Innovative Research for a Sustainable Future www.epa.gov/research Enriched pathways uniquely associated with 4 d ATRA exposure were driven by differential expression of 29 histone-encoding genes concentration Transcriptome profiling to identify ATRA-responsive genes in human iPSC-derived endoderm for high-throughput point of departure analysis Katerine S. Saili, Todd J. Zurlinden, Todor Antonijevic, Imran Shah, Thomas B. Knudsen National Center for Computational Toxicology, U.S. Environmental Protection Agency, Office of Research and Development, Research Triangle Park, NC, USA Background Methods Results (pathway analysis) Summary and Conclusions References saili.katerine@epa.gov l 919-541-3871 • Toxicological tipping points occur at chemical concentrations that overwhelm a cell’s adaptive response [Shah et al. 2016] • Human iPSC-derived endodermal differentiation (endogenesis) is an in vitro platform for probing the developmental impacts of a toxicological tipping point • Endogenesis is critical for organs such as the stomach, intestine, colon, pancreas, liver, urinary bladder, trachea, lung, pharynx, thyroid, and parathyroid glands, visceral yolk sac • Retinoid signaling is critical for early development and directs morphogenesis, growth, and differentiation of the embryo including endogenesis via retinoic acid gradients • Epigenetic changes mediate retinoid-induced stem cell differentiation [Gudas. 2013] Log2(RPKM+1) values were used for differential gene expression analysis (ANOVA) and filtered (p < 0.00001; -2 > fold-change >2) KEGG Pathway Analysis: two highly enriched pathways (p < 0.0001) • Endodermal differentiation based on FOXA2 expression occurred between 6 h and 4 d • ATRA first suppressed differentiation at 0.1 µM (by 4 d) indicated by multiple pathways such as protein digestion and absorption that characterize the trajectories for tipping point analysis • ATRA increased the expression of 29 histone encoding genes by 4 d, suggesting DNA remodeling is a target process for tipping point analysis • Endogenesis may be a suitable ToxCast platform for molecular-level characterization of toxicological tipping points during cell differentiation • Shah, et al. 2016. Using ToxCast™ Data to Reconstruct Dynamic Cell State Trajectories and Estimate Toxicological Points of Departure. Environmental Health Perspectives. Jul;124(7) • Gudas. 2013. Retinoids induce stem cell differentiation via epigenetic changes. Semin Cell Dev Biol. Dec; 24(0) • The views expressed in this poster are those of the authors and may not reflect U.S. EPA policy Total RNA for each treatment (x2) Human fibroblast-derived iPSCs Low serum + Activin differentiating endoderm Exposures (Vala Sciences) Sample Collection (Vala Sciences) 0d 6h 1d 2d 3d 4d 5d 6d 7d 8d 0.1% DMSO 0.001 µM ATRA 0.01 µM ATRA 0.1 µM ATRA 1 µM ATRA 10 µM ATRA Cells (Allele Biotech) RNA Sequencing (Cofactor Genomics) Data Analysis (US EPA) Vitamin A  All-trans retinoic acid (ATRA) RAR/RXR Total RNA for each treatment (x2) Total RNA for each treatment (x2) >30 million single end reads 26011 genes (75339 transcripts) 0.1% DMSO 10 µM ATRA FOXA2 expression (purple) at 4 d ATRA reduced FOXA2 expression and other endoderm-specific biomarkers in a concentration- dependent manner at 4 d Results (epigenetics) KEGG pathway analysis of differentially expressed genes (6033) returned 8 highly enriched signaling pathways (p<0.0001) Tipping point FOXA2 fold-change (versus time-matched DMSO control) un: undifferentiating control Pathway name Conc. Time OBJECTIVE: Identify biomarkers for high content analysis of tipping points in a developmental context Ectodermal markers (FC versus 6h DMSO control) Endodermal markers (FC versus 6h DMSO control) Histone modifying enzymes Histones ATRA-responsive genes (p<0.00001, 2 < FC < -2) List of differentiation markers provided by Sid Hunter (EPA/NHEERL) Mean FC vs. time-matched DMSO control Mean FC vs. 6h DMSO control Mean FC vs. 6h DMSO control


Where do pale and yellow signals go in the Drosophila brain? Michael B. Reiser, Aljoscha Nern, Arthur Zhao, Kit Longden, Gerry Rubin, Miriam Flynn, Connor Laughland, Henrique Ludwig, and Ruchi Parekh Janelia Research campus, HHMI aMe12 Rh6-R8 (yellow ommatidia) Rh3-R7 (pale ommatidia) aMe12 Recently characterized aMe12 cells extend along pale columns in the medulla aMe12 cells reconstructed in FAFB SS-TEM volume aMe12 pR7 pR8 yR7 yR8 aMe12 cells identify pale and yellow columns for targeted reconstruction pR7 yR7 pR8 yR8 total % of total % of R7 % of R8 % of pale % of yellow Dm9 (6) 70 61 98 170 399 14.90 12.19 18.97 42.11 57.89 Dm8 (14) 226 151 0 0 377 14.08 35.07 0.00 59.95 40.05 Tm_unc (14) 59 17 50 46 172 6.43 7.07 6.79 63.37 36.63 Tm_OTu (6) 75 90 0 0 165 6.16 15.35 0.00 45.45 54.55 Tm5c (6) 8 4 20 110 142 5.30 1.12 9.20 19.72 80.28 Tm20 (4) 5 2 65 62 134 5.01 0.65 8.99 52.24 47.76 Mi4 (4) 0 0 56 57 113 4.22 0.00 8.00 49.56 50.44 Mi15 (4) 6 6 27 73 112 4.18 1.12 7.08 29.46 70.54 Dm2 (4) 21 3 55 15 94 3.51 2.23 4.95 80.85 19.15 ML1 (4) 0 0 56 38 94 3.51 0.00 6.65 59.57 40.43 yR7 (3) 0 3 0 80 83 3.10 0.28 5.66 0.00 100.00 pR7 (2) 0 0 68 0 68 2.54 0.00 4.81 100.00 0.00 Mi1 (4) 0 0 30 29 59 2.20 0.00 4.18 50.85 49.15 L3 (4) 18 15 11 12 56 2.09 3.07 1.63 51.79 48.21 Dm11 (2) 23 26 3 2 54 2.02 4.56 0.35 48.15 51.85 Tm5a (2) 0 52 0 0 52 1.94 4.84 0.00 0.00 100.00 Mt_unc (7) 2 11 11 18 42 1.57 1.21 2.05 30.95 69.05 Tm5b (3) 20 5 2 11 38 1.42 2.33 0.92 57.89 42.11 L1 (4) 2 1 19 14 36 1.34 0.28 2.34 58.33 41.67 Mi9 (4) 3 8 24 0 35 1.31 1.02 1.70 77.14 22.86 aMe12 (2) 4 0 24 0 28 1.05 0.37 1.70 100.00 0.00 yR8 (2) 0 27 0 0 27 1.01 2.51 0.00 0.00 100.00 C2 (3) 7 13 6 0 26 0.97 1.86 0.42 50.00 50.00 pR8 (2) 25 0 1 0 26 0.97 2.33 0.07 100.00 0.00 ML2b (3) 0 0 25 0 25 0.93 0.00 1.77 100.00 0.00 Dm_unc (3) 5 1 9 5 20 0.75 0.56 0.99 70.00 30.00 Mi10 (1) 0 0 0 6 6 0.22 0.00 0.42 0.00 100.00 C3 (1) 0 0 0 3 3 0.11 0.00 0.21 0.00 100.00 ML2a (1) 0 0 0 2 2 0.07 0.00 0.14 0.00 100.00 unknown (143) 30 51 43 65 189 7.06 7.53 7.64 38.62 61.38 total 579 496 660 753 2677 100.00 100.00 100.00 46.28 46.66 pre-synaptic cell types post-synaptic cell types Pale Yellow DRA Eye Lamina Medulla Lobula Lobula Plate 300 350 400 450 500 500 600 650 0 0.5 1 R7 Rh3 Rh4 Rh5 Rh6 Rh1 R8 R1-6 ) c ( ) a ( (d) (b) Spectral sensitivity Wavelength (nm) Rh5 Rh6 Rh3 Rh1 Rh1 Rh1 Rh1 Rh5 Rh1 Rh1 Rh3 Rh3 Rh4 Rh6 Motion vision Phototaxis Color vision Polarized light vision Current Opinion in Neurobiology Drosophila eye background Behnia & Desplan (2015) 50 µm lamina medulla central brain R7 Example R7 manually traced through ~2200 sections Full adult fly brain (Zheng, et al. 2018; temca2data.org) CATMAID environment (Saalfeld, Cardona, et al. 2009) Dm8 Dm2 Dm9 Dm11 TmOTu Tm5c Tm20 Mi4 Mi15 ML1 Mi1 L3 Tm5a Tm5b L1 Mi9 C2 C3 ML2a/b Examples of major R7 and R8 target neurons reconstructed to identification R7 R8 100 500 50 20 inputs (post-) R7 R8 outputs (pre-) synapse count 7-column FIBSEM 2-column pale TEM 2-column yellow TEM Comparing the synapse counts from our 4-column reconstruction to those of Janelia FlyEM’s 7-column FIBSEM (Takemura, et al. 2015). Surprisingly we find a similar number of synapses. A partial explanation is that the TEM volume contains the lamina and connections. 15.4% of input and 7.4% of output synapses are found outside of the medulla. In the 7-column reconstruction, only 57% of R8’s pre-synapse partners could be identified; 48% for R7’s partners. Connectivity summary •’Greedy’ approach to targeted connectome of R7/R8 shown to be practical; propose to use as test case for new analysis methods. •Unique identity for many Tm cells proving difficult only using morphology -- more extensive reconstruction and connectivity required. •Several candidate pale or yellow ‘specialist’ target neurons identified. Ongoing work to verify these and search for evidence of potential opponency mechanism. • What would you do next?


tub. Forschungsdatenmanagement Nachhaltigkeit Open Access Transparenz Transparenz Creative Commons Denickestr. 22 21073 Hamburg www.tub.tuhh.de Universitätsbibliothek TU Hamburg tub. Open Research Open Research Open Data Offenheit Open Infrastructure OER Forschen und Publizieren Open Research FDM Open Access CC-Lizenz Forschungsdatenmanagement Offene Lizenzen eLearning Open Data Offene Lizenzen Urheberrecht HOS HOOU Transparenz Open Research Open Infrastructure Open Science Forschen und PublizierenWissen Lehren und Lernen Offenheit Digitalisierung Nachhaltigkeit Forschungsinformation Open Access Repository Research Data Management Organizer Wissenschaftliches Arbeiten Hamburg Open Science Hamburg Open Online University Open Educational Resources Creative Commons tub.services Offenheit Transparenz Open Research Nachhaltigkeit Wissen Informationskompetenz Open Research Forschungsdaten Copyright Wissenschaftliches Arbeiten Open Data Offenheit Open Science Wissen Open Data RDMO OA-Publikationsfonds Nachhaltigkeit Wissen Open Access Offenheit openTUHH Forschungsdatenrepositorium Information und Beratung Collect Write Publish Wissen


v Citizen collectives for healthcare spread to nearby neighborhoods Kevin Wittenberg, Rense Corten performance Main result 1 Care collectives are spatially clustered, even while accounting for regional attributes Main result 2 Care collectives are not much better predicted than baseline. Political climate may be important Using residuals of a data-driven model, we estimate spatial correlation that cannot be attributed to underlying correlates of collective action. Methods Background Citizens increasingly organize (in)formal care services among themselves in residential communities. We investigate their emergence based on a theoretical frame of diffusion of behavior. To do so, we test whether these care collectives are geographically clustered, while accounting for spurious causes of clustering. Administrative data Machine learning The Contagion of Collective Action: The Spread of Citizen Collectives for Care in The Netherlands. Spatial statistics v Work in progress Test set performance of final model Cross-validated performance of various methods Moran’s I interpretation Overall Accuracy: 0.83 Recall: 0.28 Precision: 0.75 Results for H1 & H2 Joincount interpretation Hypothesis testing -1 1 Residual Presence of collective Absence of collective H1 Care collectives are positively spatially correlated H2 Residuals of care collectives are positively spatially correlated H3 Local spatial correlation is weaker across municipal borders Conclusion We are among the first studies to quantify the geographic dispersion of citizen collectives for care. We find support for the notion that citizen collectives can influence collective action in neighboring regions. We combined data- driven techniques and inferential statistics.


