Challenges Of Using Homomorphic Encryption In Machine Learning Soumia Zohra El Mestari SnT, University of Luxembourg Introduction Homomorphic Encryption (HE) is a form of public key encryption that allows evaluating a function on encrypted data without the need to decrypt [3, 1]. The adoption of HE in Machine Learning (ML) contexts faces many challenges, namely: • Non support for non linear functions • Noise growth • Secret key recovery attacks Non support for non linear functions Fully Homomorphic Encryption (FHE) schemes so far only support addition and multiplication hence machine learning activation functions are then not supported for direct FHE evaluation. There are three commonly used methods to support non-linear functions: • use a polynomial substitution • pre-compute function for discrete values and store these values in look-up tables • use low-degree polynomials to approximate the non-linear functions All these methods are approximations so they cause a precision loss in the case of inference and additional convergence time if we train on homomorphically encrypted data. Noise growth Approximate FHE schemes like CKKS [1] decrypt to an approximated value of the original message. Figure: Approximate Homomorphic encryption evaluation The sources of noise are caused by the encoding and the noise growth after each operation with the multiplication having the most noise growth scale. The bootstrapping operation despite being one of the solutions to reduce the noise in ciphers is computationally expensive and hence the homomorphic evaluation of deep neural networks for example can face large computation overheads. Secret key discovery attacks When using approximate numbers schemes like CKKS [1], a passive attacker having access to ciphers and their decrypted versions can recover the secret key by analysing the noise growth in ciphers; in fact, the results are approximated, and the IND-CPA security does not oﬀer suﬃcient protection. Figure: Secret key recovery attack on CKKs [1] Such a devastating attack can be mitigated by injecting an additional Gaussian noise during the decryption which will make the precision decrease. Conclusion The adoption of HE in ML systems is promising, multiple attempts ( such as CryptoNets [2] ) started emerging HE inference as attempts to oﬀer conﬁdentiality level of privacy to machine learning tools. References [1] J. H. Cheon, A. Kim, M. Kim, and Y. Song. Homomorphic encryption for arithmetic of approximate numbers. In International Conference on the Theory and Application of Cryptology and Information Security, pages 409–437. Springer, 2017. [2] R. Gilad-Bachrach, N. Dowlin, K. Laine, K. Lauter, M. Naehrig, and J. Wernsing. Cryptonets: Applying neural networks to encrypted data with high throughput and accuracy. In International conference on machine learning, pages 201–210. PMLR, 2016. [3] P. Martins, L. Sousa, and A. Mariano. A survey on fully homomorphic encryption: An engineering perspective. ACM Computing Surveys (CSUR), 50(6):1–33, 2017. Acknowledgements This work has been funded by the European Union’s Horizon 2020 Innovative Training Networks, Legality Attentive Data Scientists (LEADS) under Grant Agreement ID 956562 Contact information • Email: soumia.elmestari@uni.lu 1/1


Related Literature [1] Maria Roussou and Akrivi Katifori. 2018. Flow, Staging, Wayfinding, Personalization: Evaluating User Experience with Mobile Museum Narratives. Multimodal Technol. Interact. 2, 2 (June 2018), 32. DOI:https://doi.org/10.3390/MTI2020032 [2] Akrivi Katifori, Maria Roussou, Irene Kaklopoulou, Katerina Servi. 2019. Mobile interactive storytelling in the Athens Ancient Agora: exploring the right balance between the Site and the App, XVII. Culture and Computer Science, May 23-24, 2019, Konzerthaus Berlin. [3] Akrivi Katifori, Sara Perry, Maria Vayanou, Angeliki Antoniou, Ioannis-Panagiotis Ioannidis, Sierra McKinney, Angeliki Chrysanthi, and Yannis Ioannidis. 2020. “Let Them Talk!”: Exploring Guided Group Interaction in Digital Storytelling Experiences. J. Comput. Cult. Herit. 13, 3 (August 2020), 1–30. DOI:https://doi.org/10.1145/3382773 Conclusion The approaches followed within the three projects, αlthough they differ from one to another, because of variations in archaeologists’ needs, the scope of the experience, and the site’s characteristics, all of them draw inspiration from the monuments, the historical events, and the people to deliver pertinent information as effortlessly as possible. The aim is to engage the user emotionally with the people who lived in ancient Eleusis, Arta and Epidaurus, to understand their needs, desires, habits, and different perspectives, and to transform his or her connection to the history and the archaeological site from the level of simple knowledge into a deeper, multilevel, and rich experience. A multi-layered experience through space and time in the archaeological sites of Arta In Voeska, a multi-layered experience has been adopted that runs through space and time. Considering the uniqueness of Arta and the fact that it has three charming historical faces, a flexible core idea was chosen, which finds application in different periods and offers the ability to link objects to spaces. The focus of the idea is a scribe, who undertakes to write a series of letters, which are ordered by his clients. The Epistles cover all time periods and involve a wide range of people: men, women, aristocrats, peasants, craftsmen, artists, kings. The persons who send and receive the letters could be fictitious or real or both. The main advantage of this idea is that it can include a variety of themes, so as to create a "mosaic" in which each piece is independent, but all together create a broader and timeless image of Arta, while touring the city. Moreover, it effortlessly links fiction to specific objects and sites, and in fact, each Epistle can link more than one object to more than one site. Utilizing the main components of fiction, the selected approach activates historical empathy. A linear digital storytelling experience connecting the past with the present of the underwater antiquities of Epidaurus In Periplous, α journey aboard a boat with transparent bottom is accompanied by an automated guided tour, where the digital experience complements the physical one in real time as the visitors float above the underwater antiquities of Epidaurus. The boat follows predetermined routes and stops mid-sea while providing descriptions and narrations. Α linear approach for the digital storytelling experience is adopted, connecting the past with the present. Within this context, visitors will be able to discover ‘hidden’ information in a series of points of interest, called "Snapshots”, including pre- recorded narrations or conversations connected to the archeological findings and representing everyday life in the ancient times. The participants in the dialogues are either ‘ancient people’ who lived in Epidaurus, as represented through fictional dialogues, or the real archaeologists, who have undertaken the excavation and the conservation of the specific archeological site. An interactive digital storytelling experience in ancient Eleusis In myEleusis, an interactive digital experience unfolds through


The Tonal Comparative Method: Tracing Sound Change in Lexical Tone Rikker Dockum The Comparative Method We can deconstruct the Comparative Method to first principles and rebuild it in a way that allows for tonal evidence What do sound change rules like this pick out from the language? *p > b / V_V o The object of comparison in the CM is lexical subsets that pattern together in a particular conditioning environment o When viewed in this way, lexical tones with their segmental origins are a natural fit Building the missing evidence Expanding the Comparative Method SUMMARY: Linking tones to their segmental origins helps us to use tonal evidence in language classification and reconstruction in a methodologically sound way Lexical tone and the Comparative Method The Kra-Dai languages o ~100 languages o ~100 million speakers (Edmondson & Solnit 2008) Tai is the most populous branch o ~80 million speakers o Thailand 65+ million o China 15+ million o Laos 4+ million o Myanmar 3+ million o And more in India, Vietnam, Cambodia, Malaysia o The Comparative Method has long been the main tool of linguists for figuring out what past stages of a language sounded like without direct evidence o Systematic comparison of cognate words in related languages o Working backwards from the present and inferring sound changes in daughter languages o The main tool of linguists for determining genetic relationships between languages, and reconstructing common ancestors (i.e. proto-languages) The segmental origins of tone o Lexical tone, the use of pitch to encode word meanings, has often been thought to be unusable with the Comparative Method (e.g. Meillet 1948, Campbell 2003) o The origin of tone compensates for loss of segmental contrasts o But after initial tonogenesis, tones vary and change in ways still poorly understood Why do tones change? o Sound change is constantly happening o Both conditioned changes and random drift, just as with segments “[past use of Tai tonal evidence] is not consistent with the shared-innovation method used in subgrouping, because many tonal changes may not in fact be shared innovations … A subgrouping proposal for Southwestern Tai should primarily use as criteria consonantal and vocalic changes that can be shown empirically to have occurred relatively early.” (Pittayaporn 2013:306) What is missing? o In the traditional Comparative Method we have a large body of accumulated knowledge, the received wisdom of what kinds of sound changes are more or less likely than others o Key to the scientific validity of the method is having generally reproducible principles for distinguishing retentions and innovations from chance resemblance (Weiss 2015) o There is no body of received wisdom for sound change in lexical tone, no accumulated knowledge of which sound changes are more or less likely “…prosodic change seems fully tractable in terms of analytical methods ... time-tried for other aspects of phonological change … on the other hand, there is as yet so much to be learned … the present lack of data may enforce, at a minimum … one or two generations of waiting until two or more richly described contiguous points in time are available for comparison” (Janda and Joseph 2003:117) Tone in the Tai languages Proto-Tai had three tones on open syllables/sonorant codas: A B C *hmaA ‘dog’ *hmaB ‘to soak’ *hmaC ‘to grow, rise’ The 3-tone system of Proto-Tai split into more tones based on the laryngeal configuration of onset consonants: Each daughter language carves up the ‘tone box’ (Gedney 1972) differently. For example, here is Standard Thai (each color represents a surface tone): A B C Voiceless w/ friction A1 B1 C1 *pʰ, *tʰ, *s, *ʰm, etc. Voiceless unaspirated A2 B2 C2 *p, *t, *k, etc. Glottalized A3 B3 C3 *ʔ, *ʔb, *ʔj, etc. Voiced A4 B4 C4 *b, *m, *l, *z, etc. A B C Voiceless w/ friction khaː24 ‘leg’ khaː22 ‘galangal’ khaː41 ‘servant’ *pʰ, *tʰ, *s, *ʰm, etc. Voiceless unaspirated ka33 ‘crow’ kaj22 ‘chicken’ klaː41 ‘seedling’ *p, *t, *k,


Constraining Gas Disk Structure in MY Lupi with UV and Submillimeter Models Nicole Arulanantham1, Paolo Cazzoletti, Simon Bruderer, Anna Miotello, Ewine van Dishoeck, Kevin France, Keri Hoadley Contact: narulanantham@stsci.edu Giacconi Fellow, Space Telescope Science Institute This project has been supported by a NASA Earth and Space Science Fellowship (NESSF) and HST-GO program 14469 (co-PIs: C.F. Manara, P.C. Schneider). Physical-chemical models generate detailed maps of carbon, nitrogen, oxygen, and hydrogen bearing molecules in protoplanetary disks. However, the vertical disk structure in these models is degenerate with the amount of UV flux reaching the gas. Flat, strongly irradiated disk models and flared, weakly irradiated systems produce similar integrated line fluxes, which makes it difficult to examine UV sensitive photochemical pathways at disk radii where submillimeter gas emission cannot yet be spatially resolved. How does the multi-wavelength modeling approach work? The 2-D distributions of vibrationally excited molecular hydrogen from each DALI model are irradiated with LyA photons, which pump the gas into excited electronic states. The resulting fluorescent emission lines are compared directly to observed UV features in HST-COS spectra of MY Lupi, showing that the H2 lines are best fit by a flared disk model with strong FUV irradiation and a high gas mass. Can multi-wavelength spectroscopy break the degeneracy between vertical disk structure and UV irradiation of the gas? We investigate whether ultraviolet emission lines from fluorescent molecular hydrogen can be used to break this degeneracy, by using the Dust and LInes (DALI) code (Bruderer et al. 2013) to model CN emission in the disk around MY Lupi. We have chosen MY Lupi as a prototype, since its flaring angle has been directly measured from scattered light observations (see below). How can we study the composition and distribution of gas in regions of protoplanetary disks where rocky planets form? Flat disk, high UV Flared disk, low UV z/r r [au] r [au] H-band scattered light image of MY Lup (Avenhaus et al. 2018), which constrained the disk flaring angle (ψ = 0.21 ± 0.03) z/r N + H⇤ 2 −! NH + H C+ + NH −! CN+ + H CN+ + H −! CN + H+ Visser et al. 2017, Cazzoletti et al. 2018 Pumped by Lyα to produce UV- H2 emission Outstanding question: do any disks show UV-H2 emission lines that look like the flat disk model? We conclude that molecular emission lines from UV spectra can provide important constraints for physical- chemical models of gas disks. However, further observations are required to determine whether flatter disks produce UV-fluorescent H2 emission that looks like the models.


