermis. (d: dermis, st: subcutaneous tissue, m: face muscle).",yes
PMC4890852,Figure_1,oa_package/ec/06/PMC4890852.tar.gz,"['Data AvailabilityAll relevant data are within the paper and its Supporting Information files with the exemption of data for A 1C, which are available upon request via an online request tool listed in S2 Tables, and , the clinical diagnoses of dementia and AD were made following National Institute of Neurological and Communicative Disorders and Stroke Alzheimer s Disease and Related Disorders Association recommendations [GdCl3 treatment reduced TNBS-induced TNF , IL-1 and IL-6 expressions.']","Figure 3 GdCl treatment reduced TNBS-induced TNF, IL-1 and IL-6 expressions. After induction of TNBS colitis, GdCl was given to mice intravenously on day 3. And mice were killed on day 7 or day 14. The amount of mucosal macrophages in colon and the levels of cytokines in serum and colonic mucosa were evaluated. (A) Proportion of mucosal macrophages in colon. (B) Proportion of mucosal CD3 cells in colon. (C) Cytokine expression of TNF, IL-1 and IL-6 in serum. (D) Cytokine levels of TNF, IL-1 and IL-6 in colonic mucosa. (Ctrl, control; G, GdCl ) (ns: no significance; , ; , , comparing TNBS + GdCl to TNBS).",yes
PMC4121513,Figure_1,oa_package/4f/7f/PMC4121513.tar.gz,"['ResultsOptimization of an 8-mer ASO for in vivo applicationWe previously reported that an 8-mer 2 OMeP ASO (3UP8) that targets a GCRS that partially overlaps with ISS-N1 corrects SMN2 exon 7 splicing in SMA patient cells (c, lane 3).', '21 Another 8-mer ASO (F8) that targeted the first eight residues of ISS-N1 had no effect on SMN2 exon 7 splicing, suggesting the effect of 3UP8 is independent of ISS-N1 and specific to the GCRS (c, lane 2).', 'To circumvent this problem, we generated 3UP8i, an improved version of 3UP8 by incorporating PEG-282 and propyl modifications at the 5 and 3 ends, respectively (b).', '24,25 As expected, 3UP8i retained the GCRS-specific stimulatory effect on SMN2 exon 7 splicing in SMA patient cells (c, lane 5).', 'A control 8-mer ASO with similar modifications and a single-nucleotide substitution (CON8i) produced no appreciable effect on SMN2 exon 7 splicing (c, lane 4).', 'In vivo, 3UP8i (20 g/g body weight) showed noticeable stimulation of SMN2 exon 7 splicing in liver (d, lane 4).', '3UP8i showed substantial stimulation of SMN2 exon 7 inclusion in the liver, whereas CON8i had no noticeable effect (d, lanes 5 10).', 'Effect of GC-rich sequence (GCRS)-targeting ASOs on splicing of SMN2 in vitro and in vivo.']","Figure 1 . ( ) Diagrammatic representation of annealing positions of ASOs targeting GCRS within intron 7. Negative regulators of exon 7 splicing, GCRS, ISS-N1 and hnRNP A1 binding sites are highlighted with boxes. Annealing positions of ASOs (3UP8, 3UP8i, CON8i, and F8) are shown as horizontal bars. Numbering of nucleotides starts from the first position of intron 7. ( ) Sequences of ASOs used in this study. All ASOs employed for mouse studies had PEG-282 and propyl modifications at the 5 and 3 ends, respectively. ( ) exon 7 inclusion in SMA patient fibroblasts after transfection with various ASOs. ( ) exon 7 inclusion in the liver of heterozygous mice ( ; ) after treatment with various ASOs. Each ASO was administered by intraperitoneal (IP) or subcutaneous (SC) route at the indicated dose. Exon 7-included and exon 7-skipped products are indicated as FL and 7, respectively.",yes
PMC3283855,Figure_1,oa_package/3c/21/PMC3283855.tar.gz,"['The lesion also had central ulceration covered by a scant, cheesy, and purulent discharge ().', 'Implications for a therapeutic test of diagnosisInt J Dermatol20054412112415689209Erythematous suppurative plaques with central ulceration on the surface of her neck.']",Fig. 1 Erythematous suppurative plaques with central ulceration on the surface of her neck.,yes
PMC10402180,Figure_1,oa_package/7d/6d/PMC10402180.tar.gz,"['The 72 kDa protein is composed of a catalytic kinase domain, a flexible interdomain region B (IB), and two tandem SH2 (SYK-tSH2) domains connected by interdomain A (IA) (A) (1).', 'Generation of the doubly phosphorylated ITAM (p-ITAM) is initiated by the stimulation of receptor complexes with IgE, IgG or IgM, which triggers the phosphorylation of ITAM motifs by Lyn or Fyn Src kinases (B) (4).', '34445310.']","Figure 1. SYK topology and activation mechanism with Fc receptors. Domain representation of SYK. (N)SH2 domain (residues 8118), interdomain A (IA), (C)SH2 domain (residues 163264), interdomain B (IB), and the catalytic kinase domain (364620). The activation mechanisms of autoinhibited SYK via both autophosphorylation and engaging p-ITAM on Fc receptors. Tyrosines phosphorylated via these mechanisms are highlighted.",yes
PMC6188173,Figure_7,oa_package/5f/18/PMC6188173.tar.gz,['Hematoxylin and eosin (H E) stain of lesion.'],"Figure 7 Hematoxylin and eosin (H&E) stain of lesion. The lesion is a spindle cell neoplasm infiltrating in a highly collagenous background. H&Estain, 100x.",yes
PMC3762856,Figure_2,oa_package/a5/ff/PMC3762856.tar.gz,"['The increase of body weight was suppressed during exercising in APP-HFD+Ex 0 10 mice and APP-HFD+Ex 5 15 mice, although it was not as evident as that in APP-HFD+Ex 10 20 mice (A).', 'Fasting glucose levels in APP-HFD+Ex 0 10 mice and APP-HFD+Ex 5 15 mice were not different from those in APP-HFD mice, which were higher than that in APP-HFD+Ex 10 20 mice (B.', 'Glucose tolerance ability was clearly deteriorated in APP-HFD+Ex 0 10 mice and APP-HFD+Ex 5 15 mice compared with that in APP-HFD+Ex 10 20 mice (B).', 'Insulin levels were not different among APP-HFD mice, APP-HFD+Ex 0 10 mice, APP-HFD+Ex 5 15 mice and APP-HFD+Ex 10 20 mice (C).', 'Importantly, running distances in APP-HFD+Ex 0 10 mice and APP-HFD+Ex 5 15 mice were longer than those in APP-HFD+Ex 10 20 mice (D), indicating that the deterioration of glucose metabolism in APP-HFD+Ex 0 10 mice and APP-HFD+Ex 5 15 mice was not caused by reduction of physical activity.', 'As in APP transgenic mice, HFD after finishing exercise increased body weight (E) and reduced glucose tolerance (F and G) in WT-HFD+Ex 0 10 mice and WT-HFD+Ex 5 15 mice.', 'g002HFD after finishing exercise deteriorated glucose tolerance in APP-HFD mice.', 'Actually, obesity and glucose intolerance were clearly observed in WT-HFD+Ex 0 10 and WT-HFD+Ex 5 15 mice (), indicating that HFD after finishing exercise disrupted metabolic conditions, which might also damage neuronal functions in these mice.']",10.1371/journal.pone.0072796.g002,yes
PMC8402728,Figure_1,oa_package/03/d2/PMC8402728.tar.gz,"['Hemocyte protein was isolated and analyzed by anti-His western blot, which verified recombinant protein expression in hemocyte samples (A).', 'Anti-His (VnxG-His, VnxQ2-His; B) and anti-FLAG (FLAG-Mc4r; C) immunomicroscopy results corresponded to patterns observed Sf9 cells infected with the recombinant viruses [21], and Sf9 and High Five cells transfected with vinnexin expression plasmids [30].', 'As a high percentage of hemocytes of larvae injected with the three viruses were GP64 positive (B,C), indicating hemocytes were infected with the viruses, we tested whether infection likewise altered hemocyte number in vivo.', 'We observed cellular localization () reminiscent of VnxQ2 in CsIV-infected caterpillars [18], as well as VnxG and VnxQ2 expression in cell culture [21,26,30].', 'CRC PressBoca Raton, FL, USA1991133167Vinnexins are expressed by recombinant baculoviruses in larval H.']","Figure 1 are expressed by recombinant baculoviruses in larval hemocytes. Newly molted 4th instar larvae were injected with 100 pfu recombinant baculoviruses or media and hemocytes isolated 3 dpi. ( ) Expression of His-tagged was verified with anti-His western blot. Mock: mock treated; Mc4r: AcMNPV-FLAG- c4r; VnxG: AcMNPV- -His; VnxQ2: AcMNPV- -His. Bottom panel is anti-tubulin as loading control. ( ) Anti-His and anti-GP64 immunomicroscopy was performed to verify Vinnexin expression and AcMNPV infection, respectively. ( ) Anti-FLAG and Anti-GP64 immunomicroscopy was performed to verify FLAG-Mc4r expression and AcMNPV infection.",yes
PMC5977763,Figure_2,oa_package/ec/c3/PMC5977763.tar.gz,"['c\nneb / mutants exhibit F-actin (red) and Actinin2 (green) positive aggregates at the myosepta (arrowheads) (and zoomed inset) compared to wildtype siblings at 2 dpfResultsneb mutants display a reduction in muscle functionWe obtained a zebrafish mutant strain (sa906) which contains a point mutation in neb causing a nonsense mutation in exon 30 (of 134) of the transcript.', ' 2a).', '2b).', '2c).', '2c inset).']","Fig. 2 Characterisation of skeletal muscle pathology in fish. Gomori trichome staining of skeletal muscle sections reveal the presence of dark regions (arrows) throughout the muscle indicative of nemaline bodies not observed in fish. Nuclei (arrowhead) are evenly organized in , however, appear disorganized in fish. Quantification of normalized fiber area from Gomori trichome stained sections in ( =23 fibers) compared to fish ( =21 fibers). Error bars represent meanSD, *** <0.001. mutants exhibit F-actin (red) and Actinin2 (green) positive aggregates at the myosepta (arrowheads) (and zoomed inset) compared to wildtype siblings at 2 dpf",yes
PMC6532138,Figure_4,oa_package/0b/b6/PMC6532138.tar.gz,[':Intraoperative view of the cyst with a purulent orifice.'],Figure 4: Intraoperative view of the cyst with a purulent orifice.,yes
PMC9234383,Figure_4,oa_package/84/c8/PMC9234383.tar.gz,"['37-year-old female patient: (a) stomach-located bone fragment (arrow) and its extension to SMV, (b) thrombus appearance in SMV (arrow) two weeks after foreign body removalSMV: superior mesenteric veinForeign body localization and perforation were at the level of the ileum in all cases with perforation.']","Figure 4 37-year-old female patient: (a) stomach-located bone fragment (arrow) and its extension to SMV, (b) thrombus appearance in SMV (arrow) two weeks after foreign body removal SMV:superior mesenteric vein",yes
PMC9552083,Figure_2,oa_package/1b/4b/PMC9552083.tar.gz,[],Figure2 Hand-crafted feature extraction: texture and shape feature extraction of the cell nuclei; cavity feature extraction; feature extraction; color feature extraction.,yes
PMC8577461,Figure_6,oa_package/95/11/PMC8577461.tar.gz,"['4DiscussionIn this study, we identified a novel role for immunoproteasome in the regulation of -Syn aggregation and neurodegeneration in PD models ().', 'A schematic diagram underlying the role of immunoproteasome assembly disturbance in -Syn pathology and neurodegeneration in PD models.', 'Various neurodegenerative diseases including PD share a common pathological feature, that is, intracellular protein aggregation such as -Syn [28,29].']","Fig. 5 Dopaminergic neurons-specific overexpression of NRF2-POMP axis alleviates -Syn pathology. promoter contains NRF2 binding region (TGCTGTGTCAC) and ARE sequence (TGAGCGGCG). Dual-luciferase reporter assay was applied to detect the interaction between POMP promoter and NRF2. Western blot was applied to detect the nigral protein levels of 1i, 5i, PLK2, pS129 -Syn in 12-month A53T -Syn transgenic mice with dopaminergic neurons overexpressing NRF2 or POMP. The soluble and insoluble -Syn levels were detected in the SN of 12-month A53T -Syn transgenic mice with dopaminergic neurons overexpressing NRF2 or POMP. -Syn aggregation was detected by ThT immunofluorescence in the SN of 12-month A53T -Syn transgenic mice with dopaminergic neurons overexpressing NRF2 or POMP. Western blot was applied to detect the nigral TH in 12-month A53T -Syn transgenic mice with dopaminergic neurons overexpressing NRF2 or POMP. Rotarod test was used to evaluate the motor ability in 12-month A53T transgenic mice with dopaminergic neurons-specific overexpression of NRF2-POMP axis. * <0.05, ** <0.01, *** <0.001.",yes
PMC4224021,Figure_2,oa_package/41/7d/PMC4224021.tar.gz,"[' -Synuclein pathology is apparent in the hippocampal CA2 subregion of PD patientsNext, -synuclein pathology was examined in the HC.']","Figure 2 A significant increase was present in -synuclein deposits in PD patients compared to the control and iLBD cases (*p<0.001 vs Ctr, iLBD), and a significant increase in CD68 positive amoeboid microglia was present in PD patients compared to control subjects and iLBD cases (*p<0.01 vs Ctr; *p<0.05 vs iLBD). The number of CD68 positive amoeboid microglia in iLBD cases was also significantly higher compared to control subjects ( p<0.01 vs Ctr). Data represent meanSEM.",yes
PMC7102776,Figure_1,oa_package/9f/16/PMC7102776.tar.gz,"['There were also mild streaking infiltrates in bilateral lower lung fields, especially the perihilar regions, while no pleural effusion was noted (s 1\nand 2\nA).', ' 1Chest X-rays at presentation showed a well-demarcated round opacity in the left lower lung field (arrowheads).', '3 In a recent large study involving 109 children with round pneumonia, the consolidations tended to be solitary, have well-defined borders, and were located posteriorly and in bilateral lower lobes (63%),4 which exactly matched the initial radiographic findings in our patient ( 1).']",Figure1 Chest X-rays at presentation showed a well-demarcated round opacity in the left lower lung field (arrowheads). Note the apparent air bronchogram on the posteroanterior view (arrow).,yes
PMC11197076,Figure_1,oa_package/d2/6f/PMC11197076.tar.gz,[],FIGURE 1 Two examples for comparison of ultrasound imaging in the lesions and uninvolved skin.,yes
PMC11288697,Figure_5,oa_package/89/9d/PMC11288697.tar.gz,"['Correlation AnalysisThe results are presented in s 5(d), 5(e), and 5(f).', '004"" position=""float""/>(a) Lung ultrasound score in each group.']",Figure 5 (a) Lung ultrasound score in each group. (b) Mean pulmonary pressure in each group. (c) Pathological score in each group. (d) Lung ultrasound score was positively correlated with mean pulmonary artery pressure (mPAP). (e) Pathological score was positively correlated with mPAP. (f) Lung ultrasound scores were positively correlated with pathological score.,yes
PMC2988898,Figure_1,oa_package/b7/ee/PMC2988898.tar.gz,"[""Capsule endoscopy found segmental distribution of ulceration, erosion and nodal hyperplasia in the proximate jejunum consistent with Crohn's disease (fig. 1a)."", 'When the scope passed 20 cm from the Treitz ligament we found jejunum erosion, nodal hyperplasia, stenosis (fig. 1b) and a capsule there.', 'We grasped the capsule with a snare and pulled it out (fig. 1c).', 'The next day, barium examination revealed that the stenosis was near to the Treitz ligament (fig. 1d).', 'References1FarhatMHShamseddineAIBaradaKASmall bowel tumors: clinical presentation, prognosis, and outcome in 33 patients in a tertiary care centerJ Oncol20082008212067192660872ModlinIMGustafssonBIDrozdovIPrincipal component analysis, hierarchical clustering, and decision tree assessment of plasma mRNA and hormone levels as an early detection strategy for small intestinal neuroendocrine (carcinoid) tumorsAnn Surg Oncol200916487498190509633CaveDLegnaniPde FranchisRICCE consensus for capsule retentionEndoscopy20053710651067161897924LiFGuruduSRDe PetrisGRetention of the capsule endoscope: a single-center experience of 1,000 capsule endoscopy proceduresGastrointest Endosc20086817418018513723a Erosion and bleeding seen in the capsule endoscopy image.']","Fig. 1 Erosion and bleeding seen in the capsule endoscopy image. The jejunum erosion, nodal hyperplasia and stenosis seen in the DBE image. Capsule endoscope retained at the jejunum stenosis and pulled out with a snare. Jejunum stenosis as seen in abdominal barium meal X-ray film.",yes
PMC10577053,Figure_2,oa_package/c5/4d/PMC10577053.tar.gz,"[' 1 below) into which a percutaneous drain was placed under ultrasound guidance (see ', ' 1']",Fig.2 Axial non-contrast CT abdomen after treatment showing post-surgical changes of cholecystectomy and right upper quadrant abscess pigtail drain placement (blue arrow) with interval decreased size of the previously seen right upper quadrant abscess which closely abuts the hepatic flexure of the colon (yellow arrow).,yes
PMC8665644,Figure_3,oa_package/5f/e9/PMC8665644.tar.gz,"[' 3A F).', '3G I), which was opposite to the 18F-BCPP-EF PET finding.', ' 3J L).', 'Double immunostaining for ATPB (green) and CaMKII (red) in the cerebral cortex of 15-week-old SAMR1 mice (A C) and 5-week-old (D F), 15-week-old (G I) and 25-week-old (J L) SAMP10 mice.', 'n = 4 for each group, except for 25-week-old SAMP10 mice (n = 2)As ATPB immuno-positive signals were also observed in non-neuronal cells, as shown in ', ' 3G, we next analyzed the localization of ATPB in astrocytes.', ' 3, 4 and 5 show that in 15-week-old SAMP10 mice, mitochondrial ATPB, a key enzyme for ATP production, was mainly present in neurons, although some was present in microglia, and slightly elevated levels were also present in reactive/perivascular astrocytes.']","Fig. 3 Double immunostaining for ATPB (green) and CaMKII (red) in the cerebral cortex of 15-week-old SAMR1 mice ( ) and 5-week-old ( ), 15-week-old ( ) and 25-week-old ( ) SAMP10 mice. Quantification of the immunofluorescent signals. Note that the ATPB signal had greatly increased and was localized to the neurons at 15weeks of age, but dramatically decreased at 25-week-old. Arrowheads indicate ATPB signals. Scale bar: 10m. Data are meansSEs. They were analyzed by one-way ANOVA followed by the Bonferroni test (** <0.01). =4 for each group, except for 25-week-old SAMP10 mice ( =2)",yes
PMC11617428,Figure_1,oa_package/52/8d/PMC11617428.tar.gz,"['Radiographic examination revealed dens invaginatus type II,11 a wide root canal, mid-root horizontal fracture, and a large radiolucency between the fragments and signs of inflammatory root resorption ().', 'Diagnostic radiograph dens invaginatus* class I can be observed extending to the coronal third of the root.']",Fig. 1 Diagnostic radiographdens invaginatus* class I can be observed extending to the coronal third of the root. Notice the wide root canal with signs of inflammatory root resorption on the distal aspect of the root. A horizontal root fracture separates the apical third of the root from the coronal part,yes
PMC7695736,Figure_1,oa_package/5c/d7/PMC7695736.tar.gz,"[' 1 shows a large metastatic lymph node that can be observed in post-contrast DCE-MR, FDG-PET, and DW images.', ' 1C, E) after the image co-registration and using the same ROI for data analysis of the three modalities.', ' 1 also shows the mean data from a metastatic lesion ROI for three modalities, which demonstrates that the signal models used in these modalities are appropriate.', ' 1F for both the fast wash-in and wash-out phases (Fp = 1.', ' 1G), the log signal decreases linearly within the range of b-values used in this study (ADC = 0.', ' 1H) shows an initial vascular phase with a sharp peak followed by a slow irreversible uptake of tracer (K1 = 1.', 'An example of a 50-year-old male patient with HNSCC in the floor of the mouth.', 'Nodal size and SUVConventional clinical measures, nodal size and specific uptake value (SUV), are shown in ']","Figure 1 An example of a 50-year-old male patient with HNSCC in the floor of the mouth. A left level-1 metastatic lymph node can be detected in co-registered DCE-MRI (SimpleElastix v0.10.0; ) ( ), co-registered 18F-FDG-PET image ( ), PET activity map overlaid on DCE-MRI ( ), co-registered DWI b0 image ( ) and DWI b0 image overlaid on DCE-MRI ( ). The average data (blue dots) of the left level 1 metastatic lymph node (arrows) are shown for DCE-MRI ( ), DWI ( ), and PET ( ) with their corresponding model fits (solid black lines).",yes
PMC8091422,Figure_7,oa_package/3c/8e/PMC8091422.tar.gz,"['1177_0192623320970534-fig7""/>Staining for a given IHC procedure typically was comparable for a given tissue for all 3 immunostainers when visually evaluating stained slides (Supplemental Table 7) and interpreting measurements of positive cell counts obtained by DIA (s 5A and 8A).', 'The reduction in staining intensity for the Autostainer Link 48 depended on the biomarker and tissue ( and Supplemental Table 7).', 'Such modest discrepancies will not impact interpretation of DIA data if the end point being examined ties staining to a particular structure (eg, labeled cells), whereas interpretation based only on the cumulative staining intensity (ie, positive pixels, wherever they occur in the section) may be impacted if 1 instrument consistently delivers a lighter stain (s 7 and 8).']","Figure 7. Precision (repeatability and reproducibility) of IHC staining for OD-based assessments generally is comparable across stainers, although subtle variations (eg, reduced average OD values for instrument 0151 for several biomarkers in colon, lymph node, and spleen) may be detected. These OD differences do not impact analysis of IHC data where the final interpretation is based on labeling of a specific tissue feature (eg, counts of positive cells; see ). IHC indicates immunohistochemical; OD, optical density.",yes
PMC5626538,Figure_6,oa_package/e6/85/PMC5626538.tar.gz,"['(2016) to robustly detect the endogenous mouse PSEN2 protein and observed that it colocalized with LAMP1 in both WT and JIP3 KO neuronal cell bodies (, A and B).', 'Interestingly, we also noted that PSEN2 puncta additionally colocalized with LAMP1 in both WT axons and dendrites (, A and B).', 'Most importantly, we detected a striking buildup of PSEN2 in the LAMP1-positive axonal swellings of JIP3 KO neurons (, B and D).', '.', 'As the localization of PSEN2 at amyloid plaques has not been previously established, we next examined PSEN2 localization in the mouse 5xFAD model of AD (Oakley et al.', 'In this study, we have recapitulated key aspects of this phenotype using JIP3 KO neurons ( F).', 'Consistent with this prediction, ELISA assays revealed significantly elevated A 42 levels in JIP3 KO neurons ( E).']","Figure 6. (A and B) PSEN2, LAMP1, and MAP2 immunofluorescence shows that PSEN2 colocalizes with lysosomes in neuronal cell bodies, axons, and dendrites of both WT and KO neurons. Large accumulations of PSEN2 on lysosomes in axonal swellings are selectively observed in JIP3 KO neurons (arrows; images are representative of results from five independent cultures). Bars, 10 m. (C and D) Magnified images showing PSEN2 (green) and LAMP1 (red) colocalization in both WT (C) and JIP3 KO (D) axons. Bars, 1 m. All results obtained from DIV 1012 neuronal cultures. (E) Quantification of endogenous mouse A42 based on ELISA measurements (mean SD) from = 5 independent WT and KO cultures (***, P < 0.001, unpaired test). (F) Schematic diagram summarizing that axonal lysosome accumulations in the JIP3 KO neurons are accompanied by the local buildup of APP, BACE1, and PSEN2 and increased production of A42.",yes
PMC5646146,Figure_2,oa_package/03/95/PMC5646146.tar.gz,"[' 2a and c).', ' 2b and d).', ' 2a and b).', 'Temporal overexpression of Murf1 and accumulation of Murf1-positive nuclei in muscle 2 days post-burn.', '001\nThermal injury results in a transient recruitment of neutrophils, but not macrophages, into muscleTo investigate the local inflammatory effect of severe cutaneous burn on muscle, we looked for the presence of inflammatory cells in the gastrocnemius muscle.']","Fig. 2 Temporal overexpression of Murf1 and accumulation of Murf1-positive nuclei in muscle 2days post-burn. Representative Western blot for Murf1. Representative images of Murf1 immunohistochemistry. Images were obtained at 20 magnification. Quantification of Murf1 protein expression. Quantification of Murf1-positive nuclei in sham, 2days, 7days, and 14days post-burn. indicate Murf1-positive nuclei and indicate Murf1-negative nuclei. * <0.05, *** <0.001",yes
PMC10689245,Figure_18,oa_package/af/21/PMC10689245.tar.gz,[],"Extended Data Fig. 10 PCA clustering of selected hippocampal cell clusters as well as astrocyte and microglia subclusters against all measured pathologies. Principal component analysis plot for hippocampal clusters 1, 6, 7, 9, 28, astrocyte subclusters 3, 5, 7, and microglia subclusters 2, 8, 11 against all measured pathologies (hippocampal volume and coverage areas of AT8, Iba1, CD68, GFAP, and S100). PC1 and PC2 showed the overall relationship between clusters based on similarity of the estimated log odds ratio per unit change in six pathologies per cluster/subcluster. AS, astrocyte; MG, microglia. For details of the analyses, see Methods.",yes
PMC7855846,Figure_5,oa_package/75/65/PMC7855846.tar.gz,['Dif NPs decrease Staphylococcus aureus induced bone destruction during osteomyelitis.'],"Figure 5 DifNPs decrease induced bone destruction during osteomyelitis. (A) Representative IVIS images of mice 14 days postinfection following daily tail vein injections of either DifNPs ( =5) or BlankNPs ( =5). Blue circles denote ROIs for quantitative analyses. (B) Fluorescence of infected and contralateral limbs in both groups of mice were assessed using ROI analysis of the limbs. Filled circles represent mice treated with BlankNPs, and open circles represent mice treated with DifNPs. Fluorescence intensity was normalized to the intensity of the corresponding ROI of phosphatebuffered saline (PBS)injected animals. Error bars represent mean . **** <.0001 as determined by Student's test. (C) Quantification of dissected organs ex vivo 14 days postinfection following daily tail vein injections of DifNPs or BlankNPs (same groups of mice as in A). Filled circles represent organs of mice treated with BlankNPs, and open circles represent organs of mice treated with DifNPs. Error bars represent mean . ** <.01 and **** <.0001 as determined by oneway analysis of variance (ANOVA). (D) A representative image of the analyzed dissected organs of 5C is shown: livers, spleens, kidneys, infected (Inf) femurs, and contralateral (Con) femurs. Organs of a PBSinjected mouse and a DifNPinjected mouse are shown with ROIs. The organs of the PBSinjected animal do not show fluorescence intensity above the image threshold. (E) Quantification of cortical bone destruction 14 days postinfection with and following daily treatment with PBS, BlankNPs, DifNPs, or freedrug diflunisal via tail vein injection. =921 mice per group. One mouse in the BlankNPs group experienced more than 20% weight loss and was euthanized. Different symbols (circles, triangles, and squares) represent three independent trials that included the groups as indicated by the corresponding symbols. Effect size (Hedges g) between BlankNP and DifNP groups=1.500 (95% confidence interval: 0.684, 2.317). The median femur from each group is shown in a threedimensional reconstruction to the right of the graph. Error bars represent mean . ** <.01 and **** <.0001 as determined by oneway ANOVA. (F) Quantification of bacterial burden by colonyforming units enumeration 7 days postinfection following daily treatment with PBS, BlankNPs, or DifNPs. =5 mice per group. One mouse in the PBS group was euthanized following an adverse response to anesthesia. Error bars represent mean . ns denotes no significance as determined by oneway ANOVA. (G) Representative histology images of femurs harvested from mice treated with BlankNPs or DifNPs and stained with a modified hematoxylin and eosin stain. Scale bars are as shown in the lower right corner of images",yes
PMC9477696,Figure_2,oa_package/e3/71/PMC9477696.tar.gz,['\nPathological findings.'],"Figure 2 A: Fine needle biopsy of the liver showed liver tissue and tumor tissue (low magnification of HE staining); B: The tumor cells are medium-large, consistent in shape, with a starry sky pattern, liver tissue found in the left and upper right corners (HE staining 200 magnification); C: Tumor cells have little cytoplasm, with basophilic, fine granular nuclear staining; tissue cells phagocytize apoptotic debris and nuclear fragments; it can be seen that there is multiple apoptotic debris (six) with coarse particles (red arrow) (HE staining 400 magnification); D: CD20 Labeling of tumor cells was diffuse and strongly positive (Envision method 200 magnification); E: CD10 labeling of tumor cells was diffuse and strongly positive (Envision method 200 magnification); F: BCL6 Labeling of tumor cells was diffuse and strongly positive (Envision method 200 magnification); G: Tumor cells were positive for MYC labeling (70%) (Envision method 200 magnification); H: Ki-67 proliferation index of tumor cells > 95% (Envision method 200 magnification).",yes
PMC2924279,Figure_2,oa_package/00/eb/PMC2924279.tar.gz,['The positive immunohistochemical expression of BAD in breast carcinoma tissue.'],Figure 2 .,yes
PMC3263677,Figure_1,oa_package/f3/62/PMC3263677.tar.gz,"['In 201 patients (50%), standard BCS was performed (without removal of AM); in 151 patients (37%), BCS-AM was performed (), the remaining 53 patients (13%) underwent total mastectomy (TM) as their initial surgical management.', 'Shaving additional margins for excision of invasive and noninvasive carcinoma has already been shown to be effective in reducing re-excision rates and reducing margin positivity for early stage breast cancer [9, 11 15] Cao and colleagues [11] retrospectively examined 126 patients with either DCIS (n = 23) or IDC (n = 103), all of whom underwent BCS-AM surgery that was similar in methodology to our series ().', 'American Journal of Surgery200819645565581880906313PovoskiSPJimenezREWangWPXuRXStandardized and reproducible methodology for the comprehensive and systematic assessment of surgical resection margins during breast-conserving surgery for invasive breast cancerBMC Cancer20099, article 25414RizzoMIyengarRGabramSGAThe effects of additional tumor cavity sampling at the time of breast-conserving surgery on final margin status, volume of resection, and pathologist workloadAnnals of Surgical Oncology20101712282341963662515HustonTLPigalargaROsborneMPTousimisEThe influence of additional surgical margins on the total specimen volume excised and the reoperative rate after breast-conserving surgeryAmerican Journal of Surgery200619245095121697896216ViciniFAKestinLLGoldsteinNSBaglanKLPettingaJEMartinezAARelationship between excision volume, margin status, and tumor size with the development of local recurrence in patients with ductal carcinoma-in-situ treated with breast-conserving therapyJournal of Surgical Oncology20017642452541132051517HwangESSamliBTranKNRosenPPBorgenPIVan ZeeKJVolume of resection in patients treated with breast conservation for ductal carcinoma in situAnnals of Surgical Oncology199858757763986952418RakovitchEPignolJPHannaWSignificance of multifocality in ductal carcinoma in situ: outcomes of women treated with breast-conserving therapyJournal of Clinical Oncology200725355591559617984188 (a) Schematic for BCS-AM.']","Figure 1 (a) Schematic for BCS-AM. The segmental excision surgical specimen is removed using wire localization. Additional margins can then be re-excised from each of the six faces of the cavity (anterior, posterior, superior, inferior, medial, and lateral). (b) BCS-AM surgical specimen. The total tissue obtained from the BCS-AM procedure includes the original localized lesion (shown with wire placement), as well as one or more additional margins from the six surfaces.",yes
PMC3853772,Figure_4,oa_package/43/30/PMC3853772.tar.gz,['Differential protein expression obtained by mass spectrometric analysis of P and DS.'],"Figure 4 Four of the 31 proteins reported in table are chosen and comparative expression analysis is presented as Box plots. Box plots show median value, standard deviation (SD), 50%, minimum and maximum intensity values and were exported from Marker View software.",yes
PMC10683898,Figure_2,oa_package/e0/63/PMC10683898.tar.gz,"['jomfp_496_22-f002"">Intraoral photograph reveals smooth surfaced, reddish pink, well-defined swelling on the left lower molar region with respect to 37 protruding out of the extraction socketAn orthopantomogram [] revealed ill-defined radiolucency involving the left mandibular body region extending from 35 to 38 along with marked thickening and enlargement of left interdental canal and mental foramen.']","Figure 2 Intraoral photograph reveals smooth surfaced, reddish pink, well-defined swelling on the left lower molar region with respect to 37 protruding out of the extraction socket",yes
PMC10569691,Figure_1,oa_package/e6/4a/PMC10569691.tar.gz,"['ResultsThe EVOC system preserves the tumor microenvironmentTo validate the robustness of the EVOC technology, which allows to maintain thick sections of different cancer types in culture for 5 days (11), we examined the survival and cell dynamics of colorectal cancer, urothelial carcinoma and breast cancer (A).', 'All tissue types showed a mean 95% viability on day 5 when compared to the tissue sampled immediately after surgical removal on day 0 (B).', '92, among samples of all tissue types (n = 16) of breast cancer, urothelial carcinoma and colorectal cancer (C).', 'An ex vivo organ culture (EVOC) system for preservation of the cancer microenvironment.', 'Corresponding to their respective pathways, trametinib downregulated pERK, palbociclib lowered pRB and NT219 blocked pStat3 staining (D).']","Figure 1 An organ culture (EVOC) system for preservation of the cancer microenvironment. Colorectal cancer (CRC), transitional cell carcinoma (TCC) and breast cancer (BC) were cultured in an EVOC assay that preserves the tumor microenvironment. Representative images of the tissues are shown on Day 0 when the tissue was received and after 5days in culture. Hematoxylin and eosin (H&E) and Ki67 staining are shown. Viability and proliferation of the different cancer samples were compared between Day 0 and Day 5 ( =5 per cancer type). Cancer viability and Ki67% stain was assessed and quantified by a pathologist showing non-significant changes during the culture period ( =16). The capacity for signal transduction modification was assessed by adding specific pathway inhibitors and determining their effect after 24h. Trametinib, a pERK inhibitor, palbociclib, a CDK4/6 inhibitor and NT219, a pStat3/insulin receptor substrate (IRS) inhibitor were added to the culture and their respective pathway targets were stained by immunohistochemistry showing downregulation of activity in the culture system.",yes
PMC4217770,Figure_2,oa_package/8e/86/PMC4217770.tar.gz,"['Certain lymphocytes showed nuclear invagination with cerebriform nuclei ().', 'Histopathological examination of case 1 by hematoxylin and eosin staining.']","Figure 2 Histopathological examination of case 1 by hematoxylin and eosin staining. (A) Hyperkeratosis of the epidermis, parakeratosis, acanthosis and dermal edema are exhibited. Dense, band-like lymphocytic infiltration was observed and atypical lymphocytes were infiltrating into the epidermis (scale bar length = 4 mm). (B) Enlarged view of A (scale bar length = 1 mm). (C) Enlarged view of B (scale bar length = 500 m). The papillary layer of the corium showed clear edema. The red blood cells appeared to show extravasation. Scattered lymph cells infiltrated in the mid-dermis. Certain lymphocytes showed nuclear invagination with cerebriform nuclei.",yes
PMC11188498,Figure_3,oa_package/55/6d/PMC11188498.tar.gz,"[' 3, the biopsy result showed inflammation, Red blood cell (RBC) extravasation, vascular proliferation, Fibroblast, vascular proliferation, Granulation tissue, and new bone formations.', '\nHistopathologic findings of the jaw.', 'C: Inflammation, Red blood cell extravasation, and vascular proliferation, H E stain, x100\nThe patient s laboratory test results showed an average white blood cell count of 8300/ L and an increased platelet count of 866,000 on admission, followed by 425,000 and 520,000 on discharge.']","Fig. 3 Histopathologic findings of the jaw. : Fibroblast and vascular proliferation, Granulation tissue, and new bone formation, Hematoxylin and eosin stain (H&E), x250. : Fibroblast and vascular proliferation, Granulation tissue, and new bone formation, H&E, x100. C: Inflammation, Red blood cell extravasation, and vascular proliferation, H&E stain, x100",yes
PMC11408811,Figure_3,oa_package/54/68/PMC11408811.tar.gz,"[' 2, ', 'This leads to a thickened and poorly defined hypertrophic zone (', ' 2']","Fig.3 The photomicrograph (high power) shows an early lesion of rickets. The section is taken from the growth plate through the region of the epiphyseal plate. There is decreased deposition of inorganic minerals in the cartilaginous tissue, between the hypertrophic columns of chondrocytes (arrow). Additionally, there is beginning of disorganization of the columnar growth pattern of the chondrocytes(Image Courtesy: Image #21271, Public Health Image Library, CDC. ).",yes
PMC10066120,Figure_3,oa_package/03/2d/PMC10066120.tar.gz,"[' 3).', 'A 22-year-old woman presented with abdominal pain, fever, and jaundice.', 'Abbreviation: GB, gallbladderA 23-year-old woman presented with abdominal pain.', ' 3).']","Fig. 3 A 22-year-old woman presented with abdominal pain, fever, and jaundice. A 64mm multilocular cystic mass in the segment VI of the liver was seen on US (a, asterisk), contrast-enhanced CT (b, asterisk), and MRI (c, single shot fast spin echo axial image, asterisk, d. single shot fast spin echo coronal image, asterisk). Arrows indicated the tumoral prolapse to the bile duct. Reader 2 could tell the biliary prolapse on US, CT and MRI, but reader 1 could tell it on US and MRI. Biliary prolapse was better delineated by MRI than CT because, as well as liver tumors, it showed separation (compare arrows in b and c). Abbreviation: GB, gallbladder",yes
PMC3579986,Figure_2,oa_package/02/80/PMC3579986.tar.gz,"['There is calcification within it (black arrow)\nAxial CECT of the abdomen and pelvis in a 23-year-old male patient with DSRCT.', ' 2), this was defined as being at least double the size of the next largest soft tissue deposit.', ' 2).', ' 2).']","Fig. 2 Axial CECT of the abdomen in a 25-year-old man with DSRCT. There is a large heterogeneous, mixed solid and cystic mass in the left upper quadrant ( ). The soft tissue component enhances. There is calcification within it ( )",yes
PMC11268842,Figure_5,oa_package/1b/01/PMC11268842.tar.gz,"['We used reverse loop technique which makes the axis better ().', 'Fluoroscopic image of balloon dilatation of the mitral valve.']",Figure 5 Fluoroscopic image of balloon dilatation of the mitral valve.,yes
PMC9354287,Figure_1,oa_package/39/10/PMC9354287.tar.gz,"['1 and 2).', 'There were no abnormal nodules or space occupying in the lungs on the chest X-ray before first operation']",Fig. 1 There were no abnormal nodules or space occupying in the lungs on the chest X-ray before first operation,yes
PMC11633847,Figure_2,oa_package/17/9e/PMC11633847.tar.gz,['Pleural biopsy(A) H E (200 magnification) shows bands and clusters of small lymphocytes and mesothelial cell hyperplasia.'],"Figure 2 Pleural biopsy (A) H&E (200magnification) shows bands and clusters of small lymphocytes and mesothelial cell hyperplasia. (B) The small lymphocytes are positive for CD43. (C) The cells of interest are also positive for CD20 CD, cluster of differentiation; H&E, hematoxylin and eosin",yes
PMC3403363,Figure_6,oa_package/1e/42/PMC3403363.tar.gz,"['Additionally, evaluation of the left temporal lobe one week following graded-CCI also confirmed immunoblot data; an obvious decrease of PSA-NCAM labeling of the soma and apical dendrites () of what are most likely layer II semilunar cells of the olfactory cortex [33].', '005""/>PSA-NCAM staining in left temporal lobe one-week following graded-CCI.']","Figure 6 PSA-NCAM staining in left temporal lobe one-week following graded-CCI. Diaminobenzidine staining for PSA-NCAM in sham (a), moderate (b), and severe (c) injury animals one week after CCI demonstrates decreased staining of the soma (large arrows) and apical dendrites (small arrows) of neurons in the piriform cortex. Insert denotes approximate region of interest at approximately bregma 1.70mm. Images are 20(x) magnification; scale bar = 300 m.",yes
PMC4053640,Figure_2,oa_package/88/be/PMC4053640.tar.gz,[],"Supplementary Table1 The numbers of mice used throughout this study are displayed at 1.5, 3, and 10months of age. WT is also referred to as NTg.",yes
PMC10080549,Figure_4,oa_package/17/23/PMC10080549.tar.gz,"['This showed that FTDtau1 or FTDtau1+2 expression did not induce ISR activation or sensitize for tunicamycin (TM)-induced ISR activation in neurons at 4 and 8 weeks ( 4C and D; Supplementary S7C).', 'In contrast, ISR activation and increased sensitivity for TM-induced ISR activation was observed in astrocytes in co-culture with FTDtau1+2-expressing neurons at 8 weeks, but not at 4 weeks (E and F; Supplementary S7D).', 'Intraneuronal tau aggregation induces the ISR in astrocytes.', 'Finally, we investigated whether the cell non-autonomous induction of oxidative stress and the ISR in astrocytes requires direct contact with neurons.']","Figure 4 Intraneuronal tau aggregation induces the ISR in astrocytes. ( and ) Schematic representation of the timeline of the experiment ( ) and experimental set-up ( ). ( ) Human neuronastrocyte co-cultures were transduced with EGFP-tagged FTDtau or FTDtau and treated for 48h with 10g/ml TM or vehicle control (Ctrl). Immunostaining was performed for MAP2 and ATF4, and cell nuclei were visualized using DAPI. ISR activation was determined by quantifying nuclear ATF4 intensity in neuronal and astrocytic nuclei by high-content microscopy (see ) at 4 ( and ) and 8 ( and ) weeks. =3 independent experiments and =12 wells. Bar graphs show meanSEM and data points represent mean/well. The lowest and highest values per experiment were set to 0 and 1, respectively. ShapiroWilk normality testing followed by two-way ANOVA with Tukey's post-hoc analysis. *** <0.001, **** <0.0001. ns, not significant. Widefield images of nuclear ATF4 immunoreactivity at 8 weeks are shown in . lists the exact -values and total number of cells analysed.",yes
PMC7110585,Figure_17,oa_package/5c/83/PMC7110585.tar.gz,[],"Fig.17 An intraosseous catheter in place for fluid diuresis for a pet rat ( ). A 25-gauge needle was used for the catheter, which was taped into place and capped with an IV catheter cap.",yes
PMC3112200,Figure_1,oa_package/95/56/PMC3112200.tar.gz,"['0020501-Mucke1"" ref-type=""bibr"">[30], resulting in a marked increase in the active isoform of cathepsin B in the brain as compared to vehicle-injected transgenic mice (A; ANOVA P 0.', 'Measures of A -degrading proteases neprilysin and insulin-degrading enzyme, as well as -secretase which prevents A production, were not altered (A and Table 2), thus the PADK-mediated lysosomal modulation was produced in a selective manner.', 'g001The lysosomal modulator PADK selectively enhances cathepsin B levels in APPSwInd mice.', 'In immunocytochemistry images, intracellular cathepsin B was revealed as punctate staining (green) characteristic of lysosomal organelles in hippocampal CA1 pyramidal neurons (B), and the intensity of such immunostaining was enhanced after PADK treatment (', '0001; D).', 'On the other hand, the number of cathepsin B-positive organelles per pyramidal neuron (n = 62) was found to be unchanged in the view-fields (E), and the lysosome-associated membrane glycoprotein LAMP1 was also unaltered in blot samples from the different treatment groups (', 'Brain samples from APPSwInd and APP-PS1 mice treated with vehicle or PADK were assessed for A x-42 and the truncated A x-38 species using selective sandwich ELISA protocols (1).', 'g0111Lysosomal modulator treatment promotes truncation of the A 1-42 peptide.', 'In 2A, hippocampal samples from the two mouse models were analyzed by immunoblot for the presynaptic protein synapsin II and the postsynaptic glutamatergic marker GluA1.', 'PADK treatment significantly reduced the GluA1 deficit in the two mouse models, reaching levels comparable to those found in non-transgenic control mice (2B) (ANOVA: P 0.', 'g0122Lysosomal modulation is associated with preservation of synaptic markers in APPSwInd and APP-PS1 mice.', 'Although the APPSwInd mice used in the present study express low levels of A deposits, their synaptic decline was associated with significant deficits on the suspended rod test (3A) as well as on the exploratory habituation test (', '001) (3A).', 'A marked degree of disinhibition was also displayed by the vehicle-treated transgenics on the second day of open field exploration, but APPSwInd mice injected with PADK were completely devoid of this behavioral defect (3B).', 'Also corresponding with synaptic marker decline, the APP-PS1 mouse model of extensive A pathology expressed cognitive deficiency in the hippocampal-dependent task of spontaneous alternation behavior (3C), similar to episodic memory deficits reported previously B, lower panel).', 'Approximately equivalent amounts of drebrin A were detected in 10 g of the input fraction (B, lane 1) and 10% of the IP fraction (B, lane 5).', 'Immunoprecipitated drebrin A bound to the C-term antibody, and protein G-sepharose was resuspended in cleavage buffer and incubated with or without purified calpain (C, lane 3 and 4, respectively).', 'Incubation with calpain reduced the amount of full-length drebrin A and produced several degradation products (C, lane 3), indicating that calpain cleaves drebrin A directly in vitro.', 'Alternatively, the SDS-PAGE migration of proteins in purified samples might be slower than in crude samples as observed in the case of -actin (B).', 'Calpain degraded purified drebrin A directly and produced several degradation products with molecular weights and proteolytic patterns that were similar to those in NMDA-treated cultured neurons () and ischemic brain sections (C).']",10.1371/journal.ppat.1011847.g002,yes
PMC10444656,Figure_3,oa_package/48/72/PMC10444656.tar.gz,"[' 3).', 'Peritoneal and mesorectal fascia (MRF) involvement.', 'D Tumor below the anterior peritoneal reflection, involving the MRF anteriorlySummary of update 4Terms describing tumor morphology have been separated from terms describing tumor composition (such as the mucinous component) in the updated lexicon.']","Fig. 3 Peritoneal and mesorectal fascia (MRF) involvement. Tumor of the anterior wall above the anterior peritoneal reflection involving the peritoneum. Tumor above the anterior peritoneal reflection on the lateral wall extends anteriorly to involve the peritoneum and posteriorly to involve the MRF. Tumor at the level of the anterior peritoneal reflection extends posterior and laterally, involving the MRF only. Tumor below the anterior peritoneal reflection, involving the MRF anteriorly",yes
PMC3513847,Figure_3,oa_package/a5/d3/PMC3513847.tar.gz,"['Arterial supply was noted from the superficial temporal artery, middle meningeal artery, and branches of the occipital artery [].', 'Pre-embolization angiography showing tumor blush from the branches of the left external carotid artery (a), right middle meningeal artery (b), right internal carotid artery (c), and the right external carotid artery (d)Postoperative computed tomography head (3D) scans showing the extent of calvarial resectionPathologyMicroscopic sections of the tumor demonstrated variable cellularity and growth pattern [].']","Figure 3 Pre-embolization angiography showing tumor blush from the branches of the left external carotid artery (a), right middle meningeal artery (b), right internal carotid artery (c), and the right external carotid artery (d)",yes
PMC6534680,Figure_1,oa_package/95/c8/PMC6534680.tar.gz,"['This lesions arise from the dura of the petrosal surface of the temporal bone, lateral to the trigeminal nerve (\n\n).', 'Keywordscerebellopontine anglemeningiomaskull basetumor\n(\nA\n) Osseous demonstration of the region involved in the retrosigmoid approach; (\nB\n) identification of the lower cranial nerves; (\nC\n) preservation of the VII/VIII nerves complex, V nerves and Petrosal Vein (PV) after tumor resection.']","Fig. 1 ( ) Osseous demonstration of the region involved in the retrosigmoid approach; ( ) identification of the lower cranial nerves; ( ) preservation of the VII/VIII nerves complex, V nerves and Petrosal Vein (PV) after tumor resection. Tu, tumor.",yes
PMC4689401,Figure_6,oa_package/f0/76/PMC4689401.tar.gz,"['We found that MFIs of MAP2 immunoreactivity in the pyramidal neurons of CA1 and CA3 regions of the hippocampus were decreased in AAV1-I1\nPP2A rats compared with AAV1-GFP controls (A and 6B).', 'The expression of total tubulin, however, was not changed in AAV1-I1\nPP2A rats with or without memantine (C).', 'We found a significant Fluoro-Jade B staining in dentate gyrus of the hippocampus in AAV1-I1\nPP2A, but not AAV1-GFP rats (D).', 'g006Memantine inhibits I1\nPP2A-induced neurodegeneration.']",10.1371/journal.pone.0145441.g006,yes
PMC4078419,Figure_6,oa_package/2b/43/PMC4078419.tar.gz,"['Actually most of the findings at the heel area can be diagnosed clinically, and the role of MSKUS at this part of the body is more of confirming the suspected clinical diagnosis or diagnosing rare findings such as presence of a foreign body (FB) [] and fibroma.', '44-year-old male referred to ultrasound due to painful mass at the heel.']",Figure 6 44-year-old male referred to ultrasound due to painful mass at the heel. Ultrasound shows a subcutaneous foreign body (blue arrow).,yes
PMC3745117,Figure_12,oa_package/9d/cf/PMC3745117.tar.gz,[],Figure 12 A case of turricephaly (upper row) corrected by re-arranging the calvarial segments (middle row). The post-operative appearance 15 years later (lower row),yes
PMC5709537,Figure_4,oa_package/d2/10/PMC5709537.tar.gz,[],Figure 4. Immunohistochemistry of 5xFAD mice with the antibody against phospho-Ser46-MARCKS showed amyloid plaque-like staining pattern. The pattern was not detected in age-matched background mice.,yes
PMC10359737,Figure_3,oa_package/c0/bd/PMC10359737.tar.gz,[],"Fig. 2 Current understanding of the molecular control and fate of hypertrophic chondrocytes in the developing growth plate Schematic diagram illustrating the molecular control and cellular changes focusing on the role and fate of hypertrophic chondrocytes in the process of endochondral ossification. The importance of Runx2 as a transcriptional factor functioning at all stages, from the initiation of chondrocyte hypertrophy to transition to an osteoblast. The plasticity of hypertrophic chondrocytes is highlighted with re-differentiation under cellular stress signals such as the unfolded protein response (UPR). The concept of asymmetric cell division for both cell shrinkage and apoptosis is included, as well as the proposed transition of a hypertrophic chondrocyte to a skeletal stem and progenitor cell (SSPC), with self-renewal ability and differentiation to osteoblast or adipocyte. The role of matrix remodeling at the chondro-osseous junction is also highlighted where the cross talk between hypertrophic chondrocytes and the invading blood vessels brings in essential ingredients (eg. calcium, matrix vesicles, and MMP9 which is supplied by endothelial cells, osteoclasts and hypertrophic chondrocytes). This interplay ensures a controlled conversion of the hypertrophic cartilage to bone, wherein osteoblasts can be derived from hypertrophic chondrocytes and other sources such as the pericytes from the blood vessel, and mesenchymal cells in the bone marrow.",yes
PMC3559224,Figure_2,oa_package/2f/dc/PMC3559224.tar.gz,['05) ().'],"FIG. 2. Cardiomegaly of exercised hearts occurs via hyperplasia, as indicated by an increased number of cardiomyocytes per cross-sectional area. There were 8% more CM nuclei per measured cardiomyocyte (CSA) in EX ventricular sections than Control. CSA was measured as a 0.5mm -box overlaid on standardized dimension digital micrographs. The digital micrograph is a representative Control heart section. Images were created by overlaying the fluorescent image of the section with the DAPI channel (blue, for all nuclei) on top of the image obtained with the TRITC channel (red, for cardiomyocytes). There were 5.82CM nuclei/CSA in EX hearts, versus 5.37 in Control hearts. =9 hearts for EX and 8 hearts for Control. For each heart, CM nuclei counts were performed on sections from two different tissue depth levels, and then averaged together. Error bars represent SEM. Statistical significance: * <0.05.",yes
PMC8357321,Figure_7,oa_package/ab/4c/PMC8357321.tar.gz,['Endomicroscopy and histology of stromal tissue.'],Fig. 7 Endomicroscopy and histology of stromal tissue. Images (a)(d)represent en face endomicroscopic appearance and sections (e)(f)display the corresponding tissue locations after histological cross sectioning and H&E staining. Scale bars are and dashed lines indicate the absolute imaging depth provided with images (a)(d).,yes
PMC4977389,Figure_2,oa_package/ba/d9/PMC4977389.tar.gz,"['Importance of TKRs in Lung CancerAlterations in TKRs have been detected in every histological type of lung cancer (Table 1, ), with a potential role in the development of this disease.', '001""/>TKR alterations in lung cancer studies.']","Figure 2 TKR alterations in lung cancer studies. Graphs showing the frequency of alterations in TKRs of relevance in the different lung cancer histologies found in different studies publicly available at . The different studies are designated by capital letters: (A) Imielinski et al., 2012 [ ]; (B) MSKCC (Memorial Sloan Kettering Cancer Center) 2015; (C) TCGA, 2014 [ ]; (D) Ding et al., 2008 [ ]; (E) TCGA, 2012 [ ]; (F) Peifer et al., 2012 [ ]; (G) Rudin et al., 2012 [ ]; and (H) George et al., Nature 2015 [ ]. Only studies A, C, and E have information about copy number alterations. ADC: lung adenocarcinoma; SCC: lung squamous cell carcinoma; SCLC: small-cell lung cancer.",yes
PMC8678130,Figure_2,oa_package/24/84/PMC8678130.tar.gz,[],"Figure2 The characterization of 4T1-R cells. The cellular water efflux rate constant, k (s ), determined 24h after doxorubicin treatment at different drug concentrations (0.1-5 M). k values were determined by measuring the water proton relaxation times of cell suspensions in the presence of 10 mM of Gd-HPDO3A at 25C on a fixed frequency spectrometer operating at 0.5 T. Data are expressed as the mean SD with n= 4. Percentage of viable cells as determined using the MTT test and plotted vs Log[Doxorubicin] M. Data are expressed as the mean SD of three independent experiments. Cellular uptake of Rb (mol of Rb/mg of protein) measured 24h after doxorubicin treatment. The cells were suspended in a medium containing RbCl 0.12 mM for 1h at 37C and 5% of CO . Data are expressed as the mean SD of three independent experiments. Determination of the % of apoptotic cells after doxorubicin treatment at different concentrations. The percentage of apoptotic cells was assessed by DAPI-, AnnV-APC+ staining using FACS analysis. Data are expressed as the mean SD of three independent experiments.",yes
PMC11102118,Figure_7,oa_package/63/77/PMC11102118.tar.gz,"[' 7 illustrates the study design for the collection of biological samples.', '', ' 7Collection of biological samples at T0 (pre-procedure), T1 (post-procedure) and T2 (after 1 year) in the prospective studyDiscussionThis study provides a comprehensive analysis of procedure-specific radiation data for specific interventional catheterizations in the Italian cohort of patients with CHD, contributing to the HARMONIC project.']","Fig.7 Collection of biological samples at T0 (pre-procedure), T1 (post-procedure) and T2 (after 1year) in the prospective study",yes
PMC6237337,Figure_6,oa_package/ff/3e/PMC6237337.tar.gz,"['Importantly, CSRNP1 protein was present from 1 to 24 h (A 6C) and appeared to be exclusively nuclear (both soluble and chromatin-bound fractions) following subcellular fractionation (C), peaking at 3 h (B and 6D).', 'g006IL-1+OSM induces nuclear expression of CSRNP1.']",10.1371/journal.pone.0207240.g006,yes
PMC9745559,Figure_8,oa_package/80/06/PMC9745559.tar.gz,['Cramp inhibits Serca1 activity and promotes calpain activity in skeletal muscle.'],"Figure 8 Cramp inhibits Serca1 activity and promotes calpain activity in skeletal muscle. (A) In vitro inhibition of ATPase activity by Cramp. SR membrane fractions were incubated with the indicated peptides for 1h and Serca1 activity was measured using increasing concentrations of Ca (pCa 63.5) and 1mM ATP. GA muscles of 8weekold C57BL/6 male mice were used to obtain enriched SR membrane fractions. (B) Elevation of Serca1 protein levels by Cramp haploinsufficiency and deficiency in TA muscles of 24weekold mdx/Utrn male mice. The levels of Ca handling proteins were determined by Western blot analysis using the indicated antibodies and were normalized to Gapdh. (C) Restoration of Serca1 ATPase activity by Cramp HET and HOMO KO in mice with DMD. ATPase activity was determined in pooled homogenates ( ) or individual homogenate ( ) of TA muscles from 24weekold male mice with the indicated genotypes. WT, 24weekold C57BL/6J. =9 for Cramp WT, =10 for HET KO and =10 for HOMO KO. (D) Reduced calpain activity in Cramp KO DMD muscles. TA muscles of 24weekold male mice were used. =4 for each group. (E) Decreased Ca dependent ATPase activity ( ) and increased calpain activity ( ) in Cramptransduced TA muscles. Muscle homogenates from 8weekold C57BL/6 male mice injected with control HP (220) or Cramp for the indicated periods were analysed.",yes
PMC6683384,Figure_1,oa_package/db/48/PMC6683384.tar.gz,"[' 1).', 'Dermal gangrene in three TAO patients who received long-term angiogenic treatment.', 'The patient did not stop smoking during the treatmentDuring histopathological evaluation, we detected some changes in the amputee TAO patients under long-term angiogenic medical treatment that were not present in amputee TAO patients who had not received any treatment for many years.']","Fig. 1 Dermal gangrene in three TAO patients who received long-term angiogenic treatment. , A 30-year-old man with a history of left below-knee amputation due to Buergers disease reported to us with severe Raynauds phenomenon in the right foot that had lasted for a month. He had undergone treatment with Prostavasin and, because of the dependency of the symptoms for Prostavasin perfusion, he underwent right lumbar sympathectomy, followed by treatment with bosentan. However, the pain diminished, and the foot warmed up for only 3 weeks after sympathectomy. By increasing the pain and discoloration of the foot, the patient received iloprost, followed by cilostazol for 7 months. Although the foot warmed and the dorsalis pedis pulse became palpable, the skin began to experience necrosis, whilst granulation tissue appeared under the dermal gangrene. Due to progression of the gangrene, the patient underwent a second BK amputation. The patient had stopped smoking during the treatment, according to self-report. , A 42-year-old man with an eight-year history of Buergers disease reported with a non-healing ulcer on his right ankle and burning pain in the right toes. He received antibiotics as well as cilostazol. The ulcer on the ankle completely healed over the course of 4 months. However, 2 months later, the patient developed a punched-out ulcer on the dorsum of the foot as well as some purpuric-like lesions, which became gangrenous and led to progressive dermal gangrene over the course of 6 months. Finally, the patient underwent below-knee amputation after 1 year of medical treatment. The patient had stopped smoking during the treatment, according to self-report. A 39-year-old man with a four-year history of Buergers disease underwent minor amputation and reported with a non-healing ulcer of the amputation stump. He was treated with cilostazol for approximately 2 years, and the ulcer improved. Recently, he developed localised, gangrenous papules and was advised to discontinue cilostazol. He has stopped smoking, according to self-report , A 49-year-old man with a 15-year history of Buergers disease began taking cilostazol for claudication. After 1 year, although the claudication had improved, localised dermal gangrene developed, and the cilostazol was discontinued. At present, the gangrene has resolved, but the claudication had progressed. The patient did not stop smoking during the treatment",yes
PMC4063936,Figure_5,oa_package/67/60/PMC4063936.tar.gz,"['Mice infected with Af293 displayed larger foci of inflammation and fungal growth that extended further into the lung parenchyma, whereas Af5517 infection and resultant inflammation was more bronchiocentric (A).', 'When lung fungal growth was quantified in Gomori s Methanamine Silver (GMS) stained sections, areas of fungal growth were significantly larger in Af293-infected mice with both doses tested (B).', 'g005Histopathology of Af293 and Af5517 infection.']",10.1371/journal.pone.0100430.g005,yes
PMC3150648,Figure_2,oa_package/74/37/PMC3150648.tar.gz,"[' 2).', 'On T1-weighted image with fat suppression, the lesion showed a low-intensity signal (a), while on T1 coronal turbo-spin-echo (TSE) weighted image (b), a heterogeneous high-intensity signal was observedThe work-up diagnosis was primary GCT, which was confirmed by trucut biopsy.']","Fig.2 MRI of the wrist. On T1-weighted image with fat suppression, the lesion showed a low-intensity signal ( ), while on T1 coronal turbo-spin-echo (TSE) weighted image ( ), a heterogeneous high-intensity signal was observed",yes
PMC9108784,Figure_4,oa_package/3a/4b/PMC9108784.tar.gz,"['The patient is then shifted to the IR suite, and angiography is done with embolization if necessary ().']","Figs 4A and B A 28-year-old lady with a history of previous LSCS was diagnosed with placenta accreta spectrum during the antenatal check-up. Because of expected PPH, it was decided to prophylactically place balloon catheter in both the internal iliac artery prior to labor induction. (A) Fluoroscopic image shows the tip of balloon catheter (black arrows) in internal iliac arteries on both sides. The outline of the fetal spine can be seen faintly (curved arrow); (B) Post-delivery, the balloon catheter was inflated (white arrows) and shifted to the IR suite for uterine artery embolization to control PPH. Dr Bhavesh A Popat, KEM Hospital",yes
PMC5626808,Figure_1,oa_package/7a/23/PMC5626808.tar.gz,"['(A,B) Axial and coronal FLAIR MRI at 4 years of age showed normal findings.']","Figure 1 Axial and coronal FLAIR MRI at 4years of age showed normal findings. Axial T2-weighted MRI and coronal FLAIR MRI at 6years of age showed no significant interval change. FLAIR, fluid attenuated inversion recovery; MRI, magnetic resonance imaging.",yes
PMC4895111,Figure_2,oa_package/22/42/PMC4895111.tar.gz,"['Further experiments in THP-1 macrophages demonstrated that, unexpectedly, knockout of nuoG from BCG strains drastically enhanced colocalization of the vaccine with the autophagy protein LC3 (', '(', ' 2A and B).', 'FIG 2 Deletion of nuoG from BCG and BCG ureC::hly leads to increased association of LC3 with engulfed bacteria in THP-1 macrophages.']","FIG2 Deletion of from BCG and BCG :: leads to increased association of LC3 with engulfed bacteria in THP-1 macrophages. (A) LC3 staining (green) in THP-1 macrophages infected with PKH26-labeled BCG SSI, BCG , BCG :: , and BCG :: (red), 24h p.i. Nuclei are stained with Hoechst stain (blue). Results are representative of three experiments. (Top) Bar, 10m; (bottom) cropped images showing a single cell. (B) Autophagy quantification in THP-1 macrophages at 8 and 24h p.i. Individual points represent the percentages of 25 infected cells containing LC3-associated bacteria, and 300 cells were counted per group. Two experiments. Data were analyzed using one-way ANOVA with Tukeys multiple-comparison test. ***, < 0.001.",yes
PMC6749875,Figure_4,oa_package/67/6a/PMC6749875.tar.gz,['Nasofacial angle: it is formed by drawing a vertical line tangent to the forehead at the glabella and the tangent to the chin at the pogon-ion so that a line drawn along the nasal dorsum intersects it ().'],Fig. 4 Nasofacial angle,yes
PMC9057627,Figure_7,oa_package/4b/e6/PMC9057627.tar.gz,"['We performed RNA-Seq of L3 EMM iPSC-aCMs as a time series to examine aCM development, selecting for 3 time points (Supplemental ).', 'In baseline iPSC-aCMs, there was no change in the expression level of any complex (, A and B).', 'When we examined the WT, mutant, and GC cell lines, EMM NPPA-S64R iPSC-aCMs displayed an overall reduction in all 5 complexes (, C and D), indicating that a defect in complex I is responsible for the early development of the metabolic substrate for AF, with this defect leading to a reduction in the expression of the ETC.', 'To assess overall cellular bioenergetics, we functionally assessed OCR and demonstrated that EMM NPPA-S64R iPSC-aCMs displayed significantly reduced oxidative capacity, and EMM NPPA-S64R-GC displayed a near-complete recovery in oxidative capacity to that in WT (, E and F).', 'Metabolic substrate underlying NPPA-S64R mutation.']","Figure 7 Metabolic substrate underlying S64R mutation. ( and ) Western blot of mitochondrial oxidative phosphorylation proteins in baseline -WT, baseline -S64R, and baseline -S64R-GC iPSC-aCMs shows no significant difference in the expression of any of the 5 complexes involved in the electron transport chain (ETC). ( and ) Western blot of mitochondrial oxidative phosphorylation proteins in EMM -WT, EMM -S64R, and EMM -S64R-GC iPSC-aCMs shows that expression of the each of the 5 complexes is significantly reduced in -S64R iPSC-aCMs, indicating the necessity of utilizing matured iPSC-aCMs; ( = 2 batches, = 3 biological replicates per batch). ( and ) Real-time oxygen consumption rate (OCR) measurements of EMM -WT, EMM -S64R, and EMM -S64R-GC iPSC-aCMs show that -S64R iPSC-aCMs display significantly reduced respiration rate under basal conditions and after mitochondrial coupling. ( = 2 batches, = 24 biological replicates per batch); * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001 (2-way ANOVA with Bonferronis correction).",yes
PMC4031361,Figure_1,oa_package/eb/ca/PMC4031361.tar.gz,"[' 1).', 'Table 2Current clinical trials on MRI conditional pacing devicesMedtronicSt Jude MedicalBiotronikBostonStudyAdvisa IIAccentProMRI AFFIRMSamuraiNumber of participating sites40802145Estimated number of patients270800245363Implantable pulse generator leadAdvisa 5076Accent TendrilEvia/Entovis Safio SImage Ready SystemEstimated date of completionOctober 2014July 2014December 2013July 2014\nSteady state free precession CINE images.', 'Note that the image quality in the short axis cine (b) is sufficient to allow reliable calculation of left ventricular ejection fraction\nBiventricular (CRT) devicesThe presence of three leads in patients treated with biventricular (CRT) therapy increases the risk of interactions between MRI and the pacing system.']",Fig. 1 Steady state free precession CINE images. Typical artefacts as can be observed in patients with an implanted impulse generator ( ) and pacing leads ( ). Note that the image quality in the short axis cine ( ) is sufficient to allow reliable calculation of left ventricular ejection fraction,yes
PMC8320374,Figure_5,oa_package/32/82/PMC8320374.tar.gz,[],"Figure5 Innate immune cells protect mice from DSS-induced colitis upon IL-33 treatment. To investigate the role of innate immune cells, DSS was administered to mice for 6 days, followed by one day of normal drinking water. Mice were injected i.p. either with PBS or with IL-33 on day 0, 2 and 5 (n=3-9 mice per group). Body weight change and disease activity index were monitored daily. Histopathological score of PBS and IL-33-treated mice was assessed on day 7, and representative H&E staining of colon sections were analyzed (n = 4). Scale bars represent 100 m. Levels of IL-33, TNF-, IL-6, IL-5 and IL-13 were measured in colonic explants using Luminex technology. Immune cells were isolated from the colonic lamina propria and the frequencies of lineage ICOS ST2 ILC2s were analyzed by flow cytometry. All data are presented as mean SEM. Statistical analyses were performed using two-way ANOVA followed by Tukeys multiple comparison , unpaired Students t-test , or one-way ANOVA followed by Dunns or Dunnetts multiple comparison test . * < 0.05; ** < 0.01; *** < 0.001.",yes
PMC4161001,Figure_1,oa_package/d1/88/PMC4161001.tar.gz,"['Voxel-based group comparison between 19 AD and 22 cognitively normal age-matched control participants (NC), indicating the fornix as the hotspot.']","Figure 1 . Areas with signal or volume alterations in AD compared to NC are shown as colored maps, overlaid on an averaged FA map , an averaged T map , and an averaged GM segmentation map . Areas with reduced FA. Areas with increased MD. Areas with increased . Areas with increased . Areas with an increased and decreased Jacobian, which was calculated from a transformation matrix obtained from the normalization of DTI. Area with increased T . Areas with increased and decreased Jacobian, which were calculated from a transformation matrix obtained from the normalization of a GM segmentation map. Pink arrows with numbers indicate: 1 = the fornix; 2 = the cingulum; 3 = the posterior cingulate gyrus white matter; 4 = splenium of the corpus callosum; 5 = genu of the corpus callosum; 6 = the prefrontal white matter; 7 = the orbitofrontal white matter; the temporal white matter; 9 = the parietal white matter; 10 = the periventricular area; 11 = the thalamus; 12 = the hippocampus; 13 = the entorhinal area; 14 = the parietal cortex; 15 = the area between the caudate head and the gyrus rectus; 16 = the lateral ventricle. White arrows show the misregistration seen in the left posterior horn of the lateral ventricle (From Oishi et al., ; with permission).",yes
PMC8354400,Figure_15,oa_package/24/f2/PMC8354400.tar.gz,[],"Figure 15 Elastolytic giant cell granuloma: (a) Dermis shows areas of elastolysis with formation of ill-formed granuloma (hematoxylin and eosin, x100), (b) Dermis shows fragmented elastic fibers and elastophagocytosis by the giant cells (hematoxylin and eosin, x400)",yes
PMC7752619,Figure_3,oa_package/11/e7/PMC7752619.tar.gz,"['On higher magnification ( 40) (B), the connective tissue seems to contain an inflammatory infiltrate with lymphocytes and macrophagesA and B, Sagittal (A) section of a lumbar spine MRI on T2 WI and axial (B) section of a lumbar spine MRI on T1 WI with injection of gadolinium showing a small recurrent or residual lesion3DISCUSSIONPigmented villonodular synovitis is an uncommon condition, distinguished by synovial proliferation associated with a hemosiderin deposition in the affected joint.']","Figure 3 A and B, Sagittal (A) section of a lumbar spine MRI on T2WI and axial (B) section of a lumbar spine MRI on T1WI with injection of gadolinium showing a small recurrent or residual lesion",yes
PMC11545295,Figure_5,oa_package/d3/ec/PMC11545295.tar.gz,"['Upon stimulation with 500 ng/mL NTN4, the complement component 3 (C3) gene exhibited a significant increase in expression after 24 h (A, p = 0.', '011) post-treatment (B).', 'Similarly, CXCL6 expression was significantly elevated after 6 h (C, p = 0.', '022), and 24 h (D, p = 0.', '042) following NTN4 treatment (E).', 'Matrix metalloproteinase 1 (MMP1), an enzyme involved in collagen degradation, was significantly upregulated after 24 h (F, p = 0.', '022) hours (G), suggesting enhanced cell adhesion and potential immune cell recruitment.', 'qPCR analysis of vehicle and recombinant Netrin-4 treated fibroblastic cells derived from the IFP.']","Figure 5 qPCR analysis of vehicle and recombinant Netrin-4 treated fibroblastic cells derived from the IFP. ( ) ( ) , ( ) , ( ) , ( ) , ( ) , and ( ) . * indicates significant differences between groups at each time point ( < 0.05).",yes
PMC4102570,Figure_7,oa_package/af/ec/PMC4102570.tar.gz,"['jejuni, (a) they proved largely unresponsive to the pathogen, exhibiting few if any signs of the gastroenteritis seen in infected Sigirr\n / mice (b).', 'g007Pathology of Tlr2 / /Sigirr / and Tlr4 / /Sigirr / mice infected with C.', 'jejuni infection, even compared to infected Sigirr / mice (b and c), suffering exaggerated gastroenteritis by 3 DPI that involved worsened edema, crypt hyperplasia and inflammatory cell infiltration, including large numbers of neutrophils.', 'jejuni seen in large numbers deep within cecal and colonic crypts, as well as inside intestinal epithelial cells (c and data not shown).', '1% (66/140) of observed crypts being positively colonized, often with high numbers of bacteria in each crypt (c).']",10.1371/journal.ppat.1004264.g007,yes