Self-Identifying Factors include.. Diversity is often measured in open source communities through surveys by factors such as: ●Geographic region (i.e. city, state, country, continent) ●Role in an organization (i.e. developers, managers, executives) ●Types of problems someone works on (i.e. design, front-end engineering) ●Experience level (i.e. number of months or years working with a project) ●Project community (i.e. Gophers are members of the Go community) Diversity is also been measured in open source communities by factors such as: Gender, Age, Sexual orientation, Race, Ethnicity Known Measurement Problems ●Survey Design1 ●Biased sampling ●Oversimpliﬁcation ●Overstating results Representation and counting people matters, but for whom do these kinds of statistic serve? Who does this data belong to? We must also consistently ask of our demographic collection and goals: ●Who does this serve? ●Who does this leave out? ●Who does this harm? ●Who does this expose? Patterns and Anti-Patterns when Measuring Diversity in Open Source amanda casari — amcasari@google.com References 1. "Writing Survey Questions", Pew Research Center, https://www.pewresearch.org/our-meth ods/u-s-surveys/writing-survey-questio ns/ 2. Data Feminism, Catherine D'Ignazio and Lauren F. Klein, (2020) 3. Golang Developer Survey, Go Community (2021). 4. Blaise Agüera y Arcas, Margaret Mitchell and Alexander Todorov, Physiognomy’s New Clothes, (2017). 5. Lockhart, J.W., King, M.M. & Munsch, C. Name-based demographic inference and the unequal distribution of misrecognition. Nat Hum Behav (2023). Open Source Programs Office opensource.google About this Project Google Open Source's mission is to bring the value of open source to Google and all of the resources of Google to open source. We believe that open source solves real-work problems for everyone. Because when we say everyone, we mean everyone. So, how do we know we are - enabling everyone? encouraging everyone? creating space for everyone? So...how do you measure "everyone"? We set specific goals. We measure against them. We aim to do even better. Pattern: Understand Your Intent + Design with Purpose 1. Diversity, equity, and inclusion programs may be centralized or decentralized, but DEI work is integrated into all Google human-centered programs 2. If your mission statement includes words like "everyone", "all", or "community", you will want to know how your work is meeting those goals Anti-Pattern: Tacking demographic questions onto any available mechanism 1. Relying on demographic questions from ad-hoc surveys can complicate survey design 2. Regularly scheduled data collection and transparent analysis demonstrate commitment to community goals Pattern: Collecting Data for Accountability 1. Be clear + transparent with your communities on your diversity goals 2. Explain why you ask speciﬁc questions + what you ﬁnd in plain language a. "These questions allow us to measure diversity in the community and highlight opportunities for outreach and growth." (Golang Developer Survey 2021) Anti-Pattern: Every Single Inferential Method assigning identity At Google, we don't use, recommend, or release products which algorithmically infer gender, race, age, sexual orientation, ethnicity … These inferential algorithmic methods continue to be debunked as pseudoscience, even when dressed in technology: 1. Physiognomy’s New Clothes, 20174 2. Name-based demographic inference and the unequal distribution of misrecognition, 20235 Measuring Diversity "Measuring diversity" speciﬁcally refers here to measuring differences in self-identifying factors that people use to conﬁrm themselves in relation to others. Using principles from biodiversity + ecosystem diversity, we focus on more than just individuals or ﬂattened proﬁles. ●"Nature abhors a vacuum" or "Nature ...umm...ﬁnds a way." We are not measuring in a vacuum, designing for monocultures, or assuming spherical cows. Pattern: Ask Well-Designed Questions (build with!) 1. Designing


Gravity measurements in the Moscow gravity network I. A. Oshchepkov1,3, R. A. Sermyagin1, A. A. Spesivtsev1, V. D. Yushkin1, A. V. Pozdnyakov1, A. A. Kovrov2, P. A. Yuzefovich2,4 1Center of Geodesy, Cartography and SDI (ex. TsNIIGAiK) 2Moscow State University of Geodesy and Cartography (MIIGAiK) 3ilya@geod.ru, 4pauzhas@gmail.com Introduction The metrological local precise gravity networks are created to calibrate relative gravimeters, to test absolute gravimeters (AG) and to improve methods of performing and processing gravity measurements. We report on the gravity measurements campaign in the Moscow Gravity Network in Spring 2015. The major goals of this campaign were to determine: • linear scale-factors for relative gravimeters, • gravity at all stations w.r.t. International Comparisons of Absolute Gravimeters (ICAG2013), • oﬀsets of the AG which did not participate in Russian–Finnish Comparisons of Absolute Gravimeters (RFCAG2013). 1. Network The Moscow Gravity Network consists of six stations (see Fig.1): TsNIIAGik, Zvenigorod, Krasnaya Pres- nya, Ledovo, Troitse Seltso, Mendeleevo. The largest gravity diﬀerence is about 52 mGal. All stations are permanent and have gravity values from absolute measurements which are usually repeated annualy. 46 −6 1 20 27 −52 −19 −7 −19 −7 TsNIIGAiK Troitse−Seltso Krasnaya Presnya Mendeleevo Zvenigorod Ledovo 20 km Figure 1: Moscow gravity network The absolute gravity measurements at TsNIIGAiK and Zvenigorod were performed by FG5x #221 (Finnish Geospatial Research Institute) in 2013 and gravity values there are known w.r.t. ICAG2013 in Walferdange (Luxembourg). The relative gravity measurements were planned so that each point was connected to the nearest three neighbors. There are 10 lines in the network. 2. Instruments and measurements Figure 2: All CG-5 gravimeters (left) and absolute gravimeter GBL-M (right) 2.1 Absolute gravity measurements The absolute gravity measurements were made with GBL-M #001 and #003 ﬁeld ballistic gravimeters[3] (see Fig.2). Both gravimeters did not participate in RFCAG2013 and have no correction w.r.t. ICAG2013. The measurements were performed at three points with each gravimeter with overlaps (see table below). S/N Stations GBL–M #001 TsNIIGAiK, Krasnaya Presnya, Mendeleevo GBL–M #003 TsNIIGAiK, Krasnaya Presnya, Ledovo All processing methods of the absolute gravity measurements were standard, except: • no self–attraction and diﬀraction corrections were applied, • Atlantida3.1 2014[2] was used to calculate tide correction. 2.2 Relative gravity measurements The relative gravity measurements campaign with the ﬁve Scintrex CG–5 • #41262, 41265, 40443 (of TsNIIGAiK) and • #41077, 4173 (of MIIGAiK) spring gravimeters took place in March–May 2015 at all stations of the network for the ﬁrst time. Here are some highlights: • diﬀerence method (from two to four runs per line); • 30 – 60 minutes of measurements per station, • thus no more than one line per day; • very careful transportation in the hands of the operators to minimize the errors due to tilts, shocks, weather conditions etc.; 3. Data processing All measurements were processed and adjusted with the newly and actively developed gravity processing software (not public yet) in Python programming language. 3.1 Vertical gravity gradients The gravity changes above the mark were approximated by second degree polynomial (see Table. 2) as discussed in [1], unadjusted measurements at three or four vertical levels were used as input data. Table 2: Polynomial approximation results: a and σa in µGal, b and σb in µGal m−1 Station a b σa σb σab TsNIIGAiK -349.59 15.59 3.50 2.48 -8.49 Zvenigorod -321.86 4.00 4.20 2.93 -12.13 Krasnaya Presnya -297.49 3.34 6.03 4.20 -24.82 Ledovo -320.91 7.83 6.06 4.29 -25.50 Troitse-Seltso -355.84 12.44 4.89 3.27 -15.56 Mendeleevo -255.04 -3.43 8.69 5.41 -46.24 3.2 Network adjustment The least–squares adjustment solutions were computed for three main cases: 1. RG+ICAG2013: relative measurem


Acknowledgements This project is supported by NCATS UL1TR001067, NLM 5T15LM007124 and 1R18 HS022641-01A1. Computer resources were provided by University of Utah Center for High Performance Computing. Contact: Ram Gouripeddi (ram.gouripeddi@utah.edu) Towards a Scalable Informatics Platform for Enhancing Accrual into Clinical Research Studies Utah CCTS Ramkiran Gouripeddi1,2, Mollie Cummins1,2,3, Elizabeth Lane4, Randy Madsen2, Ryan Butcher2, Jianyin Shao1, Katherine Sward1,2,3, Bernie LaSalle1,2, Robinson Singleton2,5, Julie Fritz3, Julio Facelli1,2 1Department of Biomedical Informatics, 2Center for Clinical and Translational Science, 3College of Nursing, 4College of Health, 5Department of Internal Medicine, University of Utah, Salt Lake City, Utah, USA Introduction • Issues with recruiting targeted number of participants in a timely manner often results in underpowered studies. • More than 60% of clinical studies fail to complete or requiring extensions due to enrollment issues. • Objective of this study is to develop and implement a scalable, organization wide platform to enhance accrual into clinical research studies. Methods • We are developing and evaluating an informatics platform: Utah Utility for Research Recruitment (U2R2) consisting of: • Semantic Matcher: An automated trial criterion to patient matching component that reports uncertainties associated with the match • Match Delivery: mechanisms to deliver lists of matched patients for different research and clinical settings • Structured Trial Criteria Capture. • As a first step, we limited the Semantic Matcher to utilize only structured data elements from the patient record and trial criteria. • We evaluated this first phase of U2R2 for an ongoing randomized trial with a target enrollment of 220 participants that compares two treatment strategies for managing back pain (physical therapy and usual care) for individuals consulting a non-surgical provider and symptomatic less than 90 days. • Study team notified of matching patients on a biweekly basis. Recruitment Processes within Utah Utility for Research Recruitment Current recruitment results for Back Pain study Discussion • Recruitment platforms can enhance potential participant identification. • Development and operationalization of such platforms requires attention to multiple issues involved with clinical research studies. • Unstructured data • Clinical eligibility criteria are usually unstructured and require human mediation and abstraction into discrete data elements for matching against patient records. • Key eligibility data are often embedded within text in the patient record. • Distributional semantic approaches, by leveraging this content, can identify potential participants for screening with more specificity. (See poster titled “Semantic Characterization of Clinical Trial Descriptions from ClinicalTrials.gov and Patient Notes from MIMIC-III”) • Structured capture of eligibility criteria could improve match performance. • Match Delivery: Workflows and thresholds for delivery of matched patients should consider characteristics of : (1) Research study, (2) Population, (3) Targeted enrollment, (4) Organizational and socio-technical issues surrounding clinical practice and research, (5) Standards for messaging (FHIR). • Utilizing user-centered design approaches and including clinicians, clinics, and patients in recruitment workflows could yield higher accrual indices. Example eligibility criteria and REDCap-based discrete capture form with embedded branching logic for metadata and modifiers