The exclusion criteria were: Previously diagnosed endometriosis, uterine leiomyomas, previous multiple surgeries, endocrinological disorders, and severe male factor infertility. Number Patient IUI İVF Pregnancy Term-Live Birth Clomiphene-1 Clomiphene-2 Clomiphene-3 Clomiphene-4 Letrozole-1 Letrozole-2 Letrozole-3 Follitropine-alfa 1 Follitropine-alfa 2 50 40 30 20 10 0 STUDY DESIGN The inclusion criteria were: Age between 20-39 years, basal FSH level < 10 mIU/ml, and body mass index<35 kg/m2. Patients who meet these criteria were included In the study program. One hundred thirty-six subfertile women who applied to the Obstetric and Gynecology Clinic at Bakırköy Sadikonuk Education and Research Hospital were allocated. Between February 2020 to January 2022, We accessed patient files from archive records. The patients were divided into two groups; use of clomiphene citrate (n=107) and letrozole (n=19). We gave follitropine alfa to some of the patients (n=19). LIVE PREGNANCY RATE AFTER OVULATION INDUCTION PROTOCOLS IN SECONDARY INFERTILE PATIENTS AUTHORS Mustafa Cengiz Dura1 Hilal Aktürk1 Murat Ekin2 AFFILIATIONS Bakırköy Sadikonuk 1Education and Research Hospital INTRODUCTION Ovulation induction protocols, including Clomiphene Citrate(Serm), GnRH analogs (GnRH agonist and antagonist), and Letrozole(aromatase inhibitor), are known and enumerated. Our study is cross-sectional and retrospective and obtained from the patients who applied to the infertility outpatient clinic and the hospital database between February 2020 to January 2022. RESULTS/FINDINGS OBJECTIVE İn this study, we aimed to investigate live pregnancy rates after variable ovulation induction protocols in secondary infertile patients. ANALYSIS CONCLUSION Our data found no significant difference in comparing ovulation induction protocols in live pregnancy at term. PARAGRAPH METNİNİZ It results in term pregnancy after ovulation induction; in secondary infertile patients. A significant relationship exists between previous pregnancies and live- term birth rates (p<0.05). At the same time, there is no significant relationship in live pregnancy after using clomiphene citrate, letrozole, and follitropine-alfa for ovulation induction. In our research, we use one after another Clomiphene Citrate first attempt (n=48) 38.1%, Clomiphene Citrate second attempt (n=42) 33.3%, Clomiphene Citrate third attempt (n=14) 11.1%, and Clomiphene Citrate fourth attempt (n=3) 2.4% rates. In another group, they take after another Letrozole first attempt (n=11) 8.7%, Letrozole second attempt (n=5) 4.0%, and Letrozole third attempt (n=3) %2.4 Nineteen patients take Follitropine-alfa .19 out of 16 patients take Letrozole after that; they take Follitropine-alfa when there is no adequate response; simultaneously, 107 out of 3 patients take Clomiphene Citrate after that; they take Follitropine-alfa. There was no significant difference between the groups in measurements after term delivery, marriage duration, infertility period, anti-mullerian hormone, follicular stimulating hormone, and spermiogram evaluation (p>0.05). TSRM 2022 10.ÜREME SAĞLIĞI VE İNFERTİLİTE KONGRESİ 10-13.KASIM 2022 GİRNE/KKTC Chart-1:Comparison of ovulation induction protocols in terms of live pregnancy at term. Figure-1:Comparison of ovulation induction protocols Total Number of Patient Number of secondary infertile patients Live pregnancy at term 136 126 29


Data-driven model of striatal external input Ilaria Carannante1, Yvonne Johansson2, Johannes Hjorth1, Gilad Silberberg 2, Jeanette Hellgren Kotaleski1,2 1 KTH Royal Institute of Technology, Department of Computational Science and Technology, Stockholm, Sweden. 2 Karolinska Institutet, Department of Neuroscience, Stockholm, Sweden. The basal ganglia play an important role in a variety of functions including decision making and action selection mainly based on input from cortex, thalamus and the dopamine system. Striatum is the input stage of the basal ganglia. Here we present a new approach to model the postsynaptic currents mediated by NMDA or AMPA receptors which allows a more accurate description of them. Then we model the extrinsic inputs from cortex and thalamus to the different populations of striatal neurons (striatal projection neurons (dSPNs and iSPNs); cholinergic (ChIN), fast-spiking (FS), and low-threshold spiking (LTS) interneurons). We present a data-driven model of the postsynaptic currents mediated by AMPA or NMDA (average currents are shown in Fig2). We describe the dynamics using three exponentials: one for the rising and two for the decay phases. The use of three exponentials (instead of the generally used two) allows a more accurate description of the currents without compromising the performace of the models. A comparison between the different current models is shown in Fig3. We introduce a classification of the synaptic traces for each input region and each cell type. Traces belonging to the same class are normalized and the average trace is extracted. An example of this process is shown in Fig4. We simulate the extrinsic inputs by placing and stimulating the glutamatergic synapses on multicompartmental neuron models. These are based on detailed reconstructed morphology and include ion channels models according to RNA-seq data. An example of LTS models is shown in Fig5. The models are able to capture both subthreshold and suprathreshold current-voltage responses. [1] Hjorth, J. J., Kozlov, A., Carannante, I., Nylén, J. F., Lindroos, R., Johansson, Y., ... & Grillner, S. (2020). The microcircuits of striatum in silico. Proceedings of the National Academy of Sciences, 117(17), 9554-9565. [2] Johansson, Y., & Silberberg, G. (2020). The functional organization of cortical and thalamic inputs onto five types of striatal neurons is determined by source and target cell identities. Cell reports, 30(4), 1178-1194. [3] Uziel, A., & Markram, H. (2000). Synchrony Generation in Recurrent Networks with Frequency-Dependent Synapses. Journal of Neuroscience, 20(1), RC50-RC50. Funding: The study was supported by the Swedish research council (VR-M-2020-01652); Swedish e-Science Research Centre (SeRC); EU/Horizon 2020 no. 945539 (HBP SGA3); KTH Digital Futures. The simulations were enabled by resources provided by the Swedish National Infrastructure for Computing (SNIC) at PDC KTH partially funded by the Swedish Research Council through grant agreement no. 2018-05973. We acknowledge the use of Fenix Infrastructure resources, which are partially funded from the European Union's Horizon 2020 research and innovation programme through the ICEI project under the grant agreement No. 800858. Conclusions and future works Synapses are critical when investigating microcircuit functions and dynamics, therefore it is necessary to have a computationally efficient and accurate framework to model them. Here we are improving such a pipeline using morphologically detailed neurons. Our method is generic and hence applicable to any brain region. We are working on the integration of these models into large scale networks [1] as part of our ongoing effort to create detailed simulations of the entire striatum. We optimise the parameters of a Tsodyks-Markram model [3] to match and reproduce the different behaviours observed experimentally [2]. A comparison between the different synapse models (based on the different current models of NMDA and AMPA


A Novel Approach to Combat Antibiotic Resistance in Nosocomial Infections: Exploring the Synergistic Potential of Berberine and Glycerol Aziza Abugaliyeva, Annette Vincent, and Simon Faulkner Biological Sciences Program, Carnegie Mellon University Qatar Abstract Introduction Figure 1. Calibration curve for Berberine Chloride (BC) in Methanol. 10-50 ug/mL dilutions of BC were prepared using methanol. The absorbance was measured at 429 nm using Agilent 8453 UV-Vis spectrophotometer. Conclusions and Future Work References [1] Wojtyczka, R., Dziedzic, A., Kępa, M., Kubina, R., Kabała-Dzik, A., Mularz, T., & Idzik, D. (2014). Berberine Enhances the Antibacterial Activity of Selected Antibiotics against Coagulase-Negative Staphylococcus Strains in Vitro. Molecules, 19(5), 6583–6596 [2] Nguyen, H. T., Pham, T. N., Le, A. T., Thuy, N. T., Huy, T. Q., & Nguyen, T. T. T. (2022). Antibacterial activity of a berberine nanoformulation. Beilstein journal of nanotechnology, 13, 641–652. [3] AlMulla, M. (2023). Solubility Verification of Berberine Chloride (BC) in Natural Deep Eutectic Solvents (NADES) for the Development of Novel Antimicrobial Agents, CMUQ. [4] Battu, S. K., Repka, M. A., Maddineni, S., Chittiboyina, A. G., Avery, M. A., & Majumdar, S. (2010). Physicochemical characterization of berberine chloride: a perspective in the development of a solution dosage form for oral delivery. AAPS PharmSciTech, 11(3), 1466– 1475. • BC is an isoquinoline alkaloid extracted from plants. It exhibits weak antimicrobial activity against E. coli by itself [1]. • Early studies proposed certain approaches such as synergistic effects with other antimicrobial drugs to increase the effectiveness of BC [2]. • BC has limited solubility in water (1.96 ± 0.11 mg/mL) but it was found to readily dissolve in glycerol which stabilizes bacterial growth and prevents any damage to the cell membrane [3] [4]. Acknowledgments I want to express my immense gratitude to Dr. Simon Faulkner, Dr. Annette Vincent, and Ms. Maria Navarro for their guidance, assistance, and support throughout this research. Methods Gly 5% Gly 10% Gly 15% Gly LB+ LB+ LB+ Water LB Nosocomial infections, acquired during hospital treatment, are a significant challenge in healthcare settings. They are often caused by pathogens like E. coli, which have multiple virulence genes, making traditional treatments less effective due to antibiotic resistance. Berberine chloride (BC), a compound derived from the plant genus Berberis comprised of ~450-500 species, has shown antimicrobial activity against E. coli but its efficacy is limited when used alone. Combining BC with other antibiotics enhances its antimicrobial properties synergistically. Our research aims to improve BC's solubility in glycerol, enabling better dissolution in aqueous media without toxic co-solvents using Minimum inhibitory concentration (MIC) assay and solubility tests. This enhancement will optimize BC's therapeutic potential against nosocomial infections, providing valuable insights for more efficient treatment strategies. Sample Average rate (abs/hrs) STDEV control 0.309 0.006 (n=4) 5% Gly 0.259* 0.031 (n=4) 10% Gly 0.152 0.006 (n=2) 15% Gly 0.023# 0.015 (n=3) Table 1. Average growth rate of E. coli C600. Table 2. Solubility of BC in Gly, Water, and LB. y = 0.0147x + 0.0036 R2 = 0.9997 Results Figure 2. MIC assay to determine the effect of varying concentrations of Glycerol on E. coli C600. 0-70% glycerol was mixed with 10-3 diluted bacteria in a 96-well microplate incubated at 600 nm and 30OC for 20 hours in a Varioskan Lux machine. Solvent mass (mg) of BC added to 5 mL solvent Concentration of dissolved BC (mg/mL) Gly 101.6 14.45 Water 100.5 1.87 LB 100.7 0.06 Solvent mass (mg) of BC added to 5 mL solvent Concentration of dissolved BC (mg/mL) 5% Gly + LB 100.8 0.12 10% Gly + LB 100.2 0.11 15% Gly + LB 100.1 0.19 Table 3. Solubility of BC in 5, 10, and 15% Gly including LB. The solubility of BC in 5, 10, and 15% glycerol-LB mixture


BETWEEN UTOPIA AND DYSTOPIA LIES THE LAND OF KNOWLEDGE OR HOW TO STIMULATE SOCIAL DISCOURSE BASED ON FACTS RATHER THAN FEAR Dr. Luiza Bengtsson Max-Delbrück-Centrum für Molekulare Medizin in der Helmholtz-Gemeinschaft luiza.bengtsson@mdc-berlin.de @bengtza @OOSP_ORIONPod PUBLIC DIALOG ON GENOME EDITING IN UK, GERMANY, SWEDEN & CZECHIA • 3-MONTH RESIDENCY IN MDC LABS, REFINING CONCEPT, PROOF OF CONCEPT EXPERIMENTS • ARTIST MEETS SCIENTISTS: MODERATED CLASH OF CULTURES • OPEN CALL: WINNER EMILIA TIKKA • ORION OPEN SCIENCE PROJECT: CO-CREATION EXPERIMENTS • EXHIBITIONS LESSONS LEARNED!


