gene_editing.csv

System Question,Question,Option_A,Option_B,Option_C,Option_D,Option_E,Answer,Domain,Difficulty,Type,Task,Reference
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","I'm working on knocking out a gene in human fibroblasts that have already been sorted as GFP+. After editing these cells with CRISPR/Cas, what's the best way to select the successfully edited ones? Is there an additional fluorescent protein that could be integrated with the donor DNA to help with this selection?",Use a different fluorescent protein such as RFP or CFP,Rely on the original GFP marker for selection,Sort cells again based on size,Select edited cells using antibiotic-based selection,Add a GFP target to the donor DNA,d,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>",How can I verify if my LentiCRISPRv2 clones contain the correct oligomer sequence using a digestion method before proceeding to full sequencing?,Perform a restriction digest with BamHI and run on a 1% agarose gel,"Use BsmB1, run a 2% agarose gel for a short duration, and stain with Sybr Safe",Use EcoRI and analyze on a polyacrylamide gel,Perform Sanger sequencing directly without any digestion,Digest with HindIII and visualize using ethidium bromide staining,b,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","When creating a sgRNA library, how can you figure out the right number of cells to include in each glycerol vial to ensure the library's diversity is maintained?",Store at -80°C without any specific cell count requirements,Use a hemocytometer to count cells before freezing,Ensure freezing at least 50 million cells per vial to maintain library diversity,"After scraping off the bacteria, you can spin down and store the pellet at -20C. I would recommend maxiprepping all of the bacteria together to maintain screening coverage.","Freeze any number of cells, as the library diversity is not affected by cell count",d,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","Which sheet should we use for accurate post-sequencing analysis to resolve the discrepancies in gene-sgRNA data from Addgene—Sheet1 or the ""stab format"" sheet?",A new sheet needs to be created by merging data from both Sheet1 and 'stab format' sheet for accurate analysis,"Sheet1, because it contains all the necessary sequence details required for analysis","Neither sheet, as both contain discrepancies that make them unsuitable for accurate analysis",Both sheets are equally valid for analysis as they provide complementary data,"The 'stab format' sheet, because it matches the number of targets 65386 (A) and 58031 (B) as per the protocol",e,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","I've been working with a CRISPRed population of mouse embryonic stem cells using a pair of gRNAs. When I run a PCR with primers flanking the target locus, I can see that deletions are happening. However, after cloning single cells, I only find the wild type band. Shouldn't I be seeing at least one heterozygous deletion? How many colonies should I typically check to find a homozygous deletion? What are your thoughts based on your experiences?",Checking a single colony is usually sufficient as CRISPR is highly efficient.,You should check 10-20 colonies to increase the chance of finding a heterozygous deletion since large deletions might destabilize the cells.,"The process is random, so any number of colonies will yield the same result.","You should check 50-100 colonies because large deletions may reduce the stability of edited cells, leading to fewer detectable deletions after cloning.",It is unusual to see only wild type bands; you should repeat your initial PCR to confirm the deletion.,d,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","I've been working on some screening experiments using SAM, but I'm encountering issues with low lentivirus titers and significant cell death after adding puromycin. Could this problem be specific to the cell line I'm using, and would it help to test other cell lines like HEK293, since similar issues haven’t been noticed in A375 and HUES66 cells?",The cell death is likely due to the SAM system being incompatible with the cell line used,The low lentivirus titer may be due to poor packaging efficiency or instability of the viral particles,The low titer is caused by the lentivirus being improperly stored before use,Puromycin is not suitable for selection in this type of experiment,The problem is likely due to contamination in the viral preparation process,b,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","Does anyone know if there's a lentiviral vector available from Addgene where Cas9 is controlled by a ubiquitin promoter, either constitutively or in a way that's compatible with the Tet system?","No, such vectors are not available at Addgene.","Yes, but only constitutive vectors are available.","Yes, but only Tet-inducible vectors are available.","No, these vectors must be custom-made.","Yes, Addgene offers both constitutive and Tet-inducible Cas9-expressing lentiviral plasmids.",e,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","I'm planning to produce lentiviruses for the Gecko library using the newly published PEI protocol, and I'm wondering if I should change the medium 16 hours after adding the PEI plasmid mixture to the cells. It's not mentioned in the protocol, but we used to do it with regular lentiviral production. Should I stick with leaving the plasmids and PEI mixture in contact with the cells for the full two days until collection?",There is no need to change the medium; harvest after 24h,Changing the medium is unnecessary; harvest after 72h,Leave the plasmids and PEI mixture in contact with the cells for 2 days till collection,Change the medium immediately after adding PEI and harvest 24h later,Change the medium 16h after adding PEI and harvest 48h after the media change,e,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","We've been using desalted ultramers (ssODNs) to create loxP sites in our mice, but after sequencing, we've noticed that every sequence where integration occurs has a deletion of 2 nucleotides in the loxP site. These deletions vary, with some sequences having an AA deletion and others an AT deletion. Could this issue be related to the purity of the ssODNs, and should we have opted for PAGE purification instead?","No, desalted ultramers are always sufficient for accurate gene editing","Yes, PAGE purification can help reduce impurities and improve the accuracy of the sequences","No, the deletions are inherent to the loxP site itself","Yes, but the issue is likely due to the guide RNA, not the ssODNs",,b,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","Has anyone attempted to extract DNA without the vortexing step mentioned in the Zhang lab CRISPR papers, and if so, does omitting this step affect the quality of the DNA extracted?",The standard protocol always requires vortexing for effective DNA extraction,Skipping vortexing leads to incomplete DNA extraction,Vortexing is essential for the