Inside-out quenching Outside-in quenching PS ridge −0.5 0.0 0.5 1.0 log EW(Hα) 0.002 0.004 0.006 L′(Hα) 1.5 2.0 2.5 3.0 D4000 ●Twice as frequent in globally passive systems than in globally active systems: → in line with simulations of ring formation via captures of small satellites ([1], [3]). ● α"emission in the rings of the globally active S0s is 4 times higher than in those globally passive, implying: →star formation feeds on the gas of the host, → in"plain rings. 0.0 0.5 1.0 log EW(Hα) 0.00025 0.00050 0.00075 L′(Hα) 1.8 2.0 2.2 D4000 PS PCA of spectral profiles of ~500 S0 MaNGA galaxies. Radial binning and stacking of spaxels to generate the spectral profile of each galaxy, i. e., the mean spectrum as a function of the galactocentric distance. Projection of the spectral profiles onto their first 2 principal components (~90% of the sample variance [4]). José L. Tous1, Jaime D. Perea2, José M. Solanes1, elena Domínguez Sánchez3 ¹Institut de Ciències del Cosmos, Universitat de Barcelona (ICCUB), ² Instituto de Astrofísica de Andalucía (IAA"CSIC) , ³ Instituto de Ciencias del Espacio (ICE"CSIC) The examination of the spatially resolved IFS maps in a sample of more than 500 lenticular (S0) galaxies drawn from the MaNGA survey has unveiled the existence of transient inner annular structures (⟨R⟩ 1 R ∼ 1 R e) betraying ongoing star formation in a good number of these objects. Activity gradients in these galaxies have been measured through a novel methodology based on the principal component analysis of their optical spectra averaged over bins of galactocentric radius. We find that the sign of these gradients is closely linked to the presence of rings in the spectral maps, which are specially conspicuous in the equivalent width of α"emission, EW( α), with a fractional abundance —22–37% depending on the strictness of ring identification— larger than that inferred from optical imaging. While the numbers of S0s with globally positive, negative, and flat activity gradients are comparable, star"forming rings are found almost exclusively in objects with a positive slope for which quenching proceeds inside"out, in good agreement with predictions from cosmological simulations studying S0 buildup. The assessment of these structures and the properties of the galaxies harboring them also indicates that the frequency of such rings increases with the mass of their hosts, that they feed mainly on the gas from the disks, that are more short"lived in galaxies with ongoing star formation, and that the local environment does not play a relevant role in their formation. From the present analysis, we conclude that the presence of rings in the EW of the α emission line is a common phenomenon among fully formed S0s, possibly associated with annular disk resonances driven by weakly disruptive mergers preferentially involving the more massive representatives of these galaxies and their smaller and closer satellites. – ACKNOWLEDGMENTS: Financial support from the Spanish state agency MCIN/AEI/10.13039/501100011033 and ERDF through the grants: PID2019–106027GB–C41, PID2019–106027GB–C43, PRE2020–091838 and PID2020"115098RJ"I00. 1 Put a ring on it: the origin of star-forming rings in S0 galaxies The closer to the PS in the PC1–PC2 plane, the weaker the activity (SF/AGN) [2]. Vectorization and quenching classification. Profiles are converted into vectors. Then, galaxies are classified according to the orientation of these vectors wrt the PS ridge. This orientation establishes how quenching proceeds in galaxies. 2 Uniform SF Uniform SF Star-forming rings are common in S0 galaxies (20% to 40%) and are almost exclusively found in galaxies with inside-out profiles. 3 Implications: → the gas hinders the formation of rings, → the captured satellites must be tiny galaxies. The ring fraction increases with stellar mass. No dependence with environment. 4 REFERENCES: [1] Deeley, S., Drinkwater, M. J., Sweet, S. M., et al. 2021, MNRAS, 508, 895, 2021MNRAS.508..895D; [2]


Correlation between electron shower energy and beam momentum Correlation between electron shower energy and beam momentum can be fitted as 𝑬𝒔𝒉𝒐𝒘𝒆𝒓= 𝒑𝟎+ 𝒑𝟏⋅𝑬𝒃𝒆𝒂𝒎a linear straight line, which can be influenced by the missing energy. *Preliminary result MC p0 = -0.0164 ± 0.001547 P1 = 0.9416 ± 0.0008942 The calibration data with 7 GeV beam momentum is removed due to its bad electron life time —Runs with 7 GeV beam —Runs with other beam energy Interested in the determination of electron shower energy and its resolution A typical example of electron shower in ProtoDUNE detector Electron shower event Reference: [1] B. Abi, R. Acciarri, M. A. Acero, M. Adamowski, C. Adams, et al.. The Single-Phase ProtoDUNE Technical Design Report; 2017 [2] Acciarri R, Adams C, An R, Aparicio A, Aponte S, Asaadi J et al.. Design and Construction of the MicroBooNE Detector; 2016 Linhui Gu for DUNE collaboration Electron Shower Energy Reconstruction for the ProtoDUNE experiment Analysis goal 2 Resolution calculation 4 Detection principle Neutrino interacts with the argon in the TPC, creating secondary particles Charged particles in the TPC excite and ionize the argon along their travel through the detector About 6000 electrons per mm are released from the ionization of argon These electrons are attracted by the anode plane where they are detected, while the much heavier positive ions move relatively slow in this field; Together with the scintillation light detected by a photon detection system, a 3D reconstruction of the interaction can be performed to identify the particles. Operating principle for liquid argon time projection chambers.[2] The beam of the tertiary particles was designed to cover the expected spectrum of particles from neutrino interactions in the DUNE detectors. Get shower charge Electron life time correction Missing energy correction Energy charge conversion Get shower energy Charge calculation 3 ∼0.1 GeV shift caused by missing energy for 1 GeV beam momentum events *Preliminary result Calibration run with beam energies: 0.3, 0.5, 1, 2, 3 and 6 GeV Beam momentum Beam momentum resolution measured as a function of momentum, which will directly contributes to shower charge resolution as the constant parameter *Preliminary result Energy resolution vs beam momentum: 𝝈𝑬 𝑬𝒔𝒉𝒐𝒘𝒆𝒓= 𝒂𝟐+ 𝒃 𝑬𝒃𝒆𝒂𝒎 𝟐 + 𝒄 𝑬𝒃𝒆𝒂𝒎 𝟐 Stochastic term of resolution: 𝟏 𝑬𝒔𝒉𝒐𝒘𝒆𝒓 a = 0.066 ± 0.0097 Constant term • From beam momentum b = 0.023 ± 0.0639 GeV Stochastic term • Contributed by missing energy • Represents the fluctuation in the ionization process c = 0.050 ± 0.0099 GeV Noise term • Fluctuation in upstream energy loss Energy resolution *Preliminary result Electron lifetime correction Impurities in liquid argon can capture ionization electrons, reducing the signal read on the wires 𝑸𝒄𝒐𝒍𝒍. This charge loss can be corrected by a function of a hit drift time 𝒕and the free electron lifetime 𝜏𝑒of the TPC: 𝑸𝒄𝒐𝒓𝒓= 𝑸𝒄𝒐𝒍𝒍⋅𝒆Τ 𝒕𝝉𝒆 Larger electron lifetime means better purity, so we have a goodness requirement: 𝜏𝑒>10ms Missing energy correction Missing energy is caused by ADC charge threshold (around 100 keV/tick), which will directly affect the charge calculation of the electron shower. In this work we estimate the missing energy based on MC simulated data. Introduction 1 ProtoDUNE experiment Structure of protoDUNE-SP detector[1] ProtoDUNE detector at CERN. There are two ProtoDUNE detectors, namely ProtoDUNE-SP and ProtoDUNE-DP(now VD), which are horizontal and vertical drift TPCs respectively. •The ProtoDUNE experiment is a full engineering prototype of the DUNE far detector, and took test beam data at CERN. • ProtoDUNE is the largest Liquid Argon Time Projection Chamber (LArTPC), which contains about 770 tons of liquid argon, with 420 tons in the active volume. • These engineering prototypes will test DUNE detector components and help establish construction procedures. ProtoDUNE-SP ProtoDUNE-DP(now VD) Average missing energy per event ∼56 MeV/event (1 GeV) Beam momentum 1 GeV


Mariano Lazzeri1; Ana Clara Ventura2,3 zzmariano17@gmail.com 1 Facultad de Ciencias de la Educación (FACE), Universidad Nacional del Comahue (UNCo) 2 “Estudios Culturales y Cognitivos”, GV al IPEHCS (CONICET-UNCo: sede CRUB). 3 Centro Regional Universitario Bariloche, Universidad Nacional del Comahue (UNCo) OBJETIVO I. Analizar el despliegue metacognitivo de niñes de primer grado durante la producción de un texto al inicio del año escolar, y durante su revisión seis meses más tarde, con la finalidad de comparar los procesos desplegados por un mismo niñe en ambas instancias (producción y revisión). Participaron 60 niñes de primer grado (SD = 3 meses; Min = 5,3; Max= 6,9). En conjunto con las docentes se seleccionaron 16 niñes que evidenciaran diferente rendimiento académico. Instrumento: Tarjeta de Identidad (Dockrell y Teubal, 2007) Procedimiento: Análisis mixtos; de grano fino y detallado de los ítems de todas las tarjetas de identidad (32). Cálculo de distribución de frecuencias y prueba chi cuadrado. PROCESOS METACOGNITIVOS DESPLEGADOS EN FASES DE PRODUCCIÓN Y REVISIÓN *Nota las siglas corresponden a: CP (conocimiento de la persona); CT (conocimiento de la tarea); CEs (conocimiento de las estrategias); Pl (planificación); M (monitoreo); C (control); E (evaluación); ME (monitoreo emocional-motivacional); CEm (control emocional-motivacional) Nuestros resultados evidencian que niñes de primer grado pueden revisar un texto de su propia autoría, al encontrar: I. Todo el arco de procesos metacognitivos tanto en la fase de producción (FP) como en la de revisión (FR). II. Diferencias significativas en cuanto al despliegue de los siguientes procesos: planificación, monitoreo, control, evaluación y, monitoreo y control emocional/motivacional en FR respecto de FP. En línea con lo anterior, se plantea la relevancia de incorporar actividades de revisión en los primeros años de la trayectoria escolar. Pensamos analizar cómo niñes de primer grado revisan un texto antes de comenzar a escribirlo (Calil y Myhill, 2020). Calil, E., & Myhill, D. (2020). Dialogue, erasure and spontaneous comments during textual composition: What students’ metalinguistic talk reveals about newly-literate writers’ understanding of revision. Linguistics and Education, 60, 1–15. https://doi.org/10.1016/j.linged.2020.100875 Dockrell, J. E., & Teubal, E. (2007). Distinguishing numeracy from literacy: evidence from children's early notations. In E. Teubal, J. E. Dockrell, & L. Tolchinsky (Eds.), Notational knowledge. Developmental and historical perspectives (pp.113-134). Rotterdam: Sense Publisher. Whitebread, D., Coltman, P., Pino Pasternak, D., Sangster, C., Grau, V., Bingham, S., Almeqdad, Q., & Demetriou, D. (2009). The development of two observational tools for assessing metacognition and self-regulated learning in young children. Metacognition and Learning, 4, 63–85. https://doi.org/10.1007/s11409-008-9033-1 Fase de Producción Fase de Revisión PROCESOS METACOGNITIVOS DE NIÑES DE PRIMER GRADO DURANTE LA PRODUCCIÓN Y REVISIÓN DE UN TEXTO SOBRE SÍ MISMOS Estudio realizado bajo una Beca EVC-CIN Res CE N° 1518/20 del primer autor con la dirección de la segunda autora. PROBLEMA ¿Despliegan niñes de primer grado distintos procesos metacognitivos al revisar un texto que al producirlo? ¿Qué diferencias pueden identificarse? METACOGNICIÓN (Whitebread et al 2009) ÁREAS PROCESOS EJEMPLOS (de este corpus) CONOCIMIENTO METACOGNITIVO PERSONAS FP: “No sé mucho leer pero estoy aprendiendo en mi casa” (Kayla)/ FR: “Yo sé que Castek empieza con la “A”” (Lorenzo). TAREAS FP: “Como la de mamá [refiere a la letra M]… es muy difícil” (Rocío. M)/ FR: “La G de gato y la de Gian Luca” (Gian Luca). ESTRATEGIAS FP: “Si no te acordás otro día le dices a mamá y ahí lo escribís” (Jassiel)/FR: La cabeza, la tengo que borrar porque la hice muy chiquitita (Jassiel). REGULACIÓN METACOGNITIVA PLANIFICACIÓN FP: “Me decís como voy a hacer que haga todo el Bariloche... Me podes ayudar haciendo