U.S. Environmental Protection Agency Office of Research and Development EPA www.epa.gov Thomas 2019 Bryant A. Chambers and Imran Shah Center for Computational Toxicology and Exposure, US EPA Constructing Unique Stress Signatures Identifying best performing stress signatures Stress signature activity within LINCS test set Conclusions and future directions Bryant A. Chambers l chambers.bryant@epa.gov l 919.541.4268 Finding a test set within the Library of Integrated Network- Based Cellular Signatures (LINCS) SR Pathway Accuracy in 1st Accuracy by 2nd Accuracy by 3rd DDR-400 75% 100% 100% UPR-050 100% 100% 100% HSR-GO 57% 100% 100% HPX-WINT 100% 100% 100% MTL – 200 33% 33% 67% OSR – 200 25% 50% 50% Mean Accuracy 72% 88% 91% Background and Hypothesis Key Conclusions • SRP can be accurately identified using gene signatures in transcriptomic data. • Distinct signals arise from well known chemical agents. • Consensus SRP signatures perform better than published signatures. • Consensus SRP signatures activity identified in LINCS data test set. Future Directions • Concentration-response analysis to estimate benchmark concentrations (BMC) • The lack of available methods to test non-specific chemical points of departure can be expanded to include a new approach method derived from general stress response transcriptomic assays. • Tissue and cell specificity • Expression of stress response systems is partially dependent on cell and tissue type; as such, a deeper understanding of tissue dependency must be achieved. SRP Abbreviation Reference Chemical DNA Damage Response DDR Benzo(a)pyrene Glycinamide Lasicoarpine Methylmethanesulfonate Unfolded Protein Response UPR Brefeldin A Thapsigargin Tunicamycin Heat Shock Response HSR Geldanamycin Heat with recovery Radicicol Response to Hypoxia HPX Oxygen VU-0418946-1 VU-0418946-2 Metal Response MTL Silver Nitrate Zinc Oxidative Stress Response OSR Hydrogen peroxide Tert-butyl hydrogen peroxide A) Activity of SRPs in training data set B) Performance of SRP signatures against randomized null C) Performance of SRP signatures against published signatures Accuracy of Signatures in training data set Highly gene-overlapped published signatures 𝑮𝑮𝑮𝑮𝑮𝑮𝑮𝑮𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨 𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨𝑨 Unique consensus gene space Consensus Signature Set Construction • Identified 48 relevant published signatures by inclusion of ~ key genes • Use “Wisdom of the Crowd” to score gene inclusion • Frequency based (f): 𝑓𝑓𝑖𝑖𝑖𝑖𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔−𝑓𝑓𝑜𝑜𝑜𝑜𝑜𝑜𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔 𝑓𝑓𝑖𝑖𝑖𝑖𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔+ 𝑓𝑓𝑜𝑜𝑜𝑜𝑜𝑜𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔𝑔 Reference Chemicals • 49 reference chemicals were identified in the literature • 2000 GSEs found in the Gene Expression Omnibus matching • Only 35 GSEs proved sufficiently annotated DDR UPR HSR HXP MTL OSR Highly Probable Library of Integrated Network-Based Cellular Signatures (LINCS) • Searched all LINCS chemicals in pub med against stress systems • 7 terms (e.g., ‘DNA damage’, ‘er stress’, unfolded protein response’) • Totaled 32,679 searches • Pairwise mutual information (PMI) score calculated for each chemical • 𝑃𝑃𝑃𝑃𝑃𝑃𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐, 𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠= log 𝐹𝐹(𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝑐,𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠) 𝐹𝐹𝑐𝑐𝑐𝑐𝑐𝑐𝑐𝐹𝐹(𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠𝑠) • Filtered to chemical with PMI > 1 and references >200 yielding a subset of 90 perturbagens • Pulled first 500 abstracts for each match and validated 68 high probability chemicals by review • Transcriptomic Dataset includes: • 11000 Profiles • 81 cell types Concentration response for HSR chemical geldanamycin Activity of SRP consensus signatures was evaluated in 68 “highly probable” chemicals in the LINCs database. Cell type aggregated data indicates broad applicability • 100% of chemicals showed activity in at least one cell line • 92% of HSR and 88% of UPR in cell aggregated data • 30/68 chemical have concentration response • Tissue and cell type is IMPORTANT DDR UPR HSR HPX MTL OSR References Ankley (2010) Environmental Toxicology and Chemistry, 29: 730-741 Simmons (2009)Toxicological Sciences 111(2,) 202–225 Judson (20


Localizing Stellar Activity on Low Mass Stars with the Transiting Exoplanet Survey Satellite 2. Sample Demographics 1. Stellar Activity Julie Moquin1,2, Jordan Ealy1,3, Joshua Schlieder1 1Goddard Space Flight Center, 2Rice University, 3University of Maryland College Park Fig 1. The above ﬁgure depicts how a ﬂare is created through magnetic ﬁeld reconnection. ●Low-mass stars often have high ﬂaring rates ●Caused by a sudden magnetic reconnection ●Usually associated with star spots [Ikuta et al. 2023] ●Spot modulation is caused by a decrease in optical ﬂux from an increase in star spot coverage. ●BANYAN Moving Group Stars [Gagné et al. 2018] ○Stars from β Pictoris Moving Group (βPMG), AB Doradus Moving Group (ABDMG), Tucana-Horologium Association (THA), Columba Association (COL), Carina Moving Group (CAR) ●Sample optimization removed the ﬁeld stars as they do not have detectable spot modulation. ●We used Lightkurve [Lightkurve Collaboration 2018] to examine the light curves for possible contamination. Credit: NASA/ESA/G. Baco 3. Data Analysis Phase Folding the Light Curves Fig 5. Phase-folded light curve with an oﬀset. Using an oﬀset makes it easier to see where the ﬂares are located in the phase-folded graph. Fig 4. (a) a TESS light curve. (b) The same light curve, but phase-folded. ●To phase-fold a light curve, the light curve is folded based on the star's rotation rate which we estimate using a Lomb-Scargle periodogram ●Phase-folding aids with visualizing in which area of the period the ﬂares are located Localizing Stellar Activity on Low-Mass Stars with the Transiting Exoplanet Survey Satellite Julie Moquin Jordan Ealy Joshua Schlieder Credit: NASA/ESA/G. Bacon (STScI) βPMG (~24 Myr§) ABDMG (~149 Myr§) THA (~45 Myr§) COL (~42 Myr§) CAR (~45 Myr§) 8 4 4 11 1 33.7% 17.1% 27.1% 1.0% 21.1% Fig 3. The outer donut chart depicts the percentage of light curves used from each moving group. The inner donut chart depicts the number of sources used from each moving group. The color blocks are diﬀerent shapes as some stars had multiple sectors of observation. The sectors were treated individually to maintain observation integrity. ●Trends in ﬂare rates and spot modulation can provide a means to “localize” on the rotation phase where ﬂares occur more frequently and whether they are associated with active regions (ie star spots). We selected our sample to include young stars. Fig 2. Spot modulation is seen in light curves as the dips in ﬂux. For young stars, other sources of variability can cause aperiodic brightness changes and lead to complex, non-sinusoidal morphologies. The zoom in shows the morphology of a ﬂare and the spot modulation. Future Work Localizing the Flares ●Inﬂection Point Sectioning ●Rigorous Statistics on Flare Distribution Results ●Continuum localization ●To validate our methods, we did an initial survey on 44 light curves with consistent, sinusoidal spot modulation. We plotted the start location of ﬂares along the rotation phase. The phase-folded light curves were co-aligned in phase space using their minima. We used AltaiPony [Ilin et al. 2021] to detect the ﬂares on all the light curves. A ﬂare is detected if there is enough data 3σ above the midline. The midline is found by detrending the light curve, which removes the periodic signal from the light curve. Identifying the Flares Fig 6. The ﬂares are highlighted in blue. This is useful for making sure that all ﬂares are identiﬁed properly and allows for localization analysis by eye. Scan me for abstract, contact information, and references! Fig 7. All panels are aligned in phase and colored by eﬀective temperature of the ﬂaring star. (a) Phase-folded light curves. Oﬀset peaks show varied spot coverage. (b) Distribution of the ﬂare start times. (c) Cumulative histogram of the ﬂare density with a kernel density estimate line. We see a higher density of ﬂares closer to the minimum. 1 2 3 4 5 (a) (b) 6 ●This histogram hints that there are more ﬂares around the minima of