PMC11354926,Figure_1,oa_package/16/8a/PMC11354926.tar.gz,"['Uterine NK cells are found in the non-pregnant uterus, in the decidua, and, in mice, also in between the two muscle layers of the uterine wall ().', '01430503213\nUterine NK cells interact with extravillous trophoblasts: Extra villous trophoblasts invade in the spiral arteries, decidua and myometrium of the uterus.']","Figure 1 Uterine NK cells interact with extravillous trophoblasts: Extra villous trophoblasts invade in the spiral arteries, decidua and myometrium of the uterus. Uterine NK cells engage in crosstalk with trophoblast. Trophoblasts are a unique kind of extraembryonic cells that pose interesting immunological challenges [ ].",yes
PMC4431046,Figure_4,oa_package/ec/df/PMC4431046.tar.gz,"['On this STIR study, there also appears to be an increased signal in the cord at this levelAxial CT image at the C4-C5 disc space following the CSS.']","Figure 4 Axial CT image at the C4-C5 disc space following the CSS. This axial noncontrast CT study demonstrated marked stenosis/ spondylosis at the C4-C5 level narrowing down the spinal canal to 56 mm. Ventrally the hyperdense soft-tissue appears more spondylotic vs. soft disc, while posteriorly the compression was attributed to inward shingling of the C5 lamina. Certainly, both the MR and CT studies help document why this patient, with such severe stenosis at the C4-C5 level, was so susceptible to the CSS cord injury",yes
PMC4254453,Figure_11,oa_package/db/05/PMC4254453.tar.gz,[],10.1371/journal.pone.0113317.g011,yes
PMC7369511,Figure_1,oa_package/68/b0/PMC7369511.tar.gz,"['He originally presented in April 2017 with an anal mass and several months of bleeding, and a biopsy of the mass confirmed the diagnosis of anal squamous carcinoma with a positive p16 stain result ().', 'Histopathology of the primary tumor at diagnosis.']","Fig 1 Histopathology of the primary tumor at diagnosis. Biopsies at the original diagnosis display islands of atypical epithelial cells in a background of dermal fibrosis, most evident at 40 ( ). Higher magnification demonstrates nuclear atypia and mitotic figures at 400 ( ). Result for p16 staining was positive, indicative of human papillomavirus infection ( ).",yes
PMC8353499,Figure_6,oa_package/68/8e/PMC8353499.tar.gz,"['Weight regain is mainly due to gastric pouch dilatation, which consists of an abnormal ectasia of the gastric lumen, is detected via CT scan following oral contrast administration; 3D multiplanar measurements and reconstructions are useful for evaluation of the morphology and anatomical relationships of the stomach with other abdominal organs ( 6) [15].', ' 6Gastric pouch dilatation in a 42-year-old woman with weight regain 5 years after MGB/OAGB surgery.']","Figure6 Gastric pouch dilatation in a 42-year-old woman with weight regain 5 years after MGB/OAGB surgery. Axial (a), coronal (b) and sagittal (c) CT images after oral contrast medium administration show an abnormal ectasia of functional gastric lumen (black ).",yes
PMC4158253,Figure_5,oa_package/76/73/PMC4158253.tar.gz,"['There is also extension of the abscess to the anterior perisacral region, with adjacent sacral bony erosions and abnormal marrow enhancement indicating osteomyelitis ().', '004""/>Axial T1-weighted fat-suppressed image postcontrast shows a well-defined rim-enhancing collection (white arrows) at the anterior perisacral space compatible with abscess, as well as abnormal marrow enhancement (thick arrow) in the sacrum consistent with osteomyelitis.']","Figure 5 Axial T1-weighted fat-suppressed image postcontrast shows a well-defined rim-enhancing collection (white arrows) at the anterior perisacral space compatible with abscess, as well as abnormal marrow enhancement (thick arrow) in the sacrum consistent with osteomyelitis.",yes
PMC8250200,Figure_1,oa_package/28/0c/PMC8250200.tar.gz,[],FIGURE 1 (A) Photograph showing erythema on the tips of the toes as well as some desquamation on the 2nd toe and periungal redness. (B) Photograph of registry participant with purple and red macules on the heel. (C) Photograph of participant who had acral changes in March 2020 and then again in the fall of 2020 showing red patches with erosions on the toes including periungual region,yes
PMC11588598,Figure_1,oa_package/68/e1/PMC11588598.tar.gz,"['Small aneurysm was seen arising from the terminal part of the basilar artery (\n\n).', '\nComputed tomography (CT) angiogram showing left superior cerebellar artery aneurysm (\nA\n) and CT brain plain showing subarachnoid hemorrhage (SAH) in cisternal and other subarachnoid spaces along with intraventricular hemorrhage (IVH) (\nB\n).']",Fig. 1 Computed tomography (CT) angiogram showing left superior cerebellar artery aneurysm ( ) and CT brain plain showing subarachnoid hemorrhage (SAH) in cisternal and other subarachnoid spaces along with intraventricular hemorrhage (IVH) ( ).,yes
PMC7399334,Figure_5,oa_package/53/22/PMC7399334.tar.gz,['Different views of the effect of AD ( kAD) in subjects in the MCI stage.'],"Figure 5 Different views of the effect of AD ( ) in subjects in the MCI stage. The color-code represents negative (blue) and positive (red) relative contributions of each CSF biomarker explaining the volumetric variability on brain each ROIs. Each figure represents a different CSF biomarkers: CSF A, CSF -tau, CSF -tau. Only brain ROIs with statistically significant effect size ( < 0.05, corrected for multiple comparisons) are shown.",yes
PMC7417129,Figure_2,oa_package/c9/57/PMC7417129.tar.gz,[],Figure 2 Preoperative axial T2,yes
PMC5475354,Figure_2,oa_package/c8/cf/PMC5475354.tar.gz,"['7) of the left thigh muscles, left gastrocnemius, right tibialis anterior, bilateral foot muscles, and right scrotum, among other areas (, A).', 'Whole-body PET/CT scans (A) before high-dose prednisone regimen (June 2016) and (B) after high-dose prednisone regimen (July 2016).', 'A PET/CT scan without contrast showed disease improvement in the subcutaneous tissues and musculature of all affected areas in the lower extremities (, B).']",Fig 2 Whole-body PET/CT scans ( ) before high-dose prednisone regimen (June 2016) and ( ) after high-dose prednisone regimen (July 2016). Red arrows denote areas of particular interest.,yes
PMC3405480,Figure_2,oa_package/2e/50/PMC3405480.tar.gz,"['Fourteen days after chronic intracerebroventricular infusion of LPS in rats, an altered state of neuronal plasticity and an elevated number of OX-6 positive microglial cells were observed in the hippocampus.']","Figure 2 After 14days co-administration of LPS (i.c.v. [ ]) with 3,6-dithiothalidomide (i.p), no differences in the time spent in the outer and inner zone of an exploration chamber were observed between the treatment groups. In each animal cohort the rodents displayed a preference for the outer zone rather than the inner zone. The mean time spent in the two zones over the first 3min of a 10-min exploratory period is shown (aCSF + Veh =1; =49). The effects of the 10-min behavioral assessment on the immediate early gene. Activity regulated cytoskeletal protein ( ) gene expression in the upper blade of the hippocampal neurons is presented; hybridization data indicate that there was an increase in the number of neurons expressing mRNA in LPS+vehicle-treated animals. The behavior-LPS-induced elevation was prevented by treatment with 3,6-dithiothalidomide ( =49). See Figure for representative images of Arc staining. LPS administration induced a significant increase in numbers of OX-6 positive microglial cells, while treatment with 3,6-dithiothalidomide prevented this effect of LPS. See Figure for representative images of activated microglia staining. Data are expressed as mean SEM of observations; levels of statistical significance are indicated as follows: * <0.05.",yes
PMC6766113,Figure_5,oa_package/81/c5/PMC6766113.tar.gz,"['004""/>The abdominal LMA.']",Figure 5 The abdominal LMA.,yes
PMC3808297,Figure_6,oa_package/98/80/PMC3808297.tar.gz,['g006Circadian oscillation in Nox4 and Nox1 gene expression in cardiac and endothelial cells.'],10.1371/journal.pone.0078626.g006,yes
PMC5908560,Figure_1,oa_package/80/86/PMC5908560.tar.gz,['Chest computed tomography showing massive bilateral pulmonary embolism.'],Figure 1 Chest computed tomography showing massive bilateral pulmonary embolism.,yes
PMC9394199,Figure_1,oa_package/3c/a1/PMC9394199.tar.gz,"['Swelling was studded with multiple papules and plaques with brown-coloured crusts and atrophic scars at few sites (figure 1).', 'Baseline clinical picture showing diffuse skin-coloured swelling present over neck; (A) right lateral view, (B) back view and (C) left lateral view.']","Figure 1 Baseline clinical picture showing diffuse skin-coloured swelling present over neck; (A) right lateral view, (B) back view and (C) left lateral view.",yes
PMC10556328,Figure_5,oa_package/76/79/PMC10556328.tar.gz,"['5% of patients in more distal parenchymal branches, and in accessory renal arteries, respectively (\n\n).', 'org/1999/xlink"" xlink:href=""10-1055-s-0043-1772496-i2271739-4""/>\nAccessory renal artery occlusion in a 15-year-old girl with elevated blood pressures above 95\nth\ncentile: Angiogram showing occlusion of proximal and mid segments of the accessory renal artery to left lower pole with distal filling through collaterals (black arrow).']",Fig. 5 Accessory renal artery occlusion in a 15-year-old girl with elevated blood pressures above 95 centile: Angiogram showing occlusion of proximal and mid segments of the accessory renal artery to left lower pole with distal filling through collaterals (black arrow).,yes
PMC6682391,Figure_1,oa_package/3d/47/PMC6682391.tar.gz,"['2D Digital Diagnostic Mammogram of Left BreastA 37-year-old man with a benign simple breast cyst: 2D digital diagnostic mammogram of left breast, CC (a), MLO (b), CC with spot compression (c), and MLO with spot compression (d) views.']","Figure 1 2D Digital Diagnostic Mammogram of Left Breast A 37-year-old man with a benign simple breast cyst: 2D digital diagnostic mammogram of left breast, CC (a), MLO (b), CC with spot compression (c), and MLO with spot compression (d) views. His diagnostic mammogram demonstrated an oval circumscribed mass within the left breast, just superior and lateral to the nipple. Bilateral gynecomastia is also seen with flame-shaped, subareolar fibroglandular densities.",yes
PMC5821194,Figure_6,oa_package/f2/b5/PMC5821194.tar.gz,"['Consistent with overall M numbers, M1 (defined by CD206 negativity) and M2 (defined by CD206 positivity) M subpopulations increased in iRhom2-deficient mice throughout the first week of repair following MI (, A and B).', 'Interestingly, we observed a significant increase in the M1/M2 M ratio in mutant hearts at day 4 compared with wild-type controls, suggesting a skewed response towards proinflammatory M s; followed by a decrease in the M1/M2 ratio at day 7, indicative of overall more reparative M s (C).', 'However, coincident with this altered cell ratio profile we observed a strong reduction in the overall cell surface expression of the mannose receptor CD206 in M s from iRhom2-deficient mice at day 7 relative to wild-type controls (D).', 'We determined that iRhom2-deficient cardiac M s have decreased expression of proinflammatory cytokines (Il1b and Il6) during the initial inflammatory response (day 2) compared with controls (E), signifying impaired M1 function.', 'In addition, we also observed a decrease in the downstream reparative cytokines (Tgfb and Il10) in iRhom2-deficient M s at later stages of inflammation (day 7) (F).', 'In post-MI iRhom2-deficient M s, the overall ratio of M1/M2 M s residing in injured iRhom2-deficient hearts, as classically defined by the absence or presence of CD206 expression, respectively, was increased at day 4 and decreased at day 7 (C), indicating more M1 and M2 macrophages, respectively.', 'However, the more functionally relevant M1 proinflammatory cytokine signature was significantly dampened (Il1b and Il6) at the initiation of inflammation, associated with a subsequent reduction in CD206 expression and M2 reparative cytokine expression (Il10 and Tgfb) during later stages (, E and F).', 'These data suggest that M polarization towards M1 during the early immune response is mediated by the iRhom2/TNF- proinflammatory signaling axis (33, 34), and this targeted effect impacts on the incidence, polarization, and function of later-stage M2 M s ().', 'M1/M2 macrophage ratios and cytokine expression within the first week after injury.']","Figure 6 M1/M2 macrophage ratios and cytokine expression within the first week after injury. The numbers of ( ) CD206 M1 macrophages (Ms) were significantly increased at days 2 and 4 in iRhom2-deficient (iRhom2 ) hearts compared with controls. Similarly, the numbers of ( ) CD206 M2 Ms were increased significantly at days 2 and day 7 in iRhom2 hearts. ( ) M1/M2 ratios were increased and decreased at days 4 and 7, respectively, in iRhom2 mutants compared with wild type. ( ) The mean fluorescence intensity (MFI) of CD206 M2 Ms was markedly reduced in iRhom2 Ms at day 7 after injury. ( ) Relative mRNA expression (2 ) of proinflammatory cytokines ( and ) from sorted M1 Ms (CD45 CD64 MerTK Ly6G F4/80 CD206 ) was significantly decreased at day 2 after injury compared with wild-type controls. ( ) Gene expression of reparative cytokines ( and ) were significantly reduced in sorted M2 Ms (CD45 CD64 MerTK Ly6G F4/80 CD206 ) at day 7 after injury, compared with wild-type hearts. Data are shown as the mean SEM, = 5 for each experimental group. * 0.05, ** 0.01, *** 0.001, 1-way ANOVA and post-hoc test.",yes
PMC8006826,Figure_1,oa_package/d0/82/PMC8006826.tar.gz,"['A widespread rash that was erythematous, raised and pruritic and consistent with urticarial rash was noted on her bilateral upper extremities and lower extremities (figure 1 and figure 2).', 'Diffuse urticarial rash on bilateral lower extremities.']",Figure 1 Diffuse urticarial rash on bilateral lower extremities.,yes
PMC10556189,Figure_3,oa_package/61/41/PMC10556189.tar.gz,"['Microscopic evaluation of the liver 6 weeks post dose with scAAV9-SMN1 revealed the presence of minimal or slight oval cell hyperplasia ( 3A), which resolved at the 6-month time point.', 'All scAAV9-related microscopic findings occurred at a similar incidence and/or severity with or without co-administration of prednisolone (no effect on incidence, severity, or distribution of microscopic findings; 3B), and general incidence and/or severity were more severe 6 weeks post dosing than at 26 weeks.', ' 3Histopathological and molecular marker (IHC/ISH) changes after IV administration of a mid-dose concentration, 1 1014 vg/kg, of scAAV9-CBA-SMN1(A) Representative images of minimal oval cell hyperplasia (green arrows) with slight mononuclear cell infiltrate (yellow arrowheads) after IV delivery of scAAV9-CBA-GFP without prednisolone (200 magnification).', '1 1014 vg/kg scAAV9-CBA-SMN1, with animals co-administered prednisolone having slightly higher AS and S signals ( 3C).', 'At 6 weeks post dosing, animals administered scAAV9-CBA-SMN1 without prednisolone had an increase in the number of CD3-positive T cells in the periportal regions and as small aggregates within the hepatic parenchyma ( 3D) compared with vehicle control animals.', 'Immunohistochemistry (IHC) staining to detect the adenosine deaminase acting on RNA 1 (ADAR1), an enzyme involved in innate immune responses through editing of double-stranded RNA and known to negatively regulate the RLR pathway, revealed that animals administered scAAV9-CBA-SMN1 without prednisolone had an increased signal in the hepatic parenchyma 6 weeks post dose ( 3E).']","Figure2 Timeline of key clinical pathology changes after IV administration of a mid-dose concentration, 1.110 vg/kg, of scAAV9-CBA- (A) ALT, (B)platelet, and (C)monocyte concentrations over time. ALT, alanine aminotransferase. See also , Study E.",yes
PMC5644330,Figure_3,oa_package/34/0e/PMC5644330.tar.gz,['The axillary artery is normal (upper white arrow)(A and B)(A) The anterior projection at the level of the hand reconstitution of the radial artery distal to occlusion (red arrow and short white arrows).'],"Figure 3(A and B) (A) The anterior projection at the level of the hand reconstitution of the radial artery distal to occlusion (red arrow and short white arrows). Deep palmar arch (arrowheads) through the ulnar artery (long arrow on the right side) that explains the reason for normal Allen test in this patient; (B) Unenhanced computed tomography shows diffuse and dystrophic calcification of bilateral radial and ulnar arteries (arrows). Computed tomographic angiography shows patency of proximal ulnar and radial arteries, diffusely calcified segments cannot be evaluated",yes
PMC8177037,Figure_5,oa_package/58/0b/PMC8177037.tar.gz,"[' 5) and the measurement of extracellular volume using contrast-enhanced imaging, is a method to quantitatively evaluate T1 relaxation time influenced by amyloid deposits [74, 75].', 'Myocardial T1 mapping of magnetic resonance imaging in a late-onset ATTRVal30Met amyloidosis patient from a nonendemic area of Japan.', 'Native myocardial T1 mapping indicates abnormally increased T1 relaxation timeAs described earlier, the usefulness of strain imaging for the diagnosis of cardiac amyloidosis has been established by ultrasonography techniques.']",Fig. 5 Myocardial T1 mapping of magnetic resonance imaging in a late-onset ATTRVal30Met amyloidosis patient from a nonendemic area of Japan. Native myocardial T1 mapping indicates abnormally increased T1 relaxation time,yes
PMC11407497,Figure_7,oa_package/59/85/PMC11407497.tar.gz,"['The MCF-7 homotypic spheroids were loose and had irregular shapes (A), and their integrity was easily lost at the moment of pipetting (data not shown).', 'The volume of homotypic MCF-7 spheroids increased during the days of monitoring, reaching a plateau in growth at day 14 (B).', 'This plateau was confirmed by the decrease in the speed of growth for the second week of growth (C).', 'The Hs27 fibroblasts compacted the MCF-7 epithelial spheroids (D), reflected by the decrease in volume in the case of heterotypic spheroids (E).', 'MCF-7 spheroids.']","Figure 7 MCF-7 spheroids. A, Sequential images of a homotypic MCF-7 spheroid taken at days 1, 2, 4, 7, and 14 post-seeding, with edges highlighted by OrganoSeg in blue; B, Graph showing the changes in the volume of homotypic spheroids over time from the day of seeding; C, Evaluation of spheroids growth as the speed of volume increase per day, for different periods during the formation of homotypic type; D, Images at day 7 of a homotypic and a heterotypic spheroid; E, Comparison in volumes at day 7 between homotypic and heterotypic spheroids (MCF-7/Hs27, 1/1).",yes
PMC11350933,Figure_2,oa_package/f2/20/PMC11350933.tar.gz,"[' 2C, D).', '', 'CT imaging (C) and chest x-ray (D) showing cement in the right lower lobeDiscussionTo summarize our case, a 74 year-old woman who presented to the ED with chest pain, back pain, and dyspnea was found to have a right lower lobe pneumonia and a cement extravasation into the IVC after kyphoplasty L4 L5 2 days prior to initial presentation.', ' 2 C, D).', '2 C, D).']",Fig.2 Readmission images demonstrating intravasation of the cement. CT imaging ( ) and chest x-ray ( ) showing cement in the right lower lobe,yes
PMC6212930,Figure_4,oa_package/98/d6/PMC6212930.tar.gz,"['The percentage of CD4+ and CD8+ T cells in STDME-fed mice were unchanged compared with controls (A).', 'Interestingly, however, the percentage of CD4+ CD25+ Foxp3+ Tregs in STDME-fed mice was higher than that of the control (B), suggesting that the oral administration of STDME suppresses the attenuation of T cell function associated with CAC.', 'Splenic T cell subsets in STDME-fed mice after induction of AOM/DSS-induced CAC.']","Figure 4 Splenic T cell subsets in STDME-fed mice after induction of AOM/DSS-induced CAC. Splenocytes from control or STDME-fed CAC-induced mice were isolated. Representative dot plot of CD4 , CD8 and CD4 CD25 Foxp3 splenocytes analyzed by flow cytometry. Each bar represents the mean SEM and the data were assessed by a Mann-Whitney -test. * < 0.05; ns = not significant; = 10/group. STDME, selenoneine-containing tuna dark muscle extract.",yes
PMC5726568,Figure_4,oa_package/61/63/PMC5726568.tar.gz,"['Ultrastructural abnormalities were present in both large vessels and capillaries of the NAc (Supplementary D).', 'No significant change was observed in the PFC of SS or RES mice when compared to unstressed CTRL mice (Supplementary E).', 'Half of the brain was collected for flow cytometry analyses and the other half fixed for immunohistochemistry (IHC) (A).', '0103) (B and Supplementary ', '0479) (C), with no change in ccr2RFP /cx3cr1GFP+ resident microglia (Supplementary ', '0321) (D), although this does not rule out the possibility that stress induces local production of IL-6.', '45 kDa) and injected it retro-orbitally into the blood of CTRL, SS or RES mice (G and Supplementary ', 'Interestingly, biotinylated-IL6 was only detectable in the NAc of SS mice and labeling with the perivascular marker CD31 revealed passage outside of blood vessels into the parenchyma (G).', 'Importantly, our studies also revealed that the NAc is largely impermeable to circulating IL-6 under normal conditions as we were unable to detect biotinalyted-IL6 in the NAc of unstressed CTRL mice (G) and i.', 'injections of recombinant IL-6 did not increase levels of IL-6 in NAc of naive mice (E) despite there being a large increase in the blood of these mice (Supplementary ', 'Importantly, downregulation of cldn5 expression with an AAV-shRNA allows the passage of biotinylated-IL6 in the NAc parenchyma (H).', 'To confirm the importance of NAc IL-6 in the establishment of depression-like behaviors, cannula were implanted in the NAc of stress-na ve mice and either saline or IL-6 was infused before subthreshold micro-defeat (F).', '0405) (F and Supplementary ', 'Chronic social stress induces peripheral monocyte accumulation and IL-6 passage into the NAcA) Experimental timeline of 10-day CSDS, SI behavioral screening and tissue collection of ccr2RFP cx3cr1GFP mice.']","Figure 4 Chronic social stress induces peripheral monocyte accumulation and IL-6 passage into the NAc Experimental timeline of 10-day CSDS, SI behavioral screening and tissue collection of mice. Stressed mice showed decreased SI scores following 10-day CSDS. Representative heat maps are shown on the right. Flow cytometric analysis of forebrain ccr2 and cx3cr1 cells revealed higher level of ccr2 peripheral monocyte accumulation into the brain of stressed mice. Cells were gated on live CD11b F4/80 (left). Numbers adjacent to gates represent percentages of ccr2 cx3cr1 cells among CD11b F4/80 cells. Right panel shows CD45 expression of F4/80 ccr2 monocytes and F4/80 gfp microglia. Bar graph shows percentage of ccr2 cx3cr1 cells among forebrain CD11b F4/80+ cells. IL-6 protein level is increased in the blood and NAc of SS mice after 10-day CSDS. Dose response IL-6 i.p. injection did not change IL-6 protein level in the NAc of stress-nave mice. Direct infusion of IL-6 into the NAc (5 min) induces social avoidance when subthreshold micro-defeat was conducted 20 min after the end of the infusion. Experimental timeline of peripheral biotinylated-IL6 injection after 10-day CSDS. IL-6-biotin-Neuravidin-Oregon 488 was detectable in the NAc parenchyma of SS mice only after 2h circulation and 5-min perfusion with PBS. Biotinylated IL-6 was also detectable in the NAc of AAV-shRNA- -injected mice. Data represent mean SEM, number of animals ( ) is indicated on graphs. T-test for CX3CR1-GFP/CCR2-RFP mouse data, one-way ANOVA followed by Bonferronis multiple comparison test for IL-6 protein levels and two-way ANOVA followed by Bonferronis multiple comparison test for SI ratio after IL-6 or saline infusion, * < 0.05; ** < 0.01; *** < 0.001.",yes
PMC4921724,Figure_4,oa_package/13/e2/PMC4921724.tar.gz,"['Hair line pixels are replaced with values calculated on the basis of the neighborhood pixels ().', '003""/>Outcome of the preprocessing step: (a) dermoscopic input image and (b) image after black frame removal and hair inpainting.']",Figure 4 Outcome of the preprocessing step: (a) dermoscopic input image and (b) image after black frame removal and hair inpainting.,yes
PMC10182116,Figure_3,oa_package/7b/03/PMC10182116.tar.gz,"[' shows an exemplary comparison of mammograms and PDFF maps of all four ACR categories.', 'Mammograms with breast density estimation following the American College of Radiology Breast Imaging Reporting and Data System categories A-D with corresponding PDFF maps']",Fig. 3 Mammograms with breast density estimation following the American College of Radiology Breast Imaging Reporting and Data System categories A-D with corresponding PDFF maps,yes
PMC6344797,Figure_2,oa_package/4b/ed/PMC6344797.tar.gz,"['SOX10 expression indicates cell of originSOX10 protein showed nuclear expression by IHC ().', 'Mucinous acinar cells were SOX10 negative, whereas serous acinar cells were SOX10 positive (A).', 'In our study, the staining intensity of SOX10 was low in normal serous acinus compared with intercalated ductal cells and intercalated duct-originated tumors, and mucinous acinar cells did not express SOX10 (A).', '.']","Fig. 2. Representative images of SOX10 protein expression. (A) SOX10 staining is not found in mucinous acinar cells. (B) SOX10 positivity is observed in adenoid cystic carcinoma. (C) SOX10 staining is not observed in mucoepidermoid carcinoma, low grade.",yes
PMC5721666,Figure_1,oa_package/1b/a1/PMC5721666.tar.gz,"[' 1) included an infiltration of inflammatory cells, such as neutrophils and lymphocytes at 3 dpi.', 'of animalsInflammatory infiltrationRBCsEosinophilsNeutrophilsFibroblastsLymphocytesMonocytesI05 II35+ + + III105++++ + IV285++++ ++V425+++++++VI705+++++++VII984+++++++\nHistopathological characteristics of the livers of buffaloes infected with Fasciola gigantica.', 'Scale-bars: a-g, 50 m; h, 100 m\nGene expression of cytokinesTo determine how immune response changed over the course of infection, gene expression of nine cytokines was assessed in the liver tissue of 29 infected buffaloes and five uninfected buffaloes using qRT-PCR.']","Fig. 1 Histopathological characteristics of the livers of buffaloes infected with . At 3 dpi, there was local hyperemia associated with mild filtration of lymphocytes and neutrophils. At 10 dpi, there was scattered vacuolation of hepatocytes, consistent with fat, along with mild to moderate focal necrosis. At 28 dpi, diffuse intravascular coagulation, severe infiltration of inflammatory cells mainly neutrophils and lymphocytes, and granular degeneration of the cytoplasm were observed. At 42 dpi, moderate to severe multifocal hemorrhages and necrosis, infiltration of eosinophils, RBCs and monocytes, and accumulation of fibroblasts were detected. At 70 dpi, there were mild to moderate multifocal bile duct hyperplasia and focal coagulative necrosis associated with collagen deposition. At 98 dpi, severe periportal fibrosis associated with multifocal inflammatory infiltrate, cellular debris, moderate multifocal hemorrhages, and granulomas with necrotic centres were detected. Liver tissue from uninfected animal showed normal histological architecture of the hepatic tissue. Adult flukes in the intrahepatic bile duct, along with epithelial hyperplasia of the duct. In all figures, tissue sections were stained with H&E and arrows point at the corresponding morphological features described above. : , 50m; , 100m",yes
PMC7001080,Figure_3,oa_package/6d/61/PMC7001080.tar.gz,"['As shown in 3A, both the parental Abs and the biAb were still detectable in blood on day 14, albeit at low levels, with a half-life of approximately 30 h (Table S2).', 'The parental Abs and the biAb were also detected in various muscle groups, such as the quadriceps, TA, diaphragm, and heart for up to 14 days (see 3B, showing diaphragm and quadriceps as representative examples), indicating that the tissue half-life of the biAb was longer than that in the blood.', 'There was little to no signal in the liver or spleen on day 4, the earliest collection time ( 3C), suggesting that the longer half-life of the Abs in the muscle was due to binding to their respective cellular targets.', ' 3Biodistribution of Abs In Vivo(A) Concentration of the parental Abs and biAb in serum after a single dose of the biAb (30 mg/kg) or a mixture of the parental Abs (15 mg/kg for each mAb).', 'Since the biAb was detected in tissue for more than 14 days ( 3B), the longevity of the noted efficacy was also evaluated.', 'However, attempts to visualize this physical interaction by immunofluorescence imaging were unsuccessful due to the close proximity of the sarcolemma to the basal lamina ( 3B).', 'Blood half-life of antibodies was estimated according to the graph in 3A.']","Figure2 Localized Intramuscular Injection of the biAb into LARGE Mice Protected TA Muscles from Exercise-Induced Damage (A) Muscle lysates of non-treated LARGE and wild-type mice show the lack of glycosylation of DG in the LARGE mice by a IIH6-specific antibody and with a polyclonal antibody recognizing both DG and DG. (B) Muscle lysate IP from biAb, parental-Ab-, or saline-treated animals shows that biAb treatment does not disrupt the dystroglycan complex as shown by pulldown with the bound antibody (left panels) or a DG antibody that has a different epitope than the treatment (right panels; clone 43DAG1/8D5). (C) Summary of EBD-positive fiber counts in biAb- and parental-Ab (control)-treated TA muscles. (D) Representative muscle sections of biAb- and parental-Ab-treated TA muscles. Arrows indicate EBD-positive fibers (orange fibers), and arrowheads represent Ab staining at the sarcolemma (green). Statistical analysis was performed using Students t test; *p< 0.05. n= 8 animals per group, 6 sections at different planes were assessed per TA. Images were taken at 10 magnification.",yes
PMC11353742,Figure_4,oa_package/17/f1/PMC11353742.tar.gz,"['Clefts in the myometrium () were observed in 12 (36%) hysterectomies for adenocarcinoma and in four (15%) benign pathologies.', 'Myometrial clefts filled by tumor cells.']",Figure 4 Myometrial clefts filled by tumor cells.,yes
PMC4423901,Figure_9,oa_package/89/0a/PMC4423901.tar.gz,"['In addition, macroscopic and histological examination showed that the lungs of TNF-a mice exhibited massive inflammatory responses after one month of infection with reduced free alveolar space () and enhanced bacterial load as assessed by Ziehl-Neelsen staining ().', 'g009Controlled lung pathology after M.']",10.1371/journal.pone.0126058.g009,yes
PMC8297485,Figure_2,oa_package/dc/bc/PMC8297485.tar.gz,"['The most common initial manifestations of IPA include solid macronodules ( 1 cm in diameter, present in 94% of patients) with a surrounding halo of ground-glass attenuation classically known as the halo sign (present in 61% of patients) () [6,15-18].', 'Aspergillus pneumonia.']","Figure 2 pneumonia. A) 56-year-old patient with acute myelogenous leukaemia undergoing pre-transplant conditioning presented with neutropenic fever diagnosed with pneumonia. Axial chest computed tomography (CT) shows multiple bilateral solid nodules (arrows) with adjacent ground-glass opacities (arrowhead). Small left pleural effusion is also present (star). B-E) Different patient with invasive pneumonia. A 60-year-old woman with history of acute myelogenous leukaemia status post allogenic stem cell transplant, who presents with persistent fever and pancytopaenia. B) Frontal chest radiograph shows focal peripheral consolidation in the left upper lobe (arrows). C-D) Axial and coronal CT scan show focal consolidation in the left upper lobe (arrow) with adjacent ground glass opacities (arrow heads). E) Coronal CT scan after 3 weeks of antifungal treatment shows decreased size of the focal consolidation (arrow) and the presence of air crescent sign (arrowhead) as part of response to treatment. F) Histopathological microscopy image demonstrating acutely branching, dark, elongated hyphae of",yes
PMC6079293,Figure_3,oa_package/cc/99/PMC6079293.tar.gz,['Representative images showing quantification procedure using NewCAST Visiopharm software.'],Figure 3 Representative images showing quantification procedure using NewCAST Visiopharm software. A: Super image capture of a slide; B: Computer-assisted drawing of the tissue (green) and manually drawing of the regions of interest (ROI) or portal areas (blue); C: Example of the unbiased sampling; D: A detail of cell quantification inside the counting frame.,yes
PMC11577143,Figure_1,oa_package/0f/f8/PMC11577143.tar.gz,['A: Gross image of buttock and rectum demonstrating violaceous prolapsed juvenile rectal polyp (white arrow).'],Figure 1 A: Gross image of buttock and rectum demonstrating violaceous prolapsed juvenile rectal polyp (white arrow). B: Excised prolapsed juvenile polyp measuring 2.3 x 1.6 x 0.8 cm (black arrow),yes
PMC9513194,Figure_5,oa_package/c1/55/PMC9513194.tar.gz,[],FIGURE 5 Surgical pathology of thyroid gland demonstrating papillary and follicular hyperplasia at a low power magnification,yes
PMC6296722,Figure_2,oa_package/d8/4e/PMC6296722.tar.gz,"['EGD revealed diffuse, friable, edematous, polypoid protrusion of the duodenal mucosa with gastric outlet obstruction (); biopsy confirmed AL amyloidosis (a and 3b).', 'EGD showing friable, polypoid protrusion of the duodenal mucosa.']","Figure 2 EGD showing friable, polypoid protrusion of the duodenal mucosa.",yes
PMC3134682,Figure_3,oa_package/b9/a4/PMC3134682.tar.gz,"['1-cm left adrenal nodule () and absent adrenal gland on a right side consistent with a new contralateral hyperfunctioning left adrenal adenoma.', '.']",Figure 3. CT of the abdomen with contrast (2 years after right adrenalectomy) demonstrates a new 1.1-cm nodule with nonspecific characteristics in the left adrenal gland consistent with benign adenoma (arrow).,yes
PMC7440653,Figure_3,oa_package/a8/ff/PMC7440653.tar.gz,"['001) (A).', 'g003Expression level of TNC and immune response genes after ATN-RNA treatment in MDA-MB-231 cell line.', 'The highest concentration (100nM) used led to the dramatic drop of the protein expression measured as the 86% of the decrease (B and 3C).', 'The analysis was carried out with the qRT-PCR (A).', 'This enhancement in mRNA expression was 2 7-fold higher in poly I:C treated cells than in untreated cells (A, purple bars).']",10.1371/journal.pone.0237889.g003,yes
PMC7672125,Figure_5,oa_package/a5/d5/PMC7672125.tar.gz,"['The decalcified bone tissue around the round window as well as the round window niche and part of the tympanic scala were dissected and prepared for histopathology ().', 'Right cochlea from the beluga Qila.']",Figure 5 Right cochlea from the beluga Qila. General picture. Note there is multifocal congestion with hemorrhage at the round window (red arrow). Detail of the round window niche after being sampled for histopathology. Histopathology of focally extensive acute hemorrhage admixed with vacuolated proteinaceous deposits and dense clusters of extracellular and rare intracellular [arrow in ] Gram positive cocci. Sections were stained with hematoxylin-eosin and with Twort's gram stain.,yes
PMC7739559,Figure_11,oa_package/c2/96/PMC7739559.tar.gz,[],Fig. 11 UBM loss of substance at the level of the lesion,yes
PMC9517736,Figure_4,oa_package/7a/d3/PMC9517736.tar.gz,"['To further define these differences, chimeric replicons were created through the seamless exchange of Hypr and Vs sequences at the junction of NS3 or NS5, herein termed Hypr[NS3-Vs], Hypr[NS5-Vs], Vs[NS3-Hypr], and Vs[NS5-Hypr] (A).', 'In contrast, exchange of the NS3 coding region showed only a modest effect (B), with measurements at 24 hpt showing that the fluorescence associated with Vs Hypr-NS5 was as high as the parental Hypr strain, while that of Vs Hypr-NS3 showed no significant difference (', 'Plaque assay of supernatants from cells infected with WT viruses (24 hpi) showed similar patterns: Vs with Hypr NS5 showed a 10-fold increase in infectivity, while Hypr with Vs NS5 showed a significant attenuation (D).', 'These phenotypes were confirmed in Hypr and Vs viruses, validating the replicons for further chimeric assessments (D).', 'Through construction of the Hypr/Vs chimeras (), we found that the key determinant of the differential replication kinetics maps to the NS5 protein.']","FIG 4 Subcellular localization of WT and NS5 and NS3 chimeric TBEV replicons and viruses in PS cells. (A) Schematic representation of TBEV NS3 (Hypr[NS3-Vs], Vs[NS3-Hypr]), NS5 [Vs(NS5-Hypr) and Vs(NS3-Hypr)]) chimeras. (B) Replicon transfected cells were treated with DFHBI at 24 hpt and fixed in 4% PFA. Cells were imaged on an Airyscan microscope. Nuclei were stained with DAPI (blue). Scale bar: 10m. (C) Quantification of Spinach2-RNA expression of WT versus chimeric replicons in PS cells. Bar heights are the mean SD of two biological replicates. ****, < 0.0001 from WT. ns, no significant difference determined using a one-way ANOVA. (D) Quantification of plaque assays from PS cell supernatants transfected with the indicated TBEV replicons for 24 h. , < 0.01 compared to WT Hypr-IC determined using a one-way ANOVA.",yes
PMC4948237,Figure_3,oa_package/de/87/PMC4948237.tar.gz,"['The postoperative course was uneventful, and the rest of the lung expanded completely [].']",Figure 3 Postoperative chest X-ray showing expanded left lung,yes
PMC6360002,Figure_3,oa_package/93/26/PMC6360002.tar.gz,"['Chest radiography and CT-scan was done and ultrasonographic diagnosis was confirmed ().', '2The ultrasonography of the chest after the pneumothorax demonstrating the barcode sign (A) and lung point (B) the classic findings of the pneumothorax on ultrasonography (the arrow)The Supine chest radiography of the patient demonstrating the pleural catheter (arrow) in the left upper lung along with pneumothorax (A); axial chest CT-scan demonstrating the pneumothorax (arrow) after catheter insertion (B)']",Fig. 3 The Supine chest radiography of the patient demonstrating the pleural catheter (arrow) in the left upper lung along with pneumothorax (A); axial chest CT-scan demonstrating the pneumothorax (arrow) after catheter insertion (B),yes
PMC3987999,Figure_3,oa_package/9d/0d/PMC3987999.tar.gz,"['Dengue cases presented septum thickening with an increase of cellularity (b), the presence of mononuclear inflammatory infiltrates (', '3c) and hyperplasia of alveolar macrophages (g).', 'Cases 1 and 2, who had co-morbidities (diabetes and obesity), also showed hyaline membrane formation (e and 3f), probably due to dengue shock syndrome, with the concomitant hypertrophy of type II pneumocytes (', 'Alterations observed in cases 1 and 2 were evidenced with more detail in the ultrastructural analysis (k).', 'Virus-like particles were also detected in the endothelium of the lung in case 1 (l).', 'g003Histopathological and ultrastructural analysis of the lung.', 'Quantification of these damages showed larger areas of hemorrhage in cases 1 and 2 (m) and edema in cases 2 and 3 (', 'Isolated megakaryocytes and cell fragments with aspects of platelets were observed in alveolar space in semi-thin (i) and ultra-thin analysis (']",10.1371/journal.pone.0083386.g003,yes
PMC11342087,Figure_1,oa_package/70/39/PMC11342087.tar.gz,"['A brain CT scan () revealed a hypodense lesion located within the pons, without associated upstream hydrocephalus.', 'Axial section of a brain CT scan showing a round hypodense well circumscribed in the pons.', ':Sagittal (A), axial (B, D) and coronal (C) sections of a brain MRI on T2-WI (D) and T1-WI without contrast injection (A, B) and with contrast injection (C) showing a cystic lesion in the pons without any enhancement after injection of Gadolinium.']",Fig. 1 Axial section of a brain CT scan showing a round hypodense well circumscribed in the pons.,yes
PMC10660198,Figure_2,oa_package/64/79/PMC10660198.tar.gz,['Cardiac MRI heart morphology and function with and without contrast.'],"Figure 2 Cardiac MRI heart morphology and function with and without contrast. Single-shot bright blood acquisition without inversion pulse showed no signal of anterolateral papillary muscle, which is depicted here by the red arrows. Additionally, the anterolateral papillary muscle was hypointense compared with the normal myocardium on precontrast SSFP cine. On postcontrast dark blood delayed enhancement sequences, the papillary muscle complex was hyperintense compared with the normal myocardium. Taken together, our cardiologists reported that the patient had diffuse caseous toothpaste-like calcification of their anterolateral papillary muscle complex.",yes
PMC7513530,Figure_1,oa_package/c3/2b/PMC7513530.tar.gz,"[' 1a).', '1b).', '1c), indicating that the PD03 antibodies could cross the blood-brain barrier and accumulate in the brain.', 'Immunogenicity of PD03 in PLP- -syn mice, antibody binding in the brain, and gait analysis.', '01Nineteen weeks after the first injection, mouse motor performance was tested by Digigait analysis.', '1d).', '1d).', '1c).']","Fig. 1 Immunogenicity of PD03 in PLP--syn mice, antibody binding in the brain, and gait analysis. The plasma levels of antibodies binding to human -syn after PD03 immunotherapy reached a high level at week 6 and remained stable thereafter. ELISA-based competition assay demonstrating the competitive binding of PD03 antibodies to -syn monomers and fibrils. Percentage of area positive for IgG binding in the brains of vehicle- and PD03-treated PLP--syn mice (two-tailed -test, =8, =10, =6.742, *** 0.0001). DigiGait analysis of wild-type controls, and PLP--syn mice receiving vehicle or PD03. Data were analyzed with one-way ANOVA followed by Bonferronis test (* 0.05), ** < 0.01",yes
PMC4341019,Figure_3,oa_package/f6/b4/PMC4341019.tar.gz,"[' 3).', ' 3, Online Resource 3b) indicating that their epitope is located within the overlapping region of fragments I, II and III that consists of amino acids 49 148.', '', 'kDa Molecular weight (kilodalton)\niVHH detection of endogenous huntingtin in human HD brain lysatesNext, we analysed if our iVHH were capable of binding endogenous human htt.', 'eps"">Electronic supplemental figure 3.']","Fig.3 VHH epitope determination. Western blots performed on no htt (), htt a.a. 1148 Q46 (I), htt a.a. 15378 Q17 (II), and htt a.a. 49415 (III). Primary antibody indicated below each blot. Blots were performed twice. Molecular weight (kilodalton)",yes
PMC10453653,Figure_1,oa_package/62/b2/PMC10453653.tar.gz,"['Maximum intensity projection view of PET (A), transaxial views of PET (B), CT (C) and PET/CT fusion (D) images of the illustrative case.']","Figure 1 Maximum intensity projection view of PET ( ), transaxial views of PET ( ), CT ( ) and PET/CT fusion ( ) images of the illustrative case. A 47-year-old man with history of newly diagnosed left buccal poorly differentiated squamous cell carcinoma, who underwent PET/CT with F-fluorodeoxyglucose (FDG), was found to have a focal lesion (2.3 1.1 2 cm) with moderate increased FDG uptake (( , ) SUV 5.5) in the left buccal (( ) thick arrow). There were two foci with mild increased FDG uptake (SUV 2.3 and 1.3) in the left neck level I (( ) 0.94 cm; thin arrow) and level II (0.8 cm), respectively. Bilateral lungs were clear and no lesion was detected. Image staging was T2N1M0. Four days later, he received wide excision for a left buccal tumor and left modified radial neck dissection. Pathology reported a verrucous and ulcerated tumor measuring 4.1 2.7 1.3 cm. The modified radical neck dissection samples showed left neck level I one out of five metastatic squamous cell carcinoma, and left neck level II one out of one negative for malignancy. Pathologic staging was pT3N1. Later, he received concurrent chemoradiotherapy with 66Gy/33Fx plus concurrent cisplatin 6 cycles. Patients with a large metabolic tumor volume on FDG-PET may experience poor clinical courses [ ].",yes
PMC8590103,Figure_8,oa_package/68/eb/PMC8590103.tar.gz,[],"FIGURE 8 A model illustrating the differential responses of ApoE2 vs. ApoE4 carriers to mCRP and the differential regulation of mCRPinduced cerebrovascular neuroinflammation leading to AD pathogenesis in the brain. This study demonstrated a novel pathological mechanism, the competition of ApoE and mCRP to CD31binding, for cerebrovascular neuroinflammation resulting in an early stage of AD pathogenesis in the brain. During the chronic stage of peripheral inflammation, pCRP proteins disassociate into mCRP. mCRP binds to CD31 on bloodfacing endothelia to increase CD31 phosphorylation (pCD31), cause damage to the cerebrovasculature and induce extravasation of T lymphocytes into the brain, leading to AD pathogenesis (ApoE4>ApoE3>ApoE2). This process is antagonized by ApoECD31 binding (ApoE2>ApoE3>ApoE4) to block mCRPCD31 binding and differentially regulate pathways (mitochondrial function, epigenetics and vasculogenesis) to intervene in the neurodegenerative process of AD",yes
PMC9582872,Figure_6,oa_package/9a/5f/PMC9582872.tar.gz,['The most frequent treatment was ultra-potent topical corticosteroids (clobetasol propionate; n = 45; 72.'],Figure 6 Common vulvar intraepithelial neoplasia (VIN).,yes
PMC11495467,Figure_3,oa_package/1d/29/PMC11495467.tar.gz,"['\nFibulin-2 Expression in Meningiomas Seen in the Cytoplasm (200x magnification).', 'The highest Ki-67 group was low grade (40 cases (80%)) ().', 'In this study, Ki-67 expression ( and Table 3) showed a significant association with histopathologic grade (p = 0.']",Figure 3 Expression in Meningiomas Seen in the Cytoplasm (200x magnification). A. Low fibulin-2 expression (h-score 200). B. High fibulin-2 expression. Most tumors stained with high-intensity (h-score >200) (arrow).,yes
PMC11303169,Figure_2,oa_package/5b/fa/PMC11303169.tar.gz,"['Magnetic resonance imagingA multiparametric, non-contrast research MRI was performed at 3 T (Magnetom Prisma, Siemens Healthineers) at the Universities of Alberta and Calgary with a total scan time of approximately 75 min ().', 'Representative magnetic resonance images of multiple organs are shown for.']","Figure 2 Representative magnetic resonance images of multiple organs are shown for. Brain: Volumes with T1, lesions with FLAIR, white matter connectivity with DTI, and iron/myelin indication with QSM/R2* sequences. Lungs: Parenchyma lung water density quantification using free-breathing yarnball sequence. Heart: Structure, function from cine imaging and T1 and T2 mapping sequences. Body Composition: Abdominal fat/water separated imaging with chemical-shift encoded approach (multi-echo gradient echo sequence). Liver: PDFF, water-specific T1 and T2* using a SR-CSE sequence. Kidney: T1 mapping using the MOLLI sequence. Skeletal Muscle: PDFF, water-specific T1 and T2* with calculation of fat and muscle volumes and muscle T1.",yes
PMC8601975,Figure_1,oa_package/46/1e/PMC8601975.tar.gz,"['0 cm blur margin mass located at the right anterior-lateral wall of the urinary bladder ().', 'Computed tomographg (CT) scan shows the calcified right anterolateral mass lesion.', '', 'The CTU showed a mass occupying the same location of the anterior carcinoma ().']",Fig. 1 Computed tomographg (CT) scan shows the calcified right anterolateral mass lesion. A: First bladder malignancy (urothelial carcinoma); B: Second bladder malignancy (leiomyosarcoma).,yes
PMC7530220,Figure_4,oa_package/d2/0b/PMC7530220.tar.gz,"['Immunohistochemical staining was performed on the tumor cells resulting in Synaptophysin positive (), Chromogranin positive, and S100 highlights surrounding sustentacular cells.', '3DiscussionThere is little known about primary gallbladder paragangliomas.']",Fig. 4 (200) Positive labeling with Synaptophysin immunohistochemistry.,yes
PMC5474337,Figure_1,oa_package/a9/47/PMC5474337.tar.gz,['The distal space and depth of inclusion was determined by the Pell Gregory classification method.'],Figure 1 The distal space and depth of inclusion was determined by the Pell & Gregory classification method.,yes
PMC7600691,Figure_12,oa_package/8a/8a/PMC7600691.tar.gz,[],Figure 12 ( ) schematic images of the microfluidic chip with four identical stenosis regions. ( ) cross-section of the stenosis region. Adapted from Li et al. [ ].,yes
PMC5067885,Figure_1,oa_package/82/21/PMC5067885.tar.gz,"[' 1 and Additional file 1: S1).', ' 1 and Table 3, p = 0.', ' 1).', ' 1a and Table 4, p = 0.', ' 1a and Table 4, p 0.', ' 1b and Table 4, p 0.', ' 1e and Table 4, p 0.', ' 1f, Table 4, and Additional file 2: Table S1, SPSS statistical analysis).', 'Plasma biomarkers revealing significant association with the incidence of aMCI in ethnic cohorts.', 'aMCI amnestic mild cognitive impairment\nTable 3Statistical analysis of association between normal control and aMCI cohorts, independent of ethnicityBiomarker\np value, NC vs aMCIA 40\n 0.05). Moreover, for phospho-ERK1/2 protein expressed as a percentage of ERK1/2, the main effects of muscle type and genotype were significant (* = 0.0010, = 12.88 and = 0.0007, = 20.15, respectively). (E) Representative immunoblot analysis of the same set of leg muscle samples with the F4/80 macrophage marker to evaluate the abundance of macrophages in dystrophic muscles at 4 months of age.",yes
PMC3856422,Figure_7,oa_package/69/71/PMC3856422.tar.gz,"[' summarizes the relative light-penetration depth value for 60% glycerol (Gly), 60% PEG200, and the combination treatment of sonophoretic delivery (SP) with Gyl and PEG200/SP at 60 min measured with OCT.']","Figure 7 Changes in OCT imaging depth into in vivo optically cleared porcine skin with 60% glycerol, glycerol/, 60% PEG200, PEG200/at 1310 nm .",yes
PMC8167394,Figure_7,oa_package/1a/4b/PMC8167394.tar.gz,"['At one end of this spectrum is the so-called lipofibromatosis-like neural tumour (LLNT), composed of monomorphic spindle cells co-expressing S100 and CD34, featuring a highly infiltrative pattern within subcutaneous fat, therefore closely resembling lipofibromatosis (A).', 'A second subset of cases features a predominantly solid pattern of growth, and is composed of a cellular proliferation of uniform spindle cells that in higher grade examples resemble malignant peripheral nerve sheath tumours and therefore labelled as NTRK- rearranged neoplasm resembling peripheral nerve sheath tumours (B).', 'A.', 'B.']",Figure 3. . Anastomosing capillary-sized vessels are lined by hobnail endothelial cells in absence of significant nuclear atypia.,yes
PMC11551880,Figure_9,oa_package/34/32/PMC11551880.tar.gz,[],"FIGURE 9 Effect of NBDC on the expression of DRD1 and 5HT1AR. The protein expressions were determined by WB and IHC analysis. Protein bands and IHC results were quantified by densitometry. (A) Protein bands. (B) Protein expression results measured by WB. (C) DRD1 positive expression results ( 400). (D) 5HT1AR positive expression results ( 400). (E) Protein expression results measured by IHC. F: The HE staining hippocampus tissue micrographs ( 400). (a) Blank group, (b) model group, (c) lowdose group, (d) mediumdose group, (e) highdose group and (f) positive group. VS blank group, <0.05, <0.01; VS model group, * <0.05, <0.01.",yes
PMC10382898,Figure_9,oa_package/2e/a6/PMC10382898.tar.gz,"['11 If symptoms are not improved by injection or if symptoms localize to the supraclavicular area, targeted scalene injections (', ' 9Ultrasound-guided injection of local anesthetic targeted to the scalene triangle.']","Figure9 Ultrasound-guided injection of local anesthetic targeted to the scalene triangle. The white arrows indicate the needle. AS, anterior scalene; CA, carotid artery; IJ, internal jugular vein; MS, middle scalene; SCM, sternocleidomastoid.",yes
PMC10452064,Figure_2,oa_package/e4/75/PMC10452064.tar.gz,"['To move cytopathology into a virtual scenario, specifically from an educational point of view, the technical challenges and human adaptability should be taken into consideration ().', 'Equipped with virtual reality headsets, a clinician can visualize imaging and clinic data in an immersive fashion.']","Figure 2 Equipped with virtual reality headsets, a clinician can visualize imaging and clinic data in an immersive fashion.",yes
PMC8896839,Figure_1,oa_package/ac/35/PMC8896839.tar.gz,"['Morphological and immunohistochemical comparison of glioblastoma multiforme, WHO CNS grade 4, IDH wildtype and diffuse astrocytoma, WHO CNS grade 4, IDH mutant.']","Figure 1 Morphological and immunohistochemical comparison of glioblastoma multiforme, WHO CNS grade 4, IDH wildtype and diffuse astrocytoma, WHO CNS grade 4, IDH mutant. (A) Glioblastoma multiforme, WHO CNS grade 4 with pseudopalisadic necrosis (arrow), H&E stain, original magnification 80; (B) same tumor from (A), immunohistochemically negative for IDH R132H mutation, original magnification 200; (C) diffuse astrocytoma, WHO CNS grade 4 with glomeruloid neovascular proliferation (arrows), H&E stain, original magnification 200; (D) same tumor from (C), immunohistochemically positive for IDH R132H mutation, original magnification 200. WHO: World Health Organization; CNS: central nervous system; H&E: hematoxylin and eosin; IDH R132H: isocitrate dehydrogenase mutation variant.",yes
PMC4141340,Figure_17,oa_package/66/8b/PMC4141340.tar.gz,[],Fig. 17 Lymphogranuloma venerum in a 26-year-old HIV-positive man. Axial CT colonography demonstrates diffuse circumferential thickening of the rectum ( ) with inflammatory stranding in the mesorectum ( ). Reformatted virtual colonoscopy image demonstrates a smooth stricture ( ) with maintained mucosa. Sagittal T2-weighted MRI and Sagittal contrast-enhanced T1-weighted MRI reveal circumferential thickening ( ) with luminal compromise of the rectum which is hypointense on T2-weighted MRI and shows enhancement post gadolinium administration. Images in are the corresponding axial MR images showing similar findings,yes
PMC6212688,Figure_2,oa_package/43/72/PMC6212688.tar.gz,"['The size of other multiple metastatic lesions in the bilateral cerebral hemisphere, with or without hemorrhage, and the size of the right pons did not change ().', 'Postcontrast axial T1-weighted magnetic resonance finding of intracranial metastatic PCC at postoperation.']","Fig. 2 Postcontrast axial T1-weighted magnetic resonance finding of intracranial metastatic PCC at postoperation. A: Postoperative acute ischemic change and hemorrhage along the resection margin left parietal, temporal and frontal lobe. Slightly improved midline shift. B and C: No change in size of other multiple metastatic lesions with or without hemorrhage in the bilateral cerebral hemisphere and right pons.",yes
PMC8706756,Figure_4,oa_package/c1/ef/PMC8706756.tar.gz,"['As shown in A, CKD was induced by feeding 0.', 'As shown in B, the increased IS levels in plasma and renal tissues of the CKD mice was significantly suppressed by the AST-120 treatment, but not by the rapamycin treatment.', 'In addition, the activation of mTORC1 in the renal tissue of CKD mice was significantly suppressed by AST-120 or the rapamycin treatment (C).', 'In the renal tissues of CKD mice, the down-regulation of E-cadherin, up-regulation of -SMA and COL1A1 (D), and the up-regulation in the mRNA expression of inflammatory cytokines (TNF- and IL-6) (E) were observed, and these changes were significantly suppressed by the administration of AST-120 or rapamycin.', 'The fibrotic areas (staining with Picrosirius red) and increased hydroxyproline levels in the renal tissue in CKD mice were also significantly suppressed by the administration of AST-120 or rapamycin (F).', 'Therapeutic intervention targeting the IS/mTORC1 pathway in CKD mice.']","Figure 4 Therapeutic intervention targeting the IS/mTORC1 pathway in CKD mice. ( ) Experimental protocol for the effect of AST-120 or rapamycin on CKD mice. After randomization at 2 weeks after feeding a 0.2% adenine-containing diet, the AST-120 group was fed an 8% AST-120 containing diet and the rapamycin group received rapamycin (1 mg/kg) intraperitoneally daily. ( ) Effect of AST-120 or rapamycin on IS levels in the plasma and kidney of CKD mice. ( ) mTORC1 activity (pS6/S6) in the kidney of CKD mice. ( ) E-cadherin, -SMA, COL1A1, and -actin protein expressions in the kidney of CKD mice. ( ) TNF-, IL-6 mRNA expression in the kidney of CKD mice. ( ) Representative photomicrographs and quantification of Picrosirius red-stained kidney sections of CKD mice are shown. Original magnifications: 200. Scale bars represent 100 m. Hydroxyproline content in the kidney of CKD mice was measured. Data are expressed as the means SEM ( = 5), * < 0.05 compared with control in the absence of rapamycin; # < 0.05 compared with IS in the absence of rapamycin.",yes
PMC8675570,Figure_1,oa_package/a1/5b/PMC8675570.tar.gz,"['Coronal view: Left obstructing ureteropelvic junction calculi and bilateral hydronephrosisTransverse view: Left ureteropelvic junction calculiTransverse view: Right ureterovesical junction stoneWhile in the ED the patient progressively became hypotensive and was given broad-spectrum antibiotics, Vancomycin and Cefepime, due to concern for sepsis and aggressive fluid resuscitation was initiated.']",Figure 1 Coronal view: Left obstructing ureteropelvic junction calculi and bilateral hydronephrosis,yes
PMC8348376,Figure_8,oa_package/20/3b/PMC8348376.tar.gz,"['05) of the oligodendrocytes cultured from Tardbpfl/fl mice (, A C).', 'Simultaneous disruption in cholesterol biosynthesis and uptake machinery may underlie the demyelination phenotype caused by oligodendroglial TDP-43 deletion ( D).', '.', 'Reduced cholesterol metabolism related gene expressions in the glia from postmortem FTD patientsTo further extend our findings to human settings, we colabeled TDP-43, HMGCS, and LDLR with oligodendrocyte marker (CC1) using human spinal organoid derived from induced pluripotent stem cells (iPSCs; A).', 'Simultaneous disruptions in cholesterol biosynthesis and uptake, two molecular mechanisms required for all animal cells to maintain cholesterol homeostasis, are likely one of the causes of the demyelination phenotype observed in oligodendroglial TDP-43 deletion mice ( D; Wang et al.']","Figure S4. Domain organization of human SREBF2 protein. Antibody epitope is within 455469 aa. Cell lysates from (i) siRNA-control and siRNA-SREBF2, (ii) control plasmid and full-length cDNA expressing SREBF2. Black arrow: full-length SREBF2; blue arrow: N-terminal processed transcription domain. Domain organization of human LDLR protein. Antibodies were produced using recombinant protein within 1350 aa (for Proteintech, 107851-AP) or 500550 aa (Novus, NBP1-06709-SS) for LDLR. Cell lysates from (i and iii) siRNA-control and siRNA-LDLR, and (ii and iv) control plasmid and full-length cDNA expressing GFP-LDLR. Black arrow: endogenous LDLR; green arrow: GFP-LDLR. Domain organization of human HMGCS1 protein. Antibody was produced using recombinant protein within 171520 aa. Cell lysates from (i) siRNA-control and siRNA-HMGCS1, and (ii) control plasmid and full-length cDNA expressing HMGCS1. Black arrow: endogenous HMGCS1. Domain organization of human HMGCR protein. Cell lysates from (i) siRNA-control and siRNA-HMGCR, and (ii) control plasmid and full-length cDNA expressing HMGCR. Black arrow: endogenous HMGCR. ctrl, control.",yes
PMC11632728,Figure_1,oa_package/e3/e3/PMC11632728.tar.gz,"['\nThe most lateral point of the mandibular condyle (O1), the most lateral point of the mandibular ramus (O2), and the most superior point of the condyle.']","Figure 1 The most lateral point of the mandibular condyle (O1), the most lateral point of the mandibular ramus (O2), and the most superior point of the condyle.",yes
PMC4183529,Figure_4,oa_package/70/31/PMC4183529.tar.gz,"['U0126 treatment reduced the enhanced neutrophil infiltration in Spred-2 / mice (A).', 'Likewise, U0126 dramatically ameliorated the exacerbated LPS-induced lung pathology observed in Spred-2 / mice (B).', 'The cytokine and chemokine response at 24 hours was next investigated in Spred-2 / mice, and we found that U0126 significantly reduced the increased TNF- , CXCL2 and CCL2 levels, but not IL-10, in Spred-2 / mice (C).', 'g004U0126 inhibits the enhanced lung inflammation in Spred-2 / mice.']",10.1371/journal.pone.0108914.g004,yes
PMC6233031,Figure_2,oa_package/db/d1/PMC6233031.tar.gz,"['Other atypical features seen were pustules in 7%, pseudoimbricata 1% [], arciform lesions in 3%, and a case of erythroderma.']",Figure 2 Tinea pseudoimbricata - multiple concentric circles with intermittent areas of clearing seen in steroid modified tinea,yes
PMC8451510,Figure_2,oa_package/ac/24/PMC8451510.tar.gz,['Digital subtraction pulmonary angiography demonstrates the peripheral pulmonary artery aneurysm (red arrow) arising from the superior segmental and subsegmental branches (yellow arrow).'],Figure 2 Digital subtraction pulmonary angiography demonstrates the peripheral pulmonary artery aneurysm (red arrow) arising from the superior segmental and subsegmental branches (yellow arrow). A pigtail catheter is seen in the left pulmonary artery (blue arrow). The lower lobe pulmonary artery is also seen (green arrow).,yes
PMC4670855,Figure_3,oa_package/83/12/PMC4670855.tar.gz,['Elimination of RAGE does not affect total white blood cell and blood neutrophil counts following intestinal IR.'],"Figure 3 . Mice were subjected to 30min mesenteric artery occlusion, followed by 150min reperfusion. The numbers of white blood cells (WBC) and neutrophils, in the peripheral blood collected after 150min reperfusion, demonstrating that RAGE does not influence IR-induced leukocyte or neutrophil mobilization. Data are presented as meanSEM, =5 (WT-SHAM); =13 (WT-IR); =15 (RAGE IR) where * <0.05, ** <0.01, and ns=not-significant ( >0.05).",yes
PMC7415277,Figure_2,oa_package/87/c6/PMC7415277.tar.gz,"['Sonographic examination using linear multifrequency probe revealed a right retroareolar cyst with maximum diameter of 12 mm 11 mm containing turbid echogenicity with surrounding hyperemia [].', 'Sonographic examination using linear multifrequency probe revealed a right retroareolar cyst with maximum diameter of 12 mm 11 mm containing turbid echogenicity with surrounding hyperemiaDiscussionAwareness of breast malignancy increases with years and with any changes in the breast comes more stress.']",Figure 2 Sonographic examination using linear multifrequency probe revealed a right retroareolar cyst with maximum diameter of 12 mm 11 mm containing turbid echogenicity with surrounding hyperemia,yes
PMC4961442,Figure_5,oa_package/a4/30/PMC4961442.tar.gz,"['Compared to WT and AICD animals, hTau and AICD;hTau animals displayed higher levels of somato-dendritic accumulation of phosphorylated-tau in the cerebral cortex (A) and in the hippocampus (B).', 'In addition, overexpression of human tau increased p-NMDAR/total NMDAR levels in AICD transgenic mice (C and 5D).', 'Interestingly, overexpression of human tau alone was sufficient to increase p-NMDAR levels in WT mice (C and 5D).', 'g005Increased somato-dendritic tau accumulation and phosphorylation of NMDAR after hTau overexpression.']",10.1371/journal.pone.0159435.g005,yes
PMC8389935,Figure_2,oa_package/2d/cd/PMC8389935.tar.gz,"['HE and IHC images of this tumor, (A) epithelioid cell type (HE, 400), (B) IHC staining of CD117 positive (IHC, 400), (C) IHC staining of DOG-1 positive (IHC, 100), (D) IHC staining of SMA positive (IHC, 100), (E) IHC staining of Ki-67 proliferation index (IHC, 100).']","Figure 2 HE and IHC images of this tumor, (A) epithelioid cell type (HE, 400), (B) IHC staining of CD117 positive (IHC, 400), (C) IHC staining of DOG-1 positive (IHC, 100), (D) IHC staining of SMA positive (IHC, 100), (E) IHC staining of Ki-67 proliferation index (IHC, 100). HE = hematoxylin-eosin staining, IHC = immunohistochemistry.",yes
PMC10601793,Figure_3,oa_package/40/da/PMC10601793.tar.gz,['.'],"Fig. 3. AN in abdominal skinfolds, left-sided view.",yes
PMC7431516,Figure_28,oa_package/f4/ae/PMC7431516.tar.gz,[],"Fig. 28 A 70-year-old female with diabetes and Charcot foot. MRI showed extensive bone marrow edema (dashed arrows) in all bones of the foot suggesting active Charcot changes, most prominent at the level of the Lisfranc joint, where dorsal dislocation of os cuneiforme intermedium is visible (arrow)",yes
PMC6420333,Figure_1,oa_package/33/ed/PMC6420333.tar.gz,['Comparison of scout film and thoracic computed tomography(A) Scout film demonstrating multiple left-sided rib fractures (arrows).'],Figure 1 Comparison of scout film and thoracic computed tomography (A) Scout film demonstrating multiple left-sided rib fractures (arrows). (B) Coronal chest computed tomography (CT) images confirm the presence of multiple left-sided rib fractures (arrows).,yes
PMC5529650,Figure_12,oa_package/e5/07/PMC5529650.tar.gz,[],"Figure 12 NOX2 deletion significantly attenuates TBI-induced TXNIP expression and complex formation within the injured mouse cortex. (a) Expression of TXNIP in the injured cortex after TBI. Representative images from sham, WT, and NOX2 KO mice show that TBI increases expression of TXNIP in the injured cortex after TBI, and TXNIP colocalizes with NLRP3 expression. Deletion of NOX2 attenuates the expression of TXNIP in the injured cortex at the 4-day post-TBI. Immunoreactivity from all images has been quantified to the right of the representative panel (data presented as fold change relative to shams). =4, 6, and 6 mice (sham, WT, and KO, resp.). (b) In situ PLA demonstrating NLRP3-TXNIP complex formation after TBI and regulation by NOX2. Representative confocal images of Duolink in situ co-IP show red fluorescence indicative of NLRP3-TXNIP protein-protein interaction in the injured cortex at the 4-day post-TBI. Mice deficient in NOX2 show reduced NLRP3-TXNIP complex formation at the 4-day post-TBI in the injured cortex. Quantification of all Duolink images shows significantly increased NLRP3-TXNIP interaction after TBI that is attenuated by NOX2 deletion. =4, 6, and 6 mice (sham, WT, and KO, resp.). Scale bar represents 50 M. Data for (a) and (b) presented as fold change relative to sham mice. ( < 0.05, < 0.001 sham versus WT TBI; < 0.05, < 0.0001 WT TBI versus KO TBI).",yes
PMC10672606,Figure_2,oa_package/44/f2/PMC10672606.tar.gz,"['The imbalance of Tau kinase and phosphatase activities induces and accelerates the production of the P-Tau protein, which self-aggregates into many paired helical filaments (PHF) and further competes for binding to form NFTs, ultimately leading to microtubule destabilization and impaired neuronal transport ().', 'The formation of NFT: In the presence of GSK3 and CDK5, the Tau protein is hyperphosphorylated into P-Tau.']","Figure 2 The formation of NFT: In the presence of GSK3 and CDK5, the Tau protein is hyperphosphorylated into P-Tau. After microtubule disassembly, the P-Tau loses its binding to microtubules and self-aggregates into multiple pairs of helical filaments (PHF), further forming neurofibrillary tangles (NFT).",yes
PMC11095255,Figure_5,oa_package/65/60/PMC11095255.tar.gz,"['An Amplatzer vascular plug (Abbott Vascular, Santa Clara, USA) was placed in the splenic vein, extending distally to just surpass the inferior mesenteric vein (IMV) insertion ().', 'This allowed for the exclusion of the splenic vein segment that lies between the inferior mesenteric vein insertion point and the spleno-mesenteric confluence ().', 'CT scan after other interventional radiology with plug in the splenic vein and cover stent in the superior mesenteric vein.']","Figure 5 CT scan after other interventional radiology with plug in the splenic vein and cover stent in the superior mesenteric vein. PV portal vein, SMV superior mesenteric vein, SV splenic vein, IMV inferior mesenteric vein.",yes
PMC8496098,Figure_2,oa_package/2a/f7/PMC8496098.tar.gz,"['The remaining three patients with sBBMI and false negative BIPS were a case with mesenteric laceration but without mesenteric haematoma 5 cm on CT (), and two cases with active mesenteric bleeding, but negative physiological findings (i.', 'Coronal (a) and axial (b) iodinated contrast-enhanced CT images acquired in a 54-year-old woman after a traffic road accident show haemoperitoneum without solid organ laceration (a, arrowhead), non-focal jejunal bowel wall thickening (a, arrows), and mesenteric vascular extravasation (b, arrow), suggesting sBBMI.', 'Among the 732 patients without sBBMI, the Faget score was false positive in 66 patients (9%) and the BIPS in 74 patients (10.']","Fig. 2 Coronal (a) and axial (b) iodinated contrast-enhanced CT images acquired in a 54-year-old woman after a traffic road accident show haemoperitoneum without solid organ laceration (a, arrowhead), non-focal jejunal bowel wall thickening (a, arrows), and mesenteric vascular extravasation (b, arrow), suggesting sBBMI. This was confirmed by immediate laparotomy (mesenteric suture, small bowel suture). McNutt and Faget scores were 1 (negative) and 10 points (positive), respectively.",yes
PMC2938495,Figure_11,oa_package/1b/22/PMC2938495.tar.gz,[],Figure 11 The apical 4-chamber view of a 5-year-old child with repaired Hypoplastic Left Heart Syndrome. Note the extremely small size of the left ventricle (LV) in relation to the right ventricle (RV),yes
PMC9663720,Figure_3,oa_package/31/5e/PMC9663720.tar.gz,"[' 3b RBV at baseline in grayscale) without requiring contrast agents (microbubbles).', ' 3a).', ' 3b).', ' 3c).', 'Neurovascular coupling in the Retinal Vascular Network (a) Doppler image of the rat eye.', 'An RBV profile was extracted using the newly defined region of interest.', ' 3d).', ' 3e).']",Figure 3 Neurovascular coupling in the Retinal Vascular Network ( ) Doppler image of the rat eye. ( ) Zscore activation map of the retina. ( ) Delta RBV Map is showing activation percentage compared to baseline for each pixel. ( ) Activation Map-based ROI ( <0.05). ( ) ROI Mean RBV temporal Signal over the total acquisition (1660s) comprised 50 trials. Each peak is retinal hyperemia. ( ) Microvasculature of the retina by ULM ( ) Zoom in the RBV temporal Signal allows better visualization of single-trial responses. The visual stimulation pattern is represented in red. ( ) Mean trial response over the 50 trials with SEM. The visual stimulation pattern is represented in red.,yes