Phase change insulation for energy efficiency based on wax-halloysite composites Yafei Zhao, Suvhashis Thapa, Leland Weiss, Yuri Lvov Institute for Micromanufacturing, Louisiana Tech University, Ruston, LA 71270 Abstract Wax can be used as a phase change material in solar energy storage but has low thermal conductivity and cannot sustain shape at higher temperature (above 55 C). Introducing 50% halloysite clay nanotubes into wax yields a stable and homogenous phase change composite with thermal conductivity of 0.36 Wm-1K-1 and no leaking until 70 C (preserving layer-shape above the original wax melting point). Thermal conductivity of wax/halloysite/graphite (45/45/10%) composite showed a six-fold conductivity increase to 1.4 Wm-1K-1 compared to pure wax and had no liquid wax leakage until 81 C. Wax/halloysite/graphite/carbon nanotubes (45/45/5/10%) composite show thermal conductivity of 0.85 Wm-1K-1 while maintaining the original shape until 91 C. Vectorial thermal energy transfer for double layers of different phase change materials demonstrated heat flux difference in the opposite directions differed by 25%. This variance in layer conductivity allows for smart building roof insulators with increased absorption during hot weather but limited thermal losses during periods of cooler temperatures. The new wax-nanoclay composite is a promising heat storage material due to good heat capacity, high thermal conductivity and ability to preserve its shape during wax melting. Conclusions (1)Halloysite can offer as a support to maintain wax shape during heat absorption and phase change even when the temperature of the PCM exceeds the melting point. (2)Halloysite can also improve the thermal conductivity of wax. Shape stabilized temperature and thermal conductivity can be further enhanced by adding graphite or carbon nanotubes to wax/halloysite composite. (3)More than one phase change materials with different thermal conductivity and melting point can be combined to improve the performance of phase chage storage, and this design has a potential application in the building roof. Acknowledgements Support with NSF-1029147 and NSF- EPS1003897 grants are acknowledged. Authors are thankful to Applied Minerals Inc., USA for providing halloysite samples. Wax and Halloysite nanotubes Wax as a phase change material (PCM) Advantages: High heat of fusion (150-250 J/g), Appropriate melting temperature (51-53 C), Chemically inert and stable and noncorrosive and nontoxic Disadvantages: Low thermal conductivity (~0.25 W/ mK) For further information contact ylvov@latech.edu Experimental setup The composites were prepared by reflux blending and impregnation method. Wax was melted at 80 C and dissolved in 80 mL isooctane solution to form a homogenous solution under continuous stirring for 10 min. Halloysite (and graphite and carbon nanotubes) was added to the above solution and the resulting mixture was than stirred and refluxed under 80 C for 3 hours. Finally, the composites were sealed in a vacuum drier at room temperature for 3 days to evaporate isooctane solvent from the mixture. Results and discussion Table 2. Thermal conducivity of composites Figure 3. Images of pure wax (a) wax/halloysite (b) wax/graphite (c) wax/halloysite/graphite (d) wax/halloysite/graphite/carbon nanotubes at different temperature Reference [1] Y.M. Lvov, D.G. Shchukin, H. Möhwald, R.R. Price, ACS Nano. 2 (2008), 814-820. [2] E. Abdullayev, K. Sakakibara, K. Okamoto, W. Wei, K. Ariga, Y. Lvov, ACS Appl. Mater. Interface 3 (2011), 4040-4046. [3] B. Zhang, J. Zhang, et al. Solar Energy 86 (2012) 1142–1148. [4] M. Xiao, B. Feng, K. Gong. Energy conver. Manag.43 (2002) 103-108. [5] H. Kao, M. Li, X. Lv, J. Tan. J. Therm. Anal. Calorim .107 (2012) 299-303. W:HNT:G:CNT (w%) Melting Transition T (ºC) Melting point T (ºC) Melting latent heat ∆H (J/g) Total latent heat ∆H (J/g) 100:0:0:0 47.5 52.3 140.5 171.0 50:50:0:0 47.4 53.3 89.9 107.7 45:45:10:0 47.1 52.9 57.4 68.4 45:45:5:


Evidence for Early Formation of the Most Compact Quiescent Galaxies at High Redshift Using Deep Hubble Grism Data Vince Estrada-Carpenter1 NASA FINESST Future Investigator Casey Papovich1, Iva Momcheva2, Gabe Brammer3, Raymond Simons2 and the CLEAR Collaboration The origin of the correlations between mass, morphology, and formation history in galaxies is diﬃcult to deﬁne, primarily due to uncertainties in galaxy star-formation histories, which are better constrained for higher redshift galaxies. Here we use a forward modeling technique on deep HST grism data (a method which can be applied to grism data from the Nancy Grace Roman Space Telescope) from the CLEAR (CANDELS Lyα Emission at Reionization) survey. We derive constraints on the formation and quenching timescales of quiescent galaxies at 0.7 < z < 2.5 using “non-parametric” star-formation histories. The galaxy formation redshifts, z 50 (deﬁned as the point where they formed 50% of their stellar mass) range from z50 ~ 2 (shortly prior to the observed epoch) up to z50 ~ 5-8. We ﬁnd that early formation redshifts are correlated with high stellar-mass surface densities, log(Σ1) > 10.25, where Σ1 is the stellar mass within 1 proper kpc. Quiescent galaxies with the highest stellar-mass surface density, log(Σ1) > 10.25, show a minimum formation redshift: all such objects in our sample have z50 > 2.9. Quiescent galaxies with lower surface density show a range of formation epochs (z50 ~ 1.5 - 8), implying these galaxies experienced a range of formation and assembly histories. We argue that the surface density threshold log(Σ1) > 10.25 uniquely identiﬁes galaxies that formed in the ﬁrst few Gyr after the big bang, and discuss the implications this has for galaxy formation models. Abstract Acknowledgment References VEC acknowledges support from the NASA Headquarters under the Future Investigators in NASA Earth and Space Science and Technology (FINESST) award 19-ASTRO19-0122, as well as support from the Hagler Institute for Advanced Study at Texas A&M University. Based on observations made with the NASA/ESA Hubble Space Telescope, obtained at the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS 5-26555. These observations are associated with program #14227. Support for program #14227 was provided by NASA through a grant from the Space Telescope Science Institute, which is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS 5-26555. The Most Compact Galaxies Formed at High Redshift Our SFHs Span Many Shapes Extended Galaxies Have Extended Star-Formation ●UVJ selected quiescent galaxies at 0.6 < z < 3.5 ●Selection criteria from Whitaker et al. 2011, prevents selection of dusty star-forming galaxies ●Selected ~ 100 massive quiescent galaxies We Select Quiescent Galaxies From CLEAR ●Main result of this work shows that galaxies with higher ∑1 measurements have higher formation redshifts ●The most compact galaxies all have z50 > 2.9 creating an envelope at the highest ∑1 , suggesting that the most compact galaxies can not form at lower redshifts ●The ﬁgures on the right show that this relationship monotonically rises and the standard deviation drops ●These results suggest that the most compact galaxies get their compact morphology from having formed in the early universe, when the universe was more dense ●Data: WFC3 G102 (CLEAR), WFC3 G141 (3D-HST, Momcheva et al 2016), photometry (3D-HST catalog, Skelton 2014) ●Fit all data simultaneously using forward modeling technique outlined in Estrada-Carpenter et al. 2019 and non-parametric star-formation histories (SFHs) from Leja et al. 19 ●SFHs (bottom panel in each subplot) span many shapes ○Use SFHs to measure z50 (formation redshift - when galaxy formed half its mass) We Quantify “Compactness” with ∑ ●Use ∑1 (stellar-mass surface density within 1 pkpc) to quantify how compact a galaxy is ●Being a in


Building an ontology of logic definitions for groups of biological organisms to enable data integration Gaurav Vaidya1, Hilmar Lapp2, Nico Cellinese1 1 University of Florida and Florida Museum of Natural History 2 Duke University All organisms are related to each other: an example with alligators Biologists visualize hypotheses of how organisms are related to each other using phylogenetic trees. For example, the following phylogenetic tree includes the hypothesis that alligators (Alligatoroidea) and crocodiles (Crocodylidae) are more closely related to each other than either are to gavials (Gavialis gangeticus). Fig 3. Lineages on the Open Tree of Life synthetic phylogenetic tree as visualized in [6]. Acknowledgments The Phyloreferencing project is funded by the US NSF through collaborative grants DBI- 1458484 (HL) and DBI-1458604 (NC). GV’s attendance of US2TS was funded by the US2TS Travel Grant. References 1.Brochu (2003) Ann Rev Earth Plan Sci 2003 31:1, 357-397. https://doi.org/10.1146/annurev.earth.31.100901.141308 2.Prosdocimi et al. (2009) Evol Bioinform Online. 5:47–66. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2747124/ 3.de Queiroz and Gauthier (1990) Syst Biol 39(4):307–322. https://doi.org/10.2307/2992353 4.Carral et al. (2017) Arxiv 1710.05096 https://arxiv.org/abs/1710.05096 5.Musen, M.A.ACM SIG AI 1(4) http://dx.doi.org/10.1145/2557001.25757003 6.Hinchliff et al. (2015) Proc Nat Acad Sci 112.41 (2015): 12764-12769. https://doi.org/10.1073/pnas.1423041112 Finding a clade on the Open Tree of Life The Open Tree of Life [6] synthesizes information from 987 phylogenies and several taxonomic resources to provide a draft hypothesis of evolutionary relationships between 2,640,941 taxa. Resolving clade definitions on the Open Tree of Life would facilitate navigation. Phylogenetic clade definitions identify groups of organisms unambiguously Phylogenetic clade definitions [3] define groups in terms of their ancestral relationships by specifying biological groups that must be either included in or excluded from the clade on a particular phylogenetic tree: Fig 1. Phylogenetic tree of alligators, crocodiles and caimans from Brochu 2003 [1]. Alligatorinae Alligator mississippiensis and all crocodylians closer to it than to Caiman crocodilus. Brevirostres Last common ancestor of Alligator mississippiensis and Crocodylus niloticus and all of its descendents. How can we translate a definition based on ancestral relationships into an OWL restriction expression? We can use OWL Property Chains as used in [4] to create clade definitions in OWL to traverse phylogenetic trees: - includes TU: has Descendant o represents TU - excludes TU: has Sibling o has Descendant o represents TU Node Alligator mississippiensis represents TU some (Has Name some (Zoological name and Has Scientific Name value "Alligator mississippiensis")) Node Crocodylus niloticus represents TU some (Has Name some (Zoological name and Has Scientific Name value "Crocodylus niloticus")) Phylogenetic trees can be represented in OWL using CDAO The Comparative Data Analysis Ontology (CDAO) [2] allows phylogenetic trees to be represented as an ontology in the Web Ontology Language (OWL). Alligator mississippiensis Caiman crocodilus Crocodylus niloticus Gavialis gangeticus Alligatoridae Brevirostres Crocodylia has Child Transitive as has Descendant has Sibling Symmetrical Note that nodes on this phylogenetic tree represent biological groups (taxa). We can relate these nodes to taxonomic names using the represents TU property from CDAO [2]. Name OWL restriction Nodes matched Alligatorinae includes TU some Alligator mississippiensis Alligator mississippiensis, Alligatoridae, Brevirostres, Crocodylia and excludes TU some Caiman crocodilus Alligator mississippiensis, Crocodylus niloticus, Gavialis gangeticus Name OWL restriction Nodes matched Brevirostres has Child some Brevirostres (includes TU some Alligator mississippiensis Alligator mississippiensis, Alligatoridae, Breviro