Planetary Nebulae in the Solar Neighbourhood: Statistics and Luminosity Function David J. Frew(1) and Quentin A. Parker(1,2) 1. Department of Physics, Macquarie University. 2. Anglo-Australian Observatory. Abstract: Recent Hα+[NII] surveys such as the AAO/ UKST Survey and SHASSA have been used to discover a number of new, large, nearby planetary nebulae (PNe). As a result, we have been able to compile a relatively complete census of PNe in the solar neighbourhood, based in part on a new Hα surface brightness – radius relation. We observe an inflection point (or dip) in the cumulative PNLF, seen in both [OIII] and Hβ. Our estimate of the PN formation rate is in quantitative agreement with the white dwarf birthrate. The number of Galactic PNe is estimated to be at least 30,000 and possibly as high as 50,000. Our new PN sample has increased the number of old objects and has allowed the faint end of the PN luminosity function (PNLF) to be seen clearly (see Figure 4). Especially noticeable are the inflection points (or ‘dips’) in the PNLF at M5007 ≈+0.3 and MHβ ≈+2.6. We attribute this to the rapid decline in luminosity of the central star as it starts descending the WD cooling track. While not unexpected, our PNLF’s show this feature clearly for the first time. We also aim to improve the statistics of the total space density and birthrate of PNe, as these parameters are critically dependent on the adopted statistical distance scale for old PNe (Ishida & Weinberger 1987; Pottasch 1996). Allowing for some incompleteness, a preliminary estimate of the PN birthrate is 1.2 ± 0.5 x 10-12 pc-3yr-1, which agrees with the white dwarf birthrate of 1.0 ± 0.25 x 10-12 pc-3yr-1 determined by Liebert et al. (2005). The total population of PNe in the Galaxy is estimated to be >30,000. Planetary nebulae (PNe) are some of the most beautiful and photogenic non-stellar objects in the heavens, and are a “key tool” to understanding the evolution of low- and intermediate-mass stars. The main goals of this study are to provide the most accurate census of nearby PNe in the solar neighbourhood yet compiled, and to refine the statistical distance scale for old PNe. A key adjunct to this project is the AAO/UKST Hα Survey (Parker et al. 2005a). This is a high-resolution, narrow-band Hα+[NII] survey of the Southern Galactic Plane and the Magellanic Clouds using a large, high- quality interference filter on the 1.2-m UKST. The survey comprises narrowband Hα and broadband short-red (SR) photographic images which have been digitized by the SuperCOSMOS measuring machine at ROE. The survey has led directly to the discovery of nearly 1000 new Galactic PNe, mostly of very low surface brightness (see figures 2 & 3). The Macquarie/ AAO/ Strasbourg/ Hα Catalogue (MASH) is a major product of this survey (Parker et al. 2003, 2005b), and includes a good many examples of very large angular diameter, up to nearly 30 arcminutes. Slit spectra have been obtained on a variety of telescopes for all MASH PNe candidates, and we have obtained additional narrow-band imaging to get additional morphological data. We have produced an accurate database of fluxes in the main emission lines for these nearby, highly- evolved PNe. Data has come from our spectroscopy and imaging plus Hα fluxes from the SHASSA and VTSS sky surveys (Frew & Parker in prep.) from which other line fluxes can be bootstrapped via our spectroscopic information. We have also obtained Hα, Hβ, [OIII], and [NII] integrated fluxes for a number of northern PNe using the WHAM Fabry-Perot interferometer (Madsen & Frew, in prep.) D.J.F. has also examined SHASSA images (Gaustad et al. 2001) and has found several new large candidate PNe. Additional WHAM data is being used to determine the nature of several unusual objects (Sh 2-174, DeHt 5, RE 1738+665, Ton 320, PHL 932, Hewett 1, etc), showing that some of these objects are examples of ionized ISM, and are not true PNe. As a result, we have compiled a ‘clean’, statistically-s


U.S. Environmental Protection Agency Office of Research and Development EPA www.epa.gov Building a compendium of expert driven read-across cases to facilitate an analysis of the contribution that different similarity contexts play in read-across G Patlewicz1*, Amanda Ross2, HC Bledsoe2, Janielle Vidal2, Louis Groff1, Brett Hagan1,3, Imran Shah1 1 CCTE, U.S. EPA, RTP, NC, USA, 2ICF Corporation, Reston, VA, USA,3ORAU, Oak Ridge, TN, USA BACKGROUND STRUCTURAL SIMILARITY EVALUATION Grace Patlewicz l Patlewicz.grace@epa.gov l ORCID: 0000-0003-3863-9689 l 919-541-1540 CONCLUSIONS Disclaimer: The views expressed are those of the authors and do not necessarily reflect the views or policies of the US EPA. • 82 Read-across examples cases were compiled from the three main sources. • There were 22 unique decision contexts when aggregated by NAMs, technical guidance or regulatory purposes. • Of the 82 examples, 68 captured regulatory purposes, the remainder were relatively evenly split between efforts to improve guidance or evaluate the utility of NAMs to substantiate read-across justifications. METABOLIC SIMILARITY EVALUATION CLASSYFIRE METHODS The 82 read-across example cases were characterised by 497 individual substances of which 468 could be mapped to a discrete organic structure by way of SMILES To gain a perspective of the chemical diversity across these 468 substances, the chemistry ontology ClassyFire was used to assign structures into their respective chemical class. Some ~63% of the substances were members of either the “Benzene and substituted derivatives” class (25%), the “Fatty Acyls” class (14%), the “Organooxygen compounds” class (12%) or the “Carboxylic acids and derivatives” class (12%). A t-SNE plot based on Morgan chemical fingerprints shows the chemical landscape and is colour coded using a subset of the most populist chemical subclasses. Across the 82 examples, there were 77 different evidence streams characterising the basis for identifying and evaluating the source analogues in each case. The first component of the evidence stream characterising the primary means of identifying candidate source analogues was a structural one in 72 cases, and metabolism in 4 cases. There were 13 different approaches by which analogues were identified. The barplot highlights the main tools and approaches. The OECD Toolbox, DSSTox (within the EPA CompTox Chemicals Dashboard), the NIH’s structure searching tool within ChemIDPlus or some combination of these tools were most common in terms of identifying structural analogues. However by far the most common means of identifying analogues was to look for common scaffolds based on functional groups. • The pairwise Jaccard similarities were computed within each read-across example to explore how structurally similar the target and source analogues were amongst themselves. • The median of the distribution of median values for each case study was determined to be 0.34. (see Fig a) • There was a large degree of variation in pairwise similarities within each case study (See Fig b) Read-across is a data-gap filling technique utilised to predict the toxicity of a target chemical using data from similar analogues. Read-across is predominantly performed as part of an expert-driven assessment which can impeded broad acceptance. Data-driven approaches such as Generalised Read-Across (GenRA) offer scope to generate reproducible read-across predictions where uncertainties and performance are quantified. A key issue is how to reconcile an expert-driven approach with a data driven approach both in terms of how analogues are identified and evaluated as well as how the read-across prediction is derived. An important component of analogue identification and selection is in understanding the contribution that different similarity contexts play, i.e. does structural similarity play a larger role in analogue selection compared with metabolism similarity. This study aimed to explore some of these conside


Unveiling the ICM properties of clusters missed by baryon-selected samples ● Past samples were selected by an observable with covariance with the quantity of interest. These are biased. ● Past works shows that samples non-ICM selected have properties rare (or unique) in ICM-selected samples: – (low) pressure profiles: JKCS041 (z=1.803, SA+2023), IDCSJ1426.5+3508 (z=1.75, SA+21), CL2015 (z=0.05, SA+21). – (variety) Scatter in LX-M, T-M, LX-YX for σv-selected clusters (SA+17, SA+17a,b, SA+19) – (variety) Scatter in LX-M for richness-selected clusters (SA+11) – (variety) Distribution of central X-ray brightness (LX- vs SZ- vs σv-selected, SA+24) ● Present: the baryon selection can be entirely removed by using weak-lensing selected clusters. Performed high- quality X-ray and SZ follow-up of a very few such clusters. These clusters have properties rare (weak and variegate for their mass) in ICM-selected samples (paper submitted). ● AtLAST allows us to unveil the properties of Euclid weak-lensing selected clusters, many of which are weak/faint for their mass (this is one of the AtLAST science cases). Active actions in Euclid consortium (to provide reliable targets). Pilot observations already started with NIKA2, others planned during the MISTRAL commissioning. Stefano Andreon – INAF-OA Brera, Milan, Italy


Exp 359 – Maldives Monsoon and sea level Luca Lanci, DISPEA, Università di Urbino • Dating the sediment (magnetic stratigraphy) • Magnetic stratigraphy Site U1467 drilled in the Maldives platform (Indian Ocean) during Exp. 359, gives a precise record of geomagnetic reversals of the early Pliocene from approximately 5.3 Ma to 3.1 Ma providing a detailed age model in an interval where the biostratigraphic record is scarce. • Searching for bottom currents (anisotropy of magnetic susceptibility) • We use the anisotropy of isothermal remanent magnetization to investigate the statistical orientation of fine magnetic particles and provide data on the strength and direction of bottom currents during the early Pliocene. The strength of bottom currents recorded by the AIRM, shows a prominent increase at the top of Chron C3n.1n (about 4.2 Ma), and the current direction (NE - SW) is consistent with that of modern instrumental measurements. • Why does it matters? • The South Asian Monson is one of the most intense annually recurring climatic elements and of immense importance in supplying moisture to the Indian subcontinent thus affecting human population and vegetation. The seasonal precipitation change is one of the elements most noticed on land, whereas the reversal of the wind regime is the dominating driver of circulation in the central and northern Indian Ocean. Wind-induced currents are an important factor controlling the sedimentation in the Maldives archipelago where they regulate sediment transport from the atoll to the deeper part of the platform as well as the geometry of the sedimentary bodies. The onset of these current-driven drift deposits has been related to the increase of monsoon activity starting in the middle Miocene. ,


The role of gas and dark matter in the dissociative collision of galaxy cluster Abell 2034 Micheli Trindade Moura, Rubens E. G. Machado Universidade Tecnol´ogica Federal do Paran´a (UTFPR), Curitiba, Brazil  micheli trindade.moura@hotmail.com Galaxy Cluster Formation (GCF) 2021 Abstract: Collisions between galaxy clusters are a frequent sce- nario in the hierarchical model of structures. Dissociative collisions provide an extreme environment of interaction between clusters of galaxies, where the properties of dark matter (DM) in relation to baryonic matter become evident. Investigating dissociative scenar- ios allows a deeper understanding of the behavior and dynamics of baryonic and non-baryonic matter in this context of collision. Abell 2034 (z = 0.114) is a bimodal system composed of a north and a south substructure, it has dissociative features observed in X- rays and gravitational lensing. Using N-body hydrodynamic sim- ulations, we present a theoretical study based on the dissociative collision of A2034, aiming to explore the eﬀect that diﬀerent rela- tive concentrations between the clusters generate on the dynamics of the system. We investigated the relationship of the central density ratios with diﬀerent levels of dissociation, where we analyzed nine models with diﬀerent concentrations of the two components: intra- cluster gas and DM halo for each substructure. We found diﬀerent degrees of dissociation that were quantiﬁed by the relative distance between the X-ray emission peak and the dark matter peaks. We found that the ratio of the gas central densities is more decisive than the ratio of dark matter central densities, in determining the level of dissociation for the parameters of this collision. 1 Introduction Dissociative systems are a peculiarity of collisions between galaxy clusters, and are known in the literature since 2006 with the Bullet Cluster (Clowe et al., 2006). Abell 2034 (A2034) is a cluster at lo- cal Universe (z = 0.114) that has dissociative features. The A2034 is composed of two substructures: A2034N and A2034S. The main points about recent developments of the A2034 in the literature are: • The distance between the X-ray emission peak and the south dark matter peak is around 348 kpc, where the distance between both total mass peaks is ∼720 kpc (Monteiro-Oliveira et al., 2018). Implying in a clearly dissociative system, with gas approximately in the middle of two DM peaks. • This merger system has been observed by X-ray satellites since ROSAT (David et al., 1999), and more recently by XMM- Newton (Okabe & Umetsu, 2008) and Chandra (Kempner et al., 2003; Owers et al., 2014). • More recently mass reconstruction are performed by weak gravita- tional lensing method in 2018 by Monteiro-Oliveira et al. (2018). Numerical simulations were developed motivated by the analysis of X-rays and gravitational lensing, leading to a proposed scenario for this collision, where we observed the system at 0.26 Gyr after the central passage (Moura et al., 2021). From this numerical scenario, we carry out a theoretical exploration of the eﬀect that diﬀerent gas and DM concentrations cause in the dissociation of the A2034 merger system. Figure 1. Gas scale length variations, for north (200, 300 and 400 kpc) and south (300, 400 and 500 kpc) substructures. The ﬁrst row shows gas density maps with contours representing total mass. The second row shows projected temperature maps for each model. The third row shows X-rays mocks from counts/pixel and contour curves of the total mass. 2 Simulation setup We aim to investigate the dissociative collision of Abell 2034 using N-body hydrodynamic simulations. To simulate this merger con- text, we set up two spherical galaxy clusters. The code employed and details about the simulation are: • We adopt the Gadget-2 code (Springel, 2005), which uses smoothed particle hydrodynamic (SPH); • The initial conditions are composed of 106 gas particles and 106 DM particles for each cluster; • The