PMC11408156,Figure_8,oa_package/67/06/PMC11408156.tar.gz,"['8Semaglutide and tirzepatide do not reduce hippocampal microglial activation or astrogliosis in 6-month-old 5XFAD miceThe extent of microglial activation and reactive astrocytes, as measured by the density of IBA1- and GFAP-positive cells, respectively, were both elevated in the hippocampus ( 8A E) and subiculum (Supplementary 8A-D) of 6-month-old male and female 5XFAD mice compared to WT littermates.', 'However, neither semaglutide nor tirzepatide altered IBA1 or GFAP cell density in either the hippocampus or subiculum of 6-month-old male and female 5XFAD mice ( 8A E, Supplementary 8A-D).', ' 8Semaglutide and tirzepatide do not reduce the number of activated microglia or reactive astroglia, or attenuate dysregulated gene expression related to neurodegeneration in the hippocampus of 6-month-old 5XFAD mice.', 'Levels of mRNA transcripts encoding Triggering Receptor Expressed on Myeloid cell-2 (TREM2), a key regulator that switches myeloid cells from a homeostatic to a neurodegenerative phenotype [29], were upregulated in the hippocampus of male and female 5XFAD mice, but not altered by treatment with semaglutide or tirzepatide ( 8F).', 'However, Tyrobp mRNA levels trended lower in tirzepatide-treated female mice ( 8G).', 'Nevertheless, for the majority of mRNA transcripts examined, the expression of genes encoding biomarkers of inflammation such as Clec7a, Cd68, Ccl2, Cxcl10, Gfap, Il1b, Tnf, and Il6 were increased in the hippocampus of 5XFAD vs WT control mice, but not different after treatment with semaglutide or tirzepatide ( 8HM, Supplementary 8E-F).']","Figure7 (AD) Locomotory activity was evaluated by an Open Field test in 6-month-old female (, A) and male (, B) 5XFAD mice treated with semaglutide (25nmol/kg), tirzepatide (10nmol/kg), or vehicle (saline). The percentage of time spent in the center area of the arena for female (C) and male (D) 5XFAD mice. (EH) tests were performed to assess exploratory behavior and recognition memory. The percentage of exploration time spent on the novel object for 6-month-old female (E) and male (F) 5XFAD mice treated with semaglutide (25nmol/kg), tirzepatide (10nmol/kg), or vehicle (saline). The discrimination index, defined by the difference of the time spent exploring the novel object over the total object exploration time, of female (G) and male (H) 5XFAD mice. (IJ) . Daily latency time to reach the platform during the 4-day trial acquisition task (training) of 6-month-old female (I) and male (J) 5XFAD mice treated with semaglutide (25nmol/kg), tirzepatide (10nmol/kg), or vehicle (saline). (KN) Latency to the target quadrant (K, L) and number of crossings on the target quadrant (M, N) on Probe test day (Day 5) of 6-month-old female (K, M) and male (L, N) 5XFAD mice treated with semaglutide (25nmol/kg), tirzepatide (10nmol/kg), or vehicle (saline). Data for (A, B, I, J) were analyzed by 2-way ANOVA with Sidak's post-hoc test, and for (CH, KN) by MannWhitney test, or unpaired, two-tailed Student's t test for comparison between vehicle- and semaglutide- or tirzepatide-treated mice. <0.05, <0.01 vehicle vs. treatment. Data are represented as meansSD (A-F, IN), or as box-and-whisker plots (G, H) of female () and male () mice as indicated. Dashed lines in panels IJ indicate the time range (longest and shortest times) required to reach the platform over the 4-day training period. n=610 in each group of vehicle (Veh)-, semaglutide (Sema)-, or tirzepatide (TZP)-treated mice.",yes
PMC7463502,Figure_5,oa_package/ca/50/PMC7463502.tar.gz,"['SkQ1 Prevents Accumulation of Proteins p38 MAPK and p-p38 MAPK in the Hippocampus of OXYS RatsSupplementation with SkQ1 had no effect on the p38 MAPK content in the hippocampus of OXYS rats but significantly changed the level of phosphorylation of p38 MAPK in both protein fractions (a c).', 'In the detergent-insoluble fraction, we noted a similar decrease in p-p38 MAPK content, but here, the level of phosphorylation in OXYS rats taking SkQ1 did not reach the level of Wistar rats (a c).', 'The western blotting data were supported by immunostaining findings (d).', '02; a b), and dietary supplementation with SkQ1 significantly reduced this level in OXYS rats (p 0.', '01; a b).', 'In the detergent-insoluble fractions, the MK2 protein was absent in both rat strains (a,c).', '02; a,b) and detergent-insoluble fractions (p 0.', '02; a,c).', '01, respectively; a c).', 'MK2 and p-MK2 amounts were lower in the hippocampus of the OXYS rats taking SkQ1 than in untreated OXYS rats ().', 'The impact of SkQ1 supplementation in OXYS rats from age 12 to 18 months on the amounts of proteins p38 MAPK, p-p38 MAPK, MK2, and p-MK2 in the hippocampus.']","Figure 5 The impact of SkQ1 supplementation in OXYS rats from age 12 to 18 months on the amounts of proteins p38 MAPK, p-p38 MAPK, MK2, and p-MK2 in the hippocampus. Representative western blots of total and phosphorylated p38 MAPK and MK2 in the detergent-soluble and detergent-insoluble fractions in the hippocampus of untreated Wistar rats and OXYS, and OXYS rats with SkQ1 ( ). Graphical presentation depicts relative protein content of p38 MAPK, p-p38 MAPK, MK2, and p-MK2 in the hippocampi of untreated Wistar and OXYS rats and in OXYS rats taking SkQ1 after normalization to -actin for the detergent-soluble protein fraction ( ) and normalization to GAPDH for the detergent-insoluble fraction ( ). Data are presented as mean SEM of five independent experiments. Immunostaining of p38 MAPK and p-p38 MAPK ( ) in the hippocampus of untreated Wistar and OXYS rats and OXYS rats taking SkQ1. The nuclei were stained with DAPI (blue). Scale bars, 25 m. ^ Statistically significant differences between the strains of the same age; * the effect of supplementation with SkQ1 ( < 0.05).",yes
PMC10592772,Figure_5,oa_package/a7/b7/PMC10592772.tar.gz,"['braziliensis-infected Rag1 / mice were reconstituted with WT or CCR5 / CD8+ T cells, and the course of infection was monitored (A).', 'Importantly, Rag1 / mice reconstituted with CCR5 / CD8+ T cells (CCR5 / CD8) exhibited significantly smaller lesions (B) with less pathology (C and 5D).', 'In contrast, parasite burdens were similar in Rag1 / mice that received WT or CCR5 / CD8+ T cells (E).', 'Additionally, Rag1 / + CCR5 / CD8 mice had a significant reduction in the frequency and number of neutrophils (CD11b+ Ly6G+ cells) (I K) and in neutrophils expressing pro-IL-1 (L N).', '561700v1-f0004"" position=""float""/>.']","Fig 5. CD8 T cell migration to the lesion is CCR5-dependent. (A) Rag1 mice were infected with and reconstituted with WT or CCR5 CD8 T cells. (B) Ear thickness and (C) pathology score was assessed weekly. (D) Representative pictures of lesions from infected-Rag1 that received WT CD8 or CCR5 CD8 T cells at 5 weeks post-infection. (E-N) At 5 weeks post-infection, mice were euthanized, and the ears were digested for (E) parasite quantification by limiting dilution and (F-N) flow cytometry analysis. (F) Representative dot plots and graph bars of the (G) frequency and (H) number of CD8 T cells. (I-N) The neutrophil number (CD11b Ly6G cells) and pro-IL-1 expression in the lesion were determined directly by flow cytometry. (I-K) Representative dot plots and graph bars of neutrophils and (L-N) pro-IL-1 expression. The data are expressed as the means SEMs and are representative of two independent experiments (n = 35 mice/group). < <",yes
PMC9441100,Figure_4,oa_package/cd/d4/PMC9441100.tar.gz,"[' 4a).', ' 4a arrowheads, c).', ' 4b).', ' 4a arrows, d).', ' 4a, e), highlighting the impact of co-morbid LATE-NC on pTau pathology and consequently on GVD-mediated necroptosis activation.', 'pMLKL and pTau severity as well as neuronal loss are increased in AD cases with LATE-NC.', 'key=475a3bbe-6fc9-476e-8e45-6429422b85bfFinally, we performed semi-partial correlation analysis controlled for sex but not for age (as age distributions among groups do not overlap, Additional file 1: A1b).', ' 4f).', ' 4f).']","Fig. 4 pMLKL and pTau severity as well as neuronal loss are increased in AD cases with LATE-NC. Local accumulation of phosphorylated tau and MLKL is observed in AD . DAB immunohistochemical staining of pTDP-43 (S409/S410), pTau (S202/T205) and pMLKL (S358) in the CA1-subiculum field of a control, AD and AD case (cases 7, 16 and 21 are displayed, see Table ). Scale bars=50m. AD cases display decreased neuronal density compared to controls and AD and controls. Quantitative data representing the number of total neurons per mm per group in the CA1 subfield of the hippocampus. Quantification of the number of CA1 positive neurons for pTDP-43 pMLKL and pTau. AD cases display significantly higher severity of pMLKL and pTau lesions. Data are presented as meanSEM. N=27 (controls=9, AD =8, AD =10). Partial Spearman correlation matrix controlled for age and multiple comparisons (Holm-Bonferroni test) in this cohort confirms that the accumulation of pTDP-43, pMLKL and pTau pathologies are significantly correlated with neuronal density in the CA1, AMTL phase and Braak NFT stage. Supporting images showing overviews of the whole hippocampus of cases 12, 16, 21 and 22 (Table ) stained with pMLKL can be found in the public repository BioImage Archive, with the hyperlink:",yes
PMC11339419,Figure_1,oa_package/3a/a0/PMC11339419.tar.gz,"[' 1 illustrates the concept of our proposed PA ablation lesion indexing, highlighting the ablation-induced lesion boundary during catheter RF ablation procedures.', 'The idea of photoacoustic (PA) ablation lesion indexing: detection ablation-induced lesion boundary during catheter RF ablation procedures.', 'MethodThe proposed lesion boundary detection based on PA comprises three steps outlined in ']",Figure 1 The idea of photoacoustic (PA) ablation lesion indexing: detection ablation-induced lesion boundary during catheter RF ablation procedures.,yes
PMC10634831,Figure_1,oa_package/ac/92/PMC10634831.tar.gz,"['ResultsThe early ENS macrophage response limits the spread of enteric synucleinopathyTo assess the potential role for the gut-resident immune system in modulating local or distal synucleinopathy, we employed a recently described gut-first model of -synuclein ( -syn) pathology17 by injecting preformed fibrils of mouse recombinant -syn directly into the stomach and duodenal wall of wild type mice (A).', 'We first assessed the time course for local seeding of -syn pathology within the enteric nervous system using whole mount immunofluorescent microscopy at 4, 14, and 30 days post injection (dpi), and found that enteric synucleinopathy was first observed within the myenteric plexus of pre-formed fibril (PFF)-injected mice at 14dpi, and further increased through 30dpi and 60dpi (B,C).', 'Interestingly, we noticed that macrophages near diseased ganglia directly contacted pSer129+ neurons and contained internalized pSer129+ punctae (D).', 'These punctae and the contact between macrophages and neurons was increased in our PFF mice without changes in total macrophage area (E G, and Fig S1C,D).', 'To address this, we depleted peripheral macrophages using injections of a monoclonal antibody against CSF1R24, which is required for macrophage survival, without depleting central microglia (H, Fig SG, H).', 'Interestingly, loss of ENS macrophages further enhanced the ENS phospho- syn pathology induced by PFF injection at 30dpi (I, J).', 'Consistent with our previous analysis (F,G), there was a positive correlation between the neuronal pSer129 -syn+ pathology load and the amount of neuron-macrophage contact that was unaffected by loss of C1q (K), suggesting that chemotactic signaling remained intact.', 'Given that our data demonstrated further progression of pSer129 -syn+ pathology from 30 to 60dpi (B, C), we asked if this macrophage response was sustainable or if it eventually failed.', '17052351:Myenteric macrophages modulate enteric synucleinopathy.']","Figure 1: Myenteric macrophages modulate enteric synucleinopathy. (A) Cartoon depicting gut wall injections of PFFs. (B) Representative confocal images of the myenteric plexus (Tuj1, pseudo colored magenta) and pSer129 -synuclein (pseudo colored yellow) at 4, 14, and 30 days post injection (dpi) with either saline or PFFs. (C) Counts of the average number of pSer129+ neuronal soma/ganglia within the myenteric plexus shows PFF but not saline injection induces neuronal pSer129 pathology starting at 14dpi, with a time-dependent increase to 30dpi. Two-way ANOVA with Tukeys multiple comparison test: interaction p<0.0001, injection p<0.0001, time p<0.0001; n=45 animals/group/time point. (D) Representative confocal image of myenteric plexus (Tuj1, pseudo colored magenta), myenteric macrophages (MHCII, pseudo colored cyan), and pSer129 -synuclein (pseudo colored yellow) from 30dpi PFF mice depicting a macrophage contacting a pSer129+ soma (left) and a macrophage with internalized pSer129+ punctae (right). (E) Quantification of the average pSer129+ punctae size in myenteric macrophages at 30dpi showing a statistically significant increase in PFF-injected mice. Unpaired t-test, p<0.05; n=5 animals/group. (F) Thresholded and binarized confocal images depicting the contact sites (white) between the myenteric plexus (magenta) and myenteric macrophages (cyan) at 30dpi. (G) Quantification of the average macrophage-neuron contact site size. Unpaired t-test, n=5 animals/group. (H) Cartoon of design for macrophage depletion experiments. (I) Representative confocal images of myenteric ganglia (Peripherin, pseudo colored magenta) with pSer129 -synuclein pathology (pseudo colored yellow) at 30dpi. (J) Counts of average number of pSer129+ neuronal soma per myenteric ganglia replicating that PFF injection induces neuronal pSer129+ -synuclein pathology at 30dpi and additionally showing macrophage depletion with PFF injection further increases neuronal pSer129 -synuclein burden. One-way ANOVA (p<0.0001) with Sidaks test for multiple comparisons; n=35 animals/group.",yes
PMC4631791,Figure_5,oa_package/07/47/PMC4631791.tar.gz,['.'],"Fig. 5. A collection of CA pneumonia isolates ( =31) was analysed for toxin levels and cytotoxic activity in bacterial culture supernatants. (A) Correlation of cytotoxic activity in bacterial culture supernatants towards lung epithelial cells versus -toxin concentrations (left -axis; open symbols) and neutrophils versus PVL levels (right -axis; filled symbols). (B,C) Live imaging analysis of lung tissue models exposed for 16h to culture supernatants from CA pneumonia strains ( =18, selected to obtain a representative collection of supernatants with varying -toxin and PVL levels). The graphs show the correlation between epithelial injury (intensity sum) and levels of -toxin (B) and PVL (C) in the culture supernatants. (D) Comparison between percentage cytotoxicity elicited towards lung epithelial cells and neutrophils (PMN) by strains isolated from non-survivors (NS) and survivors (S). (E) Strains were divided as eliciting high (>30%, black bars) or low (<30%, white bars) cytotoxicity. (F) -toxin and PVL levels in bacterial supernatants of strains isolated from survivors (S) versus non-survivors (N.S). Pearson's correlation test was used in A-C, and and values are indicated. Statistical differences between N.S and S were determined using the MannWhitney test in D and F, and Fisher's exact test in E; * <0.05.",yes
PMC3856422,Figure_15,oa_package/69/71/PMC3856422.tar.gz,[],Figure 15 (online color at: ) Typical results obtained from 3DISCO of neurons in the brain and hippocampus. (a) 3D reconstruction of an entire mouse brain with a corner-cut view; (bd) The horizontal projections from the cleared brain at different depths marked in a. (e) 3D reconstruction of a cleared hippocampus with a corner-cut view showing some of the neurons that reside in the tissue; (f) The horizontal projection from the cleared hippocampus at the indicated plane in e. (g) Higher magnification of the region indicated in f .,yes
PMC6775334,Figure_4,oa_package/43/73/PMC6775334.tar.gz,['SA of the left breast in a 42-yr-old woman.'],"Figure 4 SA of the left breast in a 42-yr-old woman. On US, the SA lesion appears a round low echo nodule (about 15mm 11mm in size) with ill-defined margin and heterogenous echo, and a sound shadow can be found behind the lesion; and no obvious blood flow was found on color Doppler ultrasound. SA=sclerosing adenosis, US=ultrasound.",yes
PMC4864133,Figure_2,oa_package/2f/ae/PMC4864133.tar.gz,['Examples of some of the neuropathological abnormalities in SUDEP.'],"Figure 2 Examples of some of the neuropathological abnormalities in . ( ) Evidence of brain swelling in with gyral flattening over convexities in a 39yearold female. ( ) Bilateral occipital ulegyric malformation following a focal perinatal ischaemic event, simulating polymicrogyria, in a 52yearold male with . ( ) Acute eosinophilic neuronal change in the 1 sector with scattered pyramidal neurones showing this alteration in (arrow) ( ). An individual with a clinical diagnosis of DiGeorge syndrome showing an impression of exaggerated microcolumnar cytoarchitecture (arrows showing columns of >10 neurones and loss of horizontal lamination) in the middle temporal lobe gyrus; also in this case, bilateral hippocampal atrophy was due to hippocampal sclerosis ( ) with an impression of incomplete hippocampal inversion with an upward pointing hilus. Bar is equivalent to 40 microns approximately in ( ) and 250 microns in ( ).",yes
PMC4572858,Figure_6,oa_package/16/c9/PMC4572858.tar.gz,"['The identity of each recorded cell and its pattern of dendritic\narborization in the inner plexiform layer was confirmed post hoc\nfollowing injection of Alexa Fluor 594 through the recording pipette\n(s 6a e).', 'Analysis of RGC\nintrinsic properties, including membrane resting potential and resistance,\ndid not reveal significant differences between intact and axotomized neurons\nwith or without siREDD2 treatment (s 6f and\ng).', '; s 6h and i).', 'Remarkably, siREDD2 treatment restored the light-induced firing frequency in\naxotomized RGCs to levels similar to those recorded from intact neurons\n(axotomy siREDD2: 22 4 Hz; s 6j\nand k and Table 1).', 'No significant\nchange was observed in the amplitude of action potentials (l).', 'org/1999/xlink"" xlink:href=""cdd2014149f5""/>siREDD2-mediated mTOR activation restores RGC function.']","Figure 6 siREDD2-mediated mTOR activation restores RGC function.( ) Whole-cell recordings were obtained fromON-center YFP-positive RGCs visualized with epifluorescence and infrareddifferential interference contrast (DIC) optics to position the recordingelectrode. ( , and ) The identity of each recordedcell was confirmed following injection of Alexa Fluor 594 through therecording pipette. Analysis of RGC intrinsic properties, including membraneresting potential ( ) and resistance ( ), did not revealsignificant differences between intact and axotomized neurons with orwithout siREDD2 treatment. ( and ) Light stimulationdemonstrated an increase in the frequency of action potentials elicited byaxotomized RGCs ( =7 cells) compared with non-injuredcontrols ( =7 cells). ( and ) siREDD2treatment restored the light-induced firing frequency to levels similar tothose recorded from intact neurons ( =7 cells). ( )No change in the amplitude of action potentials was observed. Values areexpressed as the meanS.E.M. (ANOVA,*** <0.001). Cells were recorded from 5 to 7 miceper group. Scale bars:( )=20 m",yes
PMC8467069,Figure_5,oa_package/f4/31/PMC8467069.tar.gz,"['mDA neuronal culture from a healthy line displayed normal mitochondrial morphology arranged in a tubular network (A arrow).', 'In contrast, many of the cells in fPD-derived mDA neuronal cultures manifested clump-like immunostainings (A arrowhead), indicating abnormal mitochondrial morphology.', 'Importantly, we also noted that clumping of mitochondrial staining tends to associate with accumulated -syn in these cells (A).', 'Image analysis indicated that a significantly higher proportion of cells in the A53T line suffered from abnormal mitochondrial morphology compared to the healthy control (B).', 'The mDA neuron culture from the SNCA triplication line also displayed elevated levels of abnormal mitochondrial morphology (B).', 'The magnitude of this difference was small but significant (C).', 'CCCP treatment reduced cellular survival of DA neuronal cultures from all lines tested in a dose-dependent manner (C).', 'However, the effect of CCCP treatment was significantly higher on mDA neuronal cultures from fPD lines compared to its effect on the healthy line (C).', 'org/1999/xlink"" xlink:href=""cells-10-02402-g004"" position=""float""/>fPD-derived mDA neuronal cultures are more vulnerable to mitochondrial damage.']","Figure 5 fPD-derived mDA neuronal cultures are more vulnerable to mitochondrial damage. ( ) In order to assess abnormalities in mitochondrial morphology and its association with -syn accumulation, we immunostained mDA neuronal cultures derived from healthy and fPD lines with an antibody against TOM20 and -syn. mDA neuronal culture from a healthy subject presents a tubular mitochondrial network (arrow). In contrast, TOM20 staining in mDA neuronal cultures from fPD patients with A53T mutation and triplication appeared clumped (arrowhead), particularly in the cells with -syn accumulations. ( ) Abnormal mitochondrial morphology was quantified using image analysis. ( ) mDA neuronal cultures from healthy and fPD lines were treated with different concentrations of CCCP, and cell viability was assessed using the MTT assay. All data in ( ) are presented as mean SEM of three images acquired from three different coverslips. Data in figure ( ) were analyzed by one-way ANOVA followed by Tukeys multiple comparison post hoc test. * < 0.05. All data in ( ) are presented as mean SEM of four wells. Data in figure ( )were analyzed by two-way ANOVA followed by Bonferroni post hoc test. ## (Healthy line vs. triplication line at 0 M CCCP) < 0.01; ### (Healthy line vs. triplication line at 10 M CCCP) < 0.001; $$ (Healthy line vs. triplication line at 100 M CCCP) < 0.01; *** (Healthy line vs. A53T mutation line) p < 0.001; $ (A53T mutation line vs. triplication line at 10 M CCCP) < 0.05.The scale bar is 10 m.",yes
PMC3843339,Figure_1,oa_package/a6/38/PMC3843339.tar.gz,"['[14](A) Normal skin.', '[43]1(A) Cutaneous metastases seen as a nodule on the chest in a patient with carcinoma lung.', '[44]2(A) Primary cutaneous lymphoma appearing as a reddish smooth nodule.', '[45]3(A) Psoriasis seen as sharply demarcated chronic erythematous plaques covered by silvery-white scales.', '[46]4(A) Morphea presenting as multiple, superficial, ill-defined erythematous to violaceous lesions.', '[847]5(A) Contact dermatitis over the neck.', '[1148]6(A) Cutaneous lymphedema giving a Peau d orange appearance of skin over lower limb.', '[51]7(A) Infected foreign body (thorn) in soft tissues seen as echogenic focus with small surrounding collection (pus).']","Figure 1 (A) Normal skin. (B) HRUS of normal skin at 18 MHz shows the three distinctive layers of epidermal entry echo, dermis, and subcutaneous tissue",yes
PMC6658613,Figure_3,oa_package/e3/fd/PMC6658613.tar.gz,"['Our results indicated that miR-200a-3p had a potential target site in the 3 -UTR of the BACE1 mRNA (\nA\n).', 'Subsequently, the systematic diagrams of miR-200a-3p and BACE1 mRNA 3 -UTR were established (\nB\n).', 'The BACE1 mRNA is a direct target of miR-200a-3p.', 'The luminescence activity was significantly decreased in the cells that were cotransfected with miR-200a-3p mimics plus BACE1 mRNA 3 -UTR WT (\nC\n, P 0.', 'Because of the result that miR-200a-3p mimics or miR-200a-3p inhibitor did not influence the expression of BACE1mRNA at the transcriptional level significantly (\nD\n), we concluded that the interaction manner of miR-200a-3p with BACE1 relied on the regulation of the protein translation process, rather than influencing the stability of BACE1 mRNA.']","Figure 3 The mRNA is a direct target of miR-200a-3p. Bioinformatic analysis predicting the binding site of miR-200a-3p and mRNA. Design of the recombinant Luc- -MUT (mutant) and Luc- -WT (wild-type) construction. Changes in the relative luciferase activity in each group after plasmids transfection. miRNA-200a-3p mimics caused significant inhibition of reporter luciferase activity in the construct with a wild-type (WT) 3-UTR in contrast to the mutant (Mut) 3-UTR in HEK293 cells ( = 4). Quantification analysis of the levels of mRNA after transfection with miRNA-200a-3p mimics (200aM), miRNA-200a-3p inhibitors (200aI), and corresponding negative controls (NCM/NCI) ( = 3). , The expression of the BACE1 protein is signicantly decreased and increased after miR-200a-3p mimics (200aM) and inhibitor (200aI) transfection, respectively, relative to the corresponding negative controls (NCM/NCI), as demonstrated here by representative images and quantitative analysis by Western blotting ( = 3). The data are presented as the mean SEM. * < 0.05, ** < 0.01, *** < 0.001 versus relevant control.",yes
PMC11558498,Figure_17,oa_package/5d/2d/PMC11558498.tar.gz,[],"Figure 17 Finger US in longitudinal plane. (A) Rupture of the volar plate with avulsion of bone fragment at its distal insertion (white arrows). (B) Contralateral healthy side. US, ultrasound.",yes
PMC5558125,Figure_3,oa_package/9b/a6/PMC5558125.tar.gz,['Gross pathologic findings in gastrectomy specimens in two patients with giant gastric lipomas.'],"Figure 3 Gross pathologic findings in gastrectomy specimens in two patients with giant gastric lipomas. A: Patient 1. Patient 1 presented with acute melena and hemoglobin decline and had an ulcer detected at esophagogastroduodenoscopy (EGD) within a huge, lipomatous gastric mass. Gross pathologic view of the gastrectomy specimen after it is opened to expose the luminal surface shows a well-circumscribed, lobulated, 14.5 cm 9.0 cm 7.5 cm lipomatous mass extending from the gastric body (left) to antrum (right). A small ulcer (round depression, arrow) is present on the mucosa overlying the lipomatous mass. Normal gastric rugae are present above the mass on the upper left, but have been effaced on the upper right, likely because of chronic compression/pressure from the giant lipomatous mass located below (on the contralateral gastric wall before opening the stomach). Vertical incisions show a homogeneous yellow-tan cut surface, indicative of a lipomatous tumor; B: Patient 2. Patient 2 presented with melena for 3 d, orthostatic dizziness, and a hemoglobin decline to 7.1 g/dL requiring transfusion of 2 units of packed erythrocytes and had at EGD a large, yellowish, smooth, well-circumscribed antral mass. Gross pathologic view of distal gastrectomy specimen after it is opened to expose the luminal surface shows normal gastric antral tissue at left and a lobulated, well-circumscribed, yellow-tan, 9.0 cm 6.0 cm 3.5 cm lipomatous tumor at right, with a deep, clean-based, ulcer (arrow) on the mucosa overlying the mass.",yes
PMC11242036,Figure_5,oa_package/92/de/PMC11242036.tar.gz,"['A low-grade mucoepodermoid carcinoma, with a predominance of mucous cells forming cystic structures, plus foci of mucinous-rich epithelium arranged in fused papillary structures, HE 200 .']","Figure 5 A low-grade mucoepodermoid carcinoma, with a predominance of mucous cells forming cystic structures, plus foci of mucinous-rich epithelium arranged in fused papillary structures, HE 200.",yes
PMC4423901,Figure_6,oa_package/89/0a/PMC4423901.tar.gz,"['The mRNA levels were not different between IL-36R deficient and WT mice, with the exception of CXCL1, CXCL2, and IL-6 that were lower in IL-36R KO than in WT mice (A).', 'bovis BCG infection were significantly lower in IL-36R KO than in WT mice at this time-point (B).', 'g006CXCL1 expression in response to M.']",10.1371/journal.pone.0126058.g006,yes
PMC11254429,Figure_10,oa_package/72/88/PMC11254429.tar.gz,[],"Fig. 3 Esquema do procedimento cirrgico: sutura dos dois feixes do ligamento patelar (azul-escuro e azul-claro), com pelo menos 5mm de sobreposio. De notar o reforo ligamentar com feixe do tendo quadricipital (cinzento-escuro). (1Tendo Quadricipital; 2Patela; 3Ligamento Patelar)",yes
PMC7400641,Figure_7,oa_package/a3/99/PMC7400641.tar.gz,"['IHC NP staining of LN was conducted for rP18-infected guinea pig only, in which viral antigens were detected in the follicular dendritic cells and endothelial cells in sinus ().', 'IHC staining for viral nucleoprotein (NP) antigens of a representative virulent rP18-infected guinea pig (ID#867488).']","Figure 7 IHC staining for viral nucleoprotein (NP) antigens of a representative virulent rP18-infected guinea pig (ID#867488). GI, gastrointestinal tract. LN, lymph node.",yes
PMC6250019,Figure_2,oa_package/22/7b/PMC6250019.tar.gz,"['Progressive disease on imaging was correctly described on CEM and MRI (), in two women, one of them with false negative post-NAC FFDM ().', 'Our results showed that when MRI was used as the reference, CEM showed good correlation and concordance (s 2, 3, and 5).', '001""/>42-year-old woman.']","Figure 2 42-year-old woman. Invasive ductal carcinoma after neoadjuvant chemotherapy with residual disease. Pre-NAC (top row) and post-NAC (bottom row) FFDM (a), CEM (b), and MRI (c). A mass with distortion calcifications can be seen on FFDM ( ). Mass enhancement with irregular margins is shown on CEM (open arrow) and MRI (arrow).",yes
PMC7734991,Figure_7,oa_package/25/96/PMC7734991.tar.gz,"['Histopathology of lupus vulgaris shows acanthosis (black star), hyperkeratosis (white star) and dilated capillaries (yellow arrows) and granuloma (red circle).']","Figure 7 Histopathology of lupus vulgaris shows acanthosis (black star), hyperkeratosis (white star) and dilated capillaries (yellow arrows) and granuloma (red circle). Inset: Langhans giant cell. [H and E, 10]",yes
PMC11629698,Figure_1,oa_package/04/e3/PMC11629698.tar.gz,"['46 In zQ175 KI mice, exon 1 and part of intron 1 sequences of murine huntingtin (Htt) have been replaced with the human HTT exon 1 sequence carrying expanded ( 180) CAG repeats40 (A).', '\nDetection of the Htt1a transcript in the cortex of zQ175 knock-in mice.', '27 3 RACE-PCR (rapid amplification of complementary DNA ends) together with intron 1 Htt primers showed the exclusive presence of polyadenylated short Htt1a mRNA in the cortex of heterozygous zQ175 KI, but not WT littermate control mice (B).', 'End point PCR with primers for the exon 1 intron 1 boundary confirmed the presence of Htt1a in the cortex of heterozygous zQ175 KI and its absence in WT littermates (C).', 'Full-length (FL-Htt) transcripts, detected with primers that spanned exon 1 exon 2 junction, were identified in both heterozygous zQ175 KI and WT mice (C).', 'Levels of FL-Htt were determined with two different assays (primers spanning the exon 1 2 or exon 64 65 junctions, respectively), and Htt1a was quantified selectively by amplifying sequences within intron 1 (upstream of the first cryptic polyA site) and human exon 1 intron 1 (D and E).', 'Significantly lower levels of FL-Htt mRNA were detected in heterozygous zQ175 KI mice in comparison to WT mice when normalized to three housekeeping genes [D; exon 1 2 unpaired t-test, two-tailed, t(16) = 7.', '45 Consistent with end point PCR, we quantified high levels of the Htt1a transcript in the cortex of heterozygous zQ175 KI mice, but not in WT mice [E; intron 1, unpaired t-test, two-tailed, t(16) = 12.']","Figure 1 ( ) Schematic representation of wild-type (WT) huntingtin ( ) allele and chimeric mutant allele in heterozygous zQ175 knock-in (KI) mice. ( ) 3RACE RT-PCR together with intron 1 primers showed the presence of polyadenylated mRNA in zQ175 KI brain cortex, but not in WT mice. ( ) Detection of polyadenylated mRNA in the cortex of zQ175 KI mice by oligo-dT RT-PCR assay. Spliced exon 1exon 2 transcripts ( ) were detected in frontal cortex of both WT and zQ175 KI mice, whereas exon 1intron 1 product ( ) was detected only in zQ175 KI mice, but not in WT. ( ) Relative expression of in the frontal cortex of zQ175 KI mice and WT detected by TaqMan qPCR with primer-probe sets exon12 (unpaired -test, **** < 0.001) and exon6465 (unpaired -test, *** = 0.003). ( ) Relative expression of mRNA in cortical tissue of zQ175 KI and WT mice detected by TaqMan qPCR with primerprobe sets intron 1 (unpaired -test, **** < 0.0001) and human exon 1intron 1 (unpaired -test, **** < 0.0001). In and , bars show the mean standard error of the mean (SEM).",yes
PMC10572336,Figure_6,oa_package/01/89/PMC10572336.tar.gz,"['Effects of CTS, TC, and Their Combination on Protein Expression of Antioxidant Enzymes in H2O2-Treated SH-SY5Y CellsAs shown in , protein expressions of SOD (78.', 'Effect of Carthamus tinctorius L.']","Figure 6 Effect of L. seed, , and their combination on protein expression of antioxidative enzymes in H O -treated SH-SY5Y cells. The western blot bands ( ), relative protein levels of SOD ( ), CAT ( ), and GPx ( ). The values are mean SD. According to Duncans multiple range test, means with different letters (ae) are significantly different ( < 0.05). Beta-actin was used as a loading control. CTS, L. seed; TC, ; CTS + TC, a combination of CTS and TC; SOD, superoxide dismutase; CAT, catalase; GPx, glutathione peroxidase.",yes
PMC9573978,Figure_3,oa_package/e1/be/PMC9573978.tar.gz,[],"Figure3 Overview of IL-25 in APC-mutation-mediated CRC and colitis-associated cancer (CAC) models. Figure depicts the immune-regulatory role of IL-25 characterised in preclinical models of APC-mutation-mediated CRC and CAC . In the intestinal tumour microenvironment, DCLK1 tuft-like cells are the main source of IL-25, and the latter may in turn promote tumour stemness. IL-25 activates tumour IL-25R ILC2s to produce IL-4 and IL-13, which increases arginase 1 (Arg1) expression in tumour M-MDSCs leading to enhanced M-MDSC suppressive capacity. M-MDSC-mediated T cell suppression reduces T cell proliferation and IFN production, leading to impaired anti-tumour immunity and tumour progression. In the AOM/DSS model of CAC, IL-25 reduces tumour burden through eosinophils. This may be ILC2-derived IL-5 or other yet-to-be identified factors promoting eosinophil activation and cytotoxicity against tumours in a CD8 T cell-independent manner. Conversely, IL-25 may act in an autoparacrine manner to promote tumour stemness characterised by upregulation of stem markers Lgr5, CD133 and DCLK1 downstream IL-25 signaling in a Hedgehog pathway-dependent manner.",yes
PMC3692199,Figure_2,oa_package/bb/23/PMC3692199.tar.gz,[],"Figure 2 Scatterplot diagram between age and lower lingual foramen distance to crest. This plot shows a negative linear effect between age and distance of lower lingual foramen to crest ( = 0.031, = 0.248)",yes
PMC11331891,Figure_2,oa_package/90/26/PMC11331891.tar.gz,"[' 2).', 'A 61-year-old patient presence history of unresectable pancreatic cancer was treated with stent placement receiving percutaneous RFA-Stent for MBO.', 'AC: ablation catheter tipIn Stent group, only stent placement was performed.']","Fig. 2 A 61-year-old patient presence history of unresectable pancreatic cancer was treated with stent placement receiving percutaneous RFA-Stent for MBO. Axial CT images in axial and coronal CT images ( - ) shows the dilated bile ducts. Fluorescence image shows percutaneous transhepatic cholangiography. A guide wire was then passed through the stenosis. - An 8 Fr. (2.6-mm), 1.8-m bipolar RF catheter was then advanced over the wire with the tip of the catheter placed across the malignant stricture. Percutaneous radiofrequency ablation for MBO. Stent in position. A cholangiogram showing stent patency four days after the RFA treatment. AC: ablation catheter tip",yes
PMC6717619,Figure_6,oa_package/fe/ef/PMC6717619.tar.gz,[],"Figure6 a) Bronchiectasis in immunodeficiency. This patient had hyperimmunoglobulin E syndrome (Job's syndrome) and extensive bronchiectasis in the right lower lobe complicated by consolidation and an abscess formation (arrow). b) Ancillary findings with bronchiectasis. This patient had primary ciliary dyskinesia with widespread mucus plugging and adjacent consolidation, suggesting infection in the right lower lobe and middle lobe (arrowheads). The arrow points to bronchial wall thickening. Note the situs inversus.",yes
PMC10709426,Figure_5,oa_package/dd/a9/PMC10709426.tar.gz,"['5A, B).', '5C, D).', 'Local reduction of ROS in the axon delays vincristine-induced axon degeneration.', 'To further test whether reducing ROS prevented axon degeneration, neurons were treated with two potent antioxidant agents that have been shown to inhibit ROS after neuronal stress; glutathione (GSH) [38] and mitoquinone (MitoQ) [12, 39].', '5E, F).', '5G, H).']","Fig. 5 Local reduction of ROS in the axon delays vincristine-induced axon degeneration. Representative images of 5nM vincristine-treated and 5nM vincristine+50M mdivi-1 treated i Neuron axons. Staining with DHE (white). Scale bar=20m. Quantification of DHE fluorescence in 5nM vincristine and 5nM vincristine+50M mdivi-1 treated i Neuron axons shown in ( ). Results normalized to untreated axons. Results are represented as meanSEM. =4 independent differentiations, 58 images per differentiation. Two-way ANOVA, Bonferroni correction ( <0.05 *, <0.01 **). Representative images of 5nM vincristine-treated and 5nM vincristine+50M mdivi-1-treated i Neuron axons ROS quantification. Staining with mitoSOX (white). Scale bar=20m. Quantification of mitoSOX fluorescence in 5nM vincristine and 5nM vincristine+50M mdivi-1-treated i Neuron axons shown in ( ). Results normalized to untreated axons. Results are represented as meanSEM. =4 independent differentiations, 58 images per differentiation. Two-way ANOVA, Bonferroni correction ( <0.05 *, <0.01 **). Representative images of UT, vincristine, vincristine+5mM GSH, and vincristine+5M MitoQ treated i Neuron axons for 24h. Staining with DHE (white). Scale bar=10m. Quantification of DHE fluorescence shown in ( ). Results normalized to untreated axons. Results are represented as meanSEM. =3 independent differentiations, 810 images per differentiation. Two-way ANOVA, Bonferroni correction (<0.05 *, <0.01 **, <0.001 ***). Representative images of UT, vincristine, vincristine+5mM GSH, and vincristine+5M MitoQ treated i Neuron axons for 24h. Scale bar=10m. Axon degeneration index of the experiments shown in ( ). Results are represented as meanSEM. =3 independent differentiations, 810 images per differentiation. Two-way ANOVA, Bonferroni correction. ( <0.005 ***).",yes
PMC6822184,Figure_1,oa_package/20/73/PMC6822184.tar.gz,"['The prepared slides demonstrated malignant cells present in small 3-dimensional clusters with increased nuclear to cytoplasmic ratio and vacuolated cytoplasm ( 1A) consistent with non-small cell lung cancer (NSCLC).', ' 1.', '1177_2374289519881951-fig1""/>Questions/Discussion Points, Part IIWhat Are the Major Subtypes of Lung Cancer?', 'Diagnostic Findings, Part IIIThe surgical core biopsy demonstrated an infiltrative tumor consisting of glandular elements with pleomorphic and hyperchromatic nuclei ( 1B) as compared to normal histology ( 1C).', 'Immunohistochemical (IHC) findings showed that the tumor cells were positive for TTF1, Napsin-A, and negative for p40 ( 1D F) consistent with the expected pattern for an adenocarcinoma.']","Figure1. A, Cytology (fine needle aspiration [FNA]) findings of the patients lung nodule (magnification 600). The image shows a cluster of large cells with 3-dimensional structure vaguely forming an apparent glandular shape. B, Histology (core needle biopsy) findings of the patients lung nodule. Note the malignant cells lining glandular spaces and thickened alveolar septa (magnification 400). C, Histology of normal lung showing thin alveolar spaces line by small flattened pneumocytes. Few scattered intra-alveolar macrophages are noted (magnification 200). D, Immunohistochemistry findings of TTF-1 showing nuclear positivity (200). E, Immunohistochemistry of Napsin-A in this patients tumor showing granular cytoplasmic positivity (200). F, Immunohistochemistry of P40 in this patients tumor is negative (200).",yes
PMC2889648,Figure_5,oa_package/e1/8e/PMC2889648.tar.gz,['X-ray of the skull showed the splinter to be persisting [] while X-ray and contrast enhanced computed tomography (CECT) scan of the chest were normal.'],Figure 5 X-ray of the skull showing persisting splinter in the frontal area,yes
PMC8390697,Figure_3,oa_package/f2/3a/PMC8390697.tar.gz,"[' 3A C) compared to control arteries (', ' 3D F).', 'Localization of a second Asp-Pro cleavage site in NOTCH3 to cerebral arterial media in CADASIL.', 'Leptomeninges (LM; ', ' 3A) had the highest levels of protein deposition in CADASIL, and within leptomeningeal vessels, there was also predominantly medial staining.', ' 3B).', ' 3C) were stained in 15 out of 20 CADASIL patients, with the remainder of patients showing no white matter vascular staining using standardized staining conditions described.']","Figure 3 Localization of a second Asp-Pro cleavage site in NOTCH3 to cerebral arterial media in CADASIL. Immunohistochemistry using monoclonal antibody 145H at 0.6g/ml against the neo-epitope ending in Asp121 was performed on paraffin sections from CADASIL ( ) and control ( ) brain. Images of leptomeningeal (LM), gray matter (GM), and white matter (WM) arteries are shown. Additional CADASIL and control samples that were examined are shown in Supplementary Figs. and . The scale bar that applies to all images marks 100 microns.",yes
PMC183833,Figure_6,oa_package/7b/08/PMC183833.tar.gz,['Western analysis of -catenin and Fn in contracting FPCLs.'],"Figure 6 The upper panel shows the western analysis results of contracting 'stressed' FPCLs of three patient-matched disease (D) and normal/control (C) primary cell lines (n = 3). FPCLs (3 lattices per cell line) were harvested at the indicated time points following mechanical release and homogenized for protein extraction. The resulting western blots were sequentially probed with -catenin (1:750; clone 14, Transduction Laboratories), and Fn (clone IST-4, 1:500, Sigma) and Hsp47 (1:500, StressGen) antibodies. Antibody-specific bands for -catenin and fibronectin and Hsp47 were quantified using NIH Imaging software, normalized to Hsp47 levels (ratio), and plotted (bar graphs, lower panel) as the mean ratio intensity SEM per time point.",yes
PMC8378283,Figure_2,oa_package/d4/8e/PMC8378283.tar.gz,['Abdominal MRI.'],Figure 2 Abdominal MRI. Gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid-enhanced MRI showed the 28-mm-sized hepatic tumor (arrow) on T2-weighted imaging. MRI: magnetic resonance imaging,yes
PMC4056404,Figure_1,oa_package/9a/7a/PMC4056404.tar.gz,"['The root end had thin dentinal walls with apical flaring and periapical rarefaction of 2-3 mm [].', 'Preoperative radiographTreatment protocolThe tooth was anesthetized by 2% lidocaine with 1:100000 adrenaline followed by the removal of the crown.']",Figure 1 Preoperative radiograph,yes
PMC6901833,Figure_1,oa_package/ec/39/PMC6901833.tar.gz,"['Once activated, these proteins form dimers, translocate into the nucleus and either positively or negatively modulate the expression of thousands of different genes (12, 14) ().', 'Schematic presentation of the JAK/STAT pathway and the role of JAK inhibitory drugs.', 'In principle, the binding of cytokines to their receptors typically initiates an inflammatory signal ().', 'After binding, recruited JAKs initiate a signaling pathway from cellular membrane that ultimately should end in the nucleus: cytokine receptors of type I and II undergo oligomerization, leading to the recruitment of JAKs, which (auto-)phosphorylate tyrosine residues including such within the receptor chains ().']","Figure 1 Schematic presentation of the JAK/STAT pathway and the role of JAK inhibitory drugs. Binding of cytokines to receptors, which rely on the JAK/STAT pathway for signal transduction, leads to phosphorylation of JAK and STAT proteins. The latter dimerize, translocate into the nucleus and regulate the expression of inflammatory factors. JAK inhibitors (JAKi) prevent JAK phsophorylation and STAT activation. Figure was created with the help of .",yes
PMC4270725,Figure_1,oa_package/8f/69/PMC4270725.tar.gz,"[' shows light microscopy of acid-fast staining of M.', ' Greater than 95% of the amoebae were observed to be internally occupied (; Panels A C) with at least one acid-fast bacillus residing in the amoebic trophozoites.', 'leprae in cocultures of amoebae that were stained by the DNA-specific dye, DAPI, showed that the bacilli were taken up into areas that were mostly exclusive to nuclear staining indicative of primarily cytoplasmic staining ( D F).', 'g001Amoebae were cultured for 16 hr at 32 C at an M.', '1 shows the results of the Taqman analysis.', 'g0111Comparative enumeration and quantification of M.']",10.1371/journal.pntd.0003405.g001,yes
PMC11276268,Figure_12,oa_package/29/4a/PMC11276268.tar.gz,[],Figure 12 A sample image pair for in vivo knee joint testing.,yes
PMC9753838,Figure_1,oa_package/13/6b/PMC9753838.tar.gz,[],,yes
PMC4365885,Figure_3,oa_package/60/c1/PMC4365885.tar.gz,[],"Fig 3 BM HSCM reduce A burden by phagocytosis. BM HSCM were incubated for 16 hrs with a fluorochrome-tagged A42 and analysed with flow cytometry. The subpopulation of HSCM, SSC , had high A uptake as illustrated by percentage of cells with A uptake and the mean fluorescence intensity (MFI) in individual cells (A,B). The A co-localized with CD68 in lysosomes (D). Also, SSC HSCM cleared A from the APdE9 mouse brain sections substantially (C,E, = 8). To determine the reduction of total A burden, the brain sections were analysed at different time points and quantified for the remaining A42 with ELISA. BM HSCM, BMM and microglia reduced A burden (F, = 714/cell type). However, the BM HSCM reduced A faster than the other cells (F). The phagocytosis of A was not dependent on MCSF (G, = 58). Scale bar = 25 m (D) and 200 m (E).",yes
PMC11693861,Figure_4,oa_package/37/61/PMC11693861.tar.gz,"['The results showed that, compared to those in NC mice, the protein expression levels of Occludin, ZO-1, and MUC2 were decreased in arsenic-exposed mice (\nA D;\nP 0.', 'Moreover, immunohistochemistry analysis revealed that arsenic decreased the Occludin (\nE,F), ZO-1 (\nG,H), and MUC2 (\nI,J) protein expressions in the colon tissues of mice.', '\n\nEffects of arsenic on intestinal barrier and intestinal permeability changes(A) Representative western blots showing the protein levels of Occludin, ZO-1, and MUC2 in colon tissues.', '\nConcurrently, as shown in\nK,M, widely distributed FITC in the intestinal tract as well as in the serum was detected in arsenic exposed mice.', 'In addition, arsenic administration increased the bacterial loads in the liver, MLNs, and spleen (\nL), indicating that arsenic increased intestinal epithelial permeability.']",,yes
PMC9463451,Figure_5,oa_package/ef/ce/PMC9463451.tar.gz,"[' 5a, ', ' 5b).', 'Identical RCM TiME phenotypes in melanoma correlate with immune signatures.', 'Automated quantification of TiME features is feasibleNext, we investigated automated quantification of RCM TiME features immune cells, leukocyte trafficking and vasculature, using machine learning and image processing algorithms.', ' 5a).']","Fig. 5 Identical RCM TiME phenotypes in melanoma correlate with immune signatures. Unsupervised clustering of RCM features (inflammation, vasculature, trafficking) identifies two main phenotypes in melanoma lesions ( =13) that are annotated as Inflam Vasc and Inflam Vasc as shown in representative RCM and corresponding H&E images. (red arrows- vessels, cyan arrows- inflammation, yellow arrow-trafficking). Dual IHC staining for tertiary lymphoid structures using CD3 T-cells (brown) and CD20 B-cells (pink) in melanoma specimens ( =11) demonstrate higher abundance of CD3 T-cells ( -value=0.004) and TLS ( -value =0.060) in the Inflam Vasc group. Data are presented as column scatter plots and median analyzed with two-tailed unpaired Mann-Whitney test. In this analysis, =5 and =6 biologically independent samples were analyzed from Inflam Vasc and Inflam Vasc groups, respectively. RCM images were selected after reviewing images in the entire dataset. The selected images are the most representative based on PC contribution within each group. Source data are provided as a Source Data file. RCM: reflectance confocal microscopy; IHC: immunohistochemistry.",yes
PMC3510093,Figure_7,oa_package/d9/a9/PMC3510093.tar.gz,"['To evaluate the level of control that DOX has on pUS28 expression in this system, we treated cells with increasing concentrations of DOX (1 1000 pg/ml) and evaluated pUS28 expression by immunoprecipitation/western blot (A).', 'Multiple exposures of the western blots are shown to demonstrate the effects of DOX in this system (A, upper and middle panels).', 'We then evaluated US28 stimulated PLC- signaling activity at the various DOX controlled levels of pUS28 expression (B).', 'g007US28 is a potent activator of signaling even at very low expression levels.', 'g007""/>Interestingly, the effect of CCL5/RANTES on US28 stimulated PLC- signaling is negligible regardless of the absolute level of pUS28 expression (B).']",10.1371/journal.pone.0050524.g007,yes
PMC7053681,Figure_2,oa_package/f6/1d/PMC7053681.tar.gz,['Patchy areas of GGO and interlobular septal thickening involving bilateral upper lobesBlack arrowhead indicates the ground-glass opacity (GGO).'],Figure 2 Patchy areas of GGO and interlobular septal thickening involving bilateral upper lobes Black arrowhead indicates the ground-glass opacity (GGO).Blue arrowhead shows interlobular septal thickening.,yes
PMC7533056,Figure_3,oa_package/ca/35/PMC7533056.tar.gz,"['CECT found a marked increase in the size of the lower dissecting part of a previously diagnosed left-sided huge RSH, to become filling most of the left pelvis (), causing compressing and deviating toward the right side of UB.', 'CECT coronal view.', '']","Fig. 3 CECT coronal view. Black arrow showing the RSH dissected down to the pelvis, blue arrow showing inguinal extension, yellow arrow showing left abdominal wall haematoma and the red arrow showing mild perihepatic free fluid.",yes
PMC10520382,Figure_4,oa_package/39/3a/PMC10520382.tar.gz,"['Distribution of right ear hypoacusis according to subtypes and intensity.', 'Distribution of left ear hypoacusis according to subtypes and intensity.']",Figure 4 Distribution of right ear hypoacusis according to subtypes and intensity.,yes
PMC2691523,Figure_1,oa_package/20/32/PMC2691523.tar.gz,"[' 1).', '', '25 mm slice thickness, no contrast) with mask and bite block in placeFollowing CT registration, the FastPlan software detects, registers, and verifies the fiducial markers (']","Fig.1 Patient undergoing a CT (FOV 35cm, 1.25mm slice thickness, no contrast) with mask and bite block in place",yes
PMC6075040,Figure_1,oa_package/cd/a3/PMC6075040.tar.gz,"['Cell lines with undetectable LIN28A or LIN28B were selected (HeLa and HCT116), as were lines with varying levels of either LIN28 paralog (E14tg2a [a mES cell line] and Igrov1 with LIN28A expression or HEK293T and Huh7 with LIN28B expression) (A).', 'For each cell line, we used either transcription activator-like effector nuclease (TALEN) pairs or clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 to generate frameshifting insertion deletion (indel) mutations in an early coding exon (B).', 'S2) and resultant loss of protein (C), mature let-7 levels remained comparable with parental cells (', '.']","Figure 1. DIS3L2 loss does not affect mature let-7 levels in cell lines. ( ) Western blot analysis of LIN28A/B. ( ) Schematic of the gene-editing strategy used to generate knockout cell lines. Arrowheads indicate editing sites for human and mouse cells. ( ) Western blot analysis of DIS3L2 in knockout and parental cell lines. ( , ) Quantitative RTPCR (qRTPCR) measurement of let-7 family members normalized to U6 in wild-type or knockout cell lines in which neither LIN28 paralog is expressed ( ) or lines expressing either LIN28A or LIN28B ( ). Due to the lower level of expression of let-7 family members in LIN28-expressing cell lines, these values were plotted separately. = 3 biological replicates for each cell line assayed. Error bars for this and all subsequent figures represent standard deviations.",yes
PMC7608523,Figure_2,oa_package/fc/ea/PMC7608523.tar.gz,"['Before osteotomy, the height from the less affected tibial plateau (), the native slope (The height of the proximal tibial osteotomy is set from the least affected side.']",Fig. 2 The height of the proximal tibial osteotomy is set from the least affected side.,yes
PMC11330949,Figure_1,oa_package/51/e3/PMC11330949.tar.gz,"[' 1).', '', ' 1Appearance of the right atrial mass on transthoracic echocardiography']",Fig.1 Appearance of the right atrial mass on transthoracic echocardiography,yes
PMC3657244,Figure_2,oa_package/05/5b/PMC3657244.tar.gz,"['leprae consistent with lepromatous leprosy [a].', 'Histopathology of the blister showed sub-epidermal blister, with necrotizing leukocytoclastic vasculitis of papillary dermal vessels with thrombosis [b], numerous acid-fast bacilli in macrophages, and macrophage granulomas extending up to subcutis.', '(a) Atrophic epidermis, thin Grenz zone and diffuse macrophage granulomas in the upper dermis (H and E, stain at 40 magnification).']","Figure 2 (a) Atrophic epidermis, thin Grenz zone and diffuse macrophage granulomas in the upper dermis (H and E, stain at 40 magnification). (b) Papillary dermal vessels showing evidence of leukocytoclastic vasculitis (H, and E stain at 40 magnification)",yes
PMC10337658,Figure_2,oa_package/7f/bd/PMC10337658.tar.gz,"['Digital subtraction angiography (DSA) demonstrated active haemorrhage arising from the cystic artery ().', 'A repeat DSA through the microcatheter in the right hepatic artery demonstrated the cessation of flow in the cystic artery and no further active haemorrhage ().', 'DSA images demonstrating active haemorrhage from the cystic artery and subsequent coil embolization with cessation haemorrhage.']",Figure 2 DSA images demonstrating active haemorrhage from the cystic artery and subsequent coil embolization with cessation haemorrhage.,yes
PMC8909859,Figure_4,oa_package/44/95/PMC8909859.tar.gz,['(1) A melanoma in comparison with vertical LC-OCT and OCT.'],"Figure 4 ( ) A melanoma in comparison with vertical LC-OCT and OCT. An ulcerated nodular melanoma on the upper right leg. ( ) Clinical, ( ) dermoscopical, and ( ) optical coherence tomography (OCT; 6 mm 2 mm) images of a representative melanoma of the study. ( ) In vertical line-field optical coherence tomography (LC-OCT; 1.2 mm 0.5 mm) images, bright atypical melanocytic cells (red arrows) and a disturbed dermo-epidermal junction (DEJ) are visible. No dermal nests can be seen. ( ) A melanoma in comparison with horizontal LC-OCT and RCM. The same melanoma is depicted in horizontal LC-OCT (1.2 mm 0.5 mm) with an irregular honeycomb pattern with single atypical cells (red arrows) ( ), and a pagetoid spread with atypical melanocytes (red arrows) ( ). There were no dermal nests, a more chaotic structure, and no edged papillae visible. Reflectance confocal microscopy (RCM; 500 m 500 m) shows the same features. An irregular honeycomb pattern with single atypical cells, just brighter (red arrows), ( ) and a lighter pagetoid spread with atypical melanocytes (red arrows) ( ). There were also no dermal nests, a more chaotic structure, and no edged papillae visible. ( ) A melanoma in histology. The histology shows the same ulcerated nodular melanoma as in ( ) and ( ) with a tumor thickness of 4.8 mm and with 4 magnification.",yes
PMC8362226,Figure_1,oa_package/2d/09/PMC8362226.tar.gz,"['A clinical photographer took objective photographs of the patients for later comparison with the results ().', 'EB photographic examples.']","Fig 1 EB photographic examples. , DDEB. , Junctional epidermolysis bullosa. , RDEB. , Dominant dystrophic epidermolysis bullosa; , epidermolysis bullosa; , recessive dystrophic epidermolysis bullosa.",yes
PMC11587505,Figure_4,oa_package/a4/35/PMC11587505.tar.gz,"['1\ncorresponded to A ().', 'org/1999/xlink"" xlink:href=""cn4c00493_0003"" id=""gr3"" position=""float""/>Micro-ARG in brain section from an AD donor with [3H]KAC-50.']",Figure 4 Micro-ARG in brain section from an AD donor with [ H]KAC-50.1revealed accumulation of silver grains on A plaques.,yes
PMC8018819,Figure_1,oa_package/ec/82/PMC8018819.tar.gz,"['The clinical specimens were obtained from dandruff (28%), lesions on the neck (24%), lesions on the chest (12%),\nflakes behind the ears (8%), lesions on the upper back (6%), flakes around the sides of the nose (6%), lesions on the arms\n(4%), lesions of the armpits (4%), flakes of the eyebrows (4%), groin (2%), and flakes of the beard (2%; ).', 'tif"" position=""float""> Clinical signs of infection among patients; a) lesions of the armpits, b) lesions on the neck, c)\nflakes around the sides of the nose, d) flakes of beard, and e) hyphae and globose yeast cells (spaghetti and meatballs)\n(typical microscopy for Malassezia species, Gram stain, original magnification 100)Table 1Demographic and clinical characteristics of patients with different types of Malassezia infectionNo.']","Figure 1 Clinical signs of infection among patients; a) lesions of the armpits, b) lesions on the neck, c)flakes around the sides of the nose, d) flakes of beard, and e) hyphae and globose yeast cells (spaghetti and meatballs)(typical microscopy for species, Gram stain, original magnification 100)",yes
PMC10631413,Figure_2,oa_package/a8/af/PMC10631413.tar.gz,['\nComputed tomography scan images.'],"Figure 2 A: The hypodense foci, slightly off-midline to the left, at the left front of the hyoid; B: The lesion at the rear of the lower pole of the left thyroid lobe of the patient.",yes
PMC9705148,Figure_11,oa_package/dd/47/PMC9705148.tar.gz,[],Fig. 11 Intraductal papillary mucinous neoplasm ( ) Axial and ( coronal venous phase image showing three well defined cystic lesions in the pancreatic body and tail ( ) that are seen communicating with the main pancreatic duct (MPD) ( in B). MPD is normal in caliber.,yes
PMC6676523,Figure_2,oa_package/90/1a/PMC6676523.tar.gz,"[' 2).', 'Neuroendocrine and neurometabolic circuitry regulation of metabolic functions.', 'CNS: hypothalamic control of metabolic activitiesThe brain constantly monitors the metabolic states of the body.', ' 2).', ' 2).', ' 2).', ' 2) innervate -cells in islets and release acetylcholine (ACh) which potentiates -cell excitability [68, 102].', ' 2) release norepinephrine [69].', ' 2 inset).', ' 2), which regulates appetite and energy expenditure, can ameliorate symptoms of diabetes in rodent models [74].', ' 2) have been extensively mapped [11, 17, 69, 70].']","Fig. 2 Neuroendocrine and neurometabolic circuitry regulation of metabolic functions. Both afferent and efferent pathways regulate energy balance through hormones and direct neural circuits. Ghrelin, insulin and leptin are the primary hormones that mediate the sensation of satiety and hunger by activating various populations of neurons in different regions of the brain. Autonomic innervations of metabolic organs are also depicted. SNS efferent fibers control hepatic and adipocyte metabolic pathways. Vagal afferents and efferent continuously monitor and regulate systemic metabolism. Cellular metabolism, including the production and release of cytokines from the spleen, responds to the sympathetic and parasympathetic convergences in the celiac ganglion. Inset, the NPY/AgRP and POMC neurons in the ARC of the hypothalamus inversely respond to these hormones and modulate the activation of the PVN neurons that in turn regulate feeding behavior and metabolic functions. Deep brain stimulation of POMC neurons ameliorates symptoms of diabetes in rat models, and therefore may provide a therapeutic avenue for neuromodulatory treatment of metabolic diseases. Image courtesy of Anthony S. Baker and Courtney Fleming, The Ohio State University 2019; produced with permission.",yes
PMC11629275,Figure_1,oa_package/e1/0b/PMC11629275.tar.gz,"['The right eye was unable to close effectively, and the conjunctiva was red and enlarged (A, B).', 'Preoperative and postoperative appearance comparison.', 'After admission, the patient underwent a Dermatoplasty.', ""Through the surgery, the patient's facial skin basically returned (C), and the eyelids were able to close normally (""]",Fig. 1 Preoperative and postoperative appearance comparison. (A) Preoperative ocular condition; (B) preoperative tumor appearance; (C) postoperative ocular appearance; (D) postoperative eyelid closure.,yes
PMC8328747,Figure_1,oa_package/ee/70/PMC8328747.tar.gz,"['317\nCubital tunnel syndrome (right elbow) (\nA, B\n) proximal to ulnar nerve compression, there is swelling with loss of normal fascicular pattern (\nA\n).']","Fig. 1 Cubital tunnel syndrome (right elbow) ( ) proximal to ulnar nerve compression, there is swelling with loss of normal fascicular pattern ( ). Hyperintensity ( ) is seen (arrows) on axial proton-density fat saturated images. ( ) Reformatted three-dimensional short-time inversion recovery Sampling Perfection with Application optimized Contrasts using different flip angle Evolutions image clearly shows site of nerve compression by thickened retinaculum (blue arrow C, D, and F) with proximal increase in signal intensity of nerve. ( ) Ultrasound images show compressed ulnar nerve with sudden increase in caliber of nerve proximal to it (yellow arrow).",yes
PMC10891817,Figure_9,oa_package/a4/d7/PMC10891817.tar.gz,"['To also assess the accuracy of the registration process from a qualitative point of view, the overlap between the two patients CT and US is reported in .', 'The CT/US registration results for patients CB1 (a) and CB2 (b) in axial, coronal, and sagittal view.']","Figure 9 The CT/US registration results for patients CB1 ( ) and CB2 ( ) in axial, coronal, and sagittal view. The upper row shows the overlapping of CT and MV segmentation from the US for each plane. The bottom one shows the respective US planes.",yes
PMC5840488,Figure_3,oa_package/99/99/PMC5840488.tar.gz,[],"Supplemental FigureS1 Absence of concurrent RNA hybridization (ISH) ( probe targeting the 23S rRNA transcript; Advanced Cell Diagnostics catalog number 468211) and immunohistochemical colocalization within rhesus macaques with late disseminated Lyme disease. Merged image of RNA ISH (blue) and polyclonal immunofluorescent assay (IFA) (green). Green fluorescence illustrates a morphologically intact spirochete within the cerebral neuropil (IH11 doxycycline treated) with absence of concurrent labeling by the RNA ISH probe. Sample/RNA quality check on a serial section of rhesus macaque cerebrum described in . The neuropil displays intense blue punctate labeling, indicating expression of rhesus macaque housekeeping transcripts labeled with far-red cyanine 5 tyramide amplification [rhesus macaque probe targeting the peptidyl-prolyl - isomerase (PPIB) transcript; catalog number 457711]. A merged image of RNA ISH (blue) and polyclonal IFA (green). Green fluorescence illustrates two morphologically intact spirochetes within brainstem neuropil (IH22 doxycycline treated), with absence of concurrent labeling by the rRNA ISH probe. Sample/RNA quality check on a serial section of rhesus macaque brainstem as described in . The neuropil displays intense blue punctate labeling, indicating expression of rhesus macaque housekeeping transcripts labeled with far-red cyanine 5 tyramide amplification (rhesus macaques probe targeting the PPIB transcript; catalog number 457711). Original magnification, 63 (additional 2 amplification applied with Leica LAS X software when appropriate).",yes
PMC4966205,Figure_1,oa_package/24/b7/PMC4966205.tar.gz,"['Endoscopy showed an ulcero-infiltrative growth in antrum extending onto the anterior wall of first part of duodenum [].', 'Endoscopy showing an ulcero-infiltrative growth in antrum extending onto the anterior wall of first part of duodenumEndoscopy showing normal esophageal mucosaGross examination of the distal gastrectomy specimen revealed an ulceroproliferative growth measuring 8 cm 5 cm present in the antrum [].']",Figure 1 Endoscopy showing an ulcero-infiltrative growth in antrum extending onto the anterior wall of first part of duodenum,yes
PMC5582505,Figure_2,oa_package/a6/07/PMC5582505.tar.gz,['Posttreatment MRI (September 2015).'],Fig. 2 Posttreatment MRI (September 2015). Coronal and sagittal section.,yes
PMC10668929,Figure_13,oa_package/51/da/PMC10668929.tar.gz,[],Fig. 13. Small amount of trochanteric bursal fluid in a 84-year-old female with THA and concern for a pseudotumor. Long-axis US image of the greater trochanter shows trace fluid in the overlying trochanteric bursa (arrowheads). There is a small enthesophyte (arrow) adjacent to the greater trochanter,yes
PMC6000889,Figure_1,oa_package/bc/d1/PMC6000889.tar.gz,"['The soft distal tip prevents dissections unlike deep-seating of regular guiding catheters ().', 'Schematic presentation of the GuideLiner catheter.']","Figure 1 Schematic presentation of the GuideLiner catheter. . From Use of a second buddy wire during percutaneous coronary interventions: a simple solution for some challenging situations, by Burzotta et al, 2005, , 17, p. Copyright 20xx, Reprinted with permission. (A) and (B) show that wire crossing and Guideliner catheter introducing was achieved in the mid RCA. (C) A ballon could be put forward and inflated.",yes
PMC2615016,Figure_6,oa_package/5f/ff/PMC2615016.tar.gz,['Adherence and invasion of A.'],"Figure 6 . (A). Adherence of to epithelial cells. NCI-H292 cells were infected with strains at an MOI of 100 for 1 h. Actin was stained with Alexa Fluor 488 phalloidin (green). Bacteria were stained with polyclonal anti-rabbit AbOmpA antibody, followed by a secondary antibody Alexa Fluor 568 (red). (B). NCI-H292 and HEp-2 cells were infected with at an MOI of 100 for 5 h. Invasion efficiency was expressed as the number of bacteria per well using the gentamicin protection assay. Results represent the mean and standard deviation of three separate experiments on separately days. * < 0.001.",yes
PMC7541836,Figure_4,oa_package/f7/7a/PMC7541836.tar.gz,"['As shown in B the heart of infected IL-10 KO mice developed a significant interstitial inflammatory response, that fenofibrate was unable to control.', 'On the other hand, WT mice developed mild heart infiltrates upon infection with the K98 clone, regardless of fenofibrate treatment (A).']","FIGURE 4 Inflammatory reaction in the heart. Histological sections of the heart of uninfected (left panel), infected (center panel), and infected fenofibrate-treated (right panel), WT and IL-10 KO mice. Bar graphs: infiltrate score quantification. Differences between groups were analyzed by ANOVA (mean SEM) followed by Tukey test. 7 mice group, 3 replicate experiments. ** < 0.01.",yes
PMC6816007,Figure_3,oa_package/12/ef/PMC6816007.tar.gz,"['002""/>(a) Clinical presentation of vestibular papillomatosis.']",Figure 3 (a) Clinical presentation of vestibular papillomatosis. (b) Dermatoscopy showing regular and linear pinkish projections displaying a separated base.,yes
PMC3430619,Figure_1,oa_package/5b/1f/PMC3430619.tar.gz,"['parvum within their intrauterine and fetal tissues by immunofluorescent microscopy (, isotype controls can be viewed in 0.05; *= 0.05). (c) The histograms show the average occurrence of pixels ( axis) with a certain fluorescence lifetime ( axis) and represent the A conformers distribution in nA control RNAi and nA RNAi conditions. The fluorescence lifetime in the head neurons, as in (a), was measured on day 4 (left) and on day 7 (right). (d) Scatter dot plot of the average fluorescence lifetime (ns) of mScarlet of nA nematodes after systemic depletion by RNAi in the IL2 neurons. Data display the average fluorescence lifetime SD for day4 (left side) and day7old (right side) nA control RNAi (yellow) and nA RNAi (red) animals. Every dot represents the average fluorescence lifetime in the IL2 neurons of one single nematode. Three independent cohorts of 1320 animals were analyzed. Significance was tested by twoway ANOVA + Bonferroni post hoc test (ns= >0.05; ***= 0.001).",yes
PMC11469539,Figure_5,oa_package/73/ee/PMC11469539.tar.gz,"['org/repository/uniprot/P01130) (A and 5B).', 'The location of the mutation site on the three-dimensional structure of the LDLR is depicted in B.', '15), which showed that these two mutations had a minimal impact on LDLR protein structure (C and 5D).', 'g005Effects of LDLR Gly343Ser and Ala627Thr mutations on the protein tertiary structure.']",10.1371/journal.pone.0310547.g005,yes
PMC6518476,Figure_5,oa_package/a0/70/PMC6518476.tar.gz,"['In figure 5A, comparison between groups was performed using a two-way ANOVA followed by Sidak multiple comparison test.', 'For figure 5B, comparisons between groups were determined using unpaired t-tests when data followed normal distribution (Shapiro-Wilk test) and or Mann-Whitney in cases of non-normality.', '(A) Tail bleeding test revealed a significant decrease in bleeding time and blood volume in old (12/13 weeks) R6/2 mice compared with WT mice (bleeding time: 4 6 weeks, WT=6, R6/2=8; 8 9 weeks, WT=8, R6/2=9; 12 13 weeks, WT=15, R6/2=13; lost blood volume: 4 6 weeks, WT=6, R6/2=8; 8 9 weeks, WT=9, R6/2=7; 12 13 weeks, WT=9, R6/2=8).', 'However, at 12 weeks of age, when R6/2 mice exhibited a full-blown HD behavioural phenotype, blood volumes and bleeding time were significantly decreased in comparison to WT mice (figure 5A, *p 0.', 'Using standard H E staining, we observed that the vascular network occupied a significantly smaller area in HD mice than WT (figure 5B, *p 0.', 'PVG helped with all anatomical description provided in B.']","Figure 5 (A) Tail bleeding test revealed a significant decrease in bleeding time and blood volume in old (12/13 weeks) R6/2 mice compared with WT mice (bleeding time: 46 weeks, WT=6, R6/2=8; 89 weeks, WT=8, R6/2=9; 1213 weeks, WT=15, R6/2=13; lost blood volume: 46 weeks, WT=6, R6/2=8; 89 weeks, WT=9, R6/2=7; 1213 weeks, WT=9, R6/2=8). (B) H&E staining and quantification of blood vessels in 12-week-old R6/2 mice showed a significant decrease of vessel density compared with 12-week-old WT mice (R6/2, n=4; WT, n=4). Statistical analysis: two-way analysis of variance followed by Sidak multiple comparison test (A) and Mann-Whitney test (*p<0.05) (B). B, bone; h, hair; H&E, hematoxylin eosin; m, muscle; v, vessel, WT, wild type.",yes
PMC6715586,Figure_1,oa_package/23/a6/PMC6715586.tar.gz,['Abdominal computed tomography scan.'],Figure 1 Abdominal computed tomography scan. A 1.0 cm 1.8 cm focus of oval inflammatory changes surrounding central fat density visualized adjacent to the tip of the appendix and inferior aspect of the cecum noted. This is likely due to epiploic appendagitis. Possibility of very early acute distal tip appendicitis cannot be entirely excluded but felt to be less likely (Short arrow: Appendix; Long arrow: Epiploic appendagitis).,yes
PMC5826462,Figure_3,oa_package/f0/3a/PMC5826462.tar.gz,[],"Fig.3 FNA of thyroid mass (A and B DQ; C and D Pap) aspirates demonstrating a cellular smear consisting of malignant cells in loose clusters and forming glands. The tumor cells feature cytologic atypia with nuclear pleomorphism, prominent nucleoli, hyperchromasia, and necrosis. FNA, fine needle aspiration; DQ, Diff-Quik; Pap, papanicolaou.",yes
PMC11139809,Figure_12,oa_package/11/80/PMC11139809.tar.gz,[],Fig. 12 Axial PMCT image through the groin of a 16-year-old boy who was found unresponsive. Focal soft tissue oedema around the left groin (solid arrow) with a small locule of gas in the left external iliac vein (dashed arrow) is indicative of prior attempts at femoral intravenous cannulation by the ambulance team at the scene,yes
PMC11059464,Figure_2,oa_package/4a/eb/PMC11059464.tar.gz,"['Under the adipose tissue was the occipitofrontalis muscle, directly connected to the periosteum ().', '.']",Figure 2. Serial light microscopy images of hematoxylin and eosin stained sections ( ) and Alcian blue and fast red stained ( ) sections of the forehead. The stretches of connective tissue parallel to the skin and surrounding the lobules of adipose tissue are evident.,yes