0.004 * * * * * * 95 * 97 * * * 93 * * * 68 * * * * * 98 * Epipactis microphylla Neottia ovata Neottia pinetorum Tropidia polystachya Neottia cordata Palmorchis trilobulata Epipactis purpurata Epipactis palustris Epipactis mairei1 Palmorchis pabstii Cephalanthera damasonium Neottia japonica Epipactis helleborine Russia Cephalanthera longifolia Diceratostele gabonensis Epipactis mairei Epipactis helleborine- White Epipactis gigantea Epipactis atrorubens Cephalanthera longibracteata Neottia suzukii Epipactis albensis Neottia fugongensis Cephalanthera rubra Epipactis veratrifolia Limodorum abortivum Epipactis helleborine-Green Neottia japonica1 E. helleborine species complex Mycoparasitism does not correlate with changes in the plastid genome in the orchid Epipactis helleborine Janice Valencia-D.¹, Kierstin Lipe¹, Kurt Neubig¹, W. Mark Whitten² ¹Plant Biology Department, Southern Illinois University, Carbondale, Illinois, 62901 ²Florida Museum of Natural History, University of Florida, P.O. Box 117800, Gainesville, FL 32611 Introduction Epipactis helleborine L.(Crantz) is a mixotrophic orchid that can obtain its carbon supplies in different ways that range from autotrophism to mycoheterotrophism (Fig. 1). Full mycoheterotrophism (=mycoparasitism) is associated with a drastic reduction in the chloroplast (plastid) genome due to the loss of genes related to photosynthetic pathways. However, this has not been shown in mixotrophic species. To determine if achlorophyllous individuals of Epipactis helleborine have alterations in their photosynthesis-related genes, we sequenced and assembled plastid genomes for green and albino plants. Figure 1. Jacquemyn & Merckx´s continuum trophic model (2019). A. E. helleborine albino form, a full mycoheterotroph (Photo: Damon Tighe). B. E. helleborine green form, a partial mycoheterotroph (Photo: Roh Curtis) 1. Green and albino forms of E. helleborine were collected in Virginia, Giles County, Mountain Lake Biological Station. DNA was extracted using a CTAB method (Doyle & Doyle, 1987), followed by a silica purification column step (Neubig et al., 2014). Sequencing was performed on an Illumina HiSeqX. The poorly assembled region at trnC- ACA intron was improved by adding sequences from PCR. 2. Plastid genomes were assembled in Geneious 10.2.3. Annotations were made using CHLOROBOX and were manually curated. 3. A Maximum Likelihood analysis of the Neottieae tribe, to which Epipactis belongs, was performed using coding regions and rRNAs from our two samples, three unpublished orchid plastid genomes, and 23 more downloaded from GenBank. For the E. helleborine species complex, the full plastid genome was compared to assess substitutions per site and hotspots. • The plastid genome of both forms of E. helleborine consisted of a single, circular DNA sequence, in the typical quadripartite structure (Fig. 2). • The plastid genome of E. helleborine contains all gene regions expected in an autotrophic orchid, including complete and functional ndh genes, which are the first group of genes that tend to be lost in taxa that exhibit some transition to partial or full mycoheterotrophy. • There was no evidence of mutations in coding regions in the plastid genome of the albino plant, which suggests that this condition is regulated by nuclear or mitochondrial genes in this species. • Among the widespread E. helleborine, numerous varieties, subspecies and forms have been described due to the high plasticity in vegetative morphology and floral size and color. A broad taxonomic revision is needed to fully understand the morphological breadth of the species complex, considering the genetic variation. • We identify 4 hotspots in the plastid genome and 141 sites that can be useful to identify phylogenetic groups inside the E. helleborine complex. These results are relevant for population genetic studies and for determining evolutionary dynamics like foundational effect in the North American individuals, inbreeding and fluct


Development of the Self-Archiving System in the Social Science Japan Data Archive 1Institute for Social Sciences, The University of Tokyo; ssjda@iss.u-tokyo.ac.jp Megumi Ikeda1, Nobutada Yokouchi1 & Satoshi Miwa1 Introduction Statistics of SSJDA Self-Archiving System of the Social SSJDA Visit our website! Challenges of our Self-Archiving Systems 0 200 400 600 800 1000 1200 1400 1600 0 500 1000 1500 2000 2500 3000 1998199920002001200220032004200520062007200820092010201120122013201420152016201720182019202020212022 Cumulative Accessible Date Sets (left scale) New Accessible Date Sets (left scale) Usage Applications （right scale） n The University of Tokyo, Institute of Social Science, Center for Social Research and Data Archives (CSRDA) created the Social Science Japan Data Archive (SSJDA) to support empirical research in social sciences in Japan and has been disseminating raw data since April 1998. n The collection and storage have been smooth. Over 2,400 data sets have been put into the public domain. n Our recent challenge is that we have been unable to keep up with the increasing volume of deposits. Therefore, SSJDA built a self-archiving system in February 2023. nNumber of applications: 1,410 (75 applications from international researchers and graduate students around the world) nThe total number of data provisions is 23,800. Data Depositors SSJDA STAFF ・Management of Depositor's Personal Information ・Prepare Data for Public Use Data Users SSJDA Direct ・Online Search System ・Online Download System ・Online Data Analysis System Registration and Updating user information Information of Deposited Data Usage Approval Usage Count Contact SSJDA Message from SSJDA User Account Page ・Searching for Data ・Applying for Data Usage ・Downloading Data ・Reporting Data Usage Depositors Account Page The Shift to Self -Management Search/Apply Aprove/Provide Use data for One-year Remind user to submit data usage report Submit publications Link data and publication n What can depositors do with the Self-Archiving System? n Register raw data n Select conditions for data n Create metadata n Check the list of applications for use, etc n Status of Self-Archive System Utilization n Of the 32 deposited, 21 have been self- archived (as of April 2023) n Depositors commented on the "convenience" of the system. n The lack of depositors creating metadata is a serious problem: many depositors need more experience creating FAIR metadata, and metadata creation is time-consuming, only one metadata currently created n The manual is already available. However, we need to show how to create metadata n To create benefits, such as shortening the time between deposit and release if they register metadata n Use AI to help create metadata n Many people deposit data the previous way n More promotion needs to be done that the self-archiving system has been created. n We need to inform depositors about the benefits of the self-archiving system (e.g., no need to contact CSRDA, no need to submit a deposit form). Image of Self Archiving Stem Overview of SSJDA


Synchrotronsignatures fromthecosmic-raydrivendynamo Dominik Wólta´nski, Mateusz Ogrodnik & Michał Hanasz Centre for Astronomy, Nicolaus Copernicus University, Toru´n, Poland Abstract We investigate synchrotron emission of galactic interstellar medium with the aid of a new Cosmic Ray Energy Spectrum Module (CRESP) for modeling energy-dependent transport of CR electrons. The module solves the Focker-Planck equation for a piece-wise power-law distribution function, with synchrotron and adiabatic cooling effects taken into account together with diffusive and advective propagation of CR electrons on an Eulerian grid. To demonstrate capabilities of the new module we perform numerical simulations of the CR electron spectrum in a galaxy similar to the Milky Way. The simulation results allow us to construct synthetic radio-maps of synchrotron radio-emission. Motivation Numerical studies of dynamical effects of cosmic rays in galaxies, including magnetic ﬁeld ampli- ﬁcation and galactic winds relying on a single- component propagation of CRs (Schlickeiser & Lerche, 1985), demonstrated formation of X-shaped magnetic ﬁelds in galactic halos (Hanasz, Wólta´nski & Kowalik, 2009) as well as magnetic arms (Beck, 2016) (Kulpa-Dybeł et al., 2011). This simpliﬁed treatment of CRs does not enable, however, to model galactic synchrotron emission with precision sufﬁ- cient to confront CR propagation models with the wealth of data coming from radio observations. In this contribution we demonstrate that coupling the MHD system of equations with the CRESP solver of Focker-Planck equation, within PIERNIK code (see poster by Mateusz Ogrodnik et al), makes it pos- sible to model evolution of the CR electron spectrum, and thereby to construct synthetic radio-maps of po- larized synchrotron emission from disk galaxies. ISM model with CR protons and electrons We assume that magnetized galactic interstellar medium (ISM) is stratiﬁed by vertical gravity of the stellar and dark matter components. The initial dis- tribution of interstellar gas and galactic gravity fol- low the model of the Milky Way by Ferriere (1998), while regular initial magnetic ﬁeld is assumed to be purely toroidal and its strength corresponds to pressure equilibrium with thermal gas. The basic characteristics of the model are similar to the global model of CR-driven dynamo by Hanasz, Wólta´nski & Kowalik (2009), except that initial magnetic ﬁeld is currently set near energy equipartition with thermal gas. The thermal gas component is described by the stan- dard set of MHD equations. We assume that super- novae (SNe) explode randomly in the disk and that 10% of SN explosion energy is converted to energy of CRs consisting of CR protons and electrons, while the thermal energy output from SNe is neglected. We assume that CR protons diffuse anizotropically along magnetic ﬁeld lines and are dynamically cou- pled to the thermal gas. We assume an Eulerian grid of 384x384x192 cells spanning Lx × Ly × Lz = 76 kpc×76 kpc×38 kpc in x, y, z directions, extended with the CR electron spectrum distributed over 16 bins spanning the energy range of 10 −105mec2. Acknowledgements This work has been supported by the (Polish) National Science Centre through the grant No. 2015/19/ST9/02959. Calculations were carried out at the Academic Computer Centre in Gda´nsk. CR electron spectrum and synchrotron emission We assume also that CR electrons are represented by a piece-wise power-law distribution function and are described by the Focker-Planck equation (Miniati, 2001, see poster by Mateusz Ogrodnik et al.). We take into account advection, energy-dependent (D ∝p0.5), magnetic ﬁeld-aligned diffusion as well as adiabatic and synchrotron cooling effects in dynamical evolution of CR electrons in the wind driven by CR protons. We construct synthetic radio-maps of polarized synchrotron emission of CR electrons by integration of Stokes parameters I, U and Q along the line of sight, with Faraday rotation taken into accoun


Presented at the Eighth International Congress on Peer Review and Scientific Publication (September 10–12, 2017; Chicago, USA) Abstract Objective: The Guidance for Developers of Health Research Reporting Guidelines recommend multidisciplinary stakeholder involvement, transparent and complete reporting, and updating guidelines based on feedback. Developers are accountable for stakeholder engagement, but how broad and meaningful is such engagement? Our objective was to provide empirical feedback to developers by investigating (1) the involvement of those ultimately affected by guidelines (eg, patients and carers) and regular end users of guidelines (eg, publication professionals), and (2) the transparency and completeness of reporting stakeholder involvement. Design: For this prospective study, conducted from September 2016 to January 2017, we included every reporting guideline for the main study types, as listed on the EQUATOR Network website. We pilot-tested a standardized data collection spreadsheet to extract data from the corresponding guideline publications. We quantified patient, carer, and publication professional involvement and used statisticians (listed as stakeholders in the Guidelines) as a control group. We assessed reporting transparency and completeness using the AGREE Reporting Checklist for documenting stakeholder involvement. For qualitative insights, we interviewed leaders from nonprofit, international, patient advocacy (International Alliance of Patients’ Organizations [IAPO]) and publication professional (Global Alliance of Publication Professionals [GAPP]) organizations. Results: Of the 33 guideline publications, the mean (SD) number of authors was 9 (SD 5.7, min 3, max 30) (median, 7; IQR, 5-11) and the mean (SD) number of working group members was 45 (SD 38.4, min 5, max 147) (median, 30; IQR, 23-43). Statisticians were authors for 24% (8/33) of the publications and were working group members for 15% (5/33). Patients, carers, and publication professionals were rarely identified, either as authors (0, 0, and 0, respectively) or working group members (0, 1 [3%], and 0, respectively). Reporting stakeholder involvement was deficient (eg, for statistician involvement, only 25% of publications met AGREE Recommendations). Leaders from IAPO and GAPP were not aware of having been invited to participate in developing guidelines, but thought that their stakeholders could provide unique and important insights. They encourage guideline developers to contact them to facilitate meaningful involvement. Conclusions: Guideline developers have rarely involved stakeholders affected by guidelines (patients, carers) or those regularly using guidelines (publication professionals) in the development process. The involvement of these key stakeholders could enhance the credibility, dissemination, and use of guidelines. If patients, carers, and publication professionals were represented by other stakeholders (which is not ideal given potential conflicts of interest), this was not documented; readers do not know who represented whom. The transparency and completeness of reporting of stakeholder involvement should be improved. Transparency and Completeness in the Reporting of Stakeholder Involvement in the Development and Reporting of Research Reporting Guidelines Karen L. Woolley PhD CMPP,1-3 Serina Stretton PhD CMPP,4 Lauri Arnstein MBBS5 1ProScribe KK Envision Pharma Group, Tokyo, Japan; 2University of Queensland, Brisbane, Queensland, Australia; 3University of the Sunshine Coast, Maroochydore DC, Queensland, Australia (karen.woolley@envisionpharmagroup.com); 4ProScribe Envision Pharma Group, Sydney, New South Wales, Australia; 5Evidence Envision Pharma Group, London, UK Acknowledgments and disclosures • No external funds were used for this research study. All authors are employees and shareholders of Envision Pharma Group and members of not-for-profit associations supporting ethical publication practices. KLW is a shareholder of