Standardizing Documentation of FITS Headers Although the FITS file format[1] can be self-documenting, human intervention is often needed to read the headers to write the necessary transformations to make a given instrument team’s data compatible with our preferred analysis package. External documentation may be needed to determine what the values are of coded values or unfamiliar acronyms. Different communities have interpreted keywords slightly differently. This has resulted in ambiguous fields such as DATE-OBS, which could be either the start or mid-point of an observation.[2] Conventions for placing units and additional information within the comments of a FITS card exist, but they require re-writing the FITS file. This operation can be quite costly for large archives, and should not be taken lightly when dealing with issues of digital preservation. We present what we believe is needed for a machine-actionable external file describing a given collection of FITS files. We seek comments from data producers, archives, and those writing software to help develop a single, useful, implementable standard. http://virtualsolar.org/ 218.48 J.A. Hourclé NASA-GSFC (Wyle) joseph.a.hourcle@nasa.gov Goal: Create a documentation standard that : • Does not require modifying existing FITS files. • Is machine-readable. • Can be used by software developers to integrate new missions & instruments with minimal effort. The documentation should: • Identify which keywords are using a registered FITS standard and which are discipline, mission, instrument, or processing specific. • Provide units if not explicitly given • Identify range of possible values • Provide expansion / explanation of enumerations • Provide free text to describe how to interpret coded values (eg, SDO ‘QUALITY’), or for additional details. • Identify the authoritative value from a group of similar values. (thus identifying which values are derived, and have more error) References: [1] Pence, et.al. 2010, “Definition of the Flexible Image Transport System (FITS), version 3.0”, http://dx.doi.org/10.1051/0004-6361/201015362 [2] Rots, et.al, (in preparation), “Representations of Time Coordinates in FITS Time and Relative Dimension in Space (V1.0) “, http://hea-www.cfa.harvard.edu/~arots/TimeWCS/ [3] Derriere, et.al, “Vocabularies in the Virtual Observatory, Version 1.19”, http://www.ivoa.net/documents/latest/Vocabularies.html [4] W3C, “Simple Knowledge Organization System“, http://www.w3.org/2004/02/skos/ [5] NASA, “Fits Keyword Dictionaries”, http://fits.gsfc.nasa.gov/fits_dictionary.html [6] NASA HEASARC, (untitled) http://heasarc.gsfc.nasa.gov/docs/fcg/standard_dict.html [7] NOAO, “NOAO FITS Keyword Dictionary: Version 1.0”  http://iraf.noao.edu/iraf/web/projects/ccdmosaic/imagedef/fitsdic.html [8] Shimojo, “The List of SOLAR-B”Hinode” Mission-Wide FITS Keywords”, http://hinode.nao.ac.jp/sbsc/SB_MW_Key13.pdf [9] W3C, “SKOS Simple Knowledge Organization System Primer”, http://www.w3.org/TR/skos-primer/ FITS ‘Keyword Dictionaries’ Although there are many ways of documenting FITS keywords, a number of missions use a ‘keyword dictionary’.[5] The structure varies, as there is no single standard for these documents. For example: [6,7,8] KEYWORD: BITPIX REFERENCE: FITS Standard STATUS: manditory HDU: any VALUE: integer RANGE: -64,-32,8,16,32 COMMENT: bits per data value DEFINITION: The value field shall contain an integer. The absolute value is used in computing the sizes of data structures. It shall specify the number of bits that represent a data value. NAME: Image.naxis[i] KEYWORD: NAXIS%d DEFAULT: none INDEX: 1-9 HDU: primary & extension VALUE: %d UNITS: COMMENT: Number of pixels along axis EXAMPLE: 2048 DESCRIPTION: Required FITS format keywords. Header Keyword: NOAA_NUM Format : Integer Unit/Option : - Category : SOLAR-B Data Source : Chief observer or Proposer of the observation Sample : 11345 Description : The NOAA number of the active region. Note : If the target region doe


Motivation und Zielsetzung Methode und Vorgehensweise Status und nächste Schritte Betreuung Porsche: Betreuung Hochschule: 1. Jahr 2. Jahr 3. Jahr AI Methods in Procurement - Simulation workflow concept - • Case study in the automotive industry with real business data (confidential) • Interesting master thesis project in the newly founded Car I am Digital (CARIAD) organization within the Volkswagen Group • Identify and create further potentials for cost savings and workflow improvements • Provide better data for decision-making • Create tangible added value through AI also for your organization 20210606_SDS_DC_Simulation_Poster_How many buyers does it take to build a car_Mannheim_Spreitzenbarth_Jan.pdf.pptx 2/3 Jan Martin Spreitzenbarth, University of Mannheim Value function: Max periods ௐ௘௜௚௛௧ொ ଵିௐ௘௜௚௛௧் (1) Key performance indicators to be measured may include maverick buying, unmet expectations, attitude towards procurement, idle time and excessive workload, which could answer the research question and prepositions. Motivation Outlook Method Looking forward to further ideas to improve the simulation concept. Looking forward to further ideas to improve the simulation concept. Working prototype for a value function of procurement The conceptual model is iteratively improved through feedback 20210606_SDS_DC_Simulation_Poster_How many buyers does it take to build a car_Mannheim_Spreitzenbarth_Jan.pdf.pptx 1/3 Jan Martin Spreitzenbarth, University of Mannheim Research question: How to solve the organizational sizing problem by maximizing procurement value creation? Preposition I: The sizing problem can be solved by optimizing the value function Preposition II: Simulation shows value of flexibility, e.g., by lean and agile principles Theoretical background KPIs to measure the results Model and train the agents Motivation Outlook Method Simulation of a dynamic system with feedback loops Optimize value creation of procurement through simulation as a work system Design a prototype of a simulation-based procurement work flow system. Design a prototype of a simulation-based procurement work flow system. • Building a working prototype, which can be improved with more data and feedback by the buyers • Looking forward to your feedback to improve the concept 3. Jahr Beschaffungsstrategie Prof. Dr. Heiner Stuckenschmidt 20210606_SDS_DC_Simulation_Poster_How many buyers does it take to build a car_Mannheim_Spreitzenbarth_Jan.pdf.pptx 3/3 Jan Martin Spreitzenbarth, University of Mannheim Theoretical and managerial implications • Value: Organizational sizing can be solved by a value function • Organization: Indirect business functions can be strengthened • Results: The value of organizational flexibility can be shown Limitations, future outlook and questions for discussion • Method: It’s generally easy to challenge the modeling results due to necessary abstraction…what do you suggest to focus on in the design of this study? • Novelty: Which relevant works, theories, and methods would you recommend to investigate further? • Concept: Where do you see shortcomings? What would you do differently? What do you like about it and see potential contributions and advantages? Motivation Outlook Method Thanks for your attention. Looking forward to a good discussion with you! Thanks for your attention. Looking forward to a good discussion with you! Now fun part with implications, questions, and discussion! Looking forward to ways to improve the concept Hr. Joachim Scharnagl Beschaffungsstrategie Jan Martin Spreitzenbarth BCX - Beschaffung Smart Mobility


Coastal and Marine Innovations for Sustainability A worldwide review WHAT are we exploring? 1. What innovations, to improve the quality and/or maintenance of the ES and/or optimize the exploitation of resources in a sustainable way, already exist? 2. How, where, why, when, by whom, and under what conditions were they created? 3. Which ES have been exploited? HOW did we get answers? lines of financing for this type of innovative solutions There is an increasing interest and market for innovations focused on sustainability. The examples found indicate that such innovations can be a feasible solution for tackling: • Habitat loss • Plastic waste • Climate Change • Food shortages Final Remarks WHYare we studying this subject? • Sustainable lifestyle means understanding how our choices impact the world around us and finding ways for everyone to live better1. One way could be through the development of innovations better harnessed for sustainability. This is a key matter to support the implementation of the Sustainable Development Goals2. • If we assess the international trends in the development of innovations that exploit Ecosystem Services (ES) we might be able to understand how innovations have been contributing for sustainability. • Unemployment WHICH are our results? Figure 2. Geographic distribution of innovations, focused on sustainability, found. Figure 3. International trends in the development of innovations focused on sustainability, using coastal and marine ecosystem services as guideline. Figure 1. Tools used in the review. Contacts Cátia Marques (PhD student) catia.a.marques@uc.pt Zara Teixeira (supervisor) zara.teixeira@uc.pt Funding Host Institutions References: 1 Programa das Nações Unidas para o Meio Ambiente (UNEP). (07 de 09 de 2020). Obtido de https://www.unenvironment.org/about-un-environment 2 Walz, R., Pfaff, M., Marscheider-Weidemann, F., & Glöser-Chahoud, S. (2017). Innovations for reaching the green sustainable development goals –where will they come from? International Economics and Economic Policy, 449–480 Marques C, Teixeira Z (2021). Coastal and Marine Innovations for Sustainability. A Worldwide Review. Encontro de Ciência 2021, 28- 30 June 2021, Centro de Congressos de Lisboa, Portugal. DOI: 10.5281/zenodo.14191982


Virtual Folder—Components and Tools Tomáš Kulhánek, Chris Morris, Martyn Winn Scientific Computing Department, STFC Daresbury Laboratory United Kingdom Instruct Biennial 24-26 May 2017, Brno, Czech Republic Public and private deployment Scientific Portal Integration West-Life is a H2020 Virtual Research Environment project that will provide the application level services specific to uses cases in structural biology, covering all experimental techniques (e.g. Xray, cryo-EM, NMR, SAXS), enabling structural biologists to get the benefit of the generic services developed by EUDAT (data storage) and the EGI (grid and cloud- computing). Virtual Folder is one part of this project and aims to perform tasks and workflows of integrative structural biology with focus on data management, stored within different storage providers. The Virtual Folder leverages on software suites of CCP4, SCIPION and others. Supported storages: B2DROP, provided by EUDAT; commercial DROPBOX; any data storage providing standard WEBDAV interface (here PCLOUD). File Manager lists all files within connect- ed storages. It can show a RAW file con- tent in Text View mode —or render a view in LiteMol viewer able to visualize protein structure defined in PDB format or BinaryCIF. (Up) File Picker and Upload Dir Picker components re- ferred from third party portal. They return unique URL link- ing to file or directory.(Right) Pilot integration into DISVIS service opens popup window with File Picker http://milou.science.uu.nl/cgi/services/DISVIS/disvis/ Custom Dataset System Architecture Guide Public (Up) https://portal.west-life.eu Default portal web site. (Right Up) Login redirects to Instruct ARIA Login/ Register. (Right) After Login, User specific view of his Virtual Folder is presented. Private (Bottom) Single user. Download VM image from ap- pdb.egi.eu (OpenStack and OpenNebula templates based on Cern-VM) (18MB), bootstrap downloads additional ~100MB. Source codes https://github.com/h2020-westlife-eu/west-life-wp6 Vagrant (VM + environment) git clone https://github.com/h2020-westlife-eu/wp6-vm.git cd wp6-vm vagrant up Scientific Software Integration Scipion tools (Up) CCP4 suite (Down) access di- rectly files in the West-Life Virtual Folder. (private deployment only) Development Environment (Left) Dataset definition compo- nent. Integrated with autocomplete and other services and visualization components of Protein Databank in Europe by EMBL EBI[1], PDB-REDO databank by NKI[2] and Protein resi- due interaction by MRC Laboratory of Molecular Biology[3] [1] https://www.ebi.ac.uk/pdbe/pdb-component-library/ [2] http://pdb-redo.eu/ [3] http://www.mrc-lmb.cam.ac.uk/rajini/about.html Project partners: European Molecural Biology Laboratory (EMBL), Utrecht University (UU), Science and Technology Facilities Council (STFC), Netherlands Cancer Institute (NKI), National Institute of Nuclear Physics (INFN), Masaryk University (MU), Spanish National Re- search Council (CNIC), Consorzio Interuniversitario di Risonanze Magnetiche di Metalloprotaine (CIRMMP), Instruct, Luna Technologies. This project is funded by Horizon 2020 West-Life is part of the e-Infrastructure Virtual Research Environment (VRE) project No. 675858