PMC9775678,Figure_3,oa_package/46/02/PMC9775678.tar.gz,"['However, as expected, the IENF density was significantly decreased by about 80% in response to Taxol treatment (disease-vehicle group, and ).', 'Gabapentin significantly conferred recovery from Taxol-induced IENF loss by increasing intra-epidermal nerve fiber density to about 83% of the healthy mice ( and ) (Supplementary Materials, Data Set S4 IENF and GCPR number for all end points).', 'CGRP-I/II peptidergic nerve fiber density was significantly decreased by about 73% in response to Taxol treatment (disease-vehicle group, and ).', 'Gabapentin significantly conferred recovery from Taxol-induced IENF loss by increasing CGRP-I/II to about 46% of the level in the healthy mice ( and ) (Supplementary Materials, Data Set S4 IENF and GCPR number for all end points).', 'A schematic diagram depicting the intra-epidermal nerve fiber (IENF) counting rules and immunohistochemistry photos of punch biopsies of the different experimental groups.']","Figure 3 A schematic diagram depicting the intra-epidermal nerve fiber (IENF) counting rules and immunohistochemistry photos of punch biopsies of the different experimental groups. ( ) Diagram of skin innervation with IENF estimation rules that are widely used in human studies: nerves (red lines), basement membrane (pink area), dermis (yellow), and epidermis (blue-gray shade). (A) Count as a single unit nerve (score 1) that crosses the basement membrane of the epidermis; (B) Nerves that branch after crossing the basement membrane are also counted as a single unit (score 1); (C) Nerves that split below the basement membrane are counted as two units (score 2); (D) Nerves that appear to branch within the basement membrane are counted as two units (score 2); (E) Nerve fragments that do cross the basement membrane are counted as one unit (score 1) (F) Nerve fibers that approach the basement membrane but do not cross it are not counted (score 0); (G) Nerve fragments in the epidermis that do not cross the basement membrane in the section are not counted (score 0); ( ) Healthy micewhite arrows indicate nerves labeled with anti-PGP 9.5 antibody (red, n = 9); ( ) Taxol-treated mice (disease-vehicle)white arrows indicate nerves (n = 9); ( , ) Gabapentin-treated mice (disease-vehicle)white arrows indicate nerves (n = 9); ( ) CGRP-positive nerves (green, n = 4) represent peptidergic IENFs labeled with white arrows; Insert: DAPI labeled nuclei in blue; ( ) IENFs immunostained for PGP 9.5 (red), representing all epidermal nerve fibers labeled with white arrows; ( ) Merged F and G view; ( ). Taxol-treated mice (disease-vehicle; n = 4)white arrows indicate peptidergic IENFs; ( ) Gabapentin-treated mice (disease-vehicle; n = 4)white arrows indicate peptidergic IENFs.",yes
PMC6832928,Figure_3,oa_package/37/c1/PMC6832928.tar.gz,"['A classification of invasive procedures has proposed that describes visualization, route and purpose (the VRP Classification) [36], establishing different competences for each involved specialty: i) Per-os transpapillary route (gastroenterology); ii) per-os transmural route (gastroenterology) (); iii) percutaneous retroperitoneal route (surgeon/interventional radiologist); iv) percutaneous transperitoneal route (surgeon) (); and percutaneous transmural route (surgeon/interventional radiologist).', 'Transmural route.']",Figure 3 Transmural route. ( ) Necrosectomy through the prosthesis and ( ) purulent drainage to the gastric lumen.,yes
PMC11476780,Figure_3,oa_package/4b/a5/PMC11476780.tar.gz,"['miR-195-5p Modulates Macrophage Polarization in Intracerebral HemorrhageWe investigated the macrophage polarization effects of miR-195-5p following ICH based on the level of macrophage-polarization-related proteins detected by the western blotting (A), including CD206, CD68, arginase 1 (Arg1), inducible nitric oxide synthase (iNOS), and Akt.', '05) (A,C,E), suggesting that miR-195-5p exerts anti-inflammatory effects.', '001) (A,B,D).', '05) (A,F).', 'Effects of miR-195-5p on macrophage polarization and Akt signalling pathways following ICH (n = 5 in each group).']","Figure 3 Effects of miR-195-5p on macrophage polarization and Akt signalling pathways following ICH ( = 5 in each group). ( ) Western blot analysis of CD206, CD68, Arg1, iNOS, p-Akt, total Akt, and -actin levels between experimental groups: control, ICH (contralateral and ipsilateral), ICH + NC-mimic (contralateral and ipsilateral), and ICH + miR-195-5p (contralateral and ipsilateral). -actin was used as the loading control. Quantification of CD206 ( ), CD68 ( ), Arg1 ( ), iNOS ( ), and p-Akt/total Akt ( ), protein level normalized to -actin level. Data presented as mean standard deviation and were analyzed using KruskalWallis one-way analysis of variance and Student test for specific group comparisons. * < 0.05, ** < 0.01, and *** < 0.001 compared to control group. # < 0.05 compared between the ICH and miR-195-5p-treated groups.",yes
PMC7501437,Figure_6,oa_package/ce/9d/PMC7501437.tar.gz,"['Only when T cells were plated with CD11c+ APCs did THC increase the percentage of Tregs ( 6A); however, THC acted directly on polarized Tregs to increase the surface expression of functional markers TGF- 1 (LAP) and CD25 ( 6B), but GARP, ICOS, and CD223 (LAG-3) were unchanged ( S4F).', ' 6THC Induces Tregs through DCs to Reduce Systemic but Not Colonic InflammationLymph nodes and spleens were harvested from naive mice, and CD4+ T cells and CD11c+ APCs were selected via magnetic bead purification.', ""THC reduced serum IFN and TNF only in CD40+ IgG mice, not in Treg-depleted CD40 mice; Treg depletion did not change THC's ability to reduce serum IL-17A induced by CD40 ( 6C)."", 'Loss of Tregs prevented the reduction in spleen weight seen with THC+ CD40 IgG ( 6D).', ', 2018); however, our results indicate that THC reduces IL-22-secreting ILC3s as well as total colonic production of IL-22 independently of Tregs (s 6E and 6F).']","Figure5 CB2 Activation on Dendritic Cells Reduces Activation Markers and Increases TGF-1 Production Naive mice were administered VEH or THC (10mg/kg) once, and 24h later cLP and mLN were harvested. (A) Representative flow cytometry contour plots of dendritic cell subsets in the cLP. (B) Flow cytometry contour plots displaying FoxP3+ Helios+ nTregs and FoxP3+ Helios- iTregs (gate: Live, CD45+ CD4+) (n= 4). (C) Representative flow cytometry-overlaid histograms and median fluorescence intensity (MFI) of CCR7 expression in DCs from indicated mice in the cLP or mLN (n= 5). (D and E) (D) Total TGF-1 levels from the supernatants of cLP cells deriving from mice treated with VEH or THC for 24h (n= 56) and (E) BMDCs treated with VEH or THC after 7days of culture with treatment, or after 1day of culture after CD11c+ cell selection (n= 3). Each symbol represents an individual mouse. Data are from one experiment representative of two independent experiments and presented as mean SEM. NS, not significant: p< 0.05, p< 0.01, p< 0.005 by two-way ANOVA with Tukey's multiple comparisons test. (F) scRNA-seq violin plot of indicated mRNA expression in the myeloid cell cluster from the colon of mice treated with VEH or THC for 24 h.",yes
PMC7465433,Figure_7,oa_package/e0/25/PMC7465433.tar.gz,['Thyroid images of female rats with sequential administration of KI and sodium selenite.'],"Figure 7 Thyroid images of female rats with sequential administration of KI and sodium selenite. (A) Thyroid tissue (H&E, x4), (B) measurements of the maximum diameter of thyroid follicles (H&E, x10) (20 thyroid follicles were measured in total), (C) measurements of follicular epithelium heights (H&E, x40), (D) resorption vacuoles (VG, x20) and (E) follicular epithelium details (VG, x40). KI, potassium iodine; H&E, hematoxylin and eosin; VG, van Gieson's stain.",yes
PMC2747437,Figure_4,oa_package/9b/86/PMC2747437.tar.gz,"['[2829] (A, B)Budd-Chiari syndrome due to membranous obstruction of the inferior vena cava in the suprahepatic segment.']","Figure 4 (A, B) Budd-Chiari syndrome due to membranous obstruction of the inferior vena cava in the suprahepatic segment. Cavogram (A) shows a 4-cm long occlusion (arrow) of the inferior vena cava above the right hepatic vein (arrowhead); after needle fenestration of the occluded inferior vena cava through the femoral route, a stent is deployed across the occluded segment (B). The inferior vena cava is widely patent after stenting",yes
PMC3201596,Figure_3,oa_package/58/12/PMC3201596.tar.gz,['The ultrasound found a hepatic hydatidosis (two cysts of 65 47 mm and 70 51 mm) and a renal localization (63 51 mm) ().'],Figure 3 TDM showing hepatic and renal localization of hydatid cysts,yes
PMC8628473,Figure_5,oa_package/0a/8e/PMC8628473.tar.gz,"['5A).', '5B).', '5C J).', '5C E).', '5F).', '5G), suggesting that circVAPA knockdown suppressed the proliferation and induced the apoptosis of NSCLC cells largely by upregulating miR-342-3p.', '5H I).', '5J), indicating that circVAPA silencing suppressed the migration and invasion of NSCLC cells largely by elevating miR-342-3p level.', 'CircVAPA silencing-mediated anti-tumor effects are largely overturned by the interference of miR-342-3p in NSCLC cells.', '001MiR-342-3p interacts with the 3 UTR of ZEB2 in NSCLC cellsThe putative binding sites between miR-342-3p and the 3 UTR of ZEB2 predicted by the Starbase database are shown in ']","Fig. 5 CircVAPA silencing-mediated anti-tumor effects are largely overturned by the interference of miR-342-3p in NSCLC cells. The RT-qPCR assay was performed for examining the expression of miR-342-3p in NSCLC cells and control. The knockdown efficiency of miR-342-3p was confirmed by RT-qPCR assay in A549 and H1299 cells. A549 and H1299 cells were transfected with si-NC, sh-circVAPA#1, sh-circVAPA#1 + anti-NC, or sh-circVAPA#1 + anti-miR-342-3p. MTT and colony-forming assays were performed for examining the cell proliferation of A549 and H1299 cells. The apoptotic cells were determined by flow cytometry assay. Western blot assay was conducted to measure the expression of Ki-67 and Bax. (H-I) The transwell assay was conducted to evaluate the migration and invasion of A549 and H1299 cells. The expression levels of E-cadherin, N-cadherin, and Vimentin were evaluated by western blot assay. * < 0.05, ** < 0.01, *** < 0.001",yes
PMC6262218,Figure_7,oa_package/09/d9/PMC6262218.tar.gz,"['Strikingly, knockdown of Tmem106b also rescued the LysoTracker trafficking defect (A and B), with two independent ASOs causing a significant increase in the proportion of moving LysoTracker labelled structures in mutant CHMP2B neurons (', 'This effect was specifically due to increased trafficking as the number of LysoTracker structures within dendrites was not affected (D).', 'Interestingly, Tmem106b ASO treatment of control neurons had more modest effects, with a trend towards increased trafficking of LysoTracker structures but no effect on dendrite branching (Supplementary ).', '\nKnockdown of Tmem106b with ASOs rescues endolysosomal trafficking defects.']","Figure 7 ( ) Representative images of mutant CHMP2B neurons treated with 5 M of the indicated ASOs for 7 days loaded with LysoTracker. Scale bar = 10 m. ( ) Representative kymographs from LysoTracker live cell recordings of neurons labelled with the indicated ASOs. Vertical scale bar = 20 s, horizontal scale bar = 10 m. ( ) Quantification of kymographs of LysoTracker movement in DIV 14 mutant CHMP2B cortical cultures following treatment with the indicated ASOs. 4 with five to seven neurons per . ( ) Quantification of the number of LysoTracker labelled structures in the sections of neurite chosen for kymograph analysis. 4 with five to seven neurons per . One-way ANOVA with Newman-Keuls multiple comparison test, ** < 0.01.",yes
PMC6580697,Figure_3,oa_package/06/75/PMC6580697.tar.gz,"['This was not present in either the patient s pre-operative or early postoperative CT imaging performed at day 8 (a,b).', 'Subsequent CT imaging at 6 months also demonstrated the presence of an intravesical fat-fluid level, but with a smaller volume of the supernatant fat (d).', '.']","Figure 3. (a) Axial CT images through the pelvis at varying time points (pre-operative; 8 days, 2 months, and 6 months post-operative) demonstrating absence of fat/fluid level within the bladder (b).subsequent presence of intravesical fat as indicated by red arrowheads (c). and reduced volume at 6 months post-operative (d). (The patients ureteric stent has been removed).",yes
PMC11168961,Figure_3,oa_package/b6/6a/PMC11168961.tar.gz,"['A post-contrast sagittal T1-weighted image (T1WI) at the level of the pituitary fossa reveals the classic triad of findings indicative of pituitary stalk interruption syndrome: an ectopically placed neurohypophysis (red arrow), a thin stalk measuring less than 1 mm (blue arrow), and a hypoplastic adenohypophysis (green arrow).']","Figure 3 A post-contrast sagittal T1-weighted image (T1WI) at the level of the pituitary fossa reveals the classic triad of findings indicative of pituitary stalk interruption syndrome: an ectopically placed neurohypophysis (red arrow), a thin stalk measuring less than 1 mm (blue arrow), and a hypoplastic adenohypophysis (green arrow).",yes
PMC6018295,Figure_3,oa_package/38/e9/PMC6018295.tar.gz,"['Incision and drainage under sedationPlacement of corrugated drain postdrainageIn the second phase, calcified lesion along with soft tissue lining and impacted molar was removed under general anesthesia [s 5 7].']",Figure 3 Incision and drainage under sedation,yes
PMC8382859,Figure_6,oa_package/bc/6e/PMC8382859.tar.gz,"['Intraoperative direct portography was performed through a jejunal vein branch, in which the portal blood flow was well maintained (A).', 'An enlarged collateral vein through the inferior mesenteric vein and the lumbar vein was identified (B) and ligated.', 'Intraoperative direct portography findings.']",Fig. 6 Intraoperative direct portography findings. (A) Portal blood flow is well maintained. (B) A collateral vein through the inferior mesenteric vein and the lumbar vein is identified and ligated.,yes
PMC8468775,Figure_8,oa_package/a7/bd/PMC8468775.tar.gz,"['At 2 months of age, AT8 immunoreactivity was most predominant across the frontal cortex, particularly layers II/III (A).', 'Perikaryal and axonal staining was also strong in the pyramidal layer of hippocampal CA3 sub-region (B) and layers II/III of the entorhinal cortex (C), with less in the CA1/CA2 pyramidal layers and both the polymorph and granule layers of the dentate gyrus.', 'In the striatum, AT8 immunoreactivity was primarily evident in the fibres (D).', 'At 4 months of age, AT8 immunoreactivity was markedly increased, particularly in layer V of the frontal cortex (E) and the CA1/2 pyramidal layers (F), along with the entorhinal cortex (G) and striatum (H).', 'At 6 months of age, dendritic staining was more prominent throughout all of the investigated brain regions, and tau neuronal lesions were present sparsely in the frontal cortex (I), hippocampus (J) and entorhinal cortex (K).', 'At 8 months of age, further increased staining of the neuropil and neuronal lesions was evident in the frontal cortex (M), hippocampus (N) and entorhinal cortex (O).', 'Whereas, in the striatum, perikaryal immunoreactivity was more abundant (P).', 'At 12 months of age, atrophy was apparent in the hippocampus (R) and entorhinal cortex (S).', 'Perikaryal staining was sparse with intense staining of the neuropil and numerous neuronal inclusions in the frontal cortex (Q), hippocampus (R) and entorhinal cortex (S).', 'In comparison, however, the striatum had increased immunoreactive soma with the fibres less intensely stained (T).', 'org/1999/xlink"" xlink:href=""ijms-22-09727-g007"" position=""float""/>Immunohistochemistry with the AT8 antibody showing phosphorylated tau (Ser202/Thr205) in the frontal cortex (FC; A,E,I,M,Q), hippocampus (HP; B,F,J,N,R), entorhinal cortex (EC; C,G,K,O,S) and striatum (ST; D,H,L,P,T) of PS19 mice at 2 (A D), 4 (E H), 6 (I L), 8 (M P) and 12 (Q T) months of age.']","Figure 8 Immunohistochemistry with the AT8 antibody showing phosphorylated tau (Ser202/Thr205) in the frontal cortex (FC; , , , , ), hippocampus (HP; , , , , ), entorhinal cortex (EC; , , , , ) and striatum (ST; , , , , ) of PS19 mice at 2 ( ), 4 ( ), 6 ( ), 8 ( ) and 12 ( ) months of age. Scale bars: 50 m for each panel.",yes
PMC7702191,Figure_3,oa_package/4b/d5/PMC7702191.tar.gz,"['The measurements in this study are performed on the nucleus ( 3A, the blue area indicated by the probe), the area with the most uniform elasticity distribution, which allows avoiding artificial inflation of elasticity due to the underlying substrate.', 'The dynamics of the cellular elasticity were analyzed for each RCD modality by following 50 cells (in total) over three independent experiments (s 3C 3F).', 'During intrinsic apoptosis a rapid decrease of mechanical strength can be noticed from 1,300 to 400 Pa at 15 min post induction, which remains steady after the initial drop ( 3C), also confirming the previously reported data (Pelling et al.', 'In contrast, an increase in elasticity from 1,200 to 1,400 Pa is observed during extrinsic apoptosis at 60 min post induction preceding the fast drop to 500 Pa ( 3D).', ' 3AFM Mechano-Biology Analysis of the Elasticity Dynamics of Cells Undergoing RCD Modalities(A) A single-cell high-resolution elasticity analysis indicating the uniform elasticity distribution in the nuclear area (i.', 'Finally, for necroptosis and ferroptosis, the elasticity constantly decreases from around 1,000 Pa initially to 182 and 210 Pa, respectively, over the 5h course of the experiment (s 3E and 3F).', 'These two events are separated by 116, 40, 33, and 129 min for intrinsic apoptosis, extrinsic apoptosis, necroptosis, and ferroptosis, respectively (s 3C 3F, rectangles shaded with corresponding colors and Table S1).', '57 Pa/min for necroptosis and ferroptosis, respectively) ( 3B and Table S3).', 'Furthermore, when comparing the elasticity dynamics in different modes of RCD, clear differences could be distinguished ( 3B).']","Figure2 Morphologic Analysis of the Cellular Changes in the Early Stage of Cell Death by AFM (AD) Four-panel morphological characterization: top left, 3D AFM image; top right, whole-cell 2D AFM image; bottom left, AFM close up of RCD modality-specific characteristics; bottom right, profiles indicating geometries of typical characteristics noticed for each RCD. (A) Intrinsic apoptosis is characterized by shrinkage while maintaining adhesion points (white arrows), height profiles indicate diameters of the remaining focal adhesion points, close-up (bottom-left panel) shows observed surface irregularities in the intrinsic apoptosis. (B) Extrinsic apoptosis is characterized by total cell shrinkage and extensive blebbing (white arrows), height profiles: the average diameters of blebs. (C) Necroptosis is characterized by the overall shrinkage accompanied by the appearance of sub-micron pore-like membrane disruptions (white arrows in the close-up [bottom left panel]), height profiles: the diameter and depth of the pores. (D) Ferroptosis is characterized by the shrinkage and the presence of uniform circular membrane protrusions (indicated by white arrows), height profiles: dimensions of these protrusions. (E) Bar graph comparing three roughness parameters extracted from AFM images: Ra, the average roughness; Rq, the RMS roughness; Rt, the maximum height of the profile. For all RCD modalities, a significant increase is noted (black stars) compared with control cells (orange bars), which allows to quantitatively distinguish dead cells from viable cells based on morphological data. Both ferroptotic and extrinsic apoptotic cells also exhibit a significantly higher roughness compared with that for necroptotic cells (red stars). Error bars represent the standard deviation (n= 15). The statistical analysis was determined by a Kruskal-Wallis test followed by a pairwise Wilcoxon test with Benjamini-Hochberg adjusted p values (p< 0.05, p< 0.01, p< 0.001). See also .",yes
PMC8246960,Figure_5,oa_package/89/27/PMC8246960.tar.gz,['Comparison of the speed of improvement in clinical and patient reported outcomes.'],"Figure 5 Comparison of the speed of improvement in clinical and patientreported outcomes. (a) Median percentage improvement from baseline in Psoriasis Area and Severity Index (PASI). Data are shown as the median percentage with interquartile range. Modified baseline observation carried forward was used for missing data. Dashed lines mark 50%, 75%, 90% and 100% thresholds for improvement in PASI. (b) Median time to first achievement of PASI 50/75/90/100, Dermatology Life Quality Index (DLQI) of 0 or 1 and itch numerical rating scale (NRS) score of 0. Data are shown as the median (95% confidence interval) and were determined using KaplanMeier analyses. The intentiontotreat population was used for all analyses except for itch NRS score of 0, which used the intention to treat with baseline Itch NRS >0. 0001 for each pair of medians compared in panel (b) based on adjusted logrank test stratified by treatment. GUS, guselkumab; IXE, ixekizumab.",yes
PMC11236408,Figure_1,oa_package/1b/4a/PMC11236408.tar.gz,['.'],"Figure 1. Abdominal and pelvic computed tomography with intravenous contrast: 3.1 2.9 2.4 cm in size, intraluminal, lobulated density along the greater curve of the body of the stomach (yellow arrow).",yes
PMC8638086,Figure_3,oa_package/55/5d/PMC8638086.tar.gz,"['1177_20584601211060707-fig3"" position=""float""/>T2-weighted images and T1-weighted images: Anatomic magnetic resonance\nimaging of the prostateT2-weighted images enable to depict prostate zonal anatomy ().', '1/IMPROD bpMRI Likert score 4 5) in the peripheral zone is straightforward\nsince these lesions are focal in nature with typical PCa signal characteristics\n(decreased T2-weighted signal and associated decreased signal on ADC/ADCm images and\nincreased signal b-values acquired with strong diffusion weighting, 1500 and 2000\ns/mm2), s\n3, 13 and 16.']","Figure 3. - Prostate cancer in peripheral zone:Transversal and sagittal T2-weighted images from the same 67-year-oldman as in . In this cross-section a low-signal-intensity lesion,indicated by the arrow, is present in the left peripheral zone at thelevel of the apex. The lesion was confirmed to be Gleason score 3+4prostate cancer at targeted biopsy.",yes
PMC7566197,Figure_1,oa_package/6f/be/PMC7566197.tar.gz,"['Contrast-enhanced CT revealed a well-defined tumor that was heterogeneously enhanced with extravasation of contrast at the left adrenal site, which also showed pancreatic tail and left kidney involvement (a).', 'MRI showed that the tumor was iso-intense to hyperintense on T2-weighted imaging and had heterogeneous intensity on T1-weighted imaging without fat components (b, c).', 'Transcatheter angiography revealed extravasation in some parts of the tumor, mainly from the left adrenal artery (d).', 'Gallium scintigraphy revealed no accumulation of contrast in the tumor (e).', 'Radiological findings.', '']","Fig. 1 Radiological findings. (a) Contrast-enhanced computed tomography showing a well-demarcated tumor with heterogeneously enhanced areas of density with extravasation of the contrast. (b) Magnetic resonance imaging showing iso-intensity to hyperintensity on T2-weighted imaging (b) and heterogeneity on T1-weighted imaging without fat component (c). (d) Angiography through the left renal artery showing extravasation (arrow) in the tumor, mainly from the left adrenal artery (arrow head). (e) Gallium scintigraphy showing no accumulation of contrast within the tumor.",yes
PMC8533951,Figure_1,oa_package/82/49/PMC8533951.tar.gz,"['Historically, the potential of being translated upon exogenous delivery has made mRNA the most suitable vehicle for the expression of almost any kind of protein, including antibodies [49,50], antigens [51], or cytokines [52] (A, mRNA).', 'The addition of chemical modifications at the level of 5 -cap [54], poly(A) tail [51], or at specific nucleotides [55,56] was also instrumental for RNA stabilization and uptake (A, modRNA) [57].', 'Notably, the participation of lncRNAs in specific nuclear processes (B) opens up a chance to develop early intervention approaches to treat diseases, especially those characterized by comorbidity or without a clear genetic cause.', 'Stability is the extra value of circRNAs (C), for which the lack of free 5 and 3 -ends prevents degradation by the endogenous RNases [67].', 'MiRNAs (D) are transcribed by the RNA Pol II as long primary transcripts (pri-miRNA) and processed in the nucleus by the Microprocessor complex, which contains the RNase III-like enzyme Drosha and its cofactor DGCR8 [72].', '8916928138556Chemical and structural properties of RNA that are usable for therapeutics.']",Figure 1 Chemical and structural properties of RNA that are usable for therapeutics. Different RNA types are shown as follow: ( ) mRNA/modRNA. The uridine into pseudouridine substitution in modRNAs is represented by the greek symbol (red); ( ) lncRNA; ( ) circRNA. Locket (yellow) represents the covalently closed structure which makes circRNA resistant to exonucleases (RNases); ( ) miRNA. See text for further details.,yes
PMC10521842,Figure_1,oa_package/26/e5/PMC10521842.tar.gz,"['Mitotic activity was not conspicuous [].', 'a) Gross of borderline Brenner tumor showing solid to cystic spaces and cysts filled with mucinous materials, b) microsection shows proliferative epithelial cells of urothelial type.', '[13] Borderline or low malignant potential Brenner tumors are associated with a greater degree of nuclear atypia [].']","Figure 1 a) Gross of borderline Brenner tumor showing solid to cystic spaces and cysts filled with mucinous materials, b) microsection shows proliferative epithelial cells of urothelial type. H and E 100, c) one of the cystic cavities is lined by cuboidal epithelium (serous cystadenoma of the ovary). H & E 100, and d) microsection shows nests of benign urothelial cells with nuclear grooving. H & E 400",yes
PMC9783654,Figure_3,oa_package/57/5c/PMC9783654.tar.gz,"['It was found that the HIF-1 expression levels both on protein (a,b) and mRNA (c) were significantly increased in the hippocampus of the chronic hypoxia rats compared to those of control rats.', 'To further verify the upregulation of HIF-1 and the change of LCMT1/PP2Ac/tau phosphorylation axis induced by hypoxia, we treated rat primary hippocampal neurons with 1%O2 for continuous cultivation at different times and found that hypoxia within 48 h had no significant effect on the cell viability as suggested by the CCK8 experiment results (d).', 'Moreover, the levels of HIF-1 at 36 h and 48 h were significantly increased after hypoxia treatment (e,f).', 'Next, we detected tau phosphorylation (g i) and its kinase/phosphatase (j,k) in primary neurons with hypoxia.', 'Consistent with the experimental data derived from SD rats with chronic hypoxia treatment, 1% O2 treatment for 48 h in cultured neurons resulted in tau hyperphosphorylation as well as a marked decrease in M-PP2Ac, LCMT1 (m,n), and PP2A activity (l), while there was no change in total tau, Ser9-phosphorylated GSK-3 , total GSK-3 , T-PP2Ac and PME-1 (m,o).', 'Hypoxia upregulates HIF-1 and leads to tau hyperphosphorylation in rat primary hippocampal neurons.']","Figure 3 Hypoxia upregulates HIF-1 and leads to tau hyperphosphorylation in rat primary hippocampal neurons. ( , ) HIF-1 protein level was significantly higher in the hippocampus of hypoxia group compared with that of the control group as evaluated by western blotting, = 3 rats per group. ( ) HIF-1 mRNA expression level of hypoxia group was also increased, = 3 rats per group. ( ) CCK8 assay in primary neurons subjected to hypoxia for different times (0 h, 12 h, 24 h, 36 h, 48 h). ( ) Western blots for HIF-1 in primary neurons. ( ) Quantification of the HIF-1 protein expression levels after normalization to the -actin signal. ( ) Western blots for tau phosphorylation levels at pS199, pS262, pS396, pS404 sites, and total tau (Tau5) in neurons. ( , ) Quantification of the relative protein expression levels of pS199, pS262, pS396, pS404 tau, and Tau-5 normalization to the -actin signal. ( , ) Western blots and quantitative analysis of S9-GSK3, GSK3, the catalytic subunit of PP2A (m-PP2Ac), and total PP2Ac in neurons. ( ) PP2A activity assay in different groups in neurons. ( ) Western blots and quantitative analysis of LCMT1 and PME-1 in neurons. All data represent mean SD, = 3, * < 0.05, ** < 0.01 and < 0.001 vs. control group.",yes
PMC9777900,Figure_1,oa_package/80/2d/PMC9777900.tar.gz,"['It should be noted that the lung X-ray () from July 2019 is without changes.', '02620430112Chest X-ray 2019 Case 1.']","Figure 1 Chest X-ray2019Case 1. July 2019: no signs of interstitial damage; October 2019: reticular opacities associated with ground glass opacities with heterogeneous distribution, located subpleural especially. ( ) July 2019; ( ) October 2019.",yes
PMC8095045,Figure_3,oa_package/0b/67/PMC8095045.tar.gz,"['Distally, in both kidneys, the arteries were abnormally tortuous, with areas of occlusion and aneurysm formation (Fig 3, A).', 'The left internal iliac artery also continued as a persistent sciatic artery (Fig 3, B) and was the dominant blood supply to the left lower extremity.', 'Fig 3A, Frontal digital subtraction angiogram of one left renal artery demonstrating areas of occlusion (black rectangles) and areas of aneurysm formation (black arrows).']",Fig3 Frontal digital subtraction angiogram of one left renal artery demonstrating areas of occlusion ( ) and areas of aneurysm formation ( ). Frontal digital subtraction abdominal aortogram image demonstrating aortic quadfurcation ( ) and left persistent sciatic artery ( ).,yes
PMC11540149,Figure_1,oa_package/e7/c3/PMC11540149.tar.gz,['2Computed tomography of the abdomen and renal angiogram imagesAML: Angiomyolipoma(a) Computed tomography abdomen showing the dilated bowel loops (blue arrow) with multiple air-fluid levels indicating subacute intestinal obstruction.'],"Figure 1 Computed tomography of the abdomen and renal angiogram images AML: Angiomyolipoma (a) Computed tomography abdomen showing the dilated bowel loops (blue arrow) with multiple air-fluid levels indicating subacute intestinal obstruction. (b) Computed tomography of the abdomen showing bilateral renal AML, right inferior pole renal angiomyolipoma, and left renal angiomyolipoma replacing the kidney in its entirety with hematoma in the lower pole (red arrow). (c) Pseudoaneurysm of the left renal artery (blue arrow). Super selective renal artery embolization was done",yes
PMC11456792,Figure_1,oa_package/de/00/PMC11456792.tar.gz,"['It demonstrated an FDG avid pancreatic head mass () and no other areas of abnormal FDG uptake.', 'Of note, 4 months prior, a PET-CT performed for metastatic workup before the resection of the lung adenocarcinoma was negative for metastatic disease (), and a presumptive diagnosis of primary pancreatic adenocarcinoma was made.', '(A and B) Axial and coronal maximum intensity projection (MIP) images of the PET-CT study performed for metastatic work-up of lung adenocarcinoma demonstrating an FDG avid mass in the left lung (blue arrow) with no other areas of abnormal FDG uptake.', ': ((A-D) Axial T2, precontrast T1, arterial phase postcontrast, and venous phase postcontrast images, respectively, showing a well-defined T2 mildly hyperintense, T1 hypointense mass, with progressive postcontrast enhancement (orange arrows).']","Fig. 1 (A and B) Axial and coronal maximum intensity projection (MIP) images of the PET-CT study performed for metastatic work-up of lung adenocarcinoma demonstrating an FDG avid mass in the left lung (blue arrow) with no other areas of abnormal FDG uptake. (C) Axial PET-CT image of the same PET-CT study at the level of the head of the pancreas demonstrates no focal uptake. (D and E) Axial and coronal maximum intensity projection (MIP) images of the PET-CT performed after the diagnosis of the pancreatic head mass demonstrated an FDG avid mass (orange arrow) in the region of the head of the pancreas with no other areas of abnormal FDG uptake. On the coronal MIP images, expected tracer uptake in the bilateral renal collecting systems is also seen.",yes
PMC11241383,Figure_1,oa_package/72/92/PMC11241383.tar.gz,"['Emphysema of the right upper eyelid was also noted ().', 'By the third day of intravenous antibiotic administration, the swelling had increased in size and progressed to generalized periorbital swelling ().', 'He underwent left endoscopic maxillary antrostomy with removal of maxillary sinus tissue, left endoscopic ethmoidectomy, and then surgical orbital drainage (Video S2) via a superior transconjunctival approach 3 mm from the upper limbus (1).', 'Before discharge, clinical improvement was noted, with a decrease in left periorbital swelling (2).', '28111255472\nProgression of the right periorbital swelling.', '1Intraoperative photo following access in the orbit; note the externalization of a large amount of purulent discharge.', '2Evolution: next day after surgery (a), 2 weeks after surgery (b), and 2 months after surgery (c).']",Figure 1 Progression of the right periorbital swelling. The condition worsened on day 3 after admission: ( ) upon admission; ( ) after 3 days of conservative medical treatment.,yes
PMC3232515,Figure_5,oa_package/93/87/PMC3232515.tar.gz,"[' 5).', '', 'All with TDP-43 immunohistochemistry\nFTLD-FUSThis newest FTLD category consists of three relatively rare diseases: neuronal intermediate filament inclusion disease (NIFID) [16, 52], basophilic inclusion body disease (BIBD) [74] and atypical FTLD with ubiquitin-only immunoreactive changes (aFTLD-U) [54, 82, 103] (']","Fig.5 Histological features of FTLD-TDP types based on the classification of Mackenzie et al. Features of FTLD-TDP type 1 (Sampathu, type 3) including short, thin neurites and pleomorphic neuronal cytoplasmic inclusions (NCI) with neuronal intranuclear inclusions ( ). shows features of FTLD-TDP type 2 (Sampathu, type 1) including long, thick neurites in cortex ( ) as well as a Pick-body-like NCI in dentate fascia ( ). Features of FTLD-TDP type 3 (Sampathu, type 2) including NCI and granular cytoplasmic staining (pre-inclusions ) with irregular granular NCI in dentate fascia ( ). , Features of FTLD-TDP type 4 including NCI, neurites and many neuronal intranuclear inclusions ( ). All with TDP-43 immunohistochemistry",yes
PMC11335257,Figure_3,oa_package/11/ad/PMC11335257.tar.gz,"[' 3A, B).', ' 3B, C).', ' 3A).', ' 3B, D).', ' 3A), was also reduced in CA1-R6/2 boutons (', ' 3B, E).', ' 3B, F).', ' 3B E; Supplementary ', ' 3B F; Supplementary ', '', '0001, Kruskal Wallis with Dunn s multiple comparisons testAll together, these data show the existence of presynaptic and postsynaptic pathology at the excitatory synapse in the CA1 region of the R6/2 hippocampus, which can be reversed by normalizing the active ADAM10 level.']","Fig.3 ADAM10 heterozygous deletion in the forebrain rescues ultrastructural defects of the HD hippocampal synapse. Diagram showing SVs classification based on distance from the presynaptic membrane (docked: 050nm, reserve: 50300nm, resting:>300nm) with corresponding tenuous background colors added as a guide for the eye in TEM images reported in panel ( ). Representative TEM images of excitatory synapses in pyramidal neurons of the CA1 region of the hippocampus of WT, R6/2, R6/2-A10cKO and A10cKO mice at 13weeks of age. Scale bars: 100nm. PSD, post-synaptic density. Density of total SVs. Density of docked SVs. Density of reserve SVs. Density of resting SVs. In C-F, n=3 mice/genotype and n=60 excitatory synapses/genotype were analyzed. Each dot in the graphs represents the n SVs/m for each excitatory synapse analyzed. Data are presented as meanSEM. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, KruskalWallis with Dunns multiple comparisons test",yes
PMC6335931,Figure_5,oa_package/4f/0e/PMC6335931.tar.gz,"['The immunohistochemical staining features of PA are virtually identical to those of PMA ().', 'Immunohistochemical features of pilomyxoid astrocytoma.']","Figure 5 Immunohistochemical features of pilomyxoid astrocytoma. Positive staining for GFAP (original magnification, 200) (a) and Olig-2 (original magnification, 400) (b). Neurofilament stains may highlight an infiltrative pattern at the tumor periphery (original magnification, 200) (c).",yes
PMC5299714,Figure_6,oa_package/4c/44/PMC5299714.tar.gz,"['We propose a model to explain the underlying mechanism for the poor repair ().', 'Dysfunction and loss of mitochondria causes poor myofiber repair, which further increases calcium overload setting up a positive feedback loop for dystrophic myofiber necrosis ().', 'org/1999/xlink"" xlink:href=""cdd2016127f5""/>A model for the contribution of mitochondria in the damage of dystrophin-deficient myofiber.']","Figure 6 A model for the contribution of mitochondria in the damage of dystrophin-deficient myofiber. Absence of dystrophin increases susceptibility of myofiber sarcolemma to contraction-induced damage. Frequent sarcolemmal damage repeatedly increases cellular calcium load, which is taken up in part by the mitochondria. This calcium overload contributes to the decrease in mitochondrial activity, in accumulation at the site of injury and in mitochondrial level (potentially by mitophagy). These mitochondrial pathologies compromise the repair ability of the dystrophin-deficient myofibers, creating a positive feedback loop that results in greater necrosis of the dystrophin-deficient myofibers and increased muscle degeneration. Upregulation of dysferlin and dysferlin-related proteins is a compensatory response to this poor sarcolemmal repair, which activates the vesicular machinery for the repair of injured dystrophic sarcolemma. This increase in dysferlin-mediated repair machinery slows but fails to rescue the poor repair of dystrophic sarcolemma caused by the decline in mitochondrial function and their ability to accumulate at the site of sarcolemmal damage",yes
PMC11442150,Figure_3,oa_package/4b/87/PMC11442150.tar.gz,"['495) methods, DrugReSC maintained an advantage of at least 20% (A).', 'The consistent findings were further validated by the AUPR and accuracy results (B, C and Supplementary Tables 8-10).', 'Comparison of DrugReSC with bulk- and single-cell data-based drug repurposing methods.', '457 for the corresponding cancer types (D).', 'The consistent findings persisted across the results obtained using AUPR and accuracy as measurement metrics (E, F and Supplementary Tables 11-13).']","Figure 3 Comparison of DrugReSC with bulk- and single-cell data-based drug repurposing methods. A-C comparison of DrugReSC with bulk-sample-based drug repurposing methods DrInsight and iLINCS based on AUROC (A), AUPR (B), and accuracy (C) metrics. D-F comparison of DrugReSC with a single-cell data-based drug repurposing method Asgard based on AUROC (D), AUPR (E), and accuracy (F) metrics.",yes
PMC8669075,Figure_10,oa_package/a7/12/PMC8669075.tar.gz,[],Fig. 10 Pelvic angiogram of the same patient as in Fig. demonstrated no active extravasation. Subsequent subselection of the inferior epigastric artery also did not show any active extravasation on angiogram,yes
PMC11602569,Figure_9,oa_package/6a/9e/PMC11602569.tar.gz,"['The MB can be seen as a thick echo-dense band-like structure that traverses the right ventricular cavity and connects the inferior interventricular septum and the APM ( 9).', ' 9Echocardiographic and Anatomical Correlation of the Moderator BandEchocardiography images in parasternal long-axis (A), short-axis (B), and apical four-chamber (C) planes and their correspondence with the same type of sections in 3 human hearts.']","Figure8 Anatomical Correlation of Photon-Counting/Detector-Computed Tomography Images (A and C) Four-chamber section showing the interatrial septum and the muscular interventricular septum. Note the most common origin of the moderator band in relation to the interventricular septum in the middle zone, which occurs in almost 70% of hearts. C is an enlarged image of the zone of the moderator band exit point, showing how the band divides into a branch to the apex of the right ventricle (red dashed lines) before attaching to the base of the anterior papillary muscle. (B and D) Photon-counting detector/computed tomography images and endoscopic view showing the origin of the moderator band of the interventricular septum and its branching from the lower edge of the band toward the apex of the right ventricle (red dashed lines). LA=left atrium; RA=right atrium.",yes
PMC5291637,Figure_1,oa_package/74/6e/PMC5291637.tar.gz,[],FIGURE 1 (A) Hematoxylin-eosin staining showing papillary thyroid carcinoma (thick arrow) concomitantly with MALT thyroid lymphoma (thin arrow) (10magnification). (B) Papillary thyroid carcinoma with a predominant follicular pattern and diagnostic nuclear features (20magnification). (C) High magnification highlights sheets of plasmacytoid cells that were the predominant cell population in this MALT lymphoma (20magnification). MALT=mucosa associated lymphoid tissue.,yes
PMC7423587,Figure_3,oa_package/ff/6a/PMC7423587.tar.gz,"['\nshows serial section analyses of one of the Mycobacterium avium-intracellulare cases tested after AFB staining (panel A) and immunohistochemistry for the viral envelope protein (panel B).', 'Direct comparison of the AFB test and immunohistochemistry directed against the envelope protein of SARS-CoV-2 for the detection of Mycobacterial infections.', ')To do a more stringent test of the sensitivity of each test, six formalin fixed, paraffin embedded tissues from different cases of confirmed Mycobacterium tuberculosis infection, but where the AFB test was negative or equivocal were re-tested with each assay.', 'Representative images are shown in .']","Fig. 3 Direct comparison of the AFB test and immunohistochemistry directed against the envelope protein of SARS-CoV-2 for the detection of Mycobacterial infections. Panels A/B are serial sections of a case of tested by AFB (panel A) and by immunohistochemistry against the SARS-CoV-2 envelope protein (panel B, DAB). Note the similar intracellular distribution. Similarly, panels C/D are serial sections using these two different assays, but where the chromogen for the immunohistochemistry is Fast Red. Note the stronger signal intensity for the immunohistochemistry test (panel D). Panels E/F are serial sections of a case of documented tuberculosis where bacteria were not evident with the AFB test (panel E) but were noted with the immunohistochemistry test against the SARS-CoV-2 envelope protein (panel F, circle), using the Fast Red chromogen. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)",yes
PMC6212688,Figure_1,oa_package/43/72/PMC6212688.tar.gz,"['Some lesions showed hemorrhagic change ().', '1MalloryGWFangSGianniniCVan GompelJJParneyIFBrain carcinoid metastases: outcomes and prognostic factorsJ Neurosurg2013118889895233943372HlatkyRSukiDSawayaRCarcinoid metastasis to the brainCancer200410126052613154951813EisenhoferGBornsteinSRBrouwersFMMalignant pheochromocytoma: current status and initiatives for future progressEndocr Relat Cancer200411423436153694464Thou nnonEElkahlounAGGuillemotJIdentification of potential gene markers and insights into the pathophysiology of pheochromocytoma malignancyJ Clin Endocrinol Metab20079248654872178782475FerrariGTogniRPizzedazCAldoviniDCerebral metastases in pheochromocytomaPathologica1979717037105513906GentileSRaineroISaviLRivoiroCPinessiLBrain metastasis from pheochromocytoma in a patient with multiple endocrine neoplasia type 2APanminerva Med200143305306116774277MelicowMMOne hundred cases of pheochromocytoma (107 tumors) at the Columbia-Presbyterian Medical Center, 1926 1976: a clinicopathological analysisCancer197740198720049226548MornexRBadetCPeyrinLMalignant pheochromocytoma: a series of 14 cases observed between 1966 and 1990J Endocrinol Invest19921564364914791479Gimenez-RoqueploAPFavierJRustinPMutations in the SDHB gene are associated with extra-adrenal and/or malignant phaeochromocytomasCancer Res200363561556211450040310MannelliMLendersJWPacakKParentiGEisenhoferGSubclinical phaeochromocytomaBest Pract Res Clin Endocrinol Metab20122650751522863392Postcontrast axial T1-weighted magnetic resonance finding of intracranial metastatic PCC at preoperation.']","Fig. 1 Postcontrast axial T1-weighted magnetic resonance finding of intracranial metastatic PCC at preoperation. A: Largest one measuring 5.7 cm solid and cystic mass with hemorrhagic components and perilesional edema in left parietal lobe, compressing left lateral ventricle inferiorly. B and C: Multiple enhancing masses in bilateral cerebral hemisphere and right pons.",yes
PMC9122467,Figure_1,oa_package/1a/7a/PMC9122467.tar.gz,[],"FIGURE 1 (a) Clinical: initial presentation; hypotrichosis and multifocal crusts with paronychia of P1 and P2. (b) Caseous exudate in the claw folds in addition to paronychia and erosions. Also note the golden crusts involving the paw. (c) Clinical: initial presentation; adherent golden crusts, erosions and erythema on the inner pinnae (arrow). (d) Crusts and erythema on the planum nasale, philtrum and adjacent haired skin (arrow)",yes
PMC5013624,Figure_4,oa_package/58/b1/PMC5013624.tar.gz,"[' 4).', 'Concentration levels of several biomarkers with different type of drainage (lumbar or ventricular) across time points (horizontal lines representing the median, box representing the 25th and 75th percentiles, whiskers representing the 5th and 95th percentiles).', 'Pictures not showed for non-significant comparisonsDiscussionIn our study we assessed the possible circadian oscillation of classical (A 42, t-tau, p-tau) and candidate (A 38, A 40, sAPP , sAPP , apolipoprotein E, neurogranin, YKL-40, VILIP-1) CSF AD biomarkers.']","Fig. 4 Concentration levels of several biomarkers with different type of drainage (lumbar or ventricular) across time points (horizontal lines representing the median, box representing the 25 and 75 percentiles, whiskers representing the 5 and 95 percentiles). Pictures not showed for non-significant comparisons",yes
PMC4994105,Figure_3,oa_package/dc/d3/PMC4994105.tar.gz,[],Fig. (3) An anterior-posterior radiograph showing the center-edge angle on both hips. In this case acetabular dysplasia (CE<25) is present in both hips and there are no signs of retroversion so symptomatic PAO would be indicated on both sides.,yes
PMC10382061,Figure_8,oa_package/41/d0/PMC10382061.tar.gz,"['However, NBs that are added into a microneedle patch and used in addition to the US cause better penetration and diffusion of drugs, as depicted in [95].', 'Schematic overview of US activation of nanobubbles.']","Figure 8 Schematic overview of US activation of nanobubbles. Schematic shows the penetration of the nanobubbles through the microneedles inserted transdermally and activated through US bursting. Reprinted (adapted) with permission from Ref. [ ]. Copyright 2022, American Chemical Society.",yes
PMC9664609,Figure_4,oa_package/4f/59/PMC9664609.tar.gz,"[' 4A-D).', 'Muscle Magnetic resonance imaging (T1-weighted sequences).', 'D In the lower leg, both heads of the gastrocnemius muscle show nearly complete replacement by fat tissue and the soleus muscle shows a moderate degree of fatty infiltration whereas the anterior muscle groups are relatively sparedDiscussionMyosin myopathies are caused by dominant or recessive variants in genes encoding myosin heavy or light chains [1].']","Fig. 4 Muscle Magnetic resonance imaging (T1-weighted sequences). Preserved muscles in the shoulder girdle. Severe fatty infiltration of the rectus abdominis and oblique abdominal muscles. Moderate to major degree of fatty infiltration in all muscle groups in the thigh with the exception of the biceps femoris muscle. In the lower leg, both heads of the gastrocnemius muscle show nearly complete replacement by fat tissue and the soleus muscle shows a moderate degree of fatty infiltration whereas the anterior muscle groups are relatively spared",yes
PMC5025951,Figure_3,oa_package/17/da/PMC5025951.tar.gz,"['[4]Cerebral parenchyma, architectural distortion by lymphoplasmacytic inflammatory infiltrate with histiocytes, and some atypical cells.']","Figure 3 Cerebral parenchyma, architectural distortion by lymphoplasmacytic inflammatory infiltrate with histiocytes, and some atypical cells. Atypical cells were CD20 + with a mature T lymphocyte background",yes
PMC10592688,Figure_3,oa_package/00/fd/PMC10592688.tar.gz,"['nicotianae ortholog (A).', 'nicotianae demonstrated significantly higher xyloglucanase function (B).', 'cactorum, respectively) (B).', 'benthamiana (Supplemental A).', 'However, no significant effects of the xyloglucanase variants on chitin-triggered ROS generation were observed (Supplemental B).', 'sojae_482953 is the variant we demonstrate has a C-terminal extension that increases activity (A), and this protein triggers the highest generation of ROS in N.', 'DNS assay data (, Supplemental B) are displayed as OD readings at each timepoint; 6 h data were then analysed using a Student s t-test against the vector-only control (two-tailed, two sample equal variance) (83).', '561229v1-f0002"" position=""float""/>.']","Figure 3. Exploration of function among xyloglucanase paralogs with C-terminal extensions. _482953 (full-length) and _482953 (truncated), and orthologs in and secreted into culture supernatants were incubated with 1% (w/v) xyloglucan at 30C, pH 7; an increase in absorbance (OD ) of DNS reagent added to the samples is suggestive of an increase in the reducing sugars released (i.e. from the breakdown of the substrate). Following incubation with xyloglucan for 6 hours, we find that removal of the C-terminal extension significantly impairs xyloglucanase function for _482953 (T-test; p-value = 0.04) and its ortholog in (T-test; p-value = 0.0001), but this reduction was not found to be significant for the ortholog at any timepoint. No significant reducing sugars were detected in the vector-only sample (N=3, +/ SD). _247788 (truncated) and _247788 (full-length), and orthologs in and secreted into culture supernatants were incubated with 1% (w/v) xyloglucan at 30C, pH 7; an increase in absorbance (OD ) of DNS reagent added to the samples is suggestive of an increase in the reducing sugars released (i.e. from the breakdown of the substrate). _247788 (full-length) gave weak enzymatic activity towards xyloglucan by this method, but the orthologous proteins of and demonstrated significantly higher xyloglucanase function. The truncated orthologs were found to be enzymatically active towards xyloglucan, but with significantly reduced catalysis compared to the full-length proteins after 6 hours incubation with the substrate (T-test; p-value = 0.0002 and 0.01 for and , respectively). No reducing sugars were detected in the vector-only sample (N=3, +/ SD).",yes
PMC3306795,Figure_2,oa_package/bb/8e/PMC3306795.tar.gz,"[').', 'mov"" xlink:type=""simple"" id=""d33e2719"" position=""anchor"" mimetype=""video"" mime-subtype=""quicktime""/>Supporting Information .']","Fig. 2 Effects of nocodazol and latrunculin A on cultured astrocytes treated or not with Y27632. The left panels of ( ) and ( ) show cells treated with nocodazol (A, 30 M, 1 h) and latrunculin A (B, 5 M, 15 min). The central and right panels show cells that, upon treatment with nocodazol (A) and latrunculin A (B) were further incubated with Y27632 (25 M, 60 min) together with the cytoskeleton-addressed drugs. All cells were immunolabeled for -tubulin (green) and -actin (red). Bar in (A), valid in (B), is 10 m.",yes
PMC7737490,Figure_3,oa_package/1c/2a/PMC7737490.tar.gz,"['The results overall showed that there is wide variability in the HPF area using different hardware and software and that such differences in tissue area presented to a pathologist are divergent from those seen using a light microscope [].', 'Digital high-power field area of the same focus shown on three different monitors and native resolutions.']","Figure 3 Digital high-power field area of the same focus shown on three different monitors and native resolutions. (a-c) Aperio ImageScope; d-f Philips IMS; (a and d) Hewlett Packard Z24n G2 (1920 1200); (b and e) Philips PS27QHDCR (2560 1440); (c and f) Microsoft Surface Studio (4500 3000). The same whole slide image viewer at a 40 high-power field on different monitors and native display resolutions. Monitor resolution and size affected high-power field area the most. In addition, one web-based viewer was not compatible with the high-resolution monitor at 200% scaling (f)",yes
PMC8471674,Figure_10,oa_package/6f/bf/PMC8471674.tar.gz,[],"Figure 10 Gastrocnemius/soleus muscle tissues of mdxD2 mice treated with CAR-DCN for three weeks are able to produce more force than muscle tissues of the control mdxD2 mice. ( ) The stretch component test indicated significant force improvement in the CAR-DCN muscles. ( ) The increased force was also demonstrated when assessed by the electrical component of the test. ( ) However, mdxD2 mice treated with CAR-DCN did not show improvement in percent fatigue resistance. The solid lines indicate statistical significance ( < 0.05). = 6, 11, 9, 10 animals, respectively.",yes
PMC4290772,Figure_1,oa_package/2e/15/PMC4290772.tar.gz,"['Every tooth was attached to its pre-extraction radiograph and stored to be used as a natural model for pediatric radiography training ().', 'J Contin Educ Health Prof2003WInter23148531273925922GallagherAGMcClureNMcGuiganJCrothersIBrowningJVirtual reality training in laparoscopic surgery: a preliminary assessment of minimally invasive surgical trainer virtual realityEndoscopy1999531431031037645823SmallSDWuerzRCSimonRShapiroNConnASetnikGDemonstration of high fidelity simulation team training for emergency medicineAcad Emerg Med19994643122310230983.']",Fig 1. An extracted primary molar and the radiograph,yes
PMC11399230,Figure_7,oa_package/65/92/PMC11399230.tar.gz,"[' 7.', 'Calculation of pelvic parameters.', 'Verification of the accuracy of the DRINetTo test the performance of the DRINet in 2D femoral head segmentation, another three neural networks, including U-Net22, Res-U-Net23 and Dense-U-Net24, were used for comparison in terms of number of parameters, displacement of the central points of the femoral heads, radius error of the femoral heads, networks recognition accuracy and the Dice coefficient.']","Fig. 7 Calculation of pelvic parameters. ( , ) Automated recognition of femoral head spheres (shown as red and green spheres); ( ) Automated recognition of the superior sacral endplate (red band); ( ) Connection of the feature points to draw lines and for calculation of pelvic parameters.",yes
PMC1929071,Figure_4,oa_package/6d/ac/PMC1929071.tar.gz,['Gelatinolytic activities assessed by zymography in the muscle of normal and CXMDJ dogs.'],"Figure 4 . A. Equal amounts of protein (100 g) extracted from tibialis cranialis muscle was loaded onto a gelatin-containing SDS polyacrylamide gel. Gelatinolytic activity was identified as clear bands on a background. The activities of MMP-2, pro MMP-2 and pro MMP-9 were increased in the muscles of CXMD dogs (n = 3) compared with those in the muscle of normal control dogs (n = 3). B. Semi-quantitative analysis of the activities of pro MMP-2, MMP-2 and pro MMP-9 in control (open bar, n = 3) and CXMD (closed bar, n = 3) dogs based on analysis of the band intensity associated with each activity. Bar: mean SEM; * < 0.01.",yes
PMC2700555,Figure_2,oa_package/4a/f9/PMC2700555.tar.gz,"['OVA-treated RBP-jCKO animals developed a more severe lung inflammation compared with OVA-treated wild-type littermates ().', 'In the surviving mutants, the number of bronchoalveolar lavage (BAL) leukocytes and the percentage of BAL eosinophils were significantly higher compared with those of the wild-type littermates (A and 2B).', 'In addition, IgE was detectable only in the BAL fluid of RBP-j deficient animals (C).', 'Histology of the lungs from OVA-challenged RBP-jCKO and wild-type controls clearly confirmed the existence of severe airway inflammation in RBP-jCKO mice, including significant leukocyte infiltration around the airways and blood vessels, extensive goblet cell hyperplasia in the large airways, and distinct appearance of goblet cells in medium-sized airways (D).', 'g002Five- to seven-wk-old RBP-jCKO mice develop a severe allergic lung inflammation in an OVA-induced murine model of asthma.']",10.1371/journal.pbio.1000067.g002,yes
PMC10410444,Figure_3,oa_package/39/a5/PMC10410444.tar.gz,"['1%) tested positive for CK (Table 3 and ).', 'Immunohistochemical features of the DPM.']","Figure 3 Immunohistochemical features of the DPM. Immunohistochemical staining showed that cells were positive for CD56, EMA, PR, and vimentin, negative for CK and TTF-1. CK, cytokeratin; EMA, epithelial membrane antigen, PR, progesterone receptor, TTF-1, thyroid transcription factor.",yes
PMC3287108,Figure_3,oa_package/ed/7b/PMC3287108.tar.gz,['Histopathological analyses of quadriceps muscle sections from DKO mice.'],"Figure 3 . H & E-stained quadriceps muscle sections from dysferlin-null, and DKO mice at six months of age. Scale bar: 100 m. Quantitative analysis of centrally nucleated muscle fibers (CNF) in quadriceps muscles from WT, dysferlin-null, and DKO mice ( = 6 per group) at six months of age. Each group was significantly different from all the other groups. For clarity, significance is shown only for the comparisons with the DKO mice. *** < 0.001.",yes
PMC10692797,Figure_4,oa_package/71/12/PMC10692797.tar.gz,"['The autopsy was conducted 4 hours after death (A E).', 'Table 2Macroscopic and microscopic findings in each organ.']","Fig. 4 Pathologic findings of the lungs and trachea on autopsy in a Japanese man who died from COVID-19. (A) Gross findings show congested lungs and marked emphysematous changes mainly in the upper lung lobes. Microscopic lung findings show (B) organization in the left upper lobe, (C) accumulation of neutrophils in an organized air cavity in the right lower lobe, (D) neutrophilic infiltration in the tracheal mucosa, and (E) thrombus in a pulmonary vessel. (Hematoxylin and eosin stain; B, D, and E200; C40).",yes
PMC6628284,Figure_2,oa_package/0a/46/PMC6628284.tar.gz,"['The MRI protocol used for prostate volume (PV) measures included a T2-weighted turbo spin echo sequence in the transversal and sagittal plane, performed on a 3-Tesla scanner using a phased-array 32-channel body coil, see a,b.', '(a) Benign prostatic hyperplasia in a patient demonstrated on magnetic resonance imaging with T2-weighted turbo spin echo sequence in the transversal plane.']",Figure 2 ( ) Benign prostatic hyperplasia in a patient demonstrated on magnetic resonance imaging with T2-weighted turbo spin echo sequence in the transversal plane. Notice the adenoma in the left lobe compressing the prostatic urethra; ( ) the same patient three months after treatment with a reduced T2 signal corresponding to infarction of the adenoma.,yes
PMC8688964,Figure_4,oa_package/99/e7/PMC8688964.tar.gz,[],Fig. 4 En bloc retrieved PICC line grasped by loop snare,yes
PMC2972610,Figure_11,oa_package/4e/f0/PMC2972610.tar.gz,[],"Figure 11 It is very useful to perform the 3D data on the Salter Harris criteria on the young patients with epiphyseal trauma. A fracture line ( ) through the physis extending up into the metaphysis (Type II) was corrected as type IV by 3D image (thick arrow ). In another case with fibulary malleolar fracture ( ), classification also was corrected by 3D imaging findings (thick arrow in ) (conversion of type IV to type II).",yes
PMC9438229,Figure_3,oa_package/02/93/PMC9438229.tar.gz,"[' 3a).', ' 3b), indicating that lysine residues of -Syn are critical for LRP1-mediated -Syn uptake.', 'LRP1 regulates -Syn uptake via lysine residues in the N-terminus of -Syn.', '001We next sought to determine whether LRP1 regulates -Syn uptake through the recognition of the lysine-rich N-terminus of -Syn.', ' 3a).', ' 3c).', ' 3d).']","Fig. 3 LRP1 regulates -Syn uptake via lysine residues in the N-terminus of -Syn. Schematic diagram of -Syn domains highlighting the lysine residues (K). Uptake of -Syn and lysine-capped -Syn in WT iPSNs. , Uptake of -Syn-488, N--Syn-488 and N--Syn-488 in WT and -KO iPSNs. Uptake of -Syn-488 in the presence of excessive non-labeled -Syn N-terminus (N--Syn) or -Syn lacking N-terminus (N--Syn) fragments. All experiments in ( ) were performed in technical duplicates or triplicates over three independent experiments. All data are expressed as means.d. with individual data points shown. Data in ( ) was analyzed by unpaired two-sided t-test. *** <0.001. Data in ( and ) were analyzed by One-way ANOVA with Tukeys multiple comparisons test. NS, not significant; * <0.05, ** <0.01, *** <0.001",yes
PMC5705348,Figure_2,oa_package/ac/5c/PMC5705348.tar.gz,"['The conceptual framework for these SC niches, their structure, compositions and operating process is steadily being updated ()27282930.', 'The hierarchical model states that the epidermis is built of discrete epidermal proliferative units with a central slow-cycling stem cell that yields rapidly dividing TACs, which departs from the basal layer after several divisions to generate upward columnar units of differentiating cells.']","Fig. 2 The hierarchical model states that the epidermis is built of discrete epidermal proliferative units with a central slow-cycling stem cell that yields rapidly dividing TACs, which departs from the basal layer after several divisions to generate upward columnar units of differentiating cells. The stochastic model suggests that the epidermal basal layer is composed of a single type of proliferative progenitors whose daughter cells choose randomly to differentiate or remain as progenitors. Each division of basal cells can yield three different outcomes: (1) one differentiated daughter that withdraws from cell cycle and leaves the basal layer, and one progenitor that remains in the basal layer, continue to divide; (2) two differentiated daugthers; and (3) two basal progenitors. Although the fate choices are random, the probabilities of different outcomes are similar, so that the generation of differentiated cells and the maintenance of committed progenitor pools are balanced, guaranteeing long-term homeostasis. Predictions of lineage-tracing results from each model are shown via the red stained cells; cells with prominent red colors are the ones retaining lineage-traced marks . SC: stem cell, TAC: transit amplyfying cell (rapidly dividing), PC: dividing progenitor cell.",yes
PMC4495121,Figure_5,oa_package/bf/b0/PMC4495121.tar.gz,"['Interestingly, recent OCT studies demonstrate that some neoatheromas evolve into calcifications located inside of the stent () [35].', 'Late outcomes of percutaneous coronary intervention by OCT.', 'Note that PCI of bifurcations is an independent risk of malapposition and in-stent thrombosis; hence the precise guidance by OCT can reduce the risk () [41].']","Figure 5 Late outcomes of percutaneous coronary intervention by OCT. Covered struts in malapposition. Underexpansion of the stent. Chronic vessel dissection; true lumen (TL), false lumen (FL). Heterogeneous neointima in the stent (arrow indicates stent strut). Thin fibrous cap covering neoatheroma (50 m); Plaque protrusion after balloon angioplasty in the restenotic stent",yes
PMC4126351,Figure_2,oa_package/c9/21/PMC4126351.tar.gz,['Endoscopic submucosal dissection (ESD) procedure for esophageal granular cell tumor (GCT).'],Figure 2 Submucosal injection of mixed saline solution. Circle labels the edge of the GCT. The surface of the wound (muscularis propria layer). The specimen fixed and measured.,yes
PMC2943100,Figure_1,oa_package/d1/52/PMC2943100.tar.gz,"['At the age of 10 months an extensive neuron loss ( 50%) in the hippocampus was reported, that correlated with the accumulation of intraneuronal A and Thioflavin-S positive intracellular material [18] ().', 'In press38SaidoTCIwatsuboTMannDMAShimadaHIharaYKawashimaSDominant and differential deposition of distinct -amyloid peptide species, A N3(pE), in senile plaquesNeuron1995142457466785765339SchillingSLauberTSchauppMOn the seeding and oligomerization of pGlu-amyloid peptides (in vitro)Biochemistry2006454112393123991702939540KuoY-MWebsterSEmmerlingMRDe LimaNRoherAEIrreversible dimerization/tetramerization and post-translational modifications inhibit proteolytic degradation of A peptides of Alzheimer s diseaseBiochimica et Biophysica Acta199814063291298963068141RussoCViolaniESalisSPyroglutamate-modified amyloid -peptides A N3(pE) strongly affect cultured neuron and astrocyte survivalJournal of Neurochemistry2002826148014891235429642WirthsOBreyhanHCynisHSchillingSDemuthH-UBayerTAIntraneuronal pyroglutamate-A 3-42 triggers neurodegeneration and lethal neurological deficits in a transgenic mouse modelActa Neuropathologica200911844874961954799143TomiyamaTNagataTShimadaHA new amyloid variant favoring oligomerization in Alzheimer s-type dementiaAnnals of Neurology20086333773871830029444BillingsLMOddoSGreenKNMcGaughJLLaFerlaFMIntraneuronal A causes the onset of early Alzheimer s disease-related cognitive deficits in transgenic miceNeuron20054556756881574884445Bertoni-FreddariCSensiSLGiorgettiBDecreased presence of perforated synapses in a triple-transgenic mouse model of Alzheimer s diseaseRejuvenation Research20081123093131832800846GrueningerFBohrmannBCzechCPhosphorylation of Tau at S422 is enhanced by A in TauPS2APP triple transgenic miceNeurobiology of Disease20103722943061978164547RobersonEDScearce-LevieKPalopJJReducing endogenous Tau ameliorates amyloid -induced deficits in an Alzheimer s disease mouse modelScience2007316582575075417478722Hippocampal neuron loss in the APP/PS1KI mouse model of AD.']","Figure 1 Hippocampal neuron loss in the APP/PS1KI mouse model of AD. (a) APP (brown) and A staining (green) in the hippocampal formation of a 2-month-old and (c) a 10-month-old APP/PS1KI mouse. (b,d) Higher magnification of the CA1 granular cell layer of a 2-month-old (b) and a 10-month-old APP/PS1KI reveals profound neuron loss at the later time point. Scale bars: (a,c): 200 m, (b,d): 50 m.",yes
PMC9106345,Figure_1,oa_package/58/f4/PMC9106345.tar.gz,"['Cre expression resulted in deletion of Zfp36l2 at birth, which was confirmed at the genomic, RNA, and protein level ().', 'As expected, hearts from Zfp36l2-KO mice did not display any change in the levels of ZFP36 or ZFP36L1 proteins (Supplemental , A and B; supplemental material available online with this article; Several focal areas with hemorrhage and edema were observed in all dengue cases, located preferentially in the medullar region (c).', 'Circulatory disorders were also observed in semi-thin sections revealing the presence of thrombus in glomerular capillaries (g).', 'Detection of Dengue Virus in the KidneyVirus antigens were detected by immunohistochemical analysis, revealing that all dengue cases presented similar number of positive cells (m), mainly in circulating macrophages and monocytes into blood vessels (']",10.1371/journal.pone.0083386.g007,yes
PMC4065024,Figure_3,oa_package/ba/2e/PMC4065024.tar.gz,"['In cerebellar granule cells we demonstrated co-localization of hnRNP A1, hnRNP H1/F, ALYREF and SRSF2 with 27%, 30%, 26% and 33% of RNA foci, respectively (A D).', 'In motor neurons, the cell type most vulnerable to the neurodegenerative process in ALS, we demonstrated co-localization of hnRNP H1/F, ALYREF and SRSF2 with 19%, 29% and 30% RNA foci, respectively (E G).', '1093/brain/awu120/-/DC1"">Supplementary ).', 'Combined RNA FISH and immunohistochemistry demonstrate co-localization of nuclear speckle components with RNA foci in CNS tissue.']","Figure 3 Combined RNA FISH and immunohistochemistry demonstrate co-localization of nuclear speckle components with RNA foci in CNS tissue. hnRNP A1 ( ), hnRNP H1/F ( ), SRSF2 ( ) and ALYREF ( ) are observed to co-localize with RNA foci (arrowheads) in cerebellar granule cells from + patients with ALS. hnRNP H1/F ( ), SRSF2 ( ) and ALYREF ( ) are observed to co-localize with RNA foci (arrowheads) in nuclei of motor neurons from + patients with ALS. Co-localization events are enlarged and unmerged protein and RNA foci are shown for comparison. The normal staining pattern of the two proteins in cases with ALS and control subjects is included for comparison. Scale bar = 3 m.",yes
PMC8576332,Figure_5,oa_package/ba/33/PMC8576332.tar.gz,[],"Figure4 The suppression of NF-B mediated metastasis ability by imipramine in CL1-5-F4 cells. The migration pattern and quantification of gap area after treatment with 100 and 150 M imipramine are performed by wound healing assay and displayed. The crystal violet staining results of wound healing assay after 20 hr migration are presented. The transwell migration, invasion and quantification bar chart after imipramine treatment are displayed. The protein expression of MMP9, VEGF, MCL-1, cFLIP, XIAP and their quantification results are displayed. ( < 0.01 vs. 0 M imipramine; < 0.01 . 100 M imipramine).",yes
PMC11272914,Figure_5,oa_package/76/f4/PMC11272914.tar.gz,"['Case 1: Clinical and radiological images(A) Clinical image of swelling in maxillary left canine-premolar region vestibule; (B) Surgical image; (C) X-ray image; (D) Surgical specimenOn fine needle aspiration, a straw-coloured fluid with pus was found.']",Figure 5 Case 1: Clinical and radiological images (A) Clinical image of swelling in maxillary left canine-premolar region vestibule; (B) Surgical image;(C) X-ray image; (D) Surgical specimen,yes
PMC10558894,Figure_1,oa_package/67/1d/PMC10558894.tar.gz,"['A 39-year-old male with hemophilic arthropathy.', '1A 55-year-old male with partial articular surface supraspinatus tendon avulsion (PASTA).', '2A 35-year-old male with an avulsion fracture of the supraspinatus tendon.', '3A 45-year-old female with a malunited fracture of the proximal humerus (neck).', '4A 39-year-old female with infraspinatus myositis.', '5A 27-year-old male with a sprain of supraspinatus in an operated case of arthroscopic repair of supraspinatus.', '6A 46-year-old male with supraspinatus tendinopathy due to external impingement.', '7A 51-year-old male with a complete tear of the supraspinatus tendon, a partial tear of the infraspinatus tendon, and the bicipital tendinopathy.']","Figure 1 A 39-year-old male with hemophilic arthropathy. Axial fat-suppressed proton density (A), sagittal fat-suppressed proton density (B), and coronal susceptibility-weighted imaging (C) show erosive destruction of the head of humerus {H} with loss of contour and erosions of the visualized part including the greater and lesser tuberosity and glenoid margins (arrows) with hypo intensity of visualized subscapularis {Sub}, supraspinatus {S} and infraspinatus {Inf} on fat-suppressed proton density and blooming on susceptibility-weighted imaging. Image credits: Anshul Sood",yes
PMC1622750,Figure_4,oa_package/0e/da/PMC1622750.tar.gz,['Nests of non keratinizing squamous cell carcinoma (H E 200 ).'],Figure 4 Nests of non keratinizing squamous cell carcinoma (H&E 200).,yes
PMC2386496,Figure_2,oa_package/9c/c2/PMC2386496.tar.gz,"['Scheme of the fixation of the SIS with a running suture, mimicking the fixation of a drumhead.']","Figure 2 Scheme of the fixation of the SIS with a running suture, mimicking the fixation of a drumhead.",yes
PMC8559633,Figure_1,oa_package/5d/be/PMC8559633.tar.gz,"['Another small pleural based consolidation was also seen in the superior segment of the left lower lobe (figure 1.', 'CT scan of a man with spontaneous pneumothorax due to the COVID-19a) The first CT scan showed consolidation with air-bronchogram in the right upper lobe.', 'Patchy ground-glass opacity with superimposed septal thickening (crazy paving pattern) was seen in the right upper lobe too (figure 1.', 'A subpleural band-like opacity was also seen in the anterior portion of left lower lobe and right upper lobe, and patchy ground-glass opacity was more predominant in the left side (figure 1.']",Figure 1 CT scan of a man with spontaneous pneumothorax due to the COVID-19,yes
PMC10792151,Figure_12,oa_package/b8/88/PMC10792151.tar.gz,[],"Fig. 12 Intrathecal methotrexate (MTX) administration complicated by epidural hematoma. A 66-year-old man was on an intrathecal MTX regimen for treatment of non-Hodgkin lymphoma, presented with severe back pain that radiates to the left leg after the first week of intrathecal MTX administration. Sagittal T1- ( ) and T2-weighted ( ) MRI lumbar spine images demonstrate a posterior epidural collection of iso to slightly high T1 and mixed heterogeneous T2 signal intensities, compressing the thecal sac with crowding of cauda equina (arrows). Axial T2WI ( ) shows corresponding high T2 intensity epidural collection compressing the right posterolateral aspect of the thecal sac (thick arrow). Axial T1W post-contrast image reveals no abnormal enhancement. The findings are consistent with an epidural hematoma. Besides, there is an old compression fracture of the L1 vertebral body which has been stable since the prior study (not shown). Patient underwent an urgent laminectomy and evacuation of the epidural hematoma with subsequent improvement of his symptoms",yes