I II III IV V VI VII VIII IX X XI XII Ian Feb Mar Apr Mai Iun Iul Aug Sep Oct Noi Dec CICLU BIOLOGIC ANUAL Parthenocissus quinquefolia SPECII CU ASPECT SIMILAR CUM O RECUNOAȘTEM? Familia Vitaceae, Clasa Magnoliopsida Flori: dispuse în inﬂorescențe compuse, pe pediceli de 3-7 cm lungime. Frunze: palmat-compuse cu 5 foliole eliptice pânăla oblongi, de 5-12 cm lungime, acuminate, cu baza cuneată, distanțat și acut serate (margini dințate), lucioase și colorate în verde-închis pe fața superioară și mai deschis și lucioase pe cea inferioară. Fruct - bace sferice de culoare negru- albăstrui (0,8 cm diametru), ﬁecare conținând 3-4 semințe cordiforme (în formă de inimă). - prezintă rădăcini aeriene; - cârcei cu 5-12 ramiﬁcații terminate cu discuri adezive; - frunzele pe fața inferioară sunt glauce (verde-albăstrii); - ﬂorile sunt grupate într-un panicul terminal. Parthenocissus inserta Viță de Canada Habitat Fructe 6-18 cm Frunză 1 2 3 4 5 foliole Floare Liană, cu cârcei care au 3-5 ramiﬁcații răsucite, purpurii, de regulă fără discuri aderente. Înălțime: <20 m Inﬂorescențe


1. Objetivos del Proyecto En junio de 2021, se formalizó un acuerdo de colaboración entre el Planetario de Pamplona y varios Institutos de Edu- cación Secundaria (IES) en Navarra con el objetivo de promover el "Bachillerato de Investigación". Esta iniciativa educativa in- cluyó a los IES de Barañáin, Plaza de la Cruz, Ribera del Arga y Valle del Ebro, ofreciendo a los estudiantes una opción educa- tiva orientada a profundizar en métodos de investigación y análisis de problemas cientíﬁcos. El proyecto se centró en la construcción de una réplica del rover Perseverance y en la in- vestigación del entorno marciano, siguiendo una metodología de trabajo inspirada en la NASA. El rover Perseverance en los Bachilleratos de Investigación Ordóñez-Etxeberria, I., Planetario de Pamplona, (i.ordonez@pamplonetario.org), 2. Desarrollo del Proyecto Durante el curso 2021/2022, doce estudiantes de los cuatro institutos participaron en la construcción del rover y en investi- gaciones relacionadas con Marte. Estos estudiantes, reparti- dos equitativamente entre chicos y chicas, se reunieron men- sualmente para compartir avances y enfrentar obstáculos en la fabricación de las piezas asignadas a cada instituto. Además, exploraron temas como la meteorología, la geología, los cráteres de impacto y las tormentas de polvo en Marte, utili- zando datos de misiones espaciales y repositorios públicos del Planetary Data System (PDS). 3. Continuación y Nuevas Fases del Proyecto El proyecto continuó en el curso 2022/2023, con los estudi- antes avanzando en sus investigaciones y defendiendo sus proyectos a inicios del año. Una nueva fase del proyecto fue lanzada, centrada en la implementación de un brazo robótico para el rover. Esta fase contó con la participación de cuatro estudiantes del IES Plaza de la Cruz y del IES de Barañáin, quienes ﬁnalizarán la parte técnica y cientíﬁca del proyecto en 2024. Para el curso 2023/2024, el enfoque se trasladó a la meteorología marciana, proponiendo la construcción de esta- ciones meteorológicas que permitirán comparar datos de la Tierra y Marte. 4. Impacto y Reconocimientos El impacto del Bachillerato de Investigación ha sido signiﬁca- tivo, ampliándose con la participación de tres nuevos IES in- teresados en desarrollar proyectos junto con el Planetario de Pamplona. Esta expansión ha ampliado la cantidad de estudi- antes involucrados y ha fomentado una dinámica de trabajo colaborativa basada en reuniones mensuales. Estas sesiones permiten a los estudiantes compartir el proceso de construc- ción del rover y el avance de sus proyectos cientíﬁcos, pro- moviendo el intercambio de ideas y el aprendizaje mutuo. Además de los logros internos, el proyecto ha sido reconoci- do en diversos congresos y certámenes. Destacan vario pre- mios y participaciones como Tecnociencia 2023 y el Certamen Jóvenes Investigadores 2023. El proyecto ha sido galardona- dos por sus trabajos innovadores, demostrando el éxito y el potencial del programa para fomentar el pensamiento cientíﬁco y el trabajo investigador en la juventud.  Figura 1.- Desarrollo de la construcción de la réplica del rover Perseverance. Figura 2.- Recreación del modelo del rover en la superﬁcie de Marte. Figura 3.- Réplica del rover Perseverance construida en el proyecto, y expuesta en el Planetario de Pamplona.


Ruprecht-Karls-Universität Heidelberg Heidelberg Graduate School of Mathematical and Computational Methods for the Science Graduiertenkolleg 1114 Heidelberg Collaboratory for Image Processing Institute of Environmental Physics Novel Technique for 3D-Space Visualization of Concentration Fields of Air-Water Gas Transfer Darya Trofimova, Christine Kräuter und Bernd Jähne Heidelberg Collaboratory for Image Processing, Institute of Environmental Physics, University of Heidelberg, Germany Measuring Technique NH4+ NH3 Z C Water Phase Air Phase H+ NH4+ Z C H+ NH3 NH3 OH- ﬂuorescence z* z* znl NH3(aq) + H3O+ <-> NH4 ++H2O 1D Measurements: Experimental Set-up wind Basler acA2500 - 14gm Basler acA2500 - 14gm diode laser beam expander laser beam ﬂuorescent light from Pyranine air-sided camera water-sided camera glass window of the facility Wind-wave facility: Lenght = 1.75 m Width = 25 cm Depth = 20 cm Water volume ≈ 22 L Air volume ≈ 220 L Optical Set-up: ·Diode laser: wavelength - 445 nm ·Galileo expander ·Basler cameras: 2592×16 AOI 2.2×2.2 µm pixel size 350Hz frame rate ·Scheimpﬂug optics magniﬁcation: 0.43 air-side 0.32 water-side Fig.2. Sketch of the LIF experimental set-up for 1D measurements 1D Measurements: Results Fig.5. Experimental proﬁles for diﬀerent ammonia concentration with ﬁtted functions that comply with small-eddy model Fig.4. Experimental proﬁles for diﬀerent Pyranine concentration 3D Measurements: Experimental Set-up sCOM pco.edge sCOM pco.edge sCOM pco.edge 1.4 m 1.0 m LED ﬂuorescent water surface left camera middle camera right camera 3D Measurements: Results Conclusion: free stream velocity 1.8 m/s, friction velocity in water 3.7 cm/s free stream velocity 2.2 m/s, friction velocity in water 5.5 cm/s free stream velocity 2.6 m/s, friction velocity in water 6.4 cm/s Fig.3. Time-depth images of the temporal ﬂuctuations of ammonia in the water. The size of the vertical coordinte is 9.25 mm and horizontal coordinate - 13.75 sec. ammonia ﬂux ammonia ﬂux Fig.1. Sketch of NH3 invasion into the acid water for low and high concentrations of NH3. The main objective of modiﬁed LIF technique is to gain simpliﬁed shape of concentration ﬁelds of observed molecules. This can be achived by demanding the vertical concentra- tion proﬁles to have a steep decay at the certain depth resulting in binary ﬁelds. Fig.6. Sketch of the LIF experi- mental set-up for 3D measurements Fig.7. Example of estimated disparity maps. Lower disparity values correspond to higher distances. The images are 0.6s apart. The wave breaking event is visible. Large Annual Aeolotron Facility: Height = 2.41 m Width = 60 cm Water volume ≈ 18000 L Air volume ≈ 25000 L Optical Set-up: ·blue high power LEDs (wavelength - 455 nm) ·sCMOS cameras pco.edge 2160×2560 resolution 6.5×6.5 µm pixel size 100Hz frame rate Full Camera Calibration: estimation of intrinsic and extrinsic parameters for each camera Dense Reconstruction: every image was rectiﬁed to compute the disparity map using images from left and right camera. Block matching algorithm is used to ﬁnd correspondence between the images. - The experimental technique for mass boundary layer visualization was veriﬁed with the measurements of concentration proﬁles at the linear wind-wave facility with high spatial and temporal resolution. - The binary representation of concentration ﬁelds can be achieved with Pyranine concen- tration of 10-5 mol/L and initial pH value of 4. Varying ammonia concentrations in the air, the fraction of mass boundary layer can be investigated. - The technique allows observation of binary concentration ﬁelds at larger spatial scales. The invasion of ammonia was viewed with multiple camera set-up from underneath the fa- cility. The third dimension was reconstructed using stereo algorithm, beneﬁting the picture of a gas exchange through the air-water interface in four dimensions. NH4+ NH3 OH OH 7th International Symposium on Gas Transfer at Water Surfaces doi: 10.5281/zenodo.17672 7th Internati


Chemical Curation of Lantana camara Emissions across Native and Invaded Habitats Vipin Kumara, Sonali Chauhanb, Manish Kumara, Renu Kumaria, Suresh Babub, and Gitanjali Yadava a. Computational Biology Laboratory, National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi 110067, India b. School of Human Ecology, Lothian Road, Kashmere Gate 110006, Ambedkar University Delhi Lantana camara is a major threat to biodiversity in many parts of the globe. Chemical profiling is an effective tool to decipher this complex genus, to support ecology. This study compares chemical profiles of Lantana in native and invaded habitats. ØChemical curation led to accurate identification of compounds ØDistinct chemical profiles in native and invaded habitats ØGreater chemical diversity in Invaded Habitats. INTRODUCTION METHODOLOGY SUMMARY & PERSPECTIVES This work has been funded by NIPGR Core funds, Hamied Award from University of for support anCambridge and TIGR2ESS grant. Authors thank Ucam & NIPGR d Infrastructure. 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 (Z)-3-hexenol n-hexanol alpha-pinene sabinene beta-pinene myrcene alpha-phellandrene delta-3-carene o-cymene limonene 1,8-cineole (e)-beta- ocimene terpinolene linalool camphor alpha-copaene beta-bourbonene beta-cubebene beta-elememe beta-caryophyllene beta-copaene beta-guaiene alpha-humulene (e)-beta-farnesene alloaromadendrene t-muurolene germacrene D alpha-chamigrene alpha-farnesene delta-cadinene germacrene b spathulenol caryophyllene oxide % Area Compounds Chemical profile of Lantana camara var. in Native Range (Venezuela) 0 2 4 6 8 10 12 14 16 18 20 3-hexen-1-ol, (Z) α-pinene camphen Sabinene β-pinene 1-octen-3-Ol myrcene hexanol, ethyl α-phellandrene δ-3-carene α-terpinene p-cymene eucalyptol (E)-ocimene (Z)-ocimene 𝛾-terpinene 4-thujanol terpinolene linalool geranyl nitrile 2-p-menthen-1-ol camphor L-borneol 4-terpineol methyl salicylate α-terpineol 1-verbenone isobutenyl methyl ketone pentanoate,cis-3-hexenyl acetald bicyclogermacrene bicycloelemene δ-elemene α-cubebene cyclosativene α-copaene β-elemene elemene caryophyllene 𝛾-cadinene α-humulene neoalloocimene 𝛾-muurolene germacrene D bicyclogermacrene germacrene A cubebol sesquilavandulol (Z) davanone B davanone D nerolidol,E denderalasin spathulenol humulene oxide β-atlantone isoaromadendrene epoxide germacrene D-4-ol geranyl linalool isomer B guaia-3,9-dien-11-ol, cis- cedreanol lanceol,cis 6-epi-shyobunol farnesol, (2E,6Z) β-davanone-2ol β-chamigrene aromadendrene-4,10-diol thujopsan-2-α-ol longipinanol % Area Compounds Chemical profile of Lantana camara complex in Invaded Range (India) 11.98 25.10 0.59 53.60 3.96 3.57 % Area of Metabolome in Essential oil Alcohol Monoterpene Monoterpene alcohol Sesquiterpene Azulenic sesquiterpenol Not Classified 1.4 30.2 1 62.8 0.80.9 % Area of Metabolome in Essential oil Alcohol Monoterpene Monoterpene alcohol Sesquiterpene Azulenic sesquiterpenol Sesquiterpene oxide Foliar chemical profiles: Leaf samples of Lantana camara complex were collected in the month of March- April 2019 in Delhi (India). Hydro-distillation : For extraction of essential oil profiles using Clevenger assembly for 3-4 hours. Recovery: Pure essential oil yield via anhydrous sodium sulphate and diethylether Chemical profiling via GC-FID and GC-MS instrument Shimadzu QP2010. Bibliometry and Comparative Analysis: Chemical profiles obtained above were compared Venezuelan nativ datasets (Tesch, et. al. 2011). Statistics in R. REFERENCES 1. Love, A., Naik, D., Basak, S. K., Babu, S., Pathak, N., & Babu, C. R. (2009). Variability in foliar essential oils among different morphotypes of Lantana species complexes, and its taxonomic and ecological significance. Chemistry & biodiversity, 6(12), 2263- 2274. 2. Tesch, N. R., Mora, F., Rojas, L., Díaz, T., Velasco, J., Yánez, C., ... & Pasquale, S. (2011). Chemical composition and antibacterial activity of the essential oil of Lantana ØDifficulties to compare com