Innovative Research for a Sustainable Future www.epa.gov/research Danica DeGroot l degroot.danica@epa.gov l 919-541-2482 Alginate Immobilization of Metabolic Enzymes (AIME) for High-Throughput Screening Assays Danica E. DeGroot, Russell S. Thomas, Steven O. Simmons National Center for Computational Toxicology, U.S. EPA, Research Triangle Park, NC Objective Materials & Methods Results & Conclusions Future Directions To develop a platform to retrofit existing high-throughput screening assays with metabolic competence. Introduction The EPA’s ToxCast program utilizes a wide variety of high-throughput screening assays (HTS) to assess chemical perturbations of molecular and cellular endpoints. A key limitation of many HTS assays used for toxicity assessment is the lack of xenobiotic metabolism which precludes the detoxification as well as toxic bioactivation of chemicals tested in vitro, thereby mischaracterizing the potential hazard posed by these chemicals. To address this deficiency, we have developed the AIME platform to retrofit existing HTS assays with extracellular xenobiotic metabolism. By encapsulating human hepatic S9 in alginate microspheres, cytotoxicity and assay interference associated with direct addition of S9 is reduced. Here we demonstrate the deployment of the AIME platform to the MSTI Fluorescence-Based Thiol-Reactive Assay™. Chemicals – All chemicals were purchased from Sigma-Aldrich and stock solutions were prepared in DMSO. Alginate Immobilization of Metabolic Enzymes (AIME) – Human hepatic S9 (BioreclamationIVT; Lot OUL or TOF) was encapsulated in alginate microspheres using a modification of a cell encapsulation protocol by Lee et al. (1). In Figure 1, AIME microspheres are shown attached to a prototype lid for 384-well plates. In Figures 2-4, microspheres were made using either 10% or 20% S9 and attached to commercially available Nunc polystyrene replicators (for 96-well plates). Cytochrome P450 (CYP) Enzyme Activity – CYP enzyme activity was quantified using Promega P450-Glo kits with the following modifications: assays were performed in DMEM/1% FBS and substrate incubations were standardized to 1 hour. All assays included an NADPH regeneration system per the manufacturer's protocol. AIME Coupled MSTI Fluorescence-Based Thiol-Reactive Assay – The MSTI Fluorescence-Based Thiol-Reactive Assay™(2) was used to identify electrophilic compounds with and without metabolic competence. Eighty test compounds (prepared at their solubility limit in DMSO) were acoustically dispensed into triplicate black, 96-well plates followed by the addition of phosphate buffer (pH 7.4) containing an NADPH regeneration system (DMSO 0.1% (v/v) final concentration). AIME lids containing active human hepatic S9, heat-inactivated S9 or an empty microsphere (no S9) were added to the plates and allowed to incubate with the test compounds for 1 hour at 37ᵒC. The lids were removed and a 2X MSTI (30 µM final concentration) was added. Acetyl-MSTI was used as a positive control. The plates were incubated at room temperature for 30 minutes followed by quantitation of the fluorescent signal (excitation: 510 nm; emission 650 nm). An increase in electrophilicity was detected as a decrease in the fluorescent signal. CYP1A2 CYP2B6 CYP2C9 CYP3A4 0 10 20 30 Fold Activity/Free S9 (1 µg) Hours Percent Activity 0 1 2 3 4 5 0 50 100 150 CYP1A2 CYP2B6 CYP2C9 CYP3A4 Figure 2: Encapsulated human hepatic S9 maintains measurable cytochrome P450 activity over time AIME microspheres were incubated at 37oC in DMEM/1% FBS for the indicated time. CYP-specific pro-luciferin substrates were then added and the reaction was allowed to proceed for 1 hour prior to detection of the luciferin metabolite. Data are normalized to activity at t=0. Inset: relative CYP activity at t=0. Values represent the mean ± SEM of three measurements. -3 -2 -1 0 1 2 3 0 50 100 150 CYP1A2 [Compound] log µM Percent Activity -3 -2 -1 0 1 2 3 0 50 100 150 CYP2B6 [Compound] log µM Percent Activi


Results and discussion 11 Tailoring birefringence of LIPSS through thin film coatings A. San Blas1,2, N. Casquero1,2, S. Sánchez1,2, L.M. Sánchez-Brea3, J. Buencuerpo3, A. Rodríguez1,2, S.M. Olaizola1,2 1 Ceit, Manuel Lardizabal 15, 20018 Donostia / San Sebastián, Spain, 2 Universidad de Navarra, Tecnun, Manuel Lardizabal 13, 20018 Donostia / San Sebastián, Spain 3 Applied Optics Complutense Group, Optics Department, Universidad Complutense de Madrid, Facultad de Ciencias Físicas, Ciudad Universitaria s.n., 28040, Madrid, Spain asanblas@ceit.es Introduction Experimental Conclusions Laser-induced periodic surfaces structures (LIPSS) present some degree of birefringence due to the orientation of the nanoripples. Among other properties, this makes LIPSS a good candidate for applications involving polarization changes such as reflective polarization gratings, polarimeters, displays or polarizing beam splitters. SHORTCOMING - Low parameter tunability. Fabrication of LIPSS in a small depth and periodicity range. SOLUTION - Introduction of a new degree of freedom. Thin film dielectric coatings modify the optical properties of the material, specifically, reflectivity and birefringence. Dielectric thin film coatings are a good candidate for the tunability of LIPSS birefringence. Reflectivity can be improved for certain wavelengths by varying coating thickness. LIPSS irregularities need to be fixed or taken into account. Coating structure is conformal rather than uniform. Preliminary study with promising results, further research is being carried out. Acknowledgements Characterization Determination of the reflectivity in a broad spectrum (400-1100 nm) with a supercontinuum laser and an integrating sphere to collect the reflected light with output for a spectrometer. Transversal profile and film thickness analysis by means of SEM (Scanning Electron Microscopy) and FIB (Focused Ion Beam). Platinum is deposited on the sample before the FIB procedure in order to protect the surface of the sample. Fabrication Stainless steel samples processed with femtosecond laser focused on the surface of the sample to fabricate nanostructures (LIPSS) with an estimated period of 600 nm and depth of 300 nm. The sample was moved with X, Y and Z stages at a speed of 1 mm/s, processing lines of 10 μm width in each pass, moving in parallel trajectories until the desired size is achieved. Pulse energy used was 1 μJ. Polarization of the laser was controlled with a half-wave plate so that the orientation of the generated LIPSS were perpendicular to the direction of the processed lines. The samples were cleaned in a 5 minutes ultrasonic bath with acetone. Polarization changes induced by the sample were measured with a polarimeter using a half-wave plate in combination with 633 and 808 nm continuous laser sources at 25º incidence with respect to the surface normal and linear polarization at 45º with respect to LIPSS orientation. Afterwards, thin film coatings of thicknesses 50, 100, 200 and 300 nm of SiO2 or Si3N4 were deposited in the different samples through PECVD (plasma enhanced chemical vapor deposition). Numerical simulations FDTD simulations of the effects on polarization as a function of LIPSS parameters (depth, periodicity) and coating thickness in SiO2. Coating structure and LIPSS characterization In the range of thicknesses used in this experiment, the coatings present good conformality to LIPSS. Promising result regarding the numerical simulations, which show that the structure of the coating is a key parameter. The profile of LIPSS shows irregularities with average depth of 340 nm. LIPSS periodicity of ~630 nm has been measured through 2D-FFT analysis of the SEM images. Reflectivity High reflectivity is very desirable for potential applications. Our results show that thin film coatings modify LIPSS reflectivity in certain regions of the spectrum depending on coating thickness and material, improving LIPSS performance. Birefrin


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Improving the workflow of real-time fMRI Neurofeedback experiments Michael Lührs1,2, Florian Krause1,2, Caroline Benjamins1,2,Sascha Brunheim1,2, Judith Eck1,2, Quentin Noirhomme1,2, Mona Rosenke1,2, Rainer Goebel1,2 1Department of Cognitive Neuroscience, Maastricht University, Maastricht, Netherlands 2Brain Innovation B.V., Maastricht, Netherlands, Introduction Methods During the last decade the number and complexity of real-time functional magnetic resonance imaging (rtfMRI) neurofeedback (NF) experiments has dramatically increased. We developed a new software to improve the workflow of standard rtfMRI NF experiments and thus make these applications more accessible for clinical settings. The newly developed software combined with the improved workflow promises to reduce the amount of possible errors when running rtMRI NF experiments as well as the required scanning time. These developments can be applied to multiple MR systems and experimental designs allowing even novel experimenters to run complex rtfMRI NF experiments. We applied the workflow on a Siemens Prisma scanner in combination with Turbo-BrainVoyager 3.2, a real-time analysis software for fMRI data and Expyriment [1] for stimulus as well as feedback presentation. Results The workflow was used in nine rtfMRI experiments with three different subjects. Each experiment consisted of 54 minutes of scanning. We were able to complete five runs in each session in less than 90 minutes including the acquisition of pulse and respiration data. The Scan Session Tool hereby aids in recording all scanning details in a standardized way and allows the automatic organization of all scanning related data and files, thus facilitating/simplifying multi-center studies. The FBE shows a delay in data transfer times compared to the COE. The jittering in FBE s not present in COE. The COE was in mean 293.37ms faster than FBE. Standard deviation in FBE (87.14ms) was much higher compared to COE (18.29ms) due to the observed jitter and file read write procedures. The jittering artefact seems to be related to data size and TR. Similar results were observed using a Siemens 3T Prisma MRI. Conclusion We showed that standardizing the procedure of rtfMRI NF experiments is important to decrease the probability of errors while at the same time reducing the difficulty to perform an rtfMRI NF experiment. Additionally we showed that the connection oriented data transfer procedures of fMRI images leads to an decrease in transfer time variability. The research leading to these results has received funding from the European Community's Seventh Framework Programme FP7/2007-2013 under grant agreement number PITN-GA-2011-290011. Usual rtfMRI NF experiments consist of multiple scan sessions for which the main structure is the same or similar for all sessions and subjects. Assuming this repetitive structure, the improved workflow offers several small automatized procedures to simplify the tasks involved in running the experiments. As a first step we predefine one rtfMRI NF experiment session and prepare all necessary settings and components in advance. These configurations can be used for the session without changing the settings during the experiment. One example workflow (Figure 1) consists of a structural scan followed by a functional localizer (FL) and multiple NF runs. Figure 1: rtfMRI NF experiment workflow 900 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 2100 2200 2300 2400 2500 2600 2700 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 Time after trigger (ms) Volume Data transfer times (Siemens 7T Magnetom) 1000TR Fast 1000TR Slow 1250TR Fast 1250TR Slow 1500TR Fast 1500TR Slow 1750TR Fast 1750TR Slow 2000TR Fast 2000TR Slow Because of errors and problems t