thermocycler process to yield high-quality DNA,Omitting the vortex step results in lower quality DNA for PCR assays,The current method without vortexing produces PCR-ready DNA of similar quality,e,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","How can you identify the best gRNA for efficient cutting when delivering RNP complexes into cancer cells, especially if considering using an approach like AsCpf1-Ultra, which has shown promise in human cell lines?",Avoid using electroporation methods,Rely on traditional Cas9 systems for all cell types,Focus exclusively on non-human cell lines,Only use viral delivery systems,Use AsCpf1-Ultra from IDT for human cell lines via RNP delivery,e,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","When designing a 30nt oligo for a CRISPR project using the crRNA:tracrRNA vector (pX260), is it necessary to follow the 'G rule' for transcription initiation, or is this requirement not needed because the direct repeats already start with a G?",Appending a G is optional and does not affect transcription,It is necessary to append a G to ensure proper transcription,"The G rule only applies to the tracrRNA, not the oligo",It is not necessary because the direct repeats start with a G,The G rule is irrelevant when using a crRNA:tracrRNA vector,d,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>",Has anyone had any experience or knowledge about using the CRISPR/Cas9 gesicle system as an alternative strategy for gene editing in hard-to-transfect cell lines?,It involves the use of electrical pulses to introduce CRISPR/Cas9 into cells,It utilizes bacteriophage vectors for delivery to eukaryotic cells,It is a chemical-based method that uses liposomes to encapsulate CRISPR/Cas9 complexes,"It is a method of delivering CRISPR/Cas9 components via cell-derived vesicles, potentially improving delivery efficiency in hard-to-transfect cell lines",,d,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","In a discussion on genome engineering using CRISPR/Cas systems, someone asked: Is it feasible to design guide RNA for CRISPR/Cpf1, specifically for targeting intergenic spacers, which are a subset of noncoding DNA?","Yes, as long as the intergenic spacer is part of a known regulatory element.","Yes, it is possible as long as you can find a PAM, which should be easier for Cpf1 in noncoding regions that are more AT rich.","No, gRNA design is restricted to exonic regions in the genome.","No, it is not possible because gRNA can only be designed for coding regions.","Yes, but only if the intergenic spacer contains repetitive sequences.",b,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","Do you have any insights on whether to choose the sense or antisense ssDNA strand for modifying coding versus non-coding sequences, regardless of the guide sequence? Also, should this choice be prioritized over the previously discussed consideration of whether the strand is the same or opposite to the sgRNA, given the general efficiency observed with antisense oligo donors?","The antisense strand is generally more efficient, especially in coding sequences",There is no significant difference between sense and antisense strands,Both strands are equally efficient in all cases,The sense strand should be prioritized for non-coding sequences,The choice of strand is more critical than the 'relative same/opposite strand to sgRNA',a,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","I have a quick question. According to the cloning protocol, we're supposed to use the U6-forward sequencing primer (5'GAGGGCCTATTTCCCATGATTC-3') for plasmid sequencing. Should we use this primer for all guide sequences, or does it vary depending on the specific sequence?",It depends on the length of the guide RNA.,Only if the guide sequence is longer than 20 nucleotides.,Only for guide sequences targeting non-coding regions.,"Yes, the U6 forward Sanger sequencing primer can be used for all guides.","No, you need a different primer for each guide sequence.",d,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","In the context of our discussion on genome engineering with CRISPR/Cas systems, could you tell me where I can find the online tool that helps identify suitable sgRNA pairs for directed nuclease applications?",In the supplementary materials of a recent Nature paper on CRISPR/Cas systems,On the online web tool developed for identifying sgRNA combinations for double nicking applications,By using a proprietary software available at most biotech companies,Through a subscription-based service offered by Addgene,In the CRISPR Cas9 handbook provided by the Broad Institute,b,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","Is there a genome-wide CRISPR knock-out library available or in development that doesn't rely on lentivirus, or is there a way to use a lentiviral library as a direct plasmid instead?",A lentivirus library that isn't packaged yet and can be directly used as plasmids,A library based on electroporation-compatible vectors,A lentivirus library that is packaged and ready to use,An adenoviral vector library for CRISPR screens,,a,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>",I'm just curious about your choice against using a lentiviral plasmid for gene editing. Is it related to concerns about efficiency or its applications in genome engineering?,Lentiviral plasmids are not compatible with all cell types,Lentiviral vectors have a higher risk of insertional mutagenesis,Lentiviral plasmids are more expensive to produce,"Lentiviral plasmids can integrate the gene of interest into the host genome, leading to stable expression",Lentiviral plasmids are less efficient for gene delivery compared to other vectors,d,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench
"You are an expert in CRISPR gene editing technologies. Your task is to select the most appropriate answer from the given multiple choice options based on the user's question.

Given Information:
- Question: {{QUESTION}}

Available Answer Choices:
a. {{OPTION_A}}
b. {{OPTION_B}}
c. {{OPTION_C}}
d. {{OPTION_D}}
e. {{OPTION_E}}

Requirements:
- Analyze the question and experimental context
- Evaluate each option based on current CRISPR best practices
- Select the most scientifically accurate and practical answer
- Provide only a single letter (a-e) as your answer
- Wrap your answer in the corresponding tag

Output Format:
<ANSWER>[single letter: a, b, c, d, or e]</ANSWER>","What are the reasons behind the varying effects of using the CRISPR/Cas9 system for gene knockout in human primary cells, depending on the individual subject?",Because the environmental conditions are the same for all subjects,Because CRISPR/Cas9 does not work in human cells,Because the primary cells from different subjects have identical growth rates,Because the CRISPR/Cas9 system is universally efficient across all subjects,Because different subjects have genetic variations that affect gene knockout efficiency,e,Biology,medium,Advance Reasoning,Gene editing,Genome-Bench