ENVIRONMENTAL STUDIES PROGRAM INFORMATION SYSTEM Conﬁgured, Not Coded: Rethinking How the General Public Discovers Environmental Science To better serve the public, the Bureau of Ocean Energy Management’s Environmental Studies Program (ESP) is rebuilding its science dissemination tool, the Environmental Studies Program Information System (ESPIS). The target audience for this tool are users with little knowledge of ocean science and the engaged government agencies. Built on the ArcGIS Online (AGOL) hub, the tool uses large format infographic cards to give users options to browse among four science topics: Physical, Chemical, Biological, and Social Sciences. Under each science topic, the user can select from a dozen or more themes. Under each theme, the user will ﬁnd a templated theme page that uses website widgets to search, sort, and ﬁlter curated lists of research projects, products, and applications. This buildable, sustainable interface enhances the public’s discovery of environmental science information. Title Theme pages have catchy titles that help users navigate from science topics to themes. Introduction When users ﬁrst navigate to the theme page, the header section presents them with this text element, which helps them glean the contents of the theme page. Region Bookmark The region widget enables users to quickly navigate to a predeﬁned map extent and reduces the number of cards in the map section to the ones from the bookmarked region Map The user interacts with tools to browse studies and related content. The user can select a map feature to highlight the corresponding card from a map section list. Studies List These are all the studies that contribute to the theme, and users can interact with all the map section tools to search, sort, and ﬁlter this list. Related Content List These derived products and webpages use a study product and/or combine parts of multiple study products to create value-added scientiﬁc information. When the user selects a related content card, the user actions function highlights the related study product(s). By: Jonathan Blythe (BOEM ESP) and Emily Sandrowicz (NV5, on contract to BOEM) The user can discover all the products resulting from ESP studies that contribute to a theme. If the user ﬁrst selected a card in the map section, then the related study product(s) will automatically highlight. Study Products The user can search, ﬁlter, and sort the list of documents published by the ESP Documents List The user interacts with the list of journal publications by using the search, ﬁlter, and sort tools. Publications List The recommender section includes one or more button widgets that link to similar theme pages, in case users ﬁnd this theme page useful, and so they do not have to navigate back to the science topic theme list in order to ﬁnd these other relevant theme pages Recommender Bureau of Ocean Energy Management Study Studies cards include the study title and program number elements. When the user selects a study card, the user actions function highlights the related study product.


Introduction & Question Material & Methods Conclusion Leandro B. C. Menezes1, Marcos R. Severgnini1, Diogo B. Provete1,2,3 leandrobc.menezes@gmail.com, marcosrafaelsevergnini@gmail.com, diogo.provete@ufms.br 1 - Institute of Biosciences, Federal University of Mato Grosso do Sul, Brazil; 2 - German Centre for Integrative Biodiversity Research, Leipzig, Germany; 3 - Gothenburg Global Biodiversity Centre, Göteborg, Sweden. Effects of urbanization on intra- and interspecific variability of frog metacommunities in two tropical cities Figure 1. Maps of Campo Grande (Mato Grosso do Sul, Brazil) and São José dos Campos (São Paulo, Brazil) with the 20 sampling sites (ponds), showing the change in land use between 1985 and 2022. Data from MapBiomas V. 8.0. Changes in land use Changes in ecoevolutionary dynamics 40 ponds Left side measurement (mm) of males (5 adults per species per pond) Relative leg length (residuals of a linear model) Relative head length and width (log) Body size Results & Discussion landscapemetrics São José dos Campos, SP, Brazil Campo Grande, MS, Brazil Impervious surface Pervious surface Urban pond Buffer 500m Figure 2. Urbanization gradient in Campo Grande (MS) and São José dos Campos (SP) with data from 2022. Urbanization in 2022 (%) Pond ID 1,051 individuals measured from 44 species and 5 families in both cities Rate of change in radiance (% 2022 - % 2012) 10 years Rate of change in green areas (% 2022 - % 1985) 37 years Rate of change in urbanization (% 2022 - % 1985) 37 years Urban Heat Island in 2022 (Cº) Building density in 2022 (number of buildings) buffer area Figure 6. Relative contribution of environmental variables to the components of phenotypic variation (intraspecific and turnover). The vertical black lines represent each environmental variable. In Campo Grande, urbanization and radiance rate, and building density had a significant effect on leg size at interspecific scale, and rate of change in green areas on body size at the intraspecific scale. In São José dos Campos, radiance and building density had a significant effect on head length and width at the intraspecific scale. São José dos Campos, SP, Brazil Campo Grande, MS, Brazil Figure 3. Standardized effect size (SES) of the T statistics for phenotypic traits at distinct hierarchical levels in each city. Dots represent the observed data; boxes indicate the standard deviation of the SES obtained from the null model. In both cities, the population variation in relation to the community variation (TIP/IC) for all traits had a lower mean and variation than expected under the null model, differently of TIC/IR and TPC/PR. This demonstrates that interspecific and regional-scale variability were greater in the urban gradient. Figure 4. Partitioning of the phenotypic variability into hierarchical levels. All phenotypic traits had little variation between ponds (< 0.001) and appear as 0% in the figure. The variability for genus, species. and region is greater for all phenotypic traits. Relative leg length had high variability at the population scale (within) along the urban gradient. Long-time urbanized Recently urbanized Habitat fragmentation Low effect High effect Changes in phenotypic variability High genetic drift Decrease in population size Low gene flow Do urban environments restrict phenotypic variability phenotypic traits related to the impact and requirement niche? At Intraspecific and interespecific scales Phenotypic adaptive changes Genetic adaptive changes to urban environments São José dos Campos, SP, Brazil Campo Grande, MS, Brazil Environmental variables at the landscape scale raster sf gtools terra tidyr 37 years 2022 1985 T statistics IP = Intra-population variability IC = Intra-community variability IC = Intra-community variability IR = Intra-regional pool variability PC = Variability in population means PR = Variability in community means Null models (local and regional pool) Hierarchical levels (population, species, community, regiona


The author acknowledges the General Director of Department of Fisheries Malaysia, Economic Planning Unit (EPU) for the funding support, the Director of the Fisheries Research Institute, Director of FRI Bintawa, Director of JPLS and Director of FRI Kampung Acheh, and to the officers and staffs of FRI for the hard work and cooperation. Acknowledgement 2020@Copyright Reserved Disediakan oleh: Jamil Musel Mohammad Hafiz Hassan Arfazieda Anuar Time of Fishing Operation The operation shall be conducted either during the day or night. The operation will be conducted 4 times in 24 hours with total magazine at least 20 units. Each operation takes about 3 hours. Bear in mind that the fishing operation is depends on the weather condition, safety in operating and landings. Fishing Depth Usually, 95-180 metres is the preferred depth for longline fishing. Fishing ground depends on the seabed condition, such as rocky, muddy, sandy and seagrass areas are desired. As a reminder, the artificial reef area shall be avoided as it is a conservation area. Shooting The shooting operation will be conducted in1 hour for 5 kilometre per operation. The fishing depth will increase with the increasing number of magazine used. Boat Speed/Direction Boat speed shall remain consistent during shooting at 1-2 knot. During hauling, the speed shall be decelerated to 1.5 knot. Type of Bait Bait can be minced or used as a whole. Bait type is usually barracuda size 20-22 cm long or squid, cut into pieces, hooked and placed in boxes. The bait- hooking process is automatically performed by the ABLL itself as the boat move forward. As the boat arrives at a desired fishing area, the flagpole will be tossed out into the sea as a starting fishing position indicator. Consequently, a sinker will be thrown out to the sea, followed by a sets of hooks until the end of the magazine, where another sinker is attached. Recording of Start Shooting Time The time when any part of the gear reaches the sea. Data Recording for Location Surveys The data and location are both recorded as below: • Record position for each station using Global Positioning System • Record position and start and stop times for shooting • Record position and start and stop times for hauling Target Species Target species are the demersal fishes from the family Carangidae, Serranidae, Lutjanidae and Nemipteridae and a small amount of pelagic fishes. The Recording of Finish Hauling Time The time when the operator hauled all part of gear on-board. Setting distance shall be calculated from position of start shooting and finish shooting compare with length of mainline deployed. Recording of Finish Shooting Time The time when the last part of the gear shot overboard. Recording of Start Hauling Time The time when operator hauled any part of gear on- board. Water inlet system will be set off and flows into the baiter to prevent line entangling. ABLL full body assemble and ready for fishing operation. Recording of Fishing Position Fishing position shall be recorded by using the GPS or an equally accurate navigation system. Position recording will be in Latitude and Longitude format. Gear malfunction Malfunctioning of fishing gear usually due to the disconnected rope or entangling of mainline under water during hauling operation. The details of entangling of longline shall be detected using floats that connected to the mainline of the longline. The position and station numbers shall be recorded. Recording of Start Fishing Position The position that any part of the gear reaches the sea. Recording of Finish Fishing Position The position that last part of the gear shot overboard. ABLL is a kind of stationary and long fishing gear, so that information of start hauling position and finish hauling position are required. Then the hooks will move one after another as the boat moves forward and the fishing operation starts. The baits will be deposited into the empty baiter together with water. Bait Indicator of Abundance Other dat


Two main experiments were conducted for this study: Hot stage microscopy −Mixture: raw material : slag = 1:1 weight ratio −Shape: small cube of 3 mm side −Heating: 10°C/min up to 1500°C −Computer treatment: measure of height of sample Heat treatment of pellets −Mixture: raw material : slag = 3:1 weight ratio −Pellets: Ø20 mm, pressed at 80 MPa −Heat treatment: 5°C/min, dwell 1h at 1400°C −Observation by SEM with EDS ESR Position 04 CHARACTERISTIC TEMPERATURE DETERMINATION OF REFRACTORY RAW MATERIALS AND SLAG MIXTURES Camille REYNAERT Early Stage Researcher mail: reynaert@agh.edu.pl Advanced THermomechanical multiscale mOdelling of Refractory Linings Supervisors & University Consortium of 15 European Partners Edyta ŚNIEŻEK Authors: Camille REYNAERT, Edyta ŚNIEŻEK, Jacek SZCZERBA Address: AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Krakow, Poland Materials: Introduction: Conclusions: Steel industry is the most important consumer of refractory bricks. Refractories are commonly used as the linings of steel equipment because they can sustain very high temperature and corrosive attacks in the working environment. However due to the harsh working condition, their life span is limited. Corrosion mainly by slag is the principal phenomenon responsible for the degradation and wear of the bricks. Acknowledgments This project has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement no.764987 The materials used are typical powders that can be found in refractories for steel making applications and slags pick up from a steel ladle. The powder is calcium zirconate with composition given in table 1. The slags are silica rich slags. They were taken at different moment of the treatment of a batch of steel in the ladle. They composition is given in table 2 and they equilibria in a phase diagram in the figure 2. Table 2: Composition of the slags obtained by XRF. Table 1: Composition of the powder obtained by XRD. Figure 2: Curves obtain by hot stage microscopy for calcium zirconate. Jacek SZCZERBA Calcium zirconate corroded by different slags exhibit different behaviors. This is particularly noticeable between the first mixture (CaZrO3 + SiS1) and the others. This is due the major difference in composition between SiS1 and the others slags (SiS2/3/4). When using phase equilibria diagram, one can notice that SiS1 is in another equilibria zone compare to SiS2/3/4 (figure 1). MgO SiO2 SiS2/3/4 SiS1 Results: Expansion Shrinkage Initial softening Flowing CaZrO3 Start End Start End + SIS1 100 1080 1080 1380 1400 1460 + SiS2 100 960 960 1410 1410 / + SiS3 100 920 1080 1400 1420 / + SiS4 100 940 940 1400 1400 / • For SiS1, the matrix melted and grains were partly dissolved as they appear rounded. • For SiS2/3/4, only part of the slag melted but grains were not attacked: almost no rounded grains. The different characteristics temperatures for each mixture are given in table 3. The observed differences can be explained by the formation of different phases in the mixture and differences in microstructure as is observed in Fig 3a and b. CaZrO3 + SiS1 CaZrO3 + SiS2 CaZrO3 + SiS3 CaZrO3 + SiS4 Calcim zirconate - CaZrO3 Zirconium oxide - ZrO2 Spinel - Mg8Al16O32 Mn3O4 Gehlenite - Ca4Al4Si2O14 Braunite - Mn56Si8O96 Grossular - Ca24Al16Si24O96 Calcim zirconate - CaZrO3 Zirconium oxide - ZrO2 Periclase - MgO β-Ca2SiO4 – Monoclinic Oldhamite - CaS Ca16Si8F8O28 Calcim zirconate - CaZrO3 Zirconium oxide - ZrO2 Periclase - MgO β-Ca2SiO4 – Monoclinic Oldhamite - CaS Ca16Si8F8O28 Calcim zirconate - CaZrO3 Zirconium oxide - ZrO2 Periclase - MgO β-Ca2SiO4 – Monoclinic Oldhamite - CaS Ca16Si8F8O28 Pyroxene ideal - MgSiO3 The phase compositions help to understand the phenomena that took places. For the first mixtures, gehlenite melts around 1250°C which explains the sudden drop in volume which in then sustain by the dissolution of zirconate grain in the slag as can be observed