Gaps and rings at ice lines: Icy pebbles and small rocky dust Katrin Ros, Lund University katrin.ros@astro.lu.se We modelled particle growth by condensation around the water ice line: The result is a bimodal size distribution with fast-growing icy pebbles locally at the ice line and small, rocky dust particles spreading out over the disc. This supports the idea of disc substructures forming at ice lines. Distinguishing between nucleation of ice on rock, and deposition of vapour on already ice particles, is the key to growth. 2000 4000 6000 8000 10000 t/yr 0.01 0.10 1.00 amax/cm 2B, nucl+dep 1B, dep only Key concept: Vapour can freeze out on icy particles, but not on bare rocky surfaces. Why? 0 1 2 3 4 5 Scrit 2.0 2.5 3.0 3.5 4.0 4.5 r/au 180 160 140 T/K Scrit, sil Scrit, ice rice sublimation deposition nucleation S ice line Radial distance S Particle size 1.0 0.1 Icy particles (blue) grow when vapour (red) freeze out on them — vapour deposition. Result: The difference between nucleation and deposition is the key to growth Result: Icy fast-growing pebbles at the ice line, small rocky dust elsewhere Sketch of the model ice line Critical saturation ratio for deposition on ice Scrit,ice=1 (blue), and for nucleation on silicate Scrit,sil=-0.0626T+13 (black), experimentally determined by Iraci et al (2010). The saturation ratio we find in our model is overlaid as a grey dotted line. This saturation ratio allows for sublimation and deposition, but never reaches high enough values for nucleation. Starting from micron-sized and millimeter-sized particles, after 1000 years we find cm-sized icy pebbles at the ice line, while surrounding regions are dominated by small dust. Blue is ice-covered particles, black is bare, rocky particles. Collisions are not included in this model. 2.0 2.5 3.0 3.5 4.0 r/au 0.0001 0.0010 0.0100 0.1000 1.0000 a/cm (b) Locally at the ice line: deposition-dominated growth to icy pebbles Sublimation region: small rocky dust. No growth: small particles diffuse out over the disc ice line simple model full model Pebbles move across the ice line due to radial drift (grey left- pointing arrows). Sublimation results in a silicate nucleus (black) and water vapour (red). Turbulent diffusion (grey swirls with right-pointing arrows) spread vapour and small particles out over the disc. Bare dust grains cannot get a new ice mantle (nucleation) since the vapour pressure at the ice line is too low. All vapour is instead deposited on icy particles as in 1. Saturation ratio: vapour pressure/ saturated vapour pressure 1 2 3 4 5 In our full model (blue) where we included the difference between nucleation and deposition icy particles grew from mm to cm. In a simplified model (red) where vapour freezes out equally well on rock and ice most of the vapour condenses on small dust, and particles did not grow above mm. Ros, Johansen, Riipinen & Schlesinger, A&A, 2019


P THE COMMUNICATORS MANAGE CITIZEN SCIENCE PROJECTS, DESIGN AND IMPLEMENT PUBLIC ENGAGEMENT STRATEGIES, DEVELOP INNOVATIVE AND OPEN EDUCATION MATERIALS AND ACTIVITIES, DISSEMINATE SCIENCE IN SOCIAL MEDIA, SUPPORT SCIENTISTS IN DEVELOPING THEIR OWN OPEN COMMUNICATION PRACTICES, ENDORSE OPEN SCIENCE, COMMUNICATE ACROSS DIVERSE DISCIPLINES AND SECTORS… THE EVALUATORS DESIGN A HOLISTIC RESEARCH EVALUATION FRAMEWORK, INNOVATE INDICATORS, DRIVE A CULTURAL CHANGE, ALIGN EVALUTION METRICS WITH RESEARCH STRATEGIES AND MISSIONS, DELIVER EXPERT RECOMMENDATIONS TO FUNDERS AND POLICY MAKERS… TRAINING THE ARCHITECTS DESIGN AND MANAGE DIGITAL INFRASTRUCTURE, TECHNICAL SUPPORT FOR USERS, ENSURE INSTITUTIONAL COMPLIANCE WITH THE LEGAL STANDARDS AND FRAMEWORKS FOR: BUILDING INFRASTRUCTURE, ACCESSING, MANAGING AND SHARING RESEARCH OUTPUT WITHIN AN OPEN SCIENCE SYSTEM… Open Science advocacy influences research in three interdependent areas: research policies and practices, the role of society in research, and the management of research output. To fully embrace and adopt Open Science principles, research institutions need dedicated professionals at the interface of research and society to empower the actors involved. In this poster we present the “Documentalists Working Group” in Catalonia as a use case of a bottom-up community of practice helping their centres to successfully ride the wave of Open Science. The “Documentalists Working Group” brings together professionals from ten Catalan research centres (BSC, CRG, IBEC, ICFO, ICIQ, ICN2, IDIBABS, IFAE, IRB Barcelona, IRTA) and is a community of practice on management of research output. The objective of this group is to define synergies in workflows in scientific publication and research data management, to tackle challenges through peer-to-peer learning, and consult experts jointly. We will also explore how Open Science devises new career paths for researchers and research related professionals following the demand for Open Science facilitators that (i) engage and train researchers to participate in Open Science practices, (ii) provide opportunities to collaborate with the society, (iii) support the development and implementation of new evaluation schemes, and (iv) provide access to needed infrastructure. The Role of Research Management in Open Science DOCUMENTALISTS Working Group in BCN Publication Management & Reporting Open Access to Publications Training OA, Licensing To be a community of practice of professionals with Documentalist’s responsibilities at research centres in and around Barcelona. Increase awareness in research community TASKS CHALLENGES COLLABORATORS 3.100 Publications per anno 2.600 Researcher Associated Univ. libraries CSUC Mary Hirsch and Sonja Reiland Centre for Genomic Regulation, Barcelona Institute of Science and Technology IDEA & PROPOSAL TEST & METHOD DATA & Obs. MODEL CODE RESEARCH Articles EDUCATE & Train Open Educational Resources OPEN RESEARCH LIFECYCLE REVIEW ENGAGE POLICY Context References • FosterOpenScience, Open Research Lifecycle modified version of "Open Science by Default" Workflow doi: 10.5281/zenodo.49960 • Fecher B., Friesike S. (2014) Open Science: One Term, Five Schools of Thought. In: Bartling S., Friesike S. (eds) Opening Science. Springer, Cham • OpenUp Project D 4.2 [2018] Role description: Dissemination to businesses and the public, • Mary Auckland, RLUK, [2012] Re-skilling for Research, An investigation into the role and skills of subject and liaison librarians required to effectively support the evolving information needs of researchers Research Data Management (RDM) Policies from Funders RDM Policy workshop in BCN Collaborative development of a workable Research Data Management Policy for practical research support. FOLLOW-UP Cross sectional expert and multi- institutional working Group meetings Cross sectional expert board meetings at each participating institute MISSION MISSION Organised by Documentalists Working Group and instructed by Pa


Abstract Parkinson’s disease (PD) involves the generation of dopaminergic (DA) neurons in the substantia nigra, causing tremor and motor impairment. Defects in PARK2 protein are the cause of Parkinson’s disease type 2 (PARK2), also known as early-onset parkinsonism with diurnal fluctuation (EPDF) or autosomal recessive juvenile Parkinson’s disease (PDJ). In mouse model, Parkin is critical for removing dysfunctional mitochondria via autophagy. The absence of Parkin does not affect turnover under normal conditions. However, during stress conditions, Parkin helps to remove damaged mitochondria by autophagy. Innoprot has developed a novel fluorescence cell-based assay for High Content Screening to screen compounds that can promote Parkin2 mithocondrial localization without mithocondrial stress induction. In this work, we used this model to screen a library of 1200 compounds. After the screening campaign, the positive compounds were chosen for further testing, based on the strength of the initial response and the lack of cytotoxicity. Our results indicate that Parkin2 cell-based assay is a valid strategy for drug screening. Fluorescence-Based Assay Development To Screen Drugs Against Parkinson´s Disease Salado C., Kortazar D., Mella R., Roura-Ferrer M., Gámiz J. & Villacé P. Innoprot (Derio, Spain) www.innoprot.com Schematic representation of the model OMM IMM OMM IMM CCCP tGFP MTS Parkin Parkin FP602 FP602 tGFP MTS Parkin Parkin FP602 FP602 Figure 1. Schematic representation of Innoprot’s Parkin2 model in living cells. Parkin2 model is a fluorescent fusion polypeptide capable of changing its localization within the cell from the cytosol to the outer mitochondrial membrane, after CCCP addition. Parkin2 forms part of E3 ubiquitin ligase complex that mediates the targeting of proteins for degradation. CCCP is a mitochondrial proton gradient uncoupler that induces a loss of mitochondrial membrane potential (m) and therefore, promotes recruitment of Parkin2 to damaged mitochondria during the early stages of mitophagy. The second fluorescent fusion polypeptide (MTS-tGFP) allows us to visualize stability and location of mitochondria. High-content screening (HCS) in Parkin2 model uses living cells as a tool in biological research to discover and optimize new drug candidates against Parkinson’s disease. Materials and methods Cultured cells: PARK2-FP602+MTS-tGFP cell line has been obtained by a double transfection of an ex- pression vector that encodes turbo green fluorescent protein (tGFP) fused to mitochondrial targeting sequence (MTS) derived from the subunit VIII of human cytochrome C oxidase and a vector containing PARK2 protein fused to FP602 red fluorescent protein. U2OS PARK2-FP602+MTS-tGFP cell lines were cultured into 96 wells Imaging Plates (BD) at 0.25 cells/cm2 in 200 l of DMEM 10% FBS and incubated at 37ºC and 5% CO2. Image adquisition: human tagged PARK2-FP602+MTS-tGFP stably expressing cells were treated with CCCP (positive control) at 5 M for 3 hours and library compounds at 10 M for 24 hours in Optimem. After that, the nucleus was stained with Dapi and the presence of recruited Parkin to defective mito- chondria was quantified by fluorescence using image analysis algorithms and the BD Pathway 855 image platform. Cellular viability and granularity was calculated relative to the negative control. Results Figure 2. Representative data of Parkin2 redistribution screening. An initial screening of 1200 compounds were done but toxic compounds (with a cell viability under 60% with respect to control) were eliminated from the study. Negative control (DMSO) is represented in pink and positive control (CCCP, 5 M) is represented in green. Positive compounds (with an activity over 1.5 with respect to control, I-VIII) are represented in yellow. Parkin2 redistribution was quantified by measuring fluorescence granularity. Figure 3. Colocalization assay of Parkin2 and mitochondria in presence of redistribution-positive compounds (I-V


Genapp GenApp WillItFit QuaFit Others … UI – GUI – Web GUI HPC – Gateway The computational code Build Calculate Simulate Analyze SCT SASSIE Bringing higher end computational tools to the bench scientist to accelerate the discovery process. Current grant coming to a close. Working on transitioning to Community efforts and how to coordinate new efforts going forward. Several new projects being proposed -- plus • Networking/workshop grants • COST action proposal in Europe • looking at various US opportunities • Nurture involvement from major facilities OTHER IDEAS WELCOME