PMC11443099,Figure_1,oa_package/a9/0e/PMC11443099.tar.gz,"[' 1a).', ' 1b, c, Supplementary ', ' 1d) as well as in axonal fibers from M1 to the dorsal striatum (DS) (Supplementary ', ' 1e), whilst their abilities to acquire complex motor skills were disrupted.', ' 1f).', ' 1g, h).', 'Human -synuclein resulted in motor learning deficits associated with cortical hyperexcitability.', 'Cortical neuronal activity is closely associated with motor learning functions, and hyperexcitability of the cortex has been reported in neurodegenerative diseases19,20.', ' 1i) of layer 5 pyramidal neurons (L5 PNs).', ' 1j, k).', ' 1l), plus higher values of afterhyperpolarization (AHP), Rheobase and resting membrane potential (RMP) (P 0.', ' 1m o).']","Fig. 1 Human -synuclein resulted in motor learning deficits associated with cortical hyperexcitability. Upper, the design of adeno-associated virus (AAV)- human synapsin (hSyn)-human -synuclein (SNCA)-mCherry viral vector. Lower, Schematic diagram of generating mouse synucleinopathy model by expressing SNCA gene into bilateral primary motor cortex (M1) of adult male mice under the help of an AAV vector carrying hSyn promoter. Three weeks later, behavioral test batteries were performed. Representative blotting bands of total synuclein and phosphorylated synuclein in M1 extracts. Quantification for the relative expression of total (left) and phosphorylated synuclein (right). Two-sample unpaired -test, (8)=6.967, =0.0001 for synuclein; (8)=3.907, =0.005 for p-synuclein. =5 mice in each group. Upper panels: coronal section of mouse M1 after SNCA overexpression. AAV-SNCA was mostly expressed within neurons (NeuN+). Lower panels: cortical expression of synuclein and phosphorylated synuclein were observed. Scale bar, 100 m or 20 m (enlarged panels in lower right). Total distance traveled in the open field arena was unaffected after SNCA overexpression. Two-sample unpaired -test, (14)=0.3807, =0.7091. SNCA-overexpressing mice had remarkably impaired motor performance on the accelerating rotarod. Two-way analysis of variance (ANOVA) with respect to group factor, (1126)=55.81, <0.0001. Sidaks multiple comparison test of post hoc comparison at each time point. SNCA group spent longer time to accomplish the vertical climbing task. Two-way ANOVA with respect to group factor, (142)=4.553, =0.0387. Sidaks multiple comparison test of post hoc comparison at each time point. Worsening performance on the walking beam in mutant mice. (139)=13.55, =0.0007. Sidaks multiple comparison test of post hoc comparison at each time point. =8 mice in each group in ( ). Patch-clamp recording traces of layer 5 pyramidal neurons (L5 PNs) showed potentiated miniature excitatory postsynaptic currents (mEPSCs) in SNCA group. SNCA group had higher amplitude of mEPSCs. Non-parametric MannWhitney test =6, =0.018. Increased frequency of mEPSCs was observed under synucleinopathy. Non-parametric MannWhitney test =6, =0.017. Elevated number of action potentials (AP) in SNCA group under fixed injection currents. Two-way ANOVA with respect to group factor, (1104)=4.530, =0.0357. Higher potentials of afterhyperpolarization (AHP). Two-sample unpaired -test, (13)=3.817, =0.0021. Higher rheobase values in SNCA group. Two-sample unpaired -test, (13)=3.026, =0.0097. Decreased resting membrane potential (RMP) in SNCA group. Two-sample unpaired -test, (13)=4.181, =0.0011. =8 and 7 neurons from 3 animals in mCherry and SNCA group, respectively, in ( ). ns, no significant difference, * <0.05, ** <0.01, *** <0.001, **** <0.0001. All data were presented as meanSEM.",yes
PMC6922538,Figure_1,oa_package/9c/85/PMC6922538.tar.gz,['MRI was performed at initial admission.'],"Figure 1 MRI was performed at initial admission. A 50-year-old male diagnosed as PHNET, grade G2. (A) Hybrid hypointense was observed on T1WI, and pseudocapsule could be seen. (B) Axis T2WI showed mixed high signal. (C) DWI image, (D) Enhanced anterior mask. (E) Early enhancement of arteries. (F) Late enhancement of arteries. (G) Enhanced venous phase. (H) Enhancement delay period.",yes
PMC8138496,Figure_1,oa_package/02/46/PMC8138496.tar.gz,"['The biopsy () showed small, mature, lymphocytes, a reactive secondary follicle, numerous eosinophilic granulocytes and a large number of medium sized round-oval cells, with grooved, folded, indented, or lobed nuclei, with fine chromatin, small nucleoli, delicate nuclear membrane, and abundant pale eosinophilic cytoplasm, suspicious for Langerhans cells.', 's and tables.']","Figure 1. Needle biopsy (14 G). (A) Lymphoid tissue with pale areas (hematoxylin-eosin). (B) Medium sized cells, with folded, indented nuclei, fine chromatin, small nucleoli, delicate, and abundant pale eosinophilic cytoplasm, with a lot of eosinophilic granulocytes (hematoxylin-eosin). (C) CD68R/PG-M1 (immunohistochemistry) positive in occasional macrophages. (D) Myeloperoxidase (immunohistochemistry) positive in granulocytes. (E) S100 protein (immunohistochemistry) positive in Langerhans cells. (F) CD1a (immunohistochemistry) positive in Langerhans cells.",yes
PMC2728191,Figure_1,oa_package/ff/c3/PMC2728191.tar.gz,"['A single centre experienceLiver Int20062643381662964620LempinemMHalmeLNumminenKArolaJNordimAMakisaloHSpontaneous rupture of a hepatic cystadenoma and cystadenocarcinoma: report of two casesJ Hepatobiliary Pancreat Surg200512409141625881121JovineEBiolchiniFTalaricoFLandolfoGMartuzziFIntrahepatic rupture of a caudate lobe adenoma in liver adenomatosisJ Hepatobiliary Pancreat Surg200411324291554943122WeimannARingeBKlempnauerJLameschPGratzKFProkopMBenign liver tumours: differential diagnosis and indicators for surgeryWorld J Surg19972198390936151523AmeriksJAThompsonNWFreyCFAppelmanHDWalterJFHepatic cell adenomas, spontaneous liver rupture, and oral contraceptivesArch Surg19751105487113100024BennetWFBovaJGReview of hepatic imaging and a problem oriented approach to liver massesHepatology19901276175221067925HussainSMvan den BosIDwarkasingRSKuiperJWden HollanderJHepatocellular adenoma: findings at state-of-the-art magnetic resonance imaging, ultrasound, computed tomography and pathologic analysisEur Radiol2006161873861670821826ReddyKRKligermanSLeviJLivingstoneAMolinaEFranceschiDBenign and solid tumors of the liver: relationship to Sex, age size of tumors and outcomeAm Surg20016717381124354527GordonSCReddyKRLivingstoneASJeffersLJSchiffERResolution of a contraceptive-steroid-induced hepatic adenoma with subsequent evolution into hepatocellular carcinomaAnn Int Med19861055479301920128EdmonsonHAHendersonBBentonBLiver cell adenomas associated with use of oral contraceptivesN Engl J Med1976294470217399629WeimannARingeBKlempnauerJLameshPGratzKFProkofMBenign liver tumors:differential diagnosis and indications for surgeryWorld J Surg19972198390936151530TerkivitanTde WiltJHde ManRAvan RijnRRZordevanPETilanusHWManagement of hepatocellular adenoma during pregnancyLiver20002018671084749031TerkivitanTde WiltJHde ManRAvan RijnRRZordevanPETilanusHWIndications and long-term outcome of treatment for benign hepatic tumors: a critical appraisalArch Surg20011361033811529826T2-weighted liver MR image shows a hyper-intense mass located in segment IVT1-weighted fat-saturated gradient echo image of the liver shows the heterogeneous mass with high signal intensity (arrow), revealing a recent bleedingSpontaneous rupture hepatocellular adenoma showing areas of hemorrhage and liver parenchyma damageTable 1Surgical management of spontaneous ruptured hepatocellular adenoma (SRHA)nSex/GenderDuration of contraceptive useClinical findingsComputed TomographyTreatmentTumor Size (cm)ComplicationsLength of hospital stay1M/46-Intraperitoneal bleeding + HIHeterogeneous image (hypoattenuating)Right hemihepatectomy (S-V,VI,VII,VIII)10Bile leak25 days2F/383 yearsIntrahepatic bleeding + HIHeterogeneous image (hyperattenuating) + necrosis/ hemorrhageNon-anatomic hepatic resection (S-IVb,V,VI,VII,VIII)12Subphrenic abscess43 days3F/ 415 yearsIntrahepatic bleeding + HIHeterogeneous image (hyperattenuating) + necrosis/hemorrhageExtended right hepatectomy (S-IVa,IVb,V,VI,VII,VIII)13Pleural effusion28 daysM- male; F- female; HI- hemodynamic instability; S-segments.']",Figure 1 T2-weighted liver MR image shows a hyper-intense mass located in segment IV,yes
PMC11618779,Figure_2,oa_package/8d/fc/PMC11618779.tar.gz,"['The tumor was found to encase the right supraclinoid and cavernous segments of the internal carotid artery (ICA), along with the proximal M1 segment with extension to the cavernous sinus [a].', '5 mm right middle cerebral artery bifurcation aneurysm with blebs directed inferiorly and toward the meningioma [b].', 'Given the high complexity of this architecture and the risk of intraoperative aneurysm rupture, a staged approach with preoperative endovascular management of the aneurysm was recommended [c].', 'Postoperative imaging showed complete tumor resection, and a follow-up angiogram 2 months later demonstrated complete aneurysm occlusion [d].', ':Axial (a) magnetic resonance imaging (MRI) view depicting a right sphenoid wing meningioma (white arrow) with extension to the cavernous sinus.']","Figure 2: Axial (a) magnetic resonance imaging (MRI) view depicting a right sphenoid wing meningioma (white arrow) with extension to the cavernous sinus. (b) Digital subtraction angiography view showing an aneurysm at the right middle cerebral artery bifurcation (white arrow). Postoperative angiogram (c) showing resolution of the aneurysm, and (d) MRI showing complete resection of the brain lesion.",yes
PMC10467212,Figure_3,oa_package/0b/d5/PMC10467212.tar.gz,"['No suspicious neoplastic cells were identified ((a c)).', '.', '1177_2050313X231197009-fig3"" position=""float""/>Based on these findings, a diagnosis of scar endometriosis was established, and the patient was initially managed conservatively with analgesics and oral contraceptive pills.']",Figure 3. (a) Low power microscopic view showing a cluster of epithelial cells (green line) and aggregate of stromal cells (black line). (b) High-power microscopic view showing the fragment of endometrial stromal cells. (c) High-power microscopic view showing the cluster of endometrial epithelial cells.,yes
PMC8369722,Figure_1,oa_package/c3/4b/PMC8369722.tar.gz,"['1A).', '1B).', 'Complement inhibitors rescue alpha-synuclein-induced toxicity in SH-SY5Y cells.', '05To investigate if -syn directly can activate the complement system, we used a plate-based immunoassay to investigate if immobilized -syn can stimulate the complement-dependent deposition of complement factor C4b on the surface of -syn-coated microtiter plates.']","Fig. 1 Complement inhibitors rescue alpha-synuclein-induced toxicity in SH-SY5Y cells. Non-mitotic transgenic SH-SY5Y cells expressing -synuclein (-syn) or -galactosidase (-gal) were cultured in 15% normal human serum (NHS) or heat-inactivated human serum (HIS) for 10 days before viability was measured by an MTT assay. Viability was normalized to the control -gal expression cells cultured in NHS. Same as in but cells were cultured in NHS without or in the presence of complement cascade inhibitors RaCI or Cp20. The bars represent the mean SD of three independent experiments. Statistical test: one-way ANOVA followed by Tukeys multiple comparison test. n.s. not significant, * < 0.05",yes
PMC8055530,Figure_2,oa_package/fb/99/PMC8055530.tar.gz,"[') Four weeks following the PET/CT, the patient underwent catheter angiography with a view to image and subsequently treat the complex, large, left shoulder HFAVM.']",Fig. 2 FDG PET/CT performed 60 minutes after injection of 400MBq of 18-F-FDG. This illustrates avid uptake within the region of the left humeral head (blue arrow) (color version of the figure is available online.),yes
PMC4883994,Figure_5,oa_package/f6/9a/PMC4883994.tar.gz,"['Serous retinal detachment, outer retinal changes in form of disruption of IS/OS junction, proliferating retinal pigment epithelium cells [[21]] were well made out in all three scanning techniques, whereas increased choroidal thickness with hyporeflective choroidal granulomas [[22]] in cases of choroiditis [[22]] and outer choroidal border was well made out only in the EDI and CDI scans ( 5).', 'Tuberculosis.', 'In sarcoidosis, localized hyporeflective choroidal thickening in the areas of choroidal granulomas was noticed, and our observation was similar to that by Rostaqui et al.', 'gif"">Authors original file for figure 5Exemplar annotated B-scans showing annotated cysts in green.']",Figure 3 Exemplar annotated B-scans showing annotated cysts in green.,yes
PMC5491586,Figure_1,oa_package/90/db/PMC5491586.tar.gz,"['Grossly, the outer surface of the brain is unremarkable but corpus callosum and lateral geniculate body was diminished in size ().', 'A comparison of high school and collegiate athletesJ Pediatr20031425465531275638811CorsellisJABrutonCJFreeman-BrowneDThe aftermath of boxingPsychol Med19733270303472919112GhajariMHellyerPJSharpDJComputational modelling of traumatic brain injury predicts the location of chronic traumatic encephalopathy pathologyBrain20171403333432804395713MartlandHSPunch drunkJ Am Med Assoc1928911103110714EdwardsG3rdMoreno-GonzalezISotoCAmyloid-beta and tau pathology following repetitive mild traumatic brain injuryBiochem Biophys Res Commun2017483113711422749207015JellingerKAAttemsJNeurofibrillary tangle-predominant dementia: comparison with classical Alzheimer diseaseActa Neuropathol20071131071171708913416MezJSolomonTMDaneshvarDHMurphyLKiernanPTMontenigroPHKriegelJAbdolmohammadiBFryBBabcockKJAdamsJWBourlasAPPapadopoulosZMcHaleLArdaughBMMartinBRDixonDNowinskiCJChaissonCAlvarezVETripodisYSteinTDGoldsteinLEKatzDIKowallNWCantuRCSternRAMcKeeACAssessing clinicopathological correlation in chronic traumatic encephalopathy: rationale and methods for the UNITE studyAlzheimers Res Ther201576226455775(A-D) Grossly, there are no significant lesions on the exterior and cut surfaces of the brain.']","Fig. 1 (A-D) Grossly, there are no significant lesions on the exterior and cut surfaces of the brain. (C) Mamillary and lateral geniculate bodies look atrophic. (C, D) There are slit-like punctate discolorations, here and there, that suggests small bleeding.",yes
PMC4969667,Figure_5,oa_package/59/d6/PMC4969667.tar.gz,"[' 5), the latter supported by rays with a variable layout and different origin: ventral, lateral, externo-dorsal and median lateral.', 'Light microscopy (a) and SEM photomicrographs (b) of copulatory bursa of A.', 'Scale-bars: a, 200 m; b, 50 mSEM micrograph of adult male of A.']","Fig. 5 Light microscopy ( ) and SEM photomicrographs ( ) of copulatory bursa of : left ventro-lateral lobe (a1); right ventro-lateral lobe (a2); dorsal lobe (b); ventral ray (v); ventro-ventral branch of ventral ray (vv); ventro-lateral branch of ventral ray (vl); protuberances (p); lateral ray (l); externo-lateral part of lateral ray (el); medio-lateral branch of lateral ray (ml); postero-lateral branch of lateral ray (pl); externo-dorsal ray (ed); median dorsal ray (md); spicules (s). : a, 200m; b, 50m",yes
PMC10721279,Figure_5,oa_package/c6/4f/PMC10721279.tar.gz,"['Moreover, the N103A mutation of -syn, which blocks AEP-mediated cleavage, also attenuated -syn aggregation induced by -syn PFFs and cholestanol (Supplemental , A and B).', 'No obvious behavioral abnormalities were observed in mice injected with PBS (, A C).', 'Fewer pS129-positive aggregates were found in the CP11 pretreatment group than in the vehicle control group (, D and E).', 'Western blot analysis showed that CP11 attenuated -syn cleavage and hyperphosphorylation and augmented TH and DAT levels compared with the vehicle control (F and Supplemental , A F).', 'Consistently, augmentation of dopaminergic neurons in the substantia nigra and dopaminergic neurites in the striatum was pronounced in mice treated with CP11 (, G J).', 'CP11 ameliorates cholestanol-induced -syn pathology and motor deficits.']","Figure 5 CP11 ameliorates cholestanol-induced -syn pathology and motor deficits. The mice were injected with CP11 i.p. or vehicle control, fed a cholestanol diet, and then were stereotaxically injected with -syn PFFs or PBS. ( ) Rotarod test ( = 6). ( ) Pole test ( = 6). ( ) Beam-walking test ( = 6). ( ) Representative pS129 immunostaining in the cortex, striatum, substantia nigra, and hippocampus of mice sacrificed 6 months after intrastriatal -syn PFF or PBS injection. The histogram shows the percentage of neurons containing -syn aggregates ( = 4 mice/group). Scale bar: 20 m. ( ) Double immunostaining of pS129 and TH in the substantia nigra. The histogram shows the ratio of the pS129 area to the TH area ( = 4 mice/group). Scale bar: 20 m. ( ) Western blot analysis of p--syn, total -syn, TH, and DAT ( = 3 mice/group). ( and ) Representative images of TH-positive fibers in the striatum. The histogram shows the density of TH-positive fibers in the striatum ( = 5 mice per group). Scale bar: 200 m. ( and ) Representative images of TH-positive neurons in the SNpc. The histogram shows the number of TH-positive neurons in the SNpc ( = 4 mice/group). Scale bar: 100 m. Data are presented as mean SD. * < 0.05, ** < 0.01, *** < 0.005, **** < 0.001; compared by 1-way ANOVA with Tukeys multiple comparison test. Ctx, cortex; ST, striatum; SN, substantia nigra; Hip, hippocampus.",yes
PMC8637002,Figure_2,oa_package/82/3c/PMC8637002.tar.gz,['The patient underwent a right parietal-occipital craniotomy with gross total resection.'],"Fig. 2 MRI of the brain. (A, B): DWI and ADC map. Mass in the right parieto-occipital region demonstrates peripheral rim of faint increased signal on DWI with subtle signal hypointensity on ADC map consistent with diffusion restriction. (C): T2 weighted image demonstrates central heterogenous T2 hypointensity. (D): Fat suppressed thin slice contrast enhanced T1 weighted image demonstrates thick, nodular rim of peripheral enhancement in the corresponding location of the diffusion restriction.",yes
PMC4214685,Figure_1,oa_package/c8/44/PMC4214685.tar.gz,"['Rats in the normal group showed rare hepatic iron and steatosis (a and 1b).', ""In the fatty liver group, many hepatocytes with lipid droplets could be found with HE staining (c), while the cells were negative for Perls' Prussian blue staining (d)."", 'Both lipid droplets and blue dots were observed in the coexisting group (e and 1f).', ""In the liver iron group, the HE staining was negative (g), but there were many blue dots with Perls' Prussian blue staining (h)."", 'g001The rats were grouped by their liver histological results.']",10.1371/journal.pone.0110964.g001,yes
PMC9675370,Figure_1,oa_package/c7/20/PMC9675370.tar.gz,[],FIGURE 1 MRI of the lumbar spine showing enhancing soft tissue mass at the L4/5 vertebra extending into the spinal canal with compression of the thecal sac (red arrow).,yes
PMC11403430,Figure_4,oa_package/86/f8/PMC11403430.tar.gz,"['As shown in , the lesion contained material hypointense to urine in the inferior pole of the right kidney, though it had rapidly increased in size during admission consistent with an abscess.', 'Postprocedure kidney ultrasound, , showed improvement of the right renal lesion after drainage with mild residual hydronephrosis and worsening left-sided hydronephrosis, which was likely secondary to physiological changes in pregnancy [9].']",Fig. 4 Coronal T2 HASTE FS MBH MRI highlights the presence of material hypointense to urine.,yes
PMC11622091,Figure_5,oa_package/e9/90/PMC11622091.tar.gz,"['\nHematoxylin and eosin staining of bile duct and liver tissues in the two groups (magnification, 4 ).']",Figure 5 A: Healthy control group; B: Model group.,yes
PMC6639202,Figure_2,oa_package/82/69/PMC6639202.tar.gz,"['056, respectively) ().', '.']",Fig. 2. Kaplan-Meier survival curves according to mucin proportion in pretreatment magnetic resonance imaging (MRI). (A) Overall survival. (B) Disease-free survival.,yes
PMC6214601,Figure_2,oa_package/eb/83/PMC6214601.tar.gz,"['Radiographic evidence indicated osteolytic epiphyses, with the presence of the Codman triangle, which is a primary characteristic of OSA observed in bone tissue (A).', 'Further, erosion of the affected skeleton deteriorated over time (B).', 'Skeleton of the affected limbs was severely eroded, and the affected soft tissue formed few lumps with calcification shadows 28 days later (C).', 'With progression in time, the proximal tibia tissue was completely impaired, and the adjacent soft lumps continued to grow until 42 days (D).', 'Histopathological appearance of lung metastasis nodules.']","Figure 2 Radiographs indicated the impaired proximal tibias by OSA. ( ) Thirteen days after the inoculation of tumor cells, tumor lesion in the injected proximal tibia was noticed, with osteolysis indicated by the Codman triangle (red arrow). Erosion of the affected skeleton deteriorated over time ( ), and the surrounding soft tissue started to form lumps with calcification shadows 28 days postinnocuation ( ). ( ) The tumor lesion became completely destructive at the injected tibia at 42 days. OSA, osteosarcoma.",yes
PMC10493587,Figure_4,oa_package/09/4a/PMC10493587.tar.gz,"['NF B signaling pathway is an essential downstream for GAS6/AXL functioning\nThe scatterplot of enriched KEGG pathways statistics was made.', 'Source Data for .', 'It revealed no recurrent/residual mass lesion in urinary bladder (d).']","Figure 2. 46-year-old-female with haematuria. Coronal and Sagittal reconstructions of urinary bladder in venous phase (a, b) shows filling defect in urinary bladder containing calcific foci. There is minimal thickening of bladder wall on left side. Axial image at the level of kidneys show mild dilatation of right renal pelvis (arrow). 6 month follow-up sonogram (d) of urinary bladder shows normal lumen and contours of urinary bladder.",yes
PMC5563017,Figure_3,oa_package/a1/7c/PMC5563017.tar.gz,['Reproduced with permission from Chest Atlas\n\nThe electronic survey was piloted by 3 radiology residents and 6 medical students under the same conditions as the main study.'],"Fig. 3 A 29year-old man with severe respiratory distress and hypotension in the setting of persistent left hemithorax pain following a cough attack. The CXR demonstrates loss of pulmonary vascular markings on the left side, leading to its remarkable left hemithoracic translucency associated with left pulmonary total collapse and rightward mediastinal shift. Tension pneumothorax. Available at: . Reproduced with permission from",yes
PMC4504064,Figure_3,oa_package/49/18/PMC4504064.tar.gz,"[' 3).', 'The types of follicular cancer are discriminatedDiscussionIodine is broadly distributed in the environment in the form of iodide [1] and is concentrated in sea water and in marine life [5].']",Fig. 3 Histological diagnosis (illustrative display)Thyroid malignanciesJohannesburg: 5year period (January 1984December 1988). The types of follicular cancer are discriminated,yes
PMC10730449,Figure_2,oa_package/b5/cb/PMC10730449.tar.gz,"['9,10 Multiphase CT or MRI are often needed for the diagnosis demonstrating an intraluminal mass within the IVC, indistinguishable on imaging from other IVC neoplasms ().']",FIGURE 2 A 36-year-old female with acute abdominal pain related to cholelithiasis and an incidental inferior vena cava (IVC) finding on upper abdominal ultrasound. (a) Greyscale ultrasound image showed a 1.5 cm hypoechoic lesion (circle) within the IVC lumen. (b) The colour Doppler image shows no significant internal flow within the lesion (circle). (c) Contrast-enhanced CT of the abdomen in the portal venous phase did not reveal significant enhancement (arrowhead). (d) Axial T2-weighted images of the abdomen show hyperintense signal within the IVC (arrowhead). Dynamic contrast-enhanced MRI (e) portal venous phase and (f) delayed sequence acquired at 5 min after the injection demonstrate progressive enhancement with centripetal fill in suggesting the diagnosis of an IVC haemangioma (arrowhead).,yes
PMC8756316,Figure_4,oa_package/38/b7/PMC8756316.tar.gz,['Pathological changes in islets in type 2 diabetes patients with acute myocardial infarction.'],"Figure 4 Pathological changes in islets in type2 diabetes patients with acute myocardial infarction. Type2 diabetes complicated with acute myocardial infarction shows amyloid deposition, thickening of the basement membrane and loss of pericyte coverage of microvessels in the islets. These changes are similar to those in microvascular complications of diabetes.",yes
PMC6206697,Figure_3,oa_package/78/ac/PMC6206697.tar.gz,['.'],Fig. 3. ( ) An irreparable labrum demonstrating complex tearing and degeneration. ( ) Determining the length of the defect intraoperatively (effectively the chord length multiplied by 1.3). ( ) Completed labral reconstruction.,yes
PMC6851499,Figure_3,oa_package/1b/3b/PMC6851499.tar.gz,"[' 3a c).', '', ' 5b in Online Resource 1, respectivelyIn the experiments described thus far PFFs were delivered as naked protein, probably entering the cell via an endocytic pathway.', ' 3d).', ' 3e, f; Supplementary ', ' 3f; Supplementary ']","Fig.3 Intracellular tau pathology causes GVB formation. Primary mouse neurons with (+) or without () expression of tau-P301L in the presence (PFF) or absence (Ctrl) of seeds. Representative epifluorescence images of immunostaining with AT100 (green), the GVB marker pPERK (red) and the neuron-specific dendritic marker MAP2 (gray) are shown. Image acquisition and analysis were automated. Arrowheads point to examples of neurons with GVBs. , Quantification of the conditions shown in . Tau pathology load ( ) and GVB load ( ) were quantified as described in the and are presented as normalized background corrected values. Bars indicate the mean of the observations+SEM. =3 independent experiments, =16 observations per condition, on average 6101 neurons per observation. *** <0.001, not significant, tau pathology load: one-way ANOVA followed by Tukeys multiple comparison test, GVB load: KruskalWallis test followed by Dunns multiple comparison test. Representative confocal images of tau-P301L-GFP (green) expressing neurons immunostained with the GVB marker CK1 (red) that were exposed to PFFs either by direct addition to the culture medium (Naked) or by Lipofectamine 2000-mediated transduction (Lipofection). See Supplementary Table3 in Online Resource 1 for an overview of the number of cells analyzed. , Neurons expressing tau-P301L-GFP (green) were treated with 40, 80 or 160nM PFFs or control buffer (Ctrl). At the endpoint of the experiment soluble tau was extracted using ice-cold MeOH. Image acquisition and analysis were automated. Representative example showing 24 representative epifluorescence images per condition. Quantification of the tau pathology and GVB load in the conditions shown in and measured as described in the . Data points represent average of =1618 observations from =3 independent experiments, on average 7747 neurons per observation. *** <0.001, Pearson =0.87. Linear regression line is shown in the graph and inset shows and value. Cell nuclei are stained with DAPI (blue) in all images. Quantification of cell number in the conditions shown in and are shown in Supplementary Fig.3g and Supplementary Fig.5b in Online Resource 1, respectively",yes
PMC9587204,Figure_1,oa_package/58/e6/PMC9587204.tar.gz,"[' 1 shows examples of OCT images before and after binarization.', 'An example of OCT image before and after binarizationStatistical AnalysisCVI and subfoveal CT in patients with BVMD and healthy controls were compared using independent sample t test.']",Fig. 1 An example of OCT image before and after binarization,yes
PMC10721145,Figure_4,oa_package/e6/5a/PMC10721145.tar.gz,"['Nur77GFP mice developed increased LV weight in response to H/L, but this was independent of active CD4+ TCR engagement at this time point, reflected by a similar frequency of CD4+GFP+ cardiac T cells in H/L mice and STD controls, and a significantly lower frequency than in mice subjected to TAC (, A and B, and Supplemental , A and B).', 'OTII mice fed H/L manifested preserved ejection fraction (Supplemental Table 3), and a striking increase of cardiac CD4+ T cell infiltration compared with STD-fed OTII mice (, C and D, and Supplemental C).', 'This global UPR downregulation was not observed in splenic CD4+ T cells from mice undergoing TAC (, E H) nor in mice single-treated with either l-NAME or HFD only, in which we did not observe total Xbp1, Atf4, or Atf6 downregulation, whereas Xbp1s was downregulated, but to a significantly lesser extent than with the combination of both stressors, which synergistically decreased T cell Xbp1s expression (Supplemental J).', 'Moreover, the expression of Xbp1s, total Xbp1, Atf6, and Atf4 remained unchanged in circulating CD3+ T cells from sham and TAC mice, demonstrating that downregulation of circulating T cell Atf4 is HFpEF specific (, I L).', 'Because we observed downregulated expression of Xbp1s, Atf4, and Atf6 in CD4+ T cells after 5 weeks of H/L (, E H), we next investigated the upstream activation states of IRE1 , PERK, and ATF6.', 'We corroborated specific silencing of the gene coding for Xbp1 in CD4+ T cells at the genome level, and the inability to activate XBP1s protein expression by the ER stressor tunicamycin in T cells from T-Xbp1KO mice (Supplemental , A C).', 'Experimental HFpEF imprints a T cell immune signature distinct to experimental HFpEF.']","Figure 4 Experimental HFpEF imprints a T cell immune signature distinct to experimental HFpEF. ( and ) CD45 CD4 GFP cardiac cells were analyzed in STD, H/L, and TAC surgery Nur77 mice 5 weeks after respective treatment. ( and ) Cardiac CD45 CD3 CD4 cells were measured directly by flow cytometry in OTII mice fed H/L or STD for 5 weeks. ( ) Splenic CD4 ( ) or blood CD3 ( ) T cells were isolated from WT mice fed H/L or STD (HFpEF), or from WT mice given TAC or sham surgery (HFrEF), and analyzed for the gene expression of , total , , and by qPCR. = 311. : Each replicate is = 2 mice pooled. Error bars represent the mean SEM. : One-way ANOVA with Tukeys multiple-comparison test; : unpaired, 2-tailed test; : HF groups (H/L or TAC) were compared against their respective controls (STD and sham, respectively) in the unpaired, 2-tailed test. * 0.05, ** 0.01, *** 0.001. This figure was created using Biorender.com.",yes
PMC10808633,Figure_2,oa_package/61/62/PMC10808633.tar.gz,['3D time-of-flight (TOF) magnetic resonance (MR) angiography slices highlighting the involved efferent venous pathways of the sphenoid wing (SW)-DAVF in this case.'],"Figure 2 3D time-of-flight (TOF) magnetic resonance (MR) angiography slices highlighting the involved efferent venous pathways of the sphenoid wing (SW)-DAVF in this case. Axial (1), coronal (2), and sagittal (3) slices depict the superior ophthalmic vein (SOV) as an efferent pathway, denoted by yellow arrows. Axial (1), coronal (2), and sagittal (3) slices illustrate the inferior ophthalmic vein as a potential efferent pathway, indicated by blue arrows. Axial (1), coronal (2), and sagittal (3) slices display the pterygoid plexus, marked with red arrows.",yes
PMC4165592,Figure_5,oa_package/56/10/PMC4165592.tar.gz,"['Numbers of adult worms in the small intestine of DR3-deficient mice were lower at both the peak of infection at day 6 or at day 14 compared to wild-type mice, with corresponding reductions in the number of eggs in the stool (c), indicating highly effective immunity to N.', 'brasiliensis, with more sustained elevation in IL-13 mRNA (d).', 'Furthermore, systemic levels of IL-13 protein and its secreted receptor, IL-13R 2, a marker of IL-13 bioactivity, also increased in infected DR3 deficient mice with similar kinetics and with equal saturation of IL-13R 2 by IL-13 (e).', 'brasiliensis, (f) suggesting that helminth-driven expansion of ILC2 is independent of DR3.', 'See also S3DR3 is not required for IL-13 production and clearance of parasites in Nippostrongylus brasiliensis infection(a) Representative PAS-stained duodenum sections from wild-type or DR3 / mice without or 6 days post infection (DPI) with Nippostrongylus brasiliensis.']","Figure 5 DR3 is not required for IL-13 production and clearance of parasites in infection (a) Representative PAS-stained duodenum sections from wild-type or DR3 mice without or 6 days post infection (DPI) with Scale bar=100m. (b) Mucosal thickness and goblet cell area in these samples are shown for 49 mice per group with each dot representing the average within a group. Data represent mean SEM, analyzed with Mann Whitney (*p<0.05, ***p<0.001). (c) Adult worm counts from the intestine at 6 and 14 days post infection, and counts of eggs in the stool over the course of infection. Data represent mean SEM. (d) IL-13 mRNA expression in the duodenum over the course of infection normalized to 2m expression and shown relative to expression levels in untreated WT mice with mean SEM. (e) Serum levels of IL-13R2 (white bars) and IL-13 bound to IL-13R2 (grey bars) measured in serum at the indicated times after infection in the indicated mice. (f) ILC2 quantitated in mesenteric lymph nodes of uninfected and 6DPI infected mice using Lin (CD3CD4CD8CD11bCD19MHCIIFcRI) ckit Sca1 as markers of ILC2. See also .",yes
PMC3232538,Figure_5,oa_package/02/26/PMC3232538.tar.gz,['Animation as a series of still framesWe will now retain only those frames that are of interest.'],Figure 5 Animation as a series of still frames,yes
PMC6829593,Figure_8,oa_package/85/28/PMC6829593.tar.gz,"['CBC results showed that PLX3397 treatment significantly reduced the number of RBCs (RBC and hematocrit %) and hemoglobin levels (hemoglobin and mean corpuscular hemoglobin concentration), while increasing the size of RBCs (mean corpuscular volume) in all groups ( A).', 'The number of platelets was also reduced ( B).', 'Interestingly, PLX3397 treatment reduced the total number of white blood cells in E4 and TE4 mice, but not in EKO and TEKO mice ( C), suggesting an apoE-dependent effect on white blood cells under PLX3397 treatment.', 'S5) revealed that PLX3397 treatment reduced or showed a trend of reducing the frequency of dendritic cells (DCs), natural killer (NK) cells, and Ly6C monocytes in all groups of mice (, D F).', 'PLX3397 preferentially targeted Ly6C monocytes over Ly6C+ monocytes, including Ly6Chi and Ly6Clo monocytes ( F).', 'We found that in control chow-treated mice, Ly6C+ monocytes expressed a significantly higher level of CSF1R (CD115) compared with Ly6C monocytes ( G), suggesting a higher tolerance to CSF1R inhibition-induced cell death by Ly6C+ monocytes.', 'Interestingly, PLX3397 treatment led to a notable reduction of CSF1R expression in Ly6C+ monocytes and a milder CSF1R reduction in Ly6C monocytes, with all surviving monocyte populations expressing a similarly low level of CSF1R ( G).', 'On the other hand, there appeared to be an overall trend of increased T cell frequency ( H) upon PLX3397 treatment.', 'This effect was most pronounced in TEKO mice and was observed in all the T cell subpopulations, including regulatory T cells (T reg cells; I), CD4+ T cells, CD8+ T cells, memory T cells, and NK T cells (other data not shown).', 'In addition, there was a trend of increased B cell frequency in control TE4 mice over other groups ( J).', '.', 'DiscussionAD and many other neurodegenerative diseases are known as proteopathies characterized by accumulation of misfolded and aggregated proteins.']","Figure 8. Complete blood cell count results for the level of RBCs, hemoglobin, platelets, and white blood cells in 9.5-mo-old mice of all genotypes treated with control (Ctl) or PLX3397-supplemented (PLX) chow (TE4-Ctl: = 14; TE4-PLX: = 7; TEKO-Ctl: = 11; TEKO-PLX: = 10; E4-Ctl: = 13; E4-PLX: = 8; EKO-Ctl: = 10; EKO-PLX: = 7). Flow cytometry quantification of the frequency of DCs, NK cells, Ly6C , Ly6C , and Ly6C monocytes, respectively (TE4-Ctl: = 7; TE4-PLX: = 7; TEKO-Ctl: = 7; TEKO-PLX: = 5; E4-Ctl: = 11; E4-PLX: = 8; EKO-Ctl: = 5; EKO-PLX: = 5). Median fluorescence intensity (MFI) of CSF1R (CD115) in Ly6C , Ly6C , and Ly6C monocytes in all groups of mice (two-way ANOVA with Sidaks post hoc test). Population frequencies for T cells, T reg cells, and B cells, respectively. Data are expressed as means SEM. One-way ANOVA with Tukeys post hoc test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. HCT, hematocrit; HGB, hemoglobin; MCHC, mean corpuscular hemoglobin concentration; MCV, mean corpuscular volume . The CBC and flow cytometry experiments for blood cell phenotyping were performed >10 times.",yes
PMC3419093,Figure_3,oa_package/b8/c4/PMC3419093.tar.gz,[' The follow-up lateral radiograph shows good healing of the C2 vertebral body; the prevertebral soft tissue shadow has also returned to normal.'],Figure 3 The follow-up lateral radiograph shows good healing of the C2 vertebral body; the prevertebral soft tissue shadow has also returned to normal.,yes
PMC3782460,Figure_4,oa_package/d5/63/PMC3782460.tar.gz,"['We observed that STAg-pretreated 5-LO-/- mice were resistant to infection compared with PBS-pretreated 5-LO-/- mice (A).', 'gondii infection (B-4D).', 'g004Mortality rates of 5 LO-/-, IL-4-/-, IL-12p40-/-, MyD88-/- and CCR5-/- STAg-pretreated and infected mice.', 'We observed that STAg-pretreated IL-4-/- mice efficiently controlled the infection (E).']",10.1371/journal.pone.0075138.g004,yes
PMC9476256,Figure_1,oa_package/18/da/PMC9476256.tar.gz,"[' 1).', 'Cohort characteristics.', 'Scale bars represent 50 mPosterior fossa tumors with ependymal/subependymal features, H3 K27M-positive encompass different subgroups with distinct clinical outcomesWe subjected these nine H3 K27M EPN_PFA cases to DNA-methylation profiling (with the v12.', 'pdf"">Additional file 1: .']","Fig. 1 Cohort characteristics. Based on H3K27me3 immunohistochemistry, 116 cases were classified as EPN_PFA showing a loss of immunoexpression, and 31 were EPN_PFB with a conserved H3K27me3 expression. From the 116 EPN_PFA, 108 were immunopositive for EZHIP and eight cases were immunopositive for the H3K27M mutation. From the national French Neuropathological Reference network (RENOCLIP-LOC) database, we found one additional pediatric case of EPN_PFA with H3K27M immunopositivity. Scale bars represent 50m",yes
PMC11467228,Figure_2,oa_package/81/b3/PMC11467228.tar.gz,['MP2RAGE post-processing techniques edge enhanced (left) and edge only (right) reveal marked contrast differentiation between grey and white matter regions.'],"Figure 2 MP2RAGE post-processing techniquesedge enhanced (left) and edge only (right)reveal marked contrast differentiation between grey and white matter regions. Arrows highlight the FCD (same patient as ). MP2RAGE, magnetization prepared 2 rapid gradient echo; FCD, focal cortical dysplasia.",yes
PMC9689876,Figure_1,oa_package/0d/21/PMC9689876.tar.gz,"['Diffuse bone sclerosis can be found in all subtypes of osteopetrosis (ADO, ARO, IAO), even if with slightly different patterns and skeletal locations; particularly in the ARO type, the bone sclerosis can be so marked to assume the so-called marble bone appearance ().', '2174/157340561766621012911133933511927(A) conventional radiography of the chest of a young healthy female (shown as comparison).']","Figure 1 ( ) conventional radiography of the chest of a young healthy female (shown as comparison). ( ) conventional radiography of the chest in a 29-year-old female diagnosed with osteopetrosis (clinical and radiological diagnosis in absence of a pathogenic variant in osteopetrosis-related genes). All the skeletal segments included are affected by diffuse and markedly increased bone density (spine, ribs, clavicle, scapulae, humerus): Marble bone appearance.",yes
PMC3334819,Figure_3,oa_package/c7/c3/PMC3334819.tar.gz,"['In HeLa cells, ClC-2 transfected alone was detected at the plasma membrane and intracellularly ( 3A).', 'Coexpression with GlialCAM directed the ClC-2 channel to cell-cell contacts (s 3B 3D), where both proteins colocalized (data not shown).', 'Localization of ClC-2 together with GlialCAM was observed in long ( 3B) or short ( 3C) cell-cell contact processes and in extensive contact areas between opposite cells ( 3D).', 'Such a clustering was never observed in contacting cells expressing only ClC-2 ( 3A).', 'In these cultures, adenoviral-mediated expression of ClC-2 with or without GlialCAM showed that the latter protein was necessary to target ClC-2 to astrocyte-astrocyte processes (compare s 3E and 3F).', 'In these junctions, ClC-2 and GlialCAM displayed colocalization (s 3F 3H).', ' 3GlialCAM Changes the Subcellular Distribution of ClC-2 in HeLa Cells and in Primary Cultures of Astrocytes(A D) GlialCAM changed the subcellular distribution of ClC-2 in transiently transfected HeLa cells from being at the plasma membrane when transfected alone (A) versus being at long cell-cell contact processes (B), at short cell-cell contact processes (C), or in extensive contact regions (D) when cotransfected with GlialCAM (arrows label cell-cell contacts).']","Figure3 GlialCAM Changes the Subcellular Distribution of ClC-2 in HeLa Cells and in Primary Cultures of Astrocytes (AD) GlialCAM changed the subcellular distribution of ClC-2 in transiently transfected HeLa cells from being at the plasma membrane when transfected alone (A) versus being at long cell-cell contact processes (B), at short cell-cell contact processes (C), or in extensive contact regions (D) when cotransfected with GlialCAM (arrows label cell-cell contacts). Scale bar: 10m. (EH) Astrocytes were transduced with adenoviruses expressing ClC-2 alone or together with C terminally tagged GlialCAM at MOI 3. GlialCAM similarly brought ClC-2 to cell-cell contacts. Arrows point to astrocyte-astrocyte contacts. Immunofluorescence used a flag monoclonal antibody detecting GlialCAM protein (red) or a rabbit polyclonal antibody (C1) detecting ClC-2 (green). Colocalization between the red and the green fluorescence results in a yellow coloring (Merge). Nuclei of astrocytes were stained using DAPI (blue). Scale bar: 20m. See also .",yes
PMC3323572,Figure_1,oa_package/af/3f/PMC3323572.tar.gz,"['236), (A).', '2) with the lowest levels measured in TAO patients (B).', '024), similarly to those of nonsmokers (C).', '008) (D).', '18 106 PBMCs per ml blood) (E).', 'g001Levels of circulating progenitor cells and PBMCs in TAO patients.']",10.1371/journal.pone.0034717.g001,yes
PMC6169688,Figure_5,oa_package/14/06/PMC6169688.tar.gz,"['Furthermore, fluorescence in situ hybridisation (FISH) molecular analysis demonstrated amplification of the murine double minute 2 (MDM2) oncogene, supporting the diagnosis of undifferentiated sarcoma (figure 5).', ""Dual colour fluorescence in situ hybridisation with probes specific for the murine double minute 2 (MDM2) locus at 12p15 demonstrating amplification of the orange (3' MDM2) signal relative to the control green chromosome enumeration probe (CEP) at a ratio of 2.""]",Figure 3 Gross photograph highlighting a 4.54.02.2cm left atrial mass overlying right fibrous trigone of mitral valve.,yes
PMC5239448,Figure_4,oa_package/1c/71/PMC5239448.tar.gz,"['Epsin2 depletion disrupts actin cap formation and polar body extrusion in oocytesMII oocytes were labeled with phalloidin to visualize actin (green), and counterstained with Hoechst 33342 for chromosomes (blue).']","Figure 4 Epsin2 depletion disrupts actin cap formation and polar body extrusion in oocytes MII oocytes were labeled with phalloidin to visualize actin (green), and counterstained with Hoechst 33342 for chromosomes (blue). Representative confocal sections showing the actin distribution in control (a), Epsin2-KD (d), and Epsin2-siRNA+cRNA (g) oocytes. Arrowhead denotes the position of actin cap. Right graphs show the fluorescence intensity profiling of phalloidin in oocytes. Lines were drawn through the oocytes, and pixel intensities were quantified along the lines. , Quantitative analysis of control ( = 150), Epsin2-KD ( = 122), and Epsin2-siRNA+cRNA oocytes ( = 138) with intact actin cap and extruded polar body. Data are presented as mean SD from three independent experiments. * < 0.05 . controls. Scale bar, 20m.",yes
PMC9769954,Figure_2,oa_package/29/19/PMC9769954.tar.gz,"['5 cm pedunculated lesion on the midline of the tongue ().', 'Flexible nasendoscopy image of the pedunculated RCC on the dorsum of the tongue.']",Figure 2 Flexible nasendoscopy image of the pedunculated RCC on the dorsum of the tongue.,yes
PMC4230233,Figure_7,oa_package/f0/92/PMC4230233.tar.gz,"['Comparison of cell surface markers and inflammatory factor expression in M0, M1 and M2 macrophages.']","Figure 7 Cell surface marker staining of F4/80 and CD206 expression in M0, M1 and M2 macrophages. RAW264.7 macrophages cultured in normal medium were defined as M0 with significant F4/80 and negative CD206 expression. RAW264.7 macrophages stimulated with LPS were defined as M1 with significant F4/80 and weak CD206 expression. RAW264.7 macrophages stimulated with LPS and co-cultured with MSCs in transwells were defined as M2 with significant expression of F4/80 and CD206. ELISA showed that MSC treatment significantly decreased the levels of the pro-inflammatory cytokines TNF- and IL-6 and increased the levels of the anti-inflammatory cytokine IL-10 in culture medium. * <0.05 versus M0, <0.05 versus M2, n=3. MSCs increased the percentage of CD206 IL-10 cells in RAW264.7 macrophages after co-culture for 72hours. <0.05 versus M0 or M1, n=3. MSCs, mesenchymal stem cells.",yes
PMC11004415,Figure_2,oa_package/5a/18/PMC11004415.tar.gz,"['The VOI was carefully positioned at the center of the right (A) and left (', 'Similarly, the vermis VOI was placed at the center of the vermis (C).', 'Positioning of Magnetic Resonance Spectroscopy (MRS) Voxel of Interest (VOI).', 'Brenner tumour with mucinous portion.']","Figure 6 Brenner tumour with mucinous portion. Collision tumour. Axial T2- (a) and T1-weighted (b) images demonstrate an adnexal multilocular cystic mass with thin septations and a solid posterior hypointense portion (a, arrow). The cystic portion presents multiple loculi with different contents characterized by different signal intensity. The septations and the solid portion present an homogeneous and delayed enhancement on 3D fat-saturated T1-weighted Spoiled Gradient Echo images (c, arrow). The interface between the solid and the cystic mass is regular.",yes
PMC7235566,Figure_3,oa_package/c4/6e/PMC7235566.tar.gz,"['2 cm (, column A).', 'Repeat diagnostic CT of the chest was performed on the 16th day after symptom onset, demonstrating improvement in bilateral, peripheral GGOs with decreased lymphadenopathy, suggesting an amelioration (, column B).', ' 3Left panel: diagnostic computed tomography scan obtained the 13th day after symptom onset demonstrating bilateral peripheral ground-glass opacities, more prominent on the right side.', 'Based on our patient s available imaging, her CT lung abnormalities appeared to peak in severity approximately 11 days after symptom onset, followed by gradual resolution of GGOs as demonstrated in her diagnostic CT scans ().']","Figure3 Left panel: diagnostic computed tomography scan obtained the 13th day after symptom onset demonstrating bilateral peripheral ground-glass opacities, more prominent on the right side. Right panel: diagnostic computed tomography scan obtained the 16th day after symptom onset, with improvement and diminished volume of ground-glass opacities.",yes
PMC10500936,Figure_3,oa_package/e3/58/PMC10500936.tar.gz,"[' 3A C; Supplementary ', ' 3A C).', 'Effect of LDLc and HDLc supplementation on proliferation, cell cycle, and changes in signaling intermediate in colon cancer cells.', 'The experiment was performed twiceAs the treatment of LDLc or HDLc promotes cell proliferation, the distribution of cells in various phases of the cell cycle with or without LDLc or HDLc treatment was also analyzed in HCT-116 cells.', ' 3D).', ' 3E).', ' 3F; Supplementary ', ' Supplementary figure 3.']","Fig. 3 Effect of LDLc and HDLc supplementation on proliferation, cell cycle, and changes in signaling intermediate in colon cancer cells. HCT-116 cells were cultured in different concentrations of LDLc or HDLc, for different time points thereafter colony formation, cell cycle, and immunoblot analysis were performed as per the protocol mentioned in the methods. Images of the colony were taken in a10 phase contrast microscope on the 12th day before staining with 0.05% crystal. , Images showing crystal violet-stained colonies of HCT-116 cells with or without LDLc, and HDLc treatment along with a bar graph representing the percent colony formed with respect to untreated controls. Colonies were stained with 0.05% crystal violet and images were captured in an Olympus DSLR camera and quantification was done using ImageJ software. Experiments were done in triplicate and performed twice. , Cell cycle analysis of HCT-116 cells treated with or without 50g/ml LDLc or HDLc for 48h. Histogram showing different phases of the cell cycle, and bar graphs representing % cell population in different phases of the cell cycle, and the ratio of Go-G1/S-phase cell population with or without LDLc or HDLc treatment. Experiments were performed twice. Immunoblot analysis of pERK and ERK protein expression was evaluated from the whole cell lysate of HCT-116 cells after treatment with different concentrations of LDLc or HDLc or vehicle control. Tubulin was used as a loading control. The experiment was performed twice",yes
PMC6076361,Figure_5,oa_package/50/63/PMC6076361.tar.gz,"['In the majority of the mice (seven of ten), GFP-positive cells could be detected partway along the rostrocaudal axis, with cells detected in the ventral and/or ventrolateral forebrain, along the fiber tracts of the corpus callosum, and the substantia nigra, as shown in 5A, which shows a mouse with a high level of engraftment.', ' 5NAGLU Corrected N-iNSCs Grafting and Characterization of Enzyme Activity at 9 Months(A) Immunohistological staining of 40- m representative sections for GFP, shown at low magnification, following PAR injection of N-iNSCs into Naglu / mice.', 'Enzyme activity for the corresponding right hemisphere was evaluated for the six rostrocaudal 2-mm slices, as shown in 5B.', 'As expected, no NAGLU activity was detected in vehicle-injected Naglu / mice ( 5C).', 'In the pool of sections 1 and 2, the averaged NAGLU activity in engrafted Naglu / mice reached a level almost 5-fold above the level of unaffected mice ( 5C).', '0017 for sections 5 and 6) and reached approximately 10% of unaffected mice ( 5C).', '32, 33 As expected, hexosaminidase was elevated in vehicle-injected Naglu / mice compared with unaffected mice in all sections ( 5D).', '0001), but no difference was observed for sections 5 and 6, which are farther away from the site of graft placement ( 5D).']","Figure5 NAGLU Corrected N-iNSCs Grafting and Characterization of Enzyme Activity at 9 Months (A) Immunohistological staining of 40-m representative sections for GFP, shown at low magnification, following PAR injection of iNSCs into mice. Post-acquisition processing included adjustments to brightness and contrast and RGB curves using Adobe Photoshop CS6 to improve visibility and consistency in color tone. Scale bar, 1mm. (B) Schematic diagram depicting how the brains were divided. Brains were dissected sagittally along the midline first. One brain hemisphere was for histology, and the other brain hemisphere was further sectioned into 2-mm-thick coronal slices in each animal. For NAGLU and hexosaminidase (HEX) activity assays, brain sections 1 and 2 and 5 and 6 were pooled. The blue dots represent the approximate intraparenchymal injection sites. (C and D) Enzymatic activity of NAGLU (C) and HEX (D), respectively, in mice with iNSCs (/ treated) or saline (/ vehicle) compared with heterozygous control mice (unaffected). All bars show mean SD (n= 1012 mice per group). Mean with SD. Two-tailed, unpaired parametric t tests were used between (/ treated) or saline (/ vehicle) groups.*p< 0.05, p< 0.01, p< 0.0001.",yes
PMC5956015,Figure_3,oa_package/1d/84/PMC5956015.tar.gz,"[' 3).', '', 'Sz-treated rats showed clear evidence of type I diabetes mellitus at week 10 (e and f) but this effect was diminished at week 28 (g and h)\nAnalysis of Cancellous BoneEven though the rats fed a HFD had slightly longer tibias at 10 weeks, geometry of the long bones was similar between rats in all the study groups.']","Fig.3 Examples of exocrine (acini) and endocrine (islets of Langerhans) pancreas from Control ( ), HFD ( ), and HFD/Sz ( ) rats at week10 of the study. Arrows indicate apoptotic -cells and scar tissue in the pancreas of Sz-treated rats ( ). Effect of Sz on pancreas is also clearly visible by immunohistochemistry ( ) with insulin-stained -cells (dark brown) and occasional presence of scar tissue replacing apoptotic -cells indicated by red arrows. For determination of insulin and glucose levels, the rats were fasted overnight, bled the next morning to collect baseline serum, and then dosed with water (Controls) or sugar water (HFD and HFD/Sz). Serum was collected at various intervals for determination of insulin and glucose. Sz-treated rats showed clear evidence of type I diabetes mellitus at week 10 ( and ) but this effect was diminished at week 28 ( and )",yes
PMC4789826,Figure_1,oa_package/0b/d9/PMC4789826.tar.gz,['These estimations are used to perform 3D image reconstruction of the colon lumen and outer wall (figure 1).'],Figure1 Image acquisition by the capsule. Top: Data from Compton backscattering and X-ray fluorescence are combined to estimate the distance from the capsule to the colon walls. Bottom left: Thee-dimensional (3D) reconstruction of the lumen is based on these estimates. Bottom middle: The capsule positioning system presents position and orientation of the capsule to assist in on-line evaluation. Bottom right: Surface and pathway data are fused to create an image reconstruction of the colon.,yes
PMC5296625,Figure_1,oa_package/5c/06/PMC5296625.tar.gz,['A 42-year-old G2P1001 presented for a routine growth ultrasound at 36 wk 5 d.'],"Figure 1 A 42-year-old G2P1001 presented for a routine growth ultrasound at 36 wk 5 d. A: Prenatal ultrasonography shows vein of Galen aneurysm and color flow examination reveals a turbulence flow in the lesion and in the connected strait sinus; B: Prenatal T1-weighted magnetic resonance images show the markedly enlarged median procencephalic vein of Markowsky, characteristic of vein of Galen aneurysmal malformation (arrow); C: Persistence of the falcine draining sinus (arrow).",yes
PMC6175466,Figure_6,oa_package/1a/bb/PMC6175466.tar.gz,['Suppression of NPC tumor growth by inhibitors of lipogenesis.'],"Figure 6 Suppression of NPC tumor growth by inhibitors of lipogenesis. Mice bearing C6661 xenografts were treated with PBS (control), luteolin (20mg/kg) or fatostatin (15mg/kg) every 23days for 3weeks. (A) Tumor size was measured every 23days for 19days. (B and C) At the endpoint, mice were killed, and tumors were (B) weighed and (C) photographed. (D, left panel) The harvested xenografts were embedded for hematoxylin and eosin (H&E) staining and immunohistochemical staining analysis. (D, right panel) The percentage of necrosis, and positive staining for FASN, Ki67, and CC3, were measured, and are shown as dotplots. Lines and bars are mean and standard error of the mean of each experimental group. * <0.05, ** <0.01, *** <0.001, **** <0.0001.",yes
PMC6178129,Figure_1,oa_package/f6/de/PMC6178129.tar.gz,"['To generate HKi- or HKc-specific antibodies, which we expected to be conformational, we immunized hamsters with both purified human HKi and HKc (kallikrein-cleaved human HK; complete cleavage of HK was confirmed by Western blot) (', 'We screened hybridomas generated from immunized animals using direct ELISA with a fluorescent substrate, with plates coated with purified human HKi or HKc proteins (', '', 'Antibody 3E8 bound to both HKi/c (', '0), whereas antibody 2B7 showed specificity to HKi (', '3), and antibody 4B12 specifically bound to HKc (', 'HKi and HKc proteins used in the assay (input, 125 ng each/lane) were shown in ']","Fig.1 Identification of monoclonal antibody specific for HKi/c (3E8), HKi (2B7), and HKc (4B12). (A) Western blot using commercially available anti-HK antibody (Abcam) under reducing conditions shows purified human HKi and HKc used for animal immunization and antibody screening. HKi is observed at 120kDa and the HKc light chain fragment at 45kDa. Representative Western blot is from three independent experiments. The direct ELISA with a fluorescent substrate using purified (B) 3E8 (HKi/c), (C) 2B7 (HKi), and (D) 4B12 (HKc) antibody (0.0043g/mL). HKi and HKc (50ng/well) were coated on the plate. The antibodies 3E8, 2B7, and 4B12 showed specificity for HKi/c, HKi, and HKc, respectively. Values are presented as meanSD of experiments performed in triplicate. Abbreviations: HKi, intact high-molecular-weight kininogen; HKc, cleaved high-molecular-weight kininogen; SD, standard deviation.",yes
PMC9765459,Figure_3,oa_package/71/61/PMC9765459.tar.gz,"['Both vaccines had elicited cross-binding plasma IgG responses to 11 different SARS-CoV-2 variant S proteins () that peaked at week 8 (', '529), were maintained in RMs of both vaccine groups to week 52 (B and C).', '.']","Fig. 3. SARS-CoV-2 vaccination resulted in increased breadth of the plasma antibody response. ( )Each line represents the longitudinal measurements of the plasma IgG breadth scores of individual RMs in the Protein+3 M-052-SE (left graph) or mRNA-LNP (right graph) group. Arrows indicate time of vaccination. ( ) The heatmap shows the median antibody binding as the log median fluorescence intensity (MFI) to 11 different S proteins over time in RMs vaccinated with the Protein or mRNA vaccine. Each row represents the antibody binding log MFI against one specific S protein (see legend for color code) and each column represents a specific week post-immunization. Antibody binding to the SARS-CoV-2 nucleoprotein (N protein) was negative at all time points. ( ) Shown is a comparison of plasma antibody binding to the S proteins of the B.1.617.2 (Delta) and B.1.1.529 (Omicron) variants at peak response (wk 8) and at the time of challenge (wk 52) in RMs of the Protein and mRNA vaccine group. Horizontal lines represent group medians, each symbol represents an individual animal (table S1) with n=8 RMs per group. Differences in antibody binding to the two distinct S proteins within each group at the same time point were determined by Mann-Whitney test with *p<0.05.",yes
PMC8380417,Figure_4,oa_package/cd/41/PMC8380417.tar.gz,"[' 4).', 'Wall motion alterations associated to irregular wall thinning and marked biventricular fibrofatty replacement on LGE images (b, d), with extensive involvement of the LV in left-dominant arrhythmogenic cardiomyopathy (d)Diagnosis is based on structural, functional, electrophysiological and histological abnormalities, included in the 2010 International Task Force (ITF) criteria [35].', ' 4) and sarcoidosis.']","Fig. 4 Biventricular and left dominant arrhythmogenic cardiomyopathy. Cine and LGE images in genetic confirmed arrhythmogenic cardiomyopathy with biventricular ( , ) and left-dominant involvement ( , ). Cine images show enlarged ventricles, with wall motion alteration of free RV wall (hypokinesia in and marked dyskinesia in ) and of lateral LV wall (arrows in a and c), with biventricular reduced EF (50% in both cases). Wall motion alterations associated to irregular wall thinning and marked biventricular fibrofatty replacement on LGE images ( , ), with extensive involvement of the LV in left-dominant arrhythmogenic cardiomyopathy ( )",yes
PMC6172591,Figure_2,oa_package/75/6d/PMC6172591.tar.gz,"['Lateral chest X-rays of patients showing the normal thoracic spine (A), thoracic spine with pre-stage diffuse idiopathic skeletal hyperostosis (DISH) (B), and thoracic spine with DISH (C).']","Fig. 2 Lateral chest X-rays of patients showing the normal thoracic spine (A), thoracic spine with pre-stage diffuse idiopathic skeletal hyperostosis (DISH) (B), and thoracic spine with DISH (C).",yes
PMC4995087,Figure_9,oa_package/1d/d5/PMC4995087.tar.gz,"[') and that markedly rose up with time over the 9 d of assay ( A).', ', whereas mice infected with the same dose of TgIST showed a 60% survival ( B).', 'We next quantified parasites recovered from the peritoneal cavity after injection of higher dose of tachyzoites (105) and confirmed the rapid control of the TgIST population when compared with WT parasites using parasite DNA quantification by real-time PCR ( C) or GFP-based detection of the tachyzoites (', '.', '0.', 'To investigate whether TgIST contributes to the early innate events that determine the priming of adaptive immunity, we measured local (peritoneal fluid, C) and systemic (plasma, not depicted) cytokine profiles over time, after infection.', ', we observed that mice infected with TgIST-deficient parasites produced significantly less of the late proinflammatory IFN- , IL-6, IL-1 , and IL-18 cytokines and antiinflammatory IL-10 cytokine in their peritoneal cavities than mice infected with the parental strain ( C).', 'gondii remained unchanged from days 5 to 9 ( C).', 'Therefore, homing of the CD11b+ Gr1int Ly6G cells is likely to play a pivotal role in the control of TgIST-deficient parasite expansion (Figs.', 'Accordingly, we observed a rapid control of the TgIST-deficient parasites in mice () that also concurred with a highly efficient i.', 'gondii (, A and B).']","Figure 9. (A) Luminescent parasites were imaged with an IVIS imaging system from day 0 to 9 after the i.p. inoculation to BALB/c of 5.10 tachyzoites per condition. Graph on the right depicts mean whole-animal radiance. (B) Virulence of strain was compared with its parental 76KGFP in BALB/c mice. Mice ( = 8) were inoculated with 5 10 tachyzoites by i.p. injection, and survival was monitored. Significance was tested using Log-rank (Mantel-Cox) test (**, p-value = 0.0161) and Gehan-Breslow-Wilcoxon test (**, p-value = 0.0154). (C) BALB/c mice were given i.p. a dose of 10 76KLUC-WT or 76KLUC tachyzoites. Peritoneal lavage fluids were collected on days 2, 5, 7, and 9 after inoculation. Number of tachyzoites was estimated within the collected samples by parasite DNA PCR and concentrations of IL-1, IL-6, IL-10, IL-12, IL-18, and IFN- were determined by ELISA. Data shown are means SD with = 3 individual mice per parasite genotype at each time point.",yes
PMC9424300,Figure_4,oa_package/fe/fb/PMC9424300.tar.gz,"['4A C).', '4D, E).', 'A1 deletion increases pathological neovascularization and decreases vascular repair at P17.', 'Analysis of vascular tortuosity following the OIR exposure using fluorescein angiography showed persistent vascular tortuosity on P50 (', '4F, G, ']","Fig. 4 A1 deletion increases pathological neovascularization and decreases vascular repair at P17. WT and A1 KO littermates were subjected to OIR at P7 and prepared for analysis on P17. Avascular area (AVA, yellow outline) and pathological RNV (tufts, highlighted in white) were significantly increased in A1 KO retinas. Scale bar=100m. , Higher magnification images show decreased vessel sprouts at the AVA border zone of the P17 lectin-labeled A1 KO retina flatmounts. Scale bar=20m. , Fluorescein angiography images show persistent vessel tortuosity in the WT OIR retinas through P50, which was increased in the A1 KO retinas. Data are presented as meanSD in all the figures.",yes
PMC11384802,Figure_5,oa_package/b9/ba/PMC11384802.tar.gz,"[' -Syn mice are resistant to high-fat diet-induced impaired glucose tolerance/insulin resistanceIn line with the body weight data, -Syn mice on HFD exhibited lower fasting blood glucose levels and a more rapid clearance of blood glucose during the intraperitoneal glucose tolerance test (IPGTT) than control-HFD mice ().', 'Although the 4-month male -Syn-HFD mice had better glucose tolerance than age-matched control-HFD mice, their glucose levels did not return to baseline at 120 minutes (b); however, the glucose levels of the 4-month -Syn-HFD female mice did return nearly to baseline by this time (', 'Unlike the 4-month male -Syn-HFD mice, the glucose tolerance of 12-month male -Syn-HFD mice (c) was indistinguishable from that of control mice on a regular diet.', 'Note that in the 1-month males, we measured only fasting glucose levels, which indeed were lower in -Syn-HFD mice than in control-HFD mice (a).', ': -Syn mice on high-fat diet exhibit faster clearance of blood glucose.']","Fig. 5: -Syn mice on high-fat diet exhibit faster clearance of blood glucose. Fasting blood glucose levels in 1-month male group ( ) and IPGTT in 4-month male ( ), 12-month male ( ) and 4-month female ( ) groups after 1416 weeks of high-fat diet treatment. The number of mice for each group is shown in parentheses. Statistical analysis was performed separately for the high-fat diet ( = 0.0085) and regular diet sub-groups ( = 0.2181) in the 1-month male group using unpaired t-tests. For the 4-month male and female groups, analysis was performed using the mixed effects model ( < 0.0001) due to unequal sample sizes. Three-way repeated measures ANOVA was used for the 12-month male group due to equal sample sizes ( < 0.0001).",yes
PMC5342686,Figure_4,oa_package/05/6b/PMC5342686.tar.gz,"['BEA reduced the numbers of eosinophils, lymphocytes, macrophages, neutrophils and total cells in mice BALFThe numbers of eosinophils A.']","Figure 4 BEA reduced the numbers of eosinophils, lymphocytes, macrophages, neutrophils and total cells in mice BALF The numbers of eosinophils , lymphocytes , macrophages , neutrophils and total cells in mice BALF. Data are presented as meanSD of 8 mice each group. Statistical analysis between highlighted groups was determined by student's -test. Significance level was labeled as: ns; <0.05; * <0.05; ** <0.01; *** <0.001.",yes
PMC4663208,Figure_4,oa_package/33/18/PMC4663208.tar.gz,"['Before IG-HDR was performed, the patient had prior chemoradiation and an abdominoperineal resection procedure, followed by adjuvant chemotherapy; at the time of IG-HDR, he had a bulky, painful local recurrence (A).', 'Subsequent to IG-HDR, he had a significant clinical response, with improvement in pain and significant reduction in tumor burden (B).', 'Unfortunately, he subsequently developed a bulky, painful local recurrence after his initial response, and it was at this point that the fistulization manifested (C).', 'The clinical course for patient 1 is shown pictorially.']","Fig. 4 The clinical course for patient 1 is shown pictorially. On the left is the gross disease present at the time of IG-HDR treatment. Tumor burden 4.5 months after treatment is shown in the middle. Unfortunately, the patient had subsequent progression, resulting in bulky local recurrence, shown on the right at one year following IG-HDR",yes
PMC6624000,Figure_1,oa_package/3d/c8/PMC6624000.tar.gz,"['Left (A): CT of the chest, abdomen, pelvis without contrast read as: No malignancy within the chest, abdomen, or pelvis, nonspecific splenomegaly, and calcified pleural plaques.']","Figure 1 Left (A): CT of the chest, abdomen, pelvis without contrast read as: No malignancy within the chest, abdomen, or pelvis, nonspecific splenomegaly, and calcified pleural plaques. Right (B): Nuclear medicine PET tumor scan read as: Splenomegaly with markedly hypermetabolic large foci of hypermetabolic activity, concerning for lymphoma (arrow). No additional or extra-nodal disease is seen in the chest, abdomen, and pelvis. CT: computed tomography; PET:positron emission tomography",yes
PMC3636081,Figure_4,oa_package/9a/51/PMC3636081.tar.gz,['(a): Posterior spiracles of third stage-larva of Lucilia spp.'],Figure 4 : Cephalopharyngeal skeleton (black arrow) attached to mouth-hooks (blue arrows) that lacked accessory oral sclerite (scale: 200m).,yes
PMC3449008,Figure_2,oa_package/db/42/PMC3449008.tar.gz,"['From P11-P18, however, Cyclin D2 was consistently more abundant in cerebella from Bax-deficient animals, indicating Bax deletion delayed the down-regulation of CGNP proliferation seen in the wild-type littermates (a).', 'Western blot analysis demonstrated that Shh abundance was not increased by Bax deletion and thus could not account for increased CGNP proliferation (a).', 'Bax+/+ and Bax / CGNPs demonstrated equivalent baseline expression of Cyclin D2 and equivalent increase in Cyclin D2 in response to Shh (b).', 'While dexamethasone induced substantial caspase activation in cerebellum of wild-type mice, Bax deletion markedly reduced cell death in dexamethasone-injected mice, reducing cC3 without affecting total Caspase 3 abundance (c).', 'Western blot on Bax+/ and Bax / littermates demonstrated no change in Bak expression caused by Bax deletion (d), indicating that loss of Bax alone was sufficient to prevent dexamethasone-induced apoptosis.', 'Importantly, Bax deletion caused a detectable increase in expression of Bcl-2 and Mcl-1 (d), which may have contributed to decreasing the apoptotic response by interfering with Bak-mediated compensation.', 'Bax deficient CGNPs have normal response to Shh but altered response to pro-apoptotic stimulusA) A representative Western blot demonstrates temporal expression patterns of indicated proteins in lysates of whole cerebella harvested from Bax+/+ and Bax / littermates at the ages indicated, with -Actin used as a loading control.']","Figure 2 Bax deficient CGNPs have normal response to Shh but altered response to pro-apoptotic stimulus A representative Western blot demonstrates temporal expression patterns of indicated proteins in lysates of whole cerebella harvested from Bax and Bax littermates at the ages indicated, with -Actin used as a loading control. While Cyclin D2 elevation waned more slowly in Bax mice, Shh abundance remained constant over time and did not vary with genotype. Differential expression of Cyclin D2 was noted in at least 3 paired Bax and Bax littermates at each time point from P11-P17. Comparison of proliferation of CGNPs isolated from Bax and Bax littermates and cultured in the presence or absence of exogenous Shh, measured by Western blot for Cyclin D2, demonstrated that proliferative response to Shh was not affected by Bax deletion. 3 replicate wells for each condition demonstrated equivalent findings. Representative Western blot comparing apoptosis induced by dexamethasone in Bax and Bax littermates, detected by cC3 in whole cerebellar lysates 24 hours after IP injection of dexamethasone or saline. Examination of Bcl-2 family proteins over CGNP development, in Bax and Bax mice. A SmoA1-induced medulloblastoma is included for comparison.",yes
PMC7517961,Figure_6,oa_package/02/b6/PMC7517961.tar.gz,"['Activity of calpain and caspase-3 was measured via western blot analysis of their specific II-spectrin breakdown products (A and 6B).', 'Markers of proteolytic activity.', 'Specifically, the ratio of LC3II to LC3I in the heart of TB-SALINE mice was significantly elevated compared to non-tumor-bearing animals (C); whereas in the diaphragm there were no differences in the expression in this marker of autophagy (D).', 'Conversely, mRNA expression of the Bcl-2 protein family member BNIP3 and the E3 ubiquitin ligase MuRF1 were elevated in TB-SALINE mice compared to controls in the diaphragm (E).', 'In the heart, no differences existed among groups in the expression of MuRF1, and BNIP3 gene expression was reduced in the CON-SS-31 mice compared to CON-SALINE (F).']",Figure 6 Markers of proteolytic activity. Western blot analysis of the calpain (145 kDa) and caspase-3 (120 kDa)-specific spectrin breakdown product in ( ) heart and ( ) diaphragm for control (CON) and tumor-bearing (TB) mice treated with saline (SALINE) or SS-31. Western blot analysis of LC3II/LC3I in ( ) heart and ( ) diaphragm for control (CON) and tumor-bearing (TB) mice treated with saline (SALINE) or SS-31. Gene expression of MuRF1 and BNIP3 in ( ) heart and ( ) diaphragm for control (CON) and tumor-bearing (TB) mice treated with saline (SALINE) or SS-31. Values are represented as means SEM. Representative western blot images are shown below the graphs. significantly different versus all groups ( < 0.05); significantly different versus CON-SALINE and CON-SS-31 ( < 0.05).,yes
PMC9347127,Figure_5,oa_package/e4/eb/PMC9347127.tar.gz,"['5A C and Additional file 1: ', 'Meanwhile, TA also recovered the cell viability of mouse primary hippocampal neurons as evidenced by the decreased ratio of PI/Hochest of cells (D).', '5A C and Additional file 1: ', 'TA ameliorates neuronal damage induced by the A (1 42)-induced activation of the NLRP3 inflammasome in microglial cells.', '(One-way ANOVA with Tukey-corrected post hoc t-test for multiple comparisons was applied for comparison between groups)TA induces autophagy and exhibits neuroprotective effects in C.']","Fig. 5 TA ameliorates neuronal damage induced by the A(142)-induced activation of the NLRP3 inflammasome in microglial cells. BV-2 cells were treated with 5 M of A(142) for 24 h, followed by the treatment of TA (10 M) in the presence or absence of inhibitors, including LY, CC, and SCH for an additional 24 h. The conditioned mediums were then transferred into PC-12 cells and incubated for 24 h. The cell viability of PC12 cells was then detected by ( ) MTT assay, ( ) Hoechst 33342/PI staining, and ( ) flow cytometry methods. Bar charts indicate the cell viability, the PI/Hoechst ratio, and the apoptosis rate of PC-12 cells. The representative fluorescence images were captured by a fluorescence microscope. Magnification, 20; scale bar: 100 m. Mouse primary microglial cells were treated with 5 M of A(142) for 24 h, followed by the treatment of TA at the indicated concentrations for 24 h. The conditioned mediums were then transferred into mouse primary hippocampal neurons and incubated for 24 h. The cell viability of mouse primary hippocampal neurons was then detected by Hoechst 33342/PI staining method. The representative fluorescence images were captured by a fluorescence microscope. Magnification, 20; scale bar: 100 m. The bar chart indicates the PI/Hoechst ratio of mouse primary hippocampal neurons. Error bars, S.D. * 0.05; ** 0.01; *** 0.001. = 3. (One-way ANOVA with Tukey-corrected post hoc -test for multiple comparisons was applied for comparison between groups)",yes
PMC2682408,Figure_7,oa_package/f9/73/PMC2682408.tar.gz,"[' 7) and palpated while a valgus stress is applied.', ' 7Muscle split approach and ulnohumeral gapping demonstrated after MCL incised\nA drill hole is made at the site of the anatomic origin of the anterior bundle of MCL on the medial epicondyle that does not penetrate the posterior cortex.']",Fig.7 Muscle split approach and ulnohumeral gapping demonstrated after MCL incised,yes
PMC6532138,Figure_1,oa_package/0b/b6/PMC6532138.tar.gz,[':Erect AXR showing Air-Fluid levels and distended large bowel loops.'],Figure 1: Erect AXR showing Air-Fluid levels and distended large bowel loops.,yes
PMC10560129,Figure_1,oa_package/9b/d7/PMC10560129.tar.gz,"['Stomach (high magnification): one noncaseating granuloma made up of a mix of lymphocytes, plasma cells, and epithelioid histiocytes involving the stomach lamina propriaDiscussionInvolvement of the GI tract in sarcoidosis is extremely rare.']","Figure 1 Stomach (high magnification): one noncaseating granuloma made up of a mix of lymphocytes, plasma cells, and epithelioid histiocytes involving the stomach lamina propria",yes
PMC9283810,Figure_2,oa_package/dd/47/PMC9283810.tar.gz,[],Figure 2 Ulcration circonfrentielle du tiers postrieur de la langue Circumferential ulceration of the posterior third of the tongue,yes
PMC7243621,Figure_5,oa_package/2c/0f/PMC7243621.tar.gz,['The right common femoral vein was accessed and a 2-mm Snare delivery catheter was advanced over a Stork wire.'],"Figure 5 The right common femoral vein was accessed and a 2-mm Snare delivery catheter was advanced over a Stork wire. Under fluoroscopic observation, the looped shunt catheter was snared and withdrawn to the right common femoral vein (arrow), where it was ultimately removed surgically through venotomy.",yes
PMC6861570,Figure_2,oa_package/67/4e/PMC6861570.tar.gz,"['Computed tomography (CT) findings (Fig 2) were notable for aortic wall thickening with surrounding lymphadenopathy concerning for infection.', 'Fig 2Computed tomography (CT) scan at the time of presentation with an increase in the size of the aneurysm sac and significant periaortic thickening and lymphadenopathy.']",Fig2 Computed tomography (CT) scan at the time of presentation with an increase in the size of the aneurysm sac and significant periaortic thickening and lymphadenopathy.,yes
PMC3108502,Figure_1,oa_package/7c/38/PMC3108502.tar.gz,"['In the pharynx and larynx, mucositis and submucosal edema result in prominent mucosal contrast enhancement with thickening of the epiglottis and aryepiglottic folds ().', 'These sarcomas may present as an enhancing soft tissue, defined mass and/or bone destruction (1).', 'Congenital cystic masses of the neck: radiologic-pathologic correlationRadiographics1999191121146992539632SinghSRosenthalDIGinsbergLEEnlargement and transformation of thyroglossal duct cysts in response to radiotherapy: imaging findingsAmerican Journal of Neuroradiology20093048008021913141533LeeAWMLawSCKNgSHRetrospective analysis of nasopharyngeal carcinoma treated during 1976 1985: late complications following megavoltage irradiationBritish Journal of Radiology199265778918928142266734ChongVEHFanYFRadiation-induced temporal lobe necrosisAmerican Journal of Neuroradiology1997184784785912705135GlassJPHwangTLeavensMELibshitzHICerebral radiation necrosis following treatment of extracranial malignanciesCancer198454919661972647843136HardinCWHarnsbergerHROsbornAGInfection and tumor of the masticator space: CT evaluationRadiology19851572413417404844937PatelSGSeeACHWilliamsonPAArcherDJRhys EvansPHRadiation induced sarcoma of the head and neckHead and Neck19992143463541037675538BradyMSGaynorJJBrennanMFRadiation-associated sarcoma of bone and soft tissueArchives of Surgery199212712137913851365680Postradiation changes of the oropharynx: Axial postcontrast CT demonstrates mucositis of the oropharynx characterized by enhancement (large arrow), and edema/swelling, or the epiglottis (small arrow).', '010""/>1Radiation-associated osteosarcoma of the mandible: (a) Axial contrast-enhanced CT of the mandible (bone window) shows an osteoid matrix (arrow) within the tumor.']","Figure 1 Postradiation changes of the oropharynx: Axial postcontrast CT demonstrates mucositis of the oropharynx characterized by enhancement (large arrow), and edema/swelling, or the epiglottis (small arrow).",yes
PMC7104903,Figure_1,oa_package/ba/c4/PMC7104903.tar.gz,"['IAV mice that were treated with MitoTEMPO lost significantly less of their total body weight in comparison with the virus-infected vehicle-treated group (A).', 'Moreover, mice treated with MitoTEMPO showed a significant increase in survival rate (B).', 'Importantly, MitoTEMPO-treated naive mice did not lose any body weight over the course of the experiment (A).', '7727_figure1""/>To evaluate airway inflammation, the total number of live cells in the bronchoalveolar lavage (BAL) was counted.', 'Intranasal challenge with Hkx-31 IAV caused an increase in airway inflammation at day 3 and 5 postinfection (C, D, respectively).', 'Infection with IAV caused a rapid infiltration of circulating neutrophils, macrophages, lymphocytes, eosinophils, and natural killer cells (C, D).', 'MitoTEMPO treatment caused a marked 40% 60% reduction in neutrophil cell counts and a 50% 60% reduction in eosinophil cell counts at both of these time points (C, D).', 'At day 3, the number of macrophages and natural killer cells was also unaltered by MitoTEMPO treatment, but there was a significant reduction in BAL fluid (BALF) macrophages at day 5 (D).', 'Supplementary MaterialSupplemental dataG, right).', 'The intact and mutated reporter constructs were also transfected into PM cultures normally expressing Runx1 (F).']",10.1371/journal.pgen.1005457.g002,yes
PMC4236374,Figure_2,oa_package/ef/47/PMC4236374.tar.gz,['.'],"Fig. 2. Intraoperative photography demonstrates the tumor reflected en bloc exposing the brachial artery (forceps), partially resected biceps muscle, and basilic vein (loop).",yes
PMC3720854,Figure_4,oa_package/8e/fe/PMC3720854.tar.gz,"['03) of proteinuria, respectively (A).', 'g004hKLK1-MSCs transfer ameliorated spontaneous lupus nephritis in B6.', '80 mg/dL, respectively, B).', 'As shown in C F, hKLK1-MSCs treated mice exhibited mild to moderate mesangial hypercellularity with patent or narrowed capillary lumina and slightly thickened capillary walls (', '8 (C), whereas the mice in the PBS control group showed severe renal injury characterized by diffuse global intracapillary hypercellularity with narrowed or obliterated capillary lumina and thickened glomerular capillary walls (', '5 (C).', '8 (E and C).']",10.1371/journal.pone.0067790.g004,yes
PMC8642678,Figure_4,oa_package/88/8b/PMC8642678.tar.gz,[],"FIGURE 4 Isolated progenitor cells differentiate to mesenchymal lineages in vitro. Cells from pericard (A), pleura (B), adipose tissue (C) and trachea (D) all showed the capacity to adequately differentiate to chondrogenic (CH), osteogenic (OS) and adipogenic (AD) lineages. Differentiation did not occur in control groups. Chondrogenesis is indicated by glycosaminoglycan staining using Alcian blue. Osteogenesis was confirmed using AlizarinRed staining for calcium deposits. Accumulation of lipid vacuoles that stain with Oil Red O indicated adequate adipogenesis. Staining intensities were comparable. Magnification 400. N=3.",yes
PMC5166487,Figure_8,oa_package/76/98/PMC5166487.tar.gz,"[' Representative micrographs of immunohistochemical technique against active caspase-3 antibody in spleen, thymus and lung from BTV-4-infected and Mock animals.']","Figure 8 The number of active caspase-3 cells was moderately increased in spleen, thymus, and lung from BTV-infected animals, concurring with the apoptotic debris.",yes
PMC8614797,Figure_3,oa_package/c1/25/PMC8614797.tar.gz,"['Because the APP/PS1 mice develop A deposits by 6 mo of age, analysis of A deposits using 4G8 staining of brain sections from 10- and 14-mo-old APP/PS1 mice showed severe A pathology in both cortex and hippocampus (, s S3 and S4).', 'Quantitative analysis of the S1BF region of the cortex and the hippocampus for the fraction of total area covered by A -deposits confirmed that -GSH-treatment of aged APP/PS1 mice lead to a significant reduction in A plaques, compared to their saline-treated APP/PS1 littermates (a c and S3).', 'With -GSH treatment, insoluble A 42 levels were decreased in both 10-mo and 14-mo-old APP/PS1 mice (d,e)Glycation-induced oxidative stress is known to increase neuroinflammation and promotes accelerated A aggregation and toxicity [5].', ' -GSH treatment reduces A burden in symptomatic 10-mo-old APP/PS1 mice.']","Figure 3 -GSH treatment reduces A burden in symptomatic 10-mo-old APP/PS1 mice. ( ) Representative images of A plaques visualized using 4G8 antibody in S1BF cortex and dentate gyrus of 14-mo NTG and APP/PS1 mice. Scale bar, 100 m; ( ) levels of A plaques are significantly reduced in S1BF and hippocampus in -GSH-treated 10-mo APP/PS1 mice ( = 5 APP/PS1 + Saline; = 4 APP/PS1 + -GSH); ( ) levels of A plaques are significantly reduced in S1BF and hippocampus in -GSH-treated 14-mo APP/PS1 mice ( = 3 APP/PS1 + Saline; = 4 APP/PS1 + -GSH); ( , ) quantitation of the effect of -GSH treatment on amyloid load in APP/PS1 mice using ELISA assay. Levels of insoluble A levels in the brain homogenate of mice treated with -GSH were significantly reduced in both 10-mo ( ) and 14-mo ( ) cohorts. For comparisons between saline and -GSH-treated APP/PS1 groups ( , ), an unpaired Students -test was performed for statistical analysis. * < 0.05, ** < 0.01, *** < 0.005.",yes
PMC3022224,Figure_4,oa_package/fa/0c/PMC3022224.tar.gz,"['The degree of BMD reduction is dictated by the extent of leukocyte infiltration as osteoclast recruitment along bony surfaces is induced by numerous proinflammatory cytokines [155]; accordingly, bone dissolution is more severe in AIA (), where leukocyte influx is more severe than in CIA or SCW.', '003""/>Bone mineral density (BMD) of the tibiotarsal region (hock (or ankle )) by dual X-ray absorptiometry (DXA) is a rapid means of evaluating bone integrity in arthritic rodent hind paws.']","Figure 4 Bone mineral density (BMD) of the tibiotarsal region (hock (or ankle)) by dual X-ray absorptiometry (DXA) is a rapid means of evaluating bone integrity in arthritic rodent hind paws. Relative to a nonarthritic control (a), BMD is greatly reduced in multiple bones (bordered by the yellow rectangle) of a young adult, male Lewis rat with the mycobacterial variant of adjuvant-induced arthritis (AIA-Myc). These panels are reproduced from [ ] with the permission of the American College of Rheumatology.",yes
PMC7670449,Figure_7,oa_package/bd/80/PMC7670449.tar.gz,"[' 7a).', ' 7b).', ' 7c).', 'Changes in the expression of microbial sensing genes in WT and fat-1 mice exposed to EtOH.', 'Impact of EtOH on the intestinal genes involved in the adenosine signaling pathway in WT and fat-1 miceAdenosine signaling is recognized as an important endogenous anti-inflammatory pathway in various diseases, including intestinal injury and inflammation21,22.']","Figure 7 Changes in the expression of microbial sensing genes in WT and mice exposed to EtOH. ( ) Relative gene expression of genes in ileal mucosa from WT PF mice. Data are presented as meanSEM for FPKM. ( ) Gene expression of genes in ileal mucosa from all treatment groups. Data are expressed as fold changes vs WT PF set as 1, * p<0.05. ( ) Heatmap analysis of ileal mucosa genes across multiple comparisons. Data are expressed as fold changes for indicated groups. Significance for the PF vs WT PF comparison is denoted by ( ), for the WT EtOH vs WT PF ( ), for EtOH vs PF ( ), and for EtOH vs WT EtOH ( ). n=35 mice per group.",yes
PMC3429492,Figure_4,oa_package/0d/e5/PMC3429492.tar.gz,"['Baseline lavages were performed before each study cycle revealing almost only macrophages (C, 5B, 6A).', 'g004Changes in absolute cell numbers in bronchoalveolar lavage (BAL) fluid after LPS challenge.', 'LPS induced a significant influx of inflammatory cells with increased total cell counts into the challenged lung lobe (A, p = 0.', 'Neutrophils showed the most pronounced increase after LPS challenge (B, 5A, 6B).', 'In sham-treated animals (positive control) absolute macrophage (C, p = 0.', '26) and lymphocyte counts (D, p = 0.', 'Compared with the positive control sham, pre-treatment with roflumilast resulted in a statistically significant decrease in absolute and relative neutrophil cell numbers in BAL (B, p = 0.', '04; C, p = 0.', 'Roflumilast pre-treatment had no influence on lymphocyte counts (D, 5C).', 'Pre-treatment with the treatment control dexamethasone revealed also a reduction in neutrophil numbers (B, ', 'Additionally, there was no effect on macrophages and lymphocytes after dexamethasone pre-treatment (C, p = 0.', '23; D, p = 0.']",10.1371/journal.pone.0043709.g004,yes
PMC4655344,Figure_1,oa_package/3b/f4/PMC4655344.tar.gz,"['\nSmall vessel disease pathways disrupts subcortical pathways that are important for emotion regulation.', '\nSmall vessel disease is a stroke subtype characterized by pathology of the small perforating arteries, which supply the sub-cortical structures of the brain.', 'For the structural equation modelling analysis, key variables and covariates were included in an initial hypothesized model () and then pathways were dropped in a systematic fashion to create alternate models for comparison, revealing the most parsimonious model (Supplemental Differential counts of cell populations in the BAL fluids were also assessed to determine a phenotypic difference in cell recruitment to the lungs between the mouse strains.']","Figure 4. Differences in plasma cytokine concentrations between the two strains were detected early following infection. Significantly more (a and b) CXCL1 (KC) and(c and d) G-CSF was detected in plasma from mice expressing hTLR4/MD-2 compared to wild type mice early after infection (16 or 24 h) with 1000 or 10,000 GE , respectively. (e) By 6 d post infection, in contrast, the detection of G-CSF was lower, and levels were not significantly different between the two strains. All graphs represent the average of at least four mice per group with standard deviation shown. ** <0.01, *** <0.001, and **** <0.0001 as measured in pairwise comparisons of two groups using Students -test.",yes
PMC4836167,Figure_2,oa_package/fd/d7/PMC4836167.tar.gz,['Principal cephalic pain pathways and meningeal mast cell activation in migraine.'],"Figure 2 . Left: the initiation of migraine headache follows activation of nociceptors innervating meningeal blood vessels. Pain information flows from these nociceptors the trigeminal nerves (TNs) to the trigeminal ganglion (TG), which receives input from the meninges mainly the ophthalmic branch of the trigeminal nerve (V1), and to a lesser extent from the maxillary (V2) and mandibular (V3) divisions. Pain information is then transmitted to the trigeminocervical complex (TCC), which comprises the C1 and C2 dorsal horns of the cervical spinal cord and the caudal division of the spinal trigeminal complex. The occipital cervical nerves (OcNs) sense posterior head and neck pain (common in migraineurs). These pain signals traverse the dorsal root ganglion (DRG) where they also terminate in the TCC. Right: an enlarged view highlighting mast cell activation within the meninges and brain. Activation of meningeal nociceptors leads to the release of vasoactive proinflammatory peptides, such as calcitonin gene-related peptide and substance P from terminal nerve endings (colored circles near terminals), resulting in meningeal BV vasodilatation, and local activation of dural mast cells (MC). Mast cell estrogen receptors ER and ER, and progesterone receptors A (PR-A) and B (PR-B) are located at the plasma membrane or in the nucleus, and mediate mast cell responsiveness to these sex steroids. Following mast cell degranulation by either meningeal nociceptor activation, [or experimental nitroglycerine (NTG) injections], mast cells secrete vasoactive factors (VAF) and cytokines, such as nitric oxide, TNF, vasoactive intestinal peptide, and histamine (depicted by colored circles) in meninges and brain. Mast cells can also react to neuronal stimuli, including substance P, CGRP, corticotropin-releasing hormone, and histamine. Mast cell degranulation can also lead to disruption of the brainbrain barrier (BBB), which is depicted by astrocytic end feet (blue) and pericytes (green) that directly appose brain capillaries.",yes
PMC4435912,Figure_1,oa_package/64/ab/PMC4435912.tar.gz,"[' 1 shows representative monochromatic DAPI-stained images of TMA cores with low (1.', 'Representative DAPI-stained images of individual tissue microarray (TMA) cores used as substrates for nuclear fractal dimension (nFD) analysis.', 'Five-year disease-specific survival (DSS) in OSCC patients stratified by nuclear fractal dimension (nFD).']","Figure 1 Representative DAPI-stained images of individual tissue microarray (TMA) cores used as substrates for nuclear fractal dimension (nFD) analysis. Image of an entire TMA core with low nFD, intermediate nFD and high nFD.",yes
PMC3179601,Figure_2,oa_package/b3/e5/PMC3179601.tar.gz,[],Figure 2 Reduced airway pathology and inflammation following adoptive transfer of CD4 cells from helminth-infected donors. Experiments were performed as in with transfer of purified CD4 and CD4 MLNC from 28 day -infected mice. (A) On day 32 lung sections were stained with hematoxylin and eosin to measure cellular inflammation within the broncho-vascular bundles following airway challenge. D:D denotes mice receiving Der p1 immunizations and challenge. Four individual mice are shown for each group. (B) Experiments were performed as in with transfer of CD4 cells from nave or 28 day -infected mice. Airway inflammation was measured by total cell and eosinophil numbers in BAL. Statistical analysis by the MannWhitney test: <0.02.,yes
PMC2627904,Figure_1,oa_package/3d/cd/PMC2627904.tar.gz,"['Malarial retinopathy consists of one or more of the following ocular fundus findings: hemorrhages, whitening of the retina, orange to white discoloration of the retinal vessels and papilledema ().', 'g001Fundus photograph displaying malarial retinopathy consisting of multiple white centered hemorrhages, macular whitening (arrowheads) and orange discoloration of vessels (arrow).']",10.1371/journal.pone.0004317.g001,yes
PMC10906230,Figure_5,oa_package/f3/a8/PMC10906230.tar.gz,"['Indeed, by 2-color RISH, we observed epithelial IGF1R expression adjacent to IGF1+ stroma (, A, B, and E, and quantified in Supplemental Table 4).', 'Perhaps more surprisingly (given the studied role of CXCL13 in lymphocyte chemoattraction), we also observed epithelial expression of the CXCL13 receptor CXC chemokine receptor 5 (CXCR5) adjacent to CXCL13+ stroma (, C and F).', 'Although expressed at lower levels than IGF1R, CXCR5 expression was clearly visible in comparison with negative control probes (D).', 'Consistent with that function, we observed by immunohistochemistry increased Ki-67+ (proliferating) epithelial cells in BPH hub epithelium adjacent to inner IGF1+CXCL13+ stroma, in comparison with the outer IGF1 CXCL13 stroma (, G and H, and Supplemental ) (P = 0.', 'Perhaps surprisingly then, we observed B and T cell infiltrates within both the inner CXCL13+ stroma and the outer CXCL13 stroma (example shown in I).', 'However, we note that expression of the CXCL13 receptor CXCR5, by quantitative reverse transcription PCR (qRT-PCR), was substantially lower compared with IGF1R in BPH-1 spheroids and BPH organoids (Supplemental ).', 'IGF1 and CXCL13 receptors are expressed in adjacent prostate ductal epithelium.']","Figure 5 IGF1 and CXCL13 receptors are expressed in adjacent prostate ductal epithelium. ( ) H&E stain of Hub-8. Inner stroma (yellow star) and outer stroma (blue star) are indicated; scale bar is 500 m. ( ) Two-color RNA in situ hybridization (RISH) of Hub-8 shown for ( and ) (blue) and (red), ( and ) (blue) and (red), and ( ) negative control probes. Note, 2-color RISH for / and / was conducted on 3 additional BPH hubs ( ). ( ) IHC of Ki-67 identifies proliferating ductal epithelial cells adjacent to stroma. Arrows identify representative Ki-67 cells. ( ) Increased proliferating (Ki-67 ) ductal epithelial cells on the side facing the inner/inductive stroma. Data from 4 hubs (see , quantified as Ki-67 cells per millimeter duct; scale bar is 500 m, and all figure insets are an additional 1.6 magnification. Mean SD shown. * < 0.05; 2-sided paired Students test. ( ) Two-color IHC identifies B cells (CD20, brown) and T cells (CD3, red) within both the inner and outer stroma of hubs. Note, 2-color IHC for CD20/CD3 was conducted on 1 additional BPH hub (Hub-7).",yes
PMC8531757,Figure_3,oa_package/a5/09/PMC8531757.tar.gz,"['5PR-high cells are differentially enriched in immunomodulatory NF-kB signalingThe top significantly upregulated genes in PR-high cells (a) included ubiquitin-specific peptidase 39 (USP39), a pre-catalytic spliceosome member; the Spindle and Kinetochore Associated Complex Subunit (SKA3), a regulator of microtubule attachment to the kinetochores during mitosis; and tropomyosin3 (TPM3), an actin-binding protein that stabilizes cytoskeletal microfilaments.', 'Functional clustering of differential transcriptome in PR-high cells.', '05), functional clustering and pathway analysis was performed using Metascape (b).', 'Intriguingly, PR-high cells were found to differentially express CARD11 and BCL10, members of the CARD-BCL10-MALT1 complex which regulates NF- B signaling (c).', 'To show the variance in population-specific gene expression between bulk analysis of unsorted samples in comparison to LCM-isolated PR-High and PR-Low MCF-7 cells, the normalized expression of the top significantly upregulated genes was assessed in bulk, unsorted MCF-7 cell line samples (a, ']","Fig. 2 ICC-based identification and isolation of phenotypically heterogeneous cells. (a) chemical (i.e. H&E) and Immunocytochemical characterization of MCF-7 cells according ER, PR, and HER2 expression (images taken at 40X magnification using Olympus BX43 microscope). (b and c) Cell count of cells that have high (ER+/PR+) and low expression (ER-/PR-) of (b) ER and (c) PR extrapolated using manual (M) and semi-automatic (A) counting. (d) Selection of PR positive (red arrow) and PR negative cells (yellow arrow) using laser capture microdissection (Images taken at 63X magnification using Leica LMD 6 Laser Capture Microdissection system). (e) Difference in PR gene expression between PR-high and PR-low microdissected cell using qRT-PCR analysis; PR expression was normalized to the geometric mean of the housekeeping genes 18S rRNA, GAPDH, and -actin to calculate the CT. Data in the bar plots presented as meanSEM. Significance is determined using two-tail -test. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)",yes
PMC11412541,Figure_2,oa_package/e6/82/PMC11412541.tar.gz,"['gondii infection resulted in more severe adverse pregnancy outcomes in Trem2-/- mice compared with wildtype mice, as evidenced by the reduced fetal size and weight (A).', 'Additionally, HE staining manifested that the placentas of the infected Trem2-/- mice also suffered pathological damage, accompanied by obvious bleeding and necrosis (B).', 'gondii infection (C and Altered m activates caspase-9 mediated HKM apoptosis.']","Figure 7 Altered activates caspase-9 mediated HKM apoptosis. (a) HKM were infected with and at indicated time p.i. studied using the JC-1 dye. (b) HKM were pre-treated separately with RR, Dant, Xes, CsA, and Z-ATAD-FMK and at 24h p.i. studied using the JC-1 dye by confocal microscopy (40). Red images indicate the JC-1 aggregate, while green images indicate JC-1 monomers. Merged images indicate the co-localization of JC-1 aggregates and monomers. (c) Representative western blot of cyt C release in the cytosol in lysates of HKM pre-treated with CsA at 24h p.i. (d) HKM were pre-treated separately with Z-ATAD-FMK, Z-IETD-FMK, RR, CsA and Z-LEHD-FMK and caspase-9 activity assayed at 24h p.i. in the lysates. Increase was significant in infected samples relative to uninfected HKM which significantly lowered following pre-incubation with inhibitors ( , < 0.001/F = 93.65). (e) Representative western blot of PARP cleavage in lysates of HKM pre-treated with Ac-DEVD-CHO at 24h p.i. -actin served as the loading control. The image represents the best of three replicates. HKM; HKM + B. Vertical bars represent mean SE (n = 6). HKM, uninfected control; HKM + B, HKM infected with ; HKM + Dant + B, HKM pre-treated with dantrolene for 1h before -infection; HKM + Xes + B, HKM pre-treated with xestospongin C for 1h before -infection; HKM + RR + B, HKM pre-treated with RR for 1h before -infection; HKM + Z-ATAD-FMK + B, HKM pre-treated with Z-ATAD-FMK for 1h before -infection; HKM + CsA + B, HKM pre-treated with CsA for 1h before -infection; HKM + Z-IETD-FMK + B, HKM pre-treated with Z-IETD-FMK for 1h before -infection; HKM + Z-LEHD-FMK + B, HKM pre-treated with Z-LEHD-FMK for 1h before -infection; HKM + Ac-DEVD-CHO + B, HKM pre-treated with Ac-DEVD-CHO for 1h before -infection. RR, MUP inhibitor; Xes, IP3R inhibitor; Dant, RyR inhibitor; CsA, MPTP inhibitor; Z-ATAD-FMK, caspase-12 inhibitor; Z-IETD-FMK, caspase-8 inhibitor; Z-LEHD-FMK, caspase-9 inhibitor; Ac-DEVD-CHO, caspase-3 inhibitor.",yes
PMC8279954,Figure_1,oa_package/31/2e/PMC8279954.tar.gz,"['Renal pathology was characterized by moderate-to-severe acute tubular injury in all ( 1\nA).', '5%) ( 1B-F).', 'Marked peritubular capillary congestion was noted in all cases ( 1B and C).', ' 1Acute tubular injury and immune cell infiltration in coronavirus disease 2019 kidneys.']","Figure1 Acute tubular injury and immune cell infiltration in coronavirus disease 2019 kidneys. The renal cortex on light microscopy shows acute tubular injury with lumina ectasia (hematoxylin and eosin [HE] stain, original magnification20). Mild mononuclear interstitial infiltration and peritubular capillary congestion is noted (HE stain, original magnification40). ( and ) Peritubular capillaritis ( ) is a notable feature within congested capillaries . The microvascular inflammation is composed of CD3-positive T cells and CD68-positive macrophages (original magnification40 for E and F).",yes
PMC8506641,Figure_4,oa_package/d2/4a/PMC8506641.tar.gz,"['Progression of metastasis to the right iliac lymph nodes was detected in January 2017 after only four cycles of FOLFOX with bevacizumab ().', 'Distant lymph node metastasis after anastomosis site colectomy and lymphadenectomy followed by four cycles of fluorouracil, leucovorin and oxaliplatin plus bevacizumab (pre-regorafenib).']","Figure 4 Distant lymph node metastasis after anastomosis site colectomy and lymphadenectomy followed by four cycles of fluorouracil, leucovorin and oxaliplatin plus bevacizumab (pre-regorafenib). (A) APCT showing superior mesenteric artery lymph node metastasis with clip marking (arrow). (B) APCT showing right common iliac lymph node metastasis (arrow). (C) Positron emission tomography-CT showing multifocal lymph node metastasis. APCT, abdominopelvic CT.",yes
PMC9385148,Figure_2,oa_package/5e/d1/PMC9385148.tar.gz,"['There was no significant difference in peripheral blood counts, namely, absolute neutrophil count (ANC) or absolute lymphocyte count (ALC), between the two treatment groups (, A and B); however, administration of AMY-101 led to a significant reduction in CRP and ferritin levels on day 7 (P = 0.', '023, respectively), indicating an anti-inflammatory effect of C3 inhibition (, C and D).', '.']","Fig. 2. Hematologic and inflammatory markers. ( ) ANC, ( ) ALC, ( ) CRP, and ( ) ferritin levels on day 1 (baseline) and day 7 after the initiation of treatment. For (A) to (D), a Mann-Whitney test was performed for unpaired analysis across groups, whereas paired analysis within a single group was performed using the Wilcoxon test; means SD. * < 0.05, ** < 0.01, and *** < 0.001.",yes
PMC10839408,Figure_1,oa_package/a0/ce/PMC10839408.tar.gz,"['"" id=""feb413711-fig-0001"">Disease associated characteristics of tauopathies.']","Fig. 1 Diseaseassociated characteristics of tauopathies. (A) Tauopathies can be classified into three groups: 3R+4R tauopathies, including AD, 3R tauopathies, including PiD and 4R tauopathies, including PSP and CBD. (B) Representative electron microscopical images of tau assemblies in AD, PSP, and PiD brains. Inboxes show PHFs and SFs in AD, SFs and twisted filaments in PSP, and SFs with some wide twisted filaments in PiD. (C) The core structures of tau filaments observed by cryoEM examination. AD and CTE are classified into 3R+4R type [ ]. PiD is classified into 3R type. CBD, AGD, and PSP are classified into 4R type. The 4R types are divided into two classes. The PSP tau filament fold comprises threelayered core regions, whereas filamentous structures from CBD and AGD brains are fourlayered folds.",yes
PMC4648684,Figure_2,oa_package/1a/df/PMC4648684.tar.gz,"['Similar analyses here revealed that IL-36 KO mice had diminished dermal infiltration by neutrophils and fewer epidermal microabscesses than wild type mice ( and ', 'No differences between wild type and either IL-36 or IL-36 deficient mice could be detected ( and ', 'IL-36 , but not IL-36 or IL-36 , is required for neutrophil recruitmentWild type (a and b), IL-36 / (a), IL-36 / (a) and IL-36 / (b) mice were treated with imiquimod for 4 days as described in .']","Figure 2 IL-36, but not IL-36 or IL-36, is required for neutrophil recruitment Wild type ( and ), IL-36 ( ), IL-36 ( ) and IL-36 ( ) mice were treated with imiquimod for 4 days as described in . Skin was collected the day after the last imiquimod application and examined by H&E staining or immunohistochemistry for neutrophils (Ly-6G/Ly-6C). Red arrows indicate Munros microabscesses (clusters of neutrophils) at the top of the epidermis. Black arrows indicate individual neutrophils within the dermis. Representative images from the experiments also analyzed in and are shown. Scale bars = 50 m.",yes
PMC10598507,Figure_1,oa_package/40/04/PMC10598507.tar.gz,"['Later, an MRI of the brain revealed a single lesion in the right frontal lobe ().', '\nSekharLNFesslerRG\nStereotactic biopsy\nAtlas of Neurosurgical Techniques: Brain\n2nd Edition\nVolume 2\nNew York\nThieme Medical Publishers, Inc\n2016\n12\n24\n.']","Figure 1. Brain MRI in the transverse plane, T1-weighted post-gadolinium, showing a hypotense appearance of the cystic compartment and a ring-enhancing lesion, while the solid compartment of the tumor appears hypertense. There is also significant peritumoral edema present.",yes
PMC10258702,Figure_2,oa_package/6d/4c/PMC10258702.tar.gz,[],"Figure 2 patient before surgical treatment image taken in the outpatient clinic. image taken before the operation (a progression of HS is seen red colour), image taken after the surgical procedure (co-graft of ADM and STSG and rotation flap) pale STSG and pale rotation flap (ischemia) can be seen, image taken 4 days after the operation, image taken 2.5 months after surgical treatment (normal healing process), image taken 3 months after surgical treatment (fully healed)",yes
PMC8688409,Figure_2,oa_package/3a/c9/PMC8688409.tar.gz,['.'],"Fig. 2. (A) Histology of skeletal muscles from short-term survivors (surviving less than 1 week) showed inflammatory cell infiltration (arrows) consisting of macrophages/histiocytes, plasma cells and few lymphocytes, degenerating muscle cells with centralized nuclei (arrowhead) and necrotic myocytes (asterisk). (B) Myocardial sections did not display histopathological alterations evident at the light-microscopic level. (C) In pigs surviving for 3-4months, multiple skeletal muscles showed a similar spectrum of alterations as in A, but the lesions were more pronounced and advanced. (D) Myocardium did not show prominent changes at the light-microscopic level. (E) Skeletal muscle from the longest-surviving (8.5 months) boar #6790 displayed the changes described above accompanied by infiltration of eosinophilic granulocytes (rectangle). (F) At this age, obvious histological alterations of myocardium were revealed. Histology: paraffin sections, Giemsa staining. Scale bars: 50m. (G,H) Principal component analyses of proteome profiles from skeletal muscle (G) and myocardium (H) tissue samples of and WT pigs of different age groups (<1 week: =5, 4; 4months: =5, 4; >6months: =2, 2, respectively).",yes
PMC6786107,Figure_2,oa_package/7f/25/PMC6786107.tar.gz,['Radiological images of adrenal adenoma.'],"Figure 2 Radiological images of adrenal adenoma. a-c: Lipid rich typical adrenal adenoma. (a) Unenhanced CT, (b) Early phase on contrast enhanced CT, (c) Delayed phase on contrast enhanced CT. d-f: Lipid poor adrenal adenoma. (d) Unenhanced CT, (e) Early phase on contrast enhanced CT, (f) Delayed phase on contrast enhanced CT. g-i: Lipid poor adrenal adenoma. (g) Out-of-phase MR image, (h) in-phase MR image, (i) Chemical shift subtraction MR image. Arrows indicate the adrenal tumors.",yes
PMC11284650,Figure_4,oa_package/3d/3a/PMC11284650.tar.gz,"['After the diagnosis was confirmed, the patient was transferred to the Department of Hematology, and whole-brain radiation was administered (30 Gy/15 fractions) plus boost radiation (10 Gy/5 fractions) to the right temporal subdural space (A).', 'After radiotherapy, the subdural effusion decreased, and remission was maintained without reaccumulation (B and C).', 'FIG.']",FIG. 4. Postoperative radiation therapy with the achievement of good local control. An axial planning image for radiation therapy; boost at the right temporal region after whole-brain radiation therapy was performed. Axial CT obtained 4 weeks postradiation therapy with decreased subdural fluid collection. Contrast-enhanced MRI 6 weeks postradiation therapy also confirmed good local control.,yes
PMC11069329,Figure_2,oa_package/45/dc/PMC11069329.tar.gz,[],"Figure 2 Preoperative MRI T2 sequence, lumbar spinal cord, axial section showing intramedullary lesion with infiltrative character",yes
PMC3075833,Figure_4,oa_package/aa/1b/PMC3075833.tar.gz,"['[a b] Rajasekaran (2002) suggested stabilization of the spine posteriorly followed by anterior debridement and bone grafting in the active stage of the disease.', 'a-b26year old male presented with late onset paralysis due to conservatively treated childhood tuberculosis leading to significant kyphosis.']",Figure 4a-b 26year old male presented with late onset paralysis due to conservatively treated childhood tuberculosis leading to significant kyphosis. This was treated with posterior stabilization and internal gibbectomy,yes
PMC3369494,Figure_3,oa_package/e3/47/PMC3369494.tar.gz,"['Before mobilising the transport segment including number 25, the distractor was adapted to its stabilising plates with screws, and then the segment was mobilised using osteotomes ().', '002""/>Intraoral view of distractor.']",Figure 3 Intraoral view of distractor.,yes
PMC8607361,Figure_1,oa_package/ce/17/PMC8607361.tar.gz,"['Representative images of esophageal cancer on endoscopy (A), EUS (B) and PET scan (C).']","Figure 1 Representative images of esophageal cancer on endoscopy (A), EUS (B) and PET scan (C). EUS:endoscopic ultrasound; PET:positron-emission tomography.",yes
PMC11103984,Figure_2,oa_package/ad/fe/PMC11103984.tar.gz,"[' 2).', '', ' 2Computed tomography of the abdomen showing acute pancreatitis features']",Fig.2 Computed tomography of the abdomen showing acute pancreatitis features,yes
PMC10679589,Figure_4,oa_package/bd/1a/PMC10679589.tar.gz,['A 22-year-old female was diagnosed with bilateral ovarian dysgerminoma and peritoneal and lymphatic dissemination.'],"Figure 4 A 22-year-old female was diagnosed with bilateral ovarian dysgerminoma and peritoneal and lymphatic dissemination. Axial T1WI (a) and axial T2WI (b) show large, multilobulated, heterogeneous bilateral tumor, with septa of low-SI on T1WI and T2WI (thin arrows, a, b), some of them associated with edematous component. Hemorrhagic areas with high-SI on T1WI are observed (arrowhead, a), as well as multiple cystic areas, low-SI on T1WI and high-SI on T2WI (thick arrows, a, b). Axial gadolinium-enhanced image (c) revealing heterogeneous contrast enhancement of the tumor, with several enhancing and thickened septa (thin arrows). Axial and coronal T2WI (d, e) also reveal exuberant peritoneal metastases (asterisks). Note the presence of metastases in the left abdominal wall, anterior to the abdominal wall muscles, which were externalized through the entrance port of diagnostic laparoscopic surgery (stars, a, b, e). T1WI, T1-weighted imaging; T2WI, T2-weighted imaging; SI, signal intensity.",yes
PMC9005682,Figure_1,oa_package/bc/b7/PMC9005682.tar.gz,[],FIGURE 1 Sections of healthy human palmar aponeurosis processes for the detection of S100 protein (S100P) show the presence of small nerve bundles (thick arrows) and free nerve endings (thin arrows). Scale bar: 50m. bv: blood vessel,yes
PMC11622091,Figure_3,oa_package/e9/90/PMC11622091.tar.gz,['\nAngiographic evaluation of the biliary drainage tube.'],Figure 3 A: No contrast medium was infused; B: Contrast medium was injected through the drainage tube for visualization. a: Right hepatic duct; b: Left hepatic duct; c: Ductuli hepaticus communis; d: Common bile duct; e: Gallbladder stump; f: Cystic duct; g: Lower common bile duct stenosis.,yes
PMC11245804,Figure_1,oa_package/94/32/PMC11245804.tar.gz,"['00%)\nAn example of two data points extracted from a single exam, which consisted of two CR images depicting the right ankle and foot.', ') translation\nAs shown in ', ' 1, each DICOM file consists of two main parts: the raw image (pixel data) and the metadata describing the image (DICOM tags located in the file header).', '1Distribution of modalities across the entire 25 million DICOM filesNot all exams resulted in a recorded diagnosis, meaning that diagnoses of some of the performed exams were empty or null.']","Fig. 1 An example of two data points extracted from a single exam, which consisted of two CR images depicting the right ankle and foot. Given that these images were acquired as part of a single examination, they were linked to the same diagnosis, which was written in the Croatian ( ) language. An excerpt from the diagnosis is given in the illustration, along with its English ( ) translation",yes
PMC6973805,Figure_3,oa_package/07/00/PMC6973805.tar.gz,['3).'],Fig. 3 Enema showed narrowing from the descending colon to the rectum (white arrows),yes
PMC6868282,Figure_3,oa_package/12/a4/PMC6868282.tar.gz,"[' 3).', 'Fluorescent lemur tyrosine kinase 2 (LMTK2) immunohistochemistry (IHC) intensity plots of control (CNT), Alzheimer s disease (AD) and neocortical Lewy body disease (LBD) groups.']","Figure 3 Decreased labelling of lemur tyrosine kinase 2 (LMTK2) in Alzheimers disease (AD) as compared to neocortical Lewy body disease (LBD) and control brains. In the control and neocortical LBD the LMTK2 immunopositivity is more intense with chromogenic (brown)(Panels A,B) and fluorescent (red) technique (Panels E,F), respectively, compared to AD (Panels C,G). IHC negative slides (without application of primary anti-LMTK2 antibody) are also presented (Panels D,H). [The protein was visualized by 3,3-Diaminobenzidine (DAB) chromogen and Alexa Fluor 594 fluorescent dye. Nuclear counterstains were haematoxylin and 4,6-diamidino-2-phenylindole (DAPI) on chromogenic and fluorescent slides, respectively. Scale bar: 50m].",yes
PMC4151867,Figure_5,oa_package/b9/91/PMC4151867.tar.gz,"['Benazepril treatment inhibited the high glucose induced TGF- 1, ILK and -SMA expression at 48 h.']","Figure 5 The TGF- , ILK and -SMA mRNA expression in NG, MG, HG and ACEI were determined by real-time RT-PCR at 48h. Western blotting determined ILK and -SMA protein expression in the NG, MG, HG and ACEI at 48h. A representative western blot analysis. Densitometric quantification of the corresponding bands is performed using Kodak 1D Image software. Values are meansSE and are expressed relative to the control. =6. *, <0.05 vs control; #, <0.05 vs DN.",yes
PMC9487537,Figure_4,oa_package/32/3b/PMC9487537.tar.gz,"['22 versus the control) (B).', 'In PBLs, CSY5669 and AZM reduced bacterial loads to a similar degree (82% 2% and 82% 3% relative to the control) (B).', 'With this experimental setup, both CSY5669 and AZM significantly reduced intracellular bacterial loads compared with controls, with CSY5669 showing a significantly greater effect than that of AZM (55% 15% and 29% 22%, respectively) (C).', 'In MDMs, ROS production was unaffected by either treatment (D).', '006 versus control in monocytes and neutrophils that were gated separately, respectively) (E and F).', 'Spontaneous ROS production, which ultimately leads to oxidative stress, was not affected by either AZM or CSY5669 in either MDMs or PBLs (D to F).', 'Neither CSY5669 nor AZM affected phagocytosis (G).', 'CSY5669 did not affect the total number of phagosomes but surprisingly reduced the colocalization of MRSA with phagolysosomes, while AZM had no effect (H).', 'Stimulating neutrophils with MRSA induced neutrophil activation, as measured by CD18, and degranulation of all three granules (I).', 'The production of TNF- and IL-6 by macrophages was not significantly affected by CSY5669 (J).', 'In PBLs, TNF- production was also unaffected, but MRSA-induced IL-6 production was significantly reduced by both AZM and CSY5669 (K).']","FIG 4 Effect of CSY5669 on antimicrobial and inflammatory responses by monocyte-derived macrophages and blood leukocytes. Monocyte-derived macrophages (MDMs, =5) or peripheral blood leukocytes (PBL, =4) were infected with MRSA and subsequently incubated overnight (MDMs) or 6h (PBL) in the presence of 1M AZM or CSY5669, after which intracellular bacterial numbers were determined (A) and presented, relative to DMSO controls (B). Alternatively, PBLs were pretreated with AZM or CSY5669, washed, and subsequently infected and incubated with MRSA for 6h (C). MDMs ([D], =3) or PBLs (gated on viable CD14 monocytes [E] or on viable CD66b neutrophils [F], =4; for the gating strategy used, see Fig. S3 in the supplemental material) were incubated with heat-killed MRSA for 60min in the presence of ROS-reactive dye, after which cells were measured with flow cytometry. Monocyte-derived macrophages (MDMs) were incubated with heat-killed, FITC-labeled MRSA for 30min and measured with flow cytometry ([G], =4). MDMs were infected with viable MRSA and incubated for 120min, after which cells were fixed and measured using confocal microscopy to determine the phagosomal abundance (number of lysotracker organelles per cell) or the colocalization of phagolysosomes with bacteria ([H], =4). Isolated neutrophils were incubated for 60min with heat-killed MRSA, after which the expression of degranulation markers (CD63:azurophilic/primary granules, CD66b: specific/secondary and tertiary granules, CD11b: secretory vesicles, CD18:general activation marker) was determined ([I], =4). Expression levels were normalized to unstimulated DMSO-treated controls. MRSA-induced cytokine release was determined in MDMs ([J], overnight incubation, =5) and PBLs ([K], 4h incubation, =6). Data are presented as mean standard deviation, with each dot representing (the average of) individual experiments. Statistical significance was tested using a one-way analysis of variance with Tukeys test. *, < 0.05; **, < 0.01; ***, < 0.001; ****, < 0.0001.",yes
PMC1526630,Figure_5,oa_package/a2/b3/PMC1526630.tar.gz,['Immunohistochemistry (IMH) and in situ hybridization (ISH) of synovial tissues for BMP-5.'],"Figure 5 Immunohistochemistry (IMH) and hybridization (ISH) of synovial tissues for BMP-5. Histomorphological distribution of BMP-5 is comparable to that of BMP-4 (Figure 4). Original magnifications: immunohistochemistry (IMH): rheumatoid arthritis (RA), osteoarthritis (OA), normal donors (ND) 20; hybridization (ISH): RA, OA, ND 40. BMP, bone morphogenetic protein.",yes
PMC7863717,Figure_3,oa_package/67/4f/PMC7863717.tar.gz,"['Parallel placementParallel metal biliary stent placement () in cases of previous occlusion can be performed with side-by-side or stent-in-stent insertion; the latter configuration is complex, the placement of more than two stents is rarely reported, and retreatment is challenging or impossible(15).', '\nAn 80-year-old female patient, with locally advanced adenocarcinoma of the pancreatic head, who presented with cholangitis.']","Figure 3 An 80-year-old female patient, with locally advanced adenocarcinoma of the pancreatic head, who presented with cholangitis. She underwent an endoscopic procedure to implant a metal stent (WallFlex; Boston Scientific, Marlborough, MA, USA). At two weeks after the procedure, her jaundice worsened. Computed tomography of the abdomen revealed marked dilatation of the left intrahepatic biliary tract-the consequence of an improperly positioned biliary stent, selective in the right hepatic duct by upward migration, occluding the confluence with the left hepatic duct ( ). Another endoscopic procedure was performed to remove or reposition the stent. After its distal end had been captured, it was possible to crack the ring and the coating of the stent without moving it. Cholangiography showed peri-stent contrast retention and positioning of a different metal stent (Viabil; W.L. Gore, Flagstaff, AZ, USA) parallel to the WallFlex stent ( ). Postprocedure cholangiography showed satisfactory biliary drainage into the duodenum ( ).",yes
PMC11177727,Figure_2,oa_package/2c/e2/PMC11177727.tar.gz,"['A repeat echocardiogram revealed a septal bounce with annulus reversus and a plethoric IVC; the GLS was 19 (), findings suggestive of CP.', '1177_23247096241248969-fig2"" position=""float""/>.']","Figure 2. Panel A. 2D echocardiography, parasternal short axis view showing respiratory related septal shift. Panel B. Shows a dilated inferior vena cavae of 2.33 cm with less than 50% respiratory variation. Panel C and D. Tissue Doppler imaging demonstrated higher medial than lateral early diastolic annulus velocities (17.1 cm/s and 14.6 cm/s, respectively). Evidence of annulus reversus. Panel E. Speckle tracking imaging, global longitudinal strain was 19% (Normal range: 16% to 22%).",yes
PMC11587650,Figure_7,oa_package/69/48/PMC11587650.tar.gz,"[' 7A).', ' 7B, C).', ' 7D) or LAMP1 immunofluorescence (', ' 7E) were increased, while degradative capacity (', ' 7F) and neuritic trafficking velocity (', ' 7G) were reduced.', ' 7H, I) however lysosomal GCase activity was reduced by 20% in CTSB-KO neurons (', ' 7J, K).', ' 7L, M).', '', '0001CTSB deficiency promotes synuclein pathology in human dopaminergic neurons and midbrain organoidsCompared to parental control, CTSB-KO DA neurons were found to have modestly elevated levels of endogenous -syn (']","Fig.7 CTSB knockout impairs lysosome function in dopaminergic neurons. Representative immunofluorescent images from high-content confocal imaging of iPSC-derived DA neurons differentiated from control or CTSB-KO iPSCs and stained for Map2, tyrosine hydroxylase (TH) and -syn. Representative western blot depicting CatD protein levels in control and CTSB-KO neurons. Representative western blot depicting CatL protein levels in control and CTSB-KO neurons. High-content imaging representative image and quantification of lysotracker fluorescence in DA neurons. LAMP1 immunofluorescence per cell in Map2-positive DA neurons. Lysosomal degradative capacity measured by fluorescence intensity of DQ-BSA fluorogenic probe 24-h after dye loading. Quantification of lysosome velocity in neurites measured by live-cell confocal imaging and quantified using TrackMate. Points represent individual quantified image fields derived from 6 independent experiments. , Representative western blot and quantification of GCase and actin in DA neurons. Quantification of PFB-FDGlu fluorescence per cell in DA neurons ) Quantification of the slope of PFB-FDGlu fluorescence versus time. , Representative saposin C (sapC) western blot and quantification relative to actin loading control. T-tests, ** <0.01, *** <0.001, **** <0.0001",yes
PMC10404368,Figure_2,oa_package/f9/9c/PMC10404368.tar.gz,"[' 2).', '5394 262ACTH, Adrenocorticotropic hormone; TSH, Thyroid-stimulating hormone; LH, Luteining hormone; FSH, Follicle-stimulating hormone; GH, Growth hormone; IGF-1, Insulin-like growth factor-1; OGTT, Oral Glucose Tolerance TestEndoscopic endonasal approach to remove pituitary macroadenoma.', 'C The normal pituitary gland was identified and kept intact without injuryImmunohistochemical staining for pituitary macroadenoma.']","Fig. 2 Endoscopic endonasal approach to remove pituitary macroadenoma. The dura was incised, and the tumor was exposed. The tumor was yellow and soft. Extracapsular dissection between tumor pseudocapsule and diaphragma sella was performed. The normal pituitary gland was identified and kept intact without injury",yes
PMC10118781,Figure_2,oa_package/8a/8b/PMC10118781.tar.gz,"['CT imaging demonstrating improvement of tracheal compression and lumen reduction.', 'Patient remains asymptomatic with stable metastatic disease after 52 months of diagnosis (C).']",Figure 2 CT imaging demonstrating improvement of tracheal compression and lumen reduction. ( ) Initial scan. ( ) Scan after 12 months of sorafenib. ( ) Last scan after 4 years of diagnosis.,yes
PMC10476693,Figure_3,oa_package/2a/a5/PMC10476693.tar.gz,['CT scan with axial view showing percutaneous drainage of left psoas abscessHe was transferred to the medical intensive care unit for further management.'],Figure 3 CT scan with axial view showing percutaneous drainage of left psoas abscess,yes
PMC6609783,Figure_1,oa_package/ba/14/PMC6609783.tar.gz,"['In his past medical history, he received surgical treatment for primary tongue cancer, at the age of 19 years, and the pathological diagnosis was squamous cell carcinoma ().', 'Hematoxylin-eosin staining.', '']","Fig. 1 Hematoxylin-eosin staining. Low-power field; primary tongue squamous cell carcinoma, High-power field.",yes
PMC5775598,Figure_1,oa_package/e5/ee/PMC5775598.tar.gz,"[' 1a) and rarefaction of the eyebrows.', '1b).', '1c), dysphonia, and severe muscle atrophy in the extremities.', 'Tyr4195fs)General clinical dataAge (years)2825933GenderFFMMConsanguinityPresentPresentPresentAbsentPregnancyFull term/ UneventfulFull term/ UneventfulNANASkin involvement-skin blistering age onsetNeonatalNeonatalBirthNeonatal-skin blistering evolutionDiminished with ageDiminished with ageNAAugmented every summer-nailPresentPresentPresentPresent-teethPresentPresentPresentPresent-focal plantar keratodermyAbsentAbsentAbsentAbsentMuscle involvement-age onsetAdolescenceAdolescenceInfancyInfancy-evolutionMuscle atrophy 28y unable to perform activities of daily livingMuscle atrophy 20y unable to walk long distances or climb stairsMajor difficulties in walking at 9yMuscle atrophy 20y unable to walkMucosa involvement-oral mucosal blisteringPresentAbsentPresentPresent-hoarsnessPresentPresent (adolescence)NANA-laryngeal webNDNANANA-urethral strictureAbsentNAPresentNARecurrent infectionsUpper respiratory tract (childhood)PresentNANANANA Not available, ND Not determinedClinical features in the 28-year-old female with epidermolysis bullosa simplex with muscular dystrophy and diffuse alopecia.', 'a Diffuse alopecia of the scalp, (b) Sparse and hemorrhagic blistering of the hand and onychodystrophy, and (c) Oral cavity abnormalities, such as caries and enamel hypoplasiaTo identify the pathogenic mutation, we performed PCR amplification of all coding exons and exon intron boundaries of the PLEC gene (isoforms 1a, 1b, and 1c), followed by bidirectional Sanger sequencing, after obtaining the patient s informed consent (the study was conducted according to the Declaration of Helsinki Principles).']","Fig. 1 Clinical features in the 28-year-old female with epidermolysis bullosa simplex with muscular dystrophy and diffuse alopecia. This patient is originated from the Azorean island of So Miguel (Portugal). Diffuse alopecia of the scalp, ( ) Sparse and hemorrhagic blistering of the hand and onychodystrophy, and ( ) Oral cavity abnormalities, such as caries and enamel hypoplasia",yes
PMC5306191,Figure_3,oa_package/4c/fd/PMC5306191.tar.gz,"[' 3.', '', 'A general classification of the biological roles of glycans is presented, emphasizing the roles of organism proteins in the recognition of glycans; from Varki and Lowe (2009), with permission\nBacked by exemplary references, special aspects are highlighted:Impact on the physicochemical properties of the glycoprotein molecule.']","Fig.3 Biological roles of glycans. A general classification of the biological roles of glycans is presented, emphasizing the roles of organism proteins in the recognition of glycans; from Varki and Lowe ( ), with permission",yes
PMC5996926,Figure_7,oa_package/30/f6/PMC5996926.tar.gz,[' TC2 differentiation is increased in colonic mucosa of children with colitis.'],"Figure 7 T 2 differentiation is increased in colonic mucosa of children with colitis. T 2 differentiation in nave CD8 T cells isolated from children with non-inflammatory bowel disease and non-infectious pediatric idiopathic colitis (PIC). Representative endoscopic images of colonic mucosa from control children and children with PIC. Intracellular expression of IL-4 and IFN- in CD8 intraepithelial lymphocytes from colonic mucosa following stimulation with PMA and ionomycin. -value was calculated by MannWhitney test. Expression of CD8, activated caspase-3, and IL-4 in paraffin-embedded sections of colonic biopsies. Data are representative of >3 independent experiments.",yes
PMC10436745,Figure_3,oa_package/65/ed/PMC10436745.tar.gz,['3-year follow-up of the area after excision of PCOC noted for normal healing and no recurrence of the lesion.'],Figure 3 3-year follow-up of the area after excision of PCOC noted for normal healing and no recurrence of the lesion.,yes
PMC10668932,Figure_17,oa_package/cb/ea/PMC10668932.tar.gz,[],"Fig. 17. A 43-year-old female with medial sided ankle pain. Coronal T2-weighted (T2W) fat-suppressed (FS) MR image through the ankle demonstrates thickening and increased signal within the medial plantar nerve (arrowhead), which can be seen in the setting of Joggers foot. Short-axis T2W FS MR image of the forefoot at the level of the metatarsal bases demonstrates denervation edema within the abductor hallucis muscle (arrowhead)",yes
PMC9694579,Figure_6,oa_package/64/73/PMC9694579.tar.gz,"['Administration of C9 had no adverse impact on weight loss or total circulating leukocytes (A,B).', '014; C).', 'As such, approximately 50% of vehicle-treated mice had mild and 50% minimum inflammatory cell infiltration while C9-treated mice showed 50% mild, 25% minimum, and 25% absent (D).', '0001) in C9-treated mice in comparison with vehicle-treated littermates (D).', 'Panels F-I are representative of E.', '0067; J).', 'YFV 17D presents with self-limiting disease and mild pathology in A129 mice during the first week of infection [35,36] as was evidenced by no mortality or significant change in body weight or total leukocyte counts post-infection (A,B).', 'Administration of C9 also had no significant impact on these parameters, suggesting no adverse effect on mice (A,B).', 'Importantly, C9 offered significant protection against YFV-induced liver damage and inflammation as well as significantly reduced viral burden in the liver (C J).', 'In vivo anti-YFV activity of C9 in A129 mice.']","Figure 6 In vivo anti-YFV activity of C9 in A129 mice. Adult (810 weeks old) A129 mice were infected i.v. with 10 PFU/100 L of YFV virus (YFV strain). Daily intraperitoneal injections of C9 (10 mg/kg) were started 1 hr post-infection and continued until 3 days post-infection (dpi). Mice were euthanized 6 hr after the last treatment with vehicle or C9 on day 3 post-infection. Blood and tissues were collected and processed for further analysis. Groups were composed of six mice, three males, and three females. ( ) Body weight change post-infection was assessed by two-way ANOVA plus Sidaks multiple-comparison test. Data are shown as mean SEM, = 6. ( ) Changes in the number of circulating total leukocytes 3 days post-inoculation, represented as box plots. Data from each group were compared with those from the mock or mock + C9 groups by one-way ANOVA plus Dunnetts multiple-comparison test data shown as mean SEM, = 6. ( ) Estimation of liver dysfunction analyzed by serum concentration of alanine aminotransferase (ALT). Results are expressed as U/L. Statistical differences among groups were assessed by one-way ANOVA plus Tukeys post hoc test data shown as mean SEM, = 6. ( ) Percentages of mice according to the grade of inflammatory cell infiltration in the liver. ( ) Histopathological assessment in relation to overall inflammatory score. Comparisons between mock and mock + C9 and infection groups were carried out by Kruskal-Wallis plus Dunns post hoc test data shown as mean SEM, = 6. ( ) Representative H and E-stained liver slices of mock ( ), mock + C9 treated ( ), YFV + vehicle ( ), and YFV + C9-treated mice ( ) at 3 dpi. Scale bar: 100 m. ( ) Quantitative RT-PCR analysis of YFV viral load in the mice livesr tissue 3 days after YFV inoculation. Data shown as mean SEM, = 6; * < 0.01 in comparison to the non-infected (mock) group,; # < 0.01 when comparing YFV + C9 to YFV group using unpaired test.",yes
PMC8662811,Figure_3,oa_package/ce/cb/PMC8662811.tar.gz,[],"Figure3 Lung tissue histopathology of TCR and WT mice after adoptive transfer of CD4+ T cells. Lung histopathology of uninfected, M.tb-infected TCR , and WT mice before and after CD4+ T-cell transfer. The left panel shows KO mice lung sections, and the right one shows WT mice lung sections. Mononuclear cells are shown with green colored arrow and foamy macrophages are shown with blue colored arrows. Black colored arrow show lymphocytes.",yes
PMC10669759,Figure_1,oa_package/45/ca/PMC10669759.tar.gz,"['Precursor-Type IDC-PAlthough exceedingly rare, IDC-P has been observed in association with only GG1 PCa, distant from high-grade PCa, or even without a concurrent PCa () in rare RP specimens; and in these scenarios, IDC-P may represent a precursor lesion in prostate carcinogenesis rather than retrograde spread of high-grade PCa into pre-existing ducts and acini (see discussion below).', '000000000000034825517949Isolated IDC-P without concomitant prostate cancer in a prostate biopsy (A,B).']","Figure 1 Isolated IDC-P without concomitant prostate cancer in a prostate biopsy ( , ). The IDC-P glands have residual basal cells positive for basal cell markers (brown stains) and are also positive for AMACR (red stain) ( ). Isolated IDC-P is associated with unsampled GG 2 PCa in the majority of cases, although no invasive or only GG1 PCa is found in subsequent RPs in approximately 10% of cases.",yes
PMC5930234,Figure_2,oa_package/2e/3f/PMC5930234.tar.gz,['\nCT angiogram transverse views A D.'],Figure 2 angiogram transverse views AD. Red arrow indicating the PortACath entering the right subclavian artery.,yes
PMC6424673,Figure_1,oa_package/9e/96/PMC6424673.tar.gz,"['On fundus examination, about disc area in size yellowish-white color a laser burn zone was observed in the superior foveal region, involving the central foveola of the left eye, while the right eye was normal ().', 'Color fundus photography demonstrating yellowish-white color a laser burn zone in the central of the left eye.']",Fig. 1 Color fundus photography demonstrating yellowish-white color a laser burn zone in the central of the left eye.,yes
PMC8609285,Figure_1,oa_package/58/23/PMC8609285.tar.gz,"['To analyze cytoplasmic staining alone, an option would be to use Cytoplasm: DAB OD mean The measurement map tool, as shown in , was utilized to visualize stain intensity across the annotations and was used to adjust the GPR18 intensity threshold parameters.', '2Single thresholdNoVSM: Vascular smooth muscle, EVT: Extravillous trophoblastQuPath measurement map tool.']",Figure 1 QuPath measurement map tool. (a) QuPath measurement map tool being used to adjust staining threshold parameters. (b) Representative images show how QuPath's measurement map tool was used to visualize individual cell staining intensities for the indicated placental tissues,yes
PMC10708970,Figure_20,oa_package/2b/4a/PMC10708970.tar.gz,[],Fig. 10 Imagem radiogrfica em Saltzman com utilizao da Caixa.,yes
PMC6262647,Figure_1,oa_package/8f/04/PMC6262647.tar.gz,"['Eccentricity from the foveal center point also was calculated for each MA ().', 'Registering of microaneurysms on AOSLO imaging with wider-field infrared (IR) and SDOCT images.']","Figure 1 Registering of microaneurysms on AOSLO imaging with wider-field infrared (IR) and SDOCT images. The in the IR image ( ) indicates the location of the AOSLO scan ( ), the the location of the SDOCT scan ( ). : in IR and SDOCT image = 200 m; in AOSLO image = 100 m. *Center of fovea.",yes
PMC4908263,Figure_1,oa_package/c2/21/PMC4908263.tar.gz,"['CFTR also controls potassium (K+) transport by regulating the renal outer medullar potassium channel (ROMK) () [29].', '0-03426187659302172The role of CFTR in regulating additional ion channels.']","Figure 1 The role of CFTR in regulating additional ion channels. CFTR regulates many ion channels. CFTR primarily functions as a Cl channel. However, it also has a role in regulating the transport of K through renal outer medullar potassium channel (ROMK2). ROMK2 interacts with the intracellular cytoplasmic nucleotide-binding domains 1 (NBD1) and the regulatory (R) domain. CFTR can regulate the activity of outwardly rectified Cl channel (ORCC) through the binding of ATP to the purinergic receptor (PY2R). CFTR can also inhibit ENaC, therefore regulating Na transport into the cell.",yes
PMC7385689,Figure_3,oa_package/6a/72/PMC7385689.tar.gz,"['50) ().', '.']","Figure 3. Correlation of Myocardial Measures With Time From Coronavirus Disease 2019 (COVID-19) Testing There was no significant correlation with duration between the positive test for COVID-19 and the measures (native T1: =0.07; =.47; native T2: =0.14; =.15; high-sensitivity troponin T: =0.07; =.50). The trend line indicates the linear regression trend, and the shaded area indicates 95% CIs of the mean.",yes
PMC3650586,Figure_5,oa_package/66/4d/PMC3650586.tar.gz,"['To examine the mobility of microglia on surface-bound A , we prepared wells uniformly coated with surface-bound A 42 fibrils and tracked individual microglia cells moving on these surfaces (a).', 'mm 2, consistent with the possibility that surface-bound A 42 inhibited microglial migration (b).', 'cell 1) (c).', 'Microglial co-localization with patterned A To further understand how microglia target surface-bound A rich plaques, we patterned surface-bound A 42 in both oligomeric and fibril forms on wells at various concentrations by using PDMS stencils of 2 mm holes (d, Supplementary ', 'mm 2 on the eighth day (e f).', 'Microglial recruitment and accumulation in the presence of soluble and patterned A To reconstruct the A microenvironment analogous to that encountered by microglia near plaques, a core of fibrillar A surrounded by a halo of soluble A likely oligomers in the AD brains, we combined patterned surface-bound A 42 by using PDMS stencils of 1 mm holes and the gradient of soluble A 42 in the microfluidic platform (g, Supplementary ', 'mm 2 only (h i).', 'org/1999/xlink"" xlink:href=""srep01823-f4""/>Microglial co-localization on surface-bound A alone and in combination with soluble A .']","Figure 5 Microglial co-localization on surface-bound alone and in combination with soluble . (a) Microglial cells were cultured in wells uniformly coated with fibrils and tracked individually. (b) The fibrils induce the simultaneous decrease of the mobility and the viability of microglia, proportional to increased concentrations of the fibrils. (c) The level of secreted MCP-1 is elevated by about two-fold on surfaces coated with oligomers and fibrils of 9ng.mm compared to an uncoated surface. (d) Fluorescent image visualized co-localized microglia in red on a spot of fibrils at 90ng.mm in green. Co-localization of microglia on the patterned , becomes more effective as the concentration increases with 1.5-fold enrichment on oligomers (e) and 2-fold on fibrils (f) at 90ng.mm on a day 6, respectively. (g) Fluorescent image visualized co-localized microglia on fibrils at 90ng.mm combined with oligomers at 23ng.mL in a microfluidic platform. Co-localization is 1.6-fold on fibrils alone (h) compared to 2.5-fold on fibrils at 90ng.mm in combination with a gradient of oligomers at 23ng.mL (i). Scale bars, 1mm. (Student's t-test. * P < 0.1, ** P < 0.05 with respect to no ). = 2 in (a), (b), (e), (f) and = 2, 2,000 in (h), (i) for each condition. Data represent mean s.e.m.",yes
PMC4599331,Figure_3,oa_package/93/cf/PMC4599331.tar.gz,"['(A, B) VRT(volume rendered technique) images showing the interventricular septal aneurysm (yellow circle) with its neck (white arrow).']",Figure 3 ( ) VRT(volume rendered technique) images showing the interventricular septal aneurysm (yellow circle) with its neck (white arrow).,yes
PMC9046743,Figure_4,oa_package/6c/58/PMC9046743.tar.gz,"['His posterior fusion had been performed without correction of severe scoliosis, so that his thoracic and lumbar spine were displaced to the far left side of his thorax and abdomen (figure 4).', 'Multiple views of the spine in a patient undergoing transforaminal spinal anesthesia.', 'Because the spine was so close to the skin on the left side of the torso (figure 4), 25-gage 5 cm pediatric non-cutting needles were used without an introducer.']",Figure 4 Multiple views of the spine in a patient undergoing transforaminal spinal anesthesia. (A) Planned transforaminal needle trajectory (dashed arrow) from an axial view from a prior CT scan. (B) Far left: lateral position of the spine in a coronal view from a prior CT scan. (C) Ultrasound view of the needle trajectory. (D) Lateral tunnel fluoroscopic view. (E) Anteriorposterior fluoroscopic view of a 25-gage 5cm non-cutting needle used for spinal anesthesia. Image included with patient consent.,yes
PMC11282557,Figure_1,oa_package/22/1f/PMC11282557.tar.gz,"['A pelvic ultrasound examination () disclosed the agenesis of the uterus with normal follicular ovaries and two fused ectopic kidneys in the pelvic cavity.', '.', '1177_2050313X241265047-fig1"" position=""float""/>.']","Figure 1. Axial Pelvic ultrasound images reveal (a) the lack of uterine tissues in the space between the bladder (blue asterisk) and the rectum (orange asterisk), (b) and (c) two normal ovaries (red arrows) in the iliac fossae and (d) fused kidneys in the pelvis.",yes
PMC6060824,Figure_4,oa_package/f8/74/PMC6060824.tar.gz,"['4a (mild changes of term partial-prolonged hypoxic ischaemic injury).', 'The interhemispheric fissure is narrow (curved arrow) and the hemispheres and gyri are closely apposed (dashed arrows)Pathologic brains also demonstrated characteristic regional prasagittal atrophy in with widening of interhemispheric fissures and sulci as well as ulegyria, i.', ' 4).']","Fig. 4 , Control patient with normal MR imaging at the age of 4years and accompanying 3D print. Coronal T1 weighted MRI at the level of the 3rd ventricle in a healthy child age-matched with those in Figs. and 4. The interhemispheric fissure is narrow (curved arrow) and the hemispheres and gyri are closely apposed (dashed arrow). 3D printed model of the brain. The interhemispheric fissure is narrow (curved arrow) and the hemispheres and gyri are closely apposed (dashed arrows)",yes
PMC11228643,Figure_2,oa_package/8d/10/PMC11228643.tar.gz,"['Magnetic resonance imaging revealed multiple formations within the presacral space and pelvic cavity, measuring about 87 100 mm, tending towards confluence ().', '(A-C) Magnetic resonance images taken approximately 3 months after surgery.', 'Molecular characterization showed KRAS G12A mutant, NRAS, BRAF wild type, and microsatellite stable (MSS) tumor.']","Fig. 2 (A-C) Magnetic resonance images taken approximately 3 months after surgery. (A) T2-weighted sequence in the sagittal plane. In the context of anterior rectal resection, there is the presence of multiple formations, tending to converge, with maximum dimensions of approximately 87 100 mm, in the presacral space and the pelvic cavity. These formations show infiltration of the puborectal sling on both sides, the posterior wall of the uterus, and the vaginal fornices, without a clear cleavage plane with the anterior wall of the sacral vertebrae. (B) T2-weighted sequence in the axial plane. Multiple lesions with the same characteristics have been documented in the deep layers of the anterior abdominal wall, the largest in image G with dimensions of 50 33 mm in the axial plane, attributed to peritoneal diffusion implants. (C) Diffusion-weighted sequence in the axial plane at high B values.",yes
PMC4158351,Figure_2,oa_package/66/70/PMC4158351.tar.gz,['Protein comparison between the left and right ventricles of each disease.'],Figure 2 Volcano plot of the entire set of proteins quantified in the left and right ventricles of AVS patients. Volcano plot of the entire set of proteins quantified in the left and right ventricles of CAD patients. Each point represents the difference in expression (log fold difference) between left and right ventricles plotted against the level of statistical significance. Solid lines represent1.2 fold difference and a significance level of P<0.05 (Students t-test). Proteins represented by (x) had less than1.2 fold difference or were not statistically significant. Proteins represented by a () had greater than1.2 fold difference and were statistically significant.,yes
PMC5620448,Figure_3,oa_package/52/b7/PMC5620448.tar.gz,"['Ultrasonographic images obtained at a local breast clinic were reviewed; accessory breast tissues surrounding hypoechoic masses were evident ().', 'Sonographic findings of invasive micropapillary carcinoma in axillary ectopic breast.']","Figure 3 Sonographic findings of invasive micropapillary carcinoma in axillary ectopic breast. (A) Ultrasound image obtained in a local breast clinic shows several, irregular hypoechoic masses in the right axilla. (B) Echogenic area with the same appearance as that of normal breast tissue compatible with ectopic breast (arrowheads) is seen around the masses.",yes
PMC6186080,Figure_2,oa_package/5c/a8/PMC6186080.tar.gz,"[' 2).', 'Clinical course after transplantation.', 'The IL-6 levels are currently maintained at 300 500 pg/ml, and the CRP and IgG levels are trending towards normal with no allograft rejectionThe patient s IL-6 levels decreased dramatically after transplantation and are now maintained at 300 500 pg/ml under immunosuppression with a triple-drug regimen (TAC, MMF and mPSL) with TCZ (', '2).', '2).']","Fig. 2 Clinical course after transplantation. Tapering of triple immunosuppression and sustained treatment with TCZ in an MCD transplant recipient. The IL-6 levels are currently maintained at 300500pg/ml, and the CRP and IgG levels are trending towards normal with no allograft rejection",yes
PMC3155835,Figure_3,oa_package/ad/16/PMC3155835.tar.gz,"['Photomicrograph of mucinous carcinoma of the breast, a cluster of tumor cells in a pool of mucin, H and E stain ( 20).']",Figure 3 .,yes
PMC6231880,Figure_2,oa_package/89/2d/PMC6231880.tar.gz,"['For these reasons, the pathologists were forced to place themselves too close to the dissection table to be able to take pictures of small anatomical details or close-ups of the injuries correctly ().', 'The forensic pathologist wears Google Glass and takes pictures of small anatomical details in a hands-free manner.', 'DiscussionPrincipal FindingsPotentially disruptive technologies such as Google Glass create excitement for the possible applications that they can have in health care; however, the use of new tools should be thoroughly evaluated and validated before applying them in the medical or biomedical fields.']",Figure 2 The forensic pathologist wears Google Glass and takes pictures of small anatomical details in a hands-free manner.,yes
PMC9443853,Figure_3,oa_package/af/48/PMC9443853.tar.gz,"['This radiographic pattern, however, may vary the cyst may be multilocular, associated with unerupted/impacted teeth, and several cysts may be present in the same patient17 ().', 'Multilocular traumatic bone cyst in the body of the left mandible; this image first suggested a tumor.']",Figure 3 Multilocular traumatic bone cyst in the body of the left mandible; this image first suggested a tumor. The cortical plate is preserved.,yes
PMC10710008,Figure_3,oa_package/6e/f4/PMC10710008.tar.gz,"['8%) patients (Figs.', '', '1177_02841851231191761-fig3"" position=""float""/>']","Fig.3. A 67-year-old woman with vertebral metastasis in T12 and L1 from kidney cancer. (a) Digitally subtracted and (b) unsubtracted anteroposterior angiography of the left 1st lumbar artery demonstrating a hypervascular area in the region occupied by the metastatic lesion and AKA (arrowhead) originating from T12 (asterisk) through anastomotic circulation between the left 1st lumbar artery and the homolateral 12th intercostal artery. AKA, artery of Adamkiewicz.",yes
PMC7906274,Figure_2,oa_package/05/96/PMC7906274.tar.gz,[' Echocardiogram.'],"Figure 2 Echocardiogram. A: Left ventricular wall thickness is demonstrated hereusing the parasternal long-axis view of the heart at end diastole. Septal thickness and posterior wall thickness are both measured at1.1 cm which is higher than the normal range of 0.6-0.9 cm for both in females. B: Filling pressures are ascertained using doppler data gathered across the mitral valve in the apical view. The E and the A refer to two distinct waves created by the passive and active filling of the left ventricle respectively. Our patient's E/A ratio is 1.2 which is in the normal range of 0.75 and 1.5 making this a pseudonormalfilling pattern. C: Measuring tissue doppler can provide the E/E' ratio which canhelp distinguish between normal and pseudonormal filling patterns. Our patient's E/E' shown here is 16 which is highly suggestive of elevated filling pressures. For reference, normal E/E' ratios are less than 8.",yes
PMC4701869,Figure_3,oa_package/60/87/PMC4701869.tar.gz,"['An ellipse of skin was taken to allow adequate primary closure with minimal dead space (see ).', 'Intra-operative photo of lesion being excised in a fashion to limit dead space.']",Fig. 3 Intra-operative photo of lesion being excised in a fashion to limit dead space.,yes
PMC5429112,Figure_4,oa_package/7f/3c/PMC5429112.tar.gz,['A.'],"Figure 4 . Proliferation of irregular, anastomosed clefts (HE,100x). . Atypical endothelium, with hyperchromaticnuclei, projecting into the lumen (HE, 400x).",yes
PMC5946008,Figure_4,oa_package/c1/50/PMC5946008.tar.gz,['Six randomly selected examples of generated sagittal radiographs of the lumbar spine.'],Figure 4 Six randomly selected examples of generated sagittal radiographs of the lumbar spine. input: label data provided as input; output: image created by the generative model; target: ground truth.,yes
PMC3564530,Figure_2,oa_package/26/7b/PMC3564530.tar.gz,"['BGIN weakly coprecipitated with a high molecular weight poly-Ub smear under steady-state conditions, which was greatly enhanced with MG132 (A).', 'To determine whether the CIN exon 2 module (BGIN574-677, which we refer to as CIN exon 2 from here on), was required for poly-Ub interaction, we precipitated GST-tagged BGIN deletion constructs and assayed for poly-Ub interaction in the presence and absence of MG132 (B).', 'BGIN constructs lacking the CIN exon 2 domain were unable to coprecipitate with poly-Ub, whereas the CIN exon 2 module alone demonstrated efficient poly-Ub interactions under steady-state conditions, with enhanced interactions upon MG132 treatment (B).', 'BGIN did not appear to be covalently tagged with degradatory-targeting poly-Ub linkages since BGIN immunoblots (GST blots) did not mirror higher molecular weight smears seen in duplicate Ub blots (A).', 'Similarly, we also observed little or no binding with mono/di-Ub chains, with incrementally improved binding with Ub4 to Ub7 chains (C).', 'Using recombinant purified GST-tagged Ub-binding domains immobilized to glutathione beads, we found that the CIN exon 2 domain exhibited much weaker binding to recombinant linear Ub5 chains in vitro (D).', 'In vivo, we found that only the CIN exon 2 domain exhibited robust interactions with a poly-Ub smear (E).']","FIGURE 2: BGIN interacts with poly-ubiquitin through the CIN exon 2 module. (A) HeLa cells were transfected with GST or BGIN-GST constructs and treated with dimethyl sulfoxide (DMSO) or 10 M MG132 or 17AAG for 5 h, and GST precipitates generated from lysates were immunoblotted for GST or Ub. (B) GST-tagged BGIN constructs were expressed in HeLa cells, and GST precipitates were generated from cells treated with DMSO or MG132 (5 M, 5 h). Precipitates were immunoblotted for GST or Ub. (C) Recombinant purified GST-Ub chains of the indicated lengths were incubated with recombinant purified CIN exon 2-GFP and precipitated with glutathioneSepharose. Right, Coomassie stain of the binding reaction input. (DF) Comparison of Ub-binding modules in vitro and in vivo. (D) A 20-g amount of recombinant GST-tagged Ub-binding domains immobilized on glutathioneSepharose was incubated with 20 g of his -Ub2 or Ub5, precipitated, and analyzed for GST/Ub by immunoblot. Right, Coomassie stain of binding inputs. (E) GST-tagged Ub-binding domains in pRK5 were transfected into HeLa cells, precipitated with glutathioneSepharose, and analyzed for GST/Ub. (F) Graphs represent relative Ub binding in vitro (top) and in vivo (bottom; highest value set to 1.0) from two experiments (mean SD). Saturated band intensity with Ub5 in vitro binding as indicated will diminish differences detected in CIN exon 2 interactions compared with other Ub-binding domains.",yes
PMC9886736,Figure_4,oa_package/d0/44/PMC9886736.tar.gz,"[' 4A, proteins involved with glycolysis, TCA cycle, and pyruvate metabolism were the top pathways affected.', 'Reprogram of glucose metabolism in SIRT3cKO heart.', '05To functionally examine if glucose metabolism is affected by the loss of SIRT3, we examined insulin-stimulated glycolysis in cardiomyocytes isolated from 10-month-old mice.', ' 4B).', ' 4C).', ' 4D).', ' 4E).', ' 4E).', ' 4F).', ' 4F).', ' 4G).', ' 4E) in SIRT3cKO hearts.', ' 4E) supports the conclusion that there is increased reliance on glucose as an energy source.', ' 4F G).']","Fig. 4 Reprogram of glucose metabolism in SIRT3cKO heart. Results of the pathways analysis over KEGG database of the significantly (WT =4, KO =6) changed proteins between SIRT3cKO vs WT. Only significant pathways (qFDR<0.05) are presented. Numbers inside the bars represent detected versus background proteins in the pathway. The ratio of increasing glycolytic flux in WT and SIRT3cKO cardiomyocytes in the presence of 5ug/mL insulin ( =3 per group for each experiment, 3 independent experiments). Relative abundance of insulin-regulated glucose transporter 4 (Glut4) in heart ( =46). Representative western blots of phosphorylated and total Akt ( ) and quantitation ( ) of total Akt and phospho: total Akt ratio in hearts from WT and KO mice ( =45). PDH activity in heart mitochondria from WT and SIRT3cKO mice ( =57 per group). Relative abundance of PDH-related proteins in heart from 10-month-old mice ( =46 per group). Representative western blots of phosphorylated and total PDH ( ) and quantitation ( ) of total PDH and phospho:total PDH ratio in hearts from WT and SIRT3cKO mice ( =45 per group). Data are presented as meanSEM and analyzed using 2-way ANOVA followed by Tukeys post hoc test ( , ) and unpaired Students test ( , ). * <0.05",yes
PMC7785558,Figure_2,oa_package/9d/cf/PMC7785558.tar.gz,"[' 2a) confirmed that -secretase inhibition enhanced the accumulation of APP-CTFs (C99 and C83) and blocked A peptide production in total extracts (', ' 2a, b) as well as in mitochondria-enriched fraction (', ' 2a, c) without affecting the level of full-length APP (', ' 2a c).', ' 2a c).', ' 2d, e).', 'APP-CTFs accumulate in mitochondria and trigger mitochondria structure alteration in cells.', '0001 versus vehicle ( ) using Mann Whitney testWe took advantage of this pharmacological approach and analyzed mitochondria structure using electron microscopy, and revealed that -secretase inhibitor-treated APPswe cells display spherical and fragmented mitochondria (colored arrowheads in representative images in ', ' 2f).', ' 2g and suppl.', ' 2h, i and suppl.', ' 2j and suppl.', 'a, online resource).', 'a, b, online resource), and reduces mitochondrial network 3D volume (Suppl.', 'a, c, online resource).', 'd, online resource).', 'e, online resource).', ' 2f j, suppl.', ' 2a c), the treatment with -secretase inhibitor enhanced C83 level in both C99 and control cells (']","Fig. 2 APP-CTFs accumulate in mitochondria and trigger mitochondria structure alteration in cells. SDS-PAGE (low and high exposures) of APP and its indicated metabolites in vehicle ()- or -secretase inhibitor- (-sec inh) (+) (5M, 20h) treated APPswe cells revealed in total cell extracts (Tot), and mitochondrial-enriched fraction (Mit). Full-length APP (APP), C99, and A detected with 6E10 antibody, and C99, C83, and AICD detected with APP-Cter antibody. Actin and Cox IV antibodies were used as loading controls for total and mitochondria-enriched fractions, respectively. Quantitative graphs of indicated proteins in total extracts ( ) and mitochondrial fraction ( ) expressed as meansSEM ( =5) versus controls (taken as 100%). Immunostaining of SH-SY5Y APPswe cells treated as in ( ) with APP-Cter or 6E10 antibodies (green) in combination with anti-HSP60 (red) used to stain mitochondria. Nuclei were labeled with DAPI. Images show merge of green and red signals reflecting the colocalization in yellow. Scale bars represent 10m. Electron microscopy ultrastructure of SH-SY5Y APPswe-treated as in ( ). Scale bars correspond to 2m. nucleus. Colored arrowheads indicate mitochondria classes as shown in Fig. b. Quantitative graphs of mitochondria classes ( ), and meansSEM of mitochondria area (m ) ( ), perimeter (m) ( ), and number/10 m ( ). The quantification was obtained in two independent experiments performed in duplicates in at least 20 different fields (>150 mitochondria). , *** <0.001, **** <0.0001, and non-significant using KruskalWallis test and Dunns multiple comparison post-test. **** <0.0001 versus vehicle () using MannWhitney test",yes
PMC7694731,Figure_4,oa_package/f1/b3/PMC7694731.tar.gz,"['Tc-99 DMSA renal scintigraphy showed a small-sized right kidney, with delayed excretion and differential function of right kidney was found to be 30% of the total [].', '(A-D)Coronal MPR reconstruction of CT urography and late phase Tc99 DMSA examination in the same patient (A) NCCT shows right PUJ calculus and nephrostomy tube; (B and C) excretory phase of CT urography shows good excretion in both the right (red arrows) and the left (blue arrows) ureters; (D) shows Tc99 DMSA scan which reveals delayed excretion and reduced size of right kidney, differential function found to be 30% on right side (green arrows).']","Figure 4( Coronal MPR reconstruction of CT urography and late phase Tc99 DMSA examination in the same patient (A) NCCT shows right PUJ calculus and nephrostomy tube; (B and C) excretory phase of CT urography shows good excretion in both the right (red arrows) and the left (blue arrows) ureters; (D) shows Tc99 DMSA scan which reveals delayed excretion and reduced size of right kidney, differential function found to be 30% on right side (green arrows).",yes
PMC7733456,Figure_4,oa_package/95/52/PMC7733456.tar.gz,"[' 4A) an image analysis macro (Supplementary Method) was used to form a Region of Interest (ROI) that covered much of the lesions in the tissue (', ' 4D), as computing limitations prevented the formation of a 3D ROI.', ' 4Ci,ii) within the tissue using subtraction of the background signal (', ' 4B).', ' 4E) and a RenyiEntropy automated thresholding was applied with the mean threshold generated across the stack (', ' 4F).', ' 4G).', 'Morphometric analysis of infection within lesions.', 'Using this method, it was possible to calculate morphometrics of the Mtb aggregates within the lesions within individual mice.', ' 4Hi) and a greater total volume of infection (', ' 4Hii).', ' 4Hiii) and larger surface area (', ' 4Hiv), while another metric, colony sphericity (', ' 4Hv), showed minimal differences.', 'Volumetric measurements 4 shows the steps that were taken to isolate the speckly pattern within the images which was shown to be specific for Mtb.', 'tif"">Supplementary .']","Figure 4 Morphometric analysis of infection within lesions. The steps of the image analysis pipeline are presented ( ). To begin the Mesoscope image ( ) of a H37Rv infected lung sample, with a zoomed-in image ( ) showing the infection as a speckly pattern through the tissue, is mathematically squared ( ) and the background subtracted ( ). A zoomed-in image ( ) of the yellow region of interest in ( ) shows the pattern that has been isolated. A region of interest is then calculated to cover the lesions only ( ) and applied to the isolated signal ( ). The individual Z slices are then constructed into a 3D stack, with a maximum intensity projection of this sample shown in ( ) and a zoomed-in image at a tilt shown in ( ). The average RenyiEntropy threshold calculated across Z Stack and Isosurfaces generated ( ) with the volume (m ) colour coded for visualisation. Examples of morphometric data include total number of volumes ( ), volume of infection within the granuloma ROIs ( ), colony volume ( ), Surface area ( ) and Sphericity ( ) in H37Rv and Kenyan isolates infected tissue with volume (individual mice shown with n500 volumes measured per stack) (Black=Kenyan 1521, Orange=Kenyan 3870, Pink=Kenyan 3894, Grey= strain H37Rv; Solid colour=BALB/c host strain and Patterned colour=F1 mice). Scale bar=20m ( , ) and Scale bar=500m ( , , , , ) unless otherwise presented.",yes
PMC8394175,Figure_2,oa_package/0f/56/PMC8394175.tar.gz,"[' shows some of the ultrasonographic images obtained during this study.', 'Ultrasonographic images.']","Figure 2 Ultrasonographic images. ( ) EHthe endometrial line is not clearly defined, discreet hyperechogenic aspect of the endometrium (endometrial thickness = 25.45 mm); ( ) EH2D Color Doppler. Dispersed endometrial vessels and irregular endo-myometrial junction can be observed; ( ) 2D Color Dopplerdispersed endometrial vessels, hyperechogenic endometrium without an endometrial line can be seen; ( ) echogenic aspect of the endometrial fluid. A lesion can be observed (polyp), with an implant base of less than 25% of the endometrial surface; ( ) endometrial adenocarcinoma. Irregular and infiltrative aspect of the endometrium especially in the endo-myometrial junction, endometrial thickness of 26.7 mm; ( ) 3D TUI (Tomographic Ultrasound Imaging) Image acquisition.",yes
PMC10518679,Figure_2,oa_package/82/86/PMC10518679.tar.gz,"[""Axial (A), sagittal (B), and coronal (C) contrast-enhanced abdominal CT performed 3 days after endoscopic drainage, showing reduction of the abscess's size (red asterisks)."", ""The patient's clinical condition and laboratory tests progressively improved and she was discharged 10 days after endoscopic drainage.""]","Fig. 2 Axial (A), sagittal (B), and coronal (C) contrast-enhanced abdominal CT performed 3 days after endoscopic drainage, showing reduction of the abscess's size (red asterisks).",yes
PMC6079392,Figure_4,oa_package/d5/8c/PMC6079392.tar.gz,"['Moreover, as shown in s 4(c) and 4(d), EtOH increased the protein expression of ECM markers, collagen 1 by 15% and fibronectin by 134% (p 0.', '003""/>Effect of T 4 on EtOH- and LPS-induced fibrogenesis.']","Figure 4 Effect of T 4 on EtOH- and LPS-induced fibrogenesis. Total protein was extracted from whole liver tissue from mice of various groups to determine the protein expression of (a) SMA, (b) PDGF- receptor, (c) collagen 1, (d) fibronectin, (e) MeCP2, and (f) PPAR by Western blot analysis. All values are means of triplicate experimentsSE and were corrected for loading differences after reprobing with -actin. < 0.05 versus control; < 0.05 versus EtOH; < 0.05 versus EtOH+LPS.",yes
PMC8848424,Figure_2,oa_package/dc/4c/PMC8848424.tar.gz,"['3%; see A) and Tr.', '7%; see B).', 'Simplified figure of the pulmonary trunk branches of the right upper lung: (A) Tr.']",Figure 2 Simplified figure of the pulmonary trunk branches of the right upper lung: (A) Tr.sup + A.asc; (B) Tr.sup + Tr.inf + A.asc.,yes
PMC11191378,Figure_1,oa_package/5e/3d/PMC11191378.tar.gz,['T2-weighted TSE sagittal MRI image of the post-operative patientOriginal MRI image of a patient who underwent the study.'],Figure 1 T2-weighted TSE sagittal MRI image of the post-operative patient Original MRI image of a patient who underwent the study. TSE- Turbo spin-echo; MRI - Magnetic resonance imaging; 1 - Neovaginal depth; 2 - Inlet of the pelvis (IOP); 3 - Inferior pelvic aperture (IPA); - Alpha angle,yes
PMC10531151,Figure_16,oa_package/1a/d8/PMC10531151.tar.gz,[],Figure 16 Sections of the ventral ( ) and dorsal ( ) horns of the cervical spinal cord from the proband Iba-1 were immunolabeled for localization of reactive microglia.,yes
PMC7678286,Figure_1,oa_package/a6/e2/PMC7678286.tar.gz,"[' 1).', 'MR images before surgery: MR images before SRS show irregular nidus with abnormal signal in the right parasellar region(a, b, c, d).', 'Another lesion appears on the right temporal lobe (i, j, k, l)Due to the location of the nidus, craniotomy risks, and total resection feasibility, the patient received single-fractionated SRS with a central dose of 24 Gy and a margin dose of 12 Gy.', ' 1).', ' 1).']","Fig. 1 MR images before surgery: MR images before SRS show irregular nidus with abnormal signal in the right parasellar region( , , , ).)MR images 8months after SRS show size of CSM is similar to that before treatment ( , , , ). MR images after admission show that the size of CSM was larger than that of 8months after SRS. Another lesion appears on the right temporal lobe ( , , , )",yes
PMC9632221,Figure_2,oa_package/f4/ca/PMC9632221.tar.gz,[],"FIGURE 2 Fibrosis and apoptotic cells in renal tissue. Mice were divided into the following groups: NC, mice treated with normal saline; DMSO, mice treated with DMSO; Model; DN mice; 5mg/kg, DN mice treated with 5mg/kg tanshinone IIA; 10mg/kg, DN mice treated with 10mg/kg tanshinone IIA; 25mg/kg, DN mice treated with 25mg/kg tanshinone IIA. (a) Fibrosis level of different groups was observed using Masson's staining. (b) Apoptosis cell number of different groups was observed using the TUNEL assay. *** <.001 vs. NC group; <.05, <.01, <.001 vs. Model group; <.05, <.01 vs. 5mg/kg group; <.05 vs. 10mg/kg group. DN, diabetic nephropathy; NC, negative control.",yes
PMC11521525,Figure_4,oa_package/c5/08/PMC11521525.tar.gz,"['The pathological examination established the presence of a teratoma ().', '.']",Fig. 4. Postoperative pathological examination established the diagnosis of conus medullaris teratoma.,yes
PMC11513694,Figure_1,oa_package/f7/85/PMC11513694.tar.gz,['Chest radiograph showing diffusely scattered multiple tiny nodules in bilateral lung fields suggesting miliary tuberculosis.'],Figure 1 Chest radiograph showing diffusely scattered multiple tiny nodules in bilateral lung fields suggesting miliary tuberculosis.,yes
PMC7599077,Figure_12,oa_package/eb/d3/PMC7599077.tar.gz,[],"Figure6 STING Inhibition Ameliorates Neurodegeneration and (A) Representative bright-field images demonstrate that H-151 (1M) prevents the death of iPSC-derived motor neurons from TDP-43-ALS patients 28days after terminal differentiation (scale bars, 50m). (B) Quantification of cell death in (A), as measured by LDH release assay. (C) Prp-TDP-43 mice were injected intraperitoneally (i.p.) with H-151 (210g) three times per week for 4weeks, starting at disease onset at day 110 of age. This treatment significantly diminishes proinflammatory cytokine gene expression in the cortex and spinal cord, as seen by qPCR (n= 3). (D) Neuron loss was imaged by cresyl violet staining of a coronal section (scale bars, 5mm). A representative image is shown, with selected magnified grayscale images highlighting cortical layer V neurons marked by a brown bar (scale bars, 200m). (E) Automated quantification of cortical layer V neurons from (D) (n= 6). (F) H-151-treated mice demonstrated improved performance in the rotarod test compared with DMSO-treated mice (n= 5). All animals studied here were males. Data are mean SEM. The p values were calculated using two-way ANOVA in (B) or unpaired t test between two groups in (C), (E), and (F). p< 0.05, p< 0.01, p< 0.001.",yes
PMC4339831,Figure_2,oa_package/a5/68/PMC4339831.tar.gz,"['Incidentally, a chest plain film revealed a large double-density opacity overlying the cardiac silhouette on PA projection and was too posterior on the lateral projection to be visualized ().', '001""/>The PA (a) radiograph of the chest reveals a double-density opacity overlying the cardiac silhouette which on lateral (b) radiograph does not appear to localize to the anterior thorax or any compartment of the mediastinum.']",Figure 2 The PA (a) radiograph of the chest reveals a double-density opacity overlying the cardiac silhouette which on lateral (b) radiograph does not appear to localize to the anterior thorax or any compartment of the mediastinum.,yes
PMC11633006,Figure_7,oa_package/f5/46/PMC11633006.tar.gz,"[' 7A) and the clinically relevant subgroup of ER-positive patients (', ' 7C).', ' 7B).', ' 7D).', '\nPrognostic performance of the predicted 11-gene set proliferation scoreEvaluation of the prognostic performance on recurrence-free survival (defined as the time to having a locoregional or distant metastasis, contralateral tumour or death) in the external SCAN-B dataset.', '(D) Forest plot from multivariable Cox proportional Hazard regression in ER-positive patients for the predicted 11-gene proliferation score based risk groups, adjusting for age, tumour size, lymph node status, grade and HER2 status\nSimilar analyses were performed using the MKI67 gene proliferation marker.']","Fig. 7 Prognostic performance of the predicted 11-gene set proliferation score Evaluation of the prognostic performance on recurrence-free survival (defined as the time to having a locoregional or distant metastasis, contralateral tumour or death) in the external SCAN-B dataset. Kaplan-Meier curve for all patients stratified by the predicted 11-gene proliferation score into high- and low-risk groups. Forest plot from multivariable Cox proportional hazard regression in all patients for the predicted 11-gene proliferation score based risk groups, adjusting for age, tumour size, lymph node status, grade, ER status and HER2 status. Kaplan-Meier curve for ER-positive patients stratified by the predicted 11-gene proliferation score into high- and low-risk groups. Forest plot from multivariable Cox proportional Hazard regression in ER-positive patients for the predicted 11-gene proliferation score based risk groups, adjusting for age, tumour size, lymph node status, grade and HER2 status",yes
PMC9484759,Figure_1,oa_package/f7/5c/PMC9484759.tar.gz,[],"FIGURE 1. (A) DWI shows hyperintensity at the left head of caudate nucleus (white arrow). (B) DWI shows hyperintense foci involving the left parietal region (white circles). (C) ADC shows corresponding hypointensity at the left head of caudate nucleus, in keeping with acute infarct. (D) ADC shows corresponding hypointense foci involving the left parietal region, in keeping with acute infarcts. ADC, apparent diffusion coefficient; DWI, diffusion-weighted imaging.",yes
PMC10643080,Figure_5,oa_package/6b/32/PMC10643080.tar.gz,['\nPrenatal ultrasonic imaging of the congenital hemangioma of the affected twin fetus in Case 3 at 33+0 wk of gestation.'],"Figure 5 A: Two-dimensional ultrasound of the abdominal horizontal cross-section of the mass of the affected fetus revealing that the mass was enlarged and heterogeneous; B: CDFI of an abdominal horizontal cross-section of the mass showing no blood flow signal within the mass. M: Mass; R: Right; white arrow, the mass; C: A two-dimensional ultrasound image of the fetal head showing subcutaneous edema (blue arrow); D: A two-dimensional transverse section of the fetal chest showing intrapericardial fluid collection (yellow arrow). H, heart; M, mass; E: A two-dimensional thoracic horizontal cross-section of the affected fetus showing fetal pleural effusion (orange arrow). L: Lung; M: Mass; R: Right; white arrow, the mass; F: Two amniotic cavities on two-dimensional ultrasound showing that the amniotic fluid of the affected fetus (R-AF) was not clear. R-AF: Right amniotic fluid. LAF left amniotic fluid.",yes
PMC7011484,Figure_7,oa_package/49/40/PMC7011484.tar.gz,"['Histologic sections revealed a densely cellular spindled cell neoplasm largely arranged in short fascicles and sheets, permeating native bone trabeculae ().', '006""/>Case 2: core needle biopsy with permeation of spindle cells around bony trabeculae (a).']","Figure 7 Case 2: core needle biopsy with permeation of spindle cells around bony trabeculae (a). Hemangiopericytoma-like vasculature was identifiable (b). The tumor was monophasic, comprised of uniform, ovoid spindle cells with scanty cytoplasm arranged in short fascicles (c, d).",yes
PMC10416515,Figure_1,oa_package/f9/be/PMC10416515.tar.gz,"[' 1 and 2).', ' 1 and 2).', 'Brain magnetic resonance imaging (MRI) performed at A 4 years, B 4 years and 5 months, C 5 years and 2 months, and D 7 years and 6 months of age', 'tif"">Additional file 1: A-F.']","Fig. 1 Brain magnetic resonance imaging (MRI) performed at 4years, 4years and 5months, 5years and 2months, and 7years and 6months of age",yes
PMC9000983,Figure_3,oa_package/af/2e/PMC9000983.tar.gz,"['However, pneumothorax formation conditioned lung atelectasis, hindering visibility and approach to the second lesion; hence, pleural drainage was performed ().', '\nCone-beam computed tomography demonstrating mild to moderate pneumothorax after microcoil positioning.']","Figure 3 Cone-beam computed tomography demonstrating mild to moderate pneumothorax after microcoil positioning. Pneumothorax had to be drained, once the second lesion localization was impaired by atelectasis",yes
PMC4528349,Figure_2,oa_package/e1/83/PMC4528349.tar.gz,"[' 2).', 'Biopsy specimen positive to Calretinin (c) y thrombomodulin (d), supporting the diagnosis of mesotheliomaTwenty four hours after surgery, her AM serum cortisol was measured (20.']","Fig. 2 Immunohistochemistry. A biopsy specimen showing a papillary pattern of the tumor and the absence of mitosis. ( )There is diffuse positivity for ACTH. Biopsy specimen positive to Calretinin ( ) y thrombomodulin ( ), supporting the diagnosis of mesothelioma",yes
PMC11402662,Figure_2,oa_package/fe/f2/PMC11402662.tar.gz,"['At 21 days post BLM administration, the degree of pulmonary fibrosis was evaluated by hydroxyproline quantification (\nSupplementary C\n).', 'However, hydroxyproline levels were comparable between control Tff1\nfl/fl mice and Foxp3-Cre/Tff1\nfl/fl mice (\nSupplementary C\n).', 'Similarly, there was no obvious difference in histopathologic inflammation and collagen deposition between the two groups (\nSupplementary D\n).']",Figure2 Generation of Foxp3-Cre/Tff1-Flp/VeDTR mice. The genome editing strategy of Foxp3 and Tff1-dependent intersectional expression of YFP and DTR. Foxp3-Cre/Tff1-Flp/VeDTR mice were i.t. administered with BLM. Frequency of YFP in lung CD4 T cells was measured over time. Representative plot of day 0 (nave) and day 14 post BLM administration. Total results in indicated time points (n = 6 each). Foxp3-Cre/Tff1-Flp/VeDTR mice were i.t. administered with PBS or BLM (n = 3 each). Frequency of YFP in CD4 T cells of indicated tissues was determined on d21. Frequency of YFP in lung CD4 T cells was measured at indicated time points (n = 6 each). Saline or FTY720 (4 g) or daily i.p. administered in BLM-treated mice from day 0 (n=5 each). Frequency of YFP in CD4 T cells of indicated tissues was determined on d14 Data means with SD. significance assessment: unpaired two-tailed Students t-test. ***; p<0.001.,yes
PMC11316943,Figure_3,oa_package/cc/81/PMC11316943.tar.gz,"['TEE showed significant left-to-right shunt through the iatrogenic atrial septal defect, which was subsequently closed with a 28 32 mm Amplatzer septal occluder device (Abbott Vascular, Santa Clara) (A C).', '.']","Fig. 3. Transcatheter mitral valve-in-valve replacement procedure demonstrating crossing of prosthetic mitral valve ( ), balloon mitral valvuloplasty ( ) and final transcatheter valve in-situ with subsequent iatrogenic atrial septal defect closure with Amplatz occluder ( ).",yes
PMC11287122,Figure_2,oa_package/7d/50/PMC11287122.tar.gz,[],"Fig. 2. RNAseq analysis of lung mRNAs in SARS-CoV-2 infected hACE2 versus hACE2-mCAT mice revealed that mCAT dampened virus induced metabolic reprogramming and innate immune activation. Analysis of RNA transcripts from mouse lung assessed in five SARS-CoV-2 hACE2-infected mice, five hACE2-mCAT infected mice, three uninfected hACE2 mice, and three uninfected hACE2-mCAT mice collected at 4 DPI. Bar plots for statistically significant changes (FDR < 0.14). ( ) Effect of mCAT expression showing striking reversal of viral OXPHOS inhibition ( ) together with partial reversal of viral inhibition of TCA and peroxisomal gene suppression, determined by GSEA for infected versus uninfected lung samples, ranked by nominal enrichment score (NES) ( ). ( ) Heat map representation of mCAT effects on SARS-CoV-2 infected hACE2 and hACE2-mCAT mice on lung HIF and glycolytic mRNA levels, log-2 fold (L2FC). ( ) Effect of mCAT expression on lung innate immune gene expression. ( ) Gene ontology (GO) pathway analysis of RNA transcripts demonstrating the striking reversal of viral innate immune gene induction by mCAT expression. ( ) Volcano plot of RNA transcript levels of hACE2-infected versus uninfected lung samples. ( ) Volcano plot of RNA transcript levels of hACE2-mCAT infected versus uninfected lung samples. ( ) Heat map representation of mCAT effects on SARS-CoV-2 infected hACE2 and hACE2-mCAT mice on innate immune and integrated stress response (ISR) mRNA levels, L2FC. ( ) Heat map representations of mCAT effects SARS-CoV-2 infected hACE2 and hACE2-mCAT mice on selected mitochondrial, NF-B, and interferon mRNA levels, L2FC. ( ) GO pathway analysis of RNA transcripts demonstrating the striking mCAT inhibition of cell death pathways. An O on a line indicates that the difference between with versus without treatment did not change sufficiently to reach FDR < 0.25 or log10(adj- value) >1.3.",yes
PMC3075253,Figure_7,oa_package/9a/c6/PMC3075253.tar.gz,"['g006""/>\n shows D2-40 immune staining of lymphatic endothelial cells revealing arborizing vascular structures and lymphocytic cell infiltration.', 'g007D2-40 staining of arborizing vascular structures; irregular lymphatic vessels.']",10.1371/journal.pone.0018397.g007,yes
PMC6819764,Figure_7,oa_package/88/a3/PMC6819764.tar.gz,[],Supplementary Figure5a,yes
PMC10438012,Figure_1,oa_package/36/f3/PMC10438012.tar.gz,"['The topology and morphology of AstV infected cells (A 1H), as well as confirmatory double immunofluorescent staining for AstV capsid protein and microtubule associated protein 2 (MAP2; pan-neuronal somatodendritic marker) (J and 1K), unambiguously demonstrated that HAstV-NIH infects exclusively neurons.', ', pontine nuclei and medullary reticular formation) (A 1I).', 'Notably, the areas containing infected neurons also displayed a general paucity in MAP2 immunoreactivity, and somatodendritic compartments that harbored AstV capsid protein accumulations were depleted of MAP2 (J) (compared to virus negative somatodendritic compartments, K), suggesting disruption of integrity of the neuronal somatodendritic compartments.', ', putamen; B), thalamus (D), deep cerebellar nuclei (E), Purkinje cells in the cerebellar cortex (F), pontine nuclei (G and 1J), and medullary reticular formation (H and 1K).', 'g001HAstV-NIH infects neurons with the highest burden in the cerebellum and brainstem.', 'g004"" position=""float""/>Since we observed depletion of MAP2 in the somatodendritic profiles that harbored AstV capsid protein by a double fluorescent immunostaining (J and 1K) and our functional genomic analysis also revealed downregulation of genes associated with the neuronal somatodendritic compartments (Prohibitin is a target of miR-128.']","Figure 5 Prohibitin is a target of miR-128. ( ) Prohibitin is a potential target of miR-128. miR-128 targeting site in the 3UTR of prohibitin. ( ) Prohibitin levels were reduced upon treatment with hydrogen peroxide. Cardiomyocytes were treated with 100M hydrogen peroxide, and harvested at the indicated time for the detection of prohibitin by immunoblotting. The total amount of -actin served as internal control. ( ) miR-128 levels were increased upon treatment with hydrogen peroxide. Cardiomyocytes were treated with 100M hydrogen peroxide, and harvested at the indicated time for the detection of miR-128 by qRT-PCR. The results were normalized to that of U6. *p<0.05, vs control. ( ) miR-128 inhibits the translational activity of prohibitin-3UTR. HEK293 cells were infected with adenoviral constructs of miR-128 or -galactosidase (-gal). 24h after infection, cells were transfected with the prohinitin-3UTR luciferase construct. ( ) Enforced expression of miR-128 reduces endogenous prohibitin levels. Cardiomyocytes were infected with adenoviral constructs of miR-128 or -galactosidase (-gal), and harvested 48h after infection for the detection of prohibitin by immunoblotting. The total amount of -actin served as internal control. ( ) Knockdown of miR-128 by its antagomir. Cardiomyocytes were transfected with miR-128 antagomir or the antagomir control, and then treated with 100M hydrogen peroxide. 3h after hydrogen peroxide treatment miR-128 was analyzed by qRT-PCR. *p<0.05, vs control. ( ) Knockdown of miR-128 attenuates the reduction of prohibitin levels. Cardiomyocytes were treated as described for ( ). Prohibitin was analyzed by immunoblotting and the total amount of -actin served as internal control.",yes
PMC9576773,Figure_1,oa_package/c8/8d/PMC9576773.tar.gz,"[' 1A).', ' 1B).', ' 1B).', ' 1C).', ' 1D).', 'CSF GAP-43 according to clinical diagnosis and tau status.', 'CSF GAP-43 was correlated with high CSF p-tau (r = 0.']","Figure 1 CSF GAP-43 according to clinical diagnosis and tau status. ( ) Comparison of CSF GAP-43 between cognitively normal (CN), individuals with mild cognitive impairment (MCI) and Alzheimers disease (AD) dementia. ( ) CSF GAP-43 in individuals according to A/tau/neurodegeneration (ATN) profile. A represents amyloid pathology, T represents tau pathology, and N represents neurodegeneration, the cutoff values (CSF A42<977pg/mL, CSF p-tau>27pg/mL, CSF t-tau>300pg/mL) were used to define the positivity of A/T/N status respectively. ( ) CSF GAP-43 in CN, and in individuals with MCI and AD Dementia, stratified by tau positive status (CSF p-tau>27pg/mL) ( ) comparison of CSF GAP-43 between tau negative (T) and tau positive (T+) status.",yes
PMC5687149,Figure_1,oa_package/90/2c/PMC5687149.tar.gz,"['Although the exact mechanism of AMD pathogenesis is not known, oxidative stress, protein aggregation, and inflammation as well as some genetic factors play a central role in AMD development () [5].', '0-8487733261823422284The exact mechanism of AMD pathogenesis is not known, but several factors can be implicated with a distinct role of aging.']","Figure 1 The exact mechanism of AMD pathogenesis is not known, but several factors can be implicated with a distinct role of aging. Besides aging, various oxidative stress-related environmental and lifestyle influences can be involved. The complement gene mutations play a major role in AMD. Oxidative stress and presumably other factors lead to accumulation of heterogenous lysosomal lipofuscin in retinal pigment epithelium (RPE), which induces a proinflammatory response. This, in turn, can lead to accumulation of extracellular drusen. Lipofuscin contains proangiogenic factors, such as A2E, that may develop choroidal neovascularization typical for wet AMD.",yes
PMC11312878,Figure_12,oa_package/04/16/PMC11312878.tar.gz,[],,yes
PMC6015995,Figure_1,oa_package/ed/28/PMC6015995.tar.gz,"[""Endoscopic image of the patient's 2.""]","Figure 1 Endoscopic image of the patient's 2.4 cm GIST in the fundus of the stomach, shown with circle. GIST -Gastrointestinal stromal tumor",yes
PMC8049071,Figure_1,oa_package/88/12/PMC8049071.tar.gz,[],"FIGURE 1 PropionylCoA carboxylase deficient cells have defective mitochondrial respiration. A, Schematic representation of propionylCoA metabolism and its accumulation in propionylCoA carboxylase (PCC) deficient cells and PCC activity in PCC deficient cells. B, Mitochondrial respiration in PCC deficient and control fibroblasts using consecutive injections of oligomycin, FCCP and Rotenone with Antimycin A (AA). C, Schematic representation of the mitochondrial respiration analysis in permeabilised cells. Digitonin was injected together with ADP and complex specific substrates. D, OCR in permeabilised PCC deficient and control fibroblasts with complex I specific substrates, pyruvate and malate. E, Bar graph of complex I linked respiration in PCC deficient and control fibroblasts in the (meanSD, * indicates <.05)",yes
PMC10643278,Figure_4,oa_package/17/d0/PMC10643278.tar.gz,"['2Case 2A 58-year-old Chinese woman underwent a chest CT as part of a health check-up, and the scan revealed a subpleural lesion in the posterior basal segment of the left inferior lung lobe, with a diameter of approximately 4 cm (: A, B, C, D, arrows).', '(A D) Contrast-enhanced computed tomography revealing a subpleural lesion in the posterior basal segment of the left lower lobe, with a diameter of approximately 4 cm (red arrow).', ')3DiscussionBCs are extremely rare benign masses that are more commonly found in women [6].']","Fig. 4 (AD) Contrast-enhanced computed tomography revealing a subpleural lesion in the posterior basal segment of the left lower lobe, with a diameter of approximately 4 cm (red arrow). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)",yes
PMC4922201,Figure_2,oa_package/a7/72/PMC4922201.tar.gz,"['The cystic spaces, which have resembled vascular spaces, filled with homogenous pink fluid and lymphoid aggregates were evident between cystic spaces ().', '.']","Figure 2. Histologic Micrograph Dilated cystic spaces with flattened endothelial cell lining (black arrow) and filled by homogenous pink fluid. Dilated lymphatic channels of varying sizes, separated by thin septa within the pancreatic parenchyma have shown. Note the lymphoid has aggregated around the lymphatic channels.",yes
PMC5534204,Figure_4,oa_package/e9/a3/PMC5534204.tar.gz,"[' 4a, b).', ' 4c, d); and contractions induced by 10, 30, and 100 M histamine were significantly increased in coronary arteries from MetS pigs as compared to those from Lean and MetS-SN pigs (for 10 M Lean: 1.', ' 4d) were not significantly different among the tested groups.', '', 'The two-way ANOVA test followed by the Student Newman Keuls post hoc test\nWe also employed front-surface fluorometry to assess intracellular Ca2+ ([Ca2+]i) changes in response to KCl and histamine in fura-2 loaded rings (', ' 4) as well as TRPC1 expression and atherosclerosis associations (', ' 4 and 7; and b TRPC1 expression and percentage wall coverage from Figs.']","Fig.4 Isometric tension measurements. , Sample traces of active tension changes induced by KCl or histamine in coronary artery rings from the Lean group are shown. Contractions were induced by cumulatively increasing concentrations of KCl (1070mM, ) or histamine (10 to 10 M, ). , Concentrationresponse curves for KCl and histamine. histamine. The mean active tension value for each data point was calculated by averaging the mean active tension values obtained from 2 to 4 rings in each pig. * <0.05; *** <0.001; for MetS vs. Lean and <0.05; <0.01 for MetS vs. MetS-SN. The two-way ANOVA test followed by the StudentNewmanKeuls post hoc test",yes
PMC9594110,Figure_5,oa_package/13/e2/PMC9594110.tar.gz,"['As illustrated in 5, distribution of the vector genome was homogeneous throughout the 97 123 brain samples analyzed per animal.', ' 5Vector and enzyme activity biodistribution in NHP brainRepresentative NHP injected with 4.']","Figure4 Filipin staining of storage material in the GM1 gangliosidosis cat CNS at 1month Filipin staining appears as punctate white or gray dots in gray matter of untreated GM1 gangliosidosis cats, with little staining in gray matter of wild-type (WT) cats. Filipin staining was apparent in the cerebrum (block D located in A) of all AAV-treated cats, with moderately diminished staining in the cat treated by cisterna magna (ICM) injection. Cerebellar gray matter and brainstem (block H located in A) exhibited profound clearance of storage material after cisterna magna injection (with some variability between cats), but little clearance after bilateral intracerebroventricular (ICV) or intrathecal lumbar (ITL) infusions. Filipin staining was reduced in the lumbar intumescence of the spinal cord (block P located in A) of all treated cats.",yes
PMC8203161,Figure_2,oa_package/e4/e4/PMC8203161.tar.gz,"['6%) (), type 3 (n = 22, 18.', 'Ipsilateral oblique view of the right IIA angiography showing the type 4 anatomy of the PA arising from the internal pudental artery.']",Figure 2 Ipsilateral oblique view of the right IIA angiography showing the type 4 anatomy of the PA arising from the internal pudental artery.,yes
PMC11107136,Figure_2,oa_package/b9/ef/PMC11107136.tar.gz,['Histopathology of cecal mass biopsy (hematoxylin and eosin stained) showing infiltration of lymphocytes (green arrow) and epithelioid cells (yellow arrow) with the formation of a focus of poorly formed granuloma.'],Figure 2 Histopathology of cecal mass biopsy (hematoxylin and eosin stained) showing infiltration of lymphocytes (green arrow) and epithelioid cells (yellow arrow) with the formation of a focus of poorly formed granuloma.,yes
PMC6580084,Figure_3,oa_package/f4/6d/PMC6580084.tar.gz,"['3 AI Thickened, dilated bronchioles located in right lower lobe, false positive case by AI.']","3 AI Thickened, dilated bronchioles located in right lower lobe, false positive case by AI.",yes
PMC6948954,Figure_9,oa_package/e2/84/PMC6948954.tar.gz,[],"Figure 2. Isolated lymphatic endothelial cell clusters in the mouse embryonic kidney that form in response to VEGF-C. ( ) In the developing kidney, PROX1 /LYVE-1 lymphatic endothelial cells appear as either vessels or cell clusters. A representative image from analysis of four kidneys from three independent litters. Lymphatic clusters (white arrowheads) at E16.5 are structurally and anatomically distinguishable from the lymphatic plexus (white asterisk). The segmentation of PROX1 /LYVE-1 lymphatics makes clusters easier to visualize. Scalebar: 50 m. H, hilum; C, cortex. ( ) Confirmation of the lymphatic identity of clusters. Representative confocal -stacks from a minimum of 3 kidneys from three independent litters stained for PROX1, LYVE-1, podoplanin and VEGFR-3 identifies lymphatic clusters (white arrowhead). Filopodia-like projections (white asterisks) extend from the clusters. The relationship of clusters to other structures in the kidney is also shown. Scalebars: 20 m. BE, blood endothelium; G, glomerulus; M, macrophage. ( ) Quantitative assessment of lymphatic cluster dynamics during mouse kidney development. For each embryonic kidney immunolabelled for PROX1 and LYVE-1, the number of clusters (top graph) or the total number of cells within all clusters (bottom graph) in any single kidney was quantified. Each data point represents the values for one kidney and error bars represent standard error of the mean. ANOVA showed a significant overall increase in the total number of clusters per kidney ( 10.9; p 0.0002) and the total number of cells constituting all clusters per kidney ( 15.9; p 0.0001). Brackets between timepoints demonstrate significance when multiple comparisons were performed using Bonferroni tests. *: p 0.0332; **: p<0.0021; ***: p<0.0002, ****: p<0.0001. Raw data and results of all multiple comparisons are presented in . ( ) Volume renderings of a representative E15.5 kidney conveys the heterogeneity of PROX1 /LYVE-1 cluster size and morphology. Scalebar: 5 m. ( ) VEGF-C increases the abundance of lymphatic clusters ex vivo. Representative 3D reconstructions of mouse embryonic kidneys, harvested at E14.5, cultured at the air-liquid interface for 48 hr with or without 40 ng/ml recombinant VEGF-C and immunolabelled for PROX1 and LYVE-1. PROX1 /LYVE-1 lymphatic cell clusters (white arrowheads) were detectable in both treated and untreated explants. The number of PROX1 /LYVE-1 lymphatic cell clusters per kidney was quantified. Each data point represents the values for one cultured kidney and error bars represent standard error of the mean. An unpaired Students test showed that treatment with VEGF-C significantly increased the abundance of PROX1 /LYVE-1 cell clusters within the explants ( 2.77; p=0.0184). Data were pooled from two independent culture experiments. Raw data and results of statistical tests are presented in .",yes
PMC11027919,Figure_5,oa_package/a9/d1/PMC11027919.tar.gz,[],Figure 4 Chest tomography showing alveolar-interstitial opacities in the left lower lobe.,yes
PMC9465245,Figure_4,oa_package/c2/4c/PMC9465245.tar.gz,"['04) ().', 'These results correlate with a decreased trend in total reticulocyte count in the CBC in the hypersplenism group ().']","FIGURE 4 RBC properties of SCD patients Asplenic/hyposplenic compared to Hypersplenic using Flow cytometry: + FSC and SSC of RBC; Annexin V positive RBC; fluo-4 high-positive RBC; MBBR high-positive RBC; CD71 positive RBC. Group (A) patients with asplenia/hyposplenism, Group (B) patients with hypersplenism.",yes
PMC3394088,Figure_1,oa_package/98/86/PMC3394088.tar.gz,"['These data feature a broad range of cell/nucleus morphologies ranging from normal gastric mucosa (regular round/oval shaped nuclei) to bone marrow with primary myelofibrosis (highly irregular neoplastic megacaryocytes, d and ', 'H E stained tissue samples: (a) breast cancer; (b) liver; (c) gastric mucosa; (d) bone marrow with primary myelofibrosis with highly pleomorphic megakaryocytes (without cell cluster separation); (e) connective tissue; (f) kidney tissue; (e, f) examples of the method validation (dots represent manually-assigned labels, green: labels classified as true positive, red: false negatives, yellow: false positives).']","Figure 1 H&E stained tissue samples: (a) breast cancer; (b) liver; (c) gastric mucosa; (d) bone marrow with primary myelofibrosis with highly pleomorphic megakaryocytes (without cell cluster separation); (e) connective tissue; (f) kidney tissue; (e, f) examples of the method validation (dots represent manually-assigned labels, green: labels classified as true positive, red: false negatives, yellow: false positives).",yes
PMC8666636,Figure_5,oa_package/83/be/PMC8666636.tar.gz,['4DiscussionIt is the first case of non-pancreatic pseudocyst found in soft tissue of oral and maxillofacial region.'],"Fig. 5 40x view, sections from a cyst shows a tissue with no definitive epithelial lining.",yes
PMC9723465,Figure_1,oa_package/8d/3a/PMC9723465.tar.gz,[],"Figure1 Examples of image patches included in the dataset. Squamous cell carcinoma , basal cell carcinoma , melanoma , naevi , epidermis , chrondral tissue , dermis , nerves , necrosis , skeletal muscle , hair follicles , sweat glands/eccrine glands , sebaceous glands , vessels , subcutis and elastosis . All images are 100 x 100 m (395 x 395 px) in size, scale bar = 20 m.",yes
PMC7689516,Figure_11,oa_package/73/94/PMC7689516.tar.gz,[],Figure11 Mode of transformation of medical images and Segmentation procedure.,yes
PMC7390889,Figure_2,oa_package/59/b8/PMC7390889.tar.gz,"['This procedure resulted in 5 components located in 5 networks, default-mode (DMN), dorsal attention (DAN), auditory (AUD), sensorimotor (SMN), and visual (VN), shown in .', 'Identified networks.']","Figure 2 Identified networks. Based on the anatomical and functional properties, five components were located in five networks (DMN, DAN, AUD, SMN, VN). Brighter parts indicate stronger local activity, and under each part is its color bar and peak coordinates.",yes
PMC6532616,Figure_1,oa_package/ad/5d/PMC6532616.tar.gz,"['We classified the findings of ulnar nerve dislocation into three categories; non-dislocation, partial dislocation and complete dislocation ().', '6972/fig-1The ultrasonographic classifications of ulnar nerve (dotted line) displacement during elbow flexion.']",10.7717/peerj.6972/fig-1,yes
PMC4926090,Figure_2,oa_package/fa/72/PMC4926090.tar.gz,"['Interestingly, ApoE / /OPN / mice showed markedly reduced glomerular lipid deposition compared with ApoE / mice despite consumption of HD (ApoE / /OPN / HD mice) (A,B).', '001) (C).', 'org/1999/xlink"" xlink:href=""srep28882-f1""/>Renal lipid accumulation in the four groups after 4weeks of dietary treatment.']","Figure 2 Renal lipid accumulation in the four groups after 4weeks of dietary treatment. ( ) Representative oil red O staining in the kidneys. Red: oil red O-positive cells; blue: hematoxylin counterstaining. Scale bar=50m. ( ) Representative oil Red O staining in the kidneys. Red: oil red O-positive cells; blue: hematoxylin counterstaining. Scale bar=500 m. ( ) Bar graph showing oil red O-positive cells in the kidney. Data are meansSEM; n=4 in each group. *P<0.001. Two-way ANOVA, gene, p<0.001; diet, p<0.001; genediet, p<0.001.",yes
PMC4977832,Figure_7,oa_package/83/10/PMC4977832.tar.gz,"[' 7).', ' 7).', ' 7), because it is specific for human S (Additional file 1: S1).', ' 7).', 'Scale bar = 50 mFor our human autopsy cohort, we performed IHC on sections of midbrain (PD and DLB), cingulate cortex (DLB; ']","Fig. 7 Comparison of pSer129 antibody IHC staining using nave S transgenic and WT mice. Representative images of IHC staining of brainstem tissue from a 7month old non-symptomatic homozygous M83 mouse (M83 unimpaired), a 12month old motor impaired homozygous M83 mouse (M83 motor impaired) and a WT mouse. All antibodies stained perikaryal and neuritic inclusions in the M83 diseased mouse. LS7 showed weaker reactivity to S pathology than the other antibodies and showed stronger diffused signal in the S transgenic mice compared to the WT mouse. LS3-2C2 also showed weak staining of pathology. In addition to the pathology in motor impaired M83 mouse, antibodies 4F8, LS3-2C2, LS4-2C3 and 81A also labeled neuronal projections even in WT mice (arrowheads). EP1536Y exhibited some nuclear staining in some mouse sections (arrows), as did LS3-2C2, but much weaker than EP1536Y (eg. see arrows in the M83 unimpaired mouse). Scale bar=50m",yes
PMC5545859,Figure_1,oa_package/20/84/PMC5545859.tar.gz,"['1).', 'T2-weighted MRI with right temporal arachnoid cyst with signs of intracystic hemorrhage (a).', 'Initial intraoperative endoscopy showed a thick, yellowish lining of the inner wall of the cyst, (c) which was removed under endoscopy (d) with eventual total resection (e)\nHematoxylin-eosin-stained sections (']","Fig. 1 T2-weighted MRI with right temporal arachnoid cyst with signs of intracystic hemorrhage ( ). Postoperative control-MRI shows total resection of the tumoral tissue within the cyst ( ). Initial intraoperative endoscopy showed a thick, yellowish lining of the inner wall of the cyst, ( ) which was removed under endoscopy ( ) with eventual total resection ( )",yes
PMC11598987,Figure_3,oa_package/1e/23/PMC11598987.tar.gz,"['Age DistributionThe age distribution of the cases () showed a progressive increase in tumor frequency with age.', 'Number and distribution of tumor cases by sex, reproductive status and age class.']","Figure 3 Number and distribution of tumor cases by sex, reproductive status and age class. ( ) Female dogs; ( ) male dogs; ( ) female cats; ( ) male cats.",yes
PMC6539425,Figure_1,oa_package/07/9c/PMC6539425.tar.gz,"['1 to 4 mg/kg resulted in a dose-dependent reduction in circulating C5 levels at day 9 after injection ( 1A).', 'administration of ALN-CC5 was highly durable, with animals treated with the 3 mg/kg dose achieving C5 silencing of greater than 85% by day 7 and 50% C5 silencing still observed by day 70 ( 1B).', ' 1ALN-CC5 Lowers Circulating C5 Protein Levels and Complement Hemolytic Activity in Mice and Rats(A and B) Levels of mouse serum C5 protein were measured by ELISA.', '5 to 25 mg/kg resulted in a dose-dependent reduction in rat liver C5 mRNA levels, with up to 90% reduction at the peak dose on day 8 ( 1C).', 'Classical pathway hemolytic activity showed a dose-dependent reduction of up to 75% at the top dose ( 1D).', 'Overall, the level of hemolysis suppression achieved in cynomolgus monkeys was comparable to or greater than that observed in rats ( 1D).', 'Hemolytic activity reduction in the context of the inflammatory EAMG model (induced with complete Freund s adjuvant) was comparable to that achieved in healthy rats ( 1C).']","Figure1 ALN-CC5 Lowers Circulating C5 Protein Levels and Complement Hemolytic Activity in Mice and Rats (A and B) Levels of mouse serum C5 protein were measured by ELISA. (A) ALN-CC5 potency at day 9 after single-dose treatment. (B) Duration of C5 reduction after single-dose treatment. N= 5 per group. Dotted line is the assay background observed in C5-deficient DBA/2 mice. (C and D) Female rats were evaluated on day 8 following a single injection of 2.5, 5, 10, or 25mg/kg of ALN-CC5. (C) C5 liver mRNA was quantified by qRT-PCR and normalized to untreated rats. (D) Serum hemolytic complement activity was quantified using a sensitized sheep red blood cell (RBC) lysis assay. N= 34 animals per group; error bars are SD. The 5mg/kg group showed significant reduction at p< 0.05, and the 10 and 25mg/kg groups showed significant reduction at Bonferroni-corrected p< 0.0125. Error bars are SD.",yes
PMC5042456,Figure_1,oa_package/61/12/PMC5042456.tar.gz,"['Placental neutral storage lipidsMolecular speciation of the neutral storage lipids CE and TAG was compared between groups ().', 'Thirteen CE species were quantified, with ester-linked carbon chain of lengths from C14 to C22, shown in A.', 'g001Storage Lipid Profiles in Placenta.', 'g001""/>A total of 22 TAG lipid species are reported, shown in B and 1C.']",10.1371/journal.pone.0163972.g001,yes
PMC4791226,Figure_6,oa_package/1a/23/PMC4791226.tar.gz,"['PGC-1 overexpression prevents cachexia in LLC-bearing miceGastrocnemius (GSN), tibialis (TIB) and heart weight A.']","Figure 6 PGC-1 overexpression prevents cachexia in LLC-bearing mice Gastrocnemius (GSN), tibialis (TIB) and heart weight and tibialis fiber CSA in female control WT (C WT), PGC (C PGC) and LLC-bearing mice WT (LLC WT) and PGC (LLC PGC). Data (meanSD) are expressed as percentages of C WT. Gene expression analysis of Atrogin-1 and MuRF1 transcripts. Specific mRNA abundance was corrected for the mean of TBP (TATA box-binding protein) and -actin levels on individual samples. Data (means SE) are compared by 2-way ANOVA. Significance of the differences: * < 0,05 C WT, ** < 0,01 C WT, *** < 0,001 C WT, $ < 0,05 LLC WT, # < 0,05 C PGC, ## < 0,01 C PGC.",yes
PMC8235091,Figure_5,oa_package/e8/ba/PMC8235091.tar.gz,"['(vii) Laser-assisted removal of cancerous changes/tissues (papilloma, polyps, haemangiomas, vocal nodules) enables precise operation and reaching narrow channels in nasal, sinus and other regions see .', 'Laser-assisted removal of cancerous tissue.']",Figure 5 Laser-assisted removal of cancerous tissue.,yes
PMC9012573,Figure_3,oa_package/e2/5c/PMC9012573.tar.gz,[],"Figure 3 Basal cell carcinoma pulmonary metastasis 40x, 100x, and 200x magnification view (from left to right) with H&E staining",yes
PMC5467336,Figure_3,oa_package/5f/3a/PMC5467336.tar.gz,"['002""/>SORL1 binding to APP is decreased in E4/E4 neurons.']","Figure 3 SORL1 binding to APP is decreased in E4/E4 neurons. (a) Protein samples from control (C) and E4/E4 (E4) neurons, after five weeks in culture, were immunoprecipitated with mouse anti-SORL1 antibody (co-IP SORL1) and analyzed with rabbit anti-APP. TL, total lysate; Co-IP, immunoprecipitate. (b) WB analysis of control (C) and E4/E4 (E4) neurons after five weeks in culture (Ctrl, untreated neurons) with vehicle (V, DMSO 0.01%) (b-inh, 250 M), and (g-inh, 50nM) secretase inhibitors. (c) The same samples were immunoprecipitated with anti-APP antibody and analyzed with anti-SORL1 by WB. Densitometric analysis (OD) is reported in (d) = 4; one-way ANOVA with post hoc Tukey's test. < 0.05 versus untreated E4/E4 neurons. (e) To counteract A 42 production, neurons were exposed to (b-inh, 250nM), (g-inh, 50nM), and + (b+g-inh) secretase inhibitors after four weeks of plating (blue histogram), and A 42 levels were assessed one week later, five weeks (red histograms) by ELISA. (f) Confocal microscopy analysis of E4/E4 neurons incubated with or without secretase inhibitors and stained with an anti-A antibody. Nuclei are marked in blue. Note that the anti-A antibody also detects full length APP. Pictures are representative of four experiments ( = 4) performed in triplicate. Scale bar: 7 m. Media with or without inhibitors were refreshed every two days. The level of A 42 (expressed as the ratio of A 42/40) and the extent of intact NeuN-positive nuclei (survival) are reported in (e). Each data point is the meanSEM of triplicate determinations of three independent experiments ( = 3) and is expressed as the percentage of values from E4/E4 neurons after 4 weeks of plating.",yes
PMC4297388,Figure_2,oa_package/36/12/PMC4297388.tar.gz,"['The mammary glands of control mice (MMTV-Cre; Sox9wt/wt) showed that Cre expression is indeed mosaic, with expression in ~50% of epithelial cells throughout development ( 2).', 'Importantly, the proportion of Cre-expressing epithelial cells in the Sox9 cKO mammary glands was markedly diminished ( 2).', 'However, the proportion of Cre- expressing mammary epithelial cells is further diminished by 5 weeks, and we were unable to find any such cells in 8-week old Sox9 cKO mice, while such cells were abundant in the age matched control mice ( 2).', '\nSox9 deleted cells are lost over time.', 'Unfortunately, direct staining of Sox9 was not possible due to non-specific staining within the mammary gland.', 'As we observed depletion of Cre-expressing cells in both luminal and myoepithelial compartments of 8-week old MMTV-Cre-driven Sox9 conditional KO mice ( 2), we anticipated the lineage-trace to show an absence of Sox9-null progeny in both mammary cell compartments.', 'We hypothesized that recovery from the developmental block imposed by Sox9 deletion is overcome by the expansion of mammary epithelial cells in which Sox9 deletion did not occur, due to the known mosaic expression of MMTV-Cre that we used ( 2).', 'Consequently, we used Cre expression as a surrogate for Sox9 deletion and found that Sox9 deleted cells are lost in cKO mice by 8 weeks ( 2).']","Figure 2 Immunostaining of control (MMTV-Cre; Sox9 ) and Sox9 cKO (MMTV-Cre; Sox9 ) mouse mammary glands cut sections (3, 5, 8weeks old mice) with Cre antibody (red). Green and blue represent anti-SMA and DAPI staining, respectively.",yes
PMC6925794,Figure_1,oa_package/1e/29/PMC6925794.tar.gz,"['Marked varus instability of the left ankle was observed during the stance phase of walking ().', 'There was marked swelling, but no instability ().', 'A skin ulcer had formed at the lateral malleolus ().', '0-003667797012199380Clinical picture.']","Figure 1 Clinical picture. (a) The patient is limping, but not using any assistive device. Marked varus instability of the left ankle is observed during the stance phase of walking. (b) There is marked swelling but no instability of the left knee joint. (c) The left ankle joint has marked swelling, and the foot is medially dislocated. A skin ulcer has formed at the lateral malleolus.",yes
PMC4427622,Figure_9,oa_package/4a/86/PMC4427622.tar.gz,"[' 9).', ' 9a).', ' 9b).', ' 9c; F(3, 66) = 4.', ' 9d), spending more time in the target quadrant, as was seen also with wild-type controls.', '', 'Insets depict representative swim paths for the last acquisition trial on day 4 (top row) and during probe test (bottom) for WT (left) and L66 or L1 (right)\nOver-expression of the truncated PHF-core repeat domain fragment of tau in L1 is characterised by non-fibrillar tau pathology associated with a prominent cognitive phenotype and minimal motor featuresThe truncated repeat domain fragment of tau corresponding to residues 296 391 or its homologues in other tau isoforms is the predominant constituent of the core of the PHFs found in the neurofibrillary tangles of AD.', ' 9).', ' 9e; gene factor: F(1, 68) = 11.', ' 9f; F(1, 63) = 22.', ' 9g; F = 1).', ' 9h; t = 3, p = 0.', ' 9i l).', ' 9j; gene factor: F(1, 60) = 26.', ' 9k; gene factor: F(1, 60) = 11.', ' 9l).']","Fig.9 Spatial learning deficits are absent in L66 transgenic mice but observed in L1. Standard open field water maze acquisition learning and recall are shown for L66. No spatial phenotype was observed in L66 mice aged 4months ( WT =12; L66 =13). There were no differences in path length ( ) and swim speed ( ), but lower levels of thigmotaxis occurred on the first 2days of training ( see for <0.05). Also, there was no deficit in recall indexed as quadrant time ( ). Standard open field water maze acquisition learning and recall are shown for cohorts of L1 mice aged 3months ( WT =9; L1 =10) and 6months ( WT =10; L1 =13). Three-month-old L1 mice need longer swim paths to find the hidden platform ( ), but eventually acquire the spatial task after 24 trials (day 4). Note that there was no difference in path length in trial 1, but a strong retardation in learning. Young L1 mice swam faster throughout acquisition ( ), but thigmotaxis was not significantly enhanced ( ). Recall (time in quadrant) tested 1h post-training was deficient in L1, but there was a weak tendency for the target quadrant in controls ( ). Deficits in 6-month-old L1 mice were more pronounced. Transgenic mice failed to acquire the spatial task ( ), swam considerably faster ( ) and maintained high levels of thigmotaxis ( ) relative to age-matched controls. Consistent with the failure to acquire the task, there was no spatial bias for the target quadrant in L1 subjects ( ) whereas controls developed strong memory for the platform location. All data represent meanSE. indicate * <0.05, ** <0.01, *** <0.001, **** <0.0001 for group comparison; <0.05 relative to chance. depict representative swim paths for the last acquisition trial on day 4 ( ) and during probe test ( ) for WT ( ) and L66 or L1 ( )",yes
PMC5342340,Figure_4,oa_package/dc/5b/PMC5342340.tar.gz,['Inhibition of HSP90 decreased inflammatory and NF- B signaling in PHA.'],"Figure 4 Inhibition of HSP90 decreased inflammatory and NF-B signaling in PH , Analysis of the inflammatory cytokine (CRP, TNF- and IL-1) levels in plasma in rats ( = 6 in MCT group, and = 11 in 17-AAG group). ** < 0.01 control group, and # < 0.05, ## < 0.01 MCT group. , Representative images of immunohistochemistry of p65, phosphorylated p65 and HSP70 in lung tissues lesions ( = 6 in MCT group, and = 11 in 17-AAG group).",yes
PMC10949877,Figure_1,oa_package/4a/d3/PMC10949877.tar.gz,"['\n7\n Molecules of the drug bind to ergosterol molecules in the fungal cell membrane, forming channels that assemble into a transmembrane pore; subsequent leakage of intracellular ions leads to cell death by osmotic lysis ().', '1177_1098612X231220047-img4"" position=""float""/>Synthesis of ergosterol an essential fungal cell membrane component commences with the conversion of squalene to lanosterol by the enzyme squalene epoxidase (1).', '1177_1098612X231220047-fig1"" position=""float""/> Mechanism of action FC is a prodrug that is converted to 5-flurouracil (5-FU) by cytosine deaminase inside the fungal cell.', ' Mechanism of action A synthetic allyl-amine, TRB inhibits squalene epoxidase, blocking conversion of squalene to lanosterol ().', ' This results in inhibition of fungal cell growth, or loss of the osmotic integrity of the fungal cell wall and subsequent cell lysis ().']",,yes
PMC11402349,Figure_3,oa_package/ad/52/PMC11402349.tar.gz,['34\nDiagram displaying bone marrow aspiration.'],Figure 3 Diagram displaying bone marrow aspiration. Fluoroscopic imaging of the posterior iliac crest with the Jamshidi needle. A hammer is used to gently penetrate the cortex of the iliac crest prior to aspiration.,yes
PMC7427099,Figure_2,oa_package/5c/a8/PMC7427099.tar.gz,"[' 2A; Table S2).', ' 2A; Table S2).', ' 2B).', ' 2C).', ' 2D).', ' 2E).', 'Homozygous Snx14 mutation causes embryonic lethality in mice.', 'Snx14 mutations in mice result in placental abnormalitiesTo investigate the potential cause of the embryonic lethality in Snx14KO/KO mice, individual placentas were collected and sectioned for histology.']","Figure 2 Homozygous mutation causes embryonic lethality in mice. ( ) Viable embryos are not detected at Mendelian ratios at E10.5 and no mice were found at P0. ( ) weighed less than their and littermates. Bars=MeanSD, * <0.05, ** <0.01, one-way ANOVA. ( ) embryos appear smaller, without clear vascularisation in the head (insets). ( ) Surface visualisation of and embryos with optical projection tomography. ( ) Internal visualisation of and embryos with high resolution episcopic microscopy (HREM).",yes
PMC10669759,Figure_2,oa_package/45/ca/PMC10669759.tar.gz,"['Usual-Type IDC-PThe vast majority of IDC-P is found in association with GG 2 PCa and is considered to represent a retrograde spread or colonization of benign glands by PCa ().', 'Usual-type IDC-P with GG5 prostate cancer.']","Figure 2 Usual-type IDC-P with GG5 prostate cancer. IDC-P glands show cribriform morphology and are surrounded by invasive cancer glands ( ). IDC-P glands have residual basal cells highlighted by basal cell marker P63 (brown stain), while the cancer glands lack basal cells ( ).",yes
PMC10916964,Figure_3,oa_package/c8/31/PMC10916964.tar.gz,[],"FIGURE 3 Characteristic biochemical pattern of tau species in human retina and brain. The amount of tau in temporal neocortex tissue (Brodmann area 20) was higher compared to retina tissue. Oligomeric and/or phosphorylated tau larger than 55kDa was found in brain samples from AD, PART, and control groups but was not evident in the retina. The ptau antibody was present in both retinas and brains, exhibiting a similar biochemical pattern. A 28kDa ptau fragment of 3R tau in controls and longer ptau fragments in PART and AD were observed in brain samples but were nearly negligible in the retina. The specific bands corresponding to the 3R and 4R tau isoforms were identified in the retina. The concentration of these tau isoforms suggests their presence as aggregates and/or phosphorylated proteins larger than 55kDa, with the highest concentration observed in AD cases. Notably, ptau was present in retinas and brain samples from control subjects, suggesting its importance in physiological phosphorylation processes. ( : AD case nr. 11; PART case nr. 32; Control case nr. 36; : AD case nr. 51; PART case nr. 43; Control case nr. 48). 3R, 3repeat; 4R, 4repeat; AD, Alzheimer's disease; PART, primary agerelated tauopathy; PReT, primary retinal tauopathy; ptau, phosphorylated tau",yes
PMC10976582,Figure_4,oa_package/87/d4/PMC10976582.tar.gz,['Recommended practices for the perclose proglide vascular closure device.'],"Figure 4 Recommended practices for the perclose proglide vascular closure device. The Perclose Proglide platform (Abbott, Chicago, IL) is a suture-mediated vascular closure device commonly used in contemporary practice to hemostase standard and large bore femoral arterial access sites. It consists of two needles in the proximal compartment and a catheter that house the suture. It is 0.035 J wire compatible. This cartoon depiction demonstrates the steps of large bore access and closure using this platform. ( ) Using ultrasound guidance, the common femoral artery is accessed with a 21 gauge micrpuncture needle 1-3cm below the probe. ( ) Once femoral access is obtained, a.018 micro access guidewire should then be advanced under fluoroscopy to ensure appropriate passage into the ipsilateral common iliac artery and abdominal aorta. ( ) Following confirmation of the femoral access site, the arteriotomy may be pre-closed using 1 or 2 Proglide sutures, typically in the 10- and 12-oclock positions. Here, the percutaneous ventricular assist device is secured in place following dilation of the arteriotomy ( ) During deployment of the Proglide system, the catheter is advanced over an 0.035 J wire through the arteriotomy. ( ) Once the return of pulsatile blood is noted from the side arm, the device lever is pulled and two simultaneous needles are deployed through the anterior wall of the femoral artery utilizing the non-biodegradable polypropylene microfilament with preformed knot that is tightened to form the suture loop. The suture is then retracted to the anterior wall of the arteriotomy site and deployed to achieve hemostasis.",yes
PMC4279411,Figure_4,oa_package/a5/63/PMC4279411.tar.gz,"['Western blot analyses of relative expression levels of epithelial-mesenchymal transition (EMT)-related genes in cancer stem cells (CSC) following simultaneously modulation of Oct-4 and Nanog expression and TGF- stimulation in vitroCSC were transfected with mock or Oct-4 and Nanog siRNAs, vehicle, or Oct-4 and Nanog-expressing plasmids for 24 h and stimulated with TGF- for 72 h.']","Figure 4 Western blot analyses of relative expression levels of epithelial-mesenchymal transition (EMT)-related genes in cancer stem cells (CSC) following simultaneously modulation of Oct-4 and Nanog expression and TGF- stimulation CSC were transfected with mock or Oct-4 and Nanog siRNAs, vehicle, or Oct-4 and Nanog-expressing plasmids for 24 h and stimulated with TGF- for 72 h. The relative expression levels of EMT-related genes in the different groups of cells were characterized at the indicated time points post-stimulation by western blot assays. Data shown are representative images (A) or are expressed as the means standard deviation of the relative levels of each protein to the control GAPDH (B) at 72 h post-stimulation from 3 separate experiments. A similar pattern of the relative levels of targeting proteins to the control was detected in the different groups of CSC at 24 h post-stimulation (data not shown). A: Oct-4- and Nanog-silenced CSC; B: TGF--stimulated Oct-4- and Nanog-silenced CSC; C: Oct-4- and Nanog-overexpressing CSC; D: TGF--stimulated Oct-4- and Nanog-overexpressing CSC. *p 0.05 vs. TGF--unstimulated Oct-4- and Nanog-silenced CSC; #p 0.05 vs. TGF--unstimulated Oct-4- and Nanog-over-expressing CSC.",yes
PMC5101594,Figure_4,oa_package/df/de/PMC5101594.tar.gz,['Assessment of Bdkrb1 and Bdkrb2 in the aortas of rat tissues.'],"Figure 4 Immunohistochemistry staining for Bdkrb1 and Bdkrb2 in the aortas of rat tissues. Bdkrb1 protein expression is induced in the aortas of smoking (CS) group. CS-induced expression was abolished in the presence of AO in the (CS + AO). All pictures were taken at 40X magnification. Quantification of the intensity of the immunofluorescence of Bdkrb1. Intensity of Bdkrb1 staining was determined from ZEN software and normalized to the Hoechst level relative to the Control samples. CS group had a significantly higher Bdkrb1 to Hoechst ratio compared to control. Bdkrb1 staining was significantly reduced in CS + AO in comparison with CS. Error bars represent SE. One Way Anova test was used to check for significance between the groups. Asterisks indicate statistically significant associations ( < 0.05). Quantification of the intensity of the immunofluorescence of Bdkrb2. Intensity of Bdkrb2 staining was determined from ZEN software and normalized to the Hoechst level relative to the Control samples. Bdkrb2 staining was significantly reduced in CS + AO in comparison with CS. Error bars represent SE. One Way Anova test was used to check for significance between the groups. Asterisks indicate statistically significant associations ( < 0.05). and : protein expression assessment of the Bdkrb1 and Bdkrb2 in rat aortas. Protein expression of Bdkrb1, but not Bdkrb2, was induced in the aortas of CS group. AO significantly reduced the expression of Bdkrb1 in the CS + AO group. Data on each protein was normalized to -actin. Error bars represent SE. One Way Anova test was used to check for significance between the groups. Asterisks indicate statistically significant associations ( < 0.05).",yes
PMC11119523,Figure_12,oa_package/61/0f/PMC11119523.tar.gz,[],"Figure 12 Stage IIIC1ii. A 48-year-old female patient with high-grade endometrial carcinoma. Sagittal T2 ( ) and T1 fat sat post-contrast ( ) show a large mildly T2 hyperintense hypovascular endometrial mass (arrow) causing expansion of the endometrial cavity with diffusion restriction evidenced by a relatively high signal in DWI ( ) and low ADC value ( ). The mass extends inferiorly through the cervical canal involving the posterior lip and the posterior final fornix. Axial T2 images ( , ) show bilateral iliac metastatic lymph nodes (arrowhead).",yes
PMC5838784,Figure_2,oa_package/b9/9d/PMC5838784.tar.gz,['MMP 3 is expressed in human respiratory epithelial cells in TB patients and MMP 3 gene expression and secretion are driven by IL 17.'],"Figure 2 MMP3 is expressed in human respiratory epithelial cells in TB patients and MMP3 gene expression and secretion are driven by IL17. (A) Pulmonary epithelial cells adjacent to TB granulomas express MMP3. The figure is representative of lung biopsies from five patients with TB (scale bar = 20 m). (B) Minimal staining for MMP3 was observed in five normal lung specimens (scale bar = 20 m). (C) Kinetic experiments in NHBE cells demonstrated that MMP3 secretion increased progressively until 72 h after stimulation with 10 ng/ml IL17 ( < 0.001). (D) IL17driven mRNA accumulation peaked at 24 h, rising to fourfold above baseline ( < 0.01). (E) Direct infection of NHBE cells with Mtb at an MOI from 0.1 to 10 did not alter MMP3 secretion and there was no synergy between Mtb infection and IL17 stimulation.",yes
PMC3249201,Figure_2,oa_package/75/10/PMC3249201.tar.gz,"[' 2) [84].', '', 'The molecules involved have been color-coded, and where red proteins are direct targets of miRNAs, blue proteins depict downstream molecules within the pathways and green proteins are upregulated by miRNAs\nRecently, miR-132 was shown to repress translation of p250GAP, an NMDAR receptor-associated Rho GTPase-activating protein (GAP), which regulates spine morphogenesis by modulating Rac1 and/or RhoA (', ' 2).', ' 2).', ' 2).']","Fig.2 Overview of miRNAs involved in post-synaptic spine growth or shrinkage with several implicated downstream pathways. The indicated miRNAs can modulate the spine morphologies by targeting components of the actin cytoskeleton, regulate protein expression by targeting transcription factors or (indirectly) targeting components of synaptic ion channels. The molecules involved have been color-coded, and where are direct targets of miRNAs, depict downstream molecules within the pathways and are upregulated by miRNAs",yes
PMC4392486,Figure_4,oa_package/95/d1/PMC4392486.tar.gz,"['[3] In children with a pre-natal diagnosis, a CT scan was performed at 1 month, to confirm and precisely locate the malformation [].', '[8]Type I Congenital pulmonary airway malformation.']",Figure 4 Type I Congenital pulmonary airway malformation. (a) Coronal T2-weighted image at 25 weeks of foetal life. Huge multi-cystic mass of the left hemithorax with right mediastinal shift. (b) X-ray at birth with inhomogeneous aeric pattern of the left hemithorax and right shift of the mediastinum. (c and d) transverse and coronal CT-scan images at 4 days of life. Large air-filled cystic areas of the left lower lobe with subtlepneumotorax and normal upper left lobe,yes
PMC6216100,Figure_2,oa_package/e2/b6/PMC6216100.tar.gz,"['Transgene expression was lower in the EDL muscles compared to the TA muscles (s 2B, 2E, and 2F).', '41 Immunofluorescence (IF) analysis revealed that utrophin was localized to the sarcolemma in cross-sections from the heart, diaphragm, EDL, and TA muscles from treated mice compared to vehicle control mice (s 2A 2E).', 'Longitudinal sections from TA muscles also confirmed localization of utrophin to the sarcolemma along the myofibers ( 2F).', ' 2Utrophin Expression in 21-Week-Old D2/mdx Mice Administered AAV- -Utro Compared to Vehicle Control MiceLocalization assessed by immunofluorescence and expression of full-length and micro-utrophin (Utro and -Utro, respectively) in cross-sections from (A) heart, (B) extensor digitorum longus (EDL), (C) diaphragm, (D) soleus, and (E) tibialis anterior (TA) muscles.']","Figure2 Utrophin Expression in 21-Week-Old D2/ Mice Administered AAV--Utro Compared to Vehicle Control Mice Localization assessed by immunofluorescence and expression of full-length and micro-utrophin (Utro and -Utro, respectively) in cross-sections from (A) heart, (B) extensor digitorum longus (EDL), (C) diaphragm, (D) soleus, and (E) tibialis anterior (TA) muscles. Utrophin IF was also assessed in longitudinal section of TA muscles (F). Expression was assessed by western blot and shown relative to loading control (total protein stain; TPS). Scale bars, 100m, n= 8/group.",yes
PMC5671704,Figure_4,oa_package/9e/f5/PMC5671704.tar.gz,"['Thereafter, he began having progressively worsening ICP spikes, at which point repeat head CT was done (), once again showing large accumulation of subdural mass.', 'As seen grossly and as in , the contents of the subdural material were very different from hematoma which was visible on the same cut on the CT scan as a small, residual subtemporal hematoma after the first surgery.', '003""/>Repeat CT 6 hours after first surgery showing reappearance of mixed density mass along the right convexity with again persistent midline shift ((a) and (b)).']","Figure 4 Repeat CT 6 hours after first surgery showing reappearance of mixed density mass along the right convexity with again persistent midline shift ((a) and (b)). There is reherniation with loss of right temporal horn and basilar cisterns once again (c). Of note, the imaging characteristics of the small subtemporal hematoma are unchanged (d), speaking to the differing contents of the panhemispheric subdural density (versus hematoma) seen in panels (a) and (b).",yes
PMC4183529,Figure_6,oa_package/70/31/PMC4183529.tar.gz,"['7 cells, ERK activation was slightly but significantly increased (A) and levels of TNF- , CXCL2 and CCL2 were augmented compared with the control cells (', 'g006Knock down of Spred-2 in RAW264.']",10.1371/journal.pone.0108914.g006,yes
PMC6929096,Figure_2,oa_package/69/9c/PMC6929096.tar.gz,"['Histological scores for all parameters were minimal in the non-DSS treated mice, and there were no notable differences observed with azathioprine treatment in this group ().', 'In contrast, histological analysis of the colon from DSS mice revealed significant increases in scores for inflammation severity (A, p 0.', '05) and extent (B, p 0.', 'It was also observed that the colons from DSS/vehicle mice showed decreased tissue regeneration (as indicated by the higher regeneration score; C, p 0.', '05) and increased crypt damage (D, p 0.', 'No significant differences were observed in tissue regeneration (C) and crypt damage (D) in non-DSS/azathioprine and DSS/azathioprine treated mice, indicative of a partial protection of azathioprine treatment to the colon.', ').', 'Colon pathology of azathioprine and vehicle treated mice treated with 3% DSS.']","Figure 2 Colon pathology of azathioprine and vehicle treated mice treated with 3% DSS. Histological scoring of colons, ( ) Inflammation severity score; ( ) inflammation extent score; ( ) regeneration score; ( ) crypt damage score; ( ) representative Hematoxylin & Eosin -stained sections of colon. Data are presented as mean S.D. ( = 6/group). * < 0.05, ** < 0.01. Scale bar = 100 m.",yes
PMC4856964,Figure_3,oa_package/1e/d8/PMC4856964.tar.gz,"['.', 'Loss of HDAC5 reduces Sin3a-HDAC1/2 interactionsWe asked whether HDAC5 might have a non-catalytic and non-canonical role as a scaffolding molecule, by examining how the interactions of Nkx2.']","Figure 3. Nkx2.5 fails to co-immunoprecipitate HDAC2 and Sin3a in HDAC5-KO. 250 g of protein from freshly harvested tissue from wild-type or HDAC5-knockout hearts was precleared and incubated overnight with 510 g of HDAC5 ( ), HDAC1, 2 ( ), Nkx2.5 ( ), YYI ( ), Sin3a ( ) NCor, CoREST ( ) or IgG ( )-isotype control Ab, Santa Cruz Biotechnology, with constant end-to end rotation. A/G agarose beads were added to each sample and incubated for 1 h, again with end-to end rotation. Beads were harvested and sequentially washed to remove non-specific proteins. Protein complexes were eluted with SDS and resolved on SDS-page gel for western blot analysis using the antibodies against proteins of interest. Western blots shown represent co-immunoprecipitation assays that were done in duplicate and repeated three times.",yes
PMC4217770,Figure_3,oa_package/8e/86/PMC4217770.tar.gz,"['The Ki67-positive rate was 70% and CD45RO was partially positive ().', 'Immunohistochemical staining of case 1 shows positive or partly positive results for (A and B) CD3, (C and D) CD4, (E and F) CD45RO, (G and H) Ki67 and (I and J) TIA-1.']","Figure 3 Immunohistochemical staining of case 1 shows positive or partly positive results for (A and B) CD3, (C and D) CD4, (E and F) CD45RO, (G and H) Ki67 and (I and J) TIA-1. (B, D, F, H and J) are magnified images (scale bar length = 500 m) of (A, C, E, G and I), respectively. Scale bar length: (A, C, G and I) 2 mm and (E) 4 mm. CD, cluster of differentiation; TIA-1, T-cell intracellular antigen-1.",yes
PMC4152685,Figure_1,oa_package/fe/f9/PMC4152685.tar.gz,"['Diagnostic angiogram demonstrated an AVM in the left cerebellum measuring 32 18 22 mm, with feeders from left posterior inferior cerebellar artery (PICA)[a].', 'Preopertive diagnostic subtraction angiogram (DSA) showing AVM in the left cerebellum (a), intraoperative leakage of contrast outside the vasculature (b) and postoperative DSA (c) that shows no residual AVMHalfway through the procedure, there was sudden hypertension (blood pressure (BP) increased from 110/70 to 150/90 mmHg) with bradycardia (heart rate decreased from 120/min to 50/min).', 'A check angiogram showed leakage of contrast outside the vasculature [b].', 'Check angiogram revealed no residual AVM [c].']","Figure 1 Preopertive diagnostic subtraction angiogram (DSA) showing AVM in the left cerebellum (a), intraoperative leakage of contrast outside the vasculature (b) and postoperative DSA (c) that shows no residual AVM",yes
PMC10100505,Figure_6,oa_package/b0/2e/PMC10100505.tar.gz,['Comparing focal monopolar PEF ventricle ablation depths (left) and widths (right) with RF.'],"Figure 6 Comparing focal monopolar PEF ventricle ablation depths (left) and widths (right) with RF. , , RF contact forces indicated by low force (LF, 38g), medium force (MF, 1827g), and high force (HF, 4062g). Trial numbers for each test condition are indicated inside the bars. PEF, pulsed electric field; RF, radiofrequency",yes
PMC4234925,Figure_5,oa_package/c8/5d/PMC4234925.tar.gz,"['109; ).', '0% compared to that of intact striatum (, 6).', '(A) Tyrosine hydroxylase (TH) staining in the SND group, showing decreased staining of dopaminergic axons and terminals in the lesioned striatum, and atrophy (a).']","Fig. 5 ( ) Tyrosine hydroxylase (TH) staining in the SND group, showing decreased staining of dopaminergic axons and terminals in the lesioned striatum, and atrophy ( ). Dopaminergic cell count in the SN revealed 74.719.4% losses of TH-positive cells in lesioned SN against contralateral SN ( , rostral; , medial; , caudal sections) (VTA, ventral tegmental area) ( , 10; , , , 40 magnification). ( ) TH staining in the PD group, showing decreased staining of dopaminergic axons and terminals in the lesioned striatum ( ). Dopaminergic cell count in the SN revealed 92.8 2.7% losses of TH-positive cells in lesioned SN against contralateral SN ( , rostral; , medial; , caudal sections) (VTA, ventral tegmental area) ( , 10; , , , 40 magnification).",yes
PMC5192033,Figure_1,oa_package/00/01/PMC5192033.tar.gz,"['Abdominal x-rays revealed dilated loops of small bowel without colonic distension and no free subdiaphragmatic gas ().', 'Dilated loops of small bowel without colonic distension are seen on this abdominal x-ray.', '']",Fig. 1 Dilated loops of small bowel without colonic distension are seen on this abdominal x-ray. There is no subdiaphragmatic free gas present.,yes
PMC6586133,Figure_2,oa_package/de/4d/PMC6586133.tar.gz,['1601058-F0002.'],10.1080/20009666.2019.1601058-F0002,yes
PMC10556324,Figure_13,oa_package/f1/a9/PMC10556324.tar.gz,[],"Fig. 13 Imaging features of orbital invasion. A 27-year-old female patient with adenoid cystic carcinoma. Coronal soft tissue ( ) computed tomography (CT) image shows expansion and complete opacification of the nasal cavity, bilateral ethmoidal air cells and right maxillary sinus. There is destruction of inferior wall of right orbit with suspicious area of intraorbital extension (black arrow). Intact periorbita seen as a hypointense line (white arrow) on T2-weighted magnetic resonance imaging (T2W MRI) ( ) confirms that there is no orbital invasion. ( , ): A 48-year-old man with adenocarcinoma. Coronal soft tissue ( ) CT image shows an ill-marginated mildly heterogeneously enhancing mass centered in bilateral ethmoidal air cells and nasal cavity. There is destruction of right lamina papyracea with loss of fat planes with the medial rectus muscle (curved arrow). Coronal T2W MRI ( ) reveals discontinuity of the right periorbita (block arrow) favoring intraorbital extension.",yes
PMC8186871,Figure_2,oa_package/79/eb/PMC8186871.tar.gz,"['showed that the presence of right ventricular wall uptake and/or increase of SUV of right ventricular wall on post-therapy were associated with early cardiotoxicity among 121 breast cancer patients who underwent anthracycline or trastuzumab 56 ( 2).', 'Association between increased 18FDG uptake in the right ventricle with diffuse left ventricular involvement as a marker of early chemotherapy-induced cardiotoxicity in patients with breast cancer.']",Figure 2 Association between increased 18FDG uptake in the right ventricle with diffuse left ventricular involvement as a marker of early chemotherapy-induced cardiotoxicity in patients with breast cancer. Anthracycline and trastuzumab were associated with various patterns of 18FDG uptake on PET imaging in the left and right ventricles. Patients with right ventricular involvement were more likely to have diffuse left ventricular involvement and subsequently more likely to have chemotherapy associated with cardiotoxicity. Reproduced with permission from Kim et al. (Figure ),yes
PMC6037300,Figure_3,oa_package/b9/5a/PMC6037300.tar.gz,"['2, ROSAKIT WT, and ROSAKIT D816V cells ().', 'R763 cooperates with PKC412 and with dasatinib in producing growth inhibition in neoplastic mast cells (MC)A: HMC-1.']","Figure 3 R763 cooperates with PKC412 and with dasatinib in producing growth inhibition in neoplastic mast cells (MC) A: HMC-1.1 and HMC-1.2 cells (upper panel) were incubated with various concentrations of R763, PKC412, or a combination of both drugs a fixed ratio of drug concentrations for 48 hours. Lower panel: HMC-1.1 and HMC-1.2 cells were incubated in various concentrations of R763, dasatinib, or a combination of both drugs at a fixed ratio of drug concentrations for 48 hours. Results are expressed as percent of control and represent the meanS.D. of triplicates. B: ROSA and ROSA cells (upper panel) were incubated with various concentrations of R763, PKC412, or a combination of both drugs at a fixed ratio of drug concentrations for 48 hours. Lower panel: ROSA and ROSA cells were incubated with various concentrations of R763, dasatinib, or a combination of both drugs at a fixed ratio of drug concetrations for 48 hours. Results are expressed as percent of control and represent the meanS.D. of triplicates.",yes
PMC6206209,Figure_10,oa_package/8e/77/PMC6206209.tar.gz,[],Figure 10 Co-localization of -catenin and pIGF-IR in MG63 cells using immunofluorescence. B-catenin (red; anti-mouse Alexa Fluor 555) and pIGF-IR (green; anti-rabbit Alexa Fluor 488) protein staining of cells and respective nuclear staining (using TO-PRO-3) were evaluated in cultures after 48 h of incubation with 0% FBS-medium (control) or biglycan (10 g/ml). Negative controls were used wherethe primary antibodies were omitted for both secondary antibodies used (anti-mouse - negative red; anti-rabbitnegative green). Slides were analyzed by confocal microscopy and pictures were taken using x40 magnification.,yes
PMC8380417,Figure_8,oa_package/cd/41/PMC8380417.tar.gz,"['8) [76].', 'Arrhythmogenic mitral valve prolapse.', 'At elettroanatomic mapping, ventricular fibrillation was inducible, with subsequent implantation of a cardioverter defibrillatorIn particular, Carmo et al.']","Fig. 8 Arrhythmogenic mitral valve prolapse. Cine images of a 45-years-old female with frequent BEV and non-sustained ventricular tachycardia (VT), show bileaflet mitral valve prolapse, mitroanular disjunction (arrow in ), systolic curling of lateral LV wall (arrow in ) and a small jet of mitral valve regurgitation (asterisks in ). LGE with non-ischemic pattern involves the inferior and infero-lateral basal wall (arrows in ). At elettroanatomic mapping, ventricular fibrillation was inducible, with subsequent implantation of a cardioverter defibrillator",yes
PMC9361872,Figure_5,oa_package/35/0f/PMC9361872.tar.gz,"['In other preparations, like the one shown in , brief (0.']","FIGURE 5 Omethoate induces calcium bombs in some muscle fibers. Montage of sequential images of the endplate region of a group of FDB muscle fibers before during and after brief (20 Hz, 0.5 s) tetanic stimulation of the tibial nerve supply. Plot of relative increase in fluorescence during the image sequence shown partly in panel (complete sequence in ). Stimulation produced a relatively small increase in fluorescence during stimulation (horizontal bar) but which continued to increase, evidently regeneratively, after cessation of the tetanic stimulus train.",yes
PMC4031977,Figure_4,oa_package/22/fe/PMC4031977.tar.gz,['DNA-Sm14/DNA-Hsp65 immunization reduced tissue damage during granuloma formation in the liver of Schistosoma mansoni infected mice.'],"Figure 4 Representative photomicrography of HE, Picrosirius and -SMA staining from liver sections of mice without immunization (Control), immunized with DNA-Sm14 or DNA-Sm14/DNa-Hsp65, 69days after infection. HE and Picrosirius staining were used to analyze granuloma formation and collagen deposition respectively. Immunohistochemical staining was performed to detect -SMA fibers around the granuloma. Original magnification, X50.",yes
PMC9394199,Figure_4,oa_package/3c/a1/PMC9394199.tar.gz,"['(A) Histopathology showing flattened rete ridges of the epidermis and marked activity in the upper dermis (H E, 4 ).']","Figure 4 (A) Histopathology showing flattened rete ridges of the epidermis and marked activity in the upper dermis (H&E, 4). (B) Epidermal thinning and dermal colonies (black arrow) with dense surrounding inflammation. A histiocytic giant cell (circled with black) noted in the upper half of the image at the edge of dermis (H&E, 10). (C) Colonies (black arrow) immediately surrounded by neutrophilic microabscess, along with surrounding epithelioid histiocytes, noted in the left lower edge and right upper edge of the field (H&E, 40). (D) Another colony (black arrows) surrounded by neutrophils, histiocytes and histiocytic giant cells. The giant cells are marked by black asterisk (H&E, 40).",yes
PMC4214446,Figure_4,oa_package/04/0e/PMC4214446.tar.gz,"['0-mm mass located in the right atrial wall area ().', 'Tomographic ultrasound imaging results demonstrating a solitary tumor (indicated by pointers) located in the right atrium near the right atrial appendage (lower panels).']",Figure 4 Tomographic ultrasound imaging results demonstrating a solitary tumor (indicated by pointers) located in the right atrium near the right atrial appendage (lower panels).,yes
PMC9435559,Figure_2,oa_package/64/e2/PMC9435559.tar.gz,"['9 mm in diameter, located near the right A1 A2 junction ().']",FIG. 2. Three-dimensional rotational angiography from left common carotid arterial injection.,yes
PMC4362789,Figure_3,oa_package/4f/f6/PMC4362789.tar.gz,"['Neurons from / mice displayed higher levels of surface APP than those from +/+ mice (c).', 'Consistent with this observation, DISC1 knockdown (D1) in primary neurons from +/+ mice increased the cell-surface APP immunoreactivity (d).', 'Furthermore, exogenous supplement of full-length DISC1 (DISC1) in primary neurons from / mice normalized the levels of cell-surface APP (e).', 'APP trafficking is regulated by DISC1(a) Knockdown of DISC1 by D1 alters cell surface levels of APP, compared to Con.']","Figure 3 APP trafficking is regulated by DISC1 Knockdown of DISC1 by D1 alters cell surface levels of APP, compared to Con. This effect is rescued by co-expression of R1, but not NR1. Western blotting for APP was done in lysates from mature primary cortical neurons. Surface APP was isolated by biotinylation and precipitation with neutravidin-conjugated beads. Densitometric analysis of surface APP in primary neurons expressing Con, D1, D1+R1, or D1+NR1. Neurons from / mice display higher levels of surface APP than those from +/+ mice. Scale bar, 10 m. Knockdown of DISC1 by D1 increases trafficking of APP to the cell surface. Transfection of mature primary neuron cultures from +/+ mice with D1 increased surface APP (top panels) and decreased internalized APP (3rd from top), compared with Con. Scale bar, 20 m. Expression of exogenous full-length DISC1 rescues the increased trafficking of APP to the cell surface in cells from / mice. Transfection of mature primary neuron cultures from / mice with a DISC1 construct (DISC1) decreased surface APP (top panels) and increased internalized APP (3rd from top), compared with the empty vector (Con). Scale bar, 20 m. Bars represent the meanSEM for at least five independent experiments. * <0.05, ** <0.01, *** <0.001.",yes
PMC6891921,Figure_7,oa_package/bc/9a/PMC6891921.tar.gz,['Neutrophil extravasation corresponds with microvessel permeability.'],"Figure 7 Neutrophil extravasation corresponds with microvessel permeability. a) Triculture vessels are shown perfused with neutrophils and simultaneously with 10 kDa dextran (blue). HUVECred, Pericytesgreen, PMNswhite. Arrows indicate extravasated PMNs at 10 min following perfusion. Scale bar is 200 m. b) Image of PMN (white arrow) extravasating from the lumen into the extravascular space. Scale bar is 50 m. cf) Measurements of PMNs exposed to IgG control antibodies, CXCR1/CXCR2 blocking antibodies, or TNF are shown for c) mean speed, d) % extravasation, and e) % transmigration. f) Endothelial barrier function (permeability) is shown to be significantly altered by TNF stimulation. Shown is mean s.e.m. Significance is indicated by ** < 0.01, **** < 0.0001, test. g) Cartoon demonstrating fibroblasts versus pericytes role in our triculture model.",yes
PMC4401285,Figure_1,oa_package/78/13/PMC4401285.tar.gz,"['At the very end, when almost all cytoplasm has been consumed, one encounters evidence of apoptosis: condensation and margination of chromatin, fragmentation of DNA into nucleosome ladders, exteriorization of phosphatidyl serine and (though insect caspases differ from mammalian caspases and are harder to document) apparent activation of a caspase ().']","1 The very late appearance of characteristics of apoptosis in metamorphosing insect cells. Upper row: appearance of lysosomes during the involution of the salivary gland of , which in our hands has completely collapsed by 13 hrs after the beginning of puparium formation. Monodansylcadaverine detects no resolvable organelles prior to onset of metamorphosis, but by 6 hrs there are numerous perinuclear autophagic vacuoles (arrows) and by 9 hrs the vacuoles fill the cytoplasm (three figures on left). Lysotracker red detects modest activity by 9 hrs and large autophagic vacuoles by 12 hrs. Middle row: By 3 hrs into metamorphosis, the fine filamentous actin network, detected by rhodamine phalloidin, has given way to fine granular clumps of actin, which become more pronounced by 9 hrs. By 10 hrs the actin is in vacuoles that appear to be lysosomes, or clustered near the cell membranes. Lower row: Evidence for apoptosis occurs only very late. Both exteriorization of phosphatidylserine as detected by annexin V (left two panels) and appearance of TUNEL-positive nuclei (right two panels) occur after the 11 hr.We also confirmed the presence of caspase-positive granules at 12 hrs but, failing an interpretation of the significance of the granules, we withhold an interpretation. From doctoral research of Farhan S. Khan.",yes
PMC8572705,Figure_6,oa_package/e8/76/PMC8572705.tar.gz,"['We also performed an IHC assay to monitor IL-13 expression in liver tissue (', ') (', '01) (', '']",Fig.5 (A). The ratio of MMP-12 to actin are presented as meanSEM from 3 liver specimens in each group. (a: <0.01; vs. CON); (b: <0.01; vs. OVX); (c: <0.01; vs. OVX+HFHF); (d: <0.01; vs. OVX+HFHF+GEN). (B). The ratio of HDAC3 to actin are presented as meanSEM from 3 liver specimens in each group. (a: <0.01; vs. CON); (b: <0.01; vs. OVX); (c: <0.01; vs. OVX+HFHF); (d: <0.01; vs. OVX+HFHF+GEN).,yes
PMC11532336,Figure_2,oa_package/51/66/PMC11532336.tar.gz,"[' 2A).', ' 2B, of all cytokines tested, IL-4 and IL-13 markedly stimulated the expression of all Gsdmc isoforms.', ' 2C-D and Supplementary ', ' 2E-F).', ' 2G).', ' 2H).', '\nGsdmc1-4 is upregulated in vivo and in vitro in response to Th2 cytokines.', '\nMice lacking the expression of Gsdmc genes are viable and show no overt phenotypeIn order to determine the functional significance of the Gsdmc1-4 genes in intestinal homeostasis and disease, we generated a novel mouse line.', ' 2A), the expression of Gsdmc1-4 is profoundly diminished in cultured IECs, likely due to the absence of factors produced by non-epithelial cells and present in the intestinal microenvironment.']","Fig. 2 is upregulated and in response to Th2 cytokines. ( ) Expression levels of in freshly isolated IECs and in intestinal organoids. ( ) , , and counts in intestinal organoids stimulated with 50 ng/ml of the indicated cytokines for 24h. Level of GSDMC2+3 proteins in intestinal organoids stimulated with IL-4 (50 ng/ml, 24h) ( ) and IL-13 ( ) (50 ng/ml, 24h) (Western blot image represents cropped gel where irrelevant samples and lanes were removed for clarity. Original blots/gels are presented in Supplementary Fig.4A-B). -actin was used as a loading control. ( ) Relative transcript levels of , , , and in colon tissue from mock and mc-IL13 injected mice. ( ) Photomicrographs and insets of mock and mc-IL13 injected mice stained for GSDMC4 (white) in colon tissue. Counterstaining with Hoechst (blue). Scale bar 100m and 50m (inset). ( ) Heat map depicting , , and expression in proficient and deficient small intestinal organoids in response to IL-13 and IL-4 (50 ng/ml, 24h). ( ) Scheme of the modulation of genes in response to type 2 cytokines. *, ** and *** indicate the <0.05, <0.01 and <0.001, respectively from Students t-test or Two-Way ANOVA.",yes
PMC3868051,Figure_4,oa_package/ed/c0/PMC3868051.tar.gz,"['Kidneys from nondiabetic GLO1-KD (Aband B, bar 2) showed a nearly twofold increase in thickness of the GBM compared with nondiabetic Wt mice (', 'In these nondiabetic mice, the degree of increased GBM thickness was identical to that found in diabetic Wt mice (Adand B, bar 4).', 'In contrast, GBM thickness in kidneys of diabetic GLO1-Tg mice (Afand B, bar 6) was the same as in nondiabetic Wt mice.', 'Measurement of GBM thickness (n = 15).', 'To evaluate whether the previously reported alterations in proteasomal activity and promoter histone 3 at lysine 4 (H3K4) monomethylation seen in diabetes contribute to the renal pathological and functional changes observed in GLO1-KD mice, we measured both proteasomal activity and H3K4 me1 in Wt and GLO1-KD mice.']","Figure 4 Measurement of GBM thickness ( = 15). : Representative electron micrographs of glomeruli from kidneys of nondiabetic and diabetic Wt ( ), GLO1-KD ( ), and GLO1-Tg mice ( ). Original magnification 12,000. : Quantitation of GBM thickness. Data are expressed as mean SD (* 0.05 vs. Wt; # < 0.05 vs. STZ-Wt; ANOVA).",yes
PMC3925298,Figure_2,oa_package/d5/c4/PMC3925298.tar.gz,"[' 2a).', ' 2b).', '', 'CD mice in each time point by ANOVA and Wilcoxon test)\nLipid accumulation and FFA components during liver regeneration after PHxLipid accumulation into hepatocytes was shown during liver regeneration in both groups.']","Fig.2 Liver mass regeneration ratio and serum liver function examinations. Liver mass regeneration ratio during liver regeneration. The change of plasma ALT, total bilirubin (T-Bil), and albumin (Alb) levels during liver regeneration ( Control, HFD, =58; meanSE, * <0.05 HFD mice vs. CD mice in each time point by ANOVA and Wilcoxon test)",yes
PMC11330038,Figure_2,oa_package/80/04/PMC11330038.tar.gz,"[' 2).', '\nIllustrative diagram of temporal lobe sampling at the UC Davis ADRC Neuropathology Core.', '503 m/pixel, to produce a Whole Slide Image that is generated as a CSV file and can be analyzed and annotated in the Aperio Image Scope software\n\nTable 2Count of plaques and cerebral amyloid angiopathy (CAA) by Alzheimer s Disease Neuropathologic Change (ADNC)No/Not ADNC (n = 16)Low ADNC (n = 30)Intermediate ADNC(n = 23)High ADNC (n = 62)P value\nGray Matter\nCored Plaques, raw count, median (CI)0.']","Fig. 2 Illustrative diagram of temporal lobe sampling at the UC Davis ADRC Neuropathology Core. Coronal brain sections of the temporal gyri (middle and superior), approximately 1cm thick, are obtained and sampled at the level of the insula and hippocampus. The histopathological analysis encompasses the superior and middle temporal gyri, which, after sampled, are paraffin-embedded, cut at 5 to 7m thick, mounted in glass slides, stained, and scanned at a 20x magnification, at a rate of 0.503m/pixel, to produce a Whole Slide Image that is generated as a CSV file and can be analyzed and annotated in the Aperio Image Scope software",yes
PMC9582844,Figure_5,oa_package/17/f1/PMC9582844.tar.gz,[],"Figure5 Association between CRPGS, androgen response pathway, and ADT response. Results of GSVA between high- and low-risk PCa patients , and the Venn diagram showed the common enriched and decreased pathways . The estimated IC50 of bicalutamide between high- and low-risk patients in TCGA-PRAD cohort . The alterations in the expression level of CXCL14, CCL20, CCL24, and CCL26 after ADT treatment in the GSE150368 cohort . The association between CRPGS, ARA score, and ARSI exposure status , and the estimated IC50 of bicalutamide between high-and low-risk patients in Abida et als cohort . *p < 0.05; ns, not significant.",yes
PMC2612686,Figure_1,oa_package/c4/d7/PMC2612686.tar.gz,['Pre-operative abdominal computed tomography scan of the patient demonstrating characteristic features of mesenteric panniculitis; soft tissue density/mass (asterisk) and relative sparing of mesenteric vessels (grey arrow).'],Figure 1 Pre-operative abdominal computed tomography scan of the patient demonstrating characteristic features of mesenteric panniculitis; soft tissue density/mass ( ) and relative sparing of mesenteric vessels ( ).,yes
PMC11360668,Figure_2,oa_package/69/66/PMC11360668.tar.gz,['Postoperative CT scan of an 85-year-old woman showing the performed decompression in the coronal plane (red arrow) and axial plane (white arrow) at the L3-L4 level.'],Figure 2 Postoperative CT scan of an 85-year-old woman showing the performed decompression in the coronal plane (red arrow) and axial plane (white arrow) at the L3-L4 level. A: Coronal plane; B: axial plane.,yes
PMC7064898,Figure_5,oa_package/39/3e/PMC7064898.tar.gz,['\nSignificant reduction of PV expressing cells in the perirhinal cortex of AD patients.'],"Figure 5 Light microscopy images of PV immunoreactivity in the perirhinal cortex of Braak II (a1 anda4), Braak IIIIV (a2 anda5) and Braak VVI (AD patients; a3 anda6) postmortem samples. PVpositive somata (arrows) and nerve fibers, mainly located in layer III, are clearly reduced in Braak IIIIV and Braak VVI cases compared to agematched Braak II individuals, as observed in high magnification images (a4a6). Some dystrophic neurites appear in the nearness of an Abeta plaque (inset in a6, asterisk indicates an unstained Abeta plaque). Open white circles indicate amyloid plaques location. Stereological counts of PVpositive interneurons reveal a significant decrease in the numerical density (cells/mm ) of this inhibitory population in the Braak VVI perirhinal cortex (n=35). Data (meanSD) were analyzed by oneway ANOVA <0.006 ( (2, 10)=8.741 followed by Tukey post hoc multiple comparison test. Significance (** <0.01) is indicated in the figure. Scale bars: a1a3, 200m; a4a6, 50m; inset in a6, 20m.",yes
PMC3156516,Figure_1,oa_package/8a/32/PMC3156516.tar.gz,"['281013Saline infusion sonography demonstrating posterior wall uterine polyp, protruding into the cavity, measuring 15 10 mm.']","Figure 1 Saline infusion sonography demonstrating posterior wall uterine polyp, protruding into the cavity, measuring 1510 mm.",yes
PMC11670280,Figure_5,oa_package/19/40/PMC11670280.tar.gz,['Indirect co culture of RFP HUVECs and PDLCs.'],"Figure 5 Indirect coculture of RFP HUVECs and PDLCs. A) microfluidic device with five separated channels. The right sketch depicts the dimensions with the central part, where vasculature forms, flanked by two medium channels and the outermost chambers filled with PDLCs. B) Fluorescent image of RFP HUVECs (red) in central chamber during static condition resulting in network formation. Furthermore, a fluorescent 1m bead suspension is supplied via the side channels. The beads are clearly visible in the central chamber inside vessel, but they do not appear in the opposite side channel indicating that the network is not completely perfusable. C) A fluorescent image of RFP HUVECs (red) in the central chamber during medium perfused culture condition (60Lh ) resulting in no network formation. Fluorescent images are confocal stacks presented as 2D maximum projections. Scale bars: 100m.",yes
PMC3843686,Figure_1,oa_package/17/d9/PMC3843686.tar.gz,"['In contrast to the diffuse cytoplasmic staining of mCherry, transiently transfected TDP-43-mCherry constructs resulted in punctate fluorescence confined to, but dispersed throughout, the nucleus with exclusion from nucleoli (A) This distribution is similar to previous observations for endogenous TDP-43 [50] and confirms correct targeting of the fusion proteins (A).', 'g001ER stress and proteasome inhibition cause redistribution of wildtype and ALS-linked mutant TDP-43.', 'g001""/>There was moderate cytoplasmic fluorescence in approximately 10 20% of cells expressing either wildtype or mutant TDP-43-mCherry (C).', 'Wildtype and mutant TDP-43-mCherry proteins ( 70 kDa) were detected at levels similar to the endogenous protein with a transfection efficiency of approximate 50%, suggesting an approximate two-fold expression level of the fusion protein compared to endogenous in transfected cells (B).', 'Cytoplasmic TDP-43-mCherry was detected in approximately 50% of wildtype and mutant TDP-43 expressing cells treated with thapsigargin, compared with 10 20% of untreated cells (D).', '0081170-Lee1"" ref-type=""bibr"">[51] (E).']",10.1371/journal.pone.0081170.g001,yes
PMC9478670,Figure_2,oa_package/a5/86/PMC9478670.tar.gz,"[' 1 and ', ' 1', ' 2 is a lateral lumbar spine conventional radiograph that demonstrates multiple vertebral body compression fractures visualized on a background of diffuse osseous demineralization to include a severe compression fracture ( 40% height loss) at L3 (arrow).']",Fig.2 Lumbar spine radiograph: Conventional lateral radiograph of the lumbar spine demonstrates multiple vertebral body compression fractures visualized on a background of diffuse osseous demineralization to include a severe compression fracture (>40% height loss) at L3 (arrow).,yes
PMC11241716,Figure_1,oa_package/61/58/PMC11241716.tar.gz,"['Paraoesophageal varices, frequently associated with esophageal varices, are venous dilatations that extend outside the esophageal wall and appear on post-contrast CT examinations as tortuous vessels extending along the distal esophagus (A).', 'Gastric varices can be observed at the postero-superior portion of the gastric fundus (B).', 'They can develop in isolation, but most often, gastric and esophageal varices are present simultaneously and are considered a separate class called gastro-esophageal varices (C).', 'These varices are visible on post-contrast CT examinations, appearing as an enlarged and tortuous left gastric vein at the lesser curvature of the stomach and the posterior wall of the left hepatic lobe (see D).', '1007/s12072-022-10377-w35941400\n(A) CT appearances of esophageal varices show as intraluminal protrusion with scalloped margins (large arrow).']","Figure 1 ( ) CT appearances of esophageal varices show as intraluminal protrusion with scalloped margins (large arrow). Paraoesophageal varices (thin arrows) appear as well-defined, round, tubular, or serpentine structures situated outside the wall of the esophagus in the mediastinum. ( ) Gastric varices (large arrow) located submucosal in the postero-superior aspect of gastric fundus and presence of dilated veins on the lesser curvature of stomach (thin arrows). ( ) Left coronary vein varices (large arrow). ( ) Gastroesophageal varices extend from inferior esophagus to the upper pole of the stomach (large arrows), in association with paraoesophageal varices (thin arrow).",yes
PMC10898897,Figure_2,oa_package/7c/aa/PMC10898897.tar.gz,"['Contrast-enhanced USGNon-TNBC subtype displayed a higher tendency to present with diffuse enhancement which could be heterogeneous or homogeneous ().', 'Table 1.']","Figure 2. CEUS for non-TNBC subtype breast cancer-Grey scale USG image of a diagnosed HER 2 enriched subtype of breast cancer (a): Shows an irregular hypoechoic mass with angular margins and microcalcifications (arrow) at 12 o clock position in right breast. Dual display CEUS images at 30 seconds (b): Shows avid uniform enhancement of the entire lesion, which suggested diffuse homogeneous pattern. At 90 seconds (c): There is heterogeneous washout within the lesion. (d): Quantitative display showed peak intensity of 70%, TTP of 34 seconds, AUC of 6,600 and MTT of 71 seconds.",yes
PMC2804724,Figure_3,oa_package/e6/ea/PMC2804724.tar.gz,"['Histological appearance of the lesion in Case 1: the neoplasm consists of a sub-mucosal, cellular aggregation of spindle-shaped, fibroblast-like cells with relatively pale, ovale nuclei; scattered round histiocytic cells are also present (Haemotxylin and Eosin original magnification 20).']",Figure 3 .,yes
PMC6567937,Figure_1,oa_package/6a/ed/PMC6567937.tar.gz,"['In order to fully adhere to hospital protocol the marker ball must be present in its entirety this is because the templating software can only calibrate the X-rays if the entire ball is present in the image (figure 1).', 'An example of an anteroposterior (AP) pelvic radiograph taken for a suspected neck of femur fracture in the emergency department.']",Figure 1 An example of an anteroposterior (AP) pelvic radiograph taken for a suspected neck of femur fracture in the emergency department. At the bottom of the image is an example of a well-placed marker ball.,yes
PMC3644251,Figure_3,,"['Figure 3Representative immunohistochemistry staining for CD3, CD8 and CD45RO and each cancer type; 5× magnification.']",,yes
PMC9700497,Figure_3,,"['04"">Inflammatory response and immune tolerance in macaque liverHepatocytes, which were the predominant cells in the liver (\nFigure 3A–C), were enriched in\nACE2 and\nTMPRSS2 coexpression, especially zone 1 hepatocytes (\nFigure 3D), and contained the most DEGs between infected and healthy livers (\nFigure 3E; Supplementary Table S6).', 'Although the proportion of coexpression was notably higher in the healthy control cholangiocytes, the proportion in infected samples was zero (\nFigure 3D), which may be due to the extremely low number of cholangiocytes captured in infected samples (\nFigure 3C).', 'Figure 3Figure 3Hepatocyte dysregulations and immune evasion pathway in the liver of SARS-CoV-2-infected macaquesA: Clusters and respective cell type assignments in UMAP.', 'DEG enrichment analysis in hepatocytes indicated that the complement system was activated in infected samples, as evidenced by the activation of various pathways, including the classical, alternative, and lectin-induced complement pathways (\nFigure 3F).', '“Response to cytokine” was also enriched in the up-regulated genes of hepatocytes (\nFigure 3F).', 'Accordingly, the expression levels of\nSTAT3,\nNFKB1,\nNFKB2,\nRELB, and\nREL were also up-regulated (\nFigure 3G).', 'Various metabolic processes, including those involving lipids, lipoproteins, fatty acids, glucose, and glycogen, were dysregulated in the liver after SARS-CoV-2 infection (\nFigure 3H; Supplementary Figure S6A).', 'Here, bile biosynthesis and secretion-related processes were significantly enriched in down-regulated genes in hepatocytes (\nFigure 3H).', 'For example, the expression levels of genes encoding membrane transporters involved in bile secretion, such as\nABCB1,\nABCC3,\nABCC6,\nSLC22A1, and\nSLC22A7 (\nBoyer, 2013), were decreased in the hepatocytes (\nFigure 3I), indicating that bile generation and secretion may be impaired in the liver after SARS-CoV-2 infection.', 'In this study, we found extensive dysregulation of coagulation factors, inhibitors, and fibrinolysis-associated proteins in the liver after infection (\nFigure 3J), especially in hepatocytes and endothelial cells, which are the primary sources of these coagulation factors.', ', 2007), the active form (plasmin) of which plays a key role in fibrinolysis by dissolving fibrin in blood clots, was up-regulated (\nFigure 3J).', 'However, we found no activation signatures of macrophages or T cells, other than up-regulation of complement components (\nFigure 3K, L).', 'Here, the expression levels of\nIDO2 and\nTDO2 were elevated in hepatocytes (\nFigure 3F), and\nIDO2 was also elevated in macrophages and T cells after SARS-CoV-2 infection (\nFigure 3K, L).', 'Moreover, Kyn 3-monooxygenase (KMO), which catalyzes the hydroxylation of Kyn to form 3-hydroxykynurenine (3-HK), and Kyn aminotransferase 3 (KYAT3), which catalyzes the transamination of Kyn to form kynurenic acid (KynA), were up-regulated at the transcriptional level in hepatocytes (\nFigure 3F).', 'KMO gene expression was also elevated in immune cells (\nFigure 3K, L).', 'Along with Kyn accumulation resulting from increased dioxygenases,\nAHR gene expression was up-regulated in hepatocytes (\nFigure 3F), macrophages (\nFigure 3K), and T cells (although not significantly in T cells).', 'Consistent with the immunosuppressive effect described above, the proportions of macrophages and T cells were reduced in the infected liver samples (\nFigure 3C), and genes associated with apoptosis, including\nCRADD,\nGAS2,\nFHIT, and\nZNF385B, were up-regulated in these immune cells (\nFigure 3K, L).', 'As described previously, cytokine receptor genes, signal transducer genes, NF-κB subunit genes, and cytokine and chemokine genes, such as\nIL1A,\nIL6,\nCXCL2, and\nCXCL10, were up-regulated in the liver (\nFigure 3G, K, L; Supplementary Figure S6B).', 'These mechanisms are summarized in\nFigure 3M, providing new insights into liver manifestations and novel immunomodulatory therapies for SARS-CoV-2 infection.']",,yes
PMC11315081,Figure_2,error_images/gr2.jpg,"['115 articles were eligible for the study of extracting the related information after reading the abstract and the full text (details in the following flowchart Fig. 2).', 'Fig. 2Fig.']",,yes
PMC7907007,Figure_2,error_images/gr2.jpg,['Biopsies of the oral and digital ulcerations performed by otolaryngology and dermatology showed granulation tissue and epidermal spongiosis consistent with reactive changes (Fig 2).'],,yes
PMC11626646,Figure_2,error_images/gr2.jpg,"['Similar morphology was noted on the radial margins of both palms (Fig 2), along with thickened clusters of hyperpigmented papules on the knuckles (Fig 3).']",,yes
PMC9893553,Figure_2,error_images/gr2.jpg,"['The dermoscopic features observed in our reported case are demonstrated in Fig 2.', '2Fig 2B.']",,yes
PMC7509353,Figure_2,error_images/gr2.jpg,['Histopathology found columns of compact hyperkeratosis and parakeratosis with underlying hypogranulosis and without underlying dyskeratosis in the spinous layer (Fig 2).'],,yes
PMC9663740,Figure_2,error_images/gr2.jpg,"['Skin punch biopsy demonstrated diffuse dermal microthrombi, endothelial cell damage, and red blood cell extravasation, without perivascular inflammation (Figs 2 and 3).']",,yes
PMC11404049,Figure_2,error_images/gr2.jpg,"['Shave biopsies demonstrated an intraepidermal collection of pale cells with reniform nuclei (Fig 2) positive for CD207, S100, and CD1a staining (Fig 3).']",,yes
PMC11401095,Figure_2,error_images/gr2.jpg,['A shave biopsy was performed (Fig 2).'],,yes
PMC9449643,Figure_2,error_images/gr2.jpg,[],,yes
PMC11450899,Figure_3,error_images/gr2.jpg,"['The group with the second highest audio-only visit rate was encounters with patients aged 65 to 74, and encounters with patients in the youngest age groups consistently had the lowest audio-only visit rate (Figure 3A).', 'Visit encounters with patients who were non-White consistently had higher audio-only visit rate, though the difference between groups decreased by the end of the study period (Figure 3B).', 'Visit encounters with patients who had Medicaid or Medicare as their primary payer consistently had higher audio-only visit rates across all time periods compared to patients with private insurance (Figure 3C).', 'Visit encounters with patients who lived in areas of the highest SDI (SDI quintile 5) had the highest rate of audio-only visits in all time periods except April-June 2021 (Figure 3D).', 'Figure 3Changes in Rate of Audio-Only Encounters Over Time(A) Age, (B) race, (C) insurance status, and (D) social deprivation index (SDI).']",,yes
PMC11307630,Figure_10,error_images/gr2.jpg,[],,yes
PMC8441098,Figure_2,error_images/gr2.jpg,"['Punch biopsy revealed a papillomatous epidermis with abortive follicles, papillary mesenchyme, and ectopic sebaceous lobules (Fig 2).']",,yes
PMC10494304,Figure_2,error_images/gr2.jpg,"['Grocott methenamine silver stain demonstrated dermal narrow-based budding yeast (Fig 2).', 'Grocott methenamine staining (Fig 2) of the skin biopsy highlighted small narrow-based budding yeast in the dermis consistent with histoplasmosis.', 'Although sarcoidosis can present with subcutaneous nodules as well as systemic symptoms such as fevers, the patient’s resistance to immunosuppression and presence of narrow-based budding on skin biopsy (Fig 2) indicates another cause.']",,yes
PMC8010321,Figure_2,error_images/gr2.jpg,[],,yes
PMC9486367,Figure_2,error_images/gr2.jpg,['Skin biopsy demonstrated diffuse dermal neutrophilic infiltrate with papillary dermal edema and hemorrhage (Fig 2).'],,yes
PMC11208766,Figure_2,error_images/gr2.jpg,[],,yes
PMC11490673,Figure_2,error_images/gr2.jpg,"['Histopathology and special stains were performed (Fig 2, A and B).']",,yes
PMC11198053,Figure_3,error_images/gr2.jpg,"['First, familiarity with identification and management of CIED infections was very low among non-EP cardiologists and PCPs, as shown in the Central Illustration.', 'Central IllustrationGaps and Barriers in CIED InfectionCIED = cardiac implantable electronic device; EMR = electronic medical record; EP = electrophysiologist; PCP = primary care physician.']",,yes
PMC6138843,Figure_2,error_images/gr2.jpg,[],,yes
PMC7452279,Figure_2,error_images/gr2.jpg,"['Punch biopsy and wet mount were obtained (Figs 2 and 3).', 'The characteristic ribbon-shaped or broad branching non–septate hyphae and sporangium (Figs 2 and 3) are classic for mucormycosis.']",,yes
PMC7701008,Figure_2,error_images/gr2.jpg,['The lining cells were atypical with prominent nucleoli (Figs 2 and 3).'],,yes
PMC7935687,Figure_2,error_images/gr2.jpg,[],,yes
PMC8058607,Figure_2,error_images/gr2.jpg,[],,yes
PMC5519992,Figure_3,error_images/joddd-11-117-g003.jpg,"['After obtaining parental consent, it was decided to complete the access (Figure 3a) and biomechanical preparation in the first visit, followed by 3Mix (1:1:1 ratio) placement and closure of the access cavity with zinc oxide-eugenol cement.', 'During subsequent appointment after two days, the canals were thoroughly irrigated with normal saline, followed by drying with paper points and placement of tissue scaffold PerioGlas® (bioglass) and homing of SHED from apex to 5 mm of the access cavity with a 25-gauge needle in the root canals of teeth #31 and #41 (Figure 3b).', 'Figure 3a.']",,yes
PMC5519992,Figure_4,error_images/joddd-11-117-g004.jpg,"['Radiographic examination revealed periapical radiolucency involving both #31 and #41 (Figure 4a).', 'On recall examination after one week, the patient was asymptomatic and reported no pain (Figure 4b).', 'The radiograph showed significant resolution of radiolucency in relation to teeth #31 and #41 from 30-day recall to the 365-day follow-up (Figure 4C and 4d) and responded positively to electric pulp testing from the 90-day to 365-day recall evaluation.', 'Figure 4A: Periapical radiolucency in relation to teeth #31 and #41; B: 7-day post-operative radiograph; C: 30-day follow-up radiograph showing complete resolution of radiolucency in relation to teeth #31 and #41; D: 12-month review radiograph.']",,yes
PMC5519992,Figure_5,error_images/joddd-11-117-g005.jpg,"['Access opening was prepared and the necrotic pulp tissue was removed (Figure 5a).', 'Then, the scaffold PerioGlas® (bioglass) was introduced into the canal, followed by homing of SHED (Figure 5b) and the cavity was sealed with a glass-ionomer cement of 5 mm thickness.', 'Figure 5a.']",,yes
PMC5519992,Figure_6,error_images/joddd-11-117-g006.jpg,"['Radiographically open apex in association with periapical lesion of approximately 2 mm in diameter was seen in #11 (Figure 6a).', 'The patient returned after 7 days; he was asymptomatic, reporting no pain to percussion tests (Figure 6b).', 'Figure 6A: Preoperative radiograph displaying periapical radiolucency in relation to tooth #11; B: 7-day postoperative radiograph; C: 30-days follow-up radiograph showing complete radiolucency in relation to tooth #11.']",,yes
PMC2475460,Figure_1,error_images/ulstermedj00004-0020-a.jpg,"['ImagesFig 1Fig 2Stretching exercisesThe stretching exercises targeted both upper and lower extremities, low back, and neck areas (Figure 1).']",,yes
PMC9893553,Figure_1,error_images/gr1.jpg,"['Physical examination revealed pustules and vesicles with central umbilication at the same stage of development and targetoid crusted ulcers on the right inguinal area (Fig 1), base of the penis, right gluteal region, and left cheek along with bilateral painful inguinal adenopathy.', 'Fig 1\nQuestion 1: Based on the clinical presentation, what is the most likely etiology of the patient’s ulcers?']",,yes
PMC7509353,Figure_1,error_images/gr1.jpg,"['On examination, scattered discrete hyperkeratotic projections were noted on her bilateral palms and soles (Fig 1).', 'Fig 1Fig 2Question 1: What is the most likely diagnosis?']",,yes
PMC9112125,Figure_1,error_images/gr1.jpg,"['We defined the ‘clinical assessment period’ (years 2013 through 2016) as the time window during which EHR data were used to calculate BP variability (Figure 1).', 'For primary analyses, we defined the ‘outcomes surveillance period’ (years 2017 through 2019) as the time window during which ICD-9 and ICD-10 codes were used to identify new-onset cardiovascular events including myocardial infarction, heart failure (both preserved and reduced ejection fraction), and stroke (Figure 1).', 'Figure 1Primary cohort development.', 'Fig 1Sampling strategy.', 'We further excluded 3196 patients aged <18 years at the study period start, resulting in a final sample of 42,482 patients who contributed 1117,944 qualifying BP measurements, of which 600,953 occurred during the clinical assessment period (from 2013 through 2016); BP data from this period were used to calculated variability independent of the mean (VIM) for the primary analysis (Figure 1).', 'In sex-aggregated analyses of specific outcomes, both SBP and DBP VIM were also positively associated with heart failure, stroke and mortality, though only DBP VIM was associated with myocardial infarction (Supplemental Figure 1A,C).', 'There were no sex differences in SBP and DBP VIM associations with the composite or distinct cardiovascular outcomes (Supplemental Figure 1B,D).']",,yes
PMC11198227,Figure_2,error_images/gr1.jpg,[],,yes
PMC9663740,Figure_1,error_images/gr1.jpg,"['Key wordsdrug responsedurvalumabICIimmune checkpoint inhibitorimmune related adverse eventirAEmedical dermatologyoncologyprogressingpurpurarapidlyretiformretiform purpurathrombotic occlusive vasculopathyAbbreviations usedANA, antinuclear antibodyANCA, antineutrophil cytoplasmic antibodiesAFB, acid-fast bacillusAVE, acute vascular eventCT, computed tomographyEKG, electrocardiogramICI, immune checkpoint inhibitorINR, international normalized ratioirAE, immune-related adverse eventPCR, polymerase chain reactionPD-L1, programmed death-ligand 1PTT, partial thromboplastin timeVTE, venous thromboembolismHistoryA 71-year-old female was admitted with respiratory failure and rapidly progressive lower extremity retiform purpura (Fig 1).', 'Fig 1Fig 2Fig 3\nQuestion 1: What is the most likely diagnosis?']",,yes
PMC11404049,Figure_1,error_images/gr1.jpg,"['Six-week follow-up demonstrated progression to eroded and crusted reddish-brown plaques throughout the groin, suprapubic abdomen, anterior trunk, upper mid-back, and neck (Fig 1).', 'Fig 1Fig 2Fig 3\nQuestion 1: What is the most likely diagnosis?']",,yes
PMC11401095,Figure_1,error_images/gr1.jpg,"['8 cm dark purple nodule on the distal right great toe extending beneath the nail (Fig 1).', 'Fig 1Fig 2\nQuestion 1: What is the patient’s diagnosis?']",,yes
PMC9449643,Figure_1,error_images/gr1.jpg,"['At the periphery of tumor islands, the basaloid cells were palisaded (Figs 1 and 2).', 'Fig 1Fig 2\nQuestion 1: What is the diagnosis?']",,yes
PMC11450899,Figure_2,error_images/gr1.jpg,"['4% in October-December 2021), and also when analyzed over time by age, insurance status, race, and SDI (Figure 2).', 'Figure 2Changes in Rate of Audio-Only VersusVideo Visit Encounters Over Time From January 2020 to December 2021 by QuarterVisit encounters with patients age 75+ consistently had the highest audio-only visit rate.']",,yes
PMC11198041,Figure_2,error_images/gr1.jpg,[],,yes
PMC8441098,Figure_1,error_images/gr1.jpg,"['Physical examination revealed a 4-cm exophytic, erythematous, friable, and cerebriform nodule on the right frontoparietal aspect of the scalp (Fig 1).', 'Fig 1Fig 2\nQuestion 1: What is the most likely diagnosis?']",,yes
PMC8010321,Figure_1,error_images/gr1.jpg,"['Physical examination demonstrated non-blanching, scaly erythematous papules and plaques on his legs, right arm, and trunk (Figs 1 and 2).', 'Fig 1Fig 2Fig 3Question 1: Based on the clinical presentation, history, and histologic findings, what is your diagnosis?']",,yes
PMC9486367,Figure_1,error_images/gr1.jpg,"['Examination showed multiple nonblanching dark red papulovesicles coalescing into plaques on the bilateral dorsal hands (Fig 1).', 'Fig 1Fig 2\nQuestion 1: What is the most likely diagnosis?']",,yes
PMC11208766,Figure_1,error_images/gr1.jpg,"['Upon examination, bilateral tightly roofed vesicles on his hands, along with milium-grain scars (Fig 1), crusted erosions on the face and hands (Figs 1 and 2), bilateral malar hypertrichosis (Fig 3), diffuse skin pigmentation, and port-red urine were observed.', 'Fig 1Fig 2Fig 3\nQuestion 1: What is the likely diagnosis?']",,yes
PMC11490673,Figure_1,error_images/gr1.jpg,"['Key wordsactinomycosisanaerobic bacteriadental infectionhistopathologymycetomapathologypustulesskin infectionAbbreviation usedTMP-SMXtrimethoprim-sulfamethoxazoleCase reportA 27-year-old multigravida female at 22 weeks gestation presented to the clinic for evaluation of an 8-month history of painful, erythematous pustules and indurated plaques to the right cheek (Fig 1).', 'Fig 1Fig 2\nQuestion 1: Which of the following is the most likely diagnosis?']",,yes
PMC6138843,Figure_1,error_images/gr1.jpg,"[""Key wordserythrasmageneral dermatologymedical dermatologyA 59-year-old woman with type II diabetes mellitus and obesity presented to the clinic for evaluation of a hyperpigmented rash located in her axillae, groin, buttock, umbilicus, and inframammary region (Fig 1, Fig 2, Fig 3, Fig 4, Fig 5, Fig 6) of 1 years' duration."", 'Fig 1Fig 2Fig 3Fig 4Fig 5Fig 6Question 1: What is the diagnosis?']",,yes
PMC7452279,Figure_1,error_images/gr1.jpg,"['The patient had enlarging pink-to-violaceous dusky necrotic plaques of the left breast (Fig 1).', 'Fig 1Fig 2Fig 3Question 1: What is the best diagnosis?']",,yes
PMC10363656,Figure_1,error_images/gr1.jpg,"['Physical exam revealed hyperkeratotic, hyperpigmented plaques with erosions in a geometric pattern within the new tattoos on his dorsal feet, which gradually spread along his shins ending below his knees (Fig 1).', 'Fig 1\nQuestion 1: What is the most likely clinical diagnosis?']",,yes
PMC11198128,Figure_1,error_images/gr1.jpg,"['\n\nConclusions and a call to actionRecruitment and retention of women trainees are more important than ever to reduce sex disparities in cardiology (Figure 1).', 'Figure 1Areas to Address for Recruitment and Retention of Female Trainees From theTrainee PerspectiveFunding support and author disclosuresThe authors have reported that they have no relationships relevant to the contents of this paper to disclose.']",,yes
PMC7701008,Figure_1,error_images/gr1.jpg,"['5-cm subcutaneous blue nodule on the vertex of his scalp gradually increasing in size over several years (Fig 1).', 'Fig 1Fig 2Fig 3Question 1: Which of the following is the most likely diagnosis?']",,yes
PMC7935687,Figure_1,error_images/gr1.jpg,"['Physical examination demonstrated depigmented patches on the lower extremities with poorly circumscribed, feathered edges (Figs 1 and 2).', 'Fig 1Fig 2Fig 3Question 1: What is the most likely diagnosis?']",,yes
PMC8058607,Figure_1,error_images/gr1.jpg,"['On physical examination, the patient appeared well, with scattered, variably sized, purpuric patches and urticarial plaques on the torso and extremities (Fig 1, Fig 2, Fig 3).', 'Fig 1Fig 2Fig 3Question 1: What is the most likely diagnosis?']",,yes
PMC7797925,Figure_1,error_images/gr1.jpg,"['A physical exam demonstrated firm, non-pitting edema and hyperpigmentation of his bilateral lower legs with many verrucous papules and plaques (Fig 1).', 'Fig 1Fig 2Fig 3Question 1: Based on the clinical presentation, history, and histology, what is your diagnosis?']",,yes
PMC11626646,Figure_3,error_images/gr3.jpg,[],,yes
PMC11308027,Figure_3,error_images/gr3.jpg,"['017]), (Figure 3).', 'Figure 3Boxplot of Neck Disability Index (NDI) comparing baseline to follow-up for the treatment group.']",,yes
PMC9893553,Figure_3,error_images/gr3.jpg,"['\n\nQuestion 3: A biopsy of a right inguinal papule revealed an orthokeratotic stratum corneum with a preserved granular layer, ballooning degeneration, spongiosis, karyorrhexis, neutrophilic infiltrate, and intraepithelial cytoplasmic inclusion bodies (\nFig 3\n).', 'No stain or additional testing needed\nFig 3\nAnswers:\nA.']",,yes
PMC9663740,Figure_3,error_images/gr3.jpg,[],,yes
PMC11404049,Figure_3,error_images/gr3.jpg,[],,yes
PMC8010321,Figure_3,error_images/gr3.jpg,['A punch biopsy from lower portion of his right leg was performed (Fig 3).'],,yes
PMC8717437,Figure_3,error_images/gr3.jpg,['3 Fig. 3The stent was removed 2 weeks later without complications and the patient is awaiting follow up for metabolic screening.'],,yes
PMC6138843,Figure_3,error_images/gr3.jpg,[],,yes
PMC7452279,Figure_3,error_images/gr3.jpg,[],,yes
PMC7701008,Figure_3,error_images/gr3.jpg,[],,yes
PMC7935687,Figure_3,error_images/gr3.jpg,['Eye examination was significant for heterochromia iridis and normal placement of the inner canthi (Fig 3).'],,yes
PMC8058607,Figure_3,error_images/gr3.jpg,[],,yes
PMC7797925,Figure_3,error_images/gr3.jpg,['A punch biopsy of his right shin was performed (Fig 3).'],,yes
PMC4307130,Figure_6,error_images/13014_2014_322_Fig5_HTML.jpg,"['Processing and depiction of other findings such as diagnostic x-ray, pathology and laboratory information are shown in Figures 6 and 7.', 'Figure 6 shows an x-ray finding including a hyperlink to the image data.', '\nFigure 7\nPathology and laboratory findings.']",,yes
PMC4326431,Figure_1,error_images/12957_2014_1873_Fig1_HTML.jpg,"['After extraction, the numbness and pain of the patient worsened; two weeks later, a panoramic radiograph was taken by his dentist, who did not perform any treatment other than to advise follow-up (Figure 1).', 'Figure 1\nInitial panoramic imaging taken by patient’s dentist 2 months ago and showing no apparent lesion.', '\nFigure 2\nPanoramic radiograph taken 1.']",,yes
PMC4326431,Figure_2,error_images/12957_2014_1873_Fig2_HTML.jpg,"['No pathologic findings were detected in other areas (Figure 2).', '\nFigure 3\nCoronal and sagittal cone beam computed tomography (CBCT) images showing erosion in the molar region through the temporomandibular joint (TMJ) area.']",,yes
PMC4326431,Figure_3,error_images/12957_2014_1873_Fig3_HTML.jpg,"['The lesion was also involved in the mandibular canal at the level of the lingula mandibula (Figures 3 and 4).', '\nFigure 4\nAxial cone beam computed tomography (CBCT) images showing erosion in relation to the mandibular nerve on the level of the lingula mandible.']",,yes
PMC4326431,Figure_4,error_images/12957_2014_1873_Fig4_HTML.jpg,['\nFigure 5\nPanoramic reconstructions and 3D cone beam computed tomography (CBCT) images showing severe moth-eaten shaped erosion in relation to the mandibular nerve.'],,yes
PMC4326431,Figure_5,error_images/12957_2014_1873_Fig5_HTML.jpg,"['Panoramic reconstructions and 3D CBCT images showed severe moth-eaten shaped erosion in relation to the mandibular nerve (Figure 5).', '\nOn consideration of the patient anamnesis and radiographs, a biopsy was planned for definitive diagnosis.']",,yes
PMC4301402,Figure_2,error_images/13256_2014_3040_Fig2_HTML.jpg,"['A microscopic examination showed that the mass consisted of a small number of spindle or stellate cells, a small number of vessels, thin collagen fibers and a small number of loose reticular fibers embedded in an abundant myxoid stroma (Figure \n2).', 'Figure 2\nTumor lesion with a small number of spindle or stellate cells, a small number of vessels, and abundant thin collagen fibers (hematoxylin and eosin ×100).', '\nFigure 3\nAt the periphery of the lesion, the skeletal muscle adjacent to the tumor is atrophic.']",,yes
PMC4301402,Figure_4,error_images/13256_2014_3040_Fig4_HTML.jpg,"['\nAn immunohistochemical examination of the mass showed diffuse positivity for vimentin in tumor cells (Figure \n5), focal and weak positivity for CD34 in peripheral regions and was negative for S-100.']",,yes
PMC4301402,Figure_5,error_images/13256_2014_3040_Fig5_HTML.jpg,"['Figure 5\nImmunohistochemical positivity in tumor cells (vimentin ×200).', '\nFigure 6\nImmunohistochemical Ki-67 proliferation index is less than 1% in tumor cells (Ki-67 ×200).']",,yes
PMC4301402,Figure_6,error_images/13256_2014_3040_Fig6_HTML.jpg,"['In the light of pathological data obtained, the mass was concluded to be intramuscular myxoma (Figure \n6).', '\nDiscussionMyxoma is a benign soft tissue tumor of unknown origin\n[1].']",,yes
PMC4349238,Figure_1,error_images/40697_2015_35_Fig1_HTML.jpg,"['If the clot was too close to the anastamosis site (Figure 1), the arterial needle was placed retrograde into the fistula (pointing towards the anastamosis), and the dialysis circuit was put into reverse flow (blood is infused back through the arterial needle and withdrawn from the venous needle).', 'Figure 1\nSchematic illustration of needle placement when clot burden is close to the anastomosis site with dialysis circuit in reverse flow.', '\nSimilarly, if the clot was between the arterial needle insertion site and the venous needle insertion site (Figure 2), the flow will be reversed again but this time both needles will be placed antegrade, away from the anastomosis.']",,yes
PMC10685857,Figure_2,,"['Malignant cells were often dispersed individually without making groups and observed with patches of acute inflammatory cells, erythrocytes, and necrotic debris (Figure 2).', 'Figure 2\nFour Lavage Cytology Samples From Different Patient Groups.']",,yes
PMC3681270,Figure_1,,"['During the evaluation, a chest-abdomen CT scan was performed and a left adrenal mass of 10 × 9 cm was found (Figure 1).', '0-7995573642821450421Figure 1![]() Figure 2![]() Figure 2![]() Figure 4![]() Figure 2![]() Figure 2![]() Figure 3![]() Figure 2![]() Figure 3![]() Figure 4![]() Figure 5![]() Figure 6![]() Figure 7![]() Figure 2![]() Figure 3![]() Figure 4![]() Figure 5![]() Figure 4![]() The patient was discharged with oral lactulose (0.']",,yes
PMC3464901,Figure_1,error_images/1750-1172-7-19-1.jpg,"['Figure 1Cellular localisation and function of rBAT (SLC3A1) and b0,+AT (SLC7A9).']",,yes
PMC3137955,Figure_1,,"['1 cm brain lesion arising from the meninges (Figure 1).', '1StuppRHegiMMasonWEffects of radiotherapy with concomitant and adjuvant temozolamide versus radiotherapy alone on survival in glioblastoma in a randomized phase III study: 5-year analysis of the EORTC-NCIC trialThe Lancet Oncology200910459466192698952WoldenSLAlektiarKMSarcomas across the age spectrumSeminars in Radiation Oncology20102014551199590303KobayashiSHirakawaESasakiMIshikawaMHabaRMeningeal rhabdomyosarcoma: report of a case with cytologic, immunohistologic and ultrastructural studiesActa Cytologica199539342843477623284DropchoEJAllenJCPrimary intracranial Rhabdomyosarcoma: case report and review of the literatureJournal of Neuro-Oncology19875213915033125105LeeJYKimBSPhiJHPrimary meningeal rhabdomyosarcoma associated with chronic subdural effusion: case reportJournal of Neurosurgery201052167171201213656XuFDe Las CasasLEDobbsLJPrimary meningeal rhabdomyosarcoma in a child with hypomelanosis of ItoArchives of Pathology and Laboratory Medicine20001245762765107821657KorithenbergREdelGPalmDMullerKMBrandtMMullerRPPrimary rhabdomyosarcoma of the leptominx: clinical, neuroradiological, and pathological aspectsClinical Neurology and Neurosurgery19848630130560960658SmithMTArmbrustmacherVWViolettTWDiffuse meningeal rhabdomyosarcomaCancer19814782081208671947349CelliPCervoniLMaraglinoCPrimary rhabdomyosarcoma of the brain: observations on a case with clinical and radiological evidence of cureJournal of Neuro-Oncology1998363259267952410410MichalskiJMMezaJBrenemanJCInfluence of radiation therapy parameters on outcome in children treated with radiation therapy for localized parameningeal rhabdomyosarcoma in Intergroup Rhabdomyosarcoma Study Group trials II through IVInternational Journal of Radiation Oncology Biology Physics20045941027103811GuilcherGMTHendsonGGoddardKSteinbokPBondMSuccessful treatment of a child with a primary intracranial rhabdomyosarcoma with chemotherapy and radiation therapyJournal of Neuro-Oncology2008861798217579809Figure 1![]() Figure 2![]() Figure 3![]() Figure 4![]() Figure 5![]() Figure 3![]() Figure 2![]() Figure 3![]() Figure 4![]() Figure 3![]() Figure 8Authors’ original file for figure 3Competing interestsThe authors declare that they have no competing interests.']",,yes
PMC7732322,Figure_1,error_images/aging-12-104012-g001.jpg,"['Representative MTL images are shown in Figure 1.', 'Figure 1Representative medial temporal lobe images, VSRAD Z scores, and amyloid positivity.', 'For all patients, the diagnosis at the time of scanning and the most recent diagnosis by neurology specialists are shown in Supplementary Figure 1.', 'Supplementary MaterialSupplementary Figure 1Figure 2![]() Figure 3![]() Figure 4![]() Figure 6Authors’ original file for figure 1Authors’ original file for figure 2Figure 3Additional file 1: Supplementary Figure 1.']",,yes
PMC7488163,Figure_7,error_images/13024_2020_386_Fig4_HTML.jpg,"[' 7a).', '7a).', 'Fig. 7ContinuedTo confirm these findings, we next examined the recruitment of endogenous TDP-43 to DNA-PKcs foci in cells after etoposide treatment using SR microscopy as above.', '7b).', '7c).', '7c, Supplementary Table 1).', '7b).']",,yes
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