Identifying new functions of the EGF/ EGFR pathway through tissue-speciﬁc recombination Silvan Spiri, Louisa Mereu, Matthias Morf, Alex Hajnal Institute of Molecular Life Sciences, University of Zurich LIN-3 EGF LET-23 EGFR AC P4.p P5.p P6.p P7.p P8.p P3.p Basement membrane 1° LIN-3 EGF LET-23 EGFR AC P4.p P5.p P6.p P7.p P8.p P3.p Basement membrane A) EGF/ LIN-3 dependent induction of the vulval precursor cells. VulE VulE VulF VulF AC VulF VulF VulE VulE C) The AC directly promotes dorsal lumen formation during vulva morphogenesis. Ventral nerve cord Basement membrane 2° 2° 1° 1° 1° 1° UNC-6 FOS-1 ? ? B) The anchor cell (AC), guided by Netrin/ UNC-6 and unkown VPC guidance cue`s, breaches the basement membrane after the VPCs divided the second time. FRT GFP let-23::gfp let-23 let-23::gfp FRT Protein kinase domain FRT GFP E) Two FRT sites, ﬂanking the sole kinase domain of egfr/ let-23, along with a GFP sequence are inserted into the endogenous egfr/ let-23 locus. Upon Flp expression the kinase domain is excised resulting in in- active receptor and loss of GFP signal. Tool to induce a temporal or spatial speciﬁc knock-out of egfr/ let-23: ! " VulE VulE VulF VulF AC VulF VulF VulE VulE utse utse utse utse utse LET-23 EGFR LIN-3 EGF uv1 cell fate uv1 utse uv1 D) EGF/ LIN-3 is expressed in the 1° vulval cell lineage after vulva induction and is necessary to specify the uterin uv1 cell fate. Introduction Model Summary VulE VulE VulF VulF VulF VulF VulE VulE AC LET-23 EGFR LIN-3 EGF EGFR is expressed in the AC from the onset of vulval mor- phogenesis, securing dorsal lumen formation by stabilizing cytoskeleton components during lumen opening 1) EGFR is expressed in the AC during vulval morphogenesis 2) AC mispositioned in AC EGFR-KO animals during L4.1-2 3) F-actin in AC is disorganized during L4.2-3 in AC EGFR-KOs 4) In a sensitized background BM breaching during morpho- genesis is aﬀected in AC EGFR-KO animals 5) Narrow dorsal lumen upon AC EGFR-KO during L4.4 6) KO of lin-3 in the VPCs or let-23 in the AC leads to egg laying defective adults let-23 egf X egfrX 3) KO of egfr/ let-23 in the AC leads to more variability in AC alignment during L4.1 and L4.2 4) KO of egfr/ let-23 in the AC leads to disorganized F-actin network in L4.2 6) KO of egfr/ let-23 in the AC leads to a decreased dorsal lumen diameter during L4.4 7) KO of lin-3 in the VPCs or let-23 in the AC leads to egg-laying defectie animals Ventral nerve cord Basement membrane 2° 2° 1° 1° 1° 1° UNC-6 FOS-1 ? ? Vulval development and morphogenesis: let-23::gfp Ac::Flp; Nomarski Ctrl; Nomarski let-23::gfp Anchor cell Alignment L4.1 ns p=0.0086 p=0.0005 0 1µm 2µm 3µm 4µm Ctrl Ac::Flp Ctrl Ac::Flp KO Anchor cell Alignment L4.2 p=0.0024 ns p=0.0120 Ctrl Ac::Flp Ctrl Ac::Flp KO 0 1µm 2µm 3µm 4µm LET-23::GFP signal is lost upon AC speciﬁc KO of egfr/ let-23 resulting in a more variable position of the AC during L4.1 and L4.2: (A) schematic of AC speciﬁc KO of egfr/ let-23. (B) Example images of AC speciﬁc KO animals during L4.1 on the left and control animals on the right. Upper row Nomarski images, bottom row LET-23::GFP. Scale bar represents 5 µm, arrowhead indicates the AC. (C) Quantiﬁaction of AC alignment relative to the vulval midline during L4.1 and L4.2. A) B) C) Ac::Flp; Nomarski Ctrl; Nomarski AJM-1::GFP AJM-1::GFP AC speciﬁc KO of egfr/ let-23 leads to a smaller dorsal lumen diameter during L4.4: (A) Example images of AC speciﬁc KO animals during L4.4 on the left and control animals on the right. Upper row Nomarski images, bottom row AJM-1::GFP to mark the apical junctions. (B) the dorsal lumen diameter was determed by measuring the inner diameter of the vulF toroid using the vulF/ vulE apical junctions. A) B) L4.4 Dorsal lumen diameter p=0.0002 Ctrl n=22 Ac::Flp n=22 9µm 8µm 7µm 6µm 5µm 4µm 3µm 2µm 1µm 5) KO of egfr/ let-23 in the AC leads to decreased base- ment membrane breaching during morphogenesis in a sensitized background A) B) egfr/ let-23 KO increases failure


X-chromosome Inactivation In humans one of the two X chromosomes in females is inactivated, but some genes on the inactive X escape inactivation (Carrel and Willard, 2005; Figure 4). If X chromosome inactivation (XCI) evolves in response to loss of gene content on the Y chromosome, then XCI status should be a strong predictor of the status of Y-linked homologs. Comparing Classes For each gene data was collected on several possible predictive features including expression intensity and expression breadth across 79 human tissues (Su et al., 2004), GO functional categories, association with human disease in Online Mendelian Inheritance of Man, and selective constraint at nucleotide sites. For each feature, each class of genes was compared using a permutation test with 1000 iterations to detect differences between the classes. There were no significant differences between any classes with respect to function, expression or selective constraint. Learning from genetic fossils on the Y chromosome Acknowledgments Many thanks to the Mohnkern Scholarship and the Miller Institute for Basic Research in Science for supporting this research. M.A. Wilson Sayres and K.D. Makova Sex Chromosome Evolution Mammalian X and Y evolved from a pair of homologous autosomes (Fig. 1). In the absence of X- Y recombination, caused by a series of inversions on the Y chromosome, the Y lost much of its gene content. Are some types of genes more likely to be lost? Is it possible to predict the fate of Y-linked genes? Figure 2. Existence of a gene in homologous regions across species was used as evidence for the presence of that gene on the ancestral X. Figure 1. Some strata (labeled with numbers) are shared between mammals while some are species-specific. This means that Y-evolution is unique to each species and highlights the need for a general understanding of how Y chromosomes evolve. Figure 3. Proportion of X-linked genes with functional (black), pseudogenized (grey), or lost (white) Y homologs along chrX in 5Mb windows. Table 1. Results of LDA. Predicting Y retention and loss Using a linear discriminant analysis (LDA) with XCI status as the sole predictor, the model successfully predicts whether and X-linked gene has a functional Y homolog or has lost its Y homolog, with intermediate success predicting whether the Y is a pseudogene (Figure 5 and Table 1). Figure 5. Significant differences in X-inactivation status between classes of X-linked genes. Conclusions • Current function, expression and selective constraint of X-linked genes were not significantly different between any of the classes. It may be that the signals have diminished over time. • X-chromosome inactivation results from gene loss on the Y. • XCI status can correctly predict the status of Y-linked homologs References Carrel and Willard (2005) Nature, 434(7031): 400-4. Lahn and Page (1999) Science, 286(5441): 964-7. Su et al. (2004) PNAS, 101(10): 6062-7. Classifying X-linked genes Genes present on the ancestral X chromosome (Figure 2) were categorized into three classes (Figure 3). We identified hundreds of previously undescribed Y-linked pseudogene sequences using lastZ. Figure 4. X-inactivation in females mirrors gene loss on the Y chromosome in males. X-linked gene class Correct LDA Classification Functional Y homolog 85% Pseudogenized Y homolog 56% Lost Y homolog 92%


KIT – The Research University in the Helmholtz Association Expected angular distribution. D. Hinz, poster #352 scintillation photons detected electron transmitted electron scintillator magnetic field lines cell size ca. 150 μm x 150 μm x 300 μm Design of a Scintillating Active Transverse Energy Filter (scint-aTEF) for Background Suppression at the KATRIN Experiment Nathanael Gutknecht for the KATRIN Collaboration Institute of Experimental Particle Physics (ETP) We acknowledge the support of Helmholtz Association (HGF), Ministry for Education and Research BMBF, the doctoral school KSETA at KIT, Helmholtz Initiative and Networking Fund, Max Planck Research Group, and DFG in Germany; Ministry of Education, Youth and Sport in the Czech Republic; INFN in Italy; the National Science, Research and Innovation Fund via the Program Management Unit for Human Resources & Institutional Development, Research and Innovation in Thailand; and the DOE Office of Science, Nuclear Physics in the United States. This project has received funding from the ERC under the European Union Horizon 2020 research and innovation programme. We thank the computing cluster support at the Institute for Astroparticle Physics at KIT, Max Planck Computing and Data Facility (MPCDF), and the National Energy Research Scientific Computing Center (NERSC) at LBNL. calibration remove tritium e– from T2 decay filter electrons by their energy count events KATRIN Collaboration, adapted The Karlsruhe Tritium Neutrino Experiment (KATRIN) Measurement principle for  Measure kinetic energy of e–  Look for distortion of energy spectrum near endpoint caused by neutrino mass  Goal: < 0.3 eV / c² (90 % C.L.) Concept of the scint-aTEF Simulations for Optimal Geometry Angular detection efficiency  Purely based on Geometry ROC curve for bkg-suppression  assuming 30 % Rydberg fraction Expected improvement on  KNM2-like scenario  assumes scint-aTEF from day zero Conclusion  Valid concept in case of large angular separation between β- and background e– (Rydberg electrons)  good light yield of scintillator is essential  Implementation of scint-aTEF in KATRIN unlikely due to long development time Development of the scint-aTEF 3d printed plastic scintillator  2 photon polymerization at μm scale at KIT-APH Readout: SPAD arrays  “IDP4” from ZITI (Heidelberg) for single e– event detection J. Lauer P. Kiefer 3d printed microstructure, non scintillating. From https://doi.org/10.5445/IR/1000167180 Single electron event (top left), captured with a SPAD array and a commercial scintillator. Discriminate keV- electrons by their cyclotron motion  β-electrons: large angles (isotropic)  background: small angles