Target muscle activation level was 10±3% of RMS MVC when active and < 10 µV at rest. TMS Delivered at 120% active motor threshold (AMT) for each muscle. Each map used 120-150 pseudorandomly placed stimulations [3]. Data collection and analysis Coil and head 3-D positions and orientations, and MEPs, at times of TMS stimulation were saved using custom soft ware*. 3-D stimulation locations were fitted to a sphere, then represented in 2-D spherical coordinates. The cloud of MEP values & positions was resam pled onto a regular grid (MEPest) using radial ba sis function interpolation. Is muscle co-activation preferentially driven by overlapping cortical map regions? Matthew J Ward, John Cirillo, Angus J C McMorland Department of Exercise Sciences, University of Auckland Overlapping cortical muscle maps: a substrate for synergistic activation? Fast, optically tracked, dual-muscle TMS mapping Contact information Dr Angus McMorland a.mcmorland@auckland.ac.nz * https://github.com/mwar136/TMS-map ping-and-analysis References head marker coil marker on end AD electrode FDI electrode Optical tracking (Polaris, NDI) of coil and head during mapping and recording of EMG from AD and FDI. Tasks Repetitive actions, in time with a metronome (4 - 5.5 s per stim): 1) thumb-index finger pinch; FDI map only 2) push peg with palm, from shoulder; AD map only 3) combined - pinch & push; map both muscles AD (blue) and FDI (red) muscle maps during in dependent (A) and synergy (B) movements. Maps show large overlap, particularly of highly excitable areas. Centre of gravity (COG) of FDI (red square) is lateral of AD COG (blue triangle) in both maps. Position is relative to Cz. FDI AD 40 -20 -80 -10 ML (mm to Cz) AP (mm to Cz) Motor cortical representations of proximal and distal muscles, such as anterior deltoid (AD) and first dorsal interosseous (FDI), overlap [1, 2]. Motor evoked potential (MEP) amplitude in response to transcranial magnetic stimulation (TMS) of motor cortex. 1. H. Devanne et al., The comparable size and overlapping nature of upper limb distal and proximal muscle representations in the human motor cortex. Eur. J. Neurosci. 23, 2467–2476 (2006). 2. J.-A. Rathelot, P. L. Strick, Muscle representation in the macaque motor cortex: an anatomical perspective. Proc. Natl. Acad. Sci. U. S. A. 103, 8257–8262 (2006). 3. M. van de Ruit, M. J. L. Perenboom, M. J. Grey, TMS brain mapping in less than two minutes. Brain Stimul. 8, 231–239 (2015). 4. H. Devanne, L. G. Cohen, N. Kouchtir-Devanne, C. Capaday, Integrated motor cortical control of task-related muscles during pointing in humans. J. Neurophysiol. 87, 3006–3017 (2002). Overlap of cortical muscle maps may facilitate synergistic co-activation of muscles. This study tests the hypothesis: During synergistic tasks, overlapping portions of the muscle maps are preferentially activated compared to non-overlapping regions. Participants Participant Age (Years) Sex Laterality Index (%) AD AMT (%MSO) FDI AMT (%MSO) 1 22 F 100 58 38 2 21 M 100 78 40 3 22 M 67 68 36 4 32 M 100 46 34 5 21 M 100 33 28 6 22 M 100 66 45 7 22 M 100 70 46 8 31 F 100 60 33 9 21 F 67 69 30 10 23 M 100 66 25 11 21 M 71 60 44 12 21 F 100 68 38 13 23 M 100 63 46 Mean 23.2 69% M 92.7 61.9 37.2 SD 3.75 14.0 3.2 1.9 1. No evidence for preferential excitation of over lapping regions of muscle maps for synergistic activation. 2. No change in overall excitability in the syner gistic task suggests that FDI and AD are inde pendent in terms of co-activation, which has im plications for horizontal connectivity (excitatory or disinhibitory) between the networks associated with these muscles. 3. FDI may be ‘special’; previous studies suggest coactivation of FDI and ECR is not similarly inde pendent [4]. All subjects were healthy and right-handed. As expected, active motor threshold was lower (above), and MEP amplitude was higher (below) in FDI than AD, because direct corticospinal projections innervate distal muscles more than proximal.


www.gapmaps.org Sharing and modeling groundwater contamination data with the Groundwater Assessment Platform (GAP) Fields of application Joel Podgorski and Michael Berg Eawag, Swiss Federal Institute of Aquatic Science & Technology, Dübendorf, Switzerland (contact: joel.podgorski@eawag.ch) Create contamination hazard prediction maps GAP layers L. Rodríguez-Lado et al., Ground- water arsenic contamination throughout China. Science 341, 866–868 (2013). Modeling is conducted using logistic regression. Users supply their own point data as the dependent variable. Independent variables may be chosen from the publicly available GAP layers or user-supplied raster or polygon layers. The model may be used to create a map and exported as a GeoTIFF. Model output includes statistics on independent variables, model classification and an ROC curve China arsenic prediction map produced through statistical programming Comparison modeling offline vs. GAP Nearly identical map produced with much less effort in GAP Groundwater contamination maps SE-Asia arsenic prediction map Pakistan arsenic prediction map General modeling procedure GAP contains various global, regional and country-level prediction maps of geogenic groundwater contamination. These have been produced by geostatistical modeling of measured concentration points with spatially continuous environmental predictor varia- bles. The maps indicate areas that are likely highly contaminated and require testing. Introduction: The Groundwater Assessment Platform (www.gapmaps.org) is a free, interactive online GIS platform for the mapping, sharing and statistical modeling of groundwater quality data. Users can upload and control the display parameters of their geospatial data as well as share their data with individual users, community groups or the public. The modeling functionality uses logistic regression and allows users to take advantage of publicly available global datasets of various environmental parameters to produce prediction maps of their contaminant of interest. Upload own data and manage display settings Upload rasters, shapefiles or point data Set the symbology, e.g. filters, symbols Swiss Federal Institute of Aquatic Science and Technology Areas of application Although originally dedicated to addressing the geogenic groundwater contaminants arsenic and fluoride, GAP was de- signed in a general manner such that it can be applied to any other geospatial contaminants or measurements of interest. Possible other applications include anthropogenic contaminants (e.g. nitrate, salinity) or soil contaminants. Share data with a user, community or the public Communities Example of publicly shared data


Poster title:Modelling of energy demand for e-pentration technologies in the transport sector :In the context of Ethiopia. Author(s):Tessema,Tegen Dagnew Affiliation(s):Bahir Dar University,Ethiopia 1. Context Contact: names and emails 5. Policy insights, conclusions and future work 3. Methods & Scenarios Scenarios: • The current energy source of the transport sector of country is mainly depends on Petroleum\fuels.However,the price of petroleum highly affect the transport sectors as the country doesn’t have its on production of petroleum energy supply chains . • As a result, penetration of additional technologies like e-vehicle by 50% by the next 2050’s,could be a good startegey as a viable Technology mix to satisfy the demands of the transport sector . Assumptions: The potential of petroleum fuels in the future will be diminishing, and could not satisfy the transport sector energy demand. A switch to e-technologies at a penetration level of 15% increment in every decade to the level of 60 % can improve the energy security of the country. 2. Aim Socio economic profile of Ethiopia: • Ethiopia ,Landlocked country in the horn of Africa • Total area 1.13million square.km. • Population estimated (2020):117.87million • Urban population:24.7527 million,(21%) • Rural population :93.118million(79%) • GDP:$925USD •As of July 03/2023,the transport sector of Ethiopia heavily depends on petroleum oils with an import of 3.72 billion cubic meters per annum of petroleum for 72.60 billion Birr with a sky rocketed price for every year. •A switch to e-technologies at a penetration level of 20% increment in the coming 2050 years level of 60 % can improve the energy security of the country. 4. Results Country Flag 6. References References : References : 1.Sustainable Development Goals Report - United Nations Sustainable Development,acess date,July 11,2023 2.EA – International Energy Agency,acess date,July 10,2023 3.The Ethiopian energy sector and its implications for the SDGs and modeling Amsalu Woldie Yalewa’ Foscari University of Venice, Via Torino 155, Venice 30172, Italy 4.Climate Compatible Growth – Providing research and global public goods to help countries develop strategies, plans, and policies to attract investment into low-carbon growth opportunities.,acess date,July 12,20223 5.Ethiopia Energy Statistics - Worldometer (worldometers.info) The aim of this study is to investigate the impact of switching of e- vehicles in the final energy demand to the context of Ethiopian transport sector . • Here in ,the energy demand modeling were carried out in the transport sector of Ethiopia .we can realize that there is an increase in energy demand by 10% (average)when we switch the e-vehicles by 60% by the next 2050 as compared to the BAU scenario. • Moreover,the energy demand share of by the mobility activity by bus and car takes a huge share of (48%) as compared to freight and international transport. • As a policy insight, the result which was been obtained from this modeling probably have some discrepancy with the realty as there is little well organized data policy on the transport sector of Ethiopia. • To promote the e-vehicle in the Ethiopian transport sector, the charging infrastructure should the Ethiopian government as the first of the first priority to adopt at good penetration level. • Massive deployment of e-vehicles in the public transport and freight activities. • ICTP JOINT SUMMER SCHOOL FOR SUSTAINABLE DEVELOPMENT | 2023 Figure #. Caption. A Cost-benefit analysis of Policy, Programs and Projects (C3PO) that is Retrievable, Reusable, Repeatable, Reconstructible, Interoperable and Auditable (u4RIA) (A) (B) Tessema ,Tegen dagnew,email:tegi1246@gmail.com CC CC DC


Parestesias misteriosas: trastorno orgánico vs síndrome conversivo. María Ríos Vaquero1, José Barana 2, Gloria Lorenzo Chapatte1, Lorenzo Rojas Vázquez1, Teresa Jiménez Aparicio1, Luis Sobrino Conde3. 1: MIR. Servicio de psiquiatría. Hospital Clínico Universitario de Valladolid.; 2: MIR. Servicio de psiquiatría. Centro Asistencial Universitario de Palencia.; 3: PIR. Servicio de psiquiatría. Hospital Clínico Universitario de Valladolid. Se presenta un caso en el que se expone la dificultad del abordaje clínico a la hora de filiar episodios con sintomatología de posible etiología diferente frente a la categorización teórica de los mismos. Se expone el caso de una mujer de 26 años de edad, de origen extranjero que visita en repetidas ocasiones en el servicio de urgencias por presentar episodios sincopales y alteración en la sensibilidad en hemicuerpo y hemicara izquierdos que tras estudio por neurología impresiona de posible síndrome conversivo. Sin embargo al realizar historia completa en las sucesivas visitas se detallaron otros posibles factores de interés como síndrome de churg Strauss del padre, epilepsia y valvulopatía mitral en hermana gemela y sintomatología depresiva en su madre. Se le cita para realización de varias pruebas complementarias a las que la paciente no acude, lo que llama la atención. -RMN cerebral: Cisterna Magna Vs quiste aracnoideo retrocerebeloso. Nódulos subependimarios múltiples. -RMN cervical sin hallazgos reseñables. -RMN dorsolumbar, EEG y holter: no acude. En este caso planteamos realizar el diagnóstico diferencial con diferentes patologías que también cursan con misma sintomatología como el sd. Churg Strauss, compresión vertebral de raíces nerviosas o causa funcional. Se realiza seguimiento en consultas de neurocirugía y de psiquiatría realizando tratamiento con escitalopram y alprazolam con ligera mejoría. Además realiza psicoterapia. En este caso no queda claro la presencia de patología orgánica dada la poca colaboración a la hora de realizar pruebas complementarias. Si bien esto apoya la impresión de ser de causa funcional o simulada. Revisando la literatura científica en algunos casos es dificultoso esclarecer la etiología del cuadro y se debe tener en cuenta una visión multidisciplinar abordando el caso en su conjunto. INTRODUCCIÓN Y OBJETIVOS DESCRIPCIÓN DEL CASO PRUEBAS COMPLEMENTARIAS DIAGNÓSTICO DIFERENCIAL Y TRATAMIENTO DISCUSIÓN Y CONCLUSIONES BIBLIOGRAFÍA: